LOCUS       DACDTO010000001       101634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDTO010000001 DACDTO010000000
VERSION     DACDTO010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 101634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.001"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(12..1535)
                     /locus_tag="IWK70_000001"
     CDS             complement(12..1535)
                     /locus_tag="IWK70_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9612584.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="IWK70_000002"
     CDS             1787..2968
                     /locus_tag="IWK70_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9612585.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IWK70_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="IWK70_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9612586.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="IWK70_000004"
     CDS             complement(5053..7764)
                     /locus_tag="IWK70_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9612587.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="IWK70_000005"
     CDS             complement(7774..8475)
                     /locus_tag="IWK70_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9612588.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="IWK70_000006"
     CDS             8661..9260
                     /locus_tag="IWK70_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9612589.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="IWK70_000007"
     CDS             9341..10354
                     /locus_tag="IWK70_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9612590.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="IWK70_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="IWK70_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9612591.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="IWK70_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="IWK70_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9612592.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="IWK70_000010"
     CDS             11802..13175
                     /locus_tag="IWK70_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9612593.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="IWK70_000011"
     CDS             13532..15676
                     /locus_tag="IWK70_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9612594.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="IWK70_000012"
     CDS             complement(15728..16768)
                     /locus_tag="IWK70_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9612595.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="IWK70_000013"
     CDS             complement(17045..18847)
                     /locus_tag="IWK70_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9612596.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="IWK70_000014"
     CDS             complement(18861..19613)
                     /locus_tag="IWK70_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9612597.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="IWK70_000015"
     CDS             complement(19622..19984)
                     /locus_tag="IWK70_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9612598.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="IWK70_000016"
     CDS             complement(20160..21032)
                     /locus_tag="IWK70_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9612599.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="IWK70_000017"
     CDS             complement(21058..21570)
                     /locus_tag="IWK70_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9612600.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22538)
                     /locus_tag="IWK70_000018"
     CDS             complement(21708..22538)
                     /locus_tag="IWK70_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9612601.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            complement(22554..23162)
                     /locus_tag="IWK70_000019"
     CDS             complement(22554..23162)
                     /locus_tag="IWK70_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9612602.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="IWK70_000020"
     CDS             complement(23155..23472)
                     /locus_tag="IWK70_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9612603.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="IWK70_000021"
     CDS             complement(23628..24356)
                     /locus_tag="IWK70_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9612604.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="IWK70_000022"
     CDS             24515..25396
                     /locus_tag="IWK70_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9612605.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="IWK70_000023"
     CDS             complement(25633..26676)
                     /locus_tag="IWK70_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9612606.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="IWK70_000024"
     CDS             complement(26679..27347)
                     /locus_tag="IWK70_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9612607.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="IWK70_000025"
     CDS             27489..29825
                     /locus_tag="IWK70_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9612608.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="IWK70_000026"
     CDS             complement(29940..30251)
                     /locus_tag="IWK70_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612609.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="IWK70_000027"
     CDS             30432..31907
                     /locus_tag="IWK70_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9612610.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="IWK70_000028"
     CDS             31954..32538
                     /locus_tag="IWK70_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9612611.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="IWK70_000029"
     CDS             32540..32959
                     /locus_tag="IWK70_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9612612.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="IWK70_000030"
     CDS             complement(33240..33524)
                     /locus_tag="IWK70_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9612613.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="IWK70_000031"
     CDS             complement(33876..34586)
                     /locus_tag="IWK70_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9612614.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="IWK70_000032"
     CDS             complement(34583..36055)
                     /locus_tag="IWK70_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9612615.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="IWK70_000033"
     CDS             36193..36666
                     /locus_tag="IWK70_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9612616.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="IWK70_000034"
     CDS             complement(36819..37370)
                     /locus_tag="IWK70_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9612617.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="IWK70_000035"
     CDS             37475..38005
                     /locus_tag="IWK70_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9612618.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="IWK70_000036"
     CDS             complement(38488..38931)
                     /locus_tag="IWK70_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9612619.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="IWK70_000037"
     CDS             complement(38947..40107)
                     /locus_tag="IWK70_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9612620.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="IWK70_000038"
     CDS             40614..42314
                     /locus_tag="IWK70_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612621.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="IWK70_000039"
     CDS             complement(42416..43249)
                     /locus_tag="IWK70_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9612622.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="IWK70_000040"
     CDS             complement(43553..45718)
                     /locus_tag="IWK70_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9612623.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="IWK70_000041"
     CDS             45978..48272
                     /locus_tag="IWK70_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9612624.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="IWK70_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="IWK70_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9612625.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="IWK70_000043"
     CDS             48923..49930
                     /locus_tag="IWK70_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9612626.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="IWK70_000044"
     CDS             49952..50971
                     /locus_tag="IWK70_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9612627.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IWK70_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="IWK70_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9612628.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="IWK70_000046"
     CDS             complement(52729..54150)
                     /locus_tag="IWK70_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9612629.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IWK70_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="IWK70_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9612630.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="IWK70_000048"
     CDS             complement(56561..57817)
                     /locus_tag="IWK70_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9612631.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            58397..59698
                     /locus_tag="IWK70_000049"
     CDS             58397..59698
                     /locus_tag="IWK70_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9612632.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="IWK70_000050"
     CDS             complement(59767..60366)
                     /locus_tag="IWK70_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9612633.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="IWK70_000051"
     CDS             60620..61501
                     /locus_tag="IWK70_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9612634.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="IWK70_000052"
     CDS             61514..63124
                     /locus_tag="IWK70_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9612635.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="IWK70_000053"
     CDS             complement(63201..63860)
                     /locus_tag="IWK70_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9612636.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="IWK70_000054"
     CDS             complement(64143..64808)
                     /locus_tag="IWK70_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612637.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IWK70_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="IWK70_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9612638.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IWK70_000056"
     CDS             complement(65588..66571)
                     /gene="dhaK"
                     /locus_tag="IWK70_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9612639.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IWK70_000057"
     CDS             complement(66573..66968)
                     /gene="dhaM"
                     /locus_tag="IWK70_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9612640.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="IWK70_000058"
     CDS             67122..67442
                     /locus_tag="IWK70_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9612641.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="IWK70_000059"
     CDS             complement(67554..68423)
                     /locus_tag="IWK70_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9612642.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69793)
                     /locus_tag="IWK70_000060"
     CDS             complement(68519..69793)
                     /locus_tag="IWK70_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9612643.1"
                     /translation="MLRYLFVYYVFFLVSYPILGGFVWFIGVWCYVFLYKHKQKEWVD
                     VPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGSTDQTPEILARL
                     MKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDALIRYVNYFIRP
                     GARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLYAYSGANTMYR
                     KEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTLKMLYRQRKRW
                     AKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVLFAGLIGHYVY
                     QGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFAPLYMLLFWIV
                     NAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(69819..70328)
                     /locus_tag="IWK70_000061"
     CDS             complement(69819..70328)
                     /locus_tag="IWK70_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612644.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="IWK70_000062"
     CDS             complement(70503..72638)
                     /locus_tag="IWK70_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9612645.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="IWK70_000063"
     CDS             complement(72721..74376)
                     /locus_tag="IWK70_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9612646.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="IWK70_000064"
     CDS             complement(74378..74692)
                     /locus_tag="IWK70_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9612647.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="IWK70_000065"
     CDS             74862..75122
                     /locus_tag="IWK70_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9612648.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="IWK70_000066"
     CDS             75136..75450
                     /locus_tag="IWK70_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9612649.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="IWK70_000067"
     CDS             complement(76180..76734)
                     /locus_tag="IWK70_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9612650.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="IWK70_000068"
     CDS             complement(76860..78788)
                     /locus_tag="IWK70_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9612651.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="IWK70_000069"
     CDS             complement(79094..79810)
                     /locus_tag="IWK70_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9612652.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="IWK70_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="IWK70_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9612653.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="IWK70_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="IWK70_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9612654.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="IWK70_000072"
     CDS             complement(83280..84740)
                     /locus_tag="IWK70_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9612655.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="IWK70_000073"
     CDS             complement(84730..86025)
                     /locus_tag="IWK70_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9612656.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="IWK70_000074"
     CDS             complement(86357..87034)
                     /locus_tag="IWK70_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9612657.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="IWK70_000075"
     CDS             complement(87064..88155)
                     /locus_tag="IWK70_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9612658.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="IWK70_000076"
     CDS             complement(88256..88690)
                     /locus_tag="IWK70_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9612659.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="IWK70_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="IWK70_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9612660.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="IWK70_000078"
     CDS             complement(90728..92623)
                     /locus_tag="IWK70_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9612661.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="IWK70_000079"
     CDS             complement(92681..94456)
                     /locus_tag="IWK70_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9612662.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="IWK70_000080"
     CDS             complement(94506..96002)
                     /locus_tag="IWK70_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9612663.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="IWK70_000081"
     CDS             complement(96196..97818)
                     /locus_tag="IWK70_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9612664.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="IWK70_000082"
     CDS             complement(98267..99580)
                     /locus_tag="IWK70_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9612665.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="IWK70_000083"
     CDS             complement(99825..100049)
                     /locus_tag="IWK70_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612666.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100437)
                     /locus_tag="IWK70_000084"
     CDS             complement(100069..100437)
                     /locus_tag="IWK70_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9612667.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="IWK70_000085"
     CDS             complement(100635..101345)
                     /locus_tag="IWK70_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612668.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..>101634)
                     /locus_tag="IWK70_000086"
     CDS             complement(101363..>101634)
                     /locus_tag="IWK70_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9612669.1"
                     /translation="EEMADHFLEIERIYVRKKFKHQGIGTFLLDTAIEKAKEKQKTAI
                     WLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLFIKQLF"
BASE COUNT        29836 a        21151 c        17889 g        32758 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggac ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcag
//
LOCUS       DACDTO010000002        86690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDTO010000002 DACDTO010000000
VERSION     DACDTO010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.002"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..249)
                     /locus_tag="IWK70_000087"
     CDS             complement(<1..249)
                     /locus_tag="IWK70_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9612670.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFII"
     gene            432..1556
                     /locus_tag="IWK70_000088"
     CDS             432..1556
                     /locus_tag="IWK70_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9612671.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            1783..2700
                     /locus_tag="IWK70_000089"
     CDS             1783..2700
                     /locus_tag="IWK70_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9612672.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            2693..3526
                     /locus_tag="IWK70_000090"
     CDS             2693..3526
                     /locus_tag="IWK70_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9612673.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            3609..4727
                     /locus_tag="IWK70_000091"
     CDS             3609..4727
                     /locus_tag="IWK70_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9612674.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            4721..6619
                     /locus_tag="IWK70_000092"
     CDS             4721..6619
                     /locus_tag="IWK70_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9612675.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            6613..7836
                     /locus_tag="IWK70_000093"
     CDS             6613..7836
                     /locus_tag="IWK70_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9612676.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(7922..9856)
                     /locus_tag="IWK70_000094"
     CDS             complement(7922..9856)
                     /locus_tag="IWK70_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9612677.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            10082..10738
                     /locus_tag="IWK70_000095"
     CDS             10082..10738
                     /locus_tag="IWK70_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9612678.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(10812..11165)
                     /locus_tag="IWK70_000096"
     CDS             complement(10812..11165)
                     /locus_tag="IWK70_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9612679.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            11357..12286
                     /locus_tag="IWK70_000097"
     CDS             11357..12286
                     /locus_tag="IWK70_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9612680.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            12297..13133
                     /locus_tag="IWK70_000098"
     CDS             12297..13133
                     /locus_tag="IWK70_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9612681.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            13381..14157
                     /locus_tag="IWK70_000099"
     CDS             13381..14157
                     /locus_tag="IWK70_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9612682.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            14333..15076
                     /locus_tag="IWK70_000100"
     CDS             14333..15076
                     /locus_tag="IWK70_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9612683.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            15069..16649
                     /locus_tag="IWK70_000101"
     CDS             15069..16649
                     /locus_tag="IWK70_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9612684.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(16900..17379)
                     /locus_tag="IWK70_000102"
     CDS             complement(16900..17379)
                     /locus_tag="IWK70_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9612685.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(17395..18147)
                     /locus_tag="IWK70_000103"
     CDS             complement(17395..18147)
                     /locus_tag="IWK70_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9612686.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            18264..18334
                     /locus_tag="IWK70_000104"
     tRNA            18264..18334
                     /locus_tag="IWK70_000104"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18296..18298,aa:Gly,seq:ccc)
     gene            18695..19420
                     /locus_tag="IWK70_000105"
     CDS             18695..19420
                     /locus_tag="IWK70_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9612687.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            19675..19941
                     /locus_tag="IWK70_000106"
     CDS             19675..19941
                     /locus_tag="IWK70_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9612688.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            19938..20369
                     /gene="ruvX"
                     /locus_tag="IWK70_000107"
     CDS             19938..20369
                     /gene="ruvX"
                     /locus_tag="IWK70_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9612689.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            20394..20699
                     /locus_tag="IWK70_000108"
     CDS             20394..20699
                     /locus_tag="IWK70_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9612690.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(20780..21925)
                     /locus_tag="IWK70_000109"
     CDS             complement(20780..21925)
                     /locus_tag="IWK70_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9612691.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21986..22633)
                     /locus_tag="IWK70_000110"
     CDS             complement(21986..22633)
                     /locus_tag="IWK70_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9612692.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            22827..23120
                     /locus_tag="IWK70_000111"
     CDS             22827..23120
                     /locus_tag="IWK70_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612693.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            23164..23475
                     /locus_tag="IWK70_000112"
     CDS             23164..23475
                     /locus_tag="IWK70_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612694.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            23472..24096
                     /locus_tag="IWK70_000113"
                     /pseudo
     CDS             23472..24096
                     /locus_tag="IWK70_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            24396..24758
                     /locus_tag="IWK70_000114"
     CDS             24396..24758
                     /locus_tag="IWK70_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612695.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            24795..25643
                     /locus_tag="IWK70_000115"
     CDS             24795..25643
                     /locus_tag="IWK70_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9612696.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            25633..27108
                     /locus_tag="IWK70_000116"
     CDS             25633..27108
                     /locus_tag="IWK70_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9612697.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            27374..27781
                     /locus_tag="IWK70_000117"
     CDS             27374..27781
                     /locus_tag="IWK70_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9612698.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            27784..27999
                     /locus_tag="IWK70_000118"
     CDS             27784..27999
                     /locus_tag="IWK70_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612699.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            28003..28383
                     /locus_tag="IWK70_000119"
     CDS             28003..28383
                     /locus_tag="IWK70_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9612700.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            28517..28672
                     /locus_tag="IWK70_000120"
     CDS             28517..28672
                     /locus_tag="IWK70_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9612701.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            28741..29214
                     /locus_tag="IWK70_000121"
     CDS             28741..29214
                     /locus_tag="IWK70_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9612702.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            29211..30905
                     /locus_tag="IWK70_000122"
     CDS             29211..30905
                     /locus_tag="IWK70_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9612703.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            30871..31056
                     /locus_tag="IWK70_000123"
     CDS             30871..31056
                     /locus_tag="IWK70_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612704.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            31060..32235
                     /locus_tag="IWK70_000124"
     CDS             31060..32235
                     /locus_tag="IWK70_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9612705.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            32228..33751
                     /locus_tag="IWK70_000125"
     CDS             32228..33751
                     /locus_tag="IWK70_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9612706.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            33807..34091
                     /locus_tag="IWK70_000126"
     CDS             33807..34091
                     /locus_tag="IWK70_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9612707.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            34078..34413
                     /locus_tag="IWK70_000127"
     CDS             34078..34413
                     /locus_tag="IWK70_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9612708.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            34708..35334
                     /locus_tag="IWK70_000128"
     CDS             34708..35334
                     /locus_tag="IWK70_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612709.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            36076..36913
                     /locus_tag="IWK70_000129"
                     /pseudo
     CDS             36076..36913
                     /locus_tag="IWK70_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            36978..37412
                     /locus_tag="IWK70_000130"
     CDS             36978..37412
                     /locus_tag="IWK70_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9612710.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(37480..38802)
                     /locus_tag="IWK70_000131"
     CDS             complement(37480..38802)
                     /locus_tag="IWK70_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9612711.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(38956..40596)
                     /locus_tag="IWK70_000132"
     CDS             complement(38956..40596)
                     /locus_tag="IWK70_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9612712.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            40926..41396
                     /locus_tag="IWK70_000133"
     CDS             40926..41396
                     /locus_tag="IWK70_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9612713.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            42025..43128
                     /locus_tag="IWK70_000134"
     CDS             42025..43128
                     /locus_tag="IWK70_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9612714.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            43412..44092
                     /locus_tag="IWK70_000135"
     CDS             43412..44092
                     /locus_tag="IWK70_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9612715.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            44182..45216
                     /locus_tag="IWK70_000136"
     CDS             44182..45216
                     /locus_tag="IWK70_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9612716.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            45230..45565
                     /locus_tag="IWK70_000137"
     CDS             45230..45565
                     /locus_tag="IWK70_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612717.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            45549..48029
                     /locus_tag="IWK70_000138"
     CDS             45549..48029
                     /locus_tag="IWK70_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612718.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            48085..49644
                     /locus_tag="IWK70_000139"
     CDS             48085..49644
                     /locus_tag="IWK70_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9612719.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            49641..51005
                     /locus_tag="IWK70_000140"
     CDS             49641..51005
                     /locus_tag="IWK70_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9612720.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            51176..52246
                     /locus_tag="IWK70_000141"
     CDS             51176..52246
                     /locus_tag="IWK70_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9612721.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            52261..52929
                     /locus_tag="IWK70_000142"
     CDS             52261..52929
                     /locus_tag="IWK70_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9612722.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            52949..53527
                     /locus_tag="IWK70_000143"
     CDS             52949..53527
                     /locus_tag="IWK70_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9612723.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            53930..54841
                     /locus_tag="IWK70_000144"
     CDS             53930..54841
                     /locus_tag="IWK70_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9612724.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(55097..56014)
                     /locus_tag="IWK70_000145"
     CDS             complement(55097..56014)
                     /locus_tag="IWK70_000145"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9612725.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            56216..56653
                     /gene="zapA"
                     /locus_tag="IWK70_000146"
     CDS             56216..56653
                     /gene="zapA"
                     /locus_tag="IWK70_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9612726.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            56711..57258
                     /locus_tag="IWK70_000147"
                     /pseudo
     CDS             56711..57258
                     /locus_tag="IWK70_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            57373..59733
                     /locus_tag="IWK70_000148"
     CDS             57373..59733
                     /locus_tag="IWK70_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9612727.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            59820..60134
                     /gene="trxA"
                     /locus_tag="IWK70_000149"
     CDS             59820..60134
                     /gene="trxA"
                     /locus_tag="IWK70_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9612728.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            60316..62094
                     /gene="uvrC"
                     /locus_tag="IWK70_000150"
     CDS             60316..62094
                     /gene="uvrC"
                     /locus_tag="IWK70_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9612729.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            62239..63138
                     /locus_tag="IWK70_000151"
     CDS             62239..63138
                     /locus_tag="IWK70_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9612730.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            63131..64393
                     /locus_tag="IWK70_000152"
     CDS             63131..64393
                     /locus_tag="IWK70_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9612731.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            64556..66454
                     /locus_tag="IWK70_000153"
     CDS             64556..66454
                     /locus_tag="IWK70_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9612732.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(66753..67472)
                     /locus_tag="IWK70_000154"
     CDS             complement(66753..67472)
                     /locus_tag="IWK70_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9612733.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            67809..68141
                     /locus_tag="IWK70_000155"
     CDS             67809..68141
                     /locus_tag="IWK70_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612734.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            68128..70131
                     /locus_tag="IWK70_000156"
     CDS             68128..70131
                     /locus_tag="IWK70_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9612735.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            70150..70620
                     /locus_tag="IWK70_000157"
     CDS             70150..70620
                     /locus_tag="IWK70_000157"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9612736.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            70641..71225
                     /locus_tag="IWK70_000158"
     CDS             70641..71225
                     /locus_tag="IWK70_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9612737.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            71240..72226
                     /locus_tag="IWK70_000159"
     CDS             71240..72226
                     /locus_tag="IWK70_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9612738.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            72216..72527
                     /locus_tag="IWK70_000160"
     CDS             72216..72527
                     /locus_tag="IWK70_000160"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9612739.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            72549..74330
                     /locus_tag="IWK70_000161"
     CDS             72549..74330
                     /locus_tag="IWK70_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9612740.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            74323..75699
                     /locus_tag="IWK70_000162"
     CDS             74323..75699
                     /locus_tag="IWK70_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9612741.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            75706..76341
                     /locus_tag="IWK70_000163"
     CDS             75706..76341
                     /locus_tag="IWK70_000163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9612742.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            76371..77726
                     /locus_tag="IWK70_000164"
     CDS             76371..77726
                     /locus_tag="IWK70_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9612743.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(77918..79312)
                     /locus_tag="IWK70_000165"
     CDS             complement(77918..79312)
                     /locus_tag="IWK70_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9612744.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            79404..80375
                     /locus_tag="IWK70_000166"
     CDS             79404..80375
                     /locus_tag="IWK70_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9612745.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            80372..81154
                     /locus_tag="IWK70_000167"
     CDS             80372..81154
                     /locus_tag="IWK70_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9612746.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(81323..81694)
                     /locus_tag="IWK70_000168"
     CDS             complement(81323..81694)
                     /locus_tag="IWK70_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9612747.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            81828..82271
                     /locus_tag="IWK70_000169"
     CDS             81828..82271
                     /locus_tag="IWK70_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9612748.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(82314..83123)
                     /gene="yidA"
                     /locus_tag="IWK70_000170"
     CDS             complement(82314..83123)
                     /gene="yidA"
                     /locus_tag="IWK70_000170"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9612749.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(83142..84509)
                     /locus_tag="IWK70_000171"
     CDS             complement(83142..84509)
                     /locus_tag="IWK70_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9612750.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(84493..85323)
                     /locus_tag="IWK70_000172"
     CDS             complement(84493..85323)
                     /locus_tag="IWK70_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9612751.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            85486..85923
                     /locus_tag="IWK70_000173"
     CDS             85486..85923
                     /locus_tag="IWK70_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9612752.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            85999..86619
                     /locus_tag="IWK70_000174"
     CDS             85999..86619
                     /locus_tag="IWK70_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9612753.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT        28596 a        14641 c        17956 g        25497 t
ORIGIN      
        1 aatgataaaa gtttgtgttt ccttttgagt agaaaagatt tggacttctt gctgtttatc
       61 aatgaataaa agggaaggat tatctagttc tatagattta tgtgcaaatt cagcatataa
      121 attagcgctt tcatttatta gtagtcgaaa atagggagca gcagataaat gaagatgggt
      181 atctatggaa taggttagtg tttttttgac aagattggag gattggttgt ttgttaacaa
      241 aagaaccact ttcctttctc tttttatata aaaagctttc tatttataat taatgactat
      301 tataacaaaa atgaaaagcc aaaacgacaa gtaaaatatt tttttatata aatatttgtg
      361 ttactaattc cgttaatata cataagtgaa agcgttttat ataagaactt gaaaagaaag
      421 aagggataga catgaaaaaa agatacaaat tggtgatagc tttgctaaac attaccctat
      481 tattcagctt aagtgcatgt ggaaaaacta acgaagaagt aacagcaaac atcgcagatc
      541 caatgataaa aaaagccact acattagaag tatatgtcgc aaacggcaaa ctgacaaact
      601 atgttgaaaa tgctgcaaaa atctataacc gcaaaactgg agcaaatatt gacttaaaaa
      661 ttgtgaatgt agcttcggga acagcaacag tacaaatgat caccccaaaa ttggtaacac
      721 acgaagatat gccggatatt atctccatgg cagacacttc agcagcaggt gtgctggaaa
      781 aatttcaaga tgctttttat agtgctgatg aatatggatt ttatgacaaa tatggcgatc
      841 agttttatga tcaaaaattg aatattttgc gtcaacaaac gagtaatgaa acgattattc
      901 cttgggctag tgattttacc ccagcacttt gttattatca accaaaaaaa tttgctgaaa
      961 taggtgtaga ttttgaagaa atcagttctt gggaagaata tatccaggtg gcaaaagcat
     1021 taaaagaaaa aactggtagt tttggaattg ccttgccaga atctggggac caagaattgt
     1081 ttatcaacat gctagcccag cagaatcaat ctttgcttga tgaagaagga aatatccgtc
     1141 tgaatactaa agaagcgaaa catgctgcaa agttaatcaa agaaatgatc gactcagata
     1201 ttgtacattt ctacggtgca caagatgctg agaaagcatt tcaagaatct gcaatgtttg
     1261 ttgctggcgg ctggtatgct acgaatatgt ctttgaattt tcctgatgca tctgacaaat
     1321 ggcgcatggc tccactgatt cctttttcaa aagaaaatcc aggaaaaggt gcagtatcag
     1381 gaggatcaag tttttatgtc cctaaagatg ccaaaaatcc acaagtggca caacaatttc
     1441 ttacatttat gctgacagat gaagaatgct tagccaacgc actggagctg ggcgtggcta
     1501 catctaactc attggcctat caaacagaag cagctgagaa aaagtttgac tactaaggag
     1561 gacaaaaata ctatgaacta ttgaataaaa tcaatgaaaa tacatcaaat actgttttac
     1621 cagccactta ttcagatgct acgtcttatt tgactaccgc tgcatataac tattggcaaa
     1681 caggtgattt tgaaaattca tatctaaaag aagcagaaaa ttttgctcat aagtacggcg
     1741 taaaattaac tggtgattaa aaaatattag gaggacttaa cgatgatgca atcttcgtta
     1801 ccaagcaaga caataaagaa cagcaagaaa aaagcaacag atacgtctca taaaaaaagt
     1861 gcttatggat tggtcacacc ttttgttatt ttctttattg tgacgatttt gattcccatc
     1921 attatctcgt ttgcttttag ttttatgaaa attggctact catttgaatt tgttggattt
     1981 gacaattatc tagctatctt ccgagatcca ttgtttatta aatcttacac gaatgttttg
     2041 attatcatgg caggatcaat tccaataacg atgctattag ctatttactt tgctgtttta
     2101 ttgaattcag cacagttaaa aggaaaagga ttttttagaa ctgtctatta tattccaacc
     2161 attacatcaa cggtggctgt agcttccgta ttcatgactt ttttcaatcc gactgggtta
     2221 ttcaatggat ttttgaatgc gctaggaatt aacggtgtac agtggttaac aaatcctttt
     2281 tggatacgaa tatcaatgat catcacgatg atttggctta gtgtaggata taacactatt
     2341 ttatttttag ctggtcttca agggatttct aaagagatat acgaaagtgc tgaaatagat
     2401 ggagcatcga aatttcggca attcttttta ttaacgatcc ccatgctaaa gccaatcatt
     2461 ttaatggcca cagttttagc cactatcaat ggcttgggta atttcaatat cccaaatata
     2521 ttctttggga cttccaatgg accagaaaat tcagctttgg tcgtaggagt caatttatat
     2581 aaaacaagtt ttgaaatggt tgattttgga aaagcctctg ctattgcctg gacgatggtg
     2641 tttgtatctg ttatattatc ggctattcaa tttaaattcg gaggtgataa aaatgactaa
     2701 acaaaagata caaagcagaa tttttacgta cgtcattatt gtgtttggtg ccttaatatt
     2761 tttgatgcca tatttatata tgattgtagc ttcaacgcaa agtaatcaag aaattttaag
     2821 tggcggaatc aatttcaagt ttggggatat gctattaaaa aactggcaag ccatcatggc
     2881 gagatataat tatcccttga ttatttggaa ttcgattgtt attgctgtaa tcagtacagt
     2941 tttagctaca gccagttcta caatggcggg ttatgcatta gcaaagtatc gttttatggg
     3001 gaataaactg ttgttcaata cagttatgct atctaggatg gtgccttttt ttaccacatt
     3061 gattccttta ttttacatta tgagtcgttt aggattatcg aatacttatg caggtgtgat
     3121 catcccttcg attgccagta caacttcagt gtttatgatg cgacaatata gcagtcaatt
     3181 tcctacagca ctgatggagg ctgccagaat cgatggtgcc aatgagtggg tgatttttac
     3241 gaaaatcgct gtaccgattt tatcacctac gattattact agtggtttac ttatctttat
     3301 gggaagttgg aatcaatatc tatttccatt agtcatgctt tcagataaag ataaatatac
     3361 ggttccctta attatcaaaa atatgacggt ttctagtaca ggtgagccaa ttaattatgg
     3421 cgcaattatg ctaattttag caacttcagt gatcccaatg gtcttgattt atgtgtgggc
     3481 gcagtctaaa tttaaggaga atgacattgg agctgcaaat aagtaaaaaa aataacgaat
     3541 gttaaccatc tagttcgttc taggagcaac tccacattca taaaaattaa aagaatggcg
     3601 ggatatccat gaatgaacag acgaaggaat ggtcaagaaa aattttgaat gtagcttctg
     3661 aaaagataga tgcggaatta gacagaatgg ggaataaaat tccatatatt tcttacaacg
     3721 gaaaatatac agacgtaata aaggacagag gaatcgactg gtggaccaat ggtttttgga
     3781 gtggtatttt atggcagctc tttcacaaga caaaacgaga aaagttcaaa gaggcagcaa
     3841 tacaacaaga acaattatta gaatctgccc tgtaccattt ttcagacgtt catcatgatg
     3901 taggtttttt attcttacca tcggctattg cacattatca acagtctcaa aacaaacata
     3961 gtcgacaact agggttacat gcagctacgg tacttgctgg acgctataat atttcaggga
     4021 actatttagt agcatggaat gaccataaac ctggttggat gattattgat agtatgatga
     4081 atttaccatt actgtattgg gcttcgaggg aaactggtga tccgaggttc tctaaaattg
     4141 ctcggcagca tgcagataca acagctaagt atctagtgag gaaagatgga tcaaccgggc
     4201 atatcgcaga atttgatgca gaatcaggga aatatattcg gttacttggc gggcaaggat
     4261 acaatgagaa ttcttgttgg agcagaggaa atgcatgggc tttatatgga ttcgcgttaa
     4321 gttatcgtta cacaaaaaat ccgtactatc tagctatagc agaaaagata gcagtaaatt
     4381 ttattcagaa aatcgaaaaa actggttata ttcctctcat tgattttgct gtaaaagatt
     4441 cgaatattga taccagtgct ggagtatgcg ctgcatgcgg tcttctggaa ttagcaggat
     4501 atgtttcctc tgtacgatca aaaatgtatc atgaagctgc tatcaatata ctccaaaaga
     4561 cagaaaagca ctatgcaaat tggaatatgg atgaagatgg gatcattgga ggcggagcag
     4621 aagcatacca tagagaactg acttataatg tgccactgat ctattctgat tattttttta
     4681 ttgaaggctt acttcgatta gaacagtcgg aattagctat ttggtagtaa aaagaggaga
     4741 aaatacaatg aaacatccag taccactatt aaggaccaga caggattact ttgacttatt
     4801 taaaaagata aatcaaccac tccagccgtt ttatcgagca agtggcgcac aaattgatta
     4861 tatacatcaa ggggtaggat atggaaataa aattgcagga atggaaggat tctctcgggt
     4921 tttatgggga gcggggcccg caattgacca attagatgaa cgatggttga aattaattct
     4981 atcaggaata aaagctggaa cagatccttc ccatcccgat tattggggag aaatacatga
     5041 aagagaccag cgtatggtag aaatgcccgc aatgttatta gcgttatatc atcatgaaca
     5101 attattatgg aaaaaattag atacaggaga aaaaagccag attattcatt ggttctcgca
     5161 aatttttgaa tatgaatgtg cagatggtaa ttggcaattt tttaagataa ttgtcggaac
     5221 aattatccaa aaatttggtt atccagttaa agaatcagca ctgaaagaag catttgataa
     5281 tatagaaaat tgttatcttc aaaatggctg gtatcgagac agttcaagag gtcgccaaga
     5341 ttattataat ccttttgcat ttcattatta tggtttgatt tatagcgtac tagaaccaga
     5401 aaagcccatt agccagatat ataagaaccg agctattgat ttttctagag actatattca
     5461 tttttttgcg gaagatggtg caaatgtgcc ttttggtcga agcatgattt atcgttttgc
     5521 agtttcaagc ttttggtcag ctatgttgtg ggccgatctt ttgcctttta aattaggtga
     5581 aatcaagggg ttagtcaatc ggaatataag atggtggatg caaaaagaaa tatttgatga
     5641 aaaaggtatc ctgaatattg gttataccta ccctcaattg acattgactg aaccctataa
     5701 tagtacgtta tctccattat ggctgaataa gattttttta ttgttggcgc tacctgaaga
     5761 ccatgagtat tggacggcta aagaaatgcc tatgcctatt cgtgaaggaa ctaaacttct
     5821 ttcagaagcc aatttgctca tacaacatga taatggacat acattttttt tgaatgcggg
     5881 acaattaggg ccaaatttcc atacattgac caatgaaaaa tatttaaaat ttgcttattc
     5941 tagtcaattt ggtttttcga taccgcgagc gaatcaattg aaagcagaaa tggcaatgga
     6001 ttcaatgcta gggattcagc gagcggacac atatatcacg accagtttta agaatgagag
     6061 catcaaaacc tatggacagt tcatcgttcg aaataatgta cgagacattg tatgtaagaa
     6121 agaggtactt gcctctacat gggaacctac tttacaggca aaaatcagaa cttggcttat
     6181 tccttttgaa ggctggcaga ttcgcgtaca caaagtagaa ttagatacag aatatgttct
     6241 atatgagaca gggtttgcta ttgaatgttc tcctgagaaa gaaggaataa tcatagaaga
     6301 agacaaggaa aactataggg tgagtgaagc tggcttttca ggtattatct gtttaagtga
     6361 cgatactatt aaacgaaaat ccacagctat tatgggtttg cccaatacaa acttgatgac
     6421 atgggaaaac acttttattc ctggattaga gggtacattt ggaaaaggaa cccattggct
     6481 cggaacagga gtggttgctc atcaagatcg ggattatagt ttagaagtat ggaacaatcg
     6541 tcccaaaatt gattttgatg ggacaaccgg gctaactatc caaaataaaa acaataaaaa
     6601 aagactaaaa ttatgctaaa ggctggtggg gaaatgcttg ataaagtgat gtttgaagag
     6661 ataatacatc cagaaaaatg gataaatgaa tgttataaag aattaatgga aaaagaatgg
     6721 aataatgaga tcgagcgttg tcgcagacac acggagaggt taatcaaacg tatcagtaac
     6781 agtcagtatc aattcccaag cgagtgtcct ataaaaggac gttatccatt aagagacaat
     6841 attggttgga cagaaggatt ttggacagga attaattggt tgttatttga gaagacgggt
     6901 gatcctttat ttcaacaaac agctgaaaaa gcagatatag attttatcca caggattgac
     6961 tgtcaaatta tcactaatca tcacgatctt ggttttttgt attctttatc aaccgtagca
     7021 gattataaaa taacgggaaa tcgaaaaatg aaggaagctt caattgatgc tgcagaaaca
     7081 ttacttcagc gtttttggga gaaggcagga gttatacaag cttggggaga tcctagtgat
     7141 ccttcacaac aaggtagaat gatcatggat tgttgcatga atcttccatt attgtattgg
     7201 gcgagtgaag aaactggaaa tcaaaaatat aaaaaagctg ctcaggctca tattcaaaat
     7261 gcagcagatt atttagtaag agaagatggt tcaacttatc atacatttta tatggatgtc
     7321 aaagatggaa aaccaagata tggaagtacg catcaaggtt atagtgataa ttcctgttgg
     7381 gctagaggcc aagcatgggg gatttacggt tttgcattaa gttatcgtta tttgaaagaa
     7441 caaaaatatt ttgatttaag caaaatatta gccaattatt ttatgaatcg gttgccagaa
     7501 gataccattt gttattggga tttgatcttt gatgattcta gcagagaaga aaaagataca
     7561 tctagtatgg tgatcatggt ttgtggatta ttagaattaa tagaatcagg tctattagac
     7621 aaaacagaaa agaacttata ccagtctgct gtttggaaaa ttatgcgaca attgaaagaa
     7681 agatatgcta caacggaaga agaagatggc tttttaacac agtctgttta taataagccc
     7741 aaaaataatg gggtaaatac accttgtatt tggggtgatt actattattt agaagcttta
     7801 gtgagaatga ctagtaattg gtcttcttat tggtgaaaaa ggtaaatatg gaatttttgt
     7861 ttgataaaat aaaaaaagaa aaaccaaaac agcatgaatg gtttctgttt tggttttttc
     7921 gttaattttc atattcttct aattccaagc ttagttcttc ccattccgtc aattttgttt
     7981 cttgatcttg tctagcagct tctaaatctt ggttcagctc attgagtttt acgtgatctt
     8041 ccaggttttc tggcgcgacc atttcttgtt ccaactgatc gatccattca tcgatccgcg
     8101 ccatttcttc ttcaacctga gtaatttttc gttgaaggct acggataagc ttttgctgtt
     8161 ctttgctttg gtaataatca gatttgttcg tgggagcagg agcagattct ggctcatctt
     8221 gtgccaacaa ttcggcgatt tcttcttctt cctgcttttt ctctaaataa taatcatagt
     8281 ctcctagata gactttgctg ccattttcag agagttcgac gacttttgtc gcgatccgat
     8341 tgatgaaata tcggtcatgg gaaacaaaaa gcagtgtgcc gtcataatcg atcaatgcat
     8401 tttctaatac ttctttgttg tcaatatcta aatggtttgt tggttcatcc aaaatcaaaa
     8461 aattttcttt gttcatcgct aacttggcta aagcaacccg tgctttttca ccgccactta
     8521 gcagagggat tggtttttct acatcttcgc cagaaaataa gaaagcacct aaagcacttc
     8581 ggatctcttt ttctggagtg gtaggatgct catcccacaa ttctgctagg atcgtcttgt
     8641 ttgtatgcag atcagcttga ccttgatcgt aatagccgat ggtgacattt gtccctagcg
     8701 tttcttttcc acggataaaa gggatctggt gaatgattga tttcagcaac gtcgatttcc
     8761 cgataccatt cggacctact agagcaatcg cttcttgacg acggatatct agattgattg
     8821 gttcagataa ggtgacatta tcatatccga tagcggcatc ttcgatttgc aagactacat
     8881 ttccagaagg cttttctata ctgaatagaa agtttgcttg tttttcatcc ccttgaggac
     8941 gatccagccg gtccattttt tctaatgtct ttctgcggct ttgcgctcgt tttgtggtag
     9001 aggcgcgaac aaggttccga gcaacgaagt cttctaattt atggatttct gtttgctgct
     9061 tttcataagc cttccattca cttgctaact gttctgcttt caaatctaga tatttcgaat
     9121 agttaccttt gtaatggcgc atttttcttc tgcttaattc atagacttca ttgactactt
     9181 tatctaaaaa ataacggtca tgggaaacaa tcaataatgc accagtatag ctttgcagat
     9241 aattctcaag ccaggaaagt gtctcgatat ctaaatggtt cgttggttca tctagaatca
     9301 aaatgtcagg gtgttgcaaa agcatgcgag ctaaagctaa acgtgtgcgc tgtccgcctg
     9361 ataaagtatc gattttctga tcataaaatg aagaatcgaa tttaaaccca tgcaatacgg
     9421 aacgaatttc attttcataa ccatagccgt ttcgatctga aaaatcgtgt tgcagccggt
     9481 cgtattcttt taaataagaa ctataagcag gtgtgtctgg cgtaccttcg ctaatgaaaa
     9541 tctctagctc gcgcatccgc tcttccattt gtctaacttg ttcaaaagct ttcagcatct
     9601 cgttccaaac tgtttcattg gaatcaagtc ctgtgtcctg agcgaggtat ccgagagtcg
     9661 ctgttttatt tttagcgatc tttccttcat caggtgcttc aattccagca atgatcttta
     9721 ataaagtcga ttttccagcg ccatttctgc caactaaagc gacacgagcg cctgtactga
     9781 tttctaaatg tatgttttca aataagactt ctgcaccaaa atggcgagca atctgattgg
     9841 cttgtaataa tatcatggaa tcctcttttc tactttgtga attctcctca aataaaaaac
     9901 tatttttcaa ttagtagtct accataaatt ccattatact gcgagatttg cagaaatgat
     9961 aaataaatga ataaagaata aaatttcaca atatctgagc aatggaattg attttttcct
    10021 ataaatactg ctatgatgga gaaatcaaag atgtaattag acaatacgtg gaggtcattt
    10081 cgtgaaagat catattattc caaaggcgac agctaaaaga ctgcctttgt attatcgcta
    10141 tttaaggatt ttaaataatg ctggaaagaa aaaagtgtct tcaactgaat taagtgaagc
    10201 cgttcaagta gatagtgcaa cgatccgtcg agatttttct tattttggcg aattaggaaa
    10261 acgtgggtat ggttatgatg tagaagatct tatgcacttt tttgcaaaga cattaaacga
    10321 cgatcaattg acaaatgttg cgctgatcgg tgtagggaac ttgggaagtg ctttactaaa
    10381 atacaaattc catcaaagta atagcatacg tatcagttgt gcttttgatg tgaatgagga
    10441 aatcgttggg cgcatcgtag acggtatccc cgtttatcca atggaagata tgtcagaaca
    10501 aatcaagatc caacaaatcg aagtagcaat tttaacgata cccgctgaaa gagcacaaga
    10561 agtagtcaat cagttaacgg aagcaggaat caaaggaatc ttgaacttta ccgctgctcg
    10621 attgaatgct ccgtcagatg tattgatcca aaatgttgat ttgacgaatg aattgcaaac
    10681 cttgatctat ttcttgcatt ctgacgcaaa tgcgcattcc gaaaatacag cagaataaaa
    10741 ataaaaagcg attgaacaaa tgaaggcaaa actttcactt gttcaatcgc ttttttctgt
    10801 ttggacaacg attattttgt tcggaagatt tcttcataag aaagggaatc atcttctgct
    10861 gtcagttcat ctagtagata acggattttt ttaaagtcga tgaaccgtgc ttgccgaaag
    10921 acttcttttc cttgatgaaa aatcaggaca actggcgcag tcagcacttc aaatttacta
    10981 gctacttctg gtgtttcaaa tgcatccaaa tgaagtgccg gaatatcata atgtgtgaga
    11041 agttcctcta gtcgtggacg aacagcgtga caaacactgc aattaggcat cgaaagataa
    11101 atgacggcaa cggatgactg ttgcagtatg tcttgaactt cagtcaagtt agtggtttgt
    11161 ttcattttta ttctcccttt tatgatagaa gtgatatgta tgagacctta atttaatggg
    11221 aataacagaa aaagtcaatt gaagtgcttt ggtaaaaaat aagagttgtg taaaaattta
    11281 cagagcaact tgtttttttt gaataaaagc gtatatttgc aaagcgaatg aactagaaat
    11341 aaaaaggagg aaactcatgt tttcttttat tccaaattcg gttttgcaga tgagtacgat
    11401 tttcttatcg atcgttattg aagcattacc tttcgtatta ttaggctgtc ttatctcagg
    11461 cgccttacaa gtatttttga ccccagaacg tgtgaagaga tggcttcctg aaaatcgtat
    11521 cttctctatt tttactggtt cgatcttagg atttttcttt ccttcatgtg aatgcggcat
    11581 cgtaccgatc gttcatcaat ttgttaaaaa aggcgtacca gttcacacag cgtttgcttt
    11641 tatgctgact gctccgatca tcaatccaat cgtgattttt tccactttta tcgcatttgg
    11701 caattcttgg cagatggcaa gctggcgggt gataggcagc ttttttgttg cccttgtgat
    11761 tggtgtctgg cttgcctatt ttcaaaaaga cgctgtattg aaagaaaaaa tcacgtctaa
    11821 agtagcagaa aatcaccatc atcatgtcca tgaagaagaa caaagtgaga agaatgaacc
    11881 aaaaaatcag ctgatccatc aaactgggca tgtgttgaca cattcaatcg acgaattttt
    11941 tgatacaggt cgttatttga tcattggcgg attaatcgca gctgcgatgc agacttattt
    12001 accaactaga attatgctaa cgctcggctc aacaaagatt ttagccatta tcatcatgct
    12061 gttattggcc tttacgatgt ctctttgctc agaagcagat gcgtttatag gatcttcttt
    12121 gttaagtctg ttcggcacag cacctgtagc tgcctttttg gtctttggcc caatggtcga
    12181 tatcaaaaat ctactgatga tgaaacgata ttttacagga cgctttattt tatcgctgat
    12241 cggactgatt gcccttagcg ttattggatt cacattattt atttaggagg gaaaatatgc
    12301 tgcggttttt gattttatct gggtatttcg aattgatgat gtacttgcaa gtttctggca
    12361 aactcgatca atatatcaat gtccattatc gctacttagc tattctttca atgattttat
    12421 ctgctctttt ggctttagtt cagctgtatt tgtgggtcaa aaacggaaaa gcagaagaaa
    12481 aggcacacga gcacgatcat gaccatgggt tgtcaaaacc ttatcaacgt ggaatcgctt
    12541 atgtactgct agctttgcca gtaatcgtgg ggacgttgtt tccaacagtt agtctcgaca
    12601 ctacgatcgt agaagccaaa ggatttaatt tcccagtaag taaagaatca gtaggggatc
    12661 cggagatgca gacacaatac ttaaagccgg ataccagtat ttatttcaac aaatcagact
    12721 acttagacca aatgaatgag ttgaaagaaa agtatgggga taagcaaact atcaagatca
    12781 cagatgaaaa ctatttagaa gttatggagc taatttataa ttaccctagc gaattcattg
    12841 gcaaaaaaat cagctatgaa ggatttgttt accaaacacc gaacgatagc aatacagata
    12901 tcttcttgtt ccgattcggc attatccatt gtgtggcaga ttcaggagtc tttggtttgt
    12961 tgacgcatat gccggaagga gtaacggtta aaaatgatga atggtataaa atagaaggaa
    13021 ccatccaatc agactattat caaccattca agcgtgaaat cccatctgta gttgttacaa
    13081 acgctgagaa agtctctgct ccgaaaaatc agtatgtgta tcgttctttt taaaagaaca
    13141 aaataaataa acatagctca gacagctgac acgcaaggac gtcggttgtc tggctttttt
    13201 tgcatgatgt attcatttgt ttgatattta ttttagaagc agcatcctga aagtatttga
    13261 tatcggtttc aaaggtgttg gaaaagattg tgaagagaat tacaatttat tcctaacatg
    13321 tatgttgaaa agttgtatga aatgattttg atagtaaaat atcgagtgga ggtacttttt
    13381 atggtaacgc gtatatttat tttggatgat agtttggttt ttgaaaaaat gctagtagaa
    13441 attttggaag aatccagaat tttattaagt ccttggaaat ttgagatcgt tcagggattg
    13501 aacgttatac aatttgttga attcattaaa aataatgaaa tctattcctc agatattttt
    13561 ttcctagaca tcgatttgaa cgcctattat agcggtatcg aactaggcga aatgcttaga
    13621 gaaaagaatg aaaagtgtaa aattgtttat ctaacgtctc atccgaataa agcaataact
    13681 gttattaatc gaaagattac gcctagtggt tatttgataa aagaattcac ttatgaaaaa
    13741 aataaaaaaa gcatccaaag ttacttattg aaagaaaaag aaaactatcc tgttcaacat
    13801 gctacgaaaa aaaggtggac aactgtccgt tatggtaata gcgatcacta tatccattac
    13861 gatgaaatcc tttatctaat gagcatgcca ggttataaaa ataaaatagg cctagtattg
    13921 aaagatgaag agattctcgt aaacggcaca attaaaaaat ataaaaaatc actcgaatat
    13981 gagtatttat gtaaagattt aaaagcttat atcattaata ctgaaaacat tacatctatt
    14041 agcagagtaa acgaaatagt aacatttgca aatggagaac aactagagat gggaaagaga
    14101 agtattgata aactaaagaa ttttatgcaa gaaaagatag aaaatgaatc attttaaaga
    14161 aaacaaagac tcatatctta cctgttagaa gagtgttttc ctgtagaaga gaacaaccca
    14221 agtagcagcc tagtcgtttc tatccttttt tgttatgcac tctttttgac atttactcca
    14281 ccaataacga agggtacaat aaaaagtaat aacaaacaaa aggagtacag aaatgattga
    14341 attcatcaat gtaagtaaaa gttacgggaa aaaacaagca ttaaaagact tgtctctctc
    14401 gataagacaa ggggaaattt ttggattttt aggacataat ggtgccggaa aatcaactac
    14461 gattaaaagt ttagttagta ttattgaacc atcatcggga acgattttag tagatggaat
    14521 gaaattgacg gaaaatcgat tgagtatcaa acaaaaaatc ggttatgtgc ctgattcccc
    14581 agatattttt ttacaattga cggctggaga atattgggac ttgatttcag ctgcctatga
    14641 attagacaca caaaaaaaag aaaagcgttt agcagaattg acagcgcttt ttgatatgta
    14701 tagtcatcaa aatgaaacaa tcgcaagttt ttcacatgga atgcgtcaaa aaacgatttt
    14761 gatcggcacg ttgctgccag atccagatat ttgggtattg gatgaacctt tgcaaggact
    14821 tgatcctcaa gcagcatttg atttgaaaga aatgatgaag gctcatgccg caaaaggaaa
    14881 aacagtcatt ttttctacac atgtattgga taccgcacaa cagttatgcg ataaactggc
    14941 tattttgaag aaaggtgaat tgatttatca gggggcagtt agtgaattat tgaatggctc
    15001 acctgatgaa acgctagaaa agatttattt gaaaatggct ggaaggcaag cgacaggaga
    15061 tggaccgtat gtataagcga caattattag aattaattcg agttaatctt ttatatgtta
    15121 atccacagct cacgaataaa gcaagagaga aaggaaaaaa gggaaagcga ttaactatct
    15181 atcttttgtc tcaaaatctt ttttcagggc tgattttttt actgatttac ggatttgcta
    15241 tgtttgctat gaactttgtt aaattgccag gtttctttac ttattacgtg gcactttttg
    15301 gtatgttagg tttaactcaa agtatatcag tcatttataa tatctttttt gaaggaaatg
    15361 acctgcaagt atttttgcct ctgccttttg gacaagaaca ggttttttca gcaaagattt
    15421 tggtagttgc tttgaccatc attccttttg tttttccgct tctagtcatg tttattttaa
    15481 ccggatggcg agctgatcta gggattttgc ttactatcat tatctcaatc cttttgtttt
    15541 ctctattttt gattattctt ttttgtctgg ctagtttgat tgtttttggt ttggctcgaa
    15601 cagcagtgtt taaaaaacat agaaaaacag tttctagtat cttgcttggc agctcgatgc
    15661 tgatcgcatt tgctggaatc atgtggatga atcatcaaac ggggactatt gaaaatgagt
    15721 taacagatcg gcatccgatt tcgatattat tgccattcta tgatgctgcc agtcagccac
    15781 tttctcaaca aggtttattg ggttggggaa tactagctat cacggctctg ggatttttcc
    15841 ttgctgtaaa acagctgata gtaccaaagc ttacagagca actgactgat tcaaacagtg
    15901 agttcaaaat aaaaagaacc cataaaaaga accaaaatgt gcatcagctt ttatttagtt
    15961 acaatgctca attagtaaaa gaacctaatt tgattatgca agttctttct tcttccttgt
    16021 taactccaat catttttatt cttgcttttg ccctaggagg cgaaataaaa ttaactgatt
    16081 tagataataa gttaatcggt gttgttttcc tggtagggat cgcattggct ttcttaacag
    16141 ttaatcagac ttcttttatc agcaatctta tttctttgga tcaagaaaat tttctgttta
    16201 tccgttcttt gccaatctct atgaaccaat atttaaaaga aaagttcaga ttcggatgga
    16261 tattgcagag ttttttaact ggtgggatcg cattattaag cggactgtct tttcagctgc
    16321 ctttgttttt tatccctagc ttgctatttg gttctttatt cggctgctac ttattgtcac
    16381 tacgttattt cgctagagat tatcggttgc tgctgctgaa ctggaccaat attaaccaat
    16441 tatttaacag aggttcagga agtttgggtt tagtcgcaac tatgatgggt tcattgatca
    16501 tcagtattat tcttatcgtc ttatacggaa tgttggcttt gttttatcca ttttggttag
    16561 tgaatattcc tgtgatcggt ttagttctaa tactttccgc tctatggatc gtttattatc
    16621 aacgaacgtt ttggaaaaaa tttgaataaa gaagaggggc cgtgacaaaa atcatgtcgc
    16681 agcccctttt ttacagatag agggtgttca aaagtcagtt tctcagtatt aagtcaattt
    16741 tatgtaaaaa tgtgagaagc tatttttaca aaactttttt taatactcat aattacttct
    16801 tattgctcct gcggttactt gtcgcaatgt ttgtgagata ttttgtcatc gctcaaggct
    16861 gaacgtctct ttcacaacct tttctttgtt acacttcgtt tatttaaaaa gccgtttttt
    16921 tcgtttcatc atgattgcgt cacgctttac tttgaaatta cggatgttat ggttaaagac
    16981 ttctgataat acaagaccta aagcgattgc tccagcaatc ataattgctt ggaccgcata
    17041 atgagccgct tctcgatagg attcagtaac gagatttcga accgcttgat aagctaaacc
    17101 accagggact agaggaacca tcccgggaat attgaaaatc gttacgggca ttttcaaacg
    17161 tttagagaaa agatcgctga tgaatgctac tcctaaagaa ccaagcaatg aaccaaaagc
    17221 agcagatccg cctatttgaa cacagatcca gtagatcatc cagccaaaag caccagaaag
    17281 accacaacaa atcaatgaac gcctaggtac atttgtaata acggcaaatg ccgcagtcgc
    17341 taaaaaacta aaagaaaact gaataaatat cgtccccata cttgtgttcc tttcctaata
    17401 aaaaagctgg aaaataaaag caatcgcaaa accgatcatc gtagcagtca tcatcgcttc
    17461 tgttccgcga gaaactcctg agacataatg tccagctagc aaatcacgga tagcgttcgt
    17521 aatttgaact ccaggaacta aaggcatcac acatccgata atgattagat cctgattgct
    17581 tcctaagcca aaacgtacgc tgtagatagc ggctatgcca atcaataaag aagataaaaa
    17641 ttccgataaa aatttgattc taaaaaattt caaactatat agataagctg cgtatcccaa
    17701 tcctccaatg actaaagtca caggtaaatc ggtcatcttt ccgccaaata acaccataat
    17761 tgttccactg atcactgccg cgctaacgat ttgcaaccag acaggaaaga aagaacggtc
    17821 tcggtcgatt gcttgcagtt cgcagtaaag ttcttctaat gttagctctg aagcaacata
    17881 tttacgtgaa agatcattta cttttactac tttttctaaa ttaatcgaac ggttggtaat
    17941 ttgttccatt cggatggtcg aggtacgatc aagaccaata aagagaccgg tttgagtgac
    18001 atagctgacc aaacggaaat ttccagaagc taaagcaatt cgactcatcg tatcttctac
    18061 ccgatacatc tccgcattgc tttccatcat gattttccca gcgagtaaac aagtgtccag
    18121 caacagatct aattcttttc ccgtcatagc atcttctcca ataattgatt gattagtttt
    18181 attttactac aaaacatacc agaagcgtaa agaataaaaa aacttgagga aggaataaaa
    18241 gaggcgtata ctgtttttgt attgcgggtg tagtttagtg gtaaaatccc agcttcccaa
    18301 gctgatgtcg cgggttcgat tcccgtcacc cgcttaaaat aggttgtgaa aaagctgctc
    18361 tgcaacaagt aataagaaca gccaagcaaa aacaacttct tatgttttta acttggatgg
    18421 acttattacc gcagatgcaa gcttttgcgc accgtttacc aagcggttgt gaaatcagtg
    18481 tattgtcttg agtatgaaga aaatttttga ataataataa ataccttaca gatagagtgg
    18541 tgactgatta gagacgagga atccgtctct tttttgtatt tttaagccat cttaaaacag
    18601 gttttatttt ttgctgtttc tatttctttc tagtatttat ttatagggga acatatgtta
    18661 agatttaggg agctgttagt aaaggagtaa agaaatgagc aaaaaaacaa ccaaggcgat
    18721 cgtggggatc gtcgctgctg tgattgcagg ggcattcggt gtttccacca cacaaaaaga
    18781 ttcagatatc tttcagcaga ttacaactat ttttgattcc acaccacaag cttctcaagt
    18841 agcggatgtg ccagaatatg atggaaaaca tcaagaaata gaaatcaatg ggaatcagcc
    18901 ggaatttaca aaagaggatc ttagcctttc caaaggaaca tgggaaagct attcaaatat
    18961 tgataaattg aacagagtag gacaagccaa tgctatgctt ggaaaagatt tgttccctac
    19021 tgaaaagaga gagcctttgt atattgaccc gacaggttgg aaacaaaaga aattaagtga
    19081 cgggcaatgg ttatataatc gcagccattt aattggtttc cagctgactg gtcaaaataa
    19141 caacattaaa aacttgatga cagggacacg ttcgttgaat gctccataca tgcttgctca
    19201 cgaaaatgat attgcggcat atatcaaaga aacaaatcat catgtacgct accgtgtcac
    19261 tcctcatttt gaggggaatg aactggtagc caggggagtg caattggaag cagaaagcat
    19321 tgaagacaaa aaaattgaat tcaatgtctt tatctacaat gttcaagatg gctacacgat
    19381 caattatgag acaggacaag caacaaaaac aaaaaattag tagcagctat tcgaattttt
    19441 tatgaatatt atgaaaaaat tgtgtaaaaa catacattca aagaaatata gcgtatactt
    19501 tatgaaaaga gtacttttga taaagctaca gaaaaaagga aaaaaacata caaatagtgt
    19561 ttcttaattt attcggaagt ttggaatatt tgtttgtagt tttccctttc ttcgagtaaa
    19621 ataaggttgt agaaggtaag ctggaattac cttgcgggaa agaggtgtag aatcatgggt
    19681 tttacagatg aaaccgtacg ttttggtttt gacgatagtc gcaaaaaaga agtgagcgaa
    19741 acattagcga ttgtttatcg ggctttggaa gaaaaaggct acaatccaat caatcagatt
    19801 gtcggctact tgctttctgg agacccggcc tacattcctc gttatcgtga tgctcgaaat
    19861 ttgattcgcc gtcacgaacg cgatgagatc atggaagtaa ttgtcaagga ctacctctca
    19921 aatcatggag tgaatttatg agagtaatgg gcttggatgt cggttctaag acagtaggtg
    19981 ttgctatcag tgaccccttt ggctggacag ctcaaggagt agagatcatt cggatcaatg
    20041 aagcagaaga agagtttggc tttgatcggt tgaaggaatt agtagcacaa tatgaagtag
    20101 accggtttgt agttggatta cctaaaaata tgaacaattc catcggacca agggcagaag
    20161 cttccatgtc atacggagaa aagatcaaag aactattctc cttgccagtt gattatcaag
    20221 atgaacgatt aaccactgtt caggcagaac gtatgctagt agagcaggca aatacttcaa
    20281 gagccaaacg aaaaaaagtc atcgataaat tagcagctgt gatgatctta caaaattatt
    20341 tagacggctc aggtaagcaa ttattttaat aataaaagaa aagaggtaat cttatgacag
    20401 aacataacca ttcccacgac catgaaggac acgaacacat cacattggtg gatgaccaag
    20461 gaaatgaaac attatatgaa atcctactaa cagttgacgg acaagaagaa tttggacgta
    20521 actatgttct tttatatcct gctggtgttt cagaagatga agatgtagaa ttacaagctt
    20581 atgcatacat cgaaaacgaa gatggaacag aaggcgagtt agaacaaatc gaaacagacg
    20641 cagaatggga catgattgaa gaagtattca atacattcat ggcggaagaa gaagaataga
    20701 ctataaagct aggagaaacc ttgatatgat agggtttctc ctttttttat tttttactgt
    20761 atgccatatt gtatgccatc tagtcaatat ttaagtgttg tactaatttt tcaacagtct
    20821 tttctttcgt ttcttttgcc aagtgaccgt aacaatcgat cgtctgacta atttttgtat
    20881 gccgtaatct agtttgaact tctaccatag tagctccagc gtccaagagc attgaagcat
    20941 gagtgtgtct tgctacatgc acaccgagaa atggaaccat agctttttca caaatagtaa
    21001 tcaatcttgc ataaatagcg tggtgtgtga ttagttcttt atagatacca caaaaaatca
    21061 tagtcggatc agttacacca agtgtcagca tatatttccg ctgattaatc tgccattttt
    21121 ttaatataga aattgttctt tcatctagtg gaagaacccc agcactacaa tcagttttag
    21181 gatcactgat tttatagcca tttttggatt ggcttagtgt tttattgatg gtgaccgtat
    21241 tttcttgaaa atcaatgtct gaccaattca atgcaagggc ttcactgatc ctaaccccgc
    21301 tataaaagag aaagcgtagt aacgttttat caaagtcgtt tcgataagtt tcaggtttgc
    21361 tgtttaggta atcaaacaag gtgatcagtt gttctttact gtaaactttg accttttctt
    21421 tatgcttagt ggtggttctt ttaggcatta atacgttgtc tagtggatta gtctcaataa
    21481 tcccaagatt gatagcgtag ctaaaaatac gcttagaagt tgaatgaagt accttgtatt
    21541 ggtcagtagt ttcagcccag ctatttacaa tttgttgggc tttttttacc gtgacttcat
    21601 tcacacgaag atttccatat tttggaagaa tccactttct aaacttgatt tcagttttca
    21661 tgaaggtggc ttctcggaca gtggttttat atgtttctag ccacaagtca taaacttctt
    21721 taaacgtttc gttgttcgct tttttaatac cgtttttatc gtagtccact tgcaagcgtg
    21781 acaaggcaag ctgggcttct ttttttgttt tgtaacctcg tctacgaacc ttatcaggtt
    21841 tgccagtcat tttgttcatt ccaagatagg cttcatagag ccaaaattta ccttttttag
    21901 tttcatattg cttgaatgtt gccataatag ttttccctcc atagtgtagc aagccgtggg
    21961 ggcggttgga ggaggcagtt tattattatt ttgaatagtg tttatttagc tcagtaacaa
    22021 gagctgtgaa ctcttttaga agatcatttt cataaaattc ttgatactct tttttctcat
    22081 ctgtattttt ttctgaatca ttaattaatg agtcatatcg taaaagcgta tttagaagag
    22141 ctgcgagata agcaattggg gtactcccgt caggaaattc aattcgagat cctgttttaa
    22201 gatattttat tgcgtttgtt aaataaatct tctgtgagac agtcaggtta tcttcttcga
    22261 tgttattgat agcattagaa agattagaat caattataga gttatttttt tcaatagttt
    22321 ttttcaattg cttttcaagc acatttcgct taatttcggg catcatatgc atatctctta
    22381 atgtataaga tccgtttaat agataggtag tggatacatc taataccttt gaaagttgtt
    22441 taagcctggt gttattggga agattatagc cattttccca attactaacg gcacctttac
    22501 tggccggtgg ggataattta cctccaagtt gttccattga gagtcccttt tcaattctag
    22561 ccagtctaat tctttgtcct gtctccactg cttcggggta tttttttcta gccacatttt
    22621 caccaccatt catatatttt ctctactata tcacgattta aatatgtgtg aaacataaaa
    22681 gttcaaaaaa gtatgttaaa attcttgcaa atgaaaaggt atgatggtat attgagttta
    22741 taagaggtat agaaatgtat tcaagagcct cttgcatttt tttaaaatct ttgtatagaa
    22801 atgtatgcta acgagggggg ataaatatgg aattaaaaat tgacaaactc acgcttgatg
    22861 atccagcttt taaaattatt gacgatcgag ttgttcaaca agtagagcaa actcaaggaa
    22921 cgttactttc attattagaa aaaaagtacc agtttccaaa atatatgaac aaggcacaag
    22981 ctgctaagta tatgaacgtt tcatacaata ctatgatgca gaaatatgtt cctaacggtt
    23041 taaagttgat tatagttgat ggagtcgtaa gaatttcaca agatgaatgt gatcgcttta
    23101 tggaagaata ccaaaaataa acgcaagccg tgggggcaaa gggattaata ggaggaaata
    23161 ataatggaat cgacacaaaa taaagcaatc gaaaaagtat tatcaacaat tcttacagaa
    23221 gacacagcgg cagaattagc aaacttggaa ggcaaagcgc tagaagaaac gtttgaatgt
    23281 ttgtacgaac aaatggacta tcaaaaattg ctaccacaag aaccaacggt aagcggagta
    23341 ttgcgtggct tgaacgattt agtacaagca gagtttaaag aacgactttc tattgaagag
    23401 tatcaagaaa ttttgtatca gcaagttgag ttgttagcga gcttgctagg gcttgaactg
    23461 gaggacgttg aatgaaaact gaaatttgga acggacacat tataagattt gtcgatatta
    23521 atgatgaatg gtgggcagtg gcgaaagatg ttgcggaagc gttaggtctt aaacaggtta
    23581 ctagagccat tcactctttg cctaaagatg gggttactac tagtaaggtc atcgattcat
    23641 tgggcagaac acaagatgta aacatcatca acgaaaaaaa atatttaccg catggcattc
    23701 aaaagccgta aaaaagaagc cgaggcattt caagactgga ttttcgacat catcaaagaa
    23761 ctacgccagt ccacaggact tgaaggcttt caagtattcc gcatgctaga caaagaacac
    23821 caaaaagaag caatgactaa attaagccac gctattactg aaccaaaacc agtagactac
    23881 attaaagcga atgtgatagc caataaagct gtatcaacta tttatggtca ttccaaaatg
    23941 gtaaaaaaga aagatatgac tcctgaaatg ttggttgatc gtgaacctat tttagatgag
    24001 acagtagaat taatgactgt taaagaaaaa tatgggttgc agttttcagt gagtgagaaa
    24061 atttataatc gttctgctga attgcagaca acttaggagc attaacatga tcgagaacaa
    24121 agaaaaagcc ttatccataa cagtcttggc ggacaggata aaggcaactt aattgggata
    24181 aaacccgata ttctttgttc cgattatacc atattaaaaa atcggaggga accattatga
    24241 aacgaaactt aattatagga agcattttaa tcggcggaat tttcggctta ttaccattaa
    24301 ttgcaaaaat agctatcttt gcaggattag cgttatatct cttcactgct tatgacgagt
    24361 acgactatca attaagaact ggagggcata agtagatgta caaaacaaaa ctattaaacc
    24421 aactcgatag ccttgaatta gaggaaatta atcaagggat cgctgaacta gaaaataata
    24481 tcgggaaaac ctactttgga aattcattca atgaaaaact aacagttttg tatgtactca
    24541 aaaaacatgc agaacacaaa ataatttgtc gagaaatcaa cgagctaaaa aatcagattt
    24601 taaccgcatg gttaaacatt actgatatgc aagaagcaag ggtgaaaaca tttaatacat
    24661 gggtaaagta tcagaatcaa ttaaaaggtg ctgaatttgt tcgggatggg ttgaaatacg
    24721 aacttgagca gttgaagctc atggaggtgt ctgaatgatt tataccagta ttttgaaaaa
    24781 ggaggagagg tttattggca aataattatc aagcagcaac ggaattacaa aagtcgggat
    24841 ggtcaattta tcccttatct cctggaacta aaattccaat aaaaggctct agtggttgga
    24901 atgatgcaac taacgattct aatcaaatta atgaatggtg gacggaagat gcaactagaa
    24961 atattgggtt aatgcttgaa gatcatcaaa tggtggtagt tgacctagat caacattctg
    25021 aaacacgaaa cggcgttgag aattataaaa aacttgcttc aatgtatgat ccatttcctt
    25081 ctacttatac ggaaatcaca ccaggtaaag gaatccattt cttctttaaa attcctgatg
    25141 gtgtaattat ccaacaagaa acaggtgcat ttgctgatat cttcgggcgt gatgataaag
    25201 gtaaaagttt aagcgggatt gatattatca ctaaaggagt tccaatagcg ccaactatga
    25261 ttatttcaag aagtgttggg agagcatatg aacctattga aaacgtatct ctaaataata
    25321 ttgcaccagc tcccgattgg ctagtgaacc tactgctaaa aaaaccgcaa gaaataaaga
    25381 ctcaataccg acccgtagca aaaacaggga cagcaaaaaa attagatatg attgttcaag
    25441 gcgctggcga aggtagtaga aacgatcatt taactaagtt atgcggttgg ttactatggc
    25501 acggtgtaga taatcaaact ttagcagaat tgatttatac agcaaatgcg tacaatgaac
    25561 caccgctaaa agataaagaa gtgaatcaaa ttattcgatc gatgattaaa aaagatttga
    25621 gggggaaagc tcatggctca taagcataaa ttagatagta ttttagactt tcccgaagcc
    25681 agcgagcgtg aagacaatat cattgaattg aaaacctgga tgtcacgatt aagatgcaat
    25741 aaagacgatc agattaaaag taacagtgtt gtgaatgcag aattgatttt aaccaatgat
    25801 agcaatttag ctggaacaat tgcttataac gaatttagcg gatacattca cctattaaaa
    25861 gattctcctt ggatcaatcg taccgctgga gaatgggaag acagttttga ggatgcctta
    25921 acggcatata ttgaagaaaa ctacaatgta gtttttgatg acaacaaaat acacaaagca
    25981 gtagtaaatg tggctagaaa gaatgttttc aacccagtaa aagaacgtat cgaaaaagtt
    26041 aaatgggatc aacagcctag actggaaaca ctgtttattg accttttagg ggtagacgac
    26101 aacctttaca cacgagaagt aacaaaacgt tggatagtcg gatcagtcgc acgtatctat
    26161 aaaccaggaa taaagtttga gatcgttcca gttttggatg gaccgcaagg gattggtaag
    26221 agtaccgttc cagcattact ttacacggat gattttttca ccgattcatt agattcgcta
    26281 ggtgaaaaga aagatgatta tatgcagctt caaggcaatg taattattga acttggagaa
    26341 ttatcttcaa tgaacaaaac taaaattgaa cagataaaaa acttcatcag tgccaagatc
    26401 gataaaatta gacctcctta tggtcggaat gtcatagctt gggcaaggca atgtgtattt
    26461 attggaacgt cgaatgatgg gcagtatttg aaagatgata caggaaaccg ccgtttttat
    26521 cctttgcctt gtaagaataa gccaaagatg aatccgttta aaacaaagga tgaatatttt
    26581 cttcaagtgt tagctgaagc aaaggtgctt tttgataaag gacaacgaat ctattttagt
    26641 cccgatgaag ataaagaagt cttagaaatt gcaaaagact atcaagagga tgccaagatg
    26701 gagaatccaa ttaaagaagc aatcactaaa tatttggaaa tggaaatccc gtataaatgg
    26761 gaagaagctc cagcgtgggc gagacgttct tattatcaga actatcctga cagtaaatgt
    26821 aatgagaaaa atttacagca tttctataat ggagctacta tgcaacaaga gttttattta
    26881 gttgatagcg tactcaccgc tgatattttg gaagctgttt ttgataaaca agcaaaggat
    26941 ttattgaatg gtcgatcaga tgcagaaact aaaaagattg ccttgattat tagcagtatt
    27001 cctggttggg aacggaagca actgccaaaa agaaataaac gacgaggatt ttacaataag
    27061 tcaaatgcct atgaaaataa aaatcgagct ggaaagtaca aaaagtaacg ggtagtaacg
    27121 ggtcagtaac acctagcccg ttactagttt acccttactg ccccaaggga tacagcaatt
    27181 tggtaacggg gtaacacctt atttccttaa aacttttgaa atagttagct aatatgctgg
    27241 ggaataatgt tactggattt tacccgttac ctgttaccaa aacgctagaa cccttgtccc
    27301 cgttgggatt gagtggtaac gggtacctgt tacgtaggtg ttaccacgtg ttacgaatca
    27361 aggaggaaag taaatgctca atattatttc aacaaacaaa gcaccgaact ttcaatatac
    27421 agatgaaatg gatcggtttc taatgaacac gctggcattt agtgtgggat tggtaacaga
    27481 agattattcc acatttgatc cagaagtatt aaagataatg gaagaagaac ctgattggct
    27541 gcaggagtca gtagcgtggt gtcaatcgct agtagtggga tcattggtag atagtggcaa
    27601 ctatgatgat acaggtgagc taatggatga atttaattgt ctgcttaacc tatatgatcg
    27661 agcgagacaa cgagagctta catcgaatga ggataatttg tttttaaaca tccatgataa
    27721 gttcttggct ttgctactaa cagatgatga attgataact aatttattgg aggtagaata
    27781 attatgcata tggaaagtat tagatacttc gatggtgaga aacacattga gtattcaaac
    27841 actcaagaag atgtagactt tattagtttt gaggaagata aaatcactaa agttaatttt
    27901 aaagacggaa cgtgcttgaa gatagtttcg ccttatattg aatacaaatc aaaatgggaa
    27961 caagatgatt ctgacgaacg agatcgcttt ggttggtaat ctatggcagt taaaaaacaa
    28021 tgtaatcatg ctggatgtaa aacattgatt gattataggc agaagtattg tgggaagcat
    28081 aaagcaaagc aaacggcaat taagcgtgaa gaaagaaagc aaagcgaagg taaatatttt
    28141 caattctatc aaagtagaac atggcgaaag gcttcatact tgtatcggtt aaatcatcca
    28201 gtatgtgagg attgcctaga agaaggcttg ataaggaaag ctgatgttgt agatcacaaa
    28261 attgaactaa aagatgattg gaatagacgg ttagatgaaa ctaattttcg ttctttatgc
    28321 catgcacacc ataattccaa gacagccaat gaaagacaaa gacgagaaaa gagtaccctt
    28381 tgagttaagg gggcatagtg tgaaacttac tgacaatcga tgcctactca ttttggtaca
    28441 aataaccgtt gtgaaaaggc ataagggtaa ttacaaataa cgattacact tgtaatcaca
    28501 agtgaaaaag tatataatga aagtaggaga tttattgaaa aatacaattg ttattactga
    28561 taaaacaggg aaacagcgca ccattgagtt acctcatttt ggtcaagtga ccattcaaat
    28621 gcagaacgga aaaatcattt atatagataa attagataaa gaaaaattct gatcgaaaaa
    28681 cgaaggaatg tgagcttaat tgcttacgtt cctttttctt ttgtctgaaa ggaggacaac
    28741 atgggacacc ctaaaatatt acacgataca aaaggcaata tatcaagtga agaaaaagca
    28801 atccgtgtgg acgctagaga agaattgttc aagcagcaac cattaataaa tatcacgccc
    28861 cctgactgga tggcagcgag cgctagaagt gaatggaatc gtatcgtacc aacattaaaa
    28921 aaagattatc cattgagtga agcagattat ggttcattgg tagcctattg tttagccttt
    28981 gctcgaatga aaacagccga agccgagata agaaaatcag gtacgtttat cacatgcgaa
    29041 aacggagtaa agaaagccaa tccagcggtt cgggttcaat cacaagctat gagtgatttg
    29101 aaaaaacaag ccacctcact aggtatgacc ttagaatcac gatcaaaact agctttgaac
    29161 aaggttaaaa atgatgaacc cgaagacccg ttcaaagagt tgatgaattc atgaatgatt
    29221 acattgagaa agtcctatca ggtaagctga ttgcacccaa gaaaattatc caagcgtgtg
    29281 agcgtcatat aagcgatttg gagcgttcta agtcagatag cttcccttat gtgttcgatg
    29341 aagaacaagc caccaaagcg attaaattca ttgagttgct accatctacg gacggtaaag
    29401 caatcaagat gttaggattt caaaaattca ttctaggcag tctttatggc tggcgtacta
    29461 aagaagggaa ttacaggcga ttcaatcgag cgtttaccag tatgagccgt aagaatggga
    29521 aaacgtatat cgcaagtggc atggctgcca atgcgttgat tatggaacaa gaaccagcag
    29581 aagcaaggca agtattgttt gtaagtaacg ctttgaaaca agctaaattg ggctatgata
    29641 tgctgtctaa ttcacttaga aacgtggtca agtctagtaa gtttttaaga ccacaactga
    29701 aaattatgaa ctctaagatt cagcacttgc cctctaattc gttcgctatg gcactggcta
    29761 gtgaaaccag cacgctagat gggtttgcac caacaaccgc aattcttgac gagtggcacg
    29821 aagcaaaaac tcgtaaaacg tacaacgtca ttaagtcagg aatgacccaa caaaagaacg
    29881 gcttattgtg tgttattagt accgctgggc ttgatttaaa cgttcctatg tacgaggaat
    29941 acttattgtt agatcgtgtg ctaaaaggcg aagaacaagc cgacaggtac tttatagcga
    30001 tatgggaatt ggacgatccc gaagaaattc acgatcaaga gaaatggatc aaagccaatc
    30061 cgatttttga aagtgaagaa atcaaaaaag taatgattcc aaccattcaa gatgatgtga
    30121 accttgcttt gaaacaaaat aaccttaatt ctgtattggt gaaaaacttc aatttatgga
    30181 gacaagcgag tgaggacagc tatatgattg ctgaagactg gcaggcaacc gaagtagaac
    30241 cacaagatat tacaggcaag cccgtttata tcggagtgga tttatctaaa acagatgatt
    30301 taactagcgt ttcatggatc gtaccgctag ataacggcga actttattgt gattcacata
    30361 gctttgtagc caccaaatat gggcttcaag acaaagaaaa gcgcgacggt ttgccttatc
    30421 gagaactaga aaaagctggt gagtgttcca ttactcaatt agaaagtgga atcgtggact
    30481 atgaccaagt atttcagttt attcaagatt tgattcaaga aaatgatttg gaatgtatgg
    30541 ggatttgtta tgacccgtat aacgctaatt cgcttatcag taaagctgaa aaagccaact
    30601 acccaatgtt agaagtaaga caaggaacga ttactctaaa cgttccgacc cgaactttta
    30661 gagaacaagt ttatgaaggc aacgttattc acaataaaaa tacgattctc acccatgcag
    30721 tgaacaacgc tattttaaaa acggataaca acggcattca gattaataaa tcaaagaaca
    30781 gtaacaaaat tgatccaata gctgcattaa tcaatgccta tgtgtttgca atggattact
    30841 tcaccacaac ggaaggagcg aaagcagaca atgaatttta tacaagtgaa gaattttctt
    30901 tctaattaca ttcataccgt tcttttactt cttggattgg tgtgtgtgtt ggttgctatc
    30961 accttactaa caaatgtcta ctatggcttg ttagcgctgg gcatagtgct tattggggta
    31021 gcggtcatgc taaatacaga acagaaggga ggttaaaaga tggcattttt taaagcgaga
    31081 caaaatacaa cgggagatcc tttcctacaa catgtggtat caatccaatc ggatgattac
    31141 accaccagct ttacaagcgt tcgtgcatta agaaatagtg atgtgtttgc agccgttcga
    31201 attattgcta gtgatattgc ttcaagtccg attcaattgg tcaaaaacaa tatgccacaa
    31261 gctgataatg aactggtgaa gttactaaac gagaaaccta attcagaaat ggatggttgg
    31321 catttaaagt ttgctttggc ggtcaatatg ttgctaaacg gtaatagctt tgcagagatt
    31381 aaacgcaacg gtgaaaaggt agaagaaatt cacttattac ctaactcaag tgtaacggtt
    31441 actcaattag ataatggtac gttgtcttat cagattggtg ataaaaaaag acgtgtgaag
    31501 tctagcgata ttttgcactt caaatatttc acccaagatg gtttaacagg attaccacca
    31561 ctttatgcgt tacgtgatga actgaaaatt caacaagctg gcaaccgcac attgcacaat
    31621 ttctttactc gtggtgtcag tgggtcaggt attttgaagg ttcataagtc agatttagac
    31681 ggatcagcaa agaacgcaat tcgtgaaaag tttgaagaag ccaacggttc aagtagtgga
    31741 gataatgctc ttagaacgat cattcttgat gaaacaatgg actacaaaac attagaaata
    31801 aacaccgatg ttttgaagct tatcaattcc aatgattgga acacaaaaca aattgccaag
    31861 gcgtttggtg taccgattga gcgtttaggc gttgaaaatg aacattctag caccgttcaa
    31921 agtaatctcc agtatatcca aagtacgctg attcattact ttaatgtgtt tgtgagtgaa
    31981 ttagatacga aacttgaaac caatatccgc tttaattccg atcagttact agagactgat
    32041 cctgaaacaa aagtaaaaaa tgtattggat caggtcaaag ggtcacttct cacgattaat
    32101 gaggggcggt cgaaaatggg gctacccccc atggatggtg gcgatcgttt actagcaagt
    32161 ttgaacttta cgtatttaga tacgttggag aaatatcaat taaaagaaca ggaaggagtt
    32221 acaccagttg aataatgaag aagaaaagga aaaacggctg acagaagaag ctgagctaac
    32281 agccgattct cccaaagtag agaaagaaaa tgaaaaacaa ccaacagacg gcaaaactat
    32341 ttcaggctat gcgttgaaat tcgggcaacc gtcaaaagat ttaggcggct ttgtagaagt
    32401 gattacaccc gaagcattaa aagaggtgga tttatcaaat gtgttcttat tatatggaca
    32461 cgattattca aagccactag ctagtgtcaa agcaggtacg ttgaaattga atgtcgatga
    32521 tacaggatta cattttgaag cagagctaac cgacacgacc tattcaaatg atgtgtatga
    32581 gaatatctca aaaggggttg ttgattctat gtcctttggg ttcgtgcttg gattagattc
    32641 attcgacaaa aaagaagatg gcacaattga acgatcaata gataaaatca aagcacttaa
    32701 tgaaattagt gtcgtgaccg ttcccgctta tgattcatca aatgtccaag taaataagcg
    32761 ttcttacgaa tcgtttatga gtaacaacca agcaaaacaa acaaacaata gcttagaatc
    32821 cacttctaaa gcacaaaagg agagtaataa catggaaaaa actttaatcg ataacgaaaa
    32881 aacagaattg cgtgggtatg aagaatatat ccgttcacaa ggcgaagtgc gtgatggagt
    32941 cactactgta aatgcagcgg cagttgttcc cgaagaagta atcggtgaag tctttgattt
    33001 gaaacgttca aattataact tagctcaata tgcaacagta aaaacagtat caaatggaca
    33061 aggtaaatat ccagtagcaa ctaaccaaca agcagtgtta gcaacaaaag ctgaacttgc
    33121 tgaaattggt gatattgacg ctgaaatgtt tacttcagtt gattataaag tagaaactcg
    33181 tgctggtaag attgccttat caaatgaggt tgtggaagat tcagcagtga atattgtaca
    33241 agaggtcaaa gatcagttag cgaaattggt agaaaacact gacaataagc atatcatgga
    33301 tttattaaaa acattcacta agaaaacggc tgctacgttg gacgatttga aacaactata
    33361 caatgtggca ttagacccag cattaaataa aatggtaatt ctaaaccaaa gcggatatta
    33421 ccacctagat acattgaaag attcagatgg acgttacatt ttacaacccg atgtgacagc
    33481 acctagtggt aaatcattat tcggtatgcc agtagtattg attgcagata cattgtttgc
    33541 caatcctaaa gctggaacat tccctatgat tatgggggat attgcacaat ctatctttgt
    33601 tgctcgtaga aatcaagtaa cgactcaatg ggaaaaattc gattactact cacaaggact
    33661 tgcagtgatc gttcgcaacg attataagaa aattgatcaa aatgcttcag tgtatattga
    33721 gtttacgcca gttgtaacac caaaagtata gaaaaatatt gggcggcggt ttatcccact
    33781 gccttttttt cattaggaga gataaaatgg taattttgga tagcataaaa aaaagtatgc
    33841 ggatcgatca cactattgat gatgacttta ttcaacaatt gattgataca gcaggcgaat
    33901 atataaaaag tgctattgat agtagcgcaa cggataaaga tatggataat tatcagcaat
    33961 ttgatttggc ggtgtcatta cttactcaac attggtattt gaaccgtcaa gaagccagca
    34021 gcgaacggat accagtaacg gtacaagcat tagtacaaca aatgagaggt gcttattatg
    34081 ccaatcatta agaatgtaaa tgaattgact gagagagtca agtttaaaaa gacaaaacgg
    34141 gtaaaagatg aagatgggca gatggtggat agtgaagaaa ctgtatttga atgttgggct
    34201 aatgtgcgtt cacaaatgct aaaagacgtg cttgctagtg taggtaccat tcttgaagga
    34261 acgttgacgt ttattattcg atacgatcaa gattatgaac taacaaacga tatgaaagta
    34321 gcttggaaaa acaagagtta taaaattatt tcaatcaatg aaggaacggc gtttaaagat
    34381 tatacaacga tcatagctaa acagatttct tagaatgatt acacttgtaa acgttgtggg
    34441 tatttagtat aattaaggta gtaaatgaag ggttagctgc ctgaattttg caagacttag
    34501 ctagtcgaaa tctattgttg gactgaaaat tgtaggtgtg gttgcaaaca tgctggacta
    34561 agaaagatga tagatgatga acgttcaatt ttaatacagg catgctagga ctgattggat
    34621 ggttttggca agctgattgt gtagaaatca aagaacacgt ctgttgtgga cgtgtttttt
    34681 ttgttataat tcaaaagagg tgatttaatg gacaaagcag agtttacaaa tttttattgg
    34741 aagtattatc tacatttaga aaatgagttt attaatacca cagattttgt gatgctcgat
    34801 gagaataatt tcaaaacatt ctctattgaa tatcaaaaac tactactagc gattggttcg
    34861 gagtgtgaga ttattttcaa agaactttgc ggatttgata gcagtagcaa taaaagcata
    34921 actgatttta aagctgttat taaatctagc gaattaatgt gtttggataa tggtgttagg
    34981 gttatgaatt ctttaacatt gacttcacta aaaccttttg gagatgggtg gcctgaagat
    35041 acaccagatt ggtggaaaat atataacaga gtaaaacatg gtcgttcttc aaattataag
    35101 gaagcaaatt tggaaaatgt gttgtatgct ttagcaagtc tatatttatt agaggaatat
    35161 ttgcataaga aagtaatact agaaggtgaa gtagacttta taagtccaga gtcaaatcta
    35221 tttacgttat cttgggaaag aaaacattcg tcgatgcaaa aagtgacgtt tgaaaaagtg
    35281 gattcaaatt atgtagctcc actcattgat tttaaagaac aagaagaaat ttaaaaaccc
    35341 ccacggctta ttgaagtttt tgtatgccat attgtatgcc atttggttta aatcgcttga
    35401 aaaatagtga tagataactc taattcattt agtcataaac gctgttttga taacctttat
    35461 agttctttga tatgggtttc tcattcatgg cggaagaaga agaatagaac aaaagaatga
    35521 attaataaga tataaaaaaa gggattgtga caaacgtctt gtcacaatcc ctttttccaa
    35581 ataaacgatg ttcaatcagc tgcccctcgg cattcgctcg aaaaacggcg aaatatgaag
    35641 aactattttg ccatttgttt ctctaataat caggtcaaaa cgttgacttc acagtctctt
    35701 ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatattc aacaaacatg
    35761 agaagctgtt ttttgaaatt gttccttgtg actcggagct gaacggcagg ctcacgacct
    35821 ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagttcaacc aaacgaaaat
    35881 tatgaggagc tatttttgtt tggctgttct tattacttga tgcaggacag ctttttcaca
    35941 acctcttttc gtttagcact aaatttcctt aaaaagtaga aaagctttcg aagcctcgtt
    36001 tgtttctttt actgtataat aaaagaaaag agtaacaatc gctaaaagat ataaacaaga
    36061 gaaaggaaat acaagatgat tggtctgatt tattttgtgg tgatcgttct ggcgaacaca
    36121 gttggcgcta tttccgggat gggtggaggc gtgttgatca aaccgattct tgatcttatc
    36181 ggcgcccatt ctgttgcagg gatttctttt tactcaacag ttgcggtatt cacgatgtcc
    36241 attgtttcga ctgtgcgaca agtatcaagc gggaaatcac tgaattggca gattgtcggt
    36301 tgggtatcta gtggagctgt tgttggagga gtagtcggca acattgtatt cgaagttttt
    36361 ttacagttat ttgaaaacga gaagcatgtg cagctgatcc aaattttttt gacggtattg
    36421 accttggttt ttgcgttctt ctacaccaag catcatcagc caaaatttca tttgacgtca
    36481 tggacttggt accttatatg tggggggggt tctgggcttt ttagctagtt ttttaggtat
    36541 tggtggtgga ccagtcaatg tttctttgtt gatgctgatg tttgcattgc caataaaaga
    36601 agcaacacta tattctctat ctacgatttt cttttctcaa ctggcaaaat tggtaacgat
    36661 tgctttaaca agtagcttta tgcgctttga tttatctatg ttgttttatg ttatccccgc
    36721 tgctgttatt ggaggtttgt ggggagcaag atttagcagg gttttgtctc caaaaaaagt
    36781 gacctttatt tttcaagtaa ttgtcctcgt ggttcttttg gtcaattgtt acaatgcctt
    36841 tgtcatctta caaacattag gagaatctag actaacgcaa gtcaaaatca ctgaaacata
    36901 ttgttctaaa tgaaaaaaag agtatactat gataaaaaaa ttcattttaa aaaatcagaa
    36961 attggctggt gatttggatg ttacgggaaa tgcgtttaga agatgcagaa gcactacgtg
    37021 aaatcaatgc ggatcaatta gggtatgata taccattaac cgttacggtg agacaaatga
    37081 agaaactgct gaaggagcca gagaaaaatt ttttcttggt ttatgaagat cctacttcta
    37141 aaaaagcagc tggctatgta caagcggagt cctatgaatc gatttttagc gatccaatgt
    37201 tcaatattat ggcacttgct gtgtctaaat atgctgaaaa aagaggcatc ggaaaggctt
    37261 tgatgactgg cgtagaagaa gaagcgaaaa aacgaggaat aacgacaata aggcttaatt
    37321 ctgcagaata cagagtagaa gcacaccaat tttatgaaca catcggttat cattccgata
    37381 aaatgcaaaa aagatttttg aaaatcctgt aaatctttat caaaaaaata cccgatcacc
    37441 aagccgatac ttgatgatca ggtatttttg tttttttgac taagaaagta attgcatcaa
    37501 gaaaccagag atgatcaaaa cgatggcacc accaagtcga ttgcccattt gagcaaaagc
    37561 aataagttcc atccgattag ctgcagataa gacggctaca tttcctgttc cgcccatcga
    37621 gttgttgcat aagccagcag taatagttga ttcaattgga tataaaccaa acaaacgacc
    37681 gacaaatcct gaaacaactg aaatcgcgat aacacttgtc agacataaga caacaaattg
    37741 ccatgtcaaa gcagaagcta atacattcag attcagcaac gcaataccga taccggcaag
    37801 aacagctgct gtcaagtttt tgacaatcaa gttattgaac atgatagctg catcttcata
    37861 atactctggt aataggttgg cgattttaca gatgacaacg ataataatca taaatgcgta
    37921 agcatggact ttaggaacaa aatagttacc aatttgtcct aagatgaaga aagtcaatga
    37981 taccatcaaa ccaacaccta actgtggaat ggaaggtttc acagttcgag gttttccatc
    38041 gccaagatcg cctttttcca ttaatttacc attgccgttg aattttggca ttgattgtcc
    38101 agcacgagca actaatgctg caccgatgat agctaagaca tttgtcattg ctgtcgcagg
    38161 aatcaggcgg gaaatgatag aagaagcatc cgtacctaaa acatttgcat aaatcgttga
    38221 taaaggaacg gctccggctc caacaccacc agccatcgca ggtaatgaga tgtataaaac
    38281 ggaatttgca aaaccatttc cgataagcat gcccacgagt cctacggcaa aaaaggagac
    38341 gaccattgaa ataagtgcaa cgggaataaa acgaacagaa gctttcatca atagattacg
    38401 gttcatacca aggatgctac cagtaatcaa ggcagcaata taaaagtccc aaaagcccat
    38461 attgctgaca aagttttctg tggatttcac tacatattca ggtaagatcc caaatgtagc
    38521 aatcgcagca gaagcgaaga tcgcaaaaac ggaaccacca cccaaataac ttttgacgat
    38581 agggattttt gtcccgataa aatgaaataa attaccgaaa ataaccaaaa ctgcgattgg
    38641 tccaatcata ttcgtcggta atttccctaa agccattgca atgatcagga caaggaccat
    38701 gatcaaataa agtggcaaac ttacgccggt gatttttgtg gaccaaaaac cttctttttt
    38761 cttttcctca actgcagttg cagttgttgt tttttgtgcc atttttacag atcctttctt
    38821 attctgtatc atatgaaaaa agccaaagaa aagcgacaaa agagcaggag ttggctggcc
    38881 gctgttttgc cgcagtcgct tttttctttt tagacgtcat acaaagtttc tgtatgttct
    38941 tttactataa cgatcttaat cttctaaaga tttatattca ggaacccatt tagcagattc
    39001 aactgcttct ttgatatccg tgatttcttc acgattcaag ttttgtgcga caactgattt
    39061 tgcgacagtt tcagcaatcg tttgagaaaa ttcagtgatt tttgctactg gaggcaagat
    39121 agctgctcct ggttttgtta catcaacgat tccgcctaat gctcgactag cttgagaaat
    39181 gatttcagca tttactcgtg tagctgttga agcaatcaaa ccaagtccta aacctgggta
    39241 catcaaagca ttatttgctt gaccgatttg gtaagttaca ccattatatt caacatcagc
    39301 agcagggata cctgttccaa ctaatgcttt tccgtccgtc cattctatta aatcttttgc
    39361 ttttgcttct gccaattttg ttggattaga caaaggcatg atgatcggac gtggtgtatg
    39421 agcagccatt tctttcacga tttcttcagt gaatgctcca ggttgggtag aagtaccaat
    39481 catgattgtc ggatggatcg cttttacaac tgcttctaag tttgtcaatt catcagcatt
    39541 agtgaaatct gagcgtttgc gagcgaatgg gatttgacca ggagtcaatc cttctgtatc
    39601 ttcaaacaat aaaccttgtt tatctacttg atagaaatgt tggcgtgctt cttcttcagg
    39661 tacaccttga cggatcatct catctaaaag gatgtttgcg atacccacac cagcagtacc
    39721 agcaccaaat gtcaaaatta tttgatcttt caattgttca ccggatatat tcaatccgcc
    39781 taatacacct gctaggacaa caattcctgt accttggata tcgtcgttga aagtagtgat
    39841 ttgatcttcg tatttttcta agatcgctgc agcatttcct cgtccaaaat cttcccaatg
    39901 caataacaat tctgggaata attcggtagc tgattgaacg aattgatcga tgaattcata
    39961 gtaagtttcg ccttctacac gagcatgacg attgcctaag tataagggat tattcaataa
    40021 ttcttcatta ttagtaccag cgtcgatcga tacaggcaaa acttgtgctg gattaatacc
    40081 agcagcagca gtatagacca tcaatttgcc gatagcgatg tcaacaccgt tcacacccca
    40141 gtcgcccata cctaagattc cttctgcatc agtcacaaca atcaaacgaa tgtctcgtcc
    40201 atctgccgcg ttcttcaaac ttgctttgat gttttctggt tcgtcgattg aaagaaatgc
    40261 agcatcttgc ggtttcaaga aaatttcgtt gtattgttcg attgaatccg caacgacagg
    40321 atcataaacg acaggcatga attcaactaa atgttcgccc atcaatttat aaaacaatgt
    40381 ccgattcgta ttgaatagat tcattaagaa gatacgtttt tccaaatctg tttgtttgct
    40441 taagtattga ccataagctt gaacagattg ttgttccagt gtttggacag tacttggaag
    40501 cataccagtg attccatatt ttgcccgttc ttcttttgta aaagctgttc ctttatttag
    40561 aaaaggatta tttaataagt ttaaccctgt aagcatgaat gattcctcct aagttttaat
    40621 acaaacgaag tatagaagtc agatgatgat atagtcaaaa agagcaggta tgataaattt
    40681 tatttatatg taatatagcc aaaagaaatc aaaatagcag tatttgttct ttttatatcg
    40741 acaatccttg cttgaatact atgcgtatct cttatatgct agagaaagaa aagtagaaga
    40801 ataagggata gatggaatgg ataaatagga tttctgagga aaaaagcaca tgatagcgtt
    40861 gataggagtc acctatgtca ggtgataaaa atacataagt agaaaattaa gaggaggaag
    40921 agaaaatgaa agaagttcaa ttgattagaa aaagcgaact atcagaaggc ggttgcaatg
    40981 catgtggcgt agtagaagcg acaagctata cattaaaatt agattccaat caagcaataa
    41041 tctctgaact gacagtaggc ggtcttgtgg acagtctggc gcttgctgaa ggttttactg
    41101 gagaagatat ttatgagatg ttttcagaag tccgtcagtt aaaaaaagga gaaaaatgca
    41161 tagaggttca tcatgagagc ccgaatgtga ggttcaaacg tggtgataat gagatgatct
    41221 tcaagaatca tgtgtctgat cacactgaac tatatgaaat cgtgaatcaa atactgtctg
    41281 ggatttttga attaggacct tatgagttca aggaagaaaa cggtaatcca aaattaaatg
    41341 aagaatggca agaaacgatt gaaacacaaa gaaacaatcc acacttattt caatagtctt
    41401 ccacagtatt tcgtaaaaat gttgatgaaa aagaaacacc gtgaatcaga ggtcgtgaaa
    41461 aacctgccct gcatcaagta ataagaacag ccaaacaaaa atagctcctc ctatttttcg
    41521 tttggttggg cttattaccg cagatgcaag tttttgaaca ccgtttaagg aagagactgt
    41581 gaagtcaacg ttctgacctg agtattagag agacaaatgg caaaatagtt cctcatattt
    41641 tgccattttt tgagctaata ccgagggtca gctgattgaa caccgtttta tcgagaggtt
    41701 gtgaaaaagt cgcttagttt cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat
    41761 ttttgcataa atttgactta ataccgagaa aatggctttt gaacaccgtt tattcaaaaa
    41821 aagggacttt gacactactt ttgtcaaagt cccatcggtt ttttatctat ttttattaca
    41881 atcatttctt ccagagtagt gacatctgga atggctgaaa tgtaaacaga ttatttgcct
    41941 gtatcaatgt cgaagagtaa tataaaaagt gtaaacgcta tcatattgtt catcattcaa
    42001 attttataga gagaaggcat tgagatggaa aaaacatatc aagcaggtac aaatgaagga
    42061 atagtagatt ttatcaatat ggaagattta gaaatcgcgg cttcacaagt gattccagct
    42121 ggtggttatg gttacatcag cagtggtgct ggcgacttat ttacttatca agaaaatgaa
    42181 cgggcattca atcatcgttt gattattcct catgtattaa gagatgtaga attacccgat
    42241 acaactacac attttgacga agaaatgctg actgcaccaa tcatcatggc accagttgct
    42301 gctcatggat tagctcatgt gaaggcggag aaagcttctg caaaaggtgt agcagatttt
    42361 gggacgatct atacagcaag ttcttacgct tcttgtactt tagaagagat aagagaagca
    42421 ggcggggaga aagcaccgca atggtttcaa ttttatatga gcaaagataa tggaatcaat
    42481 ttggatattc ttgaagtggc caagcgtaat ggagcaaaag caatcgtatt gacagcggat
    42541 gcaacagttg gcggaaaccg tgaaacagac cgcagaaacg ggtttacctt cccgctgcca
    42601 atgccgatcg ttcaggctta tcaatcagga gtaggccaaa cgatggatgc tgtatataaa
    42661 tcttccaaac aaaaacttag tccaaaagat gtagaattta tcgctgctca ttctgatttg
    42721 ccagtctacg tgaaaggtgt tcaatcagaa gaagatgtct atcgatcatt agaatctggt
    42781 gctggtggaa tttgggtatc taaccatggt ggccgtcaat tagatggggg gcctgctgcg
    42841 tttgattcgt tgcaatatgt ggcagaagca gtagataaac gtgtaccaat cgtgtttgac
    42901 agcggtgtac gacgtggaca acatgttttc aaagcaatcg catcaggtgc cgatcttgtt
    42961 gctattggca gacccgttat ttatggattg tctttaggag gaagtacagg agtccatcaa
    43021 gtgtttgatt tctttaaaac agaattggaa atggtcatgc aattagctgg aacacaaaca
    43081 gtagaagata tcaaaaaaat aaaattgcgt gagaatcgtt ttatttaaga aataaatgac
    43141 gaaaaaaata ggagtgcggc agatgatgcc gtactcctat ttttatagtt caaggatgtc
    43201 actaatagaa taatttgaaa cgaaaaaata aaattgaaat attttttcat acttaataaa
    43261 aaaggaagaa tattcatgta aacgatttta ttgttttttt ctcttttata caaaaagggt
    43321 cataatttca agtaagcgga tgaattcatg aggatttttt gcatactgtc tctactatac
    43381 ttaggtcact aagaaaaagg agtgatttat tatgaaaaag aacatgattg ggacattatt
    43441 actttcaaca cttcttctag gcggcttggc aacaccagct tttgctgagg gtcaagcgac
    43501 ttcaaaagga gatattacct ttactgaacc aacaaatacc gttgagcctc ttaaccctac
    43561 tgatccttca aaaccagtag agccagcaga tccggaaaat ccagcaactg gccagacagg
    43621 ttctttaaca ttggatgtag ttcctgaatt gccatttggt acacacgaga ttgaaagcgg
    43681 aacaaaaacg tatcaagtag acgcttcaaa aaacgataca ccttatctcc aagtatcaga
    43741 tcgtcgaggt gtaggtgcag atggccaagc acaagggtgg aacgtgacag tttcggtttc
    43801 agattttgta aacggttcac aagttttgca aggcgctgaa ttagattttg gaacatccac
    43861 tgttaaaagt acgtcagata acgaatccac tggaccaact agtcaaactg taactgggtt
    43921 gagtaaagca agtgcagcga caccaatatt tacagcagca aaagatcaag gtctgggaac
    43981 atggctttct gtttatgatc cagcaaatat cacactgaaa gtgccgaaag cagcagctgg
    44041 aacattcacg gctgatttga cttggaacct cgtggctggc ccagtagcat agagtatgaa
    44101 ataaattcaa caaactgcgg tgaaaatact ataggtgaag gattttttac cgcagttgtt
    44161 gttgaaagga tgtggtggac aatgaatcaa agtaaacgac tgtttgtttc gtttttttct
    44221 attttaagtc tatttttcat ttttccgtca atcagccaag caaaggattc agcaggagac
    44281 tttggaatca aaccggtatt tccagagaac caaatagata aagcaatcgg ttattttgat
    44341 ttattagtgg caccggaaca aaatcagaca ctagaagtaa ttatcagcaa ttcttcagat
    44401 gaagaacgaa catttgaggt ctctgtcaat ccggcggtaa cgagtgatgg tggaacgatc
    44461 gattatagtc aaaaaaaccc aacgttagat gagacattgc cgtttgatgt ccgtgacgtt
    44521 ttgctcattg cgaaaaagga aatcaatgta tcagcacatg cagaaacaac tatacctata
    44581 gaagtgaaaa tcccagcaaa aagctttaag ggacgtgtat tagcgggtat tcatgtttca
    44641 cctaaagaag aagctgaaac agaaaatgct acagaaggag cacaaatcaa aaatcgtatt
    44701 gcttataatc tcgcagtcgt tttacaagaa agtcaagaga cgatagaacc ggatctgaaa
    44761 cttttatcag gagatttgga cgaggtcaac gcaaagccaa ctgttcagct tcgtttccaa
    44821 aatcctcagc ctaggattat cagcaatttg atatttacga gcaagatctt ttatgaaaat
    44881 cagctttata ttgaaaatac gtctaatgaa tttttagttg caccaaattc gaattttcac
    44941 ttgaatcttg atttagctgg tgataaagca aaagccggcg attaccgcgc agaaatcata
    45001 gcaaaatcag gcgacagtaa tgaatggcga tttacacaaa actttacgat caaaaaagaa
    45061 aaagcacaaa aagtaaatga aaattccgtt tttgcagtac aagaacaatc tttcccatgg
    45121 ctttatgcag gattggttgc tgcagttcta gtgttatggt tgattttgct gtttttgtgg
    45181 gtccacaaga aaaagaaaaa aggagaaaca acatgatgag gtgatttcgt tgaaaacatc
    45241 gatcttattg ggagggatta ttgcaagttt gctgttcttc agctggcaag agccagtcca
    45301 agcagatacc aaaagttcag gtacgattac attttatgat ctaagtccag ttccgcatcc
    45361 aagtggttca gtcaatggcg catcaaaaca gcagacacag ctaccgattt ataaagaatc
    45421 taatcaacat ggcgtattcc cggagacgaa tgaagaaaag atccgttttc tttatttgat
    45481 cattggtata gaattatggc ttcttgcgtg gttgttgtta tggagaagaa aggaaaagga
    45541 ggatgaacat gttgctacaa aataaatttt cattttttcg ctccttcttt tttacttcgc
    45601 tttttgtttt aagtatattt atcttttcac cttcggcaga agctgttgga gcaacgcctc
    45661 ctgccatttc ttatgatcca tacacgtatg aaaatatcgt acctccaaat ggtggacgta
    45721 gtgcagtcaa tggcgggaag cttgtccaac taactggggc taaccaattg cctaaattga
    45781 ctgctactta tttgaacgat ggttcaaatg atccttggaa aggtggcacg gtccgttttc
    45841 attgggaaag ccatgtgaaa ctaacaaatg tacttgattt ggtgaatatc actgcacaag
    45901 gatttgcatt tgtggcgatc atcaatttgc cggaacatgt atcaagcagt gatgtattaa
    45961 aagcaattga ctggaacaac gcctacttgt atattgccgg tgcccccatc aaggttacta
    46021 gcggtagttt attatcagca ggaagccata ccttgcgttt tgggatgggg tcgtgggata
    46081 caaatactct attggcagat attattgcta ttattacgcg aggcgaattc aacgtggacg
    46141 atatcccgct tcaatttgat gttacagcta atgtcgcgaa gatgacagaa aacggagata
    46201 aatatgatac gagtccaaac aaaattttga ccaagggtaa attacaacct tctatgtcta
    46261 aaaaggccgt tttttctgtt gatttttatg attcgaataa tattatccaa gatcgtatgt
    46321 tcaattcgat tttagctcca gctgcaggta gattttatcc taaattaact ggcggaaact
    46381 tgaatccagg agcatatgca ttttctgcaa gtgcagagat aaatacgtgg aatagttaca
    46441 tctcaacaag tgattcaaca ggaaaatata atacatttgc tgataccaca gaatacgtgg
    46501 atggaaataa ccagaacctt ccaggctcta ccaatagtcg gacattgacg atggggctga
    46561 ctgaaggacg ttttgatcag ttgccattta gtcgctttga tcgcgtagtc aactatttta
    46621 caggaaaaaa cgttacacaa ggagcaatac tgactcatac tccagcaagc acaagtggat
    46681 tgaatcagac agccaatatc gtgtacagtg ggaaagatgc gcaaggcgga ttgctttctc
    46741 ctgttgcttt aaaggtattg gagcagtaca agctagacgg aacggtttta ccaacaaact
    46801 taacagccga tagtcagtgg ggaaagctcc tttcttctgc atcgtaccaa gtcacggcca
    46861 attggtcagc aactgaacta aaaaagggtt cgatccatta caggctttat tcgaaaggaa
    46921 cccaaaaatt agcgacagga tggacagatc aacttttcca aacgatccaa tcaaataacg
    46981 ggggtgtaaa tacagctact tctacactac ctgcattggc ttcagggcaa tattattttg
    47041 attatcgctt ggtggacgat gtacttacta acatgtatcc gtcttttgcg tacaaatggc
    47101 agtctgaaca agctggaatc accactcttc ctcaaataac agtggctaat tttccatctg
    47161 tgagtgcaga tagtcagcta aaaaacctta gcagacaacc tgagacagga gatcccctgt
    47221 ctgctctatc aggagatacg attcaagaaa atgtgacatt cacgttaaca aaatctgggg
    47281 atgcgataac cgacaaaaaa atcacgatct cacttcctga aaatactact tatatcaatg
    47341 gttccttaaa attaaatggt acaactatcg cagataccgg cattcaacaa ggagtttctg
    47401 tcccagcaga tctattgagc aaaataggag ataccattca tcttacttac aactatcaat
    47461 taaatactgt agatacttct gtacaatccg tcagcatact gacaaaggca gctgtacttt
    47521 ccagcaatat tacattagca gatggtgcta agcttcctaa cccagttgtc caaacatctg
    47581 ctaaaacaat tcttgtcccc aaacaagaat taacattagt taatgtacca gatgatttta
    47641 catttggtaa cgatctcccc aaaccgttga agacaagtta ttatgaagca aaaggcgatt
    47701 tttcttttga tgtgcgggat actcggcttc caagcacgtc tccttggcaa ctgacaggga
    47761 cattgacttc tttatttaag aatgatcaag gtcaagaatt aagcggaaca aaattatact
    47821 tcaatcattc tggatcaaaa caattgattc agcaaggtca aaacacgttg atttatgaga
    47881 gtgatggaac agctaaagga gaggttctcg ttgactttcc cgataccgat ggtttactct
    47941 tagaggtaaa tagcagtacg aatgctcaac ctggcgcaac gtatcaaggc atggtcacat
    48001 gggaactgac agctgggcca accagctaag tgattagaat ttgtatggat ctgtgataac
    48061 tgtcacagat cctctttttc aaggatgaga aggaaacttc tgctgttaaa atcagaaatg
    48121 cttggaggag tggacttgga acttttcagt ttgcttgaaa aaaatgaaca acggcaaatc
    48181 aaacttttcc aacttttatt agaggaaaaa ggagagattt ccgttaataa cgtacttacc
    48241 cgcttatcag ttgatcgggc taccttgaaa gaagatctgt tttatttaca agctaacttg
    48301 gaaaatatca aacaattgga aattgttatc caagaaggga atattttatt aatcagacat
    48361 ggaaatatag cagcagtcga tgtttattat cattgctact tgcgctattc tacaaaatat
    48421 cagatacttc tttatctgtt ggaaaatggc tcatttgatc ggcagaaact tgtcatggaa
    48481 cttaatatca gttcggctac tttgtctcgc agagtgaagg aattaaatga ttctttaaat
    48541 gagtttcatc tgaaaataaa aagcgggcgg ctgaccggtc cagaaagtca aatttgttat
    48601 ttttatttcc aattattttg gtttggacgt ccttatcttg aaaacaaaaa agaatacagt
    48661 gatttatcag gaaacacttt tttagatctg ctgcaaaaag agtttagttt tactattaca
    48721 gaagaaggac gattgaaatt tgaaatatgg ctttccatta tgaagaagcg aatgcgctgt
    48781 acgaaaaaat ccaaaccaga ttatagcaat cttcctgttg gattgtttca aacgacacaa
    48841 tttttattgg acctccatac atttttgacc cgttacttct ttcaatatgc ctttccttgg
    48901 aatcaaaatg aaacgttggt tttttatctt ttcatgatca gtaactttac attagataca
    48961 cataactcgc atgtccagga gtttttgata agcgaaaaat acaaagacga cgtagtagct
    49021 caattgaatc gaacgtttaa acaatcgata gaagaatatt tctcttttta tgtttttcca
    49081 gaagagattt cgcaaaagat tgcgttgaca gtgatgcaaa atcacgctca ttttgcctat
    49141 tttagaggat ggatgaatat ttttggccaa gaaggattaa aacagcggtt aactgaagta
    49201 gaagataaac aattcctggt tttatgtgaa agattggtca gacaaacatt tgctattgtt
    49261 gattttactg aagtagacga ggaaaatgcc cgctttgaat tatttgctcg ctatgcatct
    49321 attttgcgga cgctgatcaa gcagttagaa ttacgtctgc aaattgctta tgatcttcct
    49381 tatgaaaggc taatggcgga tatcgtgaaa gaaatcatcc aggaacaatt gctgaatacg
    49441 atcaaatatg atttagtcga atacgagaaa gaaaagcagt atgatatcat tttgactagc
    49501 caattgaaag agtacccgtc acagaagtcc gctaaagttt ttgtattcac tagcaacgaa
    49561 tataattatg atttccccta tttgaaccaa tttttaaaag aatgttattt agaaaaattg
    49621 aatttaagga tgaataaaac atgaaaatcg tgatcattgg tgcttcccat gcaggcatca
    49681 cagctgccct aaatctaaga aaacttcaac cgaaagcaga agttttactg atcgatgaag
    49741 atcataaaga tggactaggc tatgtttcaa atggaatcaa cctttattta aaaggaaaga
    49801 tacgtagttt agctgaagtt gcccataaca tgcggactct tcaagaaagt ggggcaaaac
    49861 tgatcaccga atggagagta actgaacttg atccagacaa acatcagctc atcctcgcat
    49921 ccaaagaagg aaaaaacgag accataacct atgataaatt aatcgtagca acaggttcct
    49981 ctcctgtcac cttgtataaa caaattgaag ctgaaaatgt ctacacctat aaaaatctag
    50041 tgcaatctaa gcaagtactt gccgctttaa aagaagcaaa agaagtagtg atcttcggtg
    50101 ctggttatat tggattggaa ttagcagatg ctttgagaaa taaaggatac attattcatc
    50161 tagttgatta tatgccgaat gttttatcaa gatatttcga taaggatatg atcaacagtt
    50221 ttcaaaatca gctgcaaact aagcaaatca atttttatcc aaatgaattc ttgatcgatt
    50281 ggaaaaaatc agaagagaaa gtagtatccg tacaacttct atctcaagca ataaaggcag
    50341 atatggtcat tttttctgcc caaactcgtc ctaatacgac attgttaaaa gaaaaagtgg
    50401 ctttgtatga ggatgagact gtgatagtca atgaatatct gcagacaagt gatccggaca
    50461 tctatgcgat tggagatatc gtcccagtca gttttgacaa aaataaacgg catctctttt
    50521 tacctttagt tactcgagct gtacatatgg caagagcagt agcgctaact ttgagtggtc
    50581 agccaaccgc ttacgacctc agacaaaaga taacagcagc agtcattata gactactttt
    50641 taggaactgt agggctgaca gaagatgaag cgccattctt ggaacaaaat accggttcgt
    50701 gttcaggaga atttgatcta tttccacaat acgatgaaga aaacaaaaca gtaaatgcaa
    50761 agctgatcta ccatccggat acattagaaa taattggcgg acagctgata agccaagaat
    50821 tcttattatc agatttgaac ctgcttgcgg atatcgtgaa acacaaaact acgattccac
    50881 agctagcagt tgaggatttt ggatttctgt ctgaacacac ctctcgtttt cactacctca
    50941 atgagttagc ttttaaagta ctagctaaga gagcaaatag caatagagta gataaaagac
    51001 tgtgagaaat atcacggtct tttattgtgt ggggatataa taaatattaa tcaatcgaga
    51061 ataaaaaatt catataatga aatagcggaa gaattttttg agcataatat tcgccaaaaa
    51121 tttatcaagt gataaaattg ttgtataaaa tgattatttc aggaggagga gttaaatgtt
    51181 tttagcatta aatgaaatta tgcattcaaa gttacgttat gcgttagttg caggggtgat
    51241 gtttttgatc gcctacctgg tatttttttt gactgggtta gcttatggct tagctcaaga
    51301 taatcgaaca gcagtcgaca agtgggaggc agattcgatt gtgctttcga aagatgcaaa
    51361 ttcaaatcta gggatgtcca tgattactaa gaagatagca gaagaagtcg aaggtgggaa
    51421 agtggcgtat cttgcccaga cacctggagt cgtgactagt aaagacagta cagaagaagg
    51481 aaaaatcaat gttagcttct tcggtatcga taaaaatcaa ttcatcatgc ccaatcttgt
    51541 tgaaggaaaa gcttttgata acgatgatga ggcagtaggc gatattagtt tgaaagaaga
    51601 atatggtcta gccgtaggag atacagttaa gttatctggt agtgacaaga cgtttaaact
    51661 aactggcttt acggatcatg ccaaattcaa tgtctcacca gttctctata caaccatcaa
    51721 tgcttaccaa gagatccgct tcgaaaaaga agatacaagt gaaaatgcac ggattaacgc
    51781 aatagttgtc agaggtaaaa tcagtaattt acctgaagat ttagaacaga tcaaaatttc
    51841 taaattcatc aatgaattac ctggatataa tgcgcaagta ttgacttttg gatttatgat
    51901 cggatttttg atcgttattg cggctattgt gatcgggatt ttcatttatg tactgacgat
    51961 gcaaaagatt aatatctttg gtgtaatgaa agcacaaggg atcacaggag gatttattgc
    52021 gcgttctgta gtggcacaaa cattcatttt atcatttgtt ggtattcttc ttggtctatt
    52081 aggaacagtg ggaacatccc ttgttctacc tgatgcggtt ccattccaaa gcaattggct
    52141 gttttttggc gtcattagct tgttgatgct ggtagtagct gtgttaggcg cactattttc
    52201 tgttcggaca atcgtaaaaa tcgatccgtt gaaggcaatt ggataagaag gaggaagagc
    52261 atgaaagcaa tagaatttaa agaagtagaa aaaagtttcc aagatggcga ccaagtagtt
    52321 aaagcattga aagaaacaac catgagtatc gataaagggg aatttgttgc aatcatcggt
    52381 ccttctggtt caggtaaaag tacttttttg actttggcag gcggattgca gacgcctagc
    52441 agtggctctg tatttatcaa tgatcaagaa ttcagtaatc aaaaagaaag tgaacgcgtt
    52501 aaactacgtt ttaaagaaat tggctttatt ttgcaagctt ccaatctggt tccttttctg
    52561 acagtaaaaa agcagttatt attagtagat aagatcaaac atgaacatcg tcaaaaagaa
    52621 gcaaaagaat tatttgagca gcttggcgtg gataagttga tcaacaagta tccagaagaa
    52681 ctatctggtg gagaacggca aagggtcgca atcgctcgtg cattgtacaa tgatccgtcc
    52741 attattctag cagatgaacc aactgctagt ttggattcag aaaaggcacg agaagtagtc
    52801 gatatcttgg caaaagaatc aaaagacaaa aataaagcaa cgatcatggt tactcacgat
    52861 acacggctga ttgacaaatg cgatagaata tttatgatgg aagatggtgt attgaaagaa
    52921 aaagaataac caaaaagcag gtggaaaaat ggtccatcga tacaacaaag atacacagca
    52981 aaaaattttg aaagaagcga ctgacttgtt tatgtccaag ggatatctcg gcacttcaac
    53041 aagagagatc gcacaaaaag ttgggatcac ccaacccaat ctctatcatt atttcggtga
    53101 taaagaaaaa ctatatacgg cagttttaga aaaccactta aaagatgttg gaaaagcttt
    53161 gagagaaatc gttcaaacaa gcgaaacggg atttcaagta accttgacaa aaatggcgca
    53221 gtttttgatt gagacacatc tggtcgattt gtttctgatg cttcacgatc ttgaaagcaa
    53281 tctttcaaag gaaacaagag atcatctttt ctttctatgg aagaaaaatt atcgtgaacc
    53341 ttttgaagaa atcttttcag caaaccaatc tgtgctaaga aatgggattt caaaagaaat
    53401 cgcagcaagg cattttttct tagtgttagc accttacatc actcggtcga aaagacctgt
    53461 ggaagaaccg ctaaatgtgg cgcaattgat cgatttatat ttgaatggtg tattaggaga
    53521 agcgtaaagc tgttatatag ggagaaaaat agttaggtga agaagcaaga tatgacgaag
    53581 atcgttatat cttgtttttt ttatataggt agtttttcat aaatacaaat ttgttattct
    53641 ctcatgatta tcagggtata tttttattta ttatgtcggt gttccaggaa gtgttttaat
    53701 ttaataacga agaaattaat tgtttgtaga atgaaggaaa ctatttattg ggtagatgaa
    53761 tcaccacgaa tagtcaaaag gagatttcag gatatgccaa aaatgaaaaa aatcatttta
    53821 gcagtaatgt tattttctac ggttatatct tttacagccc cttttttgcg tacttaagct
    53881 gattttaagt tttgaccctt agtctggatc tacgaggtga gagtcaagaa tgaaaaaaat
    53941 aattggagct tttgtatttg caatcattat cattattatc tgttttcaaa tagatgtttt
    54001 tggtcaaaaa aagatagttg gacaaacggt cagcagtacg aacagtacgg ttacaaagac
    54061 aaaggaatca tttgatgata cagaacaaga gacattaatc aaaatcggaa aaaatgaccg
    54121 tacactatat acagatgact cgctaactac cccacttgca actataaacg gcggagagct
    54181 ggcggagttt ctttctgaaa caaaaaatgc ttatcagatc aagacaaatg atggctatac
    54241 tggatattta gccctcgaag atggaaccaa agtcacaaag aatatacaaa caaaaccgga
    54301 agagctatct gatgcagtga ttgtattaga tcctgggcat ggcggaaatg atactggggc
    54361 attaagcaat gatgaacaaa cagaagaaaa agatataaca ttaagtacag ccataaaagt
    54421 aaaaaaggcg ctagaggatg ctggagcaac ggtttatttg acccatacta cagatgaatt
    54481 agtacagctt ggagatatat gcgactacag tgaagaaaaa aaggcagatg tctttatcag
    54541 tttacacgca gactctacgg aatatgcgaa tgaagcaact ggaatcacga catattatta
    54601 ctatggacaa gaggaaacat tagcacagac aattgctgac tccttcactg atctgcctct
    54661 ggattcaaga ggaatctcca ctgggaacta tcaagttctt cgagaaaatc ttcaaccttc
    54721 tattttaatc gagatgggtt atatgaataa tgatagtgat ttaaaggaat tagtaacaga
    54781 cagttatcag cagcagattg ctgcaagttt gacacaagca ttgaccactt attttcagta
    54841 ataaataagc ccagccaaac aaaaatagct cctcatattt ttcgtttggc cgggcttatt
    54901 atcgcagatg caagcttttg aacaccgttt aaggaagagg ttgtgagcct gccgtttaac
    54961 tccgcgtcac aaggagcaat ttcaaaaaac agcttctcat gtttgatgac tattgtgact
    55021 tgtgaccgag gagttggcat gagaacaccg tttattcgga acaagggact gtgacatgac
    55081 ttttgtcaca gttccattat ttttttagca gcttcaaagc tttttctgta ttggcaaaat
    55141 gaagtttggc atatttttca agcataggca tgccgccttt ctccaatagt tttgccgcta
    55201 cttggtcaga tttcgttcca gctcctgatg gaagggtgat tccagcttca gctgatgctt
    55261 tgtgtaactc ttccaaaaag ctggcacgac tgatgataga agcagcagca acagctaaat
    55321 gatattgttc tcctttagta acgaagtaca gtttttcttg taattgattt ttttctaaac
    55381 gcgcatattt tttaaaattt gcttccggcg taaactgatc gatcaaaatc gcttctggtt
    55441 tttctggctc gatttttttt agcagcaaat ggatcgcttg attgtgcaaa gcgactttca
    55501 tccgaacggc attgtatttt ggttggatct cattgtattt ttctggagat aagacaagca
    55561 gtttataggg gatggttttt tttaagatgt cagcaagctg gatgatttgg ctatctttta
    55621 actcttttga atctctaaca ccaagtttac gtaattctgc aagctgatca cgtttcacat
    55681 aagcagcaca tactgttagc ggaccaaaat agctgccatt tccgacctca tcactaccta
    55741 aaacggataa ttgactgata ttttttggca aagaagaggc tgtttttgct ggcttggctt
    55801 gtttatcctg tgcctccccc catttatcag cttctattgc tgcattcgta ccttgaaaca
    55861 tcacttttcc ggatgtataa gcggtaatgg tcgtcccgtc tttttttgca gcaaagctag
    55921 tataaggaat ctgtttattt aacaaataag agcgatagta tgctttcatt tgactaagtt
    55981 ggtcgtttgt tgctttgata atgacgttgc tcataaaatt ctcctcgttt ttatacataa
    56041 aactataccg caactcacga cttttcgttt tctaagagat tgcgagacat agcatttttt
    56101 taaagtttat catataatag ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa
    56161 ccaacttcat ggtacactaa ataaaacttt catcaaatag gaggaaatga ttcgtatggc
    56221 gcacgaaaaa acacgctaca aagcagtgat tgccaaccaa acatatacta ttatcggacg
    56281 ggaaaccaag catcatatgg atatcgtaac gaagctgatc aatgaacagt tagcagaatt
    56341 gaaacaatta tcaccacaaa tggataatga acaagcagca atcttgatgg ctgtcaatgc
    56401 gctttcggat caattgaaga aacaagaaag aatcttggag ctggaagaag aaacagcaga
    56461 attaaagaaa aaaatgatca aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc
    56521 aatcgagaac gaagctcggg aagtattgaa agaaaatggt caagcagatc ataccattaa
    56581 aaatcatgtg gaagcacaac agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa
    56641 atcttctaat taagatcctt ttatcaaaat aagaaaaatt taaaaaagtt agaagagaaa
    56701 ggaacgtagc atgctgagtt tgctgatttt atttttattg ctaattgcgt ttttttctgg
    56761 agcacgtcgc gggtttgctt tgcaaatcat attcgcgatt ggatatgtat tgtcatttat
    56821 tgctgcccag catttttaca aaccattagc aagtcatttg gaattataca ttccataccc
    56881 agctgtcacc ccaacatcaa agttagcatt ttttgatcag gtatttgctt ttcacctaga
    56941 tgaagctttt tatgcaggaa ctgcttttct gattattttg ctaattggct ggctgcttac
    57001 ccgatttgtg ggggtttttg tccatggatt gacgtatgtg ccgatcttga gacaggtaga
    57061 ctggattgca ggaggcatcc tgtcactaat catggcttac gttactatat ttttgatttt
    57121 gcagttactt gcctttgttc cattagtgtg gtccaaaatc aattcggcga gcatagtttc
    57181 gctcgtttta tcgttgagag aacgccattt cttaccaaca agatgtatga gctttgggta
    57241 acgaatattt tgaattaagg aaaatagtgc tggaaaatat ttcgtttttt gtacagagta
    57301 gtttatttaa gaaggctgcg tagcgtatgt ccggcacagc tcttttttca tagtgaaaga
    57361 taaggtgata aaatgaataa ccgaatcctt gaaacattgg aatttgaaaa agtcaaacag
    57421 atggtcagac aatttgtagt gactgcacaa ggaaaagaag aactggcaga attggtacca
    57481 gtgagtgaga aacaaacgat cacaaactgg cttcaagaaa ctgaggatgg attgaaagtg
    57541 caacgtctgc gaggcgggat ccctatacct aaacttgaaa atatccgtcc ccatatgaaa
    57601 cggatcgaga tcggagcaga cttgaatgga gtggagcttg cacaagtttc cagagtattg
    57661 tctacgacaa gcgaattgaa tcgtttcatt gatgatcttt ctgacagtga aatcgaattt
    57721 gcacgtttgt atatgtgggc agatcagtta gtcacgatcc ctgttttgag ccgccgttta
    57781 aaggaagcaa tcgatgaaga cggacgcgtg actgatgacg cttctcctga gttaaagagc
    57841 ataagacaga acattcggcg aagtgaacaa gctgttcgtg aacagttaga tgggatcgtc
    57901 cgtgggaaaa atgcaaagta tttaagtgat gcgatcatca ccatgcgaaa tgaccgctac
    57961 gttatcccag tcaaacaaga ataccgcggt gtatttggcg gtgtagtcca cgatcaaagt
    58021 gcttcaggac agactttatt tattgaacca aaacaagtgg tggatttgaa taatcgtttg
    58081 cgtcaatacc aaatcgctga acgaaatgag attcaacgta ttttaagtga actttctgct
    58141 gaacttgttc cccatcgtca agaaatcatc cacaatgctt atgtgattgg taaaatggat
    58201 ctgatgaatg caaaagcacg atttggtaaa gaagtaaaag ccatcgtgcc gggaatcagt
    58261 gaagacaatc atgtcgtctt gaaacaagca agacatccac tgatcgatca ggaaaaagta
    58321 gtttctaatg acatcacaat cggaaaagac tatcaagcca tcgtcattac aggacctaac
    58381 acaggtggga aaacgatcac gttgaagact ttaggtctgt tacaactgat ggggcaagca
    58441 ggattaccga ttcctgcagg tgaagaaagt caaatcggta tttttgagga agtatttgcg
    58501 gatataggag acgaacagtc gattgagcaa agtctatcga ccttttcttc tcatatgacg
    58561 aacactgtag atattttatc aaaagtcaat gaaaaaagtc tggtattatt tgacgagctc
    58621 ggtgcaggaa cagatccgca agaaggtgct gctctagcaa tcgcaatctt agatgatctt
    58681 ggtaaaaagt ctgcttatgt gatggcaaca acgcattatc ctgaattgaa agtttatgga
    58741 tacaatcggg caaatacgat taatgccagc atggagttcg atgtggatac actaagtcca
    58801 acgtatcgtt tgctgattgg cgttcctgga cgaagcaatg cgtttgaaat ctctagccga
    58861 ttaggtcttg atacagaagt tattgatgaa gcgaagcagt tgatgaatga tgagagccaa
    58921 gatttaaacg aaatgatcac agatttagag aatcgccgga aaatggcaga gaccgaatat
    58981 ctggaaatgc gtcattttgt ttctgaggca caagaacttc acgatgattt gaaagaagct
    59041 tacagctatt tctttgaaga acgtgaaaaa gaaatggaaa aagcaaagaa aaaagcaaac
    59101 gaagtcgtgt cagaggcgga agaaaaagca gaaaaaatca ttgctgatat tcgtaaaatg
    59161 caacagcaga ttgggcaagg aaatgtgaaa gagcatcagt tgatcgatgc aaagacccaa
    59221 ttagcaaatc tacatcaaga agaaactttg aagaaaaata aagtattgaa gaaggcgaaa
    59281 gaacaaaaaa cgttgaaacc gggagacgaa gtgttggtta cgacttacgg ccaaagaggg
    59341 acgcttttac gtaaaaacgg gaatcagtgg caagtagaaa tcggtatatt aaaaatgaat
    59401 gtgtccgaag acgagctgac gcctgttgct cctcaaaaag agccgacaca acgagttatc
    59461 catgctgtac gatctgaatc tagtagtcac gtgccgaatc aattggacct tcgaggaaaa
    59521 cgatacgaag aagcgctaag tgaggtagat caatatttgg attctgccat tctagctggt
    59581 tatcctcaag tgactattgt ccacggaaaa ggaacaggcg cacttcgcaa gggaattact
    59641 gattatctta aaaaccatcg gagcgtcaaa agctttgagt ttgcaccggc aaaccaaggc
    59701 ggaaatggtg cgacaattgt taaatttaag taaagcttac taaaggtaag cagatggttc
    59761 gctatccaag tatagaaatg ctatactaat catagatttt aaaaatggag gtctttgata
    59821 tgtcacaagt aattacagat aagacattta atgaagaaac agataaagga cttgtattga
    59881 ttgatttttg ggcaacttgg tgtggtccat gtcgtatgca agcaccgatt ttggatcagt
    59941 tggaacaaga atatgatgaa gaagaattca gaattgctaa aatggatgtg gatgaaaatc
    60001 cagaaactcc tcaacaattc ggtatcatga gcattccaac attaatgttg aaaaaagatg
    60061 gtcaagtagt agaaaaagct gttggtgttc attcaaaaga acaactacgt caaatgatcg
    60121 atcaatattt ataaaaactt acagcaaaaa taagctgagg gtgggactga atccagttcc
    60181 gtcctcggtt tttttgcggt tgagttaagg ttattcaaat taatattttt tgagtataat
    60241 tacgttgtca ggctggattg tttttttgac agacatgcta taataacttt aattgaatat
    60301 gaaggggagt accagatgaa cgaacgaatc aaaaacaaat tagctttact tcctgatcag
    60361 cccggctgtt acttgatgaa agataaaaat ggaacgatca tttatgtcgg caaagccaaa
    60421 atattaaaaa atcgtgtccg ctcatatttt actggcagcc ataatacgaa aacggaacgg
    60481 cttgttagtg aaatcgaaga ctttgaatac atcgttacag aatcgaatat cgaagcattg
    60541 ctcttagaga tcaatctgat aaagaaaaat gatcctaaat acaacattat gttaaaggat
    60601 gataaaacct atcctttcct taagataacg aatgaaaaat atccccgttt ggtgattact
    60661 cgtaaagtac taaaagacaa agcactgtat tttggaccat atcccgatgt cggtgcggcg
    60721 aatgagacaa agaagatcct cgatcgatta tttcctctga gaaaatgcaa acctagtcag
    60781 acgaaggagc cttgtcttta ctatcatttg ggacaatgtc tgtgtcctta ttatttcgac
    60841 gtagatccga aagtctatac gaaaatcgtt gatgaagtga aaaagttttt aaacggagga
    60901 cacgaaaaaa tcgagtcaga gatccatcag aaaatggaaa aagctgctga aaacatggag
    60961 tttgaaaaag ctgcagaata ccgagaccag atccgtgcca ttgagacaat catgactcgc
    61021 caaaaaatga caaatactga tttactggat cgagatgtat ttggttatgc gattgacaaa
    61081 ggatggatgt gtgtccaagt gtttttcgta agacaaggaa agctaattga acgagatgtt
    61141 tcaatgtttc ctttctataa cgaagcagaa gaagatttct tgacttacct cggtcagttt
    61201 tatcaggaaa atgagcattt cattcctaaa gaagtcttga ttcctgatac gatcgacaag
    61261 cccagtgtag aagctttgtt agctacgaaa gtgctgcaac caaaacgagg agaaaagaaa
    61321 aaattagttg aattggcgaa taagaatgcc tcagttgctt tgaatgaacg gtttgatctg
    61381 attgccagaa aacaggaacg gacgatcgga gcagttgaac gtttaggaaa cgcgatgaat
    61441 atcccgaccc caatacgtat cgagtctttt gataactcta atatcatggg gacgaatcct
    61501 gtggctgcga tggtcgtatt cattgatgga aaaccggcaa gaaaagaata tcggaaatat
    61561 aaaataaaaa cagtcaacgg accggatgat tatgcatcaa tgagagaagt catctatcgc
    61621 agatattcac gagtgattcg cgaagggttg cctttacctg atttgatctt gatcgatgga
    61681 ggaaaaggac aagtagatgt ggcaaaagat gtcttagaaa atcaattagg aatcgatatt
    61741 cctattgctg gcatggcaaa aaacgataaa cataaaacaa atgagttgct attcggtata
    61801 gatttggctg tagtaccgct tgagcgaaat tcaccagagt ttttcttact ccagcgaatc
    61861 caagatgaag tccatcgatt cgcaatcact ttccatcggc agctacgcag taaaaacagt
    61921 tttgcttccc gattagatga aattgaaggg ttaggaccaa aacgaaaaaa aatgctgctt
    61981 aaagagttca aatcgttgaa aaatatcaca gcagccacga cagaagaatt acaagaaatc
    62041 ggactgccga aaaatgtcgc acaaaacgta tttgataaat tacatcagga atgaggaaag
    62101 ggcactttac aaagaaagta aagtgtccta tcattttatg tacaacaaaa agagcgttta
    62161 cgttttgttg gtaaataata aatcgcttac gtctggtgtc taaggatata tgtgatttct
    62221 tgaagaagga gtgaagaaat gttagaagtc aaggatcttg ttaaaacttt tgggaactac
    62281 acagctgtcg atcatgtctc tttccaaatc cccgatggaa aaatcatggg gctaattggc
    62341 caaaacgggg ctgggaagac aacgactttt cgtttgatct tggatttttt gacacaagac
    62401 caaggagaag tgttatggaa tggccatcat cttggggaga aagattatga tgtcatcggt
    62461 tatcttccgg aggaacgggg actttatccg aaagtaacga ttcaagacca actgctttat
    62521 tttgcagaac ttagagggaa atcaagaaaa gagattgaac caaaaatcga tttttggatg
    62581 gaaaaatttc aggtaaaagg gaaaaagacg gataaagtaa aatctttgtc taaaggaaat
    62641 cagcaaaaag tccagctgat cgccacgtta atccatgaac ctaaattgat cattttggat
    62701 gagccattca gcgggttaga tccggtaaat gctgaattgc taaaagatgg aatattggaa
    62761 ttgaaaaaac atggttcatg tgtcattttt tctagccaca atatggataa tgtagaaaaa
    62821 atctgcgatc atttgatcat gttgcgaaac ggaaaaatgg ttttgaatgg aaaagtacat
    62881 gagattcgtg aatcatttgg ccgtacgaag ttattcttgg aatctggttt aagccaacag
    62941 gaagtagaag aaatcgaagg cgtgaaaaaa gtgatccttc atgaagacaa tacgttagag
    63001 attaccttag aagatcctga agctgggaaa gaaatcttta ctcgtgcgac tcagttcggc
    63061 tacattccta tgtttaacca gcaaccgcca actttggaag agattttcaa attgaaagca
    63121 ggtgaaatcg atgcgtaagt tttggattat taccaaagat gtctatttaa aaaatgttaa
    63181 gtcgatttct ttcttgatca tgatattagt cccttttatc atgctgggta tcatttatgt
    63241 agcaggaaac tttgctcaac aaaatagtga tgtcaataaa atcggcgtat tggctgataa
    63301 tgagcaaatc agtcagcaaa tgagccaagt caaaacagat gattttgagt tcaaagcagt
    63361 tgattctgaa gaaaaagcac aaaaagagct ttcggaagaa aaaatcgatg cgtatttagt
    63421 agtctcaact gagaatgata agataaatgg gatgctttat agcgaaaatt cattaggaca
    63481 aagtacacaa ctactgatac aacaacaact gactggcttg caatcgatgc ttcgagcaag
    63541 tcaattaggc atttcaccag aagaagtagc tagcttgagc caacaagcaa gtttgactcg
    63601 acaaaaagtc aattttgatt caaaaggaaa aatgacagtt ggagaagata actcgacagt
    63661 ccaatatgtc gtaagttatg tagcaacgat tgtcttgttc attattattt tgacgtatgc
    63721 gcagatcatt gcacaagaga tcgcttctga aaaaggcacg cggatcatgg aagtgatcct
    63781 gtccagtacg actgcacaaa agcattttta cggaaaacta acaggtgttt tacttgttgc
    63841 attaacgcaa atggcattat acggtattat ttttggtata ggatacagtc aattcaaaga
    63901 tatggaaatg gtcaaaaata tattagacgg tatctcattg gataacttga ttggaccatt
    63961 cttgtggtat tctcttctat tcatgatctt tggcattttg attttttctg ttttagcagc
    64021 tttatgtggc tctttggtca acaaagcaga agatacagca aaagcaattt tgccagtgac
    64081 ttatctatct ctaggcggat atatgcttgg attgatacta ggagcttcag atccaaacaa
    64141 tatcgttatt cggatcactt cgtatattcc attcttatct tcatatatta tgccggttcg
    64201 cttagcaaat gagacagtcg gtatgggtgg agcattgatt tccttgttgg tacttgtagc
    64261 cgtaacattc ttattgatga tcttgtctgc aaacatgtac aaatcgaatg tactcgtgta
    64321 tagcgaaggc ggattatgga gttcattgaa acaatcgatt tcaattatga gaaatgaacg
    64381 aaaaaaagca taaaatatag aaaaatatag aaggaagctg gacatttatt gttcccagct
    64441 tcttttttgt atagacctat ttctattttt ttattatatt ggatatatat attcattgaa
    64501 aatagagcat aacaacttta taatgttgag tataccaatt tttggaggcg ttttaatgga
    64561 ccgctattta gatttacttt cagagaagtt ccctacaacg gaagatgtca tcacggaaat
    64621 catcaatctc gaagcaattt tgcagcttcc aaaaggaaca gagttatttt tgagcgatat
    64681 acatggcgaa tttcctgctt ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga
    64741 aaagatcaaa gaccttttct ctgaacaaat gacggaaacg gagatcagcc aattcactat
    64801 ttttacggct tatccagaat atgtgttgac gactgattgg tacaaaaaac aagataaatc
    64861 ccagctgatc catcgtttga tcgatctttt acggtttacg acagtcaaat acactcgatc
    64921 aaaagttcga aaaggattgc cgaaagaata tagctatatc attgaggaac tcttgtatat
    64981 cgatgatcgt atcaacggga aaaaagacta tgtaaaaaaa atcattgaac aacttgtctt
    65041 gatgaaggaa gaagaaaaat ttttaacaaa acttgcgcag acgatccaaa aatctgtcat
    65101 cgatcatttg catatcgtgg gtgatatttt tgatcgagga actcaagcag ccaaagtaat
    65161 ggatcgaatc atggattttt cctctgtaga tatccagtgg gggaatcatg atattttgtg
    65221 gattggtgcg tattgcggct ctgaggcatg tttattgacg cttttacgga ttgccgcacg
    65281 ctacaactat ttatttgaat tagagaaaga atatggatta aatttacgtc cattattttc
    65341 ttttgcccat caaacctatc agaagaatcc agtatttacc cctaaaaaca ggaaagatct
    65401 atctgaaaga gagcaagaag aattagaaaa gatccatcaa gcactttcta tcctccaatt
    65461 caaatcagaa catcagttgt tgaatcgacg acctgaattt aaaatggacg atcgtaaatt
    65521 gttggaaaaa atcaactatg aggaatcaac gatcgtttta gaaggtcagc tctatgagtt
    65581 gaaagggact tgttttcaaa cgatccagcc agatgatcct aaaaaattct tggaagagga
    65641 acaaaaagtg atcgatagtt tgatgtattc attccagcac tcgttaagaa tgcaaaaaca
    65701 catgacgttt ttgatcgaaa aagggggaat gtatctcacg aacaatcagc atgtactgtt
    65761 ccatggctgt atacctgtag atgaaaaggg gcagctctta gcatttgaat tagaccaatc
    65821 ttatcgcgga aaagaactgc tgggattttt tgaaaaacag ataactgaaa gcgcaaaaga
    65881 tttgactgca aatgaagatt tggcgaccga tttgatttgg tatgcgtgga gcgggccatt
    65941 ttcgccttta ttcggcaaga gtaaaatggc aacttttgag cgctattttc tcacagagcc
    66001 tcatacgcac gtcgaaaaaa gcaatcccta ttatcacttg cgagatgaag agtggttcag
    66061 tgaaaaaatt ttggcagaat tcgatgcaaa agaagaaggt tcagcaatca tcaatggaca
    66121 cacgccagta aaagtaaaaa aaggagagtc accgatcaaa gcaaacggaa aagcatttgt
    66181 tattgacgga ggtctatcca aagcttatca aaaaacaacc gggattgctg gctactcatt
    66241 actatgcaat tcttacggtt tccaaatcgt gacacattat ccgttcttgc ctattgaaca
    66301 acaatttgca aaaaaatctg atcaaacgaa tgtaaaaaaa gtgatcgagc agcagctccc
    66361 acgcaaactt aatcggaata cgacaaaagg aatggaatta cagcaacaga ttactcaact
    66421 ccaaaggctg ttggattatc gaaaggacga ttgagaaaaa tgctagccaa cattgctcaa
    66481 tagactaaaa agcgactgca acaaaaggcg tgtcgcagtc tccttcggaa ttagctcaaa
    66541 aaacggtaaa atattagaag ctatttttct gtttatttct ctaatactca ggtcagggcg
    66601 ctgatttcac gacctcttcc ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca
    66661 caatatccaa caaacatgag aagctgtttt ttgaaattgc tccttgtgac tcagagttga
    66721 acggcaggct cacgacctaa tcagaaaaac gattaaaatt cattcacaaa gaaatcgaat
    66781 aaacgctgtt cagatgaatc tacacgatga gaaagttccg gatgccattg gacaccaagg
    66841 attttgtgca tattatcttt actttgtact gcttcaatga gtccatcgtt ggaaaaggcg
    66901 atggctttta acaaaggaga aagatctttg actgcctgat gatgatagga attcacaaag
    66961 tatgaatcag ggagaaggtc acttaataaa ctgtccgaaa cgattttgac ttcgtgagtt
    67021 gcaaactgcg gggcagtggg ctgttgttcg tgttttacgg accattttgg atataatgaa
    67081 agatcttgat aaagtgttcc ctccaacacg acattcaata gttgaagtcc tcggcagact
    67141 gcaaaaatcg gttttctttg tttcaatgct tccaagatca atgcctgttc gaattggtct
    67201 cgttgaatat ttgtttccgt cagttttgga tgagggtctt ccatgtaaag ttgcggacta
    67261 acatcctgac cgcctgcaag aatgagtttg tcgattttct caattagttg aggcgctgaa
    67321 tctggagaca tgactggcag aataagaggt aatccttgag catctaaaac tgcatccaca
    67381 aatccctgcg gtgtatagct tacttgattt ccttggaaag tatctgtcgc tcgaatcagt
    67441 tgattcccgg caatgccgat gattggtttc atttttattc cccctaaagt agactaattg
    67501 taacaaggat ttaaaaaaat ttaaactatg taagacttgc ccctatactt atctgttctt
    67561 gctgtgttac tatctgtaaa gagaattttc gtgtgaaatt catgacgata gttgaaaata
    67621 ttgcttaatt tgttataata aaaagtaagc agaaataaaa aggttgggcg taagcttcaa
    67681 ggatctagtt cttcaagggg tgagaaggac attttattga aggggcctgc ctatactaag
    67741 ttatctttag aggtacatac tttcaatgaa aaaagaaagc atgtacctct tttttaaatc
    67801 gaaaggaagt gaagcaagtg ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa
    67861 atgagaagcg tgctcgaacc ctacggcagc agttgaagac ctatgcagaa gaaaaagagc
    67921 aagaattgga gataatccaa acgaacagtc ggcaacagct gaaacagttg gtagaagaaa
    67981 aagaacaatt cgaaaagcaa gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag
    68041 acaaggtgca agcaactttg aaagaccaat atcaaaagca tcagagtcaa gcaatcggtg
    68101 caatcattga aagggtgaaa gaaacttatg gccgtcacta agatggaaaa agtgaccctc
    68161 atttcagata aaagaaatca ggagaggatt ttacaagcag tacaaggaat ccaaaatgtt
    68221 gaaatccggg atctgtatca aaatagaaca aacaaccagt gggtagaaaa atattttcca
    68281 gataccacga ttattgataa agaagcaaaa ttaagcgctt taggcaatcg gctgaaagac
    68341 atccaagaag ctatccaatt catacaacac cacggcgata gccgtcaaaa gaaactccat
    68401 ttgaaacgtc aagaactttc cttacaagac ttggaagcaa attattccga agaaaatttc
    68461 atgaataaat tagaagaagt ccttgattta aaagaaaagt gggaacagct aactgaaaaa
    68521 cgtgatcgtt tagaagaaga ggaagaatgg cttcttcatt ggcaacagct ggatattgct
    68581 ccaagtatgt atgcaagtaa gacgaccatt tttgagatgg ggactgtgag tgcagcaaat
    68641 tttgaaagtt tcaaggaaga actaagtaag cttgatgacg tttatatgga agaaatagat
    68701 tatacgccaa aacaagttta tctttcgtat gttgctttaa aaaaatcagt tgctgctata
    68761 gaagaaattg ctgggaaata cgggttcaat aaagaaacgt atccttatga acaaaatcct
    68821 aaagaaaaat tgcaagaaac caaacagcaa ttatccattg ttcacgagca gcaaaaaaag
    68881 ataacgacag cgttaggtca gtgcagcggc tatatccaag aatttgaatg ggtagaagaa
    68941 gtcacacttg ccgttgcaga acgggaaaaa gtaaaagaac ggtttatcca tgccacttat
    69001 ctgatcgttt tgcaaggttg ggtagatgca gaagaaaaac agaatttgat gcatgtgtta
    69061 aacgaaactg tatcagaaga agatatatac gttagtttcg aaacaccaaa ctcagaagag
    69121 attcagcagg aagtccctac taaattgaaa aaccatccgc ttatcgaacc ttttgaattg
    69181 ctgactgaaa tgtacagtct gccaaaatat gaagaagtag atccaacgcc ttggatgaca
    69241 ccattttatc ttgtcttctt cgggatgatg gtcgcagatg ttggttacgg attgctgatg
    69301 ctcattggga caatactagc tcaaaaacta tttgttttac cccgcgggat gaaacgtttc
    69361 gtaaaattct ttgagattct ctcggtacca tcgatcatct ggggattgat ctacagttca
    69421 tttttcggac aggcattacc aaaagaacta tttggtatcc gcttgccttt cccaatccta
    69481 tctacgacgg atgacgtcaa tacgattttg attttatccg tgattttcgg tctgatacag
    69541 atcttggttg gattattcgt ggcagcaaag gaaaatatca ggaggaaaga ttacttgagt
    69601 gctgtcagtg atggttttgc ctggcaaggg atcctgattg ggatcgttat tgcattgatt
    69661 ggtgtcatgt tgataaaaaa tacagcattt gtttatcttg gcgcggggct atcgatcatt
    69721 gctgctttat gtatcttaat cgtgccgatt atccaatcgc cgtctaaagt caagggtgct
    69781 gccaaaggag cgtataatct ttacggattg actggttata tcggtgattt agtcagttat
    69841 acacgtttga tggcactagg tatttcaggc ggaagtattg gcgcggcatt caatatgcta
    69901 gttgctttca tgccgccagc cgctcgattc agtgtaggga tccttttgat catcgcttta
    69961 cacgcactga atatgttttt gacactgtta agtgcctatg tccatggtgc acgattgcag
    70021 tatgtcgaat tttttggaaa attttataca ggaggcggac gtgcctttga cccgctgaaa
    70081 acggcggaaa aatacgtcaa catcaatcat aagaaaaaaa acaataaata attggaggat
    70141 ttttatacaa tgatggatta cttaattagt caaaatggtg gaatggtatt tgcagtatta
    70201 gcgatggcaa cagcgacgat tttttcaggg attggttcag ctaaaggtgt tggaatgaca
    70261 ggtgaggcag cagcagcgtt aacaacaagt caacccgaaa aattcggaca ggctttgatt
    70321 ctgcaattgc ttccaggtac gcaagggttg tatggattcg ttatcgcctt tttgattttt
    70381 atcaacttag gaaatgatat gtcagttgta caaggattga actttctagg cgcttctctg
    70441 ccgattgctt ttacaggttt gttttcaggt atcgcccaag ggaaagttgc agcagcaggt
    70501 atccaaattt tagcgaaaaa acctgaacat gctacaaaag gaatcatttt tgctgcgatg
    70561 gttgaaacat atgcgatttt aggtttcgtt atttccttcc tattagtatt gaatgcctaa
    70621 gcataaagga ggaaaagcag atgaacgcca ttgaaaaaat catctctcag atgaatgaag
    70681 cagcagagca agaaagagcg gcgttagagc aagaagagcg aatgaagatc gatcaaaact
    70741 ttgaacaaaa acgtacgcaa atcgaaacag aacatcaaaa acaaaaagaa aaacaaatcg
    70801 aattacttga aaaaaaatac cgtcagctta ggaatcgcca acaagtagaa gtacgacaag
    70861 aaaatttgaa tgcgaagcag gaatttttac gacgattgtt tgcagatgca atagctgaaa
    70921 tggagaattg ggatgaatct gaacaaatcc agtttatcaa aaattcattg tattcattac
    70981 ctttgacagg caaagtaaca ttcattgctg gagaaaaatc agcagcttat ctttctcaga
    71041 cattgctgga tgaatggaac aatgaacttc cctttatgat ggttttaagt gatgaaactg
    71101 ttgctgatca agcgggtttt ctgataaacg atcaaggagt tcagtataat tttcttttca
    71161 gcagccttgt acaagatatc caaggaacga tgagttttga gattgccaat caattattcg
    71221 aataaggagg gatcagtgta tggaatacca cgaactaaat cccttgattc gcggaagaga
    71281 gcttgaactt ttgtccaatg agacatttga acggatgatt caaacaaatt ctgtcgaagc
    71341 acttggtgaa atattgaaat ccacgattta cagtccatat atttatgacg gatatgaata
    71401 tgacttcgaa cagaatcttt taaaggagca gagccgtcta tttcgctggt taaaggaatc
    71461 agcaccggag cctgaaatcg tttggatcta tacgatgcgc tatactttcc ataatctgaa
    71521 ggttttgaca aaagccgaga tgacaaatca aaatctggac catttgtata tcgacgatgg
    71581 attttattca ttagaaacct tgaaggatgc cattcatacg caagtttcaa atgaactccc
    71641 tgaaagcatt atggacagta tacgagaagt acgtgattat tttgaagagt ctaccattct
    71701 tcagggaatt gacgtgattt atgatcgaca gtttttgaca gagcagcgac ggcttgccga
    71761 taagctagga tatcaagaat tgctgacaga agtcattgct tttattgact tgaccaatat
    71821 cacaacgatg gctcgaggaa tattgcagaa aagaacatat gggttcatga cgacggtact
    71881 ttccagctcg ggaagtatcg aaaaagaagt atttttgaac tttgtagata aagatatgga
    71941 agattacacg aattttctat tagagacaga ttatgctgat tttctgcgcc ctgtcatccg
    72001 tgagggaaat cttgatctcg taaagcttga acagttgaaa gatgattatc taagttcatt
    72061 gtatcaagaa gcacaaaccc aggctttcgg accactgcca ctactagcct ttttgaacgc
    72121 aaaagaggta gaaagcaaaa accttcgttt actggttatc ggcaaaagaa gccatttcga
    72181 accagaaaca atcagaaagc gggtgagaca agtctatgac gcataagatc ggtgtagttg
    72241 gcgacaaaga ttctgttttg ccattcaaat tgtttggctt tgatgttcgt tatggtacaa
    72301 ccaagcaaac cgttcgtcag gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt
    72361 atgtcacaga gcaatgtgca gcattgagcc cagaaacgat cgaacgttac aaggaacaaa
    72421 tgatacctgc tatcgtattg attcccaatc atcaaggatc attagggata gggctggaag
    72481 aaattcaaaa aaacgtagaa aaagcagtag gtcaaaatat cttatagtat gaaaaggaga
    72541 tagaaatttt gcaaattgga aaaatcataa aagtctccgg acctcttgta atggctgaga
    72601 acatgtctga tgccagtatc caagacatgt gtctagtggg agatctagga gttatcggag
    72661 aaattatcga gatgcgtggc gatgtggcgt ctattcaagt atatgaagag acttctggaa
    72721 ttggtccagg cgaaccggtt cgctctactg gtgaagcttt atctgttgaa ttaggaccag
    72781 ggatcatttc acaaatgttt gacggaatcc aacggccatt agataccttt atggaaatca
    72841 cacaaagtaa tttcttaggg cgcggagttc aattgcccgc attggaccat gagaaaaaat
    72901 ggtggttcga accaacagta gaagccggag aaacggtgag tgctggagat gttatcggga
    72961 tcgtagaaga aacaaaagtt ataaaacata aaatcatggt gccaaatggg ataaaaggaa
    73021 cgatcaaaac aatcgaaaaa ggttcattca ctattgatga aacagtatgt gtcattgaaa
    73081 ccgaaaatgg tgacaaaaat ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc
    73141 caatcaaaga aaaattaaac ccggatgcac ccatgatcac tggacagcga gtgattgata
    73201 cattcttccc agtgacaaaa ggaggagcag cagctgtacc tggtcctttt ggtgcaggga
    73261 aaacagtcgt tcagcatcaa attgcaaaat gggccgatgt ggacatggtt gtctatgtgg
    73321 gatgtggcga gcgtggaaat gagatgacgg atgttttaaa tgaattccct gaattgattg
    73381 accctaatac aggagaatcg ttgatggaac gtacggtatt gattgctaat acatcaaaca
    73441 tgccagtagc agctcgagaa gcttctatct atacgggaat cacaattgcg gaatatttcc
    73501 gtgacatggg ttatgacgtt gctatcatgg cagactccac ttcacgatgg gcagaagcac
    73561 ttcgtgagat gagcggacgt ttggaagaaa tgcctggtga tgaaggttat ccagcttatc
    73621 ttggttcacg gcttgcggaa tactatgaac gatctggaag agtcattgca ttaggatcag
    73681 aaggtcgcga aggaagtata accgcaatca gtgctgtatc cccgtctgga ggggatattt
    73741 cggaaccagt cacacaaaat acattgcggg tcgtaaaagt attctggggt cttgattcca
    73801 gtttagccca aaaaagacat tttccttcga tcgattggat acaaagctat tctctttatt
    73861 caacagaagt agggaagtat atggatcata tccttcaagc tgactgggca tccatggtca
    73921 ccgagggcat gcgtattttg caggaagaac agcagctgaa tgaaatcgtg cgtttggtag
    73981 ggattgattc cctttcagat aatgaccgtc tgacattaga agtggcaaaa tccattcgtg
    74041 aagattattt gcagcaaaat gcttttgatg atgtcgatac attcacttca agagagaaac
    74101 aattcaaaat gttgaagatg atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt
    74161 taggtgctta tttaagtgag atcatggacg gtaccgtatc gattcgtgaa aggatcggac
    74221 gaagcaaata tatcccggaa acagaattag aaaaactaga tcaaattatt ggtgaaatca
    74281 agcaaacgat gcaaacgatc gtttcagaag gaggcatgag caatgattaa agaatatcgt
    74341 acgatcggtg aagtcgtcgg tccattgatg gcggttgaaa aagttgctgg tgtcaaatat
    74401 gaagaattaa tcgaagtccg tatgcaaaat ggcgagattc gtcgtggaca agtattggaa
    74461 gtccaagaag ataaagcaat ggtccaaata ttcgaaggaa caagtggtat caatttacgt
    74521 gactcttctg tacgattttt aggccatcct ttagaactag gtgtatcgga agacatgatc
    74581 ggtcgagtat tcgatggttt gggacgaccg aaagataacg gaccagagat cctgcctgaa
    74641 aagcatttgg atattaacgg agaagtaatc aatccagtag ctcgggatta tccagatgag
    74701 tttatccaaa caggtatctc tgcaatcgat catttgaata cattagttcg tgggcaaaaa
    74761 ttaccagtat tttctggctc tggtcttcct cataaagaat tagctgctca gatcgcgcga
    74821 caagcaacgg tactcgattc atcagatgat ttcgccgtag tctttgctgc tattggaatc
    74881 acttttgaag aagctgaatt tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt
    74941 tctgtcatgt ttatgaattt agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt
    75001 atggctttga ctgctgcaga atatcttgct tatgaaaagg gaatgcatgt tttggttatc
    75061 atgacagaca tgaccaacta tgcagaggca ttgcgagaga tttctgcagc acggcgagaa
    75121 gttccaggaa gaagaggata tcctgggtat ctttacacga acttagctac tttattcgaa
    75181 cgagcaggaa gaatccgcgg actaaaagga tcggttactc aaattccgat tctaacaatg
    75241 ccagaagacg ataaaactca tccaatccct gacttgactg gttatattac agaaggacag
    75301 atcatcttga ctagagagct ttacaagagt gggattcagc caccaattga tgtactcccg
    75361 tctctttcgc ggttaaaaga taagggaact ggacccggaa agacaagaga agaccatgca
    75421 gctacgatga atcagctttt tgcagcctat gcacaaggaa aacaggcaaa agaattagcc
    75481 gtagttttag gtgaatctgc tttatctgat gtagacaaga tctatgcgaa gtttgctgag
    75541 cggtttgaaa aagaatatgt caatcaagga ttctacacga accgttcgat tactgaaaca
    75601 cttgatctag ggtgggaact gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa
    75661 gacgacatgc tggacaaata tctgccagaa gggaagtgag ttctgatggc acgattgaat
    75721 gtgaatccga cccgcatgga attgactcga ttgaaaaaac agttgacaac tgccacaaga
    75781 gggcataaat tattaaaaga taagcaagat gaactgatgc gtcaattcat cctgttgatt
    75841 cgaaagaaca accagctgcg tcaagaaatg gaagagtcta tgacagaagc aatgtctgat
    75901 tttgtattgg caaatgcttc gatcacggaa cctttcatcg aagaattatt tgttttgccg
    75961 gcggaaaacg ttgatttgtc tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg
    76021 atgaattttg aatatgatga acagctgaca gacacacctt tggaatacgg gtatttgaat
    76081 tccagtgccg aactggatcg gtcaatcgat cggtttacta ccctgcttcc aaaacttttg
    76141 gaactgacag aaatcgaaaa aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt
    76201 cggcgagtca atgcattaga atatatgacg atccctcaat tagaagaaac gatctattat
    76261 attcgtatga agctggaaga aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa
    76321 aatatgggta ccactgacta agtggtatct ttttttaaaa gaggaaaacg atgattaaac
    76381 aaagcaaaca aataaagaaa cgtttgccgg ctgttcagct tctcgcatta ggattttttc
    76441 tcctgatttt agttggcggc ggcctactta ccctaccatt ttttagtaat agtgggcatg
    76501 ggacaaattt tatcgatgcg cttttcactg caacttctgc tgtctgtgta acgggattga
    76561 caacattgaa tacagcagag cattggaaca gtgctggtca atttttgatc atgcttttga
    76621 ttgagatcgg cggtcttggt tttatgatgg ttccgattct atgctttgca ttgataaaga
    76681 agaaagtcag tctcagcaca cgtattatat taaaagaagc gctgaatcta gaagaagtat
    76741 caggtgtgat gaagctcatg ctttacatcc taaaattggc ctttgttatc caattgattg
    76801 gtacggttgc tttatctttt gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt
    76861 acagtttgtt ccatgcaatc tctagttttt gtaatgcagg gtttgatttg ttaggagaca
    76921 gtttagccaa tcaccagcaa aatgtttatt tgatcatggt ggtatctgct ttgatcattt
    76981 ctggtgggtt aggcttcatc gtatggcgtg atttactgga atatcatcag aaaaagaaga
    77041 tgacgattca ttcaaaaatc gcattaaccg ttacaggtac ccttttatta ggcggattcg
    77101 ttgtgttctt tttcacagag cgcaatgggg caacgctagt ggaagggaat atgtttcaac
    77161 gattggcaaa cacctttttt atgagtgtga cacctagaac ggctggatat tattcagttg
    77221 attacctcca tatgagccat gcaggtctga ttttaacgat gtttttgatg tacatcggcg
    77281 gaacatctgg gtcgacggcc ggcggattga aaacaactac gttaggtgtg ttgctgatcc
    77341 agatgcgttc gatgttgaaa ggacgtacga gagcggaagc atttggccgt accatccgac
    77401 aaggcgcagt gttgcgggca cttactttat tttttgttac gttgacgttg tgtgtagtcg
    77461 ccatcatgat tttatcggtg actgaaacga tacctaaaac ttctggcatc gaatatattg
    77521 cttttgaggt cttctctgcg tttggtacag taggtcttac gatgggattg acgccagact
    77581 tgactgcaat agggaaattg atcattatag cgcttatgta cattggacga gttggtgtat
    77641 tgactgtggt cttctctctg atgacaaaag cacaaagaac acaatcaaaa tacaaatatc
    77701 cagaagaaag tgtcatgatt ggataatttc atcttctgac agaaacctag tgatttagta
    77761 aaataagaaa ataaaataat ttttcttgct tcttggatac tttaagtgca aaatgttcgt
    77821 ttattttttg aggcactacc cgttgttgaa cctcgggtag tgcctctttt ttattgaaag
    77881 gaatcaataa gatttgattc ctttatttat caataggtta taattcatat ttttttattt
    77941 tttcttttac gatacttagt ccagaagcta aaatttcaag gttattagag gatagggaaa
    78001 ttcgaacgaa tgcatctggt tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga
    78061 taccgttagc aagaaagtca ttctcaatta tcttagggtc aatctcagga tcaactggaa
    78121 gaattcgata gtaagggaca cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa
    78181 gctgattggc tctttttgta agacttaatt tagtttctaa gatcctattt gcttcatttg
    78241 aatggaggat ttgagtgatg acttccacat ctaatccaga agttttcaca tttatattaa
    78301 aaattgcttg ttctatcttg ctttggacat gtactggaac tacaagataa gcaactcgca
    78361 atccagaaca aataaatttt gtcattccag ctaagtagat tgtttggtta ggtaataaat
    78421 tttggaaagg aggaatgatc gtatgttgat agtaagttgt taaaaatgaa tggatatcat
    78481 cttcaataac ccatagacct tctttttcaa tcacttcggc taattgctct cgtcttttgt
    78541 gagagatttg aaaccctata ggattattac aagaaggcat aagaaagaca ccatgaatct
    78601 tcttttttct acattcctga atcaatatat caggtcgcat tccttgctcg tcaaaatcaa
    78661 ttggaacgat ctctaaatga tataactgag ccaattcaat gaaattagcg tatgtatatc
    78721 ggtctgttgc tattctatca cctggagaga aaagtgcaga aagtgtgata gccaatccat
    78781 tttgaacacc ggaaacaata gatagagatt ctaaggacgg aatgactcct tgagttttta
    78841 accaatttgc tgcagtagtc aattgaaaat ttgtgccttt gggatcttgg taattaagca
    78901 aaccaacaag atttgggtta ttactagttg attgaataaa cgggacaacg aatttattac
    78961 attcttcaaa agaactaacc agaccaaaat caatgatatg ttcggttact tcattggtag
    79021 agatggtgat ggactcaaag acattgggag aaataaatgt gccacttcca atattcgtat
    79081 acagcaaacc tttctcaatt ccatatttat aagccttgcc aacggtagta aaatttaaat
    79141 ctaaataatc agctagctct ctttgggagg gtaactgagt gtttttctgt aatttattct
    79201 ttaaaatatc ttgttctaat tgttgaatca acgatcgata ataaggacgc ttcaattttt
    79261 gtttgtctgg tcgccaagtt aacgggtaat tgtcataaga attaataggc attttctcca
    79321 ctccttaaaa ttgtatacca tacaatttta ccattgattg tattgatttt agaagttaat
    79381 cttttgtgag gagggaattg tctatgaaaa ttattaattt tgaggaagta gtagcttctt
    79441 ttagttttga agaagcgatt caagtaatga aaaaatgttt ttttgattat gaaagtggta
    79501 aaatcagcca aaatcctcga ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt
    79561 ttgcgatgat gcctgcttat ttgggggaga acagatattt tggcacaaaa gtcattaccg
    79621 cttttccgga taatcatcag tatcatttac cttctcattt aggagagatt ttcttgtttg
    79681 atgctcaaaa cggactaccc aaagcattga taaacgcaaa tgcagtcacc tggattcgaa
    79741 cagctgctgt atctgcgctg gcaacagatt acttagcaaa tccgcatgca gaaacacttg
    79801 ctttaattgg agctggtcaa caagcattgt cgcatttgac tgcgatttct accattcgtc
    79861 ccatcactac agtgtttgtt tacgatattt tagaagaaag acgaaatact tttatcgcta
    79921 aagctaaaaa acagtatcct aaaataacgt ttataaatgc aatatcagta gaagatgcag
    79981 tacgtccagc ggaaatcatt tgtacgttga cctctagtaa agaggcattt cttcaaaaag
    80041 agtgggtaag ggaagacgca catatcaatg ctgtgggcac atttactcct gatacaagag
    80101 agataagtac atcgttgatg gaaacttctc aaatctatgt agatgattac tcagctgcct
    80161 taacagaaag cggggacctg cttatagcca tggcagaagg agatttagag gaatcttgta
    80221 ttgttggtac attaggcgaa ttagtttctg agaaaaaaac attttataaa ggagaatcga
    80281 ggccgacgat tttcgacgca gttggtctag ctattgaaga tctttgttgt gcagaatata
    80341 tttataacaa attagagaaa ggggctttaa aatgaactta gattattacg tagttgatgc
    80401 atttgcagat gaggtgttca aaggaaatcc agcagcggtt tatgtgctag aagaatggct
    80461 gccagaaggt acgatgcaaa aaattgccat tgaaaacaat ttatctgaaa cagcatttac
    80521 agtgaagaaa aaccaagaat ttgagcttag atggtttacg ccagatcgag aaatcgatct
    80581 ttgtggtcac gcgacattag caacagcatt tgttttattt aactattaca aaatacctga
    80641 tgaaacgatt aaattttcta ctcaaagcgg taatctattt gtgacaagaa ttaaagatta
    80701 ttattacatg gattttccta gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga
    80761 agaagcgatt ggggctaaga ttaaagaagc ctacttagca cgtgatttat ttttcgtatt
    80821 agaagatgaa aaaacggtgg ctaaattgca gccagatttt acagctatca aaaattttga
    80881 attaggaata ggagtcatcg tgacagcaga aagtgtgcaa aaagactttg tttctagaac
    80941 cttctttcca aaattaacaa tcaatgaaga tccagtatgt ggatcagcac attctaatct
    81001 tattccttat tgggcagaaa gaatgaataa aacaaaattg tcagcttatc aaatgtcatc
    81061 aaggggcggg gatctagaat gtgaattaaa agaagatcgt gtgataatag gaggaaaagc
    81121 agttttgttt tctaaaggta cctcgtttat ttgatttctt tcaaatgaat taaaaataaa
    81181 ttggtggaga gtggcaacct tatagctaca atggttgtta aaaaaagacg caaacttttt
    81241 caatttaatg aaaagtttgc gtctcatttt aattattggt gtttgttttt tattcaatca
    81301 agagtaaaat gccccatact aactatcctc ggatgacttc gattttataa ccatcagggt
    81361 caatgacaaa gtagtaagaa ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa
    81421 gaccggcagc tttatgttgc tcgtgtaatt tttctagatc atcagctgct atagcgatat
    81481 gaccatagcc gtctcctaga tcatacgcac catgatcata gttgtaagtc aactctaatt
    81541 catagtcatc cccaggaagt gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac
    81601 ggcggcggct ttcttcaaaa ccaaatgcct tttgataaaa ttcgattgat gcttcaagat
    81661 ctttcactcg aacacatgtg tgagccattt tcatagattc atctctcctt ttccttttac
    81721 tcaatcataa caaaaagaag taaggaaata gttgatttgt gcttgttcat gagaaatttc
    81781 tcatgtaaaa tggatagaaa ataaaactgt taaggtggaa actattcatg gataaaccaa
    81841 tttttggtga aaaaaagcaa tttgctgatt atcaaacccg ttatgcagca tatattgtca
    81901 tagaaagaga tcagcagatt gcagtaatcg aggctccaaa tggtgctttt tttcttcctg
    81961 gcggggaaat tgaaggagaa gagacgaaag aagaagcgat tgtccgtgag ctgatagaag
    82021 aaatgggaat tgtggcagaa atttcttctt atctgggaga agctgatgag tatttttact
    82081 cgaattatcg tgcaacttat tattataatc caggatattt ctttgttgct gattcttgga
    82141 agcgtatagg agaaccaact gaaaagacca ataaaatttg gtgggtgagc cctcaagaag
    82201 cgcttgtgaa attgaaacgt ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa
    82261 ataaaaactg aaagaaagcg agtagatcag ctttctctca gtttttttat ttattacagt
    82321 acatattttt tgattgcttc agctacaccg tgctcgtcgt ttgatgaagt gatgacattt
    82381 gctgcttctt tgacaagaga aacggcattt cccattgcta ctcctagacc agcatattca
    82441 atcatggata agtcgttctc gttatctccg atagccatga tttcatcttg atcaatacca
    82501 aggtgctgtg cgagattcgc aactgccgca cctttacttg cttctttatt tagtacttcg
    82561 aagtaatagg gcgcactttt gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc
    82621 gcaattgctg catctaaaat ttctggttcg tcgatcatca tcattttgac gatgttcatg
    82681 tctggtgtca tttcttcagg tgttcgatat tttagcggca tcttgactaa acttgcttcg
    82741 tggatcgtat aagcgctgat atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa
    82801 tggagatgac tgccgacacg gcgtgccatc acttcgatct ctaggaaatc ttcatgtgtc
    82861 agtccgtgac gggcgatgat tttacctgat ttcgtttgtt gtacgagtgc cccgttataa
    82921 gtaataacgt aatcattatc ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct
    82981 ggtaagggac ggccagtgca taaaacgatc gaaacacctt ttgcaacagc agcggcaatc
    83041 gcttctttta cttcttttgt tacttctctt ttctcattta ataaagtacc atcgatgtcg
    83101 atagcgacta gtttgataga cataatttcc tccaatatat tttaatttat ttcaatcaat
    83161 gcgccattac ggatatggct ggcaaattct tgataagttt cgtcaaataa atcataatga
    83221 tcacgcaagg acggatcaac catttctttt ggaaagaaaa atcgctcatc tccttgttct
    83281 tgtcccgcta atgcagcaac aagctgacta acctgtgata attctatcaa agttccgtca
    83341 tttcgcaaaa tctcgatttg tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa
    83401 ggtagatcgt agctagaatt cacagcagtg taataaacag gattatatcc gaccttttct
    83461 actaacaaag tgagttcttg gactaatttt gaatcccggt tgccagaaaa agtagcagat
    83521 ttcagtggtt tacgattcaa aaaacgttta gctaaatcat ttaaaatagg atcagcagat
    83581 tcagaccatt gagtaaagta agtgcccaag accccatcat caagtttgag gtattcttct
    83641 aaagtaaagc ttccgtcaag aaatggcaga agtaattgag aatggagttc aaaactgctg
    83701 gcatcttctt gatacaactc gtgtgcgcga tgaagcagat gctccaaaat tacttccata
    83761 ccacgggaaa caggatggaa gtacacttgc acatacattt gatatcggct aacgatataa
    83821 tcttccactg catgcatgcc actcatggaa aaagcgatcc cgccttcata aggacgaatc
    83881 acacgtaata ttcgggtaag atcaaatgtt ccatattctg ttccagtgaa ataagcatcc
    83941 cgaagcaaat agtccattcg atctgcatcg atctgactag aaatcatttg gacaacttgt
    84001 gggttggggt aagttttttg gatcacacta gctacctttt caggaaaacc ttcttctact
    84061 cggttaagaa tctgatacac ttctgtttct ggagaagtga tgatctctac agtgatggct
    84121 tcatgattcg tatggaagat atgttcaaat gtatgagagt aaggaccatg accgacatca
    84181 tgcaacagtg ccgcacataa agtcaccagc cgttcctgat catcccagcc tccgttgcct
    84241 attttttctt ttgaaaaatt tcgagaaaag atatcgcaga ttcgtcttga aatctcataa
    84301 acgcctaatg aatgagtaaa acggctatgt tctgctccgt gaaaagtgaa agaagaagtg
    84361 cctaattgtt taatgcgtcg tagacgttgg acttctttcg aattgatcaa atctaagatt
    84421 acttgatgct ggacatggat atagttatgg actggatcac gaaaaacttt ttccataggc
    84481 agcatttgat atttataagg ggttgtcata atgtagctcc tttatgttat tacgttgttc
    84541 cattctctgg atttggttgt tccattgctc cagctggatt tcttttttta gccagtcggt
    84601 agaatcctgt aaatcacttg cagaatacag cttttcaaaa gcttttgcaa atgcttcttt
    84661 gacttctttt acaaaaaggg gttggtcaag cagttcttca agtgttgcca ttgtagcagg
    84721 atccactgca ggatagccat ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg
    84781 cttcatgact tgcccgcgtt tttcctgatt accgttgaca cttaagtaca tcataacaga
    84841 aatcccattt ttgatccgac gctgagcgat accggcaaat tttttgccat ggatgcttaa
    84901 atcataagtt ccaggacaat aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg
    84961 aaaagcgtat ttagtcaata ggagcatttg ctcgtaagcg tcatctatgg ttattttttt
    85021 tgtgtcactt tgggggaaaa tcaaagaaat attcaaaatt ccttcatctg atacgatccc
    85081 taatccacct gaatttctga tcactggagc gtaacctgca tctttcagtg tttggatccc
    85141 gtcacttaga aaaggagttc gaacatcctt catacctaag atgactgtgc agtcatattg
    85201 ccagaaatgt aaaaagattt gctggtgttc tttagaatat tccgttaata tatctgttaa
    85261 tgcaaaaggg gaaaagtcgt ttttttgata aatgtgctga tctagtcgaa tcgcagggtt
    85321 cattcaaccg gctcctttgt ataaattcat attttctaca atattatact ctaaaatcaa
    85381 agagttgttt tggaaaaaag gttttctgta aaaatggaca agttggttga caaaacaaaa
    85441 gaaactagga acaatgtagc tatgagaagt aaaggagaat gaataatgga attatcacaa
    85501 ggaacaccga tttcaatcaa attgcgtaca aaagtaaaac agcaagggga agtgcaagat
    85561 ttttattttg atttgaaagg gcaaatggtg acaattggcg atacgctgta catacgttat
    85621 aaagagattc aggaggagac gggcgatgag attcctgtaa cgatcaaatt agttccagat
    85681 ggtcatgtac agctgatccg tgcaggagaa atgcggatgc gtttgaaatt tggctataaa
    85741 gaacggttag aaacatccta tcgaacacca tacggtatga tccagattgc tacttttacg
    85801 aaagaactgc atgttagttt gaaagaccgt cccacaggag ggaaggttcg aatcgattat
    85861 gatctgttca tgggaccgga aaaaatcggc gaatattatt tgaccttgga ttttacagcg
    85921 taaatataaa agaaaagttt gattttttgt gtctgatttt gtatgataag gagtagactg
    85981 tgaaaggacg tgtcctgatt ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta
    86041 tcaatgatcg aagtagcaca cgcgatctta gaacaacgtg gcgatgtcat ggatttttct
    86101 gatttagcta accagatcca aaactatctt gggaaatcag acagtgaaat tcgtgattca
    86161 ctcgcacaat tttatactga cttgaatatt gacggcagct ttatctcatt aggggataat
    86221 cgttggggct tgcgttcatg gtatgctatc gattcgatcg atgaagaagt aaaccatgga
    86281 atggacgaag aggatgaaga tactccacgt cgccgtaaac gcaagaaagt caacgccttt
    86341 atcaatgatg atgaagacgc gatcgattac aatgacgatg acccagaaga tacagatctg
    86401 acagaagaag atgacgatga tctgtttgat gacgatgatg atgaagatga agaaatcgct
    86461 gcttataatt ctgacttgca agaaatcgga gcagatgacc cgacagatga tgaagaagac
    86521 ttgccaggaa atatcgaaga agatttaagt atcatagacg aagacgatga tgatgaagat
    86581 tatgaagagg aagaattttc tgactctgaa aaagaataaa cattattttc ttacgagagg
    86641 ttgtgacaga gacgatcagc ctatcagaat aaggaggtgt gacacgattt
//
LOCUS       DACDTO010000003        81290 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDTO010000003 DACDTO010000000
VERSION     DACDTO010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 81290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.003"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..534
                     /locus_tag="IWK70_000175"
     CDS             <1..534
                     /locus_tag="IWK70_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612754.1"
                     /translation="TYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAM
                     IWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVI
                     LMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQF
                     GLTVLGICTFIITVINR"
     gene            complement(774..857)
                     /locus_tag="IWK70_000176"
     tRNA            complement(774..857)
                     /locus_tag="IWK70_000176"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(821..823),aa:Leu,seq:gag)
     gene            1240..2457
                     /locus_tag="IWK70_000177"
     CDS             1240..2457
                     /locus_tag="IWK70_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9612755.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            2641..3477
                     /locus_tag="IWK70_000178"
     CDS             2641..3477
                     /locus_tag="IWK70_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9612756.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            3553..4701
                     /gene="nagA"
                     /locus_tag="IWK70_000179"
     CDS             3553..4701
                     /gene="nagA"
                     /locus_tag="IWK70_000179"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9612757.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            4921..5979
                     /locus_tag="IWK70_000180"
     CDS             4921..5979
                     /locus_tag="IWK70_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9612758.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            5998..7017
                     /locus_tag="IWK70_000181"
     CDS             5998..7017
                     /locus_tag="IWK70_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9612759.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(7119..7835)
                     /locus_tag="IWK70_000182"
     CDS             complement(7119..7835)
                     /locus_tag="IWK70_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9612760.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(7804..8589)
                     /locus_tag="IWK70_000183"
     CDS             complement(7804..8589)
                     /locus_tag="IWK70_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9612761.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(8580..9329)
                     /locus_tag="IWK70_000184"
     CDS             complement(8580..9329)
                     /locus_tag="IWK70_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9612762.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9627..10151
                     /locus_tag="IWK70_000185"
     CDS             9627..10151
                     /locus_tag="IWK70_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612763.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            10167..11666
                     /locus_tag="IWK70_000186"
     CDS             10167..11666
                     /locus_tag="IWK70_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9612764.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(11754..12356)
                     /locus_tag="IWK70_000187"
     CDS             complement(11754..12356)
                     /locus_tag="IWK70_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9612765.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            12460..13416
                     /locus_tag="IWK70_000188"
     CDS             12460..13416
                     /locus_tag="IWK70_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9612766.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(13721..14677)
                     /locus_tag="IWK70_000189"
     CDS             complement(13721..14677)
                     /locus_tag="IWK70_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9612767.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            14851..15411
                     /locus_tag="IWK70_000190"
     CDS             14851..15411
                     /locus_tag="IWK70_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612768.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            15404..16228
                     /locus_tag="IWK70_000191"
     CDS             15404..16228
                     /locus_tag="IWK70_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9612769.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            16238..16855
                     /locus_tag="IWK70_000192"
     CDS             16238..16855
                     /locus_tag="IWK70_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9612770.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            16873..17556
                     /locus_tag="IWK70_000193"
     CDS             16873..17556
                     /locus_tag="IWK70_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9612771.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            18239..19474
                     /locus_tag="IWK70_000194"
                     /pseudo
     CDS             18239..19474
                     /locus_tag="IWK70_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            19482..20369
                     /locus_tag="IWK70_000195"
     CDS             19482..20369
                     /locus_tag="IWK70_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9612772.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            20386..21222
                     /locus_tag="IWK70_000196"
     CDS             20386..21222
                     /locus_tag="IWK70_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9612773.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            21225..21848
                     /locus_tag="IWK70_000197"
     CDS             21225..21848
                     /locus_tag="IWK70_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612774.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            21845..23137
                     /locus_tag="IWK70_000198"
     CDS             21845..23137
                     /locus_tag="IWK70_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9612775.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      23496..23593
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            23709..24290
                     /locus_tag="IWK70_000199"
     CDS             23709..24290
                     /locus_tag="IWK70_000199"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9612776.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            24293..25600
                     /locus_tag="IWK70_000200"
     CDS             24293..25600
                     /locus_tag="IWK70_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9612777.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            25893..26381
                     /gene="purE"
                     /locus_tag="IWK70_000201"
     CDS             25893..26381
                     /gene="purE"
                     /locus_tag="IWK70_000201"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9612778.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            26365..27498
                     /gene="purK"
                     /locus_tag="IWK70_000202"
     CDS             26365..27498
                     /gene="purK"
                     /locus_tag="IWK70_000202"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9612779.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            27511..28806
                     /gene="purB"
                     /locus_tag="IWK70_000203"
     CDS             27511..28806
                     /gene="purB"
                     /locus_tag="IWK70_000203"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9612780.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(28986..29363)
                     /locus_tag="IWK70_000204"
     CDS             complement(28986..29363)
                     /locus_tag="IWK70_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612781.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            29619..30347
                     /locus_tag="IWK70_000205"
     CDS             29619..30347
                     /locus_tag="IWK70_000205"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9612782.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            30347..30601
                     /gene="purS"
                     /locus_tag="IWK70_000206"
     CDS             30347..30601
                     /gene="purS"
                     /locus_tag="IWK70_000206"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9612783.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            30603..31274
                     /gene="purQ"
                     /locus_tag="IWK70_000207"
     CDS             30603..31274
                     /gene="purQ"
                     /locus_tag="IWK70_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9612784.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            31275..33497
                     /gene="purL"
                     /locus_tag="IWK70_000208"
     CDS             31275..33497
                     /gene="purL"
                     /locus_tag="IWK70_000208"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9612785.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            33482..34921
                     /locus_tag="IWK70_000209"
     CDS             33482..34921
                     /locus_tag="IWK70_000209"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9612786.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            34953..35996
                     /locus_tag="IWK70_000210"
     CDS             34953..35996
                     /locus_tag="IWK70_000210"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9612787.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            35993..36571
                     /gene="purN"
                     /locus_tag="IWK70_000211"
     CDS             35993..36571
                     /gene="purN"
                     /locus_tag="IWK70_000211"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9612788.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            36568..38103
                     /gene="purH"
                     /locus_tag="IWK70_000212"
     CDS             36568..38103
                     /gene="purH"
                     /locus_tag="IWK70_000212"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9612789.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            38119..39393
                     /gene="purD"
                     /locus_tag="IWK70_000213"
     CDS             38119..39393
                     /gene="purD"
                     /locus_tag="IWK70_000213"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9612790.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            39515..40429
                     /locus_tag="IWK70_000214"
     CDS             39515..40429
                     /locus_tag="IWK70_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9612791.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(40562..42127)
                     /locus_tag="IWK70_000215"
     CDS             complement(40562..42127)
                     /locus_tag="IWK70_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9612792.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(42120..43571)
                     /locus_tag="IWK70_000216"
     CDS             complement(42120..43571)
                     /locus_tag="IWK70_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9612793.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(43585..44601)
                     /locus_tag="IWK70_000217"
     CDS             complement(43585..44601)
                     /locus_tag="IWK70_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9612794.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            44821..45909
                     /locus_tag="IWK70_000218"
     CDS             44821..45909
                     /locus_tag="IWK70_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9612795.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            45963..47489
                     /locus_tag="IWK70_000219"
     CDS             45963..47489
                     /locus_tag="IWK70_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9612796.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            47491..48696
                     /locus_tag="IWK70_000220"
     CDS             47491..48696
                     /locus_tag="IWK70_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9612797.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            48953..49930
                     /gene="guaC"
                     /locus_tag="IWK70_000221"
     CDS             48953..49930
                     /gene="guaC"
                     /locus_tag="IWK70_000221"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9612798.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            50194..51618
                     /locus_tag="IWK70_000222"
     CDS             50194..51618
                     /locus_tag="IWK70_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9612799.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            52079..53188
                     /gene="pdhA"
                     /locus_tag="IWK70_000223"
     CDS             52079..53188
                     /gene="pdhA"
                     /locus_tag="IWK70_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9612800.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            53191..54168
                     /locus_tag="IWK70_000224"
     CDS             53191..54168
                     /locus_tag="IWK70_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9612801.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            54193..55836
                     /locus_tag="IWK70_000225"
     CDS             54193..55836
                     /locus_tag="IWK70_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9612802.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            55844..57250
                     /gene="lpdA"
                     /locus_tag="IWK70_000226"
     CDS             55844..57250
                     /gene="lpdA"
                     /locus_tag="IWK70_000226"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9612803.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            57412..57687
                     /locus_tag="IWK70_000227"
     CDS             57412..57687
                     /locus_tag="IWK70_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9612804.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            57693..58460
                     /locus_tag="IWK70_000228"
     CDS             57693..58460
                     /locus_tag="IWK70_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9612805.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            58628..60457
                     /gene="typA"
                     /locus_tag="IWK70_000229"
     CDS             58628..60457
                     /gene="typA"
                     /locus_tag="IWK70_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9612806.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            60718..62490
                     /gene="recQ"
                     /locus_tag="IWK70_000230"
     CDS             60718..62490
                     /gene="recQ"
                     /locus_tag="IWK70_000230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9612807.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            62604..62906
                     /locus_tag="IWK70_000231"
     CDS             62604..62906
                     /locus_tag="IWK70_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9612808.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            62909..64072
                     /locus_tag="IWK70_000232"
     CDS             62909..64072
                     /locus_tag="IWK70_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9612809.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            64098..67526
                     /locus_tag="IWK70_000233"
     CDS             64098..67526
                     /locus_tag="IWK70_000233"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9612810.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            67547..68671
                     /locus_tag="IWK70_000234"
     CDS             67547..68671
                     /locus_tag="IWK70_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612811.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            68661..69098
                     /locus_tag="IWK70_000235"
     CDS             68661..69098
                     /locus_tag="IWK70_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9612812.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            69082..69420
                     /locus_tag="IWK70_000236"
     CDS             69082..69420
                     /locus_tag="IWK70_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9612813.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            69539..70099
                     /gene="rsmD"
                     /locus_tag="IWK70_000237"
     CDS             69539..70099
                     /gene="rsmD"
                     /locus_tag="IWK70_000237"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9612814.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            70096..70587
                     /gene="coaD"
                     /locus_tag="IWK70_000238"
     CDS             70096..70587
                     /gene="coaD"
                     /locus_tag="IWK70_000238"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9612815.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            70577..71620
                     /locus_tag="IWK70_000239"
     CDS             70577..71620
                     /locus_tag="IWK70_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9612816.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            71779..72507
                     /locus_tag="IWK70_000240"
     CDS             71779..72507
                     /locus_tag="IWK70_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9612817.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            72623..72868
                     /locus_tag="IWK70_000241"
     CDS             72623..72868
                     /locus_tag="IWK70_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9612818.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            72865..73083
                     /locus_tag="IWK70_000242"
     CDS             72865..73083
                     /locus_tag="IWK70_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9612819.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            73219..73758
                     /locus_tag="IWK70_000243"
     CDS             73219..73758
                     /locus_tag="IWK70_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9612820.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            73751..76024
                     /locus_tag="IWK70_000244"
     CDS             73751..76024
                     /locus_tag="IWK70_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9612821.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            76062..77108
                     /gene="holA"
                     /locus_tag="IWK70_000245"
     CDS             76062..77108
                     /gene="holA"
                     /locus_tag="IWK70_000245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9612822.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(77197..77451)
                     /locus_tag="IWK70_000246"
     CDS             complement(77197..77451)
                     /locus_tag="IWK70_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9612823.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(77851..78120)
                     /locus_tag="IWK70_000247"
     CDS             complement(77851..78120)
                     /locus_tag="IWK70_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612824.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(78590..79045)
                     /locus_tag="IWK70_000248"
     CDS             complement(78590..79045)
                     /locus_tag="IWK70_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9612825.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(79183..81111)
                     /locus_tag="IWK70_000249"
     CDS             complement(79183..81111)
                     /locus_tag="IWK70_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9612826.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT        26367 a        13977 c        17603 g        23343 t
ORIGIN      
        1 acatatagga aatgtcaaaa ctattatgtg cgtatattga ctgcattacc gtttttctta
       61 aacctgatta tttattttgg taatacaatg ggtcaaagct ttacttacgt caatggaaat
      121 aaaagggcaa tgatttggag tagtagtaac ctgaataatc tctttacaga gttaggaacg
      181 aaactgtcac ttttttatcc aggtacttgg atagctactt tagtagtctt ggcattatta
      241 ctatgtttaa ttgttggaat ttatttaagt tttgacaata agaaaacttc tattttttta
      301 gttattttga tgataatggg attttgttca aggttaatta tgggattttc tcccacagta
      361 tgggcttccg gaatgcggac gtactatata ctttatgttg ttatcgccat cttagtacta
      421 atggcagtta aagagctaat gaaaagcatg agtgtacaaa aaaatgaatt tatgcaattt
      481 ggattaaccg tgcttggtat ttgtaccttt ataataacgg tgataaacag ataatataca
      541 aaaaatccct cgctttagta ttaggtgagg gattttactc gtttttcgat taattgtttt
      601 aacacttgca agtttctacg agtagcttgt ttccgacctt catatactca cctcaggctg
      661 tttacatgat tgtacgttct ttttgtaaag gatacagtga tatgtatata atggcataaa
      721 gaaacgttca aacaaaaaaa ctcgtgaagc aaaattaact tcacgagttg tagtgcagat
      781 gatgggactc gaacccacac gagctaacgc tcacatgacc ctcaatcatg cttgtctgcc
      841 aattccaaca catctgcgta ataataacac actaatgata gcgaggtttt acatagttgt
      901 caagctcaaa aatgggagga cttattaaga tggagaaata tgacggagaa ttttcaggac
      961 tgggaatgat cttaggtgta ttgattggac ttgcattcgg acgttttttg cttggtttta
     1021 tgctaggaat catttgtgga attgcaatgg attgggcagc gaatctatgg aatgactatc
     1081 atgacaatta agttctcaac gcttgtttgt gtttttttaa tacttacgtt tttcctaaat
     1141 aagtggcaaa attttctagg atggagtatg atattgaaat agtaaataac aaaaaatttg
     1201 gaaagttcta actttttgaa atgagggatt ggctaagtga tgagagaatt tgaaaaatcc
     1261 aacaaattag aaggtgtcag ttacgatgtt cgcggtcccg ttctagaaga agcggagcgg
     1321 atgcaagaag agggcattag tattttaaaa ttgaacacgg gaaatcccgc accttttgga
     1381 tttgaagcac caaacgaagt cattcgtgat ttgatcatga atgcccgtga ctcagaaggc
     1441 tactcagatt ccaaggggat cttttctgct agaaaagcaa ttgagcaata ttgccaactg
     1501 aaacattttc cgaatgtgac gatcaatgat atctacaccg gcaacggagt gagcgaattg
     1561 atcacgatgt gtatgcaggg gttgctggac aatggagatg aagtactggt tccaatgccg
     1621 gactatccgt tgtggacggc ttctattgca ttagctggag gcacacctgt tcattatatt
     1681 tgcgatgaag aggcagaatg gtatcctgat attgatgata tcaaatcaaa aataacgagt
     1741 cggacaaaag ctattgttat cattaatcct aataatccta ctggggcact ttatccaaaa
     1801 gaattattag aagaaatcgt tgaagtagct cgacaaaata acctgattat ttattccgac
     1861 gaaatctatg atcgcttagt gatggatggt ctagaacata ttccgattgc tacattagcg
     1921 cctgatttgt tcgttgttac gctgaacggt ctatccaaat ctcatcgtgt agcaggattt
     1981 cgagtaggct ggatggtcct aagtggagat aagtcacacg tcaaaggcta tatagaaggg
     2041 ctgaatatgt tgtcctccat gcgtttgtgt tccaatgtat tgtctcagca aattatccaa
     2101 accgcgctag gcggttatca aagtgtcgat gagctgctcc tgccaggcgg aaggatctat
     2161 gaacaaagag aatatatcta taatgcaatc aatgatattc ctggtctttc agccgtaaaa
     2221 ccaaaagctg ctttttatat attcccaaaa attgatacaa agcgattcaa tattttggat
     2281 gatgaaaaat tcgttttgga ctttttacat gaacatcata ttctacttgt acatggtggt
     2341 ggatttaatt ggaaccagcc agatcatttt cgaatcgttt atcttcctaa aatggatgat
     2401 ttgaaacaaa cagctaagaa aatgagagaa ttcctagcaa cctatcgcca aaaatgattt
     2461 taaaaaccaa atttttcagt aggagggcca cagacaaaca cactgaggct ctctttctag
     2521 tggagttttc ttaaataact aaagtatatt atttagttgc aagtcttttc ttgaagtttg
     2581 cgacttccta aagtaaaata cgaatagaat gcgaatacgc atattttggg gaggatactt
     2641 atggaaacgt taatcgagtc ctttggaacg atcattaaag agattcgaaa agaacaaaaa
     2701 atgacgcaaa aaatgctttc gcatgatatt tgttcacaaa gtgtattaag tcggatcgaa
     2761 aataatgagg aactgccgaa tgtattggtg atgatgcaga tttgtcaacg gctaggggta
     2821 acgattgatc atgtgatgaa tctctctcga aaaactactc ggacaaacga taaattattt
     2881 gaactgttgg atagttgttt ccaaaaacgt gattatcgga agttagaaca agtactaaaa
     2941 tcttctgata tttctgaaaa tctgtatcaa gcaactgact ttcaacgcta ttattattat
     3001 ctcggtgttt gcgaattcac gttgcgtcaa aatatctctc agtcccttta ttatttgaaa
     3061 gaagcattaa gctactctag ccaaaaagaa agaatccatg tttctgacac agaaatacaa
     3121 ctgattagtt gtatcggaaa aatatatggt gtggcaggaa aaacggctga agcacgctac
     3181 tatttgagtc gaagcatcca actgctgcat caatcagctg atgaacgatc aaaaagtgaa
     3241 ttttcgcaaa ttttttataa ttatggaaat ttcttattcc atcaaaatga aatcgacgaa
     3301 gcgttagaac aggtgaatca aggaatctat tgggctcaag agaagaatag ctactattac
     3361 ctcaatgatt tatttgtgct taaaagctta atatacaagc gtaaagaaga accagaaaga
     3421 gcagcattct atgaaggctt agcacaagct gtgaaacaaa tttcaaaaaa tttgtaaatg
     3481 gtgtagacca ttcttgactt cttctctaaa taagagtact ttagaggtag aaaaacaaag
     3541 ggagagaata gaatgagaac ttttatctat gctgacaaat tctttttagc gtcaggtgtc
     3601 aaacatgcag gctatcttga aattaccgat ggactttttg gtgaatacac caaagaacta
     3661 cctgagggag aagtcaacat cattgaccaa agcggcaaat ggattgctcc tggtttggta
     3721 gatacacata ttcacggcta tatgaatcat gatgtaatgg ataatgatgc agaaggcatc
     3781 aaagtgatgt ctgaaggtct gttatcttgt ggagtgactt catttttacc aacaacattg
     3841 acgtcaagca aagaacgttt aaaagatgta gctgagacaa tcggcaaaat gtatcaggaa
     3901 gcaccaggag ctaaaatcaa aggaatctat tttgaaggtc ctttcttcac agaagaacac
     3961 aaaggggcac aaaacccaag ttatttcggg gatcctgatt tggatacttt ccatgaatgg
     4021 caagaagctt caggtggttt aatcaaaaaa attgctttag caccggaacg tcaaggtgta
     4081 gaagagtttg tcaaacaagt gactgaagaa ggagtagttg tttctctagg acacagtaat
     4141 gctacactag aagaagcgca aaaagctgtt gaagcaggcg caagtgtatt tgtccatgct
     4201 ttcaatggta tgcgtggatt gaatcatcgc gaaccaggta tggtgggtgc gttattatct
     4261 ttagaacatg tcttttctga attgatttgt gatggccatc atgtacatcc aaatgctgcg
     4321 ggaatcctaa tggaaaaagc aggacatgat catgtcgcat taattacaga ctgtatgatg
     4381 gccggtggga tgcctgatgg aaattacaat ttaggcgaat ttccagtagt agtaaaagaa
     4441 ggaacagcac gtctagaatc tggcaactta gctggaagta tattgaaatt gaaagaagca
     4501 attaaaaatg ttgtagactg gggattagca acaccagaac aagcagtcat gatggctagt
     4561 ctgattccag caatcagctg taaaatcgat gatacatgtg gaaaaatcgc tcaaggacgc
     4621 gatgcagact ttatcgtatt gaaccctgat atgacattgg aagcaactta tctagatggc
     4681 gtggaacgtt tccacgcata atataattta agttaacctt aaaactaaac ttcttaagtt
     4741 tggttttaag gtttttttgt gttctagtaa aaatcacttt acataagttt caaaagtttc
     4801 tttaatttcc tattgttcaa taagtaacaa tgtaaagata aaattaaaaa tgttaatacg
     4861 tattagcaaa ataaaagctt tttttattgt gaataaatga acataattag gaggagaaag
     4921 atgaacttat ttgacatttt tcgatcgact ttgacagata tgtcgattgt cagtgcaatc
     4981 acttcaacag tttttattat attattaggg tttgtttgta gaaaaaaggg gattttttct
     5041 gaatctgttg gaaaaattct atcgaaagtc gtcttaacag tggctttgcc agcactggca
     5101 tttcgagctt tcatgcaaga tatcaataca gaattactga tgcaaggaat caatgtcttg
     5161 atttggggat tagttattta tattattctt attctagttt caaaaccttt tttcttaagc
     5221 tataaagggg ataagcaaac aacgttgcgt gtcctgacga tctttggttc tacgaccttt
     5281 ttcggaacgc ctatcgtcag tgcgatttat ggtcctgtcg gcgtgatgta ttcctcaatt
     5341 ttcaatattg gctaccgaat ctttttgtat tcttatggat acataaaaat gagtggcttg
     5401 aagatggaag ctaaaaatat caaagcgatg ttcgtcaatc caatcattat cgctactttt
     5461 gccggccttt tcatttggct gttccaaggg tatctgccac aagtgtcagt tacaggtgca
     5521 gaaggagaga taagccaagt agcgtttttg cgaattgacc agactgcacc gtggctattt
     5581 cagccgatgg attatttagc tggtttaagt tcaccattgg cgtggctagc aatcggcgca
     5641 acattaggtg aagtaagctt caaaaaagct gcatctgaaa aaacttcatg gtattacagt
     5701 ctagtcaaag tggtatttgt acctgcattg aatatcgtga ttctagcagt tttgacggca
     5761 acgaatattc taccagtcaa ttttgaagca ctggcaacaa tcgttatcat gatggctaca
     5821 ccagctgcaa cagtggcagc agcctatgct atcagttttg atagggaggc acttttagcc
     5881 tccaatgcat ccttactgtc aactattttt gctgtggtac tgacacctgt atggatcgtt
     5941 gtattacaaa tgattcatgg cataggttta ttttcataag aaatggagga agaaagtatg
     6001 agaatcgcat gttacggtgt tcgtccgaac gagatagatt tttttgaaaa attgaatatt
     6061 taccattatg atctcagtct ttatgaagaa ctgttgacac atgacaatat cgtgtcagca
     6121 aaggaccatg atgctgtatt gttgagggga aattgtatag cagatgagac caatcttgca
     6181 aaaatgcagg aatatgggat tcgttatgtt tttacacgga ctgtgggagt caatcatatc
     6241 gatctacaag cggcagctga ttttggtatg acagtagccc gtgtaccatc gtattcgcca
     6301 aatgcgatag cagagttatc tttgactttt gccatgatgc ttttgagaaa tactgcgtat
     6361 acgacgatcc gaacatcttt caaagatttt cgagtggatg agcaaatgtt tagtcgtgaa
     6421 atcagaaatt gcacagtagg gatcatagga acaggcagga tagggttgac ggaagctgtt
     6481 ttgtttaagg gactaggagc caaagtgatc ggctacgata ttcatcagac agaagctgct
     6541 aaaaaagtat tgacgttcca ctcgcttgaa gagttattag ccaaaagcga tattgtcagt
     6601 attcatgtac ctcatatccc aggtgaaaac gatcaaatga tcaatgaagg atttttagct
     6661 caaatgaaaa aaggtagcat cttgattaat acagcccgtg gagagcttca agataatgaa
     6721 gctattttaa gagctttgaa aagcaatcat ttagctggtt ttgcaaccga tgtttttgca
     6781 aatgaaacag aggttttttt ccgatccttc aagccatggg agacgattcc agaccctgct
     6841 atccagcaat tagtagaaat gtaccctaga gttcttgtaa ccccgcatgt aggttcaaat
     6901 acagatgagg cattatcaaa tatgatttcg acaagtttcg agaactttcg tgaaataata
     6961 gaaactggaa aaacgaaaaa tgaggtatct cttcctaaag cccgtcaatt aagataagaa
     7021 aacatggata ttcgtcaata aaatgtttaa aaatagtcca agtaaacagt tgttacttct
     7081 gtatacttgg actattttgt atgggataat tcgatgactc aacagtaatc tgtgagcttt
     7141 ttccgcccat tctttgtcaa ttcataacag gttagcggct ggccgatttc caaatatttg
     7201 attctttcgt tcaatatatc ttgttctact aaaaaattca aatatttctt gacggatata
     7261 cgtgacagtg agcttttttg agccagttct tttgttgaaa aaatagtatc ttcttttaga
     7321 ataaatgcac ttattttttt taaagtcagt tttgacagac ctttaggcaa atcgatgaat
     7381 gatggtattt cgtgaatggg agtggtgtgg ttgaatactt gatcgatcaa tttctgatcg
     7441 gctttttcag tcgtttggat tgtctgagag aaagcaagat acttttcgat cgccagctga
     7501 aaacgttgca aagtgaacgg tttgatcaaa taatctaagg caccgtagcg gatcacattc
     7561 tttatggttt cgatatcatc tgcagcagtg atcaaaatca ctgggatatc atttccttgc
     7621 tgtctaagcc aagataaaaa atggatacca ctttcgccag gcatgtatac atcaagcagc
     7681 agcagatctg cgtttttttg tctgaaatat ttttttcctt cttctacatt tctgaaactt
     7741 tcgattacgt tcatattagg gatgctttcc acaaaagtac gattcaattc tgcaaccatt
     7801 ggatcatctt cgattatcag gacatttgtc atcattttga tcttcctttt ttggtaattc
     7861 tagatagacg attgtaccgc catttgtttg tttggaaaat tctaaaaatc caccgtggcg
     7921 tttgacgatt gaatggatga gattcaatcc aaacccacga ttgattccct tcgtcgaaaa
     7981 acctagcgta aatatttttt ctgagatttc ttctgatatc ccgacaccgc catcgcagac
     8041 ggtcacgatg aatacttcag cttctgtgtc atagtttaaa tgaatccgga cctgtccgtt
     8101 tctttcatgt ttttgtagca cggcttcttt tgcattatca agtaagttcc ctaacacttg
     8161 caaaagtgaa tggatggagt catccatctc cattttaggg ataaaagaat cgctttctaa
     8221 cagcatttca acagattgtt ctttggcttc gtttattttg cctaataaaa aaccggcaat
     8281 tgcaggtact ttgatcagac ttgagacttg gccaatctct tttttataat tttgatttag
     8341 ctgctggata aaatgggtga cttcttcata cttcttcaaa tcaatcaaac cagaaatgac
     8401 atgaagttta ttcatgaatt catgggtttg agctctcatg gagtttatat attgtttcgt
     8461 ccccccaagc tcctccatta atttcattac ttctgatcga tttcgtaaag ttgcgactgc
     8521 tcctaaaaaa cggtcattgc taaaaagagg tgtaacgcta gaaatagttt ctatcccatt
     8581 taaatacatg gattgagtcc gaacggtcgt ttgatgctca atcgtctctt taaacaaaac
     8641 gtcatataga ttatcactga tttttttcct acttcgaaat ccaatagctg ctctgtttgg
     8701 tagaaaatgt tttttgcagg tgtattgatc aagaggatag ttttatcaga agagatggct
     8761 aagattcctt catctacttc attttcgata atcgtttttt cacttaactg ttctgctatt
     8821 tcttgtggtt caagtccaag cagtactttt ttcatttttt gggcaagaaa aacggcgcct
     8881 atgatgccta cgctaagtcc aataatcaat gaaaaaacaa tcattttttg tccatctttg
     8941 acttgattgt caatcgtgtt gtttgttaag ccaacacaaa ctgcaccaat aatctcttga
     9001 tcataataaa tagggacgaa atagcggaag ccgaacccta acattccttc tttttcagag
     9061 aaaaattctt gtcctccaat cacttttttc acatcttcat gacttgagaa aggaagaccg
     9121 atgtaacttg ggtctgcgtg agaatatcga atgccttcag ggtccatgac aacgataaaa
     9181 tctaaattcg atgcaatcat gatttcattt gttattcctt gaatcgcatg tgtcgggtca
     9241 ccagtctgca gctttttttg gatgagttta tcattggcaa cgattttagc aatgctagag
     9301 attttatctg acacactaga tcggttcatc gaaagaataa aattcctgat caatatggca
     9361 gaaacaatca aagaaacgaa tgtggagaaa caaacgacaa ggatgatcgt gttctggagc
     9421 tttaatggtt ttttcttaaa aaaaggaaac ataggctacc tcgaaagaat ttattgtgaa
     9481 aattttaaca tgaaaacaaa aatttaacaa attgtcaacg ataaacacaa ccttcctata
     9541 aaaaacacca ctgagtcggt aaaagaagct atacagaatt ttcaaacatt ggtatgatag
     9601 acgtagaaat ggaaggaggc gtgtatatga tagggggaat cgcacttgag acgatttatt
     9661 ggtatacatt gctgatttgt gcaggattgg cagtcctatt gattattttt ggagatgtat
     9721 tcgattttga tggcccgctt gatccgatgc ttattatccc ttggttaact ttcacaagct
     9781 tatttggttt tctaggtgag cgtttaacag atcaatcatc tttattttta tttattgcta
     9841 gtggaggcat atcgatagtg ttggtatttt tgcttaattt ctatatattg atgccgatga
     9901 aacatgccga atcaacgatg tcgtcttctg aaaaaactct ggaaggacaa gtagctgttg
     9961 tgatcacacc gatccctgta cgaggtatgg gagaaattca gttgaaaagt gttaccggtt
    10021 ccattagtcg tccagcttgt ttttacagcc cccaagaaca gacagctgag cggggaacga
    10081 aagtattgat cattgagatc aaggaaagaa tctgttatgt catgccttat gaaagcatga
    10141 taaaagctta aaggagcgtg tgttaaatgg aaataccagt tgtttttatc gcgatcgcaa
    10201 tcgcagcttt gattttactg atgttgctga tcgtctttgt agcgaagtac cagacagcaa
    10261 aaccggatga agcgttgatc atcagtggaa gttatttagg aagcaaaaat gtgcacattg
    10321 atgaaagcaa taacaaaatc aaaattgtgc gtggaggtgg cgcattcgtc ttgccagttt
    10381 ttcaacgttc gaatcggatc agtctattat cgagtaaatt ggacgtctcc acccctgaag
    10441 tgtataccga acaaggcgta ccggtgatgt gtgatggtac ttcgatcatc aaaatcggtt
    10501 catctgtaga agagattgct acagcagcag aacagttttt aggcaagaca agagaagaac
    10561 tggaaaatga agcaagagaa gtattagaag gacacttgcg ctcgattctt ggttctatga
    10621 ctgtagaaga aatctatcaa aatcgcgata agttcagtca aagcgtacaa gaagtcgctt
    10681 ctgtagacct tgcaaaaatg gggttgatca tcgtttcttt cactatcaaa gaggtcagag
    10741 acaaaaacgg ttatcttgac tctttaggga aaccaagaat cgcccaagtc aaaagagatg
    10801 cagatatagc agaagcggaa gctctaaaag aaacgcgtat caaaaaagct caagcagaaa
    10861 aagaatcaca agcagctgaa ttacagcgac aaacagagat cgcagaatca ttaaaagaaa
    10921 aagaactgaa attagcaacc tacaaacaag aacaagatgt tgctaaagca aaagcagacc
    10981 aagcctacaa tttagaaagt gcccgagcac aacaacaagt aatcgaacag gaaatgcaga
    11041 ttaaaatcgt agaacgccaa aaacaaatcg aactggaaga aaaagaaatc acacgtcgtg
    11101 aaaagcaata tgattcggaa gtgaagaaaa aagcagatgc cgatcggtac gctaaagaac
    11161 aagaagcaca agctcaaaaa gtcaaagaag tgactgaagc cgaagcagaa cgattccgtg
    11221 tggaagcact ggcagaagcg gaagccaata agacacgttt agctggacaa gcagaagccg
    11281 aagcagcact agcaaaagga aaagcagaag ccgaagcaaa acaaaagatc gccaatgcct
    11341 tcaaggaata tggcgaagca gctgtcttga gtatggttat cgatatgcta ccgcaattga
    11401 tgcgcgaagc agcacagccg ctaggaaata tcgaaaaaat ctctgttgtc gatacaggaa
    11461 gcagttcagg agaaaccagt ggagcgaacc gagtgacaaa ttatgccaca aatttgcttt
    11521 cttctactca agaaacacta aaagcgacaa caggacttga cttgaaggaa atcgtagaga
    11581 atttgtcgac acgtgggact actagccaag taaatatgta tgaagcgcca aaagaggcag
    11641 aacaaaactc gatgaaggaa gaataaagta gtacacttca ccgttcaatc aaaaatggag
    11701 gaagactttt taaagttctt ctctccattt ttttatttta ttgcaaaaga aaattagtcc
    11761 tcgctatttt tcttttgttt ttggatctta gcttctatct cttgtaaatt tgttcttagc
    11821 aatcgttctt tcttttggag ttcgaccaaa tcttctgata gttcttcctt cttttctgtt
    11881 tcaggaggac gaagaaagaa attgataccg attgcaatga atgtccctat aaaagtatct
    11941 agtacacgtt ggatagcata caaagcggat tcgccttgag ggatgctcaa cgcaatcagc
    12001 aacaacgtag ctattgcgga aatgataccg gaattattgt taataccatc tgacacgacg
    12061 atcacaataa taactagaat aggaagcaag atcaattcta ccaaaaaatc attgtgaaaa
    12121 tagcttttga caaaaaaata aatgatagct aatgtaccac ctaatgtatt gccgatgatc
    12181 ctagactttc caaaagaaac ggttgtagtc aagtcctgac gcaaagaaaa aacagcagat
    12241 aatgcagcaa tcatcgggat tcctcgttga gtcaaatgaa aaaacaagat acataaaaaa
    12301 acagctaagg cacttttgat cgtacgcata cctagtcgaa aacggccaaa ttgcatagag
    12361 attctccttt ttggtctttt tactatcata cctaaacttt tgtctgatag ctagtttttc
    12421 attattcaag tataattaaa ataaaaaagg gggaagtaaa tgcgtatagg aatgcgaaca
    12481 gtcaaaacag tgatcagtgt cttcttttcg atggtcacag cgggtagctt atcgctgttg
    12541 tattggccag ctgcagggat cattgcatta ttaagtgtag gaaataccaa gcgctctaca
    12601 ttgcgtacgg gcatgaatcg aatcgcagcc tttatattag ctacggcaac agcactgttc
    12661 agtttttttg ctgttgggta tactattttt ggcttcagtc tttttttatt cctgttcatc
    12721 ccgcttgcct ctcgcttcaa attaaccgaa gggatagtag taaattcggt tttggtcact
    12781 cattatttag tggaggaaaa catgtcttgg cagctgatcg gcaatgaagc gggattaatg
    12841 gcgattggat tagtatttgc tttgcttgca aatgtttaca tgccagatcg tacgaagcag
    12901 ctgaaggaaa accaaataca gatcgaaaaa gaatttcgaa atattttgag acaaatggca
    12961 gaatttcttt tatcagaaaa taaagaagat gtgcagatca aatgcgaaca tttactgaca
    13021 ttcattcgcg aaagccaaga agatgccaga gaacatcaag aaaactactg gctcagacag
    13081 ccgctatact acgaaactta cttctctatg agaagagcac aggccaatgt gattaaagac
    13141 atgctggaaa atctagagag aatccagcaa ccagcttatt atggaaaaca tatctatggt
    13201 ttattgattt atactgcaga aacattttca gagtcaaatg atggcagaca aattttaaca
    13261 cgtattgaag aagtttatgt attgtatcgg caaatgcctc tgccgactag tcgccctgaa
    13321 ttcgaggatc gagcagaatt gttccagttt ttgcagtctt ttaaaagttt tattgaaatc
    13381 aaggcagaat tctcccaaca aaaaatccaa aaatagaggt tgtgaattca gctaatattc
    13441 aggtcaaaaa gctgatttca caacctcttc tttaaacggt gttctcctgt caactcctcg
    13501 gtcacaagtc acaatattca acaaacatga gaagctgttt tttgaaattg ctccttgtga
    13561 ctcggagctg aacggtaggc tcacaacctc tttcttaaac ggtgttcaaa agctttcctc
    13621 tgcggtaata agtccagcca aacgaaaaat aggaggagct atttttgttt ggctgttctt
    13681 attacttgat gcagaacagc tttttcacaa ccttttaaaa ttacatattt ttccatagaa
    13741 cttgcatttg atccaagatt tcgatgacat cttttgtcaa gaagagagag cgattgggca
    13801 agtcaccttc aatcatgtgt accatattct ttatttcgta attcattgct tcagatgtat
    13861 tcccgctttc gatgaattcc tttgtaccat ctgtaaataa gatctccgca cgatcagcac
    13921 gcgggtaatc agcaatcgtc acataaccgt tctcaaaggc aacgataccg acttttggca
    13981 tttttgcctg aaaagtcaag ctgacagcag caagttcatt ttctttgtta cgcaagatag
    14041 tgacggattg ttcgtccacg cctgtttcaa aaggaaccat tgtagaggca actacttcag
    14101 gttgagagct caaaaagaaa cgagcgaaag atacagcata cgtaccgata tccaacagag
    14161 ctcctccagc taattctgga ttgaagaacc ggtttttagg gtctggttct ttgtaactgc
    14221 cgaaaggtgc ttggatcatt tttaaagccc ccagttttcc tgtatccatt attgagcgta
    14281 actgctgata aagtggcatg ttgaagattg tcatcgcttc tgctaagatc aaattctttt
    14341 cttctgctaa gcgcatagcg tcagccagtt cttttttatt catcgtgatg gctttttcac
    14401 atagaacgtg tttgcctgct tccaatgctg ccaagatatg gtcgatatgc tgtctatttg
    14461 gtacagcaat gtacacgatg tcgatttcag catcgtctaa catttcttga tagcttccgt
    14521 aagcttttgg aatattgtaa cggtgagcga agttttccgc tttttctgaa gttctggaag
    14581 caactgctgt tagttcgctc gtctcttgat tgaagctttc ggcaaactca tgtgcgatgg
    14641 taccaagacc aacgattccc cattgatatt ttttcatctg aaaacgctcc ttcatagttg
    14701 gattgagttt cttgtctgtc tattattata acaaaaatca cgaagaataa agaggagagg
    14761 acttgaaaca ttaaaaaaaa tgaaagcagc gatttaagag gtgctttttg atcaaaactt
    14821 aagtagaata gttaaaagga ggggataaac gtgcatcaag tatatattga tcttattatg
    14881 agtgtattat tagaacagtt tgaaactgaa aaaaattttt tagatgaata cttgctgatc
    14941 agtccagaac aatggcaaaa atggaaagat ggcgagaaaa atctgccatc tgaagcgatg
    15001 cagaaagtaa aaaatctttt cactgattat gaatggatgc taacgcaaaa gattttgcgt
    15061 caaacgatta tttttcccga aaaacgaaac gttgccgttg cagagtacaa gcaaatcaaa
    15121 acacgcatcg cgcaaaaatg gcttagcagc aaagcagcaa aagtcgagct gatccctttg
    15181 aaaaataatc agcaggctgg tagatacctt gacttgaaag taagtattca atatgatgaa
    15241 tggggctatg atgatatttt gaattttcgt ctgccagcag atgttcaaaa acagatcgaa
    15301 ggagaaaaaa tcgaacttct cgattgggtg aatgaaaatc tggaagaaac ttacgttcat
    15361 gaggcgaatg gggaacgtga agaacaacga aaggattttt caagtgaata aatttttagg
    15421 atttggagca gttgtgatta tgatcgcagc aatgggctat gttattttta gcgatgatga
    15481 ttcgcatgtt caagccggca atcaagcagt cgaacgaaca agcaccaaaa caactgctag
    15541 taaagaaaag aacagtcaaa caaccagcag caaggtaaag gacaaagaaa caaaagatcg
    15601 tacgtccaaa caattgccag atgttaaaac agacgattgg aagctagttt tggttggtcc
    15661 agatcacaaa ttagaaaaag aaattgatga gtctactcaa ttggcatcat taagtaacgg
    15721 atacttgatc gacaaacgca ttgctccaga atatgaagca tttgccaagg ctgctgaagc
    15781 agctggattt ccattagtga tggtctcagc ttaccggtct gtatcatccc aacaacaagt
    15841 tttcgaacaa aatgtccaag atgtgatgag tcgtcaaggt gtatcagaag aggaggcgac
    15901 aaagattact aaagaaacga ttacggagcc aggatacagc gaacaccaca caggtctggc
    15961 agtcgatgtc gtcgatcaaa actggtacaa cagttatcct tctactgtat tggatgcaag
    16021 ttacggagat cagcctggag ctaaatggat cgcagataat gctgcgcagt atggatttat
    16081 cgttcgttat ccaaaaggac acgaagatat aactaagatc acatatgaac cttggcactt
    16141 acgttatgtc ggtaaagaaa acgctgagta tatcaccaaa caccaattga cgctagaaga
    16201 atatctgaat gaattaaatg aaaagtagga attgatcatg gcgaaaaaaa agtggaaaaa
    16261 gcaaaagcgc aagaatatcc gttggccagc agtggttgcc ggtgtagcag tgatactctt
    16321 agcctttgtt ttttccttga agaatctgtc ttctcctttt actgataatc agcaagaaaa
    16381 tgaagcgcaa tcccatcagc aatttattga acggcttgtt ccgcatgccc aagaactaca
    16441 agatggttat ggagtgcttc ctagtatcat tttaggacaa gctatattag aatcaaattg
    16501 gggaaaaagt accttggcta gcaaatacaa taatcttttc gggatcaagg cttatgggga
    16561 tcagaagaaa gtaagcctgg aaacaaagga atttgttaac gaagaatgga taactatcca
    16621 aggagacttc aaagtgtatg attcatggga acagtccatg gatgaccata cacaactgtt
    16681 cgtccaagga gtagattgga atcctgcgct gtatgaaaaa gtgattacgg ccaccaacta
    16741 tcaggaagcc gcacaagcat tgcaagatgc aggttatgcg acagaccccg gttatgcgca
    16801 aaaaatcatc caggtcatcg agacgtatca attgaatcag tatgaccgga gatagcaaag
    16861 gagaagtggc tgatggcaga acaatttacg ccagaaatca tgacggaatt gagaaaagat
    16921 atcgtaagag taccggaaat cattttagaa gcaagcggga tcaagatatt tggaaaattg
    16981 atccgttcga tcatctttac tacggatatt gcgatcatac gtaatacaaa tgcgaatgca
    17041 gtaattgctg tgtatccttt tacacctcat ccagcgatta ctaaaagcat tattgaggca
    17101 gctgacattc cagttttctc cggtgtaggt ggtggtttga cacaaggtgc tcgttcttcc
    17161 tacatgagca tgttcgcaga agcgcaaggt tcgttagggg tcgttttgaa cggtcctacg
    17221 cctttagaga cagtaaaaga agtttgtcga gtagttgata ttccagttat cagtacggta
    17281 acgtcgaaat atacagaaat agatgaaaag ttagcagctg gtatttcgat tatcaatatc
    17341 agtgcaggta aagaaactgc agaagcagta cgccattttc gtaaaaaata tcctgaactt
    17401 cctatcattg cgacaggtgg tccaacggaa gaaagtattg ctgagaccat tcaagcagga
    17461 gcaaatgcca tcacatatac gccaccaagt agcggagagc tttttagtcg taaaatggaa
    17521 aaatatcggg aaactgagaa aaagaatcat gaataacagg tgagaatcaa aagacgagga
    17581 agagcagata gtggctcttc ctcgtctttt gtactccagt actatttgtt cagaattcaa
    17641 aaaaaagaga aatttttttg tttttttacc tgtaatgtac ataagaagag ttaaaacctt
    17701 ttgttattat tagcattcta acaaaaaaat gtcttgattt attgaagcgc tttctataga
    17761 atgaaaaacg aataaaaact aaaaaaggca cccgacaatt tagtagcagc taaattgtcg
    17821 gggcttgaaa agtcagcggg aactgaccaa tcaagttgcc atagtcaatg cttgtgcatc
    17881 gacgtgtata ttgtacctaa tttcatacag aattactagt tatttttctg ttttttaggg
    17941 aagagcgcac acgaagaagc atgcttattt agctatgtca aggatattgg gcagttgtct
    18001 gatatccttt tttctttgcg taattatcct cccatcattg aagcaagaag tgtttttagc
    18061 gatgaaagaa gaatcagcac tatctaattt ttcttgaaag tattgagttg ttcatcctag
    18121 gcagaacgat aaggcaagtc ttggttttta ttccccttga aaaagatcgt ttaataaata
    18181 tgttctgtat aaaaatacag tatattgaaa aataacaatg gaagtagagg tgcagagaat
    18241 gaagaaaaga atgagaatca tcttagcttt aagtgttatg ttattcttct tgacggcttg
    18301 tgggcaagaa gatgacaaaa aaatcacgct gaacatggct tggtggggtt cgcaagtccg
    18361 acacgatgca acagtaaaag taatcgagct ttatgaagaa aaaaatcccc atgtgacgat
    18421 cgattatgaa ttttatgatt atgaaggata ttacacaaaa ttaggtacac tagcagcatc
    18481 agatgatatc tgggacattt tccaactgaa caatcaattt cctcaataca tcaatcagat
    18541 tgaaccatta gatgaatatg tggagcaagg gataattgat atttctaatg cagatgaatc
    18601 ctatttagca acgacgactt acgacgggca attagttgga ctgtctaacg gggtgaattc
    18661 gtttgcgatt gcttacaata aaaatatatt tgaacaatta aatatccctg aaccagcttc
    18721 aaattggaca tgggatgaat ttgaacaaat cagctatcaa atcactgaag aattaggaat
    18781 ctatgctatt tcaagattcg aagattttat agctggctgt attattcaaa taccacaagt
    18841 tgagaaagga ttgaattttt ataatcggga agatctatct aacagcttgg gatttgaaaa
    18901 tccagactat ctaacagatt ttttccagct gcgtaaaaat ctggtagaca aaggggccca
    18961 tccagaacca ggtggtggag ctgcaagtac gacggatctg tcttttcagg cggttaggga
    19021 tagagaagcg gcaatggtgt ttctatcaag taaccagttg acagaaatat tgtcaggggc
    19081 tcctgaagat atggagattc agttgattaa tccacctcgc agaaaagcag atggagaatc
    19141 tggtatccct ttacgttctt cgcaaatgct gagtatggca aaaagctcag cccataaaga
    19201 agaagctgct aagtttattg atttctttca aaacagtgaa gaagccaatg aaattttaaa
    19261 aggagaacgt ggtgtaccaa tcatgtcgaa tattcgagaa cagctcgctc gtgcagatga
    19321 tgcaactttg caagcgacat ttgattattt agaaatgatt tccgatatgg acaacggcga
    19381 agtgaatgtc tttgatagtg ctttgaatcc ggaaattcaa gaccaataca atcttttagt
    19441 tgaaaaagtc atttatgaag aagtaacagc agaaggaagg catgaaaaaa gaaaaactaa
    19501 aacggttctt ggatcaagac aatgtggtcg gttacgtatt tgcttctcca ttcatcctag
    19561 gatttctatt gataacagtg ataccgatga tcttgtcact ttattattcg ttcaccgatt
    19621 atcgtttggg aaatccagtc tcatggatcg gcttggataa ttatataaag atgctgagtg
    19681 atgcccgttt tttgaactcc atccgagtca cttttctcta tgtatttgca tctgtacctg
    19741 tgaaactgat ctttggtcta ttgattgctt atcttttaac gaggaatatc aaaggaacga
    19801 ctttctttcg ttctttgtat tatgtgccta gtttgatcgg tggcagtatt tctatcgctc
    19861 tagtttggcg ggagttgttt tctaaaaacg ggttagtgaa tattttcctg gaacgattag
    19921 gcgcacctgc gctttcctgg tttagcgatc aaaaactggt catgatccct cttgttttga
    19981 tgtctgcttg gcaattcggt tcggcaatga tcatctttgc tgcaggtatc aaagagatcc
    20041 caggctcgta ttatgaagcg gcagagatcg atggagcgaa caaatggcag acgttttaca
    20101 agatcacgtt acctcttctt tcgccgatca ttttgtataa cttggttatg cagacaatcg
    20161 cggcgtttat gacttttacg caggcattta tcattaccca aggtggtccg aatgatgcca
    20221 cgaactttta tgctctttat gtgtataacc aagctttttc ttttaattct atggggtatg
    20281 ctagtgctat ttcgtggatc atgctgttga ttatgggagc tgtcaccttt ctgattttca
    20341 aatcatcaaa aaaatgggtc gttgtgtgaa tgagaggagg aagaaatgaa taatacagta
    20401 acgaaatggt ttcaatacgg ttttattttg ctattaggtc tagtcatgat ttatccggtc
    20461 ttatggatga tcgcaggttc ttttaaaacg gatcaagaga ttatgagtgg ttcgctgaat
    20521 ttgattccag agacttttca atgggaaaat tacgcaaaag gatgggcggg gtttgctgga
    20581 atttcatttt ttactttctt taggaattcg tttgtcatct ctgcagtttc gacgataggg
    20641 acagttctga gctcgacttg tattgcttac gcattatcga gaatcgattt caaaggaaaa
    20701 agattctggt tcgtcgtcat gttagtaacg atgatgattc ctgcacaagt gattttgatt
    20761 ccgcagttta ttatttacaa tcggctgaat ttggttggga cgtatgtgcc gctgatttta
    20821 ccacattttt ttggacaagc atttttcatt taccagatca tgcaattcat ggtcagcatc
    20881 cccaaagagt tagatgaatc agcgattatt gacggatgca gcaagtatag tgtctttacc
    20941 aaaattattt ttcctttgct gaaaccttcg atcatcacga cgatcatcat ccagttttat
    21001 tggaaatggg acgacttcat gggaccattg atttatttaa acaagccgag atcttacacg
    21061 gtatcgattg ccatcaaatt atttgcagac gctggatcta gtacgactta cgcgagtatg
    21121 tttgcgatgt ctacgttgtc gctgctaccg gtttttctga ttttcttatt cttcaataaa
    21181 tatcttgtcc aggggatcag tacaagcggt ctgaagggct gaatatgaaa aagccaatag
    21241 aagagatgtt tctttacaag ctcaactact atgtctgtcg gattttttta accagtctta
    21301 tcagtaatgc tcttttcgct ttagttctag gatgtttgtt cactttagga ttagtgctgt
    21361 ctgctcgtgt tgtctatctg atttattttt tgatgatgta tccttcttat tatctatctg
    21421 tccattactt ggcagaaaaa aaggtatgtt ggaggaaagg gaaaatcaaa cggatgtgtt
    21481 tcatcattac gttgtttcag ctgtttgtca ttatttttgc agttgattgg tatttattag
    21541 tgggaaagat tggagcttgg tatctgattc cttttctatt gtgtctgttg ctttttgtat
    21601 cccatagttt ttttagtacg ctaattgata tctgcaaaga agataaagca gatagtcttg
    21661 tttatttgat caaaaggaat atctggctgt tgtcaaaaaa cgtcaagggg tttctgtggt
    21721 atttatgcct tttgtatgga ctgttctggt gtatacagcc tgatttttta ctcactgcat
    21781 tactgtatcc agggctgcca tttgtcacag tattttgctt aaaagaaaag gagaatgact
    21841 ttaaatgaag aaaaaatatg tacaagtcgg acttggtggg agagcgcgtt ttttttatga
    21901 ggcaattgcc tctacgtatc atcaaacttg tgaactagtc ggtttttgtg atgtatctca
    21961 aacaaggatg gattatgcaa atcggatact tgtgaacaag tatggacaga aaaaagtaaa
    22021 gacatgtatg ccatcacaat ttgagcaact actggatgac gtacggccgg ctgaagtcat
    22081 tattacgtca gtagatcgaa cgcatcatga ttatatcatt cgtgcaatga aaaaaggata
    22141 tgatgttatt tgcgaaaaac ctctgactat cgatgaggaa aaagctcaag aaataataac
    22201 ggtccaaaaa cagacgggaa gaagcctgcg agtcactttt aattatcgtt atgcaccgta
    22261 tcacacgaag ataaaggaac tgatagaaag tgatgtgatt ggtgatgtgt tttctatcca
    22321 cttcgaatgg ctgttaaata cgcaacacgg ggcagattat ttcagaagat ggcatcgaaa
    22381 taagcacaat agcggcgggc ttcttgttca taaatctacc catcattttg atctagtgaa
    22441 tttttggttg aacagccgtc cccaaaaggt atttgcattc ggtggattaa acttttatgg
    22501 gatgcataat gcggagaaac gcggagtaga aatattttac agtcgatgct atggaagcga
    22561 actagctaaa gctgatccat ttgctataga cctgtcagcc aatgaggaat tgcgagaaat
    22621 gtatttagaa gcagaaaaag acagtggata tattcgtgac cgcagtgtat tttccgatga
    22681 tatctctatt gaagatacac taggcgtaac ggtcaaatat gaaactggtg cgattttgac
    22741 gtattcgcta aacgcgtata tgccgtggga aggattcaat gtttctttca atggcagcaa
    22801 aggacggatc gaaatgaaag tgatcgaaaa gtcttacatc aatgcaggtg gagataaaaa
    22861 ggatgaagga gcagccgttc ttcagtccat ccatgtctat ccgatgttcg gtgagccata
    22921 tgaggtaatc gtcgaacaag aagcgggcgg acatggcgga ggagatccgc gtttattaga
    22981 tgatctattt ggtgaagaaa aagaagaaga tccgtggaat cgggcagcaa ctcacgtaga
    23041 tggtattttg tcgattttga caggaatcgc agcaaatcat tctattgctt caggaaaagc
    23101 agtagtgatt gacgagcttg tcagcttaaa agaataagaa tagatttacg gaaattactt
    23161 cccgtagttt ataaaatctt gccttattgc cgataggctg tcttttgaac actatttatt
    23221 cggaaaaagg ggctatgaca tgctttttgt catgccttct ttttatgtgg gctgcttttt
    23281 tagataggag aaaataaaaa taactattta aacccgaaca aaaaggaata atgcattaaa
    23341 aatcattaga ttttacaaaa aaataagtaa accacgttgt cagtttcaga aagtagtgtt
    23401 agactgtttt caatatttga aacaacattt tttattcaaa tatggcaaac tgccttcacg
    23461 tgcaaaacag ttgcaagaaa atgaatttac actattatta gttttacata cctacttata
    23521 tatcgtcata atatggatga cagtttctag ccagtaccgt aaatgctgga ctataagtaa
    23581 aagattggct atttcaccct tcttttctta tgataaagtg gagggtgttt ttctttaaca
    23641 gaaacatagt aggtttttgc acaaaagcgg ttatttcggt attaataaat gataaaggag
    23701 caaataaagt gaaagaatta gttgaacgta ttcaaaaaga cggacgtata ttaggcgagg
    23761 gcgtattaaa agtcgacagc ttcgtcactc accaagtaga ccctgtattg atggaacaaa
    23821 tgggaaaacg gtttgcagaa gtatttaaag aacaaggaat cacgaaagtt gtgaccattg
    23881 aagcttctgg gatcgcacca gctttatttg ctgcaagaga attagatgtt ccaatgatct
    23941 tcgctagaaa agcaaaaagt ttgacgatgg atgaagaatt attgacagct tctgtttatt
    24001 cttttacgaa acaagtgaca agtacgattt ctatttctcg taaattttta tcttcagaag
    24061 acaaagtatt gattatcgac gatttcttgg caaacggaca agcggcaaaa ggattgatcg
    24121 aattatgcca gcaagctgga gcgcaagtag aaggaatcgg gatcgtcatc gaaaagtctt
    24181 tccaagatgg acgtcagtta cttgaagaaa tgggattgag agtcgtttct cttgcgcgta
    24241 ttgcttcctt gacgaatgga caagttgagt ttcttgagga ggacgcataa tcgtggaaaa
    24301 agaaacacaa aatggaaaag ctgcggtttt agggctgcag catctgctag cgatgtatgc
    24361 aggagcagta gcggtgcctc ttttgatcgg tacaggacta ggctttaatc aagaacagat
    24421 gacttatttg atttcaattg atatctttat gtgtggatta gcgacattat tgcaattgac
    24481 agttaaccga tttttcggaa ttggcttgcc tgtcgtttta ggatgtgcga tccaagcagt
    24541 agctccactg atcttgatcg gaaccaatga aggagtagga gctatctacg gatctattat
    24601 cgcttctggg atctttgttg tgttagtttc tggtgtcttt tctaaaatta aaaaactatt
    24661 ccctcctata gtaacaggaa cggttatcac cgttattggt ttgactttga ttcctgtagc
    24721 aatcgagaaa atgggtggag gtaatgcaac agcagctgat tttggtgaca aaacaaatct
    24781 gctacttgca tttgtgacga ttctcctaat tgtaggcgtg caaatgctgg caaaagggtt
    24841 cattcgttca atcgcggtat tgattggttt agtcggagga agcatcttag ctgctttcat
    24901 gggaatgatt gatctgagcg cgattggaga tgcgccgttg ttccatgttc cacaaccctt
    24961 ttattttggt aaaccaactt tcgacgtatg gtcgatttta ctaatgatca tcatttccat
    25021 ggtcagcatg gtagaatcaa caggtgtata ttttgcactt ggtgacatta ctgggaaaaa
    25081 tgttggagaa gaagaattga aaaagggata tcgtgcagaa ggacttgccg ttatcttagg
    25141 cggtattttc aatacattcc cttatactgg cttttctcaa aatgtcggat tggttcaact
    25201 ttcgggaatc aaaacaagaa aacccatcta tttctctgca ttcttcttga ttgttttagg
    25261 attattccct aaaatcggtg cgttagcaca aatcatccct gagccggtac ttggtggagg
    25321 aatgttagtc atgttcggga tggtggcagt ccaaggaatc cgtatgctgt tggaagttga
    25381 tttcagcaat gataaaaatc tattgattgt tgctgtttct gttggatttg ggctgggttt
    25441 caatgttacg ccgacattat tcagtcaaat gccgcaaaca ttgcaaatgt tcactggaaa
    25501 tggcatcgta atgagtagca ttacggctat catattgaat ttgatcttta atggattgaa
    25561 aaaagatgaa gaaatattgg aagaagcctt caagaagtaa tagagcaaaa gaaaggttgt
    25621 gaaatcagcg ttttgacctg attgttagag aaacaaacgg aaaaatagtt cctcgtattt
    25681 tgccgtttgt ttgagctaat accgagggtc agctgattga acaccgttta tccataaaaa
    25741 ggaatgtgac ctaacttttg tcacattcct ttttttcatc tttaataaac acgaacaata
    25801 aaatgttttt ctttgttaac attcgaaatt tctttgacat caaaaaaagt gattgttaaa
    25861 atatgaatgt agaaaaactg ggaggaaaaa caatgacagc aaaaatctct gtgattatgg
    25921 ggagtacttc agattgggaa acaatgaaag aagcatgtca aatattggat gaattcggtg
    25981 tgtcttatga aaaaaaggtc gtttctgctc atcgaacccc agatttgatg tttgaatatg
    26041 cacaaaacgc tcgaaaaaaa ggaatcaaaa tcattattgc aggagcaggg ggagccgcgc
    26101 atctgccggg tatggtcgca tcaaaaacaa ctttgccggt tatcggtgta ccggtccaat
    26161 cacgaacatt aaatggtttg gattcgcttc tttcgattgt ccaaatgcct ggaggagtac
    26221 ctgtagctac tacagcaatc ggaaaagcgg gagcaattaa tgctggacta cttgcgatac
    26281 agatgctttc catgtatgat ttagaattgg aggctaaatt ggctgaaaga cgttcaatgt
    26341 tggctaaaac tgttatagaa agtagtgatc aacttggtta aacctttgtt gcctggttca
    26401 acgatcggga tcgtcggtgg cggtcaatta ggtcgtatga tggcgattag tgcaaaagaa
    26461 atgggcttta aagtaggtgt gctggatcct gtgagcgatt gtccagccgc acaagttgca
    26521 gattggcaca ttgaaagtgt ctatgatgac acctttgcgc tgaaagagct tgccagaaga
    26581 acagatgtca ttacctatga attcgaaaac gtcagtgtag aggcgttaaa tgcgatttta
    26641 cccatgtcat ttattcctca agggacagac ttgcttgcga ttacgcaaga ccgcctgtta
    26701 gaaaaatctt ttttggaaac gaataacatc gtgattgctc cttatgcaac gatcgtcagt
    26761 ccgacggata tccaagatgc gattgacggg atcggctatc cttgtgtttt gaaaaccact
    26821 cgaggcggct atgatggcaa ggggcagtat gttttgaaaa gtcgggcgga tctagctcct
    26881 gccatggacc ttttaagaga aggaacctgt gttttagaag catggatccc gtttgaaaaa
    26941 gagatttcga tcatggtggc aggaaatggt caaggagatt tcacgacttt tccggtcgtg
    27001 gaaaacatcc accacaacaa tattcttcat gaaacgattg ctccagccgc gattgatcag
    27061 gatgtgatcg aggaggctga gcgtattgcc cgtgtgattg cagaagcagt gagcctttca
    27121 ggtgttttag gggtagaaat gtttttaaca aaaacaggcg ggctatatgt gaatgaatta
    27181 gctcctcgtc ctcacaattc tggacattat tctattgaag cttgttctat gagtcagttc
    27241 gatgcccata ttcgaggtat ttgtggttgg ccattaggag aaaaagtacg tttgataagc
    27301 aatgcagtaa tggtcaatgt tttgggagaa caactgctgg aaagctatcg cttgattgag
    27361 aataagccag actggcaatt ccatttctat ggaaaaaaac aagcaaaaaa aggtcgcaag
    27421 atgggacata tcactatctt gacagaagac atccagcaaa cgctggaaga aatcaaacaa
    27481 acgaatattt gggattagag gagaaaaaac atgttagaac gttatacacg ccctgaaatg
    27541 gggaatatct ggacggaaga gaacaaatac cgtgcgtggc tcgaagtcga gattttagca
    27601 gatgaagctt gggcagaact tggtgaaatc ccgcaagaag atgtgaaaaa aattcgtgag
    27661 catgcatcat ttgatatcga tcggatcttg gaaatcgaaa aattaacacg ccatgatgtc
    27721 gttgcgttta cacgagcggt ttctgaaaca ttgggagaag aaagaaaatg ggtccattat
    27781 ggtctaacaa gtacggatgt cgtagatact gcttatggct atctcatcaa gcaagcaaat
    27841 gatattttac gggaagactt gaaacgtttt acggaaatca ttggcgaaaa agcaaaagaa
    27901 cacaaatata cagtgatgat gggtcggaca cacggtgtcc atgctgaacc aactactttt
    27961 ggattgaaac ttgccacatg gtattcagaa atgaaacgga acatcgaacg atttgaacat
    28021 gcggcaaaag gagtagaagc aggaaaaatc tcaggagcag tgggaacgtt cgcaaatatc
    28081 ccgccattta ttgaagagta tgtatgtaaa caattgggga tccgtccaca agaaatctct
    28141 actcaagttc tccctcggga tctacatgct gaatatttct cagcgatggc gcttattgct
    28201 acaagtattg aacgttttgc aacagagata cgcggattgc aaaaatccga aacacgtgaa
    28261 gtagaagaat tttttgcgaa aggtcaaaaa ggttcttctg ctatgccgca caaaaggaat
    28321 ccgatcggtt cagaaaatat ggctggtcta gccagagtgg tgcgcggaca catggtcact
    28381 gcctatgaaa atgtcacatt atggcatgaa agagatattt ctcattcttc agcagaacga
    28441 atcatcttgc ctgataccac gattttattg aattacatgt taaatcgttt tggcaatatc
    28501 gttaaaaatc taacggtctt tcctgaaaac atgaagcgga atatgtctgc aacatttggt
    28561 ttgatttata gccagcgcgt tatgctgaaa ttgatcgata agggaatgac aagagaagaa
    28621 gcttatgatc ttgttcagcc aaaaactgcg tacgcttggg acaaccaagt gcagttccgc
    28681 ccgctgttag aagaagatcc aaccatcaca tcggtattat ctaaagaaga aatagacgat
    28741 gcctttgatt atcatcatca tttgaaaaac gttgacgtga tattcaaacg agtaggtttg
    28801 gaataaagaa gagtgagttg caaaattcaa ttaaaataga atagcaaaaa taaccaaaaa
    28861 agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct tatcaataat
    28921 aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata caaaaaatag
    28981 aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg gagatgttgc
    29041 agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat ctggatacga
    29101 taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc gcctttgtgt
    29161 tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt tgctcggcgg
    29221 atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc tttgatcgac
    29281 agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa aaaaagctga
    29341 ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat ccaaattttt
    29401 agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat ggtataagat
    29461 tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg tacatatttt
    29521 tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac aaaaaacgat
    29581 gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa gaacttttgt
    29641 atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt tggatagaat
    29701 atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt ggaaaaggag
    29761 aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga ggaataaaaa
    29821 atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta caaattatcc
    29881 ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt ctaggtgttc
    29941 ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat gaccagttgg
    30001 atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaaa ccggaagaaa
    30061 tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa ttatttgcta
    30121 aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat ggacaaattt
    30181 tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt acgaatgaac
    30241 atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc tatcaagaag
    30301 tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt atttcgtaaa
    30361 agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag taaaaaaagc
    30421 agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat actttgagat
    30481 acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt gtgacaagct
    30541 gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg aggaagtata
    30601 acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat atgttatggg
    30661 cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag tcctctttag
    30721 aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat ttgagatgcg
    30781 gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct gaagaaggga
    30841 aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga cttttgccag
    30901 gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca ttgaaagtca
    30961 accccaagac ggcctttact tcagagtatg aagccgacga aataatctat cttccagttg
    31021 ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta aaaaaaaata
    31081 atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac attgcaggga
    31141 ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt gcagtagaag
    31201 acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa aacttcggaa
    31261 aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc aagaaaaaaa
    31321 gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa gaagatatct
    31381 taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg agcgaacatt
    31441 gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga cctcatgtgt
    31501 tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta gcagttgtct
    31561 tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga gctgcgacag
    31621 gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt gcgttgctag
    31681 attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta gaagaagtag
    31741 ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc ggcgaaattg
    31801 catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta ggtttgattg
    31861 aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc atgtatgtag
    31921 gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa gaatttaatc
    31981 aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg gaaaagctgc
    32041 tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata ggtatacagg
    32101 atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa gctggctcag
    32161 gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca ccttatgaaa
    32221 tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga gaggaagaaa
    32281 aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt aaagtaacag
    32341 acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg ccagtcgatg
    32401 cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct cgaatcgtag
    32461 aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa gtattgcttg
    32521 atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact tatgattcac
    32581 aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg agggttagag
    32641 ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg tatttggatc
    32701 caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt gctagcggtg
    32761 gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa ccagaagttt
    32821 tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg ttaggaactc
    32881 ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca atctatccaa
    32941 caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact actcaggaat
    33001 tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat tttgatggca
    33061 gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac tttgatctat
    33121 ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga ttgattgaaa
    33181 gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca gctttcaaac
    33241 atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt gcagaaactc
    33301 aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa gaaatgatgg
    33361 gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa atttcagcaa
    33421 ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag gatgcaatcg
    33481 tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg tttggggtca
    33541 tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc gaggacaaga
    33601 aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag atttggggct
    33661 gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag aagctgcaat
    33721 cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc agccttttct
    33781 attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga tcaatgctcg
    33841 ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact ccgatacgga
    33901 gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata aattaaaaag
    33961 tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa cggcaatgat
    34021 tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga aaaacggagc
    34081 atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt ttataagaga
    34141 tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg attgctttac
    34201 gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca gaccagattc
    34261 aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga atttagcgaa
    34321 agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt tgtcagccgc
    34381 aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa aaaatcaata
    34441 cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg ttcgaatgaa
    34501 attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg atgattcaat
    34561 tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag gggcaaaaga
    34621 agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg ggattgatat
    34681 tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca aagaatgtat
    34741 cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga tcggtctgga
    34801 aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt atccgacgcc
    34861 gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat cgttctttta
    34921 aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca aaagctggcg
    34981 tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc aaaaaaacgg
    35041 aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat ttatcggtct
    35101 ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg aaattagcag
    35161 tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg atgtgtgtca
    35221 atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta gcaacgggga
    35281 aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga tgtgttcaag
    35341 gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac tcaggtaatg
    35401 attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta ttgacaggag
    35461 aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt cattcaaatg
    35521 ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta tcatcaaaac
    35581 tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa atctatgttt
    35641 cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat atcactggtg
    35701 gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca caaatcaaac
    35761 ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt gaaattcctg
    35821 agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca gtcgaagaat
    35881 cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt tatgaaatcg
    35941 ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa cgatgagaat
    36001 cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt atctatcgaa
    36061 aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag catatgtttt
    36121 gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag aatttgattc
    36181 aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga tcgatttgat
    36241 cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt atgataaaag
    36301 aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg gcatacgtga
    36361 tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg accaaggtgt
    36421 agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg acacgttcga
    36481 ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag tgattagtga
    36541 aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt atcagataaa
    36601 actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat catttcaaca
    36661 ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat cgaagaagtg
    36721 acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa aatccatggt
    36781 ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac ggaaaatatc
    36841 caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat tatgaaacca
    36901 gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag tatgctccgt
    36961 agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga ctatgaagaa
    37021 gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca aaaactagcg
    37081 gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata cttaacggat
    37141 tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa acaaacgctt
    37201 cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct tctggaaaaa
    37261 tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa taatatccga
    37321 gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt agtcgccttg
    37381 aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc gtgggaagca
    37441 gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa tcgagaagta
    37501 gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat tgccccttct
    37561 tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt actgacagta
    37621 gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt aggtggactt
    37681 cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt cgtgacaaaa
    37741 cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc tgtcaaacat
    37801 gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat cggagccggg
    37861 cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga gaaaatggaa
    37921 ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga atatgcagcc
    37981 aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca agattctatt
    38041 gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca ttttagacat
    38101 tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg tgaacatgca
    38161 ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc gccaggaaat
    38221 ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga tcattcagcg
    38281 ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc tgaagttcct
    38341 ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt cggtccgaca
    38401 aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat ggatacttat
    38461 caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc agcgtatatc
    38521 aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg gaaaggtgta
    38581 gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt gaaccagcat
    38641 cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg agaagaattc
    38701 tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc gcaagatcat
    38761 aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc ttattcacca
    38821 gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt aaaaccagcg
    38881 gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc gggtttgatt
    38941 gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga tcctgaaacg
    39001 caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga tttattagag
    39061 aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt tgtggttgca
    39121 gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt tctggaagaa
    39181 gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt tggagcagga
    39241 ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca gaaaaaaatt
    39301 tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga tattggagaa
    39361 aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt ataaaaaata
    39421 caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga gtgtggtaca
    39481 cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat taggtacttc
    39541 tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg cggaagatcc
    39601 agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc atgcagatat
    39661 ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa catcaatcaa
    39721 acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga tgtttgattt
    39781 ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga aaatggatta
    39841 tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg aagtagctga
    39901 agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca gcaatcaaaa
    39961 accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag ctaatcagct
    40021 gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca atatgacaga
    40081 tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc atgatatgac
    40141 gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta tcgggaacga
    40201 aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc aaacgacacc
    40261 aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag tgatcgccgg
    40321 tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta atatgtctcg
    40381 ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa gattgtcata
    40441 agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg ttttcagctt
    40501 cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg gatttcgttt
    40561 attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga gaaatccctt
    40621 gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt tcaaaagcaa
    40681 tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg attctttgtt
    40741 ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta ctgaaatgag
    40801 agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga tcggtaaaat
    40861 tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc cttgaaggct
    40921 gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg taaagcgagt
    40981 cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt tcttgcggga
    41041 ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat aaacgatata
    41101 atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac ttagatactt
    41161 cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca gaatcaactg
    41221 gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta agggaactgg
    41281 cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg attctttctg
    41341 cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat tcatggacca
    41401 acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa tcaactatta
    41461 aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc tccctaaata
    41521 aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg acttggtttg
    41581 ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat tcagcaagtt
    41641 tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa tgattttttt
    41701 tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa tctaaagcag
    41761 ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta agcagataat
    41821 catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat ccactaataa
    41881 atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc aacccgtcca
    41941 tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata attgaagaca
    42001 gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa gaaccaagga
    42061 cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg ataaaaactt
    42121 tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag tgtgacagtt
    42181 gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc gtaataaaga
    42241 aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga atcgatccct
    42301 ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat cccgttgtct
    42361 ataactttga attggatcat ctcatcaatt ttgcatgcgg taatacgaat aaatcctttg
    42421 tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg caagatcatt
    42481 tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc aagcatatgg
    42541 ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc tttttcaaga
    42601 ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag tgcatgggtc
    42661 atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc atccaaactg
    42721 ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact ttgtgctaaa
    42781 tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc cattttattc
    42841 atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg aaagccaagg
    42901 ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc ttggaccttt
    42961 ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag aactccaagt
    43021 tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt gcttgctaag
    43081 tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt gacagaatct
    43141 acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat attattgatc
    43201 agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc taacgtacta
    43261 atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct aagttcttct
    43321 aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa gtcccacata
    43381 ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg gatatttgcg
    43441 atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag tagcaaaggg
    43501 ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt tatgcttaaa
    43561 ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag ctatattctg
    43621 ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc ctgtttgaat
    43681 atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag gtttacactc
    43741 gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt gtccatcaat
    43801 agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg cagctaaagc
    43861 accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata aatcatttgt
    43921 ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac cgacaattcg
    43981 gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta gggttggttt
    44041 acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg agttctctga
    44101 accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt gattgctgac
    44161 aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg tgacgaaaag
    44221 ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc ctgctcgctt
    44281 tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa tgatcatatc
    44341 cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat tcatgtagcc
    44401 atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg attcagtatg
    44461 ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta gtgggtcagg
    44521 tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa caagggctaa
    44581 aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt actatcttat
    44641 cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca agaaacttaa
    44701 aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca taaaaaaatc
    44761 aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg gggataaaaa
    44821 atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc aagcattcta
    44881 ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt aggaatcgct
    44941 atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt ctcggaatta
    45001 gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga aaatcaagta
    45061 gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc ttctatcgat
    45121 ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa ggtgatcgcc
    45181 tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt tgataatttt
    45241 ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa agaaggaaaa
    45301 ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc actaatcaat
    45361 tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt ggtcgtgcca
    45421 tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc tacggcgcaa
    45481 gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga tgctgttttg
    45541 tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt ggggtatggg
    45601 agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc tggcgttaaa
    45661 tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat cttagcaaaa
    45721 aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt caatgatact
    45781 gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac agtatctgtc
    45841 gataaagata attatcaagc agagctgatc gacaccgatt attataaaga atccgatctg
    45901 aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag aggataagaa
    45961 ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct ggaatccgag
    46021 cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg tgcggagaaa
    46081 acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca tatggctcct
    46141 atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg aaagatagtg
    46201 aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat ctttcaataa
    46261 aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat tgggaactga
    46321 cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac ccgaatcttc
    46381 tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa gcattatcaa
    46441 aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa gaagaaagcg
    46501 cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc atcattattt
    46561 cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta cgggatggac
    46621 agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt cgcgggatgg
    46681 tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga gaagtttatt
    46741 ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc ataaacgacc
    46801 acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg atgggagcgg
    46861 gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag atatccggac
    46921 gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc attgcaaacg
    46981 gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg atggatattc
    47041 gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta ttagataaag
    47101 aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa acaaactcca
    47161 tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt ttatccaagt
    47221 ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg atagatgttg
    47281 gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc aaagctgtct
    47341 gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc tatacgatga
    47401 acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa gtcttgatga
    47461 aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct tacggataaa
    47521 aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg ctacagcatg
    47581 gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg tattttctgg
    47641 cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt actagctgca
    47701 gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc agtgatggcg
    47761 tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc gtggatgaca
    47821 gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt ttgggtagct
    47881 tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt tcgtggactg
    47941 acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt ccaaaaaatg
    48001 tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt gacattgatt
    48061 ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag aaatcgtcgg
    48121 aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc ggtatttaca
    48181 gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta tccgattatt
    48241 ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa aacagtagca
    48301 ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc agggatcaaa
    48361 acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc tttagctgga
    48421 ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc gatggaactg
    48481 gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat tggaacaatc
    48541 atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat gtcgatttta
    48601 ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt agccgttgtg
    48661 ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta ttcagaaaaa
    48721 agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca ttcattgttt
    48781 cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat tctctttttg
    48841 ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa agccttgatt
    48901 aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg taatgaaagt
    48961 atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata gccgttctga
    49021 atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag tgcctgccaa
    49081 catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg gttatttcta
    49141 tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa tgaaaaagcg
    49201 tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct ttatagagaa
    49261 gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc atggacatgc
    49321 taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa cttttgtgat
    49381 tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg gcgcagatgc
    49441 aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga caggctttgg
    49501 gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc gcaagccgat
    49561 catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc gttttggtgc
    49621 gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg gcgaaacaaa
    49681 agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat tccaaaaagg
    49741 cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat ctttgaaaga
    49801 tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg gaggaagaga
    49861 tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt ttaatggaga
    49921 cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc tgcattaagt
    49981 aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg aattattatc
    50041 gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa gatttttgtc
    50101 acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc ggtaaaatca
    50161 aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga gaaaaaacaa
    50221 ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa cggaaggatc
    50281 aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg tgtgaatcgt
    50341 tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac gagcaaacgt
    50401 ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc aagaatcgat
    50461 tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca aaaaataaga
    50521 gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga gatgccagat
    50581 gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt agcggaagaa
    50641 aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg gtatttggaa
    50701 agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg cggtcagcaa
    50761 gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc tgtagagtca
    50821 ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt atttgggata
    50881 ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca taaaaataaa
    50941 ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt gatgagtcaa
    51001 aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat tattgaagac
    51061 gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat acgaacgatt
    51121 gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa aacaacgaaa
    51181 atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc gcaagaaatg
    51241 atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt taatgaagca
    51301 attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg actgataagc
    51361 gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg ttactatgcc
    51421 tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt agtagatatg
    51481 ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat cagaatcaat
    51541 tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa aattctagaa
    51601 aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac agcttggatt
    51661 taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga aactaagaaa
    51721 aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt gcaacaaaac
    51781 tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt agaaaataga
    51841 aaaaatgcag atgaaaaata aaacgctttc cttattgtga ttttattcac taatgctgtt
    51901 aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta gcttgtattt
    51961 ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca gttttattca
    52021 ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg tgtgacaaat
    52081 ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca atgcagattt
    52141 cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt tagtacctga
    52201 tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc gtgtattaga
    52261 ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac caactgctgg
    52321 acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg ttcttcttcc
    52381 aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc aagctttttt
    52441 atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag ctctaccacc
    52501 acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag gtttgaagaa
    52561 acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat cacaaggtga
    52621 tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat tttatatcca
    52681 aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa aaacacttgc
    52741 acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg atccgcttgc
    52801 agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg gaccagtcct
    52861 aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg atccaacccg
    52921 ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga tccgcttccg
    52981 taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag tcatcgaagc
    53041 aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa aacaaaaggt
    53101 ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag aacaaattgc
    53161 aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat ccaagcaatc
    53221 acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt tggtgaagat
    53281 gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa gttcggagaa
    53341 gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag tttcggtctt
    53401 gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat tttcgaagca
    53461 atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg cactagaaat
    53521 ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga attgcattcg
    53581 gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat tccttccaac
    53641 ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc ggttgttttc
    53701 ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga agcttacgaa
    53761 gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat tatcacatac
    53821 ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga aaatatatct
    53881 gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat cattcaatcc
    53941 gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc gggcgttgcc
    54001 gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc cccaatcgga
    54061 cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt ctggttgcca
    54121 aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga aaagaaggga
    54181 cgaaacaaag tcatggctta tcaatttaaa ttgccggata tcggtgaagg aatcgcagaa
    54241 ggtgaaatcg tcaaatggtt cgtcaaacca ggagatacga tcaatgaaga tgatacatta
    54301 ctagaagttc aaaatgacaa atcagtagaa gaaatcccgt cacctgttac aggaacggtt
    54361 aaaaatgtga ttgttccaga aggtaccgta gcaaatgttg gggatgtatt agtagaaatc
    54421 gatgcaccag gacacgaaga caatgaaggg gacagtggtg tagccgcaga atcccaaact
    54481 ccagctaaac cggctgctga acccactgtt gatacagaat cagctggatc gtcaagcgaa
    54541 ggagtcttcc aatttaagtt accggatatc ggtgaaggaa tcgcagaagg tgaaatcgtc
    54601 aaatggttcg tcaaaccagg agatacgatc aatgaagatg atacattact agaagttcaa
    54661 aatgacaaat cagtagaaga aattccgtca cctgtcacag ggacagttaa aaacgtgatt
    54721 gttccagaag gcactgtagc aaatgttggg gatgtattag tagaaatcga tgctcctgga
    54781 cacaatagtg caccgtcaac tagtgcgcca tcagctgaag caccaaaaga aaaagtggaa
    54841 acatcaggtt cagcaagtgt cgttgaagct gccgatccaa ataaacgtgt attggctatg
    54901 ccatctgttc gtcaatttgc ccgtgaaaaa gatgtagata tctcacaagt aactgcaact
    54961 ggaaaaggtg gcagagtaac aaaagaggac atcgaaaact tcttggcagg tggtccaagc
    55021 agtgcaccag cgaaatcaga agcgccagaa gcggcagcac cgaaagaagc agctcctgca
    55081 gcagaaagca aaccagctgc accagcaaaa ccattcaagt ccaacttagg agacttggaa
    55141 gaacgtgtag caatgacgcc aacacgtaaa gctatcgcta aagcaatggt caacagtaag
    55201 catacagctc cacacgtgac attgcatgat gaagtagaag tgtctaaact atgggataat
    55261 cggaaacgat ttaaagaagt tgctgctgca aatggtacga aacttacttt cttgccatat
    55321 gtcgtgaaag ctttaacagc gactgttaag aaatacccag tattgaatgc atctatcgac
    55381 gatgcaaacc aagaaatcgt ttataaacat tactacaata tcggtattgc aactgatacg
    55441 gatcacggac tatatgtacc aaatgtgaaa gatgcagacc gcaaaggaat gtttgctatt
    55501 gcagacgaaa tcaatgagaa agcaaaatta gctcatgacg gcaaactatc tgcagaagat
    55561 atgcgcaatg gaacaattac gatcagcaat atcggatctg taggtggcgg ttggtttaca
    55621 ccagtcatca actatccaga agtagctatt ttaggtgtag gaacgattgc acaacaacca
    55681 atcgtaaatg ctgaaggcga aatcgttgtc ggtcgtgtga tgaaactgtc attaagtttc
    55741 gatcaccgta tcgtagatgg agcaactgcg caacaagcaa tgaataatat caaacgtctt
    55801 ttagctgatc cagagctatt aatgatggaa ggatgataaa caaatggtag ttggagattt
    55861 tgctgtagaa ttagatacag ttgtaattgg tgcgggacct ggtggctatg tagctgccat
    55921 ccgcgctgct gaaatgggac aaaaagtagc aatcattgaa agagagtata ttggcggtgt
    55981 ttgtttgaat gtcggctgta ttccttcaaa agcactgata gctgcaggac accattatca
    56041 agagtcacta gattctgaaa tgtttggagt aactagtgaa aacgtcaaac tagattttgc
    56101 aaaaacacaa gaatggaaag aaaacaaagt tgtccatacg ttgacttctg gtgttggttt
    56161 cctattaaaa aaacataagg ttgaaacaat cgaaggagaa gcgttcttcg tagatgatca
    56221 cacactacgc gtcattcatc cagattcagc tcaaacttat tcgttcaata acgcaatcat
    56281 cgcaacaggt tcccgtccaa tcgagattcc aggatttaaa ttcggaggac gtgtgttaga
    56341 ttctactggc ggattagcat tgaaagaagt acctaagaaa tttgtgatca tcggtggcgg
    56401 tgtgattggt gccgaattgg gcggagcgta cgcaaactta ggagcagaag taacgattct
    56461 tgaaggctca ccacaaattt taccaactta cgaaaaagat ctagtaaaat tagtagaaga
    56521 tgatttcaag aaaaaaggcg taacagtcgt aacgaatgca atggcgaaag aagcagtcga
    56581 taacggtgac agcgtgacag tgaaatatgc agtggacggg aaagaagaat ccgttactgc
    56641 tgattatgtg atggtcactg taggtcgtcg tccgaataca gatgatatgg gcttagaaca
    56701 agcgggtgtc gaagtaggag aacgaggatt gatcacggtg gataaacaag gacgtacgaa
    56761 cgtacctaac atctatgcca tcggtgatat cgttccaggg gcggcattag cacacaaagc
    56821 cagttatgaa gcaaaaatcg ctgctgaagc aatctctggc aaaaaagtag ctgtagatta
    56881 taaagcaatg ccggcagtag ccttcactga tcctgaattg gcttctgtag gtatgacgat
    56941 caaagaagca aaagatgctg gtatcgaagc aaaagcatat aaattcccat tttcagggaa
    57001 cggtcgggca ttatcgcttg gtaaaacaga aggattcatc cgtctagtta cgacgatcga
    57061 agataacgtg cttattggcg cgcaaatcgg tggagtcggc gcaagtgata tggtctctga
    57121 attagcttta gcaatcgaat caggaatgaa tgctgaagat atcgccttga cgattcatcc
    57181 tcatccatct cttggcgaaa tcgtcatgga tgcttcggaa ttagcattag gtttaccaat
    57241 ccatatttga tttgaataat acaggcgaaa cgcaactgaa aaatcagttg cgttttgttt
    57301 tttagaatta tcaataatat tattatgtaa acttaaaaat atgacaactt cttggtttat
    57361 taccacttcc tatgctacac tgaagaggaa tatgaaatag gagagttgaa gatgcaagaa
    57421 tatcagtatc cacttgattt agattggaca acagacgaaa tggtcattgt aatgaagatg
    57481 tgggaagatc tggaacgtgc gaatgaaaaa gggataaaca atgaagcttt tttgaagact
    57541 tatcagcagt ttaaaactgt tgtcaaatcc attggtgagg aaaaacgatt gggacgggaa
    57601 tttgaaaaag cttccggtta ttctttatat cgtacagtgc agcaagcaaa aaaaaatcca
    57661 aacaaaactt tgagaatgaa cgggtgagat aaatggaaca gatgattgat catatcaagt
    57721 cctggcttga agaagctggt gagaaaatca aatcttctca acaaacatta acaatctcgc
    57781 aaaaatcaaa tcgaacggat ctagtaacaa atgtcgatcg aaacatccag aattttctaa
    57841 tcgaaaaaat ccattccttt gatccagagg cgaaaatatt ggcagaagaa gaagggtgta
    57901 atcaattatc ggatctcgat ggaagagtct ttatcatcga tcctattgat ggcaccttga
    57961 attttgtgat tgaaggggaa aatttttgta ttatgctcgc tgtttatgaa tccggcgttg
    58021 gaaagcttgg tttcatttat gacgtgatga gaaatgaact tttttggggt ggaaaaggaa
    58081 taggcgtgta taaaaatgat gagccattgc ctagaccagc aaataaatcc ttatcggaag
    58141 gcttacttgg tgtgaatagt tatcttttcg gtcataataa atatggaatc cgagaaattg
    58201 cggaaagcag catggggaca cgtatgtacg gatgcgctgg tcttgaacta gtggcaatct
    58261 tgaaaggaaa ccacattgct tatatttcaa accttagccc ttgggattat gcagcgggaa
    58321 atgtcttatt ggaagagttt ggtatggtaa caagcggtct ttttggtact cctttgacat
    58381 ttgaaaaaag ggaatactat ttagcaggga cttcttgtgc atatgcagaa atcgcagacc
    58441 ttttgaaaaa agaaggctga aaaaaaggta gaaatcattc acaaaaatga taaaaacgag
    58501 caaaagatta attttctcga aaaaatgaaa acattttact atatttcatg aaaaaagtct
    58561 gctataatta gcagtcgagt aaaatttgcc ctgtcataaa gaaccaataa aaaaggagtt
    58621 agttacattg aattatagaa acgatatccg taacgtcgca attattgccc acgttgacca
    58681 cggaaaaaca acattagttg atgaactatt aaaacaatca gatacattag acgcacatac
    58741 gcaattgcaa gaacgtgcga tggactcaaa tgcgctagaa aaagaacgtg gtatcacgat
    58801 tttagccaaa aacacagctg tagactataa aggtattcgc gtaaacatca tggatactcc
    58861 aggacacgcg gacttcggtg gcgaagtaga acggatcatg aaaatggttg acggcgttgt
    58921 gttggtcgta gatgcgtatg aaggaacaat gcctcaaaca cgttttgtat tgaaaaaagc
    58981 actggaacaa catatcacac caatcgttgt cgtaaacaag attgacaaac cttctgctcg
    59041 tcctgagcat gtcgtagatg aagttcttga actattcatc gaattaggag cagatgatga
    59101 tcagttagac tttcctgtga tttatgcatc tgcactaaat ggtacgtcaa gcttgtcaga
    59161 tgatccagca gatcaagaac caaccatggc acctattttt gatacaatta ttgaaaaaat
    59221 ccctgctcct gtcgataaca gcgacgaacc attacaattc caagtttcat tacttgacta
    59281 caacgattat gtaggtcgta tcggaatcgg acgtgtattt agaggaacaa tcaaagtagg
    59341 agaccaagta gcattaatta aattagacgg aacagttaag aaattccgtg taacgaaatt
    59401 atttggtttc tttggactaa aacgtctaga gattcaagaa gcaaaagctg gagacttgat
    59461 tgctgtctca ggtatggaag atatctttgt cggtgaaaca gttactcctg tggatcatca
    59521 agatgcgtta cctatccttc acattgacga accaacattg caaatgactt tcttagttaa
    59581 taattcacca tttgccggtc gagaaggtaa attcgtaacc gctcgtaaga ttgaagaacg
    59641 tttgatggca gaacttcaaa cagatgtatc cttacgagtt gaaccaacaa actctccaga
    59701 tgcatggaca gtttctggtc gtggagaatt gcatttgtct atcttgattg aaaacatgcg
    59761 ccgtgaggga tacgaattgc aagtatctcg tccagaagtt attgaaaaag aaatcgacgg
    59821 tgtaaaatgc gaaccgtttg aacgcgtgca aatcgataca ccagaagaat atatgggtag
    59881 tgttatcgag tccttgagct tacgtaaagg tgaaatgcaa gatatggtcc atacaggaaa
    59941 cggacaaatc cgtttgacat tcttaacacc tgcccgcggt ttgattggtt attcaactga
    60001 attcctttca atgacacgtg gttacggaat catgaaccat acttttgacc aatatctgcc
    60061 aatgcttcct ggtcaaattg gcggacgtca ccaaggggcg cttgtttcga tcgatacagg
    60121 aaaagcaacg acttattcta tcatgagtat cgaagaacgc ggtacagtct tcgtagaacc
    60181 aggtacagaa gtatatgaag gaatgattat cggggaaaac agccgtgata atgatttgac
    60241 tgtcaacatc acgaaagcaa aacaaatgac aaacgtacgg tctgctacta aagaccaaac
    60301 atctgtcatc aaaaaaccaa aacaattgac gttggaagaa tctttggaat tcttgaatga
    60361 tgatgaatat tgcgaagtaa caccagaaag cattcgcttg agaaaacaaa ttttagagaa
    60421 aaatgctcgt gaaaaagcat ctaaaaagaa aaaataatct gtaaaaggat gaagagagaa
    60481 accagcttgt tataaaaaat agctggtttc tttttttgtc caactctttt ctgtggagaa
    60541 gtcatctttg tcaatggaca taaattcaga aaatacgccg aaagacgttt aaaagacttt
    60601 taaaaaactt atttctagag agtttttgtt tcataataag ataatgtttc acgaaaggag
    60661 atcaacttgt ccttcgaaga aaatcgaagt aaaatggcaa gagaaaggag cgggaagatg
    60721 aacgacttat taaaacaata ctttggttat gatgagttta gacctggtca aaaagaaatt
    60781 atccagaaag tcattgatca ggaaaatgta ttaggaatca tgcctaccgg aagtgggaaa
    60841 tcaatctgtt accagcttcc agctttacta ttagatgggc ttactgtggt tgtgtcccct
    60901 ttaatctctt tgatgaagga tcaggtggac gcggcgaatc aattaggtat acccgccact
    60961 tttatcaata gctcgttaga tggttacgaa acagctagac gcttccaaga aatcgataga
    61021 cagcagtacc gtttacttta tatcgctcct gaacgtttta tcatgccgga ttttatccaa
    61081 gcaatgaagc gatggaacgt ccgtatgatc gctattgacg aggcacattg tatctctcag
    61141 tgggggcatg atttccgccc aagttattta cagatggcga accagctaga tcaactgcca
    61201 aatcgtcctg taattgttgc tttgacagct acagcgactg tgcaagtagc tgctgacata
    61261 aaaagacttt taaaaattcc agaaaacaac catattcaga ctggttttga acgagaaaat
    61321 ctacgatttc aagtaattaa agatcaaaaa aaagaacaat atctaatcga atatttaaag
    61381 atcaacaaaa atcaatctgg tatcatttat gcagcaacga gaaaagaagt agaccgtctc
    61441 tatcatttgc ttaaaaaatt cgatttttcc gtgggtagat atcatggtgg tctaaatgag
    61501 aatgagcgaa cagaaatgca agaagccttt ttatacgatc gcttgcagct gattgtagcg
    61561 acaaatgcat ttggtatggg tataaataaa agcaatgtcc gttttgttat ccattatcaa
    61621 attcctggct ctctagaagc atactatcag gaagctggtc gggcgggaag agatggtctt
    61681 tccagtgaag ccatcctgct tttctctcct caagatatcc aagtccaaaa gttttttatc
    61741 cagcagtccc aaagagaaga agggcaaaag caaaaagaat atgaaaaatt aaaagcgatg
    61801 acagaatacg tgtatattga gtcctgtctt cagcaataca tcctaaacta ttttggcgag
    61861 acaagcagtc cgtgcaaccg ttgtggtaac tgtttagatg acagagaaat agtagaagta
    61921 acgacagaag cacagatggt tctttcttgc ttaaaacgta tgggtgagaa ctatggtaaa
    61981 caaatgctga tgaaagtctt agcaggatcg aaagaacaaa agctgcaagc tttaggtttt
    62041 ggacatttat ctacctatgg actgatgaaa aaccaatcgc aaaaagaaac gatgcagtta
    62101 attgaatatt tgatatcgaa tggctatctt ctgaccatca atggtgagta tcctgttttg
    62161 aaagtaacag aaagagggat acaagtattg aaaggacaag aatctgttta tcgaaaggaa
    62221 cccaagaaag tccagcagct atcggatgaa gaaaccgata cactatttga agtgctgcgg
    62281 gaactgcgaa cggatctagc atcagaagca ggcgttcccc cgtatgtcgt attttcagat
    62341 tccacattga aagaaatgag ccgaatacgt ccatcaagca gattggagat gcttcagata
    62401 aaaggagttg gccaaagcaa gcttgataaa tatggcgaag cctttttatc tcggataaaa
    62461 aatgcggact ctaacgttct ggataagtag aaagaacgaa tacgtaaaaa tgaataaaaa
    62521 caactggtta aatctgtttt ttttgctcct tatgaaaatc aaatatgtta taattaagga
    62581 attagaatgg gaggtaagca cagatggatt ctttgtcgaa aaaacttgcg atccaagtat
    62641 taaatgatga agcagaacgg atcaaacgat taatccgaaa tcaaaacaac agtctctgta
    62701 tctcacaatg caaagccttc gaagaagtgg tagatacaca gatgtacggc ttttcccggc
    62761 aagtcggttt tgctgtccgt ataggagtaa tcggaaagaa cgagggccag ctgatgttaa
    62821 gtgagctaga acgagaattg aatcagttat atagtgaggt ttaccaagaa atttatgata
    62881 aaaaagaagt tggcaaggag gaatagactt gcctaagaaa gtgaaaaaaa ggcatctatt
    62941 agactatagc atcttgattc cctatttaat tttatgtgta ttgggattga tcatggtata
    63001 tagttctacg tcgtatctat tattagaaaa cggacaaaat ccgtccgcat cagtcattaa
    63061 ccagagtatt ttttgggtgc ttagcttgat tgctatcgca ctactttata aaatgaaaac
    63121 agatgttttg aaaaaccagc gtttgatcat ggcagccatc gcagttttga cgattttgct
    63181 gctgattgtt gtgttttttg gtaaggagat caatggggcg aaaggttggc tgcaaattgc
    63241 aggattctcg atccaacctg ccgaatacct aaaaatcatt tctatctggt acctgtctct
    63301 gactttatct aaaagacaaa acagtgtaca aaaagatttt cttgggactg tgaaacgtcc
    63361 acttgcaatg gttatcggac ttactgcatt agtcgctatt cttcccgatt ttggtaatgc
    63421 ggcagttatc tttttgatta ttttggttct gcttttagcc agcggagtca attatgtgta
    63481 tactttgatt gtaggagtag gaggcttttg tctaagtact tttacgatct ggctgatcaa
    63541 tatcacaaac ggaaagatct tacccggacg tttacagtat atctataatc gatttgctat
    63601 ttaccaaaat cctttctcag acgaattaaa taaagggcat caactagtca acggctacta
    63661 tgccatgttc aacggaggac tatttggcag gggattaggt aacagtatcc agaaaaaagg
    63721 atttttacaa gaagcacaaa ccgattttat ttatgcaatc gtagtggaag aactgggagt
    63781 gattatgggg attttgatat tagcgttgct attctttatg attgtgcgca ttgtccttgt
    63841 aggtatccgt tcaaaagatc cgttcaattc gcttttgtgc attgggatcg gcgcaatgtt
    63901 tttgatccaa gtatttgtca atcttggagg aattactggc gttattcctt tgacagggat
    63961 cacgttccca tttttaagtc aaggaggctc cagtttgctg atgctttcga tttgtgtcgg
    64021 ctttgtcctc aacatcagtg cggatgaaaa acgcaagagc ttaggtttat aataaaaaaa
    64081 gatgtaggag tggttcgatg aaaaaagttt tagttgctaa caggggtgaa atcgctgtac
    64141 gtgtatttcg tgcttgtaca gaattaggaa ttaaaactgt tggaatctat gcagaagaag
    64201 atgaatattc cgtacaccgt ttcaaagcgg atgaagcata tttagtagga caaggaaaaa
    64261 aaccgattga tgcgtatcta gatattgaag ggatcatttc tatcgcgaaa gaatgcggag
    64321 ctgacgctat tcatccgggt tacggtcttt tgtcggaaaa cttgaatttt gcgcaacggt
    64381 gcgaagaaga aggcatcatt tttgttggac ccaaactgca tcatttagat atttttggag
    64441 ataaaatcaa agcaaaagct gcagctatcg aagcggggat tgcttcgatt ccaggcacag
    64501 atggaccaat cgcttctata gacgatgcct tggagtttgc gaaacaatac ggttatccga
    64561 ttatgatcaa ggcagctcta ggcggcggcg gacgcggcat gcgtgtagct cacgatgaaa
    64621 agagtgcaag agagggctac gaacgtgcga aaagcgaagc aaaagcagcc tttggtagtg
    64681 atgaagtgta tgttgaaaaa tacatcgcta atcctaagca tatcgaagta cagattctcg
    64741 gggatacgca tggaaacgtc atccatcttt ttgaaagaga ttgttctgtt cagcgccgtc
    64801 accaaaaagt agtagaagta gcgccttgtg tctctatgaa tgaacagcaa cgtcaaaaaa
    64861 tctgccaggc agctgtccaa ttgatgaaat atgtcgggta tgtcaacgca ggtacagtag
    64921 aatttcttgt tgaaggagat gatttttact ttatcgaagt caaccctcgc gtccaagtgg
    64981 aacatacgat cacagaaatg attacagata ttgatatcgt aacaacgcaa ctgttgattg
    65041 ctcaaggttt ggatctgcat aaagaaatcg gattgcctca gcaagaagga attaaattaa
    65101 atggttcagc cattcaatgc cgaatcacta cagaagaccc gttgaataac tttttaccag
    65161 acacagggaa aatcgatacg tatcgttcac ctggtggttt tggcgtacgt ctggatgtcg
    65221 gaaatgctta cgcgggctat gtagtgacac cgtattttga ttcattgcta gtaaaagtat
    65281 gtacgcatgg tgctaccttt gaaacagcca ttcagaaaat ggaacgttgc ttgaaagaat
    65341 ttagaatccg tggagtgaaa acaaacattc catttatgct caatgtcatc acacatcctg
    65401 aattccaatc aggaaatgca aaaacaacct tcattgatag tacagcaaca ttatttgaat
    65461 ttcctcgact acgagaccgc ggaaacaaaa cgatgaaata tatcggtgaa attactgtga
    65521 atggttttcc aggtatcgaa agtggcgaaa aaccatttta tgaagaacca cgtatgccta
    65581 aagatctaat cacaagaact gattacgtaa cggctaagaa tgtattagat gcgaagggtg
    65641 ccgatgcttt ggtcgaatgg atcaaaggac aggaaaattt gttgttgacg gatacgacat
    65701 ttagagacgc ccatcaaagt ctgctagcaa ctcgtgttcg gacaaaggat ttcaagcaga
    65761 ttgcacgttt gactggagaa ggtcttcctg aactcttttc aagtgagatg tgggggggag
    65821 caacatttga tgtggcttac cgctttttga atgaagatcc ttggcaacgg ttgagaaaaa
    65881 ttcgttcttt aatgcctaat acgttgctgc aaatgctttt cagaggttca aatgccgttg
    65941 ggtattctaa ttatcctgac aatgtattgg tggaatttgt caaagaagca gcagcgcaag
    66001 gaatcgatgt atttcgaatc tttgatagtt tgaactggac tccacaaatg gaaaaaagta
    66061 tccaagctgt acgggatact ggtaaaatcg cagaggcagc tatttgctat actggcgata
    66121 tcaacgatcc aagtcgggca aaatacaatg ttcagtatta taaagacatg gcaaaggaac
    66181 tagaacaact gggtgcgcat atcattgcaa tcaaagatat ggctgggctg cttaaaccgc
    66241 aggcagctta tcgtttgatt agtgaattga aagaaacgac agatctgcca atccatctgc
    66301 atactcatga taccagcggg aatggaatca tcacttattc tgctgcttca aaagcaggag
    66361 tagacatcgt tgacgtggca atgagtgcaa tgagtgggaa tactagtcag cctagcatga
    66421 gcagcttgta ttatgcactg gtcaatggtc cacggctgcc agaaatcacg attgaaaatg
    66481 cacagaaatt aaaccattat tgggaagatg tgcgcatgta ttacaaaccg tttgaaaacg
    66541 gcttgaatgc tcctgaaaca gaagtatata tgcatgaaat gccaggagga cagtattcga
    66601 atctacagca acaggcaaaa gctgtcggat taggtcatcg ctgggatgaa atcaaacaaa
    66661 tgtatcatac tgtcaacttg atgtttggcg atatcgttaa agtaactcca tcatctaaag
    66721 tagttggaga tatggcacta tttatggtcc aaaatgatct tacagaagaa gatatttatg
    66781 aaaaaggcga aacactaagt ttccctgaat ctgttgtgac tttcttccaa ggagaactag
    66841 ggcaacccgt tggcggattt cctaaaaaat tgcaaaagat tattcttaaa ggcagacctg
    66901 cattgaatga acgtccaggt cttttggctg aatcagtcga cttcaacgaa gtaaaaaaag
    66961 aattggcaga aaaaattggc tatgaaccaa aacaagacga agtattaagc tatttgatgt
    67021 atccgcaagt ctttttggac tatcaaaaag catataatca atttggcgat gttacattac
    67081 tggacacacc aacatttttc caaggtatac gtttgggtga aacaatcaat gtacaaatcg
    67141 aacgcggaaa aacgcttatc attcgtttag atgaaatcgg agaacccgat atcgaaggga
    67201 atcgcgtact gttcttcaat ttgaatggac aaagacgtga aatcactatc aaagataatt
    67261 cgatcatcag tacggtccaa acccgattga aagctgaacc gacaaaccga gaacaaattg
    67321 gagcaacgat gtctggttct gttttggatg tattagtgaa aaaaggcgat aacgttaaaa
    67381 aaggtgatac gttgatgatt actgaagcga tgaaaatgga aacggccatc gaagcacgtt
    67441 ttgacggaga agtcgcacat gtatatgtgt cttcaggaga cactatttcg tctggtgacc
    67501 ttttgattga agtgactgag aaataataag cgaaaggtgg ggaaacatga aaagactggg
    67561 tttgttttgg ctgattctgc tattagttat tgtggcattc tacatggaac cagtcttgtt
    67621 tccgccaaaa cttgaagtga aggaaaactc tgatcaacaa atatcaaacc aaatgacaac
    67681 gtggaaatac caggagctta aagcaactgg tttttctact tatatcggaa aaccgatcga
    67741 tcagctggaa aaggattttg gccaagccta tgatcgatta actagcggat ttggttttga
    67801 aacaagatat tatgaaaata aagctagtca tctcacattt gaagcgaaca tccaagacag
    67861 cagagtcaca actgttaaag tactgcaaac agggcctgct gatatcgcgc cgttttattt
    67921 agggatgacg atgcaagatc ttacgaaaat cactactatc tatactaatt tcacttttga
    67981 gtaccatgat acagaagttg gaatcgaact aatggaagag gatatgaatt atcgtccgct
    68041 aattgctttt gataacgaca cttttgctat tttatttttt gatcaaacaa caaacggcct
    68101 attttcagtt gtgtatttag ataaagatag tcttttgaaa ctattgccat atcaagtgtt
    68161 tggtgaaggc cttccacatt atcaagcaga taaatcggct gattgggaat caatcaatcg
    68221 cgttaaagaa cgaaaaagta cgactttatt aaatatgctt agaagagaag acgaacttcc
    68281 catatacaat caaacacttg cctttcaaaa ccacagtcag ctgctactac atgatttttt
    68341 gacaaggcct gaggaaatat tgtcagagga acgtatggct gattggcaaa aatcgctagg
    68401 cagtgctcaa acgacagtga aattctcact gacgaatgat gaattggaga agttaatcag
    68461 taaacaaaaa ttagaaaata ctagtgggct atttattcat ccggcgattg acgctacttt
    68521 ttcgttttta tattggtaca gcgatcctta caaccatgac cgtttcatgg acccgggaac
    68581 ggattctgta ggcattgctt tttctaaaga aaatatgtta gtcttattgc aagaagaaaa
    68641 cggagaaagt agtgatagca gtgatcgtta acgaagagct gtttcaactg gaggatcaat
    68701 gcctccaagt cgctgaaatg ataaaaaaga gtaaaacgat ggcttcttat cttgaacata
    68761 aacagtcaat ggaagaatct gcaaaagttt gccagttgaa agaagcgttc ttgtctaaga
    68821 aagaagcatt tgaaaatgta gcggcatatg gtgagtatgc acctgattat cgtgaaaaaa
    68881 aacgagctgt tcgaaaagca aaaagacagc ttgatatgga tgagcatgtt gcagctttcc
    68941 gatatagtga aacacagctg caaagtctgt tggatcaaat tgtccaagta cttgccgaag
    69001 aagtttctaa tgaaatcaaa gtagatgcag gtagcccgtt ttttgaaacg aaaaaacact
    69061 ttggatgcgg aggtaattgt catgcaatta gacaatagtg aatttgaaat aacaaaacga
    69121 cgcgggctga tcgtatgggt ctacagctta aaacaattaa agaatctgaa aaagttcggc
    69181 ttgttgcatt atgtctcaag aagaatgaaa tatgtcgtgc tttacttaaa tgaagagaca
    69241 ttggaagaaa cagaagaacg aatccgtaaa ttacattttg ttcgaaacgt agaacgttct
    69301 taccgaccgg atatcgaaat gaattttgcc gaaaaaatag gaaacaaaac agtattaaaa
    69361 gacgaaggtt tcgaaattga agagctaaat acagaaatcc gattagctga acatctttaa
    69421 aaatctctta tgagcagttt ttaaataaaa aataacttgg ataacgatcg atttttgatc
    69481 attgtccaag tatttttttg gtatactagt accagtacca tttctaagga gtgagtagat
    69541 gcggataata tcaggaaatt atggcgggcg ccgtttaaaa agtttagcag gggcgaacac
    69601 cagaccaact acagataaag taaaagaatc catattcaat atgattggac catattttga
    69661 tggagagaca gtattggatc tgtttgcagg aagcggtggt ttagctatcg aagcagtatc
    69721 tagaggctgc agtcatgcgg tatgcgtaga caaaaattac caagcgctaa aaatcatcaa
    69781 agaaaacatc gaaattacga aagaaccaga gaaatttacc acaataaaac ttgatgcgga
    69841 caaagcaatc gaacggctag cagcggagaa acaacaattc gactacttgt ttcttgatcc
    69901 gccatatgca aaacaaaaaa taatcgatca aatccataaa atggaagaac tgggactttt
    69961 ttctaaaacc gcagttatcg tatgcgaaac ggataaaaca gtcgaattgc cagaaactgt
    70021 ggctggattc catcaaataa gaaaacagat ttatggcata acggccgtaa ctatctatag
    70081 aaaagaggga aatcaatgaa acgtgcatta ttcccaggaa gttttgatcc ttttacaaaa
    70141 ggtcacttag atacggtgga acgtgcggct aaattattcg atgaggtagt aattggtgtt
    70201 tttatcaata cttcaaagaa aagtttgttt cccccagaag aaagaatgac attgatcaca
    70261 aaagctgttt ctcatcttcc taatgtaaaa gtcatgcatc aagaaaatca attgacggta
    70321 gaaacagcaa aagagattgg tgcagatgcg ttgatcagag gcatacgaag tatcaaagac
    70381 tttgaatatg aacgagagat tgctcagatg aatcatcatc tatatgatga attggaaact
    70441 gtcttcttat tagcaaaacc agaatatagt cacgtgagtt ccagtatttt aaaagaagta
    70501 ctgcactttg gcggagatgt gagcagctac ttaccgccag tgatcaacga agctttagct
    70561 ggaaagcgtg aatcaaatga aagctaaaca atggttgaaa acagtactga ttatcatatt
    70621 tggaatgttg atcattggat ttgtagtagt tcccttgccg tattatgtag aatcaccggg
    70681 agccacgatc gatctgaaag aactaattac ggtcaatgat aaggaagata aaggttctgg
    70741 gtctttctct cttacctctg ttggtatcag gcaagctaca ggtttcttag ctttaaaagc
    70801 taaaatgacc ccttttgaag aattgatcag tgaagaagaa ttgactggtg gggcgacaaa
    70861 tgaagaatat aatcaaatgc aacagtatta tatggattct tcccaaaatg cagcaatcga
    70921 acaagcgtta aaacttgcag acaagcctta taaaatggaa ttcaaaggcg tatatgtcat
    70981 gagtgtagag aaaacatcga atttctttgg caagctttca gttggagata cagtaacaaa
    71041 agtagacggg aaaagctttc aaagtacaga agcatttatg gattatgtaa aaagccaaaa
    71101 agttggacaa acaatcgaaa tcagttattt gcataatggt gaagaaaaaa cagcttctgg
    71161 tgatctgatc gagttaccaa cagataaaaa ggcaggaata ggtatcacgc ttactgacca
    71221 taccgagatc gaatcagata cagatgttcg tattaattct ggctctatcg gtgggccatc
    71281 agcgggattg atgttcactt tagaaatcta tgaacaagtc actggtaaaa acttgcgaca
    71341 tggtaagcaa attgcaggaa ctggaacgat caacagtgaa ggagaagttg gccgaatcgg
    71401 aggaatcgat aaaaaagtgg caagtgcgga taaagctggc gtagagattt tctttgcacc
    71461 agatgatacg atttcagacg atgttaagaa agaatatcct gaaataaaaa ccaactacga
    71521 agaagcaaaa gctgccgcta aaaaattgaa ttcctcaatg aaaatcgtac cagtaaaaac
    71581 agtacaagaa gcgcttgact atctagagaa aatgaaatga aactaaataa ttaaagaggg
    71641 gttgtgacac gagttctgtc acaacccctc tttttcgtca aaaatcttca cgataaaaat
    71701 aaaaacaaaa gaaaaaattg acgtaattag atgttgaagg gacgattttg ccagagctca
    71761 ctgtttcttc aattttaaat gaaaggagta atgaatgatt tttactggtc attctttgaa
    71821 aaaagaacaa tgatagaaaa aatcagcaag aaaatactag cttcacccaa attgatcatt
    71881 tggatgtcta tgatagttct tatcttttgt atcgcaagtg gttttttatt atcaaatttg
    71941 ctattttata gagaaacaga agagactgct gcaaaagaaa tactttcttc gtttagttcc
    72001 agtggagaag atccatcagc tgaaggaaac gatgtagaag aggatgctcg tccaaaggtc
    72061 atgtacaccg atatcaaagg aagtgtgaag gagcctggta tctattcctt ttcttcagaa
    72121 gaacgggtct atgatgtact aaagcgtgca ggtggattat tagaagaagc ggatagcgat
    72181 cgaatcaact tttctgcgaa gatagaagat cagcaagtcc tatatatccc tgctgtaggt
    72241 gaagagctgc cagaacatct tgatcagttg gcatcctcgg aaggaaaaca gcctacagca
    72301 gattccgagc ctcccaaaat aaatatcaat acagccactc catcagaact tcagcagatt
    72361 cctgggatcg gatcagtcaa agcgcaagaa atcattcgat ttagagaaga aaacggatcc
    72421 ttccagaaag tggaagacct gcaggagatt tctggtatcg gtgaaaagac agttgaaaaa
    72481 ctaaaaaact tcgttacaat taaatagaat gattcaaaat cgactggcac gagcaataaa
    72541 tggacgctaa tggatactaa gaataatcca attctaagag cttggcagga cgaggaggta
    72601 gtatttttgg ataggaaata gcttgattgt tcgcaaagaa acattaaaaa aaccaatgct
    72661 gaatgtctat ctccaaaata aaatatctgg gattcatatt atgaatacgg ctgtcagcgg
    72721 caataacagt caagctctac gagaacgctt tgccaaagat gtacttagct atacagcaga
    72781 taaagtattt attctcattg ggacgaatga tctagcagag cataaacaac tttctaaaga
    72841 aacgtaccaa aaaatctgca gtggatgata gttcagctga aaaaagacta tagttctgat
    72901 catgctttct ttatcacgcc accagcagtc gatgagacaa aacaaaaata tcggaataac
    72961 aagttgatca aagaatatat ctctgttctc acagaagtag caaaacgcca tgattgtgaa
    73021 gtatcgattt ttatacggaa tttcttgaac aaatggattc gaagaatttt tataatgggc
    73081 taatggatga cggacttcat ttcggcagtg aagggtagga tcttttgtca gacaggatga
    73141 ttcgttatat ttaaagaata ttttgtttaa gcaatgagta ctttttaatc aacttgaaaa
    73201 aacggaggaa tataaaacat gttagaaaaa tcttcaaaag catgcgatac gaataaagaa
    73261 aaagttactg ataagtcaca taatgaacgc attccttggg accagtactt tatggctcag
    73321 gcagtactgt tatctttacg tagtacgtgt acacgtttag aagtaggagc gacactcgtt
    73381 cgagaaaaaa ggatcattgc tggcggctat aatggagcag tcagtggaga tgtccactgc
    73441 atagatgaag ggtgttacgt cgtggatgga cactgcttgc gaacgatcca tgcagagatg
    73501 aatgcactcc tacagtgtgc aaaacttggt atcccgacgg aaggttcaga aatctatgtc
    73561 acacattttc catgtttggc ctgtacaaag gctttgttgc aagctggtgt gaaagggatc
    73621 aattatctcc atgactatcg aaatgatcct tatgcccaag cgttgatcga acaattaggt
    73681 gtatctgtac accaagttgc tttagactca cagcattttt caaaacttca aaatgaatta
    73741 acaggaaaac atgtctagcg ttttagaaag gcatgttcga aatcggttga tttttccggc
    73801 aattacgtcg atgattatct gtttgttgta ttacactgac aattggggtt atcgtttgct
    73861 tcttgccgga atttttttat ggttttcgct aaaagaagga cgttttatcg cgtacttgtg
    73921 tattttgtgt agcataacgg tatccatttc ttataggcta caagagtatt catttttaga
    73981 ggaaaaaaca acatctgcgc aaattactgt atttacagat acgattcgtg caaatggtaa
    74041 ttttttgaca atggaaggaa ggttatcaga gaataaacaa aagctccagc tttcttatca
    74101 agtaaacaca gaagcagaat tgaaaacatg gcttaacttc cgaggaaagg gaatagaatt
    74161 gactgctgaa ggcgagttca tctctccagc aaaaaagcgg aatctttata attttgacca
    74221 agaagcttac ttcaggaacc atagaatttc tggacggttc caaattcaaa agattgaaca
    74281 gatcaccatg caaaatcact ggcgaaactg gcttcagaga aaaagaggat cttttatctc
    74341 ctttgtccaa tctgaatttc cgcaaaaaac aagtgtatat attaattctt tattatgggg
    74401 ctataaagat gcaggattca gagaaccaga agaaattttt agggagagtg gtttgctgca
    74461 tctgttcagt ctatcgggtc tacacatcca attttattta gggtggctgt attatttatt
    74521 tcgacgtatc ggatggccac tacacgtcag tattattccg ttgagtctac taactatctg
    74581 ttatgtttct attgcaggaa gttctatttc tgttttgcgt gctagtttgc tgtttttatt
    74641 gcgattgcta gtcaaattgc tgaataagca ttattccacc atggacttat ttgcagttgt
    74701 cttttggctt cttttactat ttgatcctcg atttcttttt catgcgggtg gacagcttag
    74761 ttttttcatg agttttttgt ttatcctgat ttcttttgat ggagattggt tgaaaaaaac
    74821 ggcctctcac gtcctgttta ctatgatcac gatgccgttg attgtatggc atttttatga
    74881 gtggcctatt ttaggtagtc tttttacatt actgattgca ccggtattca agtttctttt
    74941 tcttcctggt gtgttatttc tcacactctt caatcgctgg ctgccggaaa ctttactttt
    75001 gctgatagaa gagggattaa tcttatttga gcgaataatc gatttttctt cagggtcgtc
    75061 tctcattata ggacgtttgc cccttgtgct atctgggatg tatattttcg gattactgat
    75121 ttgtatggat aggttgaaag aacaacctgt gaaacacttg ttttatgttg gtcttttttc
    75181 ttttattttt ttgactttgc catttttacg cttttcttca acgatcgctt ttatcgatgt
    75241 cggtcaagga gacagtatct ttttgcaaag tccttttcga aaagaaacga ttttgatcga
    75301 tactggaggt cagttacact ttgaaagaga aaagtgggcg caaggtatga tacgtcctcc
    75361 tgctgaaaga aatatcgttc catatctaaa aggtcagggg ataagtgtaa tcgataaact
    75421 catcttgaca cacgacgata cggatcatgt gggagaatta cccacgttat ctcagcattt
    75481 tactatcaaa aaaatttata tcggttgggg cgctggacga aatgagccat tacaaaggca
    75541 tttatctgaa gctcaaaaaa atggtactga gatcattgaa atcaaacatg gggacaggat
    75601 cagcggttat tatgatcttt atgtattgac tccttttgaa aagggagagg gaagaaatga
    75661 agattcgatt ggtctatgga tggaatataa caataggcgc tttttatttt taggagattt
    75721 gaatcaagca atggaaaaac aattgctttt gatttatcct aatctgaaag ccgatgtagt
    75781 caaattaggt catcacggta gccgtacgtc aagtaacaca gattttcttt cccatattga
    75841 agcaaaacat gggattatat cctgcggcgt aaataatcgt tatggtcacc cgcacagcga
    75901 agtattggag acactagaag aaaatcagat aaaaacatgg cgaacggatc aacaaggaat
    75961 gatcacctat cgctggcatc cagtttttca tcctcatggg ctagtggaaa caatgataga
    76021 ctagtaattc tcatctgctc atggtaggat aatttttgaa agtggtgagt aatttgagta
    76081 tacaagcaga gttacaaaaa atacgtaaag aaaaacttgc atcttgttac ttgattttag
    76141 gaacagaaaa gtttctccaa gatcaagttc ggacagaaat tgtcaaaaaa atccaattag
    76201 agggcgaaga tgacttaaat tttttaagtt ttgacatgga aaatgcaagt ttagatgagg
    76261 tagtagctga agcagagacg ttgccgtttt tcggagatca acggttagta ttcgtagaga
    76321 atccttattt tttaacaggt gaaaaagtaa acaatggaat cgaacaaaat actgatttat
    76381 tgacggctta tttgaaagaa ccattagaat cgactgtatt agtattcttt gcgccatatg
    76441 aaaagttgga tgaacggaaa aaagtaacga aacaattgaa aaaaacagca attatcatca
    76501 atgtccaaca gttgaatgaa aaagaagtac gtcaatatct gatgaatacc ttagagaatt
    76561 cctcaatcaa aatggaacga agtgcaattg atttattctt gaggttgacc gacttagatt
    76621 tatccaaaat gatgagagag ttacagaaat tggtccttta tggacaaaat caacagcaaa
    76681 tcacggtaag agaagtagaa gaattagttc ctaaaacgtt agaacataat atttttgaca
    76741 tgacccaata tattttaaaa gggaagacgg aacaagcttt gcgtttatac gaagatttag
    76801 ttatgcaagg ggaagaaaca atcaaaatta atgcgatttt gctttctcaa ttacgtttat
    76861 ttttacagac aaaatttctt gtaaagattg gttatcagca ggcaaatatt gctgaaacat
    76921 tgaaaatcca tccatatcgt gtaaaacttg ccatgcagga agtaaaaaaa ttcgatgaac
    76981 atttgctggt acagttgttt gatcaattag ttgagatgga ttatcaaatc aaaacgggac
    77041 aaatcgaaaa agaattaagt tttcaactat tcgttttgcg cacagggcag ttgttaagaa
    77101 atcgataaga agaaagacga gggcaaagca tgcgggaagg tcgtttgttt ttgtcctttt
    77161 tttgacaaat aaaaaaatcg gccgaagccg attcttttat ttagcgattt ttttgcttaa
    77221 acgagatttg tcgcggttag ctttgttttt gtggattaga ccttttgatt cagccatatc
    77281 aatagcttta acagcttctt tatataacgc atctacgttg tcagcacctg aagctacagc
    77341 atcttcaaat tttttgattg cagtacgcat agcgcttgtt tgagatgaat ttttcacatt
    77401 agcgttttcg cttgtacgaa cacgtttgat tgcagattca atgtttggca ttagtttcac
    77461 ctccgataac ttaagttctt acgcgtgtta ccgcataatt caacattcat tattatacct
    77521 aaaggcagcg tgctttgcaa tatgaacatg acaagttttt ttccttaaca gtcttttatt
    77581 aggatttttt ttcagtaaaa acggatagta caagctgaaa aaaatcaaaa agacgctatt
    77641 gtgggaggca atagcgtcaa accaaactag atttttattt atctatttaa acaaagtttg
    77701 aatgggaatg tttggtacta agttattata taacaaagag aaccgtttgc aaagtttttt
    77761 ttgaaaaggg atacgattcc ctgttgttct ttgtttttct attaagaaac tgcagttatt
    77821 ttataaccag tatcctttga tcagtaaagg ctattctggt gtaccgatta ttttaatgta
    77881 ttcatcgata ttttttatca acattttttt tatgaccaac tggttccgac ggttccattt
    77941 gccacttact tccagcacat atttatttag gggcaaacgt aagatatatt ttgccaaata
    78001 gcgatcttta agcaggccat tgatcacacg gtcctcaagc tgcaagttga tcccagtcag
    78061 catcattggc tgttcttgta aaacgtacgt atcaattaaa agaccttcaa aagtttccac
    78121 aagcgtcttc ctttcttttc aaagtaacct gaaaacaggc actaattctt ccattttgat
    78181 attcgttgaa gtggatgcga tcattctttc ttttgtccac tgtaatctag tataacaaaa
    78241 aagagcagaa gagccaaaaa gaaaaaagag catcgaattc tataggaagt ctcattggca
    78301 cggcctaaat tattacaaaa cattgattaa aaaaagcccc tgcatcgcag gagcatcccc
    78361 tatatcgttt taataggtca atcaaaaagc agtatcaaac acattttgta tcaaatcagg
    78421 aaataataga taagctaagc ccagaatcaa aagtaccgct agaagaaacc agccgattgt
    78481 tcctatgaaa tactttaatt tttcgaacat tgtatcacct cgttgtcagt atatcacgga
    78541 tcagtaaatt gggggtgtta agaatctcct aaaatagaca aaatttgttc tattttccta
    78601 aagcaaatac ttgtaagata cagtaagtga taccagaagc aatgaccggt ccggcggcga
    78661 ttcctcttaa gaagacaaca cctaaaatcg tgccgaaaac aagtgcaaca gtgatttgtg
    78721 gatcagaagc aatcaatcct acacccttgg aagataagac agccactagc ccaccgcaaa
    78781 aaacagctac ccagccaata ggggatttaa aggcttctaa tagatcacgt agaccgattt
    78841 ctcctgttgc gatagggatt agaatagccg ctgtgatgat cgtcacccca atattgattc
    78901 ctttgccttg aagccaaggg aaaaaggaac gggaggctgg tatgagcttc ataatcaaaa
    78961 caaaagctgc cgctatcatc agactttgat ttttagctaa aattgctaca agtaaaataa
    79021 ttcctaaaaa aagccaactt tccatgaaat atattcctca ctttcttcga aacaatcata
    79081 acatgttttt agaaagttac aaaagtagga attcatcctt caagacctat tcataagctg
    79141 tttttttcat aaaataaaca aaatcataaa aaaacacttt gcttatttga cagaagacag
    79201 ccgcgctttg tatagtggac aagacttaca tttttttgaa gaatcagaaa gatcaggtaa
    79261 aggtcttagt aaacgcaagc cattcagaat aaccaatatt gtgcttcctt cgtgtcctac
    79321 aactccaaga ggtagattta aaaattgcag gaagttagaa gcaatcaaca agagaataac
    79381 aagaatcgaa aaaataatat tttgtttgat gatccgccgc atttttaaag atagccgatg
    79441 actgtagaca aatttattta agtcgctttt cattaagacg atgtctgcga tatccatagc
    79501 ggcatctgtc ccttgcccca tagcgacacc taaagaagcg gctgctaaag caggcgcatc
    79561 attgactcca tcaccaacca tcgcatttac ttggtaaagt tcattctctt ttttgactag
    79621 ttgtgttttt tcttccggag tacagcttgc atagtaatgt tcaatcccta cttgttcagc
    79681 aatggccttt gctgtgccag aatggtcgcc ggttatcata cttgtatgga tgttttggga
    79741 tttaaagtag gaaattactt gttgggtatt tgcttttgga atatctagca gacctaaaac
    79801 agcaattaac tgctgatctt tagaaagata aatcacggtt ttgccttgat tttccaaacg
    79861 ttcgatcatt tccgcaatct ctttgctgat tatcatcgtc tcaggatcat atgcgtgctt
    79921 gccaactttc cattgcgcac ctaaataggt agtttgtaat ccaaaaccag taatttcttc
    79981 tattggtaag tattggattt cttcaggtat tgtgcaatcg aaatattgca aaatcgcatg
    80041 agcaagtgga tgcgtcgttt tttgttccat agcaacaaga agttgttgtg ccaaatcctt
    80101 ttcatcaaag aaaaagctgt cagttacaac tggttttcct ttagtgagcg ttcctgtttt
    80161 atcaaaggca atcgctttta attcagaaag cttttctaaa tggataccgc ctttgattaa
    80221 aatcccattt tttgcagaat ttgaaagagc agctagagaa gctggtgtag cagatgcgac
    80281 aagcgcacaa ggagaagcca caactaataa taccattcct cgataaaagc tttcatccca
    80341 actccaacct aggaaaaagt gggggagaag gatcattaga ggaacagcaa gcaaaatcaa
    80401 ttttacataa ttattttcga ttcgttcgat aaatgaagct gtttgagttg gcgtattctg
    80461 cgcttcttct actaattgga taatcttagc aaagacagtt tcggaactcg tttttgttac
    80521 tcgaatcgtg atgggatttc cttgattcag cgttcctgca tacatctcat cgcctaaata
    80581 tttttctaca ggcacagatt caccgcttaa tgcagactca tcgatcgttg cctgtgtgct
    80641 ttctaaaaag ccatctatcg gaacagctgc tcctttagcg acaaatacca agttatccac
    80701 ctttagttga gaaacatcta cttcttgcat ttgtccattt tcttttaata attgggcttt
    80761 ttgtggttga agattcatca aagcagtaat ttctttttga cttttattag ttgtgtattc
    80821 ctctaatgca ccgcttaaac aaaagatgaa tgtcaacatt gctccttcaa agtaattacc
    80881 gatcaaacag gcaccaatag cagctaaagc catcaataaa tccacattca gtgtccgatc
    80941 attccacaat tctttcaagc cttccgatgt ttgtttatat ccaccgatga taatggcact
    81001 gatataaaaa agtggggtga tggtaagaga gaatcggtcg aagataaaac cgaagatcat
    81061 aaataaaagg cagccgattg ttgctgcaaa agcttttttc tgattagtca tcgttattag
    81121 acctccttgt caaaacaagg atagcataat aaaaatgtaa ttgataataa taacagctaa
    81181 atgagaacga taatggttat catttggtga ttaagcttga tgttgttttt attatttata
    81241 atgattatta ttaaagaaaa taaaaaacca gaacatttct caaataagag
//
LOCUS       DACDTO010000004        73460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDTO010000004 DACDTO010000000
VERSION     DACDTO010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.004"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            221..535
                     /locus_tag="IWK70_000250"
     CDS             221..535
                     /locus_tag="IWK70_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9612827.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            556..933
                     /locus_tag="IWK70_000251"
     CDS             556..933
                     /locus_tag="IWK70_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9612828.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            943..1716
                     /locus_tag="IWK70_000252"
     CDS             943..1716
                     /locus_tag="IWK70_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612829.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1738..3141
                     /locus_tag="IWK70_000253"
     CDS             1738..3141
                     /locus_tag="IWK70_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9612830.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3323..4507
                     /locus_tag="IWK70_000254"
     CDS             3323..4507
                     /locus_tag="IWK70_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9612831.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4504..4794
                     /locus_tag="IWK70_000255"
     CDS             4504..4794
                     /locus_tag="IWK70_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612832.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4791..5012
                     /locus_tag="IWK70_000256"
     CDS             4791..5012
                     /locus_tag="IWK70_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612833.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5054..5833
                     /locus_tag="IWK70_000257"
     CDS             5054..5833
                     /locus_tag="IWK70_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9612834.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5900..6394
                     /locus_tag="IWK70_000258"
     CDS             5900..6394
                     /locus_tag="IWK70_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9612835.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6450..7088
                     /locus_tag="IWK70_000259"
     CDS             6450..7088
                     /locus_tag="IWK70_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612836.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7160..7555
                     /locus_tag="IWK70_000260"
     CDS             7160..7555
                     /locus_tag="IWK70_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9612837.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7539..9992
                     /locus_tag="IWK70_000261"
     CDS             7539..9992
                     /locus_tag="IWK70_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9612838.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            9989..12016
                     /locus_tag="IWK70_000262"
     CDS             9989..12016
                     /locus_tag="IWK70_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612839.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12013..13035
                     /locus_tag="IWK70_000263"
     CDS             12013..13035
                     /locus_tag="IWK70_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9612840.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13052..13981
                     /locus_tag="IWK70_000264"
     CDS             13052..13981
                     /locus_tag="IWK70_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9612841.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14226..14342
                     /locus_tag="IWK70_000265"
     CDS             14226..14342
                     /locus_tag="IWK70_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9612842.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14358..15161
                     /locus_tag="IWK70_000266"
     CDS             14358..15161
                     /locus_tag="IWK70_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9612843.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15336..15632
                     /locus_tag="IWK70_000267"
     CDS             15336..15632
                     /locus_tag="IWK70_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612844.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15639..17591
                     /locus_tag="IWK70_000268"
     CDS             15639..17591
                     /locus_tag="IWK70_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9612845.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(17663..18160)
                     /gene="dfrG"
                     /locus_tag="IWK70_000269"
     CDS             complement(17663..18160)
                     /gene="dfrG"
                     /locus_tag="IWK70_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9612846.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18522..19562
                     /locus_tag="IWK70_000270"
     CDS             18522..19562
                     /locus_tag="IWK70_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9612847.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            19660..19848
                     /locus_tag="IWK70_000271"
     CDS             19660..19848
                     /locus_tag="IWK70_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9612848.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(19906..20259)
                     /locus_tag="IWK70_000272"
     CDS             complement(19906..20259)
                     /locus_tag="IWK70_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9612849.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            20790..21212
                     /locus_tag="IWK70_000273"
     CDS             20790..21212
                     /locus_tag="IWK70_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9612850.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            21209..21439
                     /locus_tag="IWK70_000274"
     CDS             21209..21439
                     /locus_tag="IWK70_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9612851.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            21937..22137
                     /locus_tag="IWK70_000275"
     CDS             21937..22137
                     /locus_tag="IWK70_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9612852.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            22164..23357
                     /locus_tag="IWK70_000276"
     CDS             22164..23357
                     /locus_tag="IWK70_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9612853.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(23425..24990)
                     /gene="guaA"
                     /locus_tag="IWK70_000277"
     CDS             complement(23425..24990)
                     /gene="guaA"
                     /locus_tag="IWK70_000277"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9612854.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(25049..25972)
                     /locus_tag="IWK70_000278"
     CDS             complement(25049..25972)
                     /locus_tag="IWK70_000278"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9612855.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            26168..26773
                     /locus_tag="IWK70_000279"
     CDS             26168..26773
                     /locus_tag="IWK70_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9612856.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            27081..28097
                     /locus_tag="IWK70_000280"
     CDS             27081..28097
                     /locus_tag="IWK70_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9612857.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            28238..29539
                     /locus_tag="IWK70_000281"
     CDS             28238..29539
                     /locus_tag="IWK70_000281"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9612858.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            29585..30247
                     /gene="deoC"
                     /locus_tag="IWK70_000282"
     CDS             29585..30247
                     /gene="deoC"
                     /locus_tag="IWK70_000282"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9612859.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            30268..30666
                     /locus_tag="IWK70_000283"
     CDS             30268..30666
                     /locus_tag="IWK70_000283"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9612860.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            30732..31814
                     /locus_tag="IWK70_000284"
     CDS             30732..31814
                     /locus_tag="IWK70_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9612861.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            32026..33588
                     /locus_tag="IWK70_000285"
     CDS             32026..33588
                     /locus_tag="IWK70_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9612862.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            33581..34714
                     /locus_tag="IWK70_000286"
     CDS             33581..34714
                     /locus_tag="IWK70_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9612863.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            34714..35670
                     /locus_tag="IWK70_000287"
     CDS             34714..35670
                     /locus_tag="IWK70_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9612864.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            35769..36935
                     /gene="deoB"
                     /locus_tag="IWK70_000288"
     CDS             35769..36935
                     /gene="deoB"
                     /locus_tag="IWK70_000288"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9612865.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            36959..37777
                     /locus_tag="IWK70_000289"
     CDS             36959..37777
                     /locus_tag="IWK70_000289"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9612866.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            37794..38501
                     /gene="deoD"
                     /locus_tag="IWK70_000290"
     CDS             37794..38501
                     /gene="deoD"
                     /locus_tag="IWK70_000290"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9612867.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(38799..39485)
                     /gene="gpmA"
                     /locus_tag="IWK70_000291"
     CDS             complement(38799..39485)
                     /gene="gpmA"
                     /locus_tag="IWK70_000291"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9612868.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(39657..40238)
                     /locus_tag="IWK70_000292"
     CDS             complement(39657..40238)
                     /locus_tag="IWK70_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9612869.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(40371..41054)
                     /gene="rpiA"
                     /locus_tag="IWK70_000293"
     CDS             complement(40371..41054)
                     /gene="rpiA"
                     /locus_tag="IWK70_000293"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9612870.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            41373..41786
                     /gene="rpsL"
                     /locus_tag="IWK70_000294"
     CDS             41373..41786
                     /gene="rpsL"
                     /locus_tag="IWK70_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9612871.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            41870..42340
                     /gene="rpsG"
                     /locus_tag="IWK70_000295"
     CDS             41870..42340
                     /gene="rpsG"
                     /locus_tag="IWK70_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9612872.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            42434..44521
                     /gene="fusA"
                     /locus_tag="IWK70_000296"
     CDS             42434..44521
                     /gene="fusA"
                     /locus_tag="IWK70_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9612873.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            44693..45880
                     /gene="tuf"
                     /locus_tag="IWK70_000297"
     CDS             44693..45880
                     /gene="tuf"
                     /locus_tag="IWK70_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9612874.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            46287..46595
                     /gene="rpsJ"
                     /locus_tag="IWK70_000298"
     CDS             46287..46595
                     /gene="rpsJ"
                     /locus_tag="IWK70_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9612875.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            46626..47255
                     /gene="rplC"
                     /locus_tag="IWK70_000299"
     CDS             46626..47255
                     /gene="rplC"
                     /locus_tag="IWK70_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9612876.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            47283..47906
                     /gene="rplD"
                     /locus_tag="IWK70_000300"
     CDS             47283..47906
                     /gene="rplD"
                     /locus_tag="IWK70_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9612877.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            47906..48196
                     /gene="rplW"
                     /locus_tag="IWK70_000301"
     CDS             47906..48196
                     /gene="rplW"
                     /locus_tag="IWK70_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9612878.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            48235..49068
                     /gene="rplB"
                     /locus_tag="IWK70_000302"
     CDS             48235..49068
                     /gene="rplB"
                     /locus_tag="IWK70_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9612879.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            49111..49389
                     /gene="rpsS"
                     /locus_tag="IWK70_000303"
     CDS             49111..49389
                     /gene="rpsS"
                     /locus_tag="IWK70_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9612880.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            49412..49759
                     /gene="rplV"
                     /locus_tag="IWK70_000304"
     CDS             49412..49759
                     /gene="rplV"
                     /locus_tag="IWK70_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9612881.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            49773..50429
                     /gene="rpsC"
                     /locus_tag="IWK70_000305"
     CDS             49773..50429
                     /gene="rpsC"
                     /locus_tag="IWK70_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9612882.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            50432..50866
                     /gene="rplP"
                     /locus_tag="IWK70_000306"
     CDS             50432..50866
                     /gene="rplP"
                     /locus_tag="IWK70_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9612883.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            50856..51044
                     /gene="rpmC"
                     /locus_tag="IWK70_000307"
     CDS             50856..51044
                     /gene="rpmC"
                     /locus_tag="IWK70_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9612884.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            51069..51335
                     /gene="rpsQ"
                     /locus_tag="IWK70_000308"
     CDS             51069..51335
                     /gene="rpsQ"
                     /locus_tag="IWK70_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9612885.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            51393..51761
                     /gene="rplN"
                     /locus_tag="IWK70_000309"
     CDS             51393..51761
                     /gene="rplN"
                     /locus_tag="IWK70_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9612886.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            51798..52106
                     /gene="rplX"
                     /locus_tag="IWK70_000310"
     CDS             51798..52106
                     /gene="rplX"
                     /locus_tag="IWK70_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9612887.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            52134..52673
                     /gene="rplE"
                     /locus_tag="IWK70_000311"
     CDS             52134..52673
                     /gene="rplE"
                     /locus_tag="IWK70_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9612888.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            52692..52877
                     /locus_tag="IWK70_000312"
     CDS             52692..52877
                     /locus_tag="IWK70_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9612889.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            52913..53311
                     /gene="rpsH"
                     /locus_tag="IWK70_000313"
     CDS             52913..53311
                     /gene="rpsH"
                     /locus_tag="IWK70_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9612890.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            53343..53879
                     /gene="rplF"
                     /locus_tag="IWK70_000314"
     CDS             53343..53879
                     /gene="rplF"
                     /locus_tag="IWK70_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9612891.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            54040..54396
                     /gene="rplR"
                     /locus_tag="IWK70_000315"
     CDS             54040..54396
                     /gene="rplR"
                     /locus_tag="IWK70_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9612892.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            54417..54917
                     /gene="rpsE"
                     /locus_tag="IWK70_000316"
     CDS             54417..54917
                     /gene="rpsE"
                     /locus_tag="IWK70_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9612893.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            54932..55111
                     /gene="rpmD"
                     /locus_tag="IWK70_000317"
     CDS             54932..55111
                     /gene="rpmD"
                     /locus_tag="IWK70_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9612894.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            55155..55595
                     /gene="rplO"
                     /locus_tag="IWK70_000318"
     CDS             55155..55595
                     /gene="rplO"
                     /locus_tag="IWK70_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9612895.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            55595..56890
                     /gene="secY"
                     /locus_tag="IWK70_000319"
     CDS             55595..56890
                     /gene="secY"
                     /locus_tag="IWK70_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9612896.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            56952..57599
                     /locus_tag="IWK70_000320"
     CDS             56952..57599
                     /locus_tag="IWK70_000320"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9612897.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            57790..58008
                     /gene="infA"
                     /locus_tag="IWK70_000321"
     CDS             57790..58008
                     /gene="infA"
                     /locus_tag="IWK70_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9612898.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            58041..58157
                     /gene="rpmJ"
                     /locus_tag="IWK70_000322"
     CDS             58041..58157
                     /gene="rpmJ"
                     /locus_tag="IWK70_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9612899.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            58175..58540
                     /gene="rpsM"
                     /locus_tag="IWK70_000323"
     CDS             58175..58540
                     /gene="rpsM"
                     /locus_tag="IWK70_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9612900.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            58568..58957
                     /gene="rpsK"
                     /locus_tag="IWK70_000324"
     CDS             58568..58957
                     /gene="rpsK"
                     /locus_tag="IWK70_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9612901.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            59037..59975
                     /locus_tag="IWK70_000325"
     CDS             59037..59975
                     /locus_tag="IWK70_000325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9612902.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            60003..60383
                     /gene="rplQ"
                     /locus_tag="IWK70_000326"
     CDS             60003..60383
                     /gene="rplQ"
                     /locus_tag="IWK70_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9612903.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            60655..61494
                     /locus_tag="IWK70_000327"
     CDS             60655..61494
                     /locus_tag="IWK70_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9612904.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            61470..62339
                     /locus_tag="IWK70_000328"
     CDS             61470..62339
                     /locus_tag="IWK70_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9612905.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            62329..63126
                     /locus_tag="IWK70_000329"
     CDS             62329..63126
                     /locus_tag="IWK70_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9612906.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            63329..64075
                     /gene="truA"
                     /locus_tag="IWK70_000330"
     CDS             63329..64075
                     /gene="truA"
                     /locus_tag="IWK70_000330"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9612907.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(64408..67077)
                     /locus_tag="IWK70_000331"
     CDS             complement(64408..67077)
                     /locus_tag="IWK70_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9612908.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(67315..67503)
                     /locus_tag="IWK70_000332"
     CDS             complement(67315..67503)
                     /locus_tag="IWK70_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9612909.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            67773..68135
                     /locus_tag="IWK70_000333"
     CDS             67773..68135
                     /locus_tag="IWK70_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9612910.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            68187..69869
                     /locus_tag="IWK70_000334"
     CDS             68187..69869
                     /locus_tag="IWK70_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9612911.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            69986..72022
                     /gene="recG"
                     /locus_tag="IWK70_000335"
     CDS             69986..72022
                     /gene="recG"
                     /locus_tag="IWK70_000335"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9612912.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            72071..73072
                     /gene="plsX"
                     /locus_tag="IWK70_000336"
     CDS             72071..73072
                     /gene="plsX"
                     /locus_tag="IWK70_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9612913.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            73194..73436
                     /gene="acpP"
                     /locus_tag="IWK70_000337"
     CDS             73194..73436
                     /gene="acpP"
                     /locus_tag="IWK70_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9612914.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT        24145 a        13098 c        15416 g        20801 t
ORIGIN      
        1 gaatttgatt ataatattta aaatacctag gtagtcgttg ttttaaatat agtaggaatc
       61 ggacgctcag tacggagcgt ctattttttt acccaatttt gaaaggaagt gaacttatga
      121 aaataaaaaa tcaagaatca aaaggtcgct ctcccccctt taaaaccatc acacaaataa
      181 gattcattca aacataaaaa cagaaaggat aggtaaaaat atggaactta aatatgtcat
      241 tcccaatatg gaaaaaacat ttggacaatt agaatatgct ggtgaaggga acattgaaca
      301 acgtcgaata aatggcagac caaccattct ctctcgtagt tacaatttat actcaacgat
      361 tcaacgagca gatgatattg ttgtcattct tccctccgaa gctggcgaga aacattttga
      421 atccgacgaa aaagtcaaac tcatcaaccc acatattacc gcagaaggtt ataaaattgg
      481 caatcgagga tttaccaatt acattatgca cgcagacgat atggtgaaag cataagaaag
      541 gaaaggtgaa aaaatatgag attagcagaa ggtattgtga ttgaaaaaga ccagacattt
      601 ggcacattga aattttccgc cttgcgtcgt gaggttcgta tccagaatga agatggtacg
      661 gtttccgaag aaattaaaga acgtacctat gacttaaaat caaaaggaca aggacgcatg
      721 atacaagtca gtatccccgc ttctgttcct ttgaaagaat ttgactacaa cgcagaagtg
      781 gaattaatca atcctgtggc agataccgta gcgacggcta cttttcgagg ggcagacgtt
      841 gattggtacg tgaaagcaga cgatcttgta ttaaaaggac agacaaaacc tcatcagaaa
      901 tataaaaaag aagcgtccga aagtgaaaaa taggtgttgc ttatgacaaa cacaccattg
      961 attttgaaag aaattccgaa ggatgaagcc atttctttta ttcggcaata ccattattca
     1021 aagatactcc ctagattgtg taaatacttt ctagggattt tttcagaaga aaaattatta
     1081 ggcgttgtgg aattaggctg gggtacgcaa cccctacaaa ccatacgcaa actctttccc
     1141 gacagttccc ttcaaaccac agattattta gaaattggaa agatgtgttt tcttccagaa
     1201 atgaatcaaa ccaattattt tggcagtcaa gctctttcag ctctaattaa gtggttgaaa
     1261 gaacatacgg actgtcattt tttatataca ctggcagatg gaattgaggg aaaatgtggc
     1321 tatgtctatc aagcgtccaa cttcttttac tgcgggtatt tcaagacaag tgtctatcgt
     1381 gataagcagt catgggaaaa gatacaccct cgaagtgctc gccttttatt agaggaaaat
     1441 gctcgctttg aacaggtaga gaaaaagcat tggctttccc aggcattttg cgaatacaaa
     1501 gggattgaga aaatcaatgg acgcatgttt cgctatctct atccgttgac aaaagaagcg
     1561 aaaaaactgt taggacacac tctctatcga cgtcattatt atccgaaaga aaaaaattta
     1621 cgctttgaaa agcgaatcgc ttatcagaaa tatgaagcca tttctcaacc aacttttgat
     1681 aaacaggctc gtatctacaa tacacaatta ttttgatagg tggaaaggag gatttttatg
     1741 ccattgcttc atgacagagg agagcgaatc aaagccaacg ataagcactt ggtctatcat
     1801 tttgtactaa tttcattgct ggtagttttt ctagtcgtcc tactcccttt tcattggaga
     1861 gcgttacaat cggtgaactg gcaagcagtt cagttagata catttttcta tcaacttcat
     1921 acgccatact tttggagcag tattctcact gctctttttg tatgcggact tatcagctgg
     1981 ctttattatc actatcgtat agattctata aaacagttag agcaccgtca aaagttagct
     2041 cgtatgattt tagaaaatca ttggtatgaa gccgaagaaa ctcaatcaga gggattcttt
     2101 aaagatttac ccagcaagaa aaccaaacaa cgaatccgcc attttcctaa aatatattat
     2161 cgaatgaaag atggactaat ccatattcat gtagaaatca ccttagggaa atatcaagat
     2221 cagcttttgc acctagaaaa caaactagaa acaggattgt attgtgaatt agtggaaaaa
     2281 acgttgaaag attcttatgt agagtatgtc ctactttatg atactattgg caatcgaatt
     2341 ggcattgaag acgtggtagc tcaagatgga cgtgtcaaat tgatggaatc ggtctattgg
     2401 gagttcgatt ctctccctca catgttaatt gctgggggca ctggtagtgg aaaatcctat
     2461 tttattctca cattgattga atctctcctg catacaaatg caaagcttta tattcttgac
     2521 cctaaaaatg ctgatttggc agacttaggg gcggtcatga ataatgtcta ttaccgtaaa
     2581 gaagatatgt tggcatgtat caatcaattt tacgaagata tgattgatcg cagtgaaacc
     2641 atgaaacaac accctaacta taaaacagga gaaaattatg cttatcttgg tttgtctgcc
     2701 aattttctta tttttgatga atatgtggca atgatggata tgctaggtcg tgaaagtacc
     2761 accgttattc ataaactcaa acaaattgta atgttaggac gacaagcagg cttcttttta
     2821 attcttgcct gtcaacgtcc agacgcaaaa tattttagtg atgggattcg ggacaacttt
     2881 aacttccgtg tggctttggg tcgtatgtct gaaatgggct ttggtatgat gttcggaagc
     2941 gatacacaaa aacaattttt cttaaaaccc atcaagggaa gaggttacgt ggatacagga
     3001 aaaagtgtga ttagtgagtt ttatacaccc cttgtaccaa aaaggtacga tttcttagga
     3061 gaaataggaa aagtcattca aaaaaagcaa tctgagcctg taccgcacga agtgaaaggt
     3121 acaggttcag acttgctttg attggtgtgg cgttagccac gccaatcctt tagccccctc
     3181 tatctaacag gggggcacaa atacaactga aaacaggcaa ttctcatcaa ccaatgttga
     3241 tggtacaaag gattgcggaa aatatgaaag gtgtaaactc aagggtcata gtttacatct
     3301 tttttagatt ggaggtttgc gaatggaaaa cgaaaaaatt tggattaagg aattgaaaga
     3361 aaagcgactg gcttatggcg tttctcaaaa taaactggcg gttgcttctc atattaccag
     3421 accttaccta tcggatattg aaacaggcaa ggcagtccct actcaacagg tgaaagaaga
     3481 tttactgaac gctttggaac gcttcaatcc cgataaccca ttggagatgt tattcgacta
     3541 tgtacggatt cgattcccta ccaatgatgt ggctcaaatt atcggtgagg ttttacgttt
     3601 gaatatggat tatatgctac acgaagactt tggctattat tcttatcccg aacactatcg
     3661 tttcggtgat attgttgtcc tagtctctca tgatgtaagt aaaggtgttt tattagaact
     3721 gaaaggaaaa ggctgtcggc aatttgaaaa ctttctattg gctcaacatc ggagctggta
     3781 tgatttcttt caagattgca tggaacataa aggtattttc aaacgcttgg atttagctat
     3841 caacgataaa acagggattt tgaatattcc agatttggca aagaaatgta aacaggaaga
     3901 atgcatttcc gtctttcgaa gctttaaaaa ttatcgcagt ggtgaattgg ttcaccgtga
     3961 tgaaaaacca tacatgggaa acacacttta tattggctca ttaaaaagtg aagtctattt
     4021 ctgtatatat gagaaagatt atgagcaata cgtgaaaaat gatgttccct tagaagacgc
     4081 agaagtaaag aatcggtttg aaattcggtt aaaaaatgac cgagccaccc aagccatgaa
     4141 agatttattg gcacatcaac aggcggaaaa aacagccttt gaaattatca atcgctatat
     4201 ccgctttgca gataaagacg acactaaacg tcggagtgat tggaaaacta atgaacgttg
     4261 ggaatggttt atcggtaaaa atcgtggagc attacgccta accacacagc cagagcctta
     4321 ttcttttgaa cgaacattga actggttaca tcatcaagtc gcccctacat taaaaatcgc
     4381 aagcatttta gatgttctca acggtacaac cattatctcc accatgattc aggaagccaa
     4441 actgacagaa aaacatgaga aattaattga acaacaacat ctagccatgg aggatttgat
     4501 tacatgagcc ttttaacaaa agaattaaag aagttagggt ttcagtgtgg cattgagttt
     4561 caagcgtata tacaaaatac agggaaatat acgtctctca ttattgaggg aaaacggcaa
     4621 gctggtgata ccatctatac ctatgatttt tacaaggtga gattttataa taattatacc
     4681 aatcgagtaa ccgtctatgg agagcattta accccttttc aattattaag gcgtgtaaaa
     4741 agctatatct attatcgaga aaaatatctt aaggaaagga ggacaatcac atgaattttg
     4801 ggcagaattt gtacaactgg ttcttggata atgcacagtc attggtattg ttagcgattg
     4861 ttgtcattgg actgttttta ggttttaaac gagaattttc aaaactcatt ggcttcttga
     4921 ttattgctct aatcgccgtc ggacttgtgt tcaatgcagc tggcgtgaaa gatgtgttat
     4981 taaacttatt taaccgaatc attggtgctt aacagatatt tttcatgata aaataggaag
     5041 tatatatttt gttatgaaag gatttatgaa tatgtttaca aatgatcaat ttaaatctct
     5101 tttaattaaa acattagaaa gaaagagctc ttcaaattat tatgaaggag gtaacgagat
     5161 tgtttctaaa atgaaaatat cagaggtaac tttagatgag actgacgatt ttacttatta
     5221 taaaggatat aaaaagggct ggaatacgct ttgcacatat cttaaaataa gagttccttt
     5281 tgatgattta gacttttttg aaagtcataa agatttgatt acccagacag caagctccat
     5341 atatgataaa caaggtgata atgtattagt ggatactatt ttagtaccac ttccagagaa
     5401 ttacgaagta attaacttta gtcagttgaa aattagtgat gtggtttcac aagctattga
     5461 agacgctgag tcttttatga gtaatggtga atatcaacgt gcctttgaca gagttcacac
     5521 tgcttttcat ggatatttaa ttgaaatact aaaaaaatat gaaattacag ttcctagaga
     5581 cgaaaatcta tctaaattat atagtaggat acaacaatta attgaaaaag aaattcaacc
     5641 tactgaactt gctgatatag tgaagacaac gataaggtca tcaaatggaa tgattagctc
     5701 tcttaatgaa gcaagaaata ggcattcact tgcacaccct aatacaaata ttattggaaa
     5761 gagagaagca aaactaataa taggtatttc aagtacagtt acagattata ttagcggata
     5821 ccttgataag tagcgtcatt aactgattgc tacttttttt atgactattt ttataaaaag
     5881 gatgtgataa gaattggaaa tgaaagtcta tgtggcaaat ttaggacggt ataatgaagg
     5941 tgaattagtc ggagcatggt ttactccacc aattgatgag gaagaaatgg cagaaagaat
     6001 cggtttaaat gaggactacg aagaatatgc aatacatgat tttgagcttc cttttgatgt
     6061 ggacgaatac acgccgatta gtgaaatcaa ccgtctatgt gaagccattc aagaaatcga
     6121 aggtacacct atttataatg aactcaaaga aattcaaggc atgtggttta gtagcttgga
     6181 agaattactg gaaaacaaag aggatattca ttgttatagt gattgtgatt cgatggaaga
     6241 tgtcgcccgt tattacgtgg aagaaacagg acagcttggt gaagtccctt ccaatttaca
     6301 aaattatatt gactaccaat ctttgggacg tgatatggaa atagagggta attacctagt
     6361 tacctctcat ggggtatttg aatattgtca gtaagtttaa caatccgtgt tataattaaa
     6421 ttaataaata gcacggagag gtataaatta tggtaaatgt atttgaaaat cttaaactat
     6481 taaaactaga catgaaagat aaaggctggg tgatagattc cttttacttt cgttacaaac
     6541 agcaaaatta tattgtactt gttaagctat ttgaaaaaga ggaaaaagtt ccagaatatg
     6601 ctctgttaaa actagaattt ttaaaggaaa acgatttttc agatatgcta gctgtctatg
     6661 ctaattcggt aaaattatat acagacacga aaacaataag agaatatttt ggtatcgaat
     6721 acagtagtaa cttgggagat gttttatttc aatttagcca aactcttgct cgatttattc
     6781 cgacagaagt tagtgagaag aaaaatgaag accaaaaaga agccatgtgt ttctcattaa
     6841 gtcaaagtga ttcagaagat cccagaaaaa aatattgttt ttcagttaga agaaatcctt
     6901 taaagggaaa tggtgagctg ggaaaacgaa gtccttttaa tgacaataaa acgagattgt
     6961 atagacccag cttatatgaa cgattagggt cagataccaa tttaagtttc agatattcaa
     7021 tgaatccaga tgatgaagaa actgatgaag gtattattgc aaaatggaca aaaaataaaa
     7081 ttgaatagtt aaagcattga aagagcaatc acctattttg attgttcttt ttctattttt
     7141 acggaaagga cgtgaagaca tgaaaaaaat aagaagttac accagtattt ggtcagtaga
     7201 aaaagtgatt tatgcgatta atgatttaca actccctttc ccaattacct ttacgcaaat
     7261 gacatggttt atcgtttctt tgtttgtcgt catactcttt ggcagtttac caccactttc
     7321 tctgattgac ggagcactac tcaaatactt agggattccc attgttctca ctgggttcat
     7381 gtgccaaaag acctttgacg gaaagaaacc ctatggattt ctaaagtcta tcattaccta
     7441 tgctctacgc cctaaagtaa cctacgctgg caaacaaata cgattaaaaa aagaaacaat
     7501 gaatgaatcg attacgattg taaggagtga acaactagat ggcatatccg attaaatata
     7561 tggaagataa tctcgtattt aatcatgatg gcgaagtatt cgcttattat gaattaattc
     7621 cctataatta cagtttttta agttccgatg aaaaaattca agttcatgat aatttctgtc
     7681 aattgattgc ccaaaatcgt gatggcaaga ttcatgctct gcaactagca accgaatcaa
     7741 gtattcgagc cacacaggaa caatcgaaaa aagaaatcac tggacgatta aaagagatag
     7801 cttacgaaaa aatagatgaa cagaccgaag cgttaatttc tatgattggt gacaatcaag
     7861 tagactatcg cttttttatt ggttttaaac tctacctcaa tgaacaggaa gtatccgtaa
     7921 aaaatgttgg gaaagaaatt caaaaagcat ttgcggaatt ctttcatggt gtgaatcaca
     7981 aattaatggg cgattttgtt tctatgccta tggcggaaat cgaacgcttt tctaagatgg
     8041 aacagttgtt agaaagtaaa ttatccagac gctttcagat tcgtaaattg gagaaagatg
     8101 attttggtta tattattgaa catctttacg ggcaaacagg tgtccctttt gatgactacg
     8161 actaccattt accgaaacga tacgaagata aaaaagtgtt ggtaaagaaa tacgatattc
     8221 tcaaacccac tcgctgttta atcgaagaaa atcaacgcta tttacggatt gaacaggaag
     8281 ataccactac ttatgtggcg tattttacaa ttaatagtat tgttggtgaa ttggatttcc
     8341 ctaatagtga aattttttat taccagcaac agcagtttac tttccccgtt gataccagta
     8401 tgaatgttga aattgtcgcc aataaaaaag ccttatctac cgttcgcaat aaaaagaaag
     8461 aactgaaaga tttagatagc cacgcatggg aaagcgacaa cgaaaccagt actaatgtgg
     8521 tggacgcttt agaatctgtc gatgaattag aaagtaattt agaccaaacc aaagaatcta
     8581 tgtacaaact aagctatgtg gtacgggtat cagcaaagaa tttggacgag ctgaaacaac
     8641 gttgtgatga agtaaaagat ttttatgatg atctgtctat caaattagta cgaccttttg
     8701 gcgatatgct aggcttgcat aatgaattta ttccctcaag caagcgatat atcaatgact
     8761 atattcaata tgtcacgtcg gattttttgg ctggtcttgg tttcggtgct acacaaatga
     8821 ttggtgaaaa ttctggtatt tacgtcggtt acaatctcga tacaggcaga aatatttatc
     8881 tccaacctag ccttgcaagt caaggaatta aaggctctgt aacaaatgca ttagcgtccg
     8941 cttttattgg ttcattaggt ggcggaaaat cattctctaa caatatgctg gtctattatt
     9001 cagtactctt tggcggacag gcaaccattg ttgacccaaa agctgaacgt ggtgaatgga
     9061 aagaaacctt acctgatatt gcccatgaaa tcaatattgt taatctcacg agtgagaacg
     9121 acaataaagg gttacttgac ccttatgtga ttttaaaact cccgaaagat tcagaaagcc
     9181 ttgccattga tattctcacg ttcttaacag gtatttccag ccgagatagc gagaagtttc
     9241 ctgtcctgcg taaagctatt cggagtgtaa ctcaaagcaa acaacgaggg ttgttattgg
     9301 tgattgatga attgcgaaaa gaagatacgc ccatcagcaa tagtattgct gaccatatcg
     9361 aaagctttgt cgattatgat tttgcccatc tgttattcag tgatggcagt gtcacccaat
     9421 ccattagctt ggaaaaacaa ctcaatatta ttcaagtggc agatttggtc ttacccgatt
     9481 ccaatagctc ttttgaagaa tacactacca tggaattatt aagtgtggca atgctgattg
     9541 tcattagtac ctttgcttta gactttatcc actctgaccg ttccattttt aaaattgttg
     9601 accttgatga ggcttggagt tttttacaag tggcacaagg aaaagctcta tctatgcgat
     9661 tggttcgagc aggacgtgcc atgaatgcag gggtctattt cgttactcaa aacgcggacg
     9721 atttactaga tgaaaagatg aagaataata ttggcttgaa atttgccttt cgttccactg
     9781 acctagtgga aatcaaaaag accttagaat tttttggtgt ggacaaggaa gatgaaaaca
     9841 atcagaaacg cttacgtgaa ttagaaaatg gacaatgttt agtttcagac atctatggtc
     9901 gtgtaggggt tatgcagttt cacccgattt ttgaagagtt gcttcatgcc tttgatacca
     9961 gaccgcctgt gcgaaatgag gtggaagaat gacacaaaaa caaaagaaat ttcttcacta
    10021 tagttggata acgctccttt gtgtgggcgt tattttactt ttattaggca cgctgggcaa
    10081 tgcagtacaa gcgacaggct tagttgatga aaccattgat acaagcaatg aatactccaa
    10141 gtatggtttg aatcaatatc agcttgatta ttatgtggac aatagctggg gctggcttcc
    10201 atggaattgg agtgatggca taggacaatc ggtgatgtat ggattgtatg ccattactaa
    10261 ttttatttgg acaatttctc tttatctatc gaacgccaca ggttatttgg tgaaagaagc
    10321 ctatgcctta gatttcatta gtcaaacatc ccaagcaatt ggcgaaaaca tgcagacgat
    10381 tgcaggcatt acaccgaatg gcttttccag ttcagggttt tatgtcggtt tcctccttct
    10441 ttttattctc attgttggaa tctatgttgc ctatacagga ttggttaagc gagaaacgac
    10501 gaaagtgata cgagctgttt taaactttgt cgtggtgttt attctatcgg cttcctttat
    10561 tgcgtatgca cccgattata ttgcgaaaat caatgatttt tccaaagatg tcagtaccgc
    10621 cagtctgaat attgggacaa aaattgttct tcctaacacg gatagtcaag ggaaagacag
    10681 tgtggacatg atacgtgaca gcttattctc tgttcaagta aagcaaccat ggctattgct
    10741 tcaatatggc tccactgatg tagaagcgtt aggggaagaa cgtgtggaaa gtttattgtc
    10801 taccagtcca gatacaaaca atggtgaaga tagagaaaat gtagtgattg aagaaattga
    10861 agatcatgac aatacctatt taaccctacc gaagacgatt tccagattag gaacagtctt
    10921 ctttttgttt gtattcaata ttggtatctc gatttttgtt ttccttctaa caggaatcat
    10981 gattttttcg caagtgctat ttattatttt cgctatgttt ttgccaatca gtttcctatt
    11041 aagtatgttg ccgagttttg atggcatggg caggcatgcc attaccaagt tatttaatgt
    11101 gattatgaca agggctggta ttaccttgat tattaccgtt gcctttagta tttccactat
    11161 gctttattcg ctgtcagcaa gttctccctt tttcatgatt atgtttttac aaattgtgac
    11221 ctttgcaggg atttatttta aactgggcga tttaatgagc ctattcactt tacaaagcaa
    11281 cgactctcaa aacatgggca gacaagtgtt tcgcagacct cgtcaaatga tgaatcgaca
    11341 aaccagaaga tttcaacgca gtatgagtaa gatttttcgt ggaaaagcag ggtcaacgaa
    11401 gcaaaagaca atgacaacaa agcagaatca aaccaataaa gctaaccata ctcgtcaaaa
    11461 tgaacggact acaccaaaga aagaaacagc actttccaat gtgaaaaagc aacaaggcgg
    11521 tatcgggcaa cgattaggtg aaaagacagc ccatgtattg gacacgaaaa atcgtctagt
    11581 caataaaaga aagcaggcaa aacaacagat acaggcgaca ccaacccatg tcaaatatgc
    11641 ccttcatcaa ggaaaagaga atgtcactca taatgtaaca gactttaaac aatctatggc
    11701 agataccaaa gctcaacgca aacaacagcg tatgaaccag caggagcaaa aacgaaagaa
    11761 cattgctcaa aagcgtttgg agctggataa agcaaaagaa aaaagacgaa aagaaaaccc
    11821 atcatctcct gttcagaata accagcagca tgttcgcaag cgtcagcaaa ccgtcacgcc
    11881 tgttttgtca caaaacacta aacgtgaaaa tcatcagcga ccattgaaaa aagagcaaaa
    11941 acaaaagaca ccaccgaagc aattcaatca gaggaagcta aataaaagaa cggataagcg
    12001 aggacaacaa acatgaagct aaaaataaca gtgatttgta gtgtggtctt ctctctcttc
    12061 ttttttctct tattattcct agtcattttc tttgcagatg atacggacag tggcgaaaac
    12121 aacaaggatt cttctattcc acaaggcggt gtgaccgttt caccagaagt gttggcacac
    12181 cgtccgttga ttgaaaaata tggtaaggaa tatgggattg aagactatgt ttcttatatc
    12241 ttagccatta tgcaagtgga atcgggcggt acagcagaag atgttatgca aagttccgaa
    12301 tctttgggtt taccgcccaa cagtttgagt accgaagagt ccatcaaaca aggggttaaa
    12361 tatttcagtg aattattaac cagtgcagaa caacaaggag tggatataga ttctgttatc
    12421 caatcttata attatggcgg tggcttttta aattatgtga ggagtcatgg gaaaaaatat
    12481 acctatgaat tagccgaaca gttttctaaa gagaagtcgg gtggtcaaaa agcagactat
    12541 cccaatccta tagccatacc tgtgaatggt ggttggcgat ataactatgg aaatcagttt
    12601 tatgttcagt tggtttcaca atacctaacc gacacttccc caacagaatt tgatgatgaa
    12661 accgtgcaag tcattatgga cgaagccttg aaatacgaag ggtttcccta tgtctttgga
    12721 ggggcgtcgc ctaccacctc ttttgattgt agtggcttaa tacaatgggt gtatgacaaa
    12781 gctggcattt ctctccctcg tgtcgcccaa gatcagtatg acgctacgca agaaatctct
    12841 atggaagaag cccaagcagg cgaccttatc ttctttcatt caacctataa tgcaggcaca
    12901 tatgtgactc atgtggcgat atatttagag ggaaatcgct tttatcatgc aggcgaccca
    12961 atcggttatg gcgacttaag cagtcgttat tggcaagacc atctgattgg ggctagacgt
    13021 gttattcata actaagaaag gaatgaatca aatgattata aaaatcgagc gaaaagaaaa
    13081 gaaggagaaa ccaaagaaat ctaaaaaacc gagcaagcaa agaaaagttc cgatgattaa
    13141 agttggcacg cataagaaat taacctttgt tttatgggtt ttattgattg gtagtgtcgg
    13201 atttggaatc tataaaaatt ttactgccat tgacactcat actgtccgtg aaacagaaat
    13261 cattaaacag cagattgtcg ataccaacca agtagaaagt tttgtgaaat cctttgcgaa
    13321 agactacttt tcgtggcaac aatcccaaga agccattgat aaacgaaatg aaaaattgac
    13381 ccattatttg acggaagaat tgcaggtatt aaatgaagaa atgattcgta aggatattcc
    13441 tactagttct agtgtgaacg atatacaggt gtggcaggtt tctcaagtga atgagaacac
    13501 ctttgaggta ctgttttctg ttgaacaagt tattaccgaa gacaaagaca aggaaactat
    13561 atcgtctagc tttcatgtgg ttgtccatat agatgaatca gataatatgg ttattatcaa
    13621 aaatccgaca atgagtaaga aacctcaaaa atcggactac caacccaaac agctagaaag
    13681 tgaccataca gtagatacag aaacgatgga cgaaatcact agctttttag aaacgttctt
    13741 ccagctctat cctaccgcca cagaaaaaga attgacttac tatgtaagta atcatgtgtt
    13801 acctatgatt aacaaggaat atgttttcga ggaattggtg aatcctatct ttaccagaaa
    13861 agataaccaa gtaatcgtga atgtcgctgt gaaatattta gatcaagaaa caaaggcaac
    13921 ccaaatctcg caatttgagt tgatattaga aaaacaagat aattggaaga ttgtaaaata
    13981 acaaatattg gtacatgatt acagatactt tgtaatcatg tactcttttt gataaaaaat
    14041 tggagattcc tttacaaata tgctcttacg tgctattatt taagtgacta tttaaaagga
    14101 gttaataaat atgcggcaag gtattcttaa ataaactgtc aatttgatag cgggaacaaa
    14161 taattagatg tcctttttta ggagggctta gttttttgta cccagtttaa gaataccttt
    14221 atcatgtgat tctaaagtat ccagagaata tctgtatgct ttgtatacct atggttatgc
    14281 ataaaaatcc cagtgataaa agtatttatc actgggattt ttatgccctt ttgggttttt
    14341 gaatggagga aaatcacatg aaaattatta atattggagt tttagctcat gttgatgcag
    14401 gaaaaactac cttaacagaa agcttattat ataacagtgg agcgattaca gaattaggaa
    14461 gcgtggacaa aggtacaacg aggacggata atacgctttt agaacgtcag agaggaatta
    14521 caattcagac aggaataacc tcttttcagt gggaaaatac gaaggtgaac atcatagaca
    14581 cgccaggaca tatggatttc ttagcagaag tatatcgttc attatcagtt ttagatgggg
    14641 caattctact gatttctgca aaagatggcg tacaagcaca aactcgtata ttatttcatg
    14701 cacttaggaa aatggggatt cccacaatct tttttatcaa taagattgac caaaatggaa
    14761 ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa attgtaatca
    14821 aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat
    14881 gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc ggtaaatcat
    14941 tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt tctctgttcc
    15001 ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata gaagttatta
    15061 ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga aatgttttca
    15121 aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag ttttggagta
    15181 caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt
    15241 ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta
    15301 tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt
    15361 tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata
    15421 agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat
    15481 acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt
    15541 tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg
    15601 aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag
    15661 ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag
    15721 atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa
    15781 tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt
    15841 actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa
    15901 aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta
    15961 tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt
    16021 tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag
    16081 atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat
    16141 tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc
    16201 aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt
    16261 atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt
    16321 cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg
    16381 agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa
    16441 caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg
    16501 tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa
    16561 cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct
    16621 atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc
    16681 atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac
    16741 catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta
    16801 ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact
    16861 attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact
    16921 tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac
    16981 atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg
    17041 gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc
    17101 tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag
    17161 aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc
    17221 cattagccac taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc
    17281 tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct
    17341 ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg
    17401 gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta
    17461 caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt
    17521 tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt
    17581 atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt
    17641 tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat
    17701 agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt
    17761 cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga
    17821 tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa
    17881 aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac
    17941 aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg
    18001 ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat
    18061 ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca
    18121 ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa
    18181 ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt
    18241 tagttgaata agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat
    18301 tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat
    18361 gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa
    18421 aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat ttacgagatt
    18481 cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact tcaataaatg
    18541 gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg caaaatgagt
    18601 ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga aaaaagattg
    18661 aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa cagagagaaa
    18721 tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga tattacgtgg
    18781 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
    18841 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
    18901 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
    18961 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
    19021 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
    19081 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
    19141 gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt cggatgctta
    19201 ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta gagccatatc
    19261 ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac gatgctccca
    19321 aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt attattggag
    19381 aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt acaaatgggc
    19441 ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa cctgtttgcc
    19501 agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat aaaataactt
    19561 agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga aatattcttt
    19621 aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta caagtggata
    19681 ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac tatacttaaa
    19741 aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt tcaaaaaatg
    19801 aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt tgagaaatga
    19861 aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc cttcaatatt
    19921 tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt tgtctgtaaa
    19981 gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat tatctgtagg
    20041 taagaaaaat tcatcaatag atacatggag taatgataca agatcataca gcacttgtgt
    20101 acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa tttcaatcat
    20161 tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa tcgctaaacc
    20221 aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc tctatacatt
    20281 ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag tttatgagtt
    20341 cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata gaaaggtcgc
    20401 tgattttatt tacctataca actttataaa atttagtcat actcctagaa agtagataaa
    20461 attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca tctagttctc
    20521 ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat gaccatcaat
    20581 gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga aaacaatctg
    20641 tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc atttatgcaa
    20701 caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca catgccactc
    20761 cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa gaaaccatag
    20821 aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa gattacttca
    20881 aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat aactaccttg
    20941 tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt aaattatttg
    21001 gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat ctttcagaga
    21061 aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg gagattgcgg
    21121 agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga ctagccatga
    21181 ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg ttcccttccc
    21241 cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg ttaaacatta
    21301 tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg gaaattctca
    21361 tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct ctaagatttt
    21421 atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg tatccacgct
    21481 tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt aggttttgat
    21541 aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata cgtttcggta
    21601 agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg attcatgtta
    21661 gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg tgataatgat
    21721 actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt acggctacat
    21781 gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga ttgctaagat
    21841 attcttctct tgtcagtgta ttattataaa actatttgat ataattatag gtgaatattg
    21901 gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac aaaaaaggat
    21961 ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa agcaaaagaa
    22021 ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag agtccctcgt
    22081 gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc tgagtagttt
    22141 ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca aagaatggaa
    22201 cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt caaaaatatc
    22261 gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg ctattgatga
    22321 aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat atctttgaaa
    22381 ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat cgcttaccaa
    22441 ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt gagcctattg
    22501 atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat gtaagagctg
    22561 ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata accaccacaa
    22621 atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa ttttggacaa
    22681 aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat caacactttt
    22741 tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa gctactgcca
    22801 ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa acactctatt
    22861 acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc gttcgtcata
    22921 ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta caacaaaatc
    22981 taattcagac agactttgta atgagctata acggtattcc tacccaaaaa catacactat
    23041 catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga atccatgggt
    23101 tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt atcattaaag
    23161 atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat ctttatccta
    23221 acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa gaagcaaaac
    23281 aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga aacaaagaaa
    23341 tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac ccttggtata
    23401 agaggatttc tagctctcct acaactattc ccactccact gttgctggtg gtttagaagt
    23461 gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta cagagatttt
    23521 ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga ttgaagttac
    23581 agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac cgacagagcg
    23641 gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag cagctgcgat
    23701 ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt cagtgatttc
    23761 gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa cgattgaatc
    23821 aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg tgtttaatgg
    23881 ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt gagatttgat
    23941 cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc cttgtgctag
    24001 gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa
    24061 ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca acttgtctaa
    24121 gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc ctagactttc
    24181 cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa
    24241 ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa cgccacctga
    24301 aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta cttgcatatc
    24361 gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg caaagtggcg
    24421 cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga taccataaaa
    24481 attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc cgactgtttc
    24541 aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg tttgttgttt
    24601 tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg ctttcccata
    24661 ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca attgcatacc
    24721 atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga tcccaaatgc
    24781 attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg gattcatcgc
    24841 ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc caagatcacg
    24901 aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga taatcttttc
    24961 aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt taaattttat
    25021 ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc gaatcgatga
    25081 tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc aagatatatt
    25141 cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta aaggcttctt
    25201 cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa gccgtatcca
    25261 gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg gcatcgacat
    25321 agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt aattgaagcg
    25381 tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac ttgtctggaa
    25441 tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga ccagatttta
    25501 cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat cctttacggt
    25561 ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca gtcgtgatca
    25621 gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg
    25681 tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt acttctacgt
    25741 atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc atataaagat
    25801 aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa atgcggtcat
    25861 tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca ttacgataaa
    25921 attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc atattagcct
    25981 cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac cccaccactt
    26041 aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg agtcttattc
    26101 ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa tgagaaggat
    26161 ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg attttgaaca
    26221 atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg gtgtattttc
    26281 acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt gggaagagtt
    26341 acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg ggttagcttt
    26401 ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct
    26461 agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc aatcgaatat
    26521 ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc cgatccgtgc
    26581 tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga agaagggcgg
    26641 tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga
    26701 agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata tcatcaaaag
    26761 tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca
    26821 tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa agaactattt
    26881 tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa ataaataaga
    26941 tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac
    27001 atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat agggaattta
    27061 gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa atggcaggcg
    27121 tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt agtgataaga
    27181 caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat tatttcgctt
    27241 caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat gtcactgatt
    27301 tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg ggctatgtga
    27361 tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta caagagcttt
    27421 cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct gagaaatatg
    27481 cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc ggattgaatc
    27541 gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc
    27601 tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact ggatactatc
    27661 aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc ctgcctttta
    27721 atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact gcatcaaaaa
    27781 aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc atggcgatcg
    27841 gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc tcagtcatag
    27901 gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca gtgcagcagc
    27961 caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc gaatttcctc
    28021 aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag
    28081 tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca aaggctagtt
    28141 agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt cctaaaaata
    28201 atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg atctgattga
    28261 aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg tcacgaacta
    28321 tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa ttttttatca
    28381 agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt ctggtgatgt
    28441 catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag gtggcgttgg
    28501 tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga cagtggctaa
    28561 aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag aagcaattcc
    28621 cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg aaagtaaagt
    28681 agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt atgccttgcg
    28741 tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta tgagtaaaaa
    28801 aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg gtgctttcat
    28861 gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg ggaaactggc
    28921 taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg aagctatcgg
    28981 aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc cagaagatct
    29041 agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca aagctaagac
    29101 aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa
    29161 attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac atccagaacg
    29221 aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag ttgtgtcaaa
    29281 aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa
    29341 agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg gtgacactgt
    29401 gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca cttctgtcat
    29461 tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa cgttgattca
    29521 tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa
    29581 agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg catctaaaga
    29641 agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg tctgcatcaa
    29701 tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag ccgtatgtac
    29761 agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg aaacaacaga
    29821 tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg ctttgaaatc
    29881 aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag caaaagatcg
    29941 tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa tcatccgagc
    30001 ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg gtttctcaac
    30061 aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac cagaaatggg
    30121 tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga tcgaagctgg
    30181 tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg
    30241 ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg gatcaacaca
    30301 gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt aggcgcttgt
    30361 ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc atcatttgga
    30421 ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg agaaaaaaca
    30481 tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg tggtgcatgt
    30541 cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt aggagacaat
    30601 ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat
    30661 ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt attttaggag
    30721 gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca ctagcagcag
    30781 ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt ggcaaaagct
    30841 catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac gaccgttcat
    30901 tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga ctttcacgag
    30961 gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc ccaaatattg
    31021 accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa ctaaaaccag
    31081 cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc gatgatgtca
    31141 tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct tcttatcttg
    31201 caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc ggcggtgtaa
    31261 aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat gcaggtgcga
    31321 aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc tctgctccag
    31381 ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc atcttccatg
    31441 cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg
    31501 acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc gaatacacat
    31561 taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt ggaacagtag
    31621 tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa cacactgttt
    31681 atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac gaagcgaaaa
    31741 aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc
    31801 ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac aaggctatgg
    31861 tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt tggataaggg
    31921 aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc gtgaatctta
    31981 gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat
    32041 attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc aaatgacaac
    32101 atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa cggtgctggc
    32161 aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg gaaaattttg
    32221 atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt agggattggg
    32281 atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa catcgtgttg
    32341 ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa agagatccaa
    32401 aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg tgatatttca
    32461 gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc agatgtactg
    32521 atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc
    32581 atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa gttagatgaa
    32641 atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc
    32701 aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag aacggtttca
    32761 ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat tgaaaatcta
    32821 gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga ggttcgtgct
    32881 ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt gatccaagct
    32941 ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga agacatcact
    33001 aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga agaccgtcat
    33061 aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca aacctatcat
    33121 caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga acatgccaga
    33181 gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta
    33241 tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga tcctgatctg
    33301 ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt tattcataaa
    33361 cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt cgaattagaa
    33421 gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat tgtcggtatc
    33481 gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg ctatacctta
    33541 gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa
    33601 attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct taggcgcgat
    33661 attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc tagatgcagc
    33721 ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt tgatttttac
    33781 agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc tttctggaca
    33841 agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag atctgccaaa
    33901 agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga tggcagcggc
    33961 tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa caatcatgat
    34021 gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc ctgaaagcta
    34081 tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc ggttaccatg
    34141 gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag caatcatcgc
    34201 gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa tccgttctgt
    34261 tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt
    34321 gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag gattaggaac
    34381 gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg gaatggctgt
    34441 ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct tgttcagtat
    34501 tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg agatcgtcaa
    34561 tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc gtttcgtcat
    34621 ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc
    34681 aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt aattgccttg
    34741 atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact aggcggaact
    34801 ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt catgggtgct
    34861 tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat gacaccatgg
    34921 atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca cgcagtagca
    34981 acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct gatggcacct
    35041 gctttaggtg ttttcttgat caaagcatgg tatggtaaag gtcaaactga taacatcacg
    35101 cagaatctgg ggtatttttc tttcccagga ttagcagata ttccggtcat tggaccaatt
    35161 ttctttaaaa atacattctt acctgcttat tgtgctattt tgattgctgt cctagcttgg
    35221 gtgatcatct tcaagacgaa attcggcttg cgcctccgtt cagttggtga aaatcctcaa
    35281 gctgccgaca cattaggtat caatgtatat gctatgcgtt attcaggcgt actgatttct
    35341 ggcttgttag gcggtatggg cggtgctgtt tttgctcagt cgatttccgg taacttttca
    35401 gctggtacga tcgttggaca agggttcatc tccatggctg ctatgatctt tggtaagtgg
    35461 aatcctttag gggcaatggg tgcctcattg ttctttggtt ttgctcaaag cttgagtatc
    35521 attggcgcac aattaccagt tatttcttcg attccgccag tattgctgca aattgctcca
    35581 tatttgctga caatcattgt gctggttgtt ttcttaggga aagcttctgg tccaaaagca
    35641 aacgggaaga actatatcaa atcaaaataa tcttttagcg aataaaaagg gtgggttacg
    35701 ggaagtgttt ttcgtctcgc cctttttgtt ttaaaggcaa taaaaataaa aagaaagaag
    35761 gaataatcat gtttaaacgc gtacatctga tcgtcatgga ttcagtagga attggagaag
    35821 caccagatgc cgataagttc ggtgatgttg gttctgatac attaggacac attgcgaaag
    35881 aagcaggatt gaacgttcca catttagagc agcttggttt aggaacgatc cgtcctcttg
    35941 aaggtgtgga atcagtgaaa gatcatcaag gctatgcgac gaaattagaa gaagtttctg
    36001 tagggaaaga tacgatgaca ggccactggg aaatcatggg attgaatatc aaaactccat
    36061 ttcgtgtgtt tcctaatgga ttccctgatg atctgatcaa acaaatcgaa gaattttctg
    36121 gccgtaaagt cgtatgtaac aaaccttata gcggaacagc tgttattgac gattacggtg
    36181 aacaccaaat gaaaacaggc gatttaattg tatatacttc tgccgatcct gtccttcaga
    36241 tcgcggcaca tgaagaaatc attcctttag aagaattata tcggatttgc caattcgttc
    36301 gtgatataac aaaagatgat ccgtatatga ttggccgaat cattgcacgt ccgtacgtgg
    36361 gtgaaccagg agacttcaca cgaacaagca atcgccatga ttatgcgttg aatccattcg
    36421 gacacacagt ccttgattct ttgaaagaag caggaaagga cgtgattgct gttggaaaga
    36481 tcaacgatat tttcaacgga caaggaatca ctgaatctgt ccgcacgaaa agcaatatgg
    36541 atggcgttga ccagctgctg aaagttatga aaaacgattt ttcaggaatc agctttacca
    36601 atttagtaga ttttgatgca ttatacggtc atcgtcggga tgtagtcggc tatgcgcatg
    36661 cgattgaaga atttgactta cgaattccag aattgcttga agcgatggaa gaagacgact
    36721 tgctaatgat tacagccgat cacggaaacg atccgacgtt tactggtacc gatcatacgc
    36781 gagaatatgt accgttattg gtttacagta aaaaaatgaa agaacaaggg gcattgcctc
    36841 aaggatatta ttcagatatt tcagcaacga ttgctgagaa ctttagtgtt tcttcgactg
    36901 aaaatggcga aagcttcttg aaattattaa aataatcttt ttaaatagga gaaagcaaat
    36961 gaccaagtta agcgagatgt tacaagaaac gacccagttt atccttgata aaggagtaaa
    37021 agaaattgat tttggtttga ttctaggttc aggattaggt gaattagcag atgaaatcga
    37081 agatgctgta atgatttctt atgatgagat cccgttcttt cctgtttcga cagtagttgg
    37141 gcatgcagga aagctagttt atgggtctct ggcaggcaag aaagtgttag ccatgcaagg
    37201 gagattccac tactatgaag gtcattccat gcaaacagtg acctatccag tacgtgtgat
    37261 ggccggacta ggggcccatt cagtcattgt gactaatgca tgtggtggtg tcaacgaatc
    37321 atttgtacca ggagatttga tgctgattac cgatcatatc aattttactg gtgaaaatcc
    37381 tttgatcggt ccgaatgaag atgcaatggg ccctagattt ccagatatga gtcaagctta
    37441 tacgcctgac tatcaagcaa cagcaaaagc agcagcagaa aaactaggtt tggatcttaa
    37501 agaaggcgta tatatgggct attctggacc aacttatgaa acgcctgcgg agatccgaat
    37561 gtctcggaca ataggagccg atgcagtagg gatgtcgact gttccagaag tcatcgtagc
    37621 agctcacagc ggattaaaag tgttaggaat ctcttgtatt accaacttag cagcaggtat
    37681 gcaagcgaat ctgagccatg aagaagttgt agaaacaact cagagagtca aacaatcttt
    37741 taaagcattg gttaaagaaa cattgacatt attgtaatag gaggaacaat aaaatgagtg
    37801 tgcatattga agcgaaagaa ggcgagatcg ccgataagat tttacttccg ggagatcctt
    37861 tgcgagctaa atatattgcg gagacttttt tggaagatcc agtttgttac aatcaggtac
    37921 gcggtatgtt aggctttacc ggcaaatata aaggagaacg tatttctgtt caaggaacag
    37981 gaatgggaat gccatcagcc accatctatg cccatgaatt gatccagtcg tatggtgtaa
    38041 agaaattgat tcgagtaggt acatgcggtg cattgtcaaa agacgtccat gttcgtgatt
    38101 tagtactagc acaaggtgca gcgaccagtt cttcgatgat cgaaaaaaat ttccaagcct
    38161 ttcattttcc tccaatcagt gatttcaatc tattactgaa agcatatgag atagctaaag
    38221 aaaagggcta tacggttcat gtaggaaatg ttctttcgga agattctttc tacaaagatg
    38281 atttgacaga gacgttccaa ttagctgaac ttggcgtttt aggagtggaa atggaagcag
    38341 cggctcttta ttacttaggc gcgaaatacc atgtgcagac attaagtttg atgaccgtta
    38401 gcgaccattt gatcaccggt gaagaaacaa cagctgcaga acgtcaatct acgttcaatg
    38461 aaatgatcga agtaggattg gaaacagcga tagcagaata agaaggaaat gaaaaagctg
    38521 ccctgcatta agtaataaga acatccaagc aaaaatagct cctcatgttt ttgacttggc
    38581 tggacttatt attgcaaatg taagcttttg aacaccgttt accaagaggt tgtgaatctg
    38641 ccgtttaact ctgagtcaca aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat
    38701 attgtgactt gtgaccgaga agttggcagg agaaaaccgt ttatacgaaa aaagggactg
    38761 tgacaaatcg tgtcacagtc ccttttttat agtgtttttt ataggtagta ttttttagca
    38821 actgttaagt catcatttaa ttcataaaca agtggttgac ctgttggaat ttcaagatcc
    38881 atgatgtctt cgtcagagat accttcgata tgtttagcta gggcgcgtaa tgagttacca
    38941 tgtgcagcta ctaaaacagt tttgttgtct agtaatgctg gagcaatttc atcttgccag
    39001 aatggcaaag cgcgttctaa tgtcactttc aagttttctc caccaggaat atcgcgtttg
    39061 tctaacattg cgtaacgacg gtcatttgct gcagaacctt cgtcagtttc ttccattaat
    39121 ggaggaagag tatcgtaaga acgacgccag atgtgcactt gttcgtcgcc gtatttttca
    39181 gctgtttctt ttttgttcaa accttgtaat ttaccatagt gacgttcgtt taagcgccat
    39241 gatttgattt gtggtaccca aagttgatct gaatattcta agatcaagtt acatgttttg
    39301 attgcacgag ttaaaacaga agtgtaagca acatcaaatt cgattccagc ttctttgatt
    39361 ttgcgtccgc cttcttttgc ttcttcgatt ccttctggtg ctagatttac atcagcccaa
    39421 ccagtaaatt ggttcaatgc attccattca ctaagaccgt gacgagaaaa aactaatttt
    39481 ggcatgttta gctctccttt taagataggt ttatatttct gaaattttca gttctgtttt
    39541 attgtacaat tttttggaag agatttccat cgaaaaatta gataagattc tatgagaaaa
    39601 caagtagaga atataaaaaa acgaacagtg cccacgcaac tgttcgtttt taggtattag
    39661 atgcataatt gctcgatttc tttttcaggt aaattgatat atttataaca tgttccaact
    39721 gaaacaccgc attttgaaga aataaattgg atcgtttctt ttttttcata atagagacgt
    39781 cggatttttt tgatcgtttt tgcatcgatt tttggtcttc cgccgatttt tcctttttta
    39841 cgagcttcat ctaatccaac taatgtacgt tctttgatca atgcgcactc catgtttgct
    39901 aggttttcca ttaattggaa atagattttt cccatttcgc gcgtatcgat tccttcatca
    39961 agactaatca agtgcagccc gcgatctttg aataattgcg tgagttctgt taattgccgc
    40021 gtagatttgc ctaatcgatg aagttggcaa atgactagtg catcgccttg ttgcatgtca
    40081 gcgatcactg actccaaatg aacagcagta gaatcatttt gttccacttc atatgtctca
    40141 tggaaaattt cttgacaacc aaagtcagat aattgatcaa cctgagttgt taaatcaacg
    40201 tcatttatgg ttgtgcgtgc ataaccaata attttcatgg tgattccttt ctagcagtga
    40261 ttttctaact ggatataaca gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt
    40321 tcgcaaattt tccgctgtct ttttttattg gatgatcaag cagtttttct ttatttacga
    40381 cggattgcgt caagaatctc aacgcctttt tctgagccga caactacttt agaaaccatg
    40441 tttaggaaaa gcccatgttc tactacccca acaagttcat ctaaataggt agctaagctt
    40501 tctggctgtt cgatgacttc tagatgcaga tcaatgatgt aatgaccacc atctgtggtt
    40561 aagacttctc cgttttcgtc tgtacgcaag acgggttgga atcttttttc ttcaaaaata
    40621 tgaagtagct gttgggagcc ataagggatc acttcaactg ggagtgggaa cttcccaagt
    40681 ttatcgacta atttagaaga atcaacgatc caaatcgttt cttttgagta cgtagcgact
    40741 attttctcga aaagcaaagc agcgccgccg cctttgatcc cttgaaaatc agcactgatt
    40801 tcatcagcac cgtctattgt caaatcgact acggggactt catcaatact ctttaacggt
    40861 attcctaatt tttccgcttg ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt
    40921 aaaccttctt cacgcatacg gcgaccaatc tcttctacca tataataggc ggttgaaccg
    40981 gtcccgagac ctacgatcat cccgtccttt acgaatttcg cggcttcaat gccagctaat
    41041 tgttttaaat tcatctttgc gtccaccttt ttttagtttg agcatcgtgc tttcctcact
    41101 attttagcgt gatttacggt aaaaagatac cattcttttt tattccaata aagctttttc
    41161 aaaaaaaatt gcatctcttg ttgacatgtt ttttcattcg tgatattcta acaaaggtgc
    41221 tttatctaca ggtttctgtg aagaaacgtg ctgactgttt atcaaagcat atttgataag
    41281 gatgcagaca ttgcatttat tttttatcac aaaaaaagaa ccacctggat gtgtggaact
    41341 agacgcaaat cgtgggaagg aggaaacaag gaatgcctac aattaaccaa ttagtacgta
    41401 aacctcgtaa atcaaaggtg gaaaaatcta attcacctgc tttgaacaaa ggatataaca
    41461 gctttaagaa aactcaaact aacgtaaact cacctcaaaa acgtggggta gcaactcgtg
    41521 tgggtacaat gacacctaaa aaaccgaact cagctttacg taaatatgca cgtgttcgtt
    41581 tgtcaaactt aattgaagtt actgcttata tcccagggat tggtcataac ttacaagaac
    41641 acagtgttgt attattacgc ggtggtcgtg taaaagactt accaggggta cgttaccata
    41701 tcgttcgcgg tgctttagat actgctggtg ttaacgaccg taaacaaagc cgttctaaat
    41761 acggaactaa aaaacctaaa gcttaatttt aattattaaa caaatgaaac ttgcttctac
    41821 tatatagaag taattactat gaacaatatt tcggaaggag gagttacgga tgccacgtaa
    41881 aggtcctgtt gcaaaacgtg atgttttacc agatcctatt tataactcaa aattagtaac
    41941 tcgcttgatc aaccgtgtaa tggttgacgg aaaacgcggg attgcagcta acattatcta
    42001 taatgccttt gacttgatca aagaatctac aggtaacgat ccattagaag tgtttgaaca
    42061 agcaatgaaa aacgttcaac ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa
    42121 ctaccaagta ccagttgaag ttcgccctga acgccgcgtt actctaggtc ttcgttgggt
    42181 agtaaactac gctcgcttgc gcggtgaaca cacaatggaa gaacgcttag caaaagaaat
    42241 catggatgct gcaaacaaca caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat
    42301 ggctgatgcc aaccgtgcgt ttgctcacta tcgctggtaa gattctctct gtttggtcgg
    42361 cttttataag tcgacctcag atagtgtcct tatttttagc aactagatag taaagagagg
    42421 agaaacaagg agaatgacca gagaattttc tttacaaaac actcgtaata tcggtatcat
    42481 ggcccacatc gatgctggta aaacgactgc tactgaacgt atcttgtact atactggtaa
    42541 aatccataaa attggtgaaa ctcatgaagg agcttcacaa atggactgga tggaacaaga
    42601 acaagaacgt ggtattacga tcacttctgc tgctactaca gcacaatgga agggtcaccg
    42661 aatcaacatc atcgacacac caggacacgt agacttcaca gttgaagttg aacgttcatt
    42721 gcgtgtactt gatggtgctg taactgttct tgatgcacaa tcaggtgttg aacctcaaac
    42781 tgaaacagtt tggcgtcaag caacaactta tggtgtacct cgtatcgtat ttgcaaacaa
    42841 aatggacaag atcggtgctg acttcttata ttcagtatca actttacatg accgtttgca
    42901 agcaaacgct cacccaatcc aattaccaat tggtgcggaa gacgacttca caggaattat
    42961 cgaccttgta acaatgaagg ctgaaatgta tacaaacgat cttggaacag agatcgaaga
    43021 aacagaaatt cctgaagaat accgcgaatt agctgaagaa tggcgcgaaa aattggttga
    43081 agctgtagct gaaactgatg aagaacttac tttgaaatac ttagaaggtg aagaaatcac
    43141 tgaagctgag ttaaaagaag gtatccgccg cgcaacagtt aacgtagaat tctacccagt
    43201 actatgtggt tcagcattta agaacaaagg ggttcaatta ctattggacg ctgttcttga
    43261 ttacttgcca tcaccgttag atatcccagc tatcaaaggg attgacccta aaactgatga
    43321 agaagtagaa cgtcctgcag acgacagcgc tcctttctca gctttagcat ttaaagttat
    43381 gactgaccca ttcgtaggtc gtttaacttt cttccgtgtg tactctggtg ttttacaatc
    43441 aggttcatac gtacaaaacg caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca
    43501 gatgcatgcc aactctcgtt cagaaatctc tgaagtttac gctggagata tcgctgctgc
    43561 tgttggtttg aaagacacaa ctactgggga cacactatgt gatgaaaaga gtctagttat
    43621 tcttgaatcc atggaattcc cagaaccagt tatccaagtc gctattgaac ctaaatcaaa
    43681 agctgaccaa gataaaatgg gtgtggcttt acaaaaacta tctgaagaag atcctacttt
    43741 ccgtgctgaa acaaacgtgg aaacaggcga aacaatcatc gctggtatgg gtgaacttca
    43801 cttagatatc atcgttgacc gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc
    43861 gcctcaagta tcttaccgtg aaactttccg tgctggcact caagccgaag gtaagtttgt
    43921 acgtcagtct ggtggtaaag gtcaatacgg tcacgtatgg attgaattta caccaaatga
    43981 agaaggtgct ggtttcgaat ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata
    44041 cattccagct gttgaaactg gtttgaaaga tgctatggaa aatggtgtct tagctggcta
    44101 tccattagta gatatcaaag ctaaacttta cgatggttca taccatgatg tcgattcaaa
    44161 tgaaactgcc ttccgtgttg ctgcttctat ggcattacgt gcagcagcta agaaagctaa
    44221 tccagtaatc cttgaaccaa tcatggctgt agaagttgtt atccctgaag attatttagg
    44281 tgatgttatg ggtcacgtaa ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc
    44341 tggcggccaa caagttgttc gtgcgatggt tccacttgct gaaatgtttg gttatgctac
    44401 aactcttcgt tctgctactc aaggtcgtgg tacatttaca atgacatttg accactacga
    44461 agatgtacca aaatcagtac aagaagaaat cattaagaaa aacggcggaa aagctgaata
    44521 atttcttaaa accggatagt cctgcaaaga gattatccaa agagactata tttttaatat
    44581 agtttcgtgt aaaataatta tcgatgatat gggcatgtgg tgtaccttgt gtacatcata
    44641 tacctatcag acaaataaaa aatatattgt atttttagga ggacatttta aaatggcaaa
    44701 agaaaaattt gaccgttcta aaccacacgt taacattggt actatcggac acgttgacca
    44761 tggtaaaact acattaacag ctgcaatcac aactgtacta tctaagaaaa acggcggcca
    44821 agctatggct tacgatcaaa tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat
    44881 ctcaacagct cacgttgagt atgaaacaga tacacgccac tacgctcacg ttgactgccc
    44941 aggacacgcg gattacgtta aaaacatgat cactggtgct gctcaaatgg acggagctat
    45001 cttggtagtt tctgctgctg acggcccaat gcctcaaact cgtgaacaca tcctattgtc
    45061 tcgtcaagtt ggtgttcctt acatcgttgt attcttgaac aaagtagaca tggttgatga
    45121 cgaagaatta ctagaattag ttgaaatgga agttcgtgac ctattaacag aatacgaatt
    45181 ccctggtgac gatgttcctg tagttgctgg atcagctttg aaagctctag aaggcgacgc
    45241 ttcatacgaa gaaaaaattc ttgaattaat ggctgcagtt gacgaataca tcccaactcc
    45301 agaacgtgac aacgacaaac cattcatgat gccagttgaa gacgtgttct caattactgg
    45361 acgtggtact gttgctacag gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt
    45421 tgaagttgtt ggtattgctg aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt
    45481 ccgtaaattg ttagactacg ctgaagctgg agacaacatt ggtgctttac tacgtggtgt
    45541 tgcacgtgaa gacatccaac gtggacaagt tttagctaaa ccaggtacaa tcacacctca
    45601 tacaaaattc tctgcagaag tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc
    45661 attcttcact aactaccgtc cacaattcta cttccgtaca actgacgtaa caggtgttgt
    45721 tgaattacca gaaggaactg aaatggttat gcctggcgac aacgtaacaa tggaagttga
    45781 attaatccac ccaatcgcta tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg
    45841 tacagttggt gccggtgtcg ttacagaaat caaagcttaa ttcacttaag ttttaaagta
    45901 aagcaacatt tattcggacg taatcgctcg gaacgaaggt cttaggatct ttgttccgag
    45961 cttttttatt tattgcagga tcaggaaaaa agacaggagt attataaata gaagaaacac
    46021 gaaataaaag agtcagtatt ttctaaatga aactttatga aaacaaaatg ttttttacaa
    46081 gaaaaagcct tgctttgtga gtttatatat attataatga caaaggtgcg tttactttag
    46141 tgaattgcac agacgtaatc agaatgattt cgtcggctaa gaaacgagag gttgcgacac
    46201 gcccggacgc tttgccacga acgagcgtga cggaaatttt cgtggagcta tgtctacttt
    46261 tcaaaatagg cgaaggaggg aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag
    46321 cgtatgaaca ccgtatttta gatcaatcag cggataaaat cgtggaaact gcaaaaagaa
    46381 ctggagctag cgtgtctggt ccaattccat taccaactga acgcagtctt tacacaatca
    46441 tccgtgcgac tcataaatac aaagattcac gcgaacaatt cgaaatgcgt acacacaaac
    46501 gtcttatcga cattgtgaac ccaacaccaa aaactgttga tgctttgatg aagctagact
    46561 taccatctgg tgtaaacatc gaaatcaaat tataaaacta aaacaaaaat aatggaggtg
    46621 tactcatgac caaaggaatc ttagggaaaa aagtgggaat gacacaaatc ttcactgaat
    46681 ctggtgaatt aattccagta actgttgtag aagctactcc aaacgtagta ctacaagtga
    46741 aaacaatgga aacggatggc tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag
    46801 ttttatcaaa caaacctgcg aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct
    46861 tcattcgtga attcaaaaat gttgagctag gagaatatga agtaggaaaa gaaattacag
    46921 tagatgtttt ccaagcagga gacattattg atgtaacagg tactacgaaa ggtaaaggat
    46981 tccaaggggt tatcaaacgc cacggacaaa gccgcggacc aatggctcac ggttctcgtt
    47041 accatcgtcg tcctgggtca atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac
    47101 gtcttgctgg ccgtatgggt ggcaaccgcg taactatcca aaacttggaa gttgtacgtg
    47161 tcgatgcaga aagaaatgtt atcttgatca aaggtaatgt tcctggagct aaaaaatctt
    47221 taattactat caaatcagct gtgaaagcta aataattgta ggaagaaagg aggaactaag
    47281 gaatgccgaa tgtagcatta ttcaaacaag atggaagcca aaacggtgaa atcacactta
    47341 acgaagaaat cttcggaatt gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc
    47401 aacgtgcgtc attaagacaa ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg
    47461 gcggacgtaa accatggcgt caaaaaggaa caggtcgtgc acgtcaagga tctatccgct
    47521 caccacaatg gcgtggaggt ggcgtagtct ttggaccaac accacgttct tacagctaca
    47581 aacttcctaa aaaagttcgt cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg
    47641 aaaacaactt ggtagcaatc gaaggcttga acttcgacgc accaaaaaca aaagaattca
    47701 aacaagttct tgctaactta tctatcgact ctaaagtatt ggttgttcta gaaaaaggta
    47761 acgactttgc tgctttatca gcacgtaact taccaaacgt ttctgtagta acttctgata
    47821 atgtgagtgt gctagacgtt gtatcaaata caaaagtgtt agcaacacaa actgctctta
    47881 ctcaaattga ggaggtgctt gcataatgaa cttactagac gtaatcaaac gcccagtgat
    47941 cactgaaaaa tcaatgcttg ccatggatga caagaaatac acttttgaag tggacactcg
    48001 cgcaaacaaa actttagtaa aacaagcggt agaagctgct tttgacgtta aagtcaaaaa
    48061 cgtgaacatc gtgaacgtac gtgcgaaatt caaacgtatg ggcaaatatg caggatatac
    48121 aaaaaaacgt cgcaaagcga tcgttacatt gactgaagat tcaaaagaaa tccaattatt
    48181 cgaagctgct gaataagcac taatagataa ctcattaaat taggagggaa aaacgtggcg
    48241 attaaaaagt acaaacctac ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct
    48301 gaaatcacaa catcaacacc tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga
    48361 cgtaacaaca acggccgcat cactgtacgt catcaaggtg gcggacacaa acgccaatat
    48421 cgcgtgatcg acttcaaacg taacaaagat aacgttgttg caactgttca aacgatcgaa
    48481 tatgatccaa accgttcagc taacatcgcg ttagtacact atgaagatgg agtgaaagct
    48541 tacatcttag cacctaaagg actagaagtg ggtatgaaac ttgtttcagg tccagaagca
    48601 gatatcaaag tcggaaatgc tcttccatta gaaaacattc cagttggtac tgttatccac
    48661 aacattgaaa tgaaacctgg taaaggtgga caattgatcc gttctgctgg tacaagtgct
    48721 caagtacttg gtaaagaagg caaatacgta ttgatccgct tgaactctgg tgaagttcgt
    48781 atgatcttag ctgcatgccg tgctacaatc ggttctgtag gtaacgaaca acacgaatta
    48841 atcaacatcg gtaaagctgg acgctctcgt tggatgcgta aacgcccaac tgtacgtggt
    48901 agcgtaatga accctaatga tcacccacac ggtggtggtg aaggtaaagc tcctatcgga
    48961 cgtaaagcac cagtttctcc ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa
    49021 aaagctaaat cagacaaact tattgttcgt cgtcgcaaaa ataaataatt actttgacca
    49081 tgtgtcgcac attttgagag gaggttcacc atgggtcgta gtttaaagaa aggacctttt
    49141 gtcgatgagc atttaatgaa aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt
    49201 atcaaaactt ggtcacgccg ttcaacagtt ttcccaagtt ttgttggata taccatcgca
    49261 gtttatgatg gacgtaaaca tgtaccagtt tacatccaag aagacatggt aggacacaaa
    49321 ttaggtgaat ttgcacctac aagaacttat cgtggacatg ctgctgacga taagaaaacg
    49381 aaacgctaat ttgaggggag gataaatcca aatggcagaa caaatcacat cagctaaagc
    49441 aactgcaaaa acagttcgca cttcacctcg aaaagcccgt ttagtaattg accttatcag
    49501 aggtaaaagt gtcgcagatg caatttcaat cttgaaattc acaccaaaca aatctgctgg
    49561 aatcattgaa aaagtattga tgtcagcagt tgcgaatgca gaaaataact ttgacttaga
    49621 tgttgaaaac ttggtagtaa gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt
    49681 ccgtcctcgt gcaaaaggat cagcttctcc aatcaacaaa cgtacaagtc atatcacggt
    49741 agtcgtatca gaaaaataag gagggataat cagtgggtca aaaagtacat ccaattggaa
    49801 tgcgtgtagg catcatccgc gactgggatg ctaaatggta tgctgaaaaa gagtatgctg
    49861 agttcttaca cgaagattta agaatccgta aatttatttc aactaaactt gctgatgctg
    49921 ctgtatcaac agttgaaatc gaacgcgcaa caggtcgcgt aaacatttca attcacacag
    49981 ctaaaccagg tatggttatt ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat
    50041 taaacaaatt aactggtaaa agaatccata tcaatatcgt ggaaatcaaa aaaccagatc
    50101 tagatgcaaa attagtaggt gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc
    50161 gtcgcgctca aaaacaagcc atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa
    50221 ctcaagtatc tggtcgtcta aacggagcag atatcgctcg ttcagaagga tactcagaag
    50281 gaactgttcc tcttcatact ttacgtgcgg acattgatta cgcatgggaa gaagcagata
    50341 caacatacgg aaaactagga gttaaagtgt ggatctatcg tggagaaatt cttccaacta
    50401 aaaaaaacac tgagaaagga gggaaataat catgttagta cctaaacgtg taaaacaccg
    50461 tcgtgaattc cgcggaaaaa tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg
    50521 tgaatacggt ttgcaagctg ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc
    50581 tcgtatcgca atgactcgtt acatgaaacg tggtgggaaa gtatggatta aaattttccc
    50641 tcacaaatct tatactgcca aagcaattgg ggtacgtatg ggttctggta aaggggcacc
    50701 tgaaggatgg gttgcaccag taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc
    50761 tgaagaagta gctcgtgaag cgttacgtct agcttctcac aaattaccaa tgaaaactaa
    50821 gatcgtaaaa cgtgaggaaa tgggtggtga atcgaatgaa ggttaaagaa atcagagaat
    50881 taaccactgc cgaaatgctt gatcaagaaa aacaattaaa agaagaattg tttaatctta
    50941 gattccaatt agcaacaggt caattagaaa acactgcacg tattaaagaa gtacgtaaat
    51001 cgattgcacg catcaaaact gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca
    51061 ttaagcgtat gactgaagga agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag
    51121 acaaaatgga taaaacaatt accgttgctg tcgaaacaaa gaaaaaccac cctatctatg
    51181 gcaaacgcat gaactattct aagaaataca aagtgcatga tgaaaacaac acagcaaaag
    51241 ttggcgacat cgtgagaatt atggaaactc gtccattatc agctacaaaa cgtttccgtt
    51301 tgttggaagt agtcgaagaa gcagttatta tctaataaca cgagattttc ctatatagat
    51361 tgaatgcaaa atctgaaagg aggatacaca acgtgatcca agcagaaagt cgtttaaaag
    51421 tcgctgataa ctcaggcgca cgtgaaattt tgacgatcaa agtccttggt ggatctggtc
    51481 gtaaaactgc taacatcggt gatgtgatcg ttgctacggt taaacaagca acgccaggtg
    51541 gggttgtcaa aaaaggtgac gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc
    51601 gtcgtacaga cggttcatat attaaatttg atgaaaatgc tgcagtgatt attcgtgatg
    51661 ataaaagccc tcgtggaaca cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca
    51721 acttcatgaa gatcgtttca ctagcaccag aagttttata atcttgagac acttatcaaa
    51781 ggaggtgcga aacagttatg tttgttaaaa aaggcgataa agttaaagtt atcactggaa
    51841 aagacaaaaa caaagaaggc gttgtactag cagcgttccc gaaaaaagat aaagttattg
    51901 tcgaaggtgt aaatgtcgtt aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg
    51961 gaattgttga gatggaagcg ccaattcatg tttctaacgt gatggtggtt gactctactg
    52021 gtgtagctgg ccgagttggc tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa
    52081 aaaccggtga agttttagat aaataaaagt ttaaggaagg agggtcttac tgaatgaacc
    52141 gcctaaaaga aaaatatctt aaagaagtaa ctccatcatt gatggaaaaa tttaactata
    52201 gctctgttat gcaaacacct aaagttgaaa aaatcgtcat caacatgggt gttggtgatg
    52261 cggtatcaaa cgctaaaaac ttagataaag cagttgaaga attaactttg atcactggtc
    52321 aaaaaccaat gattacaaaa gctaaaaaat caatcgctgg attccgttta cgtgaaggaa
    52381 tgccaattgg tgcaaaagtt acattgcgcg gagaaagaat gtacgaattc ttagacaaat
    52441 tggtttctgt ttcactacca cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg
    52501 acggtcgtgg aaactatact ttaggtatta aagaacaatt gatcttccca gaagtagatt
    52561 atgatttagt agataaagta cgtggtatgg acatcgttat tgtaacaaca gcaaactctg
    52621 atgaagaatc tcgtgagttg ttgacacaat taggcatgcc attccaaaaa taattaaagg
    52681 aggcgaacta cgtggctaaa aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt
    52741 caacacaagc ttatactcgt tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt
    52801 tccatctttg ccgtatttgc ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga
    52861 agaaagctag ctggtaaaaa gtaaattcgc ataagaagga ggtaaaagat caatggtcat
    52921 gacagatcca attgcagatt ttctaactcg tatccgtaac gcaaacatgg ttaaacatga
    52981 gtctttagaa gtacctgcat caaaaattaa acgtgacatc gctgaaatct tgaaacgtga
    53041 aggtttcgtt cgtgatgttg aatacatcga agatgacaaa caaggcgtga tccgtgtttt
    53101 cctaaaattt ggtaaaaacg gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc
    53161 aggtttgcgt gcttatgtca aagctgacga agtaccaaaa gtattgaacg gtttaggtat
    53221 cgcaatcatt tcgacttctg aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat
    53281 cggcggcgaa gttatcgctt acgtatggta atcaagtaga atctacaagg aggtgtcttt
    53341 aagtgagccg tatcggaaat aaagtcgttg ttatccctga aggagtaaca gttactcaag
    53401 acggaaacaa cgttacagtt aaaggaccaa aaggtgaatt gactcgtact ttttcagcag
    53461 atatcaaaat gaatatcgaa ggaaacgaag taactttcac tcgtccaaac gacagtaaag
    53521 aaatgaaaac aattcatgga acaactcgtg cgaacttcaa caacatggtt gtaggtgtca
    53581 gcgaaggttt ccaaaaagcg ttagaactaa tcggggttgg gtaccgtgca caattacaag
    53641 gatcaaaact tgtattgaac gttggttact cacatccagt agaaatcaca ccaccagctg
    53701 gagtaactgt tgaagtacca gctaacacac aagtaattgt taaaggtgcg aacaaagaag
    53761 aagttggcga attagctgca aacattcgtg gaactcgtcc tccagaacct tataaaggta
    53821 aaggtattcg ttatgttggc gaatatgtac gccgtaaaga aggtaaaact ggtaaataat
    53881 cgtttagaaa aattgcatag aagccagcag caggaaaaag gcatttggct gaagtttacc
    53941 ttcaaaaaaa tacattttta cttgtgccgc agcttctatt tttcgaaacg tatttactgc
    54001 ttgaaatagt agtacaataa aaataaagag gtgacaattg tgattacaaa accagataag
    54061 aacaaaacac gtcaaaagag acatcgtcgt gtacgtaaca atatctctgg tactgctgag
    54121 cgcccacgct tgaacgtttt tcgttctaac aaaaacatct acgctcaaat tattgatgac
    54181 gtagcgggtg taacgctagc aagtgcctct accttggata aagaaatttc aggtggaaca
    54241 aaaacagaag cagcacaagc tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc
    54301 attaaagtag tagtctttga ccgtggtgga tacctttacc atggccgtgt gcaagcttta
    54361 gctgaagctg ctcgcgaaaa tggactagaa ttttagaaga aggaggaata ccattcatgg
    54421 tttatatcga tccaaaacat ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa
    54481 caaaagttgt taaaggtgga cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca
    54541 aaaacggaca cgtagggttt ggtacaggta aagctcaaga agttcctgaa gcaatccgta
    54601 aagcaattga agatgcgaag aaaaacttag ttgaagtacc tatggttggt tcaacaatcc
    54661 cacacgaagt aatcggtgct tttagtggtg gtagaatcct aatgaaacct gctgtagaag
    54721 gttctggggt tgctgctggt ggaccagttc gtgccgtatt agaattagct ggggtagcag
    54781 atatcacttc taaatcttta ggctcaaata caccaatcaa cgttgttcgc gcaacagttg
    54841 aaggtttgaa acaattgaaa cgtgcagaag aagttgcaga acttcgcggc aaatcagttg
    54901 aagaattaat cggataagga ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg
    54961 cagtattatc ggacgtcctc aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa
    55021 gattaacagt tcagtggtta agccttctaa tgaagcaatt aaaggcatga tcaatactgt
    55081 ttctcaccta gtggacgttg aagaaattta atttagaaaa atcataacta gaacattaag
    55141 gaggtgccaa acctatgaaa cttcatgaat taaaacctgc tgaaggttca cgccaagtac
    55201 gtaaccgtgt tggtcgtggt acttcatctg gtaacggaaa aactgccgga cgcggacaaa
    55261 aaggacaaaa agctcgttca ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac
    55321 cattgttccg tcgtttacca aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag
    55381 tcattaatct tgatgtattg aaccgctttg aagacggagc tgaagtaact cctgttgctt
    55441 tagttgaagc tggaatcgtg aaaaacgaaa aagctggaat caaagtatta gctaatgggg
    55501 aactaaacaa gaaattaact gtgaaagcag caaaattctc taaagcagct caagaagcaa
    55561 ttgaagcggc tggtggatca atcgaggtga tctaatgttt agactattaa aagatgcttt
    55621 taaagtaaaa gacattagat cgaaaatcct atttactgta tttgtgttgt ttgtatttcg
    55681 cctaggtgca catattactg taccaggcgt aaatgctgct tcattgcaaa acctttcttc
    55741 tttgccattt ttcaacatgt tgaacttggt aagtggtagt gcgatgcaaa acttttccat
    55801 tttctcaatg ggggtttcac catacattac tgcttctatc gtcattcagc ttcttcaaat
    55861 ggacatcgtg cctcgatttg ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact
    55921 gaatcaagcg actcgatatc tgacattagt attggcattt atccaatctg tcggtatcac
    55981 tgctggattc aatcaatacg ctcaattagg tttcgtgaat aatcctaacg tagcaactta
    56041 cgtaacgatc ggtttgattc taacagccgg tacgatgttt gttacttggc taggagaaca
    56101 gatcacagaa aaaggtatcg gaaatggcgt atcaatgatc atttttgcag gtatcatttc
    56161 tagattgcct gaaagtatca gagaaattta tgaagattac tttataaata ttgatccttc
    56221 agacatatgg aaatctgcta tttttgtggc cattttacta gtagcggtct tgttgatcgt
    56281 gacattcgta acatttttcc aacaagctga acgcaagatc ccgatccaat atacgaaacg
    56341 cgtagctggc gcgcctacaa gcagctatct gccgttgaaa gtaaatgcag ctggagttat
    56401 tccagttatc tttgctagtt cgtttattgc tacgccaaat gctgttttac aagcattgag
    56461 cggtagattc ggttctgaaa actggtacaa agtgatgatg gagatcttta actataatac
    56521 agcacctggt gctacgattt atacgattct gatcgttgcc ttcactttct tctacgcctt
    56581 tgttcaagta aaccctgaga aattagcgga aaacttgcaa aagcaaggaa gttatatacc
    56641 aagcgtacgc cctggtaagg ggactgaaga gtatgtctca agattactga tgcgtttaag
    56701 tacagttggt gcagtcttcc ttggtttagt ggcattgctt ccaatcattg ctcaaatggt
    56761 ttgggatctt ccacaatcaa tcggattagg cggtacaagc ttactgatcg ttatcggtgt
    56821 agctttagaa actgcgaaac agttagaagg cttaatgctg aaacgtaaat atgttggatt
    56881 cattgagtaa gaaagcgtgt tgaggtttcc cctcaacaca cgttctcaca catttattag
    56941 gagggaaaac aatgaacctc attttaatgg ggctgccagg cgcaggtaaa ggaacacaag
    57001 ctgaaaaaat cattgatgct tatggtattc cgcacatctc aacaggagat atgttccgcg
    57061 cagcgatgca aaacgaaaca gctcttggcc tggaagcaaa gtcttatatg gataaaggtg
    57121 cattagttcc tgatgaagta acaaatggaa tcgtgaaaga gcgactagct gaaccagata
    57181 cagagaaagg cttcttatta gatggctttc cacgtacgct agatcaagca aaagctctgg
    57241 acgcaatgct aaaagatttg aataaaaaaa ttgatgctgt catcgatatc catgttggtg
    57301 aagaaatctt ggtcgaacgt ctagccggcc gatttatctg cagcaattgt ggagcaactt
    57361 accataaaat tttcaaccct acaaaagttg aagacacatg tgatcgttgc ggtgggcatg
    57421 aattctatca aagagaagat gataaacctg agacggttaa aaatcgtctg gctatcaaca
    57481 tcaaaaatag tgaaccaatc ttggcttatt ataaagaaca aggtttgcta aacactattg
    57541 atggtgctcg tgaaatcgat gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc
    57601 acgattgccc gtagccgact tgcattatgc agttactctt gtcaagttca ataaattatg
    57661 atagaatatg tcagtccgac ttctagagaa tcctctagat gatccatttt gagaattttg
    57721 aggtgggaac tgcagtttcc gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag
    57781 gtactgtgcg tggcaaaaga agatatgatt gaagtcgaag gtacagtcgt cgaaactttg
    57841 ccgaatgcaa tgtttaaagt tgaactagaa aacggacacc aagttcttgc tactgtttca
    57901 ggtaaaattc gtatgcatta catccgtatc ttacctggtg acaaagtaac ggttgaattg
    57961 tctccatacg atcttaaccg tggccgtatt acttatcgct ttaaataatt gtactccgta
    58021 aaactacagg aggtattatc atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt
    58081 gtaaagtaat tcgtcgtaaa ggacgcgtta tggtgatttg cccagcaaat ccaaaacata
    58141 aacaacgtca aggataattg gaggtgctat aaatatggct cgtattgcag gagtagatat
    58201 tcctcgtgat aaacgtgtag tagtttcact tacttatatc tatggtattg gtaacactac
    58261 tgctaaaaag atcttagctg atgctggtgt atctgaagat gttcgcgttc gtgaattaac
    58321 gaatgaacaa acagatgcta tccgtgcaga agtagataaa ttaaaagttg aaggggatct
    58381 tcgtcgtgaa gtgaacttga acatcaaacg tttgatggaa atcggttctt accgtggaat
    58441 ccgtcatcgc cgtggattgc ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg
    58501 taaaggccca gctcgcgcta tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta
    58561 aaacttcatg gcagcaaaaa aagtgaatcg taaacgccgt gtgaagaaga atatagaagc
    58621 aggtattgca catatccatt ctacattcaa taacactatc atcatgatca ctgatactca
    58681 cggaaatgca ttagcgtggt catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc
    58741 aactcctttt gccgctcaaa tggcagcaga aactgctaca aaagcagcaa tggaacacgg
    58801 attaaagact gttgaagtaa cagttaaagg acctggttct ggacgtgaag cagcaattcg
    58861 ttcattacaa gctacaggtt tagaagtgac tgcaattcgt gacgtgactc cagttcctca
    58921 taatggtgcc cgccctccaa aacgccgtcg tgtttaatga gtgccgctca ttttgagttt
    58981 tatcagtaac gtcattgaac aagacacttt tcgttttgaa aggggtaaag ataagaatga
    59041 ttgagttcga aaaaccaaga atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca
    59101 tcgttgaacc tcttgaaaga gggtatggga ctactttagg gaattcccta cgtcgtattt
    59161 tattatcttc tttgccaggt gctgccatca ctaatattca aatcgatggt gtactgcatg
    59221 aattctctac catcaaaggt gttagagaag acgtcactca aatcatcttg aacattaaag
    59281 gcttagcgct gaaaatgtat ggccaagaag aaaaaaccct tgaaatcgat attaccggac
    59341 ctgctacagt aactgctggc gatattatcg ttgatagtga tgtagaaatt cttaacaaag
    59401 atatgtacat ctgtagtgtc tctgaaggag ctacgttcca tgctcgctta acagtgaaac
    59461 ctggacgtgg ttatgttcaa gcagatgaaa ataaaaaaga agatatgcca attggcgttc
    59521 ttccagtcga ttcaatctac acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac
    59581 gtgttggaca ccgtgaagat tttgataaat tgacgatgga gatttggaca gatggttcaa
    59641 ttgaaccgtt ggaagcaatg agtttgtctg cgaaaatcat gactgaacat ttggatattt
    59701 ttgtgaactt aactgatgaa gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac
    59761 aaaaagaaaa aatgttggaa atgacgatcg aagagttaga cttgtctgtt cgctcttaca
    59821 attgtttgaa acgtgcagga atcaatactg tacaagaact tacaaataaa tctgagcctg
    59881 aaatgatcaa agtccgtaac ttaggacgta aatcattaga agaagttaaa gcaaaattgc
    59941 acgacttagg tttaggttta cgtaaagaag attaaaacct tttacgaagg agggaaacca
    60001 acgtgagtta tcgtaaacta ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt
    60061 taacaactga tttattaatc aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag
    60121 ttcgctcaac tgcagaaaaa atgatcactt taggtaaacg cggagatttg catgcacgtc
    60181 gtcaagctgc ggctttcgta cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca
    60241 tcgtcattga atcagcttta caaaaactgt tcaacgatat tgcacctcgt tacgcagaac
    60301 gtcaaggcgg atacacacgc attttgaaga cagaaccaag acgcggtgat ggtgcaccaa
    60361 tggttgtctt agaacttgtc taaaatcaca ctaataacat cactttatag atgattcttt
    60421 tagagtgtta tgatgttgga agatattttt ccgagtctag ctcagcgctg ctcctcaagc
    60481 gaatatcgct tggggagtaa gcactcatca taaagtgttt ttttatacct tcaattacct
    60541 gtcatgacag atatgtttga aggcataaaa aaacatttta agcgagaaat aattgaaaat
    60601 tttgtctttt tttgatagtg ttagtaggaa ctttaggatg aagaaggagt aatcatggag
    60661 ccaattattg aattagaaaa gataaattat aaataccagc cagatgatct tcgccccgca
    60721 ttgaaagatg tctcctttac aattgataaa ggtgagtgga tcgctattat tggacacaat
    60781 ggttcaggaa aatcgacact tgccaaaacg atcaatggat tgcttctgcc agaatcaggg
    60841 atcgtgaaag tgggaaatca aatattggat gaagaaaata tttggacgat ccgacagatg
    60901 gttggaatga tttttcaaaa tccagacaat cagtttgttg gttcgacagt agaagatgac
    60961 gttgcttttg gtcttgaaaa tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa
    61021 gatgcacttg agaaagttag aatggctgag tttgcttcac gcgagcctgc tcgtttatca
    61081 ggagggcaaa aacaacgtgt tgccattgcc ggcgtggttg ccttacgacc tgatatcatt
    61141 attttagatg aagcaaccag tatgctagat ccagaaggtc gtgaagaagt gatctctacg
    61201 atcaagaaga tcaaagaaga aagtcaattg accgttatat ctatcactca tgatattgat
    61261 gaagcagcta atgccaatcg gatactagtg atgagacaag gcgaacttgt ccgtgaaggt
    61321 acaccaaaag aaattttttc tgctggtcct gaactgatcg acttaggtct agatctacca
    61381 tttcctgaaa aactgaagag cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat
    61441 atgacagaag aaaggatggt ggattggtta tggacatccg ttttgaacaa gtagacttca
    61501 cttatcagcc gaatacacca tttgaacaaa gagccttatt tgacatcaat atgacgatca
    61561 aagaaaacag ttatactgca ttagtagggc ataccggaag cgggaaatca accttacttc
    61621 agcatttgaa tgcgcttgtt aagccaacaa gcggtacagt ccatattgga gaacgagata
    61681 ttcagccgga tacggataat aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct
    61741 ttcagtttcc agaagcacag ctattcgaag aaacggtagc aaaagacatt gctttcggtc
    61801 ctaaaaactt tggtgtcagc gaagaagaag cactagtcct agcaaaagaa acattagaac
    61861 aagttgggct ggatgaaagc tatttggaac gttcgccatt tgaactttca ggagggcaaa
    61921 tgcgtagagt agcaatcgct ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg
    61981 aaccaacggc aggacttgat ccccagggca gaaaagaaat gatggagatg ttttggcgtc
    62041 ttcataagga acagcagatc actattgtat tagttacgca tttaatggat gatgtagcca
    62101 attttgcaga ttatgtctat gtgttggaaa agggacgaat cgttaatagc ggggagccgc
    62161 aagaggtctt tcaaaacatt gaatggctaa aagaaaaaca actaggcgta cctactgcca
    62221 ccgagtttgc agaagaatta atggctaaag ggatgaactt tgctaccttg cctttgacag
    62281 ctgaagaatt agcagacgct atcgtccatt atgctggagg aaatgcccat gatgaataaa
    62341 ttgatatttg gtcgttatat gcctggagat tccttgatcc atcgattgga tccccgagct
    62401 aaattattag ccagttttta ttttatcgga atcatttttt tagcaaataa ttgggaaacg
    62461 taccttttgt tggcattttt cacattactt tctgtcttct tgtcaaaagt agatttgaaa
    62521 ttttttatcc gaggcattcg cccattgatc tggctaatcc tcttcactgt agctttgcag
    62581 gtcttgttta caagtggcgg agaaacttat tggagttggg gtgttttcca tatcactgaa
    62641 tttggtgtga tgaacggcct ctttattttc tgtcgattcg tgttgatcat ttttatgtcg
    62701 actctactca cgcttactac accgcctttg gaattatcgg atgcgattga gtacttgctt
    62761 cgtccattga aagccgtacg gttcccagtc cacgaaattt cattgatgct gtcgattgct
    62821 cttcgttttg tgcctacatt gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct
    62881 cgcggtgttg attttggtga aggaaaccta gttcagaaaa tgaaagcagt cattcctttg
    62941 ctgattcctt tatttgtcag cagcttcaac cgtgcggaag atcttgcgac tgctatggaa
    63001 gcaagaggct acaaaggtgg ggaaggaaga acgaaatatc gtgtattgca ttggcataat
    63061 agagacacga tggttatcat tgcttttgta ttattaactg tgctactgat ctttttaaga
    63121 ggataaacgt tcagtgggcc gctatctatt cttttcttgc tgatctgttt gcaagaaagg
    63181 atgcaagcag tgtatgacaa gaaatattag ggtctgtggc agactaaggt cacaggcctc
    63241 tttcttttat ctgaaaagaa agaacgaaaa aattttatag aaaaatcatt attccagata
    63301 accttttagg aacaaaggag agaaaaacat ggtacgttac aaagcaatca ttgcgtacga
    63361 cggaacaaat tttaatggat tccaaaaaca gccaaatggg cgtactgttc aagaagaagt
    63421 agaaaaaaca ttacaaaaaa tggcaaatgg aaaagaaatc acggtctttg gttcgggaag
    63481 gacagatgcc ggagtacatg cgatcgggca agtaatccat tttgattatc cagaggagcg
    63541 tccgttagaa cgaatgcgtt tcgcgctaga tacccagtca ccagaggata ttgctgtaag
    63601 acaagtagaa attgtttcag aagacttcca cgcaagatat ttggtcaaag aaaagacgta
    63661 tcaatttcga gtggatatag ggaaaccacg cagtcctttt cgacgccatt atgcaagtta
    63721 ttttccttac ccacttgatt tggaaaaaat tcagcgggcg cttcctgatt tattagggac
    63781 tcacgatttc acctcttttt gtgcatcagg aagctcgata gaggataaaa tacgtacgat
    63841 ctacgaggct aagatggagg taaatgaaac aaaagacgaa ttgttgttta catttagggg
    63901 aaatggcttc ttgtataaga tgatacggat cttagtagga acgcttttga aaattggcaa
    63961 tggccgattg cctgaagata gtatcccaga tattattgcg aaaaaagatc gtaatgcagc
    64021 aggacctaca gcacaccctg aaggacttta tttgtatgaa gtgaaatatg aataagaggt
    64081 tatgatagaa gtggtcagct tcaagaaata agaaggtatt tacgaaaatt acttctcaaa
    64141 attttgcaaa atttcggctt attttcgaag aagctacttc tggaacaccg tttaagaaag
    64201 aggtcgtgag cctgtcattt aactctgagt cacaaagagc aatttcaaaa aacagcttct
    64261 catctttgat gaatatcgtg acttgtggac gaggcgttga taagtgaatc atctttttgt
    64321 tacctatttt ttctgtaacc aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg
    64381 acagtctgaa caggttccat cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg
    64441 tggttgttct tctattgctg gatcaatcgt gccataaaag gggcgattgc ggtcaaggta
    64501 ccagacataa agaataaaca atgcaataca taggacaaaa aggattgcac ctatcgtaaa
    64561 tccttcaggc aagtaactga aagtgacagt atgtttacct gctggaactt ttatcatcaa
    64621 gaatgcgtct ttgaaattgc tgaccttgac tggtttccca tcaattttag ctttccatcc
    64681 tttgtcataa ggaatagttg tcaccaacat ggaatcttca gcggtattta cagtcccaga
    64741 agcttttctg ccagtggctt ccatttcgac acctttttct ttgatctgat tcattgcggc
    64801 ttgataggct acaacatcta gaccaacgac tttaggctcc atgaaactta tttctttcgt
    64861 cccataaaaa cttgcagtaa atgtcactgt tgtctcttga ggataatagc caagatcata
    64921 gtattgacca gaaatgttga tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc
    64981 actagaactt tctaattgcg caaaattggt tgggaacaaa cttaagtaag cttgtgtatt
    65041 tgcaggcaca tggacttccc aagtgagctc ttttggctgg ttatctgctg tttctgagta
    65101 agtgacgcca gtcgttgtct ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt
    65161 atagaatgta aaatactgtt catttagacc agacaatgta ttgatcaagt ttgtctgact
    65221 ggtcaaatta tctgtcactg gctgctcaac atcattaacg gacaacggtg cagtaaatgc
    65281 tagtggcaat gcattgacat tctgatacag tgtatagtcg ccgctttgac cagcttggcg
    65341 gaagccatat ttactgaacg tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa
    65401 cccatccatc agcaaagtat tattgttata acggatattc aaatttgtcc ctcttgaacg
    65461 gaaacctaat ttatccaggt aagaggaaga attacgattt cttatagaag aaaacaaact
    65521 gataccactg taaccatagt tgatcgaatc gtttgcagat actggatcca gattttccaa
    65581 gcggtaaaac gagtcggaat ctttttttgt ttcatttaca agagctttca agtcaggata
    65641 aggatcagta tataaactgc gggaagcata attccaatca tcaagaatgc catggatcat
    65701 actgttcgta ttgaccagtg cttcagcaga catgatcaat aataacaata tactcagatg
    65761 attgaatgaa agttttttat gctgataaaa tcccacagca aaagcataaa tccctaagaa
    65821 aaccactgtc aatacaaagg aagtcatcgt cacataggtg tagcttcctt caggtttgat
    65881 gccccatgcc accgcaaaga gagcagctac gaccaaaatg attcctaaca acagcccacc
    65941 atcgtcttgt tctatctttt cccatccttt tcctgcaagc aggagcacta agaaagagaa
    66001 cagataggca taacggaata aaaacatatt cggtgcatgc ataccatgcc agaacaaatt
    66061 caatggaaca agataaaaac ttgcgataag tatggcgaaa agactgccgt acaaaaattt
    66121 atttttcaac ggtactttcc gactggcaaa ataaaacaga cagaggacta atggtagcag
    66181 acctgcgtaa acgaacggaa tcgatccgta ttttgtcgtg tcataaacac caatcatatt
    66241 tttcatgatt agatccagat aagaagtcgc ttctgttttc aacgtagtga tctgactcag
    66301 tgcctcgcca tttgaccgaa gatccagcac tgctggcaat acaatgatca ttgaagcgcc
    66361 gcctgccagt aaagaagtga taccataagg aacaatcgtt tttttatatt ctgaccagtt
    66421 tgtcaataga cgggcgataa aatataagaa agaaaatacg ccaatcataa aacccatata
    66481 aaaactggat aaaaacaaga gtaaataact gataaataaa agtttcggcc gtttaagatc
    66541 cataacacga tgaatcccca aaatcactaa tggaagataa acaaatgcat ccagccacat
    66601 gatgatctcc gaatgtgcag tgataaatga gatcaatgcg taactgacgc taagtgcaac
    66661 atgcccccag cgaggaatct tgaacgtacg tgcaagaaac caaaaagcca atccggcaca
    66721 gccgacctta agcaaagtca gaacatacag tgcatctggc atcatctggt ttggaaaaaa
    66781 gaacaccagt ggcgtgaaga tcccgcctag atagtaagaa attaaagata aataattcaa
    66841 tcctagagaa gcattccatg tataaaaaat actttgcttt ccaagaagaa catttcggaa
    66901 gctggcatga aaattagaaa actgtgaaaa ggcatcactt gccaaaatac ttctgctgct
    66961 tccaggatag attccgatac ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa
    67021 actagctgct gcgtagcgtc cgttttctct aaagaatgac tttaaatttt ttttcatatt
    67081 tattttccac caaatttcta tgaagttgta ttcagtttat catatccttg tttttctctg
    67141 cttaaaaaga gtctagtttt cacaaaaaaa taaaatttag aataaataac ttcataattt
    67201 gttcgtataa caaaaataaa cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa
    67261 atttttatta aataaaaaac cgttcactat ctgaacggtc caagtatcat tcaattaaac
    67321 gcgttcaact tttcctgatt tcaaagcacg agttgacacc caaacttttt taggttttcc
    67381 gtcgaccata acgcgaactt tttgtaagtt aggtttaaca gtacgttttg ttgagttcat
    67441 agcgtgtgaa cggttgtttc cactttttgt cttgcgacct gtaaagtaac aaacttttgc
    67501 catggtaatt tcctcctttg ccttgatttg gagcaataga ataattcagc tcataccaga
    67561 ctaacatatc atatagaatg ggaatttgca agtacatttt catagataca attgacaatt
    67621 gagaagaata attgtaaatt aaacaaggtt atacctgaaa ttttgcaagg tttttgagta
    67681 ctttgggtaa ctgcctttta tttcaagttg cgctatgata aaatatgata gaagaaaaat
    67741 gggagttttt tacccgttaa ggaggctcat tcatggctgt aaaaattcaa acgtcagctg
    67801 gaaccattga aattaccaat gatgtaattg caacagttgt tggtggcgcc gcgacagatg
    67861 tgttcggtgt tgtaggaatg gccagcaaaa atcaaattaa agataatatt actgagattt
    67921 tgcgtaaaga aaattactca agaggagtag ttgtccgcca agaagataat ggtattgctg
    67981 tagatgttta tacgattatc agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc
    68041 aagaaaaagt gaaatacaat ctagaggcat tattaggcgt aactgccaat tcagttaatg
    68101 tctttgtaca aggtgttcgt gtactgccag actaggcagg agactggctg gtaactcagc
    68161 agtcgatctg aaggaggatt atctaggtga gtgtaacaga aataagtgca agccagttcc
    68221 aggaaatggt ccaggcaggt gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat
    68281 taaatgtatt ccctgtgcca gatggcgata cgggaacaaa tatgaatcta tctatgacta
    68341 gtggagctaa ggcagtagta gattcaacct cagaaaaagt aggagaatta gcagcaatgc
    68401 tttcaaaagg attgctgatg ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt
    68461 ttcgtggatt ttctaagcaa attccagatg ttgtctcatt aaatgcttct gatcttgctg
    68521 cagccttcac tcatggagtt gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa
    68581 cgattttgac agtagctaga gaagcagcga aagctggaga aaagaaagca aaaagtactg
    68641 atgatgtcgt tgaagtaatg acagctgttg taaaaggagc aaagcgtgct ttagctaaaa
    68701 ctccagactt gcttccagtc ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac
    68761 tgctgtttgt ttatgagggc tttttaagtg cattgaatgg agaattccaa gcggatgaaa
    68821 catatgaacc ttctccagca gaaatggatg atatggtcaa tgcagaacat catcgcagtg
    68881 tccaaggtca aatggcgaca gaagatatcc attttggtta ctgtacagaa atcatggtaa
    68941 aaatcggaga aggacctaca gtcgatagta cattcgatta cgaagaattc cgtaactatt
    69001 tagatggtat aggagattct ttacttgtgg tcaatgatga cgagatcatc aaagtccacg
    69061 tgcatacaga aaacccagga gaagtcttga attatggaca aaaattcggt tcattagtca
    69121 aaatcaaagt cgacaacatg cgcttgcagc atgagacgat tttggaacac gatgaagaag
    69181 tgaacgagtt tgcgaaaaaa gaaataccac gtatcccgta tggtatcgta gcgatcgctg
    69241 ctggtaaagg cgtccaagag ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg
    69301 gacaaacaat gaatccaagt acggaagaca ttgttaaagc gattcaagaa gtaaacgcag
    69361 ataaagtgat tgtcttgcca aacaataaga acatcttcat ggcagctgat caagcagcag
    69421 aagtagcaga tatcccagta gcagttgttc cttcaagaac gatttcccaa ggattgacag
    69481 ctatgctttc attcaatgat caagcttctt tagaagaaaa caaagaaacg atgacagaaa
    69541 tgttgtctag cgttgtgagc ggtcaagtca ctcacgcgat cagagatacc tcaattgacg
    69601 gtgtagagat caaagaagga gactttttgg gcatgatcga tggaaaaatc ctgatctcta
    69661 acccagatat cacagcaaca gcgttaagta cactagaaca aatgatcaat gaggacacag
    69721 agatcgtaac gatcttggca ggtgatgaag gaacagttga acaagcaaat gaatttgctg
    69781 agaaactaac agaaaaacat cctgatttgg aaatcgagat ccaccaagga gatcagcctg
    69841 tctatcctta tctattttct gctgaataat ttctgtgtca gaatatcttt ctaacacagt
    69901 attcattcat aaaaggcctt cggttagtga tcaggtatga tcgctgacca agtccttttt
    69961 ttgttaaagg agggaggaaa aatttatgcg ttctttagat gatccaatca ctatgctggc
    70021 aggagtcgga gaaaaaagag cagctagtct tgccagcttg ggcatccaaa cgattgaaga
    70081 cttactgact tattatcctt ttcgctacga agatattcag gaaagaaatc taaacgagat
    70141 ccaggatcaa gaaaaggtta ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg
    70201 atttggttat aaaaaaagcc gattgcaatt tcgtatgatg caagaacatg atgtgttcaa
    70261 cgtttctttt tttaatcaac cttatctaaa agaaaaagtc attttatcgg aggaaatcgc
    70321 cgtatacggt aaatgggatg caaagcgtaa agccttgaac gggatgaaga ttttaggttc
    70381 tcaaacaatg gatgactttt cgccgatata tcacgtcaac aaatcaattc gtcaaacgac
    70441 attagttgat ctaatcaaaa aagggtttca agaatacggc acattgattg aagaaaatct
    70501 gccaaacaac cttgtggaga aatatcgact gcttgaccga gcttctgctg taagaagtat
    70561 gcattttcca aaagaccacg aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga
    70621 gttcttttta tttcaaatgc gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa
    70681 tggtttggag atcctttatg atgtccaaaa attgaaacaa tttactcaaa agctaccttt
    70741 tgaactgaca aatgcacaaa aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc
    70801 gcagcatatg cagcgacttt tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc
    70861 gattgcgtta tacgctaccg ttacagcagg tttccaaggt gcgttgatgg tgcctacaga
    70921 aatcttggca caacaacata tggaaagctt gcaacagctg tttgatccca atgaagtaac
    70981 catcgcattg attacggggt caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact
    71041 agagcaaggg gaaattaacg tcgtgatcgg cacacatgca ttgatccaag atggagtcga
    71101 atttcagaat ttaggtctag ttattacgga tgagcagcac cgttttggcg tgaaccagcg
    71161 gaaagtactt cgtgaaaaag gctggcgacc agatgtctta tttatgacag cgactccgat
    71221 tcctagaaca ttagctatta cagcatatgg tgagatggat gtatctatca ttgatgaact
    71281 tccagctgga cgtatcccga tcgaaacacg ctgggtaaga acacctcaat tggacagtgt
    71341 actggattgg acgaaaaaag aactggcaag aggacatcaa atgtatgtca tctgtccgct
    71401 gattgaagaa tcagaagcat tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg
    71461 tgaactattt gcaccaaaat atgaagttgg cttgttacat ggtaaaatga agaatcagga
    71521 aaaagaagta atcatggaag ccttcaaaga caaccagatg cagatattag tttcgactac
    71581 tgtaattgaa gtaggggtaa acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga
    71641 tcgatttgga ttggcacagc tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc
    71701 ttcctactgt atcttagttg ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat
    71761 catgacagag accaccaatg gttttgtact aagcgaaaaa gatctggaat tacgtggtcc
    71821 tggtgaagta tttggcttcc gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt
    71881 gacggatgga aatgttttgg aggtagccag agaagaggct gcagcccttt ggcaaataaa
    71941 ggattggcag atgcttcctg agtatcagcc tttagcgcaa agcctccaaa tagaaaatca
    72001 ggataatcgt tatttcgatt agcagactgt gctatacttt atactgatga cggaaaaagg
    72061 agtggaaaca atgaaaattg ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat
    72121 tgtagaaggc gtggctttag cgcagcaaga ttttccagaa gtagaattct tactttacgg
    72181 aaaagaagca gaaatcaaga aatacttaac aaatgaaaaa aacatcacga ttatccatac
    72241 agacgaaaaa atcaatagtg atgatgagcc agtaaaagca attcgacgaa aaaagactgc
    72301 atcaatggtc cttgctgcac aagcagtgaa aaacggtgaa gcagatgctg tattttcagc
    72361 agggaataca ggcgcgctac tagcagcagg gttgttcatc gtaggacgta tcaaaaatat
    72421 cgaacgccct ggcttgatgt ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga
    72481 tatgctggat cttggcgcta acgcagaaaa caaaccagaa catttgttgc aatacggtat
    72541 tttaggttct ttttatgcac gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt
    72601 aaacaacgga acagaagcaa caaaaggtag tgaagtgacg aaaaaagcct atgaactcct
    72661 tcagaatgaa acaagcttga attttattgg aaacgtagaa gctcgtgagc tactcaatgg
    72721 tgtggctgat gtagtggtca cagatggatt tacaggcaac gctgttttga aatctattga
    72781 aggaacagca atgaatatga tgaatctatt aaaatctgct attctaaatg aagggatcaa
    72841 aggaaaaatg ggcgctatgc ttttaaaaga cggcttacgt tctttgaaag cagaaatgga
    72901 ttattccaaa catggcggtg ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca
    72961 tggagcaaca ggccccgatg ctgttcgcta tacgatccgc caaatccaca caatgcttga
    73021 gacagatgta gttggacaat tagtcgaaca atttgaaaaa agcgaagaat agtatttgga
    73081 tatctgaagc gatccgttcc attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa
    73141 attagccatg gtgtaggtaa ttgacaagtg tatgattctt ggaggtgaaa aagttgactc
    73201 gcgaagaagt atttaacaaa gtagcgaaaa ttatttcaaa tcattttgag atagataccg
    73261 ataaagtgac ggatgaattg aacatcaaag atgatttaaa agctgattca attagtatta
    73321 tggaattcgt tttagagctt gaagatgaat tcggtacgga aatttcagat gaagacgctg
    73381 aacaaattga aacagtcggt ggcgcagtag attatatcag cagccatctt aaataatttc
    73441 aaataaaagc aaagaaggat
//
LOCUS       DACDTO010000005        71380 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDTO010000005 DACDTO010000000
VERSION     DACDTO010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.005"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..455
                     /locus_tag="IWK70_000338"
     CDS             66..455
                     /locus_tag="IWK70_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase"
                     /protein_id="HAP9612915.1"
                     /translation="MSPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIA
                     VENNHEDFYTKADTKTYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTI
                     AFCLTGITAWNTFVELKKKRRRTQKFL"
     gene            583..1266
                     /locus_tag="IWK70_000339"
     CDS             583..1266
                     /locus_tag="IWK70_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9612916.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1575..1648
                     /locus_tag="IWK70_000340"
     tRNA            1575..1648
                     /locus_tag="IWK70_000340"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1609..1611,aa:Arg,seq:tct)
     gene            1807..2166
                     /locus_tag="IWK70_000341"
     CDS             1807..2166
                     /locus_tag="IWK70_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9612917.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2289..3920
                     /locus_tag="IWK70_000342"
     CDS             2289..3920
                     /locus_tag="IWK70_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9612918.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(3982..4623)
                     /locus_tag="IWK70_000343"
     CDS             complement(3982..4623)
                     /locus_tag="IWK70_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9612919.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            4905..5213
                     /gene="rplU"
                     /locus_tag="IWK70_000344"
     CDS             4905..5213
                     /gene="rplU"
                     /locus_tag="IWK70_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9612920.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5240..5584
                     /locus_tag="IWK70_000345"
     CDS             5240..5584
                     /locus_tag="IWK70_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9612921.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5597..5890
                     /gene="rpmA"
                     /locus_tag="IWK70_000346"
     CDS             5597..5890
                     /gene="rpmA"
                     /locus_tag="IWK70_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9612922.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6297..9131
                     /locus_tag="IWK70_000347"
     CDS             6297..9131
                     /locus_tag="IWK70_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9612923.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            9322..9813
                     /locus_tag="IWK70_000348"
     CDS             9322..9813
                     /locus_tag="IWK70_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9612924.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            9916..10905
                     /gene="manX"
                     /locus_tag="IWK70_000349"
     CDS             9916..10905
                     /gene="manX"
                     /locus_tag="IWK70_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9612925.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            10938..11741
                     /locus_tag="IWK70_000350"
     CDS             10938..11741
                     /locus_tag="IWK70_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9612926.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            11762..12676
                     /locus_tag="IWK70_000351"
     CDS             11762..12676
                     /locus_tag="IWK70_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9612927.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            12759..13133
                     /locus_tag="IWK70_000352"
     CDS             12759..13133
                     /locus_tag="IWK70_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9612928.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13272..13517
                     /locus_tag="IWK70_000353"
     CDS             13272..13517
                     /locus_tag="IWK70_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9612929.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13689..14753
                     /locus_tag="IWK70_000354"
     CDS             13689..14753
                     /locus_tag="IWK70_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9612930.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            14999..15424
                     /locus_tag="IWK70_000355"
     CDS             14999..15424
                     /locus_tag="IWK70_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9612931.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15417..15866
                     /gene="nusB"
                     /locus_tag="IWK70_000356"
     CDS             15417..15866
                     /gene="nusB"
                     /locus_tag="IWK70_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9612932.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            15963..16817
                     /locus_tag="IWK70_000357"
     CDS             15963..16817
                     /locus_tag="IWK70_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9612933.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            16818..18155
                     /locus_tag="IWK70_000358"
     CDS             16818..18155
                     /locus_tag="IWK70_000358"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9612934.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18160..18384
                     /locus_tag="IWK70_000359"
     CDS             18160..18384
                     /locus_tag="IWK70_000359"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9612935.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18386..19276
                     /locus_tag="IWK70_000360"
     CDS             18386..19276
                     /locus_tag="IWK70_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9612936.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19285..20103
                     /locus_tag="IWK70_000361"
     CDS             19285..20103
                     /locus_tag="IWK70_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9612937.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20210..20659
                     /locus_tag="IWK70_000362"
     CDS             20210..20659
                     /locus_tag="IWK70_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9612938.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20677..22359
                     /gene="recN"
                     /locus_tag="IWK70_000363"
     CDS             20677..22359
                     /gene="recN"
                     /locus_tag="IWK70_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9612939.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22414..22530)
                     /locus_tag="IWK70_000364"
     CDS             complement(22414..22530)
                     /locus_tag="IWK70_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9612940.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22603..23559)
                     /locus_tag="IWK70_000365"
     CDS             complement(22603..23559)
                     /locus_tag="IWK70_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9612941.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23730..24098
                     /locus_tag="IWK70_000366"
     CDS             23730..24098
                     /locus_tag="IWK70_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9612942.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24268..24699
                     /gene="mraZ"
                     /locus_tag="IWK70_000367"
     CDS             24268..24699
                     /gene="mraZ"
                     /locus_tag="IWK70_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9612943.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24720..25679
                     /gene="rsmH"
                     /locus_tag="IWK70_000368"
     CDS             24720..25679
                     /gene="rsmH"
                     /locus_tag="IWK70_000368"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9612944.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25684..26085
                     /gene="ftsL"
                     /locus_tag="IWK70_000369"
     CDS             25684..26085
                     /gene="ftsL"
                     /locus_tag="IWK70_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9612945.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26086..28278
                     /locus_tag="IWK70_000370"
     CDS             26086..28278
                     /locus_tag="IWK70_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9612946.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28304..29266
                     /locus_tag="IWK70_000371"
     CDS             28304..29266
                     /locus_tag="IWK70_000371"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9612947.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29280..30641
                     /locus_tag="IWK70_000372"
     CDS             29280..30641
                     /locus_tag="IWK70_000372"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9612948.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30667..31755
                     /gene="murG"
                     /locus_tag="IWK70_000373"
     CDS             30667..31755
                     /gene="murG"
                     /locus_tag="IWK70_000373"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9612949.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            31770..32990
                     /locus_tag="IWK70_000374"
     CDS             31770..32990
                     /locus_tag="IWK70_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9612950.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33146..34471
                     /gene="ftsA"
                     /locus_tag="IWK70_000375"
     CDS             33146..34471
                     /gene="ftsA"
                     /locus_tag="IWK70_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9612951.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34494..35735
                     /gene="ftsZ"
                     /locus_tag="IWK70_000376"
     CDS             34494..35735
                     /gene="ftsZ"
                     /locus_tag="IWK70_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9612952.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            35752..36429
                     /locus_tag="IWK70_000377"
     CDS             35752..36429
                     /locus_tag="IWK70_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9612953.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36443..37042
                     /locus_tag="IWK70_000378"
     CDS             36443..37042
                     /locus_tag="IWK70_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9612954.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37057..37344
                     /locus_tag="IWK70_000379"
     CDS             37057..37344
                     /locus_tag="IWK70_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9612955.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37393..38175
                     /locus_tag="IWK70_000380"
     CDS             37393..38175
                     /locus_tag="IWK70_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9612956.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38196..38903
                     /locus_tag="IWK70_000381"
     CDS             38196..38903
                     /locus_tag="IWK70_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9612957.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39165..41963
                     /gene="ileS"
                     /locus_tag="IWK70_000382"
     CDS             39165..41963
                     /gene="ileS"
                     /locus_tag="IWK70_000382"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9612958.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42012..43538)
                     /locus_tag="IWK70_000383"
     CDS             complement(42012..43538)
                     /locus_tag="IWK70_000383"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9612959.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43553..44200)
                     /locus_tag="IWK70_000384"
     CDS             complement(43553..44200)
                     /locus_tag="IWK70_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9612960.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44384..44713)
                     /locus_tag="IWK70_000385"
     CDS             complement(44384..44713)
                     /locus_tag="IWK70_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9612961.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            44890..45618
                     /gene="sfsA"
                     /locus_tag="IWK70_000386"
     CDS             44890..45618
                     /gene="sfsA"
                     /locus_tag="IWK70_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9612962.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45634..46647
                     /locus_tag="IWK70_000387"
     CDS             45634..46647
                     /locus_tag="IWK70_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9612963.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46647..47924
                     /locus_tag="IWK70_000388"
     CDS             46647..47924
                     /locus_tag="IWK70_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9612964.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            47987..50689
                     /locus_tag="IWK70_000389"
     CDS             47987..50689
                     /locus_tag="IWK70_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9612965.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            50841..51158
                     /locus_tag="IWK70_000390"
     CDS             50841..51158
                     /locus_tag="IWK70_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9612966.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51188..51508
                     /locus_tag="IWK70_000391"
     CDS             51188..51508
                     /locus_tag="IWK70_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9612967.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51616..53076
                     /locus_tag="IWK70_000392"
     CDS             51616..53076
                     /locus_tag="IWK70_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9612968.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53144..53365
                     /locus_tag="IWK70_000393"
     CDS             53144..53365
                     /locus_tag="IWK70_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612969.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53396..53578
                     /locus_tag="IWK70_000394"
     CDS             53396..53578
                     /locus_tag="IWK70_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9612970.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53578..53991
                     /locus_tag="IWK70_000395"
     CDS             53578..53991
                     /locus_tag="IWK70_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9612971.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54114..55295
                     /locus_tag="IWK70_000396"
     CDS             54114..55295
                     /locus_tag="IWK70_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9612972.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            55684..55757
                     /locus_tag="IWK70_000397"
     tRNA            55684..55757
                     /locus_tag="IWK70_000397"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55718..55720,aa:Arg,seq:cct)
     gene            complement(55794..55994)
                     /locus_tag="IWK70_000398"
     CDS             complement(55794..55994)
                     /locus_tag="IWK70_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9612973.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56299..57531)
                     /locus_tag="IWK70_000399"
     CDS             complement(56299..57531)
                     /locus_tag="IWK70_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9612974.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            57785..58354
                     /locus_tag="IWK70_000400"
     CDS             57785..58354
                     /locus_tag="IWK70_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9612975.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58532..58972)
                     /locus_tag="IWK70_000401"
     CDS             complement(58532..58972)
                     /locus_tag="IWK70_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9612976.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59130..59894
                     /gene="map"
                     /locus_tag="IWK70_000402"
     CDS             59130..59894
                     /gene="map"
                     /locus_tag="IWK70_000402"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9612977.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            59926..60849
                     /locus_tag="IWK70_000403"
     CDS             59926..60849
                     /locus_tag="IWK70_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9612978.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            60925..62064
                     /locus_tag="IWK70_000404"
     CDS             60925..62064
                     /locus_tag="IWK70_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9612979.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62057..62857
                     /locus_tag="IWK70_000405"
     CDS             62057..62857
                     /locus_tag="IWK70_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9612980.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            62857..63684
                     /locus_tag="IWK70_000406"
     CDS             62857..63684
                     /locus_tag="IWK70_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9612981.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63662..64396
                     /locus_tag="IWK70_000407"
     CDS             63662..64396
                     /locus_tag="IWK70_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9612982.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64496..65362
                     /gene="rfbA"
                     /locus_tag="IWK70_000408"
     CDS             64496..65362
                     /gene="rfbA"
                     /locus_tag="IWK70_000408"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9612983.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65376..65948
                     /gene="rfbC"
                     /locus_tag="IWK70_000409"
     CDS             65376..65948
                     /gene="rfbC"
                     /locus_tag="IWK70_000409"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9612984.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            65970..66998
                     /gene="rfbB"
                     /locus_tag="IWK70_000410"
     CDS             65970..66998
                     /gene="rfbB"
                     /locus_tag="IWK70_000410"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9612985.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67096..67947
                     /gene="rfbD"
                     /locus_tag="IWK70_000411"
     CDS             67096..67947
                     /gene="rfbD"
                     /locus_tag="IWK70_000411"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9612986.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            67982..70015
                     /locus_tag="IWK70_000412"
     CDS             67982..70015
                     /locus_tag="IWK70_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612987.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70059..71339
                     /locus_tag="IWK70_000413"
     CDS             70059..71339
                     /locus_tag="IWK70_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9612988.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT        23182 a        12133 c        15301 g        20764 t
ORIGIN      
        1 ttcttctcgt cgtcttggtt ccccgaattt ttggtttgac tccttatatt attgaggaca
       61 aagaaatgag tccatattat gcacaaggaa atctgatata tgtacgaccg aaaaatacaa
      121 atgagatttt agtcggagat attattacat attatgaaaa ttcaggaaaa aaaatctcga
      181 cgcgtcgcgt aatagccgtt gaaaacaacc acgaagattt ttatacaaaa gctgatacaa
      241 agacttatat tgagcctgga gtagtaaaaa agagaaatat tatcggtagt cccatctttc
      301 agattccata tattggacta gtcctttcaa aaacggcatt tgcatggatt caggggttat
      361 tctttacgat tgctttttgc ctaacaggaa tcacagcatg gaataccttt gtagagttga
      421 aaaagaagcg gagacgaaca cagaaatttt tgtaagaatg ttaaaaacat aaatgtattc
      481 cagagatcca acgactcttc tgaacatgaa acacgagaaa acactcatgc agcttgtaga
      541 tttagtcagt tgttcgtata atgtgtgaag aggaggagta gtatgcgcaa tgaaatgatg
      601 catcgtcctg tagtcaaaga agaactattg gattttatgc gtacccagca aaaaaaatta
      661 ccgggtgagc taggcaaact ggaagaagaa gctcatgtag cagaagtacc agttattccc
      721 catgaaacag ttgtattttt gaaattttta cttggacagc ttcagcctga acgaatcttg
      781 gagatcggag cagctatcgg tttttcatca agtgtcatgg ccacgacaat cggagaaaac
      841 gctcatgtta cgacaattga tcgtttcgat gtcatgatcg agaaagctaa aaaaaactat
      901 gaacgtctag gactgacaga taaagtgact ttgcttgaag gtcaagcagc agatatcttg
      961 ccggagttgt ctggtccgta tgactttatt tttatggata gtgcaaaatc aaaatatatt
     1021 gaatttttac ctgaatgctt acgtttgtta agaaaaggtg gcgttttgat ggtagatgac
     1081 atcttccaag gaggaacgat cttgctccca gatgaagaga ttccccgtgg caaacgagct
     1141 atccatcgta aattaaatga atttcttcgt gttgtgatgg atcatcccga tttgacatcg
     1201 acaatcttgc cattaggtga tggtgtgatc ttaatgacaa aggaatcaga aacaatcgag
     1261 ttgtgataca tgattatggc agcctgtaga taatcgattg aatcgagggc tcaattcgaa
     1321 cttgatgtaa aagtttctat atttgtaagt caattaaagg atacaggaaa tctaaataaa
     1381 caggagtagc atgaagtcaa tgtagctcct gttttcaatt gataacctta gaacagctga
     1441 aggagaatgt ctcgttttaa aagattgatt tagtagttgg gccaaaataa ttcattattt
     1501 gctaattaaa taactatttc gtattgacag aagcaattta attatctata attattgatg
     1561 ttccgctgat tcttgtcata gtagctcagc tggatagagc atccgccttc taagcggacg
     1621 gtcgggggtt cgaatccctc ctgtgacgct aaaaaaccgt tagaaactga ggtttctaac
     1681 ggtttttctt tgttttttgt gccaatattg tttgttttca aaggcaaata aaataaaaaa
     1741 acatccttat cgagtacaat tataaggagc aaaaaaataa aactcatcag aaaagaggaa
     1801 gaagatatga cgatactatc tagaatttta gtgactctag ttgcaattga atttttttat
     1861 atcatgtata tcgaaacggt gagaactgat tctgacacga caagtagggt ttttaaaatg
     1921 agtaaagaag agttaagcag aaaatctgtt caaacattgt ttaagaacca aggagtgtac
     1981 aatggactat taggagtggg actactttat ggagcttatc tttcttccgc atctaaagaa
     2041 atcacaagta tgctattaat atctattttt tttgttgcat tatatggcag tttgactagc
     2101 gatcgttgga ttttactcaa acaaggtggg ctagctctgt tggcattgct ttctattttc
     2161 ttataaatat agaaaaaata agaatttatc taacaggaaa ctttgtgaaa aatattactt
     2221 tatctcatga aatatgatat tctaatgaat ggtattttag aaaatttcat gaaaaaggag
     2281 ttagataggt gttcaaaaat tatgtttcct cactaaaaaa agaattttca ggttatgatc
     2341 tttcaagatt tcagaaagat cttttggctg ggatcacagt agctgctgta gcattgcctt
     2401 tagccttggc ttttggggta tcaagcggag ctgatgcggc cgcaggtttg attacggcta
     2461 tcattgccgg tatcgttatt ggcggtttat caggcggttt ttatcaaata tcaggtccga
     2521 caggtgcgat ggcagctatc ttgatgtcta ttgcggcaac aaagggaatg caaggtgtat
     2581 tgcttgctac ttttttagcg ggtctatttt tacttgcagc aggacttttc cgattaggaa
     2641 cactgacttc ttttatccca gcacccgtaa ttacggggtt tacttcaggt attgctatca
     2701 ttattgcatt aggtcagatc gataatttat ttggtgttca ctcggaaggt acaaacgtta
     2761 ttgaaaaact aagtagttat caaacgatag gatttccgat aaacacagcc tctttgatca
     2821 tgggagggct agtcatttta gggatgttta tctatccaaa aaaatgggcg aaacgaatgc
     2881 ctagttcgct gctggctatc attttggcaa cagcagttat gctattgttc catttaccag
     2941 tagcaactgt gggtaagatt ccacaaacat tgatcagtag taatcgtttg aatatgggag
     3001 attttagctt ttctgccttg caacaagtgg cagttccagc tattagcatt gctttattag
     3061 gaatgattga aagcttgcta tgtggtgctt cggctggccg aatggcaaat aagcccttag
     3121 atagcaatca agaattaatg gcgcaaggaa ttggaaatct gttgcttcct ttctttggcg
     3181 ggattcctgc tactgcggcc atcgcccgta caagcgtggc catcaaatct ggtgcacaaa
     3241 ctagattagc tggtatgata catgcagttg tcttactgct ctccatgctt gtttttgcgc
     3301 cagtgatgtc acagattcca atgccagcac ttgcaggtgt tttgattgtg acggcttggc
     3361 gtatgaatga atgggaaacg atcaaggagc tatttactaa taaatattgg tcagcagttc
     3421 tattgtttat ccttacgatg atttgtacag ttatctttga tttaagtatt gctatcgtga
     3481 tcggggtgat cggcggctgt gtattttttg ttgtcaaaag cgctgctatc acgatttcat
     3541 tagaaacgat cgattggcaa agaatggggc tgccagaaac ggaaaagctg aataactgga
     3601 cagtagtgta tattagtggt ccactgtttt ttatgtcttc agaaaaactc aaaaataaat
     3661 tggaagaact aaatcatcac gatggtgtca ttttctccat gcgaggcgta ccaagtattg
     3721 atttgacggc acgtgcagtt tttgatgaat ttcaagaaaa agcagagcaa agaggacaaa
     3781 cgattatcta tacttccttg cagccagaag tagaaaaaca actttctcac ttgtgggaac
     3841 atacattcac gcatcaacat caaacagttg ctcatgcgtt gacagcttta catgcgaaat
     3901 atactgcaat tagccattaa aacaatgaaa aagaacagac aagactgtaa actgaagatt
     3961 ttatggtctt gtctgttctt tttaatattg ctgtaaaagt gaagctagta cttgccagtc
     4021 ttgatggata acaggtacaa gggagcgatt gtagaaaata gctgctggat gaaaaagtgg
     4081 aagaattcga tagttttttt ctgaccattc gtaggcatcc ttttgttctg ttaattgtaa
     4141 gacaggccct gtatagagtt gaccatgatt tttggtaacc gtatattctt tgccaagtaa
     4201 acgttgaaga ccaatgtttc caagtgtcgc aattaatttt ggctggacct tcatcagttc
     4261 gtaatccaat attggtgcat gcgcaaatac ttcggaacga gtaggtgtcc gatttggata
     4321 aacagtttct gactgttgtg tccgtttatt gatccgatga gtcacgcgat acgggcggct
     4381 ccttacagca ctggtgatat acacctcttc tcgtgttaag ccagttgaag aaagtgcctg
     4441 catcaattct tttccagctt gaccgctaaa aggaacatgc gtatcaatct ctgttttccc
     4501 tggggcttct cccacgagca tcagtttagg atgaagaggt ccttgcccag cgataaatcc
     4561 ttctacagga cggccgttca ttcttaaaga agcttcttgt atcagttctt ttgggtattc
     4621 catttatgct cctccttttt cttttattat aagaataaaa gaaacacaat gcgactattt
     4681 tatttggtag aactgtgagg atagaaaagc agaagtattg cttgaatttt tgttttactt
     4741 tttgatttta aatttagttg actaacggtt gctgttctgg taagctagta ctgttgtaat
     4801 tgtggactcc acagctacaa ccgctcagaa caagtaataa gcacagcata tgctgtcact
     4861 tcggatggcg agtctaagta tatcagaagg aggtgcttta tagcatgtac gcaattatta
     4921 aaactggtgg taaacaagta aaagttgaag tgggacaagc aatctatgtt gaaaaacttg
     4981 acgttgaagc aggagaaaaa gttgtttttg acgaagttat cttagtaggc ggagaatcta
     5041 caaaagtagg cgctccaact gttgcaggtg caactgttga aggtactgtt gaaaaacacg
     5101 gcaaacaaaa gaaagtcgtt actttcaaat acaaacctaa aaaacattca caccgtaaac
     5161 aaggtcatcg tcaaccatat acaaaagtgg ttatcgacgc gatcaacgct taatagcgtt
     5221 tgagaaagaa ggcctatata tgatcaaagg aacatttaag cgaaatgacg caggtcagat
     5281 cgtttcattc actttgaccg ggcatgctga tgctggtcct tatggaagtg atatcgtttg
     5341 tgcgggtgtt tctgcgttag cgatcagcac tgtcaacggg attgcctcat tagccggttt
     5401 tgagccgatt gttgaaatga atgaagaaga aggcggttat ctttataccg aagtcacttc
     5461 aggaatgaca caagaacaaa acaatattgc tgagattcta ttagaaaatc ttttattagg
     5521 attacaatcg atagaagctg aaaactcaga atatatccaa attaaaacaa ttaatgataa
     5581 ataggaggtg cagaccatgt tgttatctat gaatctacaa ttatttgccc acaaaaaagg
     5641 tggcggttct acatctaacg gccgtgactc agaatcaaaa cgtctaggtg ctaaacgtgc
     5701 tgatggacaa acagttacag gtggatcaat tctttaccgt caacgcggta caaaaatcta
     5761 tccaggtgta aacgtgggta tcggtggaga cgacacttta tttgcaaaag ttgatggcgt
     5821 agtacgtttc gaacgtaaag gccgtaacaa aaaacaagtg tctgtttatc cagtagctca
     5881 agaagcttaa ttgaatctta tgattaaaga ggttgtgaca agacttttgt cacagccttt
     5941 tttgtttata aaaataaaag ttgtagtgga ggctacgaag tttactttgt ctttttacac
     6001 ttaatatttt tttgccaatt tggaatcagg atgttttgat aactatcgtt ttcaattgat
     6061 ggaagctata ttgtttgcta tcagtgaaat aattatatgg taaacagtgt tctttttctt
     6121 gtttccatta taaaaatcgt atcgggattt ttcgttcttt taaattgaac atttctatct
     6181 atccgcgtgt tatcaacaaa acaaaatcag gtaaatagtt gacgaaacag aaataaatac
     6241 atagtattat gttttgaagc ctaagtacaa tagtctatag aaaaggagcg ataccattgg
     6301 caaaacgcat tgaccgaatt tatatgtatg tcaaagaaaa aaccgctcat ttaacaaagg
     6361 cagaatatga tcaaggagta acaacacagg aaattgctga agtgttaggg attcagcgaa
     6421 caaatagcag taaagatctg aatcagctag tgcgcgaagg aaaactgata aagacagacg
     6481 gacgaccagt acgttacata tatcaaacac tcgttacgca cgtaaagcca gttacaaaac
     6541 atgtagtcag ttacaaagaa acgccggtct ctgaagaaaa aaatacactc caaatagata
     6601 ctaaggatat ctttgcaaaa atcattggag ctaacggcag catgaaaaat tcagtggaac
     6661 aggcaaaagc tgcgattttg tatccgccaa gaggattaaa ttgtttgatt acaggaccta
     6721 ccggatcagg aaagacttat tttgcacacg cgatgtatca ttttgcaaaa gcgcatcatg
     6781 taatagatga ggaaacggaa ctgattgttt tcaattgtgc tgattatgcc aataaccctg
     6841 agcttttgat gagtcattta tttgggtacg caaaaggtgc ttttacaggc gcagatgaag
     6901 aaaaaatggg gattatcgat caagcggatg gcggtatgct gtttttggat gagattcacc
     6961 ggttgccgcc tgaaggtcaa gaaatgatct tttactttat ggatcatgga acttacagtc
     7021 gtcttgggga aacaacgaaa tcccatgaag caaatgtacg gatcgtaggt gcgaccacag
     7081 aagacccagg ttcttctcta ttagaaactt ttgttcggcg tatcccaatc aatatcaaac
     7141 ttccggcttt tgaaaaacgt cctgcaaatg agaaagtcga tttagtaaaa atcatgattg
     7201 cccacgaagc aaaccgcacg cagcgaaaga tttctttgac agaagatgta gcaaaagcat
     7261 tgatcggcag tgtgacctat ggaaatatag gacaattgaa gtcaaatatc cagttagtat
     7321 gtgcacgagg atttctaaat catatgcaat cacccgaaat ctcgatcaca atcgatgatt
     7381 tgacagaagg gattcgtagc ggattgattc aattagcaag caaccgcagt gcaatggcag
     7441 agctttctaa aatattggag ccaaagataa ctgtagcacc aaatgatacg atcatgaaaa
     7501 tccaatcgga ctcctatgag ttaccttata atctatatga tatcatcgga gataaagcag
     7561 ctttactgaa gtcggatggt ttggatcaag aagcaatcaa tcatttcatt tctacagata
     7621 tcaacatcca tctgaagtct ttctataaag accatggttt ttcttttaat gcagacagca
     7681 aattagcaga atttgtagat ccgaaaatca tagaagtgac caatcagatt tatgtaatgg
     7741 tcaaaagatc tttaccatat gagtttcagc aaaactttat ttatgctatg agcttgcata
     7801 tcagttcttt tttgaaaaaa atcaacttag gagaagaacg gcatacgaat gataacatcc
     7861 gtgagatggc ttttgatttt ccagatgaat atgctgttgc gaaagaagtg cgtagttata
     7921 tcgaaacgta ttttcaagtg gagattccag aaagtgaaga ctattacttg accgttctac
     7981 tcgtctcgtt acgggcaaat caagcaagcg gcagaatcgg tgtagtcgtt gcagctcacg
     8041 gaaacagcac agcaagcagt atggtccaag ttgtccaaca gttgttagat gcagagaacg
     8101 tacgagcagt agacatgcct ttggatatgg atccgaaaac agcattggac cgaattgaac
     8161 gcaatgtaca agaagtagat gaaggaagcg gggtcatact cttagtagat atggggtccc
     8221 ttgcgtcgtt taattcgcaa attcaaagag acactggaat tccagtacgt actgttgata
     8281 tggtaacaac ttctctagtt ttggaaactg tacgaaaggt ctcagtatta gggacagatc
     8341 tagatatgtt gcatgaatca ttgaaaaatt ttcgaggtta cgcagaaata tcttctgtag
     8401 aatctgttca ggaagcaaaa cctattcata agaaaaaagc tgttttggct atctgcgcat
     8461 ctggcgaagg tacagcacaa agaatcaaag aattgatcga acgagcggta aacaagcgtc
     8521 aagaaacaga attagaagtt ctagctttgt cgattgtgga agtgaaagaa gaactgccag
     8581 ttatccaaaa agaatatcag atcatagcta ctactgggat tactgatttg aatatccaag
     8641 ccccatttat tcctttagaa cgttttattg atcaaaatat cgaagtcatt ttggatcagt
     8701 tgctgatgga atcagaattg gaggaaactg aattcatctc cctagatgaa gagtcagcta
     8761 aaaatacgtg tgttgagttt atctctgata acttcatctt tattaatggt agtaagttaa
     8821 tcgatccaat gtggcaattt tctacacaaa tctctcagac gacgggaatc ggcgatgaag
     8881 aatatggatt caaaatcaac ctagtgatgc acacagcagg aatgatcgaa cgtattattc
     8941 ggaatgaacc attgactgtg gaagaaaacg aattgacaaa tacaacgaat gatcctcttt
     9001 atagccaatt ggcagcctca gttgttctgt tggaagatca gataaaagta aaagtgccga
     9061 tagaagaaat gtattatctg ctacgtctag tccataatca gttagataaa aaagaataca
     9121 ctgtgccatg aatacacaaa aacgatacac ttttatagtg tatcgttttt gtgtattggt
     9181 taagaatcct ttaaattcaa ttagtgtatc gtaaaaaagt tggcacgctt tttgcttata
     9241 gtagtgtgtg ctaggaggtg gttgttcaca cagaaatgat aggggtttct gtgaagtagt
     9301 gaggaagaag gagttttgca gatggatatt cgattggcac gtatagatga tcgtttaatc
     9361 cacgggcaag tcgcaacagt ttggtcgaag caaacaggtg ttgaacgtat cattgtgatc
     9421 agcgatgtag tcgctcaaga taaattgaga aagtttctac taaaagaagc tgcaccgccg
     9481 gggatcacag caaatgtaat aaccgtgcag aaaatgattc aaatctatca tagtggatta
     9541 ttatcacgag caaaagtgat gcttttattc accaatcccc aagatgttga agcggttgtc
     9601 cgctgcggtg tacagttgaa gtcgttgaat atcggcggta tgagttttac tgaaggcaaa
     9661 tcaatgatta ccaattttgt ttcagtaaat caacaagata ttgattcctt tcgttacttg
     9721 aacagtcaag aagtggagtt agagattcga aaagtacctg ctgacaggaa aatccttttg
     9781 atggaactac tagcgaaaat caaaaaggtg tagcaagaat ggttgttgga aaaggtcaaa
     9841 taattctgtt gtaatggtta gcaattatta atatttttga atttgttaca acaaaagtaa
     9901 ataggaggta taaatatggt agggattatc ctagcaagtc atggacaatt tgctgaagga
     9961 atcttacaat ccggttcaat gatctttggc gaacaagaaa acgtcaaagc cgtgattttg
    10021 aaaccaagtg aaggtccaga tgacttgaga gccaaattgg aagaagcggt tgcttctttt
    10081 gataatcaag acgaagtgtt attcttagtt gatttatggg gcggaacacc gttcaatcaa
    10141 tcaaacacat tattcgaaga gcacaaagat aaatgggcaa tcgtcagcgg tttgaattta
    10201 ccaatgttga ttgaagccta cgcatcacgc ttttcaatgg aatctgctca tgaaattgca
    10261 gcacacatca ttgaaacagc aaaagacggt gtaaaagtaa aaccggaaga attagaaccg
    10321 gcagaagcac cgaaagcagc agttgaagac gcacaaccta aaggagcact tccagaagga
    10381 actgtggtcg gagacggaaa aatcaaatat gtattagcac gtgttgactc acgtctgtta
    10441 catggacaag ttgcgacagc ttggacaaaa gcagtgcaac caaaccgtat tatcgttgtt
    10501 tcagatgcag tatcaaagga tgatctgcgt aagagactaa tcgaacaagc tgctcctcca
    10561 ggtgtaaaag caaatgttat tcctattagc aaaatgatcg aagtagcaaa agaccctcgt
    10621 tttggaaata ccaaagcatt gcttttattt gaaaatcctg aagacgtatt gacagctgtt
    10681 gaaggtggcg tagacatcaa agaattaaat gttgggtcaa tggcgcattc ggtcggtaaa
    10741 gtcgttgtaa gtaaagtatt gtctatggga caagaagacg ttgaagcgtt tgagaagctt
    10801 gaacaaaaag gtgtgaaatt cgatgtacgt aaagtaccta atgattcacg cgataacatg
    10861 gatgatattt tgaaaaaagc aaaagctgaa ttagcaaaag cgtaataaat aatattaaaa
    10921 tttataggag gcttatcatg tctattattt caataatttt agtcgtatta gttgcctttc
    10981 tagctggtat ggaaggtatt cttgatgaat ttcaatttca tcaaccacta gtggcatgta
    11041 cattgatcgg tttagttacc ggtaacctcg aagctggtat cgtacttggc ggtacgcttc
    11101 agatgatcgc tttaggttgg gcaaatatcg gtgccgctgt agcaccagat gctgcattag
    11161 catcagtagc atcagcaatt attttagttt taggtggaca aggtgttaaa ggtgttcctt
    11221 ctgcgatcgc gattgccgtt cctttggctg ttgcagggtt gttcttaaca atgatcgtac
    11281 gtacaatcgc tgttccaatc gttcatttga tggatgcggc tgctgaagaa ggaaacatcc
    11341 gtaaggttga aatgtggcac atcattgctg tatgtttaca aggggttcgt atcgcggtcc
    11401 cagcagcagc gttgctattt atcccagcac aaactgtgca atcattcctt gaatcaatgc
    11461 cagcatggtt aacagatggt atggctatcg gtgggggaat ggtcgtagct gtaggttacg
    11521 ctttggtaat caacatgatg gctacaaaag aagtatggcc attcttcgtt attggtttcg
    11581 ttgtagctgc aatttctcaa ttaacattga ttgctctagg tgctttaggt gttgcattag
    11641 ctcttattta cttgaacctt tctaaaatgg gtggttcatc aaataatgga ggcggaagca
    11701 actctggtga ccctttaggc gacattttga atgactatta atcttgaagg aggaaaataa
    11761 aatggcagaa gaaaaaatca aattatcaaa aaaagatcgt ttagctgttg cgtggcgctc
    11821 taccttcatt caaggttcat ggaactacga acgtatgcaa aacggtggct gggcattttc
    11881 tatgattcca gcaatcaaaa aattatacaa aacaaaagaa gaacgttctg cagcattaaa
    11941 acgtcacttg gaattcttta atacacaccc atacattgct tctccaattt taggggttac
    12001 attggctctg gaagaagaac gtgcgaatgg cgcgcctgtt gatgatgtag cgattcaagg
    12061 ggttaaagta ggtatgatgg gtcctttagc tggtgttggt gacccagtgt tctggtttac
    12121 agtacgtcca atgctaggtg cactaggtgc ttcattagct atgggaggaa atatccttgg
    12181 accaatcatc ttcttcttag gttggaacat tattcgttgg gcattcatgt ggtatacaca
    12241 agaatttggc tacaaagctg gttctaaaat tactgatgac ctttctggcg gattgttaca
    12301 agacgtaaca aaaggtgctt caattttagg gatgttcgta ttagctgccc ttgtacaaag
    12361 atgggtatca atcaaattct taccagttgt atcaactgta aaattagata aaggtgctta
    12421 catcgaatgg gataaattgc cttcaggtgg tgaaggaatc aagagtgcat ttgaacaagt
    12481 taataatggc ttagcccttt ctcctgaaaa ggtaacaaca ttacaagaca acttagacca
    12541 attgattcct ggtttagctg cattaggatt aacattgtta tgtatgtggt tgcttaagaa
    12601 aaaggtttct ccaatcgtta tcatccttgg tttattcgta gtcggtgttg ttggacacgt
    12661 catcggactt ttataatcca taaaaatata gctcgggcgt tgtcccgagc tttttttata
    12721 tagtatactg ttcataaaca aatatctgga gggagaaaat ggttcaatca atcaatacaa
    12781 aagtagactt ggtaattgat gctacttcgt ttacgggtct gacagattac gggcaaatca
    12841 tgataggtga taatggtttt gagttttacc atgcgcgaga tagacgcaaa tatatccaaa
    12901 ttccatggga agaagtggat tatgtactag catcagtcat gctaaaggga aaatggattc
    12961 ctcgatatgc cattcaaacg aaaaaaaatg gtacatacac attttcttcc aaagaaccta
    13021 aaaaagtgct tcgtgcgatc agagaatatg tagatgaaga tagaatggta caatcattga
    13081 gtttctttga tgtagtgaaa cgtggtattc gtagtatgtt taaacgcaag taaaaaataa
    13141 agaaaacttc taagaggagg ttttctttat ttttttactt tgcagaagaa aatatgaaat
    13201 agcaaaacca attatttgtc ccctttaaat cccatatgct aaaattgatt agcaaagaag
    13261 gaggttatat tatgagttgg ttatggacat tgattgttgg agccgtgatc ggagcaattg
    13321 caggtgcgat caccagtaaa ggaaattcga tgggctgtgt aacgaatatc attgctggac
    13381 tagtcggttc aagtattgga caagcgattt tcggcgaatg gggaccaaaa gctgcaggta
    13441 tggcacttat tccatctatt ttaggcgcag tgattttggt agcagttgtc tcatttttct
    13501 ttggaaaacg atcataagtt tttacaaaat agtaaactaa aaggagttgc agcgagactg
    13561 tgactccttt ttggctttat ttatttaaaa taaaaagaag ttttccggaa atttttattt
    13621 aactgtcgtg tacgaagaaa agtttgctat aatggaaaaa agcgtgcaaa gaaagaggga
    13681 gtttcattat gatgctacgt gtagaaaaat tgcgcaaaaa aatgcaagaa gaaaatttgg
    13741 attcattttt ggttaccagt ccgtacaatc ttcgttactt aacgaacttt acaggtacga
    13801 caggattagc tgttattact ttggaaaaag cattttttat tactgatttt cgttataccg
    13861 agcaggcggc agcgcaggct caagggtttg aaattattaa aaatgtcgga cctatttttg
    13921 atgaggtagc aaacttagta cgcaaagaag aactgcactc attagctttt gaggaaacaa
    13981 cagtctcatt tttagaatat tcggttttag aagaaatcat cgatgcggaa ttagtacctg
    14041 tttcacagat gatcgaagaa ctcagagaag tgaaagatga agaagaaatc gctattatcg
    14101 aaaaagcatg tcatattgcg gatatggcat atgatcacat cctgaaaatg atccaaccgg
    14161 gcatgactga gattgaagtc gcaaatcaac tagactttta tatgcgttct cttggcgctt
    14221 caggtgtttc ttttgatacg atcgttgcta gcggattgcg ttcggctatg ccgcatggtg
    14281 tagcaagcaa aaaaatcatc gaacaaggtg acttgatcac attggacttt ggttgctatt
    14341 acgaagggta cgtttccgat atgacacgta cattcgctat tggtgatcct ggggaaaaat
    14401 taaaagaaat ttatcaaata gtcttagatg ctcaattagc agttattgat gctgcaaaac
    14461 caggaatgac tggtgtccaa ttagatgcag ttgctagaga ttatatttca aaacatggtt
    14521 acggggaagc atttggacat tctacaggac atgggatcgg tttagagatc cacgaaggac
    14581 caaacgtttc ttctcgtgca gaaaagcaat ttgtaccagg aaatgtgatc actgatgaac
    14641 cagggatcta cttaccagga atcggtggag tacgtattga ggatgacttg ttgatcacag
    14701 aagatggaaa tcgtgtgttg actcgttcac caaaagaatt gatcatctta tagtttcatc
    14761 aatagaagaa gtaagcgtaa aaatcagtgt tcacagactt tgttatagaa aatcactcga
    14821 tttaatgata cactatagaa gaacttttaa gctgtaagag ataataaagt tgggtgtttt
    14881 ctccaattca tagcagtttg cagctgatcg gcggcagttt tcttaccacg tgatcactat
    14941 gccgcatcat agagaattcg gatgtatctc gtgcttaggc aaagggagga ataaataaat
    15001 ggcagatgaa aaaaatttga ttttaaacgc aaaccaagat ttaggagaga tcgtgatcgc
    15061 tcctgaagtc atcgaagtaa ttatcgggat tgcagcttct aaagtagaag gcgtatacgg
    15121 aatgcgcgga acttttgcca gcaatgtcac tgaattatta ggacgtgcag ctcacggaaa
    15181 aggtgtttac ttagcaaacg aggaagatgg cttgaaagta gatctttact gttatctaga
    15241 atatggtgtt tcagttccta aagtagcaat ggaaatgcag gaacgggtca gccagcaagt
    15301 ccttttcatg acggatatcg atttatctga agtcaatatc catgtagtag ctgttgttcc
    15361 tgaaaaatta gaacaaccaa caatcgaaga actttttgaa gacgaagagg aagagaatga
    15421 ataaagaatt atcaagacac gagattcggg aaatggcatt gcaggcttta tttccattag
    15481 acttcaatgc agacctaaca aaagaagatg cgattttcaa cgcaatcgaa ttagaccacc
    15541 gcgacatgat caacgaagat gaatcagagt ttgttcctgt ttatcttgat acgctagttg
    15601 gcggtgtctg tgcaaaaaaa gatgaattgg ataaagtgat tgaaaaacat ttaaaaaaca
    15661 attggcacat caatcggatc tcaaaaatgg atttggtgat cttgcgtatc gcgatttttg
    15721 aaatgactta tgtgacagat gtaccagcgg ctgttgcttt aaatgaagca atcgagttgg
    15781 caaaaacatt tagtgatgac cgttcacgta aattcgtcaa cggtgtttta tccaatgtat
    15841 tgaaagaatt agaagctggg gcgtaagtcc tagctttttt ctatgaatca tcataaacct
    15901 gttataaatc gggaaaattc tatggtagaa tgaaaacaca aaaaatatct gggagtggtt
    15961 agatggcaga attgattaac ggaaaagaat tagctgaaaa aatgcaggca gagattgctg
    16021 aaaaaatcca aaaattgaaa gataatggta tccatcccgg attagtcgtt ttattggtag
    16081 gggaaaatcc agccagccaa atctatgtaa gaaataaaga acgtgtagca aaagaaatcg
    16141 gaatccattc attggtagag cgttatcctg agacgatttc tgaagacgaa ctacttgcag
    16201 aaattgaaaa atacaatcaa gatcctagtt ttcatggtat attggtccaa ttaccattgc
    16261 caaagcatat agatgaagaa aagatcctgt tagctatccg ccctgaaaaa gacgtagacg
    16321 gttttcatcc gatgaattta ggcagactcc ttgcgggtaa accggacaag attccttgta
    16381 ctccatacgg catcatgaaa atgtttgaag cttattctat cgatccggcg ggtaaacgag
    16441 cagtagttat cggacgaagc aatatcgtcg gtaaaccgat ggctcagcta ctcttgatgg
    16501 cagatgcaac tgttactgtt gctcattcta aaaccgaaaa tctggctgaa ctggcacaag
    16561 aagcagatat tttagtcgta gcaataggac gtggacattt tgtcacaaaa gaattcgtaa
    16621 aaccaggtgc tgtagtgata gatgtaggga tgaatcgtga tgctaacggt aagctgatcg
    16681 gtgatgtgaa atttgatgaa gtggagccta ttgcaggctg gatcactcct gtgccaaagg
    16741 gtgtagggcc aatgactatc acgatgctga tgtatcaaac cgtaaaatct gccgaagctt
    16801 ttggagcagg tgaatagatg gcacaagaat atttaactgt cactacatta acgaagtatt
    16861 tgaaaagaaa atttgatgcc gatccttatt tagaacgcgt atatttaaca ggcgaaatat
    16921 cgaatttccg tttacgtgcc aatgcccatc aatatttcag cttaaaagat gaccatgcaa
    16981 aaatatctgc tattatgttc aaaggagcat tccaaaaatt acgattccag ccaaaagaag
    17041 gaatgaaagt ccttgtcgtt gggcgtattt cattatatga agcgggaggt tcttatcaaa
    17101 tctatgtaga gcacatggag cctgatggaa taggtgcttt atatcaagaa ttggaagaac
    17161 gaaaagaaaa gctgatgaag gaaggccttt ttcaagggcc taaaaaaatg ctgccacgtt
    17221 tcccaaagcg aatcgcggtc cttaccagtc caagcggagc ggttatccgc gatatcatca
    17281 cgacggtgaa aagaagatat ccaattgtcc aattggtttt gttcccaaca gttgtacaag
    17341 gaaaccaagc agcggacaat gttgtcagga atatccagag agtagaagag atgggcaatt
    17401 tcgatacaat gatcatcggt cgtgggggtg gttcgattga ggatttatgg ccgtttaatg
    17461 aagagcgggt tgctcgggcg atttatcaag ctaatacgcc ggtgatttca tctgtaggac
    17521 atgaaacaga taccacgatc gctgatttag tagcagatgt tcgggctgcc actccaacag
    17581 cagctgcaga attagctgtt cctgttttgt cagaagagtt gatgcgcatt caagaacggc
    17641 aagctagatt gcagcaggcg tttgtacgcc agatccagcg caaacaagaa cggttcgagc
    17701 gtgctcgacg ttcctatgta tttcggcagc ctgaaagatt gtatgaaggt cagaccatca
    17761 aattagacca gttgaaacag cgattatatc aagctacaca gcaaatttat catgcgaaag
    17821 aaaaacaaac gatcgcatta agccaccaat tggaacaagt ggctccgctt tatcgagtta
    17881 aagcagctcg ccaagaaact gcttatctag aaaaacgctt agaagagaaa atccaacaat
    17941 atatgaaaca acagcagcag cgttttgctc aagctgttca atctcttgat ttgttgagtc
    18001 cattgaaaat catgggaaga ggctacagct atacgacgtt aaatgatcaa gtagtaaaaa
    18061 gtgtcaccga gattcatcaa ggggatttgc tgacgatcca ttatcaagat ggaatcgtta
    18121 aaggacaagt acaagagata gacaaaggag aataaaaata tggaaaatcc aacttttgaa
    18181 gagtcactgc aagaattaga aaaaattgtc atgcagttag aacaaggaga cgttccatta
    18241 gaatcagctc tagattcgtt taaacgagga atggaattaa gcaaacattg ccaagataca
    18301 ttagctaaag cagaagagac cttgacgaaa atgatgacag aatcaaatga agaagttgat
    18361 tttgacggaa acgaggaaag ctgagatgag attaactgac ttttcagcta ttcatctacc
    18421 tgctgtagaa aaagaaatct tgtctttttt agatgagcat acaacggata gaggactcta
    18481 tgatgcgatg agttattcag tgaaagcagg cgggaaaaga atacgtccct tattactttt
    18541 aacggccgtt gcttcctttg atgagcctat tgatgttccc gtctatcaag tagcagctgc
    18601 tttagaaatg gtgcatacgt attccttgat ccacgatgat ttgcctgcga tggacaatga
    18661 tgatcttcgt agaggaaaac ctaccaatca taaagtattc ggcgaagcat tagctatatt
    18721 ggccggagat ggtttgctta caggagcatt ccagctaatc agtatggcac acttaggtaa
    18781 ttctcctaag cttctgctgc ttcagcaatt agctgtttgt gcgggatcac aaggtatggt
    18841 tgctgggcaa gctgcagata tcgagggcga atcgaaaaag ctttctttag aagaattagc
    18901 atttattcat gaaagaaaga cagggcattt gattcgttat gccttgctgg ctggtggcat
    18961 attagcgaag caacctgaag aaatcttatt gctacttcaa cgattagccg aacatttagg
    19021 tttagctttt cagataagag atgatctact cgatgtgatt ggtacaacga aaacattagg
    19081 gaaaacagct ggtaaagatg aacgtatgga aaaaaatact tacccgcgtt tattaggcct
    19141 agaaaagaca cgtgaggcgt tggagattga gctgttatcc gccaacaaga tcatagacaa
    19201 attagaagag gacgtttttt cttttgatgg cgggctgatg cgccagatga tcaagcaatt
    19261 tgatgtggag aaatgaggga aagaatggaa aaagaacgcg tagatgtatt agcggtcaaa
    19321 caaggattat ttgaaacaag agaacaagcc aaacgttcag tgatggctgg tttgatttac
    19381 aacgaaaaaa acgagcggtt tgataaaccg ggagaaaaaa ttccagtgag tagtgaatta
    19441 agagtcaaag gtaaaaaact tccttatgtt tctcgagggg gattaaaatt agaaaaagca
    19501 ctcaaagcat ttgatctatc tgttgaagga aaaacattgt tagatattgg tgcatccact
    19561 ggcggattta ctgatgcagc tttgcaaaat ggcgcaaaaa tgagttatgc tttagacgtt
    19621 ggctacaatc agctagcttg gaaaataaga caagatccta gagtcgtcgt aatggaaaga
    19681 gtgaattttc gttatgcaaa acccgaagac tttaccttag gaattccaga agttgctgtt
    19741 atcgatgttt cttttatttc actaaaactg atgctgcctc cattacatgc tattttaaaa
    19801 gaagagggag aagtgattgc tttaatcaaa ccccaattcg aagcaggcag agaagctgta
    19861 gggaaaaacg gaattgttcg tgaccctcag acccatcaaa aagttttaga agacattttg
    19921 tcatttgcgg cacaaggtgg atatgatgtg ctggaattaa gttattctcc gatcacgggg
    19981 ggagaaggaa atatcgagtt cttggctcac ttgaggaaag tacccgaaag cggaacaatc
    20041 aattcagcta ttaatatggc agaagtggtt tcaaatgcac acgaacagtt tgaccataaa
    20101 taaattgtaa ctgtgaaaat caagtgagaa gtatgcggat tcttctttat tataccaagg
    20161 aaataagcta tcattgatta agaagaatat gctaaggagc gaagacgtaa tgagaaaaaa
    20221 agatcggcat cgtttgatta ctcgattgtt gagtgagcaa gatatccgaa agcaggaaga
    20281 atttgttgaa atcctgaaaa gcaaaggaat atctgttact caagctacga tttccagaga
    20341 tataaaggaa ctgaaactga tcaaggtacc ggcttcagat ggcggctacc gttatagcat
    20401 accagctgaa acaaacgaag atgtaaatac taaattggaa aaactattaa aagacgcttt
    20461 cgtggcagtt gaccaaatgg aaaaatttgt tattttgaaa acattgccgg gaaatgcttc
    20521 ggcagcagca aatttgattg ataaacgtta caagaaagaa ttattttcga ttatcaatga
    20581 tgacgataat gttttgatga ttacacgaac tgaagaccag gcagttttat tgaagaaaga
    20641 cttcctgcat tacctttaag ttgaggagcg aatgtgatgc tacaagaaat cagtattact
    20701 aactttgcga ttattccgga attgcggctt tcttttcacg aaggaatgac cgcgctgacc
    20761 ggagagactg gtgcgggtaa atccattatt atcgatgctt taggtctgtt agcaggagga
    20821 agaggttcaa gcgattatat acgtcaagga gcagaaaaat gtgtgcttga agggttgttt
    20881 gaactaccaa aacaagaagg attctctgaa ctgatggttg aattagggat cgaaacagat
    20941 gaggacaatc tgattgttcg acgggatatg tccttaactg gaaaaaatgt ttgtcgagtg
    21001 aatggtcata ttatcacatt agctaatctg agaagaatcg gcagttattt agtagatatc
    21061 caaggacaaa acgaacatca ggaactgctc cagcctgaat cacatcttgc tttattggat
    21121 cgttttggag atgtagcttt ccaacagaag aaaaagagtt atcagcaaga atatatctct
    21181 tatcgtgagc tggaaaaaag ggtacgaaaa gtgcagcaaa atgaaaaatc ctatgttcag
    21241 agaatagata tgctccattt ccagcaagaa gaaatagctg ctgctgattt gcaagtcgga
    21301 gaagaagaga aattaaaaga agaaagagaa aaacttagca attatcaaaa aattgctgat
    21361 ggattagctg caggttacgg agcactgacg aataatgaac aaaacagtgt agatggtgtt
    21421 ggcttggccg tttcagaact gcaagggatc gcccatctag atgtggagta tgaagcaatt
    21481 tatgaaaata tccaaagtgc ttactatttg ctgcaagatg caatcggcga tatgagtcgt
    21541 cagattgatt tattggagtt agatgagagt cgtttagaag aagttacgca acgtttagaa
    21601 ttaatccgac agttgaaacg taaatacggc gaatccattg agtcaatatt ggcttactat
    21661 gacgaaatca ccgaagagct agcttcttct gatttttcag aaggtcaatt ggacaaaatg
    21721 aaagaggaac tagagcaaaa agagcttcta cttcagcaac aagccgcaga tcttcatgaa
    21781 gcgcgtaaaa aaatcgcaaa agaattggaa aaatccattc tgcatgaatt gaaaagtctg
    21841 tatatggaaa acactgagtt tgaggtacgt ttcttaaaag aagaaaatag acaattgaat
    21901 catgatggat ttgaccaaat cgaattttac attacgacca atccaggaga gccactgaag
    21961 cctttggtga aagtcgcttc tggtggcgaa ctgtctcgga tgttgcttgc tttgaaaact
    22021 attttttctt ctgagcaagg tgtgacaagt attatttttg atgaagtgga tacaggtgtg
    22081 agcggaagag ttgcacaggc catcgcagat aaaatttctc aaatatcgaa atactctcaa
    22141 gttttgtgca tcactcatct gcctcaagtt gctgcagtcg ccgactatca atattatatt
    22201 gtgaaagcag tgattgatgg acgtacgcaa acttctgttt cagagttgaa aacaaaagaa
    22261 agagaagaag aaattgcacg tatgctggct ggaagtgaga ttacaaaact cacactcgaa
    22321 catgcaaaag aactattgaa actcgcaaaa aggacctgag ccacctcgca gtggttcagg
    22381 tccttttcag cagctgatag ctatatttta tttttacatg acactcatta cagcagtcaa
    22441 tacaacagat ccaatcgtta aaatcagcat tgtccaaatg acaaattttg ttactttagc
    22501 aaaagtactt gtttgttttt tttcacgcat tgttttcaac ctcgtcatta attagttgat
    22561 tcaagtgtac aatctttttt ccatttccac aagagggctt ttttataaaa acttcccttt
    22621 ttttaaataa taggtagtta cgatacaaat aaataatgtt aatagaacta gccatataaa
    22681 ggcatcatcc ctttcagcaa taggtaactt aatgttcatt ccatagatac tgccgacgat
    22741 tgtcggtata gttaaaataa tcgttaaaga agttaaaatt ttcataatat tattcagatt
    22801 attcgatacg attgctgaaa aagtctgatt catttgttct actagtttta attgaatgcg
    22861 agaagtagtc attgcctgct ctgtttccac caatacatca cgtaaatgat ttttcaaagc
    22921 atgattttct tggaaaagtt tattttgcgc tagttctttc aatgcatcta aattagcaga
    22981 agtcgcttct ctgaaataaa cgaggctttt ttggatatcc atgatttgat aaagttgttt
    23041 gttttcagtg gatacttgaa gtttggcttc caaagtatcg cattgagatt gaagcgtagc
    23101 taaataactg ttgtaagaga gtgtgacctg ccaaatgaat tgaaagaaga tattgagatt
    23161 aggcgaaata tcactttttg gaaaggtctg gctaaagact ttctttaaaa acagtggttc
    23221 atgattcgta attgtgataa gcttcttttt gggcgtcaaa ataatagaga gcgggtatgt
    23281 atccacttga aaataaccgc taggacttgt tttagcaaaa ggatattgta acaataataa
    23341 cgctggcttt gtcaactttg attgattgaa ttcttctgta cgggaatttt ctgcatcgtc
    23401 caatatggaa gtgatgtaat ctgacggaat ttcaaattga tgtgtcagct gatgaatctc
    23461 ttcttgatta ggacgttcta cacaatacca gatcgtttgg tcagtttcct ttccggtatt
    23521 acgcagtttc tcattatcta atgtaaaata tttgatcatg attttcctcc tgttgcctgt
    23581 ttcttttatt ataacggata gggaattgga atacatggaa tgtgtttgta agatatagaa
    23641 tataactgca taaatttaaa taaaatgaaa cagttgtgta aagtatatac agtatacttg
    23701 aagcaacgat aaaaaaggag tttacgttca tgggcacatt cacaccactt atcttgtttt
    23761 ggtatattta cccggtaatc gttttgtttg gttgccaatt tcttgtaaaa ttattttcgt
    23821 taaatcgtcg ttttaaaata aaagcccctg atttggcgat tccttttttg tggggcggta
    23881 tgcatgcatt gtctagaaat acagggacaa tttctatctt accgtttttc tttatttctg
    23941 ttttgctaat gggaatttgt attgctgttt tccaagcata ttattatgaa gaaattattt
    24001 atccgcgtta tttcaagatg acttggcgtt tggtttttct gttgacaatc gtcttgtacg
    24061 ctgtcttgat cattgtgaac atactttctt acctatgaaa aaaatagaaa agctgtaaaa
    24121 agtcttttaa aaggcttttt gcagcttgat ttttaaaaaa atgtttatga ataaaaaaag
    24181 ttgttcccgg atttcccaga aaaggtggta gaaagtgggg gattgtggta gactaatttt
    24241 agttagtgga ggaatggggg gctttcaatg ttgatgggtg aattccaaca taatattgat
    24301 gcaaaaggac gtttaatcgt gccctcaaaa cttcgtgaag aacttggcga aaagtttgtt
    24361 ctgacacgcg gattggatgg ctgtcttttc ggctatccga tgagcgaatg ggaaaatctg
    24421 gaagctaaac tgaacgaaat gccgttggct aaaaaagacg cacgtacttt tgtgcgattc
    24481 ttctattcgg ctgccactga atgcgaactt gataaacaag gacgaatcaa tatacccagc
    24541 acgttaagaa attatgcagc tttgacaaaa ggttgcgtag tcatcggtgt atctaaccga
    24601 attgaaattt gggatgagac acgatggcaa gatttttctg cagcagcaga agagaacttt
    24661 gacgaaattg ctgaaagtat gattgatttc ggactataag aataggaaga ggctgactaa
    24721 tgactgaaac atttcaacac tatacagtca tgttgaaaga aacggtagat ggattgaaca
    24781 tcaaaccgga tggcgtttat gtagactgta cactcggcgg tgccggacat agtgaatatt
    24841 tattaagcca actaagttct aaagggcatc tgtatgcgtt tgaccaagat caaaaagcaa
    24901 tcgatcacgc caagctgcgt ttagcttctt atgtcgaaca aggtatggtc acatttatca
    24961 aagcgaactt cagagaatta gaagaacgct tgagtgagca tgtagataag gtagacggca
    25021 ttttgtatga tctaggggtt tcctctcctc agctagatga agcagaacgt ggatttagtt
    25081 atcatcaaga tgcaccgttg gatatgcgca tggatcagtc agctccttta tccgcttatc
    25141 acgtaattaa tgaatattcc taccatgaat tagtaaagat tttcttccgc tatggcgagg
    25201 agaaattttc caaacaaatt gctcgtgaaa tcgaacgagt acgaaaaaca aaaccaatcc
    25261 aaacaactgg tgagcttgtc gaaatcatta aacaagtcat cccagctccc gcaagaagaa
    25321 aaggcgggca tcctgcaaaa agaatttttc aagctgtgcg gattgcagta aatgacgaac
    25381 tcggtgccga agaggcctct ttagaacagg caatccggtt attgaaagtc aatggaagaa
    25441 tcagcgtaat cacattccat tcattggaag acagaattgt caaaagtatg tttaaagaat
    25501 atagtacggt acaagatcta ccaccgggac ttcctgtcat acctgacgaa ttccaaccag
    25561 aactgaaagt cattactcgt aaaccaatcc ttccttcaga ggaagagcta acagaaaaca
    25621 atcgatcacg cagtgcaaag ttacgtattg cagaaaaaat aagaatgaaa gaggagtaga
    25681 atgatggcag aattaaagaa aatgcaagat tacgaatatg atttacctga attggtggat
    25741 cagccggaac aacaaatgcc tgattcttct tcgaatcaag aaatcattct tccacaatca
    25801 ccggcaaaac gattaaagca gatttctgct ttggaaaaaa ttgctgtggc aagcgtggcg
    25861 attgctattg ttgctttatg tgttttaaca gttatgctgc gcacaaatat cagcggagct
    25921 gaaaaagaaa tcacgtcaat acaaacagaa atcaatgata aaaagcaaga aaaaacgagt
    25981 ttagaacaag aaaaaaacga actttctcgt actgaacgga ttaaacaaat cgcagaagaa
    26041 aaaggattgt ctattaatga cgacaattta aggaaagtga agtaaatgag tcttaaaaat
    26101 aaaattcggc ggtttatgga aaagaaaaat ctaaatccga tgaacaatcg caaaaaagta
    26161 ggtatcattt tatttgctac gagtattgga ttgttctttt tatttgccgt ccgtttctcg
    26221 tatatcgtta ttggaggtca tgttgctggt acctcattag ctgaaaaaac aaaacagttg
    26281 taccaaggaa gtgaagtagt aaaagcaaaa agaggaacga tttatgatcg taacggtgtg
    26341 gcgctcgcag aagatgcctc ctcttattca attaaagcga tactctcaaa aacatatacc
    26401 tctggggata aaaaactgta tgttgaagaa aagaattttg ataaaatagc agaaatcctg
    26461 cataagaatc tttctataga caaaaaagat gcactgaata ttttagagga cggcgcaaaa
    26521 aaagagctgt atcaagttga atttggtagc tatgggaaaa atatctccca agaaaccaag
    26581 caaaacatcg aagcggacat gaaaaaagaa ggtgtcgctg gtctttattt tgtcgaccat
    26641 caggctcgga tgtatccaaa tggcgtcttt tcttctcatt ttattggata cgcagttcca
    26701 gacaaagatg agaatggctt agttggtaaa ctaggtttgg agtctgctta caatgatatc
    26761 ttgagcggta aagatggtaa gattatttat caaaaagata attttcaaaa tcctctgcca
    26821 ggtacagtgg cagaagaaga aaaagctgtc gacggacaag atatctacac gactttagac
    26881 agcagactac aaagttattt agaaacactg atggatcaag taaatgaaga atatcagcca
    26941 gaagaattga cagcagtcct gatgaaagca aaaaccggag aaatcttggc aatgggacaa
    27001 cgacctactt tcaatcctga aacaatggaa ggattgactg gaaaagatgc tatctggcgt
    27061 aatttcttgg tacaagatag ttatgaacca ggatcaacca tgaaagtttt cactactgca
    27121 gctgctatcg aagaaggaga attcaacgag aatgaaacct tccaatctgg aaaaatccaa
    27181 gtagaagatg ctacgatcaa tgaccatgac tttggtgaaa aaggtgtgct gaccatgcgt
    27241 caagctcttt catggtcaag taacgtcggg atggtcatac tggaacagcg attaggcgga
    27301 cgatggtata actatttaca aaaactggga tttggacaaa gtactcattc tggattagat
    27361 gatgaagtaa acggtgcttt acctacatta aatattgtcg accgagcaat gagtgcttat
    27421 ggacaagcgg taggtgtgac gaatttccaa atgatgaaag ctttcacttc tattgcgaat
    27481 aacggtacaa tgattcaacc tcgatatatc agcaaagtgg tggatcccca aactggagaa
    27541 gagcgaacaa ctcaaacaga agttctaggg caaccttttt caaaagaaac aacagaaaaa
    27601 gtccgtgaat atatgagaga tgttgtcgaa agcgaaaact acggtagtgc ttatggtgta
    27661 tatagtgtac ctggatacaa tgtatcggca aaaaacggaa cagcccaaat cgcttctgat
    27721 acagggggat atcaaacagg agatacagcc tacctgtatt cgattgtgga gatggttcct
    27781 tctgaagatc cagactatgt cttgtatcta acaatgaaac accctaagac atatgatcga
    27841 atggcattgg ctaaaatcgc aaatccattg atgaaacggg caatggattt taaagaaact
    27901 gaagaagatt cagatacaga aacaaaaaca gaaaaagtat ccgtagccga ttatcgtaat
    27961 cttgaagcgg acgttgcagc agctgatgct caaaaaagtg gactgcaacc tgtagtgatc
    28021 ggtaatggta aaaaagtaca aaaacaatct actgccaacg gtgatcagtt gatctctgga
    28081 gaaaaactga tcctatatac tggtggtgat aagctgatgc ctgacgtaac aggatggtca
    28141 aaagcagata tcatgaagct ggggaaaatc ctcggtattg aagttagttt tgatggagat
    28201 ggttattgtg tcaaacaaga acttgctcca tatgaaaaaa tcactgaaga caaacttagt
    28261 tttactttag aagaataaga agagaaatag gagagaaaag agcatggaat ggacacaagc
    28321 attgattccc attgtcagca gttgtgcaat gacaattgcc gcaatgccgc tatttattgg
    28381 ttattttcaa atgaaaaagc aaggtcaggc gattcgtgag gagggaccaa agtggcacaa
    28441 tagcaaagcc ggaactccta caatgggcgg cttagtgttt ctgatcggct ctattttgac
    28501 aggaatttgg gttggagctt ggcaaaaaca gctaactcct actcttttca tcttgctttt
    28561 tgtattagcg ttatacggag taatcggctt tcttgatgat tttataaaaa tattcaagaa
    28621 aagaaacatg ggactgaact cgaagcagaa attgattgga cagattctag ggggaatcat
    28681 cttttatctt gtttaccgtt ctgaaggtta tccaggtgta ttgaatttct tcggactaga
    28741 tctaccttta ggaatcattt atggtatttt cgctctcttc tggcttgttg gattttcaaa
    28801 tgcagtaaat ctgacagatg gcatcgatgg attagttgct ggcttaggaa gtatctcttt
    28861 tgcagcttac ggtctaattg cttggcatca gcatcagtat gatgtattaa tcatttgctt
    28921 aagtgtttta ggaggtttac tgggtttctt cgtctacaac agaaaacctg ccaagatttt
    28981 tatgggagat gtcggttcat tagcgttggg cggactttta gcggcaattt ctataatgct
    29041 aaaccaagaa tggacattgc ttttgattgg tttgatctat gttatggaaa cagcaagtgt
    29101 gatgcttcaa gttacttcct ttaagcttac aggaaaacga atttttaaaa tgtctccaat
    29161 tcatcatcac tttgaaatgt gcggatggtc ggagtggaag attgatacag ttttttggtt
    29221 gatcagcatt gtgacttcgt tgatcacatt atggatcgtt tggtaggaga tgacgggaaa
    29281 tgaaaaaaat atcaacatat gaaaataaga aagtgcttgt cttaggactc gcaaaaagcg
    29341 gagtaagtgc ggccaaatta ttacatgaat tgggcgcact tgtcaccgtt aatgatggaa
    29401 aaccttttga cgaaaatccg gaagcacaag agctgctttc tttagggata aaagtaataa
    29461 caggtagtca tccaatcgaa ttgttggatg aagaattttc attgatggtc aaaaatccag
    29521 ggattcccta cagtcatcct ttagtagcaa aagcccaaga aatgggtatt ccggtaatta
    29581 cggaagttga gctagcttac gaagtagcgg aatgcccaat tattgggatt acaggaacga
    29641 acggaaaaac aacgacaaca acgatgacag gactactact gaatgcagga gcagatcaag
    29701 gaattgcacg tttagcgggt aatattgggt atccagcaag tggtgtagcg caagaagcaa
    29761 aatcagaaga taaaattgtg atggaattgt ctagcttcca actcatggga atcacagatt
    29821 tccgccctca tatagcggtg attaccaata tctatgaagc acatattgat tatcatggta
    29881 caagaaaaga atatgtcaaa gctaaatgga accttcaaaa aaacatgaca gaaaaagatt
    29941 atctgatcct taactggaat cagtcagaac tacaagaatt agcgcaaaga acaaaagcac
    30001 gcgtactgcc tttctcgaca aaagaagtgt tggaagacgg tgtgtatgct gacgattact
    30061 caatatacta taaaaaagaa aaaataatgg aaatatctga acttggcgta ccaggtaagc
    30121 ataatgtaga aaatgcacta gctgctattt ctgtagcaaa attgtatggt atatcaaatg
    30181 aagctatacg agaaacgttg cattttttcc atggcgtacc acatcgtacg caatatgtag
    30241 gagaaatcca aggcagaaaa ttttataatg attcaaaagc aaccaatatt ttagcgacta
    30301 aaatggctct aagtggattt gaaacgtcta aggtcgtgtt attggctggc ggattagaca
    30361 gaggaaatac atttgatgaa ttaattcctt cattaaaagg aatcaaagca atggtcgtat
    30421 ttggccaaac aaaagaaaag ttgatggatg ctggaaaaaa agcaggcatc gagacaatag
    30481 taacagccga ttctgtagaa caagcagttc ctttagcatt agaaaacagt acagatggtg
    30541 atgtagtttt attatcacca gcgaatgcca gctgggatca atacccaaat tttgaaacta
    30601 gaggaaacag attcatggaa gctgtgaacc gattaaaata gaaaaagtag aagcgagcga
    30661 taaaaaatga aaattttggt tacaggcgga ggaacagggg gacatattta tccagctctt
    30721 gcctttgtca attatgtaaa gactcaagaa ccgaatgctg aattcatgta tgtgggagca
    30781 aaacgaggtc tagaaaacaa aatcgtccct gatacaggaa tgcctttcca taccttggag
    30841 attcaaggat ttaaacgaaa aatttccatg cataacatca agacggtccg attgtttctt
    30901 aagagtatcc gtcaagcaaa aaaaatcctc agtgattttc aaccagatat cgttatcggt
    30961 actggaggat atgtttccgg agcggtagtg tacgctgcag ccaaaatggg tattccaaca
    31021 gttatccatg aacaaaacag tgtaccaggt gttaccaata aatttttaag ccgatatgtg
    31081 gatcggatcg ctctttcatt taaagatgcg gcttcctttt ttcctgaaaa caaagcagtt
    31141 cttgtaggta atcctcgagc acaagaagta gcagatacta aaaaatcaga tattttgaaa
    31201 acatttgatt tggatccaga aaagaaaaca gtcttgattt ttggcggtag tcaaggagca
    31261 cttaaaatca atcaagcagt atcagttttt ttaagtgaat atattccttc ttcttaccaa
    31321 gttttgtatg cttcaggaga tagatattat gaagagatcc taaataaaat aaatcctgaa
    31381 atacaaaata ttagtgtcca gccttatatc aaaaatatgg cagaggtcat ggcttccagt
    31441 gatctgctgg tggggcgtgc tggtgcaact tcgatcgcag aattcacagc tctggggtta
    31501 ccggcagtgt tggttcccag cccatatgtt acgaatgatc atcagactaa aaatgcaatg
    31561 agtcttgttc acgcaggagc agcaaaaatg attgcagata atgaactaac aggtgaatca
    31621 ctaagtcaga cagtcaacga aatcatgggt gatgaagaat tgcaaaagca aatgtgccga
    31681 gcttctaaag aacaaggaat ccctgatgct tcaaaacgtc tttatgactt agtaaaacaa
    31741 ataatcaaaa aataaaggcg gtggtcttag tgagtaagaa aaaagaacca aaaaaattgt
    31801 ctcatccgtc ttctgatgaa aacaatttga ctccttggca aaaggctaac cgaaaatatc
    31861 tggcagaaca agagatgaaa aaccaagagc caaaaaaaga agacataaca ttagatacac
    31921 ccctttcaga ttctgaagaa gaaaaaacgg ttcaagagga acagcaagaa gaaagccagt
    31981 ccgagaaaaa aatcgaattt tttgaagaga tcaagcaaaa gaaaaacggt ggaccatata
    32041 acggttcttt tttgaaccgg ctgcccaatt taaaaagtca acggcacaaa gtactatttc
    32101 gtcggttagc tttgatcatc actgtattgg gtataccgct gatttttttg atctattatg
    32161 tttctcctta tagcaaatta caagcagttg cagtatcagg caataagaca gtcaacagcc
    32221 aagaaatcat ctctgataca aaattgtcat taggagaaaa tgtttgggga cagtattttc
    32281 atcgatctac ttatatcgat cgccttaaaa aagcacagcc aagaatcgaa acagcaaata
    32341 tacatttcaa agggatgaat gaattcgatt tggatgtaac agaatataaa gagattgctt
    32401 tgatcgcaaa aaacaatcaa tactacccag tgattgaaaa tggaacagta ttagatgaaa
    32461 aagtagcaaa tcctacaaaa aatctcccta tcttggaaga gttcaaagac tctgcaaaaa
    32521 tcaaagaatt aacaaagcaa tacaatcagt tatcatcaga attacaaaaa gcaatttctg
    32581 aaatcaagta cacgccaaga gcaagcaata aaaatctgat ccaattgaat atgaatgacg
    32641 gcaatcaagt tattgtaaat atcaataatc ttgcgaacca aatgaaatat tattcgcaag
    32701 tagccaaaga tatggatgaa aaaggtgtga tcgacatgga agttggtatc ttctcgcatc
    32761 cttacggtag cactcaagaa acggaagaat cgggccaatc ggaagaaaca actgaaagtg
    32821 aagaaaatgc ggatcaaaca aacgaatcaa gtgaagaggc agaaacgcaa aaagcacaaa
    32881 cacctggatc gtctagcaca aacggatcat ctgattcaaa tgattcaagt gaaacaagtc
    32941 aaatagaaga taatccaagt acaactccgt caagtttaaa tgaaaattga taaaacaact
    33001 ttaaaaagta agaatgtact ttctaaaaac cagtaattta tggtagaatt ggaagcagtg
    33061 aatatatcta ttaataagta tattttaaaa cggacgctag ttgttcgctt gtatatggaa
    33121 tagtaagtag gagggaatcc cattcatggc aaaaacagga atgtatgtag gccttgatat
    33181 tggaacgaca tctgttaaag ttgtcgtggc tgaatatatc gacagccaaa tgaatattat
    33241 tggagtagga aacgcaaaat cagaagggat taaccgaggc attatcgttg atatcgataa
    33301 aacggtccaa gcgatacaac gagcagtacg acaagcagaa gaaaaagcag gtatccagat
    33361 caaaggcgta agtgtcggtt tgccagcaaa catgcttgaa gtagaaaatt gccagggaat
    33421 gattgcagtt aacggagatt caaaggaaat caccgatgaa gatgtcagaa atgtggcttc
    33481 agctgccctt gttcgttcaa tacctcctga acgtcaaatc gtgtcaatat tgccacaaga
    33541 ttttactgtc gatgggttcg agggaatcaa agatcctcgt ggaatgatcg gcgtaagatt
    33601 agaaatgtac ggtctgttgt ttacaggacc taaaacgatc atccataaca tacgtaaatg
    33661 cgtggaaaat gccggattaa tcgtcaacga aatggtgatt actcctttag cgctgactga
    33721 atcaattctt tctgacggag aaaaagattt tggtacgatc gtcattgata tgggtggcgg
    33781 ccaaacaaca acggctgtta tgcatgataa gcaattgaag tttaccaatc ttgaccaaga
    33841 aggtggcgaa tttgtcacta aagatatctc tattgtatta aatacgtcat ttaacaatgc
    33901 agaagcgttg aaaataaact acggggatgc atacccagaa cggacatcac cagatgaaga
    33961 atttccagta gatgtgattg gtcagtcaga accagtgaaa gtcgatgaac gctacttgtc
    34021 agaaattatt tcagctcgaa tggaacagat tttcaacaaa gcaaaagatg cattggacca
    34081 aatcgaagca ttagagttgc caggtggcgt cgtattgaca ggcggagctg ctagtcttcc
    34141 tggtgttgtg gacttggcac aagaaatctt cggcgtaaat gtaaaattgt atgttccaaa
    34201 tcacatgggg ctgagaaacc cggtatttac aaatgtaata agtatcgtgg attattcagc
    34261 aaacttgagt gaagtgtatc aattggcaaa aattgctgtt acgggtgaaa cctcagcagc
    34321 tcgtcaaatg gtagttgaac aagaaacaac aaacacatat gagagctatg aagcacctga
    34381 agaaacaatc tacgatgagc cggaaccaaa agagtccgga gaaaatgtaa aaaataaaat
    34441 caagggtttc tttacaaata tttttgatta attcatcctg ggaggaataa gatatggaat
    34501 tctcaattga taataatatc aatgacggcg cagtaatcaa agtcatcgga gtcggtggtg
    34561 gcggtggaaa tgctgtaaac cgaatgattg aagaaaacgt taaaggtgtt gaattcatta
    34621 cagccaacac agacgtgcaa gcattaaaaa attcaaaagc tgaaacagtt atccaattag
    34681 gccccaaata tactcgtggt ttaggtgctg gttctcagcc agaagttgga caaaaagcgg
    34741 cagaagaaag tgaacaatcc ttacgcgaag ctttagacgg tgcagatatg attttcatca
    34801 ctgcaggaat gggtggagga accggtacag gtgctgcacc aatcgttgca ggtatcgcga
    34861 aagaattggg cgcattgact gttggtgtcg tgacacgtcc attcactttt gaaggaccga
    34921 aacgtggccg ttttgctgca gaaggtatcg ctcgtttgaa agaaaatgtg gatacattat
    34981 taattatctc aaataaccgt ttattagaag ttgtagataa aaagacaccg atgcttgaag
    35041 ctttccgtga agcagataat gttttacgac aaggtgtaca aggtatctct gatttgatca
    35101 cagcgccagg ttacgttaac cttgactttg ctgacgtgaa aacagtgatg gaaaaccaag
    35161 gtacagcatt gatggggatc ggtgtggcta gtggagaaga tcgcgtagta gaagcaacga
    35221 aaaaagcaat ctcctctcca ttgcttgaaa catctattga tggagctgaa caagtacttc
    35281 ttaacatcac aggcggtttg gatatgacat tgttcgaagc acaagatgct tcagatatcg
    35341 tagcaaatgc agcaactggc gatgtaaata tcattctagg tacatctatc aatgaagaaa
    35401 tgggcgatga aatccgtgtg acggtcatcg caacaggtat cgatgaatca aaaaaggaac
    35461 gcaagtctag ccgaccagct agacaagcac aaatgcaatc acctgctcaa aagactgtat
    35521 tagatatgga tcaagctaaa ccaacttctt cagaagaaga aaacagtttt ggcgattggg
    35581 atatcagacg tgaacaaaat gttcgtccta gagttgatga ttccaatttt gacaatatcg
    35641 aaaaaaaaga atttgataca ttcaaccgcg aagaaacaaa atctcatggc gatgatgaat
    35701 taaatacgcc accgtttttc cgtcgtaaaa aataaaggag aggaagagca catgattgct
    35761 gacaacttgc agaaaatcaa gcaagaaatt caggattcgt gtgctcttgt tcatcgtgat
    35821 ccgaaagaag ttacattagt agctgtaaca aaatctgttg aatccgctca agctgaagaa
    35881 ttggttagag ctggcgttat gaattgtgca gaaaaccgag tagataaatt attagaaaag
    35941 aaacaagatt tatttgattt tcctgaaata aagtggcatt taattggtaa tttacagcgc
    36001 agaaaggtaa aattgattgt aaatgaaatt gattactttc atgcgttgga cagtttaaga
    36061 ttagcagaag aaatccagaa gcggttagag aaagaattat cctgttttat tgaagtaaat
    36121 gtttctggtg aggaatcaaa acatggaatt tcaccagatg agcttttacc gtttgtggaa
    36181 tctttagctg cctgcacaaa aatcaaggtt gttgggttga tgactatggc gcctaaagaa
    36241 gcttctgatg agcaaatcca taaagtgttt ggtacgctga aagaattgcg tgatcttgtt
    36301 caagcaaaag gttatgccca tgcaccatgt agtgaattaa gtatggggat gagtcaagac
    36361 tttcaaattg cgattgaaga aggtgcaaca tttatccgtg taggaacagc gctattcaaa
    36421 gacgaataaa ggaggaacaa aaatgccgct tttaaacaga ggagcaattg ctaatttttt
    36481 cggattagct gatgaggaag agtacgaata ccaagattat cctgaaacaa caaaaaaggc
    36541 tgtacagcca actaaaaaaa atacaacaca gaatcaatca actcattctg tatctgctgc
    36601 gaatactcat gaaaaacaaa aaacggcgac ttcacgtcct gtaacgcatg aaagtattca
    36661 gccggctgaa aagaaaaaag taattgaact acatcaatca cctacaaaaa ctgtagcgaa
    36721 agaaagaaca gcttcaagaa cctctacaca acctaaagca aatcttgctg gaaaaatcac
    36781 tgtaatggaa ccaagagctt attctgaagc tatggcgatt gctaaacgaa tcattgctgg
    36841 agaagcaacc ttagtgaatt ttcatttagt agaagaaaaa caagcgagaa gaatcgttga
    36901 ttttctaaca ggaacagttt atgcactaga tggagatatc aaacgtgttg gagatgagat
    36961 ttttctttgt acaccgactg ggatggagat tgacagttca accgctcaat cttttgcaga
    37021 tgcgaatttg tttgatctgt aagaggagga actacgattt atactattgc actattactt
    37081 catttacttc aacgggcagc ctatttctat agcattatct tgatggtata tgcattgctc
    37141 tcttggtttc caggcgggta tcaatcaggg ctaggacgat tattagctaa gatctgcgaa
    37201 ccgtatatca gtttgttcga tcgtctgcct ttgaggattg gacccttgga tttcacaatt
    37261 ttaggggcaa tcctgatctt gaacttagcc gtacaaggtt tagcacagat cgttatatgg
    37321 ataactgtcc atttagttta ttgattgaag caacaaaatc tttattctca gaatagatag
    37381 gaggggaaaa ttttggatgc aaatgtatac cagcactttc gggcagatga acgtacattt
    37441 atcgattcgg taggcgattg gattgaacag gttcaaggac aatatgcacc ctatctgacc
    37501 gaatttcttg acccaagaca atcctatatt ttagaaacac tgattcgaca aagcagtgat
    37561 ttgcgtttca tgttttatgg cggatatgaa caggcagaaa gaaaaagatg tttgattttc
    37621 cccgattatt acgaacctgt agaggaagat tttgaaacag cattgtatga aatccattat
    37681 cctagcaaat ttgccagcct aagtcatggg aaaatccttg gaactttagt gggaacgggg
    37741 atcaaacgct cttactttgg cgatatcatc tcggacggtg atcgctggca agtatttatt
    37801 gcaaatgagg tggatcattt tgttgctgca caagtaacaa agattggaaa ggttgctgta
    37861 cgcttggaac caatcaaata cacagaaatg attatgccga aagatagctg gacacaagaa
    37921 agaggaacag ttagttccat gcgactagac agtgtgatct ctgagattta taatatttcc
    37981 agacagcggt ccaagcagct gatcgaagct ggtaaagtaa aagtgaactg gacagaaaat
    38041 acaagacctg attttgtact tgaactatta gatattattt cagtcagagg ttatggcaga
    38101 gtccagatcc aagaacttga ggggaagacg aaaaaagaaa agtacagggt actgttcggt
    38161 gtattacgta aataaaataa aaaggtggtt tactcatggc tttaactcca ttagatatcc
    38221 aaaataagac attcccgaca aaaatgcggg gatataatca agacgaagta gatgatttct
    38281 tagatttagt tgttcgtgac tatgaggaat tgactcaaag aaatcgagaa ttagaaaaag
    38341 cagtcaaaca ttctgaagaa aaacttgagt acttcaatga attaaaagat gctttgaacc
    38401 aatcaatcat cgtagctcaa gatacggctg ataaagtaaa aacaagtgca agcaaggaat
    38461 ccgaagtgat cgttacttct gcacaaaaca aagcagatga gttagttgcg aacgctgaaa
    38521 aacgtgcaca tcaattaaca actgatgcag aagaaaaagc aagaaaaatc ttgacagatg
    38581 caacagaaaa agcaagacag cttgcgacag aaactgaaga tcttaagaaa aaaacacgag
    38641 tattccatca acgtatcagc ttgatgcttg agtctcaatt agaacaagtg aaaagtccgg
    38701 aatgggatga aattttacaa ccattctcca gctacgtgac tgacagccat gaagtgatca
    38761 aagaagtttt agcaaaacag cttgacaatg aaaatgatac tgatgtaaac tcagacacag
    38821 gagtcataga agcacctatg acggcatttg atacacaggc tattgatatc tcgatcgtgc
    38881 ctggtgaaaa tgaaaacgag tagaacagaa aagcactttg ttattcttaa agcgagccga
    38941 tgagagtgga agttcggtaa gacactgcat ggtgaaagat cctctacttg agacctgctt
    39001 ttccgaaatt agagtaagaa gcagcggctg acctcgttat tggtcacaga gagtttgcac
    39061 tagatagatg caactaaatt tgggtggtac cacggcatgt tcgtcccttg cggctgaaca
    39121 tgcttttttg tgtccggaaa acaaataaag gagtgtatgg ttgaatgaaa atgaaagaaa
    39181 ccttgcaatt aggaaaaaca aaattcccta tgcgtggtaa tttgccaaac cgagaaggcg
    39241 agtggcaaaa agaatgggaa gaaaatcgaa tctaccaaaa aagacaagag ttgaacgaag
    39301 gaaaacctag ctttgttctt catgatggac ctccatatgc caatggtaat atccatttag
    39361 gacattcatt gaataaaatc agtaaagaca ttatcattcg ttcaaaatca atgtctggct
    39421 atcgtgcacc atatgttcca ggatgggata cacatggatt gccgattgaa caagtattgg
    39481 ctaataaagg cgtgaaacgt aaagaaatgt ccatggcaga atacttggaa aaatgtcgtg
    39541 aatatgcact ttcccaagta gataagcaac gagcagattt caaacgccta ggagttgcag
    39601 gagattggga acatccttat gttacattag atccttctta tgaagctgca caagtacgcg
    39661 tatttggtaa aatggctgaa aaaggctata tttacaaagg attgaaacca atttactggt
    39721 ctccgtcaag tgagtcttct ttagcggaag ctgaaattga atataaagat gtaaaatctc
    39781 catctatttt tgtagctttc aatgtcaaag acggcaaaga tattcttgat gaagatacag
    39841 cttttgtcat ttggacgaca acaccatgga cattgccagc aaaccaaggt attgctgtaa
    39901 atccaaaatt tacgtatgta ttggtggaag ccgatggccg taagtttgtc gtagcgaaag
    39961 atttgctaga aacagtccaa tcagaaatcg gttgggaaaa tgtaacagtt ctaaaagaat
    40021 ttgctggaca agaaatggaa tatatgactg caagtcatcc attctatgaa cgtgaatcac
    40081 ttgtgatttt aggcgatcat gttactttag acgctggtac tggattagtc catactgctc
    40141 caggacatgg ggaagatgac tatatcgctg gtagcaaata taaactatct gtagtgtcac
    40201 ctgtagactc aaaaggattg tttacagaag aagcacctgg atttgaaggt atcttttatg
    40261 acaaagcaaa tcctatgatc actgatctat taaaagaaaa aggagctttg ctgaagctag
    40321 atttctttac tcatagttat cctcacgatt ggcggacgaa aaagcctgtg atctatcggg
    40381 caactccaca atggtttgca tctatcgata aattccgtca gaatattttg gatgaagtag
    40441 aaaaagtaga gtgggttatc ccatggggta aaacacgttt gtataatatg atccgtgacc
    40501 gcggcgattg ggtaatttcc cgtcagcgtg catggggagt accacttccg attttctatg
    40561 ctgaaaatgg agaagcaatc atcacaccag aaacaatcga acatgtggca aaattatttg
    40621 ctgaacatgg atcaattatc tggtttgaaa aagaagcgaa agaacttctt cctgaaggat
    40681 ttacccaccc aggttctcct aatggcgaat ttacaaaaga aaaagatatc atggatgtct
    40741 ggtttgattc tggttcttct catgaagctg ttttacgtca gcgaccagaa ttatcattcc
    40801 cagcagatat gtatttagaa ggatcagacc aatatcgtgg ctggttcaac tcaagtatca
    40861 cgactagtgt ggctatcaat ggtgtagcac catacaaatc agttatttct caaggatttg
    40921 ctttagatgg tgaaggccgt aagatgagta aatctttagg aaacatcatt gcaccagaaa
    40981 aagtgatcaa acaatttggt gcagatatcc ttcgcttatg ggtatctagt gtagattatg
    41041 aagcagatgt tcgtgtttct atggatatct taagtcaagt ggcagaagta tatcgtaaaa
    41101 tcagaaatac gatgcgtttc ttattagcga atacagaaga tttcgatcct aaaaaagata
    41161 ctgtttcgta caatgattta cgttctgtag acaaatatat gaccgttcga ttgaaccaag
    41221 taatcaaaga aatccgagaa gaagggtacg acaaatataa cttcatgcat atttaccgta
    41281 cagttatgaa cttcttgacg gttgatctat cgtcattcta cctggatttt gctaaagatg
    41341 tagtttatat tgaagcagaa gatgattatc aacgtcgttg catgcaaact gttttctatc
    41401 aaacagctgt agctttgaca aaattattga ctcctatcat tccgcataca gcagaagaaa
    41461 tctggtcatt cctgaaagaa gaagaggagt acgttcagtt aagcgagttt cctggttatg
    41521 aagagtttgc aaaccaagaa gaattgatgg atacctggac tgctttcatg gacttccgtg
    41581 ataatgtctt aaaagcattg gaagaagcaa gaaacagcaa attgattggt aaatctttgg
    41641 aagcaaaact aactgtttat ccaaaacaac aagttcgtga aatgttaaaa gctttggatg
    41701 cagatattgc acaattattg atcgtttcac cagactactt tgaagttatg gcagcagatg
    41761 aacaagtgcc agataacgct atggtatttg atgatgttgc tattacagtg gaaaaagcag
    41821 acggggaaac ttgcgatcgc tgtcgccaag ttcgtaaaga tgtaggagta gatgaaaaac
    41881 ttcctcatct atgcggtcgt tgtgcaaaaa tcgttgaaga cttctatcct gaagctgtag
    41941 ctgaaggatt cgaagaaaag taaaagggaa aagggggtgt gacatgttct ttgtcacacc
    42001 cccttttgtt atcaatcgtt taattttcca cgttcacgat accagttatc tggttgccag
    42061 atccattcga atccgtcttt ttcaagtagt tcgaaagctg ctttaggtcc cattgatcca
    42121 gctttgtagt tgggaaaatc cggttgtgtt tggtcccaag cttttcgaat gacatccacg
    42181 atatgccaag attgagcgac ttcttcccaa tgagagaagt tcgttccatc accattcaac
    42241 gcatctaata gtaatttttc gtaagcttct gggctatttt caaccatttc tgcactattt
    42301 ctaaattcca atttgattgg ttccgtttca aatccttgcc ctacttcttt cccattcaag
    42361 gataaagaaa atccttcagt tggctgaata tagatcgtta gaacattagg tggaagtgtc
    42421 gtatcgtcac atggttcatc gacagatgtt ttgaatacat taacaggaac ttgtttgaat
    42481 acgatgttga tccgagtccc tttttcagtc aaacgtttgc ctgtacgtac atagaaagga
    42541 acacctgacc aacggaagtt atcgatagtg aatttgcctg cgacgaatgt ttcagtctct
    42601 gattgagcat ccactttgtc ttcttcgcga tagcccttga attgtacacc atccaattca
    42661 ccttgagcat attgcccgcg aacgaagttt tctaacactt cttcttcgtt ataaatgcgg
    42721 atagctttca atgctttgat cttttctgta cggatttctt tttcggaaaa tgctactggc
    42781 ggttccattg caagtaatgc tacaacctgt aaaatatgat tttgcaccat gtctttaagt
    42841 gcgccactat gatcgtagta gcccccgcga tcttctacac cgaggttttc gccaaaagta
    42901 atttggatat tgtcgatata gcgattgttc cattgagcct caaaaatatt attggcaaaa
    42961 cggatagctg agatgttttg gatcatttct tttcctaaat aatgatcaat cctgaaaata
    43021 tcttgttctg ggaatacttc tctgatttgc tggttcaatt caaaagcaga ctggtaatca
    43081 gaaccgaacg gtttttctat gatcaaacga ttgaagccat tttttgtcat aatattttgt
    43141 gatttcaaat gagaaacgat cgttccaaaa aattgaggtg ccatagccaa ataatagaca
    43201 cggttaccgt ttaattgata gtgtccatct aatcggtcag acaattcttt taatgtctga
    43261 taatgcgttg aatcatttac atcatgggat tgatagtaaa aatggcttgc aaaggtagtt
    43321 gcttcttcat ctgaaggatt caatgcacgg attgtttctt tgacaacttc acggtagtgc
    43381 tcatcgctcc agggacggcg agcggttccg attacagcaa aatgctcgcc taaatttcct
    43441 tttctgtaaa ggcggaacaa agacgggtac aatttacgct gggctaggtc acctgtaccg
    43501 ccgaatatcg taaacaatac atttttttca ttagacatgt ggatacactc ctttatattt
    43561 tatcgacaaa aatcgtgctg gccgccttaa agctgacggg gattttcttt ctatcatttt
    43621 ggatagtgat cggtccttca taagctgcga tgtcaacgat ctcatattct tcgttgatat
    43681 tcaggtcaat agtgactaag taatccaaca attctttttc atcaagcact cgtgcgatac
    43741 gcacttttgt accgacagga taggactcca atgtttgtcg gcgttcttca tggacaactt
    43801 cctctttttt aggaatcgat cctccatgag gacaatgttc aggatgattc aaatagtttt
    43861 ctaaatgatc tgcaagatgt tccgaagtca catgttccaa tacttcagca tcatcatgga
    43921 cttcattcca gctatagtct aaatgttcaa ctaaaaaaac ttcccataag cgatgtttgc
    43981 gaatcagact gctagctttt tgcagtccgc ttgcagtcaa ttgtacacct tgataagggg
    44041 aatgttctac aagctcttct ttcacaagtt tagaaatcat ttcactgaca gatgcagctg
    44101 agacatctag actagatacg atttgtttat tgttgacttt cgttgtatct ccacctagtt
    44161 ctaaaatgat tttcaaataa tcttctcgat ttggtgtcat attcgatcat cctttcatgg
    44221 taaaaaacca tcattagtaa gtgtaacaaa aaaaacgtta attgactatt tttctaaata
    44281 aaaaacaaag gatttttaga cgtttttaca acaaaaaacg aaagatgata gagaagactc
    44341 attctttcgt ttataaagga tatatatata cattgggagg gatctatttt tcagataaca
    44401 acttatatga agtgtctgca gattcgtcat aaagagtttg ttcgggataa gaaaagataa
    44461 gataaaaacc atactgatca cgtacgacta aacgatattc atctatttct accacagatg
    44521 tgccaagaga ctgaccgttg atcaaaaacg tgtaatcaga tgtaatctgt aattgttgtt
    44581 tttgctggct atcactaaag aaccatgtac cttgatactt tgagagtcct tctgactttt
    44641 ccgcttgttt tttctttttc aagtaaataa aacttgagag aacggtaatg agtgaaccaa
    44701 gaaaatagat catttttttt atcaccaaat cactccttta tgagaaaata atcgcataga
    44761 catattcttt cttctttatt tcattgtacc gaataaaagc taaaatttct ctaagacttt
    44821 cgactggttt ttttactgtt tttctacaaa ttatgatagg atacatagta ggaagaaagg
    44881 tgacataatt tgaagtatca gcaagtggaa atagcttttt ttattgaccg cccgaatcgg
    44941 tttatagcac attgtatgaa aaaagacgga gaaataatta ttgcgcatgt caaaaataca
    45001 ggtagaggaa aagaggtcct gataccaggc gctgaagtgg ctattgtatt tgctcctgga
    45061 cctaaacgga aaaccgccta tgatctgatt gctgtaaaaa agaagggtga ctggtttaat
    45121 atcgatagtc aattgcctaa tcggctggca attgatggta ttttagacgg cacgatcctt
    45181 ttaccaggat taactggtcc atttacggta tacaagcgtg aagtaactta tcttcactcc
    45241 aaatttgata tttatttaga gacggtggtc ggacaaaaag catttgttga agtcaaaggt
    45301 atgacacttg aaaataaagc gattggtgct tttcctgatg cgcccacact cagaggattg
    45361 aaacatatag gagaattaac gtatgccgca caagatggtt atgccgctta tgtcttgttt
    45421 attgctcagt tcgaacatct tcatctggca acgatccatg aagaaatgca accagcacta
    45481 gctgatatgg ttcgacatgc gcagcaaagc ggagttcaaa tcctcgccta taattgtcaa
    45541 gtatcggcag atgaggtaac tgtcaagcag gccattccat ttgatgtaga tatccctttt
    45601 gaagatccta tacaatagaa agaaggtaaa ataatgatca acttaggtat tatcggaaca
    45661 aattggatta ctcatcaatt tgtccaagca gctttaagca caaaaaaata tgaattgaca
    45721 gcggtatatt ctagacattt ggaaaaagca caggaatttg gttctcatta tgaaggggat
    45781 atcgaattcg caacagattt gaagactttt ttcaatatta ctcatatgga caccatttat
    45841 attgcttctc cgaatagtct tcactttagt caagctaagc aagcaatttt ggcaggaaaa
    45901 aatgtgatag ttgaaaagcc agctttttcg acgccaaaag aaatggatga aatcatccat
    45961 ttggcaaatg aacacaatgt gtattttttt gaagcagcaa gaaatatcca tgaatcaggt
    46021 tttaaaaaga ttgccgatta cttaccatta aaagacgaga taatcggtgc aaatttcagc
    46081 tatatgaaat attcttcgcg ctatgaccaa gtattagatg gagaagaacc gaatatcttc
    46141 tcgactcatt tctcaggtgg tgcattgatg gatttaggtg tttaccttgt atatgcagct
    46201 gttgcttggt ttggaatgcc aaaagaagtc cattatttcc caagaaagat ttctactggt
    46261 gtagatggtc ttggatgggg gatcttacgt tatgagaatt ttgatgtatc gatccagcca
    46321 ggtaaaatcg gtgattctta tttaccgtca gagatttatt ttgactcagg aacgctagtg
    46381 atgaatggag tcaatgcaat tgaacaagca caatttcatg atcgcgcgca taaagaaatc
    46441 attgatttgg cggtaacagc tgcagaaaat ccaatggcag atgaagcaga ggattttgcc
    46501 aatgtcatgg ctcatccgaa agatccagct tggggattgt tgtatgaaga gtggatcgag
    46561 ctagctagaa atgtgaacca agtgatttat gatttaagaa aaaatggcgg catcaaattc
    46621 gatgcagata atgaggaaga tttttaatga acgaatttgt acaagtattt cccgaaaatt
    46681 ggcagaaaaa atatgaggat aaagggttta tctcaccttc tcttattcaa cgagcagttt
    46741 ttcagccact aagtaataaa caaagtatag tagcggtttc tcctacaggt tcaggtaaaa
    46801 cactggctta tctttggcct ctactactaa acgtggaacc aggtgaagca agtgcgttgg
    46861 tcatttttgc ttcttctcaa gaactagcta ttcaagtagc agatgttgca cgtgaatggg
    46921 gaaaagacaa agagttgaaa gtccaatctt tggtgggcgg agccaacgtc aaacgtcaga
    46981 ttgagggttt gaaggaaaaa ccagaaatct tgattggtac accaggaaga atattggaat
    47041 tgatgaaagc aaaaaaaata aaagcacatc aagtgaaaac aatggtattt gatgaagcag
    47101 accagctatt tgacgctggg aacagccaaa tcattgatca aattcttcat caagctccaa
    47161 cagagtatca gctagctttt ttttcagcga cagcagatcg ttcccttgaa agcattgaaa
    47221 aaatcactgg gaaaacgttt gagacaattg acgtgacagc agaggacgat tctcgaaaag
    47281 gactgagaca ttacttttta cgtgtaccga tacgaaagaa agatgactac ttgagacgtc
    47341 tcacgcatat agaagatttt catggtctga ttttctttaa tcaactgact gaattaggcg
    47401 taatggaaga aaaattgctg tatagaggtg taccggtagg aagtctggcc tctgatcaga
    47461 acaagctact ccgaaaagca gcgctagaac aattcaaaaa gaaaatggtc agagcacttt
    47521 tgaccacaga tgtggcggct agaggtttag atattacagg tcttccttat gtagtgaatg
    47581 cagaagttcc gttaagtgaa gaagcttatt tgcatcgttc gggaagaact ggtcgaatgg
    47641 gtaatgaagg aatagtaatt acactcgtac aagataataa tctaagagat ttaaaacggc
    47701 ttttacgtcc atcaaatata acacttgaag agattttttt atacggaggt aagctgcaaa
    47761 ctgagcagcc aaccaaagaa gatgcagctc ctgcagtaaa taaaaagtat gcgaaaactt
    47821 tttctggaaa ggagcagaac ccagaatata acaaagaaaa aataagaaaa aaagttaaaa
    47881 ataaatcaaa aaaagagaga aataaaggag caaggcgcaa ataagcgcct ttttatgcta
    47941 tgattatgaa aacgggaaca aaattggttg ccagaggagg ggttatgtga aagaacgtca
    48001 agaagagatc ttgcagctat tgacagaaaa ccaacaagga ttgacagcaa gtgatgtagc
    48061 tgagcgctta actatcgatc gaagcaatgc aagccgatat ttaagtgaac tatacaaggc
    48121 ccatcatatt gtaaaaaccg ctggccgacc agtcgtttac tctttgccaa cggaaaagtc
    48181 aaaatctgat gaagtgcatg tcgactcttc tacgcaagtg acttttgaaa cactcgttgg
    48241 agaaaatgat tctttaaaag tcagcataca gcaggcaaaa gctgcgattc tttatccacc
    48301 gcgggggttg catacgatta tttttggaga aactggtaca gggaagtcga tgtttgctga
    48361 atgtatgtat cattttgcaa tcgattcaga gatgctttct gcagatgcgc catttgtttc
    48421 tttcaattgt gcggattatg ctcaaaatcc gcaactgctc tttggacaca tctttgggat
    48481 caaaaaaggg gcttacacag gagcagctca agatagtccc ggtctaatag ccaaagcaga
    48541 cggcggtata ttgtttttag atgagatcca ccgtttaccg ccggaagggc aggaaatgct
    48601 gtttactttc attgataaag gtatttaccg tccgcttgga gaaagtgcac aagtacatga
    48661 agcatctgtt cagatcattg gggcgacgac tgaatcttca gaaagtttct tatctacgtt
    48721 caatcgacgg attcctatgg caatcacctt gccgaatctt gcttcacgtt cactggatga
    48781 gcgttatgaa atcatctctc tttttatcaa acaagaagcg aatcgtttga atcaacgtat
    48841 cgatatcgaa aaagaagcga ttcttgcctt catgctttat gatgcggaag gaaatatcgg
    48901 tcaagtgaaa cgagacttga aactagtctg tgcaaaatct tttttacatt accgtactca
    48961 tcatgagaaa aagctaatca ttcgaaaaga agaactttcc cttcaagtac aaaaaggatt
    49021 actgaaaatc aaggaaatac cggaacgtct cgaccgattc atggacagca aaagtcagta
    49081 tttgacattt gaaccaggat ttgctgatgt tgtctggtca caagatccag aacgaaacat
    49141 gcaggtctat aacgatattg aggaaaaagt cacttctctt tccgaaaccg gtgtggagaa
    49201 cattgatcta gagaccctca tttcaaaaga tgtggatgct tatttccaga catatgtcaa
    49261 agaattgact aaaagttcga ttcccaaaga tcttttgcca gatgaaatct ggcggttaac
    49321 caatcaattg tatgatgtgg ctgaaaaacg gttagaccgt atttataatg aaaaagctcg
    49381 ttttgctttt gctttgcatc tgcaaagtac attggatcgt gtgagagaag gtcatatgat
    49441 cgtccaccca gatttgaata acgtacggaa aaattttaag agggaattcc aggtagcaat
    49501 cgatctttca acgatcattg aagaagaaga acaagttgag attccttttg atgaaatcgg
    49561 ctttattagt atgtttcttt ctatcaatct aggagaaagt gagcctgtta aagaaaacaa
    49621 agtagatgtt ctcatattga tgcacggcag acagactgct tcaagcatgt tggagatggc
    49681 acaagaacta ttaggaacaa aagtagggaa agcttttaat atgccactgg ttatggaagt
    49741 tcaaacaatg tatgaagaag tgttacaata tgtcaaaaac tatcaagagc atttgacgaa
    49801 tggcctactc ttgttaacag atatgggatc attaaacacc ttcagtgatt tgatttatga
    49861 agagactggc attcggacga aagctatcac gatgacaagt accatgattg tgatagaagc
    49921 gatacggatg gcaagtgttg gaagaagctt agaagatatt tatcaaaata tccagctttc
    49981 ctttgaatcg atcgtacgtg atcagttcaa agcggaaaag tctgaaactt tacagcgtaa
    50041 aaaagccgta atcgtgacat gctttactgg tgaaggagta gctgccaaat tatatcagcg
    50101 tattatgcca gtgattaatc aaaataaggt agaactgatc cagatgcagt ttttagaacg
    50161 tgaatcattc aaaaaacaca tcgatgcctt attggaagaa tatgagatca aagcaattgc
    50221 gggaacagtt gatgttgatt atcaaaatat cccatttttt tcagcctatg acgtttttga
    50281 tgatgaaaaa ctaaatattt taaagcgtat agctgatgat gagatcccga cagctaaaat
    50341 tgtcgattca cttgctggat cattaagcga tgtcttttct attgatgaac tgatccgttc
    50401 attgcaaaag acagttcagc agatccagac cgatttgtcg attatcgtgg aacctggagt
    50461 agatactgga atcgttatcc atttagcctt catgttagac ggattgatca aacaagaaag
    50521 ccatcggcaa tttgagaaac tgtctatttt ctcaaaaatt tatcgtttgg aaatggatat
    50581 cgttcgggca aacttgatgg cactagaacg aaaatatcag gtaaaaattc cagaagatga
    50641 tatcgcattt cttacacaaa tgtttcttga aaataaaata aaaaaatgaa cacactaatc
    50701 atactgttta cacactaaac atctgtgtgc gcactataaa aaagagctaa aaagcatgaa
    50761 aacgctattt cagctctttt ttttattggc atgcttcttg cgttatataa aagtgtaaaa
    50821 aaataaaagg agtgttaaaa atggcaaaaa aaacaatcat gttagtttgt tcagcaggga
    50881 tgagtacgag tttattggtc acaaaaatgc aaaaagcagc agaagaaaaa gggatggaag
    50941 ccgatatttt tgcggtctct gcctcagatg cagataataa tttagaatct aagaatgtcg
    51001 atgtattatt attaggtcct caagtacgtt tcatgaaagc tcaatttgaa caaaaacttg
    51061 cgcctaaagg tattccatta gatgtcatca atatgcaaga ctatggcatg atgaacggag
    51121 aaaaagttct tgcacaagca gaaaatctaa tgaaataaat caagtatgca gttgggggta
    51181 taacaacgtg gaagatcaac aaaatctaga agccatcatg gggttaatca tgtttggcgg
    51241 taatgcaaag agcgatgcaa tggaagcgat tgcggctgct aaaatcggtg attttgagtt
    51301 agcggatagg aaaatcgctg atgcagaaga atcacttata caagcgcatc atgcacaaac
    51361 agacatgttg acacaagaag cacaaggaaa tcatatgcaa gtgacattat tgactgttca
    51421 cagtcaagat cacttaatga catctattgc gtttactgat ttagcaaaag aaatcatcga
    51481 actttatcgt cgcgtataca gcgaataaat acaacttctt ttttgaacgg aagaagaaaa
    51541 atttttgaac ggatttttta ttttgttata aggagaactc tctttatata aaaaacttat
    51601 aggaggaagt aaaaaatgaa cggactatca agttggatgg agaaatacat cctgccggtt
    51661 gccgcaaaaa tcggagcgca aaaacattta gttgctttgc gtgatgcttt tatcggcaca
    51721 ctgcccgcaa caatggcggg atcagtcgct gtaatgatca atgcaattat tcgtgattta
    51781 ccgcaacagt ttaacagtac ctatgctgac agtttagcag cagacaaagg aatctttgcc
    51841 gtattgaatg tcatcatcgg aatcaatggt tttgtctgga acggtacact agcaatcgcc
    51901 ggattgatct ttgccttttc ttgggggtat aatcttgccc gagcttatgg agtaaatgat
    51961 ctagctgggg ggatcgttgg tttagctacc ttgattcaag gtatttcctt tggtccagca
    52021 ttatcagcag cattagatgt gaatgttcct tcaaatgtcg cagatgcaat caatagttct
    52081 gagattggtg caacgattgc agatggcaca ctgtcagttg gattatgggg ttggctaaaa
    52141 ctagatcatt tgaatggaaa cgcttatttt acagttatga tcatgggagc tatttcagtc
    52201 attatttttt gtaaattgat gcttgccaac attacgatca aaatgcctga ttcagtacca
    52261 ccagcagtat ctaaagcatt tgcagccatt ttaccagcga cgatcgcttt atacgtcatt
    52321 gctattatca attatatcgt aggtcgtgta gctgatgggc aactgatcat tgatttggtg
    52381 caaaaatata ttgcagagcc attcctagga ttatctcaag ggttgggagc agtattgatc
    52441 gtcacagtat ttgttcaagt attctggttc tttggtattc atggaccaaa tgtattggca
    52501 ccagtgctag agggaatctg gggacaagca caactaatca atattgatat tttccaaaaa
    52561 ggttataacg gaaaaactgg tacaccagcc gtgttagatg caatcgaaga tggaaaagct
    52621 tatatgtggg tccgcggatc gttcgatgct tttgcatggt ttggcggatc aggcggaacg
    52681 atcgtcttac tgatagctat cttgattttc tctaaacgtg cagattatct aacagtagct
    52741 aaattatcca ttgggcctgg tatcttcaat atcaacgaac cagtgatgtt tggtctgcca
    52801 atcgtattga atgcaatcat gtttatccca ttcttgattg ctccagtagt agcaacagca
    52861 attggatgga cagctactta tcttggccta gtcgctccgg tatcgcagca agtcacatgg
    52921 gtagtaccgc cgttcttatt gtcattctta gcaacaggag cggattggcg ggcaccgatc
    52981 gtaacattag tatgtatggt cgtgactttc ttgatttggg caccatttgt catctctgca
    53041 aacaaaatgg accctgccgc aggcgaacaa caataagatg aacgtgagga gcgggacgct
    53101 ttaagtttcg ttcctcataa tgaattttcg aggaggaact gaaatgatta caattgccag
    53161 tgcaatgcaa caagatgaaa tcaaagaatt gttagaagga tacaatgaag aaggaatgac
    53221 ttttacattt aaagaaaaac aaggaatcaa gctgttcttt gaaacaagtg cagaagatcc
    53281 ggaagttgcg gctaaaaaag caaaggaact aatcaaagca cagccttggg gaacagtcct
    53341 ttattttcaa tcaacagctg tttagaaaat ggtttaaaaa gataggatgg ttgagatgat
    53401 aaaaaacggt ttatttttat gtagtattgg ttctttgatc gtgttgtata gtttgaatcc
    53461 acattcgcaa aggtatgatt tgtatagtat ggtcacaggt ctttttctaa caggattagg
    53521 aatctatttc ttcatgaagg ggaaaaaaag agaagaagca aaggagaaaa ataactaatg
    53581 gaaatcacga aaaaattaaa tattccagca tcgttttttt acgataaagt tatggactct
    53641 gtccttttcg atgtacgtaa agcgaccggc aaatcagtaa ctagaaaaca gctgaaaaat
    53701 ttggagtacg tcaaacaatt ctcgcaaaat aaccgggctc gcataaaaat cgaagaagtc
    53761 attgaaaacc agtcttataa gtttcgtaca tcgacaacga agaatgaatt cgtcgttcat
    53821 tatcaaatca aaccattgga cgattcgacc tgtgaggttc attacacaga aaaaatggag
    53881 tcttatggca tgcttcaaaa gttgaatgat atgcttttag gaacaatctt gatgtatttt
    53941 aaacggagac gtttcaaaaa aatgctagat atgatcgaag caacgtatta aatcaatgaa
    54001 acatggtact atttagttat tgaaaaatga agtaagctag aaggaatttt tcataggaag
    54061 atagatcttt cctgcgaatc ctcactagct tttttgtagg aaggggcagc aaaatgacaa
    54121 acaaagtaag agagctaggt gtatcttcga ttgaggaaat gtttgattta gccacttatg
    54181 catttaatgg ggcagataca cctgaaaggc gagaacgttt tcgtacactt gcggagaatt
    54241 cttggaatta cggatttttt gatgaggagg atcggctaac aagtcaagtc atggcgaccc
    54301 cttttccagt gaatttttac ggacaagagt acctaatggc cggcattggc tatgttgctt
    54361 cttatccaga agcaagggga caaggcggga tcaatcagat catggaaaag atccttgaag
    54421 attgccgtga tcgtaaagta agcttatctt atctggcgcc attctcctat cccttctatc
    54481 ggcgctatgg ctatgaacaa tcatttgata aaatcagtta tcaactaaac agtcgtgatg
    54541 tgccgtttat caaaaaaaaa tctggaaaaa taaagcgtat ggattttgaa gatgcaaaag
    54601 ctgcaatcag aagaatttat gaacagatgc cggaaaacca acgagctggt ctgaagcgcg
    54661 aagactggtg gtatacgtat aaattcaagc aaaaaggcaa ttaccagttt gctttgcatt
    54721 ttgatgataa tgaggaagca acgggatata tcgtttatca gcttgcaacg tcttccttta
    54781 ttattgcgga gtggggattt ttggatcatg atgctttttg cagccttgtt cgcttcgtaa
    54841 gttctcacaa tggtgctttt gaaacatttg aatattcatg cggctacggc ggtaatgatc
    54901 aaaattatct attagaaaat ccatttgctt ctgtaatgat cacaccttat atgatggcga
    54961 ggatcgtaga tatttctttg tttttggaaa attatccatt taaaaagaga caagctgcat
    55021 ttgccctaca aatagaagaa gatcattatg cgaaatggaa tgaaggaatc gtggaagtaa
    55081 caatcgaaaa tggaaaaaat catctctcaa aagtagaaca aacaaaactc ccaatcgttc
    55141 gaggaagtat ccaacatatc acgcagctat tattagggta tcgtaaagtg gaagagttgg
    55201 tattccaaga aagaatacag gtgacaaaag aatatgaaga aacgttaagc tcaatcttgc
    55261 cacagcaaca gccaattctt aatgactatt tttgataaaa aacgggtgat gacaatattt
    55321 ttgtcaccac ccttttgttt ttcatttggc atttgaagga aaatgttaaa gtaaatcctc
    55381 ttttttcgta aatttgaatt ttttgttttc ttttgtttta gggttttctt ttttgtggtc
    55441 ttggattgct tctttcgtaa tcttttcccg atttttttta gatgaatcta agcgttctgc
    55501 gtatactttt tcttttggct tatcattcat tggaaaggac ctccctacat tatttcattc
    55561 tacctctagt ttatcatctg ttaaggacta gtggaaacgt tttgcctaac ttataagctg
    55621 atttttttag cagataagct tgcaagtaaa aaacaaacaa gctacaattg atttattgaa
    55681 attgttcctg tagctcagta ggatagagca atcgcctcct aagcgatagg tcgctggttc
    55741 aattccagtc aggaacgtac aaataacccc tgtatccttt gcggtacagg gggttatttt
    55801 gtataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga tacgctaagt
    55861 atatttactc cttgataaag taaaatagat gcatgcgtat gtcttagtcc atcaataaac
    55921 agtcgaagag aaaataggga aaaattgatt actaaagaaa aacttactag gtcgacggtg
    55981 tagttgattt tcatacttca atagattcga attaaacgat ttcgattggc ttataccagt
    56041 cctatccaac gaaagatata tcgttcttaa ctctagcggt ataacttgaa gctttacgta
    56101 aagccaatgg gaatgaactg gagatgactt tttattattt ttagtaatag ctgatgggtt
    56161 aatacttaca actttccttt tcaaagtacc atataaaaaa acggtggggt tgtttagcga
    56221 aataaaggat ctaagccaat agcttagatc ctttattcta tgctttatat tattttttgc
    56281 atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta ctgtagagcc
    56341 atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat ctacgtgggt
    56401 ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg taccgccttt
    56461 cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt catcaaataa
    56521 tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta caagtgcgac
    56581 ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga catcttctcc
    56641 ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca tacccgagat
    56701 gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta ctcgatgact
    56761 gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt ctccacgagc
    56821 attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa tgatatctgt
    56881 accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt ctgctttttt
    56941 tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat agacacgagt
    57001 cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca gttctgggaa
    57061 gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga ctttatttgc
    57121 ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat aagacgctac
    57181 tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc tgatcctgct
    57241 tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta cattctcaat
    57301 acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag tccattgaac
    57361 gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag caaggggtgc
    57421 ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta cgccagtttc
    57481 tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca taataaaaat
    57541 tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta tgattcaaac
    57601 aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc atgatatatg
    57661 ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca tatcaaaaaa
    57721 acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa ggagattaaa
    57781 aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg cttatgaagt
    57841 agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca aaatgaattg
    57901 ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac aagctctttt
    57961 tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata cagggaatac
    58021 gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga agttgtatcg
    58081 ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct cttatactta
    58141 ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag agcagatcga
    58201 atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga tgtcttcaag
    58261 catcatccat ccaactgatg aacagattga aaagatgatc caagacagta tagatgctat
    58321 cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc cacaaatcag
    58381 taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag cgtatgcatt
    58441 tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata cagattgaat
    58501 aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt tctttagcga
    58561 aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta acattttcgg
    58621 ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc gatttgcaga
    58681 attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc aatagatcca
    58741 attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca tcatcgtatg
    58801 tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct acttgggtac
    58861 attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca atatcggcga
    58921 tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc atgatgtgcc
    58981 tcctcgttta taattattta acaattatga attataccaa aacatcattg aaaagaaaat
    59041 aaaacctaaa gacacctggg gaatgtcatt tggctaaatg tatgccgcta tgttaaaatg
    59101 agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca cgtgaaattg
    59161 aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg cgtgatttta
    59221 taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat atcgagagtc
    59281 atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact tgtatcagta
    59341 taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac ggtgatttgg
    59401 taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt tgggcctatg
    59461 tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag aaagctttgt
    59521 acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt catgctatcc
    59581 aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt catggcgttg
    59641 gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga aaagggttgc
    59701 ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga acttggaaaa
    59761 tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt tcttgccaat
    59821 atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca caaggagaag
    59881 aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa agtttggaac
    59941 aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa gcgcatcact
    60001 gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt gtctttattt
    60061 ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca agagactgta
    60121 ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg tcctgcaatc
    60181 aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt agcggcttta
    60241 tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc gtatggcgta
    60301 gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat tgtcttgttc
    60361 atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat tttggatgct
    60421 attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac tttgaagtgg
    60481 ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat tgtccctaat
    60541 gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt cggctggatg
    60601 ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat cagcggttat
    60661 cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc tacgatcatc
    60721 attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca tcaaatcaaa
    60781 aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt ttctcataaa
    60841 aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttata ataattatga
    60901 ctcttaatta aagtaggtgc ccatatgctt gtttctttta gtatggttat aagatttttt
    60961 ctgacaattt tattatcact gatattaaca cctgtattca aaatcatttc agtacaaaca
    61021 ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag ttccaatgcc ttcagcgggc
    61081 ggactgccta tctttatttc atttgttata tcgactttat tcttattccg taaaattatc
    61141 cctcaatcgt atattgtacc gatcttgatt gcttctttag taatcatagt cacaggagtg
    61201 ctcgatgata aatatgagct gacacctaaa caaaaaagta tagggatctt gattgcttct
    61261 cttattattt attttgtagc agatatacgt attgattcct tcacgcttcc ttttattgga
    61321 tatatccaac taggctggct tagttttccc gtaacgattt tttggatctt cggtatcacg
    61381 aatgcggtca atctgattga cggtttggat gggctggcgg cagggatttc attgatcggt
    61441 ttaattacga taggaatcat tggatatttt ttcctccatg cttctacagt gtatattcca
    61501 gttgttattt tctgtcttgt agcaagtatt atcggttttt ttccttacaa tttttatccg
    61561 gcaaagattt atttaggcga tacaggagct ctattcctag ggtttatgat ggccgtcctt
    61621 tcactacaag ggctgaagaa cgtgactttt gtttcatcga tttctttgct gatcgtcatg
    61681 ggagtgccgg taacagacac gttttttgct atcattagga gaaaagcaaa ccgtgtttct
    61741 ttttctacag cagataaaaa acatcttcat caccgactaa tcagtttagg ctttactcat
    61801 aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt tttctttcac agctatggtc
    61861 atgaattata ctggaagaat cggaacgatc gtattgatta ttgccatgtt gtttgctgca
    61921 attttattat ttgaattgat tggtctgatc aatgagaaac atcagtttat tttaaaaaca
    61981 ttgcgatttt tagggaataa agagtaccgt acgcaagtaa aacagaaata tttgaagaaa
    62041 tggaaccgtc atatagatga ataacaaatt aaaccagccg atccttatta gtattgtgac
    62101 ggcaaacagt gataaaattt ttcggacact agataatttt atttcaactg taaaagatga
    62161 aacagcagta aaaatccgaa tttttgacaa caattcactt cccgagtaca aaaaaagact
    62221 aacagagtat aggaaatatc cctacatcga tatctatttt catgatgaaa attccggatt
    62281 tggttatgga cataatcaca atctgatagc tagcgattct cgctatggca ttatttgtaa
    62341 cccagatatt ttagtggatg aagagacagt tacagcattg gtcttgttat tgaaagaaca
    62401 tccggaatgt gcgatgttag cacctaagat tcttaatgaa gatggaacaa ctcagcacct
    62461 catccgtgag cagctggctg tttttgatta cgttttgcgc tttgtgcctt tcaaatgggt
    62521 caaaaaaaag tttgacaaac gactggctgt gtttgaatgc cgaggattgc ctactgatgc
    62581 taatagttat gtacggatga tctcaggtag ttttatggta gctgatattg ataaatttaa
    62641 agagataaag gggtttgatg agcgtttctt catgtacttt gaagacaacg acttgtgttt
    62701 gagttttgaa aaagcaggtt acaagttatt gtacacaccc ttttatgcag ttatccattt
    62761 ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc gtgtttttga cttctatgat
    62821 gaaatttttc aataagtggg ggtggcgttt tttctgatga aaccagaaat attagtagtc
    62881 gttgtgcttt atcagcaact attcagtcaa tctccagctt atggaccatt aagcaaagca
    62941 cttagcgaaa agcaaattca gcttattatt tacgataata gtcctcaaag gcagcaaaat
    63001 gagctgtttg aaaaagaaaa tatcgtttat catcatgatc cgcaaaatcc tggtttagcg
    63061 gtggcttata attttgcgtt gtctcaagca gatgaaggaa cacgttgtct cgtcactctg
    63121 gatcaggata cgaggctagt cgacgattat tttgaactat tgcgtaaagt aacctttaca
    63181 gatgaatgtg ttgctgcagt tccgatgatt ttttctggga gccgacaagt ttctccccta
    63241 tattcagatc gttatatcaa tcgtacagct caagcagtag aagttgaagg cccaacagct
    63301 gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg tcaaatttct aaaaaagatt
    63361 ggcgggttta atcctgcttt ttctttagat tttttggatc attggctgtt ttggaaagtg
    63421 gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa tggaacatga tctttcggtt
    63481 ttagactatg aaaaagtttc aagtaatcga taccgttcta ttttacaagc agaaaatcga
    63541 ttttatcatg aatatgatca aaggcattta tccagtcacc gacgacaatt attgttaaga
    63601 acagctaaac agttcttgac cgtcaaaaac cggcatatct ggcggatgac tttgcgctca
    63661 tatgttaaga attggaaggt gtgaattcgt gcgttcggtt tgtatagcta cctttaatgg
    63721 aggcagatat ttaagagagc agttagatag tattttatct cagctttcag cagaagatga
    63781 agtagtcatt tctgatgatg ggtcagtaga tgatacatgg gatatattaa ctgaatacgc
    63841 agaaaaagat aagcgaatcc agcttttttc ggggcctcgt caaggattga tcgctaattt
    63901 tggttatgcg atccagcaga cgaaaggaga tctaatcttt ttggcagacc aagatgatgt
    63961 ttggttggag acaaaagtga aaaagatcac tgactatttt gagcagaatc cggatcagct
    64021 agtagtagtt tctgatttaa tcattgtgga cagcgagctg caagagatcc atgattcgta
    64081 tttttcgttt cgaaattcca aagaaggatg gcttaataat gtgataagaa gtcactatat
    64141 tggtgctggt atggctttcc ggtcttcttt aaaacccgat attttgccga tcccgtcaga
    64201 agtgccgatg catgatatgt ggattggttt gctgggagga aagaagactg ggttcctaag
    64261 agaaccgtta actctgtacc gaagacatga tttaaacgcg agtgagattg aaacacattc
    64321 taaattacgg caaaaaattt cttggcgtgt tcatctgatt acggcgttga tcaaacgaaa
    64381 atggtttaaa cgttgataaa agatgtttaa atccttaata tttccatgac aaatataaca
    64441 attgatgaga taataacaaa gtgaaatttg aaatcaaaaa ggagagcaga tatccatgaa
    64501 gggaatcatt cttgctgggg gcagcggaac acgtctgtac cctttaacaa aggcaacatc
    64561 gaaacaattg atgccgattt atgacaaacc aatgatttat tatccaatgt ctactctgat
    64621 gttggcggga atcaaagaaa ttctgattat ttcaacgcca caagatacac cacgtttcaa
    64681 ggaattattt ggaaacggaa atgatttagg actgcagatc gaatatgcag tacaagaaag
    64741 tccagatgga ttggcacaag cgtttatcat tggagaagaa tttattggag atgatagtgt
    64801 ctgcttggta ttaggggaca atatttacta tggtggcggt ctgtctaaaa tgttgcaacg
    64861 agcagcttca aaagatacag gcgcgacagt atttggctat catgtcaatg atccagaacg
    64921 ctttggtgta gtagaatttg atgagaatat gcaagctttg tcgattgaag aaaagcctgc
    64981 tcagccaaaa tcaaattacg ctgtaactgg cctttatttt tatgacaatg aggtaatttc
    65041 tatcgctaaa ggtatcaaac cttcagaacg tggagaacta gagattacgg atgtcaataa
    65101 agcatatttg gataaaggaa aactatccgt agaacttatg ggacgcggat ttgcttggct
    65161 tgatacagga acacatgaat cattgcttga agcatctacc tttatcgaaa caattgaaaa
    65221 acgtcagaac ttaaaagtgg cttgtttgga agaaattgct taccgtatgg gctacatcga
    65281 taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa aatcaatacg gtcaatattt
    65341 attgaatttg gccgctgaat aaggagtcct tacagatgaa agtaacagat acaaaattaa
    65401 cagatgtcaa aatcgtagaa atggacgtct ttggcgatca tcgcggtttc ttcacagaaa
    65461 gttattcaaa agcaaaattt gcagaacatg gattgaaata tgactttgtt caagacaacc
    65521 attcactatc agctgaagct ggtgtccttc gggggcttca tttccaaaaa ggagaagctg
    65581 cacagacaaa attgattcgg gtagtcactg gagcagtttt agatgtagta gtggatatcc
    65641 gcaaaggtag cccgacttat ggtcaatggg acggatttat cttatctgaa cataatcatc
    65701 gacagttgct tgtacctaaa ggatatgccc atggattcgt tactttgaca ccaaatgtga
    65761 acttccttta taagtgtgat aattactata atgcagaagc agatgggggc atcgcatttg
    65821 atgatccaga tttagcgatt gattggccaa ttgatccatc aagagcgatt atgtcggaaa
    65881 aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga aaatccattt gtttacggag
    65941 aaatctaaga attaaggaga aattttctta tgaaaaacat cattgttacc ggaggtgccg
    66001 gattcatcgg ctctaatttc gttcattacg tggtaaacaa tcatccagaa gtgcatgtca
    66061 ctgttttgga taaactgact tatgcaggaa acaaagagaa cttggccgga cttcctagcg
    66121 atcgcgtaga attggtcgta ggagatgttg cagatgcaga attagtggac cgtctagtaa
    66181 aagaagcgga cgcagttgtt cattacgcgg cagaatccca taacgataat tcattaaaag
    66241 atcctttccc atttgtacaa acgaatatca ttggtacgta tacactttta gaagcttgcc
    66301 gcaagtatga tgtacgctat catcacgtat ctactgacga ggtttacggc gatcttccat
    66361 tacgtgaaga cctgccagga catggggaag gcgaaggaga aaaattcact gcggaaacac
    66421 catataatcc ttcaagtcca tattcttcta caaaagctgg ttcagatctg ttagtaaaag
    66481 catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa ctgttcaaac aactatggtc
    66541 cttatcagca tattgaaaaa tttattcctc gccaagtaac gagtatctta agcggtattc
    66601 gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg gatccatacg aatgatcatt
    66661 cttcagcagt gtggctgatt ttgacgaaag gcagaattgg cgaaacctat ctgatcggtg
    66721 cagacggtga agaagataat aagaccgtga tggaattgat cttagaaatg atgggccaac
    66781 caaaagacgc atacgaccat gtaaacgacc gcgcagggca cgatttgcgt tatgcaatcg
    66841 attcgacaaa attgcgagaa gagctaggtt ggcagcctga gtttacgaac ttccgtgatg
    66901 ggttggctga cacgatcaaa tggtacgaag aacatgaaga ctggtggaaa aaagaaaaag
    66961 aggccgttga agcagcatat gccaaaaatg gacaataata cagaggaaat ttcactgtat
    67021 taatggaaaa acacatgtga ggcaggcgct cgcctgtctc ttttttaaaa taaaaatcaa
    67081 ggagcggata accatatgtt tttattaact ggaggaaatg gacaattagg tacagaattg
    67141 cgccatttat tagatgaaaa aaatctgcca tatgtttcaa cagatgcaaa agacttggat
    67201 atcacagatg ctgagaaaac aatggcttat attacagaat taaaacccga tgttattttc
    67261 cattgcgcag cttacacagc agtagataaa gcagaagaag aagcaaaaga attagatgag
    67321 aaaatcaatg tcgatggcac aagaaatgta gcacttgcag caaaagctgt gaatgcacaa
    67381 ttcgtctata tcagtacaga ctatgttttc gatggaaaaa acaaaaatgt agaatacgaa
    67441 gtagacgacc aaacgaatcc actaaatgaa tatggtcgta cgaaattatt aggcgaaaaa
    67501 gcagtagaag agattttaga agactattat atcatacgaa cttcttgggt tttcgggatc
    67561 tatggacaca atttcgtctt cacgatgcaa cgcctagcgg aaacccgcga caaactgact
    67621 gtagtaaatg accagtttgg gcgaccaact tggacacgta ctttggcaga atttatggta
    67681 tatgtcatcg aagaaaaagc accatttggt atttatcatt tgtcgaacga aaatagctgt
    67741 tcttggtatg agttcgcaaa agaaatattg aaagatacag atgtagaagt tgcacctgtt
    67801 acgtcagaag aatttccgca aaaagcaaca cgtccgcagt attcagtcat gagcctgaaa
    67861 aagacagaag cgttaggttt tgtcatacca acttggcaag aagcacttgc gcaaatgctg
    67921 gaagctgcca aaaaaatgaa taagtaattt cctttttatc aaatagaatt tggagcatga
    67981 tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag ccaaaagcga atcaggcaga
    68041 ttcgaatttg gtgcttgacc tgtttctaaa actccgctat cttattggcg ttttagtcat
    68101 cgtggtcatc gttactttta atttaaacgg aagttcactt ggtgtttggg ataaatatgt
    68161 gtcgcaaaga gacgatggga aaaaaactga tgtgattttt ggacagaatc gggaagtgcg
    68221 gtcagacgaa tggctggtgc agacaccttt ttacctctca caagctgaag aggacttttc
    68281 gttggtgaac gaggattatt cattaaacgg acaaaacatg atcattgcgt ataattctcc
    68341 tgtaaaggat attacggtta tcggaaaacc attcaactgg ggattctttt tcctcggaag
    68401 agatcgagga ctttcctttt attgggcgat gaaattagtc gtcatggtct tgcttggatt
    68461 cgaagtactg atgatattaa cgaagaggaa aaaagctcta tcgctaatcg gtgcatttgc
    68521 gctgacattt tctccagcag tccagtggtg gttcatgcaa catgtgggcg atttgatttt
    68581 ctttactttg ggactgatga ttgcttttta tcattatttc tacaatcatg ataaaaaatg
    68641 gctacgaacg ttgatgatgc ttttggtggt tattttcggt attgggttta ttttagtgat
    68701 ttacccagcc catcaagtga tgctggcgta cttgctgatt ttttattttg ttggcctgct
    68761 tatttactat agcaagaaag tttcatggga tctgttggat gtagtgttga ttggaggggc
    68821 agttttattt attggcggag tcatggttca tttctggcta acgtcaaaag acgcattgtt
    68881 agcttctttg aataccttat atccaggaaa acgggtaagt actggaggaa agtggacgat
    68941 tggcgaattt ttttctttcc ttacaaattg gaaaattcct tttgaagata tcaactattc
    69001 gaataactct gaagtggccc tgttctatca cttttttcca actgtattcc tagcaagtcc
    69061 atttgtgtta tttggcaaaa aagatagcga acaaaagtta ctgggacgta ttttgatgat
    69121 tttttgtctg ttttctatct tttggattac cgttgggttg ccaaaatggc tagcagaaat
    69181 cactctgctg tcttatgttc ctccattaag agccatcctt acttttagtt ttgcggcttg
    69241 tttattatcg atctggttta tcgcttatat ttggagagaa gatgttattc ctatttggta
    69301 taaattcatt ttgttggcag taaatgcatg gtcttatttc tatgcgatta cgcattcgaa
    69361 aatggataat tatttttcgg attttgaaac catcgcagtt gctgtgttgg gaacagtgat
    69421 tttagctttt gcattatttg gctggagacg cttgttttac ctcgcttttg tcgccttagt
    69481 tatcgtatct ggctttttcg tcaatccagt cgtagaaggt acaggagcga tttttgaaaa
    69541 aacactggca aaagaaatcc aaaaaatcga tcaacaagat cctggtaaag tttggttgtc
    69601 tgaagatgaa ttatataact tcaccccaac attgggagtg aaatccttca atacagtacg
    69661 cttttatcca gatatagctg catggaagaa aatcgatccg aaagaagaat acgagaagta
    69721 ttataatcgg tatgctcata caagagcttt tatcgctaag gaagatacaa agttcatttt
    69781 agatcgtcca gatatgtttt cggtcacgtt gaattttgaa gacgcagaaa aattagggat
    69841 cgactatgtg atcagtaaac gaccattaac tgattacaac caattatatg aagcgcagtt
    69901 cacgcagcta tatggaccgg ataaagacgg ttatatgatt tttaaagtag aatatccgga
    69961 ttatccagat gtaccacaaa cacaaactca ggaaaatcag ctagtcgtac agtaataggc
    70021 tagctgagaa taatcctgaa atattagtag aaaggagagt gaagataatt caagcaatcg
    70081 tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct tttgttgctg acgctagtct
    70141 tttttgtttg gacagcaggg gtaggttttc cagcaacttt gtttatatat aacaatagtt
    70201 cattacttat cgcttttagt gtggctattt tgctcatttt aaatgtttat aaagcaaaag
    70261 gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt cattttcttt tcttactttc
    70321 gggaattaag aaatgagtct ttatttggag acttactgtt tcctttggtt attttatttt
    70381 gtttagtaat taaatggatc gagtggacaa aatgggatcg cggtatttat ctgattgtct
    70441 ttacggtctt cctcggaatc acgttatatc gtgtatttac agagatccaa gtaccagaag
    70501 gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat acgaatacaa
    70561 tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt tcttttgaac
    70621 gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc agccttttta ggtatttggg
    70681 tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg gatcttttac
    70741 ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ctacacggtt acgattcttt
    70801 tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg tttaccgatc
    70861 gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa caagttttac
    70921 taggaatgaa aacatttatc ttccagcgag gaaaccaatt tttaggaaac cacaatagtt
    70981 ataattctat tttgaatatc tatggattga taggatttgg gattgctgct ttgctgctta
    71041 tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag atgactttta
    71101 tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg acttcatacc
    71161 catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat gatgaaccag
    71221 aagttgcgga ggaattctat gttatggaag aggcatatga cgatatgact tatgatgata
    71281 catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc tatcattgat
    71341 aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc
//
LOCUS       DACDTO010000006        70962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDTO010000006 DACDTO010000000
VERSION     DACDTO010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 70962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.006"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            162..392
                     /locus_tag="IWK70_000414"
     CDS             162..392
                     /locus_tag="IWK70_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9612989.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="IWK70_000415"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="IWK70_000415"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9612990.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="IWK70_000416"
     CDS             1803..1979
                     /locus_tag="IWK70_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9612991.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2114..2410
                     /gene="yidD"
                     /locus_tag="IWK70_000417"
     CDS             2114..2410
                     /gene="yidD"
                     /locus_tag="IWK70_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9612992.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2474..2941
                     /locus_tag="IWK70_000418"
     CDS             2474..2941
                     /locus_tag="IWK70_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9612993.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3026..3910
                     /locus_tag="IWK70_000419"
     CDS             3026..3910
                     /locus_tag="IWK70_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9612994.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4027..4260
                     /locus_tag="IWK70_000420"
     CDS             4027..4260
                     /locus_tag="IWK70_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9612995.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4348..4428
                     /locus_tag="IWK70_000421"
     tRNA            4348..4428
                     /locus_tag="IWK70_000421"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4382..4384,aa:Tyr,seq:gta)
     gene            4435..4506
                     /locus_tag="IWK70_000422"
     tRNA            4435..4506
                     /locus_tag="IWK70_000422"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4467..4469,aa:Gln,seq:ttg)
     gene            5021..5728
                     /locus_tag="IWK70_000423"
     CDS             5021..5728
                     /locus_tag="IWK70_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9612996.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5788..6432)
                     /locus_tag="IWK70_000424"
     CDS             complement(5788..6432)
                     /locus_tag="IWK70_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9612997.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6559..6846
                     /locus_tag="IWK70_000425"
     CDS             6559..6846
                     /locus_tag="IWK70_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9612998.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            6954..7274
                     /locus_tag="IWK70_000426"
     CDS             6954..7274
                     /locus_tag="IWK70_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9612999.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7355..8668
                     /locus_tag="IWK70_000427"
     CDS             7355..8668
                     /locus_tag="IWK70_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9613000.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8665..9384
                     /locus_tag="IWK70_000428"
     CDS             8665..9384
                     /locus_tag="IWK70_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9613001.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9512..10063
                     /gene="raiA"
                     /locus_tag="IWK70_000429"
     CDS             9512..10063
                     /gene="raiA"
                     /locus_tag="IWK70_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9613002.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10285..12819
                     /gene="secA"
                     /locus_tag="IWK70_000430"
     CDS             10285..12819
                     /gene="secA"
                     /locus_tag="IWK70_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9613003.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            12945..14046
                     /gene="prfB"
                     /locus_tag="IWK70_000431"
     CDS             join(12945..13007,13009..14046)
                     /gene="prfB"
                     /locus_tag="IWK70_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9613004.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14151..14837
                     /gene="ftsE"
                     /locus_tag="IWK70_000432"
     CDS             14151..14837
                     /gene="ftsE"
                     /locus_tag="IWK70_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9613005.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14821..15714
                     /locus_tag="IWK70_000433"
     CDS             14821..15714
                     /locus_tag="IWK70_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613006.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15866..17314
                     /gene="cls"
                     /locus_tag="IWK70_000434"
     CDS             15866..17314
                     /gene="cls"
                     /locus_tag="IWK70_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9613007.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17651..18508
                     /locus_tag="IWK70_000435"
     CDS             17651..18508
                     /locus_tag="IWK70_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9613008.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18623..19543
                     /gene="pstC"
                     /locus_tag="IWK70_000436"
     CDS             18623..19543
                     /gene="pstC"
                     /locus_tag="IWK70_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9613009.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19543..20427
                     /gene="pstA"
                     /locus_tag="IWK70_000437"
     CDS             19543..20427
                     /gene="pstA"
                     /locus_tag="IWK70_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9613010.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20442..21248
                     /gene="pstB"
                     /locus_tag="IWK70_000438"
     CDS             20442..21248
                     /gene="pstB"
                     /locus_tag="IWK70_000438"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9613011.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21264..22022
                     /locus_tag="IWK70_000439"
     CDS             21264..22022
                     /locus_tag="IWK70_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9613012.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22035..22712
                     /gene="phoU"
                     /locus_tag="IWK70_000440"
     CDS             22035..22712
                     /gene="phoU"
                     /locus_tag="IWK70_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9613013.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            22950..23981
                     /locus_tag="IWK70_000441"
     CDS             22950..23981
                     /locus_tag="IWK70_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9613014.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            23978..24745
                     /locus_tag="IWK70_000442"
     CDS             23978..24745
                     /locus_tag="IWK70_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9613015.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24845..26413
                     /gene="liaX"
                     /locus_tag="IWK70_000443"
     CDS             24845..26413
                     /gene="liaX"
                     /locus_tag="IWK70_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9613016.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26713..27030
                     /locus_tag="IWK70_000444"
     CDS             26713..27030
                     /locus_tag="IWK70_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9613017.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27030..27380
                     /locus_tag="IWK70_000445"
     CDS             27030..27380
                     /locus_tag="IWK70_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9613018.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27547..28485
                     /locus_tag="IWK70_000446"
     CDS             27547..28485
                     /locus_tag="IWK70_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9613019.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28500..29330
                     /locus_tag="IWK70_000447"
     CDS             28500..29330
                     /locus_tag="IWK70_000447"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9613020.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29374..30399
                     /locus_tag="IWK70_000448"
     CDS             29374..30399
                     /locus_tag="IWK70_000448"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9613021.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30414..31352
                     /gene="galU"
                     /locus_tag="IWK70_000449"
     CDS             30414..31352
                     /gene="galU"
                     /locus_tag="IWK70_000449"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9613022.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31477..31917
                     /locus_tag="IWK70_000450"
     CDS             31477..31917
                     /locus_tag="IWK70_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9613023.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            31920..32522
                     /locus_tag="IWK70_000451"
     CDS             31920..32522
                     /locus_tag="IWK70_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9613024.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32581..33687)
                     /locus_tag="IWK70_000452"
     CDS             complement(32581..33687)
                     /locus_tag="IWK70_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9613025.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            33897..34898
                     /gene="ccpA"
                     /locus_tag="IWK70_000453"
     CDS             33897..34898
                     /gene="ccpA"
                     /locus_tag="IWK70_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9613026.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35235..37580)
                     /locus_tag="IWK70_000454"
     CDS             complement(35235..37580)
                     /locus_tag="IWK70_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9613027.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37859..38761
                     /locus_tag="IWK70_000455"
     CDS             37859..38761
                     /locus_tag="IWK70_000455"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9613028.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            38974..40749
                     /locus_tag="IWK70_000456"
     CDS             38974..40749
                     /locus_tag="IWK70_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9613029.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40815..42401)
                     /locus_tag="IWK70_000457"
     CDS             complement(40815..42401)
                     /locus_tag="IWK70_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9613030.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42652..42876
                     /locus_tag="IWK70_000458"
     CDS             42652..42876
                     /locus_tag="IWK70_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9613031.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43018..44769
                     /locus_tag="IWK70_000459"
     CDS             43018..44769
                     /locus_tag="IWK70_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9613032.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44769..46553
                     /locus_tag="IWK70_000460"
     CDS             44769..46553
                     /locus_tag="IWK70_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613033.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46879..47730
                     /locus_tag="IWK70_000461"
     CDS             46879..47730
                     /locus_tag="IWK70_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9613034.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47784..49535
                     /locus_tag="IWK70_000462"
     CDS             47784..49535
                     /locus_tag="IWK70_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9613035.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49539..49709
                     /locus_tag="IWK70_000463"
     CDS             49539..49709
                     /locus_tag="IWK70_000463"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613036.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49749..50651)
                     /locus_tag="IWK70_000464"
     CDS             complement(49749..50651)
                     /locus_tag="IWK70_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9613037.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            50882..52285
                     /locus_tag="IWK70_000465"
     CDS             50882..52285
                     /locus_tag="IWK70_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9613038.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52285..53718
                     /gene="ascB"
                     /locus_tag="IWK70_000466"
     CDS             52285..53718
                     /gene="ascB"
                     /locus_tag="IWK70_000466"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9613039.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53863..54789
                     /gene="trxB"
                     /locus_tag="IWK70_000467"
     CDS             53863..54789
                     /gene="trxB"
                     /locus_tag="IWK70_000467"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9613040.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55003..56730
                     /locus_tag="IWK70_000468"
     CDS             55003..56730
                     /locus_tag="IWK70_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9613041.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56802..57095
                     /locus_tag="IWK70_000469"
     CDS             56802..57095
                     /locus_tag="IWK70_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9613042.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57187..58047)
                     /locus_tag="IWK70_000470"
     CDS             complement(57187..58047)
                     /locus_tag="IWK70_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9613043.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58139..58567
                     /locus_tag="IWK70_000471"
     CDS             58139..58567
                     /locus_tag="IWK70_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9613044.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58729..58905
                     /locus_tag="IWK70_000472"
     CDS             58729..58905
                     /locus_tag="IWK70_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9613045.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            58932..59378
                     /locus_tag="IWK70_000473"
     CDS             58932..59378
                     /locus_tag="IWK70_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9613046.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IWK70_000474"
     CDS             complement(59532..60911)
                     /gene="brnQ"
                     /locus_tag="IWK70_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9613047.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61617..62588
                     /locus_tag="IWK70_000475"
     CDS             61617..62588
                     /locus_tag="IWK70_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9613048.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62612..64804
                     /locus_tag="IWK70_000476"
     CDS             62612..64804
                     /locus_tag="IWK70_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9613049.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64821..65297
                     /gene="ybeY"
                     /locus_tag="IWK70_000477"
     CDS             64821..65297
                     /gene="ybeY"
                     /locus_tag="IWK70_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9613050.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65275..65676
                     /locus_tag="IWK70_000478"
     CDS             65275..65676
                     /locus_tag="IWK70_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9613051.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65691..66590
                     /locus_tag="IWK70_000479"
     CDS             65691..66590
                     /locus_tag="IWK70_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9613052.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66733..67545
                     /gene="recO"
                     /locus_tag="IWK70_000480"
     CDS             66733..67545
                     /gene="recO"
                     /locus_tag="IWK70_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9613053.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            67929..68846
                     /gene="glyQ"
                     /locus_tag="IWK70_000481"
     CDS             67929..68846
                     /gene="glyQ"
                     /locus_tag="IWK70_000481"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9613054.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68848..70923
                     /locus_tag="IWK70_000482"
     CDS             68848..70923
                     /locus_tag="IWK70_000482"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9613055.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23222 a        12429 c        15179 g        20132 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttttagta aaagaaaaga
     2101 aggaatctaa ttgatgaaga agatattgat ttatttagtt agaggatatc agcgctttat
     2161 ttcgcctgca tttccaccta gctgccgtta ttacccaacc tgttccaatt atatgataaa
     2221 agcaattcaa acccatggtg cactaaaagg gacggtaatg gggactgcac ggatcttacg
     2281 ctgtcatcct tttgtcaaag gcggtatcga ttatgtacca ttgacattcc aattaacgcg
     2341 aaataagaaa gataaaaaag aaccagatta taaaaaggtt gtgaaaaata cgagtagcac
     2401 tgagcgataa agaaaaagag gaaaattatt gtcaaagtaa caactccgca actacttata
     2461 tgggggacaa aaaatgatcg aagaactaaa caagaaagat ttaccatggt ccttgttatt
     2521 agatgcagat cctgataaag aaaaagttca agtatatgtt tccagaggga gtggctggat
     2581 atggaaagaa aaagaaaaaa caataggtgt actgatctat gtggcaaggg aaaaagaata
     2641 cgaaatcgtc aatgtagcag tagccccaac gtatcaagga aaggggattg gcggaaaatt
     2701 acttgagaca gcttttcaga agctttcaca actcgcttcc agtcaaactc agatcatcat
     2761 acgaaccggc agtactagca gtgcggcgtt acatctctat caaaaaatgg gctatgtgga
     2821 gattggtcga gtcaaagact attttatcca tcattactct gagcccattt ttgaaaatgg
     2881 agatcaatta agggatcaag taattttagc aattcactta cctaaaaata gctttaacta
     2941 ataaactttc aattcgagaa aatgctttca aatgctaaat agatacatta caatagcttt
     3001 agcaaacaaa aaggagaata tttttatggg aatattagtt tttgattttg gtggatcggc
     3061 tgtcaaatat ggttgttggg atggcaaaga gattaaaggt aagggacaat ttgcaacgcc
     3121 tgaatcttgg gaagaaatga aagcgcaatt atttcaagtt tataaaaaaa tactcgtttc
     3181 ttttgaagtc gaaggaattg gaatcagctc tcctggagta gtgaataatg agaaacaggt
     3241 gattgaggga atcagtgcga ttccttatat tcatggattt aatattttcc gagatttgga
     3301 aactctgttt caactcccag taacgattga aaatgacgcc aactgtgccg gaatggctga
     3361 attttatgaa ggtgctgccc gtgattatca agatgttgct tttgttgtgg caggaactgg
     3421 gattggcggg gcgttgttca cgaaaggaca aatcaataaa ggggcacatc tttatggtgg
     3481 agaatttggg ttgatgttct taaatggcga taagacattc agtaaacttg gtacagcggt
     3541 acagatggct tggcgttatt gtgaacgcaa aggtctcgat aaaaattctt ataccggaaa
     3601 agatgtcttt gaattagcag aaactggaga tgcaatagca aaagaagaag ttgagagttt
     3661 ttatacgtat cttacaaagg gattattcag tattcaattt tcgtttgatc cagaagtgat
     3721 cgtattaggc ggcggtgttt ctgcaaaaga agggctcatt gatgagatca aaagccgaat
     3781 gaagaagctg acagaacaat tcgatctgca tgatttcgaa ccgcagattt tattatgtga
     3841 atacagaaac gatgctaatc ttgtaggtgc agcagcaaac tatctcgctg tacgtcaagg
     3901 ggagaattaa gagctttaga gaaggtgcag cttgcataaa agaagtgaat gaaatgaata
     3961 ggagattttt acctaaggaa tgttttattt gctgcagtat gataaaatat ctgagaggtg
     4021 aaaatgatgt caaaaaaaaa taaagatgtt gaagtgcgta tagaagaagt gaaaaaagat
     4081 atcaaaggta agaattatga agtcactcag ttatttatag ggaaaaaaat gattggtgag
     4141 atcttagcct atgggccaaa agaatatgaa attttctttg gtgaagaaga ttttggaaaa
     4201 gaaaaaagtc ttgaaaatgc gatagaaaca gtgattcgac actggaatct gcacgaataa
     4261 aaaaatatca aaaaaacttt caataaaggg ttgcaaaaat ctggaaaatt aggtattata
     4321 actaagtcag gttgatacaa acctttcgga ggagtagcga agtggctaaa cgcgacggac
     4381 tgtaaatccg ttccttaggg ttcagtggtt cgaatccact ctcctccatt tcattggggt
     4441 atagccaagc ggtaaggcaa gggactttga ctccctcatg cgttggttcg aatccagcta
     4501 ccccagtata ccacaatcgt aagctctcgt tagttcgaga gctctttttt ttgctttgct
     4561 accttgttcc ttgaagattc tgctgattta agatagagtt aagttaacta caagaattgg
     4621 agggatccac atgaaatgga aagaaaaagt agacgaagca gctgaaaagt tatatgattt
     4681 ggtcaaatca gaaaaataca atattgaagt gaatattccg aaaaaaggcg gaaaagcggt
     4741 ccaggtaaaa tcaaaacgcc caacgaatca tacaaaaaaa tggatggcta aaaaccgcta
     4801 ataataacaa aagatccata agtcggcatt ttcatcgatt ttagggtctt ttgttatttt
     4861 ctaggttgaa catatctaaa aaattgtttc tgacaatagg agtaggaaga acgatgtgtg
     4921 tctaaatcct ctgttgatgg atagaaacct ttatcttttt cagttgtgaa tcaggtatat
     4981 accagttata atagaagaaa cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt
     5041 atatatacag attcatgacc aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg
     5101 agaccgtctt ccttcagaaa gagaattagc agtgaaattc aatgtcagcc gtatgacgtt
     5161 gagacaagca attcaaaact tggcagatga aggaatctta gaaagaaaga tcggttcggg
     5221 tacatatgtt gcgcgagaaa aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga
     5281 aattatggaa tcgcaaaatc gagtaccgtc tagtcggaca atctcttatt tcttgacttc
     5341 gccaagctca agtgaaatgg aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat
     5401 ggaacgtatt cgtttcgcag atgatgtccc tatttgtttt gaagttgcca gtgtgccaca
     5461 aaagctgatc gatggctaca gcaaagcaga gattactgaa tcgttgtatc gcacgctaga
     5521 agaaaaaggc gggaaaaaga tcggtccagc gaatcaaacg attactgctg tgttggcttc
     5581 tgagaaaatt gctgactatt tagaggtaaa acgaggagat gcagttcttc gtttgcgcca
     5641 gatttcttat tttgaagacg gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa
     5701 tcgttttgag ttttatttgg aaaaataaaa aacaggtgat ccctgattcc ttacttagaa
     5761 ataaaagaga atcagaatca cctgttttta tatatgatca gttaccagtt gctcgtgata
     5821 agaaacagga ccctcttcaa attggacttg tccgttcagc agctcaatga tggattcttt
     5881 aaagtggtca gcactttttt catctacagc acagatgacg ctaacttgat cagtatattg
     5941 gatttctttt ataaaaaaat gttggagttc aagataattc tgtaattttc ctagttgcgg
     6001 atacgtgatc atcacagaga tctcctgttg caaggtacct tctacgattc caatgtcaga
     6061 caatgcttga gagacagcat gagtgtatgc tcgtattaat ccgcctgtgc cgagtttcgt
     6121 tcctccaaaa tatcgagtga ctactgcagc tacattgatc agttgatttt ttttgaggac
     6181 ttccaacatc ggtacgccag ccgttccact tggttctcca tcatcgctgc ttcgctggat
     6241 ttcatttttt tctccaatga tgaaggcgct gcagttatgg ttcgctttcc aatgttcttt
     6301 ttttatttct tgaatgaaac tttttgcttg gtcctctgat tctattcgtc taagagaaca
     6361 gataaagcgt gattttttga tctcaatctc actttgtcca ttttctttta ttgtacgata
     6421 gcgctcgatc atcttgtcac ctcttttttt cattttcttt attcagtata aataaagttc
     6481 taggttgttt caacactgta acggttgcgc aatcgctgta gtatgcgata ataaagacaa
     6541 aggtaggtgg atctggggat gcaggaacga atgaaaaagt acgatgcaat tacgcattat
     6601 ttgaagaaca atggcggctc tcaagtaacg ttgaccttta cccaatttga cgaactgctg
     6661 tttcccagta acggacttcc caaaacagca agggaaagta cagattggtg ggcaaatgat
     6721 tataaacacc ctgaaaaagg tgcctatggc tggataaatg caggctatga agtagttgtc
     6781 atcaatttgg acaaagaata tgtcgtgttc aataaattag tgaaatctag ttggctattt
     6841 gattaaataa accgcgttta gaaaacgttg acaaatttct ttctttttat ataatagagt
     6901 aacagacgcg aagaaaacgt ttgcgtagtt tgttttaaaa aggagagaaa agtatggcta
     6961 aaaaaacaat catgcttgta tgttcggctg gtatgagcac aagtttacta gtaacaaaaa
     7021 tgcaaaaagc tgctgaagca aaaggaatcg actcggatat tttcgctgtg tccgcttctg
     7081 acgcagataa caatcttgca aataaagatg tagatgtact gttattagga cctcaagttc
     7141 gctttatgaa agctgatttt gaaaaacgtc tggaaccaaa aggcattcct ttagacgtca
     7201 tcaatatggc tgattatggt atgatgaatg gcgaaaaagt gttagatcaa gcaatcacgc
     7261 tcataggtga ataaatagaa cagtataact atcgaacaag cgatccaaaa aatagatcgc
     7321 ttgttttttc gtatattttt ttagggcgaa taatatgaat catttttggg gtagaaggct
     7381 tctgacgaga gatattaaag caatagactg tgaggaggta aatttaccaa attatgagaa
     7441 gatcgcagca gtggaacgtt tgggcaactg gattctttgt catcgctgtg gaatgaggac
     7501 gtccgtcatt gatggacagc ttgcagatta cggttatttt tgtacacatt gtctatattt
     7561 agggcgatgc gacactcaac aagatttata tttgtttgat cagcctgaag caaaacctcg
     7621 agaagtcgtc tttttttgga caggacagct tactgaaaaa caaacggaaa tcgcaaagag
     7681 aatactttgc cattcagaaa aaagacatca tcttatttgg gctgtaacag gtgcagggaa
     7741 aacagagatg ctttatccta ttttagtgga aactttaaaa gctggaggaa gagtagcgat
     7801 ttgtacaccg cgagtcgatg tttgtaatga attgttttta cgctatcgtt atgtttttcc
     7861 agaagaaaaa atcacgttgc tccatgggaa tacagcaacg gcttatacct ttacttcttt
     7921 tgttatctgt acgattcatc agctataccg tttttatcgg tcctttgatt taatagtgat
     7981 cgatgagatc gatgcgtttc cttataacgg ggatgcagga ttagctcatg ctgtgcaaac
     8041 agcgattaaa ccagacggac gattcatcta cttaagtgcc acacctgata aaaagttact
     8101 aaaggaaatc aagcagacgt ttgacatcca caaacttgct ttgcgctttc atcggcgttt
     8161 acttcctgaa cccgtcttgg ttttcatgaa taattggcga caaaaatgca agaacaagaa
     8221 gaagataaaa tctttggtgc ggatcattca tcaattaatt aaagaaaacc atatcctttt
     8281 attttgtcca agtatcacga tgatgcatcg tttgaaagcc acacttcaag aggctcttcc
     8341 gagatatgag atcacagatg tttcttcaag agacaatcag cgggcggaaa aagtccagaa
     8401 aatgcgagaa gcaaaatatg acatactgtt aaccacgatg atccttgaac gtggagtcac
     8461 atttgaaaga atatccgtta tcgttttagg agcggatcat ccggttttta ctaaatcttc
     8521 tttggtacaa atcgcaggaa gagccgatcg taaagggcct tatacgaaca gtcaagtata
     8581 ctttttctat gaagaaaaaa ctgcagctat tcgaaaagct tgtcaggaaa tcaaagaaat
     8641 gaatgaagaa gggaagaaga tgctgtgagg tgcagttgct gcagtaaaga tatcgtccaa
     8701 aatttaacat tcagcgagat tattggtgtt tcgaaataca gattacctca gacttgttca
     8761 gtttgtcgcg cacaatttca tttattggaa acaagaggct gttctggctg tcaaaaacca
     8821 atccatttca atctagagag tacgaaaaag aaagtaaagt ctattcccgt ttactgcagt
     8881 gactgtcaag aatggaagaa gaaatatccg acctatgatt ttcagcaccg tgcctttttt
     8941 gtgtacgatg aaaatatgaa ggcctggctc aatcaataca agtttatggg agatatccga
     9001 ctagcaggca cctttgcata taaatggacg cagataaaaa aagaataccc gacatacatc
     9061 tattgtccaa tacctttatc tgaaaaaagg cgaacagaaa gaggattcaa tcaagtaagc
     9121 gagatgttga aagcagcaag aattcctttt cagatgctac tcaaaagaga gaagcatctg
     9181 ctggctcaag caaaaaacac gcgaaaggaa agattggaga tgcctcaacc ttttgaactt
     9241 gcagtcacct tttctaagat acaaaataaa aaaatattga ttattgatga tgtctataca
     9301 acaggacaga ctcttttcca cgcagcagat tgtttgcagg tcgcacagcc gaaaatcatc
     9361 cgaaccattt ctttagcaag atgaattaga tgtggaaatt tgatagcgat ttaatttgca
     9421 aataacctag agttcgatat aatagaatta agaagaggga aagagtggtc cttctccttc
     9481 ttgattgtgg tagacgaaag gggtaatttt tatgtttaga tataatgtac gcggcgaaaa
     9541 tatcgaagta actgaagcga ttcgcgacta cgttgagaaa aaagtaggca aactagaacg
     9601 ctatttcagt gacgcacccg atgcaacggc gcatgtgaac ttaaaagttt atacagaaaa
     9661 aacagcaaaa gtggaagtaa cgatcccatt accttatctt gtattacgtg ccgaagaaac
     9721 ttctccagat ttatacgcaa gtatcgattt ggttgttgac aaattggaac gccaaatccg
     9781 caaattcaaa acaaaaatta atcgtaaatc ccgtgaaacg ggcatgaata ctgcagatgc
     9841 tactgcttta ttcaatgaag aggacgaaaa cgaaaatgat tctgaattag atatcgtacg
     9901 tacgaaacgc ctttcattga aaccaatgga tagtgaagaa gcagtcttac aaatgaacat
     9961 gctgggacat aacttcttta tttttgaaga tgcagaaaca aatggtacaa gtatcgttta
    10021 ccgccgtaaa gatggaaaat acggactgat tgaaacagac tgatcagcct tacttaaata
    10081 acaacgaaaa cttgcagcag ctactgacag ctgccgcaag tttttttgag tttcctcctc
    10141 ttttagatga aatatgaata aattatgaag ttcttacttt aatacgctta agatatttca
    10201 attatctata actaatgata aaatgaaaag taaagtctac atagtctgga taatacacag
    10261 aaggaaaagg agattacaca acgcatggcc aatttgattc gttcacttat cgaaaacgat
    10321 aagaaagaac taaaacgttt agatagcatt gcaaaaaaag ttgaagcata cgctgaccaa
    10381 atggcagcat taactgatga acaattacag gcaaaaactg ctgaattcaa gggcagatac
    10441 caaaaaggag aaacattaga tcaattactt ccggaagctt ttgcagtcgt ccgagaagca
    10501 gcaaaacgtg tattggggtt atatccacac cacgtacaat tgatgggtgg aatcgtgctt
    10561 cacgatggaa atatccctga aatgcgtact ggtgaaggta aaaccttgac cgcaacgatg
    10621 ccagtatatc taaatgctct gactggcgaa ggggtacacg tagttaccgt caatgaatat
    10681 ttagcaaccc gtgactcgac ggaaatgggc gaactttata atttcttagg attaacagtg
    10741 gggttgaata tcaactcaaa atcttcggat gaaaaacgcg aggcttataa ttgtgatatc
    10801 acttatagta cgaataatga attaggattt gactacttga gagacaatat ggtcgtttac
    10861 cgccatcaaa tggtacaacg tccgctgaat tatgccattg tcgatgaagt tgactcaatc
    10921 ttgattgatg aagcgagaac gccgctgatc atttcagggc aagcagaaaa atccacagct
    10981 ttatatacaa gagcagacaa cttcgtgaaa cgtttgaaag aagaagaaga ttataaaatc
    11041 gatgtacaat caaaaacaat cagtttaacc gaagcaggga tcgaaaaagc agaagaaaca
    11101 ttcggtttgg ataacttata cgacattgaa aatactgcat tgacccatca cttggatcaa
    11161 gcattgagag ccaactatat catgctttta gatatcgatt atgttgtcca agacggaaaa
    11221 gtactgattg ttgaccagtt cactggtcgg atcatggatg gacgccgtta ttcagatgga
    11281 cttcaccaag caatcgaagc taaagaaggc gtagagatcg aagacgaaac gaaaaccatg
    11341 gcgacgatca ctttccagaa ctatttccgt atgtacaaga aacttgcggg aatgactggt
    11401 acagctaaaa cagaagaaga agaattcaga gaaatttata acatccaagt ataccagatt
    11461 ccgacaaatc gtccggttat ccgtgatgat cgtccagatt tgctttaccc aacattacaa
    11521 agcaagttcc acgctgtcgt gcaagatatc aaagatcgct accgcaaagg tcaacctgtg
    11581 ttagtcggaa ctgttgcggt tgaaacatct gagttattat cagatatgct gaatcaagaa
    11641 cgtattcctc atgaagtgct gaatgcgaaa aatcacttta aagaagcaga gatcatcatg
    11701 aatgctggtc aaaaaggtgc tgtgacgatt gctaccaaca tggctggtcg gggaacagat
    11761 atcaagttag gacttggggt aagagaactt ggtggtctag cagttatcgg gacagaacga
    11821 catgaatctc gtcgtatcga taaccagctt cgaggacgtg caggacgtca aggagaccct
    11881 ggtatgtcac agttctactt gtcattggaa gatgatttga tgaaacgatt cggttcagaa
    11941 cgaatcaaat ctttcttaga tcggatgaag atcggtgagg aagatgctgt tatccaaagc
    12001 aagatgctga cgaaacaagt agaatctgca caaaaacgtg tagaaggaaa taactatgac
    12061 acacgtaaaa acgtcttaca atacgatgat gtgatgcgtg aacaacgtga agtaatctat
    12121 aaacagcgac aagaagtaat catggaagaa aaaagcttat ccaagccact aatgaacatg
    12181 gtcaaacgga caatcagtcg tgtagtagat gcgcatacac aattagagaa agaaaattgg
    12241 aatttggaaa gtatcatcga ttttgccggc agtactcttg tccatgaaga tagtattact
    12301 cttgcagata tcgaaggaaa aacagctcag gagatcaaag agtatctaaa cgaacgagca
    12361 caagaagtat ttaataccaa agcggctcaa ctaaatggtc cagagcaact gttggaattt
    12421 gaaaaagtcg tgatcttgcg tgttgtggac tctaaatgga cagaccatat tgatgcaatg
    12481 gatcaattac gtcagtcaat cggcttacgt gcttatggac agaacaatcc attagtcgaa
    12541 tatcaaacag aaggctataa gatgtttgaa gatatggtag gcgccatcga gtatgaagtc
    12601 actcgaatct ttatgaaagc tgagatccgt caaaacgtac agcgtgaaca ggtttctcaa
    12661 ggaacagctt ctcactcaga agacggagat gccaaagaaa atagtaatac tagcgcgaaa
    12721 aaacagccaa tccatgtgaa caaggtcgga agaaatgatc cttgtccttg tggaagcggg
    12781 aaaaaatata agaactgtca tggaaaaaat gcaaactaat ttctgacagc aggattggtg
    12841 agacaaattc tttttttaag aattcgtctc accttctttc tgttatttgc tgattctgtt
    12901 agactagata taatgaaaaa agtatagtga aagaaggaaa aaacatggaa atatccgaaa
    12961 ttcgaaatct cctgacagaa atgcaggaaa aggtcacaag cttcaggagg tctctttgac
    13021 ttagaccagc tggaagaaga cattgcggaa gcagaagcaa aaatggctga acctggtttt
    13081 tgggatgata gcgaagctgc tcaaaaagtg atcagtgaaa acaatgcaaa taaagaaact
    13141 tatgatcaat tccattctct tgctgaagaa ctggaagaaa tcgaattgat ggtggagatg
    13201 ttgcaggaag aacctgatgc agccatgcaa gaagaagcag aaacacgtat caatgcgcta
    13261 gatgaaaaaa tgaagacata tgaattatct atgttgttag atggtcctta tgatcgaaac
    13321 aacgccatcg ttgaattgca tccaggggct ggcggaacgg agtcccaaga ttggggcagt
    13381 atgcttttac gtatgtatac acgctgggca gagcagcatg ggtatcaagt agagacacta
    13441 gattatcaag caggtgatga agcaggaatc aagagtgtga ccctactgat caaaggacac
    13501 aacgcatttg gttatctgaa atcagaaaaa ggtgttcatc gccttgtacg tatctcgcct
    13561 tttgactcag cgaaacgacg tcatacttct ttttgttcgg tagatgtgat gccagagctt
    13621 gatgaaacga tcgatataga aatcaatcca gatgatttga aaatcgatac gtaccgagca
    13681 agtggtgcag gtggtcagca catcaataaa acagaatctg ctgtacggat cactcacttg
    13741 cctacaggaa cagtagtagc tagtcaggca caacgttcac aattgaaaaa ccgtgagcaa
    13801 gcgatgggga tgttaaaagc taagctttat caattagaag tggaaaagaa agaacaagaa
    13861 gcagctgcac ttcgaggaga acaaaaagaa atcggctggg gttcacagat ccgttcttat
    13921 gttttccatc catattctat ggtaaaagac catcggacaa acgtagagac aggaaatgtc
    13981 caagcagtta tggacggtga ccttgattta tttattgacg gctatttaaa aatgcatcta
    14041 caataagaac ccattgttaa attgaaataa aattgtaaga ttttgcaagc atgttgaaac
    14101 attgccatgc tataatagct aaagactgaa aaagatatgg agagaacgcc atgattgaaa
    14161 tgaaggatgt aatgaagaag tactcaaatg gtacgaccgc tatccgcaat ctctcaattg
    14221 tcatcgaaca aggagagttt gtctatgtgg ttggtccgtc tggagccggt aaatctacat
    14281 tcatcaaatt gatgtatcga gaagaaaaag cttcaaaagg aactttgaac gtcgcaggtc
    14341 atgatctaat agcaatcaaa aaccgcgaag ttccttatct acgcagagaa atcggcgtgg
    14401 tgttccaaga ctataaatta ttaccaagaa aaacagtata tgaaaatgtt gcgtacgcta
    14461 tgcaagttat cggcagaaaa cctcgggata tcaaaaaacg agtaatggaa gtgctggacc
    14521 ttgttggttt gaaacacaaa gttcgagtct ttcctagcga attatccggt ggggaacagc
    14581 aacgtgtctc tattgctcgg gcaatagtga atacgcctaa agtacttatc gcggatgaac
    14641 cgacaggaaa tctggatcca gaaaactcat gggaaatcat gaaattatta gatcggatca
    14701 atgcccaagg aactactgtc gtgatggcta cacataacag tacgatcgta aatacgattc
    14761 ggcaccgagt aattgctatc gaaaacggcc gcattattcg agatcaagcg gaaggggaat
    14821 atggatacga tgattagaac ctttttccgc catttattag aaagtatcaa gagtttgaaa
    14881 cgtaacggct ggatgacagt tgcttcagtc agtgcggtaa cgatcacatt gactttagtc
    14941 ggtgtattca tggcagttat catgaacgca actaaattag ctcaagatat agaaggaaac
    15001 gttgatgtct ctgtattcgt agatatcggt acaaaagacg aagagatgaa aaagctagaa
    15061 aaagaattga atgacttaga tcatgttaaa aaagttgaat tttcaagcaa agaacaggaa
    15121 ttgaaaaaaa tccaaaacga aatgggtgat tcctggaaat tgtttgaagg tgacaataac
    15181 ccgttatatg atgtgtatgt agtaagtgct actgatcctt cttatacaaa acaagtagca
    15241 gaagaagcag cagatttgaa gaatgtttct cgttctgatt acggtggagc ttcctcagat
    15301 cggattttcc aaatcgctgg tgcagttcgg acatggggat tagttgcagc ggcattgctg
    15361 ctgttcgtag cgatgtttct catttcgaac acgattcgga tcacgatttt gtctagacag
    15421 cgtgaaattc aaatcatgcg tttagtcggt gcaaaaaatg gatatattcg ctggccgttt
    15481 ttccttgaag gtggctggat cggtctgatc ggtgcgattc ttccgatttt gatcatcaca
    15541 tttggttatg catggttttt ccaattgatc aatccatcat tattacgttc ccattattca
    15601 ttgattcatc cacaaaatat cgtttggaaa atcaatcttt taatggtcgg cattggtgtg
    15661 atcattggtt cacttggctc gatcatttct atgagacgtt tcttgaaatt ctaataccac
    15721 aatataaata aaatgagaaa gttggacagt ttcgttccag ctttcttttt ttattggcta
    15781 ggagaataga aggaagcggc ttttttccct ttactttaaa aaacaacttg ggtattctta
    15841 agttggtaga agaagaggtg aagccgtgaa aattatcgtt gacaattttt ttacgatcct
    15901 tttgattatg aatattttgc tttcctttat catcgttttt cgagaaagaa aggaaactgc
    15961 tcaaacatgg gcatgggtgc tcgttttgat gttcattcca gttgtaggat ttattttata
    16021 tattttttta ggaagaggga tttccaaaga gaaaatcttt gatttgaaga tccaagggaa
    16081 aatcggtaag aacctggaaa ttgaagaaga caaacaggca ttgatgcgag ggctttatcc
    16141 tcatccgcct actggacaag tagatgtgaa acaattgatc tatatgctca cggtatttga
    16201 aagtacgctc tatacgacta aaaatgaaat cactttattt acagatggtc gtgaaaaatt
    16261 cgatgcgctg attgaagata tcaagcaggc agaagaccat atccacttcc agtattatat
    16321 ttatcgcagt gatgcattag gtgaagaagt tcgagatgca ttgacagagg cggcaagacg
    16381 aggggtaaaa gtcagagtcc ttcttgatgc atggggttcc acacaagttt cttccagctt
    16441 ttttcagaat ttgaaaaaag caggcggaga agtcgcattt ttctttcctc tatttgttcc
    16501 gtatattaat cctcggatca attatcgcaa tcatcgtaaa atcgtagtta ttgatgggaa
    16561 aatcggatat acaggtgggt tcaatgttgg aaatgaatat ctaggattgg taaagaaatt
    16621 tggttattgg cgagacaatc atttgcgtat ttacggagaa gcggtgtata ccttgcaaaa
    16681 ccgttttttg atggactgga attctcagca tacaaaagaa gtaggctatt ctcccaaatt
    16741 ttttccgtcc attcactcta ctggtgatat agctgttcaa atcgtgacaa gcggaccaga
    16801 taccgaacat gaacaaatca aaatgactta tttgaaaatg atttcgttag ccaaacggga
    16861 gatattgatc caaacgcctt attacatacc ggatggatcg attcatgaag cactgaagtt
    16921 ggctcttctt tcaggcgtaa aagttcatat ccaaatacct aacaaaccag atcatcttct
    16981 cgtttactgg gcaacgtatt ctttcgctgc agagctaatc gagtatggag caaggatcga
    17041 aacatatgag aatggattta ttcatgcaaa gacgatgatt atcgatggtg gcattgtttc
    17101 agttggctca gcaaatatcg atgttcgttc atttcgcttg gactttgagg tcaatacact
    17161 ggtttatgat gaacgaatgg cttcacgtgt aagaaaagct ttttttgaag actcgaaaat
    17221 ttctacgcat ctcacaaaag aaatgtatga aaaccgagga ataatcatca agatgaaaga
    17281 aggattagca agattgattt cgccgctcct ttaaaacata aaaaggacat gagaaccatc
    17341 aacagatgga gatctcgtgt ccttttctaa cggggccatt tctgagagta cgtcagaaat
    17401 ggagaaagcg tctcgcatga gcgaaattaa ctttggggta tctcttgttt tttggggaaa
    17461 gcaagaaata agctaatata tgctattaaa agagacacat ttcaaagcca gtatagcata
    17521 gaaactttta atgagatagt tttttttaca attatgagaa tttattttgt tatttaatga
    17581 ttttgattcg ttttcatcaa gtaatgaaaa tgataaaata aacatacttt aggattaaag
    17641 gagatttcta atgaaaaaac ttaaattatt cttaccaata ataggattag ctattctttt
    17701 atcaggctgt tcaaagtgga ttgatcgagg agaatccatt acggctgttg gctcttctgc
    17761 tttacagcct ttagtagaaa cagttgctga aacttaccag acagaaaatc ctggaaaatt
    17821 catcaatgta caagggggcg gaagcggaac tggtttgtct caagtacagt caggagcagt
    17881 cgacatcgga aactctgatt tgtttgcgga agaaaaatcg gggatcgatg cctcagcatt
    17941 agtcgatcat cgagtagcag ttgttgggat cactcctatt gtgaacaagg atattggcgt
    18001 aaaagacatc agtatggaaa acctgaaaaa actatttaca ggcgagatca aaaactggag
    18061 tgaagtcggt ggaaaagatc agccagtagt tatattgaac cgcgcttcag gaagcggaac
    18121 ccgtagcacg tttgaaaaat gggtattgga cggagaaaca gctgttcagg cacaagaaca
    18181 agattcaagc ggaatggtgc gccagatcgt tgccgacaca cccggtgcaa tcagctatgt
    18241 tgctttttct tatgtttccg atgatgtaga tacgttgaat gttaacggtg tagaaccgac
    18301 agatgaaaat gtgacaacga acaaatggac gatttgggca tatgagcata tgtacacaag
    18361 agatcatcca aaaaaactga caaaagaatt tttagactat atcttatctg atgaagtaca
    18421 agataacatc gttggagaac taggttatat ccctgtttct catatgaaag tcgaacgtga
    18481 ttggcaagga aatatcgtta aagagtaatc tttacacaaa atttacaaac ttagctaaac
    18541 ttttacatgt tattaaaacc aaattgatgt tcaatttgta cactgagtga gtatgttaaa
    18601 cctgattaag ggggagtcat ctgtggaaga tgtgaaaaaa gtattattaa caaaatcaaa
    18661 acgttcaaaa atggaacagc gtggaaaatt catcagtttt ttatgtatcg cgctgatcgt
    18721 gttggttgta ttatctattt tttattttgt agctagtaaa ggattggcca ccttttttac
    18781 tgataagatc aatgtatttg attttctgtt tggtacagat tggaatccaa gccaaatcgg
    18841 agcaaatggg aaaccaatgg taggggcatt gcctatgatc acaggttcgt ttattgtgac
    18901 ctttttatct gctatcgttg ccacgccatt tgccattggt gcagcagtat tcatgacgga
    18961 gatctctcct aaaaacggaa cgaagatctt acagccggtc atcgagctgc ttgtaggtat
    19021 tccttctgtc gtttatggtt ttatcggttt atcagttatc gtgccttttg tcagaagtat
    19081 ttttggcggg actgggtttg gtatcttagc tggtacattt gtgttgttcg ttatgatctt
    19141 gccgactgtc acaacgatga ctgttgatgc attaaaggca gtgccacggc actatcggga
    19201 agcttcatta gcgcttggtg ccacaagatg gcaaacaatc tacaaagtcg tgctacgagc
    19261 agccgtgccc gggattttaa cagcagtcgt ttttggtatg gctcgtgcgt ttggtgaagc
    19321 cttagctatc caaatggtca tcggtaatgc agcattgatg ccaacaggtc ttactactcc
    19381 tgcttctaca ttgacgtcca tcttaacaat gggaatcgga aatacaatca tgggaacagt
    19441 tgaaaataat gtgttatggt cattagcgct gatcttgcta ttgatgtcat tattctttaa
    19501 tatcgtgatc cgtatgattg ggaagaaagg agagctgaaa taatgaacgc aaaacgctca
    19561 gataaaattg ctacaggtgt tttatatata gtttcgggga tcatcgtatt gattttagca
    19621 gctttactac tttatatctt agttcgggga ataccgcaca tttcatggtc cttcttgacc
    19681 gaaccttcca aagcctacca agaaggcggt ggaatcggga tccagctatt taactcgatt
    19741 tatttgcttt tgatcacgat gatcatcagt tttcctattt ctttaggtgc gggtatctat
    19801 ctttcagaat atgctggaaa aaactggcta acagatgtga ttcgcacgtc gattgaaatc
    19861 ttgagctcgt tgccttctgt cgttgtcggt ttattcggtt tcttggtttt cgttattcaa
    19921 ttccagtatg gtttctcgat tatttctggt gcattggctt tgactgtatt caacttgcct
    19981 cttttgacta gaacagttga ggagtcttta caagcggtcc attacacaca acgagaagca
    20041 ggattggcct taggtttgtc acgctgggaa acagtgataa aagtggtggt tccagaagcg
    20101 acaccaggga ttttgactgg tgtgatccta agttctggaa gaatttttgg tgaagcagct
    20161 gcattgatct atacggctgg acaaagtgca cctgcgttag attttacgaa ctggaatcca
    20221 ttaagtgttt caagcccgat cagcattttc cgtcaagctg aaacattagc tgtccatatt
    20281 tggaagatca atacagaagg cacgatgcct gatggtgttt ctgtttcggc tggcgcttct
    20341 gccgtgttga ttattgctgt tttactgttc aacttcggcg ctcgtgcgat tggcaaacga
    20401 ttataccgaa aaatgacttc agcctaagga gaagaagcaa aatgaaaaaa tataactgga
    20461 atgaaacaaa tgtcatcacc atgccaaaag agaaagaagt agcactatat acggatgatc
    20521 tacatgtctg gtatggcgac aatgaagcaa tcaaaggagt ggatctggaa tttgaaaaaa
    20581 ataagatcac tgcactgatt ggtccttccg gctgcggaaa atctacgtat cttcgttcgt
    20641 tgaatcgaat gaatgatggg atcgctaata cgaaaatcac tgggaaaatc atgtatcaag
    20701 gcgtagatat taatgcgcct caagtggatg tttatgagat gcgcaaacgg attggcatgg
    20761 tttttcaacg tcctaatccg ttcagcaagt cgatttatga caatattaca tttgctttga
    20821 aacagcacgg cgaaaaaaac aaaaagaaat tagatgaaat cgttgaaaca agcttgaaac
    20881 aagctgcctt gtgggaccaa gtaaaagaca gcttggacaa aagtgccttg gctttatctg
    20941 gcgggcaaca gcaacgttta tgtattgctc gtgcgatcgc gatgaaacct gatatcttgc
    21001 tgctagacga gccggcaagt gctttggacc ctatttcgac aggaacggtt gaagaaacac
    21061 ttgtaaactt gaaagaagac tatacgatca tcatcgtgac gcataacatg caacaagcag
    21121 ctcgtatcag cgactatact gcctttttct atttaggaaa agtaatggaa tatgacaaaa
    21181 ctaacaagat atttaccaga ccaaaaattc aggcaaccga agattatgtg tccggtcatt
    21241 ttggttaaga cggaggcctc aacatgacaa aagagattat ttcatctaaa gatcttcatc
    21301 tgtattatgg aaagaaagaa gcactaaaag ggatcgatct aagcttcaat caaggggaaa
    21361 tcactgcgat gatcggtcct tctggttgcg gaaaatccac ttatctgcgt tctttgaacc
    21421 ggatgaatga cctgattccg aatgtaacga tcaccggaag tgttttatat aaagacaaag
    21481 acatctatgg tccaaaaaca gatgttgttg aactaagaaa agaaatcggc atggtattcc
    21541 agcagccaaa cccatttcct ttttctatct atgaaaatgt catctatgga ttgaaattaa
    21601 aaggggaaaa agataaaaaa gtccttgatc aagtagttga agaaagcctg aaggcagcgt
    21661 ctgtttggga tgatgtgaaa gacaaattgc ataaaagtgc tttatcttta tccggtgggc
    21721 agcagcagcg tgtatgtatc gcacgtgttt tggcagttaa tccggaaatc atcttattgg
    21781 atgaaccaac aagtgcgctt gatcctgttt ctactgggaa aatcgaaagt atgctgttag
    21841 aattaagaga gcggtatacg atgatcatcg tgacccataa tatgtctcaa gcttcacgta
    21901 tttcagataa gacggcattc tttttagatg gtcacttgat cgaatacaat gatacgaaga
    21961 agatattcat gaatccagaa aaacaagaga cagaagatta tatttcaggt cgatttggat
    22021 aaggaggaaa cggaatgcta cgtacacaat ttgaagaaga attgctgaat ctccataatc
    22081 agttctacga aatggggatg atggtgagca gcgccgtgca caagtcagtt cgtgcataca
    22141 tcaaacacga taaaatctta gcccaagaag ttatcgaaaa cgatataaat atcaataaca
    22201 tggaaacacg cttggagaag aaaagttttg aaatgatcgc attgcaacag ccagttacaa
    22261 ctgatttgcg tatgatcatt acggtcatga aagctagctc ggatttagaa cgtatggccg
    22321 atcatgcggt gtctgttgct aaatccacga ttcgcgtaaa agggcagacg agaataccgg
    22381 aaatcgaaaa agagatctcc gatatgtccg attatgtgaa aaaaatggtg gacaacgtcc
    22441 tagttgctta tgtcaaaaca gacgaaaaag acgcacgtac gatcgccaat atggatcaaa
    22501 gagtcaatga ctatttcaat cgaatctata atgcaacaat caaaaatatg caggaaaatc
    22561 ctgagacagt catcagtgga acagactact tgaatgttgc cagttatcta gaacggatcg
    22621 gagactatgt gacaaacatc tgtgaatgga tcgtctactt ggctacaggg aaaatcactg
    22681 agttaaatac gaaccacaat gagattttct aaactgaagc tgtgattatg ccgtttgttt
    22741 aggaaaagag gttgtgaaat cagcggttgt gaaaaagctg ttctgcatca agtaataaga
    22801 acagtcaaac aaaaaatagc tcctcatatt tttcgtttgg ctggacttat taccgcagat
    22861 gcaagctttt gaacaccgtt tattcgggaa agggtttgtg acaaaacatg tcacaaaccc
    22921 ttttcatcat ttcatagaaa ggataaagaa tgcaaaaaaa aattagcatc atcactatat
    22981 tttttatttg tgtgcttact tcatgggtta ttcagcatca agccgcttta tatgaagtac
    23041 ctgtcatcga gatagaaaaa gtacgaataa aagaggatca tcaattgatt acaggaaaac
    23101 tgatgaacac ggatcaaaaa ggagaatcga tttatttatc ggttccttat caaaaaaatc
    23161 aaatagaaac agcaaatata aaagcgagac aaatgtatct agtccaaatg catggagatg
    23221 aatggcagct agtagcaaaa aaaaatgatg gctggctttt cttcttcaca agtatcttca
    23281 ttggcgccat gttgcttatt ggtggaaaac agggtctatt tgctttagga agtctcatca
    23341 tcaatatcgt tttactttta cttttgttgc tcatctttac taaaacagaa gggatttctc
    23401 tattaaatat atctatcctg ttcgtcgtta tagggatgat tattagcata ttggctttag
    23461 atggatggaa tagaagcagt ataatcaaaa tttcagctgc agttacgagt gtttttttag
    23521 cattttttat ttgtcttttg acaatgaatc attggaaaga ccaaggactg cgctatgagg
    23581 aactgaatta tctgacacga ccgtatcgtt ctgtttttct ttctagcgta ctgattggaa
    23641 cagctggagg aacactcgat accgttatca cagtcattgc gacattagaa gaattgacta
    23701 agcgacaacc agaaataaca gcgaaacagt tatggctttc aggaaggcaa gtgggtcgag
    23761 atgtgattgg ctcgatgacc aatatccttt tgttcgtcta tatcagtggg tcagttccat
    23821 cgcttctttt atatttagga aatcaatggt cgttcaaaga aacgatagaa cagcatttat
    23881 cattggaatt tttacgcgtc attgctggca gcttaggaat agtattatcg atccctatct
    23941 cggtcctctt ttttctgact gtaaggaggc tgaaacgatg aatgtactta ttatacttgt
    24001 tgggatttta gcaatcagcg tgctacttat tggcggtacg caaggtatgt atattctttt
    24061 aggcttattc atcaatttag ggatcttttt cctgttgtta ttcggttatc atcagaagtg
    24121 gccgatactg gcattgtcgg tcataggatt tttattgatt gctgtggtta tcttattttt
    24181 tatcaacggt tataatctaa aaatgcgtgc agcttttgtg agtatactga tctttttatt
    24241 ttgtttccta ctattgcttc ctatcactga ttttttagca atccaaggtt ttacatcggt
    24301 agaactagaa gaattatctg gcttagataa gactttagcg attgatttta gattacttac
    24361 gagatcgttg cttctgatta gtcttagtgg tgctgtttta gatgcgagtg tagcaatttc
    24421 tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta tctttttacc aattgcgcca
    24481 ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca gtaatgacac ttctttttgc
    24541 attcatggga agttctttag cgttgatcct ctggtttatt gatttagaca ttccttttca
    24601 gcaagtgatc aatgaaaaat catttgttct agaatatacg atggctattc ttaccacatt
    24661 gtccgcacta cttgttcttc ctttaacctg cgtgactaca gcatatctct ttacaaaaaa
    24721 gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag tcctatgaca tttttactga
    24781 atcagtgcat aatatggatg taaggaaaag cttgcaaaca attttgctta aggaggcaat
    24841 ttccatgaaa gaaagagaac gtattttaga tttagtgaaa aaaggaatcc tttcgactga
    24901 agaaggctta gatttattag aaagcatggc aactgaaaaa gacgaaaagc aaatcaaaaa
    24961 agaagcagac aaggtaactg ctagtcataa agaaaaagat caggcaagtc aattgatcga
    25021 taaacttgaa aacggcgaag aagaaatttc tgagccagtt gatccaaagg aaagagaaag
    25081 acaagatcaa gaaaacttag aaaagatctt agatgaacta gcaactgaag cgaataaaac
    25141 ttcggcgcgt ctagatgaag taaatgctca attcgctgac aataaggcag cacgcaatga
    25201 gaaacaggaa gcattgatgc agctgaatac aaaagaagaa ttaggcgagt tgacagaaga
    25261 agaattagca cagcgtcaga cgttagaagc agaaatcaaa gaattagaag ctagtggcga
    25321 tacactgtta gaagaacaat tgaaattaga agcagaatta aaagatatcc gtaagaatca
    25381 atggtctgag aaaaaagaaa cgtttactga taagtttgaa ttgccagatg attggaaaga
    25441 tcaagcaaca gatacactga atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt
    25501 aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa acagtgaatg acaacatgga
    25561 atggaaagat gtcagcttac gtgtccctgg gattgcgaca acgaaatttg agcatgaatt
    25621 ttactatgaa gctcctgcag caagtatcct tgatatcaag gcagcaaacg gaaacgtgac
    25681 gttaaaaacg tgggacagca acgatgtgaa agttgaagct aagatcaaac tttacggaaa
    25741 aatgggtgct gaaccatttg aagcattctc tgaacggagc cagattgagg ttaatgaaga
    25801 tcatatctcc ttccaaattc caaataaacg tgtgcgtgca gaccttgttt tctacttacc
    25861 taaacgagta tacgaccacg cagctattaa acttctaaat ggcaacatca tgattgaaac
    25921 attggaagct aaagatattt atacaaaatc tacgaatggt aacatcatcg tggaccaatt
    25981 aactgcaacg atgctggaag tagaaggtgt aaacggcaat atcgatattc gaaacgggaa
    26041 catcttagat tctattatcg aaacagtaaa tggtacagtg acatttggtg cgacagcaga
    26101 aaacctttct gtgtctttgg taaatggtga tgtacgtctt acgattaaag aagataattt
    26161 gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa gtcgctttac cagatggcat
    26221 cggtttggaa ggacatgcta aaacaagtct tggaagtatc aacagccgtc tgtccaatta
    26281 cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc
    26341 agatggtgaa atcgcgcaag tgcaactttc aacgacaaca ggaagtatct atttaaaaga
    26401 tacagataaa taaagaggtt gtgaaagaga tgttcagcct tgagcaataa gaagcgattt
    26461 tgtaaaatag cttcccctat tttacaaaat tgtgccttat tgccgatagt ctatctcttg
    26521 aacaccgttt accaagagat tatgaacctg ccaattatcg ccgagtcaca aggaataatt
    26581 tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg tgaccgagaa ttttgcagaa
    26641 agacaccgat taagaagaag gttgtcacaa cgattttctt catttaaaat agtagaacag
    26701 aggtgagaca tgatgaaaaa aaagttgaca aaatctaata aaaatgttgt tttgacaggg
    26761 actttagcag gaataggaga atacatcggg attgatccga ctgttatccg tgtgctttat
    26821 gtattcctga gcttagttgc ttttggaagt ccaattattt tatacatttt attagctttg
    26881 attatcccat ctgcgcgttc tgcacgaaca aactatggac ataacaatgc atattatgaa
    26941 aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa aacgcaaaga agcggaaaaa
    27001 atcgatgatg acgaatggag tgacttttaa tgtcgtactt tcaacgtatt ttagttaata
    27061 cgttaacatt catatcattg gctgttcttt ttcctgcgat ggtccatgtg agcagtattt
    27121 ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa tatgtttgtc aaaccgattt
    27181 tgacgatact gtccctgccc tttacattag tcacattggg attgtttacg tttgttatca
    27241 atgcagcgat tcttaaaatg acctcctttt ttgtgggagc agaacatttt agtttttctt
    27301 cattcggtgc agcgttgtta gtagctatct tgatgtcgct tgtcaatgta attgtcagcg
    27361 aacataacag tagaaaataa aagaaagctg tgccgaacta tcggcgcagt ttttttattt
    27421 agaatcagga atgcgttttc tattacattg gtaattaaat aaaaacgttt tttttaattg
    27481 gtagtacttt tggctagaca atgataaaat gatagagaac actctaatag aaatgaggta
    27541 gcatgaatgt cagaagttgt aaaagttagc caattagtga aaaaattatc tttggaaatc
    27601 gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa ctggggacat ctcacgccca
    27661 ggactagagt tgactggtta ttttaattat tattcccata atcgtttaca gctatttggc
    27721 agtaaagaaa tcagttttgc ggaacggatg atcccagaag agcgtttgat catcatgaga
    27781 agattatgta gtgaagatat gcccgcattt attatttcaa gaggattacc agctccagaa
    27841 gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac tgcgttctcc catctcaact
    27901 tctcgtttat taggagaatt atccagttat ttagatggtc gccttgcccc tcgaacaagt
    27961 gtccacggtg ttttagtgga tgtttacgga ttaggtgtac tgattcaagg tgacagtggg
    28021 ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg gccatcgatt gatcgcagac
    28081 gatcgagtag atgtttatca gcaagatgaa ttaacggtga tcggagaacc gcctaaaatc
    28141 ctggaacatc tgattgaaat ccgaggaatc gggatcattg atgtaatgaa tctttttggt
    28201 gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg tttatctgga agcatgggca
    28261 aaagataaga aatacgaccg tctgggctct gaagacacga ccgttgaaat cgcggaagtg
    28321 ggcattccac aaataaaaat ccctgtcaaa acaggacgaa acgttgcaat catcattgaa
    28381 gtagccgcga tgaatttccg cgcacgtaca atgggctttg acgcaacgaa aacatttgaa
    28441 gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg actagcaagg agtgacaata
    28501 tgttagcaca aatcaatcga gttgcttttg agcttgtagg gattcctatc tactggtatg
    28561 cattgatcat cgtatcaggt atcattatcg ctatgtggct tagcagcaga gaggctgttc
    28621 gggtaggatt aaaagcagat gatgtgacag attttatgtt gtggggattg cctatctcca
    28681 ttattggcgc tcgcttatat tatgttttat ttgatcttcc ccagtatata gctgatccaa
    28741 tccaaatatt caatatccgt tcaggaggct tagctattta tggcggactg attgcaggcg
    28801 cgatcacgct gtattttttt actaaatatc attttatttc tttgtggact tttttagata
    28861 tcgctgcacc aagtgtatta ttagcacagg caatcggtcg ttggggaaat ttcatgaacc
    28921 acgaagcata tggaccagac acgacgcgaa gcttcttaga aagtctgcat ttaccgcaat
    28981 tcatcattga taatatgtat atcgaaggga tctatcgtca accaacattt ctttatgaat
    29041 cactttggag tttgattgga tttattttgc ttttgttttt acgaaagaaa ccacaccttt
    29101 tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta tagtttcggg cgtttcttta
    29161 ttgaaggatt acgtacagac agtctttact ttttaggtgt gatccgtatt tctcaattat
    29221 tatctgctgt gttatttgtc ggtacattga ttctatttat ttggcgcaga aaaaaaggaa
    29281 atttaccgga ttatgaccgc tcattaggaa aaaatcaaca aattatctga tttttagagc
    29341 gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt atagagtagc ggttttagga
    29401 ccaggctctt ggggtactgc tttggcacaa gtcctcgccg aaaacggaca tgacgttcgg
    29461 atttggggac atcgtgcttc acaagtagaa gaaatcaaca cccaacacac gaatcaacgt
    29521 tatttaccag atcattatct acccgattcc atccaagcat tcaccaatat ggaagatgca
    29581 gtaaaagacg cagatgcggt gctattcgtc attccaacga aagcaatacg ttctgttgca
    29641 aaagaactag tgcctaaatt gaatacaaag ccagtgatca tccatgcaag taaaggatta
    29701 gaacagggaa cacataaaag gatctctgac atcttgttag aggaaatccc agcagaaaaa
    29761 agaaaagctg ttgttgttct ttctggacca agccatgcag aagaagtagc aaaacatgac
    29821 atcactacga tcacagctgc aagtacagat gcaaaagcag caaaatatgt acaagagctg
    29881 ttcatgaata attattttcg gatctatacc aatcctgatg tgatcggtgt agagatgggg
    29941 gcagctttga aaaatattat tgctattggt gctggcgcta ttcacggctt agggtttggc
    30001 gataatgcaa aggcagcaat catgacgcgc ggattagctg agatcagccg tttaggtgta
    30061 gcaatgggtg ccaacccgct gacctttatc ggattaagcg gaataggcga tctagtcgtt
    30121 acttgcacaa gtgttcattc taggaactgg cgcgcaggca acttgttagg acaagggcag
    30181 cccctagaag aggtgctagc caatatggga atggtcgttg aaggtgtgaa tactacccaa
    30241 gcagcgatgg aactagccca aatgatggaa atcgaaatgc ccatcactca aacaatctat
    30301 gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa aagaaatcat gttacgtgat
    30361 ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa ggagcgaatg tatatgaaag
    30421 ttaaaaaagc agttattcca gcagcaggat taggaacaag atttttacca gcaacaaaag
    30481 ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac aatccagttc atcgttgaag
    30541 aagcgttgaa atcaggtatc gaagacatct tgatcgttac aggaaaagct aaacgaccaa
    30601 tcgaagatca ctttgattca aatattgaat tagaaacaaa cctaagtgaa aaaggaaaga
    30661 ccgaattatt gaaattagta gaagaaacga cagatgtcaa cttgcatttc atccgtcaat
    30721 ctcatccaaa gggtttagga catgccgttc ttcaagcaag agcctttatt ggaaatgaac
    30781 catttgtcgt tatgctaggc gatgatataa tggaagacga agtaccattg acacagcaat
    30841 taatcgatga ttacgaacgt actcatgctt ctacgatcgc cgttatgcaa gttcctcatg
    30901 atgaaacttc aaaatacgga attatcaatc caggagaagt attggaagat gggttataca
    30961 atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc accaagtgat ttagctatta
    31021 ttggacgtta cttattgact ccagaaattt ttgatgtatt gtccgagcaa caaccaggag
    31081 ccggaaatga aatccaacta actgacgcaa tcgatacatt gaataaaaca caacgcgtat
    31141 ttgcccgtaa attcacaggt aaacgttacg atgtcggtga caaatttggt tttatgaaaa
    31201 caagtattga atatggtcta acacatccag aagtaggtgc acctttacgt gagtacatcc
    31261 tgtctttagg taaaacatta gaagcagaag gaagcaagat cgatcataat ccaaaaggaa
    31321 taacagaaat caaagaagaa aagaaaaaat aacccttttt tctaacgagc ttcagctgct
    31381 aattgaataa aaagtagttg aagctcgttt ttatttgttt aaaaaacacg aaaatgctat
    31441 ccttatagaa aagagagaca agaagggagt ttgatcatgt caggaggaga aattgcagga
    31501 ttgatcgcag caatcgcctt tgcgatactt gtcgttttta tcgttcgtgt gttgttgcaa
    31561 gtttctaaaa ctttagaaaa agtagatcag actgtagcag aagcaaatac gacgatcgaa
    31621 atcgtcacaa aagacgtaga tcttttgtct agacaagtag aaggcttact ggtcaaaagt
    31681 aatgaactgc tcttggatat caatggaaaa gtcgcaacga tcgatccatt gttcacagca
    31741 gttgctgatt taagtgaaag tgtatcagaa cttaatcatt ctagtaaaaa catcgcgacg
    31801 aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg ttgccggaaa agtcggtggg
    31861 acagcgatga aattttttaa aaacaaaaaa catactgaaa cgacaggagg aaaataaaaa
    31921 tggctaaaaa aggtggattt ttattaggtg cagtagtcgg aggcacagcc gcagcaatcg
    31981 ccgcgttatt gttagcacca aaatctggta aagaacttcg ggatgaatta gctgcccaag
    32041 cggatgattt gatggatatg gcttcagatt acacagatat ggcgaaagaa aaatcgactg
    32101 agttaacagg tattgcaaaa gaaaaagcgc aagatctttc tcaacaagca ggtgatctag
    32161 caggtagtgt caaacaaaaa gtaagtgaat cagcagatga agcaggaagt gaatctgacg
    32221 atatcttgtt aaatcttaaa gaacaatcag ctgacttatc taatcgtttc aaaaaaacgg
    32281 cagaagatat gaaaaaacct gcagatgaat taggaagtat tgctaaagat acttcagaag
    32341 atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg agatactgtc aatgaaggaa
    32401 tgtcagaagc aaaaccagtt gcagatgact caaaagaaat gatgaaaatg gcaaaagaag
    32461 atgcgaaaga agctaaaaaa gaaataaaag atacaatgac agattcatct acaaatcact
    32521 aaaatcacaa atttatagaa taaaaaggtg ggagcggact agctctcacc ttttttactc
    32581 ttatttttct aaaacaagta attcttttgg tgtagtcgtg aatggcacac agccatcgct
    32641 tgtcacatat acacagtctt cgatacgtac ccctactttt tcaggaatat agattcctgg
    32701 ttcaagagag aagcacatcc cttcttcgat tactaagtca tttccttcta caagggaagg
    32761 gaattcgtgg acggttgttc cgatcccgtg acctaaacgg tgattgaaat attcgccgta
    32821 gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt tcacctgctg tcacacctgg
    32881 acggacggct tcttgtgcgg caagctgtgc ttcaagagtg atgttgtaga ttttttcttg
    32941 gaagtcggtt ggtttttgat aagcaattgt acgagtagca tcactgcaat agccattcca
    33001 aacaacgcca agatcgaata acacaagttc attcggtgaa atcttcgtat ttcctggtgt
    33061 accatgaggg cttgcagcat ttgctcctgc aaggactaat gtgtcgaaag acatttgtga
    33121 aacaccttgt tttttcaatt ggtattcaat ttctgcaatg atcgcttgtt cagtcacgcc
    33181 ttctttcact gctttaaagc caatctcaaa tgcaacatcg gcccatttac cagcttcgat
    33241 cagtttgtct ttttcctcta gagttttgat cagttgtaga cgttggatga tggaagttag
    33301 atcagtgtaa gaatccgttc ccgtcaagat atcagagata gcatcgaagc gctcgacagt
    33361 caaggcgttc ttttcaatcc ctacattgcc aggctgacca tagcgagcac gaagttcttt
    33421 tccgatgatc tcccaagggt tttcgctgtc taaatagcca cggacatcat acgcccaact
    33481 actgttttga gcatcttcaa cttccaaagc aggagtgaaa aggaaaggat cttgttcaag
    33541 agggataaaa agagctagta cacgctcatg aggatcactt tcgtatccgg aaaagtaagc
    33601 gatgtggcca gggtcgctga tataagccaa ttcgacttgg tgttgtgcca tccattgacg
    33661 tagttcattg atttttacgt tattcatttg gcaacctact ttctaaatta attttgtatt
    33721 caataattgt accactgatt tattaaaaga ccaacgttct aaaaactgaa aaaagtaaaa
    33781 attttcatga aaacagagat aaaacggttg caattcttga gattatttga tattctattt
    33841 tagaaatgta agacgtattt tcagaaaaca tataaaatat gaataggaga acacaaatgg
    33901 aaaagcaaac aatcactatc tatgatgtag cgagagaggc aaatgtttcg atggctactg
    33961 tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc aacaagaaaa aaagtcttgg
    34021 aagttatcga tcgcttggat tatcgtccaa acgcagttgc gcgtggcttg gcaagtaaga
    34081 aaacaacaac agttggtgta atcatccctg atgtcagcaa tatgttcttt gcttctttag
    34141 cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa tattatctta gcaaattcag
    34201 atggcaacga ccaaaaagaa gtcaatgtat tgaataactt actggctaag caagtggacg
    34261 gcgtgatctt catgggtcac catatcacag acgaaatccg tggagaattt tcacgttcaa
    34321 aaactcctgt tgtattagct ggctctattg atccagatga acaagtaggt agcgtaaaca
    34381 ttgattatgt atccgcaaca aaagatgcag taaacaaatt ggcaaaaagc ggcaataaaa
    34441 aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa tggacaaaat cgtctgaaag
    34501 gctataaaga agcattaagc gaaaataacc taccatatag cgaaggattg atctttgaag
    34561 cacaatacaa cttcaaagat ggtctatctc tagtcgatcg gatccataat agcggtgcaa
    34621 cagctgtcta tgtttcagat gatgaattag ccatcggtat tttggatggt ttattagacc
    34681 gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag taacaattca ctactgacag
    34741 aagtagctcg tcctcgttta acaagtatca cacaaccatt atacgattta ggggcggtat
    34801 ctatgcgcct attgacgaaa atgatgaata aagaagaagt cgaagaaaaa acgattatct
    34861 tgccttatgg aatcgaagaa aaaggatcta caaaataata aaaggacaac cattcagagg
    34921 ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat ttcaaaaaac agcatctcat
    34981 gtttgatgaa tattgtgact cgtgattcgg agttggcagg agaacaccgt ttatttggga
    35041 aaaggggtta tgacaaaatt ttgttataat cctttttttg tgtcttaaaa aagagagaaa
    35101 gtggttgaaa ttacatttat cgtatataaa acaatccgtg tatagatgct gcattttgtt
    35161 caccaccatc aaaaaaataa gggactgtga caatactatt gtcacaatcc cttattactg
    35221 tgtattctat taaatcaatc atctttctca tctgattctt tagttgcttg attcgctctt
    35281 gcataagctg aagcggtgtt ccagtaatct gtatagtttt catcggtacc gccaacgcca
    35341 aatttaaagg cgcttttctt cggtccattt ttcgcccata gcgattcaac tgtttttcct
    35401 ggtgtttgga tggatttttt attgacagta acttttccag gtgtttgacc agtaaatcca
    35461 gatacttttt tcttcatcac atcagatgat aattcgaatt tctcatcagt gccaaaaatg
    35521 tcaggttttg cttggtaagc ttgattgatc agattggcaa gataattagc cgaacgtatc
    35581 cttgctttgc tgtccattcc agtattgtca tcgtgccctg cccagctact gatggtaata
    35641 gacggagtag agacaattag ccatgaatct cggtattcat tggtagaacc agtttttccg
    35701 acccaatctg cttttcctag atttccgttt aagcttgaga tggcatcttt gaatggtgta
    35761 gtgatttttg cattgatcac actacgcatc atgtcattca tgatcgatgc cgttgcttcc
    35821 gaatagatcc ggacagagtt gctttcgtgt ttataaatta catttcctgc gttatctgtg
    35881 atggaatcga tgatatagcc ttcttcatag acgccgccgt ttgctaaagc ctgaaatccg
    35941 ttggtctgtt gaagcgtcgt gacatctact gtacctaaag gagcagattc aacgccccaa
    36001 ttgtcatttg ccgggtagtt catctttgcc aagtagtgct cgtaagcata ttgtttcgat
    36061 ccaccggaat tcaacacatc ttgatataag tgataagccg ggatattgtt ggaccattct
    36121 agtgactcac ggacggtttg gaaggtgttc gatccttcgt tagtagcatt gacgattttc
    36181 tcgccagcat tttctccttc ttgccaagtt gttgcataat ctgatacacg ggattctgat
    36241 ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa ctggtttgat tgatgagcca
    36301 gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct gactgtaatt ccgtccgcca
    36361 acgaatccgt agatgcggcc agttctgtta tccatcaata catttccggt ttctacttga
    36421 ctgccagcac catcatctaa catgtatcca taattttcaa cgccattttg caatgcagca
    36481 tagacatctt tatcgattgt cgaatggatt gtatatcctt tattttggat cgtttgctgt
    36541 gccttttgat aataagcgtc actgaccgat gaatcagaca tatcagcgtt gtctttttca
    36601 gccaattgtt gtgcaatgat tcttgttgct tcattcatca cagtataata caagaattct
    36661 ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt ttgtaaggtc ataggctttt
    36721 gcttcttcgt attgttcttt cgtaatttgg ccttcacgat acatactgaa taaaacaaaa
    36781 tcttttctgg caaggccggc agaaagatct tcttttagcg cgcctgtgtt cgtgtatgga
    36841 ctatacgtga tcgggctttg gggtaatcca gcgatataag cagcttgcgg cagcgtcaca
    36901 tcttttgcat ctacaccaaa gattcctttt gcagcttcct ggactccagc aatgttttgt
    36961 cccttattat tacgaccaaa tggtgagaca ttcaaatacg tagcaatgat ctcatctttt
    37021 gaaaagtatt tttctacttg tgcagaaaga atgatctcgt ttgctttacg tttgaatgtt
    37081 gtttcatctg taaggatctg ctgtttgact aattgctgtg tcaaagttga tccaccagaa
    37141 gagccaatac cagtagcttc agatactaat gctcgtaaaa cagctttagg aacgtaacct
    37201 tggtgtttat cgaagtattc atcctctgtg ctgataattg cagttttcaa taaaggtgaa
    37261 atcttatcag atgaaatagt cgtgcgcatc agatctgtct ggatttttga tatctctgta
    37321 ttatctgcat aaacaatctt tgacgtttct gtaatgtttc ctagttctgt ctgcagtgct
    37381 tttttagtcg gcaattgtgt gtcttcgacg agataggcaa aatagccagc gcctattcca
    37441 atacctagag ctgcgaacat caaaagaaga acagtaataa aaacagtcaa tgattgaaaa
    37501 actctaaaaa taatcttagg aacaaaccat ttttcttttt ttcttctatt ctgttttttt
    37561 cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg gttttatttc gctaattata
    37621 gcaaataata gaacaaaaaa acagacaagg ctgtttttgg caggaatttc ttttttaaaa
    37681 gagttaaaac gtgagaaaat gatctttatt ataaaagagt tcttaagctt ttctttagag
    37741 aaaatttcag ttactatgga gaacagggca aagaaaaatc aatcattttc tttgtatatc
    37801 atcttaccaa tatataggaa ttgttagtaa caattgcctt tcaaggagga atccatccat
    37861 gttattaggc tcacacgtca gcatgagcgg aaagaaaatg ctgttaggag cagcagaaga
    37921 agcagccagt tatggatctt cgacttttat gatctataca ggcgcccctc aaaacactag
    37981 aagaaaagcc atcgaagaga tgaatattga tgccggaaaa gcgtacatgg cagaaaaagg
    38041 tctaacaaat atcgtcgttc atgcccctta tatcattaat ttggggaata cgatcaaacc
    38101 agaaatgttt ccttttgccg tccaatttct gagagaagag attgttcgtg cggaagcttt
    38161 aggcgctact caaattacac ttcacccagg tgctcatgta ggggcaggaa cagaagccgg
    38221 aattgctaga attattgaag gcttgaatga agttttgaca aaagaacaga cggcacaaat
    38281 cgctttggag acaatggcag gtaaaggtac ggaaatcggt cgaacatttg aggaattagc
    38341 gcaaatcatc gatggagtaa aattgaatga aaaattatcc gttacttttg acacctgtca
    38401 tacgaatgat gccggttata atattaaaga agacttttca ggtgtaatgg aagagtttga
    38461 taagatcatc gggttagatc ggcttaaagt actccatatc aatgattcta aaaatccgca
    38521 aggttcgcat aaagaccggc acgccaatat tggttttgga acaatcggat tcgacacgct
    38581 gaataagatc gtccatgatc caatgtttga caatatttcg aaaatcttgg aaactcccta
    38641 tgtaggcaaa gataaaaaga aagcttacgc accatatgga aaagaaatcg ccatgttcaa
    38701 agcgcaaaca ttcgatccag aagtgtttcc tgagttatta gcagaagaag tgatactcta
    38761 agattttcta tctgacagca gcaaaaaagg accaggcaca ttgccttgtc cttttttgtt
    38821 taagaaacaa aggagtactt tttgcagtct ttttcatcat gcatcttctc ccccctcttt
    38881 ttaaatttag acaaacaagg tatactggta gaaaagtaaa aaaacgaaaa gtttcacaag
    38941 caagtggcaa aacgtgattg gaaggacgaa aagatgctga acactttaaa acccttgatc
    39001 aaccaactaa aaaagaaaca tacatacttt attcttggca tagccctatt atttacaatt
    39061 attcgtttgt atttatatag taaagcaatc tatggaacag acatgtcggc aacctatgat
    39121 gaccagctgc tgattcgcta tgctcaaaat attttggacg gtaaatggct aggggagtat
    39181 acgactacaa cactttccaa aggaatctcc tacagcttat ttttagtact tgcacataaa
    39241 ctgcatcttt cttatgcagt tttgttcggc atgttcagta tttttgccag tatattggtt
    39301 gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc ttttaagtat ctatttttat
    39361 tttttgtttt ctccgattag ttttacacat gaatattcca ctcgaacata tcgtaatgcc
    39421 ctcgtgattc cggctgttga gatcattatc gcttgtttat tagctattta ttaccgcaaa
    39481 tacggttcta gcagaaaatt gctgccatgg tttttagggc tttctgtcgc ttttcctttc
    39541 ttttggttca ttcgcgaaga ttctatctgg cttcttcctt ttactgtagt agctttgggt
    39601 atcagtttcg tccagatctt gctagggcaa gacttttctt tcaaagaagt aaagaagtta
    39661 aaaaaacgac tctttcttct ctcgaaacgg gaatggctga aactttcatt gtttttactt
    39721 cctatagttg gcttttccat cgtctatcaa tggatcgaat ataaaaatga gacaacatac
    39781 ggtattgcaa ccatcaatga ccgttccagt ggagaatttg gacgtttgat gaaacaactg
    39841 atccgaatag atgatgaaac gaacttgaat aaagaaaaca gtgagatatg ggtctcacat
    39901 gctgcactaa ataaagcatt agccgcatct cctacattcc atactgtttc tacccgtatt
    39961 gacgaattat atacgactca cgcgtggact caaggtggaa aagtaaaaga attacccgga
    40021 gatatcatct tttgggcatt gcgagatgtg gtaagcgaat ctggttatta ccaaaacaat
    40081 gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag aattagaaga agcctatcac
    40141 aagggaacaa tcaaaaagaa aaaagagttc tatctgatgg gatcaggaga tggaaagacg
    40201 atgagtgatc tgccaatcgt attcgatttc cttcaatcag gatacgctta tgcatggcga
    40261 tataaagatt tccatcaagg aggaaattat agtttcggaa cgcaagaaca agtcaaacag
    40321 gctcaagaat tattaaacct tccgctaatg gagaattgga tcgatagtaa tgaaccgaga
    40381 acacctaatt tccaattaac aaaatcagca aaagtcgcga atgtcatcat aaaaatttac
    40441 caagttctgg ctattcccgt gctgattgct tcaatagcag ggatattact tatgatacta
    40501 ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt cctctttgat tgtggtcacc
    40561 ggattaatac tgacacaatt ggtttttctt ttcggtgtct catggttttg ttcttatgcg
    40621 ccagaaaaaa aggactattt tttaagtgtc tacactggtg caggcgtgcc attaacgcaa
    40681 ttcatcgtag taacaagcat gctagttttc agtaaattaa atctgaaaaa agctttatct
    40741 tctaaataaa aagacccttg gtagctagat ttttaaccta actatcaagg gtctttgtgt
    40801 ggaaaaatca gtttttattc gatttcttct accgtttcct gcaaaagctg ttcatatttt
    40861 ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac aagaacaatg
    40921 attacataag cgatgtgatc agccaatgct agttgagcgg aagtggcttg ctcgccgaag
    40981 aaactttcga tctgtcctgg taatcctcgt aagttcaagt aagttagact aatcacagag
    41041 aaccagccaa gaagttttac gatgaatgaa tttttaaatc ggctacccat ctccgcttgg
    41101 ctgttggtca tcatcagtaa aggaatcatc gaaaacggca acgcaaaagc caagaacacc
    41161 tgggattcat tcattaagtt attcaaagcg gcatgttctt cgatcgttcc ttttgtactt
    41221 gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac cagtcttctt
    41281 agccaaatcg ggatacgcat atggatgaat ccttccatga tgacttgacc ggtcaacgta
    41341 ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa agtggacaag
    41401 ataccagatt ttgctacagc aatcaaaata ccattgctca tagctgatgg atcagataaa
    41461 gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa gacagcgaca
    41521 cccatgatca atagcagtgc attgactaga aaagccattg acaactggat attagaatcc
    41581 caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt tttggtgatc gatcttccga
    41641 gtttgtgaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc accaataatc
    41701 cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga gaatgtatga
    41761 ctgttaggga taaagccagc aaataaactg tgccaatctg ggttagacaa gccaacttga
    41821 tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa ccagtgcttc gatcttacga
    41881 aacccgactt ttgtcagtag taagagaacg aaaacatcta atacagtaat aaagacggca
    41941 atcaccaatg gaatatcaaa aagtaagtag agggcgattg ctgcaccaat gacttctgca
    42001 atatctgttg ccataatggc taattcagtc aagatccata aaatgacacc taatgtctta
    42061 cttgtgcgtg ctcggattgc ctgtgccaaa tccatttggc tcacgattcc taattttgct
    42121 gccatatact gtaaaagcat agcaatcaga ctagacatca aaataactga catcaataaa
    42181 tattggaaat tttgtcctcc ggtgatcgaa gtagaccagt tacccgggtc catataccca
    42241 actgctacta acgcaccagg tccagagtaa gcaagaagtg ttttaaaaaa acttttcccc
    42301 tttggtacat caactgttcc atttacttca gctaaagata aaccatttgt atgttcaata
    42361 agtcgatgct tatgaaattg tttattggat tgattattca agaaaaaacc acctttccta
    42421 tttatctacg attacattct acacctcaaa aagaaaaaat taaagaaaaa gattcggtac
    42481 gcctaatttt ttttcatggc gcgtcttgct agatgacgaa agaaattttg ttctcctcta
    42541 aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt caataaactt
    42601 tagtaaaata agtcattgag agcaattcgg aaatagaaag gagagattta tatgttatca
    42661 acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt aggtggcttt
    42721 ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt taatgaggac
    42781 atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa aattcgccag
    42841 atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaacg actttttacg
    42901 ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca gattattcag
    42961 caaaaaagac aagcacagac aatcctaggc tcttgataaa gacaaaggag gagtaaaatg
    43021 tcgattttta gaaaattagg ctggtttttt cgtcaggaaa agaaacatta tttaattggg
    43081 gttttctcgc tattcgcagt ggccttggta cagttagttc ctccaaaagt gattgggatc
    43141 atcattgatg agatcgccga taaaaattta tcaataaaga tcattttcgg gtggatcttc
    43201 ctattgatta tcgctgcttt tttacaatat ctttttcgtt atatctggcg gacgaatatt
    43261 tggggaagtg cagctcgatt ggagaaagaa cttcggcagc agttattcaa acattttaca
    43321 gaaatggaca atctctttta ccaaaaatat cggactgggg atttgatggc tcacgcaacg
    43381 aatgatttga atgcgatcca gaatgtagca ggagctggta tcttgacctt tgcagattca
    43441 gtgattacgg gcggaacaac gatcattgcg atgattttgt ttattgattg gcgcttaaca
    43501 ctgattgcgc tgattcctct gcctttgctg gccgtcactt cacgagtgct ggggtcaaaa
    43561 ctccatgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga taagacacaa
    43621 gaaagtatca ctgggatcaa agtcattaaa acatttggac aagaaaaaga agatctagcg
    43681 gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa ttttttagac
    43741 tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc aacaattatt
    43801 ttaggtggaa agtatgtctt ggaaggcacg atctcgatcg ggcagatggt ctcatttatt
    43861 agttatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt caacgtattg
    43921 gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa gacgcatatc
    43981 gtggaacaac tgggtgcagt gcagaccccg gcaaaaggcg agctgaatgt agctatttcc
    44041 tcttttaatt atccgaaaga agaatcacca gcattagaac aaatcaagtt tgcgatccaa
    44101 gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac gatcatcaaa
    44161 ttactgatgc gtgaatacga tgattatcaa gggaaaatcc aatttggcgg acgttcgatc
    44221 aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca ggatcacttc
    44281 ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact ttcagaagaa
    44341 gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga attaccaaaa
    44401 ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca gaaacaaagg
    44461 atttcgattg ctcgagcatt gatcaaaaat cctgaattat taatattaga tgatgcactc
    44521 tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga aataagaaaa
    44581 gagaaaacaa ctatcattgc agctcaccgt ttaagtagcg tgatgcatgc aaaagaaatc
    44641 attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct gcttgcacta
    44701 ggcggctggt atgaacaaat gtgggagaaa cagcaattag aagcgaagat agaaggaggc
    44761 gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca cttaaagaac
    44821 agaaaaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga acgttcatcg
    44881 ttgctatctt atttgcgttt gctttgtctg tgatcaatgt cctattgcca aggatcatcc
    44941 aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt attttgtttt
    45001 ttgctggtat gtacttattt ggagtcatag taaaaagtat catttggttt ttccaatggt
    45061 atttgtattc catggcctca ttgaagacct atcagtatat tagggtaaaa ttatttgaaa
    45121 agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatca accgtttctc
    45181 gagtgacgaa tgacacagaa acgctatttg aattttggta cgtctttttg atggtattga
    45241 ctgggatctt tgcagtcgtt tcttcattcc ttgccatgtt tcaaatcaat ggagaaattg
    45301 ccttgtggac tttggtgttt ttgccgattt taggaatcgt catctggtat taccagaaat
    45361 tcagttctcg tatttaccga aatatgcggg aaaaattaag tcagctgaac acgaaattga
    45421 atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct cgtttagcta
    45481 aagaatttga acaagtgaac aatgagtatt tagacacgcg ttatgcgatg atcaaaacga
    45541 attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt gcgttggtgt
    45601 tgactttgtt tgggatcgat gcgttgcatt ctcccgtaga agttggactg atctatgcat
    45661 ttacgaccta tgtccaggga ttttttaatc caatgtctca aatgatggac tttttgagca
    45721 tcttcacaga tgggatggtt gctggtagtc gtatcttgaa gattatggat acggaagaat
    45781 atgcaccaca acaaaaccct ggagcagatc aaatgattac ttcaggtaag attgagtttc
    45841 gcaatgtaag cttttcttat gatggagagc acaatgtatt aaacaatatc tcttttgttg
    45901 ctaatccggg agagaccgtt gcattagtgg gacacaccgg aagcggaaaa agctcgatca
    45961 tcaacgtatt gatgcggttt tacgaatttt atgaaggtca gatattgatt gatgatcatg
    46021 acattcgaga atatccaatg gaagagttac gtaagaaaat ggggctggtc ttgcaagatg
    46081 ctttcttgtt ttatggggat atcgcaggaa atattcggat gatgaatcca gctatcacag
    46141 acgaacagat acgccatgcg gctgagtttg ttcaagcaaa caaattcatc gatgaattac
    46201 caaaaggata tcactcaaaa gtgatcgaac atggtgccag ctattctagt ggacaacgac
    46261 agctgatcac ttttgcgcga acaatcgtga ctgatcctaa aattctagta ttggatgaag
    46321 caacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg gcaaaaatgc
    46381 gtcaaggacg gacaacgatt gcgattgccc atcggctgtc aacgattcga gatgcagaat
    46441 tgattttagt tttagacaaa ggtcatatag tagaacgcgg gaatcatgaa acacttctag
    46501 cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtata taaaagatgt
    46561 tgtgacagta gaattttgac ctaagcacta gagtgaaaat aaaacatagc ttctcatatt
    46621 tttcatctgt gagagttagt gtcgtagtca ccagatttaa aaaaagattc gacagctagt
    46681 cagaagtgcc taatagccac taagaaagcg ttgacaaaag catttgcttt cgataatata
    46741 aagaaaacga tacaacttgg atagtgattg cctgatgcaa gctaaacctg atgagtctcc
    46801 catggtttca tggacggctt tttgggtttt tctttttgtt ttggatacag ccctatacat
    46861 catggaaagg agcaaaacat gctgattgag aaagtactga ataacaacgt cgtaataacc
    46921 agggatgaaa gaaatcagga aatgatcgtg atgggaaaag gtttggcatt caaaaagaaa
    46981 gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat ttcagttgac aaatgacggg
    47041 ctttcacaac agttccaaga attggcacaa gcgattcctc ttgagtactt agaaatttct
    47101 tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc agttgaatga ttctgtgtat
    47161 attgcattga cggatcattt gtatacggct atcgaacgtc acaaaatggg aatatttgta
    47221 ccaaatgtat tgctgtatga tatcaaacaa ctatttccta aagagtatgc catcgggaaa
    47281 aaaacagtga agaaaatctc ggataaatac cacatcaagt tatcagaaga tgaagccgga
    47341 tttatcgcat tgcatttagt caatgcacaa ggcattcaaa gccagcaaga caatacctac
    47401 ctgtttacta taacaataca ggatattcta aatatcgtcc gctattacta taagattaca
    47461 tttgatgaag attcagtcca tttttttcga ttcgtcacac acttacgttt ttttctgaca
    47521 cgcgtcctag atggagaaaa atcagaagtg ggagaagttt ctgaggaatt gtttcaggtt
    47581 attcaaaaaa aatataagga tacgtttggt tgcgttgaga aaatagcaga ctttttagcc
    47641 gagacgttca actaccagat gtccacggac gaaaaaatgt atttaacgat ccatactgcg
    47701 cgtttagtgc atgagaatcg ttgtaaataa aaaaaggagg ataaattaac aagaacgtat
    47761 gaaataaggg tagtgacatt cagttggcta aaccttccac taataaattg gagggaatag
    47821 aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga aaatgtcggc gggaaggaaa
    47881 atatcaatgg tttgacacat tgtatcacaa ggcttcgttt caaattaaaa aacgaagaaa
    47941 aagcaaatga tgagatcttg aaaaatatgg acggtattgt aacggtaatg cgagcgggag
    48001 ggcagtatca agttgtcatt gggaatcatg tgccagtcgt gtttgaagag gttataaaag
    48061 caggaaacct tacttttgat gaggctgttt caacagaaaa aatgagaccg tttgatatgc
    48121 ttattgatgg aatatcgggc tgtttccagc cattccttgc tatcctagct gctggcggga
    48181 tgattcgtgg actgacagca ttccttgtct tcttaggcgc gtttgaccgc ggttctggca
    48241 catttgttat gtttgataat atcggtgatt ctgtattcca atttatgcct gttattatcg
    48301 gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt agggatgttg cttggcgcag
    48361 cattaatgaa tcctagcttg tcgctggaag ctttgagtgg tgcagcagaa gcaccactaa
    48421 cgaccatatt ttctggcact atttttgaag cacctattta tcaaacagta ttcggcattc
    48481 cctggattgc gcgtaactac gcttcttctg tcattcccat tatctttatt gtgttgttag
    48541 ctagccaaat tcagaaacca attaaaaagt tagtacctga gatgatcgcg aatttcttcg
    48601 ttccattctt caccgttctt attacaatgc ctttaggttt tttactcgtt ggaccagtat
    48661 tcacattcgc aactgatatt ttaatggctg gatttgaaac acttttagca ctttcgccag
    48721 ttatttatgg agcaatcgta ggcttcttct ggcaaattct ggtgatgttt ggtctccatt
    48781 gggcaatcgt accaatggga ttgatgcaat tttctgtgaa tggttggcaa aatattatga
    48841 cacctgttgc agtagtgtct tttggacaaa ctgcagcact taccgcactc tactttaagc
    48901 ttagaaatcc aaaagacaaa gcaattgcaa taccagccat tgtttctggt attgtgggga
    48961 ttacagagcc agctatttat gggtttacat taccacgtaa aaaaatattt atttttacct
    49021 gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat gaatttaact tcttggaatc
    49081 aaggtggact aggcatcttt acaattccca actatattag accagatggt gatttaacag
    49141 atgtgattaa tgtattgatt gggatcgcca ttgcgatggt ggtatccttt acgctgactt
    49201 ttttcttttg gaaggatgaa gcaggtgaaa cagatgatat acagaaaaaa tcaggtaaag
    49261 aaattgtaaa aacaccgata caaggacaaa tcgctccact aaatgcagca aaggatgcag
    49321 cttttgcaca aggaacacta ggtcgaggaa tcttgatcta ccctgaaaaa ggagaagtac
    49381 gtgccccttt tgacggaaca gtcatgaccc tgttcccgac gaaacaggca attggaatgg
    49441 tttctgaaac agggctggaa ttgctgattc atgtcggatt ggatactgtg cagttggaag
    49501 ggaaatattt tgaaagctta gtgcagcaag gataaagagt gaaacaaggc gatctgttgc
    49561 tacgcttcga caaaaagaaa atcgaaaagg aaggatacag tttagaaaca cctgtgatcg
    49621 tcaccaatta cacggattat ttggatattt tggaaaacca aggggagacg gtcgggccag
    49681 acgatgcact tatcaccgca ctgacctgaa cggttaggtg aagaatggac taggcttaac
    49741 tattcttttt attccacttc tttattttca aaattgaaag gctctttcat gatggctgag
    49801 ctgatagggc gtttatcgaa tttttgaccg acagaaatta gatggactaa attctgttga
    49861 taattttctg caaaaacaca actatataac acgtctagaa gataacaaat tgatgtattg
    49921 atcgtaaaat taccaatttt tgaatacagt cgctcgcgtg tcgtaagtcg caaagcggct
    49981 gtactcattg cagatagcgt tgaatcaccg atactggtca aagctaaaat tggaacatga
    50041 ttttgtttca atatttctgc ggtacgaagg atcacattgt tttcacccgt ataagaaatc
    50101 agaatcccgc acatatcact cgtgcaattg tacgcttcat agaatgattc accaaaagtg
    50161 gtgacaatcg tgacatccat tttgatgcgc ttcattttca gcatgaaatc ttgagaaatc
    50221 aaagtattcg agttactggc gaaaatcacg attttttttg cttgaagcat tagttgtttg
    50281 gctttttgca gttggtcatg atgaatgaga gaaagtgtat catgaatcgt catctgttct
    50341 aaagaagcca ttttatttgc gatcgtcata ataccgtctg tggtggaaaa aggaagattc
    50401 gcatctacgt cttgaaaatt cccttcaaga taggctgttt cagctaaaaa agcattacga
    50461 aaactgaccc agccatcaaa acccattttt tttgctacac ggatgagtgt agaggggtgg
    50521 acataacatc gctcagcaat cgcttggatg gtcatttcgc caagtgctgg agaatcgctt
    50581 aagagaaact ggatcacttc attttctgaa ggagaaaagg agatttcttt gattttttca
    50641 cgtaataaca tgtagatacc ttcttcttat ttttttacgt aaattataac ttttttgaaa
    50701 atgatttgta aacggattta tactatataa cttaaaatag acattttttg acaagatatt
    50761 tacaaaaatc cactagaaaa cagtaaattt tgtaaatgtt actggaaaga aacagatttc
    50821 tattgtttgt tttgtttaca aaaaagataa tgaaagtgta aaaaagtaaa ggagtgagaa
    50881 catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc agcagttaca cccccgagtt
    50941 gatggaagga tttatcaaaa gatatgagga attaccgata cgagaaatct ggctcgtaga
    51001 tgtagaagaa ggaaaagaaa agcttgagat cgttagtcag atgtctcagc gtatgtggga
    51061 tgcttctccg tatgaagtca agatctatgc aactctggat cgggaagaag cattgaaaga
    51121 tgcggatttt gttacgactc agtttcgtgt cggtcaatta gatgcacgaa tcaaagacga
    51181 gcggatccct agttattatg ggatgattgg gcaagaaaca aatggtgctg gcgggatatt
    51241 caaagctttt cgtaccgttc cagtcgtttt ggcaatcgtt gaggacatga agcgtttatg
    51301 tccaaatgct tggctgatca atttcgcgaa cccaagtggg atggtgacag aagcagttat
    51361 cagatatggc aaatgggata aagtgattgg attatgcaat gtgccagtga tggcgatgat
    51421 gacagaacca gcaatgcttg gcaagaaaaa agaagagctg atctacaagt ttgccggtct
    51481 aaatcatttc cattggcaca aagtagcaga taataaagga aatgaccgca cggcagaatt
    51541 gattgatcgt ttatacgatg aagagaatgg attaccgaaa aatatctttg atatcccgtt
    51601 tttcaaagaa caattaaaac aaatgaacat gatcccttgt ggctaccacc gctattacta
    51661 tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat caaacgattg gtacaagagc
    51721 gcagcaagtg aaagaaacag aagcagagct atttgaatta tacaaagatc cagaattaga
    51781 ccataaacct gaacaattgc aaaaacgagg tggcgcttat tattcagacg ctgcctgtga
    51841 aacgattgct tcaatctatg gaaataaaga aacacagatc gtggtatcta caaagaatga
    51901 tggagctgtt cctgacctgc cggcagattg tgtagtagaa gtcactgcct atgttggcgc
    51961 acaaggagcg agaaatgtag cgtttggttc tcttcctgct gctgaaagag gctggttgca
    52021 agtaatgaag aatatggaat tgctgacgat cgaagcagct gtgactggtg attatgggac
    52081 agctttacag gcgtttacga tcaatccact tatacgatca ggaaagacag caaaacggat
    52141 catggatgag ttgtttattg cacaccagca gtatttaccg caattcaagg agacaatcag
    52201 ccgattaaaa gaagaaggaa tcgaagttca agatgaactg gcaagagaat tatcaggtaa
    52261 tacacatata atggaggaag tataatggga ttcagaaaag attttttatg gggcggtgct
    52321 acagcagcca atcaatgtga gggcggttat gatgaaggtg gacgcggact agcaaacgta
    52381 gatgttgtgc caatcggaaa agaccgtttt cctgttatca ccggaaagaa aaaaatgttt
    52441 gaatttgatg aggatcattt ttatccagca aaaattgcaa tcgatatgta tcatcgttat
    52501 aaagaagata ttgctttgtt tgcagaaatg gggttcaaga cttatcgaat gtccatcgcc
    52561 tggagtcgta tttttccaat gggggatgag aaagagccga atgaagctgg cttgaaattt
    52621 tatgaggatg cctttaaaga atgccaaaag cacggaattg agccacttgt gacgattacg
    52681 cattttgatt gtccaatgca tttgatcaaa gaatatggtg cgtggcgttc cagagaactc
    52741 gttggttttt atgaaaatct gtgtcgagtc atcttcaatc gctataaagg acttgtcaaa
    52801 tattggctga cattcaatga gatcaatatg atccttcatg caccattcat gggcgcaggg
    52861 ctgtattttg aaaaaggaga aaatgaagaa caaatcaaat atcaagcggc acatcatgag
    52921 ttggttgcaa gtgccatcgc aacgaagatt gctcatgaag tagatccgga gaacaaagta
    52981 ggctgtatgc tggcagcagg agcatattat ccatacactt gcaatcctga agatgtttgg
    53041 gcttctagaa aatcagatcg agaaaattat ttcttcattg atgtccagtc acgaggagaa
    53101 tatcccgctt atgcattgaa agaaatggag cgaaatggca ttcagatcga aatggaacca
    53161 ggggatgccg cattgctgaa agaacatact gttgatttta tctctttttc ttattatgct
    53221 tcccgagttt cgactaccga caacgaactc ttggaacaga ctgcaggaaa tatctttgct
    53281 tctgtcaaaa atccatatct gcaagcaagt gaatgggggt ggcaaattga tccattaggc
    53341 ttgagaataa cgatgaatga tatttatgat cgttatcaaa aaccattgtt tattgtagaa
    53401 aacggcttag gtgcagtcga tacgccagat gaaaatggtt atgtggaaga tgattaccga
    53461 atcgattacc tggcagcgca tatccaggcg atgaaggatg cagtagaact agacggagtc
    53521 gatctattag gctatacgac ttggggatgt attgatttag tatcagctgg taccggtgag
    53581 atgaagaaaa ggtacggatt catttatgtc gatcgtgata accaaggaaa tggcacatta
    53641 gcccgttcaa agaaaaaatc attcaactgg tataaaaaag taattgattc aaatggagaa
    53701 aatctttcga acgattgatc aatgatttat aaaagtctat tgtttttcag cggatttttc
    53761 gagtataata aagctattcg agaaacgcaa gcagaacaat agactttttt tcgcattggc
    53821 tgtaaagtat gcttacttga ttaagtaaag gagaatctac atatgtatga tgtaatcgtt
    53881 attggcgcag gtcctgctgg gatgactgct gctttatatg cttcacgttc aaacttatcc
    53941 gtattgatga tcgaacaagg tgcccctggc ggccaaatga acaatacggc agaaatcgaa
    54001 aattatcctg gattcgattc gatcatgggc cccgatctag cttataagat gtatgaaagt
    54061 gtctcaagat ttgggacaga aaatgcttat ggaatcgttc aagatatcaa ggatcacggc
    54121 agccataaag aagtcatctg tgaagataaa agttacgaag gaaaaacagt cattatcgca
    54181 acaggatgta tccaccgtaa attaggcgta cctggcgagg aagaatttgc tggacgaggc
    54241 gtttcttact gtgcagtctg tgatggtgcg tttttccgta acaaacgtct agtagtcatc
    54301 gggggtgggg actcagcagt agaagaagcc atctatttga cacaatttgc ttctgaagta
    54361 gtgatcgtcc accgtagaga tgaattacgt gcacaaaaga tcatccaaga ccgtgcattt
    54421 gcaaatgaaa aaatttcctt tatgtgggat acagtcgttg acgaaatcgt tggaaacgaa
    54481 atggtagtga caggtgtgaa agcacgtaat gtcaaaaccg gcaatgaatt tgatttagca
    54541 gcagacggag tattcatcta tgttggactg gatccattaa ctgcaccttt tagagaagca
    54601 ggtttgacga acgaagctgg ctggattcca acagacaacg aaatgaaaac gaatatgcca
    54661 ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc gacaaatcac tacagctgtt
    54721 ggtgacggcg gtattgctgg acaacaagtg tataaatact tggagtcttt agaagaaaca
    54781 acagcataag tttaaaaaga gtagaagaac gggttcaatt gttcttttac tctttttttt
    54841 cgcgtgaaat ttactgaatc tgatttatgc gaacacgtcc aacacctttt gataaaagaa
    54901 tcctttcttg atttaagcgt tttttggtcc tcatactttc atatctaata agtgaatgct
    54961 atacttatta agaaccatat ttcagaaaga ggtgttattt tcatgtcttg ggaacaagtg
    55021 tatgaacaat gggtacatga agaaaattta gaagaaaact taaaaaaaca attaaatgat
    55081 ctgggtgaag atcctgaaaa acttgaagat gctttttatg caccgttgga attcggtact
    55141 gcaggtatgc ggggaatctt aggacctggg atcaatcgaa tgaacatcta tacggtaaga
    55201 caagcaacag aaggactagc acgtttcatg gatacacaag gtgaagaaac aaaacgccga
    55261 ggtgtcgcta tcgcttatga ctcacgccat cagtcaccag aatttgcaat ggaagcagcc
    55321 aaaacactag cacatcacaa tatcccgtct tatgtattcg aaagtctgcg tccaacacca
    55381 gagctttctt ttgcagttcg tcatttgaag acgtttgcgg gaatcatggt aaccgcatct
    55441 cacaacccag cggcttacaa tggatataaa gtctacggag aagatggcgg acaaatgcct
    55501 cctgcagacg cagatgcatt gacgaaatat gtccgtgaag tcagcaatcc gctaaaagtc
    55561 gaagtattat ctgacgaaga agctaaacat agcgggttga tcacgatcat cggagaagaa
    55621 gtggacgctg cttatctaga agaaatcaaa gcagtgacga ttgatcgtga attggttgaa
    55681 acaatgggga aagatttgaa acttgtgtac acgccacttc atggaacagg gaaaatgttg
    55741 ggcgaacgtg cgttgaaaca agctggattc gaacaattcg tactagttcc cgaacaagct
    55801 gtggcagatc cagatttcac tacggtcaaa tcgccaaatc cagaagaaca ttcagctttt
    55861 gaatatgcta tacgacttgg agaaaaagaa ggagctgact tgctgattgc aacagatcct
    55921 gatgcagacc gtttaggcgc agcggttcgt ttgccagatg gttcttatga agtattgact
    55981 gggaaccaaa tcggagcatt gtttgctcaa tatattttgg aagcacatca atcaagaggc
    56041 actttaccag aaaacgcagc ggtattgaaa tcaatcgttt ccagcgaatt gcctacagca
    56101 attgcggaac actacggtgc taccatgttc aatgtgttga cagggtttaa attcattgct
    56161 gaaaaaatcc aacaatttga agaagaccat tcacatactt ttatgtttgg attcgaagaa
    56221 agttatggtt atttagttaa accttttgtt cgcgataaag acgcgatcca agcattgctt
    56281 ttattagcag aagtagcagc ttactataaa aaacaaggca aaacgttgta tgacggcttg
    56341 caaacgattt ttgaaaccta tggctatttc gcagaaaaaa cgatttctgt gacaatgagc
    56401 ggacaagaag gttctgcgaa aatctctgct ttgatggaaa aattccgtaa agaagcaccg
    56461 acagaatttg ccggagcaaa agttattcaa acagaagact ttaaagaatt gacgagaaca
    56521 gaccgtgaag gaaacgtaga aaaactaaca acaccgccat ccgacgtatt gaagtataca
    56581 ttggaagaaa atggctggat cgctatccgc ccttcaggaa ctgagccgaa aatcaagttt
    56641 tatattggtg taaaaggaag ctctacagaa gacgcagccg aaaaagtagc ctcttatgaa
    56701 gcgattatca aaaagatgac agaagaataa aaaacttgag actgatggaa aaagtggtaa
    56761 actttagaag tcagtctttt ttcaagttgg aggtgaatgg attgccagac acgcaaaaat
    56821 caaaagtaga gaaagtcagt caattattca aaatgttgag tgatcccact cgtcttaaaa
    56881 ttctgctata tctgaaagac ggggaacaaa atgtgacagc aatcacccaa gcagtggaaa
    56941 tggagcagtc tgctgtatcg catcaactac gtttattaag agaaaatcat gtcgtcaagt
    57001 ctcatcgcga gggaaaagcg attttgtata gtcttgatga ttctcatgta ctggatattt
    57061 tgaaccagac actcaaacat gtggaacatc aataacgata ggtgaaaata gataaacact
    57121 ggagtgtgac aacgaagaaa tttcgttgtc acactccagt gttttttaag aaaaagacaa
    57181 aaatgattaa tcgctttttt ctgttgtttc agttccataa taactatgat cttctaggtc
    57241 taaagcattc aaaataagcg aagccgtttc ttcaatagat aaagatgcca cattgatcac
    57301 tacacatcct agtttttcat aaagatcatt cgcaaaatcg agttcagctc taattttttc
    57361 gatatcggaa taagcagtat cagggtttaa tccgtaagag cgcatccgtt ctttacggat
    57421 attatttaaa atatctgggt tattcgttag tcccacgatt tttttaggat cgatttccca
    57481 cagctgtttg ggaatatgtg cttggggaat caaaggtaaa ttagcaactt ttaaattttt
    57541 atttgctaaa aacaagctca aaggcgtttt ggaagttcgg gatacgccta gcaaaacgac
    57601 atctgcttct aaaaaaccac gaggatcttt cccatcatcg tattttacag caaattccat
    57661 tgctttgatc cgtttgaaat agttctcatt caataaatgg agggcaccag gttgccgaat
    57721 tggcttgata ccagttaaac gttcaatttc tgatatcggt ggcgtcatca catcgaaact
    57781 aaataattga tgttcttcac aaaaagaacg tgttatctcg atcagttctt gattgattaa
    57841 tgtatgcagg acaagtgctt tttcttctaa tgcatcttgc aatgcctgcc ttaatgtttt
    57901 ttcatctgtc acgaacgttc tgcggaatag gttgaactca acggtaggat actgagccat
    57961 agtagcttgc gctagttttg tcgcagtttc tccagcagag tcggagatga caaaaaaagt
    58021 aacgatttct gtagaatcgg aagacatgga taaaacctca cttgcatttt ttttatgttt
    58081 attataccat actctagaat gcgtacttcg taatcatttt gatttggagg aataagtcat
    58141 gaaaaaacag acattgatcg aacaatcatt agaaacattg aaaaataacg gttttaaata
    58201 tactaaaaaa agagaagcat tgttaactta tttagtgaag cgtaaccgct acgtatctgc
    58261 taaagaagtt tttgactata tgaatcagga attcaaaggg gtaagttatg atacgattta
    58321 ccggaatctc catgattttg cagagatcga tttactggaa gaaaccgatt taaatggtga
    58381 aatgaagttt cgttttcatt gctgtcaggg ggaaatcggc catcaccatc attttatctg
    58441 tacagtgtgc ggaacaacga aagaaatcca tatgtgtcca atgaattatt tcgaagaaca
    58501 gctaggtgga tgcacgatcg aagggcatag attcgagatt ttcggaagat gtgaaaattg
    58561 tcaataagga tttaaataaa ataacatttg ataatttaaa aaaaaagcaa gatttccttg
    58621 acggtcaaaa cagcattaga tataatattt acaggacagt cttgtttttt gagatatatt
    58681 tcaataacaa ttacggttac tttttaagct cggagggagg gaattaacat gtcaaaaaca
    58741 gtcgttcgca aaaacgaatc acttgacgat gctcttcgtc gcttcaaacg ttccgtgtca
    58801 aaagcgggta ctttacaaga atctcgtaaa cgtgaattct acgaaaaacc aagtgtaaga
    58861 cgcaagaaaa aatctgaagc agctagaaaa cgcaaaaaat tctagttctg tgatgaattg
    58921 gagttggtag tgtgtcacta ctaagtagat taaacgatga catgaaagca gcgatgaaag
    58981 cgaaagataa ggaatcttta caagtcatcc gaatgatcaa gtcatctata caaaacgagc
    59041 agattaaaga aggtcacgac ttgacagaag aagaagaact aacggtgttg tctcgcgaaa
    59101 tgaaacagcg acgtgattct ttgcatgagt ttgaagaagc aggacgtgac gatttagctg
    59161 aaaaagtaaa aagtgaaatt gtaatagtcg aaaaatatat gccggaacaa ctttccgacg
    59221 aagaaattcg acaacttgtt caagaagcaa tcactcaaac tggtgcttca tcaatgaaag
    59281 aatttggcaa agttatgggt gcaattatgc ctaaagtcaa aggaaaagca gatggaaacc
    59341 aagtgaatgc gattgtgaaa gaactattac aatcttaatt ttatcattta aactgcggac
    59401 agtgattgtc cgcagttttt tgtgttcgta aaaaaagaac taaaaaacag ctagaagaaa
    59461 gaataagatt ctttcttcta gctgtttttt tcctgtttaa tatctcttat gttaaattgt
    59521 gtttgatttg gttattcttc tggtagttca tttgcttctt gtttataccc tgataggcga
    59581 taggcgtaat aaaagagtaa gctgccagcg taaccaagta acgttgggac catccagcca
    59641 agtcctaatg cagcaaaagg aacgtgatga tgataaaatc ctagtaagct tgctacgaca
    59701 gtttgctgac tcactaatgc aggcgaattt gccaacatat ccaatacggc tggaacggca
    59761 gaaaagaaca tcgttacttg atagacaaat ggactctttt ggaagaattt tgctgttaag
    59821 gaaagcaaga ttaacgcaag tgacaatgga tacaaaagca tcaatactgg taaagaccat
    59881 tggatgatat tatccaatcc ggcattcgca acgacaaaag agacaaaagt cgttaaccgc
    59941 agccatgtca tataactgac tttagggaac agtttatgga aatcttgtgc aaaagaaaca
    60001 acgagtccca tagcggtagt gaagacacct aatgtgacca ttacccctaa gaagctagca
    60061 ccgaagttac ctaaataaaa attagtgatc tgagaaagtg cagttccgcc attttctgat
    60121 aaaggcatct tattcaaact catcgcaccg agaaggacca gaccaaaata aatcatgact
    60181 tctaacgcaa cgctcaggct accagctttt gctgttaatt ctgtgacttt tttatcttta
    60241 aaaccaagat cacgaactgc atggacaaaa gtgacactta aagcaagtaa agctactgca
    60301 tcaaccgtgt tgtatccttc taaaacgcca tttgtcaaag cgttcgtctg ataagaatct
    60361 gcagcagatt ggtttagatt ccccatgggt tggaataaag aaacgacaaa aatgatagct
    60421 aataatgcaa gaaacaagga gtttaaatac tttccgacat atttgaccaa acgtgttgga
    60481 tgtacagtaa ggaaataagc aagtaaaaag aaaagagcag agaataaaaa catacctaat
    60541 gaatcatatt tttcaggcaa aaagggttta gcagccattt caaaagcagt tgctgctgtc
    60601 cgcggtgtac caaagaatgg tccgatcgtt aaatgaacaa ggatcaagaa caaggcagca
    60661 taccattttc cgacaggttt agctaagtca tacagaccat cactttttgt cactactaca
    60721 gctaagatag ctaataatgg gaaaagagcg cctgagatgg caaatcctac accagcgctg
    60781 atccagtttg atccagccat ttgacctaag tggacaggaa agatcagatt acctgacccg
    60841 aagaacatcc cgaagataag tgaactgacc atcaataatt gcttccatga ataagttttt
    60901 gtttcttcca taaatttttc ctccaataat tatcattttg agtaacaaaa taaacgttgc
    60961 agaaactaga aatgccacta atatcagatt ccatgaaaac caccacctta aaatataaaa
    61021 aaaagccctt ttccacttgg gaaaagggct ctagattcta gaacggtacc accttttttc
    61081 gcttaaaaaa gcttcttcat tcacggacgc ttttcgataa agttatccgc tagcctgata
    61141 atggaggcag ccacaatgat ctacgttttc gacattgact cggaaatgat gttcgtctat
    61201 tcgatttatt gtcatttctc acttacatga ctcactggga ataaatcgct gaatagccta
    61261 ctgttttcct cgacgctttg ctgtatattt atttgttgac gttataatac acctgttttt
    61321 tttgaaatgc aagaatattt caaatatttt gttttctttt tttgaaacaa gaaaaatacc
    61381 cagagaatac ttgctaagtt agacggaata cttgtcctct atcttatgca tagcttacat
    61441 gatcagcttt ttttattcaa acgagacctt tagagaatgg aagcagctat tttttaacgt
    61501 ttcttggtac ttatggttaa gcaactcttt tagaaccttt tcttatttgc tatatagttt
    61561 cttctgagta tggtaaaata ggaaagaatg aatcgaaaaa aaggggatgt ctatttttga
    61621 cggaagagtc tagttcttta gagattagat tgacggctgc tgatgatgtc agcatgcttt
    61681 taggtgcaca cgacaagcat ataaaactga ttgaagaaac aacagaaaca accatccata
    61741 cacgtggaga aatcattcag atcattggcg aacaatccca gatttccttg gctgcctcag
    61801 tcattcggac attgcaggaa ttgatcaaac gaggaatcca tgtaagtaca ccagatgtcg
    61861 tgacggcact gaaaatgggg caaaaaggca cattagatta ttttgttgaa atgtatgagg
    61921 aagagatcgt caaagaccgt aatggaaaac caatacgggt aaaaaacagc ggccaaaaga
    61981 aatatgtcga atcggtagcc aaacatgata tcgtatttgg cgtaggacca gccgggacag
    62041 gaaaaacttt tcttgcagta gtattagcaa tcgctgcctt gaaaaaaggt gaagtgcaaa
    62101 aaatcatttt gacgcggcct gctgtggaag caggggaaaa tctaggcttt cttcccgggg
    62161 atctaaaaga aaaagtagat ccttatctgc gtcctgttta tgatgcttta taccaaattt
    62221 ttggattaga tcatacgaac cgattgatgg aacgaggcgt gatcgaaatt gctcctttag
    62281 cttacatgcg ggggcggaca ttggatgatg cattcgttat tttggatgaa gcacagaaca
    62341 cgacagttgc tcagatgaaa atgttcctga cacgtcttgg ttatcaatca aaaatgattg
    62401 tcaatggaga tacgagtcag attgatctgc ctcgagggac aacaagtggt cttgttcatg
    62461 cagaacgaac attaaagcaa atcaagaaaa tcgattttgt taattttgaa gcaagtgacg
    62521 ttgttcggca ccctgtagtt gccgaaatca tcaaagctta tgaaaaggca gatctccatc
    62581 aagaataagc tttgcttagg aggataagca catgttgaat aaaacactgc agaaactaca
    62641 agaaaaactt ggaaaggcag cattgccttt cctgctgttc ttgtttttta tcattatctg
    62701 cggcgttaca tttagtagtg tcaaacaaaa ggccaatgat ttcagagaag gccaagtagc
    62761 agaagaaagt atccgtgcga acaaaacgat cgagaacacg gaagaaaccg aacaaaaaag
    62821 aaaactggca gcagaagcag tcactccaga atacacttac caaaaagatc ttgcagatga
    62881 ccagaacaat cggatcaagc aactatttga attgatcgat aaaacgaatg atgccatcaa
    62941 caaatcttat gacgaaaaga ttgataaggc aaaggaagat gaaaatatcc caaagcctac
    63001 tacagaagag cggatcgccg cgttgaagaa aaattttgaa aatgtgaatg cagaaaatgt
    63061 tgcgttttat caaaaactac cgaacaattt ttattcgacg atatttgaaa tgactgaaac
    63121 tgagatccat actgtgaggg atgagagtct gaagctcatt gacgaacaga tgagcaaaca
    63181 agttcgtgaa agtgaattgg aagcttttaa acaagaagca gaagagcaaa tccaatattt
    63241 gaatgtcaca ccagcacaac aacaaatgat tcgctactta gtggatcaag gtatcgtagt
    63301 gaatgacgta ttgaatgaga aaaagacaga agaattgaaa caatccgcac gagaagctgt
    63361 acaaccagtg atgatcttcc aaggagaaat cattgttcgt gaaggtaatc aaatcgatgc
    63421 ttcagctatg aaaaaattgg aattactagg actgactaat caaacaactt cgatttttcc
    63481 gctaattgcc atggttttag ctgttttgct tcaaatcggc gtactgattt ataattcact
    63541 tcaattccaa aatgcaggag aaagaacaaa atacgtgctt ttctatgtga cagctatgtc
    63601 agtcagcata gtgctgatga aatttttcca attattccaa acggaacaag ctacttatat
    63661 cccgctgttt tatcccgctg cttttgcacc acttgtttta agctttttct tgaaccgaag
    63721 agcagggatc atggctggta ttttccaagc agtatctgca cttttcattt tttatgattc
    63781 gattggcacg aactttctaa cgattatctt gatgtcttat gttttctctg ggctcatggc
    63841 aacgattgtc agacgcaaac ggatctcaga acaaggatta tctgctgcca tgtgggtcat
    63901 tatttttcct gtgatgatgg atgtcatttt gatcgtctat caaggaatga gctttggtga
    63961 tgcaacgact tggttaatgc ttgtctgcgg gttcactggt gcactcttct cattcttgct
    64021 gacgatcggt ctacacccat atattgaatt gatggtcaat gatgacagtg taattgtatt
    64081 aaatgaactc agcaatccaa atcatccttt attgaaacag ttgctggaag aagcgccagg
    64141 tacttatcat catagtatga tggtagctaa tctaagtgct aacgctgtag cagaaatcgg
    64201 cggacgttct ttgctaactc gagtagcttg ctattatcat gatatcggca agatcaaaca
    64261 tgctagcttt ttcgttgaga acttgccttc aggagcggaa aacccgcaca atttcttatt
    64321 gccagaagac agtaaacata ttatctttgg acatgtgaca gatggagcca aaatattgga
    64381 agattataat atgccgcaaa tggtgatcga tatttgcaaa cagcatcatg gaaccacgct
    64441 aatgcgttat ttctacatca aagcgaaaga acgcaatccg gaagtaacag aagagcaatt
    64501 ccgttatcct ggaccaaaac cgcaaagtcg tgaagcaggg atcgtcaata ttgctgacag
    64561 ttgtgaggct gcagtacggg caatggatca tccgacgagc gaaaaaatcg aaagttttgt
    64621 ccataatctt atcgaagagc gtatttccga tggtcaatta gacgatagtg gactgacctt
    64681 gaaagaaatc cgaaaagtgg aaaaatcatt gatcagcggt ttaagtagca ccttccattc
    64741 tcggatcaaa tatcctaaaa tgaaaacaga agttgagaag ataaaggaag aacaagaaga
    64801 atagaaagaa gcgatgtata atggatatta cctttatgga tgaaacaaac caagtctcag
    64861 aagagaaaat gaaagaaatc gatgacttgc tgcaatttgc agcatcttat ttaaagctgc
    64921 cagaggaaac agaaatgtct gttactttca tggacaatgc ggcaatccaa gtaatcaatc
    64981 gtgattaccg tggaaaagat gcaccgacag acgtcatcag ttttgctctg gaagaagaag
    65041 gcgaagacga aatcccagtg atttttgaag atggagagaa tccattgcct cgtgtactag
    65101 gagacatcat gatttctatt gaacgtgctg ctgaacaagc agaagcttac ggccattcat
    65161 atgatcggga acttggcttt ttagcggtac atggattctt gcatatcaac gggtatgacc
    65221 atatgacacc agaagatgag aaagaaatgt ttggtttaca gaaagagatc ttggatgcct
    65281 atggactcaa aagataaaaa cgtagagaaa aacaaacatt tcctgatgtc tgtggaattt
    65341 gctgtccaag gagtcaaaac cgtttttgag gaagaacgca atatgaggaa acatgttgcg
    65401 ttcggtatcc ttgctttatt tatgggcgtg attttccagc ttgatcggat agagtggtta
    65461 tggctgcttt tagctgtttt tctcgtttgg atagtagaga tcatcaatac tgtttttgaa
    65521 aatgtagtgg acatgtttac ggattttcat tttcatccga ttgggaaaaa aatcaaagat
    65581 atggcagctg gagcagtttt agtgaccgct tgttttgctg tcatcattgg gttgatttta
    65641 tttgtcccta aaatatatca tttgttgttt aattaaaaag gagaacgtat atgccagaac
    65701 ataaatcagg gtttgtcgca atcgtcggcc gcccgaatgt aggaaaatcc acattattga
    65761 accgaatcgt gggtcaaaaa attgccatta tgagtgacaa ggcacagaca actagaaata
    65821 aaatccaagg agtatatact acaccagaag ctcagctgat ttttatcgat acaccaggaa
    65881 tccataaacc aaagcatcgt ctaggagatt ttatggtaga agcagcttac agtgcgttga
    65941 aagaagtgga tgcagtctta tttatgatca gtgctgatca aaaacgcggc cggggagacg
    66001 attttattat tgaacgtcta aaaaacgtgc attctccagt ttatctgatt atcaataaaa
    66061 tcgataagat ccatccagac gagttgctgg gaatcatcga agattattcg acgcagatgc
    66121 cttttgcaca agtgattccg atctctgcta cagaaggaaa caatgtcgaa cgcttaatgg
    66181 atgtattagt cagtgaaatg ccagagggcc cacaatattt tccagatgat caagtgactg
    66241 atcatcctga atactttatc gtttctgaat tgatccgtga gaaagtcttg tttttaacac
    66301 gagacgaagt gcctcattct gtcgcagtcg ttgttgattc gatgaagcga aacgagaatg
    66361 ataaaatcca tatccaggcc acgatcatcg tcgaaagaga tagccaaaaa ggaatcatta
    66421 tcggcaaagg cggaaaaatg ctgaagcaag taggaacgaa agcaagaaag gatatcgaaa
    66481 atcttttagg agacaaagta tttctagaac tttgggtcaa agtccaaaaa gactggcgag
    66541 acaaaaaagt ctatctgcaa gactttggct accgaaaaga cgaatactga tattctgaac
    66601 ttcttatagg ttaagtatag aaataaactg gataaaaatc cgcacgttga gaaaaacggc
    66661 ggaagatcga aacataaata atctggatcg gcagaatctg cttttccaga tttatttttt
    66721 aggagggaga atatgagcca gcttgcagaa tcgaaaggct tgattttatc tagccgtaat
    66781 tacaaagaaa aagataaatt agtcaaaatc tttacggaat cttctggcaa aatgatgttt
    66841 tatatcaaag gaatccatcg gcaaaatcag ccattagctc cggctttgct cccctttacc
    66901 gaagcagtct atatcggtaa ttttcgaagt gaaggtttgt cgtttttaag cagtgtcaaa
    66961 gacgtccatg cgtttcggaa catccaagaa gatatctttt tgagcggtta tggtacctat
    67021 ttgctaaatc tagtagatgc agcaatcgaa gatcatcaat atgacccgca tttattccaa
    67081 tttacgcaac aagctctgca gcggatggat caaggagatg atgcagaaat catcacgaat
    67141 atctttgaag ttcaaatact gcaacgcttc ggaatcgcgc caatttggac gcactgtgtc
    67201 gtatgcgggg aaacaaaagg aaaatttgac tattcttcca aatctggcgg tgtgatatgc
    67261 gagaaacact ggccaatgga cgaacaccgt tatcatgcag atcctcgcgc tgtttatttc
    67321 gttcgaatgt tttcggctat ttcttatgat aagatcagtg gaatccactt gaaagaagaa
    67381 acgaagcagg caatccgtga cctcattgac cagttgtatg atgaatatgt cggtattcat
    67441 ctaaaaagca aaaagttcat tgatcaaatg aagtcttggg cacacgtatt aaagacgcca
    67501 gagaaaaata tgcctgaaaa agagatacct gaaaaagaag aataaatgaa agatacaaca
    67561 aaaaaatcaa aactatttga caaatacagg gacttccgtc tattattaac cgtagattaa
    67621 atagatcgtt cactgagagg atctattata taaacaagaa aactgcaaaa tgaatacgta
    67681 cattgaccaa gatcagtaaa aaacacgtgg tttagcgagt tcgggagagt ggaagccgga
    67741 cccaaacgat ttttgaagag gcacttgcga gtacgataaa tgaagctgcc gaatctttat
    67801 tcggaagtag ggtggaaccg cgaaattatt cgtccctatg tctgtaatca gacataggga
    67861 ctttttgtcg ttttctttcc tgtattctta ttgacagcaa cgcttaaaaa ttgaaagggg
    67921 caaaacagat gagtaaaaaa ttgaccgtcc aagatatgat tttaacatta caaaaatttt
    67981 ggtcatcaaa cggctgcatg ctgatgcaag cttatgacac agaaaaagga gcaggtacga
    68041 tgagcccata tacattctta cgggcaatcg gaccggaacc atggaatgct gcttatgtag
    68101 agccatcccg ccgaccagct gacgggcgtt atggagaaaa cccaaaccgt ttgtatcagc
    68161 atcatcaatt tcaagtcgta atgaaacctt cacctgaaaa tatccaagag ctttatttag
    68221 aaagcttgcg tcttttagga attgatccat tagaacacga tattcgtttt gttgaagaca
    68281 actgggagaa cccatcgatg gggtgtgccg gtgtgggttg ggaagtatgg cttgatggga
    68341 tggagatcac acaattcacg tacttccagc aagtcggagg actaccttgt aaaccagtta
    68401 cttctgagat cacttatgga ctagaacgtc ttgcttcata tattcaagaa gttgaaagtg
    68461 tctatgatct ggaatgggct gatggtgtga aatatggcga gatcttcaaa caaccagaat
    68521 atgaacattc caaatattct tttgaagtaa gtgaccaaga cttgctacta agcaactttg
    68581 accgttttga aaaagaagct aaacgctgta tcgatgaaaa tcttgttcat ccagcgtatg
    68641 attatatttt gaaatgcagc catacattca atttattaga tgctcgcggt gcagtatccg
    68701 taacagaacg agcaggttat ctttcccgta tccgcaacat ggctcgtgct gtagccaaaa
    68761 tctttgtggc agaacgcgaa aaattaggat tccctttatt gaataaaaac acagcaaatc
    68821 cagaaaacat gaaggaggct gaataagatg gcaaaagact tattacttga aatcgggctg
    68881 gaagaaatgc ctgctcatgt tgtcacacca agcatgaaac aattagaaga aaaaacagca
    68941 aaatttttag atgaaaacca attgacttat gatacgatcg aaacgttttc tacaccgcgc
    69001 cgcttagcaa tcaaagtaac aaacattcca gaacgtcaag cagacagtga agaagaagtc
    69061 aaaggaccag ccaaaaaaat tgctttggac gcagatggaa actggacgaa agcagctgaa
    69121 ggatttgtcc gcggacaagg tctgacgaca gatgctatca cttttagaga attaaaaggc
    69181 gtggaatatg tctatgtaac gaaatacact cacggcaaag aagcgaaaga agtattgaca
    69241 ggtttagcag atgtcattaa aagcctcact ttcccagtaa cgatgcattg ggcagaatac
    69301 gattttgaat atatccgccc tattcactgg atcgtggcat tgttagatga cgaagtaatc
    69361 ccgtttgaga tcttagatgt caaaacaggc agagcctctc gaggacatcg tttcttaggc
    69421 gaagatgtga catttgcgca tgcaaaagaa tatgaagaaa aattgaatga acaattcgtt
    69481 attgcttcac cgcaaaaaag acaagaaatg atcgttacac aagcaaatga attagccgaa
    69541 aaaaatcact gggttattga tctagacgaa gatttactag aagaagtcaa caatttagtc
    69601 gaatatccaa ctgtcttttc tggcgacttc gaagaaaaat atctttctgt tcctgaagaa
    69661 gtactagtca cttctatgaa agaacatcaa cgttatttcg aggtacggaa cgatcaaggt
    69721 atgctgcttc ctcattttat ttctgctcgt aatggtgatc gggtaaaatt ggaaaatgtg
    69781 gttcgaggca atcaaaaagt attgacggct cgtttagaag atgcggaatt cttttataat
    69841 gaagacaaga aagcttcaat cgaccaatac gttgaaaaac taaaaactgt tactttccat
    69901 gaaaaaatcg gtagtatcta tgagaaaatg cagcgggtac aagtcattgc ccgtatcatt
    69961 ggcgaacaag taggactatc tgaggaagaa ttgatggatc tgcaacgagc tgctgaaatc
    70021 tataaatttg atctagtaac caacatggta ggggaatttc ctgaactaca agggatcatg
    70081 ggcgaaaaat atgctctgct gaaaggcgag aaacctgctg tagcaacagc gattcgtgag
    70141 cattacatgc ctatatccag tgaaggagag ctacctcagt ctacagtagg ttctgtttta
    70201 gcgattgcag ataaattaga tagcattttt tctttctttg cagttgggat gattccaagc
    70261 gggtcaaacg atccttatgc cttacgtcgc caagcatacg gtgtcgttcg gatcatcgaa
    70321 tcgaaaaaat gggcctttcc tttgtctgtt ctgcaagaaa cgattagtga tgttatcagt
    70381 aaagatacgg atcgtttcgg cattggttta tcagaagggc aacaacaggt gatcgacttt
    70441 atcaaaggcc gtctgcgcca attattgaca acgaagaata ttcgtcatga tgtcatcgaa
    70501 gctgttttga atgcagaaca aaaagatctg acaaaagtat ttgcagcagc acaattgttc
    70561 aagcagcatc ttgcagatga agacttcaaa ccatcgatgg aagctttgac acgtgtcgtg
    70621 aacttagcga aaaaagcgga gcttgaaaaa caatcagaag tagaccctga actgttcgaa
    70681 aatgaagcgg agaaagaact gcacaaagca gtagaggcaa tgaaaaaaag ctttgctgac
    70741 caaacgatca acgaaaatta tgatcagctt gtaagccttc gcccactgat cgaaaattat
    70801 tttgatcaga cgatggtcat ggcagaagaa ccagcagtac gtaagaaccg attaacgcaa
    70861 ttaagtcaaa ttgctgccat ggctttgtcg ctagcaagtt tagatcaaat catcacaaaa
    70921 taatttctaa tatgtaaata aaccagagaa taggcacaaa at
//
LOCUS       DACDTO010000007        60556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDTO010000007 DACDTO010000000
VERSION     DACDTO010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 60556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.007"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWK70_000483"
     CDS             591..1280
                     /locus_tag="IWK70_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9613056.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWK70_000484"
     CDS             1267..2475
                     /locus_tag="IWK70_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9613057.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWK70_000485"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWK70_000485"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9613058.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWK70_000486"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWK70_000486"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9613059.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWK70_000487"
     CDS             complement(6578..7273)
                     /locus_tag="IWK70_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9613060.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWK70_000488"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWK70_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9613061.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWK70_000489"
     CDS             complement(8472..8663)
                     /locus_tag="IWK70_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9613062.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWK70_000490"
     CDS             complement(8684..9574)
                     /locus_tag="IWK70_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9613063.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWK70_000491"
     CDS             complement(9561..10328)
                     /locus_tag="IWK70_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9613064.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWK70_000492"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWK70_000492"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9613065.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWK70_000493"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWK70_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9613066.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWK70_000494"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWK70_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9613067.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWK70_000495"
     CDS             14944..15282
                     /locus_tag="IWK70_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613068.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWK70_000496"
     CDS             complement(15450..16208)
                     /locus_tag="IWK70_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9613069.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWK70_000497"
     CDS             complement(16226..17044)
                     /locus_tag="IWK70_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9613070.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWK70_000498"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWK70_000498"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9613071.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWK70_000499"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWK70_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9613072.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWK70_000500"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWK70_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9613073.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWK70_000501"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWK70_000501"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9613074.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWK70_000502"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWK70_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9613075.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWK70_000503"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWK70_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9613076.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWK70_000504"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWK70_000504"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9613077.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWK70_000505"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWK70_000505"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9613078.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWK70_000506"
     CDS             27086..27385
                     /locus_tag="IWK70_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9613079.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWK70_000507"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWK70_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9613080.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWK70_000508"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWK70_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9613081.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="IWK70_000509"
     CDS             28352..30325
                     /locus_tag="IWK70_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP9613082.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="IWK70_000510"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="IWK70_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9613083.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="IWK70_000511"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="IWK70_000511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9613084.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="IWK70_000512"
     CDS             33204..34496
                     /locus_tag="IWK70_000512"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9613085.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35232..36095
                     /locus_tag="IWK70_000513"
     CDS             35232..36095
                     /locus_tag="IWK70_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9613086.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36224..37846)
                     /locus_tag="IWK70_000514"
     CDS             complement(36224..37846)
                     /locus_tag="IWK70_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9613087.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38095..38616
                     /locus_tag="IWK70_000515"
     CDS             38095..38616
                     /locus_tag="IWK70_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613088.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IWK70_000516"
     CDS             complement(38802..40532)
                     /gene="spxB"
                     /locus_tag="IWK70_000516"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9613089.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40697..41515
                     /locus_tag="IWK70_000517"
     CDS             40697..41515
                     /locus_tag="IWK70_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9613090.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41618..41815)
                     /locus_tag="IWK70_000518"
     CDS             complement(41618..41815)
                     /locus_tag="IWK70_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613091.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41984..42562
                     /locus_tag="IWK70_000519"
     CDS             41984..42562
                     /locus_tag="IWK70_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9613092.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(43607..44110)
                     /locus_tag="IWK70_000520"
     CDS             complement(43607..44110)
                     /locus_tag="IWK70_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9613093.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44469..45494
                     /locus_tag="IWK70_000521"
     CDS             44469..45494
                     /locus_tag="IWK70_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9613094.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45817..46695
                     /locus_tag="IWK70_000522"
     CDS             45817..46695
                     /locus_tag="IWK70_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613095.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47171..47287
                     /locus_tag="IWK70_000523"
     CDS             47171..47287
                     /locus_tag="IWK70_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9613096.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48215..48553
                     /locus_tag="IWK70_000524"
     CDS             48215..48553
                     /locus_tag="IWK70_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613097.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48647..48877
                     /locus_tag="IWK70_000525"
     CDS             48647..48877
                     /locus_tag="IWK70_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613098.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49277..49648
                     /locus_tag="IWK70_000526"
     CDS             49277..49648
                     /locus_tag="IWK70_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9613099.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49922..50614)
                     /locus_tag="IWK70_000527"
     CDS             complement(49922..50614)
                     /locus_tag="IWK70_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9613100.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50617..51402)
                     /locus_tag="IWK70_000528"
     CDS             complement(50617..51402)
                     /locus_tag="IWK70_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9613101.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51639..53039
                     /gene="lacG"
                     /locus_tag="IWK70_000529"
     CDS             51639..53039
                     /gene="lacG"
                     /locus_tag="IWK70_000529"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9613102.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53064..53381
                     /locus_tag="IWK70_000530"
     CDS             53064..53381
                     /locus_tag="IWK70_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9613103.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53394..55067
                     /locus_tag="IWK70_000531"
     CDS             53394..55067
                     /locus_tag="IWK70_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9613104.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55081..56712
                     /locus_tag="IWK70_000532"
     CDS             55081..56712
                     /locus_tag="IWK70_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9613105.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57584..58774
                     /locus_tag="IWK70_000533"
     CDS             57584..58774
                     /locus_tag="IWK70_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9613106.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58908..>60556
                     /locus_tag="IWK70_000534"
     CDS             58908..>60556
                     /locus_tag="IWK70_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9613107.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLF"
BASE COUNT        18746 a        11099 c        12012 g        18699 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaacaaa cggtgttcaa aagcttgcat ctacggcact aagcccaatc
    34681 agatgaaaaa tatggagtgc tatttttatc tgattgttct tactgcttga tgcagagcag
    34741 ctttttcaca acctctccac taaacggtgt tctcctgcca actcctcggt cacaagtcac
    34801 aatattcaac aaacatgaga agctgttttt tgaaattgtt ccttgtgact cggagctaaa
    34861 cggcaggctc acaacctctt ggtaaacggt gttcaatcag ctgaccctcg gtattagctc
    34921 aaaaaacggc aaaatatgag gaactatttt ttccgttttt ttctctaata ctcaggtcaa
    34981 aacgctgatt tcacaacctc tgattcacgg tgttccagaa gtagcttcta cggaaataag
    35041 ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat ttcttgaagc
    35101 tgaccacttc tgtcacaacc tcttattttg attagaactt gcgaaaataa accggttaaa
    35161 gtgtgtctag cgcaagagat ttgctataat agacgataag ggtaaaatgt aacgagaagg
    35221 ggttgaaaaa tgtgtatcaa attgtaacag attcttgttg tgatctgcca taccatgtat
    35281 tagcaaaaga aaaagttgat tttttatcaa tgaatctcca attgaatgat caagagttag
    35341 tagatgacct cggaaagaca ttcaattatg accgctacat gaatcagctg aaaaatggtg
    35401 cgatgcctac gacatcacaa atcaatgttg gccgatattt agaatttttc cgttcttatg
    35461 cggaaaaaca gattcctgtt ttatatgtgg ctttttccag tggattaagc ggctcttaca
    35521 gtagtgcgct tcaagcggtt gaattgctga aggaagaata tgaagaccct gagatctatg
    35581 tttttgatac aaaagctgca agtattgggg aagggctttt agtattggaa gctgtaagat
    35641 tgaaaaaaga aggtaaaagc atcgaagaaa ccctagagtg gctggaaagt aattatttga
    35701 aagtccattc atgggtaaca gtagacgatc tgaaatatct agaacgaggc ggtaggattt
    35761 ccaaagcaag tgctactctt ggcagtctat tgagtgtaaa gcctattatt acggtggata
    35821 aagaaggaag gctgacgaat gttgacaaag ttcgaggaag aaacaagtcg atccagacaa
    35881 tcgtcaatga gacggtaaaa acaatcgttg caccacttga tcaaatcgtt tatgtggctt
    35941 acgctgggga tctagagagc gcggagaagg caaaacggct tttggaagag cagataaaga
    36001 tgaaagatgt gaagctttat cccttaggac caacgatcgc cagtcacaca ggctatggct
    36061 gtatcgcgat tttttcgatg ggaatcagtc gataaaagca gtaactcatt ctgtaaaaaa
    36121 gcccagaaat tgattgtaac aaaaacagcg actcttaaaa gatagatgag catcctatct
    36181 cttagaggtc gctgtttttt tattttttaa gctaaatagg ccattatgcc cataactcgt
    36241 tcaattgttt ttgaatatct gcattttcca aaaattcatc atatgtcgta tcagctcgat
    36301 cgatcacacc tttatccgaa acggcaatga tgcggtttgc caatgtttgg ataaattgat
    36361 ggtcatgtga cgcaaataag attgagccag taaatgccat caagccgtca ttcaatgctg
    36421 tgattgattc taagtctaaa tggttcgttg gatcatctaa tacaagtacg ttagctttcg
    36481 aaagcatcat ttttgaaagc atgcaacgga ctttctctcc accagataaa acattcactg
    36541 gtttcaatac ttcttcacca gaaaacaaca ttcttcctaa gaagcttctt aagaaggtgt
    36601 tatcatcttc ttctttactt gcatattgac gcaaccagtc taaaatcgtt agatcagtat
    36661 cgaattcttt cgttgtatcc tttggtaaat aagaattact tgttgtcaca ccccaacgta
    36721 ctgttccagt atctggcgta atttcaccca tgataacttt gaatagcgtt gttgttgtta
    36781 tatcattttc tgcgacgaaa gcgaccttgt cttctttgct caaattaaaa gatatatcat
    36841 ctaaaatttt ctttccatca atcgttacac ttacattttc tacctgcaat aagtcgtttc
    36901 caatctcacg ttcaggtttg aatccaacga atggataacg tctagaagaa ggttggatat
    36961 cgtctaaagt aattttttca agcatttttt tacgtgaagt tgcttgtttt gattttgatg
    37021 cattcgcact aaagcgggca ataaattctt ggagttcttt gatttgttct tcttttttgg
    37081 catttgcgtt tgcttgaagt ttagccgcta gttgactaga ttccaaccaa aaatcataat
    37141 ttcctacata aagtttgatt ttaccaaaat ctagatccgc catatgtgta catactttgt
    37201 tcaaaaagtg acggtcatgg gatacaacga tcacagtatt ttcaaaattg atcaaaaatt
    37261 cttccagcca attgatcgat tggatatcca gaccgtttgt tggttcgtct agcaaaagaa
    37321 catcaggttt accaaatagt gtttgtgcta ataacacttt taccttttgt ccaccagtta
    37381 attcactcat ttgctgatgg tgcaattctt cagggatgtt cagaccttgc agtaaaacag
    37441 ctgcttctgg ttcagcttcc catccattaa gctcagcaaa ttctccttct aattcagctg
    37501 ctcgaatacc atcttcgtca gtgaaatcct ctttcatgta aatcgcatct ttttctttca
    37561 gcacttcata cagacgcttg tgtcccatga tgactgtttc aagtacagta tagtcttcat
    37621 agtcgtaatg gttctgtttc aatacggcca tccgttcatc tggtcccata gtaacggaac
    37681 ctgtggaagg ctgcagatcc cctgataaca ctttaaggaa cgttgattta cctgcaccat
    37741 ttgcaccaat caagccatag cagtttccgg gggtaaattt aatattcaca tcatcaaata
    37801 gtttgcggtc tgaaaagtgc aaactcacat catttacagt aatcaatttt tttcctcact
    37861 tttatcatta ttagttttga caagtcttat agaattatat aggggaaagg taaaagattc
    37921 aaggcaatcg aacattcttt gtgtttcacg tgaaacagaa tgaaaaaaat aaaaataaaa
    37981 gcggagcgaa aatagctact ataggattta ttagtttgca acagcataat ttttgacgtt
    38041 atttataaga gggagtacta taaaactgta aaagaaatga aagaaggatt gaaaatggat
    38101 gaaaaaagaa ccatcgactg gggctcattg attttgggaa ttttatttgt acttgtctca
    38161 ttgttatcat ttcaagaccc tgtcggaaat ttagttgcaa ttgtagtagt ctttgcgatt
    38221 tttgccttta ttaaagggat atttgaactg tttgtacgga acagattaaa agagctaaca
    38281 ggatataaag gaaagatgcc cttaataatc gggatcatcg atattctagt tggcgtcttt
    38341 ttcttattca atattggtgc aggagtcgca gcattgcctt ttgtttttgc agtatggttt
    38401 attgctgatt cagttttagc gttatttact gctgacttgg caagaggagt aagtgaagga
    38461 tattattggt ttacgatcat cgtcaatatt ttaggaattt tattaggaat tttcttattg
    38521 tttaatccaa tttcttctgc gttgacatta agctttttag ttggattcta ttttatgtta
    38581 tttggtatca cacatatcgt ctatgcattt agataagtaa aatcaaacaa ataaaaaagc
    38641 cggcttccga ttacagtttt actgttgtaa ttggaagccg gcttttttat catggacaaa
    38701 tatgcacgag gtgattttta ggtgtcgaga caaatttatg caaaaatgtg agaagctata
    38761 tacacaaatt ttatcttaat actcgaagca tagcgcagtt gtcacaacct cttttcatat
    38821 aattcattca atactggcaa atcatggact tcatattttt ctttgaatgc ttggatttct
    38881 tcttcagaat attttcttgg atctaaatgc agatcttcta ctggcaatgg gcgcttgtta
    38941 ttgatcttca catcgattac tacaggacga tctgattttg cagctgcatc aaaggcaaat
    39001 cgaagttgcg agtattctgt gactgtaaag ctatttgccc cgagcgcttc tccaacttta
    39061 ccgaaatctg catcttctaa aaatacacca aatttctttt gttccgtatc ttcttgttct
    39121 gcttcgatga aaccaaaaga atcattcgaa aagacaacat taataattgg aagctgatat
    39181 ttgacttgtg taatgatatc ttgcatgacc atagcaaacc cgccatcacc acttaaggta
    39241 aatacttgtt tttctgggta gcttagttgt gcggcaattc ctcctggcac accattaccc
    39301 atagttgcga accatccaga tgtcgtatgt ttttgtttac cgttcatatt caataaacga
    39361 atagaatgga tagtcgtgtt accgacatct gtgacgaaaa tcgcatcgtc ttcagcaata
    39421 cgattgattt ctttatagac agcctctgga cgaatcggtt cttcctctcg atcttcaaaa
    39481 gatttcaacc aatttaccca tttttcttta ttttcttgat ttgctaggta ccagctatca
    39541 gcagggcgag catcccctaa ctcgacaagt ctttgcaaag ctgtgtttgc atcaccgagg
    39601 atcgacaaag atgtttcatg gcgtcgtcca aattttgacg catcgatatc gatttggata
    39661 aattcagcag ttggattaaa aaatgcacgt ccaaatggga agtcgctacc gacaaataag
    39721 atcagatcgg cttctgccaa tgcttcattt ccgggtttag tagcgacacg tccagcaaaa
    39781 ccaagaaagt tttcataatc atcaggaata attccttttg ctagaacgga agcgacaaca
    39841 ggaactgaaa aatgttctga gaattctttg atttgcggaa atccgtttcg cgttccttga
    39901 ccaacataaa gaactggttt ttttgctttt tcaagataag gcaaagcagc tagcaaatct
    39961 ttttcttctg gtaatatcac acttgttttg tgagtgtgag cggtggagaa aggttgttcg
    40021 tcaatttctt caaaaccaag atcaacaggg atagtcacga tagccacgcc tttatgctca
    40081 taggcggctt taatggcttc atctactaca tgcggaagac tttgtggtgt catgactgta
    40141 cgattgtata cgctaacatc tgcgaaaata ggattttcat ttagttcctg gaaagagtta
    40201 tagttcatgg atgtagaagc aacttgtcct aagaacgcta ctagaggcac atggtccatc
    40261 tgtgcatcat acaaaccatt gatcaaatgt gtcgcaccag gtcctgcaga gccgaagaca
    40321 gcgccgactt tccccgttaa ttttgcatct gcagcggctg cgagtgcgcc gacttcttca
    40381 tgacgaactt gaatgtagtt gatttcggtt ttttcttgat atagcgcatc cataattgaa
    40441 ttgaaagatc ctccgggaat gccgtaaata tggtcgatac cccagctttc aagaactttt
    40501 accattgcga ttcctgcatt gatttttgac atggtgtttc actcctttag caatgatgat
    40561 acataaattt tttgtatcta gagtatagaa ctttttatcg aaaatcccaa ctaattcgtc
    40621 ttttcacaaa aatgtagtca tgtttttggg aatatgctag agtagtacat agatagattc
    40681 aagagagggg aatggcatgg ttgaatggct attgagactt gaaccttggc agcaagcact
    40741 tgctgggaca gcttttactt attttatgac agctcttggt gccgggttgg tttttttctt
    40801 taaagaaatc aaaaaagaag tgctaaactt gatgttagga tttgcttctg gtgtcatgat
    40861 tgctgcaagt ttttggtctt tgttagatcc ggctattaca aaagcagaag aaaatggaga
    40921 tattgcttgg ctagttgtaa gtattgggtt tggtttaggc ggtgtgtttt tatatatggc
    40981 agataaaaca ctgccccata tgcattttgg accacaacat gaagcagaag gattgcctac
    41041 ccatttaaaa cggacgatct tgcttgtttt ttctattaca ttgcataaca ttcctgaagg
    41101 tttagccgtg ggggtagcat tcggtgcagc agcaactgcg gataatccga cagccgctat
    41161 attagccgct gtttcggtgg cgctaggtat tggtatccaa aacttcccag aaggagcagc
    41221 tgtatcaatc ccgttgcgac aagaaggtct gagtcgtaaa aaggcctttg tttacggaca
    41281 agcttctgga atcgttgaac caattgctgg agtgattgga gcgctactag taacgaaagt
    41341 ggagctgctg cttccttatg cacttgcttt tgccgcagga gcaatgattt atgtcgtggt
    41401 ggaagaattg atccctgaag cacagcagac attgtccagc aaacgtcatt ttgcagtttt
    41461 tggcgtcatg agtggattta tcattatgat gattttggat gtggcattag gataaataaa
    41521 aaaaatatat atgatatatc aaatagggcc gcgaacttat atcgcgaccc tattttttta
    41581 gtaagttata tcataatgaa gtgaatggta tcatacatta ataatagttt tttctatgtg
    41641 tatttccttt gatccagcga acaagccaga tgataagtgc gattgggatt agaactttga
    41701 ccgcaaacca taaaagcccg cttaggacac tgacaaaaag actgatgacg aagctagctg
    41761 caattagcac taaaagaacc atgaagatat ttaagaacat atttttttga cgcatacaaa
    41821 accctcctct tgttcattct ttactataga aaaagtataa cagggattgt ttaaaaacac
    41881 ctccaactga agactgattt ttacttcatc tacttaaatg atcaaacaat tgctttttct
    41941 ctggtttatc atctaaaata gagcggaaaa ggaggagaat atcatgccta aaaatcgtct
    42001 tgaagcattt acagatgctg taatcgcgat tatcatgact attttagttt tagaacttca
    42061 tgaacctaac ggagacacgt gggaggcgct cgctgcatta ggttaccgat tttttatcta
    42121 tattgtcagt tttatcattt tagcgattta ttggaacaat catcatcatt tatttcaagt
    42181 tgtacataag ataaatggac gggtcttatg ggcgaacagt ttctttgttt ttgcgctttc
    42241 gctttttccg tttgcaactt catggatcag tgagtacatc aatagcctgg ctccagaaat
    42301 gacttatggt tttgttacct tgaccgctaa tattgcgtat tatatcttgg caaaagaact
    42361 tgtcaaagca aataaaggcc acgagaaagc attgaaggcg atgttgaata attacaagaa
    42421 atcgtatcta tcgattggtt tgaatgtgtt aggaattgtc ttagggtatt tagtagcgcc
    42481 agtagccgtg ctgatcgcaa acatcgtgat tctagctgct tggatcatcc caagtcgtac
    42541 catcgaatca caatatcggt aaaatgatcc gtgtttaaaa tttaagagaa aaatatagaa
    42601 aaaataaata aacgatcaaa tatccgagtg atactagtat tcaaagaggg atgcagatgg
    42661 atcaaaaaag aattcatgta ggactgatta ctagttttgt cacgatgctt atttatttgg
    42721 atcaaggtca tttgacttgg acagtagttg gtgtcgtagg atttttgaca ttattcacag
    42781 caataaaaaa ataaacatct ttatgtaatc tgatgttcta tctatttaag aaaagcatgt
    42841 ttacagcagt atgattctgt tgtaaacgtg tttttatttg tggaaaattt aatttttaga
    42901 aaagtgtatc gaaaaataga tagttagttt ttccacagtg gataacttcg taagagataa
    42961 aaataaaaaa ttagtcaaag aagggcgtat agatttcttt tgataggaga tatacaacag
    43021 aaatgaataa atattaacgt gttttcaaac agtataaaac ggtgtggttt ttggaataaa
    43081 aaaataacat atctcatttt acgcttttgt tattttatac ttaaaaaata acgacacaga
    43141 aaataccgtg tctcagattt ctctactact tctaaaaact actgtttttt tcctttgaaa
    43201 tgccttcaaa aagtaggatc aggcagaata tactggcaga atcaaggaga agggagccta
    43261 aataaggaga aaaaccatag aaaatataaa gtaattactt ataaaacaag ctttttatag
    43321 aaaaataata tacctgagta gggtataaat taagacggaa ttcctttcta gattgagata
    43381 ttgactagga agaatgttaa gattttaagc ctttaagcta aaaaaaaacc ctttttgtta
    43441 aataagggga atatagcctg tcagtttcaa actgcaagtc gtcctgctgc aataatatag
    43501 tagattagag atgaaaatag atttttttaa accaatgact gaataaaaag accgtcatag
    43561 atacacattg gaatgtgtct attacggtct ttttttacga acgattttat tgaagacgga
    43621 cagttcgggt caaaaataaa aaagctacga tcagaaataa gaataaaatg tatcccaaaa
    43681 taatggctag acggtctaac ttatttgtcg atagatgttt gtttagaagt tgataggccc
    43741 ataaacctat cagtcctcct gttgtattag ttatcaaatc agtgatgtcc gctacgccaa
    43801 taccgaatag gaattgaatc aattctgcca agaagcttac aaggaaaata atccctaatt
    43861 ttttagaaaa agaaagtctc ttgaaattga cattcaaaag caccccaaaa ggaaaaaaga
    43921 caatcaagtt ataagtggtc tcacgtaata ccgtcgtatt ttcaaaagaa aaagtagaaa
    43981 aagggacgag attgacattg ctgtaagaat aagtgagaaa ttgtggaaga ttaaatgata
    44041 gcttgaataa aatcaaccat aatagaaaga ataaataaat ccctaataaa aattttgata
    44101 cataccgcat agacatctcc tccatttaaa aagtatacca tcgaaaaccg ctgaaaaatc
    44161 ataaaaaagt atgaagttta aggaccaacg tagttatcgt acgaaaatta gaagcttttt
    44221 tttgttggga atccggtttc tttaacaaag ctaagatagc gagttgcttg gaaaagttgc
    44281 tatctttttt ctgtcaaaac gactcggaat gttcgttttt tgttctaatt tctccaagtt
    44341 gtttactttt tgatactaag tttatgaaaa atcaagaaat agttgttata ctaggtaatg
    44401 ctgacaaacg ggattggcat ttcatcttgg ttcagctttt tttccaatta ctaggaagga
    44461 gggatagatt gttcaagact tattttcgtt tcaataaaat acaagataat ccttttcgta
    44521 tcatgaatct gatgctactt atctttattg tattatggtt aggttacata aatcatgatt
    44581 ttttgatttc aagtttttca tcattaggga tctttacttt tttctattat caaaacatac
    44641 cgatgaaata tttgctgaaa cgtatgtttc tgatcggttg cggactgttg gttgctttcg
    44701 ttttggggat cttatcgact tatgttttat ggcttgaacc atttgccgta gctttggttg
    44761 ctttttccag cagattcgtg ttgcgattat tccatatttc taaaccaggt ggcttgtttt
    44821 ttgcgatgct ttctgccatg ggaacgagca tgcagctgcc aatcgctcaa ttaccgatcg
    44881 tctcacttta tttttttatg ggtgtcgttt ttgccctgat tgcggcagtg attacaaaat
    44941 tactggattc acggcctgag cagacaattg aaaaagcaac tttaaaagaa agatttcacg
    45001 aagagccttt agtgatcatt gattctgttt tctacagcgc tgccttattt ctttccgttt
    45061 atgtcagtca cggcttgaat ctgcataatc cttattggtt gaccttgtca tgtgcatcga
    45121 ttttgctagc agaaaacttg gatgcgatga agcatcgaca agtccaatat ctgattggat
    45181 cgatgggtgg gttatgtgtg tcggcattct tgtcatttgt gccgtttacg cagttgcaga
    45241 ccatttttct gatcactttt ctatatggaa tcgcacaatt tctagttgct agaaattatg
    45301 cagttgctaa tatttttctt aatccgatgg cgttgatgct atctacactt attcgtggag
    45361 catacttgat ttctttgatc gaatatcgtt ttttagggat cgtcatagga agtttcatcg
    45421 gtttaggtgt tgcgtgggta atgacggttg gtttgcaaca ttatttaacg gttgttcgtc
    45481 aaaaattaga atagaaggac aaatatatgt gccaaaagag gttatgacaa gatttttgtc
    45541 acaacctcta attcaaggtg ttcaaaagcc agattctcag cattaagtca aattcacaca
    45601 aaaatgtgga aaggaacttt caaaaaaatt ttatatttat tctatcaaaa aattttgttt
    45661 atgatggtaa ctgaataaac tttcctgaga tggtcaatgc tttccttgaa agtcttacag
    45721 atattctttc ttcatagaaa gtttaagttt tatagttagt gtacaataaa agtatgttta
    45781 taccatatgg tgtccggata atcagtcatt taaacaatga ggttcaaaga tatgaaaagg
    45841 ggaaatttga tgaagaaaat aagcaatgaa atccaaattc tggcagaaac tttaactctt
    45901 cccaaagtcg ttatagagca aatgattgta caagcaaaaa acatccttga tgaggtgttt
    45961 gagcaatata gtcatgaact aatggatcct aataaagcaa aggcgactgc aaagaaaata
    46021 gacaaagtct atgaagagga accttttgca gcattagcta tttatctata tgcagcttct
    46081 caaagttgga aaaaaattta tcaaccattg aagattccaa gaactatcta tttggcaaca
    46141 atgaatgctt ttactagatt tattcatgaa catttccaag taacaggaag ctatcgcttt
    46201 gaccgtggtt tttggatttg gcattacact agtggcttga tctttcggat tggtgaattg
    46261 gagtacgaaa tgacatattt tccgaaaggg tacacggatc taagattaga aggaaagcag
    46321 tgcctttctc ttcatattcc ttccgatgca gatttatctc agaataagat aagagatagt
    46381 tacgaaagtg ccattcgttt ttttgaactt tattttcctg attaccatta ccaagtgatg
    46441 tacacagata cttggttact atcacccaat cttactaaat ggttgaaaaa agagtcaaaa
    46501 atctgtcttt ttgcagccga ttatcgatta ttatcagttg atgaacagga cgatagtgga
    46561 gtcccttgga tctttggacg agtagatgcc caaatacatg attatccaga aagtacgagt
    46621 ttacagcgac aagcaaaaga gcaattgctt gcaggcgagc acattggctc tggcttaggt
    46681 attcttactt cataagttta gtacttcgaa atagtttaaa agagcgtgag acaaaagtaa
    46741 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcagaa gcaactcctt
    46801 cggaaataag ctgaaattca caaaaatttg aaaagcaatt ttcgtgaatt tcttcttatt
    46861 tcttggagtt aaacgcttct gtcccagcct cttttttagt gttctatcga acacacagta
    46921 cgttcaatag gtaaactagc tataacaata gatattaaag ttaacggaca agagggcctt
    46981 ttttaggtag ctaaaacgta ataatgaaaa tttatttcca gagataggag tcaatcagct
    47041 agtctgatcg actgttttaa caagttgact ttcattttaa cttttgatac catttaaaac
    47101 acaaatcgta attgtttcga tttgtgtttt ttagcatgac tacaatcaaa gaagaaagtg
    47161 ggtcgtccat atggaaaaaa cagatatctc aagtgcatac cgccgcttga aaagcccaaa
    47221 tatcaaaaca cgaaaacgtg cgctgaagat catcaaagat gtgaaacgga atagtgggaa
    47281 aaggtaagaa gaaggaaact taattaaaaa aatagccgaa aaacgttttt cggctatttt
    47341 caataatcgg tttttgtgta aggtttccga aagaactgct gttgattttc catttttaac
    47401 atatgataaa ttggtagaat ctttcttttc aaattgatac cattccctaa ttccagttat
    47461 atagttaact acagaaccac aaagcacgta cgtttataaa agtattatct gtagttattt
    47521 ttcaattccc tttcaacctc ttattttgac cagtagtatt gatcacttta ccttttaaca
    47581 cctttgttcg tttttgaaac atttttagtc aagaatggaa acgtaactac ataatcaaag
    47641 gttttgcgaa atatttcatg aaatatttca tgtagaagcg agcaatacgg ttgcaaataa
    47701 ccattatcgt ttaataagca gttacgaaag ctcccaatat ctctaacgtt cagctcactc
    47761 catttctttt cttcaaaaaa atcagtgatt cgtatctctt tctatttgtc gaaaaagtta
    47821 actttaaaaa gtaacgccga aaaaatatgc aaaaaaataa aaaagcagtt actattgcta
    47881 gaataacatc aaggaataat aagttttggt tttcagataa tagtcgataa gttgtgtatt
    47941 ggaattttag atacagtaat aaagtatttt tcatgaaatt ttgtgagaat cactattaca
    48001 agtatcttta ataaaattaa tggttaagga ggaacttaaa gtagaatgta aatgaatagt
    48061 tgtagtcagt cgattgtgat actagactca aagttgcagg aataagtttt tcaatttgtg
    48121 attggaagat aaaaaatgaa aaagaagggc ttttttttat actttgttga ttgtagtgtt
    48181 tattttccct ctgtttagcc aagtaacatt ttctatgact caaccagttt caagtgagga
    48241 acatgataac ctttttcgtg ctacagaaga gtttaagaaa tatcaggaaa atattgttcc
    48301 ggagactcca ttagctagtg agccaattac tgatattgat ggaaaccaaa taggatggta
    48361 tatacagtac gaagttatgg tggtaaataa tgactcaata gtaaaaaata cagtaggctt
    48421 agagctaaca ccgattgaaa aggtgagttc attgttaacg tatatttata ctgatgaaag
    48481 tgacgagact gagatcattt taacggatta ctcaaaaatt gctactgata aaaagtatat
    48541 attgtggatg tagacactag tgaaatactt tatgatatag aaattgatga aaatttacta
    48601 gatgtagcac aagaaatcga tcatcaaaag caggaagcta ttcgatatgc tggtagaaaa
    48661 gggaaaagaa aatatttatt acgtgaatgt agcaaaagtt agagaagatg aaaatgagtg
    48721 gaaagaattc aagagtcgct actcaattaa ctcgactccg acatttacag tttacagaga
    48781 aggatcgata gaaaaaactg ttttttggac taaagaaagt ggaatgtctt tagctgaagt
    48841 agaagagttt ttggattatg ttagtatgca acagtaaggg agttcttatt atgaaaaatt
    48901 ttgacacata ttatgttatt gttttttcga tatctttgct gttaatatta ctaactttaa
    48961 tgcagtataa agagtatcca gagtatttta ttttaacttt tgtatattta gtaagtagct
    49021 acacacttct atataaattc agaagttctt ctagagtttc taaagtttgt ttcttaatta
    49081 gcttattgat tctataatat ttttgattta tataggaaat taaaaatatt atagaatcaa
    49141 cactcagaga acgggttggt ttttaataga tttaataagt actgataatg aaatttctcc
    49201 tggttgggaa aacgaagttg gttaccctgt agaagaattt tttcaagatt actttgcgga
    49261 gtatgccgat aggataatga gagattacgt aacagataaa actcaagaag tcatcaatca
    49321 atcccagtca ttaaatgaat tatctgataa ggcgaaagaa gaagcagttg ccattatttc
    49381 ttcagggatt agtgactata ttgatatgag tggtgaaatc aattatggcg ataaagacaa
    49441 aattgtacgc atgataaaag cttttatcaa aacttttgat gtgaaggcag atcttaagtt
    49501 taatgatact tacctagtag gaaaattaat taatatcctt gggacagaga aagatacgga
    49561 aacgtttatt tcctctttgt ctatgttgtt tacagataag catttttcaa ctcagcttgg
    49621 tggagaggaa ctagtggaaa tcttttaacc attacgccca gcattaataa aagtatatca
    49681 aaaaagtact cctgatgatt tttaagattg gtttgagaaa taatgtgaaa acactatttt
    49741 taatgttaat tatagataga gatgaacgcg tcatatggtt aaaagaatga aaaagtcaaa
    49801 gatacactgc tatatttcac taaggatata gcagtgtatc tttcatgtcg tttcactggc
    49861 agttttttat aaaaggaggt gtgcatttca gttgcttttc tttggtagtt agttgaatcg
    49921 tttaagaatt aacggatgtg gcagacaggt gctctgtcca aaaaccagta aaatctttga
    49981 tggttccatc tttatttagc tgtgcctgtt caaatactgt tttgttttta ccgaatggat
    50041 caattgcaat aattgttccg atccccgcac gtttggcagc aagtaatcct gagtaagcat
    50101 cttcgataac aagacaatca gccggagcta agctcagttt gtctgccgct ttaagaaaaa
    50161 tatccggttg cggtttccca gggaaagagc cgtcgtcata gacaatcttg tcccagtcaa
    50221 accagcgggc taaatcgaaa atatcaaaat aaaaagcaac attctctttg acagtagccg
    50281 tggcaatcgt taatggaatc tgttggtcag tcagctggtc aagagtcgct tcaagccctt
    50341 ttgtcaactg aagctcgttt tcattttgca agcaaagcat acgataatag ctttcttttt
    50401 cttctgacaa tacggcgatc tcagaggcgg tcaaatgctc tgaaatgaaa tgtttcaaaa
    50461 tctccgtatt cgtccgacca tgaatatttt ttaaaatatc ctcgtctgat aaaccatctt
    50521 ttgtatgatc atgtatcata tgcagccaag cagcttcatg cagatgagag tccaaaaaca
    50581 ttgttccgtt gaaatcaaat ataatccctt tcatagttac gctcctatag tggtattttc
    50641 ggttgaatca gcaatttgca gatcgacata ttgtgcatag tgctcttgaa cctcccgcga
    50701 gatctggcga tccgtaatga gcagatcgaa atcatgcaaa ttataaaaca cataaaaatc
    50761 atctcgattg aattttttat tatcaacgag tagatatttc ttacgggaat tatttagagc
    50821 gatctgctgt gcttctcctt cttcggtact gtaagtagag atcgcttcgt tgtgaacgcc
    50881 atttgaactg ataaatgctt ttgtaaactt tagtcttctt aaattgtcgt tggtgatcgg
    50941 tccgacaaat gcgccggtat ttttccggaa atcaccgcca atcagtagaa tttctggtcc
    51001 atctgcttga gcaagaatat caaaaactgg ataattattt gtaatgatcc gaattttctt
    51061 gttcagtaaa tgcgtcgcca atagttcgat tgttgtacct ggtcctaaga aaatcgtttc
    51121 tccctcgtga atcaaggagg ctgctgtggc agcaatttct tttttttctt gaatctgcag
    51181 agtggctttt tcagtgtgag aaagttcttg atctaaagtg taggaaagac tttgagcccc
    51241 tccatggaca cgcagcagct tgcccgattt ttctaattca tctaaatctc tgcgaacggt
    51301 catatcagaa acatctaatt gattgatgat ttcattaacg gtaaggattc cttttttatt
    51361 gaccatttct acgatagtaa gcaatctttc ttttttcaac attgtcttca cctctaattt
    51421 aaggataatc tatcatataa acggacaaca gtcaatctaa ttcgaacaaa agtaaacatt
    51481 tagggtggtt tttatgtttg ttacaataga ttttattgct gttttctggt ttttatgatt
    51541 atttgtgttt gtttttgttg acaaatgttt tggatgcgtt tacaatgcaa tcagttagaa
    51601 agagcgaaaa ctgattatag atttgaggag agatgtacat gcttaaatta ccagaagatt
    51661 ttatttttgg cggagccaca gcagcctatc aggttgaagg agccactaag gaaggtggca
    51721 aaggcgctgt tgcttgggat gactttttag aggaacaagg gcgattcagt cccgatccgg
    51781 caagtgattt ttatcatcaa tatgcaaagg acattgaatt gtgtgaacgt tttggtgtaa
    51841 atggtctaag attatccatt gcttggagcc gcatttttcc tgatggagct gggaaaccaa
    51901 atccagaagg aatcgctttt tatcatcgtg tgttcgaaga atgtaagaaa agaaacgtaa
    51961 cgccgtttgt gacgttgcat cattttgata cccctaaacg tttgtttgac catggagatt
    52021 ttttgaatcg agaaacaata gaagcatttg tttcctatgc cattttttgt tttcatgaat
    52081 tcaaagaagt caaggtgtgg agtaccttta atgaaatcta tccagttgca accaatcaat
    52141 atttgttagg tgttttccca ccaggaatca aatatgattt tacaaagatc gtcgcctgtc
    52201 ttcataatat gatggttgct catgcccgtg tagtgaacta tttcaaagaa aacgaacttc
    52261 cgggtgaaat tggtgtagtt cactcattag agacgaaata tgcggcaaca gacgcgccgg
    52321 aagataagca tgctgccttt ttagacgatg cattgtcgat ccgtttctta ttagatgcta
    52381 cctatttagg ctattattca actgaaacct tgactgctct tgatgagata tgtgaagcaa
    52441 atcaggccag ctatcatttt cctgaagaag atttcgttga attgaaaaaa gcatccacgc
    52501 gtaatgacta cttagggatc aatcactacc aatgccattt tgtcaaagct tacgatggag
    52561 aaaatgcaat ccatcataac ggtacgggtg aaaaagggac ctctgtttat aaagtgaagg
    52621 gaatcggaga acgtatttat aaagaaggaa ttcctcgaac tgattgggac tggctgattt
    52681 atccagaagg tctatatgac ttgttgctgc gaattaaaag cgattaccca cattacaaca
    52741 aaatctatat cactgagaac gggatggggt ataaagatca gtttgaagat ggcattatta
    52801 tggatcagcc gagaatcgac tatttgagag tctatttgga atccttaagc aaggcgataa
    52861 cagccggggt aaatgtgaag gggtattttt tgtggtcgct tatggatctt ttctcatgga
    52921 caaatgggta taacaaaagg tatggcttgt tttatgttga ttttgaaacc cagaagcgtt
    52981 acccaaaaga atcagcttac tggtacaagt tggtcagtga aaccaaaacg attatttaat
    53041 aagtgataat ggaggaagaa aagatgaata aagaggaatt acaaatgctt ggttttgaga
    53101 tcgttgcata ttcaggcgac gctcgcagta cattattgaa attattgaaa gaagtaagac
    53161 aaggaaactt tgaacatgtt gattcggcgt tgaaagaagc cgacgaaaat ttaacccttg
    53221 cacataattc gcaaacaaaa attttagcgg aagaagcatc tgggaaagag atggagatgg
    53281 gattcatttt tatccatggg caggatcatt taatgaccac tttattgctg agagatctga
    53341 ttcaagactt tattgtactg tacagacaga atcaaggata ggagaagaac gctatggatg
    53401 gaatcattaa acaaatcgaa aaagggaaac cattttttga aaaaatctca cgtaatagtt
    53461 atttaggagc cgttcgtgac ggatttttaa cagcgatgcc agctatttta ttttcaagca
    53521 ttttcatttt gattgctgcg gttcctgaga tttttggtta ccaatggcct gaatctgtct
    53581 ccacatggct atggaaagtc tataattatt cgatgggtgt tgtagggtta ctcgtttcag
    53641 caaccactgc tcgctgcttg gctgattcga tgaatcgtcg cttaccgaaa aacaaagtaa
    53701 tcaacaccac ctctgtgatg ctggcctctg tggtaagttt cctgttgtta agcgtctctc
    53761 cgattgaagg tggattttca acggaatata tggggacaaa gggactactg gcttcctttg
    53821 tcgctgcatt tttaacagtt aatttatata agttttgtgt gctgaaagat attaccatta
    53881 aaatgcctaa agaagttcct ggaacgattt cgcaaacctt ccgagatatt tttcctttct
    53941 ctttttcagt atttgcggcc gttttaattg atttaggcat tcgacaaatc ttccaaatct
    54001 catttgcaga agcaatcatt acggtgctgc agccgctatt tacagcagct gacgggtact
    54061 taggaatcgc tattatctgg ggagcgatgg cgctgttttg gtttgtcggt gttcatggac
    54121 cgtcgatcgt ggagccggca attgcggcaa ttatctatgc caatgtggaa accaatctgc
    54181 aattgttcaa ttccggtgaa caagcttcta aagtactgac ggtgggttta gggaattttg
    54241 taggaactat gggaggaact ggggcaacat tggttgttcc ttttatcttc ctactctttg
    54301 ctaagtctaa gcagttgaag gcagttggga aagcttcctt tattccagta agtttcgctg
    54361 tcaacgaacc attgctattt gcggcaccca tgattttgaa cccttatttt tttattccat
    54421 ttttgttaac acctgttgta aacgtttgcc tgttcaagtt ttttgtcgat gtattgggca
    54481 tgaacagctt tatgtacgtt ttgccttggg caactccagc accgatcggt ttggttctag
    54541 gaacgggcat gggtatctta gcttttgttc tagcagcggt tctaatcgtc gttgacttcc
    54601 tgatctatct accattttgc aaagcatacg atgctgtctt agtagagaaa gaagcacaaa
    54661 aagcagaggt gatcgcaacc gaggaaggtg cttctgccga tgtacgtgag gaagtacctg
    54721 tggcagcgag tcaagttatc agcgatgcaa ataaagaaat caatgtgttg gtactttgtg
    54781 ctggcgcagg gacaagtgcg atgttagcta atgctttaaa aaaaggtgca tctgaatcga
    54841 aagcaccgat cagtgcaatg gcgggtgctt atggttctca ttacgaaatg ctgcaggatt
    54901 ttgatatggt ggtcttagca ccgcaagtca attcttacta tgatgatatc aaagaagaca
    54961 ccgatcgctt aggaatcaag ctggttgcga caaaaggagc agaatatatc ggattaacga
    55021 gagatcctga aaaagctgtt cagtttattc tgaaccaatt tcaataaagg aggagccata
    55081 atgaaaatgg gaatttggtt gcgtaaagct gttttatttg cagctgtgtt aggggctttg
    55141 ggggctgttt caaaaactgt tgaagcatct gccagcgaat ttattcgcgg agcagatatt
    55201 tcgatcctgt cagatatgga aaaatctggt gcaaaatatt atgaagacga tatttcaaaa
    55261 gatgccttag agattttaaa aaataatggt gtcaactatg ttcgactgcg tttgtggcag
    55321 gatccttata atagtaatgg agaaagttat ggagcaggga caaatgatct ggaaacgacg
    55381 attgaccttg caaaacgagc aaaaaatctg ggacttaagg tgctgctgga ttttcactac
    55441 agtgattttt gggttgatcc tgggaaacag aatcttccta aagcatggca aggtctgacc
    55501 tttgaagaaa tgaatacggc attgtacgac tatacaaaga acgttctgtc ggaaatgaag
    55561 cagctggacg tttatcctga tatggttcag atcggcaatg aactgaatag cggaatgctc
    55621 tggccttatg gcaagtcttg gggagaaggc gggggagaat ttgatcgatt ggctgctttt
    55681 ttgaagtctg gtatccaagc tgttcgagac actcaaccta aaacaactcc agtaatgctc
    55741 cacttagctg atggtggcga tacaggagca tttacttggt ggtttgatga aatcacgagt
    55801 cgaggtgtgt catttgattt aattggagtg tcgtattatc cgtattggca cggttcaatg
    55861 aatgaacttc aaaccaatat gaatgcgatc agccaacggt atggtaaaga agtgatcgta
    55921 gttgaaacag cttatgggca tacaacagcg aatgccgata ctatgccaaa tgcttttggt
    55981 gaagccgaag cagctgcggg gggatatgaa ccaacaccga caggacaagc agagtatttg
    56041 ctggatcttg ccgatcggat tcaggcagta ccgaataatc gcggggcggg cttcttctat
    56101 tgggaacctc tttggtacaa cggaaatgtg agttgggcaa cacaagcagg tatgaattat
    56161 ctcagtgttc aatccactat gggaaacgaa tgggacaacc aagcgatgtt tgattttgct
    56221 ggaaacgcct tgccaagctt gcgtgcgttt aaacaagcag gggcgcaaat aaatctagtt
    56281 aaaaatgcaa gtttcgaaag cgatggacca acgaccagtc cttccaattg gcaaacctgg
    56341 tttcaacaag gaaccgctaa gggtacgatc aaaacggaag cgggcacttt ttttggggac
    56401 tacaaactaa ctttttggca ggatcaagca tacgaggcat cagcttatca aaatgttagt
    56461 ggcataagca aaggaactta cacattgagt gcctatgtaa tgtctggggg agagtttgat
    56521 actaatcagt tatatatcaa aaattatggt ggagcggaac taaatactgc ggttgttaaa
    56581 acaagtgaat ggacaaaaat tacaatcaaa gacattccaa ttacaacagg tgtttgtgaa
    56641 attggggttt atagcaaggc ttcaggaaat gcttggacga acgttgattt tgtttcattg
    56701 gtgaaacaat agactgcgga tcgtttcgat tcaagatcgg aggttccctt cagccacatc
    56761 gttagcgtag aaggcaggaa tcgaacctgc acctcgcctt tgccaaaaat aaccagaaac
    56821 gatttccagt catttttacc aaaacgagta aagcaccaga cttcatctac actttgtatt
    56881 atgctaaaga gagttcaacg atttcgtaag aagcaatgaa aaaattgcaa aaagttgtgc
    56941 agataacata aagtttgttt aagaaagggt aaccttagaa ttccgtcgtg aatatgattt
    57001 gagcacttag atggatagaa aaaagttctc ggaaaacagt tcgtttttcg agaacttctt
    57061 tttatgataa gaatgaaaca aattaactta tattatagtc tcttttggta gggaaccatc
    57121 ttatttttaa tgaaaatcaa gcattttagc tattgaagaa tgttgattta tagggattcc
    57181 ccctccatat tatgttggct ctttattccc actctgtttt caacgtaaag tgtataaaac
    57241 agaatgcgat actatcaagg gagttaaaac aatatacaga acagtaaaac actataaata
    57301 aaaataaaag tgcaataaaa gtgcaatttt tgcacctaat catactctgt ttatatatga
    57361 aaactagatt ctgaatccaa tatttggtta acgagaggcg ttcgaatttg aacgtctttt
    57421 atatattact ttcaaataaa tatgaggtag aatactatct attttctttt taacccaatt
    57481 gttataatgg agagtgtaaa atattttgtg taaatagaaa aaaggaagtc ccttctgtag
    57541 aatagagtta ccacaacaca ttcacagaag agaggacttc cctatgaacg attttactac
    57601 agaaattcta aagactctag cgaacaaagg cgatttgaat gaattattcc gtgtccattt
    57661 ggagaaagca gtcaatacgc ttctcaaaac ggagttaacg gctttcctag attacgaaaa
    57721 gtatgatcgc attggtttta acacgggtaa ttctcgtaac ggctcctatg accgtacggt
    57781 caagaccgag tatggggaac ttcatctcca gattccgcgc gaccgcaatg gtgagttcaa
    57841 gcaacagact gttcctgctt atagacggac gaatgacacg ttagaggaga ccgtcattca
    57901 cctcttccga aaaggtatta ccatgtcgga aatcgcagac ttgattgaga aaatgtatgg
    57961 gcattactac acgccccaaa ccatgtccaa tataacaaaa tcatttacag aagaggtaac
    58021 ggcgtttaat gggcgggagc ttcatgaccg ttatgctgct atttatatgg acgcaacgta
    58081 tattccgtta aagcggaaaa ccgtcgccaa ggaagctatt catatcgcag ttggcattcg
    58141 cccggacgga tcaaaggaag tattgagcta tgcgattgca ccgactgaat ccatcacgat
    58201 ttgggaggaa attttattgg accttcaaga gcgcggtttg aaaaatgtcc tcctgttcat
    58261 cacggatggc ttaaagggga tggtaggagc gattagtcgg ttctatccca aagctcgttt
    58321 tcaacattgt tgtgtacacg tttcccgtaa tatcagtcat aaagtgcgtg tcgatgatcg
    58381 taaggaagtc tgtgatgatt ttaaaatggt gtatcaagcc tcatctaaag aggtggcgtt
    58441 ggaagcacgt ggtacttttg cggaaaaatg gaaaaccagc tatccaaaag tggttgaatc
    58501 gattctttcg aacgatcact tgctcacttt ctatgatttc cccttggcca tacgcaagag
    58561 tatttattct acgaacttga ttgaatcctt taataaacaa atcaagaaat acagccaccg
    58621 caaggaacag ttccaaaatg aagagtcgat ggaacgtttc ttagtctcgt cttttgatac
    58681 ttacaaccaa aaattcctag gtcgcagtca taaaggtttc caacaggccg aaggcgagct
    58741 cgaacaaatg ctaagccaac tgattgagaa ttagaaacaa tctgcagtag ggaagaatca
    58801 tttacacaaa attattgacg ctcccttata atgagaactt ttactaacat aatttatgat
    58861 aaacttacta tataatcata ttaattaaat agttaggaga tctgcttatg tttatatcta
    58921 ctcttcaaat tcgaaatttt agaaattatg aatccgaaat ctttcgattc gataatgaga
    58981 caaatactat tataggtgag aatgattcag ggaaaaccaa tgctcttact gcattgagaa
    59041 tcttattaga tgatagttat tactatagta gtaaaacact aaaagaatca gatttttttc
    59101 atggaattca aaatgggtgg caagggcatt ggattattat ctctgcgaca tttgaaggaa
    59161 tcagcgaaga agaatttgac aatgagattt gtgcttccat ttctttaaat tctgaaagtc
    59221 aaacaatctt agaagaattg atttcaaatg cagataaagg tgtaggtagt atttctctat
    59281 ttattagacc taacaaagct attagaaagc aattatttga tatttccact gaattagact
    59341 ctcatcaatt taacgaattt agaaattcta ttcgtctatc cgactatgaa ttttactaca
    59401 catctaaatc aaatctaaat ttctgtatca atgaaaatta tgataaattg gtaggaagac
    59461 tcaatgacaa tatagctagt aatcctgaag aagacgatga agctctgttg ggctcaagaa
    59521 ttgacatgtc agatattttc aagcatattt cggttgttta tgttgacgct cttcgagatg
    59581 ttcttagaga aatgaaaaac aatcgaaacc cagtcaaaag aatcatggaa acaattgaat
    59641 caaaaatatc atcagataat gttgagtcat taaaaacaat tatacagcaa ttaaatgaga
    59701 ctattaccag tgttcctgaa attaggaaaa ttggcgaaaa tattaatcgg caactaaata
    59761 gcataattgg ttcagtgtat tctcctaatc ttcttctttc ctcaactatg tcagatgata
    59821 tgggttcttt agcaaaattt ctttcaatga agccagaaca aaatatcgac ttagacttac
    59881 tagggttagg acatctaaat atgatttatt tagctttgaa aatagtcgaa tttgaagcgt
    59941 gtcgttcaag agaactactt aatattatgt tgattgaaga accagaagct catattcatc
    60001 atcatattca aaaaactctg ttcgaaggat taaatcttca aaaaaactat actcagattc
    60061 tcatgaccac acattctgta catttagctg aagtgtctga aatatctcgg atgaatgtat
    60121 taaaatcgtt taatggcaaa tctatcgtta tgaatccagc aaataatctg aactcatttg
    60181 ctaaagaaaa gttaaaaaag aacaatctaa atctaataaa cgcagtagag agatatttag
    60241 atgttaaaag aaatggattg cttttttcta aaggtgttat cttagttgaa ggagatgctg
    60301 aagaaatact tattcctcaa atagtaaaaa aattatttgg tataaactta gacgaacttg
    60361 gaataggttt gattaatgtt ggtagtactt cgtttgaata tatcgctagc ttgtttgacg
    60421 atgatagaat acagagaaag tgtgctattg taactgattt agatgaacag gcaatcccat
    60481 cagaacatcg attgtataaa agcactgcac aagagaaagg tcaagcaaga aaagaaaaat
    60541 tatcctccct ttttaa
//
LOCUS       DACDTO010000008        56454 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDTO010000008 DACDTO010000000
VERSION     DACDTO010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.008"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            209..595
                     /locus_tag="IWK70_000535"
     CDS             209..595
                     /locus_tag="IWK70_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9613108.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            689..880
                     /locus_tag="IWK70_000536"
     CDS             689..880
                     /locus_tag="IWK70_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9613109.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            1394..1726
                     /locus_tag="IWK70_000537"
     CDS             1394..1726
                     /locus_tag="IWK70_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9613110.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            2022..2177
                     /locus_tag="IWK70_000538"
     CDS             2022..2177
                     /locus_tag="IWK70_000538"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9613111.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            2453..3793
                     /locus_tag="IWK70_000539"
                     /pseudo
     CDS             2453..3793
                     /locus_tag="IWK70_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4034..5650)
                     /locus_tag="IWK70_000540"
     CDS             complement(4034..5650)
                     /locus_tag="IWK70_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9613112.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            5768..5986
                     /locus_tag="IWK70_000541"
     CDS             5768..5986
                     /locus_tag="IWK70_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9613113.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            6181..6642
                     /locus_tag="IWK70_000542"
     CDS             6181..6642
                     /locus_tag="IWK70_000542"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9613114.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(6835..7074)
                     /locus_tag="IWK70_000543"
     CDS             complement(6835..7074)
                     /locus_tag="IWK70_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613115.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(7098..8621)
                     /locus_tag="IWK70_000544"
     CDS             complement(7098..8621)
                     /locus_tag="IWK70_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9613116.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(8639..8761)
                     /locus_tag="IWK70_000545"
     CDS             complement(8639..8761)
                     /locus_tag="IWK70_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9613117.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(8791..9774)
                     /locus_tag="IWK70_000546"
     CDS             complement(8791..9774)
                     /locus_tag="IWK70_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9613118.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(9798..9947)
                     /gene="rpmG"
                     /locus_tag="IWK70_000547"
     CDS             complement(9798..9947)
                     /gene="rpmG"
                     /locus_tag="IWK70_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9613119.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(9968..10345)
                     /locus_tag="IWK70_000548"
     CDS             complement(9968..10345)
                     /locus_tag="IWK70_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613120.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(10377..10556)
                     /gene="rpmF"
                     /locus_tag="IWK70_000549"
     CDS             complement(10377..10556)
                     /gene="rpmF"
                     /locus_tag="IWK70_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9613121.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(10571..10840)
                     /gene="rpsN"
                     /locus_tag="IWK70_000550"
     CDS             complement(10571..10840)
                     /gene="rpsN"
                     /locus_tag="IWK70_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9613122.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(11363..13135)
                     /locus_tag="IWK70_000551"
     CDS             complement(11363..13135)
                     /locus_tag="IWK70_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613123.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(13089..14843)
                     /locus_tag="IWK70_000552"
     CDS             complement(13089..14843)
                     /locus_tag="IWK70_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613124.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(14953..15522)
                     /locus_tag="IWK70_000553"
     CDS             complement(14953..15522)
                     /locus_tag="IWK70_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9613125.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(15540..16964)
                     /locus_tag="IWK70_000554"
     CDS             complement(15540..16964)
                     /locus_tag="IWK70_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9613126.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(16966..17634)
                     /locus_tag="IWK70_000555"
     CDS             complement(16966..17634)
                     /locus_tag="IWK70_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9613127.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(17764..18348)
                     /locus_tag="IWK70_000556"
     CDS             complement(17764..18348)
                     /locus_tag="IWK70_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9613128.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(18679..18912)
                     /locus_tag="IWK70_000557"
     CDS             complement(18679..18912)
                     /locus_tag="IWK70_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9613129.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(18927..19877)
                     /locus_tag="IWK70_000558"
     CDS             complement(18927..19877)
                     /locus_tag="IWK70_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9613130.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(19874..20716)
                     /locus_tag="IWK70_000559"
     CDS             complement(19874..20716)
                     /locus_tag="IWK70_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9613131.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(20718..21437)
                     /locus_tag="IWK70_000560"
     CDS             complement(20718..21437)
                     /locus_tag="IWK70_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9613132.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            22129..22287
                     /locus_tag="IWK70_000561"
     CDS             22129..22287
                     /locus_tag="IWK70_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9613133.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            22359..23585
                     /locus_tag="IWK70_000562"
     CDS             22359..23585
                     /locus_tag="IWK70_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9613134.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(23673..24065)
                     /gene="rpsI"
                     /locus_tag="IWK70_000563"
     CDS             complement(23673..24065)
                     /gene="rpsI"
                     /locus_tag="IWK70_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9613135.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(24081..24524)
                     /gene="rplM"
                     /locus_tag="IWK70_000564"
     CDS             complement(24081..24524)
                     /gene="rplM"
                     /locus_tag="IWK70_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9613136.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(25043..25510)
                     /locus_tag="IWK70_000565"
     CDS             complement(25043..25510)
                     /locus_tag="IWK70_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9613137.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(25630..27168)
                     /locus_tag="IWK70_000566"
     CDS             complement(25630..27168)
                     /locus_tag="IWK70_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9613138.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            27248..27934
                     /locus_tag="IWK70_000567"
     CDS             27248..27934
                     /locus_tag="IWK70_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9613139.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(28135..28662)
                     /gene="msrA"
                     /locus_tag="IWK70_000568"
     CDS             complement(28135..28662)
                     /gene="msrA"
                     /locus_tag="IWK70_000568"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9613140.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(28746..29423)
                     /gene="pgmB"
                     /locus_tag="IWK70_000569"
     CDS             complement(28746..29423)
                     /gene="pgmB"
                     /locus_tag="IWK70_000569"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9613141.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(29420..30550)
                     /locus_tag="IWK70_000570"
     CDS             complement(29420..30550)
                     /locus_tag="IWK70_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9613142.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(30550..32733)
                     /locus_tag="IWK70_000571"
     CDS             complement(30550..32733)
                     /locus_tag="IWK70_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9613143.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(32730..33755)
                     /locus_tag="IWK70_000572"
     CDS             complement(32730..33755)
                     /locus_tag="IWK70_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9613144.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(33800..34627)
                     /locus_tag="IWK70_000573"
     CDS             complement(33800..34627)
                     /locus_tag="IWK70_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9613145.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(34624..35691)
                     /locus_tag="IWK70_000574"
     CDS             complement(34624..35691)
                     /locus_tag="IWK70_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9613146.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(35717..36553)
                     /locus_tag="IWK70_000575"
     CDS             complement(35717..36553)
                     /locus_tag="IWK70_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9613147.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(36550..37419)
                     /locus_tag="IWK70_000576"
     CDS             complement(36550..37419)
                     /locus_tag="IWK70_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9613148.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(37489..38772)
                     /locus_tag="IWK70_000577"
     CDS             complement(37489..38772)
                     /locus_tag="IWK70_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9613149.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(38786..40471)
                     /locus_tag="IWK70_000578"
     CDS             complement(38786..40471)
                     /locus_tag="IWK70_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9613150.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            40746..41771
                     /locus_tag="IWK70_000579"
     CDS             40746..41771
                     /locus_tag="IWK70_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9613151.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(42366..46019)
                     /gene="rpoC"
                     /locus_tag="IWK70_000580"
     CDS             complement(42366..46019)
                     /gene="rpoC"
                     /locus_tag="IWK70_000580"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9613152.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(46098..49715)
                     /gene="rpoB"
                     /locus_tag="IWK70_000581"
     CDS             complement(46098..49715)
                     /gene="rpoB"
                     /locus_tag="IWK70_000581"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9613153.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            50443..51411
                     /locus_tag="IWK70_000582"
     CDS             50443..51411
                     /locus_tag="IWK70_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9613154.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            51429..52334
                     /locus_tag="IWK70_000583"
     CDS             51429..52334
                     /locus_tag="IWK70_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613155.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            52331..53137
                     /locus_tag="IWK70_000584"
     CDS             52331..53137
                     /locus_tag="IWK70_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9613156.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            53294..54262
                     /locus_tag="IWK70_000585"
     CDS             53294..54262
                     /locus_tag="IWK70_000585"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9613157.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(54579..55103)
                     /locus_tag="IWK70_000586"
     CDS             complement(54579..55103)
                     /locus_tag="IWK70_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9613158.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            55390..>56454
                     /gene="scm"
                     /locus_tag="IWK70_000587"
     CDS             55390..>56454
                     /gene="scm"
                     /locus_tag="IWK70_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9613159.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        17056 a        11425 c         9621 g        18352 t
ORIGIN      
        1 tttaagaaga tatatgaatt ttcttttcta agtataaaga taactatcca ttatcaacta
       61 aaaaaacatt tttgaaaata aacatcagca gtcctatcaa tgcatgtgag cttatagaag
      121 ttattcgcag cgcagcataa ttgcattagc ttcttttcgt gttttttgcc tcttttccct
      181 ttaaatctta atggaggagg tgtgcaatat gtcagataac gacgaacgcg taaatgccca
      241 gttgattcga ttctttgaaa aggtcatttt gaacaccgct aatacttatt ataaaaaaca
      301 agaagaaatt caaaaacatg aacaacttga tcaactccca ccatatttgt ttactaaaga
      361 tactataaaa tttaaacaac cgattaatgt tttaaatgtg actctccttg ttgaagatat
      421 aaaattagca aaaatccttc ctttgttgaa agaacctgaa caaatttttc ttatcgaaaa
      481 atttatcttt gataaaactg ataaagaaat tggaaaggtt ctggggatta caagacaggg
      541 tgcgacaaat ttaaaacatc gactttacaa aaaaattcaa aaaatgctgg tttaatatct
      601 gcataataac taattttgta tgcttctttg ctcgttctcc tccttcccaa agaaatagat
      661 tataagtcca tgggaagaag gtagatttat gaattcttta aaagaagatt tcattttagc
      721 aaaggctgga aatgaagaag ctgttgaggc aattctcaaa agatttagct ctttgattca
      781 taagcaatcc tggagaactg gaaaatatga tcaagattgt tatcaagaat gtatgcttgc
      841 aatatatcta gctatctcga aattcgaaat taaagaataa ctcaactaag ggagtctgtg
      901 acataagtaa aaagatagtt tcaaatttcg aacaataaat ttgaaactat cttctttata
      961 attataaagt atcgatttta cagcgttcta taatgattca tagaatctaa aacgaatgat
     1021 tttaattatc aacgtttttt aattcgtttt tagcttaaaa ataacttttg tcacagtctc
     1081 actcctaaaa ttactttatt tacgtatgaa gtgggcagga ggtcgaatcc agaactgggc
     1141 tatggtactt caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt
     1201 aacgacggta gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag
     1261 tcaagagaat tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa
     1321 accctgatac taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa
     1381 ggaatgaact ctattgagaa aaacatatga tcgtaaattt aaactaaaga tttcacagga
     1441 tatccttgag aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc
     1501 aacaatttcc cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca
     1561 aggaaaaaga ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact
     1621 aaaagaggaa gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact
     1681 caaaaacttt acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt
     1741 gaaatgaatt tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa
     1801 acaatcaaag aaatggtaaa aaaatgttga atcgataatt tgaacagaac caaattgatg
     1861 ctgtttgggt gatggatatt acttacattc catgtaatga cggtcgcctc tatttgtcca
     1921 cctatattga cttagcaact cgtattctac gttactatat ggtagattca cacatgaaaa
     1981 aagagattgt cgtccgtcca ttacaaattt attaaggaga attgcctacg gtaattcatt
     2041 ccgatagagg tagccagcat cggtctttta tttatcaaga gctgttgaac gaccaccaca
     2101 tcacacattc agtgagccaa actgggactc ctgttgacaa tgcagtaatt gaagcgtttc
     2161 atcgatcaat caaatgagaa ttaatcgact ccaacaaata taaaagtaaa atagagatga
     2221 aggtgttaat ccaagattat ttagaagact actacccaaa caaaagaatc catacaaaat
     2281 ttatgatgac tccttgacaa tttgaagcga agaaaataag ggatctataa tatctagtgt
     2341 tggtgacgcc ttctgttgcc aatacttttt cttttttcta ataaacgcaa acagacacct
     2401 taaaatcctt tagaataaag tcgacgaaaa ccattcaaag gagtgtttca agatgtccta
     2461 ttcagagttt accaaagagt tactagatat tctagaccta aatcttatct ttcatgaaga
     2521 tgcctttcgc aaagagcgaa tcaatgacga aacatgtttt gtttttgatg gcacattgac
     2581 ctatcaaccg gaggaatgct ttcatcgcca ctatcaaaat gaacaaacga tcattaaata
     2641 gggctggaaa aaagtatcca tcttattgaa tgatgtcagt aactacaaga ccattctgcg
     2701 gattaacaaa cagcgcttca aatgtaaaca ctgtggaaag acttttttag cagaagattc
     2761 agttagtgat cgtcgctgct ccattgctcg cagagtcaaa caagccattc ttgagctatt
     2821 aagcgaacct ctttctatgt ctttgatcgc acgtatgaag cacatttctc cgaccactgt
     2881 cattcgaata cttcgcagcc ttcggcctaa aaccatttct ttgagccagc tgttaccaga
     2941 agtggtgtgt ttcgatgaat tcaaatcagt gaaaaatgtc tcgggagcca tgagctttgt
     3001 catgatggac ggcaagaccc atcaactgat tgatattgtg gaaaatcggc aactcaatcc
     3061 tttacgcgac tatttcttgc gctaccctat aaaagtccgt gaacaagtgc gtctagtggt
     3121 ttcagatttc tatacaccct atcgcacatt ggtgaaagag tgttttccga atgcccgaat
     3181 cgtcgccgat cgctttcata ttagccaaca cattggtcgg gcttttacca accaccgaat
     3241 tcaagtaatg aagaccttta aaaaagggga tcgtcggtcg aagcacttaa agaaatactg
     3301 gaaactactt caaaagaatg cgtgggaatt gaaaggtcag catcgctact ggcgtccgtc
     3361 atttcgagat catctgacag aagcagagat agtcgatcgg ctattatctt atgatgattc
     3421 cttaaagcga ggctacgaag tctaccagga ttttctttcc gttatcagaa gacaagatgt
     3481 tcctgaattc gtcgcgctcc taaaagaaga ctacaaggag ttgccagagc actaccaacc
     3541 agtgttcacg accttcaaga aataccaaac agaaatcaaa cgagcgttgc gcgttcccta
     3601 ttccaacggc cctatagaat gtttgaataa ccatatcaaa gtactaaaac gaattgccta
     3661 tggctataga aactttcaaa actaccgaga acgaatcttt ttatatcgag gaaaatattt
     3721 taagaaaact aagaatacta cccaactgac taaagccaga acaacaacac ggctagacaa
     3781 gatagctgtc tagccgtgtg agtatctcga ttattctatt gaatttcttc accaacactc
     3841 attgacgaag agccaaaata agtaatctaa ccgactgtta actaaaccat tgcaggtccc
     3901 gttaaagata cttgtgcctg ttgtcaccgc ttctttttct tgtccacaag cgcttaacac
     3961 acttaataaa gcccctgcta ttattaataa taatatcttt ttcttattaa aaacctcctc
     4021 atctatttcc agattagtaa atgtagtaat ctgtgctttc ttttttctca atactaataa
     4081 ctaacttatg gggtaaacaa atactcgttt gaccgggttt tgagatccat ccagttctaa
     4141 ccgcgatttg atcaggacta ttatcttctt taactcgaat tcttccatct ttaatctcaa
     4201 caatattata ttgtcccttc gcaggatgat aattcacgag tttatgattg atcttatcca
     4261 aatcgaagcg atccatttcc ttgccatcaa ttcgaacgat ggcatatttg atagcatctt
     4321 cagttgtttc attctccttt tctatagaga atacaaataa gggaataaaa gaggtaataa
     4381 tcaaaatgcc aataattatg acatcgccta tcttgacacc agcttttctt aaaaatttca
     4441 ttaacgatat ctggctaaac atattagact ttttccatgg aaatggtgaa atttttcgga
     4501 aattatctgc attaagagaa tcagttaaac aaccctcaag ttgttcctta atacattgtt
     4561 gatccatctt cataccaata aaaactagtt tattaatgcg ttcaccaaac aacggatgcc
     4621 aggcttcctt caattcggga tttttttgta taatcatttg acactcaatt ttaggtaaac
     4681 tagctaccca ataatttaat gattcaatac ttttatagct acccgatgtt tcaaagatcg
     4741 tcgaaaaatc actatgttgg gcaaaccaaa gaaacccttt tgctcttgta atattgccag
     4801 gaaaattttt atccgtaaga tgggcaaatt tttgtgcgtc aaggagtaac cgatttcgat
     4861 agataaaact tgagatacca tattcttctg tttcaggaat atgattttga tagccaagct
     4921 ctaactcttt gaaccagccc actgaatagg aagtctcatc tatattaaaa agatttgtat
     4981 ttagaatttt cgataagtct acttcacctt taattgtcct aattattttg gcattggact
     5041 gtaacttagc aattagaccg ttaattttgt aaatcgtctt ggttgacact aagtcactat
     5101 tatttaaaag taatatgtta caacactcga attgatcaat taacaagcca cgaatatcct
     5161 gttcttcttc aactacatta ccttcagctt ttctttcctg aatcttttca cccgtttcga
     5221 aattatccca aaaacgtttt acatccacta ctataataat ggcaccaatc tctaatcgtt
     5281 gggtcaaatc aatgtctaaa gggtttttag ctaatataat tgcttgaata attggaattg
     5341 gtttgctgat tcctgaagct tcaataacaa tttgattcaa gtcagaaaat tgcactaatt
     5401 gatctagttc aacaatcaaa tcttcgcgta atgtacaaca aatgcatcca ttcggcatcg
     5461 aaacaagttt ttctccagtt cttttaaaac ctttcttgtc gactaatacc ccatcaacat
     5521 ttacttcccc tatgtcattt acaatgacag caatttcctt tccgtgatca ccgtacagaa
     5581 tatgatttag aagtatcgtt tttcctgaac caagatagcc agttaagatt gtaataggta
     5641 tttttttcat agggaacctc tttctatacc ttgaatttac actttaagtg caacacataa
     5701 aaatgagtca taatcgatat ccaactagta taagtttgtc gagaacactc ctagaaggaa
     5761 aataattatg agccagtatc gtcatcttac tatactcgaa cgagaaagaa tctttctgct
     5821 tcacgaagaa ggattttcta ttagtataat tggcaacgac attcagcata gcccgtttac
     5881 tattttacat gaactgagaa gaaacaagaa aaaaactatt caccttccaa agcggaaaaa
     5941 aattatgctt atcgaagaaa aaaatgcgga cgttatcaca ttctaaatga tccgtgagtt
     6001 tggtcaattg ttcgtcattt atttgtcgat tttcagtggt cgccagagga aatctcgaat
     6061 cgtttgaagc atgaacatat ggacttttca attagtttta tcacgatcta acgtgcaatt
     6121 tgcaatgatc ttttcgatac agaacccctc tcacacggca ataaaggcct gatccgttca
     6181 ttgcaccatc gaggaaaaac aagacataca aaaaatcatg tcgagtctcg tggaaagatt
     6241 tgccttactc attcgatcca tggccgtcca aaatccgcag atgatagaag tgattttggt
     6301 cattggaaag ccgatattgt agctggcaaa actggtggta cttgtttagt gactttgaca
     6361 gacagaaaat catgcttcct tttagcagga aagaaggtcc ccagaaagaa agccgcttat
     6421 gtgagcgaga aaatgattga acttttaagc agtattccaa atgaggcggt caaaacgatc
     6481 actccagata gaggaaaaga attctccaat catttcgcag ttacagaagc attgaataat
     6541 atgccctttt attttccaga tcctcatgcc cttggcaaca atgaacaaat taaaatacga
     6601 atagactcat tcgagaatac ttgccaaaat cgaaagagat agctacggta gataactcaa
     6661 cgataaatca gatgattgaa aaactgaata acagacctag aaagtgttta gattggagaa
     6721 caccttatga agtcttcttt gataaagtgt tgcgcttgat ttgacaattc aagacataat
     6781 aaaaagcaga tcaaatccaa ataagggaaa aatgatctac tctggtatta tcaattaatt
     6841 ggccaagcta ttatgccgtt ttgctaaacg tctacttctt ccagtattga ccttgataaa
     6901 acaacaatca ggatcccgtt ttaatactct atgaactgtg tcaatactaa catgtaaagt
     6961 tgcagcaata tcagctaatg tactcccatt taatctcatt tgctttgcaa atcgataacg
     7021 ccgataagca tcaatattct tggaatccat agacaataat acttttctta ccatgtattt
     7081 cctccaaaaa ttgaatttta atgagccatc atttcttgag caatttcatg tttagacaat
     7141 gaagctggat agaaagtagg ccaattatgc aattcattat agagtttttc ttgactttct
     7201 ccaccaaaga aaatatggaa atgctctgcc ttacttggtg caattccatg atcactaaat
     7261 tgaatatatt taaaacgacc agcacctgca tcatcagttt caaaattaaa acgaacacca
     7321 cggtttcctt tttcataatt caaaattttg tatccaacat atttgtatgt gtattgatga
     7381 tgttcaccgt tgactacaaa atcaatggtg ttgtctttaa tgttgatagt atcaatatct
     7441 gtcttatagc cagtagtata ataatctttg tactcggcag ctgtcatgtc tttattgatt
     7501 tttgctttgt aatcgaaaac ttcatccaaa attccatctt ttaacaacgg ataaacagat
     7561 tgccattcgc ctgtataatc actcaattcg cgatccttca catcggcatc tttaaagtac
     7621 ccattagcaa ctgtcttttc atccttcgct tctttttctg gaactacctc ttcaccagca
     7681 accatagttg ttttttctaa cgcttttaga ttgtcttcca ttactgaaac ataatcttca
     7741 ccattatcca tttgttcttt tgtaagactt tcaagaggat tcagtacttc caattttatc
     7801 ccagcttcgt tggccaaagt ttttgcaatt ttatcattag cattcttttc aaagtaaata
     7861 taattgatct tatttttttt aacatactct tttaattcag ctaatcgacc agaagatggc
     7921 tcttcttcag gacttagtcc agcaatagga acttgtttca atccataatc caaagctaaa
     7981 tatccaaagg ctgcgtgttg cgtcacaaaa cttttttgtt tcgcttcttt aagactagtg
     8041 gtatagtcag catccaaccg tttcagttta gtcaaatact tttcagcatt tgtctcaaat
     8101 acttttgctt ttttcggata caatttaact aactgatcct tgatatttga aacttcctta
     8161 attgccattt tgggtgaaac ccacgtatga ggatctagtt catgatggtg atcttcttct
     8221 ccgtgatcat gatcttcttc ttcactacct ggcaataaaa tcatgtcttt tgttccttct
     8281 actacattag gcccagcttt cttccaactt tctttagctt ttggtaccca tgattccatg
     8341 ttttcgttat gatatacaaa aacatcagca tcatgagctt ttgccatatc cttagctgag
     8401 ggctcatagt catgaggttc tgatcccgct ggaaccatta aatccacgtt cgcttcatcg
     8461 ccgacgatgt ttttggtgaa atcatacatt ggatagaatg ttgttacaat ttctaatttt
     8521 ccatctttat cattcgatac ttttgcatca ttagtgctgg agcaagctcc caataatcct
     8581 agagctgcta tcccaataag tcctacactg attttcttca ttctttttcc ttccttaatt
     8641 atgctaattt cttcatttta tttcgtttat gttctttgat aatttttaat gccctttttc
     8701 tagttttaat attcgggctt ttaagtcgtc gatatgcact agatatatcc gttttctcca
     8761 ttagaaatta cccccaatca ctacttatca ttatttgctt tccgccagta atttatcaaa
     8821 actctgtttg atcctttctt catccaacat ttttccgatt agaataatag tggtttggtc
     8881 tttttgtcga gtggtgtatt gaaaattaac ctgttcgttt acctcttgca tggcaactat
     8941 aaaatcctgc tcttgtaaag caatcaatcc tttaaaacga tacaggctcc cttggttggt
     9001 taaaatcagc cagtcaatcc attggagtaa aaaatctttt tttatgtctt gatctgaagt
     9061 taagagaatt gatttaaact catgtacttg atggtgttga tgatagtatt tgccattata
     9121 gtgattttct gatgaatcac tttcaaaaac taagggatga ttaaattttt tcaaattata
     9181 aaaatccttt ttttgaatcg tttcactatg tgtgaacatt cgaatatcgg ataaagggtt
     9241 aatacttttg atttctctct gtaatttttt tagtgcagat aaattcaagt tttgttttct
     9301 agacacaaaa atgcgatctg ccatgacaag ttgcttaagg gcttgtggtt ctctttcaat
     9361 aagttcaaaa ctgtcactat caataacagt caataaacta tcgatatata ctcttcctct
     9421 tagttgaggc gctgttaaaa gtgtttgaat gattggttgg ggatctgcta tacctgtagt
     9481 ttcaatgatg atttgtgaaa ttggttgttg atgttcttct attgtttcca gtaaagaaat
     9541 caatattgct aataaatcac tgcgaagact gcaacacata catccattat ttagttgtaa
     9601 cacccgttcc tcgctatgaa ctaataattt gtgatcaatc ccaacttcac caaactcatt
     9661 ttcaattata ataatttctt ccctggggta gggtgagact tgcaaggctt gattgattag
     9721 cgttgttttt ccagccccta aaaacccaga aacgattgta actggtatgc ccattttgtc
     9781 acctttttac cacttagcta tttcacttct ttaaaaattg cccgtctgcg caattttggc
     9841 gaatactttt ttaattctaa ccgttcaggg ttattccgtt tatttttgct ggtgagatac
     9901 agacgttctc cagtttcgac acattccaaa ataatatttt gacgcataaa tcttcctcct
     9961 tcaatactta tcgatcttca agatatgctt gaatttgttg tgcatcttca aaagttaact
    10021 cgccagattc caattgtgaa actaaatttt ctttcatatc aacaggtatc tcccaataac
    10081 catcgataca ttgaaataac tcctcgtcat actcttcaaa aattggatag attcttaaaa
    10141 aatcatcaaa tgataagcct tggtcctcat agtaatcttt gactaatcca ttttcaattg
    10201 cctgttgggt cggtaaaccc gtttcagtaa taattcctct ctctaccatc gaacgataaa
    10261 aagtgcattt atctagcgat tgctgggtcg tttcaagata atactttaat ccttcttcaa
    10321 ctaaaggtgg gtaaataatt tccattttga ttccctccta aaagttatat attaaactat
    10381 tttgaagctc ctttaattac tttctttcca ttatagtacc cttttaaaga tacatgatgg
    10441 ctacgtctat aatcaccatt cgcttcgtca aatgaaattt ctggttttac tacagtttga
    10501 tgagttcttc ttaaccgttt cttcgcctta gatgtttttc ttgctggtac tgccatgata
    10561 tttcctcctt ttaccaactt gcttttttta ctccaggaat caacccttga tgggcaagtt
    10621 ctctaaactt aatacgggac ataccgaact ttcgcatata ccctcttgga cgaccatccg
    10681 tctgatcacg aagcctcaga cgattcggat ttgaatctct tggcaattta gcgagagcgg
    10741 tgcgatctcc tgcggctttt aattcctgac gtttatctgc atacttttca atcatcgcca
    10801 tttgtttctt tgctttagca attttcgact tttttgccat atacctttat actcctttta
    10861 ttttgattta gacacctcaa atcgtaatcg ttacgttttg acactgatac tttaccaaca
    10921 agagttgctc ttgtcaacaa taaataacat gaaataaaac tcatatgaat agataactat
    10981 ttgaaaacga atttatcggt acatagagat aatcaaatca tttaaaagaa tatgcagttt
    11041 aggctctgaa aaataagaat tcacttttat tagtccaatc aaacacaaat accgttttaa
    11101 gtagcttttc ctctacttaa aacggcaata tgaaagaaac tataactttt tattcaatct
    11161 tttatgttta actggtttat cgccaatttt ttaaaacttt gattattcct tatattataa
    11221 gaatagacat taagtgaact aattcaatca tactgattct ttcgtgtatt caaaattaga
    11281 tgctgtttaa taaaattatt ctttttgaaa aagaattata actatatccc aaaaattgta
    11341 ggttaattta aagcctattt catcatcgtg attgagcttc tttttcatag acgaatttct
    11401 cagatagttt agttttcttt atgagatttt tgtatatttc tgaccttcga tacaactcat
    11461 tatgagtacc ttgatcaact agttttcctt gatccatcac tacaatttta tttacctttt
    11521 caatagactt caagcgatgc gatataatta gtactgtctt atcttgtatt aaacgattta
    11581 aactttcctg aatacgtttt tcattttccg catctaaact ggcagtaatt tcatctaaaa
    11641 ttaaaattgg cgcatttttc aaaaaagccc gtgctatgga taggcgttgt cgctctcctc
    11701 cagaaagtga acttccgttt tctccgacca atgtatgata accgtcaggt agtttttcaa
    11761 taaagtcttc gcagttagct aaacgagctg cttttttcac ttcctcatct gacgcttgac
    11821 tatttccaat acgtatattt tccaaaatac ttgtattaaa caaagttacc tcttgaaaaa
    11881 caatagcgat tttgctaaac aaagaagctg gagatacacg tttaatatca tacccatcaa
    11941 ttaaaataca gccttcatca taatcataca gtctagacac taatttcaag atactagttt
    12001 tcccggaacc actagcacct actaacgcag tcacttctcc ttgtttagcc gtaaaagaga
    12061 tatgatctaa aattggggta ttgttatcat atgagaaaga gacatctcgc aactcaatgt
    12121 caaaagattt cagaggacta tctgatccct cttggatact ggtttccttc atagctctaa
    12181 ttcgctgtat tttaggagct aaataaaata tttcaagcac tgcttctttc atagaatcga
    12241 aggaatcctt tatttttatt gcggctagta agtaaccaac gacgtagagt atagtcactt
    12301 cacccgttaa cagtaagtga acacctacta gaatgactac tgctaaacta acatatgaga
    12361 aaatagagga taaggccatg actgaaaaag tacttaattc tacctttaaa tgaatccttt
    12421 cactctcttc catttttttg aaaagtctgt cttgaacttt tttagataga ttaaagctat
    12481 taatctcttg ctgcaattct atagtttctt gaaattcttc cgagttttct ctcaaagtat
    12541 cgtaatattt agtattggct ttcgtttggg atttctttga taacaagatt aagacaaaac
    12601 taaataacgt tggcaaaata acagctaatc ccattttgac attaccaacc aaaagcatca
    12661 ctgaaataaa agggaaaaac agagccatac cgccggcttt aggtattgca tgactcatcg
    12721 catgctcaat accttcaacg tcagacatga tagtttgaga taaatctgat aaattatgtt
    12781 ttgaaaaata ggataatggt aaatttgaca aatcgtctgc aatttgcact cttaaatgcg
    12841 cactttcttt ataagtacta ttatataagt tttcatactc cctatctaac aaccaataca
    12901 tgaaaagcaa agtaacaaac gatacggcaa aataaagcca acggggcttt gtattttcta
    12961 aaactaattc gtctatcagc atcattataa tgaatgctgg taccatatta atgcagtata
    13021 caaaaaaact agaaatagaa gctttagtta aagcttctgc gccctgatca cttaaagcaa
    13081 atttgttttt ataaaaactt ttcattgtca agcctccaat catttgcatt ctcataatta
    13141 gtgaccaatt gtttatacac ttgatgttta gccattaatt cattatgatt tccacgttct
    13201 actattttcc cttctgacaa gacaagaatt tcatctacag atttaatgct ggataatcga
    13261 tgagctatca taataactgt tttttctttc atcagatgtt gaaaagcctt ttgtagttca
    13321 tactcattat ctggatcaat agatgcactg gcttcatcca taataacaat ctttgcatct
    13381 tttaaaattg ctctggcaat agctatacgt tgtttttctc ctcctgacag ataaactcct
    13441 ttactaccaa ttaaggtatg ctctctttcg ggaaattttt ctaaaatcga ttcgcaaccg
    13501 gctaatctta gcgcgtcaaa tactttactt gctggcgctt gaggatttgc tatagaaacg
    13561 ttatcgtaaa tactctgatt gaatagtttc gtatcttgaa agacaaaagc aattgtgtta
    13621 ataattgtgt cttttgaata gtgatttaaa ggcttaccac caattttaat ctgtccctta
    13681 tccacattgt agaaacctga aattagttta gctatcgtac tctttccgct tcctgatttt
    13741 cctactaatg cataaattct tttttgttct aaaactaagt tgaaattttc taaaacacac
    13801 tcttctttgt atgaaaagct gacattttca aatgcaatgt cacaattttt aatctgtgtc
    13861 tcgttcccat attttagttg gtcttgcttc atttctaaat aaagggagtc tagtgtttcg
    13921 actgcatatc ttgcttcaaa caagtgcata cttacataca ttattctcat gaaagaaaca
    13981 aaaataattc cacttaaaaa aaacaacatc aacaagtcaa cagctaatat cttgggagtg
    14041 gtaagagaag ttataaaaaa ggttatagga actatcaaaa tgcaaactat gccaaaaaat
    14101 agccattgaa aaagaacaaa aggtgttttc ccactcaaag aatacttata tgcaaactta
    14161 gcataatcgt taattgcttt attcaaggct ttaaatgatg ttactttagc accaaatatc
    14221 tttataactt gaattccgcg gatatattct accgtttcag agcttaacac atctaacgat
    14281 tcttgatatt tttgaataaa atttgtattc tctgtcattt tctttaacaa gaatacacca
    14341 attactaaca tggcgattag aaccaatcca attttaaagt taatgaaaaa gcctaaggca
    14401 actgataaaa caggtgttac tatggcttga gcactatctg gaatcaagtg agcaattgcc
    14461 atatgtgttt tagccgcgtt gtcatcaatt gtctttcgaa tatatcccga agaatttaaa
    14521 tcaaaaaaac gaaaacttgc ttctgttaaa ccgctaattc ccttttttct caaattagtc
    14581 tctaagcgaa acccaaggat atgagaaaat aagccagaaa aaatataaaa taaagctcct
    14641 aaagttaacc aagcaacagt ttgaaccgca taactcttag ctaaatattc atctcctaca
    14701 ataattagag actgaagaaa tttgtaaata ctgtaatatc catagactgt taatacgaca
    14761 gaaaaatcag aaaataaagt ggctaaccat ccgtagtatt tcattttagg gacataacta
    14821 aataattttt tgtatacttt cattttttcc tcccccgcat tcaatttctt ctcttattcg
    14881 tttacaactg ttaatgcttt ccgtaatagc gtaaattaga gatgtacaaa cagataaaag
    14941 gtaaactacg ttctattttt tgtatttttt taagactttt cttccaatca atacaccaat
    15001 catagataaa cttgctacaa ctaacactga aatgattccc catataccta ccgcatagtg
    15061 gatcattgca tccattgttg cagcagattg tcctttatct atccaatctt gacgaaaacg
    15121 atctgtaaag aaccatatag gaatccactg tccggcagca ctgcctaatt gcattagagc
    15181 ccaagacgca acttgaggaa ttgctcgatc atatccaaat acagatagaa taatatcagc
    15241 taagatcgcc atactaatcg aactgataaa ccaaggtaag tacgccccgc ccattaacgt
    15301 aaaaaataac aataaaacaa ttgtgtaaat agtaaaaatt ccttttctag ggtagttata
    15361 gctaatataa acaaatatga ttccagataa taatccaaaa atcccggggg atatgctgtg
    15421 accaaacgcc ccaccaatac tactaaccat tattgaaaaa aagtaaatta ctagggaaat
    15481 aactgtagct aaaccaataa aaatataatc ttttgttttc atatatattc tcctcaattc
    15541 tatttcgtta tgtcattaaa aatctgttct atcaaagttg aatcttctac tctaagtttt
    15601 atatcttttt ttattccacc attttttaaa taaataattc tattgcatgt tttataaaga
    15661 aattccggat catgagtagc tataactact gcaccctttt cttttaagtc tttaataagg
    15721 tcagcaatcc tttccatatt aatataatcc aacccacttg taggttcatc aaatagaaat
    15781 aaattagttt ttgacaatga agctatagaa accaataatc tttgtttttc tccaccggat
    15841 aaatcaaacg gtaattgatc tttcatttta taaagttcca ttttttttaa aattttttct
    15901 tgctgatctt tactaactat ttcattcccc agggctaact cgctcctcac tgattctgtg
    15961 aaaaattggt aatccatttc ttgcattact aaaaacggcg atgacaaatt ttttttattt
    16021 tcgattatct ttcctttggt agggcgaata atcttcatga gtattcttag taaggttgtt
    16081 ttccccacac cgtttttccc taccaaacca ataacatctc caactttgac atcaaaattt
    16141 atgtctttta gaatattttc ataagtaatt tcttcaactt ttaatacatc tgcacttacc
    16201 ttttcgatat agggaacttc aatcgaacta ttaaaaatgt caaatttcct taatgcgtta
    16261 ttgttttcag tttttaacaa ctgaatagtt gtagttttta cagttctatt tttaacaaat
    16321 agaacttgat caattaattc tttcaaatag taaaaacgat gttcagctat aacaatagta
    16381 aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc
    16441 aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa actacatcca
    16501 atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc taatatattt
    16561 gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc ttttgtaaat
    16621 ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa gaattgtcct
    16681 ctaggatctt gacttacgta gccaatattt tttgatatac tttcttgtgt taaggattgt
    16741 agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag tcttggaata
    16801 agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc agtaatcaat
    16861 gtaagactgc ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc
    16921 ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa tcaaaatact
    16981 taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca tgtttgtttt
    17041 atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat tagcggtcat
    17101 ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt ccaaatattg
    17161 cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt taatcattct
    17221 tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta acattatttt
    17281 tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat agacactaac
    17341 caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata aggatgtcgc
    17401 aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta ctaaaaacag
    17461 aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca tcaatactaa
    17521 aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta aaaaaataat
    17581 gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg acactctttc
    17641 acctccaaaa aataagatcg cactgttatc ttaagctgat aaaaatgcac cgttattcag
    17701 ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc aagctttttt
    17761 taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa
    17821 tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa
    17881 tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc
    17941 gatagtcttt aacttatcta gacaacagac tgtctttttc gtcacaaatg atgtaatttt
    18001 ttcaacaata tcttgatatt tggttccagc tgattggaat aaaatcaaat cgaatatacg
    18061 tttattctca aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact
    18121 tttctctata tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat
    18181 attaataagc gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt
    18241 aaaatgttta taaaaagcac ccgtagttac gcctgcggtc aggcacagtt gccttatctt
    18301 agtttgctga aatccttctt tttcaaaatg gcttagtcca ctttctatta acttttggtg
    18361 agttataata taatctctgg tttctatggg tacttcctcc taataagatg aattaacttt
    18421 attatcttac acaggacttg aagtttcaat cgttaacgtt tattactgaa ctcattcctt
    18481 agagctaaac taatgagaaa gagcagaata actgatacaa aaagcaatcc tacaagaatt
    18541 aatagactca tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat
    18601 tatatccagc tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct
    18661 ccctaaaaat aaagtctttt acttaaaacc ttttttgtaa tcgtcaataa attttttccc
    18721 tttatcagtt aattgaaatc ctatatctaa atagtcctca tatctaagtt tagccagcat
    18781 ctcacttaca gatgctggac taacacttag aacttttgct aaatcattat tttttaattt
    18841 cttttttctt tttgataatt cctgaattgc tagtaaataa attagtatag tagtcgtttt
    18901 aatttgtacc atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg
    18961 ttccatttca tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca
    19021 gtaaaaatcg ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta
    19081 tcatccacac tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact
    19141 aagtgtttta tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca
    19201 gaaggcacat tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt
    19261 tttttatcat tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat
    19321 ttttcaatat acttatctag attttcttta tagaattttt tattatcagg atctttttcc
    19381 gctaactgtt tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca
    19441 tgaggatctt ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca
    19501 cttgctgcaa aatagtcttt gttttcctct ttgttcgcat ttttaactaa ttttgtaaac
    19561 caagcatttc ctccagtttc caagttaaca ccgttataaa aaatcaaatc tgcttttgaa
    19621 gttttttgaa catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg
    19681 atactgtgta aatcaatttt atcttttgct atattttcgg taatatctgc taaaatcgaa
    19741 ttagtaacta ctacttttaa tttttcgttc gaagccactt tatctttgtc tttactattt
    19801 attgaacatg ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct
    19861 ttccattttc tcatcataac gatctctcct tctttcttaa aaatttttgt tttggtgaga
    19921 ttagaaaact aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta
    19981 tgttaaaact ataaccaata aacaatccaa gcaatgatga acttgctccg actgttgcag
    20041 aaataaatat catagttttc aagctatttg catataaata tgctgtagct gctggtgtaa
    20101 ttaacattgc aacaattaat atagttccca cactttgcat agctgttact gctactaaag
    20161 ttaatagaac catcaataga taatgataaa aactgacatt catccctatt gcttttgcca
    20221 ttaatggatc aaaagaagtg attaataatt ctttaaaaaa tagaactact ataaccaaca
    20281 ctaaaaccga aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc
    20341 caaataaaat atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta
    20401 gtgctaaaaa agaactgaaa gtaataccaa ttgctgtatc tccttttatt acactattgc
    20461 tttttataaa agtaataatg atagaagcta atagaccaaa tacaatcgca ccaataaaaa
    20521 aattaatacc taaaatataa gaaattgcta ctcctggcaa aacagcatga gaaatagcat
    20581 cacccataag agacattcct cttaaaataa taaaacaacc tactgttcca gaaacaatcc
    20641 ctataactac agatgtaatt agagcattct gtaaaaagtg aaagtcatac agaccattaa
    20701 taaaattttg aagcatatta ttccccctct ccaataaata tagtatctcc ataggctttt
    20761 tttaaatttt cttcattaaa tacagattct acacttccgt gagcaattag atttcgattc
    20821 aaaagaataa tattatcaaa atacttcttt accttgctta aatcatgatg cacaattagc
    20881 actgtttttc cctgatgttt caattttttt aacgtatcca taattattcg ttcactaact
    20941 aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg agcaagacat
    21001 cttgctaaaa gtacacgttg aaattgtcct ccagataatt ctcctatttg atgattagaa
    21061 tattttccca tatcgacctt ttccaaagcc tttgaaactc tttgccattc tgcattttta
    21121 attctttgaa aaactttcat tccggcatat gttcccatag atacacattc ttttactttt
    21181 attggaaaag taaaatcaat atcagctttt tgctctacat atgcaatctt caccagtttt
    21241 ttattgatat cttcattatt aatggagacc tctccctgat gagggataat atttaacatt
    21301 gctttaagca atgtggattt tccagcacca tttggaccaa taatacctgt taaagcacct
    21361 tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca aacattaagc
    21421 tttttcactt ctaacataac tttctccttc taaatgataa tgattatcac gatcatatgg
    21481 tatacaaaaa ttatatttta gtcaacccta aattttatag tttagatttt tttctgttgc
    21541 tgaagattag aatgacaaaa ctatttgaaa ctacgcgtat agtaagtagc tttcaaaata
    21601 tataaaaaag gagcgttctc aaatgagaac atgctcctaa aactacttta ttggcgtatg
    21661 gaaaaattat acttgaagtg gatagcgggt tgaataagtt gataagcatc aaaacattta
    21721 taaaaattat tcaagactat gtctgttcca tatacaaata tgaaaatcag taaactccat
    21781 ttaacttttt tcgtagtgtg tctttttata catgtttatc gttattaata gtgaatagga
    21841 aaaaaactat ttttgatcat tgacaattaa atacaatttt tgaataacga tccctgctat
    21901 tcgagctaga aactcaagta cgccatgacc tgattagcga caaatactta gagatttctt
    21961 tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa ataatgatac ttccgtaagg
    22021 ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt acccaaagca attcaaaaat
    22081 ttcataacta ctattttagg agatgatttt atagaatcca gagtctacct gaataaaaaa
    22141 caaacctgtg catttctagg aatcgataat aatactctag ataagtggat tcgtatatat
    22201 aatttcccag taataagagt tggtggtgta gttcgctttg acgtagaaga tattaaagac
    22261 tttatgaata catttaaaat aacttgaaat tttcttctcg ccgcatatac taaaattagt
    22321 gaaatatact gccttacttt ttttaaggag tgaggatttt gcctacaaag ctttcaaatg
    22381 ggaaatataa aactaatctg agatatccaa aaaggtttaa agaaataaca ggaattgcat
    22441 cagaaaagta tcaaaaaaca tttccaaata ggcaattggc gattaaagcc gaaaatgata
    22501 tgaagaagaa aatagaaaaa gttttgagag aggagaatgc aaactcatta gaattaaaag
    22561 gaaaaataac gtttaaaaaa ttttacgaat ctaaatggct tcctagatat gaattaggtc
    22621 aaacaattag atcaaacagg cctcctagtg atataaccat tagtaataca aaagatatat
    22681 ttcggttgca tattttacct atgtttggtg aatacgcaat gaattatcta aatatcaata
    22741 ctgagataat atcagatgaa ttaaccaaaa aatcgaaaga atatgcaaat ataaaaatca
    22801 taaaaggata tgttcgttcc atgttcgata ttgcagaaat actaaattat atcgaattta
    22861 atcgaactac taaaattatt caaagtatca ctgcacctaa aaaaaatgct ttagaagaaa
    22921 aaagaataca agaaggtaag caagcactat catcaaaaga acttactaat tggatagaag
    22981 cagtaaatga tgattttaat aatcatttac ttacttttca tgattacaca ctttttatga
    23041 ttaccctgta tcttggtgat agaaaaagtg agacttacgc tttgcaatgg aaatatattg
    23101 attttgaaaa acaaacggtc cgtcttaaac atactttaga taaatatcaa agaaaaaagt
    23161 ttacaaaagg tcgaaaagat accgtcattc aagtaccaga agttgtaatg acattgctaa
    23221 gtgaatggaa atcggtacaa gctgatcaat tgttgaaatt aaaaattaag caaacgttag
    23281 atcagtatct ctttacttat actaaaccat ctggagaagt aaattgtcct gttcatgccg
    23341 attacctaaa ctatcgaatc aattcaatta aaagaaggca tcctgactta gttcatttat
    23401 ctccacacaa actccgtcat acttatgcaa ctatagctag acaaggtgga gcagatatga
    23461 atcaaatttc aaatgcttta acacactcag atatttctac aacaaagata tatgtgaaca
    23521 caccagatat agttgataag gctgtttttg aagcttttca gagaggattg aataagtgtg
    23581 attgatatgt gttcttaaat gtgttcttaa aaacaaaaga agcactttcc aaataattga
    23641 aaagtgccag aaacgttgat atataaagga tattaacgtt ttgagaattg tgaagcttta
    23701 cgtgctttct taagacctgg ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag
    23761 ccagcacgtt taagagctaa acggaagtca ggatcaactt ctagcaatgc acgagcgata
    23821 ccatgacgga tagctcctga ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg
    23881 tcatatgcgc cttttgtttc agtaacagcg aaaggttgat tgattacttc acgcaagtca
    23941 gcatgtggaa tgtattcttc aacatctttt ttatttacag taattttgcc agttcctggt
    24001 actagacgta cgcgagcaac tgcgttttta cgacggcctg tgccgatata ttgaacttgt
    24061 gccaatgaaa ttccctccta ttagattaag tttgtgatgt ctaaaacttc tggttgttgt
    24121 gctgcatgtg gatgttctgc tccaccgtat acgttcaatt ttgtaaattg tttgcggcct
    24181 aaagtgtttt taggaagcat accttttaca gaagtttcga tcaaacgacg agaattttta
    24241 gcacgcaatt cgccagcagt gattgatttc aatcctcctg ggtagttgct gtgacggtaa
    24301 taaattttgt cagtcgcttt tttacctgtt aatttcactt tgtctgcatt gatgacgatg
    24361 acgaaatcgc cagtatccaa atgaggtgtg aaagttggtt tatttttacc acgtagtaca
    24421 gatgcaacaa ctgttgaaag acgtcccaat ggaacatcag ttgcgtctac tacataccat
    24481 ttacgttcta cttcgcctgg tttggccata tatgttgtac gcacgttatt ttcctccaat
    24541 ttaagttctg atgtttgaca tacacttcga gtttccgggg ctctttgtgg ggcaaacaat
    24601 accatttaac atattacccg ctaatctagt caatgtcaac gtttttctgc ttgattgaaa
    24661 aatttatatg aaactttact tcttttcttt cttttatata aaagggtcgt ttcctgtcaa
    24721 aatgaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa
    24781 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccataaaac ataagaagct
    24841 gttttttaaa attgtttctt gtgactcgga gctgaacggc aggcttacaa cctcttggta
    24901 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
    24961 tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttt
    25021 cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt
    25081 ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg
    25141 gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac
    25201 gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt
    25261 catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta
    25321 atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat
    25381 ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa
    25441 caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt
    25501 ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc
    25561 aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg
    25621 taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata
    25681 agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt
    25741 atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact
    25801 gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc
    25861 ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa
    25921 aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca
    25981 aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat
    26041 ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca
    26101 aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg
    26161 cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga
    26221 ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct
    26281 agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc
    26341 tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa
    26401 agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc
    26461 tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag
    26521 acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa
    26581 gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga
    26641 agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat agtctttgat
    26701 accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat
    26761 tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc
    26821 aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat
    26881 cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca tcgcagagga
    26941 gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac
    27001 ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg
    27061 gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac
    27121 tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca
    27181 aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg
    27241 agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac
    27301 cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg
    27361 ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt
    27421 tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg
    27481 tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat
    27541 atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc
    27601 tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt
    27661 ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca
    27721 atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct
    27781 agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt
    27841 ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag
    27901 tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc
    27961 acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag
    28021 gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat
    28081 gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt
    28141 cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt
    28201 gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc
    28261 gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc
    28321 ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg
    28381 ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca
    28441 atagatttcc accaactgat cataagaaat aacttctgga tcatattcga tctctactgc
    28501 ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc
    28561 gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat
    28621 acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt
    28681 ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga
    28741 aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa
    28801 acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc
    28861 aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc
    28921 aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca
    28981 ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc
    29041 gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt
    29101 gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt
    29161 ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt
    29221 tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg
    29281 gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct
    29341 aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg
    29401 aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag
    29461 atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta
    29521 aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt
    29581 cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc
    29641 tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta
    29701 cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt
    29761 cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac
    29821 cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca
    29881 tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa
    29941 aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg
    30001 ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt
    30061 ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg
    30121 ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa
    30181 caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg
    30241 gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg
    30301 cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga
    30361 ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc
    30421 catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt
    30481 gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca
    30541 gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc
    30601 tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt
    30661 tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca
    30721 tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa
    30781 accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga
    30841 tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca
    30901 ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc
    30961 gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata
    31021 ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt
    31081 tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt
    31141 ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt
    31201 gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg
    31261 atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata
    31321 tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa
    31381 atgatcatcg acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt
    31441 ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc
    31501 ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt
    31561 tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct
    31621 ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc
    31681 taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa
    31741 gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt
    31801 tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg
    31861 gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg
    31921 atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact
    31981 aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt
    32041 agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa
    32101 ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc
    32161 aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc
    32221 aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc
    32281 gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag
    32341 ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt
    32401 tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc
    32461 ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat
    32521 ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg
    32581 gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct
    32641 tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata
    32701 ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc
    32761 gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc
    32821 atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg
    32881 tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt
    32941 tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct
    33001 tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc
    33061 agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc
    33121 cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta
    33181 ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc
    33241 ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga
    33301 agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt
    33361 gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg
    33421 gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc
    33481 actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta
    33541 cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca
    33601 tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca
    33661 gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct
    33721 acttgtccag cgcctatgat tgctactttc ttcatttatt tctctctcct ttgcttattt
    33781 acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca
    33841 gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca
    33901 aacgacattc atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc
    33961 gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat
    34021 gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca
    34081 tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac
    34141 gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa
    34201 taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt
    34261 tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc
    34321 cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct
    34381 cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat
    34441 cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg
    34501 tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt
    34561 tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga
    34621 atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg
    34681 gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat
    34741 ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt
    34801 acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt
    34861 tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa
    34921 ggcaacataa gaaatgatcc cgccatagcc gatccctatt aaagaagctt gtaatgcggc
    34981 agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag
    35041 acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt gtttttctgc
    35101 taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt
    35161 agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc
    35221 tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac
    35281 tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt
    35341 tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc
    35401 tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc
    35461 gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc
    35521 tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg
    35581 ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc
    35641 gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt
    35701 cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc
    35761 gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca
    35821 ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa
    35881 atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa
    35941 agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa
    36001 tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg
    36061 tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga
    36121 taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa
    36181 caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc
    36241 gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag
    36301 ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa
    36361 tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt
    36421 gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg
    36481 gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct
    36541 ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat
    36601 gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt
    36661 ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa
    36721 catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc
    36781 cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc
    36841 ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata
    36901 gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca
    36961 cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata
    37021 actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact
    37081 taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc
    37141 tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa
    37201 acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat
    37261 aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga
    37321 aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat
    37381 ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc
    37441 tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt
    37501 tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac
    37561 tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc
    37621 gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc
    37681 aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt gtttattcac
    37741 tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa
    37801 gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt
    37861 caatccagat actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat
    37921 attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat
    37981 tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat
    38041 cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat
    38101 cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa
    38161 cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc
    38221 tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa
    38281 accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg
    38341 tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata
    38401 attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc
    38461 tttatccatc actttagcaa aatcctgact gacttccatt acttctggta atttaccgga
    38521 acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat
    38581 tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg
    38641 ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct
    38701 ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat
    38761 aatttttttc atttttttcc acctcttatt ttattatttt cagccacata tattcatacg
    38821 gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta
    38881 ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta
    38941 agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta
    39001 agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg
    39061 tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg
    39121 ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt
    39181 aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta
    39241 ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag
    39301 aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt
    39361 ttttcttgta gtttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg
    39421 ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa
    39481 aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact
    39541 tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga
    39601 ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat
    39661 ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt
    39721 ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa
    39781 atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat
    39841 cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa
    39901 aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc
    39961 tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat
    40021 ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat
    40081 gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc
    40141 atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg
    40201 tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat
    40261 aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag
    40321 taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct
    40381 gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc
    40441 gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt
    40501 cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat
    40561 tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta
    40621 aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa
    40681 aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata
    40741 taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg
    40801 tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc
    40861 aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg
    40921 gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt
    40981 ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga
    41041 ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag
    41101 aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg
    41161 aattggatca atataatatt cctattgtgg taatcggaaa agtcggaaac gactacacga
    41221 acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga
    41281 tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca
    41341 atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg
    41401 aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa
    41461 tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta
    41521 tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat
    41581 tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga
    41641 caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc
    41701 ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac
    41761 aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg
    41821 ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt
    41881 attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt
    41941 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
    42001 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
    42061 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
    42121 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
    42181 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
    42241 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
    42301 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
    42361 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
    42421 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
    42481 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
    42541 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
    42601 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
    42661 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
    42721 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
    42781 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
    42841 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
    42901 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
    42961 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
    43021 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
    43081 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
    43141 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
    43201 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
    43261 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
    43321 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
    43381 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
    43441 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
    43501 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
    43561 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
    43621 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
    43681 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
    43741 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
    43801 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
    43861 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
    43921 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
    43981 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
    44041 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
    44101 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
    44161 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
    44221 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
    44281 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
    44341 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
    44401 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
    44461 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
    44521 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
    44581 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
    44641 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
    44701 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
    44761 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
    44821 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
    44881 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
    44941 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
    45001 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
    45061 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
    45121 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
    45181 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
    45241 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
    45301 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
    45361 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
    45421 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
    45481 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
    45541 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
    45601 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
    45661 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
    45721 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
    45781 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
    45841 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc acgttcaggt
    45901 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
    45961 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
    46021 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
    46081 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
    46141 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
    46201 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
    46261 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
    46321 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
    46381 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
    46441 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
    46501 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
    46561 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
    46621 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
    46681 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
    46741 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
    46801 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
    46861 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
    46921 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
    46981 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
    47041 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
    47101 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
    47161 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
    47221 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
    47281 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
    47341 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
    47401 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
    47461 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
    47521 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
    47581 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
    47641 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
    47701 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
    47761 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
    47821 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
    47881 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
    47941 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
    48001 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
    48061 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
    48121 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
    48181 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
    48241 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
    48301 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
    48361 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
    48421 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
    48481 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
    48541 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
    48601 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
    48661 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
    48721 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
    48781 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
    48841 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
    48901 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
    48961 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
    49021 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
    49081 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
    49141 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
    49201 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
    49261 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
    49321 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
    49381 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
    49441 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
    49501 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
    49561 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
    49621 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
    49681 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
    49741 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
    49801 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
    49861 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
    49921 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
    49981 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
    50041 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
    50101 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
    50161 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
    50221 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
    50281 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
    50341 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
    50401 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
    50461 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
    50521 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
    50581 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
    50641 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
    50701 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
    50761 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
    50821 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
    50881 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
    50941 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
    51001 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
    51061 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
    51121 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
    51181 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
    51241 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
    51301 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
    51361 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
    51421 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
    51481 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
    51541 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
    51601 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
    51661 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
    51721 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
    51781 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
    51841 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
    51901 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    51961 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    52021 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    52081 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    52141 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    52201 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    52261 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    52321 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    52381 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    52441 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    52501 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    52561 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    52621 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    52681 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    52741 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    52801 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    52861 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    52921 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    52981 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    53041 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    53101 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    53161 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    53221 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    53281 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    53341 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    53401 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    53461 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    53521 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    53581 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    53641 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    53701 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    53761 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    53821 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    53881 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    53941 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    54001 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    54061 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    54121 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    54181 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    54241 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    54301 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    54361 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    54421 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    54481 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    54541 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    54601 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    54661 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    54721 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    54781 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    54841 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    54901 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    54961 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    55021 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    55081 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    55141 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    55201 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    55261 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    55321 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    55381 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    55441 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    55501 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    55561 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    55621 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    55681 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    55741 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    55801 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    55861 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    55921 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    55981 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    56041 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    56101 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    56161 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    56221 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    56281 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    56341 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    56401 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atca
//
LOCUS       DACDTO010000009        55573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDTO010000009 DACDTO010000000
VERSION     DACDTO010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.009"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            271..591
                     /locus_tag="IWK70_000588"
     CDS             271..591
                     /locus_tag="IWK70_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9613160.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            745..1650
                     /locus_tag="IWK70_000589"
     CDS             745..1650
                     /locus_tag="IWK70_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9613161.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1647..2420
                     /locus_tag="IWK70_000590"
     CDS             1647..2420
                     /locus_tag="IWK70_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9613162.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2601..4025)
                     /locus_tag="IWK70_000591"
     CDS             complement(2601..4025)
                     /locus_tag="IWK70_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9613163.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4236..4682
                     /gene="nrdI"
                     /locus_tag="IWK70_000592"
     CDS             4236..4682
                     /gene="nrdI"
                     /locus_tag="IWK70_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9613164.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4747..6729)
                     /locus_tag="IWK70_000593"
     CDS             complement(4747..6729)
                     /locus_tag="IWK70_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9613165.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="IWK70_000594"
     CDS             complement(6743..7639)
                     /gene="murQ"
                     /locus_tag="IWK70_000594"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9613166.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7642..8703)
                     /locus_tag="IWK70_000595"
     CDS             complement(7642..8703)
                     /locus_tag="IWK70_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9613167.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(8914..9441)
                     /locus_tag="IWK70_000596"
     CDS             complement(8914..9441)
                     /locus_tag="IWK70_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9613168.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(9508..11010)
                     /locus_tag="IWK70_000597"
     CDS             complement(9508..11010)
                     /locus_tag="IWK70_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9613169.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11268..12389)
                     /locus_tag="IWK70_000598"
     CDS             complement(11268..12389)
                     /locus_tag="IWK70_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9613170.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12515..13456
                     /locus_tag="IWK70_000599"
     CDS             12515..13456
                     /locus_tag="IWK70_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9613171.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13606..14502)
                     /locus_tag="IWK70_000600"
     CDS             complement(13606..14502)
                     /locus_tag="IWK70_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9613172.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14771..16111
                     /locus_tag="IWK70_000601"
     CDS             14771..16111
                     /locus_tag="IWK70_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9613173.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16329..16772
                     /locus_tag="IWK70_000602"
     CDS             16329..16772
                     /locus_tag="IWK70_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9613174.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="IWK70_000603"
     CDS             complement(17276..18694)
                     /gene="nhaC"
                     /locus_tag="IWK70_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9613175.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19097..20320)
                     /locus_tag="IWK70_000604"
     CDS             complement(19097..20320)
                     /locus_tag="IWK70_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9613176.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20351..21190)
                     /locus_tag="IWK70_000605"
     CDS             complement(20351..21190)
                     /locus_tag="IWK70_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9613177.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21207..21887)
                     /locus_tag="IWK70_000606"
     CDS             complement(21207..21887)
                     /locus_tag="IWK70_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613178.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTIYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21889..22962)
                     /locus_tag="IWK70_000607"
     CDS             complement(21889..22962)
                     /locus_tag="IWK70_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9613179.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(23609..24280)
                     /locus_tag="IWK70_000608"
     CDS             complement(23609..24280)
                     /locus_tag="IWK70_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9613180.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24367..27045)
                     /gene="mgtA"
                     /locus_tag="IWK70_000609"
     CDS             complement(24367..27045)
                     /gene="mgtA"
                     /locus_tag="IWK70_000609"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9613181.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27704..28141
                     /gene="ndk"
                     /locus_tag="IWK70_000610"
     CDS             27704..28141
                     /gene="ndk"
                     /locus_tag="IWK70_000610"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9613182.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28163..29029)
                     /locus_tag="IWK70_000611"
     CDS             complement(28163..29029)
                     /locus_tag="IWK70_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9613183.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29189..31282)
                     /locus_tag="IWK70_000612"
     CDS             complement(29189..31282)
                     /locus_tag="IWK70_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9613184.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32031..32654)
                     /locus_tag="IWK70_000613"
     CDS             complement(32031..32654)
                     /locus_tag="IWK70_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613185.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32813..34210)
                     /locus_tag="IWK70_000614"
     CDS             complement(32813..34210)
                     /locus_tag="IWK70_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9613186.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34242..35213)
                     /locus_tag="IWK70_000615"
     CDS             complement(34242..35213)
                     /locus_tag="IWK70_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9613187.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35587..36012
                     /locus_tag="IWK70_000616"
     CDS             35587..36012
                     /locus_tag="IWK70_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613188.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36500..37201)
                     /locus_tag="IWK70_000617"
     CDS             complement(36500..37201)
                     /locus_tag="IWK70_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9613189.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37217..37657)
                     /locus_tag="IWK70_000618"
     CDS             complement(37217..37657)
                     /locus_tag="IWK70_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9613190.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37657..38232)
                     /locus_tag="IWK70_000619"
     CDS             complement(37657..38232)
                     /locus_tag="IWK70_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9613191.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39080..39559)
                     /locus_tag="IWK70_000620"
                     /pseudo
     CDS             complement(39080..39559)
                     /locus_tag="IWK70_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39718..40419)
                     /locus_tag="IWK70_000621"
     CDS             complement(39718..40419)
                     /locus_tag="IWK70_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9613192.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40527..41936)
                     /locus_tag="IWK70_000622"
     CDS             complement(40527..41936)
                     /locus_tag="IWK70_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9613193.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(41984..43849)
                     /locus_tag="IWK70_000623"
     CDS             complement(41984..43849)
                     /locus_tag="IWK70_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9613194.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(43970..44821)
                     /locus_tag="IWK70_000624"
     CDS             complement(43970..44821)
                     /locus_tag="IWK70_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9613195.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45131..45844)
                     /locus_tag="IWK70_000625"
     CDS             complement(45131..45844)
                     /locus_tag="IWK70_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9613196.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(45912..46238)
                     /locus_tag="IWK70_000626"
     CDS             complement(45912..46238)
                     /locus_tag="IWK70_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9613197.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46361..46588)
                     /locus_tag="IWK70_000627"
     CDS             complement(46361..46588)
                     /locus_tag="IWK70_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9613198.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(46910..48163)
                     /locus_tag="IWK70_000628"
     CDS             complement(46910..48163)
                     /locus_tag="IWK70_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9613199.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48224..48964
                     /locus_tag="IWK70_000629"
     CDS             48224..48964
                     /locus_tag="IWK70_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9613200.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49050..49808)
                     /locus_tag="IWK70_000630"
     CDS             complement(49050..49808)
                     /locus_tag="IWK70_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9613201.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49805..50833)
                     /locus_tag="IWK70_000631"
                     /pseudo
     CDS             complement(49805..50833)
                     /locus_tag="IWK70_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(50853..54020)
                     /locus_tag="IWK70_000632"
     CDS             complement(50853..54020)
                     /locus_tag="IWK70_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613202.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54244..55128)
                     /locus_tag="IWK70_000633"
     CDS             complement(54244..55128)
                     /locus_tag="IWK70_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9613203.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55223..55570)
                     /locus_tag="IWK70_000634"
     CDS             complement(55223..55570)
                     /locus_tag="IWK70_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613204.1"
                     /translation="MDHLYIKYIHQFSVLVLGTLIIAGIVAFIIQGRDILTVHQEFFS
                     YAFGIPLIAIRSDNKKWKKRYYILLLFLCFYLPPKIFTLLGIQCLSFSFISYIIVLIF
                     LSFIVWMFYLVKK"
BASE COUNT        15958 a        11708 c         9579 g        18328 t
ORIGIN      
        1 tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag cattcatctt
       61 cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa gtatgagatt
      121 gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg ccaccgggta
      181 ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct ctttttgtgt
      241 atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat catagcagga
      301 attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc taaaatcaat
      361 tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc aaaggcaatc
      421 aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc agttggttcg
      481 gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg gttctttgtg
      541 gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta gacacttgga
      601 ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt ttatgaaaaa
      661 ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat gcttgtacta
      721 tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt atccaagaac
      781 tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg caaaatcgtt
      841 ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat tttaggactg
      901 atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc ctctagtcat
      961 aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc attcttaaca
     1021 ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat cgacactatc
     1081 gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta ctcgttggga
     1141 atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat cgttatttta
     1201 gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa aatcattacc
     1261 gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag cgagcttgaa
     1321 aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca atgtacaatg
     1381 gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa tgatctagct
     1441 gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa aatcaagctt
     1501 ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc acaaaatatc
     1561 catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt ggaaattctg
     1621 gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa ttatttaaat
     1681 tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg ctcataggaa
     1741 cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg actgctcagc
     1801 tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc acgatcattt
     1861 cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat tctcgaggag
     1921 aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta taccttttag
     1981 ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc ttcacggaga
     2041 aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg ggtagttata
     2101 tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc aatagttccg
     2161 gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata tctggtatcc
     2221 ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt atgttgaatc
     2281 tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt tctactaatc
     2341 aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga tatctcgtat
     2401 ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag acgttcagcc
     2461 ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc tgtgacaaca
     2521 tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc acaacctctc
     2581 tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa aaccattgca
     2641 atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat atgttgtaca
     2701 ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa gaaaagaaca
     2761 agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg aaaggcactt
     2821 gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag tttcatttgg
     2881 tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt gtaagcaaag
     2941 ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca taacgtcgtc
     3001 aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc tttaggcaaa
     3061 agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca tgagagccaa
     3121 acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca tttaccaacc
     3181 atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag ctgattcatc
     3241 gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa gccgaatatc
     3301 cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc tacgacagag
     3361 gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa tacttgactg
     3421 gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa gttatcaatg
     3481 aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa tggcactaat
     3541 ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg attcactagc
     3601 catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt ttgcccacct
     3661 tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa agctacgttg
     3721 cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa acggccaaaa
     3781 ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt attgatatta
     3841 ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact gaaaatagct
     3901 gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc tccaatcgct
     3961 gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg ttgctctgta
     4021 tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac aaaccttttt
     4081 tatttttact taattaataa aaactttaat agaaatataa ttctaccggc ttttttctca
     4141 ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac ttaaaaaaga
     4201 tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat atatcgattt
     4261 caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa atgcatgcag
     4321 aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt gattttacga
     4381 aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga aatggtttag
     4441 ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa catgaagaca
     4501 atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat aatcagtatt
     4561 gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca gattatgaat
     4621 tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa aacaataaat
     4681 aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc tattgtttct
     4741 atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac ctgttttttc
     4801 caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg atgtttcttt
     4861 gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag gtgtcacttg
     4921 ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg tgttgattcc
     4981 catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt gtttcgttgg
     5041 gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt ctggttctac
     5101 aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt tatctaaaga
     5161 aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg aagtttgagg
     5221 tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt cttctttttc
     5281 taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag cataagctgc
     5341 caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga tcaaagcggc
     5401 tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca ctgccccgcc
     5461 gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg ttactccgta
     5521 aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga tcatttctgt
     5581 taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca cttgtcctgc
     5641 accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa tcatttgcac
     5701 atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga ataatgtgcc
     5761 tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc caccgaccat
     5821 gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca gaccgatggc
     5881 aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta ctttatgcag
     5941 ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga tcccaccttg
     6001 tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg gattcatccc
     6061 tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac caaaaacttg
     6121 tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt tcaagatgtt
     6181 catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg tgactaagtt
     6241 tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag gaatcattgg
     6301 cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt tttgtttcga
     6361 tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc cagatgctga
     6421 tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca cgcctgccaa
     6481 atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga cttgaagctg
     6541 ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga catttacaag
     6601 atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc tttctacatt
     6661 gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag tcagttcgtt
     6721 ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt gcttcagcta
     6781 attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata gccagcttaa
     6841 cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct tctgtcgctt
     6901 gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg acatcgacca
     6961 tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc atattcaaga
     7021 taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat tctggacctg
     7081 catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta ttgcaagaaa
     7141 tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc acatatggtg
     7201 tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc agcttgatta
     7261 gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca gtcattgctt
     7321 cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat gtcggtacgc
     7381 attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata taaatcagac
     7441 gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata cttgtcagtt
     7501 ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga acggcttgag
     7561 aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt gttaggttct
     7621 ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat agttcatacc
     7681 agctttgatt tggttgatca atggtagctc ttctttgata actttagcta ctacattcac
     7741 tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat agcgcaaata
     7801 cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc ttgctgggat
     7861 aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg catcgtcttg
     7921 ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag gttccgtatg
     7981 gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt tttctgacag
     8041 acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta gagcgcctgc
     8101 aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata gtgggcctct
     8161 caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt tgagccattg
     8221 attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat aataattatg
     8281 ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga tatctttagg
     8341 cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg caatctgctg
     8401 attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca aaggcgttag
     8461 atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga ccatcaattc
     8521 tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat atgccgaaac
     8581 atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg atgccatttg
     8641 gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg aaaagccaaa
     8701 catattatcc ctctttttct gtatccgtct tcatttctat gactagagta tatatctaaa
     8761 gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa ggttgagttt
     8821 atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa gcagtgcctt
     8881 gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca attctgtatt
     8941 atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag cattgatcac
     9001 tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt cgatgcattc
     9061 tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat ttgggaatcg
     9121 tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca gtccatcata
     9181 atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc tgagacgatc
     9241 agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga ttttcgtgat
     9301 tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct gtcctttttc
     9361 agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag ttgtttctcc
     9421 gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc ccttaaccat
     9481 accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa tctcagtaga
     9541 tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt tcacgttcaa
     9601 gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg gttcatccca
     9661 tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt tccgttggcc
     9721 catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa gctttcggag
     9781 gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg ccaacgttcc
     9841 tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg gctggtattt
     9901 ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg atgactttcc
     9961 tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa aatgaattgg
    10021 ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa gtcgcttatg
    10081 gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt ttaatagttt
    10141 ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc gtttcatcgt
    10201 ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag tatcgatgaa
    10261 tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg ctttttcagc
    10321 tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag ccatttcaaa
    10381 ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat aaagttccag
    10441 ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc cccggtcatg
    10501 gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt gttttctacc
    10561 agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt catccacaaa
    10621 taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga attctctcca
    10681 taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc atatctcaaa
    10741 atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg tctgttgcgg
    10801 gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt tgttttgtag
    10861 aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta gtttccattg
    10921 tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt cgtagccgaa
    10981 tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa aagaccagtc
    11041 ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa agacccatac
    11101 atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct ccacaaggta
    11161 cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat ccctcacttt
    11221 gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta cttcgggagg
    11281 atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa tgtttgcctg
    11341 tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac aattgggaga
    11401 aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat tattggtttt
    11461 cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat cgtttgctga
    11521 tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt ttccaatcca
    11581 gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa aaaaagggct
    11641 ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac ctcccagaaa
    11701 tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag cgtcgtatct
    11761 attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt atatggattg
    11821 gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct tcgatcaaaa
    11881 tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa agaagcaccc
    11941 aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa agtgacaata
    12001 atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc ataactggaa
    12061 gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa tgcttgtgca
    12121 gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct gatttttcgc
    12181 tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag cgcgtaatag
    12241 attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt tcttccttta
    12301 cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc acttccttta
    12361 aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct taagtaagac
    12421 actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt ctatatacta
    12481 aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag ctggggccgg
    12541 agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg tcctcttgat
    12601 cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg ctgactttaa
    12661 cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca tcgaaagcaa
    12721 cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg atatgttttg
    12781 ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa atggattagg
    12841 tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg ggatcacgat
    12901 gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg gagaaatcga
    12961 actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag aagtctttca
    13021 agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt ggcgaaaagc
    13081 ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata ttgctgaact
    13141 aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat ttgcttctat
    13201 cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata ttgcttctac
    13261 ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc tgatcaaaaa
    13321 ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag gacaaaaata
    13381 tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa aagaaggaat
    13441 ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag ctggttctct
    13501 ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc cataagaatt
    13561 gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt tgttttcaac
    13621 agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga taaatcgttt
    13681 tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct taaatggtgt
    13741 atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa tattttcagg
    13801 acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct tttgaaaata
    13861 catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca gttccagaat
    13921 cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc caggttgttc
    13981 attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta taaattcctg
    14041 cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa atagatccgt
    14101 ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat tatgtttcag
    14161 cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg ccggttcatc
    14221 ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa aaaataacac
    14281 aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa atccataatc
    14341 taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa tgaattccga
    14401 ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca aaaaataaag
    14461 tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg cctcactttc
    14521 tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa aacgtttcct
    14581 atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc attcttctat
    14641 taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg ttatcattag
    14701 tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat ttaaaagaaa
    14761 gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac atatcaccat
    14821 gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg aagcgattgg
    14881 gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt tatatatcgt
    14941 gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg gattatctgg
    15001 tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg tctactggtc
    15061 tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt tgcagttgtg
    15121 gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa ctacgatcat
    15181 caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt ttgctaaaat
    15241 cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc aaatgttggg
    15301 cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta ctcctcatgg
    15361 tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg gttctgaact
    15421 gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta aagcaatcaa
    15481 aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc ttttgattct
    15541 ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg tatttgaaaa
    15601 aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg ctttgttctc
    15661 atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt tgaaggatcg
    15721 aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc aacgtgctgt
    15781 tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg tcggtgacaa
    15841 attatttggc tatctggcag gttccctttc gtatacagta ttattgatct ggatcattat
    15901 cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata aaggaatcag
    15961 cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca cgaactccat
    16021 tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt atcaaaaaaa
    16081 gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat tttatttatg
    16141 tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg caactccttt
    16201 ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc tctgtgcaaa
    16261 ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa aaaaggagtt
    16321 tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca acggtagtta
    16381 tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc agtcgtacac
    16441 gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg ttgttcatgt
    16501 ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt cttcttgttt
    16561 ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca gcttggatga
    16621 aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct gatttaaaag
    16681 atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa gagatcgaca
    16741 aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata aaaaaagagg
    16801 atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga acgccgtgaa
    16861 taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa caaaaatagc
    16921 ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt gaacaccgtt
    16981 tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa atggaaaaat
    17041 agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat tgaacaccgt
    17101 ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat tttgtgaaat
    17161 ggctttccac atttttgtat aaatttggct taataccgag aaactggctt ttgaataccg
    17221 tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta tgtttttatt
    17281 gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt tttgagattc
    17341 cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg aaaaatacga
    17401 atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt ccggcaattc
    17461 cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta cctaatacag
    17521 ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc agaataacag
    17581 ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat gttgtaaaaa
    17641 tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt gagatcaagc
    17701 caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac acagtaggca
    17761 tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt ccagtatttg
    17821 aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt gggttttgaa
    17881 taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt gccggcacct
    17941 ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt gaaatagtaa
    18001 agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg gctttacttg
    18061 tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca gttgaccaca
    18121 tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct agatttgttg
    18181 attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata gctccaacaa
    18241 ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc ccaatcgttg
    18301 acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca acaaatacag
    18361 aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga gttggaatga
    18421 tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata aagattggga
    18481 tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc gggtatcttt
    18541 ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata ttaggtgaga
    18601 cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat acaagtgttg
    18661 cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt ttagttgttc
    18721 ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc gcgattcctc
    18781 ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc ggtaccacct
    18841 taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat ggactttcca
    18901 tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct gaacaaaaca
    18961 cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat aagatcattt
    19021 gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata actttcacac
    19081 acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct actatttctt
    19141 gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca agagctgtat
    19201 tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt ttagcagcag
    19261 ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc gtaggctgga
    19321 ttgttggtat gacttggctg acatttccta catcagaaga accgagactt gttctttgtt
    19381 ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag aataagtcat
    19441 caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca aactcaaaag
    19501 tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca tatactttat
    19561 ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa tctggaatga
    19621 cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct tttggcaatt
    19681 gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa gcatttactc
    19741 ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg tcgactggat
    19801 cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg cataacgcta
    19861 catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat ccgttctcgc
    19921 ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca tcaatgaatt
    19981 gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc ccgcatgcat
    20041 gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga ccgggtttat
    20101 tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt tctacccaaa
    20161 acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca tagttactta
    20221 cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa cgatattgga
    20281 ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc tcctatgcta
    20341 gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat ccttttgatg
    20401 ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt ttttgatacg
    20461 ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga cgtgcatggt
    20521 catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca tctacagcta
    20581 caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt tgggaagcat
    20641 ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca gtgggcgctt
    20701 gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga agagctctgc
    20761 cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata tcagctacat
    20821 ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg atcgatacta
    20881 aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg aaagaattca
    20941 aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta aatttcacgt
    21001 attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat acatctgtat
    21061 cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta tctgcttttc
    21121 cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca aaacgaataa
    21181 tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag ttgattactg
    21241 ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat cgttacatct
    21301 gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc tgcacctact
    21361 gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga gacccgaatg
    21421 ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg actggttccc
    21481 atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt ctcgatttga
    21541 cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt cgcaccaatc
    21601 gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat aatgaaaggg
    21661 atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg gttagctagt
    21721 aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat catcccgcca
    21781 atgaatgctg tccaaaaaga catatatatt gtttgcttcg tcgcttcgac aaattcatcc
    21841 ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt atcttcctcc
    21901 ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta tttttacacc
    21961 agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag ctaaagtaac
    22021 gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata aaatattcgc
    22081 tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct gatttccaat
    22141 ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag aatgggcaat
    22201 gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt tggtcaattc
    22261 ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc ttccatcttc
    22321 cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag tgatcaggac
    22381 gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct gaagggtcgt
    22441 tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt cattcgccaa
    22501 agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag gataagcaga
    22561 tgctttttct tccaaaccga caagttccag tagttcattc actttttgac gacgttcctg
    22621 tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag ttcggctttc
    22681 cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc gcaattcttt
    22741 gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg ttggtttctg
    22801 cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac cgacgattcc
    22861 ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta tctctttttc
    22921 tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc ctccttatct
    22981 aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt ttttgcgtgt
    23041 taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg atcatactct
    23101 agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg aattgctcga
    23161 tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc tctgtcaaag
    23221 gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa gtccttttac
    23281 taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt ttcggaagtt
    23341 tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta acggtcgaac
    23401 agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc agcttttctt
    23461 acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta ctttcctttt
    23521 caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg aaaagtcaag
    23581 tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt aacatttcaa
    23641 taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt tcttcgacgg
    23701 tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct gctcgtgaag
    23761 cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct tctaatcctg
    23821 ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac acttcgtctc
    23881 cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata tctgttgctc
    23941 ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt aagtctgcca
    24001 atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt tgataaaaat
    24061 attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct gctttcacta
    24121 aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt tcttttggga
    24181 tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt ttttctgtgt
    24241 caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta atcctttcca
    24301 aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa gaaccagcct
    24361 tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag ctgtcacaag
    24421 cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag gaagtggcat
    24481 caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa taattcctaa
    24541 tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta ctttcggtgt
    24601 acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc atcctgcatg
    24661 gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac ttccgcccac
    24721 tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa atatcgaact
    24781 tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt tttttggctc
    24841 gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa catcatagat
    24901 cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga acgtacttgc
    24961 aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga tatatttcat
    25021 gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg tcaaatcttt
    25081 ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat ctacagaaat
    25141 accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta aaaatccgac
    25201 agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag gattcaattt
    25261 tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca tttccgagat
    25321 tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca ctgaacgtgt
    25381 aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt tcagagcttt
    25441 caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac caatcaaaac
    25501 catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg tcttttgtgc
    25561 gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa gtacggcttc
    25621 ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt tagaaatgtt
    25681 cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg gatcagcaac
    25741 cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt tttgataatt
    25801 gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat ctaacaaatt
    25861 tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac gatcatcttc
    25921 atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca atgtccccgt
    25981 tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca aatttttgat
    26041 cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg taacgatcat
    26101 tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa aagcttctag
    26161 ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca atgacataaa
    26221 tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt gaattggttt
    26281 ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag aaacaatgat
    26341 tccttccgca cttccactga tgacattact tcccataaag acaagatttt cgtaacttgt
    26401 ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat agctttcccc
    26461 tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca tatccgctgg
    26521 tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt ccataggaat
    26581 ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg atttcagttt
    26641 ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc tgacgataac
    26701 catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg ctaatacata
    26761 ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg gcgtgatgta
    26821 tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg cattttcacc
    26881 gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg atgcatggaa
    26941 tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt tttgtgtatc
    27001 tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt ctcgctgctt
    27061 ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct gagaacagat
    27121 agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt catccatttg
    27181 ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt tcgaatagtt
    27241 atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg cggcaacaaa
    27301 aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa aaagcttctc
    27361 agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct gcttttagct
    27421 accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc tctcgacgtt
    27481 tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat tcggttttcc
    27541 ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt ttttcatact
    27601 ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc tcattttcca
    27661 cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa gaacacttgt
    27721 tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc aacgttttga
    27781 agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg aattagcaaa
    27841 agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg attacatgac
    27901 ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata tcgtccgtaa
    27961 gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag gagactacgc
    28021 gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg cggtaaaaga
    28081 gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag cggcgaagtg
    28141 atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc agttttttct
    28201 ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag aattcttggt
    28261 agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa taatctgcca
    28321 tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt ttcatatctt
    28381 cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa gtcaggttca
    28441 ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt cgcattcttt
    28501 ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa ggaaaatcag
    28561 ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat tcttcaatga
    28621 ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt ttttcgacca
    28681 tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc ctacggttat
    28741 tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc attcgtcttt
    28801 ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat tctgcaagtg
    28861 tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt ttcactgaat
    28921 ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat tgggactgtg
    28981 tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata atagttctcc
    29041 tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga aaaatacacg
    29101 aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat tagaatgttg
    29161 tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt tcaacgtctg
    29221 ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa aagacacttg
    29281 tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca tttttatagt
    29341 catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc aagagatttt
    29401 cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga tctttggaga
    29461 tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga ttattgtaag
    29521 cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga aaagcaacta
    29581 cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct ttatcatcga
    29641 ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat tcatgattga
    29701 ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc atctgtgcgt
    29761 tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa tctttattat
    29821 ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt tggtaaatac
    29881 cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa tgtgccgttc
    29941 gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg aaattcttgt
    30001 catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa aacggctgtt
    30061 gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat atcccataac
    30121 ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca taacctaagt
    30181 ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa acagcgcttg
    30241 tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg tctgacccaa
    30301 gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac atattttcag
    30361 catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg atcgttgctt
    30421 gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc ttcatattaa
    30481 tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct ttagcgatcc
    30541 caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa acttcatcaa
    30601 tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg attccgtcat
    30661 aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca aatgttcgct
    30721 gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa ataagccctg
    30781 aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg ataggtattt
    30841 tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc cagtagatga
    30901 catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac actttcgaaa
    30961 agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa ataatattca
    31021 agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac gctggctttg
    31081 gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt gggttgatcc
    31141 acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca tagtaggcaa
    31201 tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa cgattttggc
    31261 acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt ttcttatttt
    31321 gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt ttattctttc
    31381 taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag cgattaaact
    31441 aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa aagccagttt
    31501 ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct ccttatttct
    31561 tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg ccaactcctc
    31621 ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt gttccttgtg
    31681 gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat cagctgaccc
    31741 tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg gtgttcaaaa
    31801 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta ttttttgaaa
    31861 ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgatac acggtgttca
    31921 atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgaggaact attttgccgt
    31981 ttgtttctct aatactcagg tcaaaacgct gatttcacaa cctctgtttt tcattgtcca
    32041 taggattgat attgtccttg tgatgactca ttcgtaataa ttgaggcttg attttcttca
    32101 cttcccgtta tcgtttgtcc actgccagaa tctgaaggac ttgttcctgt cgactcgttt
    32161 ttttgagaag agacagttgt tttcttgact tgttcatttg tctttttcca ttccgctaaa
    32221 cgatcggcga ccactttttt gtctactttt tcagaataag cggtgatcac ttttccattt
    32281 ttcaaacggg ctactttgtc gccatcatga gtcaaaagca aaacggtcaa atgcttgtct
    32341 ttaaattgat attgtctgag agaaatcgca ctaagtacgt catcgatatt aataaattga
    32401 ttttcttctt ttccttttgg caaataaatg tccaattcgc ctttttcgta attggcagtt
    32461 attttatact ccgcccacca ataagttgag aattcttttt tgaaattatc tactgattct
    32521 ttgcttgcta cgattttttc tgtttctttc tgagcaagat ctgctgtcat ttttcctgaa
    32581 aatgctgcgg ataaaataag cagtaagaaa aaaccgaata accaaaagag aagaaacggt
    32641 ctttttttcc tcatgctttc ctccactcct atttaatttt gactattcta ttatatacaa
    32701 aaaatgaaat agaaagacaa aaaagttgaa aaagatcgta aataattgaa cttctttttc
    32761 acaaaatggt aagaatggag aatttcacgc atttgctctc aactattctt tcttaagcct
    32821 caaatccgtt tttatctgat acgtctttga accaatgacc cgattttttg attgttcttt
    32881 ttccatcttg tgctaagtct actgaaataa agccatatcg attcttatat gcattggtcc
    32941 acgaccagtt atccatacat gtccacatat gatagcctaa acaattggca ccttcttgaa
    33001 tggcttgatg gacatatttc agatgagatt ggacaaattc gatccgataa tcatcgtgga
    33061 tcatgccatc ttctttgata aatcgtgctt cgttttctac acccataccg ttttcagaaa
    33121 taaagcagtc gatatttcca taatgatctc gtacattgat caaaatatca tagattcctt
    33181 gttcatagat ctcccatccg cgataaggat tcattttacg accaggcatt tcataattgt
    33241 cgaaataatc ctcaggcatt ggaccgtttt tagtatcgat tggtgtttct tttgctttta
    33301 cccggcgtgg ctggtaataa ttgataccta aaagatctac tgtattttct ttgataaccg
    33361 tcaaatcctc tgcatggatc acaggcatga gatcaagttc ttttactatc tcaatcaatt
    33421 cacttggaaa ctcgcctttt acagctggat caaggaatga acgattgaag aacgcatctg
    33481 ctatttgcgc tgctttaaca tcttcagggt ttgattcgtc tcttggatag cttggtgtca
    33541 gattcaagat gataccaata acaccacctt gctccatttc atggtaagat ttgatggcta
    33601 acgctgaagc caacgcttca tgatagccaa cttggatcgc ttttttcata tctgactctt
    33661 ctggataatg ccatcgatat aaataacctg cttcgactgg aacgattggt tcattatgag
    33721 taaaccattt ttttactttc gaaccaaaca attcaaaaca acttttggca tatttttcat
    33781 aggcttcaac cgtttctcgg ttcaaccatc cgcctttttc ttgcagatac atcggcatat
    33841 cgaaatgata cagattcaca aagggttcga taccgttttc aatcaactca tcaaaataac
    33901 gatgatagaa acgaacggct tcttcgttga ttgttccaga ttcatcaggt atcaaacggc
    33961 tccattggat agatgttcga aatgaattat gcccagtctg tttcatcaac tgtacatcct
    34021 cttgatactt cgtataaact tgcgaggttt tttcaggtcc tacttgatca aagaactttt
    34081 gcggttcttt tttgtaccaa taatcaaaaa tgttctctcc ttttccatcg cctgaaatcc
    34141 ttccttctgt ttgtgggcca cttgctgcag aaccccacca aaaatttgct ggaaattgat
    34201 attttttcat caaagcacct ctttttcttt tttagttcgt tttagttgat tccatgtatg
    34261 atattttcca tcacaatcac tttttcattt tcttcctctt cttttttgat tcgttttaac
    34321 agctgttctg ctgctcgttc cccgatttta tctgttggtg gcgtgatcgt gattttcgga
    34381 aggatactca gatcgttttt atctaaataa tcatagtgac tgaacgcaat atccttcccg
    34441 attctgacac cttcttgatt gaaacattcg atggcaccaa tcgacatatt gtaattgctg
    34501 acaaaaatag cagttgcccc tttttctaat aatgcttttg ccccgtaata gccgcttttt
    34561 ctagaatagt ctcctgtaaa gacctctaat tggatcgatg gatcattttg gaccgcttct
    34621 ttggctcctg ctaaacgttc tcttgctacg tagtctgttt gagtggcagc aataaatccg
    34681 atttttttat gtccttgttc accatgatga gcgatcactt ttgctacact ttctcgatca
    34741 ttcactaata ttgtatcaat acctgctggt ccttctggac aattcaatgc gataactgga
    34801 atactcattt ttgtgagcat ttcaaatcca gaccactctt cttcggataa gaagacaatc
    34861 agcccatcga tcacatgctt catcaggtat tcaatttttt gttcaatttt ttctggctga
    34921 tcggcaaacc cgcttaataa taagctatac ccattttctt cagctactct ttcgatccct
    34981 gagacaactt ccgacccaaa acggctggaa atcccgttta tcaaaagtcc aatagagaag
    35041 cttcgattat tcttcaaccc tttagcaatg atgttttctt tatagtccaa cgctgaaatc
    35101 gcttctgcta tttttttcat gttcttttcc ttcagttgct gaccattcaa gtaacgggaa
    35161 actgttccca ctgaaacacc agccatctta gccacatcac ggatcgatgc catacattct
    35221 cactcctgcc agttacattt gatttctctt tcttgttgtt cgattatgat gaccgcattc
    35281 tttgcatagt gttgcttgtc atcaatatcc atataactaa agtcttcagt agagcaactt
    35341 cctctcacta ttaattatac ttaaacaagt atttgaaccg gttcattttt aatatagcat
    35401 ttttttaaaa gattgcaaca cttttttaaa acgctttctg aaagtggcgg attttttgcc
    35461 tttttaaaat tttattctaa aatttactct atttgtgggt gttttgtgcg tttttctctt
    35521 ccatccgttt gaagttttac attccaatgg tataatttct ttatcctatc aaatggggga
    35581 agtattttga agaaattaat cgtactattt attttgtctg cgggactttt gacattgact
    35641 ggctgtgatt tgtttggcgg ctctgaccaa tcctcggacg aaacaaccga ttcttctgtc
    35701 ttaaaagcgg aaaaagcaga taccaccaac agtgtatata cgctaaaagt acgtaaagat
    35761 atggatttat ccgcgggaca gttgacattt acagataaaa aaatggaatg gacacgcacg
    35821 tatcagacac aaggaactga ttcttctgaa tcatctgaag aaaaaacagt gctgacagat
    35881 atcaagatca ctaccaaaga tgacgaatat attatttcag gaaaagaaaa taaaaaagaa
    35941 acaacagtca tatttacaaa aatcggtaat taccgtttga aggatgagga tggcaacatc
    36001 tacagtctat agtgccgtct gttggatctt cttttgtttc tccccttctt ttctgctata
    36061 gtaaagaagc aagatgattg taaaggagac gaaatggatg atcgatagtc ctgattattg
    36121 tacatgtaat aacaacaaaa atatccatac agaatttaat gaccaaggaa gcttgagcat
    36181 atgcgacaac tgtcacaaac tagtcaaaga ttcatttaaa acatttgaat cttatcctgt
    36241 tcaaaattat gtaaaaagta gaagaagata aacaaatcca agcaaaactc actatggctg
    36301 atttttatga acttcaaact cagcttcttc ctttgatcaa gcttcatcag taaaacaaac
    36361 agtggatcat ttcagtttac aaatcaaaga gtaaagattt atttatttca aatatttgaa
    36421 tataaaataa ccaaacgata gaaaggctta ttctacatca cgtagtaatt tttctttccc
    36481 gtcgtttggt tttttatttt tagcctaatt cttgaccttt tgcatgctgg ttatccgtaa
    36541 aatattttcc agcaggcttt ccttccaatt cagctaattt ttcttggatc tgacggatca
    36601 ccttttttgc atcttctgga ttttctacaa aatcgaattt atccccatcc acttgtattt
    36661 taggacatac atcaaaatct tcataccatt gattatagcg ggtgttcaac gtttcataat
    36721 aggtatataa cgtctcatcg ctttctagct gttcatagtc acgcccacgt tttttgatac
    36781 gttctagcat cgtgtccaaa gaaactttga tatggaccat caaatcaggc cgtttttttg
    36841 gagcaacatc atctaactcg tttagcatcg tatctaataa gctatcgtac tgctgtactt
    36901 cgatatccgt tacccgacct aaatcagcgt ttagatggaa caacaggcta tcttcataga
    36961 tggatcggtc tagcacattg tcatcgttca ctaaagcatt tttcattgct aaaaatcgct
    37021 tgttcaaaaa gaagatttgc aataaaaaag cgtactgctc aggatttgaa taaaagagcg
    37081 gcaatacttc gttatcttcg acattttcat aaaatggttt agaatcaatc tcttttgcaa
    37141 gcatatccgt caaactgctc ttacctgcac caatcgttcc tgaaatcaaa atcactgaca
    37201 tacaaccctt cctcttctat aagtactcgc ctacgtaagt ttcaacaggc aatgtttcaa
    37261 aatcatattc acgtacagct tggtctgatt ttaaatacgt atggagactt tgtgtaatca
    37321 tcaagttcgt aggtaccgtc tcttcttgta caatgatcat cggttttttc aacatagttg
    37381 ccacacctaa ttcgtaagcc gttcctggat cgattgtttg atgctcaaag tcaaggatag
    37441 caacaacaaa ttctgcattt gtcagttctt ccatatcttt ttcgtatact tcctgcgccc
    37501 atccagcgct gaatagttca taattgcttt cttggtgatg acgtggagag tagaaacttg
    37561 tgactgtttt gttttcctcc aacgcttttt cgatacgact tactcgatcg atttgctcct
    37621 ctgaaaaaaa tggtcctgct aaatatattt gactcattat tttgcctcct tcaataataa
    37681 ctgattttta atagatactg ctcgtaattc attgaccagt tccaatggat tgttggaaaa
    37741 gattacttga tccaaccatt ttttatccat aaaatcctct tttgcaaaag tttctaataa
    37801 ttgttgcaag ccgctgtaat aaccgccaat attcaatatt ccgattggct tgtcgggcgt
    37861 cagaccgatt gctatccaag aaagaagttg tgagaattcc tccaatgtac ctagacctcc
    37921 agggaaaacg aggacagcat ctcccttttc aaataataat acctttcttt catcgatcgt
    37981 ttctgtagga ataaatgtag taacatcttt tccaggcaat tcagtttcaa aaagcccatt
    38041 cggatagata cctgtgactt ttccgttatt ttccagtact gttttactaa tcactcccat
    38101 aatcccggag tatgaaccac cgtaaaccag ttcgatttct tcttgtgcca tgtattttcc
    38161 taacgtttgc gcaataaatt tgtaactttc tttattacca aaacgggaac cgcaaaaaac
    38221 agtaagtttc ataaaaaaac actccctaac tcaaaagtct atcttcattc tacacgtgtt
    38281 tttttaaaat aaaagactag attttgtagt tttttagtgg tatataggat agacgccact
    38341 atatatagta tttagaaaaa aatcattttt cagccatttt atagataaaa caaaaataaa
    38401 taacgtgttt tcatcgatat gaaatgactt acttatagaa gatactggga tttcgtccta
    38461 tatgccaata acgtgtatta ttttaaacaa caaatacttg tttcttttat taacgaaaaa
    38521 atcattttgt tctcgtgtta tgtttcactg ataaatgatg tttctctaaa taaaaaagcc
    38581 gacgaactaa acgatcaaaa tttgatcatt tttgttcatc agcttctcct aaaaatcttt
    38641 ttgttaacga taaatagctg gatttgtaaa aatatcactt gaatcgtcac tgctggaaga
    38701 aaattcagtg ataaccgcac ctgttgcgcc tgcctggaac tagtgtttga tattgggctc
    38761 gaacgtgtac tgatctctag catgaagaat aatttctttt tttactgtat aataactttc
    38821 tttccttttt ggaatagtac aggaaagttt ttttgtttcc tttccttcga cataaggtat
    38881 accagaccct ttcgacaacg aaaggttctc atttttcctt catcgtcatt tcaaggaggg
    38941 tgccgatgct ctggacagtt tcgatcgctc ctaaaccaaa atctgtatat tcgattcttt
    39001 ctaactctga ttctttcata ccattcctgt ctcaaggtac atctattgta cttttttctt
    39061 gtaatccatt ctgcctctcc taactaattg tcaaatcatc ccaagatatg tcttcttcct
    39121 ctttttcttc ttggatgatt tctgcattcg gatctactgg ttttttcaat aataaaatgg
    39181 ttattcctgt gacaaaagaa ccagctaaca atgcaccaat gaatgcccat ggtctggtca
    39241 tcgtagcgac tgcaaacatt cccgatgccg gaatacggct atctgcgccc caaaacatgc
    39301 ttaatccgcc acctactgtc ccaccgactc ccgttgctat gacattgcgt gccaagtcgt
    39361 tcaatacgat agggaaacat ccctctgtga tctgacagat ccccatagga aaagcggtct
    39421 tcaatgtatc gatttcttga cgattgagta tatttttacg aatgattttc cctaagaaat
    39481 aagcgatcgt caagccgaat ggggttacca tcgaagctaa aatcaataca gtgattggtt
    39541 catagattcc ttctgaaaat cgtgccacac ccccttatcc agaataaatg gtaatccaga
    39601 aacagccgtg tatctgcggt aataagccta accaaacgaa aaatatgaga agctattttt
    39661 gtttggctgt tcttattact tgatgcaggg cagctttttc acaacctctt tttttattta
    39721 tgtttcataa tcaatatggt aaggatgcag ctccgtatgg tagaccgcct ccgaatattc
    39781 aatcatttcc ccaatttgcg tagacgattt ccgtatccgt ttgatgccga tctgtgcgtc
    39841 tgttttcaga attccttgtt cttcttctgt taaaaatact gccgagaaat catcagaaaa
    39901 tgagtcaata gtatatcctt ttttcgtcag caaacgatac aaagattctt tgccaaattc
    39961 ttctctgctg aattgcatca tttcttgtgg aagataatga acaaaataaa tataaggctg
    40021 attgttcaaa taatataagc gcttaaaaca aacagcttgc tcaccgaaaa aggaaaacaa
    40081 aggatcttcc ttctttatat ggaccaattg caaatccagc gtttctttcc ggacttcata
    40141 tcgggagttc tccaagattt gcgtaaagct tgccgctttg gataatcgat tgtaaggacg
    40201 attgcttaga accgtcgtcc cacgaccact tttctttgcg acatattcat ctgatgccag
    40261 cagttcgatt gcctttcgga cagtgatttt gctgacttga aacagttctt ctaattcact
    40321 ttctgtcggc aacatcgaac cgactggata ttttcctgag aaaatatctt ctttaatttt
    40381 atctgcgact tccaagtata gtacacgtgt ccttttcata acgtcaccct ttgctttact
    40441 attactaaaa gaatagcaca taaaaaataa aaaaagaaca caaatcacat gtgagaagtg
    40501 tgttcttttt cattcagctg ctttaattac aagtttacgc catttgaggc aattacttct
    40561 ttataccaat caaaagattt tttctttgat cgattcagtg tcccatttcc ttcatcgtct
    40621 agatccacat agataaagcc gtaacgttta ctcatttctc ctgttgatgc actgacaaga
    40681 tcgatacatc cccaaggcgt atagcccatc aaatctacac cgtctgctac tgcattagcc
    40741 atcgcttcga tatgacgacg taaataatcg atccgataat catcaataat cgtgccatcc
    40801 tcttccatat gatcaatggc tccaagaccg ttttcaacaa tgaataatgg tttctggtaa
    40861 cgattgtata attcattcaa tgcgattctc agcccttctg gatcgatctg ccagccccat
    40921 tcacttgcct caagaaacgg attttttact cctcccagca agttcccttc tgaagttgct
    40981 gcttgtggat cagtctcatt gattgccgta gacatataat aactaaatcc gatgtagtcc
    41041 actggatatt cttttagcaa ctggaagtct ttttccgttt gctctaattt ttccggatcg
    41101 actccatatt ttttatgggt acgcgctgtg tatgcagggt attctccacg tacttggaca
    41161 tccgtgcaga aaaaattgaa ttcttggttt tggatcattg ttgctgcctg attgactgga
    41221 ttggcatcga tgctgtaagt tgttgcatag ataatcatgc agccgatttg gatatccgaa
    41281 cgcaattcgt ggccgatttt tacggctttg ctacttgcaa cgaattgatt atgccatgct
    41341 tggaaaatat tttggtactc tcctgctcca ttgcttttta ctaatccttg acttaaagct
    41401 ggaaagtgaa aggccgaatt gatttcattg aaagtcatcc aatatttgac tttactgctg
    41461 tatctctcta atactgtctg agcaaatcgt tcatagaagt cgatcagttt tcgatttttc
    41521 cagccaccgt attcttttgc caaatgaaga ggcatttcgt aatgagaaat cgtgatcaca
    41581 ggttcaatat catattttaa gcactcatca atcagctgat cataaaattc taatcccgct
    41641 tcgttaggag taccttcgtc tccatttgga aaaattcgtg tccaggcaat cgaaaaacga
    41701 taacatttga atcccatttc cgcaaacaag gcaagatctt ctttgaaacg atcgtaatga
    41761 tcgattcctc gatgattcgg gtaaatatat ttttcttgat caatcgtcca gtcaaattct
    41821 tcactgccga taatcgcaaa gcgctgctta cctccaggca tagcatctgc aacggatagt
    41881 ccttttccgt ctcgatcata agccccttct aattgatttg gcagcagttg ctccacccca
    41941 taaaaatttt tctgaaaaac gtgattgcat cctgatttcc tccttatttt acaattgcca
    42001 gaacatcttc gtttgcaata atctcttttt ggtctaattc aagtatatcg gcaaatgcat
    42061 cagtattcgt aatgacaact ggagtgatta cgctatagcc agctgctcta attgcttcga
    42121 tgtcaaaacg aatcaataaa tctcctgctt tgacgagatc cccttgtttg actaagatct
    42181 caaagccatt cccatttaat tctactgtat ccatcccgat atgaatcaaa atctccgctc
    42241 cgtcagtcgt tgtgatccct acagcatgtc cagttggaaa aatagtcgtg atctctccgt
    42301 ctgcaggtgc aactagttct cctacttctg gatcaatagc gatacctttt cccatcacgc
    42361 cagaagaaaa aacttggtct ggtacttcac tcaatggcag tactttaccc gttaaaggac
    42421 tttttaaggt atgccgtgct gaagtaatcg gttttcctgc attcgcatgt ttgttttcta
    42481 attgattgtc ttgtgtttgt tcgtcaaatc ctaaaatcaa tgtacctacg aatgcaagaa
    42541 caaaagcaat tcctgtagct attactgcta ctgtcacatt tgactctaca ccatccaccg
    42601 tattaatgaa agtcggaatc gtcaataagc tgaccaaact gctggagaaa gcttttactc
    42661 cggaaaatcc gataatagct ccaccaatac ctccagaaat acaagcggca atgaatggtt
    42721 tcttcaaagg taatgtcaca ccatacacag ttggttcagt gattccaaat aacgaagtca
    42781 ctgtagaaga aatccctaat gctcgaagtt tcgtatcttt tgttcgcaat gctacagcta
    42841 aagcagcccc accttgtgcc aagatggctg gaagaagcat tggcatcaat acatcaaacc
    42901 catattgttc gatattcaaa aacatgatcg gtacgaatcc ccaatgcatg ccgaacatca
    42961 cgaatacttg ccataaactt cccatgatta gacctgctac gattggacta aatccataaa
    43021 tcgagttgaa gagaccgcct aaagcattac cggcaaccgt cccgattggt cctaaagcaa
    43081 gaaatgttaa tggaaccatc actactaaaa cgacaagcgg aactaaaatc atttgcagaa
    43141 attgcggaat cacttttttt acgaatggct ctaatttact ttgaacccat acggctagaa
    43201 tgattggtat gacagaagaa gtataaccag atggtgaaag aatcactggg atcccaaaga
    43261 aactgatagc ttctcccgca ccagcaactg ctgtgatagc tggatacaca agtgccatag
    43321 caattgctac tgctaaaaac tggttggctt tgaacttttt agctgctgta aaagcaagca
    43381 tgacaggtaa gaaggtaaag acaccatctg ctgcagcaaa caatatttga taagtaccgc
    43441 tatcagctgt cagccagccc atcaccgttg ccaatgataa gaatcctttc aataccccgg
    43501 cggctgccat cgcccctaga aaaggtgtaa atatactaga gataatatcg atcagacgat
    43561 tcaaaatatt tttggactct tcttttccat cctccgaaac tgcaagacct tcctcatcca
    43621 cgatcgattg ataaacatct gctacattac tgccaatcac tacttgatac tgaccgccac
    43681 tttgtacgac ttggatcacg tccgggtctt cttttaactt ttgcgtatct gctaaagatt
    43741 catcttttaa tctgaatcgc aaccgcgtag cacaatgtac taaacttttg acattctttt
    43801 gtcctcctac cgcttcccat acacgacgac caactgcttg attatccatt gttttttcct
    43861 tctttctttt ttatttaaaa aataaaaacc cagattgaaa aaaacagtca caaacattcg
    43921 tgaacctatt ttttcaatcc aggttttgcc tgccatacag taacaatcct tatgattgga
    43981 tgcttttttt ctcacttact actctttgaa tatgaatcgt taaataaatc tgctctgcga
    44041 tccccatttc ctgtttttgc gtttgtcgaa gatactcatt gatccgttcg acacaatgaa
    44101 acgcttttgg atactttcct tgaactaatg cgtagagaaa ttcatcttgc tcgtcagatc
    44161 cttccccttt gagataacgc tgggcaaaaa actgcagatg agtaatgata cgctgataat
    44221 tcaaagattc ttcatcgaac acgatgccga agaatttact gatgatactc agtatatctc
    44281 ttaccattgt ggtagcggtc atcgtcactt gcatgtcttc gtttccttgt tggctattca
    44341 caaaatgaaa ggcaataaaa ccggcttcat cctcagaaag ttccacaccc gtcttttgtt
    44401 tcacgaacaa aagagcctct ttcgctgctt gatattcttt cggataaaat ttttttgttt
    44461 caaataataa tggattaggc agttggatat tttcttttgc tcgtgtgatc gcataatgaa
    44521 gatgatcggt caatgtcaga taaatattag gcgaaaattc aacttttagt ttatcttcag
    44581 ccagttcgac tacttgtttg acccattcga tctcctctgc cggaatatca gccatcagct
    44641 gctggaattg gtttttagca aaagaatctc ttaaaacaaa ttctttttgg accgcttctg
    44701 gcgaaatctc gtccccaatc tttttttgaa aagctagtcc ttttcccata caaacgactt
    44761 cttccccgaa tccatttttc gaaatgacga cattgttatt caaaatttta tcaattttca
    44821 ttttgcttcg ccctctcaag aaaaataaaa aaacctagaa ttcgcctact gtttcttagc
    44881 tgaattctgg ttttgcctgc cgtacagtaa caatccattt cttattcaaa atcatcatac
    44941 catcgaattt ttatttatgc aagccttttc atataaaaaa taaaaaaact ggaacacaag
    45001 tctatctaga aaacaaccaa atcagagatg cacctctttg cgtttctctt tttttcagca
    45061 aattggtttt tccttatcga ttcatatcct ttggctgttt ttaatctgta acgacttatg
    45121 tcccagtatc ctaaaatgtt tttgtctcta gattgaaagc agatctcata tcatctgtcg
    45181 catcttttat cgatgtaaca ctttccagtt ctccttgtac aactagtcgc gtgacggcgt
    45241 ccatgtcgcc acaagtaatt agtgtgacaa tttcttttcc ctcgacttca ttcagtaatt
    45301 gggtatctgt aggagcaact ttttctttaa aaaatgtttt gtatgtatac acattttcta
    45361 aatctgtcaa atagatcttg gcacccatct ctaaattttc tagcggtgta aacagcaatt
    45421 cagggttcgt tgcacgatgg cttgctaagg cataatttcc ttttcccatt tcttgatctg
    45481 gagataatgt tcctgctccg taaagtagtg acgtattatc caagcctttg aaaaccggta
    45541 aattgattga aactgaaggg actgcgatac tagcaatcac tggtagatct gttcctctca
    45601 tttgcgacct catgacccct tctgtcgtca ttggttctac cgcatcaaag tcaaaactga
    45661 catctttatc gttattcttt ttcagatctt cttttgttac attcgctata gcatatttat
    45721 ctcctgtctc tctgacaaaa taatccttga tctgttcatt gaaaatcaaa gctagtccta
    45781 ctaataacaa gaggaacaag aggatattga ctagccatct ttttgctttt cctttttttt
    45841 tcattttctc tccctccaga ctcattttac tgtttttctt ttgctctttc caacagccga
    45901 atgacacatt tctacaactc tgtgtatttt tttcgtacac ctgcgctttt ttctacagga
    45961 tatttcaaat tatttttgat caatttttct tcaatgatct catctagatc caggtgaagg
    46021 ttgtctgcca tcatgtatga atagatcaat acatctgcca gttcttcttt gattcgttct
    46081 aattgtgttt cttttacttc ttccggcgtt ttccattgaa aaagttctaa caattcagat
    46141 gcttccaaag aaatagagat agccaaatct ttttcgttat gaaattgtcg ccaattacgt
    46201 tcatctctaa actggttgac tttttccata ctattcattt gcttgccttc ttccaatcac
    46261 ttcttacttg attaatcata ccattttttt acataaaaaa tagatgatgc ttttaaaaaa
    46321 actcctgttt tcttttattt aaaataaatg tcatagaaaa tcaataattt aggtagccac
    46381 catgtgtatg cagtagcgtg tcttcttccc ttgaggcttt ttgtttttcg atacgttctt
    46441 cttcccaaac agacaacgct actagaatct ctcttaatga ttttccttct tcggtgagcg
    46501 agtactctac tttaggcggg acttggttat aggcttttcg atggatactt tcatcttgtt
    46561 ctaactctct taactgctgg gtcagcattt tttttgtgat cgtagggatt tgcttgcttc
    46621 tccacgccta aaataagcga tattgctctt ccctttatct ggtgtaagca aagcagttag
    46681 cattttgacg atcgtgggtt ccgcttttca cttcttatct acttagtatc atgaagtata
    46741 ctttcgtact aatactaagt agcggaagtg tcaactactt tgtttaacta catagcaaaa
    46801 gaaacagaag ggtgacaacg gtcatcacgc ttctgtttct tttgaatatg ctgtgttgtt
    46861 gtctctatct ccgtgtagac tttcctcaaa cttatccttt gacagtaagt cagtaggctg
    46921 atttgctctt tctagatgcc agatacattt ctacaaaaaa atagtgattc tgttttgaaa
    46981 attcgatttt tagttctgca ttttcgtaaa catccaacat ttttttcgca ctgtatagac
    47041 ctaacccgcc atttttttta gaggtttttc ctcttttgac catctccgat agcgtcaaat
    47101 tcgttggatt tgccttgttc gctatactaa aacgtgatcc ctctttttct tccagcagat
    47161 tgatcgtaat cggactctga tcggctacac gatgatcgaa tgcgttagaa ataaaaatag
    47221 ataaacagcg aataaaaaca accagatcaa tcgggattgt tttcacttcg acaggaataa
    47281 ctacttggat cgggattttc tccgattctg ctttttctag aaattttaaa aagacacccc
    47341 tgacagctgg ggatacaata tttgttagct gttgtatttg cggtttttca aaaatagctg
    47401 cagaatgatc ttctaatatt tctaaaaatt ccaacgtttc ttttttattt tcctgaagag
    47461 taatttttaa tgaaaacagt agacttttat aatcatgttt gaattcctct agatgatggt
    47521 aatattcttc atttatttga tacagttgaa gcgattcctg atgttgtctt tcttttactt
    47581 ggaaactgat caacataatg gaaaaaaaca aagaaaaaaa actgatgact atcgacaaaa
    47641 gagatagaag cagataatta gatgagcgga agtgaacact tcccaatacc aagtaaatca
    47701 cgccccagct taataaaata caaatacctc caaaatagtg ttcagttgga taagtaaaaa
    47761 gcaagttgag aaatctcgtc tgtttgtcta tactttttac taatagatag aaaataaatg
    47821 cgataagtag tgcaaaaaaa cttgctgttc caagatgtct ctttcctaaa taatccatca
    47881 gatcaaaaac cagaaaaaag ctgagtccag tgatagcaaa aaaaagatta ctgatcaaac
    47941 agctgataaa caggtttttc gtagtaaaat aaaaaagaaa catcaataat aagccaacta
    48001 ggtaaggaaa gctcacttta gggataggaa gtaaaagtgt caccaccatc aaagctatac
    48061 aaccaaagct gttttttact agcagttgtt tcgaaaagaa taaatagata cctaaatcta
    48121 aaatcaaagt aaaggtcaaa taaagtgtca ctgttgcgac catcttattc ctccattgtt
    48181 ttgtttcaaa atgtacacat atttttttaa taggagcctg catatgaaaa agtattttat
    48241 catagaagat aaccagtttc acagcgaatg gatcatcaga gaattggtca atcatcatat
    48301 cgagaatatc gtgcagctca cttctatctc tcaaatcaat cagttgatcc agttgggcac
    48361 tattgataat cacgatgttt tctttatcga tatcgacttg aagcaatcac tcacaggtat
    48421 tgatgtcgcc gaacaaatac gtaaaaaaaa tgcacatagt aatattgttt ttttcaccgc
    48481 ttatacgaat tttgcagtag agatcatcaa tcggaaaatc cttccgattg gatatatcag
    48541 aaaagacagg aatttatctg aacaaattcg tcaagtgcta gaacagatca attggaaaga
    48601 acagcagatc gtaaatgtcg ataaaatctt tattgatcaa aaaggtggaa gtctcgtcct
    48661 gtatcctcgg gaaattttat atattgaaag tatcaaaggt ttgaaaaacc aagttatggt
    48721 caaaacaact acagaagata aattagtgaa tacttcttta aaacaattga agacagagtt
    48781 agaaaagtat gcttactttt tacttttaaa atcttactgt atcaacttat cgcaattgca
    48841 gaaaattgat tctgctcatt atgtgttaga atttttcaac ggtgactcct tactcgttgg
    48901 tcgaaagatc tttgaaaaga caaaggaacg tttccatgac ttccaaaaaa ctgcttcgag
    48961 ttgatgaata agagctgaga cgtaagcata gcagatttca gggagtgggc cttgaatcgg
    49021 ctccactccc tgaaatcgtt gatatgattt tacgcttcct cttctgtagg agcatctgtt
    49081 aacgaccatg tgaatgttga aacgtagttg tctacagcat aattggatac aggaacaaat
    49141 aattccactg attgtctccc ttgttccaca ttctctccta atgaataaat ccatgtgcca
    49201 atcccttcat ttgctcctgc agttactaga tctactgcct gatctgctgg gatttcgatt
    49261 ccttcagcgc cagttccaga aacggtagat ggcattccac tgctattagt tgcgctattt
    49321 cgaatcacgg cgtttttgaa tttcaccttt gaaccagtta gtttattcgt atcagatgtt
    49381 gtaccaataa attctgttcg tttgactgat aatttccaac cggcataatt tccgctttta
    49441 tccgttactt gtacgaaatt ctgtacttgt gtttgtgctc cagtactgtc cgttccgctt
    49501 tgtaatgcag catatactgt ttttgctgta cctaacttga ttgttccaaa atcaagatct
    49561 ggagcttggt caatcgtcag taatccggat gttccctgat ttggttcgtc aggtgtaaaa
    49621 ggagtatcag gatcatttgg atcaacagga ggaactggaa caacgtcttc aggatttgca
    49681 gcatctggtt tggtcacaga aatcgttcct tctgtagtgg ctgagctctt attctcagca
    49741 aaaacacttt gtgtacataa taatagactt ccagctagaa cggtacttaa tgcaattgtt
    49801 tttttcattt ctttttcctt ctttctttta aaataaataa tccagcaaat aagccaataa
    49861 gaatgactcc aacaagtatc agccatcgcc agtcaaatgt ttctggctga ttttttaaat
    49921 caatatcctt ttcattcaat tggtccgctg catcatcagt tatttcaaaa tcggtatgga
    49981 gattccagcg tttttctttc caagcaccct tcacaaccgc ttgataatgt cccggagcta
    50041 atttttttcc ttctaatggg acaagtaaat caaaatttgt attgggtgct acatctattt
    50101 tttctttttt tattgaaagg taaggctctt ttgtttcttt tttgtagatt tcaaccgatg
    50161 tagtcaagcc gaacatataa gaagcatttg gattttgaag attcgcctta atcacatttc
    50221 ttccatcgct ttgatccgca taagcttttt ttaatttcaa atctggggaa gactcttttt
    50281 cgctatttca aatcaagatt gctttcactt gagaaaactg attgacgaca ccaactgtac
    50341 ttttgttaga aacgttctct tttttttctt gaaacgtgat ccctcctgcg accacaccag
    50401 ccagctcttt ttctggcgct ttcagtgaaa gtgtctcagt gaccgcactt tgaggaggaa
    50461 cagttatttc tcccactgtt tttgctaagt ttttcatatc ataacttaat gtttcatcaa
    50521 atttactttt tgattctcca tattcgatca caccgtttga attcgtcgta gcggagtgga
    50581 tcaatgtctc aactgttact gatttatctg ttcggttaaa gaccgtgtag gggatttcct
    50641 gtgttttccc aggctccatc cttacattta tatatgtttt gttcttatca atctgattag
    50701 ccggtaattc aaaatctacc gcaaaattga attcggaggc atggacaggt gtctgccaaa
    50761 ggatgaatcc aaggagaagg atcataagta aagagcagtt gtttcgatga cttttattcc
    50821 atattcgatt catcaatcat cctccttgat ctttattctg tcggcgcatc tgctaatgtc
    50881 catgttatct ctgctgagta attaccagct atctgatttt ctttggttaa gacaagttta
    50941 aacgcccgac cgttatttaa atattctgaa taatctgctt gcacattcgt tgaattttgt
    51001 cctctgacaa tttccagttc ttcttcattc actaattgat actcctctcc tttcttgaaa
    51061 tataggacat cctgcataga aaatcctgca tcatttgtga aaggctgaga taattttatc
    51121 gttaagatcc acggattttt cgtttctcgc tgatcataaa tccataattt ttcctgtata
    51181 tccatttggc caaatgcttc ttgtgtcaaa ccagttgctt tgatctgacc aaaatcgagt
    51241 ttggaagggg caagaaatga gagaccacct tctactacga tcttcgtagc aggtgcaaaa
    51301 ggcggggcat catgataaac aactggcgta tcacttggat tgtgattccc aatttcatcg
    51361 ttggtacttg gcttgttctg cacaccctta gcagtagctt ctcctgcttg gtcgtttaat
    51421 acaatttcta atacatctcc tgcaaaaagt tgattttctg ggatagaaag aacaaattcc
    51481 cctgtatcat caatagttgt ttctgacaaa acagcttgat tttttttcaa tgaaaccaag
    51541 gctcctgctt cacctgtacc tgtgatcgtg cgagaagctg cattgatcaa tgtggtactt
    51601 aaattagcag gttcaggagg aatcacatcg actgtcgttt tggcagagtc cagttctgtc
    51661 acttcttggc cacctcttac ttttttagca gcaacaactt gcacttgatc ctccgttttt
    51721 aatctctcat cattgggtac ttgaacttcg aacaagccgc cttgttcgcc ttctccccat
    51781 gggttatatc ctgccccttc attttcatct gtttgtcctt gcgtgagagc agtaatttct
    51841 acagatgatt tcgtcgtatc tgcgtaagta atacgtaaag tcacttctac ttcgcctgtc
    51901 caagcatctc ttggctttgc ctcgacacct tcgggaacaa cgacatgccc atagattttt
    51961 ttatctgcat tcgttggcac tcttaaggat tcaatcatcg ggagctgatt attggcactg
    52021 attcgattat aatttttcat tccggcacct gtctgagcaa attcatttgt taaacctgca
    52081 ctagggcttg atacgaaatt gcctaagtcg gtcccattga tcgataagtc tgtcatatca
    52141 gaaaaataat ccgggtcttt ttcaaagtta tcgaatgttg cgcctgtttt tcgccataaa
    52201 gcgatttctg aatgggcgat ctttaaagaa gaatctccat ttccttggaa tacccagccg
    52261 cctatgccat tggtctcgct tccaatccgt ttgttttgga aatcaaaata ggtaggagat
    52321 tctaagtcca cattcaacac atccccactg atgatgccgg tcttatcttc ccctgttttc
    52381 ccgaccatga ctaaataagt tttttcacct gccgtaaaat ttaaatcaat atctccagtt
    52441 gtatctacag ttgctccgga atctgcgcgt atatcgatat ttgatttttc tccagtgact
    52501 gtaaaatcag ctgaacctgg actttctaga aaaccagaag cgtcataaaa ttggatcgct
    52561 tgatttcctt gattgagtct gttcacgtaa gatttgccat cacctgtatt tcttaccctc
    52621 aatgagcctc cattggttac tgtcagcttt tgcgtaccat tctcaaaacg aactgcgctg
    52681 gaacgcccac tctgcttatc gatgtcgata actgatccgg ctccttctac gatcaaagaa
    52741 aggccttttc ctacaaaacg catcccattg ttcccgggtt gatcgtcatc tccagccatt
    52801 ttgaccgaga tttgtccacc acttgataca tggatttgac tagaatctcc ttgcaagcga
    52861 aatccgcccg tagttgctgt attgatggtg attttcccac cattcgatac agtgactttc
    52921 gatcctgaac ctctcatttg gatgattccg cgattgttgt cattggtaat attgatcttt
    52981 gtatttaccg aaaattcgct tcccgaacca ctgacttcaa acatgacatt accaggtagc
    53041 gtatcgactg cctctccttc atcgctaacg ccatctgccc gatcataatc aagagagacc
    53101 tttgaaccgc ttaagatatg gattcctggc gctgtttttc cactaccagt ctcatctcct
    53161 gaatctagaa aaccgtagaa tttccctaga tacggtccat aaagttgact accattgatc
    53221 acattcaatt gatagcttct acaaaaatag gctttacccg gagcggcatt cagtccttcc
    53281 ttcactggtt ggacatccaa cacaccggaa acaccatcta atgtgacagt tgcacgagga
    53341 gcgtaaatca gtcctaattt ggacccatca tacaaatcaa gtgtcccggt aaattttatt
    53401 tctcccgtaa aatgatatct gcttccccaa aaatcggtca tataagcaat gataatggca
    53461 tttccatcga tatttggtgt caatccacct ggatgtgcag ctgttccttt aaaattctgg
    53521 aactcgatcg actgaggtat ctcattgata taaatatggt gtgtgccgaa attgtgagcg
    53581 aatccaccaa aatcaataac gatatctcgt ttgatctcta attccctgtc taaagtcaaa
    53641 ggctgaatca gtcggatctt agttactgat tcgtctgcga gaagctgcgc cagttcttct
    53701 gttgtggcag cttcgctgta cccatccgct gaaacagctc ttgccagtgc cgcttcactg
    53761 ctatcagacg tttgttcagc tacttctgag gtctcacttg tcttattttc ttcttgcctt
    53821 ttattagtag aagcctcact ttgagattct gtagtttcag aagtaatcga ttcttctgaa
    53881 aaagtagtct tgtcaaaact ttgattctct gattgcgttg attcgtttgt tttctctaca
    53941 tcggcaaaaa cggatgtcac catcaagaga gggaagacaa acaaaatcga cgcaatcagc
    54001 ttttttacac attgattcat tctccaccat cctctctgac gttttgacaa ttttatttta
    54061 tcgttttaat ctatttccaa ttgtactttc atatttaaaa tatgtatttt tatggaaaaa
    54121 tttttctatt aggtgtaaat ttcttttttg tacaaaaaaa aggctgtgac aaaagtcttg
    54181 tcacagtccc ttatcccgaa caagcggtgt tctcccgcca actcctcggt cacaacctcc
    54241 attttaacaa taaacctgta aatgtgaggg caatactttg attgtcacag gtaatttatc
    54301 cccttcatct ccatcaacat tcgtcgtcaa ttcttcctca tcagctaatg acacaactaa
    54361 ttgctcacaa gtaaggtagg ctagattttt cgtagattca tctactcctt ttaataggtc
    54421 tggcaatgtt ttaagcgtat cgatcaaaga agaatctttt aaataaacca aatggagctt
    54481 accatcatcc acttttgcat ttggaagtaa tgtttcaaaa ccgccgactg agttagttag
    54541 tcctatcagc aaggtactgc tctcaatttc ttccttcttt ccatcaactt ctacgtgaaa
    54601 cgagtaggat tgtgtggaag ctaactgttt gattcctgac atgaaataag ctagttttcc
    54661 aaatttcgtc ttcttctccg catctacatc attgattgat tcagggatag agccaattgc
    54721 gacgacattc ataaaatagt cgtcatttat ttttccaata tccaatggct tgactgattc
    54781 aatcgaaaaa tgattgatcg cttcctcagg ttctagcggt atccctaatg ctcgagccaa
    54841 gtcattcacc gtacccaatg ggaaaaatcc gaaatttggc cgatgttctt gttcagcgat
    54901 tccactgatt ccttcgttga ctgttccatc tccgcccatg acaaacacac tatgataccc
    54961 ttccgtagcc gcttcgcgag taaagttttt tgcatcccct gctttttttg tatgcaagac
    55021 gaccacttca tcaaacaccg attccaattt tgctatcgct aattgctcaa attcctttgc
    55081 ttgttcaccg cctgaactgg ggtttaccac taataaaact tttttcatca ctttctttcc
    55141 cttctgattg atttctttaa tataacgata gcataaagaa aaaaattagg ctgtgatgaa
    55201 gtactgttca tgaaacattc atttattttt tgacaagata aaacatccat actatgaaac
    55261 tgaggaaaat taaaacaatg atataggaaa taaaagaaaa tgataggcat tgtataccta
    55321 ataaagtaaa aatcttaggt ggcagataaa aacacaaaaa tagcaagaga atgtaatagc
    55381 gtttcttcca ctttttgtta tcagacctta tagcgatcag aggaatacca aatgcataac
    55441 tgaaaaactc ttgatgtact gttaatatgt cacggccttg aataataaat gcgacaatcc
    55501 ccgctataat tagtgtgcct aacaccaaaa cggaaaattg atgaatatat tttatataca
    55561 aatgatccat tgt
//
LOCUS       DACDTO010000010        53851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDTO010000010 DACDTO010000000
VERSION     DACDTO010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 53851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.010"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            11..733
                     /locus_tag="IWK70_000635"
     CDS             11..733
                     /locus_tag="IWK70_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9613205.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            791..2830
                     /locus_tag="IWK70_000636"
     CDS             791..2830
                     /locus_tag="IWK70_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9613206.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2939..4090)
                     /locus_tag="IWK70_000637"
     CDS             complement(2939..4090)
                     /locus_tag="IWK70_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9613207.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4244..4774)
                     /locus_tag="IWK70_000638"
     CDS             complement(4244..4774)
                     /locus_tag="IWK70_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613208.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRLRKMTIR"
     gene            complement(4873..5223)
                     /locus_tag="IWK70_000639"
     CDS             complement(4873..5223)
                     /locus_tag="IWK70_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9613209.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWK70_000640"
     CDS             complement(5380..6909)
                     /gene="ahpF"
                     /locus_tag="IWK70_000640"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9613210.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWK70_000641"
     CDS             complement(6919..7482)
                     /gene="ahpC"
                     /locus_tag="IWK70_000641"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9613211.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7626..8288)
                     /locus_tag="IWK70_000642"
     CDS             complement(7626..8288)
                     /locus_tag="IWK70_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9613212.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8472..10118
                     /locus_tag="IWK70_000643"
     CDS             8472..10118
                     /locus_tag="IWK70_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9613213.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10109..10237
                     /locus_tag="IWK70_000644"
     CDS             10109..10237
                     /locus_tag="IWK70_000644"
                     /inference="COORDINATES: protein motif:HMM:NF015727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613214.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDHRKKRLP"
     gene            10204..11268
                     /gene="uxuA"
                     /locus_tag="IWK70_000645"
     CDS             10204..11268
                     /gene="uxuA"
                     /locus_tag="IWK70_000645"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9613215.1"
                     /translation="MDGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPRERIKEL
                     KEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVICYNFMP
                     VFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEPERLAHI
                     KSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLPRIVKNR
                     EDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRNIKFLAN
                     RDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGLYDRALG
                     AVYLNGLWEAIEKGNKSKDN"
     gene            complement(11376..12929)
                     /locus_tag="IWK70_000646"
     CDS             complement(11376..12929)
                     /locus_tag="IWK70_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9613216.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            13077..14365
                     /locus_tag="IWK70_000647"
                     /pseudo
     CDS             13077..14365
                     /locus_tag="IWK70_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14491..15570)
                     /locus_tag="IWK70_000648"
     CDS             complement(14491..15570)
                     /locus_tag="IWK70_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9613217.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15673..16113)
                     /locus_tag="IWK70_000649"
     CDS             complement(15673..16113)
                     /locus_tag="IWK70_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9613218.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16170..16580)
                     /locus_tag="IWK70_000650"
     CDS             complement(16170..16580)
                     /locus_tag="IWK70_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9613219.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16760..17194)
                     /locus_tag="IWK70_000651"
     CDS             complement(16760..17194)
                     /locus_tag="IWK70_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9613220.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17294..18142)
                     /locus_tag="IWK70_000652"
     CDS             complement(17294..18142)
                     /locus_tag="IWK70_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9613221.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18531..20297
                     /locus_tag="IWK70_000653"
     CDS             18531..20297
                     /locus_tag="IWK70_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9613222.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20399..21658
                     /locus_tag="IWK70_000654"
     CDS             20399..21658
                     /locus_tag="IWK70_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9613223.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21805..23106
                     /locus_tag="IWK70_000655"
     CDS             21805..23106
                     /locus_tag="IWK70_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9613224.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23109..23960
                     /locus_tag="IWK70_000656"
     CDS             23109..23960
                     /locus_tag="IWK70_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9613225.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23973..24788
                     /locus_tag="IWK70_000657"
     CDS             23973..24788
                     /locus_tag="IWK70_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9613226.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24939..26105
                     /locus_tag="IWK70_000658"
     CDS             24939..26105
                     /locus_tag="IWK70_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9613227.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26335..26982
                     /locus_tag="IWK70_000659"
     CDS             26335..26982
                     /locus_tag="IWK70_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9613228.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            27066..28010
                     /locus_tag="IWK70_000660"
     CDS             27066..28010
                     /locus_tag="IWK70_000660"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9613229.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28145..29596
                     /locus_tag="IWK70_000661"
     CDS             28145..29596
                     /locus_tag="IWK70_000661"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9613230.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29617..30453
                     /locus_tag="IWK70_000662"
     CDS             29617..30453
                     /locus_tag="IWK70_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9613231.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30453..31112
                     /locus_tag="IWK70_000663"
     CDS             30453..31112
                     /locus_tag="IWK70_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9613232.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31238..31927
                     /locus_tag="IWK70_000664"
     CDS             31238..31927
                     /locus_tag="IWK70_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613233.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31933..32889
                     /locus_tag="IWK70_000665"
     CDS             31933..32889
                     /locus_tag="IWK70_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9613234.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32933..33445
                     /locus_tag="IWK70_000666"
     CDS             32933..33445
                     /locus_tag="IWK70_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9613235.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33701..34249)
                     /locus_tag="IWK70_000667"
     CDS             complement(33701..34249)
                     /locus_tag="IWK70_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9613236.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34338..35519)
                     /locus_tag="IWK70_000668"
     CDS             complement(34338..35519)
                     /locus_tag="IWK70_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9613237.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35755..37263)
                     /locus_tag="IWK70_000669"
     CDS             complement(35755..37263)
                     /locus_tag="IWK70_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9613238.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37422..37613
                     /locus_tag="IWK70_000670"
     CDS             37422..37613
                     /locus_tag="IWK70_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613239.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37816..38733
                     /locus_tag="IWK70_000671"
     CDS             37816..38733
                     /locus_tag="IWK70_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9613240.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38726..39799
                     /locus_tag="IWK70_000672"
     CDS             38726..39799
                     /locus_tag="IWK70_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613241.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39947..40465
                     /locus_tag="IWK70_000673"
     CDS             39947..40465
                     /locus_tag="IWK70_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613242.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40446..40838
                     /locus_tag="IWK70_000674"
     CDS             40446..40838
                     /locus_tag="IWK70_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9613243.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40914..41849
                     /locus_tag="IWK70_000675"
     CDS             40914..41849
                     /locus_tag="IWK70_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9613244.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41982..42761
                     /locus_tag="IWK70_000676"
     CDS             41982..42761
                     /locus_tag="IWK70_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9613245.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42754..43071
                     /locus_tag="IWK70_000677"
     CDS             42754..43071
                     /locus_tag="IWK70_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9613246.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43315..44925
                     /locus_tag="IWK70_000678"
     CDS             43315..44925
                     /locus_tag="IWK70_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9613247.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45082..45930
                     /locus_tag="IWK70_000679"
     CDS             45082..45930
                     /locus_tag="IWK70_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9613248.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            46161..46640
                     /locus_tag="IWK70_000680"
     CDS             46161..46640
                     /locus_tag="IWK70_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613249.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            46673..48901
                     /locus_tag="IWK70_000681"
     CDS             46673..48901
                     /locus_tag="IWK70_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9613250.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            49030..50673
                     /locus_tag="IWK70_000682"
     CDS             49030..50673
                     /locus_tag="IWK70_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9613251.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(50746..51291)
                     /locus_tag="IWK70_000683"
     CDS             complement(50746..51291)
                     /locus_tag="IWK70_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9613252.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(51314..51799)
                     /locus_tag="IWK70_000684"
     CDS             complement(51314..51799)
                     /locus_tag="IWK70_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9613253.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(51789..52601)
                     /locus_tag="IWK70_000685"
     CDS             complement(51789..52601)
                     /locus_tag="IWK70_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9613254.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(52740..>53851)
                     /locus_tag="IWK70_000686"
     CDS             complement(52740..>53851)
                     /locus_tag="IWK70_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9613255.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        17936 a         9795 c        10401 g        15719 t
ORIGIN      
        1 attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg agtttatcca
       61 tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga aaagtatccc
      121 taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg aaaacggtcg
      181 ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa aactgatctc
      241 tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga taaatgcagc
      301 ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt tcaaatttga
      361 agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta atgaactgtt
      421 tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg tatatataaa
      481 caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat ttcgggaaga
      541 caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag ctatctctca
      601 gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga aattacttgg
      661 tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg atttcctcaa
      721 agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca agcaagagaa
      781 ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg cttatattgc
      841 cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc agcactatca
      901 agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc aagctttgaa
      961 caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa ataaaagtgc
     1021 attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg ctgatgtcaa
     1081 aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt acagtttttc
     1141 atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt ataaaggaac
     1201 attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg tttttccaga
     1261 aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag ggaatattat
     1321 agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag gagtcgttcc
     1381 aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg cttcctcctt
     1441 cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac aacccgatta
     1501 ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg gagctaccat
     1561 tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc tgattggtta
     1621 cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca gtaatggaaa
     1681 aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga caacaggtgg
     1741 gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg aacatgaagt
     1801 tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa cagcttttga
     1861 cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg gtgatcttct
     1921 tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga tctcacaaga
     1981 agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat ttgcgacagg
     2041 ttatgctcct ggatctacgt ttaaaataat tacagcagca atcggtctcg acaacggcac
     2101 tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag acagttcttg
     2161 gggatctttt caaacaacgc gtgtcagtag tgatgtatca caagtagact taaaaactgc
     2221 tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg gtgagaaaaa
     2281 atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc caattagtat
     2341 gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc tagctgatac
     2401 tggatatgga cagggcgaac ttctaattaa tcctatccag caagcagcaa tgtattctgt
     2461 ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag agacaaaaga
     2521 taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc tgagagaagt
     2581 tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc cgttggcagc
     2641 gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag agaacagttt
     2701 cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt tggaaaataa
     2761 agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc tcaaccaaaa
     2821 ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta tcgtcacagt
     2881 ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt tgatttactc
     2941 acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata agtgtactta
     3001 ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga ctttgctgtc
     3061 caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca ccgaccatca
     3121 atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca agcaatttgg
     3181 tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta aagtcagaaa
     3241 aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca tatcctaaag
     3301 cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct atcccagcaa
     3361 aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac attcctaaac
     3421 aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct gcaaatgaaa
     3481 gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa attagctgtt
     3541 caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga aaagataggc
     3601 tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc aaaattgttg
     3661 atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa atactgacag
     3721 aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact tggaatgctt
     3781 tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca acgaacatac
     3841 caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct aaaaacatta
     3901 ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg acccaattca
     3961 atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct gggataccta
     4021 atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat aaaataccaa
     4081 taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc ataacataca
     4141 ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa gatttttttg
     4201 gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt atagtcattt
     4261 ttcttaatct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa ataaagccga
     4321 atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg aatccgccaa
     4381 tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc atgctgaaga
     4441 acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc aggatagaaa
     4501 cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa agaccactga
     4561 acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca aaaagattca
     4621 acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga aaaacagcag
     4681 aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt aataaggcgt
     4741 atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt tgacttattt
     4801 taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag gagaagaata
     4861 tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg ctagctcatc
     4921 gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt cttctactcg
     4981 tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag gggcttccgc
     5041 aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg tttgggaata
     5101 acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt ttctctccgc
     5161 ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac gattttgcgc
     5221 cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata taagaaaaag
     5281 agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc ttgtcaccat
     5341 ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca tcagataatc
     5401 gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt gtttgtatgg
     5461 actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc catttttctc
     5521 ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat ccgtattagg
     5581 gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct ccatcgtacg
     5641 gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt tcgtcgctgc
     5701 atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga tttgatctgc
     5761 ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag ccaaatcgat
     5821 tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc ctttaaataa
     5881 agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt tttcgcctgg
     5941 tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg ctttaagtga
     6001 tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg aaacggcctg
     6061 ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt gctgcatcaa
     6121 ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg tttcaacgac
     6181 ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg ctgcatagat
     6241 agctgcacta ctagctgctg gtccaccgcc aattactaat acatcataag gttctttttc
     6301 aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt cgatcgtcat
     6361 gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca tgattttttt
     6421 atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa tatttggatt
     6481 taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac aagtcaagct
     6541 aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac tcaaagattc
     6601 actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag aggaaaattc
     6661 atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg gattgataga
     6721 aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag acatttctgc
     6781 tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca aactaacaga
     6841 aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt tagtttcttt
     6901 gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt tcaatgtttc
     6961 gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta catattgagc
     7021 agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg cattgatttc
     7081 catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag ctaatccttg
     7141 ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac cgatcatcac
     7201 gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag taaagtgggt
     7261 atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt attgttcttg
     7321 taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga agcaaagaat
     7381 actccagttc cccataagat ctttttcact gacttctaaa aattctcctt gatgataagc
     7441 atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac ctccaataat
     7501 aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg gatattttgt
     7561 tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga ggtcgcaaga
     7621 aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg ttctttcagc
     7681 atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt atcaactaaa
     7741 gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc ctgaatggtc
     7801 actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca gttccaaact
     7861 tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc acggaaaaaa
     7921 gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt ttctagcact
     7981 attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc ttccaactgt
     8041 tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac atacgtacca
     8101 ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc ttcgcgaatg
     8161 ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt ccttccaggt
     8221 tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga ttgcaagctg
     8281 cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag aagaggtctg
     8341 aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa aattttggtt
     8401 gacatagcga tttttattgt ataaaattca cttgaataca aaaactaata tgttagttta
     8461 ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa ttatctcaaa
     8521 aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga caaaaaaatc
     8581 ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca gaaatcgctc
     8641 aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa acattcgatg
     8701 aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa gtggtcatgc
     8761 caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt ctgtattgtc
     8821 atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat cctagtttac
     8881 aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca aataataaat
     8941 atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca catacaatga
     9001 gtattcttgc tgcgctactt tataaacgtt ttttatctgg agcttggccg attgcacttg
     9061 tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg ctaacgatcg
     9121 caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac ttatcgaatg
     9181 aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat ccttccttgt
     9241 cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc caaaccgcaa
     9301 aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta atcatggaag
     9361 atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg actgatcgtg
     9421 caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct ttgcatacgt
     9481 ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag atgaaagatc
     9541 cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca gttgtggagt
     9601 ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg cgcttttcta
     9661 atccaaatat tcctgatatg cctcagcgaa tcgccacgga tacctctcaa aaggtggcga
     9721 ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac ccgcagacac
     9781 ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc attgatgatc
     9841 aaggaaaacc attcatcccg agtccagacc cactattgac agactttcag cataagcttt
     9901 ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg gctcctattt
     9961 tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc agaaaaatag
    10021 aagaaatgtt catcaagatg gtagcaggac cgaatgccgt acgaaaaaca ttacaggatt
    10081 acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt acggatcaca
    10141 ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag ggattgtagg
    10201 cacattgatg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
    10261 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcccagggag
    10321 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
    10381 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
    10441 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
    10501 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
    10561 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
    10621 atcgaacatt ccagcaatga atttgaattg ccgggatggg aacctgagcg cttagctcat
    10681 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
    10741 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
    10801 catccagatg atccgccata ctcgattttt ggattacctc gaatcgtaaa gaatcgagaa
    10861 gatttagatt ggttgtgccg catagtagac agcccttata acggaatcac tctttgtaca
    10921 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgtcaa
    10981 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
    11041 tatgaggcac cgcataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
    11101 atgtatgaca acggatttaa cgaatacatc cgtcctgatc atggaagaat gatctggaat
    11161 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
    11221 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
    11281 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
    11341 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
    11401 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
    11461 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
    11521 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
    11581 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
    11641 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
    11701 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
    11761 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
    11821 gtttggactt ctttcaacac gctatgttct tgataaagca tgaaagtagg aaggggcgta
    11881 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
    11941 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaaa ggactccacc
    12001 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
    12061 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
    12121 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gggatcaata
    12181 aaaaagtcaa tatattcata ctctgtgaat tctttttgta cgtctttcac agaagttgca
    12241 ggaaaattct ctttacatat ttttaataga aaatataatt tatctatcat accgctattt
    12301 agtcggtcaa gtacttcttt tttaaaaaaa gtattcatca taaaagccac tacttgcgga
    12361 tcttcatcat aaaattctgt agcagtactt gaaaagatgt tccaataaat atctaatgct
    12421 gcatattgaa tttgtacctc ttttccagaa aaagactttc tgccattatt agaaaaggag
    12481 atagacaaac cgtacaatgc taaaaatgca ttaatctttt tcattttcgt atacaaataa
    12541 gaggcactat agtttgtttg ctgacttaaa ctgataatcg aatttgtctg acagctgaac
    12601 aaagaagtca agatctgata actgactgat tcgttgcagt atcttccaaa tagacgataa
    12661 tagcagtaag ccggatcaat tccttcaaaa ttcaacttta aaaaaggttt ttgcttatca
    12721 atatgcagtt ctgttagatt gagacgctca aagtcttgtt ctaattcttt gatgtatagt
    12781 aaaaaatttg attcagacat atggaaatga tctgctaaat attgtgtagt aataaagaaa
    12841 cgatgttcag ataggaaaag aaaaatatct aattttgtat tgcttttttt cttcaagaga
    12901 aacgttttaa tcgcttcttg agtttgcatg aaagaggctc ctttttactt aaattgttaa
    12961 aacttgaaaa tattactgtt tttttcataa atttagacaa aaaaacagta aacatatagg
    13021 tagaatctct actatcttat ttcttgcata caaaaaaatc aaggagtgag agagttatga
    13081 gatattcaac catcttaata tcaacatttg cagccttaag cttatcgctt actataaatc
    13141 agacagtcca cacagaggag atagaaacag ccaaatggac agaaaccaag tgggccaacg
    13201 tcaatcgaat tacctttcag gatgaatttt catccttcaa ttcaggagtc gcaaaattaa
    13261 gaaaaggaca aacagcgacc atcaaagcaa aaattgacta ttcaggagac ttagcaccta
    13321 tactacaatc agctgttaca tttgagaatg ttgatcccgc tttgtcgatt ggctatgacg
    13381 agaacacttt gactatccaa cacaagcaag cgatatttac cttcaccgtt acattaaatc
    13441 aggacttgtt aaaaccagcg ctttttactg tcaaggtatc agatggacta ccgaacgatc
    13501 atcatcattt aacgccgtat agtcagcggc agacaattga gcaagattca gctatcgact
    13561 caggaggaga tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag
    13621 taccgcctac agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac
    13681 ccgatacaga aactcaacca gagccagaca atgaatcaaa acctgaaata acccctggag
    13741 aaaaaccaga tgtagaccca gaagaaaaac cagatgtgac tccagaacca gatacggatt
    13801 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
    13861 aaaaaccaga ggtagcccca gaagaaaaac cagatgtaac tccagaacca gatacggatt
    13921 ctggaaacca aacagtaccg gaaacaaatc cagacacaga taatgaaact gaaaatccag
    13981 aaaaaccaga ggtagatcca gaagaaaaac cagatgtgac tccagaacca gatacggacg
    14041 caagagacca aggaatacca gaaaaaataa acaaaaaaac aattcaagag gatggaaaaa
    14101 aagagagtaa aaaatcaaat ctagcaattc taaaaataaa tgaagagcag ctgaataaaa
    14161 aatcacgaat atttgatagc gcccaatcag ctgaaacctt aaaaagtagc aaggatacaa
    14221 cctttgctgt cctgaaacga aaaataaaca gctaccaaaa tccggagagt cacaaaacaa
    14281 agttatactg tggagcggaa tcattctatt aagtatagca acaatgcttt ctgcaaaaag
    14341 atttaaacaa aacagatctc tctaacattt gaataatgtc tgtgaacgca tttttcaaat
    14401 aaaaagaaat atccagaaaa tgaccccaaa ataccagatt gaaatctgat atttggggtc
    14461 gttcatactt tatctttctt catatttatt ttaagggata tctgtttctt ttggaagatg
    14521 gttcgggatc tttttcactt tcaatacatc taaaaagaag gtaaagatcg gaatgccgac
    14581 aatcaagccc caaacgccaa agaaccgttc gctaacaagt aagaccacaa atgtataaaa
    14641 aatcggcagt tctgttttgc tggacatcaa cttcggattc agcacataag actcaattag
    14701 atggacaatc aaaatcgtaa tcaggatata aacaacatct cggatgcctc cttgggaata
    14761 agctatgaaa gatagaggaa tacaagaaat gataacacct gctactggga tcagacttaa
    14821 aatgaaaatc atgatcgcta aactcagcag ctgatgaaag ccaaatacag ccaaggcgat
    14881 tgtagtgagc aacgtattta aaagagcgac aacaaattga gcttctaata ccaaaccaaa
    14941 tgtattgaca aacttatcgg caaaatagta aatatcttga aagaaccaag aaaattcact
    15001 ttttaaaaac aatcttgaga atacaattgt ttttttcttt tcaatcataa aaaagaaact
    15061 taagatgaat gacatagcaa acgccactgc catttttcct atatcctgta aatagccaag
    15121 cgcaatcgaa gcgccatttt gaagctgttc aaaaaaatcg tttcgctcaa tataagtatg
    15181 gatatattgt aaaacaggat ctgcatcttt tggctgatgc tcgtaaaaat taataacgga
    15241 ctggatcatg tcaaatgttt gatgaataag catcgggata tactttgtga atgccagata
    15301 gataaggtaa acgatcagtg tataaatcat gatagctaaa acacctgtag ggattcggac
    15361 aaatcgctga atgatttgaa caagatgaat catcaaataa gtaaagataa atgtgagcaa
    15421 aatggttgta atcatacttc taactaagaa aagaacaaaa acgacaaata ataaaacagt
    15481 gagacggcgt attttttcat tcgccaaaaa acgctgataa acggtcaaat cgacaacctc
    15541 caaataacaa aaaataaaca cgttattcat tatatgatgg aatatgaaaa taagaaagag
    15601 caaaaagaaa aaccgctaaa tgacgttatc ttatgagttg gttttcctca taaaatcgtt
    15661 catctagcag tttcagccct ttttctggtc ttgatgaaaa ttatctagca tttttcgggt
    15721 cagatgttgc agtgtatcca attcttcctg ttcaagtgga agataagact tgatcttttg
    15781 tggaatacct tctgcttgtt tttgcaacgc agcaccttga gcagtcagcg taacatttac
    15841 gattctttca tcacccgttt cacgctgacg attaatataa tttttttctg ctaacttttt
    15901 tagtaaggga gtcagcgtac cagaatccag acacaaatca tttcctagct ctttcactga
    15961 agtctttttc ttttcccata aaactaacat cacaagatac tgggtatagg taagattcag
    16021 cggcttgagg aggggagtgt agtgtctaat gatttcttta gaggcggcat ataaaggaaa
    16081 acatacttga ttaactaaat atagatcttc catttttcca cctccgctgt tttactatag
    16141 tgtatcatag cggatttaca caaaaaagtc tattcactaa ctgctttcag agtaacttct
    16201 atatttccag ctgtagcttt tgaatacgga cacactttat gcgctgcctc taataattct
    16261 tgcgtttctt ccagagaaag accttcgata gatccttcaa tctctacacc taagacaaag
    16321 ctcgtttcac tttctgaata aagggaaact tgtgcactga cagtagaagc cccagagata
    16381 tttttttgct gtttgatcaa ttctaatgca ctgttaaaac atgcactgaa accagctgca
    16441 aacaattgtt cagggttggt tgcattttcg acgcgtttac caggagctgc aatattcaat
    16501 tcgaatgtac gatcgggatt atgcacttct ccagagcgtc cgcctgtgtt gatcattttc
    16561 gtagaataca tttttttcat aaaattacca cctttaatta tattttatac aattgaattt
    16621 tacacaatat aattgtggaa agcaaagaaa tggattggca taagataaat aagagaaagc
    16681 acatgaaaca gacgcgcttt ctcgaatagc aaacatatac aaaaaatatc agagacaaaa
    16741 aataatctca tcataaacat tacatatatt tctcaaaaag ttgttttctt tcttcaaaaa
    16801 ttacttcacg atggacctga tgaagcatcc ataaataacc aaaaggatct ctgaacattg
    16861 agttagtcac accaaagtcc atcatttctg tcatttcttg aatctctaca cagccatttc
    16921 gtaatgcttt attatgtgtg tcctcgatat cagagacgat gatattgaac cagatcgtat
    16981 ttggatgttc agcagtagga gcagtcaaac caagagacgt gttttcatcc agcatatgga
    17041 atctcgtacc atacaaggta aaaatgactt cattattgcc tctagggtaa tctgttgcct
    17101 caatcagttg tatttcatca aagattgttt gatacagttc tagtgctttc aaactatcat
    17161 ttaatacaaa atcgatttcc gttcctttat gcataaagat ctcctctcct tttcagtcga
    17221 acagttacta gattctttta ttataccagc taaagaaaac gcattcaaaa aaatagcgga
    17281 tttacttacc aactcattcg attggatgat aagtaaatcc gcgtcccatt acttctgcta
    17341 cgctgctgac aatgacaaag gcacttggat cgatttcttc tatcaatggt ttcattcggg
    17401 aaaattcatg atcagtcaca acgatcaata aaacctcttt tgactttcga ttatacccgc
    17461 cttttgcctc tagcagggta aggccacgtc caatctcagc acttaagcga aggcggatgt
    17521 cttccaaata atgttcactc atgaccataa cagatttctt tcgattgata cctgtttcga
    17581 tataagtcat gacaattgaa gtgattgcaa tagataaaat agcataaaga aactcttcta
    17641 ttccaaaaat aaacagatta agagaaacaa cgaatgcgtc tgtaaaaaat aagccaatcg
    17701 aagtattcaa gtggaaatat tttttaaaaa tgaggggagg gatcgtcgta ccgccgctag
    17761 aagagttgtt tttatataaa atcgcaactc ctaaagcaaa aatcgcactt ccaaaaatga
    17821 cagacaacaa ccgatctgaa gtaatcattg tttctggaac tattgcaatg attaaaggaa
    17881 aaaccaagct tccgaaaagt actttaaaaa agggtttttt acctaaaaat agaagagcta
    17941 gtaccaacat aacgatattc aacactaaaa taactatagc tcggtcaaag cctaaagcag
    18001 cttccagtaa tattcctaga ccgctgacac ctccagcagc aatatgatga ggccctaaaa
    18061 acatattgat agcgactcct aatatgaaga gggcaaaaat aatcttaatg tatgtccata
    18121 taaattgtct cgtttctttc atttattcac ttccttaatt cattaataat actcttatct
    18181 tattataact tacttttcac cttaagttaa ctttaattaa taaaaaaatt aggctagtct
    18241 aaattattat gtttcattct tctagaatat acttctacgc caaaaactgt taaaaaactt
    18301 ttactttaga aaattggaga aattgatcgc ttaagataaa ttcaggacac taaatagtac
    18361 aggaagtaga ctaaaacttt taaaagtttt aagtctatct atttggcaag tttaaaaaat
    18421 aaaaattcgg tatcaaaaaa ctgttaccga taacattttt gaaagcgttg tcttttgctt
    18481 ctctatcact tatagtgatg acgtcatatt aaaacacaag gaggaaatca atggatacag
    18541 ctgcaattta tcatcgaccg gaaagtgaat atgcatttct atatacgaat acaagatttc
    18601 gtattcggct tcgtactagg aaaaatgata tcaaaaaagt atatgtgcta tgtggagatc
    18661 catataccat aacaactgaa aaatggtatc aaaaacaacg accaatgaaa aaatgtctaa
    18721 gtaccaatgt tcatgattat tgggaaattg aagtgacgag cgaaacaagt cgcttacagt
    18781 atgcgttcca tgttgttggg gaagataaca cggactgttt ttacggtgat caaggaattt
    18841 ttccttatcg agaaaatgtg attactgaac caaattttta ttttcgaata ccttattttc
    18901 atcaaatcga tcgattcact attccagaat gggtaaaaga aacagtatgg tatcaaattt
    18961 ttcccgaaag atttgcaaac ggggacaaag caaatgaccc cgaacacact ttaccttggg
    19021 gaagtaaaga tccagaccga gaagactttt tcgggggaga tttgcaaggt gtgatggatc
    19081 atttggatta cttgacggac cttggcatca atggcatcta tttttgtccc atatttaaag
    19141 ccacatccaa tcataaatac gatacgattg attattatga ggtggatccc gcttttggcg
    19201 ataaaaaact tttaaaaaac ttgattgatg aagcacataa aagagggata cgtatcatgt
    19261 tcgatgctgt attcaatcat atgggtgtcc attcaccaca gtggcaagac gttctcaaaa
    19321 acggagaaaa atcgatctat aaagactggt tccacatccg ttttttccca gtagattctt
    19381 atcagatgac tgacttacca gaaactgctg aaaatatccc atatgatact tttgctttta
    19441 ctccattcat gccgaaacta aatacagcaa atccagaagt gcaaaaatat ttgctggata
    19501 tcgcaactta ttggataaaa gaattcgata ttgatggatg gcgtcttgat gttgctaatg
    19561 aagtcgatca tcatttttgg aaaaaattca gagaagcagt tacagaaatc aaaccagaca
    19621 tctatatcct tggagagatt tggcattcct ctcaagcttg gctgcaaggg gatgaattcc
    19681 atgcagtgat gaattatgca tttacagatt cgattaaaga ttattttgca aaaaagaaaa
    19741 tcacagcttc acaaatggtc agtggaatga atcatcagca aatgctttac agagatcagg
    19801 tgaacgaagg aacattcaac ttattagatt ctcatgatac tgccaggatc ttgacccttt
    19861 gccaaggtaa caaagaattg atgaagtctg tgatggcctt tatgtttctt caaaaagggt
    19921 ctccttgtat ctattacggg acagaaatag ggatgactgg tgaagatgac ccggattgcc
    19981 gcaaatgtat gatatgggag aaggaggaac aagatctaga gctattcggt tttataaaag
    20041 aacttgtttc tctaaggaaa cagttgtcta agattatttc tgaaggttct actcagtggc
    20101 taatagtaaa tgatcgtgaa gataagctct atttcacacg gaaactagag ggacagatca
    20161 tctatgttta ctttaatcag tcaaaagaac cttgggtagt agaacaggaa aatgaggtga
    20221 ttcttagtca aaattgtcag ttgttagaag acgggaaagc agagatacga caatacggat
    20281 ttttgatcat gtgttgaaaa ctgttaccgg taacaacaag aaagcgtttg caaatgaaga
    20341 tttgtgccca ttataataag ggtgtaacag cacgttgaat acaaaggagg aaatgaagat
    20401 gaaaaaaaga agtgtgaaac agttgggttt agggttaatc tctttaggaa tcttaggcgg
    20461 attagcggct tgtggcaatg gtaactcttc agcagaaggg gaagccgatg aaggaaaaac
    20521 gttaacagtt tccgttgatt ctggatataa agattatata aacgaaataa aaaacacttt
    20581 cgaaaaagaa aacgatgtga aaatcaagct agtagaaaag gacatgttcg atcagttgga
    20641 atcattagcg ttagatgggc ctgctggaaa gggacctgac gttatgatgg cagcatatga
    20701 ccggatcggt gcgttaggac agcaaggaca tctggcggaa gtaaaactgg gaaatgaagc
    20761 agcttacgat gaaacagaca aagcccaagt gacttatgat ggaaaaatct acggagaacc
    20821 tgcagtaatc gagacccttg ttttgtacta taacaaagat ttagtcgata cagcaccatc
    20881 aacgtttaaa gatttggaga atctatccaa agacagtcgt tttgcttttg agtcagaagc
    20941 aggaaaaaat acaggatttt tagctaaatg gactgacttt tattattcct atggtttaat
    21001 tgcaggttac ggtggctatg tatttggtga tgatggcaca aatccttcag atatcggttt
    21061 gaataatgca ggtgctgtag aaggaatctc ttatgcaaca gattggttca aaaatgtttg
    21121 gccaaaggga atgcaagaca taaaaagtgc aggagacttc gccagccagc aattcatgtc
    21181 caataaaact gcagcaatca ttgacggtcc atggcaagcg caaacataca aagaaaacaa
    21241 tatcaattat ggtgtagcca aaattcctac tctaaataat ggacagcctt atcaaccgtt
    21301 tggcggagga aaaggatggg tagtcagcaa ttatgctaaa aacaaagacc tctctcaaaa
    21361 atggcttgat tatgtaacga atcaggaaaa tcaagaaaaa ttctttgaaa tggtaaatga
    21421 aatccctgca aaccaacaag ctagggaaac agcaaaggct aaaaacgatg aattaacaac
    21481 agctgtgatt gaacaatatg aaacagctca agcgatgcca aacatcccag aaatgggcga
    21541 agtttggaca ggtgcagaaa acttgatgtt cgatgcagca tcaggttcaa aaacaccaaa
    21601 agaatccgca gatgaagcag taaaaacaat cagtgaagcc atagaacaaa aatactgatt
    21661 aaaatgataa ccgtccattt ttataaaaaa tggaacaggt gcttcagata aaagtggcaa
    21721 gagcgagatg gaaaattatc ttgctcttgt tgccattatc ttaaaagcag tttgcggttt
    21781 agcaagttta gagggggcat tcagatgcag caagataaaa caagcaatat caacgaaaaa
    21841 aaaatcacgc tttgttcact tattccaggg atggggcagt ttgtaaatgg ccaagctttt
    21901 aaaggtatcg tttttttcgc catattcgtc ctattcattg gtcagatgct tctgggcgga
    21961 tttcatgcat tggtaggatt ggttactttg ggaagtatac caatggaaga tcattcatta
    22021 tttattttga ttgaaggcac tttgcaattc atcattacaa tcattttctt gattttttac
    22081 ggtttgaatc ttttagacgc ttatcgagta gcgaagatgc gtcaagaggg caaaaaaatc
    22141 aatcggacga tgaaggaagt catacataat ataggtgatg aaggttttcc ttatctattg
    22201 acacttcccg cttatcttct catgattttt acgatcattt tcccagtcct agttacattg
    22261 ttcactgcat ttactaatta tgacttcaat catattccac cagccagttt aattgattgg
    22321 gtaggggcta aaaacttttt tagtatcttc tttttaagct catatcgtaa tacttttttt
    22381 gcagtctttt cgtggaccgt gatttggact ttatgtgcaa caactttgca aatcaccctt
    22441 ggtattctca cagcggttgt tgctcaccag aaatttatta aattcaaacg tattttcggt
    22501 atcattttct tattaccatg ggcggtccca gcttttatta ctatcatgag tttttcgaat
    22561 atattcaacg atagcgtagg tgccatcaat actcaagtga ttcctttcat aaaccagctt
    22621 ccttttgtag atatcagtgc aatctcttgg aaaacggacc cgaactggac gaaagtagca
    22681 attatcttag tgcagggctg gttaggattt ccctatatct atgtgatggt tacaggaatc
    22741 ttacaatcga tttcagaaga cttatatgaa gcagcaaaaa tcgacggagc aaatgcatgg
    22801 cagcgctttt caaacatcac gcttcctgct gtcttcctag tggcagcgcc aacatttatt
    22861 acacagtata cggggaattt caataatttc tccatgatct atctgtttaa caatggcggt
    22921 cccggaagcg taggcggagg agctggttct actgatattc ttatttcctg gatctataaa
    22981 cttacgactg gaacttctcc gcagtattct atggcttcag caatcacgtt gattatctcc
    23041 gtcatcgtta tctgtgtgtc attgctggta tttaaaaaga caaatgcgtt caatatgggg
    23101 gattaacaat gaaaaaattt cattcgagtg ctcgattcca aaaacgactt tctcactttt
    23161 ttacttactt ttttctttta atactgtcag tcattatcat ctatccgctt ttgatcacag
    23221 caagtactgc cttcaagtca ggaaatattg cagcttttag tttagatctt tcaagtgact
    23281 ggactttagg aaactttact cggctattca gtgaaacact ttacgggact tggtataaaa
    23341 atacattgat catttctgtc ctaacaatga tcgtacaagt gactgtagta actttggcgg
    23401 gctatactta tagtcgctat cgttttatcg gccgcaaatc aagtttgatg ttctttttag
    23461 tgattcagat ggtaccgact atggcagcat tgactgcctt ttatgtcatg gctttcttat
    23521 tagaagcgct cgataagtac tggttcttga caatgatcta tataggcggt ggaattccaa
    23581 tgaatacttg gctgatgaaa ggatactttg acactgtacc aatagattta gatgaatctg
    23641 ctaaacttga tggggcaggg catttcagga tttttttcca gattgtatta ccgcttgtca
    23701 aaccgatgct cgccgttcaa gcattatggg cttttatggg accttttggg gatttcatgt
    23761 tagctaagtt cctgcttcga acacctgaaa atcagactgt tgctgtcggc ttacaaacat
    23821 ttatttctga tgtgacaaac caaagagtct ctctttttgc tgctggggca attttgattg
    23881 cactaccgat ttgtctatta ttcttctttt tacaaaagaa ctttgttacc ggcctgacat
    23941 ctggcggaac aaaaggatag gagcagagta tcatgcaaac atttccgtta aactattttt
    24001 ccgcattacg tacgccaact caagtgtttg cgggacgcaa actattatca tggcctaaat
    24061 ttttcttgat atttgtattt ttagtttcct tgatggtcat gcctgtgact cttttttatg
    24121 ccaatcagat ccaagcgatt ccattagaac aatttctatc cgttcattcg ctgatcgatg
    24181 aacaaggaac ccaaaaattt tctgaacttg aattatctga aactgggtta caagcatcgc
    24241 aacaaacgat agctgtgacc cctgaaattt tggtaggtgt gtctttatct gagaaacaac
    24301 aatccgatca tggaactttt attgattttg agaaggaaca atgggtgatc caacaaaagg
    24361 ataaatctgg tatacgccgc tatacgatga attatagccc atctttccaa ccagattctg
    24421 tacgtacgcc cgaagatttc caacgatttt tagaaagaga attttatgcc agtaatcgtc
    24481 caacaatcat tttatcttat agtttaagtc ttggtcttgt cctatttgtc atgacgagtc
    24541 tcattttatt cggggcttcc tttttcttgt ggatgacaag aaaaagccaa ctttcatcca
    24601 ttcatacatt caaagagagt gcaaatttga tgctgaatgt tatgggaata ggttccatgg
    24661 cagcagcagt tgtagggttt atccactttg actttatctt gatgctaagt gtccaaacaa
    24721 cgataactgt cttgctgctt ttatgggttt ttgtcaaaac aagattcaaa gataagcgtg
    24781 tcgaatgaca agtttaagcg atttatgcga caatgaatgt gaaaaaaatg aaaaataaag
    24841 tagtttttga aaaatttctg aaaaaattaa acttcttagt tgattctttt cttttttagt
    24901 attaacgtaa tagggtagtt tttttaagga ggaaaatcat gttagagaaa actttataca
    24961 atcaattatt ttcacgttct ttctcgctcc ctgtcgaagt aaattattgg gacggtacaa
    25021 cgaaacaata cggagatacg gacactcctc cccaaatcaa aatcaatatc catgaagaaa
    25081 tcccaatgaa agagattaca agtaacgctt ctttggcact aggagaagcc tatatggatc
    25141 atcgaatcga gatcgaggga agtatccaag cattgatcaa cgatgcttat cagcaagcaa
    25201 acagcttttt acgaggaaaa gattatctaa aatggctgcc aaagaaagaa aaacatacga
    25261 aacaaaaaaa caaagaagat atccatgcgc attatgactt aggtaacgac ttttataaac
    25321 tttggctaga tccaactttg acttattctt gtgcttattt caatacacct gaagatacgt
    25381 tggaacaagc acaaatcaat aaagtccatc atatcttaga caaactcttt atcaaagaag
    25441 gcgaaacgtt gcttgatatc ggttgtggct ggggaacatt gatgttcact gctaccaaag
    25501 agtataatgt caaggcaact gggatcacac taagtgaaga acaatacgat ttgattacca
    25561 aaagaataaa agaagaacat ttagaagata aatgccgtgt attattaatg gattaccgcg
    25621 aactaaaagg cgaaacgttt gatcatatca caagtgtagg tatgttcgaa catgtaggtt
    25681 ctgaaaatct tgaaggttat ttcaaagtgg taaaagattt attgaaacca aaaggaacag
    25741 ctttgatcca tggaatcagc cgacaacaag gcggtgcgac aaatgcttgg atcaataaat
    25801 atattttccc tggcggttat gtccctggtg ttactgaatt agttgataat attacaaaaa
    25861 acgatttgca actgattgat ttggaaagct tacgtcgtga ctaccaattg acattagaac
    25921 attggacaaa gaattttcat caagtccaag atcaagtcac gaaagaaatg ggtgattcct
    25981 tctatcgaat gtgggatcta tatttacaag cttgtgcagc ttcatttgaa tcaagtaata
    26041 tcgatgtgat tcaatatttg cttgttcaac ctggaaacaa tgacatccca atgcatcgtg
    26101 cataattgag gcaaaagtac tgtttaaaga ctatttaaaa agaagctaag ttttatgatg
    26161 ctccagaagt gaactttttt gtctaacttc taggattgtc tctaaagctt agtttctttt
    26221 tctttatatt ttcataataa aagtttggtt ctttaatttt taaacatgac agaaaatttt
    26281 ttcttcgtta tacttgtaat agaatactat tttataacaa aggggatcga atcaatgaaa
    26341 aaaactgtta tttatactag tttatttgca gctttgtttt tagctgcttg tcaatcaaat
    26401 accaatcaag gcacatctgg gaatacgtct tccagtagcg aacagcgctc ttcacaggta
    26461 tcggactcct caacaaaagc aagcacatct tcgtctagcc agacaaaaag ttctagtgaa
    26521 gcaagtactc agtcagatat caaagtgtct gcagctcgtg cgatcgaatt gttccaagat
    26581 aaatacccag atactgcaat tactagtttg gaattagata gtgattgggg atcttacttc
    26641 tacaagatag aaggtgtcga tgatcaaaaa gaatacgaag caagaattga tgctcaaaat
    26701 gaaaaaatgg agacagagaa tccagaaact ctagatcgag atgaacaaaa tggtcaaaag
    26761 aaaaaagaag atggtctgga tgttgataaa ttgatcacga ttgaggaagc agcaaaaatc
    26821 gccgtcgaca aagtaggaag cggtacggca acagattggg atttggataa agatttaggt
    26881 actacttatt gggaagtcaa agtaaaagac ggcaatcaaa gtacatcagt caagatcaat
    26941 agccaaacag gcgaagtact gtcaactgaa caagatgatt aagaatttct tcggaaaaca
    27001 agagttgatt tttcttttag tcatgataca ctagacaaaa taacttgttt gaaggagcga
    27061 tttacttgag tttagaaacg acctttgcca atcatatctt tgccaatcct ttgatgaatg
    27121 catctggtgt ccactgcatg actacccagg aattagatga actggctcat tcagaagcag
    27181 gagcatttat cactaagagc tgtacgatca atgaacgaaa aggaaatccc gaaccacgct
    27241 attttgatgt tcctttaggg agtatcaatt ctatgggact tccaaactta ggtttttctt
    27301 attatctaga gtatgctctt gcgtatgaaa aagtgcagga aaaccaaaat caaccgttat
    27361 ttttttccat tgccgggatg agtgtccagg aaaacttgga aatgcttgag aaaatagaaa
    27421 aaagtgggtt taatgggatc actgaattaa atttatcttg tcccaatgtg cctggaaaac
    27481 cacaactagc ttacgatttt gaggccactt atgaaacact gaaagaagta ttcagcatct
    27541 tttctaagcc gctaggaatc aaattgcctc cttattttga ttttgctcat tttgatcaga
    27601 tggctgatat cttgaatcag tttccattga cctatgtcaa tgctatcaac agtgtgggca
    27661 atggtttgta tatcgataca gaacaagaag cagtagtcat caaaccaaaa gaagggttcg
    27721 gcggcattgg cggagagtat atcaagccga ctgctttggc gaatgttcgc gctttttata
    27781 cacgtttgaa accagaaatc caaatcattg gtacaggtgg gattcggaca ggacaagatg
    27841 catttgaaca tctgctatgt ggcgcaagca tgttacagat tggtaccgag cttcataaag
    27901 aagggccgga aattttttct agaatcataa aagaattaac gcagattatg tcagaaaaag
    27961 gatatacgtc aattgacgaa tttaaaggca aattacgcac catttcatag caaagagacg
    28021 aaggtcatga ttgatcttcg tcttttttta taaaaaacac tcgtctgttc attcctgtgc
    28081 aagcgtactc attgtatgtt agacgaactt gtgggaaaat aacagaggca aaaaggagga
    28141 taatatgaat gatgcaacag aattagcaaa agagcttaaa gaaaaagaat ttagttttca
    28201 agaactagtt acggatatac agaaaaaaat aaagaagcaa aatcatgaat tgaatgcgtt
    28261 tgtcgcgctt gaaccattcg agagtaattc ccacgagcta ttgattcccc agtcagaaaa
    28321 agggccattg gctggtatcc catttccttt aaaaatgctc ggtcaagaaa aaaagggatg
    28381 gctagctact tcaggatctc gtttatttga aacacaccga gcttctcata catctaatta
    28441 tgtgcggcaa gcagaagcga taggtctagt tccttttgga caaaccaatg cgccagagtt
    28501 tggtttcaaa aatattacag acccagtcat ctatggccca gcaagaaacc cttggaacct
    28561 tgaatgtaca ccgggcggat caagtggagg agctgctgct gcagtttcat caggaatcgt
    28621 tcctttagct ggtgctagtg atggcggtgg ttctattaga atacccgctt cgttttgcgg
    28681 attgattggg ctgaaacctt cgcgagggac gatgccagtt ggtccatatg cttggagagg
    28741 atggcaagga gctgctattg atttcggctt gacagtttct atgagagaca cggaggcttt
    28801 attttatggg atgagaagta tccattccgg tgcgccatat caagtatcgc cagttgagtg
    28861 gcaaacacat ccaagaaaaa aacggttgaa gatagctttt tgtgttgatt caccgatcca
    28921 ctcacagatt agtgaagagg cagtacttgc tgtaaaaaaa gctgtagatt tccttggaaa
    28981 agaagggcat gatttgacag agattcctta cccactagat ggtcgtaaac tgatcacgtc
    29041 ttattatcag atgaatggcg cagaaacagc agcaatgatc cgctcgattg aaacaggcat
    29101 ccagcgagct gtcagaaaag aagaacttga acccatttct tggactttgt tccaatatgg
    29161 tcagaagctc tctgccgcta cttacatcga ttctctgcat gtatgggatg aagcagcaat
    29221 tgtcatggaa aacctacatc aagaatacga tctattcctt tcaccaacga ctgcatttac
    29281 cgcccctaaa atcagcgaag atttgcaaag tgatgcgata agagcacaga tggaacacat
    29341 cagtgattgt tctgaagtag aaggtttaga cgttgtttct tccatgtttg aggagagctt
    29401 gaagatcaca ccttatacac agttggctaa tctgactgga caaccagcaa tcagcttgcc
    29461 tactcatttg acaaaagaaa acatgcctct aggaatccaa tttatggctg ccagaggaag
    29521 agaagatttg ctatttcaaa tcggctatct atttgaagca aatcatcagt tccatttacc
    29581 aaatccatgg aaatagcaga aacaaggaga agaagaatga agaaaaaaag attgtttgct
    29641 ctaataacta gtgccttttt tattttaagc gcttgcggta cagctgccgc taatcaggaa
    29701 gataacagct ggagtaatgt agaaaaaaag aaagaattag tagtagcgac ttctggcaca
    29761 ctgtttcccg catcttatta taatgatcaa aatgaattag ttggttatga tgtagatttg
    29821 gcaaaagagg tggctaaacg gttaaatgtc aaaatctcct ttaaagaata caacgtggat
    29881 ggacaaatca cttctgtttc aaaaagagaa tcagattttg ctgcgaacga ttttggtatc
    29941 acaaaagaaa gagaaaaaaa attctccctt tcagagactt tcaaatattc atttgacagt
    30001 atgatcgtac gaaaaaaaga tgattcagga atccactcac ttgaagatct taaagggaaa
    30061 aaagcagctg gcgaacccaa tactagctat atgcgtttag cggaaaaaca cggagctaaa
    30121 ttagtcacat atgacaatgc cacgaatgat cagtatttaa gcgatgtagc aaatggtcga
    30181 acagatgtta ttctaaatga ttattatctg cagaaaatgg ccgtatctgc tttaccagat
    30241 atccctgtaa aaatactgga gaacgtttat ttcaatgaaa acgaaagtgg ttttttattg
    30301 aaagaaggaa atgatgctct gaaaaagaaa atcgatggcg tattaaaaga gatgaaagaa
    30361 gatgggacga caaagaaaat atctgaaaaa tattttcaaa ctgatgtttc agtcaaaccg
    30421 aaagaaacga tggctgagtc ggtgagtgag tgatgtactt tcctctaatt gatttttccc
    30481 tcatgtggac ttcacttcct ttcgtactgc aaggcttggt atacacatta gggattggct
    30541 tcgtgtcgtt tcttttagga aatctggtag ggttgctttt gacagtttta ggtcttttgg
    30601 attggctgcc tctgaatgta tttattcgtt tttatttatc gttctttaga ggcattcccg
    30661 cactagtcct gctgtttctg ttatattttg ggttacctta tcaattaagt gccttaacgg
    30721 cttcagtcat ctgctttacg attaccagca gtgcatttat tggtgaaatc tatcgaggat
    30781 cactagcagg cgtttcttca ggtcagtggg acgctggttt agcttctggt ttttctttct
    30841 ttggggtatt gaaatatatc gttttgccgc aagcttttcg aatctcagta ccggcactca
    30901 gtaacgtagc tatggatcta gtgaaaggta cttcgttagc agcaacaatc acagtgccag
    30961 aaatatttca aaaagcaaag atcataggcg gtcggacatt cgattatttt tctctctata
    31021 ttttagttgc actgatttat tgggtcattt gtatcgggat cgaaaaagtg caacagtatt
    31081 tagaaaaacg ttctatgcat cagtcaatct gaaaaataga aatggtgcta gcgtggctgg
    31141 caccatttct attttttgtt tcttgtatat tctgcctagt attttaaaac catttcatgt
    31201 aaaatgtaaa tcgaaaatat gttcggaggt agaaaacatg aagaccccgc taaccatcga
    31261 aatagaatcc tcgttatacc aatattgcat cgagcaaggc ggcttggttg tagaagaagt
    31321 cacgatgcct gatgaccaag gaattgtaga tacgcttagc tgcttgacaa aatttgatgg
    31381 aagccgagaa tggcgatgct atgagctgaa agtgacaaaa gcagattttc gctctacagc
    31441 caaactgtca tttgttggac attataatta ttttgtttta ccaagtaaac tatatgaaga
    31501 agtaaaaact gaaatcccta caggaatagg tgttttagtg tatcgtccgt acacggttga
    31561 agaggagatg cctgcctctg gtacatttct gattgcgaaa aaagcagcaa agaaagagct
    31621 tgctgtaccg gaagaagctt tgacaaaccg cttcatggct tctttattca gggaagtaag
    31681 aaaagcaaag caaatggagc gaggcgtaaa atattttcca acagatcaat tgtacaaaga
    31741 attgaaaaga cgttcagaaa atcaagatcc attttctaac gaacgatatt atgaacggtt
    31801 tttagaggaa atagctagtc aggcaatcaa tgagttgcaa gaagaagtta tcgcgcttca
    31861 gcatgattat gaatatttga ggcaacaaca gcagatccgt cggttgccta cagagccatt
    31921 ggagtgaaaa atatgtacta tccttattta agaggaaaac aatttgacct attagcatta
    31981 aaagaagcac tgtcgcgagg attgctctcc aataaaatcc aaccagtgat cgaaccagta
    32041 cgtgattccg ctacgcttaa aaatgtgatc gaattatttc aaaaaaagca ccatccaatc
    32101 gctgtgatcc aaaatcccca agtaggacaa ttcaaattat tcgatcagca tgtgcacagt
    32161 tggacagtca aagaaaatag ctctgtcgtc cctgcacaga tcttaacgcc agaaaatctc
    32221 agtgaagtac tggattcacc tccagcgttt cttgttttcg atggacagca ctacccaaaa
    32281 gatacagagg tgtggaaaca gttggcagga gttgattcga aatttttgat accagatact
    32341 agtcgctttc gcatctggct gcccgaaaac aaaattgtga tccgtgactc ttttcaaacg
    32401 agaaagcatg ttgaaagtta tgcagataaa acagatgact tttttagtga tgactatctt
    32461 tttttccacg cagacggata tattggattt tctgatttca cgatcgaagg tagccgttat
    32521 ttcgataaag ggttccctag ccgagcgctt gctatccatc taacgtatat tgatgcttac
    32581 ggcaatatac gcgtgaaaca ttttgtctct gacagtaacg actctgctaa agaccaagca
    32641 ttgaagtttc ttgaagcagg agataaaatg aaaaaatgga tcatgcgtca ccatcatcaa
    32701 ctcttgatta cttcaggaat gattgaatta ttggctcttt accagcaaca aaaattccct
    32761 ggactgggag tgttgaaaaa gtggtcactt atgcatcatt tggaacttat tagtcacctg
    32821 ctagatcatc caaaagattg gcttaaatat tattcaaaag ttcaggaact atatgaagtg
    32881 atccaatgat tagataatgt aaatgaaaaa ggaatagaaa agaggaaact ggatgtatat
    32941 tcggcaaaca aggaaagaag aagtagaaga agtggtagaa atcattaatg ccgcagcgga
    33001 tttcattgct tcgcaaggct ctcctcaatg gcaaaacggg cagaaaccaa caaaagaaac
    33061 gattttgaac gatattgaaa gaaaagaaag ctatgtatta atagtggaag atcaaattgc
    33121 tggaacagct gcattagtct caagcgtgga tcccgtttac acaaaaatag atggtagttg
    33181 gaaagaatca actggtcctt atttatcgat tcatcgtgtc gctttagcag aaagtactcg
    33241 aggaaaaaaa ctagcaaaac cgttcttaga acatttgttg acgattgcag ccagcaaaaa
    33301 tatccaagat atccgtatcg atacttatcc agctaatgaa ccgatggaaa agaccatcta
    33361 ttctgctgga ttcacttatc aagggatgat tgaatttcct tttgaacacg gcgaaagaaa
    33421 agcatatcaa ataatcatca attgactata aaatcatctg agcaaagata aagacattaa
    33481 cactactgaa aaaaacaatc atatgatagc caagaggttg taacagaagt ggccagcttc
    33541 aaaaaacaag aaagaatttc cgaaaatgct tctcaaattt ttgtggaatt ttagcgtatt
    33601 ttacgaaata gctgctttct gaaactccct tcatcgagag gtcgtgacat agcttttgtc
    33661 acgacctctc ggcttatcct acgagtatta ttcgatacat ttatatcaat aatccttttt
    33721 ttattttatc tgcagcttct tggaacgctc catcgtttga cacgactaaa agtgagggga
    33781 tagctttcaa acgtttcaac tgtgctaaat cttctacata aaccgccagt ctggctgaaa
    33841 tctgttcaga tgaatctcca cgttttttca ggcgcgattg caatgtttct ttagagatcg
    33901 taataaagat aggacggact accgtcccga aatactcata taaatgccaa aaaccatttg
    33961 ctgtcaaaac ggcgtaacaa tttcctactg tcagtttctt tttaatttct gctttactgg
    34021 aaccatattt atgttgagca tattggtccc actctgcaaa ctcatttccc ttgatcatct
    34081 gattgaatgt attgtcagaa acaaaataat aatcctcttg atctttttcc ccaggtcttg
    34141 gtgatcgatt cgtgtaagta atgatctttt gttccggctg aaaaatagct tctgcgagtg
    34201 aagtttttcc agaaccagaa ggaccgataa atacatagca ataattcatt gtcatttctc
    34261 ctttgatgat tgaacaagtg aaaaagcttt ggaaaatatt tccaaagctt ttaaaagtaa
    34321 ctttgctttt ttgtatatta taccagcaag ccagtcacaa tagcacttaa aaagctgact
    34381 aaagtcgctg ttagcaaaat tttcaatccg tgttttgcga caagatttcc tttttcttca
    34441 tttaatccct tcatcgcacc agaaatgatc ccgatagaag agaagttggc aaatgaaacg
    34501 aggaagacag atataattgc tgttgtacgc tcagtaaaat ggaaattacc atttgctaat
    34561 tctgtcatag caacaaattc atttgtaacc agtttagtcg ccatgatact accggcatcc
    34621 actgcttctt tccatgggat tccgctgatg aaggcaagag gggcaaagat gtaacctagg
    34681 atctcttgga aagtgatgcc gaatatcccg tggaagatag cattgatcat ggcaatcaaa
    34741 gcaacaaaac cgataagcat agcagcaacc gtgatcgcca catggaaacc atcaagaatg
    34801 tattctccca acatttggaa aaatgtctgt tctttgtttt cttcaatgat cagctcatct
    34861 tctttttctt ctaatacata gggatttaca attgaagcca gaatataacc gccaaataag
    34921 ttcaaaacaa gggcagtgat cacatattta gaatcgatca gtgccatata agatccgacg
    34981 atcgacatag aaactgtaga catagccgat acactcatgg tgaacaaacg tttctcagat
    35041 aggaagggta attccttttt tatggaaata aaaacttccg attgtcctaa aatagcagaa
    35101 gctactccat tataagattc caatttgccc atcccgttga ttttacttaa tcctagacca
    35161 acagctttca taaataatgg aagaatcttg atatagcgta gaataccgat aatagcagaa
    35221 ataaatacaa ttggcatcaa tacggaaaga aagaaaggtg cacttccttt atttgctaca
    35281 ccggcaaaaa caaagtcaac accttgccca gcaaaagcaa gaagatgatc aaacattttt
    35341 gccaatacag aaacaaccgc tgtaccaaaa gttgtacgta ataatacgaa accaagaaca
    35401 agctgtaaaa cgaacatcac aattagtggt ttcaatttga ttgctttttt gttactgctg
    35461 atcaaaaaag caagccctaa tgtgacaaat aaaccaataa tacctaataa ataactcaaa
    35521 taatcactcc tattccaaat tccgttttat tttacctgtt ttgcaaataa aatactaatc
    35581 tttagtgaaa ttttcattac ttttttagag tgaaacgcat tcattttcca ttagaaaata
    35641 aagcgaacaa aataaaaggt ttaaaaagtg taacgtctta gtaaagtcta taaaaaaacg
    35701 aaactatcga ttaagctaat agtttcgttt tcaacattta taagtaaact ttttctattg
    35761 gtaatataga ttttcggaag ggtagacagg tgcgcaggta atatcgattc ctagtttttt
    35821 caatgtttgt tcatcatttt tattgaccat cacagtagag tgggcttgtg ctccctcaag
    35881 ttcagctagt ttatcataag caacttgagc gattggattc gttactgcac tgattgataa
    35941 agcgatcaag acttcattga tgcttagtgc atccaaagag gaatgaagtg tctttgattt
    36001 caatccttgt attgtttcaa gaactaatgg tgacaataaa ggaatgtcat ctgcgatatg
    36061 ggctaaatgt tttactgcat ttaagataac tgccgctgac gcatccatca atgaacttgt
    36121 acgcccggta atgatgactt catctggtaa ttcgattgca atcacagcag aaggatcttt
    36181 tgtaggactt tgcgcttgaa tcttatctgc atagtcatgg gcagggacta ccacgtttcg
    36241 gtcttcagga cgtaaaccga cttcttccat aatcagtttg attcgattaa ctgcttcttc
    36301 gtcgatcaat ccttttttga agtcgcattc tgtttggaaa taacgacgga tgatttcttg
    36361 tttagaggct tcttggacaa cggtatcatc cacgatccca aatcctacac gattcacacc
    36421 catgtcagta ggagattgat aaacagattc tttaccagta attttttcaa taatacgttt
    36481 gattacagga aatgtctcaa catcccgatt atagttcaca gctactttat tgtaggcatc
    36541 aaagtggaag gaatcgatca tattgacatc ttttagatcg actgtggctg cttcataagc
    36601 aatgttcagc ggatgtttca acggaacatt ccatactggg aaagtttcga acttggagta
    36661 accagctgca tgtccttttt ggctttcatg atacagttgg ttcaaggaag tagcgagctt
    36721 tccacttcct gggccaggag ctgtcactac aacgattggt ttagttgttt caatatactc
    36781 attttttcca aaaccatctt ctgagacgat tttttctacg ttgactggat agtcttcgat
    36841 ttctgagtgt ttatagacct tgatgtttcg tctttctagt ttattgataa agactttcgt
    36901 ggcaggctgt ccactatagc gagtgataac gacactgttg attaataagc cataaccacg
    36961 taattcatcg attaaccgca agatatccat atcataagta atcccataat caccacggat
    37021 cttattacgt tcaatatctc cagcatagac gcaaatcagt atttcagctt gatctttcaa
    37081 tttttgcagc aatttgattt tcgcatcttc ttcaaatcct ggaagtacac gtttcgcatg
    37141 cttgtcgtca actagcttcc caccaaattc taaatataat ttgtcatagt ggttcactcg
    37201 ttccaaaata tacgctgact gctcttcaat atatttttct gaatcaaaac caattttttt
    37261 cactaggttc cctccttaag tactaaagca ttttttcatt tttttgagga ttcgtcaact
    37321 ggaagagcga aattcctcaa aaaaacaaag aaaatggttt aaaccaactt gtctagatag
    37381 ttattcattg gtatgatagt ttttgataag gaaggtgcta catgaaaaaa agacagaaaa
    37441 agaaaaacgc atataaacac tatatccgtt cgatttttac cggttatgaa agaatgcttg
    37501 aagatcccga attggaacaa ttgacgttta cttatctaaa cgaagaaacg cagctaactc
    37561 gtgatgagca tcagcgcatt catttcacaa caagagatct gcctagtaaa taaaaaataa
    37621 gataaaggtc atcggattcg attctgtcct atcagatgga aagactagtt gatcgaaaaa
    37681 aaggctgtac caaattttgg tgcagcctgt tttatggagc attgcccgct cttttttata
    37741 acaatttttt acagaaaatg gtactattga agtattaata agtgaaaaac agatgagtaa
    37801 gaggaggcaa gtagtatgaa agagccagat acaaatgcaa attatgagcc agacgaacta
    37861 gaggagacga atgcggatca ggatacagga caagaatcgt cttcttttta cgatatcaaa
    37921 gaaaaagcaa gcatgaatct ttcccaatta ttagaagact tacaggcttt tgaaaaagct
    37981 gtccgcgaag aagatatccc tgagatctac cgtttataca atggccgatt gaatgaagaa
    38041 ttaaaaagaa gctcaaatga aaatcacgag atcgatcaat tgctgatgcg taagatccat
    38101 gatcgttttt tacaggaatt tccttttatg gaacaagtag aaaacatctc gccaacgatg
    38161 agctattaca aaatcggcac ttattaccat gatcggccca cggtcggtat tgatgcaagt
    38221 ctgccagaaa tttttgtttt gcctaagatc gatgaagagt gggagaaata ttcccaaccg
    38281 gagtcaacgg actatgacaa aaaaatcaat gagctagacg caaaggtgat cactgctcag
    38341 acagaaattg aacgtttaga tgaacaaata aaagaaatca atcgacaaat gactgatttg
    38401 aacgacaata aaggattctt gaatcgtaaa aaaatcgagg aagaaattca agaattagag
    38461 aagaagaagc aagtactatc taatgaaaaa ttaggctggc ttccttatat tgagacgcct
    38521 gaaacaatcc agcagcaaaa agaagcattg aagcaagaag caagagcaga ccagcttcgt
    38581 gcagccatcg tagaaaaaga acaaaggcaa atcagacgct atttcggtag caaagaggga
    38641 ttcggtcaag caatccatga attcttgatg aattatcttg gcaatgagaa tcctaataat
    38701 caatctaatg aaggaggttc cgagtatgaa tgaacacgat gaagcagaat ggacaaaaaa
    38761 tgaagaacaa acagaagatc atgaagaaac gaacacttca ttggaagaac aaacaagtaa
    38821 tcctctggat gacacagaag aaatccctat gctagaggaa acaaaaatac acgaaaaaga
    38881 aaaagtaact ccgacgaata cgaatactgc agaaaacacc catgcttacg aaataactga
    38941 aaatcccggg tatttagaag gaaaagagca agatcatcaa gatatcgtca gtttgattga
    39001 aacagcggaa gcagaattag cggatttacg tatgagaaaa gatttgatcg aatcagattt
    39061 tccacaatta ttagaatcat atgatcgttt tatattgaat gaagaaccaa tctccacgat
    39121 cggaaagaca agcctgatcg aatacttcac gtatgaatta ctgaaaccgt tgaatgatat
    39181 tggtctggaa cggtcaaatt cagcttatga cacatggaaa acaagacatt tttcgatcaa
    39241 ttatacgttg aatggacaaa acgaattagt cttttccttt gtattaccga ccatcgatca
    39301 acgataccaa gtggaatcga tgagtctcct aaacgtatca ccagagacaa tggaaatcaa
    39361 tgtccaagat gaccgggtgc tttctttgat ccgacactgg tcagtggatc gagtattctc
    39421 tgcagggcag atcacgatat tcaatcacaa attgaatcaa ttattagccc atgcaagaac
    39481 acttggtttt accgttaatc agacattact agacaatacg aagccacttc gtttgaatct
    39541 tcagagcgaa tttgaattga cgaatgaagt attggatgat atttttatcg tggcaatgaa
    39601 aaatccatcc tacgatatgg aaaagatcaa cgaaactact tatcaagtac gattagataa
    39661 aggacagtct ttaacgataa caacaaacga aaaagatcaa acagtcttgt cgatctcatc
    39721 tggagactac ccacgatctg tcattgattt ctttatcaat tacgaattct tggtcccatt
    39781 aatggtgaga aagtcctgat tggataagtt ggattaccag atgtcattca gataattctg
    39841 ttccaaagga cttgcggcag ttatccagaa taattgtcac aagtcttttc tgtatgtact
    39901 aaatggacgt gaataacttg ctgatgaagg agggatttca ttaggaatgt ccacaaataa
    39961 aatagcacag cttagcttat tgtctgctgc ttgtgtagca ggtaggatcg cttttcaatt
    40021 tattccgaat ttccagccaa tgactgctat ttttttattt atagtacttt acttaagctt
    40081 aaaagatgcg ttaattgtca tgagtctttc tattgcgatc agcagttttt attttggtgt
    40141 tggaccatgg gtcatcggcc aatggatcag ctatactgtt gttctgagtt tgttttcaat
    40201 cgtctgcttg agaaaaacag tgcaaaggaa tctatggtta aagggagtct gtttttttct
    40261 ggcgggcatg gtttacggaa taggtatgac cgtttttgat acgctgttat atcgtttgcc
    40321 tcaaccttgg atctattacc ttcaaggggt gtcgtttgat ttgatgcaca gtataggcaa
    40381 tgttgtgttc tttcttgttt ttctgccagt cgtcaaacgt ttttacaatc attcaggagg
    40441 aaaaaatgaa aaaaataatt tatagtttgc ttgcaatgag tgtcagtata ctcattttga
    40501 caagttgcag tacaacctat acacaatcgg cttccaacca aaaggaggaa gtaaaaacat
    40561 cgcaaaaagc aacaatcacg ttacaagaaa atggtcaaaa cttttcggaa aaagaagtgt
    40621 tcttcaaaaa aggcgatgat ttactgagta ttttgaaacg aaattttgaa gtcaaagaag
    40681 ataacggctt catcacttcg cttgaagggc actcacagga tgagaaaaat caactctatt
    40741 ggatgtttac tgtggacggg aaaagtgcta ctactggtgc caaagagatc aaattgaatg
    40801 atggacaatc ggtcattttc aatttgtcta aactataagt ttcataaaaa actgttaaag
    40861 ggcgaatatt gccataattc agtgagttca gttgatgaaa gaaggaaaat aacatggaaa
    40921 attttaaaaa aagcggaatg ttttccgtaa ttgctgctct aggggctaat attctagttg
    40981 ctatcagcaa atttgtcggc tatgcaatca gtggcagtgc cgccatgcta aacgaaagca
    41041 tccacagtat cgttgattgc agcaatcaga ttttgctgtt attcggtgat aaacaagcca
    41101 ataaaggaca gagcgagttg catcaatttg gtgaaggacg agcaaaatac tttttcagta
    41161 caatcgtcgc aatgatgctt ttcttcggcg gtggggcact tggggtgatg gaagcttttg
    41221 aaaagttgac gcatccatcc catgaagtgg gtaatccgat cattgttata gcaatactgt
    41281 tatttggttt ggtaatcgaa ggaagttcgt tacgcgtggc aatgaaagaa atcaaagaat
    41341 tgaatactga aaaactttcc acgatgcgct ttttgcgcga aagtcgacac agtgaaatac
    41401 tgattatttt tacggaagac ttgtgtgctg taatcggttt ggtcttggct ttagcaggaa
    41461 caatattgac ttatgcaacc gggattgctg catttgatgc gatcagcgga ttgctgatcg
    41521 gtttcttgct gatgggagcc gccattttcc tagcaaaaga attttatagt ctgatcatcg
    41581 gtgaaagcgt gacagcaagc gatttatcaa aaatcaagtt agcttttgaa cgtcctgaga
    41641 tcagtcggct gatcgatata aaaacagttc atttaagtcc aacggagatt ttagttgcag
    41701 caaaagcaga tatcgttggt aacaaagaga atgaagctta ctcggtcatc aatgatattg
    41761 aaaaaaatat gcgtcagtct cttcctgaca agaagatgta tatatacatt gaaacagata
    41821 aatatgaccc aaattattct cgtaaataaa acgtttattc atgaattgaa gcaaaaatga
    41881 gaaaaatata atcttgtttt taaataaaac ggctacaatt tagcgaattt atgttacaat
    41941 gagtccgctg tttatttgtt gtaattaaag gagatatttt aatgaacaaa aagaaaagtg
    42001 cgccatggct tcaagctttg catgcagtga tgccactttg tataagctat ataccagtag
    42061 gattagcttg cggagtattg ttgcaaaaag ttggatttga tcctctctta tcaggactgt
    42121 tatcgattct ggtcttttcc ggtggcgcgc agtttttagt tgcatctatg cttacgacgc
    42181 aagcatcatt tgcaacgaca ttattgatgg ttttcttttt agaattacgc tatacattat
    42241 taggagcaag tcttgccggt tttatgaaaa aagaaaagcg gacgtttttg gctgtctttt
    42301 ctcaatcact gaatgatgaa aactatgcgg tgaactactt aaagttttca actgaccctt
    42361 cctggaataa aagaaaggct ttatatgtca attggttctc aatgactgca tgggccggta
    42421 gcaatatgat cggtaatttt ttcggctcgg tgattcgagt agatgctgac cttgtccatt
    42481 ttgctttgac ggccatgttc attttcatgt ttatcatgca gatgaaaagc gcactactga
    42541 tctttacagg tttattctca ggcgtattag gggtcgtatt catggtatta ttccaaaata
    42601 ctttcggatt gattgcagca acagtcattg cttcatttgc cggatttatg ctggagaagg
    42661 cattcaaaaa agaaaaagct aagaaactaa gagaccgcgg taaagatgtc aatgagccgc
    42721 tcttcaactt cccggaacag gaggccaatc accatgacta aactatattt gctactttta
    42781 gttgcttgtc tgttcatcgt ggcatatatc ccgcgcttgt tcccgatgct ttattttact
    42841 catcgaaaag tgccatcctg gttcagtgac tggatgaaat atgtaccggt ggcattattt
    42901 gcggcgttag cttttaaaga tgttttcatt actcatgaac atctggatat tgcttggaat
    42961 atcaaaatag ctgcaatgat tctagtagca ggcgtggctt acaaaacacg ttcgatggcg
    43021 ttgtcagttt tgacaggact tgcgtctgtc tttttgctgt ctatgcttta aactgtttta
    43081 ttttattaaa aacagagatt gcgacataag ttgttcaaac tgatctatcc gaactgcatt
    43141 tgcaacagtc aattctggat gcaagtttat tcgtagggat aaaaaagatt agtgttggct
    43201 tatgatgcaa gctctttttt attttaggaa ttagagaggg atttgtcctt ctatgatttt
    43261 ttgctataat agggaagatg tttattttat gaaagaatag ttgagggaat ttaaatgaat
    43321 aaaaaattga tgcaaggaac attttggcta acctttgcta atcttctatg taaagtttta
    43381 ggggtagtct atttgattcc ctggctaagt atgatgggga acaatcaaga tggtatgctt
    43441 gctacgacat tgtataatgt tggctatctt ccttatggat tgttcctgat gcttggtaca
    43501 gtcggatttc caaatgcgat tgcaaaaaaa gtagctgtag ccacaaaaga aggagataac
    43561 gctgcttgtc ggctgatttt tagaagtacg atcaatatca tgtttgtcat cggtatcgcc
    43621 tctgctgcac tgatgtattt atttgctcct ttactagccg gaatcagtcc gattgctaac
    43681 gttaataatg ggatactagc tatcagaagt ttatgcccat ctttgattgc tatcccaatt
    43741 cttagtgcaa tgagaggata tttccaagga aaaaatgatc tgcggcctta tggaacttct
    43801 ttgattatcg aacaggctgt gcgagtgatc gttatcttgg ctggtactta ttacttgcgc
    43861 gtattgactg acggaaccat attagaagct gtattgatca gtacagtcgc ttctttcttc
    43921 ggtgggttag ctgcgattat ccatatgtat atcgtcggac gcagaaaaga cttcttcgaa
    43981 cttaaagatt ttttgatttc cagccgttat ttcgaaagag aaaaccgatc tgcctctgtc
    44041 tctatcattc gggaaacact accgtttatt tttgtcggct cagtcattac tatcgttcaa
    44101 atgatcgacc aagtaacgat gaaaccactg cttcactttt tccgtccaga gatcgcagat
    44161 cagcaactgg aattcttatt cagccgtgct tctgtcaatc caaataaatt gacattgatc
    44221 ctgatctcca tggtaggaac agtagctata agcagcttgc caatattaag cacgatgaag
    44281 aaaaaagatc ggatacaaat cgaaaaaaca gtaggagata gtttttcgat tgcgttattg
    44341 attcttcttc cttcattgac tggaatgtct ttattagctg gaccactata cacattgttt
    44401 tttggctatg atcctgaaag tgtcggatat ttccaaatgg ctttgctagc ttctttgttt
    44461 ttctctttat tcacgatcct atctacgatg cttcaatctt tgaatcacca tcgttttgcg
    44521 atccgattaa ctgtagaagc aattatcctg aaagtcatct tccaagtaat cggattagga
    44581 ttagttggcg gatacgggat gagcttatca aatggcttag cattcggtat tgtctttgtt
    44641 cgcggctatc actttatgtg caaagaatac cgaatagcac ctttagcaaa gatcagcaat
    44701 tttttcttaa aaactttccg ctccaccctg attatgcttg cagtatgttt tgccgttttc
    44761 tttttgctga atcagcgatt gtcaatggaa tcaaaaagct atgcaatgat ttattgtgtg
    44821 gctgtaggaa gtattggcgg cttagtattt ttattttctc aattcggaaa aaatgggttt
    44881 cgaatgttga aaaatgttaa acatcatagc agaagcaaag aataggttaa ctaatcgttt
    44941 tttgctgtca cttgaaaaac ggcaactcgt atgattacgc ttgtcgtttt tctattttcc
    45001 aattttagtt tcatatctaa tcttacttat gatatagtta taaggtctgg aaaaattctt
    45061 tgaaacgagg aaattaaaca catgaaactt gcagtagtta cagatagttc ggcatatctg
    45121 ccggaacgta tcaagaacca tcccgactta ttcgttattc caatcccttt gattatggat
    45181 gggaaaattt ataacgaagg aatcgatatc gaagcggatg aatattatgg acttttaaaa
    45241 aacagtaaag attttcctac tacttcacaa cctgtcgtag gggaagtttt gggtttatat
    45301 gaggaattga aagagaaagg gtatgacacg attttaagta tccatctttc ttcagggatt
    45361 tctggctttg tcaacacatt atttgcgatg aaagatgaca tcagcggagt aacggtcatt
    45421 ccttatgact ctaagatcac tagtatgccg atgggacaca tggtagaagc tgccttagac
    45481 ttaaatgatg aaggtcagtc ccttgatgaa atacttgcac atatcgatcg aatccgagat
    45541 aatacctacg cttatctgat tgtagatgat ttgaacaatt tagtacgagg aggacgtttg
    45601 acaaatggtg cagcgctgat tggcagctta ttaaaaatca aaccgatcct gacattttct
    45661 gaagggaaaa ttgttttatt cgaaaaaatc cgttcaagta aaaaagcatt tgctcgagct
    45721 gaagaaatta ttggtagaag aaataacgaa atacaaccgc cagtcaaact ttacgtgata
    45781 catgcaaata atttagaagt agcgattgcg gagaaagaga aattgcagaa aaaatatcca
    45841 aatgcagaaa tcgaaattgg tcatttcgga ccagtcattg gtacacattt aggtgaaaaa
    45901 gccatcggca ttgccatttc agcgcaataa ttccttttat tcaaaaaaca taccaaaaat
    45961 attccaacat catcttggac tttctacatg ttactaatga gcgtgtaaaa gaacgagatg
    46021 gtgtttttat ttctatcaat gaagctcttt ttctttgcaa tcctcacttc aatcctctat
    46081 aatgtcgcat atagtttagt aaatgagtaa actactaatt tacttattta ctaaaaataa
    46141 aaggatgagg agttaaaata atggaaatac caacaaattt aaaacataaa ccagtaatcg
    46201 ttgtagaaga ctatgataaa gttgatggaa gaaatgcatt aaatacggat gcaaaagggc
    46261 tttcactggg ccttgctcag tggaatgatc gtggaaaagt cgatatctca ggcaaggtat
    46321 ggcgacatac tggagaaaaa tggtcacgac agtcggaaga attaccaatc acacgtattt
    46381 tagatttagc gatcttagct gcacaaggaa gtttatactt ccaagatgct tatcgttatg
    46441 aaaaatttta tgatccagaa aatccagtcg tagacatcat tggattacaa gggaaccgaa
    46501 tgacggtaga agtagatacc aaaaatccaa agatcgatga agacatcatt ttgtattacg
    46561 atacactaca aaaagacgga gaactgattg gggaacgatt ccgtatttta aaacgtcttt
    46621 tagatgagct aggatattaa tagacagaga tgatcaaaag gggaagagaa gcatggagaa
    46681 tttctcacta acagaaaacc gaaccttttc acggctagaa aagcaaatga tctggaagaa
    46741 accgaaaagc catcggacaa gtgcagaaga atggcggatc gcctccgaaa tcaaagggaa
    46801 ttggcaagat cctgaaatga aaatcagtaa cgtacttttg gaaggagatg ccgggtcggg
    46861 gaagacacaa ttagccaaag cactatctgc tgatctgcaa cttccctata caaaagtgac
    46921 ctgttttgca gatatggaca aatcagatgt tttcggagcc ttattacctg tagtagattc
    46981 taataaggaa caagaccaag aattactaga agctatttac caaacagata cacttgaagc
    47041 tgtactgaca ctgatccaga accattacgg cattgatcct ttttctgcta aagaaaaatt
    47101 agcagagcta gttcaaagga tcgaagctga ttatccagat gcaatccatt acagctatta
    47161 cccttctgaa atcgttcgag cgattgaaaa aggatattta ttagagattc aagagccgac
    47221 tgtcatccga gatgcctctg ttcttgtggc acttaactca gcgctcgaac caaatgggat
    47281 gttgaattta ccgacaggaa tcattcgtcg ccatccagat tgcgtcattg tgatcactac
    47341 aaatcgaaat taccaaggaa atcgcccatt aaatgaatct ttaagagatc ggatgcaaca
    47401 tgcggaaaaa atggatcttc ctgccttaga agtcatggca gaacgtgcga tggcgaaaac
    47461 tatgatccaa aaacaagaac ttctcttaaa gatggcagag attatccgtt tgttggatga
    47521 aactgcaaaa gctaacgcca tcaaaggtgt agcaggaatg cgttcttatt tttattgggc
    47581 aaatacctat aagcaaggac aagatttact ccagtcgatt tatccaaaag ttttgtataa
    47641 attatccact gattcagatg aattggcaat tcttgaacaa gctttgtctg acagtggatt
    47701 gctactggaa cttcaggaca tattgagaaa agaaaaatgg ggtcatcctc aatctgagca
    47761 tacaaaagga agaaccatta gcgccgaaga agcaaatgaa cgaaacatca ctgaaaatgc
    47821 tgaagaaatt ctgcgcttag atgaaaagaa atcgttgtct gctgaagaac caaatgaggg
    47881 agaagagaaa caagaagaaa caatagaaaa aaacgagatt cctcctcaaa tgcaaaaaga
    47941 acgcagtagc gatatcgaag gaaaaaatca aaaagaacaa caaacaggag atgattctca
    48001 agaaatgtct gcttctgaca tggaagcaca tgataaggaa atcaagaagc aactgaataa
    48061 agaagcgcga caaatcatga aggaaacgat tcatgaaaaa gaaggtctca tcgttcaccg
    48121 accagctttc caaggaaaaa cgccggaagc acgtgagcta cacatgcaag taatgccaat
    48181 agtagaatca cttagccggc aaattttaga gttactggat aatgaacaaa cagagacgta
    48241 ccaaaaagga aaatacgaag gtcagcggtt caatgcaagc aaagtagcat atggtgattt
    48301 gagaaatttt gacaaaaaga acccgccaca tgagcaacct tctttagcgg tagctgtacg
    48361 gatcgatgaa tctgggtcaa tgatacggga tgatcggatc gaagcggcaa aaaaagcagc
    48421 gattgccatt gccgaatttt ctaagaaagt cgatatccca ttgttgattt acggagatac
    48481 agcagaccgt tcgtcaagag aaaagacttc tttgttttct tataaagaat ttgaagacgg
    48541 atttcaatgg ttaaatgaaa aattagtcac tatgaaacca aggcaaaaca atcgtgatgg
    48601 tgctgcactt cgtttagcag cagaaaaatt aaacagacaa tccgcaacaa ccaaactgtt
    48661 gcttaatatt agtgacggtc agccaaaagc gttgccggat tatacaggca aaaaagcaaa
    48721 agaagatatc cagtctgttt tgaaagaata tgaacgtcaa ggaatcctat ttgtctccgc
    48781 agctattggc caagacaaag aagaaatcaa gtcgatctac ggtgaaagtc gttttgtaga
    48841 tattactgac ttgaatgaat ttcccaagca aatgattcaa ctgattgccc gctatctatg
    48901 ataaaataaa gctcaacgaa cgcacttagc agattcgttg agcttttcgt ttctagttac
    48961 cggtaacaag gatacttcta aaatagaatc ggttataatc aagagggacc gttaaaggag
    49021 tgaaacgata tgatacaaga acaaaaatgg tggaaagaag cagtaggcta tcaaatctat
    49081 cctgctagtt tcaaagatag caatggcgat ggtcaaggag acatcaatgg gatccgtgaa
    49141 aaacttaatt atttgaaaga tcttggtatc ggctttatct ggatcaatcc gatctatcaa
    49201 tctccatttg tcgataatgg ttatgacgta agtgactatc aagatataga agagagattc
    49261 ggaacgatgg aagattttga tttattgcta aaagaagcac atgaattagg gatcaaagtg
    49321 attatggatc ttgtcatcaa ccatagttcg gatcagcatc aatggtttga agaatcgaaa
    49381 aaatctaaag aaagcccgta tcgcgattat tacatttggg tcgacggggt agatggcaaa
    49441 gaaccgaata attggacatc gattttcggc ggttctgctt gggagtattc tcatgaaact
    49501 ggacagtatt atctgcatgt ttttgcaaaa gaacaaccag acttaaattg ggaaagcgag
    49561 aaattgaaag aagaactatt caatatgatc cgatggtggt tggataaagg gatagatgga
    49621 tttcgtttag atgcgatttc tcatgtaaaa aaagatgaat attcagtaaa agccacagag
    49681 aaccctttct caccctttca aaatgtctca ggaatcgaag aacatttgac cgatttgaaa
    49741 catgtttttg aagagtacga tatcatgact gtcggagaag caagtggtgt cactgcagaa
    49801 gaaggaccac aatgggtagg gaaagacggt tatttcgata tgatattcga atttgatcat
    49861 atccatatct ggcagcaaga aaaagaaggg caattagatg tccttaaatt gaaacttgca
    49921 ttaagcgctt ggcaaacttc attggatggt atcggatgga acgctttata tatggaaaac
    49981 catgatgttc ctcgtgccgt ttcagtgttt ggagatactc gtcccgattt ttgggctatg
    50041 tctgcaaaag caatcgctat gatgtatttc tttttgcaag gtacgccatt tatttatcaa
    50101 ggacaggaaa tcggaatgac gaatatgccc tttgaatcga tcgatcaagt tgatgcagta
    50161 gattcgaaaa gactttataa aaggctgctt gcggaaggta agacaagaga agaagcattg
    50221 gatatcattc gtgaaacaac gagagacaac agcaggacac ccatgcaatg gacttcggaa
    50281 caatatgctg gtttttctac acatgaacct tggctgatca ctaatccaaa taccaaaacg
    50341 atcaatgtag aacaacaaga atatgaacca gagtcggtcc tgcaattcta taagaacatg
    50401 atccgcatta gacaaaccaa taaaggtctg atatacggtt cttataaaga atatcttcat
    50461 gaacatccac aactttatgt ctatgaaaga tatttagaag atgaacaata tcttattatg
    50521 gtaaacttaa cagaatcact tgctgactat gaacttccaa aagaagcaga tcagtcctgg
    50581 acattactct tgtcaaattc ctctagcgga gaatttgaag ctaaaggcat actagcacct
    50641 tatgaagctc ggttatataa aacaaacaag taaaacttcc tcgttaaagt tcattagttg
    50701 gactttgcga ggaagttttt cagattgctc tgtcaattac tcatttcaat aattcgctct
    50761 aggaccaccg atatatttag gacgagagcg aagggctgtc acgtgagctt gtccaagggt
    50821 tttcaaacga ggtctgacag gtacttggac atgtttgata tgcataccga ttgatgtgtc
    50881 accgatatcg ataccggctt gtccaactac gtgttccact tcgacaggat cattgaactg
    50941 gtcaaaagca gcaacagaac aagcacctcc agcatgtagt gcaggaacaa cagagacaat
    51001 ttcgaaattt tttgcttctg ccactgttcg ttctactact aatgctcggt tcaaatgttc
    51061 acaaccttgg acagctagcg caatttcttt aggatccaac aactctttta acgtacggat
    51121 gatccattga ccaacttccg cactggaatt ttttccaata tgtcctccaa cgacttcgct
    51181 tgtgctgcat cctaaaacaa aaagatcacc ttttttcaag tgtgcctcag ccaatatgtc
    51241 attaactatt tcagtcaatt ggtcttttaa ttctttttct gacaacttca ttttctaaaa
    51301 ccttcttttt caattaatat tgcttccgtg cttgaatacg ttgaagactt gcagttatag
    51361 gaaaggcaag tcccattcca atcagatttt ggaagatgtt tcctggtata gaagccactc
    51421 cagccggcca atcatataaa atccaaccgg cgataccata gccgatgatc ataactaagc
    51481 ttcctaataa aagccctaat ccccacatac gagtagaaga tttgctgaaa gaaccagcaa
    51541 catatccttg taacccgtgg atcactaatg aaaaaactgc ccactgcgga tatcctgaaa
    51601 tcaaatcgat caatagacca ctggaagcac caacaactaa tccacccgtt ggaccataaa
    51661 gaaaagcagc ggtatagatc ccggcctcgc ataatgtcac aaatccattc gtagccggta
    51721 cagggatcaa aattgttaat gaaagggcaa ctgtcaacgc aatcagcata gcatagatcg
    51781 ttaattgctt agttttcatt tctcattcct cctttttgaa taccgccaga ccagacactt
    51841 ccggtatgat cgttcatcag cacgccatta gaaatacaat gggcaacaaa ttttttactt
    51901 tctgctactg ctttggtaaa atctgcccct aacacaagat tcgctgttat tgcagaagaa
    51961 aaggcacaac cggccccgtt tactgttgta gtgtcgattt ttttatgaga aaatacctca
    52021 taatgatcaa ttgaatagag aagatccaca gcttcatctc cagccatcct tgtgccgcct
    52081 ttgatcacaa tgttttttgt tcctgaagca gtgagttctt tagcgagatg gatcatttct
    52141 gttaacgaag agggaagagc ttgaccagaa agtaaagcag cttctgccaa attaggcgta
    52201 atcatcgtaa caagtggaaa aagttcttcg atgagctgcg aaatatacaa ttgattgatc
    52261 acagcttcag tttctttgaa tgccaaaaca gggtctaaaa tgattgggat attttgtttt
    52321 gtttttatga agtctcgaac aaccatgatt gcttcacttg aattcaataa cccaattttg
    52381 ataccatcta atgaagtcat tttatcaatc gaaactaact gtcgtttaag caattccaaa
    52441 gatatagatt ccagcataaa tcgctcatta atatctagtg cccctataga agtcaaggcg
    52501 cttagtccga atagaccaaa attctcaaaa gtttttaaat ctgtttggat tcctccacca
    52561 gcaaatggat cagaaccgcc aattgtcaag attcttttca taacattttc ctccatgaat
    52621 aagagtataa cgaaattttc aaaaaagaaa aacagccaat tggcatattt ttatctcatc
    52681 caatttaaaa aaacaaaaag aaaagaaaga ccaaaatgga tctttctttc cttaaatagt
    52741 taggactttt gtgattttaa ttctttctcg atttgaagcc gttgcacttt cattgtagct
    52801 gttcgcggga ttgcatcaaa atcacggatg atagggacgt tcaagcgagg aagttcatgg
    52861 acttgctccc accatcgatt ccaatccatt tctttatcag gagctaaagc aataatcggt
    52921 tgtggagaat gatttttatc ccttacaatc actacttctg ccaaaaaatc taacgaatct
    52981 aataaaaagt cttcaatagc taatgtacta ttgatcgttt cgatcaaatc cacttgccga
    53041 tctttcaaaa ataggtggcc atgcttatcc ataaaaccgt aatctccgct atcccaccaa
    53101 tcgccataaa cattttcctg gaaccgtgca tcttccttgt aataagtcaa agcccgtcct
    53161 ttagacagta attgtatatg accatctgtg ttttcaggta ggacattccc aactgaatct
    53221 gtaatccgag cgcttgtcat gtcttccagt ccaacgccca tatctcttgc atcacttgtt
    53281 ttcaaactct ctagtgtatg agccttaaga atcattggtc cgcattcact ttggccatat
    53341 acttgaagaa ataccgcatc ctgtgccatc gacgttcgca aaaatgtctt cattgtctgg
    53401 ttgtttatag catcaaaagt ggagtgatag tatcggatac ccgaaaaagc ttctggatgc
    53461 tccttagcag taaaacgcca ctgaacaaag ttattcggat gggtctctag tgcaattggc
    53521 cggtgagctt ccaacatatg aacaactttt gaactttgtg cgtttgctaa aggcatcata
    53581 ggaaatccca tggacatcaa agaagagata ccaatattga atctggaatg cactggagag
    53641 atgtgaaaag cgattaattt cttttccgaa atttttgtga aaactgtttt ttgccatttc
    53701 gtccgccatc ccatagaatg agcagaatga caaataagtt tagggatacc agttgtacca
    53761 gaagtatggg tcatataaga aatttcattt ttgtctaaaa atgattgaga aacagacaaa
    53821 gcaggttgtt ccattaaata aagaatagga a
//
LOCUS       DACDTO010000011        50609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDTO010000011 DACDTO010000000
VERSION     DACDTO010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.011"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..463
                     /locus_tag="IWK70_000687"
     CDS             <1..463
                     /locus_tag="IWK70_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP9613256.1"
                     /translation="FSIKELNYELEYEKSICSIPYNNIEEIIEKDAISIYKFFIFIPN
                     RQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESIYNLKKPLRIAIG
                     DSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFERILTI"
     gene            744..1964
                     /locus_tag="IWK70_000688"
     CDS             744..1964
                     /locus_tag="IWK70_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9613257.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            1961..4681
                     /locus_tag="IWK70_000689"
     CDS             1961..4681
                     /locus_tag="IWK70_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9613258.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            4665..5609
                     /locus_tag="IWK70_000690"
     CDS             4665..5609
                     /locus_tag="IWK70_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9613259.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(5678..6688)
                     /locus_tag="IWK70_000691"
     CDS             complement(5678..6688)
                     /locus_tag="IWK70_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9613260.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(6868..7212)
                     /locus_tag="IWK70_000692"
     CDS             complement(6868..7212)
                     /locus_tag="IWK70_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9613261.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7205..7639)
                     /locus_tag="IWK70_000693"
     CDS             complement(7205..7639)
                     /locus_tag="IWK70_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9613262.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            7777..8514
                     /locus_tag="IWK70_000694"
     CDS             7777..8514
                     /locus_tag="IWK70_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613263.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            8507..9718
                     /locus_tag="IWK70_000695"
     CDS             8507..9718
                     /locus_tag="IWK70_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613264.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            9925..10704
                     /locus_tag="IWK70_000696"
     CDS             9925..10704
                     /locus_tag="IWK70_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9613265.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            10774..11436
                     /gene="trmB"
                     /locus_tag="IWK70_000697"
     CDS             10774..11436
                     /gene="trmB"
                     /locus_tag="IWK70_000697"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9613266.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(11534..13288)
                     /gene="ade"
                     /locus_tag="IWK70_000698"
     CDS             complement(11534..13288)
                     /gene="ade"
                     /locus_tag="IWK70_000698"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9613267.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13420..14349
                     /gene="cpaF"
                     /locus_tag="IWK70_000699"
     CDS             13420..14349
                     /gene="cpaF"
                     /locus_tag="IWK70_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9613268.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14306..15355
                     /locus_tag="IWK70_000700"
     CDS             14306..15355
                     /locus_tag="IWK70_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9613269.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15352..15630
                     /locus_tag="IWK70_000701"
     CDS             15352..15630
                     /locus_tag="IWK70_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9613270.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            15605..16075
                     /locus_tag="IWK70_000702"
     CDS             15605..16075
                     /locus_tag="IWK70_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9613271.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16092..16394
                     /locus_tag="IWK70_000703"
     CDS             16092..16394
                     /locus_tag="IWK70_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613272.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16411..16782
                     /locus_tag="IWK70_000704"
     CDS             16411..16782
                     /locus_tag="IWK70_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9613273.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            16754..17113
                     /locus_tag="IWK70_000705"
     CDS             16754..17113
                     /locus_tag="IWK70_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613274.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17285..18292
                     /locus_tag="IWK70_000706"
     CDS             17285..18292
                     /locus_tag="IWK70_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9613275.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18317..19501
                     /locus_tag="IWK70_000707"
     CDS             18317..19501
                     /locus_tag="IWK70_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9613276.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(19938..21521)
                     /locus_tag="IWK70_000708"
     CDS             complement(19938..21521)
                     /locus_tag="IWK70_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9613277.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22099..22242)
                     /locus_tag="IWK70_000709"
     CDS             complement(22099..22242)
                     /locus_tag="IWK70_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9613278.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22464..22943)
                     /locus_tag="IWK70_000710"
     CDS             complement(22464..22943)
                     /locus_tag="IWK70_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9613279.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23016..23300)
                     /locus_tag="IWK70_000711"
     CDS             complement(23016..23300)
                     /locus_tag="IWK70_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613280.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23378..23572)
                     /gene="ssrS"
                     /locus_tag="IWK70_000712"
     ncRNA           complement(23378..23572)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWK70_000712"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(23644..24930)
                     /locus_tag="IWK70_000713"
     CDS             complement(23644..24930)
                     /locus_tag="IWK70_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9613281.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25068..25739
                     /locus_tag="IWK70_000714"
     CDS             25068..25739
                     /locus_tag="IWK70_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9613282.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            25705..26193
                     /locus_tag="IWK70_000715"
     CDS             25705..26193
                     /locus_tag="IWK70_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9613283.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26206..27153
                     /gene="prmA"
                     /locus_tag="IWK70_000716"
     CDS             26206..27153
                     /gene="prmA"
                     /locus_tag="IWK70_000716"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9613284.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27154..27918
                     /locus_tag="IWK70_000717"
     CDS             27154..27918
                     /locus_tag="IWK70_000717"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9613285.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28151..30364
                     /locus_tag="IWK70_000718"
     CDS             28151..30364
                     /locus_tag="IWK70_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9613286.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30375..30821
                     /locus_tag="IWK70_000719"
     CDS             30375..30821
                     /locus_tag="IWK70_000719"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9613287.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            30992..32251
                     /locus_tag="IWK70_000720"
     CDS             30992..32251
                     /locus_tag="IWK70_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9613288.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32340..33287)
                     /locus_tag="IWK70_000721"
     CDS             complement(32340..33287)
                     /locus_tag="IWK70_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9613289.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            33681..34973
                     /locus_tag="IWK70_000722"
     CDS             33681..34973
                     /locus_tag="IWK70_000722"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9613290.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            34988..36757
                     /gene="aspS"
                     /locus_tag="IWK70_000723"
     CDS             34988..36757
                     /gene="aspS"
                     /locus_tag="IWK70_000723"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9613291.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            36866..37741
                     /locus_tag="IWK70_000724"
     CDS             36866..37741
                     /locus_tag="IWK70_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9613292.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38007..39890
                     /locus_tag="IWK70_000725"
     CDS             38007..39890
                     /locus_tag="IWK70_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9613293.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(39965..40249)
                     /locus_tag="IWK70_000726"
     CDS             complement(39965..40249)
                     /locus_tag="IWK70_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9613294.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40239..40973)
                     /locus_tag="IWK70_000727"
     CDS             complement(40239..40973)
                     /locus_tag="IWK70_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9613295.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41102..41725
                     /locus_tag="IWK70_000728"
     CDS             41102..41725
                     /locus_tag="IWK70_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9613296.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            41917..43038
                     /locus_tag="IWK70_000729"
     CDS             41917..43038
                     /locus_tag="IWK70_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9613297.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43045..46173
                     /locus_tag="IWK70_000730"
     CDS             43045..46173
                     /locus_tag="IWK70_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9613298.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46192..49386
                     /locus_tag="IWK70_000731"
     CDS             46192..49386
                     /locus_tag="IWK70_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9613299.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(49532..50239)
                     /locus_tag="IWK70_000732"
     CDS             complement(49532..50239)
                     /locus_tag="IWK70_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9613300.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16532 a         8875 c        10527 g        14675 t
ORIGIN      
        1 attttctatc aaagagctaa actatgagct tgagtatgaa aaatctattt gttcaatccc
       61 ttataataat attgaggaaa ttatagaaaa ggatgctatc agcatttaca aattttttat
      121 ttttattcct aatcgtcaat tgaaagctaa tatatttaac catttgaaaa agatgctaaa
      181 tgtgtctgtt actgaatcgg cacctgtaaa tattgaaatc gtacctcaaa atgtatctaa
      241 aggtgtggtg tttgatcgtt tggaatcaat ctacaattta aagaaacctt tgagaatcgc
      301 gatcggagat agtttaaatg acttatctat gtttgaaagt gcagatatta gttttgcaat
      361 gggaaatagt catcaagaaa tcatgaaaaa agctgattat gtcactgttt caaatgataa
      421 agacggagta tctcaagcat ttgaaagaat tcttaccata taaactaatt aaggtagtat
      481 agaacataga attctaaata cggaaaaatg gagcataaat tccagtacaa atagtacttc
      541 ttcagtgtca aacgaagcga atataacata gatatttagg attgtgacaa aaatcatgtc
      601 acaatctttt tttcgaataa acttattttt tgaagtcggc catttatcgt aatttctttg
      661 cataatacga acaaattttc ggttattgat gaaaaaaaac attgaaaatg gtatgataaa
      721 agaaaaaaag gaggaaattt ttaatggtga aatttatcca tgcggcagat ttacatatgg
      781 atcggtcatt tgaaggactg acaactcttg ataaaactgt acaggaaaaa ctattgaaga
      841 cgaatctgac cgttttatca aatatcattg aacaggcaat aataaataac gttgattttg
      901 ttttacttgc aggagataat tttcatcaaa atcgtccttc attaaaaata cagaaacatt
      961 tttcagaaca gatgaaacgg ttagaaaaga accatatacc tgtatttata atattcggaa
     1021 atcatgattt ctatcaaaaa gagcggtatt ggttctcctt tcctaaaaat gtccatttat
     1081 ttacatccga aacggtagag acaaaaaaaa taacgataaa aagtggagaa actgtctctc
     1141 tcagtggatt tagttatcgt caaccgtgga ttcaaaaaga caaagtgatg gaatttcctt
     1201 ctagagaact cacagattac catattgggt tgtaccacgg ggaacctggc gtctctcaaa
     1261 aaggaaacta tgctcctttc caaccctcaa agatgcaaga aaagggatac gattattggg
     1321 ctttagggca tatccatgtg ccaacggtct taaatgaaaa gcaaacaatt gtttatccag
     1381 gtgcgcctca aggtcatact aaaaaggaac aagcttctac atctattcta ctagttgaat
     1441 taagtaaagg aagttgccaa atacatccga ttaaagtagc agaggtctat tggaagacaa
     1501 aggaaatatc attgaggaaa gcaagaacga caaaagaggt cattgttcac attcgtcagc
     1561 agctttcaaa agtagaggat ggatttagtt taattgagat aaaattaaaa gattatgacc
     1621 atctcaatac agatgttttg gaacggatac aatcaggaga gctattagac tatttgttag
     1681 aggaattctc agataggata aatgatttct ttatttggcg tatttctctc atagagaata
     1741 ctttttatac gaagacccca cttgctgctt ctgctaaatt gatggaacag ttatttcaat
     1801 actatcagac acctcaagat ttccaacaaa ttttaaatga agtatatagt catcaagaag
     1861 cggctagaat attgagcgaa ctgccggaat atcaagaaga aacacttgaa aaagccaagc
     1921 agctgcttaa tcaagatttt ttatttgagg aggatcagga atgaagattt taaggatcga
     1981 gattgctgct tttggaaaat ggcgtcaaaa aagttttgac ttttattcag gcaatcagct
     2041 gatctatggg ggaaacgaag caggaaaatc aacgatttat caatttattc aagcaatatt
     2101 atttggtttc ccttcaaaag gccgtaaaaa aaaggattat acaccaaaag acggctcagc
     2161 ctatggcggc aaaatatggc ttaaacatcc tgtatatggt gaatttgctg tcgagcgcta
     2221 caagcagcaa aatcgcggaa aatcgaaggt gtggctgggg gatcaagtgg gaagtgatga
     2281 gttactagaa aaactgatcg cacctttgac gaaagagtta ttccagcagg tatttacttt
     2341 ccagcaagag cagttgatgg aactagatca tttgagagaa aaagagctac atgatgcgct
     2401 gatttcttta ggcatcacgg gcagttctca agtattccaa aagcggcaag aatattatca
     2461 gaaagcacaa caattattca agaaaaaagg ccaaaaatta ctaatcaatc aaaaattaaa
     2521 tgaatggaaa gagctgcaag aaaaaattca tcaaaaacaa gaacaggaag cttattttgg
     2581 caagttgatc caacaagaac aggaatacga gtcgcaacag cagcagttat tggcacaatt
     2641 aaaagaggcg aacaaacaat tttcattagc cagacagcaa gaacttaatt tttctttata
     2701 tgaagaatgg caatctctca aaaaaaacag cactacaggt cttcctgatg agcaacttct
     2761 tttagatctg gagtctttct ccaaacggta tcagcaaaca aatgagcgtt tggaagaatt
     2821 agaagcaaca ctggaaaagc atagcgggat ggatcagcag tcagccagat attattttta
     2881 tttagagcag gagcaggaac taaaagatct ccagcaactt caatttacag cggaacaatt
     2941 aaacgaagaa tggcaaagat tacaaattga gactagagaa acaaattcag aattgaagat
     3001 tttggaacaa aggtggcatt ggcagaaaaa tccgttaccg gaagaatttt tatatgaaga
     3061 agaatggaag catcttctgg ctgaagaaag aaggattgct gatctagaaa gccagatttc
     3121 tgtgcgtctc caaatagcta aagaacagca agagatgatt gaagcagaag tgaatgactt
     3181 ggaagagacc tatccagcat tattacaaaa tcagtcagag atagcaccag tctcttcatc
     3241 taaaccgtat atgctgatgg ctggcggagc aataatcatt gcaggtatat ttgtaccaat
     3301 gcccttgaaa gtgatccttt tactaattgg tgcaggcatg attggctatt tcttctacaa
     3361 aaggcaacca aaaattaaaa ctaaaaatga tttctctgag ataaaaggaa tgtggcaaga
     3421 aaaactagga caacttgatc tagccttagc tcaattagct gaagcagaag aaaaacaaaa
     3481 gagaactgga gaagaaaaac agattcttca gcaagaagta tatcgttttg cggatcatcg
     3541 gcacttagga aagatggata cgctcaaact catgaaagaa tatggacagg aagtcataga
     3601 ttatcagaaa ttatatggcg tatttgagcg gaaacataaa agagaacagg aaatacaaga
     3661 aaaactacga caaatcgacc aaaaatttga gtttgttaaa gaatggttac cgatacaaga
     3721 taagacaatt tcagaaaaaa tggtattgct ttcccgtttt atacaagaga tggaacagat
     3781 gaaatttgcc agaagctatc agcaaaatac tttattgaag caagaaatca atcaattgaa
     3841 aaagaaacaa aaagtatgga tacaagaata cagcgaacaa ttgactcaag caggcttatc
     3901 ttatccttcg gaagtacctg tctttttaca aaaagccaaa caagccgaac agcagatgaa
     3961 aagatacaat gaattatcgg aaatgcttac tccactattt cctgaagagc tgacaaaaga
     4021 gaaattatct gtaagactgc gccaactgaa agaaaagcaa tcccaactcc agcaagaaga
     4081 aaaagaagca agtgaaaatc ggcaacgact ccaacttcaa gtagaagagt tgcaaagaga
     4141 cggcacattg gacgaacttt atcaacaagc tagccggcaa aaagcaaaat tacaggaatt
     4201 actggaggaa tggggagcac ttgaaatcgc cgggacattc ttaggtgatc tttcaacaga
     4261 gatgagtgaa agacaattac cccagttgtt aagagcagca ggtgattatt taggtatttt
     4321 aacaaaagga aattatcaga agatattact gaaaaatgat acactgactt taacagacgg
     4381 atatacagaa tttcccatct atgagttatc tacaggtacg aaagatcagc tgattatggc
     4441 gatgagattt gcttacctct cattagaagc taaccgttcg atatgcccga tcatcattga
     4501 tgatggctgg cttcattatg atcatcagcg aaagtatcaa ttagctgaac ttttagctaa
     4561 atttggagaa aagtatcaaa taatttgttt ttcttctgat aaagaaatgg taagctatta
     4621 ccatgagatg aaccaaatcg taaaacaatt agaaggagca aataatgaaa aaaattcgtg
     4681 atttagtaat cgatgagcag tttgaacaat ttgtcctgat caaaagtgca gatgtaagaa
     4741 tcgctaaaaa cggaaaaaaa tttattgctt ttactttcca agatacttca ggtactatcg
     4801 atggtaaata ctgggatgct tcggaggagg aaatcaataa atttacagcc ggcacagtcg
     4861 tttttctaaa cggtaaaaga gaggtttatc aaggaaaccc tcaagtaaag attcttcata
     4921 tgcgcttaac aagggtagat gagccaggag agcctagttt atacatggaa agagcccctt
     4981 tgaagaaaga agaaatggaa gaagaaatca atcaattgtt gtttgagatc accaatgcgc
     5041 attggaatcg tattgttcgc tatctactta ataaatatca aaaacaattt tttgagtttc
     5101 cggcagcaaa aagaaaccat catgcatttg ccagtggatt agcttatcat actttgacaa
     5161 tgcttcactt agggaaatca atcgccgatg aatatcctga gctgaatcga gcattgttgt
     5221 atgctggtat cattttgcat gatttaggaa aggtgattga actttccggt cctatgtcga
     5281 cagaatatac gcttgcagga aatttgatcg gtcatctggt attgattgac gaagaaataa
     5341 ctaaagcctg tcttgcgttg aaaatcagcg aagctgacga agatgttctt gtattacgtc
     5401 atatggtttt agcacaccat ggcttattag aatatggctc acctgtacgt ccaagaataa
     5461 tggaagcaga aatattacat caaatcgaca atatcgatgc ttcgatgcag atgatgttga
     5521 cttcgattcg tcaaacagaa ccaggaaaat acaccgatcg gattttcggt atggacaacc
     5581 gaagtttcta tgtaccaaaa gatttatagc aaaaatttat ttttttcatt caaaacaaaa
     5641 aaagcgagac ataagtcttc gctttttttg ttttctttta ttttgatgaa tcagttgttt
     5701 cttcagttga agattctttt gttgaatcag ttgattttgt gtcagaactc tttgttgaag
     5761 ctgtagtttc acttgagtct ttcgtacttg atgaagttgt tgtgaatgca ctcaacacat
     5821 tttcaaaggc atcatctttg atttttacat ttgcatcttt taattcttca ccgattactt
     5881 tagttgtgaa tgcattgtca cttagtttag tttcagtagc gatatctttt agttgatctt
     5941 tgtatttatc catgtcattg cctttatttt ggtttttcac cattttgaca acgtagtatt
     6001 ctgtcgcata agaagtagga tttgttgtag taataacatc ggagatctca ccgtctttca
     6061 atttgaaagc tgcttctttt acttctgcag gaatagttgt tgtcgttgag tcgaatttaa
     6121 ctttaccgcc atcttctttt gtctctgtat cagttgattt gtctttggct aatttagaga
     6181 aatcatctcc atcatccgct gattttttca catcttttgc ttcatcttca cttgataatt
     6241 tgatgatttg tgcttctact tctggatgga aagatttcca agcagttttc aaatcgtcat
     6301 ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc taaattgtta cgaatatatt
     6361 ctttataagt atctttcgta tatccagcag cttctaattg tgattcaaaa gtatcaccta
     6421 atgatttcgc ttgtttgtcg tattctgcat ccacttgctt atcggttact ttatcgccat
     6481 atttgttatt gaatacttta taaatgatca tccgttgtac taaggattgg ttcgatgatt
     6541 caagttttgc ttcatcatag aaatcagata cagtgatcgt tccgcctttc atcgttgcga
     6601 tatctttatt agtatcgcct gaacaagctg ctagagttaa aacagccaac gcactagtag
     6661 ctgccaaaat aattgatttt tttttcatta aaatttgcac tcctgtaatt agttattatt
     6721 tttatatatt aactatacca tatcccattt cctttaaaaa cgttgtcgtg gtagtttcaa
     6781 acaaaattca aaatttcatc acattcctag gaaatacgca aacttttctc acttatatag
     6841 ttaaatctgt tgagaaaata atcattatta tatttgttca ggatactctg gcagatcagc
     6901 ttttagtttt tctacttgtt cattgatttg tgtgatacgt ggttcggctt ggaatttaaa
     6961 atcttcgata tcttttttga ttgcttcttg gaaagcagga atcacagatt cagctgtttc
     7021 ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg ttatttaaat catttgtcag
     7081 agttgttgct tcttccaatt cttcgatcag tttctttctt gtttcattcc cgctcctggg
     7141 cgccaataac agacctccga cagtgccagc agcagctcca aataacagtc ctttgaaaaa
     7201 gtttttaatc atcgcaggac acctgcttta cgattgtttc ttgaatagct tttagttctt
     7261 ccgatccata actatcttga tgattgacaa agtgaataga aaaatcatcc tctttgctgt
     7321 agcgagggac taaatggata tgtgaatgaa aaacagattg ataggctagt tcacggttgt
     7381 tattcaagat attaagacct ttcatttctg gaaaagcttt ttccaatgct tgagcaatct
     7441 taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc atattcaaaa atatctgcta
     7501 catgacgttt aggcacaact aatgtgtgtc ctttcgttgc ttgagagatg tctaaaaaag
     7561 catagacctt gtcgtcttca tatattttat agcttgggat ttcttgattg atgattttgc
     7621 aaaaaataca gttttccata taaaacatcc tttctataaa gaataaatac ttcagcttaa
     7681 ctttaccata agttaaagct attgtaagga aaaaaacaaa ataccgtcat tttcttcgtg
     7741 gtataataaa taaaaataaa tggcggagga aaaagaatga gtttaattat cgatcatgtc
     7801 acaggggggt acggacatct ccctgtgtta aaagatatca gtttcgatgt acagcctgga
     7861 gagatagtag ggttgattgg actgaacgga gcagggaaaa gtacgaccat caagcacatt
     7921 atcggtctgc tggaagcttc aaaaggaacg atagaaatca atcatctatc cttaaagaaa
     7981 aatacggaag aatatcgcaa acaaattggg tatattccag agactccttc tttatatgaa
     8041 gaattgacat tgagagaaca tatcgaagtc actgcgatgg cctacgatat acctatggat
     8101 atagcaatga aaagagcaga aaagttgttg gagacttttc ggttatctaa tcgtcttgac
     8161 tggttcccag ctaatttttc aaagggaatg aaacagaaag tgatgatctt atgcgccttt
     8221 ttgatcgagc cttctttata tatcatcgac gaaccatttt taggcttgga tccactagct
     8281 atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag aaggagccgg tattttgatg
     8341 tctacgcata ttttagctac tgcagaaaaa tattgtgatc gttttgtggt cttacataac
     8401 ggagagatac gagcagacgg ttctcttgag atgttgagaa aagaatttca gctgccggat
     8461 tcttctttgg acgaaatcta tattgcttta acaaaggagc aggcacatga gtagtttttt
     8521 tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa tatatgcgtt atgtattgaa
     8581 cgatcatttt gttttagttt gtcttttctt gattggcggt gtgggattat attattctaa
     8641 ttggttgaaa accctgcagc caccgtttgc aattggaggg ctcataatct ctgtattttg
     8701 gatgctctgt ttattcgtgg gtaagatggc tacttttgca gaggcagcag atattgtttt
     8761 tttacttcct aaagaaaaag aaatgcaaac ctatttggaa cgagcttttc gctattcttg
     8821 catttttccg ttgatctttt tgatattggc atgcggattg gccatgccat tagtggtggt
     8881 tagtacggag caatcctttt cgatgttttt cgtctatctg atcatattat ggagtttgaa
     8941 attcagtcat ctgcaaatca agagagcaga attgtttcga ctagatcaac aagttattcg
     9001 ttattggaaa ttaggctggt ttttttcttc tctgatcttt ttgtttctat cattttattg
     9061 gctaatatgg gcaggaatag caggtgcatt agtacaggca agtctatatt actggttttt
     9121 gtggaagcaa caagaaaaag gattggattg ggagaaaatg gtgcatgtag aagaacatag
     9181 gttgcatcag atctatcaat ttatcaattt attcacagat gttcctgaaa ttacggcaaa
     9241 gatcaaaaga agacgctatt tggatcctat attgactcat attaaaagag aatcaaaaaa
     9301 cacttatctt tacttgtttg caagaagatt ggcaagaggt tctgatttta gtggtcttta
     9361 tttccgatta gtaattatag gtgggttatt gattgccgga gtagtcgact tttattttag
     9421 tctgattatc ggtttcttat ttttatattt gatcggtttc caactgttac ctttagcgaa
     9481 ccagttccgc tatatggttc tcgttcagct ttatccaatc cataagaatc agaagaagca
     9541 ggcgattcag tatctactca catgggtatt aattatcgcg tcggttattt ttggtttgat
     9601 tgctgcagtc gtattagatg ggcagcagcg atgggttccg ctagcggctt atttgattgt
     9661 tactatgctc tttgttaaac tatatgtacc aactcgtttg aagaagatgt cagactgaaa
     9721 acaaagctgt gtagggtaaa aatgagaatg aaaaatagag cgttgacagt aagaatgctg
     9781 ttcgttctat tttttttgag aaaagctggt tttttgaatg gcaattaaat gaaaaagaac
     9841 gtatatgtgc ttgacgtgta gtgttcaggc gactagaata ataagtactg caaaacaaag
     9901 agtaagagga cgggagaatg catgatggag tttcagttag accaagagtg gcggttacgt
     9961 cccatcaaag gcgacacagg aaaaacatac ataggcatgc gtgatgagga caaagtcttt
    10021 attaaacgaa acacgactcc gatgttggct gctctttcaa gggaaggaat tacaccaaaa
    10081 cttgtttgga caaaacgaac ggggaatggc gatactttga cagcacaaga gtggctggaa
    10141 ggtcgcttgc tgactcctaa tgagattggt caaagaaacg acgtgattga tgttttgtac
    10201 catttacatc attcaaattc tcttaaaacc atgctgaccc gtatcggtgg tcgtgtcatg
    10261 acgccggaga agatgctgca aaaatatgaa aaagaattac cggaagaact ccagaaaaat
    10321 caatttctgc aaattgtcca tcgttttctt cgtgagcaga tcccgtcaat agataaggaa
    10381 aacttatcag tagtacacgg agatgtaaac catcgtaatt ggttagtatg taaaaattat
    10441 ttatatctag tcgattggga ttcaatcatg tttgcagacc ctgcggtgga tattgcaacg
    10501 attttaggaa attatgtgcc actgtctagc tggaatcagt ggctgatcag ttatggcata
    10561 cgcccgactg acgaaatgct tgaaaaattt cattggtatg cggccatgaa tttgctccaa
    10621 gaaatcacca gatattgcaa acgtgaagat catcgacgga tgaacgaaga aattttacaa
    10681 ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc tgcggagatt tggccagacc
    10741 tttttaatta gaggaaagga atggagataa ataatgcgtg tccgaaatcg tccgggagca
    10801 gcggaaatgt tagcggctca cccgaatttt gtaattagtg atccaacatt gtggaaggga
    10861 aaatggaatg aattgtttga aaacgatcat ccaatccaca ttgagattgg tatggggaaa
    10921 ggccagttca tcactggaat ggcaaaagcc catcctgaaa tcaattatat tggagtagaa
    10981 atgcaggtca gtgttgtttc gattgcacta gacaagttga tcgaacaacc gcttcctaac
    11041 ctgaaacttc tacatgtgga tggatcagcg ttgacagagt atttcgctga cagcgaagta
    11101 gatcaaatct atctgaattt ttccgatcca tggccaaaaa aacgtcatga aaaacggcga
    11161 ttgacatata agacttttct tgctgtagat gaacagattc ttcgtccaaa tggagaaatc
    11221 catttcaaaa cagataatca aggattgttt gagtattctc tggcaagttt ttctcaatat
    11281 ggcatgatcc tcaaacaggt atggttagat ttacatcaaa gtcaatttga aggcaatatt
    11341 atgacggagt atgaagaaaa attttcttca aaaggtcaac gtatttatcg agtagaagct
    11401 caatttcaag ataagaccaa aaagaaaagt ctgtgaaact aactttctgc tttcataaaa
    11461 acattaatat ccataaataa atctcagaaa atgaacatta cagatgtaat gtattttctg
    11521 ggatttattt gatttaaaat gtatttattt catcggtttt tccacaaggt ggaatcaatt
    11581 ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt caattcgggt atgacagata
    11641 atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt agctgctcct agttgatgtg
    11701 ctttagttat taattcattc aactgttcat agacgatcga aaaaggttcc tgtgtcatga
    11761 ttccgcctat aggaagagaa acacaagcta tttcctgctg tttgttgaca acggtcatcc
    11821 cgccaccttt ttgaatcaaa agattagcag catacagcat atcctcgtcg tttgtcccaa
    11881 cgacaactag attatgcgag tcatgggcaa tcgttgtcgc tatcgctcct tgtttcagct
    11941 gaaatccttt cactatgcct aatccaatgt ttcccgtttg atggtgacgc tcgatcaccg
    12001 caatctttaa ttggtctttt tcgattgaag gaatgaatcg ttcgccttgt cggtccactt
    12061 tttctaccaa atctttagtc actagactat tcgggataat ttcgatcaca tgggcttgtg
    12121 aagaagaaag aagcagctct aacgaatgtg gatttaatgg ttgagcgttt agttttggca
    12181 gacaacacgt atatgaattt gaatgatcag caaataaatg gtcttggagt tgcccagatt
    12241 gaacaactaa tgttccctta tagaaaactt catgaattgg cagcttggta agatcgtctg
    12301 ttaagaagaa atcagcttga tacccagggg caatcgcacc tagtgttttc aaatcaaaac
    12361 attctgctgc attcaatgtt gccatctgaa tggcttgtaa tggatcgatt ccaagtgcaa
    12421 tagcttgacg gacgatatga tcgattgatc cttcttttac taaatcattg atcagtttat
    12481 catcggtgac aaagaagcat cttcttgaat tttcaggtgt gatcgccggt agtagcgatg
    12541 ccagatcttt tgcaacggtc ccttctcgaa ccattaaata cataccaaga tccaaacgtt
    12601 ctttagcttc ttttgcagtt gtagcttcat gatccgtccg aatacctgct gcaggataaa
    12661 catttaactc ttccatgccg ataccagctg catgcccgtc aatttttttc ttatgctggt
    12721 gcatcaattg gatcttttct aggatgtctg tttcattatt tgctactgct tgatagttca
    12781 tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa aggggctaat ttttttgcat
    12841 ttaaatttgc accattggtc tcaaaagggg ttacaggtac acaagaaggt agcataaaaa
    12901 agatattcat gagtgtttgt cttgcatctt ctatcataaa ctcaagacct gtactcccag
    12961 caatgtttcc tatttcatgg ggatctgtca ctacagtagt aactccatga agcaaggata
    13021 cttttgaaaa ttcgcttggt gataaaagtg agctttcgat atgcacatgg ccatcgatga
    13081 atcctggcac tagatattgc ccattagcat tatgtacgct ttttccttcg tattcaccga
    13141 ttcctacgat cattccttcg cagattgcta catctttttc catgatttct ttcgtaaaga
    13201 cgttgacgat cttagcatgc ttgatcacta ggtcagcagg ctttttttta cttgctactt
    13261 cgatatattt ccttaatgtt gtttccaatg gagtgcctcc caagataaat ttttataagt
    13321 ataacataaa ttcggctttt gccaatattt ttttgtaaat tcattttatt tgaaacaaaa
    13381 agatcaaaaa gacgtatgtt ttttgtggag gggagaataa tggaaataaa acgattatcg
    13441 gatcaaatga taagtgaaag ttttgccaaa catgcccaag acgtatatat attgccagct
    13501 tcagaagtat tttacatcta ttttcgctat ggaaaaaaac gaacattctg gaagaaagtc
    13561 tcttcggcag atggacagca actgatctca agatttaagt atttgggaca gatggatatc
    13621 ggagaaaaaa gaaaagctca gttaggtgca tttacttatt ctttagaaaa acaggaagtt
    13681 cgtctgcggt tgtcttctgt aggagattac cttcaacgag agagtttagt gattcggcta
    13741 ttatacggta cttttgaaac accccatcgt tgctttagtt ctcgagattt tgcaactatt
    13801 ttacagaaaa ccgtcagacg tggattatac cttttcagcg ggacagtcgg atcaggcaag
    13861 acttctctaa tgtatcggtt ggctttggaa gaaaaattgc aagtgattac tatcgaagat
    13921 cctgtagaga tagaagaacc gttttttctg cagctacaga tcaatccgaa aatagcacag
    13981 acttatgata cacttttgaa actcgcatta cggcatcgtc cagacttgct aatcattggc
    14041 gagataagag atgaatcaac tgcacatgct gcggtaagag cagcactaac agggcaccgg
    14101 gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat tggtcagaat ccatgaactg
    14161 acaaagaaaa aagaagagct atctgaatgt ttagctggtg tcatttatca agaacttttg
    14221 gaagatgcca atcaccatcc ttctgtttta tggagttatc aatttatgga ggaagaatca
    14281 gtaaaaaaat catgggagga aagttatgaa gaagctcagg aaaatatttg gcagtcagaa
    14341 ctattctaaa ggattaacaa gaagccagca aaaccagttt gcacaaacca tgggagaact
    14401 gcttttatct ggttttagtc tgcaagaatc cctttattta ttactcacga tccgttcgtt
    14461 tccacctgtt attttggcaa atatccaaca tcagctaaag gaagggcaag cgtttagtga
    14521 ggtactttcg caaataggat atggagcaga gcaactggta cagatcgagt tagctgaacg
    14581 tcatgggaat ctgccgacaa ctttgttgga aattgccaaa cagtttcggc ttgttgaagg
    14641 atttcgcaat gatttgaaaa agctgcttgc atatccgatg ctattgctca cctttttgct
    14701 aggtatcttg tttagtatgc ggttgttcgt tttgcctcag cttattggtt caggaatggt
    14761 catgcaagag cattggggta ttcagctgat acaagtcttt cattggtatg ttctactatt
    14821 catcctagcg gttactctat tggctttact agtaaggtat cgtctgatga agttgtctcc
    14881 tgttgatcag agtgaatgga taagcagaca attattgatt ggaccgatat attccttgta
    14941 tcaatcggct tatttgtcac tagagattgg aaagcttttt catgaaggtt tcgaattaag
    15001 gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt ctgattcagc atcttgcctc
    15061 taagttgatt caaggattgg aggcaggtat gtcattggct gagcagttcc agaattatcg
    15121 ttttctatca gaagaattca gtaaaattgt tttacaggga gaggcaaagg gaagtttagg
    15181 taaagaactg attttttaca gcgaatggac aagaaaacag cttttcaaac gtcttcatca
    15241 ctggatgcat tttattcagc caatcatttt tttgggcgta gcggctttga ttttattagt
    15301 ttatgccgct gttttattac ctgtttacgg aagcatagag gaggttttgc catgaaaaag
    15361 agaaaaatat cttattatag cggttttaca ttgatcgaaa tgctaatcgt cttactgatc
    15421 atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc gttataggaa tcatgtagac
    15481 caagaaagtc gtgaagcgat catccagttg gtagacacac aaaaagagct ctatgcgctt
    15541 caaaataatg gaagagttcc tactgtggag gaattgttga atgaaggcta tatcaaacga
    15601 gaacatgcag aaatctatca gcggccttga aggtaaggtt ttagcatcag gatatacgtt
    15661 gtttgaatca ctcttactga ttttattact tacaacgatc ctttcttttc cagttctcgc
    15721 attttctgca tggaagaatg aattggcagt ctatcaattt ttcggccaat ttgaaaaacg
    15781 aatctattct acgcaaaaaa tcgctattgt gaatcagatc caaacaggct tttattggaa
    15841 tagtgaagag aatcaaatca tttttcatgt tccgaatcca gataaaataa attgggaagt
    15901 attagatatt cctgatgaga ttaccttgaa acgccatgct tctattactt ttgctgctag
    15961 aaccgggaat gaaagttcac taaaagctta tcaattttat tgggaagaaa aagagcggac
    16021 gattacgtat caattccaga tagggagtgg tcgctataca aaaagaattg aataaaacag
    16081 gatttgtttt gttggagtcc cttacagcgc ttatgatcgc ttctgtctgt gtctacttag
    16141 tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa aagtggggag gaactgctat
    16201 ttattcgaat gatttatgaa gatatcaaga cgcagcggat gtatcacaaa agaccagagc
    16261 gaagggaaga aatgatccca tacgatatcc aaattgtcga taataatgga gtaatcgaaa
    16321 aagtgtttgt caatatggag aaagaagcga tcagtattga aaaaacaagt aattccagca
    16381 tttacgctga ttgaatgtct aattggactg atgatattga gcagtctatt gctcacattc
    16441 aatcttttga ttcagcaaac catccaagta agtgaatttt tgcagcgtaa agatcaaaaa
    16501 gagtggctaa tatttttagc acaattagaa gaagaactca agagcagtca caatgtctct
    16561 gtgaaaaatc agcaactgca ctacacgatc gaggataaac agtatatcat cgaaagatat
    16621 caggcaatga tacgtaaaag aagaacttcc ggcggacatc aacctatgtt aacaagtatc
    16681 actgagcttc aattattgga aaaagaacac acaatttctt tttatgtaca ctttgaaaat
    16741 ggagaagatg gatatgccat atggacaaaa aacgatcagt agaaaatgtt ataagggctc
    16801 tattttattt tctgtcttgc ttttatttct tttatgtacg tttttattga tggcagcatt
    16861 agaaagctac cgtttgtcaa tggatcttaa tatacgaatc aaaaattatt atatagcaaa
    16921 aataatgaag cagatggctt tatcggaaca gtctacgctt gccgaaagtt cggaaggagt
    16981 tttttactac acaactggat ctgtaacata tcagtgggta caacagtccc tattcttaga
    17041 ggtggaagtc tcaccgttta tctttcggtt cacagaagaa gtaaaaaatg atacagatac
    17101 tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt tctgttataa cactgttttt
    17161 aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg aaaacgattg aaattttgtc
    17221 aattgttcga tagaattaaa caggtgaaaa aattgttata atatatctag aaatgaggtg
    17281 caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa aacctggaag ctattcagct
    17341 tttacagaat gcattgggaa cttccttttt agaagcctat gtcgaaaatg cagaaaattt
    17401 aatagatgat tatcaagtac gtgtggtgga tggagtgccg acaaaagaga caacgcaacg
    17461 gatcacagct ctttatgaag agttgaagaa actttctttc gagccagaag aatggcgcag
    17521 actgtctcaa ttacttttat tgaagggcag ccagacagaa catttgcagg cgaaccatca
    17581 actcactcct gatagtatcg gttttttatt tgtttttctt attgaacaac tatacacaaa
    17641 taaaaaagca ccggttaaga ttctggatat tgcggcagga atgggcaact tgttattaac
    17701 agttctttta aacctatcga atgcggggta ccaaacagaa ggaattggtg tggatattga
    17761 tgatacattg ttggctgtcg cagcttctac aagtgatttg acacaagcaa atgttcaata
    17821 ctttcatcaa gacgggttgc aggaattatt aatagatcct gtcgattttg caatcagcga
    17881 tttgccaatc ggttattatc cgaatgacga aaaagcaaaa gagtttttaa caagtaccga
    17941 agaaggccat agttatgcac atcatttatt gctagagcaa tcaatgaaat atgtgaagcc
    18001 ggatggtttt ggtttgtttt tgatgccgag tggattttta gaaacggatc aaagtgaaga
    18061 aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga atgattcaat tgccagatga
    18121 attgttccgt aataaacaat cgcaaaaaag tattttgatc ttacagaaaa aaggaccaca
    18181 agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct tcattaaaag aaccagagaa
    18241 ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca tctgctctgt aatcattcaa
    18301 cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat gctgggagtt caagtttgaa
    18361 atggcaatta tatcaaatgc cagaagaaac cgtagttgcg aaagggatcg tcgaacgtat
    18421 cggtttacaa gattctattt ttaccatcaa gtacggcgat aatcaaaaat tcgaacaaat
    18481 catggatatc gaaaaccacg aagtagcagt aaaaatgctg ttagatcagt tgattgaact
    18541 gcaaatttta agctcttatg atgaaatcac cggagtaggg caccgtgttg ttcacggagg
    18601 agaaacttat ggggattcag tagtgatcga tgagaaagta atggaccgca ttgctgaatt
    18661 agcagaattt gctcctttgc ataatcctgc aaacttaatg gggatcaaag cgtttaaaaa
    18721 gattttacct gacattttga gtgtggctgt attcgataca tctttccact caacaatgcc
    18781 aaagcaaaat tatttatata gcttaccggt gtcttattat gaagatttca aagtaagaaa
    18841 atatggcttc catggcacaa gtcatcgtta tgtgtcggaa cgtgcggcaa aattattgaa
    18901 caaaccgatc gaagaactaa aaatcatcac ttgccactta ggaaatggtg tatcgatcac
    18961 agcagtcgat ggaggtaaat ccatggatac ttctatggga ttcacaccat tagctggggt
    19021 gacaatgggt acacgttcag gtgatatcga tccagctgtt ctcccatatt taatggaaaa
    19081 attagagatt gatattgatg aaatgatcaa tgttttgaac aagaaatctg gtctgctagg
    19141 cctaacagga ttgtctagtg atatgcgtga tcttgaaaag aactatgaaa aagaacatat
    19201 tcgtttagct tatgacattt tcgtggaccg catccgaaaa tacatcggcg gttatgtgac
    19261 aacgatgaat ggtgttgatg caattgtctt tactgctggt atcggcgaga atgacggaca
    19321 tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt tgtgaaatcg atccagcgtt
    19381 gaatgaattg cgtggtgaag aagtagatat ctctacaaaa gaatcaaaag taagagtatt
    19441 agtgatccca acagatgagg aactaatgat tgcccgtgat gtggagcgtc tgcgcggcta
    19501 agccaaagca ttcgttagga taaaagatga aacgcatgca agagggaatt cttgcatgtg
    19561 ttttttttgc ggagaagacc gcaaaatggt aatttaactt caatttccct tcttttttac
    19621 aggatcttct tagggaattt tgcttggaaa agtggtcaaa agggaaaaga ctggcatttt
    19681 atttattgat tttcaggaaa atcccaatat tttattttca ttcatcttta taagggacat
    19741 tacatatgta aaaagaaaaa acatttttag atcagcctcg tgggctgtcg actttattct
    19801 tcgctttatg tttagctaga aaaccgatcc gaaacattca ttgatcgaat aataaaaact
    19861 tgattttcca cctgtacttt gcctgaatcg tgaaagcaca ggtatgaaaa tcaagttttt
    19921 tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg gctggcggct gatctgctcc
    19981 tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa agtagcggtc atggctgact
    20041 gctaggagcg ttccttgata gtcttgcagc atttcttcaa tttcttctcg agcatagctg
    20101 tcgaggtgat tggttggttc atccaaaatc aggacgttga ttttttctaa aaagagtttc
    20161 agcaacgcca aacggatttt ttcaccaccg gataaatctt tgatccgttt acccacatcg
    20221 ctttgataaa agccactttt ggctaattgc cgtcgagcga cttcttcgtt tccagtcaat
    20281 gtgatggcgt aagccagcaa acggctatcg ggatcggcaa actgcagctg ctgcggcaaa
    20341 taaccgattt gcaagctact acctaatttg atggtgccac cgtaaggctt ttcctcaccg
    20401 agtaataatt tgagtaaggt tgattttcct gcaccattgt cgccgatgat ggctaaatga
    20461 tctccacgat agactgcaaa actgctgtct ttgaatagta cttgatcagc gtactgctgg
    20521 tgcaagtttt gaatgatgaa aacttctttg ccagattttc caccattaga caagctttgc
    20581 agccgatttt tcgggggagg tggcggtttg acgagtgtca gtttcgccag gcggcgttcc
    20641 agttctttgg cttttttgaa gaaggactca ttgtctcctt catgtgccca ctgacgaaaa
    20701 cgacgaatca tgactttcaa ccgctggact tctttttgct gcagttcgta attttttgtc
    20761 agctcagcca agcgagcttc tttcaagact cgaaaacggc tgtagtttcc gggatactca
    20821 atcaatgcac cttcttccag ttcccatgtt tttgtggtga cttgatcgag aaattgccga
    20881 tcatgagaaa cgaccagaac cgattttttt gtattctgta aatagttttc caaccagcga
    20941 atactgttaa gatctaaatg attggtcggt tcatcgagta aaagtaaatc ggcatcggct
    21001 gctaaaattt tggctaaagc gactttaact cgttcgcctc cgctaagttc tgttaaccgt
    21061 gcttcctgac agtgtggaat ccctaagcct tgcaagatcg ctgcaatccg gtcttctaat
    21121 acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac cgtaacgggc cagacacttc
    21181 tctaagtttg tatctggttc tgtcatcaat gcttcgtagc gactcatttg ctgtttgagg
    21241 tcctgtatct cagcggcaga atggagcaag tagtggttga cggtcgcttc ggaaactgta
    21301 agttcttgtt cgaccgtccc gatctttaag ccttttttgc gactgataac accgctgtcg
    21361 acaccttctc tgccggtcaa gatttgtaaa aaggttgatt tgccagttcc gttgatatcg
    21421 actaaggcaa ttttttcacc agcttcaatt tgacaggata aatcctcaaa taatggattc
    21481 ccgccgattg tttttttaat agaaattgct tgtatgatca tgatctctct ccttgagacc
    21541 actcaaaaag gccatggtta tccctggcct gtgttattta aactcagatt atcatgagaa
    21601 aaataccttg agatggccat tgattttacg tgaattcaac tgttttggac aaacttgtcc
    21661 cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc gatatggtat cgttggtaat
    21721 tttcattact tgttatttta gtcatgcgca tcatagtatt ttccctcctt cgtgtagttg
    21781 tcttttccag tgtacctgaa agtgtcagaa agttcaacta gcgctgacat ttacttaata
    21841 tttatcgatg tgtaattctg tcagagagct ttttttatct gactgagggt aatgtctcgt
    21901 ggaccacatc attgtattct gagctaaacc agcgttcagg cgaaatcata tggtgtttga
    21961 taaaaaactt tgtctacatg acttcctttt gccatttgct taataccgtt gcattaaaca
    22021 gctcttctct ttggaaatct caccaacatc gcgttcagga gtatcgttgt ggttgcaatg
    22081 gcaagcggta gcctttcatc aattcttgaa tgatttaccg aatgtagcac ttgccttctc
    22141 gcggtacccg aacccgaaat aaaattttag tttggcgata aaatcggtat ccgccatcga
    22201 tacttcatat tttttcgtat cagattcatc ggaaacttcc acattgacga accaataagc
    22261 tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac taatcgccct ggtgttcact
    22321 ttttttattt tacttgaact cattgactgt tttcccttta ttttaaacat tataaagacc
    22381 gatgagtgct tgccgcgtct tttgttttta tcacggtttt ttgtgcaaaa aaacctgccg
    22441 atagttggca ggtcgtctac gtgttaagct ttggtgtggt ctttagctgg caattggttt
    22501 tctaaatcag ttcggacaac aagcacatcg caagctgcgt ttctaataac atattcagaa
    22561 acagaaccaa taaataaacg ttctacagca ttcaatcctg tggctcctaa cataatcaaa
    22621 tctacttgat tatcttctgg gatttctctg gcaataattt gttttggtga gccatattct
    22681 acgacagagg ttacattatt caagccagct ttcttcgcat tgctttcata atctgccaat
    22741 gtttgctttg ccatttcagt agcttgttct gcaagcattc catcaaagga tgaaacggtt
    22801 tggaaagaac gtgtgtcaat gacatgtgct aatagaagtt ctccgttatt tcggatagct
    22861 acattgaccg cctttttaaa agctaattca gcttcatcag aaccatctac ggcaaccatg
    22921 atttttttgt attgttgtaa cataagcatt cactcctcaa gtcaatcgta ttgtactctt
    22981 ttattgtaga acactttagg gaaaaagaga attatttact gctttggttc agcaattttt
    23041 tttgcaaatc ggatactgaa ggtggcagaa acaataatta cgatcagtaa aaagaccaag
    23101 gcaataaagc gttgattgaa aaaagctgtt aaaataccca ttacgcctaa aacagcatat
    23161 gcagcaccgt aaaattgtag gaatccttga tttttatcat ttttgttgat caatacaaaa
    23221 aagatatcct gtttattaaa taaataatag ctaacgaata ataatactaa agtggcgata
    23281 atcattaaaa tcctaatcat gaacaaatcc tccaaaataa gcaacaaata gaaaaagcaa
    23341 tttgccaaga gcaaattgct ttattcttta aaagatggta atctccgtta atgccgttgt
    23401 ttgtcctgta gtattgatcc cgcgaattca agcaggtggg tcccatggtc gtagcttcga
    23461 actaccaaat gataaggcat gttaccagca gtgacacaag acgggtccca agatatcaat
    23521 aaaaatgttc ggtcaacaca caaagaggac aattcgaaca tcacagattt cccacctcta
    23581 tagtagcata tgaatttaaa aagagcaatg agagagctcg cagaaacaga accgttttct
    23641 tgtctagttt tttggaggat gctttttcca ctcttttata cggtaatact gttgacccag
    23701 agcctgttca tatttccctg tatcttttgg ctgataatac tgagcattct tcagtttatc
    23761 tggtaaatat tgctgattga cccaagcctg atcaaaatga tgaggatact gataacccac
    23821 gccacggttg agtgatttcg cacctttgta atggctatct ctcaaatgat caggaacatc
    23881 tccagctttg ccctctcgaa tatcagcaag tgctgcatct aatgccatgt aggcagaatt
    23941 ggattttggc gacaagcaaa gatcgacgac gacgtctgct agtgggatac gtgcctcagg
    24001 caatcccaat ttttcagcag ctaatacagc gttcactgta cgagccgcag cagcaggatt
    24061 acctaaacct atatcttcat aaccgataac catcagacgc cggcaaatac ttgccagatc
    24121 acctgcctca actaaacggg cgagataatg aagtgcagca tccacatcac ttccacgaat
    24181 cgatttttga aaagccgaaa taacgtcata atgagcatct ccatttttgt catgggtaag
    24241 agcttttctt tggacacatt cttcaatgat cgacagtgta agatgaatcc ttccttcttt
    24301 atctgaagga gtcgatagtg tagccagttc cagcccattc aatgcactcc gcaaatcgcc
    24361 gttcgttgca cgcgaaaggt gaagaagagc atcttcgtct aactggattg cttgttgtcc
    24421 aaggccgcgt tctttatccc ttaaagcatg ttcgactgcc aactggatgt cttgttctgt
    24481 taatggtttg acttcaaaga tctgtgtacg gctgcggatc gctggattga tggtaatata
    24541 aggattctca gtagtcgctc cgatcaagat gattcggcca ctttctaaat gaggaagcag
    24601 aaaatcctgt tttgttttat ctaatcggtg tacttcatcc aataagagga tcactgtacc
    24661 actcattttt gcttcttccg ctacaacttg tagatccttt tttgaatctg tagcggcgtt
    24721 tagcatgcga aatgcataat tagtagagcc ggcgatcgca cttgcgatac tcgtttttcc
    24781 agtaccagga ggtccatata gaatcatgga agagagcatg cgagcatcga ccatgcggcg
    24841 tatgattttt ccttccccga ctagatgctg ctgaccaacg acttcatcga tcgttcgagg
    24901 tctcatgcga taagccaatg gctgttgcat aggattttcc tccttcttgt ttttacgtcc
    24961 cttaattata tcatatttag caagatgcct agaaagaaca gccttctgtt gttttgtttt
    25021 catagcttat ccaactaata ttgtgtatga taaagtaggt gagaaaaatg acggataaac
    25081 aaaagataga tgaatttttt tggaataaaa cgacgccaga agtggcacgg gatttattgg
    25141 ggatgtattt ggagcatgac acaccagaag gaagattagc tggatatatt gtagacgcgg
    25201 aagcatacct aggtccagaa gacgaggccg cccatagcta tggtttaaga agaacaccgc
    25261 gtgtgcgggc aatgtatgaa aaaccaggta cgatctacct ttacacgatg catactcatc
    25321 ggattttgaa catcattact cagccagaag ggattcctca aggagtaatg atacgtgcaa
    25381 tcgaacccgc cgcaatgatt gatcaaatga gtaaaaatcg tggcggaaaa actggtccag
    25441 atatcagcaa cggtccggga aaattagtgg aagcattagc aattcctcaa gaactttatg
    25501 gtcagtctat agcagatagt tcgttacgct tagtttttga gaaaaaaaag acaccaaaaa
    25561 agatcaatgc tttgcctaga atcggtatcc caaacaaagg tgtatggaca gaaaagccgt
    25621 tgcgttttgt tgtgtcggga aatccttata tctctttaca aagaaaaaac caaatcgaga
    25681 aagattgggg ttggaggaaa gaaaatgaaa aagaaggatc tattaacata tttagatgaa
    25741 ataattgaaa aacaagtgac agactatgat gtagctttag attgggatac aaaaaatcat
    25801 acgatcgaga tcgttgttcg cttgtttgca gaaaataaag cacaatttga gattgatgat
    25861 gcagaaggga tcgtttcgca agaaccaatt atcgaatttg aagacggcgt acttcttttt
    25921 aatcctcaga aatctgtttt tgatgaacgc aattatctag cagtaatccc ttatgaagga
    25981 aaaaaagggt tggcaaaatc agtagctgat tctttagtgg aatatctaaa tgaagtatta
    26041 gctcaagggc aaagcgattt gcttgatttt ttggatgaag acgatgacga agctgtattc
    26101 gagcttcact gggataatca acaactagca gaattagtag aagtaaaaca gcaaaatgaa
    26161 gcagatactt atttaccgta cccaagttat taaggagtga cagatatgaa atggaatgaa
    26221 gtaaaagtag aaactgaaag tgaagcagtc gaagcagttg ctaatatttt gatggaggca
    26281 ggggcgagtg gtgtcgcgat tgaagattct ttagatgttg aacattttca gtcggatcaa
    26341 tatggtgaga ttttagataa aaatgatttt acatcgctga aagaaggcgc actagtcatg
    26401 gcttattttc cggaaacgac tttcttaccg gaaatcttgc cttttatcaa agatcgtatc
    26461 acacaattac ctgagtttgg tctagctatt ggaaaaaata aagtaacagt tagtgaagta
    26521 gaggaaagca actgggcaac agcctggaag aaatactatc atccggtgcg tattacgcga
    26581 atgctgacgg ttgcgccaag ctgggaaaac taccagacag aagatcctct agaaaaaatt
    26641 atcacactag atcctggtat ggcatttggc acaggcactc atcccacgac ttccttgaca
    26701 ttgacagcac tagaaacagt cttgcgtggt ggtgaaacat tgctagatgt gggaactggg
    26761 tcgggcgtgc taagtattgc agcaaaatat cttggtgcaa aagatgtcta tgcctttgat
    26821 ttagacgaag tagccgttcg ttcggcgaaa gaaaatatgg atatgaatga aatagccaaa
    26881 gatgtccatg tatcagctaa cgatcttttg acaggaatcg agattgaaag tgatgtaatc
    26941 gttgcgaaca ttttagcaga tatcatctta ttgatgatcc cagatgcttg gcgtttactg
    27001 aagcaaacag gcacgctgat cgtttcagga atcattgaag agaagaagca aatggttctg
    27061 gatgccatgt tagaacaagg gtttatcgtg gatcaaattt tccagcaaaa agactggtat
    27121 gcaattattt tgaaaaaacc agaggtggaa tagatgcagc ggtatttttt aaaagaatca
    27181 tatgaatcaa aagactattt tgttttagaa ggggaaaact atcaccacat tgtacgggtt
    27241 atgaggatgg aacccgagga tgaagttttt ttggcattca atgatcaaat agccattgtt
    27301 gcagaaatca cggagattac tgaagaggct gtttatctga aagaaattaa aaaagaagaa
    27361 aatcaaaagg aactgccaag ttcgattacc atagcgagtg gctatcctaa aggggaaaag
    27421 ctggattgga tcgttcagaa atccactgaa ttaggagcgc aagcttttct tggtttcccg
    27481 gcatcagcct ctgtcgttaa atgggatgca aaaaaacgta tgaaacgaca acaacggctt
    27541 gaaaagattg ctcaagaggc agcggaacaa tcccatcggc agatcgtgcc atcagtagag
    27601 ttattggaaa cgaaacaaga tttatttgag cggctagatg attatgacgt ggtcttggtt
    27661 gcttatgagg aatcagcaaa gcaaggcgaa aaatcaaatg cagccaaaat tcttagtgaa
    27721 acaaaaccag atagtcgaat cttagctgtc tttggaccag aaggtggatt atcccgcgat
    27781 gaagtcgatt attttgtaga acacaaaggg atactttgtg gattaggacc aagaatcctt
    27841 cgtacagaaa cagcacctct ttatttatta agtgctgcta gctatcaatg ggaattaatg
    27901 aatccttctt tcagctaacg ttcttcatta ataaaagagt atcgacagaa agaattgaaa
    27961 aaatagtcaa caatttgtat aatagaaaca attatttata caatggatac gaaaaatcgt
    28021 ttgaaaaagc ttttaaaaag cgaacatttc cgtaaaaaaa cgtataactg cacggatttt
    28081 gggacaatta tcaaacagat atttgtcccg attcccgttt taccgaaaaa agaaaggggg
    28141 atcgattcct gtgacaaaag aagaaatttt aacgggaccc ggtgtcataa agctcgttag
    28201 tcaatacatg ggtcccgaac atgtagcatt tgttgagaaa gcatgcgaat acgcgactgc
    28261 tgcacatgac ggacagttta gaaaatcagg cgaaccctac attattcatc ctatccaagt
    28321 cgcaggtata ttagcagatt taaaaatgga tccccataca gtggctacag gcttcttaca
    28381 tgatgttgtt gaagatacag aaatcacttt agaagatctg agagaagaat ttggcgatga
    28441 cgttgctatg ttagtggacg gcgtaaccaa attagggaaa ataaaatata aatcccacga
    28501 agagcagcta gcagaaaacc accgaaagat gctgctagca atggctcaag atttacgagt
    28561 tatcatggtc aaattagctg atagactaca taacatgcgt acgttgaagc acctgcgaga
    28621 agataagcaa agaaggatcg ctcaggaaac tttagaaatc tatgcgcctc ttgcacatcg
    28681 tctagggatc agccggatca aatgggaatt agaagataca gcacttcgtt atctaaatcc
    28741 aaaacagtat taccgtatcg tccacttgat gcagacgaag agagaagaac gtgaaaaata
    28801 cgtaagtggt actgttgaag atattcgaat agcgacagaa gagctgggga tttttgcaga
    28861 aatctatgga cggccaaaac acatttattc aatctatcgt aaaatgaaag atcagaaaaa
    28921 gcagttcaac gaaatttatg acttgctagc tattcgagtg atcgtagatt caatcaagga
    28981 ttgttatgct gtattaggag caatccacac aaaatggaaa ccaatgcccg gcagatttaa
    29041 ggattatatt gccatgccaa aagctaatat gtaccaatct ttgcatacta ccgtgatcgg
    29101 tccggcagga aatccggtag aaattcaaat ccgaacacag gaaatgcatg aaatcgctga
    29161 attcggggtt gctgcacact gggcctataa agaaggaaaa aatgaaaaag tagaaccaga
    29221 tggtatgacg aaacaattaa gctggttcca tgagatactc gaacttcaag acgaaagcta
    29281 tgatgcttct gaatttatgg aaggcgtaaa aggagatatc tttagtgata aagtctacgt
    29341 cttcacacca aaaggagacg ttactgagtt accaaaagga tccggaccat tggactttgc
    29401 atacagtatc catacagata tcggtaacaa aaccactggt gcaaaagtaa atggcaaaat
    29461 ggtgcagctt gattacaaat tgaaaaacgg agatatcatt gagatcatga cttctccaaa
    29521 ttcatttggc ccaagtcgcg actggttgaa attagttgct actagcaaag caagaaataa
    29581 gatcaaacgt ttcttcaaag cccaagatcg agaagaaaat gtgatcaaag gccacgaatc
    29641 cgtggtcaaa tgtattacag atctaggatt tacgcctaaa gatattttga cgaagaacaa
    29701 actgcaagaa gcactcgatc gttttaatta tcaaacagaa gatgatctct atgcagctgt
    29761 agggtatgga gaagttagcc ccttgacgat ggccaatcgt ctgactgaaa aagaacgtaa
    29821 agaacaaaaa atcgagcagc aaaagcaaga agcagaagaa atcatgaatc agccgaaaaa
    29881 agaacctgac aaaatgaaag tacgtcatga aggtggcgtt gtcattcaag gggtagaaaa
    29941 cttactaatt cgtatcagtc gctgctgtaa tccgattcct ggtgatgata tcgttggtta
    30001 tatcactaaa ggcagaggga tatccattca tcgtcgagat tgtccgaatg ttcagcctga
    30061 caaaccaaat gtagcagaac gtttgattga agtcgaatgg gaagatacat cgaatacacg
    30121 aaaagagtat gatgcagatt tggaaattta cggctataat cgttcaggct tattgaatga
    30181 tgtacttcaa acagtcaatg cgctaacgaa aaatctcaac agcgttgaag cacggacgaa
    30241 taaagataaa atggcgacga tccatttgac ggttggtatc cagaatttat cccacctaaa
    30301 gagtatcgtg gataaaatca aagcagtacc tgatgtctac agtgtacgcc ggacgaatgg
    30361 ataggaggac agatatgaga gcagttgttc aacgggtaag taaagctgct gtgtcagtcg
    30421 atcagcaaga aattggaaaa attggctacg gcctacttgt tttattaggc attcatgaaa
    30481 ccgatacgca aaaagatgtg gactatctgg taaaaaaaat tgcacaaatg cgtatttttg
    30541 aagatgaaca aggaaaaatg aatattagtg tagaagatgt gggaggacaa attttaagta
    30601 tctctcaatt cacactgttt gcggatacga aaaaaggaaa tcgaccaagc tttgtggaag
    30661 ccgcacgacc agaaacagct attcctttat acgaatcatt caatgaagga atcagaagtc
    30721 gcggcatcac agtcgctacc ggacaattcg gagcagatat gtcggtttct ctcgtcaatg
    30781 atggacctgt aacgatcatt attgattcta aaaacaaata aagtaatctg gttttcacta
    30841 gtaaaaacta tgaaacacgt cgcttaagtt agactgataa actctaactt aagtggcttt
    30901 tttgtctgca aaaagcgctt cttttgtcag cagtcggaaa aaaagttaag cttaaattat
    30961 acaagttatt ttttatagga ggttgctgaa gatgaaagaa gtaccagtag tagatatcaa
    31021 aatcacagat ttaaaaaaat tgtatcaaaa aggatacaac atgttggagg agttacgcca
    31081 tgaagcggac gctcccgtcg tcaaagcaaa gattttcaat aaagaagcta tcacgatcta
    31141 cggatcatca gcagcaaaag tattttatga tccaaggaat ttcaaacgaa aaggtgctat
    31201 gccaaaatta gtattgaaga cactttttgg tcaaggtgga gtacagactt tggatggagc
    31261 tgctcatcac catcggaaaa atatttttat ggatctaatg acaccggaaa gaatggaaga
    31321 ttatcatcgt atcttagata agaatctgac acaagcactt gaagcacagc atggtcaatt
    31381 tgaactattt gatttaagta agatggtttt ctttacatct atttgcgagt gggcaggtat
    31441 caatttatca gcgatttcta aagatgaggt cgaaaaatta gcagaatatc aaatctctat
    31501 gatcagcgga acgtttactt ctccgattga tcacataaaa ggtgtcgaga atcgcaaaaa
    31561 atcagaaaaa tgggcgcaag gcttgattga agaagctcga caaaatcctg tggctggaaa
    31621 agaaaatgtg gcattatatg cttttgccaa tgccacagat ttagatggtc agcttttacc
    31681 tctggaagtg gcagctgttg aattattgaa catcatccgt ccaacggttg ctctaactgt
    31741 gtgggcggca ttgatgggac atgcactttt cagtagacca gatttgtatc agcaattaaa
    31801 aaatgacttt tcgacgttac aagatccgtt tattcaagaa atgagacgct attatccttt
    31861 cttcccaatg ctacctgcga tttcgctgaa agaagtagaa gtagatggtt atcgtatacc
    31921 agaaggcagt tgggttattt tagatttata tggaacagac catgatgaac gaacagttga
    31981 agcgccagat tcctttatga tcaaacggta tgtagggaaa gcaaaagata tttcctataa
    32041 agaagaatat gagatgattg cacaaggcgg gggaaatttt cgacaaatgc atcgctgtgc
    32101 aggagaatgg atcactcttc atagtctacg tgtgttttcc gatcagctag taaataagtt
    32161 tgagttctct gtacctgaac aagattggac gattccattc aatcagtttc cgacttatcc
    32221 gaatagccga gcgctacttt acaagaatta gattagcata aacttatcga aaaaaaggaa
    32281 gatcaaacag tgctgatctt ccttttttca tattactata aattgataag gtcaattgat
    32341 caaaatccac gaaatgcttc atcaatggtt tgataatctt cattggataa atgcaaatct
    32401 aaagcttgga cattgtcaga tacttgctct ggacgcttag ctccaggtat aactacagac
    32461 actcgtggat tcttcatgta ccaagctaaa acaagttgcg caggagtggc ttggtatctt
    32521 ttagcaatgg ggcgaagtac atcgacagcg gttaaaattt ctctgaaacg ttctccttgg
    32581 aaatctggat ttctttttcg aggatctcct tcaccaaatt gtttttcctc accaagttca
    32641 tattttcctg tcagcaaacc ggaagcaaga ggaaaatagg gaacaaaact gattttgttt
    32701 ttttcaagat aagggaataa ttctttttca gcctgccgat ggatcaaact gtatttatct
    32761 tctactacat cgacatatcc atctgcattc gcttctttca attgttctaa cgtgaagttt
    32821 gacacaccga ctgctcggat ttttcctgct tcttttaatt cgtgcaatgt agcaactgat
    32881 tcattttttg gtgtgctttc atccggaaag tggatataaa aaatgtcgat gtaatcagtt
    32941 tgcagacgtt ttagtgcatc ttccaccgat tgtttgagaa actcaggaga attatcaaat
    33001 gttcgccctt tatttggtac atgagcagct tttgtagcga tgacgacacg tgagcgatca
    33061 tattcttttt ccttcagtac ctcgccaatc agttcttcgg aacggccgtt tccgtaagca
    33121 taagcggtat cgatcaaatt gatcccgcta ttcaaagctg tacgaactac ttgttttcct
    33181 gtctcatctt ccaaaccgtc aaaaagatta tgtcctccaa cggcatttgc tcctaatcca
    33241 agctgttctg cgtagacttg tgaatgtccg attcttattt ctgccatagc tgattcctcc
    33301 tttttgtcta gtctaaaaca aaataccaaa aagctgaact aaaacgtttt tgtgagggga
    33361 aatagtcaga tatctcttga ctttgagact ggtttttagt aggttataag taatatcgaa
    33421 tgtcaatgaa agagcgagta agtttttgaa actttacaag agaggatcat cgaggctgaa
    33481 aatgatctaa agtttcatta gcctaaagac actctggaga tggacgagtg aaaagccgtc
    33541 ccgcgtacga gcgttattcg taagaggaaa gagaaatctt tcaaaattag gtggtaccgt
    33601 gtatgaattt cattcaccct tgttcatgat gaacaagggt gattttttta tttacatagc
    33661 taagaaaggg agagaaaact atgagtttcc aaagaccaaa aggaacaaat gatattttgc
    33721 caggcgaatc agaaaaatgg cagtttgtcg aagaaacagc acgtttatta ttcagagatt
    33781 atcagtataa tgaaatgcgt acaccgattt ttgaacacta tgaagtgata tcccgaagtg
    33841 taggggatac aacggatatc gtttcaaaag agatgtatga tttttatgac aaaggggaac
    33901 gccatgtgac gcttcgacca gaaggtacag caccaatcgt tcgttctttt gttgagcata
    33961 aattgttcgg cccagagtat gcgaaaccgt tcaaaacttt ttatatggga ccaatgtttc
    34021 gctacgaacg ccctcaaaaa ggacggttac gtcaattcca tcaaatcgga gtagaagcat
    34081 ttggatcaga aaaccctgca actgatgtgg agagcatggt gatggcactt gatttcttca
    34141 aacagttggg gatcacgcaa atccggctag tcatcaattc attaggtgat aaagaaacgc
    34201 gtcaagccta tcgtcaagca ttgatcgact atcttttacc ttttgaatca gagctgagtg
    34261 aagactctaa acgccgcctt catgaaaatc cattacgcgt attggacagt aaagacaaac
    34321 gagatcaaaa atttgtggca gaagctccat ctatcttaga ttatctaagc gagcctgctc
    34381 aaaaacattt tgataccgtt gtttccatgc tggatgcatt ggctgttcct tatgaaatcg
    34441 acagtaatat ggttcgcgga ctagattatt acacacatac gatttttgaa atcatgagtg
    34501 acgcaccaaa aatgggggca caagcaacga tttgcgcagg cggacgttat gacaatctag
    34561 tggaagaact aggtggtcct tcaacacctg gcttcggatt tgcaatgggt atcgagcgat
    34621 tactcttgac tatggaggca gaggaagtag ttatccctgc atttaatgag ttggatgctt
    34681 acgtggtcgc actcggagac gaaacaaata tcgaagcatt gaaagtggtc caagcgattc
    34741 gaaactttgg tttttcagct gaccgcgact tcatgaaccg taaagcaaaa gcgcaattta
    34801 agacagcaga taaagcgaat gccaaattag tattggtgat cggtgaagat gagcttgcta
    34861 gtcaaacgat taatgtcaaa gcaatggcaa gccgaaaaga aaaaagtttt ccattagaag
    34921 agatatatga acattttgat gaagtctatg atgaaatgac tttattcgag taggaggaaa
    34981 aaaataaatg gcacaaagaa cgatctattg cggattagtt tccgaacaac aattagaaca
    35041 aacagttaca ttaaaaggat gggtacaaaa aagacgagac cttgggggag taatctttat
    35101 cgacctacgt gatcgtgaag gaatcgttca aatcgtcttt aatcctgaaa tcaacaaaga
    35161 agcatgggaa atcgcggata aatgtcgcag tgaatacgtg atcgaagtga ctggggaagt
    35221 tagaagacgc ggagaaaatg cagtaaatcc taaaatggcc actggtgaat tcgaagtgat
    35281 tgcaagtgaa atcgtgattt tgaatcaagc aaaaaccccg ccatttcaaa ttgaagatga
    35341 tcaagcaatc agtgacgaaa tacgtttgaa atatcgttat ttggatctgc gtcgtccaaa
    35401 aatgactaaa aacatcaaat taagaaatgc gacaacaaaa gcgattcgtc attatttaga
    35461 tgataatgac tttctcgata tcgaaacacc ttacctaggg aaatctacac cagaaggcgc
    35521 aagagactac ctagttcctt cacgtgtcca tgctggacat ttctatgcgt tgccgcaatc
    35581 tcctcaattg tttaaacaat tgctgatgaa tgctggattc gaccgttatt atcaaatcgt
    35641 tcgttgtttc cgcgatgaag atttacgtgg agaccgtcag cctgaattta cgcaagtaga
    35701 tattgaaaca acgttcttaa cagcagaaga aatccaaact tacacagagg gattgattgc
    35761 gaaggtaatg aaagaagttc gcggtatcga agtcacattg ccatttcctc gtatgaccta
    35821 tgatgaagca atggctcgct acggcagcga caaaccggat acacgttttg atatggaatt
    35881 gatcgatcta agcgatacag tcaaagaagt agaattcaaa gtcttccaaa tggctcttga
    35941 aaacggcggt gtcgtgaaag cattgaatgc taaaggcgct gcagatcgct actcacgaaa
    36001 agacatggat cagttaggtc aatatgtagg acaattcgga gctaaggggc ttgcatggct
    36061 gaaagtagaa gaagacggat tgaaaggtcc aattgctaaa ttcatgggag aagcaacaga
    36121 agcaatcatt aaagcaacag atgcaaaacc aggtgacttg ctgatgtttg gcgcagataa
    36181 aagtgaaatc gtcgctgctg cattaggtgc tatccggaca cgtttaggaa aagaattagg
    36241 actaattgat gaatcgaaat tcaatttctt atgggtaact gattggccac aatttgaatt
    36301 ttcagaagaa gaaggtcgat atgtctctgc tcaccatcca ttcacgatgc caaaagaaga
    36361 agatatcccg ttattggctg aagaccctgc aaaagtctat gcagaagctt atgatatcgt
    36421 cttgaacggt tatgaattag gcggaggttc tatccgtatt catactcgtg agcttcaaga
    36481 aaaagtacta gaaactctag gctttacaaa agaacaaatg gaagaacaat ttggtttctt
    36541 gttaacagct ttagattacg gattccctcc acatggcggt attgcattag gattggaccg
    36601 ttttgcaatg ttattagccg gagaagataa tattcgtgaa gtcattgcct ttcctaaaaa
    36661 cggaaaagca gcagatgtga tgagtgatgc accaagtatg gtttcgcctt tacaattgtt
    36721 cgaattgaat attgatgtaa cgaatataga agaatagccg ataaaaaggt aaaaaggatt
    36781 tgtcgaatac gcttcttttg acaaccttga tttaattgaa aaacacttta taatgaagcc
    36841 ataacatata aagcggggct tttccatgaa aaaaacattc gaaaattggg cttatcatga
    36901 ttacacgacc aagctttcgg tatctatcgt ttatgcgttg atcgcatcag tcgcattgaa
    36961 tttcttttat cagccaggac atatttattc aagtggtatt actggtctag ctcagatttt
    37021 gacgactttg tcaaaatcac tgataggatt cgaagtaccg gtatcaacaa ctttgtattt
    37081 actgaatata cctttgtttg tattggcgtg gttaaaaatc gggaagaagt ttacaatttt
    37141 cacttttatc acagtcactt tgacctccct ttgtatccat attttaccgg aaacagtcct
    37201 atctaccgat ccaatcatct gtgcgttgtt tgggggggct gtgatgggac taggtattgg
    37261 gctagcgttg agaaacggcc tatcatcggg cggtctagat atcgtgagta tcacgattcg
    37321 taaaaaaaca ggaaagtcga ttggttctat ttcgatctac ttcaatgcat tgatcgtttt
    37381 tgttgccgga attctatttg gctggcaata tatgttttac agtacgttgt ctattttcgt
    37441 cagtggaaaa gtaactgatg tcgtttatac taaacaaaag aagatgcagg taatgatcgt
    37501 aacgaaacat ccagaaaaag tagtaaatga aatccaaaac tgtatgcgta gagggatcac
    37561 gatcatcaat gaagcagaag gtgcttataa gcatgataag cagacagtat tgatgacgat
    37621 cgttacacga tacgaactgc ctctacttag agaagcaatg aaagcttctg atccacaagc
    37681 ttttgtaagt attgcagaaa acgtccagat acttggtcgt ttctatgagg atggcattta
    37741 gatcgagttt gacaaaaatt catgaaatac tgctgtagac agacgttcgt ttgtctgcag
    37801 tttttttcta ccatagtaga atcagcaaag aaaaactgct tttttatttg aaattttctt
    37861 aaagttgaaa aaaagcatgt ttattttttt aaatttatgg gatgatagaa aaaaatagta
    37921 gagaaaggac acgattctac ttgctatttt cacttaatac ccgtatatta cgaatgaaaa
    37981 aagtttatga aaagttggaa tattgaatgg aaaaacaaaa aaggttaaaa aacagccgct
    38041 acatcactgg gtttgatggg ctccgttcat tagctgtggt gggtgttatc ttctatcacc
    38101 ttctgccaac tagtatgaaa ggcggctatt tgggcgttcc gatttttttc gtcgtatctg
    38161 gttatttgat cacggatctg ctgcggcagg aatgggaaca aaatggaaaa atcaatattt
    38221 ggcaatttta tgtcagaaga atgaagagac tctatccggc acttgttttt atgcttgtga
    38281 cagcttcggc ttatatcact ttgtttcagc gagggctgtt aaacaatctt cgaggagttg
    38341 tggtcagcag cttactgtat gtcaacaact ggtggcaaat caataacggt ttatcttatt
    38401 tcgatcgatt tgctaatgag tcaccgttta cgcatatctg gtctttagca gtagaaggtc
    38461 aaaactattt ggtttggccg atcttgtttg tattgttgat ggtatttgtt cgtaaaagaa
    38521 aatggatctt ttatactgtg ttaggtacat ccttcctctc cgccatcttg atgatgatct
    38581 tgtatacacc aggaggcgat ccaacccgtg tttattacgg taccgacact cgtctgtttt
    38641 ctatatggtt gggaagtgcg cttgcgtttg tttggccaag tacgcgttta aagaaaaaca
    38701 tcccgaaaca agcgaaaaat attctgaata tcgctggagg aatctcgctt ttggcactga
    38761 ttctttgctt cttcttttta gatgatcatt acaatttcat ctattacgga ggaatgctcc
    38821 tagtgagtct gttatgcgta gtgctagttg cagtgacagc acaccccgga gcaagtttga
    38881 atcgatggct gacaaatcca gtatttactt ggattggcaa acgaagctat ggcatttatc
    38941 tgtatcagtt tccagttatg attttttatg aagcaaaggt gactgatatt gccgatcatg
    39001 ttttgttgca tactttagtg gaaatcacat tgattctaat catcagtgaa ctttcttatc
    39061 gatatgtgga gcgtccgcta gcacgttttg attatggtcg tacattccaa gtgatgaaag
    39121 gatggttcag caaaccgatc atcagtaaga gcaaaccatg ggttgttcca ggcacattac
    39181 ttgtagcaat tgcactcgtt ggtttcgtta cggcaccgaa aaacgctgta acagccgatc
    39241 aaaaacaact gcaggcaaaa atcgctgaaa gtaaaaaatt agcagaagaa accagaaaca
    39301 attcagaaaa caccactagt tcggttgacc aagcgatcct tgataagtac gagttgtcca
    39361 aggcacaagg gaataaggca caaaaattag aactaacggc atttggtgat tcagttatgt
    39421 tggatgctgc aacggatcta caagaacttt atccaaaagt agtggtagat ggagatgtcg
    39481 gacgacaatt atatgccagt gtaccgtaca ttgaaaaatt aaaggaacaa gatttgttga
    39541 aagacactgt tctgattgga ctaggaacga atggctcatt ctcggaagct cagtttgata
    39601 gtgtgatgga tgcaatcggt gatcgaaaag tatactgggt caatgtccga gtaccgacga
    39661 agcgctggca aaatgacgtg aatgcgatgc ttgaacagat gacgaaaaaa tacgacaata
    39721 tgacgctgat tgattggtac agctacagta atgaccatag tgactggttt tatgatgatc
    39781 aagtccatcc aaatcctgaa gggatgacac attacattca cttagtcagc gaagccttgc
    39841 taggaggaag ttcaagtacg aacagcagtg agacaaacgt gacagaatga tagctacaaa
    39901 aaacttcagg caaaaacatt tgtttttgcc tgaagttttt ttatatttac cgtgttttta
    39961 acattcatat agattcagga agcaaaacgg ttgagtgtgt ctgtaaataa cgttcttttt
    40021 gttttcgggt gagttttttg aatgcagctt ctgcttttgt tgcttcgctt cttgaagaaa
    40081 atacttctgc atggatcatc ttaacaggtc ttcttttttg aagacgagta tatttagcac
    40141 cagtaccatt gttatgttcc tctaatcgtc tagaaagatc ggttgtatat ccaccataaa
    40201 atgtctgatc tttacagaat aatacgtaaa aataatgttc atttgccata gagcatctca
    40261 cgaatctctg cagtgtattc tccatccata ttgtaagtga tcaatggtgg agcgactttg
    40321 aatccgtcta atttgccttc tttgattcct tcaactaaca aaatatttgc ctcttttcca
    40381 ggttttgggt aaacaaattg gatcctttta ggtgcaattc gatagtcttc catgacctga
    40441 atgatttcta agaaccgatc aggacgatgg accatagtaa agcgtccatt tgtttttagt
    40501 agcttacttg aaatatcgat cacttcttct aacgtcgtat gaatctcatg acgggcgatt
    40561 gccaggtgtt tattagggtt cgttttattt gttggcagcc ctttgaaata aggtggatta
    40621 cagacgacta agtcacaaga attatgtcga acgacagata agctgtcttt cagatcgatg
    40681 gtgtggacag tcatttgttc atttaaatgg ttaagcgcga tactacgtct tgccatgtct
    40741 gctagacgag gttgaagttc gatagcgtcg atagccgcct gggttcgttt acttaaaaaa
    40801 agtccaactg cgccatttcc tgcacaaagg tcaacaatct tccctttttt aggtacacgg
    40861 gaaaagtgag caagcagcac agcatctaaa gaaaaagaaa acactttgga actttggata
    40921 atcttgatat cttctgcgta taattggtcg attcgttcgt cttcgtgaag catggattcc
    40981 ctccaaaatg aaaaaaatag tcgtctgtat tatactattt ttattaaaaa gaaccaaggt
    41041 ggcttgtcaa acctctattt tttatgcata ctatgaccat aagaagggaa aggaagagac
    41101 tatgttttat cgatttatgc gcggggtagt tcgagtacta ttagccttag ttaatggaaa
    41161 tgcccgctat gagaataaag aagttttacc gcaagatgag aattacatac tagttgctcc
    41221 ccatcgtact tggtgggacc cattatatct ggcggtagct gcagcaccca aggaattcag
    41281 ctttatggca aaagaagaac tgttcaaaaa tccagttctg cgatttatat taactcgatc
    41341 caatgctttt ccagttaaaa gagaaaatcc aggaccaagc gtcattaaaa cacctgtaaa
    41401 gattttgaaa tcgacaaatt tgagtttgat catgtttccc agtgggacaa gacatgctac
    41461 agaacttaaa ggcggaatgg cattgatttc cagaatgtct aaagcgaaaa tcgtgccagc
    41521 tgtatatcaa ggtccgttga cacttgggga tctgttcaag cgtcagagag tgacagtgcg
    41581 tttcggcgaa ccaatcgact tatcagatat caagaaaacg gataaagaag gtatggcgga
    41641 gatcgaacga cgaacacaag cagcttttga caaattggac aaagaagtga accctgattt
    41701 caaatatgaa gtcaaaaaaa aataaaatga taagtttatg cgtgggcggt cttttgaatc
    41761 tactgtcctg ttcagttcag actttttcaa tgaaaacgag ggagagacgt caagtctgcc
    41821 ctctttttgc gttccaatat gtaaaataga agacaggtgt catgtaatgg atcaaagtgt
    41881 tataataatt agaaggacat tttttgaggt gattgaatgc gttttttaca tacagccgat
    41941 tggcatatcg gaaaaaaact acaaggttat gacttgattg aagaccagcg tcatgtacta
    42001 tcttctatac tagatatcgc gaaagaagaa aaagtagagg caatcgtgat tgccggagac
    42061 ttatatgata ggagtgtccc agctgttgat gccgtagaat tatacaatga attgatggtc
    42121 gattggaatt taaaggaaaa gatcccgatt tttgctatat caggaaatca cgatagcagt
    42181 acgagacttt cagcgggtac gccatggttc agttattcga actatttttt gcgaacacag
    42241 ctagcagaag catttgaacc agtcgagttt aaaaacgtac aatttttctt tcttccttac
    42301 tttgaaccaa ttgcagcgag attatatttt gaagatgaca atatccgaac gatcaagcaa
    42361 gcggtagcca aggtgataga taaaatgcag gagctatttg attcagaaaa aaaacaagta
    42421 ttgattagcc atttttttgt aatgggaagt gtaaaatctg attcggaaac gaaaatcgaa
    42481 gtaggcgggt tggattcgat ccctggtgaa ctacttacac cgtttgatta tgtagcacta
    42541 ggacatttgc ataatcaagc tgctttgaga caggataatg ctcgctatag cggttcccca
    42601 ttgaaattct ctttatctga gatcaaccaa aaaaaaggcg tatggatcgt tgacctgaca
    42661 acaggcctgg agttgaattt tagggagatc gaacccctgc atgatattga agaaataaca
    42721 gcgagtttca aagaattgct ggatcctgcc ttttatcagt ccgtcaatcg ggagaattac
    42781 atctatatct atctcactga tagaacgatc atcccgaata tgatgaacca gcttcggcaa
    42841 atatatcctc gtattttggg gattgaacgg gcaaacggtc gagcagaaca aacaaagcgc
    42901 aaacgaaaag tactagaaat gaaagatcct caaaaattag cagaagcatt ttttagtgaa
    42961 atgacggaag agcagctaac tgcaaaccaa gaaaaatggc tcggagaaac gttgcaagaa
    43021 atcatggaaa tggaatgatc aagaatgaaa ccgaaaaaat tacaacttaa aaattttggt
    43081 ccttttatca atgagaccgt agatttttca cgtttgacag aagcaccgct ttttttgatc
    43141 agcgggaaga caggagctgg caaaacgacg atttttgatg ggatcacctt cgcacttttt
    43201 ggtgaaactt ccggaagact tcgctctgga aaagagatgc gttctttgtt tgctacacca
    43261 gacgaagaaa caagcgtgac tttttctttt gaacatcagc agatgagcta tgaaatcgaa
    43321 cgaaaaccag aacaagtttt agcaaaacga aaaggaaacg gaacaaaaaa acaggcagcc
    43381 aaagtaacgt tgaccatttt tgatgggaaa ggaaaagaaa taaaacaaat cacaaaacgt
    43441 acagaagcag atcagttgat cagagaactg atgaatctcg atgcgaaaca gttttctcaa
    43501 atcgttttac taccccaagg agaattccgt aattttttgg tttcatcaag cagtgataaa
    43561 gagactgttt tacgtcattt attcggtact caatttttcc aacagttcaa tgagcgatta
    43621 aaagaaaaag caaaacaaca gcaacaaaaa ttggatcatt tagagcaaga gctgcatctt
    43681 ctgcaaaaaa ggtttgtttt agtcaaagaa gaggaaccag cagcagggtt tgaagccgtt
    43741 ttagcccaat ggacaaacca tcaggcaata ctcgaaaaac aaatcacgga agaaaaagaa
    43801 gctctaacgg aacttcagga aaaacaaaag caactagaaa attcctacta tcgttttgaa
    43861 agcttgaaaa aaagttatga agaaaaaacg atattaataa acaagcaaaa ggaattagct
    43921 gctcaaaaag acgaaataga ggaaaagaaa cgatggattg ggtattatga atttacgcaa
    43981 cgattagtcg aaccaatcca gcgcctacaa gaaataaaag ttgaaaaaat agaactgtca
    44041 caaaaaagta gtgagcaaac aaagaaacta gcgatgacta cgcagaatta tgaagagtgg
    44101 caaaaaaatg aagcaaaccg cgtacgccga caaacaaaaa tcaaagaagc tgctcaaaaa
    44161 ctgcaaaaca aacagatgat ggtgccaatt gtcgaatcct tacgtagtaa aaaacaagac
    44221 gcagaacaat taacaatttc attggataaa gaagaattga atgccgagaa gctgtatcaa
    44281 aatctcgcta tctatcagga aagaactcga attcttcaag aaaatttact ggatcaggaa
    44341 aaactgcaag aagatcgtct tgcttttgga cagttaaaat atctagatga aaagcagcaa
    44401 gaaatagaaa aacaaaaagc aaaagacact gggtcgctcg cacagataag agaagaactc
    44461 aaaaaagcaa ctgaagaaat aacaaaaaat agtcagctat tgaaagaaca gacagaacaa
    44521 gcagaacagc tgaaaagcca atgggcaaag cagcaaattg ctcgtcttca gctttttttg
    44581 cgtccagggg agccatgtct tgtttgtgga tcaacggaac accccataca acaattaaaa
    44641 cacgaacagc caaataagga agatatctta aaagtagaac aacaactggc agaagctgag
    44701 tctgctgtag aaagaactca gcaaatcatt gccaaaagtg aagcacatga aaataatctt
    44761 caacaacaat tatcagattt agaacaaaaa atcatggcaa cgaatataca attggctgaa
    44821 caagaagaac acttgcaaca ggctttgaaa aaggcatttc ctgagataac agaacaaaaa
    44881 acaaaaacaa ctcttgtagt aatcgaacaa tctttagata gacgaaaaag caagatggcc
    44941 aatgaaagaa tagagctgga agaaataaga caacaagcag aaaagctcga acaacaatta
    45001 gctgtctaca aacagcagca gacagaaaaa aataatcagc tccagcaact gcaaggagaa
    45061 atcaaaagtt tagaacaaca attgcaagat acggatagta actgtttaga agaggagata
    45121 cgacgacttg aaaaacagtt agaagaagac tcgaatcaac tagctgttga gcagaaagca
    45181 ggagtgcaat tgttgcaaga tcttcatacg cttcggacac agcttgatct tcaacatgaa
    45241 caaatagaaa aaatagatga aaaaatagaa aaggaacaag aaagaatagc taaaaagtta
    45301 gaaagccagt cgtatttttc aacaattgaa gaaatctgtg catttgcaca acagcaagca
    45361 caatataaag aaaaccaacg tacagttact tcttatcatg aagagcaatt agtgacagct
    45421 gaccggctgc gtcaattaga aaatatccag ctaactgaga cattcccaga tacagaagag
    45481 atcagccaac agttagaaga aataagacgt atgatcgaaa taaagcaaga aaagatatac
    45541 ggtttacaag aacaaaaaat tagtaatcaa aaaatctacg atgaatgtct ttcgatttat
    45601 cacacaagcc agacgaaatt agatgagctt acgcaattac agcagctttc gcaaacattc
    45661 aacggagaaa atcctaagaa gactagtttg gaacggtatg tgcttcaggt ttatcttcaa
    45721 gaagtgttac aagtagcaaa tgatcatctc caacgtttaa cgaaaaatcg ttatcaattt
    45781 gagctagcgg ataccattgg cagttatcga gggaaaaccg gattagaaat caatatctat
    45841 gatgatcagg caggtatgtc tcgaagtgcg cataccctgt ctggcggtga gagttttatt
    45901 gcagcacttt ctttagcatt agctcttgcc gatgtgatcc agacacaagc tggtggtgta
    45961 gcaatcgatg cattgtttat tgatgaagga tttgggtcat tagatgaaga agctttggaa
    46021 atggcaatgg aagctttaga aacgatcgaa agtgaaggaa gaatgatcgg catcattagt
    46081 catgtaagag aattgaaaga acgagtatta caacaaataa gaattgatac agaaggtagc
    46141 ggacaaagca aaatacgcta tcaacttgga taaaaaggga gattggtaac tatgaaatgg
    46201 agtattccag aaaaagtcat tgcacgtgga cgtcaatact tgcaagaagg ccgagtgtta
    46261 tcggttgtcc ctgaccaaga agaaaacgtg tggcatgcag aagtcttggg cagtgaattg
    46321 tatatggttg atttggatgg tacagcaaaa gagatagatt actgccaatg tccctattgg
    46381 gaggaacata aatattgtaa gcatacagtt gctgtggaac tttatttacg gtcgaaaaat
    46441 ctatctcgta tcatgaaaaa agaccagcca atgctagaac cggtaaaagc aacaagtgag
    46501 ggagaaatca ttgcaaaagg tttttctcgt ctaaaaacac aaaacaatcc tcaagcggtg
    46561 caacctttga ttattgaata ccaagtagaa acgattgaga ctaatcagta tcacccagag
    46621 ctttctattt tggcacttta cttgcgtatc ggttcattgg catcaccgaa aaaaacatat
    46681 atcgtaaaaa acatctatga ttttttacaa gcttacacag aaaaagaaac ttacactgtt
    46741 aataaacagt accagtttcg tctagataaa cgggcgtttc aaaaagaaga tcaaaaaata
    46801 ctcacgcatc ttgcaggaag tgctcagacg catcaattat taggtacgaa cggcttacaa
    46861 gtcaaaggaa aactagataa aagatatctg cttctcccag tagaacaagc ccgcttcctg
    46921 ttagagcaga tgaatcagac tacacgtttg catctgcaga taaatgaaca aaacgatcaa
    46981 ggaatacaat tttctgatca aaacaaacct ttatcattca aagtggaaaa atttggagaa
    47041 gattatttgc tgcaagcatt gaatgatttt gattttttct ttatgcatta tcagtgggga
    47101 gttatccaag ggacatttta ttctctgacg aaatatcaac aaacggttta tcaaacttgg
    47161 aaacaattga ttcgccgatt ggagaaacca gaagtagtgt tcaagaaaaa agaattaccg
    47221 tctttgttta aagagattat ccctctgctg tcagagatcg gcgacgtagc tattgcccct
    47281 gcagtagaag aggaagtagc agatatcccc gtcgaattta ttttcttttt ccgaaaagca
    47341 aaaggaaaaa tccaagctcg agtcgatttc gtctatggtg aagtcatcta ttcgactgac
    47401 ccgaagcatg aggtctcgac agaacaggct tttcaggtgt tacgtgacac agtagaagag
    47461 aaacgagtac ttgatctatt cagaatgtat cgctaccaga aaaatgaaac tggttatgaa
    47521 cgtccattgc caagcgggga agaactatat gcctttttcc gcacagaact atctgtcttc
    47581 cgtcagtttg gtgaagtcag acttggaaga aaattgcgag agttgtattt agatgcgcat
    47641 aaacatcaac cgaaaatcga agtaaccgag accggttcat ggctcgatat tcgattcgat
    47701 gtgacaggga tagaggagca agaaatcgat catgtcctgc aaagcttgct gagaaatgat
    47761 gctttttata cattagaaaa tggtcaggtt ctttcttttg attcagaaga atttcaacaa
    47821 acaagccaag tgttgcaaca attacgggaa tcgatacgta cagaagaagg aacgatccat
    47881 gtacctaaaa atcaaggatt gatcattcaa aatcaattgg aaaaaagcaa tgctactttt
    47941 agtgaatcgt tccaaacgat ggtacaagat ctcattcatc cagaaagata ccaggcacag
    48001 cttcccaaag gattgaatgc aacgatgaga gattatcaaa agcaaggttt tcgatggttg
    48061 aagatgttag gtcattatca gtttggcggt attttagccg atgaaatggg acttgggaaa
    48121 acactacaga caattgcctt tttactttcg gaaaaagaag aacggaaaag cttctctgct
    48181 ttgatcgtag cgcctgcgag cttaatttac aactggcagg cagaggtgag aaaattcgcc
    48241 ccttctttaa gtatccaagt aatcaatggg aataaaaaag aacgtgaaga attactggct
    48301 aaagacacag atatccgagt gacttcttat gccagcttaa gacaggatct agctaattat
    48361 cagtcgcaaa aaattgatta tttgattctt gatgaggcac aaatggtcaa aaatagcagt
    48421 acgaaaactg cacaagcatt gagagaatta gctgtacctc agcggtttgc actgagtggg
    48481 acaccaatcg agaataattt agaagaattg tggtcattgt ttgcaacgat tatgccagga
    48541 tttttcccga ctaaaacaaa attcagggaa cttgccgcag aagaaattgc gcaaatgatt
    48601 cgaccgttta ttttacggcg agataaacaa actgtgttga aagacttgcc ggaaaaaaca
    48661 gagatgaatc tatattctgc acttacagaa gaacaaaaaa ctgtttattt agcttacttg
    48721 cgtcaaatgc gtgaagaaat cacatcaatg gattctgaag cattcaagaa aaaccggatc
    48781 gggatacttg ctggtctgac aagactgcga caaatttgct gcgatcctcg tctgtttatt
    48841 gaagattacc aaggcggttc cggcaagctc gaacaagtaa aagatctact ggtagctgcc
    48901 aaagagaaca aacgaagagt tttgctgttt tctcaattta cagggatgct gactatcttg
    48961 caagaagagt tagccgagct agggatctcg acattttatt taagaggcag tacgaaacca
    49021 caagatcggt taagtatggt agacgcattt aatgctggcg aaaaagatgt ttttctgatt
    49081 tccttaaagg ctgggggaac tggattgaat ctcacgggag cagatacggt catcttgtat
    49141 gacctttggt ggaatcctgc cattgaagaa caagctgctg gacgtgcaca tcggatcgga
    49201 caaaaaaatg tggtagaagt gtggcgtatg atctctgaag gaacgattga ggaacgcatg
    49261 gattcactcc agcaagaaaa acgcgaactg ttccaaaaag tgatccaagg taatgaagaa
    49321 cagttgacga aactgactga agaagacatc cgcatgattt taagtgtagg agaaatcgag
    49381 gagtaagaga ctcgaaaaag cgttcagtaa tgggtaagaa agcctttcta aagaagcgat
    49441 tgtttatttg atttaaaaaa tagcaaggag gctgggattc cattttatcc cagcttcctt
    49501 gctatcattt ttttatttct atttttaaag gttatacaaa gattgtcttc attgtctcta
    49561 acagctgatc aaaagaagaa atctcgatat cgcaaattgg agtctgttca attttgcgtt
    49621 ctcgatggtt gaaccaaagc gcttgccagt tagcactttt tgcacctaga acatcattgt
    49681 cgtaattatc gccaacgtat aaggtctctt ctggaagcat atgaaattct ttctcagcca
    49741 gctgaaaaat ctctttttct ggtttttgga aacctgttgc ttgagaaatg agcatattgc
    49801 gggaaggaat ccaattgttc aattgaagtt gcttcagttt ttttgtctga tggtcagtcg
    49861 gcccatttgt gataattcct aatgggacat tcttttcctt caagaaatcg agggtttttt
    49921 tgacctcctc atgcaagcag atattatcaa gttcttcctc gtaaattttt tgaaataaga
    49981 ggccatcttc ttccgtaata tgagggtagt ctaaatcttt tagtgattgg ctgatccgat
    50041 gtgctcgcat atattctagt gtccaatctc ctgccattac ttttgggaag ttttcatcac
    50101 tatgatggcg gaaccggata tatagtggat gcatatctgc atctgaaaca agcggaacga
    50161 ctcttttgac cgcgtttcga aatggctgct gctgatcata catcgtatca tcaacatcaa
    50221 acacaactga tttaatcaaa attttacacg cctttcggtt ttcattgatt ttttcacaaa
    50281 tccttgacta ttgtaacaga aaaaattgtc taaaaaagct gataaataaa ggtttttctt
    50341 actatatttc tatttccttg acagaataag gtttttatat ttcagttttt ttcactaaaa
    50401 atcgtgaaac acgtcaataa gtttgatgaa caaattaatc attatggtag aagtcgattg
    50461 atttatttta atgcaatctc tgtggaattt tcgaaagatt tattttttta atacagtctc
    50521 aatataacta ttcaaacata aaataacgtt atttgattta tttgtttata gtaaatttta
    50581 tttagtaatc attgctttaa aatactacg
//
LOCUS       DACDTO010000012        48921 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDTO010000012 DACDTO010000000
VERSION     DACDTO010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.012"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            226..1200
                     /locus_tag="IWK70_000733"
     CDS             226..1200
                     /locus_tag="IWK70_000733"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9613301.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            1370..2770
                     /locus_tag="IWK70_000734"
     CDS             1370..2770
                     /locus_tag="IWK70_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9613302.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(2848..3570)
                     /locus_tag="IWK70_000735"
     CDS             complement(2848..3570)
                     /locus_tag="IWK70_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9613303.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(3649..4155)
                     /locus_tag="IWK70_000736"
     CDS             complement(3649..4155)
                     /locus_tag="IWK70_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9613304.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            4278..6077
                     /locus_tag="IWK70_000737"
     CDS             4278..6077
                     /locus_tag="IWK70_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9613305.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(6145..7719)
                     /locus_tag="IWK70_000738"
     CDS             complement(6145..7719)
                     /locus_tag="IWK70_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613306.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(7974..8483)
                     /gene="mreD"
                     /locus_tag="IWK70_000739"
     CDS             complement(7974..8483)
                     /gene="mreD"
                     /locus_tag="IWK70_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9613307.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(8487..9341)
                     /gene="mreC"
                     /locus_tag="IWK70_000740"
     CDS             complement(8487..9341)
                     /gene="mreC"
                     /locus_tag="IWK70_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9613308.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(9496..10134)
                     /locus_tag="IWK70_000741"
     CDS             complement(9496..10134)
                     /locus_tag="IWK70_000741"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9613309.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(10135..10785)
                     /locus_tag="IWK70_000742"
     CDS             complement(10135..10785)
                     /locus_tag="IWK70_000742"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9613310.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(10798..11697)
                     /gene="rsgA"
                     /locus_tag="IWK70_000743"
     CDS             complement(10798..11697)
                     /gene="rsgA"
                     /locus_tag="IWK70_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9613311.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(11862..13931)
                     /gene="pknB"
                     /locus_tag="IWK70_000744"
     CDS             complement(11862..13931)
                     /gene="pknB"
                     /locus_tag="IWK70_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9613312.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(13928..14668)
                     /locus_tag="IWK70_000745"
     CDS             complement(13928..14668)
                     /locus_tag="IWK70_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9613313.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(14681..16039)
                     /gene="rsmB"
                     /locus_tag="IWK70_000746"
     CDS             complement(14681..16039)
                     /gene="rsmB"
                     /locus_tag="IWK70_000746"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9613314.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(16039..16977)
                     /locus_tag="IWK70_000747"
     CDS             complement(16039..16977)
                     /locus_tag="IWK70_000747"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9613315.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(16970..17461)
                     /gene="def"
                     /locus_tag="IWK70_000748"
     CDS             complement(16970..17461)
                     /gene="def"
                     /locus_tag="IWK70_000748"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9613316.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(17476..19884)
                     /gene="priA"
                     /locus_tag="IWK70_000749"
     CDS             complement(17476..19884)
                     /gene="priA"
                     /locus_tag="IWK70_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9613317.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(20137..20442)
                     /locus_tag="IWK70_000750"
     CDS             complement(20137..20442)
                     /locus_tag="IWK70_000750"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9613318.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(20447..21061)
                     /gene="gmk"
                     /locus_tag="IWK70_000751"
     CDS             complement(20447..21061)
                     /gene="gmk"
                     /locus_tag="IWK70_000751"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9613319.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(21548..21832)
                     /locus_tag="IWK70_000752"
     CDS             complement(21548..21832)
                     /locus_tag="IWK70_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613320.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(21850..22740)
                     /locus_tag="IWK70_000753"
     CDS             complement(21850..22740)
                     /locus_tag="IWK70_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9613321.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(22760..22978)
                     /locus_tag="IWK70_000754"
     CDS             complement(22760..22978)
                     /locus_tag="IWK70_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613322.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(22948..24648)
                     /locus_tag="IWK70_000755"
     CDS             complement(22948..24648)
                     /locus_tag="IWK70_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9613323.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            24915..25688
                     /locus_tag="IWK70_000756"
     CDS             24915..25688
                     /locus_tag="IWK70_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613324.1"
                     /translation="MTESVYVHIDTTSNAVSTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(25769..26338)
                     /gene="thiT"
                     /locus_tag="IWK70_000757"
     CDS             complement(25769..26338)
                     /gene="thiT"
                     /locus_tag="IWK70_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9613325.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(26404..26506)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(26512..27483)
                     /locus_tag="IWK70_000758"
     CDS             complement(26512..27483)
                     /locus_tag="IWK70_000758"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9613326.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(27577..28950)
                     /gene="glmU"
                     /locus_tag="IWK70_000759"
     CDS             complement(27577..28950)
                     /gene="glmU"
                     /locus_tag="IWK70_000759"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9613327.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(29453..30271)
                     /gene="purR"
                     /locus_tag="IWK70_000760"
     CDS             complement(29453..30271)
                     /gene="purR"
                     /locus_tag="IWK70_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9613328.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(30438..31262)
                     /locus_tag="IWK70_000761"
     CDS             complement(30438..31262)
                     /locus_tag="IWK70_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9613329.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(31235..31915)
                     /locus_tag="IWK70_000762"
     CDS             complement(31235..31915)
                     /locus_tag="IWK70_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9613330.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(31938..32876)
                     /locus_tag="IWK70_000763"
     CDS             complement(31938..32876)
                     /locus_tag="IWK70_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9613331.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(33121..33972)
                     /locus_tag="IWK70_000764"
     CDS             complement(33121..33972)
                     /locus_tag="IWK70_000764"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9613332.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(34126..36699)
                     /gene="mprF"
                     /locus_tag="IWK70_000765"
     CDS             complement(34126..36699)
                     /gene="mprF"
                     /locus_tag="IWK70_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9613333.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            36840..37646
                     /gene="proC"
                     /locus_tag="IWK70_000766"
     CDS             36840..37646
                     /gene="proC"
                     /locus_tag="IWK70_000766"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9613334.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(37725..39281)
                     /gene="rny"
                     /locus_tag="IWK70_000767"
     CDS             complement(37725..39281)
                     /gene="rny"
                     /locus_tag="IWK70_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9613335.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(39692..40741)
                     /gene="recA"
                     /locus_tag="IWK70_000768"
     CDS             complement(39692..40741)
                     /gene="recA"
                     /locus_tag="IWK70_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9613336.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(40850..42091)
                     /locus_tag="IWK70_000769"
     CDS             complement(40850..42091)
                     /locus_tag="IWK70_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9613337.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(42331..42909)
                     /gene="pgsA"
                     /locus_tag="IWK70_000770"
     CDS             complement(42331..42909)
                     /gene="pgsA"
                     /locus_tag="IWK70_000770"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9613338.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(43118..44002)
                     /locus_tag="IWK70_000771"
     CDS             complement(43118..44002)
                     /locus_tag="IWK70_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9613339.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(44780..46066)
                     /locus_tag="IWK70_000772"
     CDS             complement(44780..46066)
                     /locus_tag="IWK70_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9613340.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(46059..47327)
                     /locus_tag="IWK70_000773"
     CDS             complement(46059..47327)
                     /locus_tag="IWK70_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9613341.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            47579..48040
                     /gene="mscL"
                     /locus_tag="IWK70_000774"
     CDS             47579..48040
                     /gene="mscL"
                     /locus_tag="IWK70_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9613342.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(48100..48546)
                     /locus_tag="IWK70_000775"
     CDS             complement(48100..48546)
                     /locus_tag="IWK70_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9613343.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(48639..48884)
                     /locus_tag="IWK70_000776"
     CDS             complement(48639..48884)
                     /locus_tag="IWK70_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613344.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
BASE COUNT        13791 a        10491 c         8786 g        15853 t
ORIGIN      
        1 ggctaaaaga gaatcgttaa taaatgataa agaaattaaa aatttcttca aagtttaatg
       61 aaataattga tatataaaaa tagtgaaata acgcacatgt aagcttttac atataataca
      121 accttgttat ctgttatatt ttatagccaa atagcgtgac atggggcttg agaaatgtta
      181 gcataagcat gggaaaaaac aaatcaatgg aaggattgat actaaatgac tgcagctgca
      241 gaaaaaaaag atcatcaaaa agttattctt gttggagacg gcgccgtagg ttctagctat
      301 gcctttgctc tagttactca aaatatcgct caagaagtag gtattgtgga tatcaatact
      361 gctaaaactg aaggagacgc aattgacttg tctcacgctt tggcatttac ttcacctaaa
      421 aaaatctatt ctgctactta cgcagacgca catgatgctg acttagttgt tattacagct
      481 ggcgcaccac aaaaaccagg tgaaactcgt ttagacttag taaacaaaaa cctaaaaatc
      541 aaccgcgatg ttgtcacaca aatcgttgaa tcaggattta acggtatctt tttagttgcc
      601 gctaacccag tagatatttt gacttactca acttggaaat tctcaggttt ccctaaagaa
      661 cgcgtaatcg gttcaggtac ctctcttgac tcagctcgtt tccgtcaagc acttgcggaa
      721 ttagttgacg ttgatgcacg taacgtccat gcttacatct taggtgaaca cggagattca
      781 gaattcccag tttggtcaca tgcaaatgtt gccggcttgc aaatctatga atgggtaaaa
      841 aacaacccag aaatcgatga agaagctatg gttaacctat tctttggcgt acgtgacgct
      901 gcttacacaa ttatcgagaa aaaaggcgct actttctatg gtatcgcagt agcattagca
      961 cgtatcactc gtgccatctt agatgatgaa aatgctgttc ttcctttatc agtattcatg
     1021 aatggcgaat atggattgaa cgacatctac attggtgcac ctgctgttat caaccgtcaa
     1081 ggtatccaaa aagtaatcga aattccttta agtgacagcg agcaagaccg tatggctgct
     1141 tctgctaagc aattaaaaga aattctagat gaagcttttg ctaaactaga cgctgaataa
     1201 tctttgaaca tgacggaaaa catctattcg gtgttttccg tttttttatt ttatcaccct
     1261 tatggtttct aaattttttc ataaagtaga gataaccttc gtcttttcct ctaaattatg
     1321 ctatcatgag accgttccac cctaaacttt tacttaagga gttgtcgata tgacaagatc
     1381 ttcgcatcga aaaaaatcga aacatgttgc tttgctttca gttcttggtg ttctcttagt
     1441 gctgatcagc ttttatacct atcgcagtac atactacaca aagcattttt tgccgaatac
     1501 agaaataaat ggaataaatg tcagcaatct aacagtcgaa aaagcaaatg aaaaattaaa
     1561 agaagcttat tctgataaaa aactggctat taaagaagat ggaaaagtct ggcaggaagt
     1621 accaaaatcc gagctcggct acaaagatga tttcacttcg gagctttctc cgatcctaaa
     1681 tgaacaaaac ggatggactt ggggaatgac ttatgtctca gcggcagaaa aacaagaaat
     1741 agatcctgtc agtcaaaacc accaaaaact agacactacc gttcagacat tgacaactaa
     1801 gctgactgat ttgaacaaag accggacacc tactactgat gcaacgatcg aaaaagcagg
     1861 tgactctttc gttatcaagc ctgaagtcaa tggaaatacg atcgacgtcg atgctgcggt
     1921 caaacaatta aaaagtgcgg taaactctgg taaagataca attgaattga ctgaattcaa
     1981 agaaaaacca aaagtaacat ctgaagacag ctcactgaaa gaacaactcg catccatgaa
     2041 tgctatcgca aatgtaaaag ctacttacag tataaacggt gagactttcc agatcccatc
     2101 aagtgatatt atgagttggc tgacctacaa tgatggaaaa gtcgatttag atacagaaca
     2161 agtacgtcaa tatgtaacag atttaggcac aaagtataac acaagtacga atgacacaaa
     2221 atttaaaagt acaaaaagag gagaagttac ggtcccagtt ggcacttaca gttggacgat
     2281 ccaaacagac agtgagacag aagcattgaa aaaagcgatt ctggcaggcc aagattttac
     2341 tcgttcaccc attgtgcaag gtggcacaac cgctgatcat ccattgatcg aagatacgta
     2401 tatcgaagtt gatctggaga accagcatat gtggtattac aaagacggaa aagtggccct
     2461 tgaaacagat atcgtttccg gaaagccaac tacacctaca cctgctggag tcttctatgt
     2521 ttggaataaa gaagaagatg caactttaaa aggaacaaat gacgatggaa caccttacga
     2581 gagtccggta aactactgga tgccgattga ctggacaggt gttggtatcc acgattcaga
     2641 ctggcaacct gaatacggtg gagacttatg gaaaacccgc ggttcacacg gctgcatcaa
     2701 caccccacca agtgtaatga aggaattatt cgggatggtc gaaaaaggaa cgcctgtatt
     2761 ggtcttctaa caaaaataat atataaaaag aaagcacccg tacgattggt acaggtgctt
     2821 tctttttatt atctaacaat gtgctactta catttcagca gcagtaaaga cttctgaaac
     2881 atcatcatcg tcttctagct tatctactag acgttccaat tgctcttttt gttcctcatt
     2941 taaatcaatc gtcgtttgtg gaatcatcgt caattctgct tgcgccaaag tgaactcttt
     3001 ttccaactca tctcgtacag cagcaaaatc ttctggtgca gtataaatct cgaatacttc
     3061 aggagaagtt tgcagatctt ctgcccccgc ctctaataca tcctcaaaca tcgtctcttc
     3121 atcaacagtc aacccatcgc gttcaatagc gatatatcct ttgcggtcaa acaagtaact
     3181 gactgaaccc gtttctccca gtgatccacc gtttcgagta aaagctactc ggacatttgt
     3241 cgctgtccga ttacggttat ctgtcaatgc atggactaag attcctactc cacccggacc
     3301 atatccttca tacgtgattt catcgtaatt cgtttcatct acggcactcg tagccttttt
     3361 aatcgcccgt tccacattgt cgttaggcat atttgcagct tttgctttgt ccacaacaag
     3421 ccgcaaagct ggattcatag atggatcagg accgcctgct ttagctgcca tataaatttc
     3481 acgagaaagt ttctgaaaga ctttccctcg ttttgcatct tgggcatttt ttcttccttg
     3541 gatattatgc catttactat gtcctgacat gatttttcct cccttgttct tttgagaata
     3601 cacttctatt ctagtactat ttcacaaaaa aacaaagcca cagccaatct ataaacgctt
     3661 cttgaatgcc gcaaaaagca cacataatat cacagcagtc agagcaccga tcgaatccaa
     3721 catgacatct tggaacatag gtgttcgtcc accggtcatc atctgatgga attcgtccaa
     3781 acctgcgtac cctgttgctg aaagccaagc aaggatcgct gtcagccacc aaatttttag
     3841 cttggggaaa acacctaaaa acaagctgcc tcccaaaata aaataggtac cgaaatgtgc
     3901 gcccttcctt aaaaagaact ctaaaaattt ggcgtagccc agatggttga tactcaccat
     3961 acttccacca taattaaaac gtatacccgc taatgtgtct ttaaatggct gattaggcag
     4021 ccagctttct aatcgcgaaa cttgtgattg ctgtccgtaa gtttgagaag aactgtaaaa
     4081 taagattata acgactatca gtgcgattac taaataaata tttccgtttt tcatttgttt
     4141 ttttgaagaa ttcataagtc acctctgtat tttatcttag actttttttg aagaaacaac
     4201 aatcattttc atagagactt cttccataaa aaagtgctat acttaatttt gtattacaat
     4261 atgaagggag ttttttcatg agttattcat taaattggaa tctagattct attttttccg
     4321 gaggcagtca ttctgatgct ttgaaccaac ggatgaagca attggaagac caaatgaacg
     4381 agtattatca ccgcgtaaca aaatggtcac ctagttctga caatgcagag cagttaaacg
     4441 ctattcttca attgcaagaa acgattacga atggttttac ccaatgcagc agctatatta
     4501 ctgcattgct ttctgccaat gtcaatgatt cagatgcaaa ggtcttatca ggcaagcttt
     4561 acgcaatgct gcctcgtctg caatcagccg aaactgtatt gtcaaagaaa tttgcagaaa
     4621 tatctgacaa tgattggaat cagatgcttt ctcaatcttc gtttgaaaca attgcttttc
     4681 gtttaaacga gattcgtcgt gatggctcac aattattatc agaagctgaa gaaaacatta
     4741 taaatacgct ttctttagat ggtctaaatg cttggagcag tcattatgat acgattgtag
     4801 ccagcatttc gattcccttc gaacaagatg gacaagtagt cgaattatct gctggacaag
     4861 ccttcaataa gatgatgggc gatccagatc caaaagtcag agaaacactt ttcgcagcat
     4921 gggaagaagc ctggaaagaa aaggcacccc tcttcacgga tactttgaat catttagatg
     4981 gttttagact ttctgactat gaacttcatg gtgtcacaga ttttctacaa aaaccattag
     5041 agtacaatcg gttgaagaaa gaaacattgt ctgtcatgtg ggatacaatc cagaaaaaca
     5101 aacaacccct tgttaattac ttgactagaa aagctaatct tttcggtaaa gaaaaaatgg
     5161 aatggcaaga tcaagatgca cctattattt taggcgattt gaaagaaaaa acctttagtt
     5221 ttgacgaagc agcagcattt attatcgaaa actttaacaa attcagtcca aaaatggcta
     5281 aatttgccca atctgctttt gaaaagagct ggatcgaagc agaagatcgt cctgggaaac
     5341 gtcctggtgg ttattgtact gagttaccag aaacaaaaga atcaaggatc tttatgacct
     5401 acagtaattc tgttaatgag gttgcgacac ttgctcatga actaggacat gctttccata
     5461 gcagcacgat gtgggattta ccttctttaa atcgcgaata tgccatgaat gtcgcagaaa
     5521 cagcaagtac attcgcagaa ctgatcgtag ccgatgctac attaaaagtc gcaaaaacaa
     5581 aagaagaaaa aatcaatttg ctagatacaa aactgcaaaa tgctttagct atgttcatga
     5641 atatccattc ccgattcatt tttgaaaatc gattctatga agcacggcaa gaaggattag
     5701 tctcagaaga taagatcaca gagatgatgg ttgaggcgca aaaagaaggc tatcaaggag
     5761 ctttagctac ttaccatcca tatttctggg ctgcaaaatt gcatttcttt attgatgacg
     5821 taccgtttta taacttccca tacacatttg gctacctctt tagtttgggc atctatgctt
     5881 acgccaacca aaagggagca aattttgaag aagaatatat ccatttacta cgcgatactg
     5941 cctcaatgac gacggaagat cttgcgaaga aacatttgaa tgtcgatctt acccaaccag
     6001 atttttggca agcaggtatc gacatggtga ctgaagatat ccaaacgttc atggaattga
     6061 cagaagaatt tatctgattt ctattttatc acaataaaaa aagagttagt ccagaatggg
     6121 ctaactcttt ttgattaatc attcttacat gctgacagca aagtcagggg caaaccattg
     6181 aactgaacca acttttacgc tttcgcctgg ttgaggagca tggatatatt gtcctccgcc
     6241 taaagcaatc gctacgtggt aagttccgcc ttgtgacccc cagaataaca agtctccagc
     6301 ttttgcttga gaaactgaaa tttttgttcc agctgattct tgaggaactg tccaaccacc
     6361 gatgtcacga ccagttactt gcatgtaaac atagcgtgtg aaccctgagc agtcaaagcc
     6421 acttggatct ttaccgcccc aaacatatgg agtaccaata tatttgtaag cttctgctac
     6481 gattgctgca ccattcacac ttccagaagg agctggagca ggtgttacag gttttggttg
     6541 ttcaggtgtt ggtgttggcg ttggagccgg agtactactt cctgtgccgt ttcctgtatc
     6601 aacactttga tctgttgatg gtgttgtagg cgcaggtgtt gttgattctt ctgttgttga
     6661 agattcaggc actgttgttg attcttctgt tgctgaagat tcaggcactg ttgttgattc
     6721 ttctgttgct gaagattcag gcgctgttgt tgattcttct gttgctgaag attcaggcgc
     6781 tgttgattct tctgttgaag attcaggtgt tgttgattct tctgttgctg aagattgtgt
     6841 agctgatgat gattcttcag tcattgaaga ttgtgtagct gatgattctt ctgttgctga
     6901 gctttgtgca gtagatgaag cttgagtagc ttgtgcttca gcctctgctt gttcgcgtgc
     6961 ttctttttca gctttttctt gtgcagcttg ttgacgagct tgttctgcta gacgttgttg
     7021 ttcacgaata cgagcttgct ctgcttcagc ttctgctttt tgacggttca agtcagcttt
     7081 cttgtcttca gcagttgctt gttcagcagc taatgaagtt ttcagtacgt tcaaatcagc
     7141 ttgtttagat aaaagatcgc ctttttgaga ttccaaagca gcttggtttt cagctaattc
     7201 ttttagttta gcatcgtttt cagctttttt gtcttctaca gcttttttat cttgtttttg
     7261 ttgttccatc aagtcgttgt ttgcttttac catcgttgtc attgcttgaa cgcgtccaat
     7321 agcatctgct aatgaatcag cattcaatac agcatcgatg tagtttgagc ttgtgttgct
     7381 tacttgtgct tcgcgtgctt gtttttggat tgtatcttca cgtttttcaa tacgttcttg
     7441 taaatctgca atgtctttta ctaattgtgc agattcttga cgcaatgtat cttgttttgc
     7501 taataggtct tgtgcttgtg tgttgatttc agaaacttgg ctttctaagg catcgatttg
     7561 tgattgtgca tctgcttgtt gatttttcaa gtctgcaatc ttttgatctt gctgttgtat
     7621 ctgagaatca aaatcgtccg ccgcggcagc gatcggtgat gctacggcag tcaaggtcat
     7681 tgaacatacc attactgctg atattaaact ctttttcact cttcattcct ccgactggct
     7741 taatttaata aatatctaaa tatatttaag tctttcttaa cgatataaaa attgtaccat
     7801 agcagtgtgt caacgatata atagttatgt tacaaaaatg tttcaaaaca acttttaaaa
     7861 atgacaaaac tgttcgtttc agaaaatgat catgaaaaag ccgcgttaag tctagcattt
     7921 cttaagataa caacgattct ttttcgcttt cgacctaaaa ataaaaaaag aaattattca
     7981 tcactgaata atttcttaaa gggaaaaatg aataatacaa atataatcat gttcaacagc
     8041 agtgtaggtc ctaaaaacct tgtaataaaa tacgtgttat ttactactgc taatttaaaa
     8101 atcaactgga ccaccatcgt aaacaattca taacccgtga caaagatgat catactgaaa
     8161 aattcagtaa agatgttgac atggataacg tctttcatta catacatcaa ccatacaatt
     8221 aaagggagag ctactgcata gataccgata acgccaatat agtaagcgtc atagatagcc
     8281 ccgagaacaa tcgtggtaat cagtaaataa cgtttttcaa acgccatact gcaacataat
     8341 aatgccaata tcagaaagtg ggcatttgac atataggtgc ctttcgacca ctcccctagc
     8401 attcttgtta aatgaccatc tatcaacatc aaaaagaaca aaacgattgg cagatagtac
     8461 ttcattgttt ctttcttcaa catggactaa tcctccgcta gccgttggac aatcgtcaca
     8521 actgacacgt catacatttc tgcataaggt ttcacataaa cttcccgatc cagcccataa
     8581 ctatctggtt tcgttttaat aaccgtaccg atcggcaagt tagctggaga attccctcca
     8641 agaccggatg tctggacaac atccccttct ttgatatccg tatcaccagt taattgggtc
     8701 accactaagg catggagctt ttcatcatag tttttaagca aaccaaacgc ttcgccatta
     8761 gccgaagata cccgtactgg aaaatgattg gagctttcat tagaggatga cagtaattcg
     8821 attttagacg aagccgtatt gacctcgatc actcggccaa tcaatccttt ttgcgacatg
     8881 actgccatat ttgcttcgat accgtcttta gaacccttgt ctacgatcag catgtcctgc
     8941 caactgtcag gcgaacgagt aatgacattt gccgttactt tttcgtaact agtcagcgtc
     9001 tgattcaact caagttcttt tttcaacgct tcgatttctc tctcgtaatt cttgttttgc
     9061 tgtgaaacgt catcatagct atcgattttt tcttttagcc gctcattttc ggcataagtt
     9121 gaaaaaagat tatggacaga atctactcca tttcgtaccc accgtactgg ggcagagatt
     9181 gttttatcaa ccattgcaac actgtcatta acgatggatt ggaagaaatt gggctttcct
     9241 tcattcgctc gctgcgcagc ggtcacactt agtatcgtca ccacgatgat aacaataacc
     9301 aaggtaataa taatattttt attaggattg aacttcttca caaagacacc tcgaattaat
     9361 tagactcttg tgttcaattt tatcactaaa tgccgtgaaa caaaaggtaa gttgctcctc
     9421 ttaagctttt ttttagcaaa taatagattc tgtcattttt agcctgaaag acaaattttt
     9481 ttatttgttt atggatcaat ctttgctttg aataactgca atcataccat ctgaaaaaga
     9541 aaattctgcg gtatctgaaa gaaattcgtt acttgcataa gatgtcggat attctacttc
     9601 tttattttcc aatacatact tgctgccttt taatgtaaga ttcgagacgg gagtcaagca
     9661 gcaataagct aaatacttca tatctggtat ctgtcttact atatgctctc caggcaaata
     9721 ataagacaaa taattttgac aatctctgat aataagctga tgggcaaagg gtttgaagcg
     9781 aggttccata cctaaccaga gattggctaa aagatgatcc aatcttccgc ccgtagctcc
     9841 aatcaaagtg acttccgctt gcgggtaacg ttcaaatgtt tttaagatac ctaactgtgt
     9901 atctgtatcg tctttttctg caggggaacg ggtatactct ttggcttttg caaatacttc
     9961 cttctgctct tcgttcgaca atgaatcaaa atcacccact gccagatcta ctgggtagcc
    10021 tgcttgaagc aggtacagcg caccacggtc tatcccgaca taggcatcgt atttggacag
    10081 cagagactgc ggccagtttt gcggattacc tccagcagct aataaaattt tcatttagtc
    10141 taatgcctct tttaatcgtt cgatttgtcc gactggattt tcggctccat agatatatga
    10201 tcctgcaacg aagacatccg cccctgcatc tttacagatt tttgctgttt ctggaacgat
    10261 tccgccatct acttcgattg cataagtcca attgttctct ctacgtagat cagcgagttc
    10321 cgtaattttt tctactgttt cttcaataaa gctttgtccg ccgaacccag gattgacagt
    10381 catgactagt acttgatcag ccaaaggcaa aacatgtttg atcatggaga ctggtgttcc
    10441 tggattgata actactccag ctttaactcc taagcttttt gtcatttgaa gtgcccgatg
    10501 aatatgagga gttgcttcga cgtggacagt aatgatatct gctccagctt ttgcaaatgc
    10561 tggaatgtag ttttctggat caacgatcat cagatggacg tctaacggta atttagtaac
    10621 aggacggatt gcctgaacaa tatttggccc taatgtgata tttgggacaa atcggccatc
    10681 cattacatct acgtggatgt aatctgcgcc tcctttttcc actaactcaa tgtctctttg
    10741 taaattggca aaatctgcac tcaaaatgga tggtgctatc ttcattcttg tttcctccta
    10801 ttttcgttta ccttcttttt ttgcatacat tggttttctt ttttcgattt cttgtaagaa
    10861 ttgcaaataa ttgtcgtaac gtgtctgtgc gataccacct gtttctactc tatgttttac
    10921 ttcgcagcca ggttctttag catgcatgca ctctctaaat ttgcaatgga cagaagcttc
    10981 tacaaattca ggaaattgtc tcggcagttc tcctgcttca attgtcaaaa agtcaatcga
    11041 gctaaatcct ggtgtatctg ctactaaccc gccatagata ggaatcagtt ccacatgacg
    11101 tgttgtatgc cgtcctcttc caagtgcatc agaaatctct cctgttgcta aattcagttc
    11161 cggtgatatt tgattcagta gcgtcgattt tcctgctcca gactgaccca taaagaccga
    11221 tagccgttct gggaagtaac ggatcagttc tgtcgtatca cccgtatgat tgggtacaat
    11281 caccggatag cctattttag tataggtttg cttgatctct tcgatttttt ctgggtcgtc
    11341 tgacaagtcg gtctttgtca aaaaaataat cggttcgatc gactcgtatt ccaaggtaac
    11401 tagaaagcgg tcaagtagat tataagaaaa tgcaggctct acgatacttg tgacaatgat
    11461 tccaagatca acatttgtga ctggtgggcg aactaattga ttttttctcg gcaagatttc
    11521 cagaagatag ccgtctgttt gattttcact ctcaaagatc acctgatctc cgacaagcgg
    11581 cgtgattttc cgattccgga aatttcctct tcctcttgtt tgataggtct ctccgtttgc
    11641 gtatacatag taaaaaccgc tgattgcttt tctgatttgt cctttgataa gtgccacctc
    11701 cgtttttgtt tattttacca tacgaagagg aaagtaggtg ttattccatg caaacttctc
    11761 aaagtatatg gcttgaaaat tgatcaaaag agatagaaac acaaaaaaga gaatgcaaca
    11821 aatcaatgtc gcattccctt ctgatatatt ttattcagca cttattttga actttctgag
    11881 gtgctagctg atgaacttgt ggagctggaa gattcttctt ttgaagagtt gcttgtgcta
    11941 ctggagctac ttgagctgct agaactgtct gaagagtctt tttcagaact agaagacgct
    12001 ggatctggtc ctttagagta aattacgttc acaaccattc ctttggtaat tgtcgttcct
    12061 gcccctggta ctgtacgaat cactttatct ttatccaccg tgtttgaata ttcggaagtc
    12121 tcatgcccga tatattctgc accgacacta gctaaataac tttgtgcttc gctttttgta
    12181 tagccggaga tatcattcaa tttaggctct gttccttggc ttacgacaaa attgatgtaa
    12241 gtttcttctg ctacaacttc acccgaggct ggatcctgac tgatgatcga acctgatggt
    12301 acagtgtcgc tatagtcata agtagcacta atattattga tattgaatcc ttgtgtaaca
    12361 agccaattac gaacagtttc gtagttttct cctacatgac tactcaaagt cactgtttct
    12421 ggtcctttgc tgactgtgaa agtaatcgtg tctgttttag gatcgacttc tgtatcttct
    12481 gatggagatt gcgaaatgat cattccgctt tctacctctg aactgaattc ctcttctttt
    12541 tgaatcaagt cagcagaaaa ccctaattct ttcaaacttg cttcggcatc ttcataactt
    12601 ttaccactgt aatcatctaa tttgattttt ttcgtgccgc tactaatata cagagtgact
    12661 tcgcgttttt gcttgactgt cgttcccgct gccgggtctg tcttcactac attaccttct
    12721 tcgattttgt catctgcaat ttcttctgtt tttgttgcta cttctaattt cgcttttgcg
    12781 agtgtttctc tagcagaagc ttcagaaaga tcagaaacat caggaacaac aacttctcct
    12841 cgcccgccag aagcaaagaa tacagctgtt ccgattccta aaacagcaag aatagctaat
    12901 aggatcagcc accatttttt ctgtcctttc tttttaggat caggcagaac ttcttcctcc
    12961 tctggtaagg tttcgttttc tggcttgtct tttggcagag gcatagattt gaatgaatca
    13021 ggcattggtg catcttccgt aaggggtgtg ataacttttg tttcattatc catcacatga
    13081 ggctgccatt tttcttcgtt tgcacgagct ggtgataata ctgtagaaag atcatcagac
    13141 atttcctcgg ctgacttata acggtctgca ggttctttag ccgttgcccg taaaatcacg
    13201 ttttccagtg cttgtggcac atttggatca agtgctttta tagatggaag atcatcttga
    13261 aaatgtttta aagcaattgt tacagcagat tcaccatcaa acggtacgct acccgtcaac
    13321 atctcataaa ggatgatccc taaagcataa atatctgact gcttcgttgc catacttcct
    13381 ctggcctgct caggggacag ataatgaaca gatcctagca tcgtattcgt ctgcgtgatc
    13441 gatgtttcag ataaagcgat agcaatcccg aaatccgtga tttttacaac accttcatta
    13501 tcgatcagta cgttttgggg tttcaaatct cggtggatga tttgatggtt atgagctaaa
    13561 gaaatagcag aaaggatctg ctgcataata tttactgctg tttcataagg aacaggataa
    13621 tgtgtttgga tatatcgttt caagtccatt cctttgacgt attccatcac cagatactgc
    13681 atgttgtctt cttcaccaac atcgtaaaca ctaacaatgt ttggatgaac gagttctgtt
    13741 gctgctaaag cttctctctg aaatcgtcga atcgcagtct ggtcattttg aaagtcaaat
    13801 cgtaagactt taactgctac atcacgatcc aagattaaat cgtgcgccaa aaatacattt
    13861 gccataccac cgctaccgat atttccagtg atttgataac ggccatttaa ttttttgccc
    13921 aactcgatca tacactgtcc tccttatatt cgataagaag aacggtgata ttatccgctc
    13981 cgcctgcttc attggcacga tcaatcagct cagttacttt atgagacaat gggccttctt
    14041 gattgatgat cgttcctatt tcttcttcag acagcatgtt cgtcaagcca tctgaacata
    14101 acagcagtcg atcttttagt tgccaaagat gagtagaaac atctacttcg actgttccag
    14161 gcatgccgac agaacgtgtt aaaatatttt tccttggatg gttcactgcc atttcttcgc
    14221 tgatttctcc tgatttgact agttcattga ctaaagaatg atcctccgtc aattgcatga
    14281 ttttatcttc acgaatcaaa taagctctgc tatccccaac attggcaatc gtgaaccagt
    14341 catctgacag agcggccgct acgattgttg ttcccatacc gttatattcc ggctgttctt
    14401 gtccacgctg ataaatcagc atattttctt cttgaatcga ttgaatgaac cattgggcga
    14461 ttttttcatc gtcaacagct tcttgttttt cccaagcttc gcctagattc gttaccgcca
    14521 tctgactggc aatgtctccg gcacgatgac cacccatccc gtctgctaat acagcaagct
    14581 tgagacctga acggctggta aatacactgg catagtcttg atttgtgtta cgtcgtcttc
    14641 ctacatctga ttgatattca atctgcattt tttcacctca ttactttttg cgcaaacaac
    14701 aaatgaaaaa tccatctgtg taaaactgat gcggatacaa agtcaacatt ttctcttcga
    14761 ttgatgattg tagcatagga ttggtcaaaa catcaatcct ctcaaaatca gggtgtcttt
    14821 ccaagaaagc agaaacaacg tcttggtttt cttcttttaa gatcgtacaa gtgctataag
    14881 tcagaatacc ggaagacttt aaagttggtg cgcagctttc taaaatttcc aattggattt
    14941 ttggtaaatt cgctaattca tccggctgtt ttttataacg aatatctggt tttcgccgaa
    15001 gcagcccaag acctgaacaa ggcgcgtcta ctaaaatccg atcaaactgc tctgcttcga
    15061 actgtttact gacttctcta gcatccatct tttgagcgta gacagcagaa gagacaccta
    15121 gacgtgctgc attatcttca atgagtttga ctttgtgttc gtgtacatct aaagcgacca
    15181 cttctccacc gcatgacgga tctaaaaaag aagcaatatg cgtcgtctta cctccgggtg
    15241 ctgcacaggc atctaagaca tgatgttcag gctcgatttg catagacggc gctacaagca
    15301 tcgaactttc atcttggatc gtcatgacgc catcgtggaa caatgagcta gaagctaaat
    15361 gccctttttt cgctactata ccgtatggac tgacttggct tttttcagct gtgatctctt
    15421 cttcttgcaa aacagtgatg gcctgatctc tggaaagtcg gcgtgtatct actcgggcac
    15481 tagcatggct aggctgatat agcgataaac cgagcttttc agtttcttcg tatccgattt
    15541 gatccaccag tcgtttcgtc atccatagag gcaggctgat tttgacagaa agtctttcga
    15601 taggatcagc aatgccttgc agatctggtg ctccttgacg ctgataattc ctcaagacac
    15661 catttacaaa tttccctgtc ccaggatttc cttttacttt tgcgatttcg accgcctcgt
    15721 ttaaaatagc atgtgcaggg actttatcta aaaattctaa ttggtataca gaaagataaa
    15781 gaagcatctt gacccagtca tccacttttt ttgctttagc gatcaatggt gcaacgtaaa
    15841 attccaatag tcgctgatgg ctaatcgtac catagatcaa ttctgtaaat aaacccacat
    15901 ctttttgatt tagctcgctt tgtttgatca tctcattcaa caaaagattc gagtaggcgc
    15961 cacccttgtt cactcgctct aatgtcagta atgccatata tcgtactgat tttttcattt
    16021 ttttagggat ttttttcatt atttcacctg ctgaccaatc tgaacttgct gaccgcttcc
    16081 gttaaggaaa tcatgtacgg cctgttttcc tttaccagct ggctgtaatt cattgatggc
    16141 taagacggtc tgtttaccgc aagcaatcca tagatttttt ttgtctttcc ggatgatcgt
    16201 accaggtgct ttgtctgtag tttctgccaa tacttcaacc gataataatt tccagcgtat
    16261 gccctcatag gtcgtgaatg caattggcca aggacgcatt ccgcgtactt ggttgtcaat
    16321 ttcttctgca gttttatgcc aatcgattgc ttcttgttcc cgtgtgatgt ttggcgaaaa
    16381 agttgcttct gactcgtctt gaggtactgg tttttgtcca tccaaaatat tgggcaatgt
    16441 ttctagcaat agctttcttc ctaaaaggct aagcttttca aacatcgtcc ctacatcatc
    16501 ttgtttagtg attggtatgc tttcttgcgc atagattccg cctgcatcca tttttttgat
    16561 catttccatg atcgttacac cagtttcttt ctccccattc atgatggcat aatgaacagg
    16621 cgcacctccc cgatatttag gaagtaaaga agcatggaca ttgattgccc ctaattttgg
    16681 tacttgcaat aacttttcag gcaagaattg gccaaatgcc gcagtgacga tcacatcagg
    16741 caccaattca atgatttctt ccatttctgg cgaaccggag attttctctg gctgcagcac
    16801 acggataccg tgcttcagag ccgctttttt taccggtgtc ggcgtaatta ctcgtttccg
    16861 cccaactgga cggtcgggct gtgttaccac tgcctggatc tcgtaacctg attctatcag
    16921 cccttcaaga atcggcacag aaaaagcagg tgtgcccata aatactagct tagtcatcaa
    16981 gatgttcctc catatattct tcaagttctt cttccgggat ctgctgtatc attttctcaa
    17041 tgaacaagat accgtctaag tgatcgattt catgctggaa ggcacgggct aaatagccaa
    17101 aagccgtcac ttctatctct tctccgtctc gatcataata acgcacggtc acttcatctg
    17161 ctcgcttgac cgttccatag acatgcggaa tactgagaca accttctacg tcaaggcttt
    17221 caccttttgc ttcgatgatt tctgggttaa tcagttcaaa tttctcgcct tcatctactt
    17281 cgatcacagc aattctcttg ttttgtccta cttgaggagc agcgatccct attccgtcat
    17341 tcgcaatcat tgtctcatat aaattgtcta ataaagcgat tgtctcgtcc gtgattacat
    17401 cgattggctg agcagttctt tttaatttat cattagggtg gatcaatata ggatagcgca
    17461 taatttggcc ctttcttaaa taaagtttaa tggttcgttg tcaattgcca caaaaagacc
    17521 ttgtcgctgt tctttctggc ttccattcaa aatttcatcc aaaacatgag gaagattcgg
    17581 ttcatgttta tatttgatga ttagttgata ataatatcgg tttttcactc tcaaaatcat
    17641 gctgggcgtt ggccctaata gcaagctttg agggcttaac acttctttta gctgattggc
    17701 aatctgaaag attttctttg ccgctacttg ttcttcagga tggctggcgg ttattttcac
    17761 agtaaagtaa taaggaggat agcctccacg atgccgtaca tgcatttcct gctgataaaa
    17821 ctgctcataa ttttgttttt tagccaattc tatggcgtaa tgttgaggat tgaatgtttg
    17881 gatcacgact tcgcctgctt tttccgctcg ccctgctcgt cctgaaactt gggtaagcaa
    17941 ttggaatgta tgctcactag aacgaaaatc cggtaaattc agggaggtat cagcattcaa
    18001 caccccaact aatgtcactt ctggaaagtc caaaccttta gcaatcattt gtgtccctaa
    18061 taatatatct gcttcttttg caccgaattt ttgtaaaatc tgttcgtgtg ccccttttct
    18121 tcgagtggta tctacatcca tacgcaaaat cctagcttgc ggataaagtt ctcggagttc
    18181 ctcttctact ttttgagtac ctgttccata gtagcggatt ttattgccgc cacaattagg
    18241 acagcgattg ggaataggtt cttcatgtcc gcaataatgg cagcgcattg aacgtgtatc
    18301 catatgcagt gtaagagaga tatcgcaatt aggacatgga agaacaaatc cacagtccct
    18361 acacatcaca aaagacgaat atcctcgacg attgagcagt aggacagttt gttctttttt
    18421 gtctaatcga ttttgtattt tttcctgcaa attagcagaa aaagtagagg tccgatgatt
    18481 ctgaaactct tcacgcatat ctacgatctc aatagctgga agctgtgcag ctgctttggc
    18541 tcggtggttt aactgcaata actgataaac gcctttttga gctcgtgctc ttgattctaa
    18601 agagggcgtc gcgcttccta gaactattgg gcaatgatga tatttactgc gccaaattgc
    18661 tagatctctc gcatgataac gcggtgtctc atcttgtttg taacttgatt catgttcttc
    18721 atcgataatg atcagcccga tatttttcag cggggcaaaa atagcagatc gtgcgcctac
    18781 tactacttgc gcttcttccc gctcaatctt gcgccattca tcatattttt caccttggga
    18841 aagtccgcta tgtaagaccg caactgattc tccgaagcgt cctttgaatc gctcgaccat
    18901 ttgcggagtc aatgcgattt caggcactaa cataatagcc gttttatttt ctgacaaaac
    18961 atcagcgatt gcctgaagat atacttctgt tttcccgctg ccagtgattc cctccagcaa
    19021 aaagacctga tctttttgct gctgacctgc tgtgatgatt tgttcgacag catttttttg
    19081 ttctgcattc agttccaatg ccgtcgtttg gtcaaatact cggtctttat aagggtcgcg
    19141 ataacgttct gattctataa actccagcca gcccttttct tttccttgat tcaagacagc
    19201 agtactgatt cctttatttt tgaaataagc aattggttct tcttggctca gttcagataa
    19261 acacaaaaga agttcttttt gtttcttggc attttgccgt aattttgccc attcttcttc
    19321 gatttgcttt tcagttaaca aagaacggac caaacgaatc gtttttactt tatttcgtgt
    19381 atggacttca tatttgatat ctactaattg ctcttggcgc cagcgaatca attgagaaag
    19441 gctgccttct tcttctgctt ccttccagga aatcacttct cgctgatcaa acaatacctg
    19501 aacttctggt gtgtctgcca aaggatagat caatttatca tattctgctt tcatcacact
    19561 cggaagcatc gtttgtagac aagtgatttt gaatgcgtaa gtaatttttt tcatataatc
    19621 agctaatgaa agtaattctt cattgaccac cggagcaagg tctagcaaac gaataactgc
    19681 cttcaaagat ggattagctg attcttctga tttttggata gccataacaa atccttggac
    19741 atgacgattg ccattaccaa aagggacttc tactcgcatc ccgacttgca aagctgtttc
    19801 catttcagag ggaaccaaat aagtgaatgg ctgatcggtc tgcatagtag gtacgtccac
    19861 aatgacttca gctgttaatg tcatggttcg tttcctcctt tttttgtctt ttcttatcaa
    19921 gcgacaaaaa gttccttgcc tattttacta gagatcgatg gatttgtctt atcttctgcc
    19981 aaatcctaac aaaaaattaa cagcagataa gaaaaaaaag aactgtgtca aaaagttcag
    20041 acacagcctt ttctaccaga caataagtgg cgaatgtttg aaaaggaaac agctgggttt
    20101 gcttttcttt ttagatgttc gctctatttt tctttattac attttttgct cttcacggat
    20161 acgtgattcc aattcgtgtt gttcacgctc tttttgtgct tgacgttcct cttcttccat
    20221 cttcaaacgt tcacgtttta attcaggatg tggatcattg actacatttc ctgcatcgat
    20281 ttcttctaaa gcttgaccga cgctttttac tgaatcaaaa gaatctagag taggctgtgc
    20341 acttgcatcc aattcatgcg cgcgtttact tgctaaaatc actagagaat attttgaatt
    20401 tacacgatct aatagtgagt caattgaagg ttttaacatc ataagactac atctccttta
    20461 acattttaat atatttaccg attacgcgat ctactcgaaa atgttcactg gcaataatgt
    20521 ttttgattcg ttcaactgct agcggcactt catcatttac aacagcatag tcgtaaagag
    20581 ccatcatttc gatttcttct tttgcaactt tcattcgttt ttcgattact tcatctgcat
    20641 ctgttcctcg gcctacgatc cgtgatttta attccgctaa atctggcgga gtcaaaaaga
    20701 tgaatacacc atcgggcact ttttctttga cttgttttgc tccttggact tcaatctcca
    20761 agaaaacatc ttttccttca tccaatgtct tgttgacata tgacaaagga gttccataat
    20821 aattcccgac atattctgcg tattccagca tctctccagc ttcgatcatt gattcaaact
    20881 cttctttgct tcggaagaaa taatcaacgc cttcgacctc cccttcacgc attttacggg
    20941 tagtcattga gatcgaatac tgaaaatcat tatcctcact ctcaaaaatt gcttttcgta
    21001 ctgtgccttt tcctacaccg gatggaccag acaacacgat taataatcca cgctctgaca
    21061 tgacgacgtc ctttcaacta gtctttttgt acttatcgtt tattttgcac tttttttctg
    21121 caactttcaa ggttatcttt aattatagct agaaattggt aaaaaagcta ccctgtacca
    21181 agaggtcgta aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca taaacagctt
    21241 ctcatgtttt tcgtttggtt ggggttatta tcgcagatgc aagcttttga acgccgtgta
    21301 taagaggtcg tgaagaagcc gattagtttc gagtatgaag aaaaattttg tgaaaatagt
    21361 ttctcacatt tttgcataaa tttggcttaa taccgagaaa atggtttttg aacatcactt
    21421 attcaaaata aaaaaagatt gtgacacgaa ttttgtcaca acctttaaat gtattcctag
    21481 agcaaagaag ctaagcgaag aaatccaatt ctgtttattt attgtatttc tttgttcagc
    21541 atccttgcta aaagcccata ttggacaaag tattcactac acccatgatc tcatcttttc
    21601 gagaacgtgc tttttctgtc ggatcttcta cctcttctgt gtcaattcga acaataaatt
    21661 tccgattcat cgtcacgtaa gcaccgtcgt tgatcaaaat aaacagttcg ttctcattat
    21721 agtctgcaca ggcatctttc catacgtcaa agccgttttt ttcagaaggg atatcccgtt
    21781 taaccacagt ttttccactt aattcgtgga acgttacgtc ataatatttc attatctcta
    21841 cctctttcgt cattcaatat tttgtacttg ttctctgatt ttttctaaga tcgtcttaga
    21901 ctggacgact tgatttttaa tttcgattgc atttgatttg gaaccgatcg tgttgatttc
    21961 ccgattcatt tcttggatca ggaaatctaa ttcccgacct acaggacatt gtgcttcgat
    22021 aagttcatct agtttttgta catggatcgc cagacgatcc agttcctcgt gaatatctcc
    22081 gcgttccaac aagatggcta gctccgtcaa caaccgttgc tcatcgactt tcgctccaag
    22141 aaattcctcc agcttttttt gataccttat ttgaaattcc ttttcataga tttctacaaa
    22201 actatcgagt ttttttagta cttctttgaa ttcttcagta ttctttttca acactaaagc
    22261 taaagcttga ccttcttttt ctcgactcgc ctcaatagaa tcaagtgcgc ttttcaatgt
    22321 atctagagct aactgtgcca attcttccat gctctcttcc ggtttttctt ctaccgttac
    22381 gaagttttcg tccagcacga agcgttccgc catccgacta attgacactt gattttgact
    22441 accgtatcgt tgttgaattc cgtctgtcaa ttgagtgata agtgaatcaa tcaattccca
    22501 gttgatgacg atctgttttt cgtttttgcc aatataagct agattcacaa atacttcgat
    22561 tcgaccgcgg cttaactgct gtttaataag ctgacgaaaa tcgttttcaa caaagttcag
    22621 gattttagga cttcgtattt gaacatctag aaaacgctgg ttgacacttt ttatttcaat
    22681 atctaactga tacgcttttg tttcgcggat ggctttgcca aaaccagtca tacttttcat
    22741 tcgacagggt ccttcctcat tacatatttt ctattgcttt ttgcagttgt tcaaactctt
    22801 cgttcgtcaa tgtcaggcct ttccccattt tttcatggtc tggcgcccaa tctcgaagat
    22861 cgaattttgg cgggcgacca ttccagctga ctaggttcag ttctttacgc caccccttgt
    22921 tgttttctga caatatagcg atttcttcta cgatttcata tgaaaattct tgtgccatgg
    22981 ttgttcctcc ttgagataat ataaatatag gatcagtaat gtgtcaactg gttgcttaag
    23041 caattcatgc tgcaaacgat ccttgcgttt tccttgtagc agataatcaa aataaacagt
    23101 tttcgtgcgc tcattttgta ttttttgctg gtattttcga taccttcgcc attcttcagt
    23161 acttaattga ccaatttgtg ccaataaatc atccggcata ctttttatat cttgttggca
    23221 gatcatttga atcatcatgt ctacttgata gatttcattg ggcagtaaaa aatgtttttt
    23281 tgctaaaagt tctagcatcg ccaatccgca tgtctctggt acgaccctgt tcttatataa
    23341 attacgcagt ttttgccaaa attttatgct tttgaaaaaa gataattcta actgaatttc
    23401 ttttgttaag taagacaaaa atcctcgcca gtatgttctt gcaggtttaa cagaatgaaa
    23461 tgtataccag ttcccatagt aatagcctaa tcgttttttc atttccagat agcctaattg
    23521 taaatttaat tggttccttt ggctatcaaa aagcagaaag ctgcctagtg tccacaatgt
    23581 ttgacatttc caatggataa aaatgtctgg catacgaatt tttttcgctg ggcctggccc
    23641 ttgcacatct acgacaaatg cctctgttgc tcctttgtta atcgcaatat caataggtat
    23701 tttgttgcga tacccaccgt ctgcatattt attttttccg atctttcgat aagccatagc
    23761 gggataaaat gaagcagagg cgagtatcca atctggtatt tcttctgttt ttgtctgttg
    23821 gatatcagtc actacttccg ctagttctgg cagtttagta gacaccgtat ataaacgtat
    23881 tttgggatta tgtttgagta aggacaaatc caaattgtct ttgaccatct tttccaatgg
    23941 agcagaagaa accccgcctt caattagtgc tgttttcgtc atttggcgtc tttctcgata
    24001 gaagcgttca cgaaggtctt caactgcagc catttctggt aacgcaagca cttgtcttgt
    24061 cgacagcttt ttccataaat tcacagcttt tttcatatct ccttgtaaaa tcaatgcgcc
    24121 gttcaacgca cctaccgagg tacctgtgat aatctcaaag ttgatatcat gttccttcag
    24181 cgcctgccac acaccaatct gataagcacc gtgcgctcct cctccaccta aaacaagtgc
    24241 tgtatgatag accagctttt tatttaattg tgtggttcct tgatagccgt ttttatgaaa
    24301 aatacgcaac cattcttctg atagaggaga ttgaaataaa aaacaacagt tttctttaat
    24361 aaaaaaacga gcgcaagctt ccatataccg aaaaaatttt ttccattcaa aagtttcacc
    24421 ttcaatcaac agattattaa aaataacatc tcctctactt ttagcaatag taaaatagac
    24481 ggtatatttt ttcgatactg ctttaaaagc acattgcttt tttgcagaac gccaaaaagc
    24541 gtctctactt tcttgatgac tttgaaaaaa tggaaggggt tgttttgctt gctggatagg
    24601 cgaatacagc gacattttct gtgaaaagta aacttttttt atctccatcg cgctctcctt
    24661 tttcttcaag tataccagta cgtcataaaa aggtcatgag tctcgtattc gactttgtag
    24721 attccataag aaaacaattc ataaaacctc ctttaaataa aaaaatacaa aaatcacaaa
    24781 aattaattga aaatgagagt gttttcaatg aaaacgtgat tttgtaatgg aattttaaaa
    24841 taaaagaaac aagctttttt aatgcaaatg ttgtaaaata aaagagatta agaggaaata
    24901 agaggaggaa catgatgact gagtcagttt atgtacatat tgataccaca agtaatgctg
    24961 tttcaacaaa agggctgact gctactgact ttgcaaacag tatcgtgcat tttcctcaaa
    25021 acttgctctt gcttgatcca tctgcttctg ctggagaata tgaatcccat acagggctga
    25081 aagtaatccg tggtacagaa aatattcagc gtttcttttc ctcttctcgt aacaggcgtg
    25141 gattcgatga attgaagtgg atcgacttta ctgatttaac gatgctgaaa gaattgacac
    25201 ctttagaaat ttcagaatta ttatattttg gtcatatgaa aacacatctt cattcgccgt
    25261 ttttttataa actacagaat aattttgtat acttcgatct taatgatcag ctgaatcgga
    25321 tttattatcg ctatttagat gaattttata ggatttttgc tagtaaatta agttcgatct
    25381 tgtcagataa gatcaatgta aaaaaatctt ttttcaaaaa agaaacacct gttgaaaaaa
    25441 tcagtgtgga attattgaaa gaaatgcgta atttgatgca ggaagggatc attttttctt
    25501 ttgatcaagt tggtttgatc gatggtaaat atcgtatccc aatctatgtg attgaagatt
    25561 atatttggaa attaaaaggc acccggtatc gagaagagga aatcatcgct actttaatct
    25621 actctgtcac cgatcgacac tggcaattgg tagaagatgc cgatctttct tatctgccat
    25681 tgaagtagat ctatttctta tcaaaaaata aaaaggaaac aagaaactag gacatgattc
    25741 tgtctcaagt ttcttgcttc ttttttattt acagctcctg tcgtttatct ggcacaaaaa
    25801 tcgaaggagt cgtttgttgg atccctaata aaacgatgat cgttacaata gctgtactca
    25861 acccgctggc accattcatt acgaaagaaa agagccaagg attcatcccc catagtgcat
    25921 acgctcccca gaaaaccact cctgcaataa aatgccaaaa atatctggaa agtgcgccaa
    25981 tcaatgcacc aaacacgatg ctttttctaa tttgtttcgt attatcgtct acatagcctt
    26041 tttggacatt tgaagccatg agccctgctg cgcctgcaaa agtataggca atcgggtact
    26101 caatcaacac ttgaacgaca cttaaataat aaacttgtcc caacggaaaa tgtaggatgc
    26161 cccaaatgaa acctgccagc atccctgcct ttgtccctct tcttaacgca aaaagagtca
    26221 atggaatctg ccctaatgaa atagaaaaac tgctgccgat ctggatcgga atcaacgaca
    26281 acaccatcgc taacgctgca acaatcgttc cttctaccca aactctattt tttcccataa
    26341 aaaaacttcc tcctttgaat aaaaaagtca caaaggagga agcagctttc gaaaatagtc
    26401 aaacagctgt ttattcacaa ttcctacgct cgcataaccg atacaggtac gaagggttta
    26461 gaaatagatt caatctcagc ccaattggac tcccctttgt gagtttattt attatttatt
    26521 ttttgtttca aacaatggac taactggttt gttttcatgg atacgtttga ttgcatctcc
    26581 cattagtccg ccaacactta cttcatcgat tttgtcgatt ttacgatctt ctggaagata
    26641 gatcgagtct gtaacaacta gacgctcgat agctgaatct tctattcttt gtaaagcagg
    26701 gccggataat actggatgtg tacatgaagc atacacacta gttgcaccag cttctttcaa
    26761 agcattggca gccaatgtga tcgtacctgc tgtatcgatc atatcatcga tcagtacaca
    26821 agttttgcct ttgacttgtc caatgatatt cattacttct gcaacatttg cttttggacg
    26881 gcgtttgtct atgatcgcaa tcggagattt caaaaattcc gctaatttac gtgcccgtgt
    26941 gacaccacca tggtctggtg aaacaacgac aacatcatcg cctttgattc ctttttctaa
    27001 gaaataattg gcaataagtg gcgcacccat caaatgatcg actggaatat caaagaaacc
    27061 ttggatttgg actgcatgaa ggtccaaagt cagcattctt gttgcaccag ctttttgaat
    27121 catgtttgct actaatttcg ctgtgattgg ttcccgtgca cgtgctttac ggtcttgacg
    27181 cgcatagcca taataaggca tgactacgtt gattgttttt gcacttgctc gtttcagtgc
    27241 gtcaatcatg atcagcaact ccatcaagtt atcattgact gggctgctcg ttgattgaat
    27301 aacatagaca tgcgatccac gaatactttc ttcaatgttt acttgaattt ctccatcact
    27361 aaattgagta acagatgatt ttcctaactc gacgccgact gctgctgcaa ttttctctgc
    27421 taatgggcga ttggaattta acgcaaaaat ttttaatctt gggtcaaagt aatgttttga
    27481 catggggacc tccactttct ttttcacaac tctagaattc tacctaataa atagtagtca
    27541 tattttttaa ataaatcaag tgattttgca aaagtcttag tttagataag ggagtttttt
    27601 tgcatatcct tctttattga cttgtcttgc tctcgcaatt gccagcgcat attctggaat
    27661 attgtctgta attgttgacc cggcagcaat cgaactgttt ttagctacct cgacaggtcc
    27721 gatgatattc gtgctagaac caataaagct gtggtcgccg acagtcgtat gatgtttgtt
    27781 ctttccatca tagttgacga agacaacacc acatccgaca ttgatttctt ctcccagcgt
    27841 agcatcacca acataagtca agtgaccgac tttagttcct ttgccgattt gagcattttt
    27901 cacttccaca aagtttccga tatggacgtt ttcgccaatt tcagcttttg gtcgtaaatg
    27961 tgcataaggt cccacatctg catgtttctt gacatggctg ctttcaatga ctgagttttc
    28021 aatgacgacg tgatcctcaa tcctactgtc tacgattttt gaatgtgacc cgatgacaca
    28081 atcagaacca atgactgtat ttccttggat ttgtacacct gcttcgatca atgtgtctgg
    28141 accgatttct acgcctgcat cgatgtatgt cgttgctgaa tcgacaaagc ttacgccatt
    28201 gaccatatgc atctggttaa ttcgctttct catgatctca tttgctttag ctaacgcgat
    28261 ccgatcattg actcccattg attcttcaaa atcatccgtt tgataagcag caactgtatg
    28321 gccttcttct tttaagattt cgatgatatc tgttagatag tattcacctt gagcattgtt
    28381 tgttccaact ttgcttaacg catcgaacaa agcttcgttg tcaaaacagt aagttcctgt
    28441 attgatttct tgtacaagag cttcttctgg tgttgcatct ttttgttcca cgattttttc
    28501 tacgatccca atatggtcac ggatgattcg tccatatcct gttgggtttt ctgcttgtgc
    28561 cgtcaagatc gttgcacttg cattttttcc ttggtgatat tcaaaaagat tgttcagcgt
    28621 ttcagtagtc aataatggtg tatcgccact gataactaac gttgttcctt tttttccttc
    28681 caagaaagaa gcagcttgca aaacagcatg tcctgttccc aattgttctg cttgtaacgc
    28741 atatttactg cgttctccta gttgtgcttt aacttgttct gctccgtgtc caacgatcgt
    28801 aatcacttca tctggtttcg tttcactgac ccgattaatg atatgttcta ccataggttt
    28861 accagaaacg ggatgtaaaa ctttatataa ttttgacttc atgcgggtac ctttccctgc
    28921 cgccaaaata attgcataac gtgcgtccaa ctgcgtcact cctaaatata gtttgctgat
    28981 ttcagtttat cccagtatac ccattttttc aacaaaacaa gtgattttct tttatcccag
    29041 cgttttctgg ataaaaaaat agatagacca taacgggcct atctatttgg taaccggtgc
    29101 tctcctgcca actcctcggt cacaagtcac actactcata aaacatgaaa agctgttttt
    29161 tgaaatcgtt tcttgtgact tagagttaaa cggtaggctt acaacctctt cattaaacgt
    29221 ttttcaatca gctgaccttc ggtattagct caataaacgg caaaatatga ggagcaattt
    29281 ttccgtttat tgagctaata ctcaggacag gacgctgatt tcacaacctc tagataaacg
    29341 gtgttcaaaa gccagtttct cggtattaag ccaaatttat gaaaaaatgt ggaaagccat
    29401 tttcacaaaa tttttcttac tactcgaaac taagcggctt tttcacaacc tcttattctg
    29461 aaaaataatt tcctggcaca accgtaatat ttttcgtttg agtatctaca tctttgacat
    29521 aaagcagtga cgtataatca tcgatggcgc gatgaccgtt gaattttgat tcggcaaata
    29581 cagtgatgcc aacaagttct gattcgaatt cttcaatcaa gctcttcatt ccgttgactg
    29641 ttccgccgcc tttcatgaaa tcatccacta caaggacacg tgaaccacgt ttcaaacttc
    29701 tcttagacag ttccatcttt tcaattcttt cagaagatcc agacacatag ttgacactta
    29761 ctgttgatcc ttctgttatt tttgagtccc ggcgtacaat aacgaatggc gcattcagat
    29821 aataggacac agcttgggca ataggtacgc cttttgtggc tacagtcatt acagcatcga
    29881 ttttttcacc aaggtattta gaagcaatga ttcttccaac ttgacgcagc agttcaggtt
    29941 ctcctaacaa atcagagaga taaacatatc ctcctggaag cagacggtct tgttctgata
    30001 gacgttctgc tagagattca atatattttt tcgcctcttc atatgaaatt tcagggatga
    30061 acaatacacc acctgcagca cccgggattg tttccaaggt tccataccct cgttctttaa
    30121 atgttttctt gatgattccc aaatcttcac tgatagaaga tttagcagat tcataacgtt
    30181 cggcaaagct tgttaaggaa atcaattcat gcgggtgatt caataaatac tgtgtcatgt
    30241 caactagacg ttcactgcga cgtactttca cctattccaa ctccttttaa gaaattttat
    30301 cgttttacat tatataagtt tttcttcaaa aattcgagca ttttatgaaa aaataagaaa
    30361 atagttcggt atttattaaa ataaaaagaa gcctataaaa aatttttcgt ttttataagg
    30421 cttcttttct ttataattta gttatttttt cttctgaagg caaccaatat tcgtttgatg
    30481 atatttacaa tcaggaacaa agcaataaag actaatgtga ttgttgctcc tggaggtgta
    30541 tctagataaa acgagctagt tagtcctgac agcatcccta gaaatcctac acaaacactg
    30601 atagcaatca cgacattgaa gcttttgcct aatcgcatac cgattgctgc gggaagaacc
    30661 atgattgcgg atatcaaaag tgcaccggca atcgggatca tcacagcaat agccacacca
    30721 gtaatcacat tgaacatcat cgacatcaaa tgaacgggta gcccatccac gtgggcggta
    30781 tcttcatcaa aagtcagaac atacatcggc cgtttgaata aaaagaacaa tataccgatg
    30841 gcaacgaata agattcctaa aaagatgact tgctcccatg taatggtcac gatcgaacca
    30901 aacagatagg attggatact tgttgctgaa ttccctccgc tcaaattcat caatactaac
    30961 gctagcgcca aaccaccagc cattaaaatg gcaatggaga tttctgaata ggttcggtag
    31021 atcgttcgca gatactccaa tatcacagct gctaatacaa cgactagcat ggtcgtaagt
    31081 gtaggattaa ggttcaaaaa gaatcctaac gctacccctg ctaaagagac atgagaaagc
    31141 gtatcagcca tcagcgattg tcttctgatc actaaaaaca cacctagcat cggggcgatt
    31201 ccggcaatga agatagcggc aagaaacgct cttctcatga attcatatga aagcaacgcc
    31261 attgagaatc ctcctttctc accagtcgga tctgtctgtc cgcatactgt ttgatatctt
    31321 catgatcatg ggtgatcatt agaactgatt tcccatggac atgcgcacta tgtcggagaa
    31381 gacgataaaa ttcatttctg gattcttcat ccatacctgt tgttggttca tcaagaacaa
    31441 ataagtcagg gtcagtagca aaaatacgcg ctaaactgat tcgctgcttt tgtccgccag
    31501 acaattcacc tattcgccga tttcgcatat cccacatccc tacagcttct aaagcacgct
    31561 gtacatgttg atgatctttt tcagagagac gcttgaacca gcggcctctt ggatatcttc
    31621 ctgaacggac taattcgatc accgtactag gaaaacctgc attgaaagat gcaacttgtt
    31681 gaggaatata accgatactt aatttttttc cttcttgatt tttctcagca atctttatca
    31741 ttccttttga aggtttcagt aatcctaacg atgctttcac caaggttgac ttggctgctc
    31801 cattttctcc agttaaaatg acaaattcac ccgcatctac atgataagag acatcttcta
    31861 gtacaggttc atcatcatag taaaaggtca aatcagatat ttcgatataa cgcaccttac
    31921 aacctccatt ctaaaactta attgatactt tcttttaaag ctttcagatt tttttccatg
    31981 acagaaatat aattttcgcc tgctttgatt tgttcattag tcaagctttc taaaggattc
    32041 aacacttcta gcttcactcc tgtttcattc gacaatgtct cagctacctt cgaagaggca
    32101 ttttcttcaa aataaatcac tttgatgttg tttttcttca caaattcttt tagctcagct
    32161 aagcgacttg gtgtgggttc ctgatctggt gaaacgcccg aaatcgctac ttgttttaag
    32221 tcgtattgat tcgctaaata agcaaaagca gcatgctgag tgacaaatgt gcggtttgtt
    32281 gcatctttta gctcatcgga gtatttttga tccagtgctt caagcttttt gatataagca
    32341 tcggcattct tcgtaaaagt ttccttttta tctggatatt tttcgcctaa ttcttcagca
    32401 atcgttctga cttcttttat agctaatacc ggatcgagcc aaacatgtgg atcgtattca
    32461 tgtgaatgac catgcccttc ttctgattct tcatgttcgt ctgcttcttg cgattctaaa
    32521 cggtcgattc ctttagctgc ttcgatcatc aaggtctgtt tgctatccac tgaatccttt
    32581 aacgaatcca caaaaaattc catgtctgaa ctattataca cgaaaacatc tgcatcggaa
    32641 atttctgctc tttcttttgc acttggttcg aaatcatgcg gctccgttcc agcagggatc
    32701 aaaagtttca cgtttccttc atcccctacg atctcttttg taaaatcata catcggataa
    32761 aaagtcgtaa cgatcgtcaa atcctctttt ttgtctgctt ccttgttcgt atttccacat
    32821 gctgcaaaca gtaaagcacc tatcattacc gtagctgtca aaagatattt tttcatttat
    32881 atgctccttt caatgaacaa atcggaattt tacgatttat taacgtgaac taatgtatca
    32941 aaataaaaaa acacagtcaa tagaaatcca tcaatttctg atttaagcat gtatattctt
    33001 gattaaatcg taaaatggat gagttgctgc tcatatctag ccatttcact acaataaaaa
    33061 actgaagaat accattttag gattcctcag tttttcatca cctattttac ttcttatatt
    33121 ttattgcagg gttcgtacta aataaacttc ctcacaaaaa ccttttaacc cattatatac
    33181 tctctgcgct cttgaataat gttgacacaa ggcaaaaacg gttggtccgc ttccactcat
    33241 caatgcagca tctgctccgt acttgatcat acgctctttg atttgatgga tgactggatg
    33301 ccgctttccc gttatatctt ctaaactgtt tcccattttt ttgatcatct gctgataatc
    33361 ttgctctcgg atggcttcag caagtccgtg gatgtcttga tgttggatgg cttgcgtatc
    33421 tacttctcta aacactgtac gtgtagagac actcattctt ggtttgacca acacaaccca
    33481 gcactgtggc atagaaggta atacttggat ttcttcccct ttgccaccga caaatgccgt
    33541 acttccgtag acacagtaag gaacatctgt accgacttcc aaaccgattt ctgccagttc
    33601 ttctaacgaa cagcccagat tccataattg gttgatccct cttagagcag ctgcacagtc
    33661 tgtacttcct ccgcctaatc cagctgctac tggaatattc ttcttaacaa agatcttgac
    33721 accggttttg atattgtatt tattttttag caataaagca gcttgataaa cattgttccg
    33781 ttcatctacc ggtaaaaatg ctttatttgt ttctacaatg attttgtctt ctgttatttc
    33841 ttcaaaaaat agatgatctg ccaagtctac gctcgacatc accatttcca actcatgata
    33901 cccatcttgc cgtctatata atacatccag tcctaaattt atcttggctg gcgctttttc
    33961 aatcaattcc atcctctcac ccattcgaaa agtctaagtc gcaagtattc tatcataggg
    34021 aaaaataaaa gtatagctta tgtctgccta gtggttgatt ttgtcatcgt ttcaacagct
    34081 ttcacagaat tttctcttca taatccaaac aagttctagt gattcttata attctgtgag
    34141 cacttcttct gccaaactcg gaacatttcc tttttctttt cctatcagac tgagtagctg
    34201 catggtagca aatccgaaat tacttccttt cctataagca aaataacgtc cttcccaatg
    34261 atcgacatat tttaatttct tttcataagc agactggaag gaatagaaaa aattaccata
    34321 attatatacg atatgcatga ctttttcttg aaagaaagaa aaatctgttt ctccaacatt
    34381 agctaatgga gctaagccta gattggcttc cgagtagccc attttctcgt atacaataaa
    34441 cagattggct aacagatagt cagctaattc ctcagattct ttaggtaaga cccgcaacaa
    34501 atcatatccc gcctgcttct tcgtcatcgg ctgagcggtg atgaagccaa cgactttttg
    34561 atctttacgg acaataccga caccgctaga taatacatat gatttatcaa atcgtcccac
    34621 agagaagtat ttttctcgtg caccttgaag ccatttttgt gaaacttcat ttacagcttg
    34681 atgtaattct tccggcagtt gatcgtacca agtaaaatgg tatccttctt tcatcaaacg
    34741 ttgatattca agttgctgac tcagccgaga caagtcttct gttgctagat caacaatccc
    34801 tgcttcacct actttcgtga agtgaaaacc caattcatga agcatcacta cgtagctttc
    34861 tgatatctta taaaacgcaa gctgatagcc taaacaatct gcttgtttca taaactgatt
    34921 cgttgctgcc cgcatcattt taggattgcc aactggatct cctaaaacaa agcatttgtt
    34981 tcctttgatc tgataaccaa aaacgacttg atcttggtcg tcttcttggt aataaaaata
    35041 actgtaacct gacaagcgaa gctgatggct tgtagctgtg cctccgtatg tttccaataa
    35101 acggtcaaaa cggtcttttt cgtactcact gccaagcttc gtagtatctt cagctaaata
    35161 ttgataaagc gccactaaag ctagcaatga gatgcccaaa ccaataagcc ctgagaacca
    35221 cacatcatct gaaggaaaca aaataaaatg atttcctgtt attcgaccga ctaaaaagcg
    35281 tccgctgtga tagcctgcaa ttgcgtaaac gataaacaaa aagccgaaca agctagcatc
    35341 aaagattata gctccccaag aataaacgaa ttttgttcga taaaattctt ttctggataa
    35401 ccacacagct aacagaatca agacatatac caatatcaac tgccaagata acgtacgtgt
    35461 gattgtattt aaaataccaa aagatagtaa agcaatcgta ggcagaaatg cctttttgac
    35521 ttttactgaa agtgctctag caaggccgag taataaaaag ccaaccaata gattcactgt
    35581 ctgatctaaa aaattgaatg agaacggcaa taatacttga aatagccgac tgattgcaga
    35641 cagattagtg attgttgata gaagtaccat catgatcccc gcaaaataga cagcacttac
    35701 gagcacgata tgcgcaacct tttggaaaaa cagtcgcgga atgttatcta aaaatcgatt
    35761 gattttgact cctgtatgtg tgatgaacaa tagaagtccc gtcataaaag gtagaacata
    35821 ataaaacaac cgataatata atagccaaac tactgctaca tcttgccgaa cagacaattg
    35881 actcattcct aagatcatca aaacgtcaaa agtccccatt ccacctggga ccatcgtcag
    35941 cattcctatc aatgtagcaa tcatgaacat aggatagatc gaaagcatcg aaatatctat
    36001 tctcatcaac ttgccgatga tcaaaaatac cgcaagggct cccgtccatt gtccaaagga
    36061 tgccagtagc agaccaagca agccttttgg ataaaactcg aagaacaatg tctttctctt
    36121 tagataagca aaaaagaaaa tgactggtgc taaaaaacta ccgccgaaaa gccagatcca
    36181 atagcttcga aaaatacttt ctgactgaat aaaaaagaca tcaataaatg taagaaacga
    36241 ccatacagac aaaccagtca gcataaatag tgcgactttt gatacagtgg ctaaaaccat
    36301 ttttctattt gtctgctttc cataaaaact agcccgcaga cttgctccta ccacaccacc
    36361 aaaaccggcg agattgttga tcgtgtttgt tacccacgca gcaagaagaa actcttttct
    36421 ttccattttt ggttttcctt gtttttctaa gactctgatc gtcacccaat catacaacaa
    36481 catcggagtg acacccagca gtccagccag aatcatgagc aggatcgtca tgcggctttg
    36541 ttgtcccatc ctttgccaaa tatcttgcca actcatgcca ttagcaatat ttgcgacttg
    36601 atttgccaca aagactaaca cggagccgaa aaaaatcagt tttaaaatca gactatgttc
    36661 ctttatccat tgcagcatca agtatcgttt atttttcaca ttttcccatt cctaacttgt
    36721 atacttttgt gtcatacccc aatgttatct tatttatttt aatttttcca ctttagacct
    36781 gttctttgct tgcgatgata gaaaaagaaa gatatcctaa tgctaaagga gaggataaca
    36841 tgaaagtagg atttatcggc acagggaata tggcacaagc tattattgat ggtttattaa
    36901 aaaaaggaac agtctcgaaa gaagaggtgt tcgtttccag tggacatttt gaaaatgcac
    36961 aaaaatttgc tgctgaaact ggcgtccatg catgcaaaag taatcaggaa gttgcatcac
    37021 aatcagatat cgttctttta gccgtgaaac caaaaatcat tgaaaaagta ttggaagaac
    37081 taagagaata tagccagaaa gtcttattca tttctattgc ttcaggaaaa tcattaatag
    37141 atttagaaac agcgcttgaa gatcctcagg cctccatcgt acgtgtgatg ccgaatgtca
    37201 atgtcagcgt aggagcaggc gtttctgcga tttgcaaaaa tgaagctgcc acggaggaac
    37261 agcttcagca agctacgatg atttttgaag caatcggcag cgtctatcct ttagcagaat
    37321 cagatttcag tacattcatt ggccttgctg gcagctcacc cgcattcgtt tacatgctga
    37381 tcgatgcgat gggacggact ggtgtactga atggtttgtc caaacagaca gccactgaga
    37441 ttgctgcaaa agcaattatg ggttcttgtc aaaaattcct ttcaagtgat aaaaatccat
    37501 gggaacttgt agaccaagtt tcttctcctg gcggtacaac tgttgcaggt gtagttgcct
    37561 tagaagaagc aggattcatc ccagcaatca tcaaaggaat caatacgacg atcgagaaag
    37621 atcttgagat gttgaataaa aaataatgat agcaatgttt tttcgtgcct tacaaaaaaa
    37681 gaagagctgt ggcttttgcc gcggctcttc ttttgtattt tttattattt agcataatca
    37741 gttgcacgag tttcccgaat aacgattact ttgatgtgtc caggataatc cagttcttct
    37801 tcgattcgtt tacgaatatc acgtactaaa cgaacagcgt ctaaatccga gatttcttct
    37861 ggcttgacca ttacccgaac ttcgcgtcca gcttgaactg cgtaacttga ttctacaccg
    37921 gcgaagctgt tcgaaatatt ttccagactt cttaaacgac ggatgtagtt ttctaatgat
    37981 tcacttcgcg caccaggtct tgctgctgag agcgcatctg ctgcagctac aaggacagag
    38041 ataacagaag tagcttccac atctccatga tgagaagcga tcgcgttgat tactactggg
    38101 ttttctttgt acttggcagc taattccgca ccgatctcaa cgtgagatcc ttcaatctca
    38161 tggtcaagtg ctttcccaat atcatggagc aacccggcac gttttgctaa ttggatatct
    38221 tcgccaagct ctgctgctaa gataccagct aattttgcaa cctcgaccga atgcttcaag
    38281 acgttttgtc cataactagt tctgaatcgc agacgtccga tgatcttgat tagatctgga
    38341 tgaagtgtat gagcaccgac ttcaaacgct gcctcttccc cgtattcgcg aatacgttca
    38401 tccatttctt tacgagattt ctcgaccatt tcttcgatac gcgctggatg gattcgtcca
    38461 tcttgtatca gtttttccaa ggtcatccgt gcgatttctc ttcggattgg atcgaatccg
    38521 gaaagaacaa ctgcttccgg tgtatcatcg atgattaaat cgataccagt cagcgtctca
    38581 agcgttcgaa tattacgacc ttctcgtcca atgatacgac ctttcatttc atcattcgga
    38641 agggtaacga ctgaaacagt cgtttctgaa acttgatctg ctgcgcagcg ttgaatcgct
    38701 aatgataata agtttttcgc tttgcgatct gcttcttctt tggcacgctg ttcagattct
    38761 tttaccatta gagttaactc atgattcagc tcttcttcag tcgatttcat gatgacatct
    38821 cgtgcgtcct cttttgataa accagcaatt ttttctagtt ctttttgttg atcttcaatc
    38881 attttttcaa cgtctttttc tcgctcttca attaattgct gttttgaact aagtgcttct
    38941 tctttacttt caagtgagtt ctcgcgtttt tctaacgagt catctttacg attcagcgtt
    39001 tgttcgcgtt gaatcaaacg attctcttga gattttaatt ctaacttact ttctctcagc
    39061 tcactttcaa cttcagaacg gtatttctga ttttcttcct tagcttctaa caatgcttct
    39121 ttttttagag tttctgcttc tttttttgca ctatcgatga ttccagcagc tgatgattgt
    39181 gcaccagcta tttctttctc gtgacgtgac tttgcgacca taaaccctaa accaagaccg
    39241 acaattaaac cgatgatagc gaggagaatg ttgaatacca taaaatccac ctccatacta
    39301 tctttttcat ttttatcatc tatacagctt ctatgataaa ttattctaaa ctcgtcaata
    39361 tgcatcttcg catatatgtg tacaaaaaac atgcacaact ttattctaat gtttgtaccc
    39421 cgtagtgtca actttaaata aacaaagaaa cggctttcta cttattcatt aaacgatggt
    39481 ttttaaaata aatgacacta gtttcatata ggtaaacctt taaattgaga aacttttcta
    39541 ttaaattatt ctttgtagat gaacagatat ttttatttta tgtacccact cgtataatta
    39601 aataactcac gatttcttta ttcaataaaa aaacggcagg tcaaggagag tacctactct
    39661 tcagaccagc cgtttttgtt tatcataatt tttattcttc tactaaaggc aattcttctt
    39721 gtcctttttc atcttctggg acagcgactt cttcaccaat accataagct gctctaacta
    39781 gagcagaaac ttctgccatc atttcaggat gattagccat atagatttta gcgttttccc
    39841 gtccttgacc aatccgatct tctttataag aataccaagc accgctttta tcaacaatgt
    39901 ctttttctac tgccatgtct agaagttctc cttcttgtga gatccctaaa ccatacatga
    39961 catctacttc agcgattttg aaaggaggtg ctactttgtt ttttacgact ttgatctttg
    40021 tacggttacc aacaatatct gtaccttgtt tcaattgttc tgcacgacgt acttccaaac
    40081 ggatcgttgc gtagaatttc aatgcgcgtc ctccaggagt gatttctgga ttcccaaaca
    40141 tcacgcctac tttttcacgg atttggttga tgaaaatagc gattgttttt gttttgttga
    40201 tcgaaccaga gagtttacgc aatgcttgag acatcaaacg tgcttgtaac ccgacatgtg
    40261 agtcacccat ttcgccgtcg atttctgctc gtggaactaa agcagcaact gaatcgacta
    40321 ctacgatatc tacagcccca cttgatacta aagcatcagc gatctctaga ccttgttctc
    40381 ctgtgtcagg ctgtgaaaga agtagttcat cgatattcac acctaatttt tgtgcatatt
    40441 gcggatctaa cgcatgctca gcatcaatga aagcggccgt tccgccattt ttttgtactt
    40501 ctgcaatagc gtgtagtgca actgttgttt tacctgaact ttcaggtcca tatacttcga
    40561 tgatacgtcc acgggggtaa ccgccgacac ctaaagcgac atctaacgca agagagccac
    40621 ttggaattgt agagatttgt tggtcgattt tttcgcccaa tttcataata gagcctttac
    40681 catagctctt ttcaattttt ttcagtgcag catctaaggc tgctttacga tcatctgcca
    40741 atacgataat cctccttata tttacaaatc agagcaacat ttcattatcc ttatcatatc
    40801 ttttttagtg aaaaaaagca agaaaaaacc gaacaaatat tcgcatttat cattttctta
    40861 aaagctgttt tctcaaaaga tcaaatcctt gcataaccgc attatatctg atagcagcac
    40921 gatctctggc aaagtgatat ttttccgaaa ccacgccgtc tttagatgaa atagcgatcc
    40981 atactgttcc tgcagattga ccttctaacg tgtccggtcc agccactccg gtgaaagcta
    41041 cactgtaatc tgttcttgct atctcacgtg cttgaatggc catctgctcc acacattctt
    41101 tgctgactgt tccatattct gctaatagct ccggatcaat ctccagaaaa ccagcttttg
    41161 tttggagcga gtaggtaacg aatccgcctg gaaatacttc agatacacca gcaatgtcgc
    41221 ctaatgcggc ttggaaggcg ccggctgtca aactttccgc tgctgttaca gtctgcttgt
    41281 tttctttcag tagttccact acgacttttg ctagcgaatt atcatcgccg tatccataaa
    41341 aatattcccc tactcgttct tggatttttt cttctaatgc taaaagagct tgattccctg
    41401 cctgaacatc atttgttttc actgtcagtc gtaatgtcac ttcatttggt ttggcatatg
    41461 gtgcgatcgt tggattgatc tgtgtctcaa tcagatcttt tagctcagtg accagtcgcg
    41521 attcaccaat cccataaaag cgcaaaactt tagaaattag tttttcttca gaaggaaaac
    41581 gttcctctag taaaggacgt acttgttcca caaacattgg tttcaactca ttaggcggac
    41641 cgggaagaag gatatacgca tgtgtatctg tttgataaaa agtccctaaa gcaagccctg
    41701 tacgatttgg tagaggaacc ccgccttcga taatctggga ctgttgcaga ttgtttttgg
    41761 tcatttttcg atgggttgtt tcaaaatatg ccaataattt tttgtatcct tccgtatttt
    41821 gtatgagaga cttaccgagg tgagccgcag ttgcttcttt tgtcaggtca tcttctgtcg
    41881 gtcctaatcc gccacataag agtatgagtt cacttcttgt ttcagctaga gcaatcaact
    41941 cttctaaacg ctgctgatta tctcctacaa ctgtttgata ataaacttcg atacctagat
    42001 cagctagctg ttctgacaag aaagtggcat tcgtattcac gacttgtcct agtaaaagtt
    42061 ctgttccgac agctattatt tctgctttca ttacttcccg ctcccttata tagtatacgc
    42121 tgtattttta tattttttta tttaaaaaga gggcaaggag ctgtgacaaa aatcttgtca
    42181 cagctccttg ttccaaataa acagtgttct cctgccaaaa cctcggtcat aagtcacaat
    42241 atacataaaa catgaaaagc tgttttttga aattatccct tgtgactcag agttagacgg
    42301 caggctcaca acctctccac taaacgatac ttacattgat cctttgaaca caccgctgtt
    42361 tttgatgaag taatccacac ctgaatagat agtaaagatc agacaagcat aaagcatgat
    42421 ttgatctact ggaataccga tcaggctgaa tgggatatta ttgataaaca gcaagataat
    42481 agcaaccatt tgtgtagccg tcttgacttt tcccggccaa gctgcagcca tgacttctcc
    42541 atgttcgaca agtagtaaac gcaagcctgt gacggctaat tcacgacaga caatgatcgc
    42601 tactacccaa ccaggggcct ttccttggtc aaccaacatg ataaaagcag tcataactag
    42661 cattttatct gccaacgggt ccgcaaactt gccaaaatta gtgacaaggc ctcgcgcacg
    42721 ggcaattttt ccatctaacc agtctgtgat actagctaaa gcaaaaataa ctgcacctac
    42781 tagctgtgtg acagccagtg tggtgtctcc tacgttaaat tgtccccaat ctaaaggtaa
    42841 acctacaacc aaaatgaaaa tcgggatcat acaaattcta agtacagtta atttatttgg
    42901 taaattcaca ctatcgactc ctttcttccc tattctacgt gactatcagc gaagtttcca
    42961 ccgatattat ttacttttcc aaatgaattt attcaaagga gtatttttat tgaaattgat
    43021 ctttttgaat taacgaagaa ggaccgcgac acagatttgt cacgatcctt cttttttata
    43081 cctacaagta aacagatgcg taaactaatt tatacgatca ttctgcgtat tgaagagtaa
    43141 ggttcacatt ttttggactc tgattgtttt cccctggatt aactggtacg gattctccat
    43201 tcgctttgat cgttgcattg cttgctgcac caatcgtgat ggtcgcattt gttgctgttt
    43261 ccggtaacac ggtactctgt gtttcatttg ctgccagtgt cccttgataa acataggcat
    43321 tgtctactag cacacctaac catacacgat tgacagcagt aaattcaagg gttacaggct
    43381 tggtagcgtc tgtgatattt actgcgatgg cactgccaga atcttctcct gtagctattg
    43441 ccattttctt ttcttcactt gattttgttg ttgattctac tgtagaagac tctttggtct
    43501 gcgtgctttg ctctgaggaa gaggactcct cgactgatcc ttctacttta agcgaagaag
    43561 ctgtgtctcc gattattgga tcagcttgac gatcttgcca agtcatatag cccacgacaa
    43621 cgacaatcgc caaagcaacc aatcctaata aaatgacagg aagacttgtc caaaagcgac
    43681 ttggatgcga ttcttcctga tgcattgctt ttctagaagt acttactgtt tcaggctgcg
    43741 gacgttcagg gaccgatcct gtcaatttgc ttttcccatc aaaaacatcg actaaatggt
    43801 ctccatcttc tcctacagct tcagcgtatt gccgaataaa tgcccgcacg taaaatttac
    43861 caggcaattg gtcaaaatca tcattgtcga ttgcttccaa ataacgtttt tgaatttttg
    43921 taatctgctg taattcatct aaagatattt ttttattcag gcgtgcttgc cgtaattttt
    43981 ctccgatggt ttcaaaagcc actcgttcat cttctccagt ctgtttgatt ctccgaagca
    44041 tctgctgttg caacgaaaga cgttatgttt gggtgctgtg acaaaagtca tgtcacagct
    44101 ccttttttcc gaataaacat tgttctcctg ccaactcctc ggtcacaagt cacaatatcc
    44161 aataaatata agaagctgtt ttttgaactt gttccttgtg actcggagtt aaacggcagg
    44221 ctcacgacct ctttcttaaa cggtgttcaa tcagctgttc ctcggtatta gctcaaaaac
    44281 cggcaaaata taaggagata tttttccgta tgtttctcta atactcaggt caaaacgctg
    44341 atttcacaac ctcttcctta aacggtgttc aaaagcttgc atctgcggta ataagtccaa
    44401 ccaagttaaa aacattaaga agctgttttt acttggctgt tcttattact tgatgcaggg
    44461 cagctttttc acaacctctt ggtacacggt gttccagaag tagcttcttc ggcaataagc
    44521 cgaaatatca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct
    44581 gaccacttct gtcacaacct ctttgattac ttcgtttgat tttattttaa tataagttga
    44641 ttaaaagaga gcatgatttg ttttattttc tcactaattt ttccaacaca actaagaata
    44701 ccataaaagc tgattttgga ggaggttcct actacaagtc taaaacgaaa tttaagactt
    44761 ttcctcataa aatactggtt tattttggtc tcatgtaaaa ctcgctgaag gcctcttcac
    44821 ttataaatgc ttccccagct gccagtacgt ctttcatttg gatcgaatcg atgatttcag
    44881 gaagatcaaa taaagttcga tcgccaaata aactttgcgt aaattgattg gcaatatatt
    44941 cgatcgaatt taatgattgg aaatattgtc caagcatttt cttcttcagc aaatcgagat
    45001 ttgtttctga cacttccgga tcatcagcga atccaagaat gatttctttg actttttctg
    45061 ctgctttctc tggttcgtcc gtatctccgc tgaagtccgc aaagtgaaat tcacgatcca
    45121 agctgaattc aaaaccaaag ctgtcatcga taattccttg attgtacatc gctagatagt
    45181 ttcgagaagt attgcctaaa atcatttgga acaacagatt gattgctgtt ttatagcgta
    45241 acagttctgt tccttcttgc ggcaatgtgt ctaatccttt aataccaagt acaaatttat
    45301 ctcgtgtaat ggctgcttcc aatgcacttt gtttgatgat ttcttttgtg ttttcaggga
    45361 aataacgtac aatttcttgc tttggaggga aattttttgc ttcttggttt tcccgaataa
    45421 gtttcatcaa tttctcaggc tccatcttac caactacaaa taaaaccata ttgcttggct
    45481 gataaaacgt ccgatagcac gtatacaagt cttgagctgt gattttgtcg atactttcaa
    45541 ccgtgcccgc aatatcgata tgcaatgggt gtgtaggata caagttattt agtatgccaa
    45601 aaaacatccg ccagttaggg tcgtcttcat acatttggat ttcctgaccg atgattcctt
    45661 tttccttgtt tacagtttct tctgtgaagt aaggtgcttg tacaaaatcg atcagagtcg
    45721 tcagattctt ttctacctga tccgtcgtag aaaacagata gctggttttc gtaaaacttg
    45781 tgaaagcatt ggcggaagct ccttgtttcc caaataattg aaagacatca ccatcttctt
    45841 tttcaaacaa cttatgttct aaaaaatggg caattccatc tggaactttc accttttctt
    45901 tttcaccata gggaataaat tcgttatcga tcgaaccata attcgtactg aacaaaccat
    45961 atgtcttgtg atagtcattt ttaggcaaca aatacacggt caaaccgttt ggcagtactt
    46021 catgataaag tgtttcattg atttgctcat attctgtttt attcatttgc atttcctcct
    46081 gctaaaaaga agattgcctg caattcgatt tgctcagcta cttgttgtat ctctttgatc
    46141 gttacgctct ccatacgttt caaccattct tcatcagata attttgtttc cggcagccag
    46201 ctgtccaaaa aagaagcttc gattgccgct tgcggagagt ctagagaaag cagatattgg
    46261 tttctcagca ttgcttttgt ctgagacagt tccaactctg ttatttcacc atttcgcaaa
    46321 ctttctaatt gctcgtgaat cagatgaagt acttgatttc ggttcttttc atcgataccg
    46381 gtttggacac tcatgaatcc tcggaacgta tccacgctac tagaggcata ataagctaga
    46441 ctttctttct ctctgacatt catgaaaagt ttagagtgag gaaaaccgcc aaataaaccg
    46501 ttaaagacca ttagcgcaaa acgttctggt tcatcgtaat acacatttgt ctgataagct
    46561 aggttcagct ttgcttgaac gattggctca cgtacttgtc gttccttcac gatattcact
    46621 tgaggttggg tataaaacag atctggataa actgtttccc gcgactcaaa tggtaattgc
    46681 ccaactgctt cagctgcttt gtccggatca acatcgccga caacaaaaat gtcgatttga
    46741 tcatttgcca tcatttcttg atagtaggac gctagcgaac tagcagtgat cttgtccaaa
    46801 tcttcgacta cgcccaaccc tgggatcttt tgttccgggc tgttttcaaa ataaagttct
    46861 tgcaaggcat aggaagcaaa agtttgttta tcttccttca aactttccaa atatagacgc
    46921 agattgttct tttccaagtc aaacgtcaac tgatcaaact gatgattttt aatatttgga
    46981 taaaataaaa cctcttttaa aaaatctacc gcttgagaaa aaaggtttgg atcattgaca
    47041 tattcaccat tcacaaaact gatccctgcg tttagccaat gtaaatttcc tttttttcta
    47101 actgataatc caaagcttgc gccataaagc tctgcaagtt gcgcacttaa ctttgtctga
    47161 tccgaataat tcaaactatt tgtttccaac atgctgctga gaagtgaacg tttcgtgatg
    47221 acttcttgtt gcagacgcgc agtaaaacgg ataaaaatcc ggatcgtttt gtattgtttt
    47281 gttggtaaga catgaagatt cacgccttct gccaattgat acaccatatt tatatgctcc
    47341 ttttcacagt tgattgcccg cttactcttt taatgggtca acctgccttt tttacttatt
    47401 cctctgcttg aaatttataa gttcaggcaa ttcgccgttt tttctaaatg atgttacact
    47461 ttatagtata gcatagatgt tttttatcaa aagaaacaga cggctaaaag cgtatattgt
    47521 ttttttattt tttatttttc gatatactaa agacaaataa aagaagggag ggtcattcat
    47581 gatcaaagaa ttcaaagagt tcatcatgcg tggagatgtc ttggatcttg ctgtcggcgt
    47641 tgttattggt agtgcgttta caagcatcgt aaaccaagtg gtgcaaggct tgatcacacc
    47701 actaataggt tggtttgtcg caatgatcac aggtacttct gatttagaag gagcactttc
    47761 aattcttgac tggtcgccga caaaaggcgt cacatttgcg tttggtaacg ttattagtgc
    47821 tatcattaca tttctgatca ctggttttgt cttattctta gttgtcaaag cggcaaaccg
    47881 tgcgaagaat ttacgctcca aagaagagca agtggaagtg gaagaaactc ctactgcaga
    47941 agattatctt agtgacatac gcgatcttct tgccaaacaa gtggcagaag acaatacaca
    48001 tccagtagag caaacaaatc tttccgatac ttctaaataa taaagaatct aaaaaaaaag
    48061 aaagccgatt ttcagtgtcg gctttctttt tttagattct taacttgcta cttgttcctt
    48121 tttcatgtcg atatgtggaa tatcatcttc caaatacaca tctgagattg ccttgaatcc
    48181 aaacgactca taaaaagctt gtaggtattc ttgtgcgccg atcacgattg gtttttctgg
    48241 aaaatttttc tggatcatgt taatcgtttc ttgaacaatc tttcttccta aaccatcttt
    48301 acggttgtcc tgatgaacaa ggacacgtcc aaaatggata ctctccgctt cttcatatac
    48361 tcgcgtgtag gcaaggatct tcccatcagt atcttgaaaa aacgtatgcc aagcttctcc
    48421 atcaatgtca tcaacttctt gataaggaca ctgttgttcc acaacaaaaa ctgcgattcg
    48481 ttctttgacg atcgcgtaaa actctgctgt tgacaattct tcaaatttct ttacctgata
    48541 ttccactaag caaacgcccc ctgctttttt attcttagca taccgattcg cacacaaaaa
    48601 agcaaacgaa ccttacgaat ctcaggctcg tttgcttttt aaactgcttc agttaaaaat
    48661 tcgatctcaa ctgtccgagt aagtacatct gaatagctgt aagatactcg ttcaaaagaa
    48721 ttttcttcag gatccaaatc aacaacgaat actgaaggat aggtttctgt taaaacgccg
    48781 cggcgttctg tttgacgctt tcttcctgtc tgagcaacca acatgattgg gctgccgata
    48841 cgaccttcta aatctttctt gattgaagct aaagttgttg gcatacagtt cacctctact
    48901 atcaactata ccacatattt t
//
LOCUS       DACDTO010000013        47952 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDTO010000013 DACDTO010000000
VERSION     DACDTO010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.013"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(38..1189)
                     /locus_tag="IWK70_000777"
     CDS             complement(38..1189)
                     /locus_tag="IWK70_000777"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9613345.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="IWK70_000778"
     CDS             complement(1202..1639)
                     /locus_tag="IWK70_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9613346.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="IWK70_000779"
     CDS             complement(1654..3420)
                     /locus_tag="IWK70_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9613347.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="IWK70_000780"
     CDS             3684..4361
                     /locus_tag="IWK70_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613348.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4528..5490
                     /locus_tag="IWK70_000781"
     CDS             4528..5490
                     /locus_tag="IWK70_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613349.1"
                     /translation="MIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEAT
                     YHYEKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYE
                     NIFSGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAA
                     MLSLVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLF
                     LSTISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVI
                     IGGDIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5690..6130)
                     /locus_tag="IWK70_000782"
     CDS             complement(5690..6130)
                     /locus_tag="IWK70_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9613350.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6141..8174)
                     /locus_tag="IWK70_000783"
     CDS             complement(6141..8174)
                     /locus_tag="IWK70_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9613351.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8176..9879)
                     /locus_tag="IWK70_000784"
     CDS             complement(8176..9879)
                     /locus_tag="IWK70_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9613352.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IWK70_000785"
     CDS             complement(9973..10923)
                     /gene="manA"
                     /locus_tag="IWK70_000785"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9613353.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10962..11987)
                     /locus_tag="IWK70_000786"
     CDS             complement(10962..11987)
                     /locus_tag="IWK70_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9613354.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12196..13065)
                     /locus_tag="IWK70_000787"
     CDS             complement(12196..13065)
                     /locus_tag="IWK70_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9613355.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13524..15416)
                     /locus_tag="IWK70_000788"
     CDS             complement(13524..15416)
                     /locus_tag="IWK70_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613356.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15413..17140)
                     /locus_tag="IWK70_000789"
     CDS             complement(15413..17140)
                     /locus_tag="IWK70_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613357.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17156..17806)
                     /locus_tag="IWK70_000790"
     CDS             complement(17156..17806)
                     /locus_tag="IWK70_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9613358.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18010..18306
                     /locus_tag="IWK70_000791"
     CDS             18010..18306
                     /locus_tag="IWK70_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9613359.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18478..19098
                     /locus_tag="IWK70_000792"
     CDS             18478..19098
                     /locus_tag="IWK70_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9613360.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19190..20356)
                     /locus_tag="IWK70_000793"
     CDS             complement(19190..20356)
                     /locus_tag="IWK70_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9613361.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20368..20721)
                     /locus_tag="IWK70_000794"
     CDS             complement(20368..20721)
                     /locus_tag="IWK70_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9613362.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20849..21871
                     /locus_tag="IWK70_000795"
     CDS             20849..21871
                     /locus_tag="IWK70_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9613363.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(22310..22744)
                     /locus_tag="IWK70_000796"
     CDS             complement(22310..22744)
                     /locus_tag="IWK70_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613364.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22854..24713)
                     /locus_tag="IWK70_000797"
     CDS             complement(22854..24713)
                     /locus_tag="IWK70_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9613365.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24831..25991)
                     /locus_tag="IWK70_000798"
     CDS             complement(24831..25991)
                     /locus_tag="IWK70_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9613366.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IWK70_000799"
     CDS             complement(26298..27722)
                     /gene="araA"
                     /locus_tag="IWK70_000799"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9613367.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27738..28436)
                     /locus_tag="IWK70_000800"
     CDS             complement(27738..28436)
                     /locus_tag="IWK70_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9613368.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28393..30054)
                     /locus_tag="IWK70_000801"
     CDS             complement(28393..30054)
                     /locus_tag="IWK70_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9613369.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30272..31672
                     /locus_tag="IWK70_000802"
     CDS             30272..31672
                     /locus_tag="IWK70_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9613370.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31924..33003)
                     /locus_tag="IWK70_000803"
     CDS             complement(31924..33003)
                     /locus_tag="IWK70_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9613371.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33089..34048)
                     /locus_tag="IWK70_000804"
     CDS             complement(33089..34048)
                     /locus_tag="IWK70_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9613372.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPAITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34060..35583)
                     /locus_tag="IWK70_000805"
     CDS             complement(34060..35583)
                     /locus_tag="IWK70_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9613373.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35593..36156)
                     /locus_tag="IWK70_000806"
     CDS             complement(35593..36156)
                     /locus_tag="IWK70_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9613374.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36162..37025)
                     /locus_tag="IWK70_000807"
     CDS             complement(36162..37025)
                     /locus_tag="IWK70_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9613375.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37039..37935)
                     /locus_tag="IWK70_000808"
     CDS             complement(37039..37935)
                     /locus_tag="IWK70_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9613376.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38110..39381)
                     /locus_tag="IWK70_000809"
     CDS             complement(38110..39381)
                     /locus_tag="IWK70_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9613377.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39371..40888)
                     /locus_tag="IWK70_000810"
     CDS             complement(39371..40888)
                     /locus_tag="IWK70_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9613378.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41086..41976
                     /locus_tag="IWK70_000811"
     CDS             41086..41976
                     /locus_tag="IWK70_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9613379.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IWK70_000812"
     CDS             complement(42049..42873)
                     /gene="nadE"
                     /locus_tag="IWK70_000812"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9613380.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42885..44357)
                     /locus_tag="IWK70_000813"
     CDS             complement(42885..44357)
                     /locus_tag="IWK70_000813"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9613381.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44994..45356)
                     /locus_tag="IWK70_000814"
     CDS             complement(44994..45356)
                     /locus_tag="IWK70_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9613382.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45579..46331
                     /locus_tag="IWK70_000815"
     CDS             45579..46331
                     /locus_tag="IWK70_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9613383.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46471..47394)
                     /locus_tag="IWK70_000816"
     CDS             complement(46471..47394)
                     /locus_tag="IWK70_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9613384.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47409..47681)
                     /locus_tag="IWK70_000817"
     CDS             complement(47409..47681)
                     /locus_tag="IWK70_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613385.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13980 a         9954 c         8269 g        15749 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt ttttgatcat aaacttgctt atacagaatt cgatgaactg
     4501 aatcataact atgaagagat gcaacgtttg attgaaacgt tgaaagaaga tgagaatgag
     4561 aaagaatttg tggaaagttt agagaaaagc aacaaactga tcgaagcaat cttacatgca
     4621 aaaaacacag ggaatgtcca gcaaatggtc gaagcaactt atcattatga aaaagatata
     4681 ctggatcgac tgatttctgg tcaaagacaa ggaattccaa tcattgaaca acaaaaacgt
     4741 gtcgagttgc tccgttatat gaaagaacat cagatccaaa gatattctat ttttgacttg
     4801 ccagcgcatc tccctttagc aaattactac gaaaatattt tttcaggaat gatttctagt
     4861 tttttgattt tatgtataac cgccttgttt ctttcttcca tcatctctta tgaaaaaaga
     4921 aagcaggtaa tctctctagt caatctatta ccggatagta tggtaaagaa acatagcatc
     4981 cgttttacca tttattatgg tgcagctatg ctaagtctcg tgatgccatt tttgatcgtc
     5041 tctatactag taataatcaa aaatggtttg ggagacttcc gttatcctgt aggaacgatc
     5101 attggtcagg agatacgaat ccttccgatg tatgagtatc ttttccagag ctttctgttc
     5161 ctgttgttgt gggtgctctt tttaagtaca atcagttttc ttctatctgc tttatttgaa
     5221 cattctctag tcaatctttt aggtacactt ctttgtcttt tcttagcaga gtatcgcttg
     5281 ttttcttcga ttggatggat agaatcaatc agtcattact tgccaacttc ttatgttgat
     5341 tttcaaaatg tgatcatagg aggagatatt ttctcgccac tagcttcaga acaggttact
     5401 ttcatgaatg gtatcttgac acttggtata tggagtatcg ttttattatt cataggtatg
     5461 ggcactatct atataaaaaa gagctattaa aacacaaaaa taataggggc ggtgacaaaa
     5521 accttgtcac cgtcccctat ttcaaataaa cggttttcta gaataaatag ttttcaaaag
     5581 cttgcatccg cagtaataag aacctcccaa caaaaatagc tcctcttatt tttatcttgg
     5641 ttgaagttat tacttgatgc aaggcagctt tttttactgt gaacattggt tatatatgtc
     5701 catgttcaaa aatcatttgg acttcttcaa tggttttcgc atcactgagc gccattactt
     5761 ggtcgatgtc tgaacagaaa aaggctaaat cttggagcac cgctaactga tgttcttttt
     5821 ttaaagccac tacaaataag agtgtcgcga tttttggttc ctcttttgtt ccaaagtcca
     5881 caccatcagg cacttggact aaatagattc cttcttctac tacttcttca tctttgtcag
     5941 tcccatgtgg caaggctacg aaattcccga tatagacact cgctttttct tgtctttcga
     6001 tcattgattg gatatatgcc ggtttgattc ccccttgttg ggtcatccac tggccgatct
     6061 cggtcaacgc ttctttccaa ttttgatagg attgatcgag caatattttt tctgctggta
     6121 gctgtatcat cttttcacct tcatttcttt atttcttcaa tcaggatttt cgctaacaac
     6181 tgataaacga tttctttatt tcctgagcgg aagatctcgg tgttcaagtc attcatgatg
     6241 attgctccac tgatctttcc taaaagaatc ccgtcttttt cttggattgg cgaaggcgcc
     6301 agcataacta acatacggga caaatcgact ggctgtctat ccatcccttc aatggtcaac
     6361 ggatgtttca aattgaaaat acaaaagatc ggtttagaaa tcaatgagct ggatgtatgg
     6421 aataacccca tatttgtatg aggaataccg ataggtgctt gttcatatcg cttaagcagc
     6481 cggcgatgaa ccacctcttc atccgttact agtccttcag gaaaataagg aaataagctg
     6541 cgaatcgttt cggaaatcgt ctcaggatta tccagttctt ggataaaaaa acgaccgagt
     6601 aattgattga tttgatccat gaaatgcatt gtttcctcat aagtatcttc tatcatccac
     6661 tcgttattgc tgttatacgt aattttttgc ctttttttct tctcaatatg cgaaaaagct
     6721 tcttttagct gcttgacttc atcttctagc agtagcgggg agactaaaat atattttcca
     6781 ccgtatcctg gtaaaataga tgtagaaata acaacatcat attcttcttg taaatcgatt
     6841 ttttttaaat cagcaatccg atagaaatga atttgttgga tgaagggaaa ataccttttc
     6901 aatcgtagtt ccaacatgct agtagaagct aatccacttg gcgaaaatcc tgcaatactg
     6961 atattgcttt ctttcggact tcgttccaat gaattcgcaa aatgaaggac catataagcg
     7021 atctcttctt cggataattt tttttcttga aaaatccgct ctactgcttt acggattgca
     7081 ttcgcaactt cctcgtattg aaccatgatt ttttctaaaa tcggattggt cagtgtctct
     7141 tcttgtaaaa cggctcttga aaacacacca gataaatgag tcaacagcat tttgtacaaa
     7201 gtttcatctt cataaaaatg gacattcgtc tcttcactta cttcttgaat cagttgtttt
     7261 actaaataag caagctgtgt atcgaacgtc tcttcaaaga aattctgatc aaatgagttc
     7321 gtgaagtcat ttaataatcc tgcaaaaaag acgatctcat ttactggata aagttgtttt
     7381 gtctcagaag cgatccgtga aaacaatcgt ttagctgtct gtagtaattc tttgtttgcc
     7441 agactattat atgtctcatt tgtcagtaaa tgatttttag tcacacgctc catcgtcaat
     7501 gtcagcatca aaaccaaatg ttcaagcttt ctatcactta gattggtctt tttttggatc
     7561 aattcttccc cgattacttt gcgtgcaaga gatagatgtt gcggatcaac gaatcgaaaa
     7621 aaataattca gtgatgcatc gaaagaaata ctatgaaaaa gttcatattc attgatttct
     7681 aattctaata cattcgctgt cagaagccgg cggtattttt ccggtccttc gatctcgtat
     7741 ccctggttcc gaatgatttc caaaggcagt tggcgaatac tttcttccaa ctgtttgata
     7801 tctgcataaa aagtggtatt actgatgcga tatgattcca tgaaatgagc aagactcaac
     7861 ggctctgtcg tcagtaacaa ctcggctaaa atcgcgtgtt gtcgtttagc agtggataag
     7921 tcattacttt tcccatctgt attttctttt aattgctcta atacttcttc tgatgcctca
     7981 atgacaaatg agcctctagc tgcttttttc aaagaagcat ttaccgattt caatgagact
     8041 tcaagattat ctaattcgcg gtaaatagtc cgtttactga ccttcaataa attcatcatt
     8101 ttttggatag atacaggtga cgggctactg agaaattctg ctaataattg tttttctctg
     8161 ggcgataaat acatcttata tttctccctt cacgagtttt tcgtattcat tcgtagctac
     8221 ccaatgttca actagaacta gccgagggat cttttcttct agctgttcag ttacagctag
     8281 ttctctttgt gcgatataaa tcgtttcttc ttggattttg attttttcta ttgattcttt
     8341 tgagataaca aactgttttt tcgctttttt tgctaattga cttaaaatcg tcatgcccat
     8401 agtttgtgag ccgcgtctgt tgtttttata caaaaataca atattagcag gcgacttttt
     8461 ttgttctgtg atatctgcta tctctttttt ttcttcccgc tttggaaatt ggatcgcttc
     8521 ttttattcgt gacagcaaga tatctgtttc caaaaagtga tccagtacat agatttgact
     8581 ttgaggtact ttttctcttg caagacccgc tagttcatgt tgcacgataa ccagctgatt
     8641 tacttggtta ttcaattcat agatagagct gtatccgaca gaaagatcga ttttttctgc
     8701 tgctaatttc tttttcagca gggcggctcc catagcactt gatcccatac ctgagtcgca
     8761 agcaaaaatg acggaatgaa tgatagatgg ttccaagatt tctccccttt ctttgaccgt
     8821 tttgttctct ttttctttat ttggtaattt caatagtatc gctgcacaaa tgaaggtcac
     8881 aaatgtactg attgctatcc ctatacttac cccagctagt tgtggataag gcgcattggc
     8941 aaaaatcacg agtagtgacc caggcgatac aggtgcagct aatcccgtat ctaaccattg
     9001 aaatatcaac gtgccgcttg ccccaccagc cattacagct aaaaatagat acggtcgcag
     9061 caagacaaat ggaaaataga tttcgtgaat cccgccaatc gcatggatca tcaatgctcc
     9121 actcgcattt actttttcct gtttttctgc aaaacaaagg atagaaagta agatccctaa
     9181 accaggtcca ggatttgcct ccatcaaaaa taaggaagag tacccgactg tttgtgagaa
     9241 ttcgattcct aaaggtgtca atacaccatg attgattgca ttattgaaaa atagtacttt
     9301 tagaggttct aagatcagac tgacaagtgg taacagattc attgtgacta gccaagacac
     9361 aacggaggcg agcaacaagt gaacactatt taaaactggt tcaataaaaa cgattccaat
     9421 tatacaaata acgctaccag tcagtcctac tagtaggttg ctgacaagca tttcatatcc
     9481 agccttgaat ttcacgtgga atctagaaac aaattgttca tatacgatgc cggcaagcgg
     9541 cccgataatc atcgctccta cgatttgtgg agcattggaa cgagcgatca accctaaaga
     9601 agcaattgca ccggcgactg cccctttttg tccccctacc gatttcccgc ctgaataact
     9661 gatcaagatg ggtaaaagca gctggatcat ccaatggcat acctcttcta atgtggtgtg
     9721 caaatcgcca gacacacctt ttgttgctaa tgacaagatt ccccaagcga taaacagact
     9781 tagatttggc atgatcattt catttaagtg atgtccagct tttctcagtt gtttcataga
     9841 cacgcgactg ctgttgtttg ctttccattt gtattccatc tttttcacca tacttctatt
     9901 tattgatgga cggattttat gtagaaaaag cagcaaacga aaaaccgtct gctgctttca
     9961 gctatctggg aactatttgc ctggttcagt cgcaatgatt gctagttcac cttggaccgc
    10021 ccactcagaa acgccatttg gaataataca gcttgttcct ttttctaatg gatatgtttt
    10081 tccgtcaacg acaaggtctc ctgctccttc gattaccgtc atcaatgtat aaggggcttg
    10141 ttttttgaaa gatgtgattc ctttaatatc ccactcatat acattgaaaa actcagtttc
    10201 taaataagtg acgatcgatg aattcccttt tcggacttct ttgatttgta attctggtgt
    10261 tttcgcaggg acagttgtta catcgatcga ttgttggata tgcagctctc ttgtttgtcc
    10321 ttgcgcatct tttctgtcgt agtcatacac gcggtaagtc gtgtcagaac tttgctgtgt
    10381 ttctaaaatc atgattcctt taccaatagc atggatcgtt ccgcttggta cataaaagaa
    10441 atcacctttt ttaactggta ctttcttcaa aaggtcatcc cagcgcccat ctttgatcat
    10501 ctcagctagt tcttctcttg tctttgcatg atgaccatag atgatttctg caccaggttc
    10561 agcatcgatg atataccagc attcagtttt tcctaattca ccttcatgtt tcatcccata
    10621 ggcatcatct ggatgtactt gtacagatag atcatcttct gcgtctaaga tcttgatcaa
    10681 taatggaaac actggttctg aaggatggcc aaacaattcc ttgtgttctg accatagttc
    10741 atctaatctt ttccctttga attcgccatt ttctactaca ctgacaccat gaggatgcgc
    10801 actgatcgcc caatcttctc cgattttatc gttagggata tcaaaaccaa agactgaacg
    10861 taaacgactt cctccccaaa ttttttcttg aaaaactggt tttaaaaaca taggttcctg
    10921 cataattcaa actcctctca tttttcatag ctattatagc attatttcgg taataccgct
    10981 ttctctttca aaaagttttc aacaatttca tctcgacata tgagatactc cgcacgatga
    11041 tcgacaggat gtacgcgatt tgacaaaaat atcaacgctt cttgtgtttt tatatcaatc
    11101 aagaaaaacg tacctgtata accagtatga aaaagaatcg catgaccgtc ttctacatcc
    11161 ttttttaagt cccatcctaa agaacgatag ccattttttt tcggtgtttg atcttgcagt
    11221 aaagaatcaa tcgtctcttc ttctaaaaaa cgaccattac ggatttttcc cttgttcaaa
    11281 tacatttgga caaaagtaat gctgtctttc aaatcaataa agagacccgc gttacctgca
    11341 tgttcagcca aaactcttgc tttcggatca tgagtttgtc cacgcaaaac ctcacccgaa
    11401 ggcagttgtt ctgtgggaac gatttttttg gaaggaaaag gaagaaataa gctgttcatc
    11461 attcctaatg gttccaatac ttgttcttga aagatttcca ctgcagtttc ttggtaaatt
    11521 tcctctagca tcaacccaag aagaatcgta ccggcatctg tatattttac ttcttttcct
    11581 agttgctcac ctggttctag cttcaaataa gcggcgatca attcttcctg agatagctta
    11641 tctctgtttg gtatccatgt tttgatatca gaagtatgtg tcagtaaatg acgcaaagtg
    11701 attgtctgat tttcaaattc aggaagatat ttatgaagcg gttgatcgag ttctatcttt
    11761 ccatcttcta atagacgtaa aactaatgaa gtggttgcaa cgactttcgt cagtgaggct
    11821 gcatcaaaca acagatcttc tgtcatcagc tgcttttcag gaacgacttg ggcaaagccg
    11881 gctgtaaatg tttctacttg attttctttt atgaaggcga ctgctacacc tggataaaat
    11941 ccttcttcca tctttttcaa aataagctgt ttcgtgtgtg gatacatctt tttctcctgc
    12001 ttttctatca gattcttaca gtccaagctt aaagcttaat ctatgaggaa tcaacctttt
    12061 ttacacaaaa aaactgcgcg aaaaaccgcg cagtttcccc cggtatacta ttttctcttt
    12121 ctatctctag aataagcgat agtatgaccg ttcataaaat gacgagacat gcgacaatcg
    12181 ttaagaaaga aaaagttaag cgtcgcatcg aatgtcagtt ttattctttt cttttggctc
    12241 actgacgaac caccattcga ttcctatagc atcatagatt gtgagtactg tgtgcttttt
    12301 gtctaagaag aaatcaactg atagttgctg caagtgagca atcagtcctt ccatgctttc
    12361 agtatcgatc atgaatttca gaaaatcaag tccaatcact tcatctgtag gcaaatcaat
    12421 tgcgcctccg ctagcttctg tcaaaccaac atggaaatct ccttcattca atacatggat
    12481 gccctcttct tcattatgta ccttcaaacc aagtacatga ttaagaaatt ggcgttcttt
    12541 tgttaagtta gtaacattta aatggatttt ttcaaaataa gaacctgttg atagtttact
    12601 gaatttttct tcagattttc ttaataatgc ttcttgatcc attgtttctg gaatttctct
    12661 ttggtcgttc ttattctctt catggtaaat ttccaattca ttctcttctg ggtctacgag
    12721 caaaatcccc atacgtccat gatcttctaa agcttctttg attggataat tcattttacg
    12781 gatacgacaa aggatatctg ccatttcttc tgtgctaggg ataatcaatg aaaaacggtt
    12841 cagctttttg atcaccccca tgtgatcatt cgctctagga ctttcttcca gcagcaacat
    12901 ttcacttcca gcttcttctg ttcctaaaat ggctaattcg ttttcttctc ttttcaaatc
    12961 aaagccaaca acgttcttgt aaaaatcaat cattttatct cggtctttca cccgaatggc
    13021 tacacttttt aaataggtgg cagcccccaa ttgaaaatct ttcatatttc ttgctcccct
    13081 ctacgtcaag ctttatttta acgatggatt cttcttcttt caagcaataa ctttgatttt
    13141 gttaaaaaaa atgtgagaaa attcgcattt tgcttgtttt ttgtaaatta atgagtaaaa
    13201 actcttataa aaagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
    13261 caaaaatagc tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
    13321 gaacaccgtt taaggaagag gttgtaaaat cagcggtttg acctgagtat tagagataca
    13381 aaacggaaaa atagcttctc atattttgcc gttttttgag ctaataccga aggacagctg
    13441 attgaacacc gtttattcgg aaaaagggac tgtgacacga cttttgtcac agcccctaaa
    13501 aaaaataata aatttgtact attctatgaa gcttcttctt cagaaaactg actgttataa
    13561 agatctgcat agaatccgtc tttttccatc aatgtatcgt ggtttcctgt ttctacgatc
    13621 gaaccatgat tcatcacaat aatgttgtct gcatcgcgta ttgtagacaa gcggtgagca
    13681 acaacgaaac ttgtccggtt ttccaatagg cggttcatcg ctttttgaat cagtatttct
    13741 gttcgtgtat ctacactaga tgttgcttca tcaaggatca ggacatctgg atttgctaag
    13801 aatgcccgcg caattgtgat tagctgacgc tgcccttgcg aaatattgct tgcatcctcg
    13861 tttaatacag tgttatatcc ttctggcaat ttacgaacaa aatcgtctac gtgagcagct
    13921 ttagcagcct caatgatttg ttcgtctgtt gcatcgtcgt tcccatattt gatattgtca
    13981 tagatacttc ctgtaaacag ccaagtatct tgcagtacca ttgaaaactg tgcgcgcaat
    14041 tcatcacgtg aaagatcacg ggtatctaca ccatcgtaac gaatactacc tccagaaaca
    14101 tcgtagaacc gttcaagaag gttgatcagt gtcgtcttcc ctgcaccagt cggacctaca
    14161 atagcgacca tctcaccagg tttgacatcc aagttaaaat cagtcattag caagttatct
    14221 ggtgtgtagc cgaattgaac attttcaaaa cgaactttgt atggtgtatc tttttcttct
    14281 ggaaggccag atttttcatt ggacatctct tcttcatcca acacttcaaa tacccgttcg
    14341 gctgaagcaa ctgttgattg gatagtgttc atcaaacttg caagttgagt gattggttga
    14401 gagaactgtg ttgtatattg caagaacgct tgaacatccc ctaatgtcat gttcccattt
    14461 gcaactttta ctccgcctag tacagcgacg aacacatagc ctagattttt aacgaaattc
    14521 atcaacggca tgatgattgc cgaaataaat tgagctttcc aaccagcggc atacagattc
    14581 tgattttctt tttcaaatac ttcctggtct ttttcagtat gattgaatgt tttaacaatg
    14641 gtatgaccac catatgtttc ttctacttgg ttattcaata aacctaaact tttttgctga
    14701 tctgcgaaaa acacttgtga tttcggcgct acgatcatca cgatgatcaa gcttaaaggg
    14761 atcatcaata acgcgactaa cgtcaattgc cagctgattg aaagcatcat cacgatgacc
    14821 ccaatcaatg tgactgcact agttacaaat tgagtcaaat tttgctgcag cgtgcttgcg
    14881 atgttatcca tatcattgat tgctcgagac atgatatccc catttgtatg tgtatcataa
    14941 taggaaatcg gtacacgatt cattttggct tccaaatcat gacgtaattc ataaactgtt
    15001 cgttgggaaa cacgtgtcat aataaattgc tgtaaaaagt tgaatacagc agaaatcaaa
    15061 tacatcacga ttactgtcgc aatgatctgc gcgattttat caaagtctat cggaaatttt
    15121 gttacttgtt ggccttgttg catctgttga gcgccagcca ttacaccttt gaatatttct
    15181 gtggttgctt tccccaagac ctttggtgtt tggatttgga agatcacggc tgctattgct
    15241 agtacaaata cagcgatgat cgcaaccata cgtttagaca tatagccaaa caatcggcgt
    15301 accgttcccc agaaattttt aggcttttca ccttttacac cgagatttct gccaggtcca
    15361 ccatgtcctg gaccgcctgc tggtccatga gagggacgtg tgtttgtttt actcatgcta
    15421 agtcctcctc tcttagttgt gaatgaacga tttcttgata agtttcgttt gttgctaata
    15481 gttcttcatg tgttcctttg ccaaccagtt ttccttcatc tagcacaagg atcatatctg
    15541 catccattac tgtacttact cgttgagcaa ctaaaacggt gatactttct ttcatattcg
    15601 ttttcaatgc ttggcgaaga ttggcatccg ttttgaaatc caacgctgag aaggaatcat
    15661 caaacacata gatatctgct gttttgacta atgcccgtgc aattgccaga cgctgtcttt
    15721 gtccaccaga gaagtttcct cctccttgtt ccacatggct atctaagcca tcttccatct
    15781 ctgaaacaaa atctttagct tgtgcgattt ctaatgcttt ccaaatcatt tcatctgttg
    15841 catccggctt accatactgc atattgtctc ggatcgttcc agagaacaga actgcttttt
    15901 gaggaacaaa gcccattaat tctcgcaaat tatattgagt catgtcacta atgtccgttc
    15961 catttatttg gatcgaacca gattcgatgt catataatcg agggatcaaa ttgactaagg
    16021 tcgttttacc tgaaccagtc ccccctatga ttgcgacgat ctcgcccgac ttggcttgga
    16081 aatcaatatc ttccaatgca agtttttctg ctccatgata tcgataattg acatgattga
    16141 aggccaatgt tgcgtttttt ccagcaaaag aaactgtttt aggattttca gggtcatgga
    16201 taccgatttt ttcatccaac acttcattaa tacggtcagc tgaagcttgt gcacgaggca
    16261 ccatgataaa aatagcggaa agcatcataa agctgatcaa gatctgcatc gcataagtca
    16321 taaatgcaat cagattaccg acttccaaag tcatatctgc tatgtagtgt ccaccaaacc
    16381 aagtgattgc tacgtttgta ccgctcatga tcaatgtcat cattggcatc atcaatgcga
    16441 cgatcgtatt aactttgatt gccgtatttg tataatcttt attcgctaaa tcaaaacgat
    16501 tttcttcaaa gcgtgtctta tcgaatgctc gaatcactct gacccctgtc aatccttctc
    16561 gaaataccag gtttaaacgg tcagttttct tttgcatact tttgaataat ggaacagcaa
    16621 agtacatgat tccgccgatc aaaataatca ttactgggat gacaactaag aagatttttg
    16681 tcaactgatg atctttttga taagcaagta tgctcgcgcc aatcaacgtg attggtgcat
    16741 tgatcatcat acgtaaaaac atttgtgtaa ccatttggat ttgagtcaca tcattggtcg
    16801 ttcttgtgat caaagaggcc gttccatatt tatcaaacgc atcttttgtc agactctctg
    16861 attttttata gacatccgta cgcaattgct tacccagttt ctgtgattca cgagtagcaa
    16921 aaaatgtatt tgcgatagca gctaagatac tgatcaaaga aaagccgatc atcacaaaac
    16981 ctgtatgcca aatataatcg acatctcctt gcgctacccc tttatcaata atattggagg
    17041 taagtgtcgg caaatataaa tctgccagta cttggatcac catgaaaact gcagctgcaa
    17101 ttgctgaagt caaggagatc cgttttacga tttttagcat ctagttccct tctttctatt
    17161 tatatactcc gtttttcagc caagaaagtt tcaaattgaa ttcactgatt gcttgctcta
    17221 ctgagtaatc ttcagatgtt gaaaataagc gatacgaatg cgcaattgtt gtaaatacag
    17281 tattcatcac taattggatc agtaattcca attcatgatc attttttact ttcagcttcg
    17341 tccgatcgat caattcaagc aactcttttg catcttgttt gtgttcttta tgtggatcgt
    17401 gaggcgtttt ttggcgactc gtatgaggag cgacttttct tgaggcacgg taatccatgt
    17461 tcataaacaa gttacgataa aaagaagcat tctcgccttc caaaatctca taaatcattt
    17521 tagaaaaata ggattcgaat cctttgaata gatcaccatc ggattctttc attagttgta
    17581 acatatcttt agagctgtca cgtctcattg tctcaaagta ataaaaataa agatcctctt
    17641 tatcttcaaa atactgatag aaactgcctc tagggatatc cgctaatttg acgatattag
    17701 caatcgaagc ttcttttaag ggtactctag aaaactcgat tctcgcagcc tcgattagtc
    17761 ttttttgttt ttctttaggc aaatggaaaa atgtctgtgt cggcatatgg atcctccttt
    17821 ctttcaagat gacacattgt catatgacgc tgtgtcatta taacacctcc tacctgtttt
    17881 gcaagacatt tgcatagttt ttttcattat catccgatac attaaaaaag tgataagagg
    17941 aatccttcca tttgaattaa tgagagttgc ccattaaaat agccacatac taaacaaagg
    18001 atgagataaa tggcccaatc aattagtgtc caagacttca ataaattgcc tctcgatgct
    18061 gacagcatcg ttttagatac aagaaatacc ttagattaca caaaaaacca cttaagccac
    18121 tcgctttcta tccccgctgc tatcttgcca agacaattaa aaaatctaga tccaaagaaa
    18181 ctttattata ttctaagcta tacaggcagg cgatcagaag cattagccga tcaacttact
    18241 gctaatggtt ttcgtgctgt ttatatcatt ggtgggatgg aagctcttca gtcgagtgtt
    18301 gcttgaatat tgcttgttag attcaaaaga ggctgtgaca aaaaatcgtg tcacagcctc
    18361 ttttataccc tgtctttttt tagttattta ttcttcaacc ttatcaatat gacttgatag
    18421 ccttttcttt ttctcatagt tataacaatc atagcaataa aaggagaagg taagcatatg
    18481 gcatctgttt tagtagtcaa aggtcatccc ctaactgcag aagaatcgcg cacagttaag
    18541 gcattgacgt cttttttaac aagttataaa gaaaatcatc cagatgatga agtgactgtt
    18601 ttggaacttt atcgcgatga tattccggaa atcgatgaag aattattatc aggttgggaa
    18661 gcattacgtg ctggtgcaga attcacttcc ttatcagaaa gtcaacaaca aaaaattgct
    18721 cgtttcaatg aattgactga tcagttcctt gctgctgata aaatcgttat cgcaaacgcc
    18781 ttatggaatc taaatatccc aacaaaatta aaagcttggt tcgataccgt caatgtagct
    18841 ggaaaaacgt tccgctatac agaaaacggg ccagaaggat tagtgacagg taaaaaagca
    18901 ctgcacatcc aatctaatgg cggtgtatac aacggacaag actttgcctc tcaatatgta
    18961 aaaggtattt taaactttgt aggcatcgat caagtagatc agctattcat cgaaggaatc
    19021 gattatgacc ctgaccgtgc agatgagttg atgcaaaatg cattagataa agctgctgct
    19081 ttaggtaaat cattttaaaa attgagataa atgcatacaa aagagacaga ggaaaaaatc
    19141 ttcaaagcag catttgtacc ttggatattt tcccatctgt ctctttttgt tattctttct
    19201 gctttttcaa cggattttct tcataaagag aagagattgt tgtgtaccat tcaaaaacat
    19261 gagtaatcac tactttagct gctgcataaa caggaatccc caagatcaca ccgatgattc
    19321 cgaacaattt cccagaagtc agcagaacta ataaaatcgt tactggatgg attgctaact
    19381 gattccctag tactaatgga gagatcaagc gtccttctat tgtctgctct actgcaaaaa
    19441 cgatcagtac tttgatgatc atgaatggac ctgctacaat ggctaaaaag atagccggaa
    19501 tcatcgctaa aaatgatcct aagtaaggga tcaggttcaa aaaacccgca ataatcccca
    19561 acgtgacagc atactctaaa ccaatgaccg caaaaccaat cataaacatg attgccacag
    19621 caaaagccac agttagttgt cctcttatat aagaagaaac ttgactattc atttcactca
    19681 gcacattcaa cgtcggtttt tgcatgcgtg tcggtaaaaa tttgacaaaa taaggtgcta
    19741 attgtttacc gtctttcaat agataaaata agataaacgg catcgtcagc agagccacta
    19801 aaatagtcgc taccgcgccg acaaaacttc caagactctg aactgtactc ttagatatat
    19861 cttggatcat atcgcctgcc gaattcatga acttatcacc tgctgtttcc aactgttcgc
    19921 gaaattggtt gaacagcggg tcccgcaaaa tctcggttaa tttattatca atcgtatcta
    19981 catattgagg gaagttactg atgaagctca ctgtttgttc ctgaatctta gggatgatca
    20041 ctacgctccc ccatgcgatt agtgcaacaa ccaaaacaaa cagcccaatg atactgtaaa
    20101 gccttggtac ttttcgcttt tcaaagtaat caactaccgg attcatcaag taatacaaga
    20161 ttcctgctaa aatgataggc agacctacga ttgctaaaaa ttgccagatt ggtgcaaaca
    20221 aatatgaaac ttttgtaaat aaaaagacga tcagcaatat taacaataca acaagtaagg
    20281 ctgtgactac ttgattattt aaaaaccagc gccaaaacca cgataccggt ctgttcttct
    20341 tattttttcc ttccataatt cctcctgtta gttataaaaa atttggcttc ccaaaccaat
    20401 agcaggcagt ctatgcggat gaagatagat tccatttgca atttgtcctt cttctggaca
    20461 agcatcaaaa atcaatgata cgtgagctac tgtctctaaa ttcccattgg ccatcggacc
    20521 gacataatcg atcgtatata cctgatcatc aaaagcaatt gttccgcctg ctttcaaaga
    20581 aaaaggttcg tcttttgata cttcttgaat caccgaatat tttcgtaagg cagctgttgc
    20641 tgttttatcg aacaaaatca gcatgggttc ttccttgttt attgcttctg tcccaatttc
    20701 tgtaatcttt gcctgaatca ttgcactctc tcctttaatt gctttcaaaa acagtataac
    20761 acattctcat tctttagccg tgtggaataa ttatgtgata gaataaaaac gaaaactatt
    20821 tcattacaat taggaggaat cagctcatat gatcgaaaag ataattttag gaacctatac
    20881 gcgtcgcgaa agtaaaggaa tctatacgat cgaccttgat acagaaaaag aagaattagc
    20941 caacctttca ttagctgttg aagaaaatag ccctacctat gtagcaaaaa gtaaagctgg
    21001 caatctttac acagttacta gtgtggatgg attaggtggt gccggtgcgt acgataagga
    21061 ttttcatttt ttgaatgcgg taacagagtc tggcgctcct ttgtgttatg tagcggttga
    21121 tgaagcacgt caattactat acggggcgaa ctatcataaa ggtgaagtaa atgtctacca
    21181 tatattagga gatggcgaac tgaaagcagc agattcaata ttccatcaag aagcgacagg
    21241 tcctcataaa aaccaagatc atgcgcatgt ccactatacg gatctgactc ctgaccaacg
    21301 attagtcgta tgtgatcttg gtacagatcg tgtgtatact tatgatgtgt ccgaaaacgg
    21361 caaattgaat cttgtaacta ccttcactgc agagccagga acaggccctc gccatcttgt
    21421 ctttcatcca aatggacaat tcgcttattt atttggtgaa ttagacagca cagtccgcgt
    21481 gctttcttat aataaagaaa caggtgcttt tgaagaatta cagaaaatat cgaccttgcc
    21541 agaagaattc gacggtgaaa atggaggggc agctatccgc atttcaaatg atggcaagtt
    21601 cctttatgct tctaaccgag gacataactc gattgctgta ttcgcagttt ttgaagatgg
    21661 ttcccacatt caaaacaagc aaatcatctc aacagaaggg gattttcccc gcgatttcgc
    21721 actagatcca agcaataatt ttgtcgtgtg tgccaatcag aacaccgata atctgacatt
    21781 atatcgccgt catcctgaaa caggattact ggaattactg aaaaaagatg tctatgtacc
    21841 agaatgtgtc tgtgtttatt ttgatcgtta gtaagaaaag gagaatgaaa tgaatcagca
    21901 agacgtatta gacagactac gggaagaact aaatattcct ttctttgatg ggaaaatcga
    21961 agataaagag tatagcgaag aggaatatca gaaactcaaa gccgatttac agaactactt
    22021 tgaacaatat gtacagaatg tagaaaacta atcagctttc catactatct gaaaagaaca
    22081 tcaggacaag acttttccgt ctatcctgat gttcttttct acaaataaaa aaccgccgag
    22141 atggttagac tcgacggaaa aggagtattt actcgatttt gagtaagtac aaggttgttg
    22201 ttggttatag gtatgtcatt atcataaagg attttgatca ctctatcata aaggtaatat
    22261 tacatttata ttagataact ctagttttta ttgttatcaa agatttcttt tattttttca
    22321 acaacaaatt agtcattttt tggatggcag ctgtcaattg gtctgtcgct tggtcttgtt
    22381 ttgcttcaga tgtattgaat agatcttcat ctacccctga ttcaagatcg atactttgga
    22441 tcatttcttc acagcctgct acatatgaaa gataagcttt ttcaaaattt ttatgaatgc
    22501 ccattgcttt tgctggtgga cgcaaagtac tgactttctt aagcattgcc cgatattttt
    22561 ttgttccttc ttcaaataac aggatgatct cttcaagagt cgatttgtcc agttcacttg
    22621 tttttccttc atcaattgcg tttctgatca cttcatattt cggatgcatt ccttctccga
    22681 ttgcttctgt ttccttgacg atttgatcga tggactgcac ataatagcca atattcggta
    22741 acatgttcgt tttcctactt tctccattgt tgttattatt tattctactt gtttttatcc
    22801 tcaaatgaca agtacttgaa aaaatttaat ggtttctata cagctcgcca ttcttattcg
    22861 tgctcaaaga aaccatctgc taaactatat tgtttccctg cgacaatgtc atattggatc
    22921 aattgatatt cgtcgtaaat ctcttgttgc gagcgattga attgcgcttc tttataccac
    22981 tgtccgtttt gatagtacag ttctcgttca aacgccggaa gttcctgttc taatgataaa
    23041 agcagctgat aaaagccatt tgttgtctga ttcgtctgtt ccattagatt cgctgcaaaa
    23101 taaaacggac tggtgattgc atcgtgagtc gtttgctttt ctaattctcc tttactgtcg
    23161 aacataagaa attgtgtttc atgcaatgtt tgtttgtcat tttttgcttg gatcgtatct
    23221 gaatagatcc ctggaagatg atccccccaa aaaacaacta aagtccttct agaaagattc
    23281 ttcaattctt ccgtgaactg cttcagtgcc gtgctgctat aggaaatatc ttgcagataa
    23341 ttctctaagt caagtgtccc acttccatct gaagtttttg caggatagtc tagattatca
    23401 tactttccat cgtagggcat atgtgtctgc atcgtgacta aatgaatgaa ctgaggtgtt
    23461 ttttcttcct tgagaagtgt cattacttct ttgtaagctg actcatcaga aatatatgga
    23521 ttattctcta tcgtatccgt ataggtcatc gtacgctcgc tgatgaactg gtcaaatcct
    23581 aatgtctgat aaacatcctc tcttttatac atcgaagtat tgtatggatg gatcgctgtc
    23641 gtctggtatg attgtgcgtt taacgctgaa acgatagaag gaagctgatc catttttggt
    23701 acaagcatcg tgtaaggggt ggtgagttgc gcattaaata acgccataga aaaaccagtc
    23761 aatgcttcaa attcgatatt tgctgttcca ccgccatagt tttgagaaag catattgcca
    23821 ctatatgttt gatcagctac ttcataataa tctgccaatg gctcaccaga tacttctacg
    23881 ccgtttaatc gactaggatc tgagaaactt tcactcatca cgaacacgat gttcggcgac
    23941 tcttcctcta cagttttatt tttttcatcg gctagcttct ggtatttttc agtgatttct
    24001 ttgatctttt ctttcgaata tccttcaggt tcatccattg gttctacctt cagattgtac
    24061 aaaaacccac cgataaaacc agtgttatag tagttcattt tctggctgta aggtatccat
    24121 agtgccgttt tattatatgc ttttcgcaac aaattatcgg gattgttgaa attactgata
    24181 taccctagca gtccaattgc cacaagcagt gtcgttaatc ggatcaattg gaatcgcctt
    24241 ccttttttca ggcttcgaaa catgtaccag caaaacaatc ctatcacggt tcctgcggca
    24301 gctaagatca ctgtaaacag catagtgccg gtcatatctt tcagcaatcc gatttcagtg
    24361 atcatcttca aatcgtctgg ataaatcggt tcttgccgat aactcatttt catataattt
    24421 gcaaaaccta atacaccgat ggctacgaca tacgtaaggg aacctaaagg gacagatcct
    24481 gctagtgcaa tcagaaaaat aagaataatc agcaagacga gacaggctaa gaagaatttt
    24541 tccgtatgcc aagaaaaagc aaatttcaac gcaaggtcta cggatagatc attttgacac
    24601 cactgcaaaa ataaattact aaaaacaaca agaaaaaaga tactgatgat tgctgctgtt
    24661 ccttttaaaa tctttttctg tttggtcgaa agtctgctta tatatctttt caaatgcttg
    24721 ctccttcatt tctatcaata aaagaatgtt gcttctctta ttttagttgt acgaaattcg
    24781 aaagtaaaga acggaaagaa gaattaatga aatccaaata ttctctttct ttatggcctt
    24841 tctggtttct ttaataagcg gacctttcgt actccagaaa ttttttctag ctgttgtttc
    24901 acttgattga gctgttcaaa atcagtactg tctatgtcaa tcaatatgta gacatgctca
    24961 ttcttatggt tacgcgatat atctgcgatc gctagctcgt tctcattcag cagagaaaat
    25021 atctctgcta atatattggt tttcttttga taaaatatag tgaatcgaaa cggcgcttgg
    25081 aaaaacattc gtgcggatgg ataattgacc gcttctctaa ccgtaccgaa caaaagataa
    25141 tcttttaagg cttcaactgc caaacgtccg ctgtctgcca aagcttcctg agtcgatccc
    25201 cctagatgag gcaacataag tatacggtca ttttctaaga actcttcttc tggaaaatca
    25261 gtcaagtatt tagccagatg ttgtttttcc aatgcttgca taacagcata tttatcaaca
    25321 atttcacttc ttcctacatt gataagtaca gcatcttttt tcattttttc tatattcgcc
    25381 tgatcgatca atccttttgt atcttctgtc aacggcagca tgactacgat gtaatccgag
    25441 gtagaaagaa ctgtttctaa atctgcttgc cgaacataat ccaactgggc atcgcggata
    25501 gaataaccca agacatccat ccctaagcta tagcaactta gcgctacttt tgtgcctatc
    25561 gctccaagac caagaagacc gatggttttg ccttgcaatt ctcgtcccac ataagcactt
    25621 cttttgtttt ctgcctgttc taaaatatta ggacctgtta gtgtttgtac catacgactt
    25681 gcttcgatta tcggacggga agataagagc aaacagcaca aaacaagctc ttttactgca
    25741 ttagcattta cacctggtgt attcatgacg atcgtcccgt tttcagaagc tttttcaacg
    25801 ttgatcgtgt tcaccccaac accagctctt gaaattgcta gcaaatctgg tgtaagccat
    25861 tcatctttca ctttcgtact acgaataact attccttcgg ctggcaaatc atctattatt
    25921 ttgaaatatt ccccttgtaa cttttctatc tcttgttctg gaatagcatc aatcaaatga
    25981 acgtgataca taaaaccccc tccttactaa caagtatctc gaaatttctg caattagaca
    26041 actgtttacc atttgacttc ctagaagcaa aaagagcagt tagagcagag taggctgagt
    26101 gataaaaata aggctatgac aagttactgc cataagcctt attttcgaga gcaaaatatt
    26161 gagggtgaga tatcttgata aatggtgttc cgttagctga cattgataat aagactaaat
    26221 ttcaaaaaag ttgaaaagca aatttcgtca atctcttctt atttcttaaa actaagtggc
    26281 tttttcacaa cctcttttta tctaataacc actgtttcca actcaactaa ttttgcccac
    26341 gtttcaattt gatctgttgt tagatttagc gagacaacgg tatgatgacc gccacctgcc
    26401 tgaatccatg aatggacacc ttcatggaaa ttcggtttag ttttccataa cacacgtgca
    26461 acaggtaaat taggtgcttc agtcgttggt tcaaaggctt ctacttcatt gatcagcagt
    26521 ttataatgcg taccgaaatc tgccatcgat acgactaccc cttctcctgc tttcccatca
    26581 aatacaagac gcgccggatc ttctctatct cccattgata aaggagaaac aacgattctt
    26641 ggtttcgtcg cagcaagcgt cggatctact tccatcatat gggattccaa gattgcttct
    26701 tggccactag ctagctcgta agtataatct tccatgaatc ctgtatcttt ccctcgagcc
    26761 atgattttca gcaaccgatc aatcgctgca gttttccagt cgccttcccc agcaaaaccg
    26821 tatccttctg ccatcaaacg ttgtacagct agtccaggta gttgtttcat tccatacagg
    26881 tcttcaaaat tagtcgtaaa agcgttatag cctccttctt ctaagaaacg gcgaataccg
    26941 atttcctgct gagcttgtac ttttacatgt tcctcccatg tcttttcttc ataatcacca
    27001 tagtcgaagg tatacaattc cttgtactct tcaaataatt gatcgacttc ttcgtctgat
    27061 acgcgatcga ttacttgtac aagatctccg ataccaaaat aatcaacagt ccaaccaaat
    27121 tgaatctgtg cttccacttt gtctccttct gtgacaccca cgtttctcat gttgtcacca
    27181 aaacgagcca cttttattcc aaaactttca ttataggcta ctgcaacatc catccaatca
    27241 gcaatctctt tttggatctc aggtcgtttc cagtagccaa cgacgatttt attttgttta
    27301 tttaatcgag cattgatgaa cccatattcg cggtcaccat gagcggattg atttaaattc
    27361 atgaaatcca tatcgatcgt tttccaagga atgctttcgt tatattgcgt agctaaatga
    27421 agcaatggtt tttgtagaag ctttgtcccg cggatccaca tttttgctgg tgagaaagta
    27481 tgcatccaag tgatcacacc tgctacttct tctcgataat tgacctcttt catgattttt
    27541 gtgatcgtat cagcagtaac tgctaattct tgaaggcgga ttggataagg caattggcca
    27601 ttttcattca gttcatcgac catttcttgt gcatgtttct ttacttctcg cagtgcttct
    27661 tccccatata aatgctgtga tcctacaaca aaccaaaatt ctttttctcc aatattcaac
    27721 ataattagcc actcctttta tttgttttgt ccgtaatacg cattttttcc gtgttttcgt
    27781 aaataatgtt tatctaaaat ccgttgaggc agctcttctg aaaatgaatt gagctgtctg
    27841 gtaaataaat tcatcttgca tacttcatct agtacgactg cgttcattac cgcactttgc
    27901 gcatctttcc cccaagtaaa tggaccgtgt ccgtgtaaaa gtacccctgg aattgctaag
    27961 ggatcaattt tgcgttcttt gaatgtttct atgataactt ttcctgtttc atattcgtac
    28021 ccgctgtcga tttcctgttg tgtcaaaaat cgagcacaag gtaccgatcc ataaaaagta
    28081 tctgcatgcg tcgtccccat cgcaggaaca tccaatcctg cttgtgccca gatcgtcgcc
    28141 catgtcgagt gagtatggac gattcctccg atttgaggaa atgaacgata taaaacagca
    28201 tgagtaggcg tatccgaaga gggattgagt ttaccttcaa tgatatgatt gtccaagtcc
    28261 acaacgacca tgtcgtctgc tgtcatctct tcataactga ctccgctagg tttgataacg
    28321 aaatatcgtg tttcaggatc aaaagcgctc acatttcccc atgtatattt gacgagtccg
    28381 tgtttaggca agtcaagatt tgcttggtat acttcttctt tcagttgttc cagcattttc
    28441 tatcactcct ttttcttgca attgatgaat cgctgttttt tcaatgtcga tcccctcacg
    28501 gtaacgtctc ataaatatct gatagcctgc tatttcttct gctgaaggtg tgatggtgat
    28561 tccttggtct tgtccaaaca cgtcttctgc caagaagttt tctaaagact tcccatgtcg
    28621 atccatcata taggctgcta ataacgcgat tccccacgct ccaccttctc cggctgtgtc
    28681 catcactgta actggtgcct ccatagcaga tgctagaatc cgctgtccaa cttctggtgt
    28741 tttaaaaatc cccccatgcc ccactaaacg atcaatttgg acattctcag atttcaatat
    28801 atccattccg attctcattg caccaaaagc actggctaaa tggatccgca tgaaattcgc
    28861 taaatcaaaa cggctacctg gtgtacgtac aaataatggc cttccttcat ttactcctgt
    28921 aatgttttca ccggaaaaat aaccataaga aagtaatccc ccgcaatccg tatcgccttc
    28981 tagcgctttg ttgaataata ccgtgaataa tttatctctg cttatctcca tacccaaaga
    29041 attggtaaat tcttcaaaaa tactgaccca agcattgata tccgaagagc agttattcgt
    29101 atgaaccatg cctactagtt ctcctgaagg tgtagtgacc atgtcgattt ccggatggac
    29161 attttctagt tctttttcta atacgatcat agcgaaggcg gaagtaccag ccgaaatgtt
    29221 ccctgtccgt ttggctacac tgttcgtagc gaccataccg gttcctgcat ccccttctgg
    29281 cggacaaacc gggattcctg gtctcaaatt tcccgtaggg tcgatcagat aagcccctga
    29341 ttcgcttaac ttgccagctt cttctcctgc tactagaact ttaggcaaaa tgtgatccaa
    29401 gtcaatcgct acgtgcgctt tttcgattaa ctgaccaaaa atatttttca tttcttgatg
    29461 gtaggttttc gttgaaggat cgataggaaa catgcctgat gcatctccta cgcctagtac
    29521 tttctcacca gtaagctgcc aatggacata acctgcaagc gttgtcaaaa aattaatttc
    29581 tttcacatgt tcttctttat tcaaaataga ttgatacaaa tgagcgatac tccatctctg
    29641 cggaatattg tattgaaatt tttccgttaa tattcgttct gcttcttcag tgattgtatt
    29701 tcgccaagta cgaaatggta ctagtaactg ttccttgcta tcaaatgcta gataaccgtg
    29761 catcattgca ctgaacccaa tcgacccgat tgtcgtaaga ttctcgccat agatttcgaa
    29821 tacttctgct gccatttttc ggtaactgac gcgcagtcct ttccaaatat cttctaatga
    29881 ataggtccag ataccatttt ctagtttgtt ttcccactca taactaccag cggcaatagg
    29941 agtaaaagat gaatcaatca atacagcttt gatcctagtt gatccaaact caatccctaa
    30001 agatgtacga ccatctatga catctaacat actttgcgca tttacttcct tcataatgat
    30061 ttaactccct ccttctcgtc aaacctgatc aaaccgtctt tttagtagcg cttacaatga
    30121 ttattgtata cttttgtacg tacatgtcaa gcggttattt acaagaatac aataaaaaac
    30181 acttgttttg tacgtacata agttataaaa tgaaattgct tacaaaatct gatcaaccgc
    30241 cttctcgaaa ttctagaagg agagaatact tatgaaaaaa gaaaaaaaag tttcctcatc
    30301 gtttatttat ttttttggcg catttggcgg cattctattt ggatatgata ttggtgttat
    30361 gaccggcgcc ttaccttttt tacagcatga ttggggtttg gcaggtaaag cttctttgat
    30421 aggatggatc acgtcttcgg tcatgttagg tgcgatcctt ggcggctctt tatctggtct
    30481 tctttctgat aagcttggac gacgaaaaat gattcttctt tctgcactta tttttatggc
    30541 tggttctgtc ctttctgcat ctgcccctca caatggttct tattttctga ttgcagcaag
    30601 gattcttcta ggtttagctg ttggcgctgc atctgcccta gttcctgctt atatgtctga
    30661 aatggctcct gctagactaa ggggaagatt gtcagggatc aatcaagtga tgattgcttc
    30721 tggcatgctc ctttcctatg ttgcagatta tttgctaaaa gtgttgcctg aaacgatggc
    30781 ttggcgcgtg atgttagggc tcgctgctgt ccccgcgctg attttatttt tcggtgtttt
    30841 agctctacca gagtcaccgc gttttcttat gcaatctggc cgcttggaag aggcaaaaag
    30901 agtattgaac tatattcgga caccaaaaga agctgaacag gaattcgaac aaatccagct
    30961 gaatgtcaaa caagaaaaga caaccggaac ttcttggcat actttgtttt tagaaaaata
    31021 tcgttcctta gtcattgccg gtattggtgt agcagtattc cagcaatttc aaggagctaa
    31081 tgctattttt tactatattc cgctgatcgt agaaaaagcg actgggcatg cagcgagcga
    31141 tgcattgatg tggccgatca tccaaggaat cattttagtt gctggttctt tgctcttttt
    31201 ggttattgca gacaagttca atcggcgaac tttattgaaa ataggcggta gcgtgatggg
    31261 actttcattc atcctaccag ctgttttagg aactgtactg gatgctcata caaattcgtt
    31321 gttgattctc ttgtttttat gtatttatgt cgcattttat tcttgcacat gggctccact
    31381 gacatgggta atcataggcg aaatattccc actagctgtc cgcgggcgag catctggcct
    31441 agcctcttct tttaattgga ttggttcttt cctagttggc ttgctttttc ctgtgatgac
    31501 tgcttctatg tctcaagaaa tcgttttcgg catttttggg atcatctgct ttttaggggt
    31561 tttatttatt caagagatcg taccagaaac tcgtgggaaa tctttagagg aaatcgaaca
    31621 aagtgcatct aaaaaaactt atcctaaacg tatttctatc catcattctt aaagaggttg
    31681 tgacagaagt ggtcagcttc aagaaataag aaggaaccgc ttagtttcca gtcacaagga
    31741 acaatttcaa aaaacagctt ctcatgtttg atgaatattg tgacttgtga ccagttggta
    31801 ggagaacacc gtttagtgga gaggtggtga aagaaacgtt cagccttgag cgatgacaaa
    31861 agctctcaca aacattgcga cgagtaaccg taggagcaat aagaggcggt aaaaacccgt
    31921 ctcttatttt tcgttttctt tcactgtttc ccgttcaact agtacaggtt catagagaat
    31981 atttggctgt gcctgtcctt tttcaatcgt tgtgacgatc caccgtgcgg catcttgtcc
    32041 caacttttct tttggatgcg tcaatgttgt caatttttta ttccctaaat ggcttaaaga
    32101 agaatcgtct gttcctacga tcgataggtc atctggaata tgatagccat tttctgtcaa
    32161 cttatctagc attaaactag ctatttgatc gttatagcaa atgatgccat ctaaagcttg
    32221 ttcttctttc aaccgttgta ctgttttttc agcgattgtt tctcgtgttt ccgtggtgaa
    32281 agtaatgata tcttccgtca aaaaacgcac attatactgt tcacacgcgc tgataaaacc
    32341 tttcatcctg tattttcctt gaagatcgtc gatttttgtg atgaacaaca gacgctgatg
    32401 tccttttttt atcaaatatt ctgctgccat aaaaccgact ttcacatcat ctaaacaaat
    32461 ataagaggca tttaactctt cataagcagc attgatcatc accatcggaa tgccttgttc
    32521 tctcaaagca acataaagtg ctaagtttgg gttatactga ttacttttcg taggctcaac
    32581 gatcaaacca tctaccccat aagcaatcat tttcttcaaa cactctttct ccaattcgtg
    32641 atcgttattc gtactggcta aaagcaatga atacccagat tctcgtaatt cattttcgat
    32701 tcctcggata atcgacggaa aaatataatc agagagatat gtggtgatga caccgatcgt
    32761 ttttgttctg ttcaatggtg ttttttcttt cttatagctg tcatctacat atgtgcccga
    32821 ccctttttct ttacgcagat agccctcatt taccaacaca gcgattgctt gccgaatcgt
    32881 atgtctgctg acgtcatata tttcttgcag cttgaattct gagggaatgg ttgtatttac
    32941 ttcgtattcc tcacgaagga tcttttgttt tatctcttct gcaatatctt gatattttgc
    33001 catttgcctt gccccttttt atttgtacgt acatatatca taacgagtct gcaaggttta
    33061 ttcaagcaaa aatcaaggtt tcttgtgctc ataaggcata cggaacaggt tcaccaaatt
    33121 ctggaaatcc ttgctcattc cacgtaaagc attgcgcgtt cgcgtgccga ttaggattgt
    33181 ctaacggatc ccctttttgg ttcttctctg gtcgagcatg atagatcaaa atatcaaagc
    33241 acccgtcttc tgaaaccgta aacgagttgt gaccaggacc aaattgctta tttttttcag
    33301 aagaaacgaa tactggctcc gataatttat gccatgattc ttttaataaa agattcttgt
    33361 gctcatcagc ccacagcagg cccatggcat agttttcatc tgttgcacta ccagaatacg
    33421 taatgaatat tttcccattg cgagtaagga ctgctggacc ttcatttact gaaaaacccc
    33481 gtttttccca ttcatactca ggtatagaaa ggaggatctg cttccccttt aacgtccaag
    33541 gattatccat ttcggataga taaagattag aattcccagt aattgcagga tcttgttggg
    33601 cccacacata atatagcttt ccttgagaag agaaagttgt tccgtctaaa ctgaaagttt
    33661 cccttttcgt ttttatttgt cccttttcta tccaatctgt attcattgga tctatatgct
    33721 gattttcaag tacgaacatg cggtgatgat gagattcatc acgtatagtt ttatgattgc
    33781 ttgcagcaaa gtaaatatac cattttccat tgatataatg gacttcaggt gcccagatca
    33841 attcactcat cggtccttct tcatgagcct gccatacaat caggcttgca gcatgttcta
    33901 attcattcag tgactttgcc cttcttaatt caatcacttg gtagcctgga accgatcctg
    33961 taaaataata ataaccatct gtatgtttat atacccaagg atctgcccgt tgccgaacga
    34021 taggattatt ataaagtgtc ttcatcataa caaatttcct cacattttct ttattctaat
    34081 tacattccaa gagtattttt ctatccctac atttaattcg tcatgttttt ctattgaaaa
    34141 atagtttatt tcaggtttta ttttttctgg ttcttcacgt gtatttttgt ccaacagatc
    34201 gctactggtt aatgtgtagt gctctccttg atctagcaaa ttgaaatcat gttcaatcgt
    34261 tatcttttgt tgttcatttg tgcgattgat caagaaaata gtcagctgat tgccgttatc
    34321 tacgatcaca ctgtccacat aaggaacatt ttttatcttt tcacaatcat aaacagggca
    34381 aatgatttgt tgtttcaata cttgaccttt agcatatttc gataagtgtt gcatcacata
    34441 ataagttgga ttcctccatg cttgtccttt agcatctgtc agtattaaag gtattgtgtt
    34501 tactaacaat gattgacaac taatctttac gcgatctgcg tgtcgtaaaa tagccagccc
    34561 taatgaacca aataataatg tatcttccat agtaaagtaa caccaatcga tgggactacc
    34621 gacttcaaaa tccctatgtc ttttttccgg ttgggcatgt acattccatt catcaaacgc
    34681 tagatacatt gttttctcac ttctcaaata tccttttaca taatcacaag tagcaacgat
    34741 ttcttcaatt tgacgatcga atcgataagc ttttgcaagg tatgtcgctg tatcatcacg
    34801 ctcattttta ggaggtttag tcaagtcttc ttcatcatac ttatcaatat agttatgcaa
    34861 agctaaataa tctacattct cataagcttc ttctaatgcg atacgatccc attcagggta
    34921 cgatggtaaa cgtggcgtag agcttccaac taagattgtt tcaattgaat catccactaa
    34981 cttcatgact tttgaggctt catttgctaa cctaccatat tcataagcac tcttagcccc
    35041 cacttgccat ggaccgtcta gctcattccc taaacaccat aatttaacgc caaaaggctt
    35101 tttttgccca ttttttattc ttaaatcact ccagaaggtt ccttttggaa aattacaata
    35161 ttctactaaa ttttttgctt catcaattcc ttttgttcct aaattcaccg tgaatatcgg
    35221 tttcgcttgt acatgactga tccatttaaa aaattcgtgg agtccaaaag aatttggttc
    35281 tatacctcgc catgctaaat caattttttt tggtcgttgt tctttcctac ctacagtatc
    35341 cacccaatca taaccagatg taaagtttcc tccaggatag cgaatgacac ctaattctaa
    35401 ttcctttaca taagagagga catcttgtct aaacccgtca ccatcagctg tttcatggcc
    35461 cggttcataa atccctgtat agacgacact tcccatatgc tcaacaaatg agccaaacag
    35521 ccgagaactg atctctccta ccgtaaaatc ctctctgatt tttactttca cttcttcgtc
    35581 caacagcttt ccctaccttt catttcctat agtcaagata atagatacaa gtttccatac
    35641 gccaccagca aagataaaaa taaaaggagg gaaaagaaat atgagcagta caaatcctaa
    35701 taacaatacg caacaaagaa tatttctcaa aaaatcaaaa aatactttcc atgcactttg
    35761 ttcaaaaatt tgtaaaaatg tgatatcttt gttttcagaa atcacatata tccaggataa
    35821 aagaaacatg gaataaataa gtaaaactac atatccacta attgaaagta cgattgtctg
    35881 ttcttctaat aattgattaa caagaaaata aataatgatc gttaacagac taaggaacaa
    35941 gttcttacca aaatgttgct ttattccttg aaagaaaaaa taaatgatat ttcctgatcc
    36001 ctcacctttc cattgcgcca ttacataaaa aacagcacat gtagcaggca cgatagtaat
    36061 aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag aaaccacaat
    36121 caactgtcca taaaatttag tctcaactat tttcatcttc ctcactcttt cattccagac
    36181 atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag taatggcagc
    36241 attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg ccctgtaaat
    36301 aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat caatggataa
    36361 agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag agtaggtttg
    36421 ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc aatgcgtgct
    36481 gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa gataccaaat
    36541 gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc aagcaaattg
    36601 acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt cagcaaaacg
    36661 aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata ccccgcaaga
    36721 gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt atttttgatg
    36781 taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt atagctatct
    36841 gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga agaaataatc
    36901 agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat cgttcgaatg
    36961 atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga gctttctggt
    37021 gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt acacattagt
    37081 ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg atgcatagcc
    37141 caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg tttctgtctt
    37201 aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca tcgaaccaat
    37261 cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg taatgttaaa
    37321 aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag agggagagat
    37381 tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc aaacagtcat
    37441 gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga tcggcaagcc
    37501 gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca tagaaaaaat
    37561 aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac gacaaaaacg
    37621 attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga taatttgtgt
    37681 tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg ctcgttcatc
    37741 actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa agaaaatatt
    37801 caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa ataataataa
    37861 aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat tccaatattt
    37921 ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata caatcttggt
    37981 ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat aacacctatc
    38041 tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta tcctgtataa
    38101 agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt tcatgagtgt
    38161 ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat cgttattgat
    38221 cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt gtttcgataa
    38281 ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat ccaagtaggc
    38341 aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc aatatttcaa
    38401 aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg catatcctac
    38461 gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg tagtcacatt
    38521 ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga tttcaatggc
    38581 tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt ctggaccagt
    38641 cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct caggagacgc
    38701 aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta acatccagtt
    38761 atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt tactactatc
    38821 agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt gttcagtatc
    38881 taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt gtacctgcat
    38941 ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt ctttaaaatc
    39001 agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg ctccgttttc
    39061 tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt ttttagaaga
    39121 aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca tttcaatttt
    39181 gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata tttcaccatc
    39241 agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat cagattctgc
    39301 tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca aagagccaat
    39361 taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga catctgacca
    39421 cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct tttacatttg
    39481 ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt gtatcaaatc
    39541 cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag tgaacagtta
    39601 gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg tcatttatta
    39661 cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag tttgctgaac
    39721 gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata aatggataat
    39781 agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca ataacgttta
    39841 ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa gtgattaata
    39901 gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa ggaggagcaa
    39961 tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata ttccattcat
    40021 caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta tcgcacatcg
    40081 ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa tgagaaagat
    40141 catttttttg gttgccataa tattgatgca gagacaaata atctatttga tcgtacgctt
    40201 gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc gaacttccgc
    40261 ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt tcctctgcta
    40321 accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc atttcattcc
    40381 ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc cttaaatcac
    40441 tccaataagt tcctttagga aaattacaat attcgactaa attacgagct gcatctactc
    40501 cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga caccagttat
    40561 aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa tccaaccttc
    40621 taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac acaaaatttc
    40681 caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg acgtcttggc
    40741 gaaatccact tttattagct gatggatgat ctggctgata aatcccttca tatactgctc
    40801 gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct acacgcttga
    40861 cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata catgaataat
    40921 acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt ttttatcgat
    40981 tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa cttttattaa
    41041 caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata
    41101 aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc
    41161 cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc
    41221 agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa
    41281 aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat tagttttcca
    41341 gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca
    41401 aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg
    41461 gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc
    41521 agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg
    41581 ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca
    41641 gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt
    41701 gctgatatcc gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc
    41761 ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca
    41821 aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg
    41881 aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac
    41941 gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa
    42001 acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa
    42061 atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc
    42121 gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt
    42181 atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc
    42241 agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc
    42301 ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt
    42361 gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc
    42421 tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa
    42481 atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt
    42541 gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc
    42601 ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt
    42661 tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc
    42721 ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc
    42781 tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc
    42841 taattcattg ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga
    42901 agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca
    42961 gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt
    43021 gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact
    43081 ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt
    43141 ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct
    43201 tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc
    43261 cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta
    43321 tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct
    43381 ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt
    43441 tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct
    43501 tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct
    43561 ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga
    43621 atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct
    43681 ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac
    43741 gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt
    43801 gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct
    43861 tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga
    43921 gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact
    43981 aattggcagt gcgctaaagg cccttccact tggatcaatg gttcatttgc aaaaactagt
    44041 tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa
    44101 aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc
    44161 agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta
    44221 aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc
    44281 cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct
    44341 gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta
    44401 taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt
    44461 acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc ttattggtat
    44521 ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta
    44581 gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg
    44641 ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa
    44701 aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat
    44761 ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg
    44821 ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca
    44881 gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta
    44941 ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata
    45001 gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt
    45061 attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc
    45121 atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta
    45181 ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct
    45241 aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta
    45301 ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca
    45361 ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta
    45421 gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact
    45481 taatagtata acataatttc atgagaaaaa aatacaattt tttcttgacc atctcgttac
    45541 gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa
    45601 tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc
    45661 taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc
    45721 gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg
    45781 tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc
    45841 tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt
    45901 acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt
    45961 tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg
    46021 ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa
    46081 ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa
    46141 gcttagcaga ccgtatcttc caagctcata agaagtggtt atcttacagc tggcctgaat
    46201 atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg
    46261 catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa
    46321 aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca
    46381 aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc
    46441 acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt
    46501 cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag
    46561 atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat
    46621 caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga
    46681 atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt gttttccttt
    46741 ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg
    46801 acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat
    46861 gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc
    46921 tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg
    46981 tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa
    47041 tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt ctttctgata
    47101 gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact
    47161 gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt
    47221 ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac
    47281 cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga
    47341 aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc
    47401 ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca
    47461 aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata
    47521 tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac
    47581 ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa
    47641 ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat
    47701 actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa
    47761 gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg
    47821 atactgtaaa tatatcttta ttattagtga atggatattt acttttaata gataaaatat
    47881 tataactatt aaaaataact attttcatta taattacaca caaaaaagag cgtgagacaa
    47941 aagtaaagaa ca
//
LOCUS       DACDTO010000014        44847 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDTO010000014 DACDTO010000000
VERSION     DACDTO010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.014"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            193..1518
                     /locus_tag="IWK70_000818"
     CDS             193..1518
                     /locus_tag="IWK70_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9613386.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            1511..1861
                     /locus_tag="IWK70_000819"
     CDS             1511..1861
                     /locus_tag="IWK70_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613387.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1858..2037
                     /locus_tag="IWK70_000820"
     CDS             1858..2037
                     /locus_tag="IWK70_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613388.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            2173..2463
                     /locus_tag="IWK70_000821"
     CDS             2173..2463
                     /locus_tag="IWK70_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9613389.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            2831..3619
                     /locus_tag="IWK70_000822"
     CDS             2831..3619
                     /locus_tag="IWK70_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9613390.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            3606..3932
                     /locus_tag="IWK70_000823"
     CDS             3606..3932
                     /locus_tag="IWK70_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613391.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            4281..5321
                     /locus_tag="IWK70_000824"
     CDS             4281..5321
                     /locus_tag="IWK70_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9613392.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            6157..6489
                     /locus_tag="IWK70_000825"
     CDS             6157..6489
                     /locus_tag="IWK70_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9613393.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            7080..7409
                     /locus_tag="IWK70_000826"
     CDS             7080..7409
                     /locus_tag="IWK70_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613394.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            7396..7737
                     /locus_tag="IWK70_000827"
     CDS             7396..7737
                     /locus_tag="IWK70_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9613395.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            7851..8090
                     /locus_tag="IWK70_000828"
     CDS             7851..8090
                     /locus_tag="IWK70_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613396.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            8157..8396
                     /locus_tag="IWK70_000829"
     CDS             8157..8396
                     /locus_tag="IWK70_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613397.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            8482..9153
                     /locus_tag="IWK70_000830"
     CDS             8482..9153
                     /locus_tag="IWK70_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9613398.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            9206..11182
                     /locus_tag="IWK70_000831"
     CDS             9206..11182
                     /locus_tag="IWK70_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9613399.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            11195..11851
                     /locus_tag="IWK70_000832"
     CDS             11195..11851
                     /locus_tag="IWK70_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9613400.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            11947..12774
                     /locus_tag="IWK70_000833"
     CDS             11947..12774
                     /locus_tag="IWK70_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613401.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            12784..13032
                     /locus_tag="IWK70_000834"
     CDS             12784..13032
                     /locus_tag="IWK70_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613402.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            13063..15174
                     /locus_tag="IWK70_000835"
     CDS             13063..15174
                     /locus_tag="IWK70_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9613403.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(15212..17269)
                     /locus_tag="IWK70_000836"
     CDS             complement(15212..17269)
                     /locus_tag="IWK70_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9613404.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(17383..17595)
                     /locus_tag="IWK70_000837"
     CDS             complement(17383..17595)
                     /locus_tag="IWK70_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613405.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            17782..18249
                     /locus_tag="IWK70_000838"
     CDS             17782..18249
                     /locus_tag="IWK70_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613406.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            18246..18488
                     /locus_tag="IWK70_000839"
     CDS             18246..18488
                     /locus_tag="IWK70_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613407.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18506..18808
                     /locus_tag="IWK70_000840"
     CDS             18506..18808
                     /locus_tag="IWK70_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613408.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            18879..20933
                     /locus_tag="IWK70_000841"
     CDS             18879..20933
                     /locus_tag="IWK70_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613409.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            20933..22765
                     /locus_tag="IWK70_000842"
     CDS             20933..22765
                     /locus_tag="IWK70_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9613410.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            23460..25376
                     /gene="ltrA"
                     /locus_tag="IWK70_000843"
     CDS             23460..25376
                     /gene="ltrA"
                     /locus_tag="IWK70_000843"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9613411.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            25493..26293
                     /locus_tag="IWK70_000844"
                     /pseudo
     CDS             25493..26293
                     /locus_tag="IWK70_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            26290..28821
                     /locus_tag="IWK70_000845"
     CDS             26290..28821
                     /locus_tag="IWK70_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613412.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            28871..29203
                     /locus_tag="IWK70_000846"
     CDS             28871..29203
                     /locus_tag="IWK70_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613413.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            29204..29797
                     /locus_tag="IWK70_000847"
     CDS             29204..29797
                     /locus_tag="IWK70_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613414.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            29960..31654
                     /locus_tag="IWK70_000848"
     CDS             29960..31654
                     /locus_tag="IWK70_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9613415.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            32342..34219
                     /gene="ltrA"
                     /locus_tag="IWK70_000849"
     CDS             32342..34219
                     /gene="ltrA"
                     /locus_tag="IWK70_000849"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9613416.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            34350..34727
                     /locus_tag="IWK70_000850"
                     /pseudo
     CDS             34350..34727
                     /locus_tag="IWK70_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            34727..35932
                     /locus_tag="IWK70_000851"
     CDS             34727..35932
                     /locus_tag="IWK70_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9613417.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            35948..36592
                     /locus_tag="IWK70_000852"
     CDS             35948..36592
                     /locus_tag="IWK70_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613418.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            36603..37409
                     /locus_tag="IWK70_000853"
     CDS             36603..37409
                     /locus_tag="IWK70_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613419.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            37431..37661
                     /locus_tag="IWK70_000854"
     CDS             37431..37661
                     /locus_tag="IWK70_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613420.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            38207..38482
                     /locus_tag="IWK70_000855"
     CDS             38207..38482
                     /locus_tag="IWK70_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613421.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            38524..38946
                     /locus_tag="IWK70_000856"
     CDS             38524..38946
                     /locus_tag="IWK70_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613422.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            38965..40557
                     /locus_tag="IWK70_000857"
     CDS             38965..40557
                     /locus_tag="IWK70_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613423.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            40569..40904
                     /locus_tag="IWK70_000858"
     CDS             40569..40904
                     /locus_tag="IWK70_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613424.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            41051..41245
                     /locus_tag="IWK70_000859"
     CDS             41051..41245
                     /locus_tag="IWK70_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613425.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            41277..43649
                     /locus_tag="IWK70_000860"
     CDS             41277..43649
                     /locus_tag="IWK70_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613426.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DQTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     ATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            43710..>44847
                     /locus_tag="IWK70_000861"
     CDS             43710..>44847
                     /locus_tag="IWK70_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9613427.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQL"
BASE COUNT        16589 a         6926 c         9047 g        12285 t
ORIGIN      
        1 actcttttag agtgagagtt tttattagat tttcaaatta attagtaaag tgatagaata
       61 aattgatcat acaaatcata agaagaagca gggtgatagc atacctatgc ttcttttttt
      121 atgtatttag ctatttcttc tttacaacat cgaacatatg tttgtataat gtggaagaat
      181 ggagtgttat ttatgatgga ttattcaaaa gagccagtaa atgattactt tttaatcgat
      241 atgaaaagct tttacgcaag tgtcgaatgt atagaaagaa atttagatcc attaacaaca
      301 gaacttgttg ttatgagtcg atctgataat actggttcag gattgatatt agcttcttct
      361 cctgaagcaa aaaagcggta tggtattaca aatgtgagta gaccacgtga cttaccacaa
      421 ccatttccta aaacactaca cgttgttcca ccacgtatga acctatatat caagcgaaat
      481 atgcaggtaa ataatatttt cagaagatat gtggctgatg aagatctact gatttactcg
      541 atcgatgaat cgattttaaa agtgacaaaa tcactgaatc tttttacgac tgaagaaaca
      601 cgaagtcagc gaaggaagaa gctcgctcaa atgatccaag aacgtattaa agaagagtta
      661 ggtttgattg ctacggtagg cgtcggcgat aatccccttt tggctaaatt agccttagac
      721 aatgaagcaa agcataatga aggatttatt gcggaatgga cgtacgaaaa cgttcctgag
      781 aaagtatgga atattcccga aatgacggac ttttggggaa ttggttctcg aatgaaaaaa
      841 aggctcaacc aaatgggaat tttgagcatt agagaccttg ctaactggaa tccttatacg
      901 attaagaata gattaggtgt gattggatta cagctgtact tccatgctaa cggaatcgat
      961 cgaacagata tagcaatccc gccagaaccg acaaaggaaa aatcttatgg taattcacaa
     1021 gtgttaccac gagattatac tagaagaaat gaaatcgagc tggtggtaaa agaaatggcg
     1081 gaacaagtag ccattcgtat caggcagcat aattgtaaaa caggatgtgt tcatttaaac
     1141 attggtacgt cgatcttaga aacgagacca ggattttcac accagatgaa aatacctatc
     1201 acagataata caaaggaatt gcagaattat tgtcttttct tgtttgataa gtattatgaa
     1261 ggacaagaag taaggcacgt tggaataacc tatagcaagc ttgtgtacac cgattcgctt
     1321 cagctggatt tatttagtga tccacaaaag caaattaacg aagaaaattt agataaaatt
     1381 attgacaaga ttcggcaaaa atatggtttt acgtcgattg tacatgcttc ttcaatgtta
     1441 gaaagcgcta gaagcattac tagaagcaca cttgttggtg gtcatgcggg cggaaatggg
     1501 ggaattaaaa atgattgatt acaatgatcg tggacgttta aagtgggcag ggttttattt
     1561 atctgatcat actgaagaaa ttgatgccga caatgaacaa cgaaccagtc aaaatttagc
     1621 aaaagaacaa atgacgactg aagagattgc ggaagtattg aacgctgcag tcataaaaaa
     1681 tagatcagta tctattcaaa aagaagaacg aaacgctgaa ggatactatc cagaagatat
     1741 tgttggcaag atcttaggta gtgatgagct ggggatctac gttggaaaag acaaaattca
     1801 ctacgacgaa atacgtcata tcgaatttta ccaggataat aaatggagtc gattagaatg
     1861 aacattatcc agcaatatga gctaaagtat ataacctttg atcagctatc agaagagatt
     1921 tggggttatg gccaacgttt gataaatgag gttggtgttg agcgcttctc tttctatgta
     1981 gaagctgcag caggttatca taattttagg ttttatattt tcccgttata tatataaggt
     2041 attttacgga ttctatagag ttatctctat gaatcctttt ttgtttttac aaataggagt
     2101 agatactaga ataatgcgtg tgttaaacta ttattgcaaa cgcaataata aaagaaagta
     2161 ggcttacgca ccatgtctat caatacattt gtttactcgc acccgatcaa tgtttacatt
     2221 atcaagaatt taggaatcac tgtagaacaa ttttgtgagt tatatgccta cccacaagga
     2281 acggttgcaa gctggataac ccgacaacgt agaataaaat cattacctgc ttcattcgtt
     2341 tatgatttaa gcctggcctc tagtttaaac atgtctgatg tttatgaaaa gctcctgtct
     2401 ttagaaaaag aatatgattc cttcaagatt aaacatgata aaaaaataaa aaaacatata
     2461 taatatcaat cacaattgta taatattacg atatcttaat atcatgatat cgtaatattg
     2521 tttttttcta agaaaatgca ctataagaag ggaattatta gtaacaatat agacagttta
     2581 ataatgttta aattattcta gttgctgctc gcaaaacata tttcttatga ttaataaaaa
     2641 attttaaagt aataaaaagt gattatttta ctttcgaata acatatgaat aatctttata
     2701 tccgaaccga tatatatctt taaaacaaaa taatcacaca atattacaat ataatgcata
     2761 tacgatattg caatatcaca atattatgat ataattatat tataatataa gatgaatggg
     2821 ggatatttta atggcaagaa tattgtcagt agccaatcaa aaaggtggag tttctaaaac
     2881 tacgactagc gttaatttag cagcatgttt gaaatttgtt tacggtaaaa atgtactttt
     2941 agtggacatt gatcctcagg gaaatgctac agataatgta ggtttcgata tagatggaac
     3001 aaaccaacct acaatttatg aagtattaaa aggggaggct gacattacag atgctattct
     3061 ggactataaa ggaattgatg ttttgccagc cgacatagcc ctttcttctg cagagaggga
     3121 atttactcaa gtaggaagtg aacacaggct taaaagagta cttcaaccaa tcaaggacaa
     3181 ctatgattat attataattg attgtccacc aagtttagga atgcttacag tcaacgcatt
     3241 tactgtttct gatgaaatta tcattccagt tgaagctgcg tatttctcat taaaaggact
     3301 aataaagtta agtgaaacta ttgaaacggt aaaagaatat acgaacgaca agcttgaagt
     3361 acgaggagtc ctatttacta agtataataa gcgttataac atttctaaag aaatgacgac
     3421 tgcagcaacc gaaatttcta gagtcattgg tgcaaaaatt ttcaaaactt atattcgtag
     3481 aacaataacc gttgacgaag cgcaagctgc tggatcagat ctaatcaatt ttaataaacc
     3541 ctcaacagct gaagatgact ataaagcatt tacagaagaa tatttaaaag aagttggtga
     3601 ttttgatggc agaagataat cgcagtcaag ctgcagtaag tagactacta ggaaataaaa
     3661 ataaaaagaa ctacaaacca aaagaagtag aagaaatcga attaaccaag aaaaatataa
     3721 gtaaagtcat aaaagatcaa ggaagtagtg taagaacagc agatagcatt cgtataaatc
     3781 caattctaat gtcagcactc aaatattgga cgactattgc tgaatcagac aaaagtaaac
     3841 cagacgttat agaagatgcg ctattaaaat ccatacctga agaatacttg attgaaggat
     3901 ataaactagc aaagaaacaa aataaaattt aatttacatt caatatcacg atatcataat
     3961 atcgtgatat tttttttata aaaatcagta gtatcaaagc tgataagtcg cttatcttca
     4021 tattgtaata ttacgatatc gtgatataat gttattgtaa tatcgtgata tagttaccat
     4081 gagcttaaac cctgaaatag agcggtttgc tttaatttat tttttaaggt aaactaaaaa
     4141 aagataaaaa aagactactg ctttaacaaa gtttggcgac ttatcagcag tagaaaaata
     4201 tatagtggat agatatattt gtttaattca ttgtaacata gttttgaaca aaacgctatc
     4261 ctacagaaag aggattggct atgaatgatt ttaattatta caaatcaaaa gaaatttatc
     4321 gtgaaaaata ttatcaaatg cctaaggtat tttttactaa cgaaaaatac atggatcttt
     4381 caaatgatgc aaaaattgct tatatgctac ttaaagatcg atttgattat tcagtcaaaa
     4441 ataactgggt agattctgat gataatatat tcttcatatt cacagttgaa gaattaatga
     4501 aattgttaca atgccgcgaa ggtaaagtat ctaagatcaa aaaagaatta gaaaaagcag
     4561 gtcttctgaa acagaagaga ggaagagtaa ataaacgtga tggtaagatt gaatctatgc
     4621 ctaatttatt gtacttaggc aagccagaag ttacgaatca agacgtcttt aaaatcatgg
     4681 aagaggaaga caatactgat tccaccgtaa ttgcgaaaat cgcaaatacg gcaaaaccgc
     4741 gaaaaatcaa tgattctacc gtaattgcga aaatcgcaaa tacggcaaaa tccagtcata
     4801 tcaaggactc aaccgtaatt gcgaaaatcg cagacaatct attctattct aattctatag
     4861 acactaatag acacataata gacactgaaa ctgaccagtt acaaaatcaa gttttgctag
     4921 ataacttcgt cgacatcatg caagaggaca gtataaatac atttgtacca gaaaatgtat
     4981 taaacttgat aaaaacattt tcttcaagtt acagtgaagc tcaacaaacc gtccaaacta
     5041 ttcataatgc aaagaaaaaa gctgaagaat tagagcaaac tattgtcgtt tatgaagaac
     5101 tggaatctta tggaattgat gctgacaaag gattatatat gactttactg aaagcttatc
     5161 aaaaacaaaa aactgaaaaa gtaaacaatt tgcagaatct aattttcgtt tatgtaaaaa
     5221 attggtttgt cgaaaaagct attgcggcaa agcttgcaaa cgaacaaaga gaaacattta
     5281 acgaattacc aacagttagt acggaaaatt ggttagagta gttgataaaa agcaattact
     5341 ataaaaatga tcaattttaa attagaactt ttttatgatt ttaattttat tacaaatatt
     5401 acaaataaat agttagtatt ccggagtttt tagattaaaa gtttatgaaa acaatcacaa
     5461 tcgtaggttt taaattcata atcgttagat attttagtta tttttcactc aaaaaaatag
     5521 ggaactagtg tttgcatatg tgcttttttg agttataata attcatgaag agaggtaatt
     5581 cacttatttc cccaagctga aaagcctgat attacttctt ttcaggcagt gctgctaata
     5641 ttcaattaca cttttttgta tatttttagt tgttttaaat ttaattgtct gctatgcttg
     5701 tgatgaattg attttcgttc tgcacttttg gacgataaat ttggacaatt gaaaatacaa
     5761 aaaaaagaaa ggccctgtga gactcggaag gctggtaacc ttcgtcgacg tccacaggtg
     5821 gcattaagat cactccctta atgcgtttac ctttcagtgt tacggtatta cccgtaacgg
     5881 atttagtata acagatattc aaaagtgata ccactttttt tggctgtgtt aaggttaaaa
     5941 atacgtgata ttacgatgaa tctatgccta agcataatgt tataaaaatg tccgtgtggc
     6001 tctttgccgt atgacgaacg ctgaaattag agggaacgca tgtttggatg cgttccctct
     6061 tttttgtttg tcatgaacga aaatcaattc tctatttttg aaaaataaca agaaaaactt
     6121 gttaaaaata tttaaagtaa gtgggtggat tattttatgt catatacagg aattttgagt
     6181 ctcgaagata tttgtcacta cggtaaacgg tgtaccgcta cagaaaagat cacgaagaaa
     6241 ctatcaactg gtcaaagtaa aaccgttgtg cagtgtaaga aatatataat ccagaaagac
     6301 aaagtctcag aagaaatgat ttattacatt gggaaacaaa aacaaataat tttaaaagat
     6361 cctatccctt taaaagagct atatccaaca atcaaacatg tttatgacca aaacggtgta
     6421 ctgattggtc gaagaaaaaa tggagtcttg cgttgtaccg caaaagggat ggggcgttta
     6481 atcagctgaa ttgatcattc tcataacttg tattttatcg tttttcgctt gcttgaaaat
     6541 attgtcgaga cttctaaagg aacttagaat cactagaaaa ataaggagta gacttgatct
     6601 atcaacaatt cctttcgggg ggtgacgggg taggttttta cgattggtaa cgtaaaaaat
     6661 tggtcaatcg agtaaatgcc cttgtttgga tagtggtgaa atgaatttgt gtaaataata
     6721 tgcacagagt aaacaagagg gaatttagaa gttagacaag ttagaatagc gcctagatta
     6781 atcatgagca gctactaagg gatctaggct ttgacgcaaa ctcatggaat aaggagtagg
     6841 tcttctcgta atggggagga cgcacaagaa attgtgtcgt gtgctgcagt acatgtagat
     6901 acttgcgaag acgttgatct ttggatcaat cgagcattta ccgttttcta ctagggtttc
     6961 tcgtttcgga cgccataaac gagtcgattt attttatata tttttgagtg aacgaaaaac
     7021 gataaaaata ttcgttatga agtaaaacta atttagtaga tagcaaaggt ggtagaggaa
     7081 tgattcaaaa atttgcgaaa atgagtaaaa ctgcatggat tgaaaagctt gaaaatgact
     7141 tgagtgtcat gcgattagct ccaaagaaat tcaaacgaga gattgcagaa aaagaagcaa
     7201 ttttgtcggt attgaatgcg gtagacgata aatgtccagt aaaaattagt ttttcagata
     7261 tagcaacaat tatcgaaggt atttattatg gtgaagttga tgaagagata tcttctacaa
     7321 aagatttatt agattttttg gataaaaaca aagatacctt tatagtacta actgattatg
     7381 aggaggttac aacaaatgaa aaattatgat ccgaatagcc gatggggaac acatacgatt
     7441 aaggtttctt tccaacgttg ggattacaaa ggatttgtga cgtttagaag aggcggaaat
     7501 tgtaaaggat tagatgtttt agcattagac gaggatgatt tatacgatca aaaattgact
     7561 gataatccaa ttggattcgg cttgctacct gaagatgatc aaggtaatga atggtttaaa
     7621 atgacgctta tgaatgataa tggagacgaa ttatctgttg aagatacttg gtcatatttg
     7681 agtgattata ttgtcagcgt tgaaatcatt gaatttgtag cggataaaga agaataaaaa
     7741 ctggaggaat aaattttaca gataaaggct tagaaggagt cacgacgatt aataaaaagg
     7801 tagtattacc ttaggcattg agtaaggaat ttatttagga ggaaaattag atggcgcgac
     7861 cagttttaga ggcgatggat atggatactt tgacaaaagg aaggtatgta cagctgttag
     7921 ctcgtggtta ttcacctaaa gaagtattca aatccctgtc taaaggaaca gatatggaaa
     7981 aagaaagatt acgaaaagaa ttcgattatt ggagaaccca taatggaatc aaggatttga
     8041 aaccagcacg gccagtaatt aaacgaaaga aaaagataac ccaagactga agtgtagagc
     8101 gtatgtgatc ataggctttt ttatttttgc aaaaaacaag gaaggtagga aaactcatga
     8161 attatgaaga aatcgaaaac agaaaaaaag tgtcgaaaga aatggaagaa aagttgctaa
     8221 aaacgatgaa acaaaaacac cttaaaagat tatctgtcat gcaatatatc aatgatatgc
     8281 agataacagg aaaagagaaa gcttgtttgt taggttcaat gaaaaacttt gaacagttga
     8341 gacgaactta tgtgaaaaaa agttctaatt gtcaactatt gttagaagtc tcatgacatt
     8401 cattaataga attcattttt aaaacttttt ctatggaaat gagaaaaaga ttttttgttt
     8461 tgtctgaaag ggggtagctg gatgagaaaa tggttatttg gttttttagg agtcgcttta
     8521 atcgtctttt gcagtgtgtt tggctatgtt agctaccaaa aacatgaagg aaaggtgttt
     8581 aagcaaaata tagaaaaaaa gatgcccgta gatcaaatca atgaacatgc aaagagttat
     8641 aaggaggatg caacgaacgt caataatgat atgtctttgg ggcaaatgtt gtccatacaa
     8701 aaagaagcaa tcgaaatggg tgtcaataaa caagcctttg cacaaattca gatacctgca
     8761 cttggtttag cattgccaat ttttaaaggg gcaaatcaat acactttgag tcttggagcg
     8821 gctacttatt tttatgaaga tgccgaaatg ggcaaaggaa actatgtcct agcaggtcat
     8881 aacatggaaa tgccaggtgt tttattttct gatattcaaa agttgtcatt aggggaggtg
     8941 atggacttag ttagtaacga tggcgtctat cgttacaagg taacgcgtaa atttatcgta
     9001 cctgagtact ttaaattaat cgatggcgtt cctgaagaga actctttctt gtctttacca
     9061 aagaaaggag agaaaccctt attaacgttg tttacttgtg tttatacgtc acaaggaaaa
     9121 gaaagatacg tggtacaggg agagctacaa taacaaggtt ttaatcaacg cgagtcgttg
     9181 aaatatgaaa tgaggagagt aaaaaatggc aataaatcgg tatgtaaaaa aagctggaat
     9241 gcttgttgga atgttaggaa tcattgcaag ctgctttcag gggtatcaag tacacgcaga
     9301 agatgtcacg caaaaaacac caccagagaa ggtcaatatc acggttcata aattaatgta
     9361 cgatcaagga acgcaattga atgttgatat tgacgggatc aaaaacgatg gttatacgca
     9421 cgatcaatat cctacaggtg taacgaaata caacaaggca gattatggtg atgttgagtt
     9481 tacgttagga aatattacgg atcaagtatt accgagagaa gattctgatc taaccaacgc
     9541 gaaagtggac gagattgtac aagatgtaga agacaaggga tctaacagtg agtacgtcaa
     9601 gaatgcaacc aacaaaatta cttcagctgt agatgaaaat ggtgagatca cttttactga
     9661 tcaaccagcc tatgtcaatt ccaaagggaa tgtatatgtg gtttatgaaa gtaaatccgc
     9721 tgctggatta gtcacacaaa aagcaaaacc aatggttgtg attgcgccaa tgacagataa
     9781 aacaggatca ggatttttaa aaaatattca tttgtatcct aagaacattg tttctaagtt
     9841 gagttttgaa ttaacgaaat ttggtgacga tggcgcagcc caaagcaaac aaaccctttt
     9901 aaaaggggca aaatttgagt tatacaaggg cgaacctgga aaaggaacaa aattgggtga
     9961 tcttgtatcg gatgatcaag gaaagctgac tgcgactgat ttgactttag gaaaatacta
    10021 ttttgtggaa gttccttcgg aagttgtagt gggatcagat caagaaccaa cagcagatca
    10081 atatttatta ggcgcagacg ctcgcaacga tgcgcataat aaattaacct ttgaaattac
    10141 caatgacggc gtgacaagtg acttaaaagc ttcctacgtg aactataaag caccagtgat
    10201 cgataaaaca gtgacaaatg gaacaggaca agaacattct ttccaaattg gtgacgcagt
    10261 gaattatcaa ggaacgattc atatccctac agatattgca ggtggaacag atgggattac
    10321 agtaaatggt gtgaagagtg aaacttctcc ttacagcgta tttaagtggg gagatacagc
    10381 aggacaagga ctttcttatg tcgctgctaa agcaaatatc aaagtaacaa ataaagatgg
    10441 atctgtggta ttaaaagaaa atacagatta taaaatccaa aattcagaaa atggctttgt
    10501 gatcgatttt atcgtgaata atggccaagt aagtgatact gtagcaagtt tacacggaca
    10561 agatcttcaa atgacttata acatgtatgt aaatgacagc gcagctgttg ccaatccatt
    10621 aacaaatagc gtggatttcg tttataacaa caatccattt aatcaggaag aacaccacga
    10681 aaaaacgaaa gctgacgttg tgacttatgg tgcaaaattc ttaaaagtcg atagcggact
    10741 atttggaaca ggaatcaaag caacaccgct tgaaggcgca gaatttgctg ctaaaaacgc
    10801 agaaggaaaa tattatggtg gattagttga tacggataaa gacggtgtca aagaagcagt
    10861 atgggtagac gatgtagcaa atgcagcgat cctaaaatca gataaagaag gtcactttga
    10921 aatcactggt ttaactgaag gtgaatattc actggaagaa acaaaagcac caactggtta
    10981 ccaaaaatta acagaagata ttgcatttaa agtaaacaaa aactcattta aagaggaaaa
    11041 tcgaatcact gttaaaaata atcaaaaagc agctattcca ttaacaggta gtaatggttt
    11101 ccaaacgtat gtattgatct cttgcttgtt gctaggagca ggggcacttt ctgcagttgt
    11161 ttactttaag aagaaagcct agaaggatca gctaatgatt attcgtaagc ccccaaaaag
    11221 aagagtcatg cacaaactga tgatattttt ttggttgctg tcggcagtag cagtctattt
    11281 aattccagct gtggaaacaa atgtagcgaa atttcaacaa cagcaaagaa ttttagccat
    11341 tgaaaatctt gaaaaaacag attcttcttt ggatgttgcg aaaatagagt taggcgatcc
    11401 tgttggtgtt cttacaattc caagtattcc tttaaaacta ccaatttatg acggaacttc
    11461 cgacaaaatt ttagagaatg gtgtagggat tacggaagga actggcgata ttacaggagg
    11521 aaacggtaaa aatcctttaa tcgcggggca cagtggactt tataaagata atcttttcga
    11581 tgatttgcct tcagtaaaaa aagggcaaaa attctttatt aaggtaaatg aagaacaaca
    11641 tgcctatcaa atttatcgaa ttgaagaagt taaagcagaa gaattacaaa aaaattattt
    11701 agattatctc atgcctgaaa aagaccaaga taggatcacg ttaatgacat gcacgccaaa
    11761 aggtgttaac tcacaccgct ttttagtgta tggtgaacga gtgtcattca gtaaagaaga
    11821 tttaaaacta cagaaaaaag caaaaaaata ggaaatcaat ttcattggat tggcgattag
    11881 cactgctatt cgcttttttt gcgttcctat tatttttatt tataaaaaaa taaggaggag
    11941 atcggaatga ataagagaaa atggctgttt gtttgtgtag cagctctctt tgcaactgca
    12001 gcttactcac agatatcttt tgctgatagc agtactactc caactaccag tcagacaaca
    12061 gatagttcaa caactgcaac aagtgacacg gacagttcga agaacactgc agaagaaacg
    12121 gcagattcaa gtacgagttc tactgaatca gcggaatcaa cgtcagaaag ttcttcagag
    12181 acttcagaaa aactgcaaga agtagaagat acgcaagtaa tcaagcaaaa cattcatatt
    12241 caaaaaatta tttcttcgaa tcgaatcaac aatcaaggaa ggcaagtgac tcagcaagaa
    12301 ggtgtcaatg gtgcaaagtt tattgtctac gatatcacga atatcttaga agaaatgaca
    12361 caagatgata atgagaaaaa tcaatcggta gaaataattg agtcaaaatt gaaagatcga
    12421 gcaaaaaaac tttcatacga tcagttgaaa aaagtgactg aaggagaaac ccaaacaatt
    12481 gatgatcagg aaggcgtgat tgatttttca attgaagtac ctgcaaacaa aaaacaagca
    12541 tactatatcg tcaatgaatc aagccctgaa aacatttcaa atagcgaaga tattgtttta
    12601 ttgacacctg tttcagatga aaagggtgaa tttttgacgg atgtttggtt atatcctaaa
    12661 tcaaagaaat cagagccgaa agaagaaatt aaaaaagttg tatcaactgg tgtaaagaaa
    12721 aacttttttg ataattgttg ggattttttc actcatttat tttttaacga ttaggatgaa
    12781 atgatgaaaa taataaaaat gttagttttt aatctatctt ttttagccgc aacaatattg
    12841 tgttgcggtt tagttttttc tcaattatca atttacccaa gatatcaacg attgttcgtt
    12901 cattttctaa atatccagca tgcttccatg ccactagaag tgattaacaa gctcttaatc
    12961 aatccaattt ttatggctag tttgtttctt cttgcaggat tggtaacgtg ggtattagga
    13021 aaaaaagaat gagaagctta caatcgttta acaaaataat agatggaggg cattatggaa
    13081 aataaaattc attgcactac gggaagagta tttactttag tgttgttttt gttccttctt
    13141 ctttttgcaa ggggaactgc agtccaggcc ttagaaaaag ttgatccgag tcaaatcaaa
    13201 gaaaataata atctggtact ttatcatgat agtgattcat tgagaaatat cgaagtaact
    13261 gggatcaatg gatttaagat actagatgag tattcaacta aaaaacaaaa agtacttgct
    13321 tggccaaaag tcaaagattt gaaaagcacg tcaaaaattg ttgcccatta tcaaaaggtg
    13381 ggcacttatc taggaaaaga aattgattgc gacgtaactt tttctaattt tgtagcgcac
    13441 gctggggtta accaagacgg attagctaaa acaattaatg ggaaaaagta ttataattat
    13501 ttgctctttg ataaaaacat ttatcgaggc tttttcatgc agtcaatcaa cgttatgaat
    13561 gttaaactag attttaagta tgcaggatca aaaacaaaag tagctttgag tgaaccacag
    13621 ccagaaatta attcaaaaat gagatcgatg agagcagctg gtattgagtt gaatgataca
    13681 tttctatcta tttacagttt aaatggacag tatcagcaac gtgtgtcttg gtataatgac
    13741 aataatgaat ctgtcggtga acgtaatatt gatatgagag aatttgttgg gtacgaaaaa
    13801 atggatcgct taccttattt tgtcacaaca gatacagcat gtgcagaata caaagctcct
    13861 gcgttatcaa caaacaattt aacttcaaaa gtagtgggag gacgtgcaga aaaggcttat
    13921 agctcgaatg atcatttcac cgatgttgta ggagctgata cttatcacag aagtggtgta
    13981 acgtatacgc ttcaaggcgc ttccccaaca ttcatgattg gcgcaaatac gaatagtatg
    14041 atgtttagct ttgatactgc attgctttgg acaccacaac catcgaagcc tacaaaagaa
    14101 gtgtttaaca aagctaatac tgaagaggca gcacacaata ttgacaaaaa agtgattcca
    14161 caaggatcag atgtttacta tcatattcac caaaagtttg atgcattaac agtcaacaca
    14221 atgaacaaat acaaatcatt taaaatcact gatacctttg acagcaaaaa ttttgatatg
    14281 gtatcggatg ggaaaaacta tgatggcgca ttgtggtatg ttgaagcgaa tgggacttgg
    14341 aaaaaagctg atacaggagg aaaattaact gtatcaggta atacagtcac ttatactgct
    14401 tctgacagct ttttagctaa tgctgcaaat tacggtaggg actacatttt gcagctccat
    14461 atgagagcaa aaaatgatgc aaaaggaatt gcaccaaatg tggctactgt aacgtttaac
    14521 aatgatccga acaaaggaac acaaaaaaca aataaagtag aaaactattt ctctcttaaa
    14581 ccaaataaaa aagtggaaca agatgggaaa gatgttgatg gtcgaaatga tggtaaaaaa
    14641 ggaacgccta cagctccttt aaatgcagga tctgaagtac aatatctagt cacacaaaaa
    14701 tggcatacaa aaggtgtaga cattgcgctc gatcattaca agcaatttag tattcaagat
    14761 ccagtggaag ctcgtttgac gtataaagaa ggttctgcgc gagtgataga tatgagtaca
    14821 ggaaaagata ttacttcaga aggtatacta acctatgata gccattcaag aacgctgaaa
    14881 tgggaagctt ccgctgattt cttaaataac aatcttttag atggacgaga aattcaactg
    14941 atatttacag ctaaaactcc tttgaatgaa gaattggata tcgacaacca agcaaaagtt
    15001 tcagtagaaa gtattgagaa agcaactaat gtagtaacga ttggggtaaa acctaactta
    15061 cctcaggtca ttgtgccaaa aactggatcg aatcatttag tgctaactgt tattggaagt
    15121 atattatttc ttactttagc atcattgggc tatgtagttt taaaactcaa ataaatataa
    15181 aaacaaggct gattttcagc cttgttttct tttatttgaa gattggattc gcactaataa
    15241 atacattatc tacgccaatt ctaccagcag agtcaactac aaaattatga cctaatccga
    15301 gaatactttg gttattataa agtaaatttg cattatgggc agggctattt ttccatccta
    15361 ccatggcatc gatagcacca tcgtctgata aaaggtcatc aatatacagc ccagtttgac
    15421 ttatgttttc tcccttagca ccatagcagc ctaagctttc caataaatct tggaaagtac
    15481 caccaccagg gcgatagtga ctgaagctga cttgtaattc tttagctcga atatctgcag
    15541 cctgttgaac aatatcattg aattgcattg gggcaacacc attttgttgg cgataatcat
    15601 ttaaccaagc aagttctttt tgacgcataa agttacgtaa ttcgtctaat gaaatctttt
    15661 tatatttgaa tgtgattgtt ttattttgat cgacgttatt aatggatcgt tggccgtttt
    15721 tatccacggt aacggtagga tcgtcatttc tgcacaagaa ataattttga tcagtcatat
    15781 ttttccaagg agtagagaag ctatcccctt cgtatactgt ttttgattca ctagagagaa
    15841 cagcgttggt atcagcattt acatgattaa cagtcacatt aaatttacga gtgtagttga
    15901 aaatataatt ttgactttca gtcacattgt ttaaggttac tgaatttgtt ggttctaccc
    15961 gatcgtctaa aacatatcct tgaatagaag gagcagttaa agtgacactt tcaccgtgag
    16021 tgacgttgat tatctgttct ttcaaaatat caactcgccc ctcatttttg tagttgttat
    16081 tgcacaacaa attgatttga tgcacttgat tataattgaa tgtgattgtt gtattacctg
    16141 tgattgtttt gctttgagta tcattaccaa tcactttgta gctaaaatta tctttagcac
    16201 cttcgctata tttaaatgct tttgccttag cagtgtaagt agaaccagaa gtcacttttg
    16261 cttgatctgt agcaatcact tgacctgttt gattgtcaat atggttaatg gtaaccatat
    16321 attcttttgg cgtttcaggt ttgtgttgca accctttaaa tgcagcatca agactatttt
    16381 tagcattgtt tacttgtgtt tgagaaacct tagaatcgtc tagtactttt ttagcattgg
    16441 ataaagcagt ctgaaaatta ttccaagatt cttctgtaaa atctccttta gcagttgatt
    16501 ttactcggtt gtataaagct tgtaattcag ctttattcac agtaggaact ggttttttct
    16561 ttaatccttt gtaagcagaa tctaatttat ttttggcact atccacttgc ttttgagtcg
    16621 cttttgagtc atttaacact ttcttagcat tgtttaaagc ggtttggaat gcgttccagg
    16681 tattgtcggt atagtcacct tttttcgttc ccttaacgct cgtataaaga gcttctagag
    16741 ccgatttatt cactttgggt ttagaggtgt cagcgccata ccaaccgata ccttcgtatt
    16801 tccagccata gtctttggtt aaccattgta cttctttcca acttgcagtg tagtgatgtg
    16861 agccagattt tacaccagga aggaaaaggc ggtagatcgg tttattattt ttttcggcag
    16921 aacgccaggc aggcccttca taagtccagc cgtaattttt agtcaaccat ttcacttcat
    16981 cccaactagc tgtgtaatgg tgatcacgaa gacctttatt gtacaaacga tagacaagat
    17041 ggccaccgct agtcggtgct tcccaggcgg ggccttcata atttccccat tttatcttta
    17101 ctagatggtc acgttctttt ttgctagagg tgtaaaaatg ttcattatta tttggattgt
    17161 aaaggcgata catgacgtta gttgaagcag cgtcaacaga tccagcaaga ctaagtgagg
    17221 cagcaaatgt tccaatagct aaaacagttt ttggattaaa taatttcatt ttttcaactc
    17281 ctaaatttaa ttaaaaatgt ttacatgatt attatatatc ggttaaccga tatagtcaat
    17341 aaaaaataag aaaagcctta tataattttg gcttttctta ttttatttgt tagttgaagt
    17401 attaatatca acgatttctt cagccaattt atttaatttc atagcattta tgaatctcat
    17461 gttgtctata ctagagcgtt tgttggcaag gtcacttact gtagttaagg gtacgccaga
    17521 ttctatggat atagaacgaa ctgatttacc actatcaaac agcaaccatt cgattttctt
    17581 tttatcagcg tacatttcaa tgctccttct tgttttttct ttattatagc tcttttttgt
    17641 aatgaatcac aatatcaaga gaaaaatttt ctatagaaag gtggattttt atgagtattc
    17701 gttctccgtg tatccaaatg aggtttgttg ctactttcgt tatttgatat actaaaaata
    17761 aacaacgaaa gaaggaataa aatgaagaaa aagcaactat ggtttgtctc tatgtttgca
    17821 atatttttta ttattttaag tggatgttca agtaataaga aatcggaagc taaagaagat
    17881 aatagtttat tgcaaccagg aactgtttgg aaagaagaag taggtggtct tattcataat
    17941 ttaaaaatta tagatgatac tacctgggaa tattctgaaa gtgtatggca tccagatcct
    18001 gtacaaataa cagttaagag gcaaaaagac tatgaaggtt tagaaagata caaaatagta
    18061 gattctgcag gcattaggga atttataaac aaacctgatt cattatttat tgttgttccc
    18121 tatgagaagg atggcgtaaa aaaaataatt tttcttggat ctagcaagga tgagaagcga
    18181 acgaaagaga aattaattca tgatggatca caatcaaata aatacaaatt acaaaagact
    18241 agcgagtgat tgctagtctt tttatttgtc ctgaaggagg aatcacagtg gcaaaagcta
    18301 aaacgccatt tgaaaaagcc gcgtctaaaa tcttagaacg ccgcggtgta tctgaaacac
    18361 agtggaaaca agaagtcctt gaaaaagcac aatttgacct attacgggga aaagacaagg
    18421 acttagaagg atatatctat caacgtgaac gtgaaaagct tgttttcgat gaaattatta
    18481 aacactaaaa ggagcgaaca attttatgtt tgaatcaatt acaatgaatt ttttaacaca
    18541 accgaaagta gtagctgcgg ttgatatttt tagagcatta caaactgccg caactagttt
    18601 gaaaaataat ctgatgattt ttgctgctgt tgcggcagtg gcttccattg ctcttggagg
    18661 tattttcttc ttctttgggc gaagaggagc tgaaaacggg aagacacacg taggttatgt
    18721 attgttgggg gtaggagtta ttttagctgc cgcttctatt gtagtttcaa tgttctcaat
    18781 gtttggacaa accccacaag gactataaaa aaacaagtag aaggagtaaa aagtacgtgg
    18841 ctacttttta ctcctttttt tatggaagga gaatattcat gttttttacg atttcaggca
    18901 atgcaaaatt ggataaagag ttaataagaa aagcaacgaa gaaagtggga aagcgtactt
    18961 cctttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag ctacatgtgt
    19021 taggacaagt gttagaacaa gagaatcaat cttctgttaa attgactctc acgttacatg
    19081 ataaagaagg aaaaatagaa aaggctggca cagaatacgt ttttggctca ctggattatc
    19141 aaatttcaga aggtttttat cctacattga ttgatttaat ccgcctagat atatttaaag
    19201 atacggatga aaccatgagt gtggaagagt cttatcgaaa cgaaaaaatt ttacaagttt
    19261 tagaagaaga gttagaaagc gaccaaagaa aagtgaatca agtgagtgaa gtcgaagcaa
    19321 acgaagaagt accatgggaa cgcccaatct ttccaaagga tgaatcaaaa gaaaaagaga
    19381 aaggaattat tgacgaacaa gctgacattt tgactcataa atcaatattt gaggaagaga
    19441 acgaggtaat gggtcaattg gaggtagatc cctttgaaga aacaatggag gatgaagaga
    19501 cggccgaagt agaagttgat ccagttgaag ctaatgaaga cccaacatgg actgaagggg
    19561 attctcagga atatattgaa ccacttatca gtgaagaagt agagaaaaat gaaacagaga
    19621 tacaaaaggc aacggtattt gattatattt tggatcaata tccgccagag catgaatgga
    19681 tgaaagaaaa aaataaatta tttttaaatg aactttattc gaaatatcaa ttacctcaaa
    19741 cctatcaagc atttttagag agtaaagaag aattaatctc acagggtaaa ggcacacttt
    19801 tagaacagtt agagtctgtc caatcaacaa attggcatga gcttgcacaa gaagatttat
    19861 tagctacttt tgaagaacgt caacaagaaa cagatcaaga aattaatgag tatgcgacgg
    19921 aacagtcgtc gaattgggag aaagcaaaag aggaactaaa taaagaagaa cagcgagtga
    19981 ttgaagaaga agtacaaaaa attaaagagc ggtacgaaaa gaaacgtgac aatgcgtatt
    20041 ccttgtgtgt agaaaagatc aacaatttta ctcagacgaa tcaagaagca ttatcgaaag
    20101 aaaaagaaaa cttattaaaa gaacgtataa aagaacgaaa agaagaattt taccgagggt
    20161 tgcgtactga taagctaagg attagcaaag aattaaatga acaactgaat aaactctatc
    20221 aaactacgac tgaagttctg atggaaaaac acgagcagat ccaacaagaa ttacaagcac
    20281 aaattccaaa atggaaaaaa gagtatgaaa ttcaccaaca agaattgaaa gaacaagaag
    20341 aaaaacaacg gaaaaaagaa aaagagcgcg aacaattaga actaaaaaaa cgtgaacagg
    20401 atcttgcgga acaaaaaatg gcgatagaaa aacaacggct agaacaagaa aaggaaaaag
    20461 aagcagctgc tcgtaaggaa cgtcaagaac aaatggatac attgcgacaa gcacagttaa
    20521 attatctgac acatcctcaa ccagtagctg ctgttcaaac gccagtgaac aaccaagcaa
    20581 atttaactcc ttcaccccag cgttccttta acggatggat ggtgggatgt attgcaagtt
    20641 taagcttgct tctaggtgga ggaacagtat ttgcttttaa tcatatcaat caatcttcac
    20701 aggctgattc tgtagccaca acggcgtcaa tcaaggaaga agcccaggaa caagcaagaa
    20761 aagagtatga acaacaatta gaagaattat tgaatcgtac caatcagtca acaacagagt
    20821 caagcatgga aaatagcaaa gagagtgatt ctagtaaggc taaagaagga tcaagcgatt
    20881 caactgtaca atcaaacact tccgaaagtt cagaatcaaa tgaggtgaaa taatgcgtgt
    20941 tagagaaaca acgaagcgaa aagacaaacg attcaattta gaaaagaata ggtatctttc
    21001 tcatttctat cgctatactc cctcaggaga agagctacca gaaaaacatg acaaacgcat
    21061 ttcgaaaaag attttttata gtggacttgt atacctccct ttagagtatt tagtaacaag
    21121 agtaccagaa gttttcaacc gattggaaat tgttaaggga agcttacaag gaataaatct
    21181 gtttcaatta caggaaatgc cacaggtctt tcattttgat tcagctggtt tttggaatcc
    21241 atttactcct acaactagtt tgggtacacg tttagcaatt tcgacagttt gtgtggttcc
    21301 agctgtactt atgggattga aaatggacat gtggtttcgt cctctagcag atggacaaaa
    21361 aggggataat cgattaatga ccttaaaaga aatccaacag atttatccgc aaatacctga
    21421 aacaaaaact acttttccag gttatggagg tattccaatc acacattaca aaaaatattg
    21481 gtacattcaa acagatactg ttaatacgtg tattgttggt acctctcgat cagggaaagg
    21541 tcagattgaa gtgttagcca ccattgacaa tttatctcgt gcagaaaaac aaagttcaat
    21601 ggtcgttaat gatccaaaaa cagaattata tgttggtagt aaagatgaac tagaagctag
    21661 agggtatgat gtatatgcct ttaatatcac cgatccactg caatcaatga gtgataatcc
    21721 attggcactc atcgtgaaat actggaaacg aggagacata gatactgcaa cacagttaac
    21781 caatactttc acaaatacga tttattatga tgccaacgct tcagataaca aatactttaa
    21841 cgaaaacgcg cagaaagctg tcaatgcgct tatttttgcg ttgttagaac aagctgatca
    21901 aacaggagat tactccaaat tgacaccgaa taatctcgtt gagcttcttt ctgagattgg
    21961 cggttttaat tattatccag atccagacaa tgaattcaaa cagttgaatg ccttagatga
    22021 atttatgaat cagttgccac ctggaaacat tgcaaaaaaa caatatgggg caacgaaaat
    22081 tggatctgat aaagcgaagg gaaatatttt atcaacggca atcacgggat tgaatccatt
    22141 tactctaacg aaaattgcaa agatgacttc tcaatcaacg attgattata aatcggttgg
    22201 gtttccaaaa tacttagatt tgaaattaca tgaaagtctt ttaaatcaac gagtcgtagt
    22261 agaattttat cataagggaa agaaatttca tgaagaacgt gtcaaagtag gctataaagg
    22321 atttagtgaa atcaattttg attgtcattt agttgcaggt gatcaagttt gtttaaaata
    22381 cgatcatcaa ggaaaagaat atgtggcttg gtatgaattt tctcaaagag tattgaaaga
    22441 cgaagagggt aatatcgttt atcggaaaaa acgaggagaa acccatttgc cagaattaga
    22501 aaaagaagcc gttctaacat tagatgagga aaaaagtaac ttattagtag agtctattcg
    22561 gatgcactat accgataaac caatggctat ttttatgctt acacctgatt atgatccatc
    22621 taaccatatc attgttagta tttttcttag tcagttatat aaagaattat ctacgcaatg
    22681 tgttaagact aaaggagata aatgtcaccg caggattcat tttattttag atgaatttgg
    22741 gaatagtgtg acctgtttag cctaaggata aaagaggcta gttccaacga acattggaat
    22801 aactttacta tcgagaaatc aaatgacggg gtaacgccct gaagggtttc cactgatact
    22861 gcgacatgcg ttagtaggat gtgcatccaa acgcgtatga agctcgctga agtcggcaga
    22921 agggtaccgt aaggaaagac acgatacgta tctaccacgc aagtgctcta gaggtagggt
    22981 atgtacctga tatgtcgggt gtcctaaaaa gttcaataag gtgagaatga tttgattatc
    23041 tgacaaactt gcgaatggaa tgtctgaaac gaataactca tagaaatgtg agggggaagg
    23101 gggatacgtc ctctttccat tgactcgagg ttgagtaaaa atgtgtttct atgaaaaacc
    23161 ttgcatggtt aaaggctatg gagtagtcaa caggcaaaag caagcaccta agttgaaatc
    23221 tatggataga tagtaaggaa caggggaagc atctagcacg gagggagcat accgaagatg
    23281 tgtttctaag gaaaaaccga aatcggttat aacttaggtg aatagatgtg aaagtagagc
    23341 cacgaccagt gaaaatttcg taatgaaatt ggaggaacag gctcaagtcg ttatttataa
    23401 tataagaaac ctatttaaaa tcatggattc tcgtaagact aaaaaggtca cgaaccagaa
    23461 tggagcgtga taaaaccatg actaacacaa aaggaattag cttaaagaac aaacaactca
    23521 gacacgccga atactacgac atgactgaga ctttcgatga attgcacgca atgagtgttg
    23581 caaaatcaaa ttttaaaaat ctcatgaaat tcattattga tgacaataat atctttctag
    23641 cgtatagaaa tatcaaaaag aataaaggaa gtgcaacggc cggatacgat ggtgtcacta
    23701 taaaagacat tgaaaaattg gcatgtggtg actttttaac tattattaaa aggcgattta
    23761 aggattacag accaagaaaa gttaaaagag tagagatccc gaaaccaaac ggaaaacttc
    23821 gacctttagg aattccttca atatgggaca ggatagtcca gcaatgtatt ctacaaattt
    23881 tagaaccaat atgcgaagca aaatttaata agcatagcta tggattcaga ccaaattgtt
    23941 ctacagaaaa cgccatagca gattgtcttg ctagaatcaa tcaaagtaag atgaattatg
    24001 ttgtagatat tgatataaaa ggattctttg acgaagtaaa ccataccaaa cttatgcgtc
    24061 aaatatggac aatggggatt cgagataaac aactccttgt aattattcgt aagatgttaa
    24121 aggctccgat tgttatgcga aatggcaatg ttgtttttcc aaccaagggt actccacaag
    24181 gagggatttt aagtccactg cttgcgaata tatgcctgaa tgaatttgat tggtggatag
    24241 ctaatcaatg ggaggaacga actgtcaaag agctgaaaca ccactatcga aaaaacggag
    24301 catgggataa aggaaatatc tattcgaaat taagaagcca cacaagttta aaagagatgt
    24361 atctggtacg ctatgcagat gattttaaaa ttttctgccg aacaggagaa gaagcccaaa
    24421 gggcttttta tgccacacaa aaatggctca gagaaagatt aaagctccca atttcaatgg
    24481 agaaatctaa agttacgaac ctaaataaag ctgaaagtga attcttgggt ttttcaataa
    24541 gactccacaa aaagggaaaa aagaaagtgg ctttcacgta cgtttgtcgc aaagcagaag
    24601 aaaacattaa aaataaacta aaacaacaga ttaaaaaaat tcaatacgct ggagcaaaaa
    24661 aaaataggca ccgagcaata ggtgactaca acatgatggt catcggtatc cacaattact
    24721 atcggatagc ttcacattgt agtgtcaatt tcactgagat ttatagaagt gttaggacag
    24781 taatagaagc aagactgaaa gctagaggaa tgacgaaaaa tggaatctat ggacaagatc
    24841 atgaaggata ccgtccgtat ctaaagtcta agcaaatgag atatattgaa ggttttccga
    24901 tacttccaat tggctatatt aaaaaaagaa ctccactcaa caagcggaga aatctaaata
    24961 aatataccaa ggaagggaga aagctcattc atcaagagca acaagctata gaaagctgga
    25021 aaatcaagta tcttgtaaaa ggttttagta acaataagaa atacacggtg gaattttctg
    25081 ataacaaaat atctaagttt atttcccaaa aagggaaatg cgctatcaca ggaaacgaac
    25141 ttttgatgga agaaattgaa tgccatcaca ttaacccata tcatttgtcc aaagacgata
    25201 gttacaagaa ccttgtcatt atctctagag aaatccatag attaatccat gcaactagtg
    25261 aagaaactat tctgaaactg cttcggttac tgaaactaaa tcactatcaa attagaaaac
    25321 taaacacgta tcgaaaaaaa gcagctaatt ttccaatatc aatcaaaatg aattagatag
    25381 gaaacaaatt atattgtaat taacgatgga acgccgtgtg cggtgaaagt cgcacgcacg
    25441 gtgtggaccg ggggaaaaga cggagataat atcaaactct tacctatcgg aattgccacc
    25501 aatggataat atggaaggaa tcatgacggt cacagctggc cgaaacatgt tgtgggatct
    25561 gtttattcaa tcttatcagc aactttatgc gaagtatgga gaacaaaacg cagcgattat
    25621 caaagaaaat tgtcagacac atatttatat tatgtcgaca gatgataata ccatcgaaga
    25681 attttctaaa aaagtgggga ataagacggt tgaacaagag aacagtacca tcaattcatt
    25741 aggtatgaat actcatatca atcgcaatgt tgattcagat cgtatcttaa ctcctgaaag
    25801 aatttctact tttttagaag gtgaaacgat tgtactcgct cctttaaaac gacaagattt
    25861 gaaaggtcat aaaatacggc cgtatcctat ttttaatcat ggggaaacga atcttcctta
    25921 tgcttatcaa tatattgaag atttcaatcc atcgtgggat atcaatgata tagacataca
    25981 ttgtacacat gcggacttgg atcttaaggc tttacaaatt gattatgatt atttcctacc
    26041 agatgaatat gctagaaatc tctatcatgg actgcatgat caattagaag aaaaaaacga
    26101 agaaaatatg ttttataaaa tcgttcaatc tcttgagaat gagacggcta aaaaattgtt
    26161 aattcaattt tacgaagaac aagcagcaga tcgacttcaa gaatatgttg aaaagttgat
    26221 caaaaatgaa gaaatttctg aaatgattgg gaaacggttg ttattggaat ctgaaaaggt
    26281 ggtgaaagaa tgatcattgg aggaattttt tctggcatag ccgactggtg gtcaggctta
    26341 ttcggagaag gggatgcaaa ccccaaaacc gtaacgaaag tcttacaaga ttatggtaat
    26401 tatctgcagt atcaagatgt gttcagttgg atttgtaata cttttttatg ggggttgatc
    26461 aaagggttat ataatctcaa tactttacta gaaaaaacca tttatcaaag ctttgattta
    26521 aaagacttat tgaacgccgc tggggtaaat gaactatttc aaagtttgat tagtaaggta
    26581 ttggctcttt tttgtgtcgt cacattaatt tatttaggct tgaaattttt tatgagtaaa
    26641 cacccaccaa aagtcaaaaa tatcttggtt aatttattta tggccatgat cttgattttt
    26701 ggtggtagtt ctttaatcga tgaagggtta aatatcagta aaagctttta cggagacgtc
    26761 actgcagcaa ataaagcaga taaaacgggt tcacctgctt ttcaaattat tcaaaataat
    26821 gtgatcgatg tcagtacatt actagcaact gacccgaata aagtagaatc cctgccaaca
    26881 gacaaaagaa atgctttaac agcgaaaaat tttaaaacag cgaatatcaa tgggattatt
    26941 actcctaaac aagctaaaga aatggcggat aaagataaga gtggtttatc agatttacaa
    27001 aaggatcggt taagagcgtt acaataccga ctggaactgg atgataatgg aaaagaaatt
    27061 cctgtgcaaa tcagtgatga aggcttagcc aaatatgttt atacttcagg gtatcgtcga
    27121 tacattagta gtccagggat tattttagct ggtgaatctt ctttggcggt ggcctattta
    27181 tttatcctat ttacgatcgt tacttgcatt attgagctgg tatttaaaaa attctattta
    27241 gtgattgccg cgtcaacgga ctttgagaca ggacaacgga tgaaaagtgc tatcgaagat
    27301 atttctcaat ccttcttgtt attggcattt acaattttag aattacgaat ctatacctta
    27361 atgctttcag gtattggtga tttgcatgcg gcaggaaaga tcaatggatt cttgtatgtt
    27421 gtcgctttga ttgtactaac cattgcgcta ttcaaaggct ctcaatcagt tacgaaaatt
    27481 ttcggcgttg atacgagtct gaaaaacggc agtaattctc ttcttagcat gtttgcctta
    27541 ggaaatattg cgaaaaatgt gggtagcgga gctaaaaacc ttgcaagtgc tggtaagagt
    27601 ggtttaagcc atttaaatga tattcgtaaa aatggatttg gccatcgaaa agatgttacc
    27661 cctgataacg gggaagaaca atcagacgat aaagctacaa ctgtacctaa tagtaagaaa
    27721 acagtttccg aaatgttcgg gaaagcgaaa aatggtgttt ctaaagccgc agaaggtctt
    27781 ggttacgtca atgaacgtgg gttaaaagga acagctgaag atcttacaga aaaagccaaa
    27841 gacaccttag aagataaatt agacggcacg agagctaaag aattggctga agctgtgaag
    27901 aatccatccg aagcactaga aaaacaaaaa gaaaaagctt cagaagtcaa agaaggacta
    27961 aaagatgcgt atggtgaagg acaagtcaat gcagcagtga aagctaatac aacaaccgaa
    28021 aaagaaaatc aagaaggtat agtgattcct tcagtcaaag atcaccaaga agcgttgcac
    28081 tctaatgaaa attcgattga agagaaaaat tccatagaag aaagaaattc agaagaagga
    28141 aaaaatggac agttaccaaa aactgatcca gaacaatatc gctataaacc taacagtgac
    28201 cttgaaaatg agaagcgaat ttcagattta tcaaaagtaa atcaagaaga caaagcagaa
    28261 aatgagggac agttaccaaa aactgatcca gaacaatatc ggtatagatc tgttaactcc
    28321 gaaaatgaaa aacgcatttc agatttacca aaacgaaatc aagaagtaca aacggatgag
    28381 gattccatcg ttctgccaaa tgataatgga atcgaagttg gaactgaaaa atcaacgaaa
    28441 attggtttac ctaaggcaca aaatttacca atgaaagata gtcgtttgaa tcaattcaag
    28501 gataatgatc aaagttttgt tcatcaatca cctgcaggta ctgttcaaat gaaaccaagt
    28561 cgtttgcctg aaaatggaaa tctgacaact gtttcttaca atttatctga aagtggtgag
    28621 acagggaatg cgtccgttca aaatgttgtg acaagagaag ctgcaccaaa acctattcaa
    28681 aacgttcata caggttacaa gccagcaaat gtggcattta ctgcaccaac aaaaataaat
    28741 gtgacaagag aaactaaaaa caggaacaat gcattgaaag aacaactatt gagggatatc
    28801 aaacaggata tcaaaccata aaaggcctac aagttaggcc ttttctttat atgaaaagga
    28861 gaaatcaatt atggataaat atataaacat gaaaaaccct ccaagattta gtggagaatg
    28921 gaagtttttc gcttttttct ttttagatct attaacgctt ggtggctgct ttttttgggc
    28981 gaaaatggtc aataatcttg tccacttacc actctttttt gcactcttga attatggctt
    29041 atggattgcg atagcgttag tgattgtttg gcgaccctat aataacccag gtcaacgaca
    29101 aatttttgcg attatcgagt ctattttttt tgcggacaag aaccattatc atgcgttaga
    29161 tcctaaccga aattatcatg gtacgtataa ggaggatgaa taaatgcgag tcaaaggaca
    29221 aaattcagtt gaaaggaaat ccattttgca gtttggaaaa ccagaagagc gctctatctt
    29281 ggacttattt ccgtataaat cctgtacaga agagggagtc ttaattacta gagaagatca
    29341 ttttcaacgt tattatcgtg tagtttctac agacgtggaa ggattaaatg aacaagagaa
    29401 actagaacga atgaatcagt tgaccaccgt catgcgtacg tatgtgccaa caattaagtt
    29461 agttagtttg accacagaga cggatctaac ggaacaaatc gtgcaaaagc ggcagttatt
    29521 agataaaaat cgtatggaac agtccttagg aagaaatcta cagcgattaa gaaaatatga
    29581 aaagaaatta gtagaggaaa tccaggaatt gaaacgagca gaacaggaac gccctgattt
    29641 aagcttcttt ttcgtcattg aaggaaagac aatcaaggat ttgcaaacca gaaaccgtca
    29701 gttaatgcga tctagtggca ttttagggtt aaaaccactc tacaaaaaag aattgatcaa
    29761 gattctttat cgaatgaata atatgaatga tgagtaggaa tggggaagag agaatgaaat
    29821 atccgttgat atggttagaa aaagatgttc cgaaagaaga gaccactaga aaatttcctc
    29881 acactttaag cctgggtaag ttgggaaaag gaaaatcttt taaccaagta aagaatcaaa
    29941 caagaagagg tgactgtaca tgttagaagc aattaaaaca gctttttcac cacaaaatga
    30001 tcaaaaggta cttgatccat taaagcaact aggaaatcgg gaaatagaaa ttttaaaatc
    30061 caaagggtat gatttagact ttttaaaaca agtgatgcca caaggtggat taaatttcga
    30121 tgaagagtat gtgacctatg gaaatggtta ttcacgatgt gtgacagttt atcactacac
    30181 aaaaaatcct acgctttttt ggttggccaa tctcatgaac aatcctcata cagtggcaac
    30241 actagatgtt agcacagatg aaaagcaaaa agtgattgat caaatcaatc gtgccttaga
    30301 tgaattaaat gatcggtcag ataatgaacg aaaagcgact gaccgaaata aaagcgcgga
    30361 agaataccta gaactagcgg aatatgcccg aaaattgact tctaatgggg aaatttctaa
    30421 acaagtcaaa gtgagagtac atgtttacgg aaacacgatt caagaagtag atgaacgagc
    30481 acaggaactg atcgaaaatc tcaaagggat ggattatcgt gcagtgacct atctgcacaa
    30541 aacgaaaaga gactggcaat cgatgttcac catgttcgat gaacaagctg aatggtttgg
    30601 aaatatcaaa gggcaatcac taccatcgct aaacattggc ggaggtttcc cgttccacca
    30661 ccaaacgcta aaagatccag gtggaacaca atatggggaa accatgacag gcggcccgtt
    30721 tgtttggaat gccacgtata aaaatagtgt gcggttgtct accaatacct tgcttctagg
    30781 aatgatggga gctggtaaat cgaccgttct taaaatgatc gcggaagccc atttagctgc
    30841 aggagacttt atttggggct ttgaaaaggg aaaagacttt attccctttt taaaggaata
    30901 taacggcatt atggtgcgtt tagacggatc tgacgggatg atcaatcctt tagaaatatt
    30961 tgccacacgt acgtatgatg aagcttccag tttatatgac gatggatcag tcaaagattt
    31021 gaagatcaat gaagcagcgt cctatcaaac gcacttagac aaagtggtct atcaggtaca
    31081 gttagtgagt cctcagctaa aaggaacgat gaaagcagaa tttaagacct atttaaatcg
    31141 tttttatgaa gagtatggaa cagtaccacg cggctttact tcctcaaatt cgcgttcaaa
    31201 tactgatacg caagtcacag gtaaagatcc agaagcttat ccgacgttca aagaattttt
    31261 agacttttta ggacaactgg aattaccagg tgcttcccaa gagaaaaaga atcgaaaaga
    31321 agaaatggaa tccatcgtag aatccttatg tgagacgtat ggcatgattt ttgatggtca
    31381 tagtacgatt cgacaccttg accaacaaca gttggtcttt tttgacattg actctattac
    31441 aggcttagat cgaggggtac aacaatgtca aatgtacatg gctatgacgt tgatttggaa
    31501 tcaggcattg attcaaggaa gaagacaacg acgtttaatt aaagaaaaac aaatggaaga
    31561 acaagacttc aaatgcttct tggcgatgat tgatgagtgc cataatggtg cgacctgttc
    31621 tgcagtgaaa agctgtgggt tgcaagtagt ctaaaaacta ttgtaataac tcttataacg
    31681 agaagtcaaa tgagtgggta acgccacaaa gggcttccac tgatactccg acatgcaaca
    31741 agtagtttat gcagaccaac gttgtgtgaa gctcggtgaa gtcggctgaa ggataccgta
    31801 aggtaaaaga aatagtgaat accacgaaag ttctctagag gtaaagggtg tatccgataa
    31861 gtcgggtgtc caaaaaaact taatatggtg agtatgtaca aacgtactga cgaactagcg
    31921 aatgtactgt ccgaaaggca tagctcctag aaatgggaga gacagtgagc aaaaaatcac
    31981 tcattgtgct aacttgtggt tgagtaaaag tcgttgttat gaaagaccat gctgcgttac
    32041 aggcgtagga gaagttaggg ggcaaaagct agcatctaag ttaaggcatg tggatagata
    32101 taagggaaca ggggaagcca tcaacatgga gagtgcatac tcgatgaagt ggtagtaaga
    32161 aatatgtgaa tgacaaataa cttactttag tgatggtgaa agtagagtca cgactgatga
    32221 aggttctgta atggaactgg aggaacaggc tcgattcgtc tgtaaaagtc atgaaacccg
    32281 ttcaaccatg ggattctcgt atgactaaaa aggtcacgag ccaattggag cgtgataaac
    32341 catgacaaaa gaaacaagtc caaaagatag gaagttgcgg catgcagaat attatggcat
    32401 gacggaaatc ttcgataaga tatactccaa aagtttgaag ggagataatt tcaagaatct
    32461 aatgcagatt atcatttctg ataacaacat tgtcctagca tatcgtaata tcaagagaaa
    32521 tcacggtagt gtgacaagag gagaagatgg agtaacgatt aaagatattg aaaatctcaa
    32581 ccgtaatgaa ttcgtagaaa ttgttaggaa gcgattcgca cattataacc ctcggaaagt
    32641 aaggcgtgta gaaattccta aatataatgg taaattaaga ccgttgggta ttccatcaat
    32701 atgggataga atcgcgcagc aatgcatctt acaagtatta gaaccaatat gtgaagcaaa
    32761 attcaataat cacagctatg gtttccgccc aaatagatca gcagaacacg caatagcaga
    32821 ttgtatggcc cgaatgaatc aaagtcacat ggaatatgtg gtagatgtag atatcaaagg
    32881 tttcttcgac gaagtcaatc atactaaact catgagacaa atctggtcta tgggtatacg
    32941 agacaaacag cttctcttaa tcattaggaa aatgttaaga gcacccgtag tacttccaaa
    33001 tgggaagttg atatacccaa caaaaggaac accacaaggc ggaatactta gcccattact
    33061 tgcgaacatc aatctcaacg aatttgactg gtggatagca aatcagtggg aggaaaaaga
    33121 tgccaaagag ctaaaacatc attttaggaa aaatggagct tgggataaag gtaatatcta
    33181 tcacaagctc agaaagagta caactctaaa agaaatatac ctagtgagat acgcagatga
    33241 ttttaaaatt ttctgtagaa attataatga agcgaaaaaa gttttctatg caactaaaat
    33301 gtggttacag gagcgtctaa agttaccaat atctgaagaa aagtcaaaaa tcacaaatct
    33361 ttcaaaagaa gaaagtgaat ttttaggctt tacgatgaaa ttagtgacca aaggtaacaa
    33421 aaaggttgtt aatacgcata taggtacaaa agcacaaagc atgattaaag tgaaactaaa
    33481 aaaacaaatc aaacgaattc aaaaaagtcc taatagcaaa gataccttta tctatattgg
    33541 agtctataac agtatggtta ttgggattca taattacttc aaaattgcga ctcattgtag
    33601 tgtagcaatg cgaaaaattg caagagaact cagaccaagt tttgaaatac gactacgaga
    33661 acaaggaatc tcaaaagaag gacaatacgg aaagtacgaa ggatacaagc cttacctacg
    33721 gtcaggacaa atacgatata taagagggtt tccaatatta ccagtagggt atattcaaca
    33781 cagacatccg atgtgtaaaa aaagaaatat taataaatac accgaagaag gtagggaagc
    33841 cattcataag aagcaacaaa ccgtagaaga atggaagata tcgtggttgt gtagcaatcc
    33901 agtgcttagt aagagagcaa cgattgaatt tgcggacaat cgaatttcaa aattcatcgc
    33961 tcaaaaggga agatgtgcag taacaggtga ggagcttatc ttatctgaaa tgcattgtca
    34021 tcatattatc ccttatcatg aaagcaaaag tgatagctat gaaaacctag tgattgttac
    34081 tgaggaggtt catcgggtaa tacacgctac acaaagtgaa acgattgagg aacttctaaa
    34141 atatctaaaa ttaaatccaa aacaaaaaga aaaactcaat gagttgagat taaaagttgg
    34201 taacgaagaa atatcttaat aaatatttgt aggacgggaa aacagaatgt cttttataga
    34261 cgagggaacg ccgtatgcgg tgaaagtcgc acgtacggtg tggactgggg gaaaagacgg
    34321 agataatatc aaactcttac ctatcagtat cgattaatcc agattttatt gaaacggtaa
    34381 attatgtcac aaactttcaa cgagaaatgc gtaaagtaga agcaatgact gttttagcta
    34441 cacaatcccc acaagaaatg gtgccagaaa atatgtcttc tgataacttc tcgaaactga
    34501 agaaagtctt tgaattttct tctacgaaga tctttatgcg tatggatgaa tctattttac
    34561 gtcatataga aggattagtt ggtaaatcaa tgacaaactc agaattagaa tccattcctc
    34621 aacaaaatca aggagacgct gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt
    34681 ttagtccgaa caaacgtcaa ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa
    34741 tgaaagttta atcaagcagt atgttaagcg tcgtgcaaag cgacgcttat ttttgtggtt
    34801 gtttggtact tctgcaggtt tgattactat tttgattact gtgttcgtga ccctttttct
    34861 aatacttgcg gcgggttcga ttgataattc tgattcagat tcaagtagtg gtggcgaagc
    34921 ttttacagga gaatattcgg aaggcttacc gatttataaa gaaatcaaag gaagaggccc
    34981 tttttctgat gagattgcgc aatatgctgt aggtgcagca gtgaaatata aattattacc
    35041 aagtgtgatc ctttcgcaat acggttatga atcagcgttt ggtacgtcgg cgtccgcaag
    35101 aaatgatctc aactattttg ggattacttg gtttgatggt tgtctgtttc ctaaaggaac
    35161 tgcgcgagga atcggtggta ttgaaggtgg ctggtacatg aagtttccca actcaaaagc
    35221 agccttctcc tattacggct ttatggtagc cacacaatcc aattttaatg catgtgtagg
    35281 aaataaaagc cctggcgcaa gcttattgat cttaggacga ggtggttatg ccgctgcagg
    35341 aattactgaa gatagtccgt attatacagg ttgtatgtcc attatcactt ctaataaatt
    35401 aaccgaatat gatgaattcg ccatcaaaca ttggggtgaa ggtggcaata acaacggcac
    35461 gatcactggt gaatggacga atccattccc tggcagcagt ttagataaaa gttccttcag
    35521 tggtggtcaa ttatttggaa caaatccagg cggtgaattc cgtccgaatg gcttccatga
    35581 tggattagac tttggttcag tcgatcatcc aggaagtgaa attcacgcgg tgcatggagg
    35641 aaaagtcgtg tacgttggca atcctggtat ttcaggttta ggtgcatgtg tgatcgtgat
    35701 taattacgac ggtttgaata tggtatatca agaatttgcg aatagtacag gaaattctcg
    35761 tgtgaaggtt ggcgatcaag tgaaagttgg tcaagtaatc gggatacgag atacagcaca
    35821 cttgcattta ggtttcactc gaatggattg gcgtcaagca caaggacacg cattcacaga
    35881 cgatggtaca tggattgatc cattaccatc tttgaatagc agcaaaaaat agatcaggag
    35941 aaaaaatatg gaaaataaaa ctttaaaaat cgtcttaaca gctgcaatta gcgttagttt
    36001 aatcggcaat atcgcgttgt tttggcagct gaaagaagaa caagagacaa atacctttaa
    36061 aattgaagaa gtggctaaaa acgcgtctga gaagctcaaa aaaaattcct ctgaggcaca
    36121 ggcgaagatc gacaagttaa atcaaaccat caatgagaaa aaccaagaaa tcgatcagct
    36181 gaagcaacaa gatggcacaa aagcaaaaga ggaagtaact gaagcacaaa gaaagacggc
    36241 agaagagttc ggtcaattgg ccattgataa atcattaagt agaaatgaat taacggaaaa
    36301 actaaaagat gtcgcaaccc aagaagtgat cgacaaatta agtcctgcag acgatgatca
    36361 ccaacatgat gattacggaa gttactcctt tacattaggt gacgtgcaaa cttatgctca
    36421 aacaagcagt gtaaaagagg aacaaaattt tgtcgtattt gttgattata caatcggcaa
    36481 tccgcaattc aaagatgtca aaccacaaaa aataaaaggt ggattaactg tcactgaaaa
    36541 acaagtagat ggtcaatgga aagtcataga ctttagttat ttcactcgtt aggaggaaga
    36601 aaatgagtaa gaaaaaagga gaaagacaac gtttcaaaaa acgaaaaagt tatcttcaat
    36661 ggctgggaat ctttatgatt ctcggctttt tgattcttgc tagtccgatt tatacgaatc
    36721 agaaggtaga tataccagat gaggtaagca cgaacgcctt tttaaacacg aaagagctga
    36781 caatggtttc aaaagaaaaa ggagcgaacc agcaattagt ttttgaattt gcgatcgatg
    36841 atgaagatca aagcttgtta aaagaactgg ctaatcttaa ttatcgcgta gaagtaaaga
    36901 cagctaaagg ggattatcaa gcaatacgag ccaaagtgat caaggtgtct gatgattatt
    36961 ttgcagtgaa gctaacgcat gtaccagaag aatatatcgc catgcgttta accattattc
    37021 ctgaaaaaat tgatccaaaa gtggatatgc aagaaccaca agatctgatt tattacgtgc
    37081 acgaagataa ggttaaagat caagtgaaag atgaagatta tgaacaacac gcgatcaatt
    37141 acaaagtgaa aggatacaag aaagaacaaa aagcagctca aaaacaaatg gaatctttcc
    37201 aagcaaccat tgatttgaat gaagaattag tgaagaagct aaaagatcaa ttgccttatc
    37261 aagtcgcaga tgatcaggaa aatactgaaa acaagattaa tagctaccag caagaaattg
    37321 aaacatcgaa aacgcaactg aaagatcaag aagagatcat tcaaaaactt caagaaaaaa
    37381 ttgatcgtat gacaaaagaa aagatttaga aggacaggtg gagtgaaaaa atgaagcgaa
    37441 caaacttggc tgacaaagat actttcgctg agcagaaagt attgattaaa gcctatctca
    37501 aagttgtaaa agctaaagaa atatgtcgag aagtgaattt atctaacaca acaatatctg
    37561 agttgaagaa tggtcgaaag aatttagata ctttaaaatt cagatcattt gaagctctat
    37621 atgtagctgc taaaaaatat tttacagctg ataacaaata aaaaacagga gtattttatg
    37681 tgaaaaaagt atataaaaaa tgcttatgaa tcagctatat caccttaacc atttgttaat
    37741 gagatgggag aaaacattaa ccacgattct aacgcaaaca tcaagcacat aaaacattta
    37801 acactcctcc tttctggctg aagatttcaa ccaaacagga agacagcgta tcaattatag
    37861 catacacttt ttcttaaatt catttttcat atgacaaaat atttccttct aatcgacaaa
    37921 gttagaagga ttttttattg tctaaataca caaataacct taatttttaa agagcataag
    37981 gaaacgtgat atcactttct cacgaaaggt gttaggtagg ctgtgcctac cacgtttcta
    38041 agaaagataa atgatatcaa aattttggat ttgtgtgtag atttgtgggt aggatgaaag
    38101 cgaatgtggg cagtaaaata agcgtttgtg tgtagcaaaa tcaaaaaatg tgtagggata
    38161 tgtgtagcta cacatatccc tacacataat aaatttggag gaaaatatgg acttattaat
    38221 tagaaatatt gaagaaaagt atattaataa aattgacaaa cgttgtgaag aacttacggt
    38281 caagactggc aaaaaatgga gtcgaaatca atacctaaaa gttttgatcg aaaatgattt
    38341 tgatcacgct cttctgaatt ataaaaaaga ccaatttgat cggttgttag aaaaatttgt
    38401 ggatattcaa acttataata cgaaaacttt agaagaatat attgcaacaa ataatcaatt
    38461 aattggtttg ttaattgaat aataaaaata gtattgtgaa tttataaaag gaggaaatca
    38521 attttggcaa aagcaacaag aaaacatgta tatctttacg atatgcatat cgaagtactt
    38581 gatgaaataa tttctactca tgaagatata tctaattata gtgaagcagt cagacatttg
    38641 tgtatgatgt atgatccaaa aatcgaagaa aaaataaatc caattcaaat taaattgaat
    38701 gcaatgggaa aagaaatttc tattttaacc gaaatggttt cagagtttgc ggatcttcat
    38761 ttgaaagata caggaatact aattggtaca aattcaactg tttatcaaga agcagaagaa
    38821 cgtgtggaag gaaaaattaa atccaatcaa accaaaaaat attcttctaa gttaaagaaa
    38881 ccagtgaatg aatctgttca taccagagta gatccatttg atccaacgaa gattctaaaa
    38941 agataaaagt agaaggagaa cgtgatgaat aaatcgcctg cagttattct tacttgtcaa
    39001 tttaccttgc ccaataaaaa atctttttca acatacattg attatatgac aagagaaaaa
    39061 gcactggaag aaatggaacg acgtacgccc gaagaagaac aggaactgca acgaatcaag
    39121 aatgctttag aggattttta tattcctgaa ggagaaactt tttcggaaag taagacggat
    39181 aaagaattca gtgaaacgtc gaatgaagct caatcgttga tggaacatgg aatcaatttt
    39241 gacaagttag aggaacaaga ttttaccaaa tatctttctt atatgacccg tcactatgcg
    39301 ttagaacaaa agaaagaact gacgcaaagg gaacaaaaag aataccaacg gttgaaccaa
    39361 gcaatgaaaa aatatcaaac agagccacat aaaaaagctt caggaaatct tcctggagtt
    39421 ttttctatgg cttcagacga agtgaaagga agtgatctga aggagatcaa acaaatcttc
    39481 caaagaggac aacaaaaagg ctcaatcatt tatcaagatg ttatctctca cgacaattcg
    39541 tatcttgaaa aaatgggctt gtacgatccc aaaacagata ccttagatga agaaggtttg
    39601 aaagcagctg gaagaaaaat gatggagaca ttattcgaga aagaacaatt gaatgaaaca
    39661 ggttactgga tggcaacgat tcaccgtaac actaagcata tccatattca ttttgcagtt
    39721 gttgaaaaag aaaatacgcg taaaccgtat actgagattg aaaatggtgt gagttatatc
    39781 gtaccgaagg ggaagagaag acaagcaaca ttagacgcaa tgaaaaccaa ttatgtacat
    39841 gagttagaac gtttctctta tgaaaaggat cgaatgatcg gtatggagaa acgaaattta
    39901 ttgacacgaa aaagtgattt gcgtaacacc ttaacaaaag tagtaaaaga accagcacgt
    39961 tacgatcgac acgccatgaa cttattaaat gaagtatata aaaccctacc tgaaaaacgt
    40021 tcggaatgga attatggaga tgaaaatcga accaagcttt caccatttac aaggggaaaa
    40081 cttgatgagt taaccaaata tgttttaaca aaagaacctg gatatcaaga atatatacag
    40141 atttcaaaag cattgaaaga agaagccaaa agcctttatg gggaaagcaa aagagaaaca
    40201 aagaatgcag aaaaaaacgc cctctttgat ttaaaaaagc gcacaggaaa tgcgatttta
    40261 acagaactga agagacagga tagtcaaata aaaccattaa tagaaagtgt caatcagatg
    40321 caatctattt acaatgaacg atcctttcga acactcaaac agttgccaac aaatgaatac
    40381 aagtatagtt atcaagaatt cgtaaagcgt aggaaacaat tccaacaaag agttgtcacg
    40441 cagcgaagct tgcgtcaatt aaatcgaaca attaatgcac atgaagaaaa ataccacgca
    40501 agtaaagcat atgaagagca acagcgaaga atccaaatgg aacaagaaag acaataggag
    40561 gaagagaaat gaaacaatat ttagatcaat ggaaagtcat agaaggatca ctaaaagaag
    40621 aacgaatcga acaacttcct gattgtttag aaaaagagca tttgtttcag ataagcgaaa
    40681 tgttgagaaa cgaacagttt gatccaaatc aatttttagt tgttgagtac ccagctactg
    40741 gtgtgtattg ttgtaatcat gtgaagggtg aaaaatattt tattattcaa gaatacgaag
    40801 gtaagttagc gccttattat actacttggg aaataaatga agagggaatc aataatttcc
    40861 catgtgaaag cattgaagag tctatttctc tcacggaatg ttaaataaat agagacgtaa
    40921 aagagtcctg aacgtgattg tccaggactc tttttcaatg aattgttttc cgccaattca
    40981 ctgagtggtg cgattcaatt ttattatatc aaaagaaagt gagattccaa agtgaaggga
    41041 ggaagaagaa atgactcaga caacgattgg tgttgtggtt gaaaaaagca gggataatct
    41101 cgtttttgtg acagaaattc aaacaggtag agcttttgta gtcacagaca aagctgcaaa
    41161 agcgtatcaa aatggcaata ttttaacgtt aaatctgact acgaagatgt tcgttgatgc
    41221 agtagaaaat tatccatttg tttagacaaa cgatatttta caaggaggaa acggacatgg
    41281 cagaagaaaa agaaccacta gataatagtc gtttgggaaa gtccaaaaga aaattagtac
    41341 ggttgcaaaa cgaattaaat aatcaatttc aaaagctatt tgatcaccaa aaattgacaa
    41401 atgggcaacc aatgaatgac aaacgaaatg gtcgttcatg gtttaaccag caagaaaaaa
    41461 tagaaaataa aatacaaagt ttgagagaag agatcaagca acaagaaaaa caggtagaaa
    41521 aacttgaaag acaagaagag ctaaaggaaa tgggttataa caagtacggt ggcctggaca
    41581 tgacgatcga aaatattcca agaatcaaag aagaaatcga acgctttgaa aaaggtgaat
    41641 cgactttttc agctgccacg atccgaaaat accaacgaaa attggaaaca ttagaacagc
    41701 tgaaagaaaa atcagagaaa ggaaaagaaa atcttcttcc tgaagtacaa gcgatcattg
    41761 acagcggacg agtgacgcaa tggaaaaaga atccaactat ctattttctg aaaggctatc
    41821 gaaaagtcgc tttagaactc aatgaaaacg ggggatttga ggaatctaaa aaataccaag
    41881 ccacgtcgga agaagaaaag gcagctgttc gtgaattact tgaagaagct acacctactg
    41941 attctgtgaa ggagaaagaa accgatcatt tctatcgagt agaattcaac gaaaatgatc
    42001 cagcaaaagg gttgaaaagt tatggtgggg agatcgtaac actagaattg atcgatcaac
    42061 tcaaaacact ggataaccaa tacgccgcag aagcaggcta ttataaattc tattttgaag
    42121 aagtgaagaa tggagaagta gtcgatcaga tacgaatgga tctaggggat ggcgtcaata
    42181 gtaatcaccc catctattta gagttagcta gattgtgcga acaatcacct gaaaaggaac
    42241 aagaacaaac agtcaaaaat gaacctattt caaaagtaca aaacgagatt aaagagacaa
    42301 aaacaaaaca aaagggttat caaaaacgaa cggcagaaga gattaagcaa gaagtcaaat
    42361 cactttccca acaggcgctt gatgcggtga aaaagcatac tcattcacct gaagacgtca
    42421 aagaactttt gaattttatg tctcgttttc cagaacgatc ctttagaaat caattattga
    42481 tcgaggaaca atttccaggc gcaacggctt gtttaggtcg tgccgcgttg aaaaaagaag
    42541 gaatctatat caaaaaagga gaacaaggga acaagatctt tgtcagaaaa acggtcaaag
    42601 gattttatga gaaaggacgt ggctttgttc gagaaacaga ggctacacca gaagataaaa
    42661 aacgaatcga aagtggtcaa atcgaagtga tcaaaaaacc ttactacacg attgaaaaag
    42721 tgtttgatat cacacaaaca caattgaaac ctgaagacta tccgaaaatc ttcccgaacc
    42781 gcgtctttga ttttcaattg gatcaaacag ggcagacaga acttcaagtt gggattcaag
    42841 cggtagccaa aaatattgga attcaaattc gagatatgac agaaagtgaa gtctatcaaa
    42901 gagaattagg acaagcacgt ggggcgtacg ttcgtaacga atttactggc gaagaagaaa
    42961 tcgtcatgaa tactcgaaat actcctacgc agcatttagc cacaagtatt cacgaattag
    43021 cccatgctag aatgcacaaa ttttcagaat tagatactgc aacaaaagaa ctacaggcag
    43081 aaatgacctc ctatattgtg tgcaagcatt ttgggatgga cacctcggag aaagctattc
    43141 cttatattgc gacatggacg aagaatagtc agacgttgga tgataaggaa gctgcggaac
    43201 gtgggaaaat catgaatgat gtcagtcgtg tcgcaaatga attcattcaa accatttcta
    43261 atgaaatcaa tcaatacaga gaaagagaac cagagatcca gccagcagaa gtgaagcagg
    43321 agcataaaaa tactgtagaa acggcagaac aaaagaatat tgtgcccgtt ggctcacttt
    43381 ggatcgacaa gaaagacggg aaagtcctgg aagaagacac gggccgaacg ttacatttga
    43441 aggatgcggc gtttattgaa gatccgcagc ataatcaagt gattttaaaa ggaaaaagtt
    43501 atggaaaaga gatccaaacg gcgttacctt cagaagaatt tgcgctagga aatccattcc
    43561 gattttcact tgagacagtg tcagaagtgg caaaacgaaa aaatgaaatc tattatgaac
    43621 aacaattgtc acaaaaagaa cgcgtataaa acaagcaaga aagactatct gaaaaatgga
    43681 tagtcttttt tcttttggga ggtttgaaaa tgacagagaa aaaaatgagt ctcattgatc
    43741 gatgcaagca aatggatatt gtggattttg cacgaaacaa tggtttagcg gtggccaata
    43801 aaggacgaga ctatcgctta gaagaccatg attcatttgt ttttgatcga agaaaacaac
    43861 gtttttattg gaacagtcaa aatattagtg gcgacattat tgaattagct aaactttttt
    43921 tcatagacaa agagatccaa gaccctaagc aacaatttaa agcagccact gattttattc
    43981 taaagaatga agacaaaaca gaacgagtgg aaaatcttca ttttgagaca gaaaagtaca
    44041 aagatcaccc cgttgattat caaccactca cagaaaaagg aaggaattat ctgaaggaag
    44101 aacgaaaact gcccgattgg ttgattgatt atgcagaaaa agaaggattg attgccgaac
    44161 taaagccaaa acatgaacgg caaaatttct tagttcgaga cgatcgtttg gatcatgcgg
    44221 tcgccttttt atggaaagat ccacagacaa aagagacagt aggggcgagt tatcaaggaa
    44281 cgttcattga ttatgaacgt tttggtgaga gaggcacata taagcatata gataaaaatt
    44341 cgacagcgaa tcatggcttt aacctaaaga ttggtgatcc taaacagtta aaattttttg
    44401 agagtagtat tgatttactt agctacgctg cactgaatcg tgatcagtta aacgatacgt
    44461 ggttagtctc tatggaaggg ttaaaacacc atgtgatcag tcattatttt ggtgaggcag
    44521 tatctgaatt gagaaagaaa caagcctttc ctcaatccat cgagatttgt gtggataatg
    44581 accgtgcagg acatattttc tacgaaaaag aacaattaat gggcgcagtt gatccattta
    44641 cgaatcagaa agtacgttgt gaacgaggaa tcgctaatga ttggcaagtg ccaagagaat
    44701 acaaagtcat ttatgaagaa gtcgcaaaag aagaaaaggt cacaccagaa gctattatgg
    44761 ccatacacaa gacagaaaac aatctgcaat taacgaacca actggtttcc gctcacaaag
    44821 tcaatgcgtc ttttggtcag cagcttt
//
LOCUS       DACDTO010000015        42089 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDTO010000015 DACDTO010000000
VERSION     DACDTO010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.015"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            347..1957
                     /locus_tag="IWK70_000862"
     CDS             347..1957
                     /locus_tag="IWK70_000862"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9613428.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            2249..3118
                     /locus_tag="IWK70_000863"
     CDS             2249..3118
                     /locus_tag="IWK70_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9613429.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            3283..4554
                     /locus_tag="IWK70_000864"
     CDS             3283..4554
                     /locus_tag="IWK70_000864"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9613430.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            4551..5846
                     /locus_tag="IWK70_000865"
     CDS             4551..5846
                     /locus_tag="IWK70_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9613431.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            5945..6208
                     /locus_tag="IWK70_000866"
     CDS             5945..6208
                     /locus_tag="IWK70_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9613432.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            6653..7102
                     /locus_tag="IWK70_000867"
     CDS             6653..7102
                     /locus_tag="IWK70_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9613433.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            7159..8577
                     /locus_tag="IWK70_000868"
     CDS             7159..8577
                     /locus_tag="IWK70_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9613434.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            8577..9437
                     /locus_tag="IWK70_000869"
     CDS             8577..9437
                     /locus_tag="IWK70_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9613435.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            9611..10384
                     /locus_tag="IWK70_000870"
     CDS             9611..10384
                     /locus_tag="IWK70_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9613436.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            10531..10896
                     /locus_tag="IWK70_000871"
     CDS             10531..10896
                     /locus_tag="IWK70_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9613437.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(11044..12252)
                     /locus_tag="IWK70_000872"
     CDS             complement(11044..12252)
                     /locus_tag="IWK70_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9613438.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(12271..12549)
                     /locus_tag="IWK70_000873"
     CDS             complement(12271..12549)
                     /locus_tag="IWK70_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613439.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            12742..13398
                     /locus_tag="IWK70_000874"
     CDS             12742..13398
                     /locus_tag="IWK70_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9613440.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(13840..14637)
                     /locus_tag="IWK70_000875"
     CDS             complement(13840..14637)
                     /locus_tag="IWK70_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9613441.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(14634..15818)
                     /locus_tag="IWK70_000876"
     CDS             complement(14634..15818)
                     /locus_tag="IWK70_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9613442.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(15843..17090)
                     /locus_tag="IWK70_000877"
     CDS             complement(15843..17090)
                     /locus_tag="IWK70_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9613443.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            17305..19050
                     /gene="dnaX"
                     /locus_tag="IWK70_000878"
     CDS             17305..19050
                     /gene="dnaX"
                     /locus_tag="IWK70_000878"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9613444.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            19070..19384
                     /locus_tag="IWK70_000879"
     CDS             19070..19384
                     /locus_tag="IWK70_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9613445.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            19409..20005
                     /gene="recR"
                     /locus_tag="IWK70_000880"
     CDS             19409..20005
                     /gene="recR"
                     /locus_tag="IWK70_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9613446.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            20069..20713
                     /locus_tag="IWK70_000881"
     CDS             20069..20713
                     /locus_tag="IWK70_000881"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9613447.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            20728..21057
                     /locus_tag="IWK70_000882"
     CDS             20728..21057
                     /locus_tag="IWK70_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613448.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            21071..22009
                     /gene="holB"
                     /locus_tag="IWK70_000883"
     CDS             21071..22009
                     /gene="holB"
                     /locus_tag="IWK70_000883"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9613449.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            22045..22869
                     /locus_tag="IWK70_000884"
     CDS             22045..22869
                     /locus_tag="IWK70_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9613450.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            22862..23209
                     /locus_tag="IWK70_000885"
     CDS             22862..23209
                     /locus_tag="IWK70_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9613451.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            23278..24150
                     /gene="rsmI"
                     /locus_tag="IWK70_000886"
     CDS             23278..24150
                     /gene="rsmI"
                     /locus_tag="IWK70_000886"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9613452.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            24259..25380
                     /gene="dinB"
                     /locus_tag="IWK70_000887"
     CDS             24259..25380
                     /gene="dinB"
                     /locus_tag="IWK70_000887"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9613453.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(25434..26036)
                     /gene="nrdG"
                     /locus_tag="IWK70_000888"
     CDS             complement(25434..26036)
                     /gene="nrdG"
                     /locus_tag="IWK70_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9613454.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(26351..28540)
                     /gene="nrdD"
                     /locus_tag="IWK70_000889"
     CDS             complement(26351..28540)
                     /gene="nrdD"
                     /locus_tag="IWK70_000889"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9613455.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            28866..29633
                     /locus_tag="IWK70_000890"
     CDS             28866..29633
                     /locus_tag="IWK70_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613456.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            29623..31665
                     /locus_tag="IWK70_000891"
     CDS             29623..31665
                     /locus_tag="IWK70_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613457.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            31946..32095
                     /locus_tag="IWK70_000892"
     CDS             31946..32095
                     /locus_tag="IWK70_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9613458.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            32106..33620
                     /gene="dltA"
                     /locus_tag="IWK70_000893"
     CDS             32106..33620
                     /gene="dltA"
                     /locus_tag="IWK70_000893"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9613459.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            33617..34822
                     /gene="dltB"
                     /locus_tag="IWK70_000894"
     CDS             33617..34822
                     /gene="dltB"
                     /locus_tag="IWK70_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9613460.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            34867..35100
                     /gene="dltC"
                     /locus_tag="IWK70_000895"
     CDS             34867..35100
                     /gene="dltC"
                     /locus_tag="IWK70_000895"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9613461.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            35103..36371
                     /gene="dltD"
                     /locus_tag="IWK70_000896"
     CDS             35103..36371
                     /gene="dltD"
                     /locus_tag="IWK70_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9613462.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            36512..37594
                     /locus_tag="IWK70_000897"
     CDS             36512..37594
                     /locus_tag="IWK70_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9613463.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            37594..38397
                     /locus_tag="IWK70_000898"
     CDS             37594..38397
                     /locus_tag="IWK70_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9613464.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            38467..39207
                     /locus_tag="IWK70_000899"
     CDS             38467..39207
                     /locus_tag="IWK70_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9613465.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            39218..40222
                     /locus_tag="IWK70_000900"
     CDS             39218..40222
                     /locus_tag="IWK70_000900"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9613466.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(40267..42030)
                     /locus_tag="IWK70_000901"
     CDS             complement(40267..42030)
                     /locus_tag="IWK70_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613467.1"
                     /translation="MLFLTGIAYFFYFIFSPADSNQMKEIKDSWVFYTKDDPDFKFDS
                     KYTRTIPTVDKDETFIMETTLEKPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEES
                     NPGLSLAVIDLPANYIGKKLTLTVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQV
                     LTMIIAVFIAIGTFFYTIYLMYKQKRLEYSLILLSCFALALGFEAVSEDILSGILFEP
                     FVHSFLSHLFIILTSAFIICYYWSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAEL
                     PELMPIANIASVISTLVTSLACIGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGI
                     YQTTINWSTILYIIILIVIISYNLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQH
                     IQEVNTLRLEFVQEMENLQDLMHTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLI
                     LARYQKLAAKRNIQITFQADLPEELPINDDDLAQLLIHALEHSFRETHAIENPLYRKI
                     YLSIHEQEEHFVICCEHSAHYDTNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQ
                     EKDTFIDRITVQLSRNYPNPV"
BASE COUNT        13527 a         7378 c         8738 g        12446 t
ORIGIN      
        1 tatttcactt aaaatttttc agtaacgatt ttctgatttg cttaggttta acgttagcta
       61 tgtttatatc aagcttatta tgtgggttaa attttttttg cactcataaa ggacttaaga
      121 aataatttct caaaggatat cgaaaaagaa aaaattgtaa gcggatagta ttcatttgag
      181 tacttgactt tcccttagtc agcttgtatt atacaccttg ggcacccttt tagagggtca
      241 gaagtttaca gaaatttcgg ctccctattc ttattgaaga atagggagct ttgtttattg
      301 tctgagactt ttcctgcaca taaattaaaa aggagttttt gaacttatga caaaatatat
      361 ttttgtgacc ggcggtgtcg tttcatcaat cggaaaaggg attgttgcag cttctttagg
      421 acgcttattg aaaaaccgcg gcttaaaagt aactatccaa aaatttgatc catacatcaa
      481 cgtggatccg gggacgatga gcccatatca gcacggagaa gtattcgtaa cagatgatgg
      541 tgctgaaaca gatttggatc tgggccacta cgaacgtttt attgacatca atttgaacaa
      601 atactccaac gtgacgacag gaaaaatcta ttctgaagtt ttgcgtaaag aacgtaaggg
      661 cgaatacttg ggagcaactg ttcaagttat tcctcatatt acaaatgaga tcaaagaaaa
      721 aatcatgcga gcagctaaaa tgactgattc agatgtgatc attacagaag tagggggaac
      781 agtaggggat atagaatctt tgccattcct tgaagcactt cgtcaaatga aagcagatgt
      841 cggtacagat aatgttatgt atatccatac aacattgatt ccttacttga aagctgctgg
      901 agaaatgaaa actaaaccaa cgcagcacag tgtaaaagag ctacgtggtt taggtatcca
      961 accgaatatt ttggtggtac gtacagagca acctgtttct caaagcgtga aaaacaaatt
     1021 ggcacaattt tgtgatgtcg aaccagaagc agttatcgag tcgccagatg tagatacact
     1081 ttactccatc ccattagttt tacaagcaca aggaatggat caaattgttt gcgaccattt
     1141 aaaactagat acaccagaag cagacatgac agaatggaaa gaattagaag aacgtgtctt
     1201 gaacttgaag aaaaaagtac ggattgcgct tgtcggtaaa tatgtcgagt taccagatgc
     1261 atatatctca gtagttgaag cattgaaaca ttctggtttc gcctataatt cagacatcga
     1321 gatcgattgg atcaaagcac aagaattaac gagagaaaat gtagaagaac gtttgaaaga
     1381 cgcagatgga atcttggttc ctggtggatt tggtgatcgc ggagtagaag ggaaaatcga
     1441 agcgattcgt tatgctcgcg aaaatgacgt tccgttttta ggtatttgtc ttggtatgca
     1501 gatggcttgt gtcgaatttg cacgtaacgt cgttggatta gaagatgcgt catcagctga
     1561 aacgaatcca gatacagcaa acaatattat tgatttaatg gctgatcagg aaaacatcga
     1621 gaatttgggt ggaactttac gccttggatt atacccatgt aaaatcaaaa aaggaacgaa
     1681 gacggctgaa gcatatgatg gagccgatgt tgtgcaagaa cgtcaccgtc accgctatga
     1741 gttcaataac aaatatcgtc aattgtttga agaacaaggg ctagtctttt caggtgtctc
     1801 accagataac cgtttagtag aaatcgtaga gctgccagag aaaaaattct ttgtgggatg
     1861 ccaattccac ccagaattga tttctcgtcc aaatcgcccg caaaaattaa tcaaagcatt
     1921 tgtcggagca tcattagcag atcacgaaac aaaataattt ttgaacttga tagaaagaga
     1981 aaagtagcaa gagaagggga taagcaattt tcttgtcact tttctctttt tctgatttgt
     2041 tattatttgt aggaaaaaag atagaaagca aaagactttt ttcgattaaa gcgctattgg
     2101 atattttatt ttttgcaaaa aacttcttga aaattttcat tttcattgat tttagtagaa
     2161 aactaccgaa atgtttggta aaatagggtc gttggttaag aattgtctta acaaatttag
     2221 ttttacaaaa cttaggagga attttattat gccagtagta tcaggagctg aatttttaaa
     2281 agctgctcgt aaaggcggtt acgctgtcgg cggattcaac acaaacaact tggaatggac
     2341 tcaagcaatc cttgaagcgg ctgaagcaaa acaagcacca gtacttatcc aaacatcaat
     2401 gggtgcagct aaatacatgg gtggttacaa aatctgtaaa gatttaatca ctgatttagt
     2461 tgaagcaatg aacatcactg ttccagttgc tatccactta gaccatggtg attacgaagc
     2521 tgctttggaa tgtatcgaat taggctatac ttcagtaatg ttcgatggtt cacatctacc
     2581 atttgaagaa aacttgaaat tagctaaaga cgttgttgaa aaagcacatg caaaaggtat
     2641 ctcagttgaa tgtgaagttg gttcaatcgg cggagaagaa gacggaatca tcggtaacgg
     2701 cgaattagct gatattgaag aatgcaaaca aatggttgct acaggtatcg actacttagc
     2761 ttgcggtatc ggtaacatcc atggtcaata tccagaaaac tggaaaggat tagcttttga
     2821 ccacttacaa gcaattgctg atgctgtagg tccagacatg cctttagtat tacatggcgg
     2881 atcaggtatt cctcaagatc aaatcgaaaa agctatctca atgggtatct caaaagtaaa
     2941 tgttaacact gaattccaat tatcatttgc tgcagctact cgtaaatata tcgaagctgg
     3001 taaagataaa gaaggtaaag gattcgaccc tcgtaaatta ttagctccag gtaaagcagc
     3061 aatcgttgaa gatgctaaat ctcatatcga ctggttcggt tcagctaaca aagcgtaagc
     3121 atccacataa cagatacgat atataggaat ggagaacgac ttttgaagtc gctctccatt
     3181 cttttttact taaaacggca gaccatgctc gtttcttttt tttatctatg ataaaataca
     3241 agcatatcca tgaaaaaaga ttaataaaca aggtggaaac agatgaaaaa aatcgtaatc
     3301 aaaggaaacc gacctttagc tggtgaagta acgatcagcg gtgcaaaaaa tagtgcagtc
     3361 gcattgattc cagctgcaat cttagctgat tccccagtga tcttggaagg tgtgccggac
     3421 atacaagatg ttcattcatt aattgaaatt ttagagatca tgggcgcaac tgtccatttt
     3481 tccaacaaca tactagaaat cgatcctcga gaagtcgtat cgattccgat gccgcacgga
     3541 aaaatcaata gtttgcgtgc ttcttattat tttatgggaa cactgcttgg taaatttggc
     3601 gaagcggtag taggcctgcc aggtggctgt tatttaggcc ctagaccgat tgacctacac
     3661 gtcaaaggat ttgaagctct aggtgctgaa gtgacaaatg aacatggtgc catgtattta
     3721 cggacagaaa ataaaggatt gcgtggcaat cggattttca tggacgtggt ttcagtcgga
     3781 gcaacgatca atgtgatgtt agctgctgta aaagcaaaag gacagacagt aattgaaaac
     3841 gctgcacgag aaccggaaat catagatgtg gcaactttac tgaataatat gggggcaaaa
     3901 gttcgaggag cgggtacaga tgtgatccgt atcgaaggag tcgaaacatt acatggctgt
     3961 cggcatttca tgattcctga ccgcatcgaa gcaggaacct atctagcatt agcagctgca
     4021 gtaggaaatg gtatcaaagt gaaaaatgtg atctttgaac atttggaaag ctttatagca
     4081 aaattgcagg aaattggtgt ccatatgaaa atcagcgaag atgaaatcga agtttacccc
     4141 tcgaaagaat taaaacccgc aaacatcatg acctatcctt atccaggatt tgccacagat
     4201 ctgcagcagc cacttactgc gttactattg atgactacag gcacttcaga aatcatcgat
     4261 acgatctatg cgaaacgtgt gaaccatgtg ccggaactag cacgaatggg cgctgatatt
     4321 accgtggaag gaaatatgat catcgtgaat ggtccaaata aacttcacgg cacagaagtc
     4381 gtggcttctg atcttcgggc aggtgcttgt ttagtgattg ctggactatt ggcagaaggg
     4441 acaacgacga tttacaatgt agattatatc ttacgcggtt atgatcatat tatcgagaaa
     4501 ctaacagcct taggggcatc aattgaaatg atagaggaag tgggagctga atgagcgatt
     4561 atttgacaat ggcagaacta gagaacaaaa cgttaaaaga agtttatagt tatgccaaag
     4621 aatttaaaat tccttattac agtaagatga ataaaaagga attatctcta gctgtgattc
     4681 gtgcacaagc agagaaacaa ggattttatt atatggaagg aatattggat atcgttggtc
     4741 aagatggtta cggtttttta cggccaatca attatggacc aagtgcagaa gatatctata
     4801 tttctgcctc acaaatcaga cgattcagtt tgagaaatgg ggacaaagtg gcaggaaaag
     4861 cccgtccacc taaagaatcg gaacgttatt atggtttgat gcacgtggaa agcgttaatg
     4921 gaaaagatcc ggaagaagca aaagaacgtc cgcattttcc agccttaaca cctctttatc
     4981 cgcaaagaca gcttcaactg gaaacaacac cgcaaagact atctacccga atgatcgata
     5041 tcttttctcc aatcggtttt ggtcagcgtg gattgatcgt cgcaccgcca aaagcaggga
     5101 aaacttctgt attaaaagaa atcgcaaatg gtatcaccga aaactatccg gaagtagaat
     5161 taatcgttct actgattgat gaacgaccgg aagaagtaac ggatctagaa cgaagcgtga
     5221 aaggcgacgt ggtatcttct acatttgatc tgcagccgca aaaccatact cgagtttctg
     5281 aattagtatt ggaacgcgca atgcgtttag tcgaagataa acgagatgtc gttatcttga
     5341 tggatagtat tactagactt gctcgggcct ataacttagt cgttccacca agcggacgta
     5401 cattgagtgg cgggattgac ccagccgcat tgtataaacc taagaaattt ttcggggcag
     5461 caagaaacat cgaagaaggt ggcagtttga cgattttagc gaccgcttta gtcgatacag
     5521 gaagccgtat ggatgacgtg atctatgaag aattcaaagg aactggaaat ctagaattac
     5581 aactttcacg tgaattatct gaacgtcgga tcttcccagc catcgatatc aaaaaatcag
     5641 gtacccgaaa agaagaatta ttgatgccaa gtgaagaatt agaagaaatt tggaaacttc
     5701 gaaaaaatat gactggagat tcattagaat atacggaaca actgatccgc tttttgcgta
     5761 aaacaaaaaa caatcagcaa tttttcaaag aatttcaaga tgtctccttt ggtaaaaaaa
     5821 atccgaccag aaatctgaaa agataattgc aaccttgact gaaacatgtt aagatgttaa
     5881 tgttgtaaat cgacaaatca actctggttc agcaaatgga gcagggcaaa ggagcgaaaa
     5941 aatcatgaaa gaaaacatcc atccagatta ccacccagta gtattcatgg attcaacaac
     6001 tggtttcaaa ttcttgtcag gttcaacaaa aacttcacaa gaaacagttg aatgggaaga
     6061 cggtaataca tacccagtca tccgtgtcga agtaacttct gactcacatc cattctacac
     6121 tggacgtcaa aaattcacac aagcagacgg acgtgtggac cgtttcaaca aaaaatacgg
     6181 tctcaaagac gcaaacgctg cggaataata aaaacgctga taactcaacg tttagcagac
     6241 tatctgatag ggatagtctg tttttttatg ttttttgcca atttaaaact tcgtttgatg
     6301 ctgaaaaagt cgtaggatca taatgcacaa aacaatgaaa aactagtata tagttttaat
     6361 tatccacttg ataaaaacac cttatattaa cgaaatgaag aggggttatt tcgaagtaaa
     6421 aaaccattta cctaaaatta gaagctaagc tttcctttca aggaaggctt tttgttttgt
     6481 tatagacttg cgattgtttg tgagctagat cgttcatttt aatgacttat acacgcggat
     6541 gatgggtctt tttcctctgt tcattcataa tattgaacgg tacagctagc gatgctatac
     6601 tgacctcagt tccaagaaaa cgttaacatt ttttgtgggg gagaggtttt ttatgaggtt
     6661 tgatcaattg atcgcagaag aactgattgt tttcgatcca tcgatagatt caaaaggatc
     6721 attatttgag cgaatagggg atgtgttgaa aaaaacaaat aaggtaagaa atgttcaaaa
     6781 agtcgttaag gcactattca aacgagagga ggagatctcc acaggaatag aagatggatt
     6841 tgggattccc catgcaaaaa gcaaatatgt gacaaaacca gcactgtgtt tttttcatac
     6901 agccaaatta acggactata gcgggataga tgaaatgcca attgaatata tttttgccat
     6961 cgtagttcct gaaaaatcag cagatatgca tctagagatt ctcagcacac tctccagacg
     7021 attgatgaat gcagatttta gaaagggaat aaaagaagcc actacgccag cagatatcat
     7081 cagacagatt agtcaagaat gaaaatagta gagtctttgt ccgagtatcg atagtgaaga
     7141 aaaaggagga aaataatcat gaaaatcgtt ggtgtaactg cttgcccgac tggaattgct
     7201 catacttata tgtcggcaga aaaattagaa gtaacagcaa gaaatctaaa ccatcaagca
     7261 aaattcgaaa cacaaggtgt aaaaacagaa aatcagttat ctgaacaaga aatcaaagag
     7321 gcagatgcaa tcattcttgc agtagataaa gaaatcgaat tggaccgatt tgccgggaaa
     7381 aaagtaaagc gtgtctctac aagccgagca atcaaagaac cgcaagtagt aatagacgaa
     7441 gcattgagag atattggcgt attcgttgtg tctaatgaaa aagaaccagc tgcaaatgag
     7501 aaaaagccga caatctataa tcatttcatg aatggtgtga attacatgct tccatttgtg
     7561 atcgcaggcg gaatcatcat cgcactgagt tttgcctttg ggattacagc ggctgatccc
     7621 aactcagcag actataatgt gttagcagct gcattttcaa ggatcggagg agatacagcg
     7681 tttgccatga tggttcctgc gttaggcgcg gggattgcta catcgattgc tggaaaagca
     7741 ggttttgccc caggaattgt tgccggatta ctggcctcta caggtggttc aggttttctt
     7801 ggcggaatga ttggtggcgt gctagcaggg tatatctgtg attttctggc aaataaaatt
     7861 agcgtaaaaa aagaattttc tgctatttat cagctgattg tcgtcccctt tttatcgatc
     7921 ttaattattg ggcttttgat ggtatttgtg attgaacaac cgatcacatg ggtgttggat
     7981 gctttgacaa attggctgaa tagcttggga aatacatccg ggctattgtt cggattactt
     8041 atcgggatca tgatggcagc tgatatgggt gggccgatca ataaatcgac ttcgactttc
     8101 tcgatcggtt tgatgtcagc tggagtaaat gcaccgattg ctgcttgtat ggctgcagga
     8161 atgacacctc ccctaggtat tgctttagcc actttattat tcaaacataa atttaccaaa
     8221 gaagaacgga cttcaggtca atcatgctgg attttaggcg cttcgtatat tacagaaggt
     8281 gctataccgt ttgcggtatc tgacccgatc cgcgtgatac ctagtttgat gacaggttca
     8341 gcagtcgctg cagggatttc catggcagta ggagttactt caatggcacc tcatggaggg
     8401 atttgggtga tgctaattcc aaatgttatg aacaatttac ctatgtatgt cttggcaatt
     8461 gcatcaggta cagtcactac agcctttatg cttggactat tgaaaaaaac agtgaaagag
     8521 aaaaaacaag acaagtggac gaaaaatgaa aatctaacaa atgaggtgga agcgtaatgt
     8581 tatatacaat gaaagagtta ttgaaagtgg ctaaagagaa tcaatttgca gtaccagcct
     8641 tcaatatttg cagctatgat atgatgaaag caatcatcga agaagcagag agattagaag
     8701 caccagtaat cttagaaatt catcctgatg aaatcgccta tttaagtgat gagtttgttg
     8761 cggcagtaag agcctatgct tatacaagta aactgccgat cgttatccac ttggatcatg
     8821 gcggtacgat agaagatgtc ttacgggcga ttcgaaacgg ctattcttcc gtgatgatcg
     8881 atgcgtcttt gaaatcattc gacgacaatg tacagattac gaaaaaagtg gttgcgttag
     8941 ctcatgcggt aaatgtctca gtggaggctg agcttggaac gattgggaat aatggttctg
     9001 ctgaaggagg tgctgaaaca atcatttata cagatcctga tcaagcgaag atttttgtga
     9061 aagaaacagg aatcgataca cttgctgtag caattggaac agcgcatggc ttgtatccga
     9121 aaaacagaac tccaaaatta aatatcgact tactgaaaga attgaaccaa caaatcgatc
     9181 ttccatttgt tttacatggc ggttcaggta atccggacgc agaagtcagc gaagcagtaa
     9241 aatacggagt tgccaaagtg aatttgagct cagatcttaa aagcgtcttt ttcaatgctt
     9301 tgagagaaat attgagtgaa aatccatcga tgtacgagcc gaatgcaatt tatccagcag
     9361 ctaataaaaa ggtacaagaa gtggtcaaac ataaaatgga aattttgaac acagtaggca
     9421 aggctagttt atactaaaaa taaaaatgtg tggtcaagag ggtatgacaa tagtcttgtc
     9481 ataccctctt aaccacacac gatgttcaga agtagttttt tcgaaaaatt tcttcttatt
     9541 tctcgaagcc aatcacttct gtcacaacct cttttttcct gttactatat aaagtaaatg
     9601 gggtgatttt atgttgaatg aagtggagaa tagacagcag gagattgtga attatctaaa
     9661 aaacaaccaa tttgcaagag tcaccgattt gatcgagtta gtaaattata gcgaagccac
     9721 gattaaacgt gaccttgttt ttctggaaaa aagtggattg atccgtagag ttcgcggtgg
     9781 cgccatgctt attgacaatc gaaaaattga cgtgccttac ctgatgaaga tcaccaaatt
     9841 agaagaagaa acagaaaaac gtaagattgc agatacagct tcttctttga ttcgagatga
     9901 tatggtctta ttcttggatt ccagtacaac ctctttacac ttagtcagaa acctagcaag
     9961 atttgatggt ttgcagatca ttacgaatgg catcgtcaca gctgctatgt tgagtgaata
    10021 tacaacggca aaagtatctg tattgggagg ggcaattgtt ccgaaacgag caacaatcaa
    10081 cggttcaaaa gcctataacg atgcattgtc atattatgcg gatttagcga taatgagttg
    10141 tcgcgggcta gatttcagcc aaggagtaac agaaactcat gaaggagagg caatggtcaa
    10201 aaaagctttt agaaaacaat cagaccggct tgccgtttta gttacacagc aaaaaattgc
    10261 acataaattc atgcatcaag ccattgcttt tcatgaaatc gattatctgg tgacaagtaa
    10321 agaactgaca aaagaacagt atgcgaaagt aaaggaacac catatcgaat gtctgtacgg
    10381 ataaattatt acgtgaatgt tctaagtggc ttcctcattt ttagatagga ccttatcttt
    10441 gctcgcttct ctcagccgat ggtataataa tgaaaaggtt atcttgttag ataatcttac
    10501 ttttttatcc aggagaaagg agtgcagaaa atgaaacatg tgaaggcgtt ggtagtgaag
    10561 gcaatcatga tctgggcgat tctgtgggtc gtgctgacag gtttatacgg tgttagcttt
    10621 atggattcaa cgattgttgg ggtaattatt gtagtcatga tctatgtgct aggggatctg
    10681 ttgatcttac gtaaagtagg gaacatcgca gcgacgatcg cagatgctgg ttcagctgca
    10741 gttatcttgt ggctctatct atattttatg aatgatacag tcgatatttg gatgaaagta
    10801 ttgattcctg ctctattgat cggtgtggca gaatggttct tccacaagtg gttgctaagt
    10861 caaggcatcg taccagatga acggaaaatg gaataaatca acagttaaac tattcttatc
    10921 gataaatgaa agctttaaaa ataaatagat gtttaattaa gaataatcat aaactatttt
    10981 tactcaaaaa accgctcaga ttattttcag atctgaacgg ttttttttat ctattcatca
    11041 agatcaaatt tcatttttca atgattcaat caattcgccc tctgcttcat gactatgttt
    11101 tcggccatta acaagataga gtgctgtaca tatcaatgga atgactccgg caatcacata
    11161 caatcctcta aatgaagtaa gagattttag catccccatc aaataaggac caacgcctaa
    11221 gcctaggtct aaaccaataa agtaggtaga taaagcaata ctgattcgat gttcgcggac
    11281 gatttttaaa caaacagcct gtccatttga cataaacgta ccataaccta ggccgactaa
    11341 tgctccagag ataagcaatg tcacactatt cgtcgttaca ctcagtaaaa ataatcctgc
    11401 tgttaagaat aaatagctag gatacatcac gtattgttct cctttgacat cgaaaatcct
    11461 tccagtcaat ggacgtgtaa atgtgatgac caaagcatat acgacgaaga aaaatgaccc
    11521 agcagcaact aagtggatta cttttgtata agaagataaa aatgataaaa cacttgaata
    11581 agaaagaccc atcaaaaagg caattcctgt aatgaaaagt gctttctttt caataaaact
    11641 attgatattc catgagcgaa gagatatttt ttgttcttca gataatggaa tattttttac
    11701 aggaaagaca aggcaggcaa tcgtaacaaa gaagaccagt acaattgaaa accagataat
    11761 gaaacgaaaa tcagtcatat tcagcaaaat cattcctaaa aatggcccaa cagcggcagc
    11821 taaactagta cttaatccat aataattgat gccttcccct tttttagatg cagggatata
    11881 tgcagtaacg atagcatttg tagctgtaga aactgtaccg tagccgaatc cattcaagaa
    11941 tcgaactaaa tacaatgccc cgatagttgg cgtataaaga tatgctaatg ttgaaaggag
    12001 atagaagatc gcgccgcctc ttaaggtaaa cttccgacca aatagttcta actgtttccc
    12061 catgaataag cgtgccagca atgttccgat gatatagatc ccagaagcaa aacctgcttg
    12121 gctcaagctg gcgtgtaagg tgtcttgcgc aatgactgcc atgatgacca tcaacagata
    12181 ataaatcagg tagacaataa aattgatcaa tgtgattcca ataaaatatt tattaaataa
    12241 tttcttttcc atgtatgtta tacattcccc ttaagctgaa tatgctttta atttttgtat
    12301 ttgaacttct acatcgtgat aattggatgg atagcgatca acaagttggc gaataaataa
    12361 atctcttgta gacataaaca gatttaaaaa ttcttcgcat tcgtttgttg gattctcatc
    12421 cactgctttg atcaattcga tcgagttttg atacgcatca tttaaaaaga ctaaagaagc
    12481 tttcatatca aatcttgttt gatatgctgt ttctaatcga ttaatgattg tgttcatcca
    12541 gtaattcatg ttttttccct ctattcttaa aaaattttac ttctgcagta taacgaataa
    12601 aaaaaaggaa aaaaatcgca taagtgatgg aaaaaggtac ttaacaaatg ttaatatcat
    12661 tagtaaaata cctttggact ttaaaaagtt ttaaagtact gtatgatctt tttagatgaa
    12721 agtttggatg ggggagcatc attggatagt agagtcttaa aggaatattt ggataaaaat
    12781 gattttccga ttatacgcaa aggatatcat aaatatttga cattcgaagg gatggaagat
    12841 tcccatacgt atatcttgaa agagggaacg gtaaaagcaa gtgttatttc aaatgatggg
    12901 agagaattta accttcgcta tatcaaagat cttgaaatcg tctctctttt aaaagacgaa
    12961 tattcacaat tcatcgattc gccgtataat attcgagttg aatcagaata tgcagagttt
    13021 tatcgaatca atcgtgtgcg cttttggcaa aatatcaacc aagacccaaa gctacaacac
    13081 tatgtaaaag agtattaccg ctatcgtctg ctttattcga tgaaaaaaat gcagcaaatg
    13141 ctattaaacg gaaagctggg agctgtttgt acgcaacttt acgaattata tgatttattc
    13201 ggtgtgcgga cagaagaagg gatgctgatc gatttttcga tcacgaatga agaaatcgcc
    13261 aaattttgtg gcatctcaac tgctagcagt gtaaacagaa ttttaaaaca actgaaaaaa
    13321 gatggtatta tcaccgttaa agaccatcat atcgttattt tgaatatgga cttgttagaa
    13381 gacaatatta tcttttgatg aacaaagagg ttgtgaaatc agcgttttga cctgagtatt
    13441 agagaaacaa acggaaaaat agttcctcat attttgccgt tttttgagct aataccgagg
    13501 gtcagctgat tgaacaccgt ttaccaagag gttgtgagcc tgccgtttaa ctctgagtca
    13561 caaggaacaa tttcaaaaaa cagcttttca tgtttgatga atattgtgac ttgtgaccga
    13621 ggagttggta ggagaacacc gtttaccaag aggttgtgaa aaagctgttc tgcatcaagt
    13681 aataagaaga gccaagtaaa aacagtctct tatgttttta acttggctgg acttatcacc
    13741 gtagatgtaa gcttttgaac gccgtttatt tgcaacaaaa ggggctgtga cacgattttt
    13801 gtcacagccc tttttttgcg aacattcatt tagcaatttt tagaaacttc tgccgccacc
    13861 gccaaaagtc ccgccaccgc tagaatgggt agtacttccg ccaccgccgc ttccaggagg
    13921 tgggcttttg gggattcttc ttgtcgtgac aaaagaattt gtcagacgat cggttttttc
    13981 agttaatttg atactggatt tttctcggaa tggatatttg tagatgcctg atttcaattg
    14041 ataacgcgaa acagtcacaa aataaaagac aagactaagg ataagagcca aaacaacagc
    14101 aatgatgatt tctactgtcg tcaaaacctt atagcgtgta attttgccag tttcttcgtc
    14161 tacacgataa tgtcctcctg gaactccgtc ttccacatat gttgctgatt tagacaggta
    14221 agcttttgca gcattgtaat aggcagcatt tgtcatctga tcataaacat catctaagat
    14281 cgaatcgatt ctgctatcag tcaaataatc gatcatatta ccagaagtcg aaatgtagat
    14341 ttcacgctgt cccatatcta atagcaatac tgagccattt tgatcatttc cgactgcgtc
    14401 tcttaagtaa ttatcagcaa agtctcttgg ttcttctgaa ttagatgtag tcgttacgat
    14461 caagactcgt cctttgattt tttcattcag cttttctgcc tgttgttcca aagattggat
    14521 ttgatctgtt gtgaataacc cagcctcatc atttaccgta gctgtatctg catgagtagc
    14581 caccggccag ccaacactta ctagcaaaag aaagaataaa aaaaactttt tcatcatatc
    14641 aagtaacctc cgatcagaaa gaggatcgct agcccagcga aaatcccaag agcggtcagt
    14701 cccaattttt tctggctgat cggcaggaca ccgctgacct ttcctgtttg tccattcatt
    14761 gcgtagtagt agactttttt acttgagtca ttgcttcgat aagtaacaag ccataccggc
    14821 agtaatacgt aattatttac ttcgtttgtg attgaagcag aagttcgcac accggtcaat
    14881 gttgtatatc catttgcggt atcttttaac agcttctctg aatagtcttt taattcagat
    14941 tgaatctgtg atttgattgc ctcatattcg atatctcttt tttctgcttg gaacccggct
    15001 aaatactggc ttttaaaatc aattgcttta tctaacggga aaggttgcac gccttcgacc
    15061 atcttttgtt gcgtattttt tgataaggca ttttttacta gttctttaaa agaaagcttg
    15121 cctgctcttg aaacagcaaa ttgcttcgtt tctgtgtatt cgatgtcacc tatccgccat
    15181 atgcggataa ctgtaccatt cgcctgcatg gcaccatcta cttcagcatc tgtagcccaa
    15241 taagggaaat acacacctgt caacttatcg atttgatcct tattgaaaaa agctttggga
    15301 acaaaccatt ttttcttggt ccaagcgaga aatctgttga ttgcttcttc tttctcgaca
    15361 gcaaaaggaa gtactttatt tggcaagaat tctccgctta aacgtcccga tagtacgacg
    15421 ggattatggc agtagtaaca ataagtcgca gcagttgtcg catcagtcac gatctctgca
    15481 ccacagttgg gacaaagaaa aagatctatc gctgcggtat tatctgcaga ttcgtccgct
    15541 gtatctgaaa aaccgcctgc ctcgtcaacc gctttgcgct cggcttcatt catatcggca
    15601 agctgttctt gggctgtaaa agtaaaatct tcggtaggtg aaggttcttc tttgctttct
    15661 tcgccagcca tacgcgcttc tttttgcttt tgctcatatt ggctgacttc ttcttctgta
    15721 tagatattca ggcagtattc gcaatgaaat ttttggtctt tgggatcaaa agtcaacgga
    15781 ccgccgcaat taggacattt atgtgtaaga acatccatca gctgcacacc tttctaaata
    15841 aattaattaa cggggattcc tttaaacggt ttaccacagt ttggacagaa tttagggatg
    15901 ccgttggaaa gatccacgat ctcattgcat tcactgcatc gcatttgtag cttaggctga
    15961 acgttttcta caggtttggc agctccgcag ttggaacaga attttccgga attttctgtc
    16021 ccgcaaactg gacatgtcca agtatccgca tttccttgtg cagtttgttg attttgtttt
    16081 tctgcttgtt gtttgatttg ttcttggtta ttttgagctg cttgagaaag ataagaacca
    16141 tttgcattca ttcccatacc tacacccata aagccggtca ttgctcctgc ttcatttttt
    16201 ccggctgctt ccataccacg tgcaatggaa ccttgcacgt atccttcacg tacgctagga
    16261 tcacctagca tcgccccttc gttacgcata ttgatcagtt tcacagaatc atcggtgtaa
    16321 gaaatgctgg ctacagccac agaaacaatc tccatcccac gtaattcacg ccaagaatca
    16381 tccaatgcag tatccatgta tttgcttaat tccaaactct tagatggtac ataagaaatc
    16441 cgttgaccat ctgcagacat ctgattgatt gctgattgta gcgctgttag gaattcagct
    16501 aaatactgct cattgatatc attgatttcc acttgtgttt tgtttttagg gattgcgttt
    16561 gtataaaata aaatcgggtc aacgacatga atagaataag tgccatgtgc ccgtaagaat
    16621 aattctgcgt tatagaaatt gtcaaagtag ttcaacggcg cgcttgtacc gaatttgatt
    16681 ccctttattt cttggagatt gatataaaaa acttgttgtt tctgcggtgt gacgccgcca
    16741 aatttgaatc ggtcaaaagt ttcagcaatt gcttctttca gtgttccatt gaacatggaa
    16801 ggagctgcgt catttttaat ggtgtaataa ccttcctcag cggtataatc gatgattttt
    16861 ccaccatcga ccaaaagcat catcatgttt ggatagacat ggacgactgt tccgtctgtt
    16921 aaaacatctt ctgttccttt acgattggag ccacgtttgt catctttgcg gactttgaca
    16981 ccttttgtca tgaccgttgt atcactcata ttgtctggtt cgatgacttc tagccattga
    17041 tctgccagac cgccgccaac catacttgtt gctgctttga taagacccat aaaaaattcc
    17101 tcctctgatt cagctagtgc caaacaatta ttgtttattt attataccat aggaagatgt
    17161 gaaacttctc ggactagaga cggaattgaa atggaaaatt gcgtattgtt taaatacctg
    17221 aaaaagccga gaaggatagt agttttttta cctaatatca cgtataataa gagatagtga
    17281 attttttaaa aaagggggat ccttatggca tatcaagcac tttatcgagt ctggcgttcg
    17341 cagcgtttcg aagacatcgt tggacaaaaa gctgtgacac agacactaaa aaatgctatt
    17401 gtttcccaca aaacctcaca tgcctacttg tttacaggac ccagaggaac cggaaagacg
    17461 agtgcagcaa agattttcgc aaaagcaatc aactgtccga acagtaaaga cggtgagcca
    17521 tgcaacgaat gcgaaatgtg tcgatcaatt actgctggta cgcaagaaga tgtgatcgaa
    17581 atcgatgctg ccagcaataa cggagtcgaa gaaatccgat ttatccgtga tcgggcaaat
    17641 tatgcgccta caaaggcagc gtacaaagtg tacattattg atgaggtaca tatgctttca
    17701 acaggagctt tcaatgcctt gttgaaaaca ttggaagagc caaaagaaag tgtcatattc
    17761 atcttagcaa cgaccgaacc tcacaaaata ccagctacga tcatttcacg gactcagcga
    17821 tttgatttta aacggatcaa tacggcagat attgtcgaac atcttgaatt tatcttgaaa
    17881 gaatcgacta tcagctatga accagctgca ttgaatgtga ttgcccgttc tgcagaaggc
    17941 gggatgcggg atgcattgag tattcttgat caggcaatct cgtttagtga aggaacaatt
    18001 actttagatg atgccatgca agtgacaggt agcttaactg acgaaatgat ggacagttac
    18061 ttgagtgctt gcgtgaaaaa agaagtacca gaagcattgg aaacattgaa ccaaattctc
    18121 gcagccggca aagaatcacg tcgctttcta gaggatatgt tacaatactg ccgtgacttg
    18181 ctgatgtatc agcaagcacc taacctagtt gaggaacaat cgagtacgat cagaagtaca
    18241 tttacagaat tggcacaaac tgtttcagcg gatcagctgt tttcaatgat aaagattttg
    18301 agcgatactc aaaatgaaat tcgttttacc aatagtgccg ctatttattt ggaagtcgct
    18361 acagtcaaac ttgcacagct accgtctgct tctgttcctt cagcagatgt agcatttgca
    18421 gatgatcaga tagtaaaaca gctacaacaa gagattaagg aactaagaaa cgaaatcaag
    18481 gaactacgca gtaatacagg aattgagaat acaaaagtac agccggtaca gcaaacaaat
    18541 cataaaaaac agacaagtac ctatcgtgta ccgacggaac gagtatataa agttctgcgg
    18601 gaagcgacaa gacagcatct gaaccaagta aaagaagtat gggaagactt gctgatgagt
    18661 ctatctgtca ctcagcgagc aatgcttaaa gcaagcgaac cagttgcagc tggacctaca
    18721 ggactagtga ttgcgtttga ttatgagatt gtttgtcaaa gagcagcaaa tgacgaagaa
    18781 ttgcagcttt cggtacacaa caacttaagc cgaatgatca aagattatgc gcctgatcct
    18841 gtttatatta caagagaaag ctggccagaa cttcgaagaa attatttgac gcaggcacaa
    18901 gaagagggaa cagaaaactc ttttgcttct gaagactcag aaggagaaat cgagttgctt
    18961 cctcaagaac aagaagtacc agaagaaaca cttgtagtaa aagcagagga actgtttggc
    19021 tccgaagctg ttacagtggt agatgattaa taaaaaggag tagataatta tgatgcgtgg
    19081 aatgggcaat atgcaaggca tgatgaaaca agtacaaaaa atgcaaaaag aaatggcaga
    19141 agcacaagag ctgctaaata aaacagagtt tacaggtaaa gccaccaatg aattggtcaa
    19201 agcggttttt acaggcgatc gtcgcatgaa agatatccaa atcaatgaag aagtcgttga
    19261 tcctgatgat atcgagatgc ttcaggatct tgtcgtaatg gcagtcaatg atgcgatcgc
    19321 acaaattgat gtggaaactg agaaaaaatt agggaaatac gcaaaaggtt tgccaggatt
    19381 ttagttttaa actgaaggag taatgaatat gcaatatcca gaaccaattg cgaaactgat
    19441 cgacagctac atgaaacttc caggaatcgg tcagaaaaca gcgacaagac tcgcctttta
    19501 tacgatcgat atgaaagatg aagtagtaaa tgaatttgct aaatctttgt taagtgtaaa
    19561 acgcgatttg catttttgta gtatctgcgg aaacatcaca gaagaagatc cctgcgaaat
    19621 atgtagaaat ccttcacgtg atcgaagcgt tatcctagtg gtcgaagagc cgaaagatgt
    19681 aatggcaatg gaaaaaatgc gagaatatca cggactttat catgttctcc atggtgtgtt
    19741 atcaccgatg gaagggacag gtccagaaga catcaatatt tcttctctgt tgcagcggct
    19801 gcatgatgca gatgtaaaag aagtgattat cgcaacaaat gcaacaacag agggagaagc
    19861 aacagctatg tatctctcac gtctgatcaa acccgcaggt atcaaagtga ctcgtctagc
    19921 acatggtctg tctgtaggct ctgatatcga gtatgcagat gaagttactt tattgaaagc
    19981 tgtcgaagga agacgtgagt tataaatttc taattaagtg ttttttcaga tacaatataa
    20041 aagcagcaca agagatgggg gcaacaatgt gaacggacta tttattacaa tcgaggggcc
    20101 tgatggtgca ggaaaaacca gcatcttgaa tgaactttac cctcttttga aagaagtagc
    20161 aaaaacagaa atcattcaaa caagagaacc aggaggcatt ccgattgctg aagagatccg
    20221 agcagtgatc cttgatccaa aaaatgatcg gatggatgaa cgtacggaag cactgcttta
    20281 tgcagcagca agaagacaac atctagtcga aaaggttctg cctgctctgg caaaagggaa
    20341 aatcgtttta tgcgatagat ttgtcgacag ttctcttgct tatcaaggtg caggccgacg
    20401 catcggcgtt accgagattg ctcggttgaa tgagtttgct acagaaggta ctacgccgga
    20461 ttttaccctt tatcttgacg tagattcaga caccggatta agaagaatca agaaaaacag
    20521 acaaaatcaa atcgaccgtt tagattcgga aggtttggaa ttccaccagc gtgtacgtca
    20581 tgcttactta aagctggcag aagagaatcc ggaaagaatc cacaaagttg atgcaagaaa
    20641 aagctttgaa gaagtgctgc aaacaagtta tcatacaatt attgaacagt atccgcaatt
    20701 ttttgaaaac taggaaggtg aacgtttatg aaaattattt tagcgattat ccaagacaaa
    20761 gacagcaacc gtttatcaaa tgaattgatt gatgcaaata tccgtgccac aaaactatca
    20821 tcgacaggcg gcttccttaa ggctggaaac agtacgttta tcgtgggtat tgaagatgac
    20881 cgtgtagaag aagcattgga aatcatcaaa aaaacgtgtg aatcaagaaa gcaatttgta
    20941 tctacacctg ttacactgga tatttcgatg gatgggggcg tcccttatcc agtagaagta
    21001 gaagtaggcg gtgccacagt cttcgtctta cctgttgaag gattccatca attttaggag
    21061 gagctgagct atgaatcaag aaataaactt ggctcaaatg caaccgatcg tgtatcagca
    21121 gcttcaacgg agttttgagc atgggcgtat cgctcatgct tatctttttg aaggggagaa
    21181 aggaacagga aaacatgaag taggtatatg gcttgcacag cacctgttct gtacccaaat
    21241 gaaagacaat ctgccttgtg gaaaatgcaa caattgtcag cggatcgaaa agaaagaaca
    21301 tccagatgtt ttggttattg aaccggaagg acaaaccatc aaagtcgatc agatcagacg
    21361 tttgcagacg gaattcagtc gcagcggtta cgaatcaaga aaaaaagttt ttttgatcaa
    21421 agaagctgaa aaaatgaata gcagtgcggc aaacagtttg ttgaaattct tggaagagcc
    21481 ccctggagat tttttagcgg tgttagaaac agatgcagta ggaagaattc tccctactat
    21541 tcaatctcgt tgtcaagttc tgcattttca agaactttcg aaagaagcac tgatccatcg
    21601 attgcaagaa gagcagattc cattggaaaa agcaaaactt ttggctttct taacaaacag
    21661 tttaggaaaa gcagttgaaa tctctcaaga tgaatggttt aatgatgcta aggacttgat
    21721 ttatcaatgg ttcgtttact tgcaaaagca agatactcaa gcctttatct atgtccaaaa
    21781 aaaattggta aaatcattta aagaaaaaac acaacagttt acaggattat ctgtcttgat
    21841 gttttattat caggaagcac agcgtcaagc agtagcaaac ggcgaatatg gcaaagtgaa
    21901 acagataaac cagataattg aacggatatt gatcgcagag cagaaactac gcagcaatgt
    21961 cagttttcag gctgttgcgg aacagttcgt tttgcatacg attttttagc ttgccgcaaa
    22021 ggatgatgat caaggaggat ggaaatggta gaagtagtag gtgtccgata tagagatgca
    22081 ggtcatatct attattttgc accaggagac tctgaatatg tttatagtga caaggtatta
    22141 gtcgaatctc agcaatcctt acatattgct actgttgtcc ttcctaaaaa agagattgat
    22201 cccaatgacc ttcctgaagc tatccgtccg atcttgcata aagcgtcaca agctgaatta
    22261 gaaaaagaca aaaaaaacaa aaaagatgct tatgatgcat tgaaaatcgc aaaagaaaaa
    22321 gtgcagttgc gtgagttgga aatgaaaatg gtcaaagccg aatacacatt tgatcgaaat
    22381 aaattgattt tttctttcac agcggacggg cgaattgact ttagagaatt ggtaaaagat
    22441 ctggccgctg tatttcgtac tcgtattgag cttcgacaaa ttggtgtccg cgatgaagca
    22501 aagataatag gcggaattgg tccatgtggg cgtccattat gctgttcttc ttttttaggc
    22561 gatttcgtgc ctgtatcaat caaaatggca aaagatcaaa gtttatcttt gaatccaaca
    22621 aaaatttccg gtttatgcgg acgcttgatg tgctgtctgc aatatgagag cgaagaatac
    22681 gagacagcga aaaaagaact ccctgacttt ggaaaagtaa ttgctacacc tgatggtaaa
    22741 gggaaagtcg tagggttgaa tctgcttagc cgtatcgtta gtattcggct gactggacgg
    22801 gaaaatcctg tcgaatatga atgggatgaa ttgaaagaat ttatccaagt aggtgagatg
    22861 aatggataag agatcattat acgacggatt gaatcaatta gaatcagagt tgagaaatac
    22921 attaacagaa cttgttgaaa tgaagcatgc tttgcatgag atcgtagaaa aaaatacaac
    22981 tttggagatg gaaaatcaac gtctgcggga acacctgcgg gaattgaacc aagttgcaca
    23041 agctccagct gaaaatgtga agcaggaact ttctaagtct cgtatgaatc ttgaaaaaat
    23101 ttatgaagaa ggattccatg tctgctacga tttatatgga tcaagaaggg acaatgacga
    23161 gccttgtgcg ttttgtctag aagtgatcta tcgcgaaagc ggtcgatgac caataacaag
    23221 aggagactgg agcggagaaa tactgctcca gtctttttat taaggagatt gtgacagatg
    23281 tataatcaaa aaagtttcaa gggaaatccg gtatatgggg atttatatct tgtaccgacg
    23341 ccaattggaa atttagaaga tatgagctac agaagtgttc gtatgctgca agaatccgat
    23401 cttatcgcaa gtgaggatac gcgtaataca caaaagttat tgaatcattt tgaaatccat
    23461 acaccccaaa aaagtttaca tgaacataat tataaagaac gtgtccctca attgattgct
    23521 ttgttaaaag aaggaaaaac gattgctcaa gtcagtgacg caggaatgcc ttccatcagc
    23581 gatcctggac atgagctagt tttagcctgc ataaaagaag ggatttctgt gatctctatc
    23641 ccaggaccta cagctgggat gacggctttg attgcttcag ggctagtgcc tcaaccattt
    23701 ttattttatg gcttccttcc acgtaagaaa aaagaacaga aagaaacatt gaaaaagcta
    23761 aaagaacaga cagttacgct gattttttat gagtcacctt atcggatcac tgctacattg
    23821 acgaattttt tagaaatttt tggaaatcgc caagtcgttt tgtgtcggga attgacaaag
    23881 gtccatgaag aataccttag aggaagtacg gaagagctac tcgcttatat cgaagaacat
    23941 ccagtaaagg gtgaatgttg tctgcttgta gaagggaatt tgttcgaaat gccagtgacg
    24001 gaagttgatg aagagttggg aacgttaaag gaacaagtag aagcaaagat cgcttcaggc
    24061 gaaaaaccaa atgctgctat caaagcagta gcgctgaaaa atgggctgaa aaaacaagaa
    24121 gtctaccgtc agtaccatga attagactga gatagtataa gttgtctata atgaaagact
    24181 aatgaggaaa tcagctacta acttttcaaa tctcttgaac gcgaacgaat gttcttgtat
    24241 aatgaataag aggtgaggat gatggagctg caatttcctt tacataatga tacatcaaga
    24301 aaaatcattc atattgatat ggatgctttt ttcgcttcgg ttgaagaacg agatcatcct
    24361 gagtttcgcg gacatccatt agtaattgcc agacacccca gtgacactgg tgggcgtgga
    24421 gtagtgacta cagcaaatta tgaagcaaga aaattcggaa ttcattcagc aatgagtgca
    24481 caaaaagcgt atgagctttg tccgcaagcc atctttaagc ctggcgacca tcataaatac
    24541 agcgatattt ccaaacaagt acgggaagta ttctatcgtt atacagatat tgtggaacca
    24601 gttagtatcg atgaagcgta tcttgatgtg accgaaaata aaatcaattg caagtcagcc
    24661 atcaaaatcg cccgaatgat ccaagcagat atttgggaga gtgtccagct aacatgttct
    24721 gcgggagtaa gttacaataa gtttttggcc aaattagcat cagactatca aaaacctaga
    24781 ggaatcacgg tagttgcccc tcaagacgct gtgccatttt taaaagcttt gccaattgaa
    24841 aaatttcatg gggtagggaa gaaaactgta cctagaatgc atgagctagg gatttataca
    24901 ggaaaagacc tatatgaatg cacagaaatg atgttgattc gaaattttgg aaaaatgggc
    24961 tattctcttt atcgtaaagt cagaggaatc catgattctc cagtaaacgt tacaagagag
    25021 cgaaaatctg tgggaaaaga gcatacttat gggcagccgt tgcaaacgga agaagcagtg
    25081 ctgactcagt tgcgccaact agctgaaaaa gtagaagaag cactgagaag agtccaaaaa
    25141 catgggaaaa cagtagtttt gaaggtcagg tacacggact attctactgt gaccaaaaga
    25201 gtgacattgc cagaatatat ctataaaaaa gaagcactct tttatcaagc aagccttata
    25261 tgggaggaaa tcttgggagt agaaaaagga atccgattgt tagggattac tttgacgaat
    25321 ctggacccga tgacttatga aaacatcgtc cttccattat gggaaaatca agaaatataa
    25381 aaacagcttt gagagttgaa accggaaatc cgttttctct cccaaagctg tttttatatc
    25441 aagtcttgct ttttgatttg ttcataggtt tctgttgcat ccgtacattt ttcccaaatc
    25501 acaacttgat tggattctaa agatttttgg acgtcaatga tccgctggtt gctacttccg
    25561 cgaaattgta gttttaggtc acgtttgctt aattcaaagc gtccgtcaac aaggatatcg
    25621 atttgcgaaa ggagttctaa tttatcttct gaatcttgta gcaattcatc aaaggcatag
    25681 cctgtccatg accagatatc ttttgtcgta ccgaactctt ttcggatacg tcgtacgact
    25741 tgcagacaaa catctgtatt caaaaaaggt tcgccaccaa gtaaagttaa tccttgcacg
    25801 taatcgttgc gcaagtcagc gatgatttgt tcttctaatt caggtgtgaa tggtttgcca
    25861 tatcggaaat tttgtgcagc tttgttgaag cagccttcgc aggcaaataa gcagccacta
    25921 acgtaaagac tgttgcggac accttcacca tcgacaaaat taaacggttt gtagtcagca
    25981 atatagtttt ggctgtagtc ttttgctaac cattcttttg gttttggatt tctcataaaa
    26041 aaacttcttt catttaaggt tcaaagatat agctattgaa gataaaatct actcgaccac
    26101 ataaataaga acaaactgac ctaaatccat tgttcaagaa agcttgttct tattttgata
    26161 gagggcgttc aaagatacaa agccatcgaa agagggtggt gaaaaaagac ttgtcatagc
    26221 ctcttattct gaataaacgg tgttcaaaag cttgcatctg cagtaataag cccaactaaa
    26281 cgaaaaatat gaggaactat ttttgtttag ctattcttat tacttgatgc aggacagctt
    26341 tttcacaacc tcatttcatg tgtttcacac gtgaggaaat ttctttatgg cggccatgta
    26401 ccatcggacg agcttgcgga tttcctaaat agccacaagt acgtttgact acatcacatg
    26461 tttttggatc atgatttcca cattctggac attcgaaccc tcgttctgtc ggtttgaaat
    26521 caccttcgaa accacattga tagcaatgat cgattggcgt attcgtacca agatacccga
    26581 ttttgtcata tgcaaaatcc catacagctt ctaaagcttt cggattttgt tggagcatag
    26641 ggtattcaca gtagtggatg aatccacctg aacaatattt tggataatcc ttctcaaatt
    26701 ccaatttttc aaatggcgtt gggtttttac gcacatcata gtggaaactg tttgtataat
    26761 attctttatc tgtgatattt tcgatgatgc cgaatttttc tgtatccaaa cggcagaaac
    26821 ggtcagttag actttcactt ggagttgaat agacactgaa atgataacca tattcatccc
    26881 cccatgcatc tgcattagct tttaattctt tgacgatatc aagtgtgaaa gcttttgctt
    26941 ctgggtttgt ttcccattcg ccaccgaaga aagctgaagc aacttcatac aatccaatgt
    27001 agcctaacga tacagtagct cgacggtttt tgaataattc attgacagaa tcggtacgtg
    27061 aaagacgttt cccgaaagaa ccatacatat ataaaattgg cgcatttgct ggaatggctt
    27121 ctttacaacg ttctacacga tagagaagcg catctttcat gatttctaag cggtcagaca
    27181 gtaactgcca gaatttattc tgatctcctt ttgattcgat cgcgattctt ggcaaattca
    27241 atgtaacaac acctaaattc attcgtccga catttacttc ttgaccattt tcgtctttcc
    27301 acccttgcaa gaatgaacga cagcccatag gtactttgaa acttccagtt aattcaacga
    27361 ttttatcgta attcagaata tcaggataca ttcttttcgt tgcacattcc aatgctagtt
    27421 gtttgatgtc gtaattagga tcttcagggt ttaagttcac accttttttc aaagtgaaga
    27481 tcagtttagg gaagatagct gtccgctttt cactccctaa tccattgatt cgaatctgta
    27541 aaattgcttt ttgaatttct ctttcaaacc agttttctcc tagtccaaat cctaatgaag
    27601 tgaaaggagt ttgtccattt gaagtaaata aggtattgat ttcatattcc aaactttgca
    27661 tagcatcgaa gatatctttt ttggtttttg cttttgcata ttctttttgg cgttctggcg
    27721 aatcgatcca ttcttccgcg tcttttagat gttttttata gttcaattct gcaaaaggag
    27781 caagcaattc atctgttcgg tcggcagaac agccaccata ttgacttgac gctacattgg
    27841 caataatctg tgagatttgt gctgtggcgg tctggataga ttttggcgat tcgacttcgg
    27901 cattgccgat tttgaaccca ttgttcagca tgcctttgaa atcaatcaag cagcaattcg
    27961 tcatcggtgt ataaggatga taatccaaat catggtaatg gatatcgcct ttttgatgcg
    28021 catttgctat atgtggtggc aacattttca gtccgattga ttttccaacg atcccggctg
    28081 tcagatctcg ctgtgtattg aaaacattac tgtctttatt tgcgttttcg ttcacgacag
    28141 tttggtcctt gttgattagc ttttcaatcg aaaagttgat atctgttgct ttactgcgag
    28201 caaaatctcg tttggtacga taattgatgt actcttctgc taattcatat tcgttttttt
    28261 ctagtaatat atgttccacg atgttttgaa tctcatagat tttgacattt tcagcaaacc
    28321 ggcttttgat ttcttggaca accaagtctg caatcatttg aattttttca taagtaagtg
    28381 gtgataatga gccgtgaatt ttcttttctg ctttgatcag tgcttcattg atcttttgtt
    28441 catcgaaatc aacatggcgg ccatcgcgtt tgatgacttt catctgggat gtctctggct
    28501 gctgagtaac gacagttttt tcttcataat ttactttcat tatcttcaac tcctgctaaa
    28561 ttgttcttgt ctatcataaa acaaaatcgg ggaaggagca actaaatatc ggctatatat
    28621 agtattcaat gtgctgtata attggatttc tatactatat gtagtgtata ttttattgtg
    28681 aaaaaaggtg aaaacttgta gctggtggat ttgttttttg catatttaat tttgctgaat
    28741 aaaataattg aataaaattt tttattcagt ccaagtaaaa gtgacaaaaa cataagagca
    28801 aattattcgt agatatgcta ctatgcaaat aaatagaaat gaaaaatgaa agaggaggag
    28861 aagaattgga aaagatgatc gaagtgaaac atctaagtaa aagctacggc agcaaacttt
    28921 ctcccgtcaa agccttagat aatatctcat tcacagtaga agaaggcgag tttgttggga
    28981 ttatgggccc aagtggtgca ggaaagacaa cgctgatgaa tatcttgtcc acgatcaatt
    29041 tgcctacgat gggcagtgtc tcgatccaag gaaaagaaat cacaaaaatg aaaaacagtg
    29101 agctcagtga ctttcgtcga aaaaaactag gattcatttt tcaagagttc aatttgatcg
    29161 ataccctaaa tgcaaaagac aatatcctat tgcccctagc agttgaacgg atgactaaag
    29221 aggaaatgga aaagagagta agacatgtag ctcaattgtt gaatatagaa gaactgctca
    29281 aacggtatcc cgatgaactt tctgtcggac agagacaacg aattgcagct gctagagcac
    29341 ttgttgttca gccacaagtg atttttgcag atgaaccaac tggctctttg gactcgaagt
    29401 cggctacaga attgttgaac tacttgaaga ccatgaatca aaaagaaaaa gcaacgatac
    29461 tgttagtcac ccatgatcca tatacagcaa gctactgcga tagaatttta tttattaaag
    29521 atggtgtgat tttttctgaa gttgtcagaa gagggacaag aagagagttc tttgagaaag
    29581 taatagacat gcaggcgacg attggcggag gaggaaaaat gaatgctgta taaactagcc
    29641 gttaaaagtt tttttcggca gtcacgaggc tatcttgttt attttttcag tttgacttta
    29701 tccgtaatga tctattattc cttcagcgca atgacctacg atcaatcttt agtcagaaga
    29761 gcaagtcaag atacttcgat agatggtgga ttgagttttg gtggatttat cattactgtt
    29821 gttttgcttt ttttcgtatt cagtgccaac cgatttttct tgaatagaag gcaaaaggaa
    29881 ataggcatct atcagctttt tggtatgaat aaactgcaga tatcaggtat atatgtgtta
    29941 gaaatcatga taattggatt gttcgcctgt atttctggga tattactcgg tattattttt
    30001 tccaaactct tttcaatgat tttggttcga atgatggata tggatcttac tagtccattt
    30061 tttatctcca ttccatcagt agtagatact ttatttgctt ttttcatcat ccttttagca
    30121 gtatctgtat attcaatatg gaaaatatgg cgctatccaa tgattcgaag ttttggagaa
    30181 aaagaacaag cagatagttc aacaatgcgc ttcaggacaa ggcaccgatt actcgcagtc
    30241 atcgggttac tacttttaac gagcggatat tttggagcta gtcattttag ggaaatcact
    30301 tcatggatga tcagttatca ttatcttgat acattagttt tcttttttcc ttttttaatt
    30361 tttttcgttt gtgtggtcgg tacttatttg tttttctgtt atacatttcg gttatttttg
    30421 atcatattga gtaaatggaa gatttattac tatggtatca attttttgat aatgggtaac
    30481 acgcaagttc atttgctcaa aagttggcgt acaaattctc tgattacagt cattctcgga
    30541 atttctttaa tgacaattgg cggcacggct agtatctcta ctattttatc ctaccaggag
    30601 aaagtttcct ccccgatgga ctatcagttt gatgttgaga cagagaaaaa aatcaagccg
    30661 attctagcag aggaagggca gaagataact aaagaaatca ccttgcatta taaagtcatt
    30721 gggagtatct atagtcaaaa tctcgaagaa cctatgtttc aaactgctaa tctgatcacg
    30781 gaaacacaat atcaagcctt cagaaaagtt aatccagatt tgccgaacat ttctttgaaa
    30841 tcacctggtc atactgtttt gcttgatcaa atgcaaactc tttttcggaa tatttcatta
    30901 tatggatcta gtattggact gccagataat caagaattga agattcaaca aatgcttcca
    30961 agtgtattag gtgacgaaac aatggtatat ctaagtcctg tactgattgt agatgatacc
    31021 gtttttgaaa aagcaaaagg tgtggattat caagtagtca atgtagatgt atcaggtgga
    31081 aataatgaaa aaatagatga acgactgagc aatgacctgt cgataaagtg gctcaatgct
    31141 gtttattatt cttatgatat cgtaaatgga agtctacaag gagaaattag cacaaagaaa
    31201 cttccggatg accaaatgaa ggaagcacaa tttagccaag aaagctctcg tttgaatttc
    31261 tcttccagct attcgaaatt gcgtgcagtt aatcggcgaa tcggtatcaa tatttttgtc
    31321 acactatttg tcgggatgat cgtgattatt gctactggta gtattttgac agtccggcaa
    31381 ttagcagaag cagaggaaga aaaagaaaat tatcacctat tgactaagct ggggattcca
    31441 caaagaagga tcagacggat gatcttcgag caaaacgcac ttaccttctt tccacccatg
    31501 atattaggga tcactcacgc agtatttgca atcaatgtct tcactcagta tataaaaact
    31561 gccgattact ggttggctta tcttgtgagt ggcttattga tccttatcta tcttttgctt
    31621 tatgtcatta cgagtcagtt atattgtcgg attatcgagg aatgatatac ggaataagac
    31681 aagataataa atagaactaa attttgaata ccatttattt ggaataagag gttgtgacac
    31741 gatttttgtc acaacctctt tattttttat attttttaaa agtacgagaa attataaaga
    31801 aaaaagcagt gatttgatgt gctattttta taaaagctat gtgacaaaac tgttagtaaa
    31861 aaaacggttt ctgtaagcta gacacatgtt tctttttgtc aaaattagtt acactttgtg
    31921 tgaggttata tgaggaggga atccaatgaa caaatggcgt tctaatccaa aattgaatta
    31981 ttggggaaaa ttcatcattc gaacaatttt ttatagtgca gtactgctgt tcctgattta
    32041 tctgtatcac tataaaaata ttcaaggagg aacttttatc tataacgaat tttaggagga
    32101 agagtatgga aatcaaaacg attattgaag caatcgatga ttggggaata aatgaacctg
    32161 atcgagttgc ttatcaggcg gatgagacgc atacttttgg tgaattgaaa caagcatcag
    32221 acgcacttgc ttattattta gaaaaaaagg tagaaggcga tggaccaatt gtcgtttttg
    32281 gcaatttgga gtttgaaatg attgtctcat ttctaggggt tgtcaaatct ggccatgcct
    32341 atttgccgat tgaagagcat acacccaagg agcgtatcct atcgatcttt cgggtggcta
    32401 aaccatctat ggtcatcagt atcggggact ggatagaaga tattccttcg gttcctgtca
    32461 taacaaaaaa agaatttcaa gaaatcaaga agataccagt aggattttat cccagaaata
    32521 gcgtaaaagg tacagaaaat ttttatatta tttttacttc tggtactact ggagaaccaa
    32581 aaggcgtaca aatcagtcat gataatctag tcagctttgt tcaatggacg atggaagact
    32641 ttgggatcaa gcctggtatg catttcttag cccaagcgcc attttctttt gatttatcgg
    32701 tattttctat ttatcctgca ttagtttctg gcggaatgct gaagccgtta aataaagcag
    32761 ttgttcaaga ctttcgtcaa ttattcgcga cattgccagc cttgaagttg aatgtgtggg
    32821 tttctactcc atcatttatg gatatttgct tgatggaacc tacattcaat ggagaaaatg
    32881 tgccggaatt ggacatgttt ttattttgcg gagaagaatt gacgaaaaaa actgcagaaa
    32941 gtctacttga tcgattccca aatgcacgga tatataatac ttacggtcca acagaagcaa
    33001 ctgtagctat ttcaagtatt cagattgacc agcatgtact agatagttat gaccgactac
    33061 cgattggcta cgtaaaaaaa gacacgaaag tttctattgt ccaagaaggt aaacctgttt
    33121 caaaaggaga aacgggtgaa atcattattg caggaccaag cgtatcaaaa ggttacctga
    33181 acaatcccga aaagacagct gctgcgttct tttgtcaaaa tgacttagca agttaccata
    33241 caggggatgc aggtagacta gatgaatatg ggcttttatt ttatgaagga cgtatggatc
    33301 agcaggtcaa attacatgga taccggattg aactcgggga tatcgaacat tatctgctcc
    33361 aagacaaccg aatcaaacaa gctgtcgtcg taccaaaata ccaaggaaca aaagtacagc
    33421 agctggttgc ttttgtcgta ttagaaaaca aaacgaaaca gccggatttt cagttgacta
    33481 aatctatcaa agaacaatta ctagaagttg taatggatta tatgattcct cagaagttcc
    33541 attatgtcga tctgctgcct cagacagtta atggaaaaat cgatcgaaaa aaattaacag
    33601 ctgaggtgaa tcctgaatga tgttacctca tatgatccct tatgcagagc cgttttattt
    33661 ttttctttta gccattgcgc ttgtaccgat cgttctatct ttattgatat gggaaaaaag
    33721 attaccagtt tatcaatcag cagtgacact tttcttttta tttgtcagct ttggaggtaa
    33781 ttattggcaa cagggagcgg cgctgattgg gtatgttttg tggcagacga tacttgtatg
    33841 gggctatttt gtttatcggc aaaggaaaaa tcagacagga tggttttttg ccgccgttat
    33901 tttagcgata attccactgt ttttaacgaa agtgactcct ttttttgaac atggaaaagc
    33961 atcgttactt ggttttttag gaatctctta tttaaccttt aaatctgtac aaatgatcat
    34021 ggaaataaga gatggcatga tcaaaacata taagccattt cgttatatcc agtttctgct
    34081 gttctttcca acaatctctt ctggaccaat cgatcgctat aggagatttg aaaaagatca
    34141 gctccatccg cctgttcgtg ataaatatgt tgatcttttg ggaaaaggcg tccaaaatat
    34201 ttttgtcggt tttttatata aattcattat cggttattat tttggtcaag tactgcttcc
    34261 tgtagtggga cgcattgcct tacagcatgg tggtatttca tgggcattag taggttatat
    34321 gtacgtgtac agtatgtatt tgttttttga ttttgctggc tatagcttat ttgcagtcgg
    34381 tacaagctac atgatgggat atcagacacc aatcaatttc aataaacctt ttttatcatg
    34441 gaatatcaaa gaattctgga atcgttggca tatgacgctg tccttttggt tccgtgatta
    34501 tgtatatatg cgattgatgt tcttcatgat gaagaaaaaa ttttttaaaa gcaagatcgt
    34561 aatgtcaaat atcggttatt ttgctttgtt tcttctaatg ggtatatggc atggattgac
    34621 gtggtactac atcgtatatg gactgtatca tgcttgtctg atctgtctga cagatgcatg
    34681 gatccgattt aagaagaagc ataaagagca gttgccttct aacaaattta cgcatggctt
    34741 agccgtattt ttgacatttc aagcagtatg ttttagcttc ctgatttttt caggattttt
    34801 agataaactt ttttttagct aacgaatagt gacatattaa tttatataac aaaggagaaa
    34861 aggaaaatgg aagaacaaat tttatcaatc ttagaagaaa taacaggaac agatgaagta
    34921 agaaaaaatc gtgatattga tctatttgat gaagggttgc ttgattcttt agcatctgtt
    34981 caattattag tcgagctaga cggagagctg gatatccaag tccctgtatc tgaattcgag
    35041 cgagaggatt ggagcacacc gaataaaatc attcaacaag caactgcatt gaagggttga
    35101 gtatgaaaaa gaaacttttt gctattttcg gcccggttgt tttagctgtt ggcttactgt
    35161 ttgtgttttt tacctcctcc tatcaagtag atatctctaa tccagaaacg attagacgtg
    35221 cctcttcttc catgtcagtg aacgtgttga aaggagatgc gataaaaaat tatgcattat
    35281 ctgaaaaaca atatattcct ttttttggct caagtgaatt aagcagaatc agtccatttc
    35341 atccgtctgt tctggcagaa aaataccagc gaaactatcg tccatttttg ctgggagcac
    35401 ccgggacaca atcgttaagc caatatatga tgatgcgttc tgcaggagat gctatgaaaa
    35461 acaagaaagt cgtctttatt atttctcctc agtggttcgt taaaaatggt gtaaaaactg
    35521 attactttaa tacgtattat tctgaacttc aaacatacga ctggttgttt tcaatgaaga
    35581 aagtaacgcc tgctgatcgc tatttagcta gaagattatt aactttctca aaagtaaaag
    35641 agaatgatac gttgacggcg attctgcaaa cgatcaaaaa agggaagcta cctttacctg
    35701 aatctttgaa tcagctgcgc agccaatgga acatgctgaa gagagaggat gaagtattca
    35761 gcaatattgg gttgaaagat cgacagcaaa aaatcgatca tgagtcaaaa cgattaccaa
    35821 aacaatacca agaaacagaa ttatccattt tagcaaatca aattggagaa cgagaaacga
    35881 cgaataatcc attcgggttg aaaaacgatt tttatacgca tcgtatccga gcccacgagc
    35941 cagagctcaa acagtcacaa aaaaattggg attatcgttt ttcgccagaa ttttctgatt
    36001 ttcagctggt cttggatcaa ttagcaaaaa accataacga agtattgttc atcatcccac
    36061 cggttaacga gaaatggagc gattataccg gactatctca agaaatgtta caaggatttg
    36121 ccaaaaagat caaatttcaa ttaaatagcc aaggattcaa tcgaatagct gattttgtca
    36181 atcaagcggg taccaactat tttatggaag acacgatcca tctaggctgg aaaggctggt
    36241 tggctgcaga tcaacagatc agaccgtttt tagaagaaaa tcatattact gctagtaagt
    36301 atcatcttga cgatgctttt tttagcaaat cttggcaaca tcaaatacca gataaactac
    36361 aattaaaata aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt ttgaatattc
    36421 tgaataaaag ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa tggtataatc
    36481 acaatatgaa tatagaaatg ggggaaaaca tttggataca aaagaagaac agctacttat
    36541 tgatttaact tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag aagtattcag
    36601 agaatatgcc aaaccattcg ctgatgatat tttttatgac ggtttaggca gtgtgattgc
    36661 caaacatggt gctggcggag gccccaaggt ctttatttct ggacatatgg acgaagttgg
    36721 ttttatggta acaaaaataa ccgaaaaagg attcttggaa ttccaaactc ttggaggttg
    36781 gtgggggcaa gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag ggaaaatcgt
    36841 ccacggtgtg attggttcca aaccaccaca tgttttaaca cctcaagtaa gaaataaacc
    36901 ttatgaaata aaggatatgt tcattgatat tggcgcatca agccaagaag aagccaaaga
    36961 gtggggcata cgtcctggag atatggtcac tccatatatt gaatacaaac gaatgaatgg
    37021 cagtaaatat ttactggcaa aagcatggga taatcgtatc gggacagctg tttctctgcg
    37081 agtattagag aatctatcta aagaagcaca cccaaatgtt ttattcgcag gaagcgatgt
    37141 gcaagaagaa gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa atcccgatat
    37201 tgcttttgct ttagatactg gtacagcagg agacactcca ggaatgacac caaaagaagc
    37261 agattcgatt ttaggaaaag gcccacagat tttgattttt gatgcatcta tgatcccgca
    37321 taaaaaatta ttgaattttg ttattgatgt agcagaggaa atggatattc cattccaata
    37381 tacagtgatt tctggcggag gaactgatgc aggacaaatg catttgacaa gagatggtgt
    37441 tccgtctgta gccattaccg ttcctgttcg ttatctgcat tcccatacat cagtgattca
    37501 tgaagatgat tatttgaata cagtgaaact agtcacagaa ttagtgaaac ggcttgatag
    37561 tgaaacagtt gaaaaaattc ggagttatga ataatgaata atagaatacc tatttctgaa
    37621 tatcggaaaa aagcacgagt ttctttggaa aatcaatggg gaatcaatac ttggcttgtt
    37681 ttcttatcta taatatttac gagcgtcatc cagaactttt ttgacggttt cagtcctttt
    37741 ccggaagaaa gcatgcagca gcacttattg agttttctag tcgataattt acttttattc
    37801 gcattcaact atgcattgtt ttacattgcg ctagtagttg tacggggtgg cagaccaaaa
    37861 agtaaccttt tattttcagt tttccaaaga aagtattttg gttcgatact tggcatcaat
    37921 ttgatcaatt cagtcatcaa ctggctactc aacagtttaa tctttctgcc catgttttta
    37981 atcagcggag tgagtactta tatgcagctg cttttcagtt caaatgcggt acctacgggt
    38041 gtcatgccag aagattttct tgatatcagc tttgttttat cgatgatagg gatgattttt
    38101 gtttcagcac tcatcatgag tattgttggc ggtttattcc aatttgctgc atggacaaag
    38161 ttcgactatc ctgatttgtc ggttattcaa tgtttgaaat atggctggtt tttattgaaa
    38221 gatagaattg gtacgtatat tttgttgcag ctgtctttta tcggctggta tttgctagga
    38281 gcaatcgctt tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt gtcagttgca
    38341 gaattttatg aacaagcacg gattgaaaaa ggaggtccag acgcctattt tacttagcat
    38401 gtaagcagtt tattcatcaa aaaaataact tttgtggtag gataaatctt tgagggggaa
    38461 tgtacgatga aattaactgt tttaggatgc ttaggagcgt atccttataa aaatcaaggg
    38521 acaacaggtt atttgcttca atctgacggg ttcaatttgt tgatcgatgc aggtagtgct
    38581 acgctgatca aattacaaga acaccttgat ccacttgatc tagatgctgt gatccttagc
    38641 cattatcatc atgatcatat tgctgacctt ggtgtattac agtattactg gcaacttcat
    38701 cctaagagaa atccggagac attgctttca atttatggac acacaaaaga ttccattcac
    38761 tttgaagaat tgacgttgga aaatgtctca aaaggacatc cttattttga agctgaagaa
    38821 ttaaaaattg gtccttttct gattactttt atggaaacaa tccatccagt agtatgttac
    38881 gccatgcgta tcgtagaaga aacaacgggg aaagtattcg tatttacagg tgattcaggt
    38941 tatttggaaa gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc agatacctat
    39001 ctttttgaag gaaatgaaca tcatcatgcc catttcactt caaaagaagc tggagaactt
    39061 gcgaaatcag ctaacgtaaa gaaacttgta cttacacacc tacctcagtt tggtgattta
    39121 gaacaattga aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat agaacttgct
    39181 gcagttgaca aagtgtttaa catttaggag gaagaacatg atttttgtac caaatgaaaa
    39241 ccacgaccca agaatcaatt tagcaattga acaatatttg ctgcaagaaa tgcctttaga
    39301 tgaacctatt ttgctctttt atatcaatga accttcaatc atcatcggca gaaaccaaaa
    39361 tacgcttgaa gaaatcaatc gagattatgt ggaagaaaat ggcattcatg ttgtccgtcg
    39421 cttaagtgga ggcggagctg tctaccatga ttttggaaac ctgaacttta gttttatcat
    39481 gcctgatgat ggggattcat tccgtgactt tgcgaaagta acacagccaa tcatccaagc
    39541 acttcatgaa ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc tagtcattga
    39601 tggcatgaaa ttttctggca acgcgatgta tgctaccaac ggtcggatgt ttgctcatgg
    39661 aacaattatg ttcgactctg atatcaatga agtagtcaat gcattaaaag taaaaaaaga
    39721 caaaatcgaa tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata tcaaaccatt
    39781 tcttccagaa gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa tcttgttgaa
    39841 aattttcggc gtagagtcag tagaagaagt aaaaacgtac gaattgactg atgaagattg
    39901 gaaaaaaatc aatcgaatct ctgaggaata ttatcgaaat tgggattgga attacgggaa
    39961 gtctccagat ttcaatttct ctcgccaaaa acgtttttct atcggttcga ttgaagtcca
    40021 tttgaatgtc tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt tcttcggact
    40081 aggagatatc tctgacgtgg aaaaaaaatt ggttggacag aaatatgata aagcatctct
    40141 atcaaaaata gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag aaccagaaga
    40201 attgcttacc ttactttatt gaaaataaaa aatagcggaa tccacatata tggattccgc
    40261 tattttttat acaggattcg ggtaatttcg agagagttga acagtgatcc gatcaatgaa
    40321 agtatctttt tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt tcatcatcat
    40381 taagtcaaaa cgttcctgat catctaattc ttctgtgatg cctcgactaa ataggtttgt
    40441 atcgtaatgt gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt cgtgtatcga
    40501 caaataaatt tttcgataca gtggattttc gatggcatga gtttctctaa aggaatgctc
    40561 taaagcgtgg atcaaaagtt gagccagatc gtcatcgttg ataggaagtt cttcgggaag
    40621 atctgcttgg aaggttatct gaatattccg tttggcagct aacttctggt aacgcgctaa
    40681 gataagattt gtcacctggt ggttgcaaaa agaacacagc atttcgaaat tttttgcttc
    40741 ttctaatatc ttttccacat agtttttagc ttttgtttgg tccgtatgca ttaaatcttg
    40801 cagattctcc atctcttgga caaattccag gcgaagtgta tttacttctt ggatatgctg
    40861 tcgaagctgc tcataatgtt gctccaacaa atcatttttg atttttaaga aatctacttg
    40921 tctccgatct ttgtcggtat ttaagatgtg atcgattaga ttgtagctga taataacgat
    40981 tagaatgata atatataaaa tcgtagacca attgatcgtt gtttgataga tacctaatgc
    41041 cgtttggatg taaatcaagc aatggataat ggctaccaag acgatccaag gtgtacaagc
    41101 tacataaaaa cggttattct tatatgcttc gccaatgcat gcaagtgaag tgactaaagt
    41161 gcttataaca ctagcaatat tagcaatcgg cattaattca ggaagttctg caggagtaaa
    41221 agttgcataa agcagaacgc cgataaaaat caaaagttgg aacgtacaaa aaattccgta
    41281 ccatttttta tagtaaaaca ttcggctcca atagtagcag atgatgaaag ctgacgttaa
    41341 gataataaat aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa taccacttaa
    41401 aatatcttct gatacagctt caaatcctaa tgccaaagca aagcaactta agagaataag
    41461 tgagtattca agacgttttt gtttatacat caagtagatc gtatagaaaa aggtccctat
    41521 agcgataaaa acagcgatta tcatcgtcaa tacttgcggc actgattgtg aataaataaa
    41581 actaatcaat ggtccagttg ttcctagata aactttcggt ggcagtccag catagttctg
    41641 ataaggagat gagactgtaa gggtcaattt ttttccgata taatttgctg gtaaatctat
    41701 cacagccaat gagagtccgg gattgctttc ttcctgataa tctttccgtt ggtataaaat
    41761 cttttcgcct agatatacag taatccattg atggtttcct ttgattagta aatttgcttc
    41821 gtccaacggt ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat ccacggtagg
    41881 aatcgtacga gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg tatagaaaac
    41941 ccacgaatct tttatttctt tcatttgatt gctgtccgca ggagaaaaaa taaaataaaa
    42001 gaaataagca attcctgtta aaaataacaa tgataatata atgattatgc tccactttat
    42061 acgtaaataa aaagattctt ttttattat
//
LOCUS       DACDTO010000016        39140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDTO010000016 DACDTO010000000
VERSION     DACDTO010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.016"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(219..704)
                     /locus_tag="IWK70_000902"
     CDS             complement(219..704)
                     /locus_tag="IWK70_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9613468.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1184..1825
                     /locus_tag="IWK70_000903"
     CDS             1184..1825
                     /locus_tag="IWK70_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9613469.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1844..2479
                     /locus_tag="IWK70_000904"
     CDS             1844..2479
                     /locus_tag="IWK70_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9613470.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2497..3312
                     /locus_tag="IWK70_000905"
     CDS             2497..3312
                     /locus_tag="IWK70_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9613471.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3631..5067
                     /locus_tag="IWK70_000906"
     CDS             3631..5067
                     /locus_tag="IWK70_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9613472.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5256..7703
                     /locus_tag="IWK70_000907"
     CDS             5256..7703
                     /locus_tag="IWK70_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9613473.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7933..9000
                     /gene="rlmN"
                     /locus_tag="IWK70_000908"
     CDS             7933..9000
                     /gene="rlmN"
                     /locus_tag="IWK70_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9613474.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9193..10296)
                     /locus_tag="IWK70_000909"
     CDS             complement(9193..10296)
                     /locus_tag="IWK70_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9613475.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10849..11397
                     /gene="aac(6')"
                     /locus_tag="IWK70_000910"
     CDS             10849..11397
                     /gene="aac(6')"
                     /locus_tag="IWK70_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9613476.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            11943..13604
                     /locus_tag="IWK70_000911"
     CDS             11943..13604
                     /locus_tag="IWK70_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9613477.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(13674..14021)
                     /locus_tag="IWK70_000912"
     CDS             complement(13674..14021)
                     /locus_tag="IWK70_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9613478.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            14105..15046
                     /locus_tag="IWK70_000913"
     CDS             14105..15046
                     /locus_tag="IWK70_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613479.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            15047..16090
                     /locus_tag="IWK70_000914"
     CDS             15047..16090
                     /locus_tag="IWK70_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613480.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            16096..17166
                     /locus_tag="IWK70_000915"
     CDS             16096..17166
                     /locus_tag="IWK70_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613481.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            17156..18109
                     /locus_tag="IWK70_000916"
     CDS             17156..18109
                     /locus_tag="IWK70_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9613482.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            18121..19131
                     /locus_tag="IWK70_000917"
     CDS             18121..19131
                     /locus_tag="IWK70_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9613483.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(19416..20237)
                     /locus_tag="IWK70_000918"
     CDS             complement(19416..20237)
                     /locus_tag="IWK70_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9613484.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(20334..20612)
                     /locus_tag="IWK70_000919"
     CDS             complement(20334..20612)
                     /locus_tag="IWK70_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613485.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            20880..22889
                     /gene="metG"
                     /locus_tag="IWK70_000920"
     CDS             20880..22889
                     /gene="metG"
                     /locus_tag="IWK70_000920"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9613486.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(23368..23955)
                     /locus_tag="IWK70_000921"
     CDS             complement(23368..23955)
                     /locus_tag="IWK70_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613487.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            24130..25452
                     /locus_tag="IWK70_000922"
     CDS             24130..25452
                     /locus_tag="IWK70_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9613488.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            25607..26383
                     /locus_tag="IWK70_000923"
     CDS             25607..26383
                     /locus_tag="IWK70_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9613489.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            26376..26945
                     /gene="rnmV"
                     /locus_tag="IWK70_000924"
     CDS             26376..26945
                     /gene="rnmV"
                     /locus_tag="IWK70_000924"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9613490.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            26938..27822
                     /gene="rsmA"
                     /locus_tag="IWK70_000925"
     CDS             26938..27822
                     /gene="rsmA"
                     /locus_tag="IWK70_000925"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9613491.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            28273..28902
                     /locus_tag="IWK70_000926"
     CDS             28273..28902
                     /locus_tag="IWK70_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613492.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(29026..30135)
                     /gene="ugpC"
                     /locus_tag="IWK70_000927"
     CDS             complement(29026..30135)
                     /gene="ugpC"
                     /locus_tag="IWK70_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9613493.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            30344..30766
                     /gene="mgsA"
                     /locus_tag="IWK70_000928"
     CDS             30344..30766
                     /gene="mgsA"
                     /locus_tag="IWK70_000928"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9613494.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            31089..31625
                     /locus_tag="IWK70_000929"
     CDS             31089..31625
                     /locus_tag="IWK70_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9613495.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(31665..33428)
                     /locus_tag="IWK70_000930"
     CDS             complement(31665..33428)
                     /locus_tag="IWK70_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613496.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(33431..35146)
                     /locus_tag="IWK70_000931"
     CDS             complement(33431..35146)
                     /locus_tag="IWK70_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613497.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(35925..36149)
                     /locus_tag="IWK70_000932"
     CDS             complement(35925..36149)
                     /locus_tag="IWK70_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613498.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            36349..39117
                     /locus_tag="IWK70_000933"
     CDS             36349..39117
                     /locus_tag="IWK70_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9613499.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTENVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
BASE COUNT        12825 a         6930 c         8034 g        11351 t
ORIGIN      
        1 cgatttataa tcgaaaaaag aagcattttt atatatgctt cttttttcgt agaatcaata
       61 aatatggatt tggctaacta tggaagaata ctcgtattag ttttcagcgt tcatactagt
      121 tgttacattg tttgattggc caaaagaatt tctgatatat tttgtgctaa ataaagaaaa
      181 agacgcttga attgggaaat aagaaaacta ctctatcatt acaaattttc ataaactggc
      241 agatgttcta agtaaacaag tatcttttca aagtcggaaa aatccataat caacgtagtg
      301 gtattgatat ttggatgaaa gcctacggta tcttgacgat caatagcagc atcgatcacg
      361 acttggattt ttttctcttt gtcgtgcatc aatgctaatg gagtcactgt acctgctgga
      421 acgcctagga ggtcaggaag ctgttcttca gaaacaaaag ataagcgctt ttcttttaat
      481 tgttcagcta gttgttttaa atcggcgtgc ttttcatcag gcaagatgac aagatagatt
      541 tgttttcctt ttcttgattt caataataga ttttttactt gcggaccagg tagggaaaaa
      601 gggacatttt tgactgacgt aatcgcagga tgatcgactt tttgaaaaga gatacccagt
      661 ttttttagta gttcataagc ttcttgttcg gttgcacgac tcatcgcgca tcaccctcct
      721 ttttttatta gtgtatcata aaaaagagtt gacaaatgaa aaataatcat gtaaattaca
      781 cgtaaagtta aaacaaatga catcgaaaag aagagtagcc gttaggtgtt tttagagagt
      841 ctctggttgg tggaaagagg cagcagttaa ccgtgaaaca catcttggag tcgattttct
      901 gaagcgtata gtaggaaaat ccggggagcc cgttacagct gcggtcgttg ctagatgacc
      961 agataaggct ggcttttccg tgaggagtcg gtaaaaaaag gtggaaccac gtgattaacg
     1021 tcctttgatc ttcggatcaa aggacttttt gttttcatct gaacggcaac gactgtgtga
     1081 ggttttttct gcaaggaaaa aataaacaga ggtggaacca cgtataatac gtcctcttgg
     1141 catttgccaa gaggattttt ttattttaaa ggaggaaaag aagatggagt atttgattga
     1201 agttatgccg caattattag taggagcact cacaacattg aaattatttt tatttacctt
     1261 gattggctca ttaccattag ggattgtttt agcattagga ttgtctttgc attttaagcc
     1321 gctacgctac ttgcttcaaa catatgtatg ggttatgcgg ggaacaccgc tgttgttgca
     1381 actgattttt attttttatg ggttgccgac gattgggatc gtttttgatc gtttcgaagc
     1441 agcactcatt gcctttatct taaactatgc agcttatttt gcagagatct ttcgtggagg
     1501 gatacaatcg attcctcaag gtcaatatga agcagcgcaa gttttgcaat taagtccctt
     1561 tcaaacggtg acgaaaatcg tcatcccaca agtgacaaag attgttttac cttctgtagg
     1621 gaatgaagtt atcaacttgg tcaaagattc ttccttgatc tatatattag gtttaggtga
     1681 cgtaatgcgt gctgggaaaa tcgcgatgga acgcgacgcg actttactgc ctttgctagg
     1741 cgtggcaatc atctatctta cattgactgg tattctaaca atcataatga aacaagttga
     1801 aggaaaatta aattattacc gctaactgat aggaggaaga aatatgattg aattgaagaa
     1861 agtgaacaaa gcatttggtg aaaaaaaaat aatcaaagat ttgaatctaa tcattccaga
     1921 tgggaaaaca ttagctatcg taggtccttc cggtggcggt aaaaccacgc ttttacgtat
     1981 cttggctggc ttagaaagtt ctgacagcgg agaattttat ttagacggtg taccgttcaa
     2041 cccattcgag cttaaaatga aagaacaagt tgtaggagtc gtattccaag acttccagct
     2101 atttccacat ctgaatattt ttgaaaacat taccttagca ccaaaaatgg tattgaaaca
     2161 gacaaaagaa gaatacacca aaaaagcaga agaattagcg gcttcattag ggattagtga
     2221 tttattgaat atctatcctt atcagctttc tggcggacaa aaacagcggg tagcgattgc
     2281 cagagcattg gctatgaatc cacgggtatt ggcttatgat gaaccaacaa gtgctttaga
     2341 tcctgaatta cgccagcaag tcgagcgttt gatcctatca ttgaaagaag atggggtaac
     2401 ccaaattatt gtcacccatg atttagtatt tgcagaaaat gtcggtgatc aattactaaa
     2461 agtttcccca attcattaag aaaggagaag atagagatga aaaaaaccag ttttctattc
     2521 gctttgatcg caggtcttct tctttttgca ggctgttcga acaaaaaaac ttcagcagat
     2581 aattgggcga ctatcaacga agagaaaaaa atcgttatag gtttagacga tacatttgtt
     2641 cctatgggat ttagagataa agacgggaag ctcacaggat ttgatattga tttagccaaa
     2701 gcagtctttg aacaatatgg gattacggta gactttcagc ccattgactg gtcaatgaag
     2761 gaatttgaac tgaacaatgg aacgatcgat ttgatatgga acggctattc caaaacgccg
     2821 gcaagagaaa agaaagtgca atttacaaaa ccttatatgg aaaacgatca agtgctgatc
     2881 acacctaaag aatcaggaat cacaaacttt ggacaaatga ataataagag attaggtgcg
     2941 caaaatgggt catctggtta tgatgtattt acgaaacaac cagaagtatt aaaagagata
     3001 gtcaaaaacc aagatgctgt tttgtatgat agttttaatg aagcattgat cgatctgaaa
     3061 agcagacggg tcgatggtct gctgatggat aaagtatacg cagactatta tttgaaacaa
     3121 agaaacgaac tagaagactt caacataaca aaaggttctt atgagagtga ggattttgca
     3181 gttggtgcac gcaagtcaga tagtacatta gtgaaaaaaa tcaatgaagc cctagatcaa
     3241 ctagagaaaa caggtgaatt ccaaaaaata tccgataaat ggtttggcga agatgttacg
     3301 ccgagaaatt aacaaatagg ggactgtgac aaaagacgtg tcacagtccc tttttccgaa
     3361 taaacagtgt tctcctgcca actccttgtg actcggagct gaatggcagg ctcacaacct
     3421 cttggtaaac ggtgttcaaa agccagtttt tcggtattaa gccaaattta tgcaaaaatg
     3481 tagaaagcca ttttcacaaa atttttctta ctactcgaaa ctaagcgact ttttcacaac
     3541 ctcctttaca taattttatt ttgttgtgga aggggtttcg tgttattcta aaagaggaac
     3601 gattgaaggt tataggaaag gaagagatga atggaacaca aagaacttac ctcatttcca
     3661 aaagattttc tgtggggctc tgcttctgca gcgtatcaag tagaaggagc ttggcaagaa
     3721 gatgggaaag gagaatcagt ctgggatcga tttgtacgga tacctggaaa gacatttaaa
     3781 ggaacgaacg gtgatttggc agtagaccac tatcatcgtt ataaagaaga tattgcttta
     3841 atgaaagaac aaggtctgaa agcttaccgc ttctctgtag cttggacacg gatttttcct
     3901 aatggacgag gagaagtcaa tcaagctggg ctcgcttttt atgagagatt gatcgacgaa
     3961 ttgattgaaa acgatattga acctatgttg actttatatc attgggatct tccacaagca
     4021 ttgcaagatg aatataatgg ttgggaatca cgtcagatca ttgaagattt cactcattat
     4081 gcagaaacat tatttgaggc attccgcggg aaagtccatt actggattag tttgaatgaa
     4141 caaaatatct tcacttcatt aggttatttg ttagcagcac atccgccagg tgtgacagac
     4201 ccgaaacgaa tgtatgaagt taaccatatt gctaatttag caaatgcatc tgtcatcaat
     4261 aaatttcacg agttagaaat gcctggaaaa atcggaccaa gctttgcgta tacgccaaat
     4321 tatccaattg acagtaatcc tgaaaatgtt ctggctgctg aaaatgcaga agacctgatg
     4381 gccaattatt ggatggatgt ctatatgtgg ggaaaatatc cgattgctgc catgaggttc
     4441 ttagaagaaa aaggatgggc accaacgatt gaagctggcg atgcagaatt gctagaatct
     4501 gctaaaccag attttcttgg gatcaattac tatcaaacgg cgacgaatgc attcaatcca
     4561 ttaaacggag ttggtgctgg aaaaatgaat acgacaggaa aaaaaggttc atcagaagaa
     4621 actggagttc ctggaatgta taaaaaagtt gagaatccat ttgtcgaacg aacaaattgg
     4681 gactgggaga ttgacccaga aggattaagg atcggactgc gtcgtatcac aagccgttac
     4741 cgcatgccgg tactgattac ggaaaatggc ttaggagagt atgacaagtt gacagaagat
     4801 aaacaaatcc atgacgacta tcgaatcaat tacttacaaa gccacgtcaa agccatcaaa
     4861 gaagcaattt cggatggagc agaagtccta ggctattgca cctggtctta tacagatttg
     4921 cttagctggc tgaacggtta tcaaaagcga tatggatttg tatatgttga tcaagacgaa
     4981 acacaagaag gatctttgga acgatataag aaagacagtt attactggta ccaaaaactg
     5041 attgaagaaa atggagagaa cctttagttc acatacttaa aacaagaagg tacggctgga
     5101 tatggccgtg ccttttcttg ataaaatcat ttagaaatta agaaaaactg taaaataagc
     5161 tgaaaaatga aagaaagcgt cagttctttc aaacaaaact ttagttccct tacttttttt
     5221 tgtataatga aacttgatga aatgagggat aataaatggc acaaaagaaa cggcctacta
     5281 aaaaaaggaa aaagacgaaa aaaacgaaaa agcaaaaaca gcagcaggaa caaatgctga
     5341 cgattggtgt tgggtttgcc ttgattttat ttgctgtctt cggcttttta aaattaggtt
     5401 ttttaggaat attgattgca aacggctttc ggatcattgc aggaaatact taccaaatcc
     5461 tctgtctttt attagccgta ttaggatttt ggattgtcat taagaataca gagttttcaa
     5521 ttggtaaaaa ccgacgctgg tttggcggca tactctttta tttcggtatc ttattattgt
     5581 tacatgccca tttatttggc aaactccata ctggggaacc aaatatcatg gggacgacat
     5641 gggatttgtt agccagtgat ataaaacaaa gtcaagtaga caataacgtt ggtggtggga
     5701 tgattggagc aatcctttat cattttactt acttcttgat tgcacagcct ggaagttatt
     5761 tagcagcagt gctattactg gcaggaggag cattcctgtt cagtaatctg gaaggctatc
     5821 agctgttaaa tggtatccaa tcaattggag aacgagtaca ggaattattg gaaggtgatc
     5881 ctgccaagca agctcgaaag caagcggcaa aggaagagag aatgaaacag cgcgcagaag
     5941 cgaaagaagc aaggcgctta gctgcaaaag aagctgcaga aaaagaagct gcagaatatg
     6001 agaagaaaaa agcggtgagc cagcaaaggg aaaatactgc tgatgaatgg caggaaccag
     6061 aaaatacaga gccggagcag ctaagttttg ttccaatcga cagttttcaa gaaaatatcc
     6121 atcctgcgaa tctagagaaa ccagtaccag atacaccaaa acagaccaat acagcagaag
     6181 gtttttcaga tgagttgcca gaggatgacg gtacctcatt agaatttgag attgaagcag
     6241 aacaggaaaa tcaagactat gaattaccga cagttgattt gcttgattct ataccaactg
     6301 tggatcaaag tgatgagtat aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt
     6361 tccaaagttt cggtgtcgat gccaaggtcg tcaaagccag cttaggacca tccgtcacaa
     6421 aattcgaagt ccagccggct gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg
     6481 atatcgcgct tgctttagcg gcgaaagatg tccggatgga agcaccgatt ccaggtaaat
     6541 cactgattgg tattgaagta ccaaatggaa agatcagtat ggtttccttt agagaaatca
     6601 tcgaagcaca accaaaccac ccagataaat tactagaagt acctttagga cgtgatgttt
     6661 ctggacgggt acaaacggct gatctttcga agatgcctca cttactcgtg gctggttcga
     6721 ctggtagtgg gaagtcagta gcaattaatg ggattatcac aagtatatta atgcgtgcta
     6781 agccccatga agtaaaactg atgatgatcg atccgaaaat ggttgaattg aatatgtaca
     6841 acggcatacc tcatctgttg acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct
     6901 tacaaaaagt agtacaagaa atggaagaac gatatgaaaa attcgcggca accggagtaa
     6961 gaaatatttc tggttacaat gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac
     7021 atccaacctt accattcatc gtagtgatcg tagatgaatt agctgatttg atgatggtag
     7081 ccagcaatga agttgaagat gcgattattc gtttagcgca aatggctcgg gcagcgggga
     7141 ttcatatgat cctagctact caacgtccaa gtgtggatgt tatcacagga atcatcaaag
     7201 caaatgtacc ttccagaatg gcatttgcgg tatcaagcgg tacagattct cggacgatca
     7261 ttgattctaa tggggcagaa aagctcttag ggcgaggaga tatgctattc ttgccaatgg
     7321 gtgagaataa accgatccgt gtgcaaggag cattcatttc tgaccacgaa gtagaacggg
     7381 tcgttcaatt tgttacagat cagcaagaag ctcattatga agagaaaatg atgccgacag
     7441 atgaagttga aacgacaggt gctccagaac agccacaaga tgagctgttt gaagaagcaa
     7501 aagcacttgt cgttgaaatg caaacggcaa gtatttccct tctccaaaga agatttcgga
     7561 tcggttacaa ccgagcagca cgtttagtag atgaactgga agctcatgga gttgttggcc
     7621 cgtcagaagg aagcaagcct cggaaagtat tcattgagca ggaagaaaca tcagaaacgt
     7681 tgccggacga taatgacatg taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa
     7741 taaaaagaca aggcatgatg ataccaaaag aaaaaatggt ttttttccat cttttctaat
     7801 acggtatgat gtcttgtctt tttagttttt gtgagattaa agttctattc gatttatttg
     7861 tgaagtattg cattaaggaa atgaatcatg tatagtatgt agcgaaactt tgagaaaaag
     7921 agggaaaata ctttggaaaa acagtccatt tatggcttga cgaatgaaga attaatcaat
     7981 tggtttatag aaaacggcga gaaaaaattc cgagctgccc aagtttggga atggttgtat
     8041 caaaaacgtg taagtaattt cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa
     8101 ttatcggagc attttatcat caatcctcta aaacagatgg tcgtccaaga agcttcggat
     8161 ggtacagtaa aatatttgtt tgaactccct gataaaaaca tgattgagac tgtactgatg
     8221 agacaagaat atggcttgtc agtttgtgtg acgacacaag taggatgtaa tatcggctgt
     8281 acgttttgtg caagcgggct gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt
     8341 gcacagatca tgatggtcca acattatttt gatgagcgca atcttggcga acgcgtttca
     8401 catgtcgttg taatggggat cggtgagcca tttgataatt acgacaatgt aatggacttt
     8461 ttgcatatca tcaatgatgc caaaggattg gcaattggtg ctcggcacat tacagtttcc
     8521 actagtggat tagcacacaa aatcaaggaa ttcgctaata acggcttgca agtcaatctg
     8581 gctatttcct tgcacgcgcc gaataatgag gtgcgaacat cgatcatgcg gatcaaccga
     8641 agtttcccaa ttgagaaatt gatggaagct gtggatgagt atttagagaa aacaaaccgt
     8701 cgaatcacgt ttgaatatat catgttgaat caagtaaatg atagaccaga gcatgcgcaa
     8761 caattagcag atctattaaa agataagaaa aaattagcat atgtcaactt gattccatat
     8821 aatccagtca gtgaacatga tcaatattct cgcagtccaa aagctgatgt attgagattt
     8881 tatgatgtat tgaagaaaaa cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat
     8941 attgatgcag cttgcggaca attgcgaagc aagcaaatga aaaaaacagt tgccaaataa
     9001 tcgtatccta ctcgtttttt caattaccat aaaaggagca gttttcttaa ttaaggaact
     9061 gttcaactta aataagagaa aagattctat agcgaaagct ataaagcttt ttttgacaag
     9121 aaaaaccctc atcaagcaaa gaaatgtcct caatttgatg acagcctgct tgatgggggt
     9181 tttagcaatt atttaactga ttttcttata tccttgctgg ctttcttgtg tagcatgata
     9241 agtttctacg tcaagaatgg cctgtttaaa tttttctcgg gtgatttcat aaggcacatg
     9301 tttaatatca tttgttgcca tggctttatc taagattgct tctaaatcgg tcgtgacatt
     9361 gagattcatg tcggttaacg atactggaag ttggttcttt ttcataaaca catagatttt
     9421 atcaaacaaa tcgaactgct gatccatcat caataaggtc agtacaccat aactgacaga
     9481 tgctccgtga aggtatccct ccattttttc tagaacagtc gtaccgtaat agaatgagtg
     9541 tgccatagca gaattatagt tattgttaac taaaacagaa accaatccgg tagtaacaat
     9601 gatatccgaa acgacttgtt caattgcata tgagggacga tcgtcggcac tatctttcaa
     9661 tgcttgcgta ccataagtca agagacgatc aatgcacatt tttccgattt ggattcctaa
     9721 tctatccgtg tagcctagat catcgccacg agcagaaaaa gtagattcga cttctttgga
     9781 taaagcatcg ccaatacctg cccataagta tttatcaggt gcttcggcaa taattgctgt
     9841 atcaatgaag gtgtgtacag gtggttttga aggaaagaag agtccgtcca ttacgccttc
     9901 ttcgttatag atgacacata ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa
     9961 ggtgaagact ggtttagcta gtttttcacc aatgactttt acagtatcgc aagcacggcc
    10021 accaccgact gcaaacagga cgtctgcttt ttggacactt tcatgctgga tcaactgttc
    10081 aatatttcca taagttgaat tacccccgta ccagagactg gcgacaacct ctatttcctg
    10141 tgtcaaagct acttttattt tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag
    10201 cactactttt gtcccatatt ctttgacgat tccatttgct ttttcatacg catctgttcc
    10261 aatggtataa tttggtaaga aaacactttg actcatattc atttccttct ttcttcgttg
    10321 aaacgggtta aaattttaaa gagtgatcga tctgactgcg gctgtagatg gagtagaggt
    10381 gaacctaaga caggataagt gatgaataag gtggcatgaa caccttgaaa tcatcaataa
    10441 gtatcctaaa aacgaacaag tacaaaaaca ttgaaaacga caataatgaa tttttgttcc
    10501 tcttagtatt tctaccgatt ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt
    10561 aagattatta tatgaaaaca ggttttattt gtcaattcat aattggtttt ttttaaagca
    10621 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct
    10681 gatctttcct attttgaata aagtgtttct gtataatttt tttattttat aagtaagata
    10741 aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg
    10801 ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt
    10861 gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact
    10921 tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa
    10981 cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct
    11041 caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag
    11101 aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga
    11161 atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac
    11221 ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga acatccgtat
    11281 gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg
    11341 gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa
    11401 aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac
    11461 ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg
    11521 taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
    11581 gctttctaca tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt
    11641 ttattcagat aagggactgt gacaagactt ttgtcacagt ttctttttgt ttattctttt
    11701 ttatctttgt atggcaataa tagacaatat aacgaaaatt atttaatggc ttttggaaga
    11761 ccatggaaaa ccttgtacaa agctattttt tctgaaaaat tccttgaaac ctcacgaaaa
    11821 aatcattatt ttatgaaaaa gacaaaaaaa agtgttgatt ttttaataat tattcattaa
    11881 actcttaaac aacttcagaa aatattaatt attctgaaaa ttccaataaa ggggtacgta
    11941 aaatgaaaaa gaaatttaca tttggtttgg ttgcggtatg cggccttgta cttgctggat
    12001 gttatggcgg cagttcagcc acaagcagta gttcagcaaa aagcggttca gcagatggag
    12061 gaggcgtatt taacttagtc gtgcctcaag aaatgcctac agctgactta tcagttgcaa
    12121 cagatacaat cagttttaca gcattgaata acgtttatga aggaatttac cgtcttgatg
    12181 aagacagcaa accacagccg gcaggagcta gcgagttggc agaagtcagt gaagacggat
    12241 taacttacaa aatcaaatta cgagaagacg caaaatggtc aaatggtgat cctgttacag
    12301 cagcggatta cgtttatggc tggcaacgaa cagtagaccc agctacagct tctgaatatg
    12361 cttacctgta tgcgccagtt gaaaatgcag aagacattac agctggaaag aaagataaat
    12421 cagaactagg gatcaaagca gttggtgact atgaattaga aatcaaattg acgaaacaaa
    12481 caccatattt ccaatatctg cttgctttcc catcattttt cccacaaaaa caatccgtag
    12541 tggaagaaaa tggggatgcc tatgcatctg caagtgataa agctgtttat aatggaccat
    12601 tcacattaga agattttgat ggaccaggaa cagatacaga atggacttat aagaaaaatg
    12661 atcattactg ggacaaagat acagtaaaac tttctgaaat caaagtcagt gtagtaaaag
    12721 aatcttctac tgcattgaac ttattcaaag atggccaagc agatgacgtg atcttgtctg
    12781 gtgagttagc acagcaaaac gcaaacgatc cagcttatac ttctgttaaa gaagcgcgga
    12841 caagctatat cgaattcaat caacgtgaaa agaattcacc attcaacaat gtgaacctaa
    12901 gaaaagctat ttcttattct atcgatcgtg aagctttagt aaaacaagta ctaggtgacg
    12961 gatcagttgt ctctacagga ttgatcccat cagatatggt aaaaagtcca acaacgaacg
    13021 aagactttgc aaaagaagct ggaaaattag tttcttatga caaagataaa gcaaaagaat
    13081 attgggaaaa agcgaaaaaa gaattgggcg ttgattcttt agaatttgat ttgatggctt
    13141 ctgatgatga ttcatcgaaa aaagtaatcg aatatgttca aaactcaatc caagaaaact
    13201 tggatggcgt aacagtcaaa ccaacacctg ttccattctc tgtacgtctt gaccgctcaa
    13261 cttcgggtga ttttgacgca gtactaggcg gatgggctgc tgactatgca gacccaagca
    13321 gctttactga tttgtttgtc acaggtaatt catacaaccg tggtcgctgg agcaatgcag
    13381 aatatgacga agcagttaaa gcagcttcat caacagacgc tggcgatgaa caagcccgtt
    13441 gggatgattt ccaaaaagca atcaagatca tctctgatga tatgggtgtc gttccggtgt
    13501 accaaaaagc agaaggtcac ctagtgaatg aaaaaatcaa aggtatcgtt caccatgcgg
    13561 caggcgcttc atgggattac aaatggacat acgttgaaga ataatcgtta ataccgtgag
    13621 catgagaaaa aacgaggctg cggcaaatgt cgcaacctcg ttctcatatt cacttatcct
    13681 aacaaagaaa aaagcatgaa aatagcagaa agagcaatta gtattccggc aatgatcagc
    13741 caaaagctgt acataccgta gctggcagac gagagcgagg aaaattctgg ttttttcttt
    13801 cggttccttg ttcgtgcaag atggaccgat cgctgaaaat gatcgaagaa acccgaagaa
    13861 aagacccata aaaaaccgcc gataatcaga aacggcaatg accataagaa tagatcatta
    13921 gataattgaa tgagtgtaaa ggaatggttg atgagatttt ttattaaaat aaccagaatc
    13981 agtagaaaag ctactgcgta ggggatagat ttttttttca tttgattgct cctttatttt
    14041 tggttcatta tctaataatg tagcgaaaca tctgtcagaa gtcaaaagct agggggaaac
    14101 attagtgaat agttatatca aatatgtatt aaaacgtgtc tttttcatga tcatcacgct
    14161 ttggttaatt gcaacaatca cctttttctt gatgcaattg ctgccgggca cgccatatac
    14221 gaatcaggaa cgtttgagtc ctgaaacaat cgagatgcta aataaacaag tcggtttgga
    14281 taaaccagtt atcgtacaat atgggattta tctttctaat ttgcttcaag gagattttgg
    14341 aatttccttc caatttaaaa accagccagt cgcacatttg ttagctggaa gaatcggacc
    14401 atccttgcag ttagggttac aggcaattat ctttggaaca gtcctaggga cgattttagg
    14461 aacgatctct gccatgaagc aaaatacatg ggcagataca tcttctacac tcgtagcgat
    14521 attaggtcgt tccattccaa actttgtatt tgctgtttta ttacaatata tctttgcaat
    14581 caaattacaa gttttgccaa tcgcaaaatg ggatggcttt gtgtacacaa tcttaccaac
    14641 aattgcgttg gccatgtcgc cgcttgctga ctctgctcgg tttatccgta cggaaatggt
    14701 ggaagtactg catagtgatt atgtggaatt agcaaaagca aaaggattga gccgttggga
    14761 aatcgccttt aaacatggtc ttcgaaatag tttgattcca ttgatgactt tattaggacc
    14821 gttagctgta gcattgatga ccggatcact ggtggtagaa aatatctttg ctattcctgg
    14881 tatcggggaa caattcgtta agtcaatcac aacaaatgat tatcctacga tcatggctgt
    14941 aacgatcctt tactcattta tgctgatttt tgttattttg atcgtggatc tattgtacgg
    15001 tctagttgat ccaagaattc gtgtttcaga agggagccga ggataaatgg aaatgatacc
    15061 aaagcgttat gaaacagttg cagatatccc tgcggatgaa tttcttccat tacaaaaaaa
    15121 taccgaagaa gaacgtgaac agattactgc accttctttg aattttatcc aagattcatg
    15181 gcgccgttta aagaaaaaca aagctgccgt tatttcaatg gttttactat tggtcatcat
    15241 tttgatctct gtgatcacaa tctttgtttc tcctcatgat ccaactgccc aaaacgtggc
    15301 ttatatcaat ttgcctcctc gtattccggg aatcaatatc gatggattga atggaaaagc
    15361 aatggttggt ggtgaacttg ttgataaata tgctcaagca aacgtccctt caaacgtaaa
    15421 tttcttttta ggaacagacg gtttgggacg tgatgtatta agccgcttat ttatgggaat
    15481 gagaatctcg ttgctgatcg catttattgc tgcattacta gatgtaacga ttggtgtagc
    15541 ttacgggttg gtttctggtt tgttaggcgg acgtgtcgat aacgtgatgc aacgtttttt
    15601 ggaaatctta tcaggaattc caaacctcgt tgtcatgatt ttgatgttgg tcgtttttga
    15661 accaggaatt ttttcaatcg tggctgctat ggccatcaca aactggatac caatggcacg
    15721 tatcgttcgt gcacaaacaa tgaaattaaa agatcaagaa tatgttttag ctggattgac
    15781 attaggtgaa tcaagaatga aaattgcatt caaacatatt ttgccgaata tctctagtgt
    15841 cattattatt caaatgatgt ttagtattcc atctgctatt ttcttcgaag cctttttaag
    15901 ctttatcggt ttaggtctta cgcctccgtc agcttcatta ggaacgatgt tgagcgaagg
    15961 gtacaagaca ttcctgtatc tgccttatct attatggatt ccggcagtaa ctttatcggt
    16021 tatcatgatt ggctttaact tacttgctga tggattgcgt gatgcctttg atcctaaaat
    16081 gaaagagtga agacgatgac gaaaatttta gaagtgaaag acttagaaat ctcttttgat
    16141 acgtatgctg gaaaggtacg agcgattcgc ggtgtcaatt ttcatttgaa taaaggagaa
    16201 acattggcga tcgttgggga atctggaagt ggaaaatcag tgacgaccag aagcatcatg
    16261 cgcttgctgt caagcaacgc caatatagat gctggtcaaa tcctctttaa aggtaacgat
    16321 atcgttggaa aatcagaaag agagatgcaa aaaatccgtg gtaaagaaat agcgatgatt
    16381 ttccaagatc caatgacctc tttagatccg acgatgccca ttgggaagca agtcgctgag
    16441 tcccttagaa agcataataa agtttcaaaa aaagagggac aaaaagctgc attagaattg
    16501 cttaaattag ttggaatacc cgaagctgag aaacgtatca acagttatcc gcatcagttc
    16561 tcaggaggtc agcgtcaacg gatcgtgatc gcgattgcac tggtatgtta tccggaaatc
    16621 ctgattgctg atgaacctac aacagcgctt gatgtgacta ttcaagcaca gattttggag
    16681 ctgctgaaag aaattcagac aaaaatcgat acatcaatta tctttatcac tcatgacctt
    16741 ggtgtagtag cgaatgtcgc tgatcgtgtc gctgtcatgt atggaggaag aatcgttgaa
    16801 gttggaacat cggaagaaat tttctacaac ccgcaacatc cctatacttg gggactgctt
    16861 ggttcaatgc cgacattaga tagtgcgaac gatcgattgt atgctattcc tggttctcca
    16921 ccggatttat tgaacccgcc aaaaggagac gcattctatc caagaaatga attcgcgatg
    16981 aaaatcgatg cagaacaaga accaccattt tttgaactat caaaaacaca caaggcagca
    17041 acttggctac ttgctccgca ggcacccaaa gtgacaccgc cagaagaaat ccaacgtcgc
    17101 tgggctatct ttaaagagaa acaaaaacat acttacggtc aaggaggaat tgctgatgcc
    17161 aaataaacta cttgaagtta aaggattgaa acagtatttc aatgtaggaa aaaagaatga
    17221 agtacatgct gtaaatgaca tcagctttca catttatgaa ggagaaacat tcggtttagt
    17281 tggagaatct ggaagcggaa aatccactac tggccgtacg atcattcgct tgaatgaacc
    17341 tactgatggt gagatattat tcgacggtca agatataacg aagatcaaag ataaaaaagg
    17401 tttgacgaaa ttccggcatg atgttcagat gatctttcaa gatccatacg cttcgttgaa
    17461 tcctcgtatg aaagtcagag atatcattgc cgaaggaatt gatgttaatg gactagctaa
    17521 atcatctgaa gagcgtgcaa aaaaagtcga tgatctattg agaactgttg gattgaatcc
    17581 aagccacggt actcgttatc ctcatgaatt ttcaggtggg caacgccagc gtattggtat
    17641 tgcacgagca ttggcagtaa aaccgcgttt tatcatttgt gatgaaccaa tttctgcact
    17701 agatgtgtcg atccaagccc aagtggtcaa tttgttgcaa gatctccaaa aagaacatca
    17761 attgacctat ctatttattg cacatgattt gtcgatggtt aaacatatta gtgaccgaat
    17821 cggtgtaatg cataacggct tgttattaga gatgggaaca agtgatgaaa tctataatca
    17881 cggtgttcat ccttatacag aaagtttgtt atctgctatt cctctaccag atccagacca
    17941 tgagagaaaa cgcagaagaa tcaaatatca acctgaacca gatgacggac aagtacgtca
    18001 acttcgtgaa atcgcaccag aacattttgt ctatgcaact gagcaggaag tcccatatta
    18061 cgcaaaaaaa ctaaaacgac aaaaggaatc tcttctggtt gctaactagg agggacatag
    18121 atgctaaaag cttttaaatt ccgtatgtat ccgacagaag aacaaaaaca gcaattgatt
    18181 cgtacttttg gatgtgctcg atttacctat aaccatttgc tgaaaaagcg gcagaaaagt
    18241 tggcaacaaa caggagtagc caatttttcg ctcacacccg ctacattaaa aaaagaatac
    18301 ccattcttaa aagaagtaga cagtcttgct ttagcaaatg cacagcttaa tttggatcgg
    18361 gcttttcgca attatttcaa gggacgtgcc agcttcccaa agcttaaaac gaaaaaaagt
    18421 atgtggcaat cttatacgac caacaaccaa acccgtacga tctatctgga aaatggctac
    18481 ttaaagttac caaaacaaaa agaactaatc aagataaatc agcatcggcc agttgaagga
    18541 tcgattcgct ccgctacgat ttctgctcga tataatgaag aattttatgt cgcactactt
    18601 tgtgatgttt cgccagtaaa aaaagaatct ttagctaaat ggatcgggat cgcctatcat
    18661 ccgaagacat tgatccaaac atctcgacct ctagaagtaa ctttaccaaa atttcaccag
    18721 acagaagaaa agctgcaaca tgcacagcgg aaattgaatg tgaaagtccg ctccgctcac
    18781 catagaaaga tcaggttaga tcaggcaagc aattatcaga aacaaaagag aaaagtaatg
    18841 gatctttatc tgaaacaaaa gaaccagcga gaagattatc ttgaacaatt atctgggaaa
    18901 ttggtcaaac aatatgacta cttatttgta gaatcttttc caaaagaaga ggcacatgct
    18961 gatttttcta ttcacgactg gcataaatta ataacaaaat tgcgttataa gtcccaatgg
    19021 tataataaaa aatttctttt gatcaatacc gacggagcag aagaaagcaa ttccgtaaga
    19081 aagagccaag tagtagaaaa aatgggcaga cattcggtta tcaagggata aaaaatgaat
    19141 gaaactgata ggagcgttag ggttagcctt gttaataaaa agtcttaatg acttttgttt
    19201 ctaggaattt ttcttttgaa gaaaagctca ctaattatga caaaccaata aaacaatagc
    19261 agaaaaagtt cgtacgagct ttttctgcta ttgttaattt tttattcaga aagtttacga
    19321 atacatcaaa attctgaaac aaaaaacgta actcacaaag agttgttttt gcgttttttc
    19381 ttgaatgatt aggtctgaat cttttgctca caaccttatt catggtgccc cggcttgagc
    19441 tgatagattt ttgaaggacg gccgattcct acaggacgtt caccgatctc atcgaaatat
    19501 tggagcatag ctttcttaaa attggaatga tcgatcgttt tataatcgat ccctaagaat
    19561 ttagcaaata cttttcgagc ttccgtaatc gtgaaatctt tacctaaaac ttgtagaact
    19621 tgcggctcgt gttccatttt attgactaca cggttgaatg ctttcaagat gatttgacta
    19681 tggtcaaaag ccaaagtatc tttgccagaa gactctccag tgactaaatc aagcgagata
    19741 gccacttctc cgctagataa atggatcttg ttactatgac gttctagtgt gaaccagcgt
    19801 acttccttgg catcatctcc ggcgattagc ggttcttctc caataaaggc taagtagctg
    19861 actgtgacga cccagcctcg cggatctcga ttaggcgtac tgaatgtatg cagttgctca
    19921 atattttctt ttgaaataac cacaccagtt tcttcctttg tttctcttaa gacgctttct
    19981 ccagttgact catccttttg gacaaagcca ccaggcaatg cccatgaatt tcgataagga
    20041 tgcccttttc tttggatcaa caaaactttc agctgatctt cttctttgtt atagcacatc
    20101 agtacgatat cgacagtcaa agagggcttg tcatattctg gacgttcctg tttttgatac
    20161 cagttaagaa attcttcttc acttgcttta tgttcataat ataatttttc ttcttcttta
    20221 gaagcaaaac ggttcatctg accacctcca ttgacagtaa aagtactttt taccttattg
    20281 tagcagactc tgaaaaaaaa ccaaggattt acttgaaaaa tctaggttat catttaaata
    20341 tgaaaaataa atggaagtat gagattgaat aaaaagaaaa gcagataaaa cggcaatact
    20401 ttataccgct cttcacgaat ggcaaagaag ccacaaataa cgatatatac gtcaatgata
    20461 aaacgcaaaa tccaagctgc tgtcataaaa ctgttcaaat gcccatggag gttaaaataa
    20521 ccatacatac gaatataaaa atagctgaat attaaatgtg ctaatgaaag aaaagcaata
    20581 ctaagtgtaa aaaaaagatg tttcttctgc atgattgttc cagtccctta ctaagataga
    20641 tagtctttat tataccacgt gtgaggggag aaataaaaag ccgtttttta gtagatatga
    20701 aacaaaacaa aaacccgcat ttattcaacc tattcatcct tttaaaggat agattgaacg
    20761 tagatagaag tcgtttatcc agtctttttc attttgaaaa aaagctaaca gcatgctttt
    20821 tttatggtat actagcaagg aaacgaattc gcatcatgcg ataaaggagg aagagccccg
    20881 tggcagaaaa ggaaactttt tatatcacta cgccaatcta ttatccaagc ggcaaacttc
    20941 atatcggcaa ctcgtatact acgattgcat gtgacgcaat tgctcgttat aaacggttga
    21001 tgggctttga tgtgttttat ctcactggag tggatgaaca tggtcaaaaa atcgaaacaa
    21061 aagcagaaga actaggtgta aaaccgcaag aatatgtaga taaaatggcg gctgatgtca
    21121 agaaattatg gaaaacgctt gatatcagtt atgataaatt cattcgcacg acagatgatt
    21181 accataaagc agctgttcaa aaaatatttg atcgtttatt agaacaggga gatatttact
    21241 taggtgaata tgaaggctgg tactctgttt ctgacgaaga attctttaca gaaacacaac
    21301 ttgcagaagt gtacaaagat gacgaaggta acgtgatcgg tggtaaagcg ccaagtggac
    21361 acgaagtaga attggtaaaa gaagaatcat acttcttccg catgagcaaa tacgcagacc
    21421 gtctattgga atactatgaa gaacatcccg aattcatcca gccagaatct cgtaaaaacg
    21481 aaatgatcaa caactttatc aaaccaggat tagaggatct tgcagtttca cgaacaactt
    21541 ttacttgggg agtaccagtc aagagtgatc cgaaacatgt ggtgtatgta tggatcgatg
    21601 cattatccaa ttatattact gcactaggat atggctccga agatgaaagt ctattcgaaa
    21661 aatactggcc tgcagatgtt cagatggtcg gtaaagaaat cgttcgcttt catacgatct
    21721 attggccaat catgctgatg gcccttgatt tgcctctacc taaaaaagta tttgggcatg
    21781 gctggctatt gatgaaagac ggaaaaatgt ctaaatcaaa aggaaatgtc gtttatcctg
    21841 aaatgcttgt ggaacgttat ggtttagatg cactccgtta ttatttgctt cgtgctgttc
    21901 cgtttgggtc agatggtgtc tttacgccag aagattttgt ttcccgtgtc aattatgatc
    21961 tggcaaatga tctagggaac ttattgaatc gaacgattgc tatgatcaac aaatattgtg
    22021 atggactcgt tcctgactat gcctcacttg tcactccatt tgatagtgaa ctgtctacga
    22081 cggctgctaa cgttgtcgga cgttaccatg atgcaatgga aaaaatggaa ttcaatacag
    22141 cattggctga aatctgggtg ctgatttctc gtgcaaacaa atacattgat gaaacagaac
    22201 catgggtatt agcaaaagac gaagaaaaga aagctgaatt gaatagtgtc atgatccatt
    22261 tagctgaatc tttgcgtatc gcagcgatat tacttcagcc aatcatgaca gaaacgccag
    22321 agaagatctt caatcaacta ggattggact ctgaaacaat gaatcttgaa ggattacact
    22381 ttggcgaatt tccttcagga acaaaagtag ttgcaaaagg tacaccaatc ttcccacgtt
    22441 tagatatgga agaagaagtt gcttttattc aagaaaaaat gtctgaaggt actcaaacaa
    22501 acgaagatac agtcaaatgg gatccagaag aaacagaact tgtttcaacg aaagaaaaac
    22561 aaatcaaatt tgatgtattt gaaaaagtag aactaaaagt agcagaagtc atcaactgtc
    22621 aaaaagtaga aggggcagat aagttgctgc aattccgact agatgctgga gacagccaag
    22681 accgtcaaat cttgtcagga atcgctgaat tttatcctga tccaagcgaa ttgatcggga
    22741 aaaaagtcgt cattgtagca aatctgaaac cacgaaagat gcgtggacaa atcagtcaag
    22801 gaatgatcct ttctgcagaa gcaccagatg gctctttaca agtaatcgaa gcaccaaaat
    22861 ccatgccaaa tggctcagaa atcgcataga aattcaaagg gggtatgaca ctagttgtgt
    22921 cataccccct ttttccgaaa tttgagaagc aattttcgta aattccttct tattacttga
    22981 agccaatcat ataatacaga aacagagaac tatccaaaat aaatcctaga aaaagaagta
    23041 cgagcttcgc tgatttttgt taatttagaa taatactaat aggaatcgta aaaacagaaa
    23101 aaaagcataa tgataaaggg tcgggcagat tttaattgat tcatctggta ctctttttta
    23161 ttgtccttac attcataaag tttgaatgca atgactttat tacatctata tttatatcta
    23221 acagtagcgt tgatgtgtta attataagtt ctatcacttt atgttcaatt agtttgtgct
    23281 cttatatgtg tgattgaaaa tatagataaa aaaacaatca tcggctttga cttcgatgat
    23341 tgttttcttt attttgtaaa aatcctatca cttgatttta tggtaacctt ttatttttgt
    23401 cttcaaacgg cttggtaaga gagcagtaag cagtgcaaat gggatcgaaa agacatacca
    23461 gaaaatcgta cgtccgatac cataatgttg atagagcttt gtcaacatca cgtgtaacaa
    23521 gatcaaccct aaaagcaagg agccagctat aaaccaatga ttatcccaat gcatgacttc
    23581 actaagtgcc atcactccaa aaggatagaa ataaaaatag atcccgacta aggtcatccg
    23641 ccagttcttt gtaaatgttc cgatattcaa taaaaagaaa atcgctgaaa gcaaaatacc
    23701 gataggcgga aaaagcccaa ccaaaaagga atacaacatc ccccacaaga tcatcacagg
    23761 acctttgatc aatgcaacga ttgcgatgaa caaagtcagc aatattagtt tttgtgataa
    23821 cgttgcaaca gtcattaatc caaataaaat taatgtgaac caatgccaat gggactgctg
    23881 ttgttcttta cgggaaaaat cgacccggtt ggataaataa tctgctaatt tcggataaga
    23941 acgcttaaaa tccataaact gttcctcctt cgtggaaaag gtaggtttag tatacgaaaa
    24001 ataaaaaaga aatacatccg tcttaagagg gagtttttca ctgaaagtgg agaaaaaaga
    24061 atggtaaatt aaaatattct cagttatact gaaaattata ccaaatttca aagaagaaag
    24121 aggatgggaa tgaaaggcaa actaaaacga gaaatcaatc tgtttggcgc attagctacc
    24181 gttatgggaa ccgtgatcgg tgcaggcgtc ttcttcaaaa ctgctgctgt tactgctagt
    24241 actcagtcta caagtttgac attgctagct tggcttttgg gtggtttctt aacgatctgt
    24301 gctggattga cagtggctga attagctaca gcaatcccgg aaactggtgg agctgtaaaa
    24361 tatattgaag ctgcgtatgg aaaattgccc agctttttat taggatgggc gcaaagcttg
    24421 atttactttc ctgctaatat tgctgcatta tcgattatct ttgcgacaca attgacgaac
    24481 ttattacaat tatctaccga ttatcttttg ttgattgcag tgatcaccgc tgtttctgtg
    24541 acagggttga atttgttagg gacaaaagtc ggtgcttctg tacaatcagt gacgttgatt
    24601 gttaaattaa taccaattgc tgtgattgtc atatggggat tattgacccc aggacaagga
    24661 actgtccagt tatttccaat cgaagctgga aaagatgtta cttttgtcga aggattgagc
    24721 agtgcgttgt tagcaacgtt gtttgcttat gacggctggt taggtgtcgg tgcaatggca
    24781 ggggaaatga aacggccgga aaaagatttg cctaaagcaa ttattcttgg tttgagtttt
    24841 gtaacagtcg tttatctatt gatcaatttt gtttttttga aaactttgcc gattgatcat
    24901 cttgccggta atttgaatgc agcttctgaa gcatcagacg tcatttttgg aggaatcggt
    24961 ggaaagctgg tcactattgg gatcttgatc tcggtatacg gtgcattgaa cggttataca
    25021 ctaactggga tccgcgtgcc ttatgcgatg gctttagaag acgagctacc tttcagtaaa
    25081 cagctgacaa agctttcaaa gaagtttacg gttccttatg ttcctgcggt tttccagtta
    25141 gcagtagctt gtattatgat gagccttggt tctttcgact ttttaacaga catgttgatt
    25201 tttgtgatgt ggctattcag tttactgatt tttatcggtg tctttatttt aagaaaaaaa
    25261 gcgccggaat tgccacgtcc atataaagta ccactttatc caatcgttcc gattattgcg
    25321 attctcggtg ctattttcat tttaggtatg acgatgatga cgcaaacgaa attagcattg
    25381 atcggtatag gtgttacctt gataggtatc ccagtctatt atcaaaaaaa gaaaaaacgc
    25441 tcagaagagt aaaatatttc aacaacagac agaaaaagta cgaactaggt cctctagatc
    25501 ttcgtgcttt ttctatttca ataaaacaag ttcttctatt ttgatttttg ctacttctgc
    25561 aagtagtgct tatgtgctaa agtaagaatg aaaaggagag ataaacatga tatttgattc
    25621 tcatacccat ttaaatgctg aacagttcaa tgaagatatt ccagaaacga ttcagcgagc
    25681 acaagaaatg ggggtaacga aaatggcggt cgtgggcttc gatacgccaa cgattgaaaa
    25741 gtctttgcaa ttaagccatg actatccaaa tatatacagt attatcggct ggcatccgac
    25801 agaggctggc agttatacga aagaaatcga aaataaatta caagaacaat tgactttacc
    25861 aaaggttgta gcattagggg aaattggctt ggattatcat tggatggaag acccgaaaga
    25921 agtccaagat aaagtatttc gcagacagat tgctatcgca aaagaaatga acctaccaat
    25981 cagtattcat actagagaag cacttgaaga cacgtatcaa atcctaaaag aagaagatgt
    26041 tcgagatatc ggtggaatca tgcatagttt cagtggtgat tatgaatggg caaaacgatt
    26101 tcttgactta gggatgcata tttcatttag cggtgtcgtc actttcaaaa aagcattaga
    26161 cgtccaagag gctgcagcaa atgtaccaat cgaacgatta cttgtggaaa cagatgcccc
    26221 ttatctggca ccagttcctt ttagaggaaa gcgaaatgaa ccaggctata ctcgttatac
    26281 agtagaaaag attgctgaac ttcggaatct tccgacagaa gaagtggctg cccaaacatg
    26341 gacaaatgcg caccgattat ttggtctaag cgaacatgga taagatgaag atccaagaga
    26401 ttatagtcgt tgaaggaaaa gacgatacaa gaaggctgcg ggaagtagtt gatgcagata
    26461 cgatagaaac aatagggtct gcaatcaatg aagagatcct gatgcaaatt gaacatgccc
    26521 aagagactcg tggagtgatc gtttttaccg atcctgattt ttcaggagaa aaaataagaa
    26581 aaatcattat ggaagctgtt cctcaggcaa aacatgcttt tttgccgcgc acacaagcta
    26641 aaccaaagaa aaagggaagc cttggtgtcg agcatgcaag tgatgctgct attttagaag
    26701 cattgaaaaa ggtcgtgacg ccagacattt cccaaacggc tgtttctgaa atcactagac
    26761 aagacctttt agactatggt ttaatagctg gggcacaagc aaaagaaaaa cgagaaaaac
    26821 taggtgacga gttgcgcatc ggttacacga acggcaaaca attggaaaaa cgtttgaaga
    26881 tgtttcgtat tacgccgaaa gaactcgccc aagcaatgaa aatagtggag gaacaacatg
    26941 agtgaatatc gtgaaattgc gacaccctct agaacaaaag agattttatt gaaacatggt
    27001 ttttcattta aaaaaagttt aggacaaaat tttctgactg aaccaaatat tttaaggaaa
    27061 atcgtagaaa ctgcgcaaat agatgaacat acgaacgtga tcgaagttgg accaggaatc
    27121 ggtgcattga cggaacaatt agccaagcat gcaaaacaag tcgtagcatt tgagatcgat
    27181 gatcgtttga tccctgtatt ggctgatacg atgcaaccct atgacaatgt gactattgta
    27241 catcaagata ttttaaagac cgatttgtca acagctgtta gagaaacatt tcatgaggag
    27301 cttccgttaa aagtcgtagc gaatttacct tactatatta cgacaccgat catgatgcat
    27361 tttcttgaat cagatttggt tgtggatgaa ctagtcgtca tgatgcaaaa agaagtagct
    27421 gatcgtatct ctgctgaacc aggaacgaaa gcttatggtt cattatcgat tgctgttcaa
    27481 tattatatgg aagccagttt agcattcatc gtaccaaaaa cagtatttgt ccctcagcca
    27541 aatgtagatt cagcgatttt gaaactgaca agaagagata taccggcagt cgaagtgaca
    27601 gacgaaaaag catttttccg attgacaaaa gcggcatttc agcaaagaag aaaaacgctt
    27661 tggaataact tgcagcattc gtatggcaaa gatgaccaga caaaagcatg gttagcaaaa
    27721 agcttggaaa cagctggaat cgatccaaaa cgtcgtgggg agactttgtc attacaagaa
    27781 ttcgccgcat taagcaatgc gatgtcagaa aatatgcagt aaaaaaagag gttgtgacag
    27841 aagttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    27901 catgtttttc gtttgtttgg gcttgttacc gcagatgcaa gcttttgaac accgtttaag
    27961 aaagaggttg tgggcctgcc gtttaactct aagtcacaag gaacaatttc aaaaaacagc
    28021 ttctcatgtt tttgagttag ttggtcttat tacttgatgc aagcttttga ccactattat
    28081 gccgaacaag gagatgtgac acaaattttg tcacatctcc tatttttgtt tcttgatcaa
    28141 aaaattctta tacttgatac tcacactcat tttgtatgga tttagaaaag aatatgtgtc
    28201 ttgacagtta ttagtaagtg agttatacta agtcgacatt tatgtgtcaa gacatattag
    28261 gagagagaaa aaatgagaaa aaattcagtt cgaaaactaa cgatttctgc tttattgatt
    28321 gggatggggg tcattatccc aatggtcatg cctaaaatca tgattccacc agcttccttt
    28381 actttggcgt ctcatgtgcc attatttatt gcgatgtttt ttacaccagg agttgcagtg
    28441 gctgttgcgc taggtacgac atttggtttc tttttaacaa cgccggttat catcgcattg
    28501 cgagcattgt cacatcttgt gtttgcgttg attggggctt ggtacttgca aaagcatccg
    28561 agtatcgttt taaaaaatgg acagtttact tttgctaatt ggcgtttcca agggttcaat
    28621 tttgtgatcg gtgtgattca ttctttagca gaaatgctag tagtcagcat cttttacttt
    28681 attggtaata tgcctgaaac atattattct caaggatatt tttatacagt attcatcttg
    28741 atggggattg gtgggcttat ccacagttta gttgattata atattgctta ttttatagca
    28801 ggaacactca gcaaatattt tgatatcccc gtatttacgg cagcaaaaaa agaaaatcgg
    28861 aaagagtcga ttcaagggta cgcgaagaaa acgacatatt agacatgatc tctcaaaaaa
    28921 aaaactctca tctagtaatg gataataaat cctctaagat gagagttttt tgtcctaaaa
    28981 agagctgcac caacatcttc tggcacagct ctaacagggg gcaatttatg gaagtttgat
    29041 gacttgttct gtttctttat caaagaagtg gcctttgctg atgttgaagg caaggtcgac
    29101 tatttcgcct ggtttatgga aatctcttgc atctactttt gagatgaact cagtagaacc
    29161 agtttttgtg taaagcatcg tttcagctcc aagtaattcg gaaacgacaa cttctgattt
    29221 cactacggct tcaggtgaag cttcaagggc tacttgttca ctgtggatgt cttctggacg
    29281 aataccgaag ataacttctt ttccttcata acctttttca actaatactt tgttttttcc
    29341 ttcaggaata cgaagcttaa gcccatgtcc atctgtgata acaccatttt tcaatgtcac
    29401 attaaagaag ttcatggcag gagatccaat aaagccggca acgaacatat tgacgggtgt
    29461 atcataaact tctttcggtg taccgatttg ttggataaat ccgtctttca tgataacgat
    29521 tcggtcagcc atagtcattg cttctgtctg atcgtgagta acatagatgg tcgttgtttc
    29581 aagacgctgg tgaagtttgg caatctcagc acgcatggct acacgtaatt tagcatctaa
    29641 atttgataat ggctcatcca ttaagaagac ttttgcatcc cgaacgattg cccgtcccaa
    29701 tgccacacgt tggcgttgtc caccagataa agcagctggt tttcgtttca agtattctgt
    29761 cagacctaag atctctgctg cattgtccac acgttttttg atgtctgctt tatcatattt
    29821 acgtaatttc aagccaaaag ccatattatc aaaaacagtc atatgaggat acaatgcgta
    29881 gttttggaaa accatggcaa tatcacggtc tttaggtgcc acgtcattca tgacttttcc
    29941 gccgatattt agttctcctt cagagatatc ttccagtccc gcgatcattc gcaaagtagt
    30001 tgactttcca caaccggaag gaccaacaaa aacgataaat tcacggtctt tgattgatag
    30061 attaaaatca gttacagaat aattttctgc attgtcatat tttttatata cgtttttcag
    30121 agccatttct accataatac gttccacact ttctttttta gttttattaa ctatttaaag
    30181 tataaatgaa agcgttaact aacttcaatg tcatgttgaa caagaatttt ttcttttttt
    30241 tgtcaatgtg ctagttttaa gctttaataa atatccataa gaaataaaaa ctgatacact
    30301 ggagtaaata agtatgttaa caatatcaaa aggagtgtct cttatgaaaa tcgctttgat
    30361 cgctcatgac cgaaaaaaac ctatgatggt gaaattagca acagcgtata aaacaatatt
    30421 ggaaaaacat gaactttttg ctaccgggac gactgggcag aggatcacgg aagcaaccgg
    30481 cttgccgatc catcggttta aatccggacc gttaggcggg gatcagcaaa ttggagccat
    30541 gatttcggag gatcagttag atttggtgat ttttcttcga gacccgctgg ctgcacagcc
    30601 ccatgaacca gatgtaacag cacttatccg tttatctgat gtctatgaga ttccgttagc
    30661 tacgaatatc ggaacagctg aggtgctact aagaggactg gaagccggct tcctagattt
    30721 tcgaagaatc gtccatgaaa tagacaagca tccactttca ttttgatgga taagcgtatg
    30781 gaaaaataac gtttaatgtt cgcctatatg tagctataaa agctaaacaa actgataata
    30841 tgcgatataa tacgaacgta ttggataata taaacaataa tattagtttt tcgtatttgc
    30901 agtaaatgtg aaaatatgct atagtttctg tgggaaaaga tcccaaaaat aatacagagt
    30961 agaaaataaa gcgttaagtg ctaagaggat gggatgttgc ctcttggacg aaggacgtaa
    31021 tgttcgcggt tttattttcg cattcgctgc atgatacgat gtagcagctc ttaaaggaga
    31081 tgctaaaaat gaagaaacac cggttagacg cacgtatgat tgctattatg ggcttattga
    31141 ttgcattgat ggtcactttg tcccgactgg tagcaatcga gaccccattt attaaaatca
    31201 gtgtcacatt tattccacaa gtgatcatgg gcattctttt tggtcctttt tggtcaggga
    31261 taggcgcagt tttagctgat ttagtaggaa tggccctctt ctcgaaatct gcattcttta
    31321 ttggatttac attgaatgca tttattgaag gagcaatcta cggattcttt ttctatcgaa
    31381 aagagatcac ttggaaaaat gccattctgg ctacgcttag tgtcacgctg atcatcaact
    31441 tatttttgac accattatgg cttgcgttga tgtaccatgt gccattgttc agctgggtcg
    31501 tttgggcgcc ccgcctgtta aaaacagtaa tatggttacc gattcaatca attgccattt
    31561 attacgtagg acgtagtatt ccttacaaaa aaattttaag aagtttagcg atccacgcta
    31621 aataaaaaac aagcagtctt cccatcccag aagactgctt gtttttattg tgctgtgaat
    31681 tgtgcttgat agagccgtcg gtaataacca tctttttgaa gcagctcatc atgggtgccc
    31741 atttcgacaa tagctccctg atccatcact aatatctggt ccgcattttt gattgtggac
    31801 aggcgatggg caatcacaaa actggttttt ccttccatca ttgctaagaa ggcttgttgg
    31861 atttttttct ctgtcaatgt atcgactgag ctggtagctt catctaagat caacataggg
    31921 ggattactga tcatcgttcg agcaatcgtc aacagttggc gttgtccatc tgatatcttt
    31981 aacccttgac cgccaatctt agtatctaat ttttccggca aacgttctac aaattcatac
    32041 atataagagg cttttagggc atcattgatt tctgcgtcac ttgcttccgg attcccaaaa
    32101 cgaagatttt ctcgcaatgt actgtcaaat agccaagtat cttgtaacac cattccaaaa
    32161 cttttacgta atgtatcacg ttggatagag cggatgtcat ggtcatcgac tgtaattgtt
    32221 ccttttgttg tgtcataaaa acgcatcagc aaattaacaa gtgtggattt tccggcccct
    32281 gtttttccta ctatagcaat ggtttgacct ggataagcag taaaattgaa atcttcgatc
    32341 aaacgctgct tggggtcata agaaaaagaa acatgatcaa aagagacttt tcctttgata
    32401 tttttgggtt ccaaggcata ggcatctggc aaatcaggct tttcttctgg ctgatcgatc
    32461 aaatcaaatg ctcgttctag accagaaaaa gcggtttgca gttgtgtcgt gattccagaa
    32521 agttcgataa atggtttaga aaactgactt gagtagatcg taaagctgga aatcacacca
    32581 acagtgattg acgaaccact cttcaatgcc aataggccgc caactaaacc aatcgataaa
    32641 taagcaaggt gatctacaaa acgagaagac gggtttgtca aggaagaaga aaattgtgcc
    32701 cgttgtcctt tttcatatag atcagcattt agcagttcaa attgctgttg actagcttct
    32761 tcttgctgaa aagctttcac gattttctgg ttaccgattc gttcggtcac aaagcccgag
    32821 atatccccaa taatcctttg ctgtctatcg aaatttgttt gtgaagcacg agcaacgatc
    32881 caactcacaa ggaaaataat tggtgtagaa caaagaacga ccaatgtcag aagtggactt
    32941 agacgcatca taaaaataaa cgaaagaatg atgattgcca ctccagaaaa taattgattg
    33001 aaggccgcag aaattgcaat ggaaatattg tccatatcat ttgtaaatcg actgacgata
    33061 tttccgtggg agttttgatc ataataacga agtggcaatc gattcagatg agcaaatgca
    33121 tcttttctta attttgcaac ggataagtaa gctaaccgat tgcttaacac ttggatgagc
    33181 cattgactga ctacgttgat taaaacgata ccaccgaata gaataagtaa atggaacagt
    33241 cgattgaagc ggacctgtcc ttctccaatc atttgatcga tactttgacc aatttgtaac
    33301 gtcatcacaa ctgttgcaat cccattgatg attcctagta tcacagctag ccaaatgtct
    33361 tttgcatagg cagtaaggta aggaatgaat cggcggagcg tctgcaagga taattttttt
    33421 actttcataa ctattcttcc tcctgtgatg cgacgatttc ctgatattct gctgattgag
    33481 caagtaattc ttcatgggta ccctgagcag caatcttacc ttgatccatc actaagatat
    33541 gatgtgcttc ttgaatagag cggacacgtt gggagataat aatcactgta ctgccaagat
    33601 aagtctgtag cgccttacgc aaatccaagt ctgtttgata atccaacgca cttaaagagt
    33661 catctaaaat cagtaatgct ggcttggcaa tcaaagctct tgcaatcgtc agtcgctgtt
    33721 tttgtccgcc agagaaattt ttcccatttt ccatgacttt tgtatctaaa ccatttggaa
    33781 gctgacggac aaattcttct gcctgagcga ttttcagagc ttcccaacat tctttttctg
    33841 tggcgtcttt cttaccccat tgaaggtttt cacgaatagt cccggtaaat agtacagaag
    33901 tttggggaac aagagcaatt tgctgacgga gatgatccag ctgccaagaa cgaacatcca
    33961 ttccgtccat tataactgta ccggaactga catcatagaa acgcggaatc aattgtgtca
    34021 aagtcgtttt cccgcttccg gttggaccaa tgattccaag gactgtattt cgcttcagct
    34081 gaaatgtaat attttggaga gaaagaccag aatcaggagt ataacggaag tcaacatggt
    34141 caaaagcgat aattgcttca gaagaaaaat cgggagtagt actgacctca gcttcaagaa
    34201 tcgaattttc tgtgtctagc acctctttca cccgatttcc tgaagcttcg gcacgtgtga
    34261 agatcacgac gaggtttgaa acaacgatca gtgccaaaag catttgattc atataattga
    34321 tcaaagcaag aacttgtcct tgttgcaagc taccaatatt tactttgatc ccgccaactg
    34381 tcagcaaaca gatgatcccg acattcatga tcaaggttgt agcaggtgat aatagggcag
    34441 agatattgga aacacgtaaa taattgttcg ctagatcatt tgtgctttta tcgaacttct
    34501 ctgtttcttt ttttgtctgc gcaaatgcgc ggatgacacg aacaccgcta agattctgac
    34561 taacgtgttc attgagctga tctagttttt cttgcacttt ttggtagaga ggaacggttt
    34621 ttttgatgat aaaaaataaa atgattgaga aaataggcaa taatgctaag aaaaacagac
    34681 ctacttccca gtcgatcaca aaagccatga ccactgaccc gatacttaaa aacggtgctc
    34741 tgatcaataa acgaatgacc attgctaaag ctagctgaag ctgattgatg tcgtttgtca
    34801 ttcgggtgat caaggtatct gttccaaaat gattcaactc gttgtgagag aaagtattga
    34861 tttttttgat tagctgatta cgtaactctg taccaaatcc ttgtgaagct actgaagcat
    34921 agtattggca aatgatagca caaatcagac caatcacaga catagcgacc atccataagc
    34981 ccatttcaat gatttttcct cggtcattca tttttagtcc attatcgatc aaggaagcca
    35041 tcattaaagg caggacaagt tcgaagacag cttcaagaaa tttgaagaac gggcccaaaa
    35101 tgatttgctt acgatagtct tttgcataac gtaataactg aatcatatga ttcctcctat
    35161 tatttatttg tccacaattg aaaaaacaag tactaccaca ttgtagctta ggaaatttag
    35221 aatgaaaaga gaacaagcct tttcataaat gaaagtttta ttatgaagaa gctttagaaa
    35281 ttgaatagac tatttttttt aaaaaaaaga aaatgagaaa acatgccagt ctcttcattc
    35341 ataggaacgt ctattttact taatttttct ataaatgaat tctgcataaa aaatatatca
    35401 agtaaatgtt tatagatgaa actctattta cttctttttt aatttgttct tgcaatcttt
    35461 tttgctacgt aatttttgta aaaaagtggg ttgagagaaa tctaattgat tttcaaagaa
    35521 ggagtatgaa aatgagaaaa atatttattc gtttattgtg ggtactatct tagaggaata
    35581 aataatagtg gagaaaacta tggtttggcg cagattctat caggtttgat ggaacgaaga
    35641 caacctcccg acatcaagtc aaaagctact tgagattttg atctgaaaat aaaaaaagaa
    35701 aacaaggtag gaacatcaat gtttctacct tgttttcgta atcaatggga aaaaactaag
    35761 agtggtgtac gtaatcaata aaaaggatgg tgacaacagt acctctttac tctctgaaag
    35821 catcataact cgtttttcct cattatgcca ataaattgtt gttataccac atataaggat
    35881 ttgtaatagc taaaaacaga caggaaagtt gaaatctgtg aatcttattt tgtatggaag
    35941 gatttataga acgcaaaaat caagataaag atgaataaaa atcctgtttc ggctatatat
    36001 tgcgtatcta cgccaataaa acgagtttgt tctgtcatag ccatataaag caaaggcaaa
    36061 aatacaacta cagcgagata agatccgatg atttctcgaa aagttttcca ggaagtatgt
    36121 ttcctgtttt tatatttatc cgatttcatt gaatagtcat agcctcctta ttctttcata
    36181 tgttgaaatt ccagtataaa aactggaagg aactgtacgt tttttagaat aaaaattata
    36241 agggataagt aggctgctgt ctataataag atatattttt caattcttta ttaaagattt
    36301 tattgacaaa atgaatccgt tttctatgat ggaggagggg tgaataagtt gttgaagaag
    36361 gaaattttat gttacttaga gaaccagaca gcctttttcg atttagaaca tatgaacgag
    36421 gtctttacag cacaaaggtt agctgatcat tttggggtaa aaagaaacac agtcagtcat
    36481 tatttgaacc agttaaatga tgagcaacta ctagtcaaaa tcaattctcg accagtgatt
    36541 tatttccaca aagaagcttt tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt
    36601 tctatcgatg atctcaaaga ggaacagcct tttttcgcgc aaccaaaaga cttattctct
    36661 tttatgatcg gatacgacgc cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt
    36721 tactatccag atggcggatt accacttttg attacaggcg aaagtggcac aggtaaaagt
    36781 tatttagtta atttggtcta tcagtattgt ctgcttcatg atctgctaga agattctgca
    36841 ccattcatca cttttaattg tgcacaatat gcagataatc ctgaactttt gactagcaat
    36901 ttgtttggac atgttaaggg agcgtacact ggtgcagaag aaaataaaaa aggggctttt
    36961 gaagcagcag atggtgggat cttgttttta gacgaggtac atcgcttaac accagaaggt
    37021 caggaaaaac tgttcaccta tcttgatcaa ggtgtgattt atcgtatggg agaacctaat
    37081 ttgtcacgcc ggatcaaaac acgtctcttt tttgctacga ctgaagagat tactagtcat
    37141 ttcttaacaa ctttcattcg aagaatacct atccgaatcg aactacctgc acttaatcag
    37201 cgtagtcgta atgaacggct agaactgatt tactcttttt ttctcgaaga acaaagaaaa
    37261 atggggagcc ctttgtcagt cagtggacca gctttgtcac ttttaacaag tcagaaatta
    37321 tccggaaaca taggagagct gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct
    37381 gaacagcgtg aacagcctga aatccatgtc acgatccatc atttgcaaaa agaactactt
    37441 gcgcagccta ttactaaaaa tacagcacag caaggaatct atattacgca agaccagacc
    37501 ttagagcaat taatggagaa gcaacagccc gaacagcagc gaatcgttaa aagttttgag
    37561 caaatcctaa ttgatttcaa gaaaaataac tgtctgctat attcttgcga aggaaaatta
    37621 aagcaagaag tgattcaatt atttgatttt ttgctgtttg agaccagtag acaagaaaaa
    37681 cacgagctgc tggtttattg gacacaatct atccgagaga cctttcgaca aatggaaact
    37741 gcctatcaaa tcaaattcga tggaaacagt gtctatgcct tgagttacta tctctatcag
    37801 cgaagcacta tcaaatggtt accggaagaa catgatatta tccaactaat caaagaatta
    37861 gaacaacaag tttctcagtc ttatccatct agttatcact atgtccaacg aattctggaa
    37921 ttaagtaaga tcaaattaga tattgaagac acaagtatgg atcggatttt actgacgatc
    37981 tatctaaaaa aagcgaaatg gtcaaaagaa aaacgactgc caaaagcgat gattgctgct
    38041 cacggttatg caactgctag cagtatggca aatgtagtaa atcgtttatt acaagaggat
    38101 ctatttgaat catttgatat gccactagat gtcacaccac agcagattgc ttcagaaatc
    38161 cttgattatt gcgaaaacag cgatgtttca aatggattga tcatattagt agacatggga
    38221 tctctgaaag aaatccatca gttttttaaa aaacagttat ccgtaccgct tttgattttg
    38281 aataatgtga cgacaccttt agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca
    38341 ttagaagaga tagcagaaga gactgttcga cagatccagc cagaatggcg actgctctat
    38401 ccagaggaaa acaaacccaa agcactcatt actacttgct tcactggaat cggtacggcg
    38461 gtccacttaa gtgaactgct agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc
    38521 ccatatgaat atcagcaatt aaaagataag aaaaacagtg acccattatt ttccatatac
    38581 gaagtcgtag gaatgattgg caccacagat cctgatatca ccaatatcga ctatctttca
    38641 ttagaagacc tcatctcggg ggaaaagatg tctgtactag ctgaatggtt ggacactact
    38701 atgaatgctt cagagaagga aatattcaat cagcgcatca ttcgtaattt ttcattggaa
    38761 aaagtactgg attccgtgac tattctagat acagagaatg tgatgggaga aatcgatcat
    38821 tttatgagag aattagaggt gcagttgaat cgaagcatca gcaatcccaa aaaattagcg
    38881 ttgtatgtcc atgtgagctg tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc
    38941 tatcaaggat tagagaaatg gaagcagtgt caaaaagaag ggttatctgc tattaaatct
    39001 gcgtttagtg tcattgaaga gcattatagt gtcatgatcc ccaattctga gattgcctat
    39061 atctatgata ttttgagtga agatacagag tttctctcga tagaagaaga attttgaaca
    39121 taagcaagtt gttgccacta
//
LOCUS       DACDTO010000017        37229 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDTO010000017 DACDTO010000000
VERSION     DACDTO010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.017"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            74..823
                     /locus_tag="IWK70_000934"
     CDS             74..823
                     /locus_tag="IWK70_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9613500.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1247..1417)
                     /locus_tag="IWK70_000935"
     CDS             complement(1247..1417)
                     /locus_tag="IWK70_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9613501.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1611..1790)
                     /locus_tag="IWK70_000936"
     CDS             complement(1611..1790)
                     /locus_tag="IWK70_000936"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613502.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2177..3289
                     /locus_tag="IWK70_000937"
     CDS             2177..3289
                     /locus_tag="IWK70_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9613503.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3367..3660)
                     /locus_tag="IWK70_000938"
     CDS             complement(3367..3660)
                     /locus_tag="IWK70_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9613504.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3828..4142
                     /locus_tag="IWK70_000939"
     CDS             3828..4142
                     /locus_tag="IWK70_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9613505.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4163..6142
                     /locus_tag="IWK70_000940"
     CDS             4163..6142
                     /locus_tag="IWK70_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9613506.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6155..6481
                     /locus_tag="IWK70_000941"
     CDS             6155..6481
                     /locus_tag="IWK70_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9613507.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6497..7015
                     /locus_tag="IWK70_000942"
     CDS             6497..7015
                     /locus_tag="IWK70_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613508.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7015..8367
                     /gene="celB"
                     /locus_tag="IWK70_000943"
     CDS             7015..8367
                     /gene="celB"
                     /locus_tag="IWK70_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9613509.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8451..9956)
                     /locus_tag="IWK70_000944"
     CDS             complement(8451..9956)
                     /locus_tag="IWK70_000944"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9613510.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10048..10638)
                     /locus_tag="IWK70_000945"
     CDS             complement(10048..10638)
                     /locus_tag="IWK70_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9613511.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10817..12463
                     /locus_tag="IWK70_000946"
     CDS             10817..12463
                     /locus_tag="IWK70_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9613512.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12512..13231
                     /locus_tag="IWK70_000947"
     CDS             12512..13231
                     /locus_tag="IWK70_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9613513.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13710..14657
                     /locus_tag="IWK70_000948"
     CDS             13710..14657
                     /locus_tag="IWK70_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9613514.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14669..15589
                     /locus_tag="IWK70_000949"
     CDS             14669..15589
                     /locus_tag="IWK70_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9613515.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15630..17087
                     /locus_tag="IWK70_000950"
     CDS             15630..17087
                     /locus_tag="IWK70_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9613516.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17432..18046
                     /locus_tag="IWK70_000951"
     CDS             17432..18046
                     /locus_tag="IWK70_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9613517.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18039..19763
                     /locus_tag="IWK70_000952"
     CDS             18039..19763
                     /locus_tag="IWK70_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9613518.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19760..21211
                     /locus_tag="IWK70_000953"
     CDS             19760..21211
                     /locus_tag="IWK70_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9613519.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21684..23837)
                     /locus_tag="IWK70_000954"
     CDS             complement(21684..23837)
                     /locus_tag="IWK70_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9613520.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23839..24885)
                     /locus_tag="IWK70_000955"
     CDS             complement(23839..24885)
                     /locus_tag="IWK70_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9613521.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25035..26324
                     /locus_tag="IWK70_000956"
     CDS             25035..26324
                     /locus_tag="IWK70_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9613522.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26337..29039
                     /locus_tag="IWK70_000957"
     CDS             26337..29039
                     /locus_tag="IWK70_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9613523.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29024..29881
                     /locus_tag="IWK70_000958"
     CDS             29024..29881
                     /locus_tag="IWK70_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9613524.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29952..30809)
                     /locus_tag="IWK70_000959"
     CDS             complement(29952..30809)
                     /locus_tag="IWK70_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9613525.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            31020..33191
                     /locus_tag="IWK70_000960"
     CDS             31020..33191
                     /locus_tag="IWK70_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9613526.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33259..33600
                     /locus_tag="IWK70_000961"
     CDS             33259..33600
                     /locus_tag="IWK70_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9613527.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33876..35630)
                     /locus_tag="IWK70_000962"
     CDS             complement(33876..35630)
                     /locus_tag="IWK70_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613528.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35641..36477)
                     /locus_tag="IWK70_000963"
     CDS             complement(35641..36477)
                     /locus_tag="IWK70_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9613529.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36479..37162)
                     /locus_tag="IWK70_000964"
     CDS             complement(36479..37162)
                     /locus_tag="IWK70_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9613530.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12011 a         6548 c         7635 g        11035 t
ORIGIN      
        1 aactcaagcg ccaacggggt atgaattaga tgcgacaccg atcgagtttg agatcgaaag
       61 aagtcagaca gcggtggtag aattgacgaa agaaaaccga cttacaccag gcggtgtcgt
      121 gttgacaaag atcgatgacc aaagcgggga agtcctgcaa ggagctgttt ttgagttaca
      181 agatgccaat ggaaccgttc tgcagtcagg tctaaccaca ggagcagatg gaaaactggc
      241 gattgacggc ttagcaccag gagtgtatca gctggtagaa acccaagcgc caacggggta
      301 tgaattggat gcgacaccaa tcgagtttga gatcgaaaga agtcagacag cggtggtaga
      361 attgacgaaa gaaaatcgac ttacaccagg cggtgtcgtg ttgacaaaga tcgatgacca
      421 aagcggagaa gtcctgcaag gagctgtttt tgagttacaa gatgccaatg gaaccgttct
      481 gcagtcaggt ctaaccacag gagcagatgg aaaactggcg atagatggct tagcaccagg
      541 agcatatcag ctggtagaaa ctcaagcgcc aacggggtat gaattggatg cgacaccagt
      601 aactttcaag atcgaaaaag aacaagaagt ggcgatcttt ttaacaaaag agaatagaaa
      661 agttgctgat agctcagata taagaggaaa caatccaaca agcagtggaa ataagcatct
      721 tccaaaaact ggagaaacca tcattagcca gtttatattg tcaatattgg gtgtgctact
      781 tgttttcatc agtataaaat ttagaaagaa acgaaatata tgagctaaaa taaaaaattg
      841 aataagcata gtttttaaaa tcaactagta cccatcaatt gaatggaaac taccgatttt
      901 agaaggggta tacaaacaaa tattagtatc ggagtagaga aggttgaacc caaccagtaa
      961 ataagtaaag acaattttgt gctgcgttga gaagtgaaaa ataaaccttt gtagtaatac
     1021 tagttaataa aaaatataca aaaacactgc gaaaatcatt cttgagtttc gcagtgtttt
     1081 ttgcctaatg actcaagttt attgggtaat actttgattt tctttctaca aataatcagc
     1141 gatcagttgt aattttttct ctagttttaa tggctctttg tcaataagga gaaaaacaag
     1201 tcgggtcttt tgtctcggaa agcttcaacc aactcgtgaa taaaggttac gcctccttga
     1261 aaggcgtgga aaaaccaagt aatcgatcaa tcaccaattt cgcatttggt agaacatgtt
     1321 tggtgagctg aaagtaggtg gcgttcatgt ctgtaactaa gaattctatt tctttaggat
     1381 tagtgcagct aaggaaatag tttgttaatc gaggaatttt ttgtgtaggt aaattttctc
     1441 gattaatttt tctgtctcgc catccgcgga aataaaactc agtttatcct caacggaaac
     1501 gtgagaacga aattcacaac cctcaacact caagacagaa ttcttttgga agactttggt
     1561 aaatagcttc tcaactcttt caatgtgcga ataacggttg tcaaagatac ctaacaactt
     1621 ttcgaaataa aaaataacga cactttttca gtcagtaaag aagtgatttt atgtctgaca
     1681 tgatttgaaa tcgagtgcct tggacggata aaataacttt gagcgttcca atggcttcga
     1741 cagtttttac aggtatagcg ctgctttttt aggcgtataa ccattggcat atgattatat
     1801 tattcaaagt ggacaatggt ttccttcttc ttgtttttca ccaccattaa tttcctattt
     1861 ccatctacca cagcagaacc acaatttcta cagacacatg gagcagggaa gagaactgca
     1921 gcaacgatca atgttttttt cttctgtaag gtttcgtaag agacctctgt aatcatcaaa
     1981 tttttgtcta ttgatctcag aatttttttg atagaaccat tcataaagca tatcgttctc
     2041 tcagttgttt attttgtggt gatttaatca tacaagaaaa cgatatgttt ttttataaaa
     2101 acatgaatag gaaaaatagt tgacaagaaa atgacaacgc catatgatgt tccatagtat
     2161 ttacaacctg tcatagatga ctgtaaaaaa taaaagtaaa aagaaaggac gacaacaggt
     2221 ggatttttat cagtctctac aactagatcc gtttatttta aaacaaaaaa tacgagaagc
     2281 agcttctaaa aaagaaaagt gcatgtatat ttgttcattg tttttacgat cattattcat
     2341 cgtattgttt gccatttgtt ttatcattat aattacaaca ttatttgaat cgaagcataa
     2401 accatacgct gtggttttat tctgtatgtt gatgagtatt cgttttgttg actttggtta
     2461 taagatttcc cattcgatca taggtttagc aatcgtcatg ttctcactat tagtagcacc
     2521 gtatgtccag ctgatcaaat ggtcagttat gggcatgttg attcatttta ttttactaag
     2581 cagcatatta atggcaaccg ctagtgatcc caaaatgggg aatgcttctt tgtacggctt
     2641 ttcatatctc tttattgttt attcgttacc taaagattcg ttgaacaaga atttctttac
     2701 acaaacaagt agcttattat ttttgttttt ttgttggttt tcagttctcc tttacagaaa
     2761 acatcgagaa aagaacaaag acaaaagctt gtttaaagag atttttttga aaggcatgta
     2821 ttcgcaggaa aaaatttgga tgctcatcta tgcatttggc attagtcttt tgatcgttgc
     2881 aggagagtat gtcccttttc agcgattgat gtgggcaggg tttgcatttt cttctattgt
     2941 atctagctat ggattgatgt cgattggttt caaagaacga gcagtagacc gaatcattgg
     3001 ctcattgatt ggatgtgttt tatttattgg tatcagccaa tttattcctt ttaactgggt
     3061 cggtatatta ggtggtctag ctttaggtgt ttgttctacc tatagatata aaacagtttt
     3121 taattctttt ggtgctttgg caatcactgc cagtttattc ggggttccag gagcagtaac
     3181 gatccgcatt tttgagaata tccttggggt atgtttagga atcatgtata ttggggtgac
     3241 tgaaatactt atccgaaaaa taagagaaaa acacggtttg aatcattaga tgagatcgaa
     3301 taaaaaataa ggaaaaagtg aagatacaca tggatgataa attgctatca ttccatgtgt
     3361 atctttttac ggtaaatcag tgaaacgttc gactttcatt tttaatttat actttttacg
     3421 gagttcgttt atttgagcca tcatttcgga tttgtgatgc ttttcgattg cttcttcatc
     3481 tttccaacgg tcgatcagta aaagagattc tggatcttct agtggaaagt aatagtcata
     3541 tttctcgttt ccttcttctg cacgaatcgc tttgacaatg tttctttcga ccatttcttc
     3601 tgcaaatttt ctggcttgtc catttgtact tgtatacaaa atattcactg tgatactcac
     3661 taaatcccct cctgaaaaaa ttctatcact agtatatcac ggtgccagaa attattattc
     3721 aagatatgat acacattttt tccgcaatgc tacggaaata tatattttga atagaaaacg
     3781 cttacaagat atgcttggtt tataaaaata aaaggagtga ttgatttatg aaaaaagcat
     3841 tgattatttg tgcggcagga atgtcatcct cgatgatggc ctcaaagaca acggatttct
     3901 ttaagcaaaa aagagaagag gtactcgtag atgcagtttc agctacagaa ggcgataata
     3961 tgattaagaa tagtgatttt gatttatttc tgatcagccc gcaaacgacg atgtttttgg
     4021 ataaattcaa aaaattagga gaatctgttg gaaagcctgt tgttagtatc cctttccaag
     4081 cgtatgtccc aatccctaca ggcatccaga agttagcaga ggtgatcgaa gagaacatat
     4141 gatggaatag gagtggatga taatgaaaca gaaggaaaaa ttgctgttga gatatttgat
     4201 tgatcacaag aaggagtatg tcaccagtca acgtttagct tctgaattgt tattatctga
     4261 tcggacaatc cgtaattact tgcagcggat caaagagtta gtagaaaaaa acggaggtaa
     4321 gatcattgct aaaccaggtt atggctacca actgcatatt ttacaaagac tgacatttga
     4381 cttgtttttg agtcggcaag aaatagtacc tggatatgct agagaaccgc aaggatttta
     4441 tgagtccgaa gatcgacaaa agtatatatt gaaccaattg cttttggaag atgccattct
     4501 ttttatggat gatttggcag aagagcttta tatcagtcgt tccagtttga caaaggacat
     4561 gcaagaaatc aaggagcgat tgattccata ttcactaaaa atcgtttcta agcatggtca
     4621 agggacatgg atcgaagggg aagaaagaaa tcgacggcat tttatcatgg atactttttt
     4681 tggaaaccat tatggaaatt cattgaaaga atatttaggg caaagccgtt ttttcccaga
     4741 catcagtttt gaagagctag tcattatcat tctcgatgaa acaagagaag ctaagttaaa
     4801 agtttcggat tttatcatac agaatctgac tttacacttg gctttgggga tcaaacggct
     4861 ccgagaaggg tttgaaatca aggaattagg catcgaacaa gaagtttcta agcgagtaga
     4921 gtatcaagtg gcgcagcgga tcgttcaacg tattgaggta atcgcaggtg tatcgtttcc
     4981 cgaagaagaa attgcttatt tgacattaca tctcatggct aagtcgaatc acaaagaaaa
     5041 tcaaaatgat caggaactat caaaggaatt agcgaaagtt ctgaaaagat tgtctcagga
     5101 atttcatttt tcattagtag aggatctcca gcttttcaat ggactactcg atcatttgtc
     5161 accgatgatg attcgattga gccgaggtat tcgcattgag aacccattga ctgaggagat
     5221 aaagaaagaa aatcccaaag tctttgacgc agtaaaaagg catttcagca acatgccggc
     5281 tttgaaaaac tataccatca atgaagatga atgggcctat ttggctttgc atctcatggc
     5341 ggcattggaa aaagaaagag ctgcgcataa actacatgca ttgattatct gtgcaacggg
     5401 ttacggcagt gcccaactat taaagaatcg cgtagtcagt gaattcggta aaaatattac
     5461 agtggtaagt gtaaaaggct attatgagat caatgaaaca actttaaaag gaatcgacct
     5521 gattatttct tctattgatc tttcgacaat gtttttcaaa attccagttt tacatgtcag
     5581 tatttttttg aatgaagatg atgttcgtaa aatacgaaag gtagttggag aatctatacc
     5641 ctatcattcc tcagatagac cgataccagc attttctcta cagcaaaaaa agcagattta
     5701 caccgaacag ttgtctgaaa aatttttcaa gacttatacg tacgaaccga aaaaggaaga
     5761 aattcttcat gatttgctgc aaaagctgag cataaatgaa gaagaaggat atataagaga
     5821 aatgcgaaag cagatccagc aacgtgaaaa tatgggacaa atcatattca gtgatacagt
     5881 ggcagtccca catcccgctg ttccagttgg aatcgaaaca aaggtagccg ttgcattgat
     5941 tccttctggg atgcagtggg atcaatatca agaaattcat tttgtttttt tagtttcgcc
     6001 ttcttatgtc gaaaatgaag gaattacggt cgtgactaaa gcaatcgtta ggttagttga
     6061 ccagctaaaa gtacagcagg aaatcttagc tgaacctatt tttgagaatt tcagcagtaa
     6121 atttatcaaa ctgatcgaat aaggaggaaa acagatggct gaaaaaataa acagtgaaac
     6181 actacaagtg acttcttttg atatcatttt ccatagcgga aatgcacgga cactgataca
     6241 tgaagcattt cgctcgatgc gtaaagagat gtttgaagaa gtagaagaga agttgcgaca
     6301 agcaaatgaa gagattcttc atgcgcatca gtcacaaact caacttctga aagaatatgc
     6361 cagcgggaca aaaatagaaa tggaaattat catggtccat gcacaagatc atctgatgac
     6421 gacgatgaca ttattggaag ttgcacagga aatgaattat ttgtatcaaa aaaataaata
     6481 gaaaggaagg atcattgtgc aggatacagt aaaaaggaaa atcgaattag aaaaaaagca
     6541 acttagtttg aaaaacatgt atttcaatcg ttatctaatc gtacgttacc tgactgcctt
     6601 tttctttttc atgaacatgc aatggatgat tctgcttcta tcagctaaaa gtttaggaag
     6661 tagtttaccg atgattctga ttttagctat tttgcctgca gtaggagaac aagtaaagct
     6721 ttatcgaaaa caccaaacga acgttccatg gacaaagtgg tattttttgt tccagagtgt
     6781 ctttaattta ttagtgattc cagtgttgtt tacctcagga tttacattct tgtatccttt
     6841 tatggcaaat aacaatggag gacagttatt tgttttcatt cttgttgtaa gcggtatttt
     6901 tgccagtgca tttatacagt atcgtttaaa gaaaatttca ctcaaccaag accaacagta
     6961 tatccgtatt aaacaatatg aaaaagcgtt atatttagga aaggagaata actaatgttt
     7021 gattttttgc agaaatacct gatgggacct atggggaaaa ttgcgcaact taaaatcgtt
     7081 cgggctgtca tggcagcggg gatggcttct attcctttca cgattgtagg ctcgatgttt
     7141 ttggttttaa atattttgcc attaccgttc ccatttttag aaggattttt taatgcaact
     7201 tttttcaaag ttagtgactt gtacatgatc gtcaatacaa tgacgatggg gattttatct
     7261 atttactttg ccattgtgtt tgcttatgaa ttaacgtcaa ttgaacgaga tgaacaaaaa
     7321 ttaaatgtta atccgctcac gggggcatta ctatctgttt ttgcattctt tatgtgtatc
     7381 cctgaattga tcatgactga tggtaaaatc agcttgattg cttctatcaa tgagacagaa
     7441 actgtagtaa atggtattag gatgggctct tttgtcgaac gtttaggaac ttcaggaatc
     7501 tttacagcta ttattatgtc gattattgcg gtcgaattgt attgtatgtg tgtcaagaga
     7561 aattgggtca taaaaatgcc agatgttgtt ccgccaggag tttctcgttc tttcactgct
     7621 ctgatcccaa catttgtcat tgcgtttgtg gtgatgatca tcaatggatt attagttgca
     7681 ctaggaacgg atatcttcaa aatgattgct atcccatttg ggtttgttac gcagttgacg
     7741 aatacatggc ttggtgtcat ggtgatttat ttcttgatcc atgcattgtg gatcgtaggg
     7801 atccatggag cgaatatcat cacttctttc ttgaccccga tcgtattagc taatatggca
     7861 gctaacgcac aaggcgcaaa ttatccgttg gcaggagaat tcaacaattc ttatgtaacc
     7921 gttggtggat caggtgcgac tttaggattg atcatcttta ttgcattcct cgcgaaatct
     7981 gatcagctga aagtgttagg aaaagcctca cttgtaccag gattattcaa tatcaatgaa
     8041 ccaatcattt tcgggatgcc aatcgtctac aatccctatt tagcggttcc gtttttctta
     8101 gctccgatgg cttctgctac actcgcttat tttgccattc ggctggaaat cgtcaaaccg
     8161 atgcttgcac agatgccgtg gccttcacca gtcgggatcg gagcatttgt tggtagtggt
     8221 ggtgattgga aagctgctgt gttagcagtc atttgtgcaa tcatcgcatt cattatctgg
     8281 ttcccattta tcaaattcta tgataataaa ttattaaatg aagaaagaga aaaggcagca
     8341 gaattagcta gtgaaaagtt aaaatgattt ctaagaaaaa aggaactgtg acaaaaaatc
     8401 acagtccctt tttttcttag aaatgggaaa ccactagtat ataaatacca ttattcttca
     8461 atcaaccgct tcacgattgc ataatcccct tcataaggtt catctacacc attgattttt
     8521 tgatagggat ccacgccttt tcccgcaata acgactgaat cttgcggagc gctttctgct
     8581 aaagcgcgtc gaatcgcttc aggacgatcc atttcataat gaacgatcaa atctggatta
     8641 gtaattgctt cattgatttc ttccgcgatt tttttcggat cttcaaatgc tgggtcatct
     8701 gccgtcaaaa aggcgatgtc agctgtttct gatagcactt ttccaaaatc atgtcgacgg
     8761 gagattgctt tgtttcctgg gctgcctaat acaacaatta cacggccatc tggatgttca
     8821 tttttggcaa atttcagcaa tgttttcaag cttagataat tatgtgcata gtccacatag
     8881 acatgcgctc cattggattg gatcaattga tccattcggc ctggtacacg agcttctttt
     8941 aatccgtctt gtgcatcttg actggaggca cctgttagtg ctgcggcaat gatcgcactg
     9001 gctgcatttt cttggttgaa tcctccagcc agcaaaatat cataagcagt atcgcagata
     9061 gctaagcgat cttcatgaga agataaagta aaaccgtgag tacctttatc tgaacggatc
     9121 acttgataat tgctttcttc cgaacgacca taagtgatcg ttggaatctg gaatagatcg
     9181 gctgtttccc taagtaaatc aaaatagtca ctttcatgat ttaaaacaac tgtttttgaa
     9241 ttcaagatca attgtcgctt gcaatagaaa tagtcatcaa atgtcggatg ttcgatcgga
     9301 ctgatatggt caggcgagat attcaagaag attccgacat ctaaagtcaa gccgtatacg
     9361 cgttgtgttt tgtaagcttg tgaagaaact tccatcacta gatgagtcat accattttca
     9421 actgcttctg acatcatacg atataaatct aatgactcag gcgttgtcaa atgggatttg
     9481 aaatatgttt tgccatctaa cgttgtattc atagtagaaa gcaatgctgt ttttttattt
     9541 gttgtgtgat ccaagattgc ttttgtaaag tacgcagcag ttgttttacc ttttgttcca
     9601 gtgaatccga tcactttcaa tttattttgc ggatagtcgt aaaaagccat agcaagaagc
     9661 gccattgctt tacggatatc cgttacgatg attcctactg ctgaattttc gtatggttgt
     9721 tctgatacat aatattgaat accctgttcg attgctgaat ccaaatattc ttctttgaaa
     9781 ttcattcctt tacagaaaaa caaagtagaa gcatcagctt ttcttgaatc ataacttaac
     9841 tgaagaaatt cagctttttc tggtacatca agatgccagc ctcttgttga aacaaattct
     9901 tttaatagat tttcttttaa tagtaaatga cggatgtctt ctaaagagaa gctcattgat
     9961 tgatccctcg ctttattcaa tttcttggtt tattatagca cactcttttt aatatatcgt
    10021 ttgtttccgc tatgacaaaa tccagcttta ttcaaatttt cttgacacag gggacaagcg
    10081 gatgagtatt ttcataaaca atgaactgac tgtaatagat aggagaacga atagtgtctc
    10141 caacaatcca aaaacataat aattcaaaaa gacgataaga ctattggtaa gtaaaagtcc
    10201 ttgcggcatt ggaagccgaa aaaatttgtg gaaaataatc ggcggtatcg ttataccgcc
    10261 atttgaggcc ccgactgtgt acaatgtgta aaccccaaga ctgaacaaaa gacttcctga
    10321 aatcagtgaa aagaaatgtg aggagatcat ttccactttt ggaacaatct ctaaaacaac
    10381 aggaaaaagc agacttcccg ctacagtctt aaaaaatact gattttccta ataggacagc
    10441 ggctagaagc agcatcataa gattgatgct ccaaacagta actgagcgtt ggatagaaaa
    10501 ggccgtttca actaaatgtc cgatggcgcc cactccggcg gaagctatat cgtgagggag
    10561 caagaaccaa ttcaatccaa ctgtgataaa gaaaagacct aaaaatataa cgggtagttt
    10621 ttttatttct tgtgtcattg atatctccta taaatgttag ccataaaagg ctgtatattt
    10681 ctttacttta ccaaaaaaac gaaaccgaat tcaaaaaaac aattgttctt ataaaagtta
    10741 tataaaatcg attgccagtt tctgttttac caattaaaat gatagtaatg ttttgtagaa
    10801 agttggaatc tgaccgatgg ataatcaaag aaaaccagca ttagatcaat taacaccgca
    10861 agaaaaaatg gcacgaggct ctgcttggat gaccgccagt aatatcattt caagattgct
    10921 aggcgctgtc tatatcattc cctggtatgc ttggatgggc gaaaatgcta aggctgccaa
    10981 tggattattc aatatgggat acaatatata tgcattgttt ctactgatct ctacagcagg
    11041 aatacctgct gcgattgcaa aacaaactgc acgctataat tcattgaatg aatatggcac
    11101 ttcacgacga ttatttatcc gtgcattaca gatgatgggc gggttaggcc tgttgtttgc
    11161 tttgttcatg tacatcgcct ctccttggct agctcatgct tctggtggcg gagaagaact
    11221 gattccaacg atgcgttctt tgagtatcgc tgtattggtt tttccttgta tgagtgtgat
    11281 acgagggtat ttgcaaggca atcaagaaat gatgccttat gcgctgtcgc agattgtaga
    11341 acaggtggca cgtatttttt atatgctatt ggctacattc atcatcatga aagtcatgga
    11401 tggtgactat gtgacggcgg tcacgcaatc aactttcgcg gcatttatcg gtatgctagc
    11461 aagtattctt gtccttttgt attttttgaa gaaacatcaa gcgtatacat cggcgtttat
    11521 tcactacagc gaaaacaaaa taaatattgc aacgaaggaa ttgttgttag ataccgttaa
    11581 agaagcgatt ccgtttatta ttgtgggttc aggggtaacg atcttcaaat tagtcgatca
    11641 gttcactttt atgaagatca tgtctgcatc caccgagtat tccaatgcac agttactgga
    11701 tcttttttcg atttttagtg ccaatccaga taagttgacg atggtagtta ttgcattggc
    11761 aacatcgatc gcgtctactg ggttaccgtt gattacagaa gctgttacgt tgaaagatcg
    11821 agtaggtctg gcaaaattga caagcggaaa tttacagttg ttttcatttt ttatgtttcc
    11881 ggcgacattt ggtgttatgt tacttgccta tccgctaaat acattatttt atacaccgga
    11941 ttctctagga agtaatgtac tgatccaagc aagttttgta ggactcttcc taggactata
    12001 tatgctagtt tccaacatgc tgcaaggaat gtttgaaaac aaggcggcaa ttaattattt
    12061 ggttattggg tttctagtga aactccttct tcaataccca gccattcgtt tatttgaagt
    12121 atacggccct ttattggcga caatgatcgg atttgctgtg tcttgtacct tgatcttaaa
    12181 gagaatccat gaagtagccc gctttaaacc aagttttgtc tggcgcagga cattgctgat
    12241 ctttattctt accttgatta tgcttttggc ttcgtggatc acaaagatga tttttggtgc
    12301 ttttttaaac gaagatagca aattccagtc tctattgttg attcttgtag tagcaggtat
    12361 aggggcttta gtgtatggtt atttagcttt gaaaatccgc ttggcagaaa gactactagg
    12421 accaggaata gcgcgtttac gacataaatt aaaaataaaa taagtgaatc agaagagcta
    12481 gattgatgct cttttgaaat gggggaaacg catgcgttta gataagtttt tagcagatat
    12541 gggacttggc agccgcaaag aagtcaaagg attaatcaaa aaaggaacga tcgaagtcaa
    12601 tggacaagtg gtgaaaagcg ataaagcgca agtaaatgaa ttcgaagatg cggttactta
    12661 tttaggagaa atcattacgt atcaaaaaga tttttactac attttgcata aacctgcagg
    12721 agtcatttct gctactcagg ataatcatga tcaaaccgta atcgatttat tggaagacca
    12781 ggattatcgg gaagatcttt ttccagtcgg ccgattagat aaagacacag agggcctctt
    12841 gattttaacg aatgacgggg tgtttgccca tcagttacta tcacctaaaa aacatgtgga
    12901 gaaagaatat ctagctgagg ttagcggtat aatgacagaa gaagatatcc gtttatttgc
    12961 agaaggactg attattgatg gcgatgagca gacattgccg gcaaaactcc aaattgatca
    13021 agtagatcaa gagacagaaa cttcaattat ccgtctgatt ttgcatgaag gaaagtttca
    13081 ccaagttaag cgaatggtca aagcagtagg aaaagaagtc ttatatttaa aaagaatccg
    13141 tatgggaaaa ttttggctgc ctaaagattt gaataaaggg caatatcgac ccatgaccaa
    13201 agaagagcta gaacaagtga gagaaaaata attgaatgga tagctaaaag aataagcaga
    13261 gtagttgtag cgccatctga aaagcagact tgtccgtctg actttcggat ggcttttttg
    13321 tgaaatttcg gcttatttcc gtagaagcta cttctggaac accgtttgtt cggaataagg
    13381 gactgtgaca agacttttgt tacagtcccc attgttcctt gtgactcaga gttagatagt
    13441 aaggacacga tctccctaat actcaggcca aaacgctgaa ttcacaacct ctgataaacg
    13501 gtgttcaatc agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt
    13561 ttcccgtttt tttctctaat actcaggcca aaacgctgat ttcacaacct ctcctatact
    13621 ttgtttcatt ttccctatac tttgtttatt tattctcatt attgaaagcg ctataattac
    13681 gttgcgaatg aatcgaaagg ggtttttaga tgaaaaaacg aaaggggttc aatcattttt
    13741 ttatgaatct atggcgttat aaagcacttg tcctgatggc tgtaccgggt atgctttgga
    13801 tgattttttt cttttacata cctgtaatgg cgaatgtcgt agcattcaaa aattttcata
    13861 tttcagcaga tgggttcttg gctagcttga gggaaagtcc ttgggtgggt tgggaaaatt
    13921 tcaaattttt attttcttcg gatcaagcat ttctcatcac taaaaataca attttatata
    13981 atgtagcatt tatccttttg aatctgctga tctcagtcgt atttgcaatc attatgagcg
    14041 agttgagaaa taaacgactc gttaaagttt atcaaacgat gtccttacta ccttattttc
    14101 tatcttgggt cattatcagt tactttgttt acgctttttt aagtcctgac aaaggaatat
    14161 tcaatcagtg gatcactgga catggaggag aagctgtcaa ttggtataat gaaccgaaat
    14221 attggccgtt tatcttggtg ttcattggta catggaaagg gattggttat aacagcatta
    14281 tttactttgc atctgttatg ggaatcgatc cgacttatta tgaagctgct atggttgatg
    14341 gagctagcaa atggcagcag atcaaacatg tgacgatacc acaacttatt ccactaatga
    14401 cgattttaac gattttagcc gtaggaaaca ttttccgagc tgattttggc ctgttttata
    14461 acattcctag gaattccggt gcattatatg atgtgactca agttttggat acgtatatct
    14521 acaatggcct gacatccaca ggagattttg gtatgacggc tgctgcagga ctttaccaat
    14581 cagttgtcgg ctttgtcttg ttgatgatca caaatacgat cgcccgacgt tttgatagcg
    14641 attctgcatt attctaagga ggaaaaaaat gaaaaaaaag aaagtggaga gagtccaagt
    14701 ccgctctttc aataagacgg ctaatttttt ctctaatata atgattgcac tctttgctat
    14761 ttcctgtatt ttgccattta tatttgttat cgccatctct tttaccgatg agtccatatt
    14821 gtcgactcac gggtacagtt tttggccaga aaagttcagt atatttggtt atacgtactt
    14881 gttcgaccag atgcaggata aaattttcca agcgcttttc gtcactgtac ttgttactgt
    14941 tttaggtaca ctgatcaata gcacggctac ttctttatat gcttatgcga tttcacggtc
    15001 aaactttcca ttccgtcgtt tttttaccgt tttttgtttg attacgatgt tgttctcacc
    15061 agggatggtg gccaattatc ttgttatgac aaatcttctt cagttaaaag atacgatttg
    15121 ggcattgatc ttgccaatgg ctgtcagtcc cttcaatatt attgtgatgc gcacattctt
    15181 taaacgttcg gtctctgatt cgattattga atcggcacgg atcgatggag ctagcgaact
    15241 gcgaatattt gttcagatcg ttttaccgtt agctgttcca ggtattgcga caatcagttt
    15301 atttgccgca ttgggttact ggaatgactg gttcaatgct ttactataca tccaagatga
    15361 taagctagtt ccactacagt atcttttgat gaaaatccaa agtaatattc aatatctgac
    15421 acaaaattca ggagcaggaa gccaattgac aggcggatta gcctcagttc caggagaatc
    15481 tgccagaatg gcgatcgtag tcatctcgac gttgcctatt gcgatcagct atccattttt
    15541 ccagaagcat tttgtcaaag gtctaacaat cggcggagta aaagaataaa gaaacaaaaa
    15601 tataaatcat aaaattagga ggaagaaaaa tgaagttatg gaagaaacta gcctttacag
    15661 gtgtttcagc gttgttgttg ggaacattag cagcatgcgg aggttctggt tctaaagacc
    15721 aggcagaagc tagcaattcg gacactttgc agatgtatca aatcggagat aaaccagata
    15781 atttcgatca attgatggaa gtagcaaaca aacgaatcga aaaagagatc ggtgtaaaag
    15841 tcaatatcaa ttacattggc tggggcgatt acgaaaagaa aatgaacgtc atcatttctt
    15901 ccggggaaaa ttatgatatc gcctttgcga acaactatgt ttccaatgcc caaaaaggag
    15961 cctttgctga tttgactgaa ttagcgccta agtacgctga aaaagcttat aatgatttag
    16021 atgaagcata tattaaagga aatctggtca atggaaaatt atatgctttc ccagtcaatg
    16081 gaaatgtctt tgctcaacaa gtacttactt ttaacaaagc tttactagat aaatacgatc
    16141 tttcaattga tggaatccaa tcttacgctg atgcagaaaa agtcttgaag gaattccatg
    16201 aaaaagaacc aaacacggca gcttttgcca ttggacaagg cttcaaagtc caagggaact
    16261 ttgattatcc tttagggaat acgttaccat ttgctattga tctggatgga gatgattcga
    16321 aaatcatcaa ccaatacgat aatgaaacgt atatcggact gttacgtaca atgcatagct
    16381 ggtacaaaca aggactcatt ccaagtgacg ctgcaacgag caataaggat tatccactag
    16441 aaggaaatac ttggctaatg cgtggagaaa cacaaggacc gtatgattat ggcgatacaa
    16501 ttttgacaaa tgcagcaaaa caagaacttg tctcaaaagc aattactgtt ccactaaaat
    16561 ctacagctca agcacaaatg gcaaattttg tcgtatcaaa tacttcaaaa aataaagaaa
    16621 aatcagtaga acttctaggg ctgcttaaca gtgatccaga attattgaat gggcttgttt
    16681 gggggatcga gggagaagca tgggaaaaag tcgatggttc agatgataaa atcaaattgc
    16741 tagatggtta tcagccaaat acacatatgt ctgcttggaa tacaggaaac aacaagattc
    16801 tatatacaca agagtctatt acggatgaca tgattgccaa acgtgatcaa tcgattgctg
    16861 atgcagaaac ttcaccgatt ttaggattca gcttcaacac tgacagtgtc aaaacagaat
    16921 taagtaatat ttctaatgtc atgaatcaat atttagatgg attgaataca gggactgttg
    16981 atccagatga aacattaccg aaattgaaag acgctttgga caaggctggc tatgataagg
    17041 tattgaaaga aatgcaaaaa caatatgatg agttccgaca agaataataa tgaataaaga
    17101 caaaggggtt gtgacaaaag acgtgtcaca acctcttgtt ccgaataaac ggtgttcaaa
    17161 agccagtttc tcggtattaa gctaaatata tgtaaaaatg tggaaagcca tattcacaaa
    17221 atttttctta ctactcgaaa ctaagcggct atttcacaaa ctcttggtaa acggtgttca
    17281 atcagctaac cctcggtatt cgctcacaaa acgacaaaat atgaggaact atttttccgt
    17341 ttgtttctct aataatcagg tcaaggtgct gattgcccaa cctttttttg tataatgaga
    17401 aagtataaac ttaatgtttg gaggagaaga tatgttaggg aaggttttgg aagagttatt
    17461 tatccggatc tgggtcatta taaaactaac cttgtatttc tggatatata catttgccgg
    17521 cgggatcatc tttggattag gtgcggcatg gaaaacagtc aatgaactat tttatttgta
    17581 cggattcgag tataaagaaa tcacaataaa aagaggctgg aatatatata aaagaaattt
    17641 tttaagaggc aatctgctat ttagtttatt tttatctggt actgctttat tgagttacaa
    17701 tttatattta tctgttcaaa tcaaagggct cattttttta gctattgatt ttattttact
    17761 ttttgccttg ctctgcctat ttgctactta tcaatacagt atgattttag atagtgaata
    17821 tacgatctct gttcctaatc tgctaaagct aagttttatt tcggtttttt ccagcttttc
    17881 gtcttttcta aaaataatta ttggtagcgg tataatttta ggcgtgactt ggcaattcaa
    17941 aggattgatc ctgtttggtg tcattggtct tttgacggtt tggaatggta cgatgacaac
    18001 acattggcgt gaagaattag ataaacagtt ggaaagttat gaataagttt cggattctat
    18061 tttcaaaagg aaccttacta aatcgtctgt tgcggaaata cgcggttatc atgatcggtg
    18121 taacgatgac tgctaccgtt atttttagta tccatacttg ggaacaaaat caaaaacagg
    18181 cagaaaacat gacaagtgat gccgttcaaa gcacaagcag aatgctgaat gataaaacaa
    18241 ccttgagtag gatcatcaaa aaccagcttg tagggaattc cgaaaagatc gaaaatgtga
    18301 cgacctactt aacaaaaccc atagatcagt atctgatgta tgtatacgaa cagcaaaaca
    18361 gtaccgatga attagtttct ttcccaaatc aaatcaaaga tctctacgca aattatgaag
    18421 aactaagtgc gatatatatc gtgttgaatc aactaccgga gtattatgaa tcttctagag
    18481 aaaacaaagg tggggaaata aaaaagggga caccaaaatt actagacgcc ttttatatca
    18541 agttgcctat cacacaagga ggagctgagt caggaagtat ttttattggt tttcaaaaga
    18601 aagaactaga tatgatttta gaaaatctaa cgtcattcaa tggactctct ttatacatga
    18661 tttctggaac gacaaatcga ctatacacat tccatgataa taaaatagct aaagaaacgt
    18721 ttgaaagaca agaaatgatc atcacagaga gtctgaagaa aaattcacaa ttgccgattg
    18781 acgatctgga aaaaaacaat tttgttcaac accaagaact gtctgatgat tatcgtatat
    18841 tagctgtact agataaaaaa agtgtaaaaa tcgaaacggg aaaaaatctt gccccgctga
    18901 tcattggtat cattatttta gacaatatac tgcttatctt tctttataaa acattcaaac
    18961 gatattcaca gcaagtatct atcgtcatgc aggccatgga tcaaacagca gcagggaatt
    19021 tagagacgcg gatcgacata acaaacacgg aatatgaact gagggaactt tcaatcggta
    19081 tcaacgaaat gttggatagc atcaatcagt ttgtagaaga catctacaaa ttagaaatca
    19141 aacagcaaga cgcacatatg cgggcgcttc aagcacagat caatccacac tttttatata
    19201 atacattaga atatatccga atgtatgcaa tcagcgaagg aagtgaagaa ttagcagatg
    19261 ttgtgtatgc gttttccgct ttgcttcgaa acaatacgaa tcaagagaaa acgatcactt
    19321 taaaagaaga actggacttt tgcgaaaaat atgtctattt gtatcaaatg cgttatccta
    19381 atcgagtagc ctatcatttt atgatcgatc ctgatttgga aaagatcgaa gtacctaaat
    19441 ttgtgatcca gccattagtt gaaaattatt ttaaacacgg tattgatttt actcgattcg
    19501 ataatgcgct aagtgtcaaa gtcttgcagg aaggcaaacg ggtaaggatc atcatcaaag
    19561 ataatggaaa agggatgact gaaaaacggc taaaacagat tgaagaaaaa ctgtctcatc
    19621 ctaaagtgga acttcacggt tctatcggtc tgcaaaacgt gaatgaacgt ttacgtgcca
    19681 gcttcggcag cagttattat atgtcgcttg agaataatga gacaggtgga ctgaccgttt
    19741 ctattacatt caaggaggga tgagcgtgta tcaagtgtta ttggtagatg atgaatacat
    19801 gattttgaat gggttaaaaa agataattga ttggcaaagt ttaggctttc aaatagtagc
    19861 aacagcagag aatgccaaag aaggactagc tgtacttgaa cagcgacaga ttgatttagt
    19921 agtaacggat gtgacaatgc cagaaataaa tggactggaa tttattgaag cggctcaaaa
    19981 agaacgtcat aattttgaat ttatgatttt atcaggatac caagaattcg attatttgaa
    20041 aggaggtatg caattaggcg ccgtgaatta tttgatgaaa cccgtgaata agttcgaatt
    20101 ggttgaaagc ctcaagaaaa tcaaaacacg gttagatcag caaaatgaac aaaaaaatca
    20161 acaagaaatc tatcaagaac tgttattcag tcaatggttg aatgaagaat tggatgaaat
    20221 aagtgaagaa gaaataatag gacaaataag tgagaagcag cataggatag tattgattca
    20281 actggcaaga actcatgata ctcttatcaa tagatggttg caagaacggc aacaaatctt
    20341 ttactatcaa cgaaattacg gagaccgtgt attgatcacg ttgttgttgg aggcagatga
    20401 agttgatggc ttctgttgtt ttgttcaaga aaactttcct gatcaggaat ggctgatcag
    20461 tatcggggaa gagacagatt ctttggacaa aatccctgaa acattccaac aagcgaaaga
    20521 caatctacag ttgcatcaat tttatggaga taaagacgag catgtttttt acgcagtaca
    20581 agcagaaaca aaagatcaaa ccatcgattt ttcaagtttc agacgcgtgc tgcaaaataa
    20641 acaattggaa gtcgctcaaa aaatgatcac tgattttttt gaacagtttc agttagcagc
    20701 catgatgccg gaagatatac gatattccag ttttttactg tttatggaga ttcaacggga
    20761 attgattgat ctggaagatg aggaatattt acaaggaatc gaaaaaatcc atcaggcaaa
    20821 aacggtgcaa gaacttcagc agctacttct gtcattcatc aaaaagcatc agagacagaa
    20881 aaagtacagt gacaatgtgg aaaaagtaat cgtaatcctg caccagcatt atcaagaacc
    20941 acttacactc aaagaagtgt cagaaaacct gcatttaaat gtcatgtatc taggacagct
    21001 gttcaaaaaa gaaacgaaaa aaagtttttc agcttatttg aaccatttac gtatggaaaa
    21061 agcgaaacaa ctgcttttgc acagtaatca aaacatcaat gagatagcaa gtgagattgg
    21121 ctacaacaac accacgtatt tctctaaatt attcaagaaa atcgtcggca gatcccctaa
    21181 agagtaccga gaaaacagtg gagaatcata aaagaaaaag ccaatgtaat gaaataactt
    21241 ataaaaaatt gtctagaaat ctaattttgg gctgttctta ttacttgagg caaggcagct
    21301 ttttcatgac ctctcgataa acggagttca aaagccagtt tctcggtatt aagccaaatt
    21361 tatgcaaaaa tgtggaaagc cattttcaca aaatttttct taatactcga aactaaacag
    21421 ctttttcacg acctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa
    21481 aaaatggcaa aatatgagga actattttgc catttgtctc tctaatactc aggtcagaac
    21541 gctgatttca caacctctca ataaacggtg ttcaaaagct tgcatctgcg gtaataagac
    21601 caaccaaacg aaaaatatga ggatctattt ttgcttggct gttcttatta cttgatgcag
    21661 gacagctttt tcacaacctc ttgttaatta tagtttgata aggaaaaagg caaatctttt
    21721 gcttgatatt tcttagggtg agggatgatc cctaattggt aagagatcgt gccgcctttc
    21781 agcaaatctt gatgggtaaa atataatttc gtatattctc tgtccatccg tttgatttta
    21841 tggataaatt gttgctgcgg ccgattaggc gttgcttcaa tcgtcaattc ctttccattt
    21901 gacaaaagca aaacagcctt gtcaattaat ggcataccga taacatattc accggttcca
    21961 ggcgtgacag gataaaatcc cagactattg aagatatacc aagcggccat acttccattg
    22021 tcctcatctc cagggtaacc cattggagaa tcgtcgaagg tctgagtcat caactgttta
    22081 agcagtggct gtgccatttc aggttgtcct atataactga acaagtaagg atagtgaaaa
    22141 cttggctggt ttgaaatagc taattgtcca aattctatcg cggccatctc actcatttcg
    22201 tggatctcaa aaccatatcc tcctgggtca aacgttgggg gctgattgca cagctcaatg
    22261 agtttttctt tatatctctc actgccacca tattttccga tcagtcctga aaaatcgtga
    22321 taaacagcaa agctgctttg ccaagcactt ccttctgcat aatcttgtcc ccaacgatta
    22381 gctgaaaatg gttctctgaa cttgccatct ttatcttttg aacgcataaa tcctgactca
    22441 tagtcaaaga tattttgata gttcaatgat tgctgctgat atttcttcgt catttcctct
    22501 tcttctagta ccttggcaac ttggctaata caaaaatcgc tataagcata gtctaatgtg
    22561 tgattgactg attcgtggta gtgattagga acatagccat atgtcaagta atcttctgtt
    22621 ccttggcgtc cataatttgg atgtttgctt tgaaccgtag cacctttttc cattgccgtt
    22681 agaaattcag gcatcaagtc tcgtcggata tcttttacag cagcatctgc aatgaccgca
    22741 tcgatcaatg tcccaggcat aagtccacgt tcatcaggag acagccattt tggcaaatag
    22801 ccactttctc tatagctgtt caaaaaacct tccagcattt cttcgtattt ttctacagca
    22861 atcaaagaat aaagaggata aaccgtttta tacgtatccc aaaacccatt atttgtatag
    22921 agaacaccag gttttataga tcgactagtc gtatcatagt ggattttttg cccactagga
    22981 tcaatttcat aaaatgtttg tggaaataaa aacacccgat ataaattatg ataaaacgtc
    23041 cgcaattgtt gttgatcatg gtgtgtgagc tgtatacgat caaaatagga ctgccattgt
    23101 tcttgactgt gagcaaggta agtctctttg ctttggtttt gttctagttc caggttataa
    23161 gctgcttgtt ccgagctgat aaaagatgtc ccaaatcgga tagtttgttt ttttgtctgt
    23221 tcaaaataca acgggatcga gtcgctattt ttgttggaaa cagctaactc atcaattgtt
    23281 aaaggatatt caaatctcaa tataaaataa aaagaaaaat caggatcttc tgaaccagca
    23341 aaatgtgtga tttttccttc cactgtgtaa ggatctataa caaagagctg atggcgtccc
    23401 ggaaaggata aaagtaaacc gctgtcattt ttgctgtaat caatagataa gattccgcca
    23461 tacatgctag gtatcaatgt agaacagatg ccataacgta attgacagat cgacagatga
    23521 gtcgggcaaa atatactttc ttctgggcgg taagaacttt gagcatggaa aagggtgttc
    23581 tcctgtagtt ttccattgat cggcgtaaac agcatatgac tgaaatcacc catccaagga
    23641 cttggttgat gggtaagacg gtagccttga aaaacgcggt catcaggatg aaaccaccag
    23701 ctgccgtttt gatccgtagt ttgcggtgca aaataattca tcccaaaagg aacgccagta
    23761 taaggctggc agttcccatt agagaaattt ggctgattcg ctgttgcatg tctagtatct
    23821 atttgaccga tattcatttt atttctcctt tgttgattgt cttttgatga atgaaactgg
    23881 aaatttacta tcggatggtc gatcttttgt ctctaaccaa gtgaccaaag catctcccgc
    23941 aagcttacct aactgttcaa aatcttgtgc gatggttgtc agtggcgggt caacaagagc
    24001 tgctgcttgg atatcatcaa atccgataat tgaaaaatct tcagggatat gataaccatg
    24061 ctgtctcaag agattcatgg tctggatagc gacaacatcg ttttcacaaa caaaagcagt
    24121 tacttcatac ttagtgatat aagaaagcaa ttcatctaat gtcgcgcttt tgtcatccag
    24181 tgaagtgtgg aaagtcaaac cagctttgtc caatgcttca aggtaaccaa ggtaacgctg
    24241 tcgaacggat tgtggatgag tctgcgtacc aaacaaataa gcgatttttg aatgtccctg
    24301 ttcgatcaaa taagaggcag caagcgcacc gccttggaaa ttatcggaaa gaacggtagg
    24361 aaaaggtaac tcatagattt ttttatctaa tatgacgaca gggaattctt tcaatgaaag
    24421 ctcaaaaagt aagtcaagat gttgttctgt atcaagagca taataaatca atccgtcaaa
    24481 ttcattgatt agctcttttg ctgttttatt ttgtagaaag tcaagcgtcg tcatcatgac
    24541 atcaatctgt ttatcttgca tcacagggtt caatcccttg ctaaaatcac caacagacaa
    24601 atcagcgaca tatggaagaa gaaacagaat ccgtcgtgtt tttgtaggag aagcaagaat
    24661 ctgttccgga gaagcggatt taacgaaact gcctttcccg cgaacgcgat aaataagtcc
    24721 taattgctct aattcagtca atgcacgttt ggaagtgatt ctgcttactt tataggtacg
    24781 tgaaagttct ttttcagttg gtagttggct gtctacaggt aattcaccag ttaaaatagc
    24841 cgtttgcaaa tcattaaata ttgtctggta caatggttga gtcatataaa cacctcttaa
    24901 tgatatatca tattcaaata ataccataat ggatgaaaaa gtaaataaaa agatattttt
    24961 tgaaagcgta gacataattg gctgtatgaa gtataatctt taaagatata tcaaatagaa
    25021 agaaggtttt attagtggtt tataaagata taccaaaatc ggtgcagaaa tttatggaag
    25081 atatcacaca aaaatgtggg gatatccatg cggactgggc aaagaatttt acagcagcat
    25141 ttgccaatac tctgttgaca acagtcaaaa agcaaaatga cggaaccacc tttttattga
    25201 cgggagacat tccagcgatg tggttaagag actctactgc acaagttcgt ccttatttag
    25261 tgattgcaaa agaagatgag gaattagctg acatgatcag cgggttagtc aaaaagcaat
    25321 ttttttatat caatatcgat ccatatgcga atgccttcaa cgaagaagct aacggtgctg
    25381 gccaccaaac agatcataca aaaatgaacg attggatctg ggaaagaaaa tatgaaattg
    25441 attctttatg ctacccaata cagttggcct atcttcttta caaaaataca ggaaaaactg
    25501 atcaattcaa tcaatctttt attgaaggta tcaaaaaaat cttggacgtg ttcaaaacag
    25561 agcaagatca tgaacactct ccttatacat ttgtacgaga cacgacaaga aaagaagata
    25621 cgctgatcaa tgacgggcga ggtgcaaaaa cagcttttac aggaatgaca tggtctggtt
    25681 tccgtcctag cgatgatgct tgccgttatg gatatctggt accatccaat atgtttgcgg
    25741 tggtcgtgtt aggttatgtg gaagaaattt ttcaaacgat actcgatgat aaagaaattg
    25801 tacagcaaac acagaaattg aagaaagaga tccaagaggg gattcagaat cacggacgaa
    25861 cggttaatca aaaagggcaa acgatttatg catatgaagt agatggaatg ggaaacgcca
    25921 cagtcatgga tgacagtaat gtgcccaatc tgatcgccgc accttacttg ggatattgtt
    25981 ctgaacaaga ggaagattat ctgcaaacaa gaagaacatt gttgagtaaa gaaaatccat
    26041 atttctatga aggaaagtac gcaaaaggaa ttggatcctc ccatacgcca gaaaaatacg
    26101 tttggccgat tgcattagct atggaaggaa tgacaacgtc agacaaatct gaaaaagagc
    26161 ggatattgaa tttacttgta gcaactgacg caggaacaca ccttatgcac gaaggattcg
    26221 atgtgaacaa tcctgaaaat tatacgagag aatggttttc ttgggcaaat atgatgtttt
    26281 gcgaattagt catggattac tttgatatta gagtagaaaa gtaggaggta tcgtaaatga
    26341 aaaagaaagt atatatcatt tctcatagcc actgggatcg tgaatggtac atggcttacg
    26401 aacagcatca catgcgactg attgaattga tagacgatct actagagcta tttgaagttg
    26461 atccgtcctt caacagtttt catttggatg ggcaaacgat tattttagat gactatcttc
    26521 aagtaaggcc agaaaaaaga caggcaatcc aacaagcaat caatgaagga aaattgagaa
    26581 ttggaccatt ttatatcttg caagatgatt ttcttatcag ctcggaatcg aacgtgagaa
    26641 atatgctgat tggcatggaa gaaagccgta aatggggaac ccctgtcatg ttaggctatt
    26701 ttcccgatac atttggaaat atgggacaga cgcctcaatt aatgaaacaa gctggtattt
    26761 cagcagcagc gtttggccgt ggtgtaaaac caattggctt tgataatcaa gttttggaag
    26821 cagaaaacta ttcttcccaa tactcagaga tgtggtggaa aggccctgat caaacagcaa
    26881 tctttggtct gctatttgcc aattggtata gcaacgggaa tgagattcca gtagaaaaag
    26941 aagcagctct tgccttttgg aagcaaaaat tagcggatgc agaacaatat gcctctacga
    27001 accacttatt gatgatgaat ggtgttgatc atcaaccggt acaaaaagat atatcaaaag
    27061 caatccatct cgcaaatgaa ctgtttccag attacgagtt tattcattct aattttactg
    27121 actatctgga agcagttcaa aaagatgtgc cagaagactt aggctcagta gaaggagaat
    27181 tgacaagtca agaaacggat ggctggtata ctttagccaa tacagcatct gctcgggttt
    27241 acttaaaaca atggaatacc aaggtccagc gacagctaga aaatattaca gaaccacttg
    27301 cgacgatggc ttatgaagtt agtggaaact atccccatga tcaattagat tatgcttgga
    27361 agacattaat gcaaaatcat cctcacgaca gtatttgcgg ttgttcagtc gattctgtcc
    27421 atcgggaaat gatacctcgt tttgaaaaag cagatgaagt tggaaagtat cttgcacaag
    27481 attccttaga acagctgacc gcagcaatcg atacgaccgg attcccaaaa gacagctttc
    27541 catttgtcat tgtaaatact gcaggaatgg ataaaactgg tgaagcagaa ataaccatcg
    27601 aattagaaag aaaaagattc gctgaaggaa tacctgaaca actatatcaa gagttagaaa
    27661 atttgcctaa acgtaagtat catgtagaaa caaaaagtgg ggccactata ccagctattt
    27721 tatctgaaga aactgtccaa tttggttatg atttgccgaa agaccgtttc cgtgtgccat
    27781 atatggcaag aatgatcaaa gtgactttac cattagaaaa catgccagca ttttcttggg
    27841 aaacattcgc gcttgtagaa ggggaagcga aagcagaaga aaaggaaaca atgattcatc
    27901 agagtggacg aatcatcgaa aacggccctc tacatttgac gattgaaaaa aatggtacca
    27961 ttacaatgga agatcgcaaa aacaaacgaa agttgaataa tctgcatatt tttgaagata
    28021 tcggcgatat aggaaatgag tatatcttta aacaaccttt ctgtgatcag ccaatcctat
    28081 ccagtaacaa agaaaactct gaagtaaaag tattagtaga tacaccggaa atagcaaaaa
    28141 taagtattct acaggaaatg gaaatccctg tttctgcaga cgagcgttta gaaaaagaac
    28201 agcagatggt tgtggaattt cgttatcgaa aagcagaacg atccaaagaa aaacgaatcc
    28261 ttcaaataaa aacaatcatg acaatcagaa aagatagtaa aaaaatcgac tttgaaacga
    28321 ctctcgacaa tcagatgaaa gatcatcgct tacgtgtctt gtttcctaca aagttacatg
    28381 tggaacatca cgaagcagat agtatttttg aagtggtgaa gcgcccaaac catgtgtcta
    28441 aaagttggga aaaccccacg aatccgcaac atcagcaagc atttgtcaat attcatgatg
    28501 aagagtacgg tgtaacagtc ggaaactttg gtttgaatga atatgaagta acagaagacg
    28561 gacaaatcgc agtgacgcta cttcgcagtg tgggagaact aggcgattgg ggatattttc
    28621 caacaccaga agcacagtgt ctaggtgagc atcggttcaa ttacagcatt gaactacatg
    28681 gaccagaaga aaaattctct acttatttgc atgcatacgc agcacagatt cctttttcca
    28741 ctcaacaaat caaacatcat gaaggaacat tgatttcaaa acaacaatat ttaacgataa
    28801 aaagtgaaac gtttgcaatt acagctttga aacgcagcaa gtttagtgat aaagtagtag
    28861 taagaggttt caatatgtct agtcatttag aaaaattgga aataactaaa gacaatggaa
    28921 aaactgtcat attgaatctg cttgaagaac caacgaagca agcggttgtc ccaataatcc
    28981 aaccttatga gattcggaca atcggattcg aggaggaaaa cgaatgtatg gcggaataga
    29041 agcaggcgga acaaaatttg tgtgtgcggt ttcagatggt ggagaaattt tagaaaaatt
    29101 aagtatccct accacaacac cagaagaaac attggatcag gtatttgaat tttttgatcg
    29161 ttatgaattg aagggaatcg ggattggctc attcggcccg attggtatcg acaaaacaaa
    29221 agataattac ggatacgttt tggcaactcc caaaaaaggg tgggcacaat tcgattttct
    29281 tggcacaatc aaggaacgct acaatattcc tgctgcttgg acaacggatg tcaatgctgc
    29341 tgcatatggg gaattgaagc aaggagcagc acaaggaaaa aatagttgta tttaccttac
    29401 cgtgggaaca ggaatcggtg caggtgtcgt gatcaatgaa gaaatttttt cagggatcgc
    29461 acatcctgaa atgggacata tttgggtgaa acgccatcca gatgaccatt atgaaggtac
    29521 ttgtccttat cataaagatt gtttagaagg attagcagca ggaccgtcta ttgaggcacg
    29581 cacagggatc aaagggcaag atctgccgca agatcatcct gtttgggcta ttcaggctta
    29641 ttatattgct caagcactga tcaactatac gttgacgtta gcacctgaaa agattatttt
    29701 aggcggtggg gtaatgaatc aggatcatct ccttcaaaaa atccgtcagc aatttgttga
    29761 attgatgggt ggttatatgg aaacacctca agtaagtgaa tatatcgttc gttggggact
    29821 gccgaatgaa agtgggatta tcggtagttt gctgttagca gaacacgaac atcaaaagta
    29881 agcagacaaa ttacacaaaa agaacagagt cttatcatta tctgctgata agactctgtt
    29941 cttttttatt tctattccca taatccaggt aaagctttac cgactatttt ttcttgagta
    30001 aatggatgga tcatgtgcag ttctaatgca tggagcatca gtcgttcagc tgttctattt
    30061 tggtagagag ggtctccaac gatcggatgt cccttatgag ctaaatgaac tctgatctgg
    30121 tgtgtacgtc cggtttccaa aacacaatag acttgtgttt gttcagaaag tactttgttg
    30181 acagtgacat gtgtcaaggc gctctgacca tttttatcat ctacgaccct ttttctccga
    30241 tcatggcgat ctcgtccaat tttattggtg atggtcatat ctttggcaat ttctccccat
    30301 acgatggctt gatatctccg atagatcatt ttttgttcca acattcttcc aagtatcggc
    30361 aagacaaatg gattttttgc aaataaaacg gcaccgcttg tttctttgtc caagcggtgg
    30421 acaacgtaag gtcgttgctg tttagtgtcg agataagcag ctaaatgatt gagtaaggta
    30481 ctgttttctg tcggttcatt tggatgagtc ttcattcctg caggcttatc cactataatc
    30541 aaatgctcat cttcatataa aacacggatg tgcttcgcat ctcctaattg aacttcttga
    30601 taattataat ctgtttcttc gaataacaat gtgatctggt cattgccctt tacttcatga
    30661 tggaacaaag caggttgttg attgatccaa acgtttttac gtatacgcaa aaagtgtcga
    30721 acctttctag gaactaacca ttccttttca agcaagtcgc gaattgttgt ttcaggatgg
    30781 ttcataggta aagtgatcgt gattttcaca agtgtactcc tttcagtgca attgcagact
    30841 aattgtacca tttttaaaac atatttaaga aaaaacttac atttttctgt tcattcttat
    30901 gttactctac ttgcactaac gtaaaaaaac atattgaact acaacttaag caggaagaaa
    30961 acactctttc actatggtaa aataagacga ttgaatcaaa tagacaggag aagaagcaaa
    31021 tggattttca aagcatttta gaaaagataa aagctgcttt gatcagtttt tggcgttgga
    31081 tcagaccata tctcggacaa ttccatcgat ggcgaaagcg catctggaaa aaatatcatg
    31141 tgaacaaatt gattttgctg attggtttga tctgtgtctt agtgacaagt atctacctct
    31201 ttattttagc aaaacaagca aatgtcgaaa cgttaaaatc aggattaagc caatcgacag
    31261 tcatttatga taagaaaaac gaagaagctg gcacactgta cgcccaaaaa ggaacatatg
    31321 tagaattaaa cgcgatttct ccctatatcc aagatgcagt aatctcgaca gaagatagga
    31381 atttttacga gcatcacggg tttgatatca aagggattgc ccgtgcagca gtccgaatgg
    31441 tcttgtctgg cggaacatca gggggcggag gaagtacgat cactcagcag cttgccaaaa
    31501 atgcgtatct gacattagac caaacgtttg atcgaaaagc gaaggaatta tttttggcaa
    31561 tcgagatcga aaagaagtac agcaaagaag aaatcttgac catgtatctt aacaatgcct
    31621 attttggcaa cggggtatgg ggtgtccaag acgcttccag aaagtatttt ggagtagatg
    31681 caacgaatgt tacgctgtct gaggctgcta ctttggcagg aatgctgaaa ggtcctggta
    31741 tttataatcc gatcgatcac ccaaaaaatg cgaatgatcg gaaaaacaca gtactaagtg
    31801 taatggaaga aaacggaaaa atcacaaaag aacaagaaca agcagaaagc cagacagata
    31861 tcagcgcgta tcttaatgac acatacgata attcagattc tggttatcgt tatccttatt
    31921 attttgatgc agtgatcgat gaagcagtta acgagtatgg tcttgatgaa gaagatgtca
    31981 tgaataaagg ctataaaatc tatacagctt tagatcagga ttatcaaaag caattagaag
    32041 ctacttatca aaacagtgca ttattcccag caaatgcttc agatggtgcc atggtccaat
    32101 ctgcttcagt agctttagat ccgaatacag gtggtgtaca agcactagta ggacgaagag
    32161 gagatcatgt ctttagagga ttcagctttg ctacacagat gcgtcgttca ccaggatcaa
    32221 cgatcaaacc attaagcgta tatacaccag cactggaagc tggatacaag ccttccagta
    32281 tcttgaaaga cgaaccgcaa agttattacg atgcgcataa ttttgacggg acataccaag
    32341 gagaagtacc gatgtatcaa gctgttgcac aaagtttgaa tttgcctgct gtatggcttt
    32401 tgcatgagat cggtttacaa aaaggatatg ataaagcaga agaattcggc ctgcctttag
    32461 caaaatctga taagtattat ggcttagccc ttggtggact ggaaaaagga gtatctccat
    32521 tgatcatggc gggtgcttac agtgcatttg ccaatgatgg tcagatgtat acgccgcatc
    32581 ttatcacgaa aatcgttgat tcgactggag cggtgatcgt cgataacaca aacaaaaaac
    32641 caaaacaggt catatcaaag gaaacagccg atgaaatgac cagcatgctt cttggtacat
    32701 tttcaaatgg aacagcggct gctgcgaatc ctgcaggtta tacgatagca gggaaaacag
    32761 gaaccacaga aactaatttc gatgccacaa aagcaaatga ccaatggatg atcggttaca
    32821 cgccagacgt agtgatttcg acatggatgg gttacgaaga atccagcaag ctgcactatt
    32881 tggaagggac aagtggaaat gttgtcggga aagtcttcaa atcagcggca gaaggcatcc
    32941 ttccgtatac ttcaaaaaca ggatttagtg tagcagatgc ctatgccaca ggaggacaag
    33001 tggtagctgc agatcaagtt ggaaacacag gaaattctaa tggagagtct ggaaattggc
    33061 aagataactt gaatcaatat ggtgaacaag caaaagaagg gctgaaaaac ttcggcgata
    33121 tggtgaaaga aggcgttcag ggaattggtg aagcggctaa agacctctgg cggaaatatc
    33181 aaggagagta gcccctttga aaacgaataa ccccatgtta caatagagag taactactaa
    33241 aagaaaagag ggcataacat gtcaaacatc tacgatagcg cgaatcaaat cgaacgtgag
    33301 atccgtgaat taccagaatt caaagcgttg aaagaagcgt acgagaaagt acaagcaaac
    33361 gaagaagcac acaaactgtt caaagacttc caagcgatgc aaatcgaatt acaagaaaaa
    33421 caaatgagtg gacaagagtt cagcgacgaa gatgcaatga aagcacaaga aatggctgtg
    33481 aaaatccaat cagaagaagt aatcaatgac ttgatgcaaa aagagcaagg cttcagtaca
    33541 atcatcaatg acttgaaccg tatcatcatg acaccagttc gcgaccttta cagcgaataa
    33601 aactatttta tgaaacaaca gagggtgtga aagagacgtt cggccttgag cgatgacaaa
    33661 attgcttctt attgccgata ggctgtctct tgaacaccat ttaccaagag gtcgtgagcc
    33721 taccgtttaa ctctgagtta caaggaacaa tttcaaaaaa cagcttctca tgtttgatga
    33781 ctattgtgac ttgtgaccga ggagttggca ggagaacacc gtttattcag aaataggagc
    33841 tacgacaaaa gtcttgtcat agctcctatt ttttgtcatt catttttcaa gcggtagaaa
    33901 tagccttttt gagctaacag tttttcgtaa gtacctattt caacgatttt tccttcatcg
    33961 atcacaacga ttttatctgc ctctttgatc gttgaaagcc gatgagccac aataaaagta
    34021 gtgggtgttt ctgtcaggta attcagggct tgctggattt ttttctctga ctggttatct
    34081 aaagctgagg ttgcttcatc tagcaaaatg atttttggct gacgtatcaa cgctcgtgca
    34141 atggagatac attgcttttg gccaccagac agcttgttgc catgttcgcc tactaaggta
    34201 tccagaccat caggaagctg gtcaatcagt gaagaaagtt gcgcagcttc gatgacttca
    34261 tctaaacgtt cttttgatac gtttttcaag ccataagtga tgttctcttt gatcgaagcg
    34321 gagaacaaca aagtggtttg aggaacaacg gctaaataat ttcgcacact tcgcatatcc
    34381 agttcatcca aaggctgatc atctagaaaa atttttcctg aagtcggctg gataaatcca
    34441 atcaataagt taaggagtgt agattttcca gagccagatg gcccaacaaa agcaattgtt
    34501 tctccagctt tcacctctag agaaaaatga tcgataatcg gtttgagtga gtccggataa
    34561 agaaaagaaa catcctcaaa acgaaaattt ccatccaaat gttggatttt tttcttaccg
    34621 tgataagttt ctacatcgcc agcaatcaac agttcgccaa tagaagtgat agattccatg
    34681 ccttttgaaa tcgtaggaat cagcgtgatc attccagaaa cggaattgac gatcgtcgtg
    34741 aaatacgtct gatacatcac gacatcgccg ggttggatgc gcccttgtaa agctaatatc
    34801 ccagtgaaaa tcaaacaaat cagctgaaat atctgaaaac tagcccagct gactgatccg
    34861 aaaacagatt gcaggatatc taagtggaat cctttttcat agatttgagt aaaatgctta
    34921 tttaagcgag ttgtttcgtg tttttctaac ccatgagctc gagcaatcgg tgctaattcg
    34981 atcacttcga tcacttttgc agaagtctct tccatttctt gcctgaagtc tgagttggac
    35041 tgtctgatcc ttttacggaa aaagagagta acgaatgcgg cgagaggaac agtcaataag
    35101 aaaaacatga aaacaacagg acttttataa agcgtgatac ctaaagctac gaataaattg
    35161 acagcgatat ttaatccgct gacaaatact tgagaggaca acgtttggat agcttctaca
    35221 tcacggatga tctttgactg gattcgtcca gattgcaatt ctttttgata aggaatagat
    35281 agttcttgga ttttcttgat aagggtcgct cttagtccag cttctacttg acggatcgaa
    35341 gtgctatggt attttacatg aaaataattc gtcaatatat tttgaccaat cagacaagcc
    35401 atcaaaaaag cgttgaaata aatggcctgc aactgagaag aatctttcga tgaagctgca
    35461 ttgattacat tggcaatagc gatcggcatt aaccaagatg gaagatgttt gataataaaa
    35521 aagagtcctg acatggcaaa acgccaataa tttccctgat aaagatgcag caatgtctga
    35581 aaagcatttg catgatattt ttgataactt ttctgtattc gttcctccat aatcttctcc
    35641 ttaacaatct tggtaaataa actgcaagtg ttctattgct tcaggaacat aaatctcagg
    35701 agtagcttca agagtggcgt acattagagg acgctcatat ttgatataat gcagtaccgg
    35761 gcggaaatct aaacagccct tgccaacagg taccatgcgg atctttccat tttccatgat
    35821 atagtctttg agatgaagcg ctgtaatata accatgtaaa ttgtccaagg cacctcggat
    35881 cactttttcc tgatcagcat gattttcttt atgcatcaaa ttcgcacaat ctaaaatgat
    35941 ttttaaattt ggtgaagcta cttcatcgat taaacgttta gctaactgat agttatagat
    36001 tggatgattg atccctgctt caacagcaac agttacgcca aaattttctg catgagcgac
    36061 taattcaatg atagaatttc tggcgatctg ataagcttct tcagtaaaat tttcttttgt
    36121 gtaaccgaca gagacactac cggtttccgt agcaatcatc ttggcttgat aggcagagca
    36181 cagagcaatt tgctgtttga aagttgccaa tgcttgtgca cgaacttctt gatttttgct
    36241 ggataggttg atatagcatc ctaaaatgga aagttcgata ttgttttttt ggaagtattc
    36301 accaaaatag gcagcactcc caagtgtcat gtcagttaat ttaagttgtt caggaaatgc
    36361 tttttgtggt gcaaattgga tatgggacaa gtgaagctgt gatacttttt gaacgatttc
    36421 ttcacgattt cgaacatcta aatcgtgggc acggatgcct aaatttaatg gaaacatctc
    36481 aaactccttt ttgtaaataa ttagcaagag gcagacggga ttcttttatc aattcaacaa
    36541 ttattcgggc aactttatca gcgccttctg gactaaagtg ggtattgtct tttagtcctt
    36601 ctgggtaatt tttacaagta tcaggcataa gatgcaggta gaattttttt gtctcttctt
    36661 ttgagaaggt ctggaacaat tcttgtgatc ttgagaacac atctaaaaga ggaatatggt
    36721 gtttctcagc aaatgcacgc atcgcttttg gatagtctcc aagtatatct ggattcaatg
    36781 ttccgttttc taagtaatct cgtcgcgtca cagatgtcag taagaccatt tggacgttcg
    36841 ctttttttgc gcattcaaaa aatatctgca gattttttaa atagctaccg aagggaagcg
    36901 tacctctttc ttcttggatt ttctggtcgt tatgtccaaa ttgtacaaat aagaaatcat
    36961 tcggctgaaa ctgtgaagct agacgggcta accttccttc ctggataaat gatttggtgc
    37021 tgcgtccatt ctttgcttgg ttattgatct ggatctcggg tgaaaaataa gcagagattt
    37081 tttctcccca acctgtctcg ggacgcttgt tttcatcttt ttctgccgca gtagaatcac
    37141 cggcaatatg gatagtaggc atgttatctc ttctttcata aaggtttata ggcaatgaag
    37201 cagaacagca agagaagaag acggagcaa
//
LOCUS       DACDTO010000018        35050 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDTO010000018 DACDTO010000000
VERSION     DACDTO010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.018"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            173..1309
                     /locus_tag="IWK70_000965"
     CDS             173..1309
                     /locus_tag="IWK70_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9613531.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            1446..1790
                     /locus_tag="IWK70_000966"
     CDS             1446..1790
                     /locus_tag="IWK70_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9613532.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            2061..3347
                     /gene="efmA"
                     /locus_tag="IWK70_000967"
     CDS             2061..3347
                     /gene="efmA"
                     /locus_tag="IWK70_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9613533.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(3485..4084)
                     /locus_tag="IWK70_000968"
     CDS             complement(3485..4084)
                     /locus_tag="IWK70_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9613534.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            4488..5609
                     /gene="mnmA"
                     /locus_tag="IWK70_000969"
     CDS             4488..5609
                     /gene="mnmA"
                     /locus_tag="IWK70_000969"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9613535.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            5855..7276
                     /gene="arcD"
                     /locus_tag="IWK70_000970"
     CDS             5855..7276
                     /gene="arcD"
                     /locus_tag="IWK70_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9613536.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            7290..8408
                     /locus_tag="IWK70_000971"
     CDS             7290..8408
                     /locus_tag="IWK70_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9613537.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(8468..9760)
                     /locus_tag="IWK70_000972"
     CDS             complement(8468..9760)
                     /locus_tag="IWK70_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9613538.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            9910..10470
                     /locus_tag="IWK70_000973"
     CDS             9910..10470
                     /locus_tag="IWK70_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9613539.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            10599..10976
                     /locus_tag="IWK70_000974"
     CDS             10599..10976
                     /locus_tag="IWK70_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9613540.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            10909..12981
                     /locus_tag="IWK70_000975"
     CDS             10909..12981
                     /locus_tag="IWK70_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9613541.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(13192..14139)
                     /locus_tag="IWK70_000976"
     CDS             complement(13192..14139)
                     /locus_tag="IWK70_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9613542.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            14385..14849
                     /locus_tag="IWK70_000977"
     CDS             14385..14849
                     /locus_tag="IWK70_000977"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9613543.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(14911..15954)
                     /locus_tag="IWK70_000978"
     CDS             complement(14911..15954)
                     /locus_tag="IWK70_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9613544.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(16331..17296)
                     /locus_tag="IWK70_000979"
     CDS             complement(16331..17296)
                     /locus_tag="IWK70_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9613545.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            17530..18375
                     /locus_tag="IWK70_000980"
     CDS             17530..18375
                     /locus_tag="IWK70_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9613546.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            18566..19753
                     /locus_tag="IWK70_000981"
     CDS             18566..19753
                     /locus_tag="IWK70_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9613547.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            19759..20115
                     /locus_tag="IWK70_000982"
     CDS             19759..20115
                     /locus_tag="IWK70_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9613548.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            20117..20455
                     /locus_tag="IWK70_000983"
     CDS             20117..20455
                     /locus_tag="IWK70_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9613549.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            20850..21884
                     /locus_tag="IWK70_000984"
     CDS             20850..21884
                     /locus_tag="IWK70_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9613550.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            21877..22563
                     /locus_tag="IWK70_000985"
     CDS             21877..22563
                     /locus_tag="IWK70_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613551.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            22587..23435
                     /locus_tag="IWK70_000986"
     CDS             22587..23435
                     /locus_tag="IWK70_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9613552.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            23632..24405
                     /gene="sufC"
                     /locus_tag="IWK70_000987"
     CDS             23632..24405
                     /gene="sufC"
                     /locus_tag="IWK70_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9613553.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            24418..25704
                     /gene="sufD"
                     /locus_tag="IWK70_000988"
     CDS             24418..25704
                     /gene="sufD"
                     /locus_tag="IWK70_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9613554.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            25701..26936
                     /locus_tag="IWK70_000989"
     CDS             25701..26936
                     /locus_tag="IWK70_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9613555.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            26923..27393
                     /locus_tag="IWK70_000990"
     CDS             26923..27393
                     /locus_tag="IWK70_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9613556.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            27398..28789
                     /gene="sufB"
                     /locus_tag="IWK70_000991"
     CDS             27398..28789
                     /gene="sufB"
                     /locus_tag="IWK70_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9613557.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(28884..30026)
                     /locus_tag="IWK70_000992"
     CDS             complement(28884..30026)
                     /locus_tag="IWK70_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9613558.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(30065..30799)
                     /locus_tag="IWK70_000993"
     CDS             complement(30065..30799)
                     /locus_tag="IWK70_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9613559.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            31031..31306
                     /locus_tag="IWK70_000994"
     CDS             31031..31306
                     /locus_tag="IWK70_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9613560.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            31404..33401
                     /locus_tag="IWK70_000995"
     CDS             31404..33401
                     /locus_tag="IWK70_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613561.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(33477..33812)
                     /locus_tag="IWK70_000996"
     CDS             complement(33477..33812)
                     /locus_tag="IWK70_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9613562.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            33939..34154
                     /locus_tag="IWK70_000997"
     CDS             33939..34154
                     /locus_tag="IWK70_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9613563.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            34191..34553
                     /locus_tag="IWK70_000998"
     CDS             34191..34553
                     /locus_tag="IWK70_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9613564.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            34599..34925
                     /locus_tag="IWK70_000999"
     CDS             34599..34925
                     /locus_tag="IWK70_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613565.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
BASE COUNT        11543 a         6217 c         7345 g         9945 t
ORIGIN      
        1 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
       61 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
      121 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
      181 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
      241 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
      301 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
      361 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
      421 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
      481 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
      541 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
      601 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
      661 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
      721 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
      781 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
      841 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
      901 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
      961 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     1021 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     1081 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     1141 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttggaagcaa tgtatggaga
     1201 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     1261 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     1321 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     1381 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     1441 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     1501 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     1561 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     1621 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     1681 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     1741 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     1801 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     1861 gcaaattagt tgtctttttt atttttaaat ttaacacatg ttgttatttt taatattttg
     1921 ctgatttatt gtttctaagt aaagaaattt aaattgaaac gattgttatt tagggaaaaa
     1981 ggtatttttt gaaaaaagaa tcccgtataa tgattgttgt gcaaacgcta tacaaaatga
     2041 aaaagaaaag agggatgttt atggaaaatg aacagtcggt cgttttaacg aattggaaga
     2101 gaaattattt gttcttttta tcgggacagt ttttgtcagg aatcactagt atggtcgtcc
     2161 aatatgcgat catctggtat ttgacaagag aaaccggttc ggcgacaatt ttgagttttg
     2221 ctactttact aggaatgatt cccatggttt tactaagccc atttgtagga ccgctagtgg
     2281 atcgctggga taaaaaagct cttttgattg tcacggatat tattgtagcg attttcgcgc
     2341 ttattttagc agtagtcgga acgatttcag aatccttccc gatttggcta gtttttgtgt
     2401 ctttgtttat gcgttcggtc gcacaaacat tccaaatgcc aacgatccag tcgattatgc
     2461 cgacaatcgt tccatcttct cacataacaa ggacgaatgg acagttagga atggtccaat
     2521 cagcgaattt catcattgcg ccagctctag gtgcggcttt gttttcagtc gttcctgtca
     2581 attaccttat tttattagat gtattggggg cagtttttgg tgtcggctta ttgatttttg
     2641 tgaagatccc aaaagtttct cctgaaatat tggaagttcc gcttactatt tttaaagatg
     2701 caaaatttgg gctgcagcaa ttgatggata ataaagggtt atggtacata acgatcaatg
     2761 gtgcatttgt gatgctgttg ttcatgccag caatcagtct gtatccattg atgacgctag
     2821 attactttgg tggttccgtt ggacaggcgg gagcagtgga agtagtctac gcagtcggaa
     2881 tgctcttagg cggtgcactg atcagtttta taggaacatg gaaagaccga atgaaaccta
     2941 ttattatagc ctacatcatt atgggcctga cgatcggtgc aagcggattg gttccaaacg
     3001 atagtcaagg gttcttgtac tttcttatct tgaatgcagg agcaggttgt gcaacgccat
     3061 atttcaatac actgctgatg gcaatgatcc agcagagtta tgaatcgaat gttttaggtc
     3121 gtgtcttagg gaacttcaac tctttgatga acctgacagg tccaattgga ttattatttg
     3181 caggaccatt agctgatcgt ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga
     3241 ttttattatg cggaatcgtt ttatttttaa cgtcagctgc ccgaaattat gataaagaat
     3301 tacagaaaaa actggtaaaa gaacaccatg agcaaaaaga cgaatagtat ggaatggagt
     3361 tgtgacagaa gtcttgttac aactccattt ctgatcctaa tgttcctgtg gcaataagcc
     3421 tactcaaatg aaaaatatga gtaattatct ttatttgttt aatacttgat atagggcagt
     3481 tttttcacga cctatcgtac ttcctctttg cttcatagat ttctttgatt tgttgctcag
     3541 ataacggagt gccaaatggt agtttttcct ttaagagttc tctttccggt gcgtctacgc
     3601 ctacattgac atcgttctcg ataggtacgc tgtaatgatg gatatgtcca tgaagattgc
     3661 ggatagtgtc gttaggcccg agcaacatcg gataatgagt caagtaatat tgttgatggt
     3721 cgaatttaac gatggcacct acatcataaa aataaaattt gggacttccg tcagtaagcc
     3781 taggatcatt tttttctaaa taacggaaaa aagcgcgact gtcatggttc cctttgatca
     3841 agtggattgt tccttgcagt tgagtgacca gttccaaaat ttctgcattt gctttttcat
     3901 attgcggatg catagcaagg tcgcctagat gataaacatg gtcggtttct tttattactg
     3961 cattccagct agtgatcaac tgttggttca tttcatccac atttgcaaag agccgcggag
     4021 caaagtcgtt ttttcctaat aagcgttcat ggaaaaagtg catatcactg ataaagtaat
     4081 tcatgtatct tttgcctccc ttttttctta gagtaaacaa aatcacttat ctgagcaact
     4141 cataacatct aaaaagaaaa ggttgtaaat gagacgtgca gctttaagcg ataagaagca
     4201 attttgaaaa atagtttttc acaatttata aaattattcc ttattgccga aaggctggcc
     4261 ctttttatga gtatgccaat tttttatatc gaatgctcaa aagatgagtg attttttggt
     4321 tgactatcat gtttgtgtac tgaataggtc cagtagaaca gtatttttac gacttgataa
     4381 ttaagggatg aaattttcag aaatatgacg tataatgtaa aatactgaaa agttgcgacc
     4441 ttcaactcgt agatgtttca gaatctaatt gaaatgatgg tgatatcatg acagacaaca
     4501 gcaaaacacg cgttgtcgta gggatgagcg gcggtgtcga ctcatctgta acagcacttt
     4561 tgttaaagga acaaggatac gacgtgatcg gaatattcat gaaaaattgg gatgacacgg
     4621 acgaaaacgg tgtatgcacg gccacggaag attataagga tgtagcgaaa gtagctgctc
     4681 aaatcgggat tccttattac tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg
     4741 aatatttcct agccgaatac cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca
     4801 aagaaatcaa attcaaagca ttcttggatt atgcaatgga tcttggcgcc gattatgtag
     4861 ctactggaca ttatgcgcaa gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg
     4921 gagtagacaa taataaagat caaacttact tccttagcca attatcacaa gaacagcttt
     4981 caaaaacgat gttccctcta ggcggcatgg aaaaatcaga agtacgggca attgctgaac
     5041 gcgcaggcct tgcgactgct aagaaaaaag attcaacggg aatctgcttt attggtgaaa
     5101 agaacttcaa acaatttttg agcaactatc tgccagcaaa aaaaggaaat atggtcactc
     5161 ttgatggtga agtcaaagga caacatgctg gcttgatgta ttatacgatt ggtcaacgtc
     5221 aaggactagg gatcggtggc ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag
     5281 atctagcgac aaacacatta tatgtggggc aagggttcca tcatcctgca ttgtatgcca
     5341 caagcttgga tgcaagtgaa atccacttta cgacgaatga accaatgcca aaagaattca
     5401 agtgtacagc gaaattccgt tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg
     5461 atgataatcg tgcagaagtc gtattcgacg aacctgtacg tgcaatcact cctggacaag
     5521 cagttgtatt ttatgatgga atggaatgtc tgggtggtgg tttgatcgac cacgcatatc
     5581 aagaaacaaa agttttacaa tacgtttaag cgatattaaa tgaatcaaaa cagctatggc
     5641 taacaaaaga aacacctttt cttttggata gcatagctgt ttttttgcaa taaaattaat
     5701 ttttattcat tttatacttg aaatacttgt gtaaaaagtt cataatatcc aataatatga
     5761 ataaagttgc tttttgttaa gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt
     5821 gatttgttca attttataag tgagggtgat ggaaatggaa aagaaatcga cgggtattag
     5881 caaatgggga ctggttgctc tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat
     5941 cacaagtgat ctggctagtt ctgcagctcc agggccagcg atcctttcat ggataatcgt
     6001 tgggattggt attttagctt tggtattatc gctgaatcat ttaggagaaa agcgtccaga
     6061 tctagatggt ggtattttcg gttacgcgga agcttcattt ggtaaattag gtggcttcat
     6121 cagtggttgg ggttactggt tgtctgcgtg gctgggaaat gttgcttttg caacgatgct
     6181 gatgagcgcc atgggagaat ttttcccgat attcaaaggt ggacaaaatg ttccttctat
     6241 tttatttgca agtattttta tttggggtct gactttatta gtaaacaatg gagttgaagg
     6301 agcgagcgtc atcaatacaa tcgtgacgat ctgtaaatta gtgccattat tcctattttt
     6361 aatatttgta tttatcgcat ttaaagtgaa tatattcact gctgattttt ggggtaacgt
     6421 atctgataat ctagtgaatg gaacagggca gcaaggaacg atctggttgc agattaaagg
     6481 ttgtttgatg gttatgatgt gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa
     6541 ccgcgcgaag aaacgttcag aagcccagca agcatcgatc attggtctgc tttgtttatt
     6601 gtttatctat atccttgctt cgatcttgcc atacggcgtc ttatctcaag aagagttagc
     6661 aactatttca caacctgcta tggcaaatat tttaaaagga atcgtgggaa attggggagc
     6721 agcgctgatc aatatcggct tgattatttc gatcattggt tcttggttgt cttggacgat
     6781 gctcccagca gaaacaacga tgttgatggc tcgagacggt ctgttgccga aaggttgggg
     6841 aaaaacgaat aaaaaaaatg ccccaactta ttcgttagtt gttactgcaa tcttgactaa
     6901 tctattctta ttgactttct tagttactga ttatgcctat caatttgcct attctttatg
     6961 tacagcggca attttgattt gttatctact tgtcgggatc tatcaagtcc agttttcttt
     7021 aaaagaagga atcaaaaaac aagtagtgat cggtgggatt gctgtagttt ttgaattgat
     7081 gggcatcatt ttagcaggat tttcttatgt ccttttatgt agtattgcct atcttcctgg
     7141 tttttgcttc tactggatcg cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa
     7201 agtaatgatt gggctgattt gtgtggccgc actgctttca atcgggttgc ttgctgtagg
     7261 ctggatcaag atataagggg gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat
     7321 ggctaaataa atgggaaaag aaagcggtat atgatattgc acaaagctcg atcgaagcat
     7381 tgacattaga cgaactcgtt ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac
     7441 gcaaaaacga accattggac tatggctgga tcgaaggatc agatgttttc aaacaagaag
     7501 tcgcttctct ttatcaaacc gtcgatccgg aaaacatctt acaaacgaat ggagcgactg
     7561 gtgctaatct tttagctcta tacgcgttag tggaagcagg agaccatgtt gtttcgatgc
     7621 ttcctactta tcagcaatta tatgacatcc ccaaatcttt aggtgccgct gttgactttg
     7681 ttcatttgaa ggaagaagaa gattggcagt ttgatttaga acagctagaa gccttagtca
     7741 aacctgaaac aaaaatcatc tgtttgaata gtgcaaacaa tcctacaggc acattgctag
     7801 atcggaaaac tttagaaaaa atcgcagaaa ttgctcgtac agtagatgca tatgtattga
     7861 tagatgaagt atatgcgcct ttaacggata aaggtgaatt cttgtcgatc gtagatgtct
     7921 atgaaaaagg tatagcaacc aattccctat ccaaaaccta ctccattcca ggtattcgta
     7981 ttggctggac agctactgga cctgagttgg cagaagtttt tcgaaagtat cgagactaca
     8041 cgatgatttg cggcggagtc ctgtcagatg atcttgctgt acatgcattg aagaataaag
     8101 aaaaaatatt ggcgcgaaac aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat
     8161 gggtagccaa tgaaccgaaa gttgaacttg tcgctcccaa ttacgtctct acttctttca
     8221 ttaaattagc catcgaagaa gatgaccaga cattttgtat caatttatta gaagaaacag
     8281 gagtactatt agtaccagga agtgcctttg atttaccgaa gcatgcccga ctaggttact
     8341 gttgtaaaaa agaaaccttg gaaaagggat tattcctctt atctgagttc ttgaaaaaac
     8401 aggcctaaac aaaaacactg caaggacagt ctgtgacaac tgttcaagca gtgtttttca
     8461 tttacgctta ttgtctatga gaaggagagt gccctgaagc tatggagact cctgaagctt
     8521 tttcttctgc agtttcttgc tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt
     8581 tgaccaaaac aagaagagaa gtcgaaagaa agacgccacg ataaccaaat gcactggaaa
     8641 cactggagcc gatcataggc cctactacgt taccagttgc ttggaaagat tggttataac
     8701 tgaagatccg tccagcagct tgatgaggcg aatattttgt gatcaatgct tggacagcag
     8761 ggagcagaca agcatcagag ataccaatca aaaaacgtaa ggcagctaat tgccaaacat
     8821 tcgtgacgaa tgccattggg atataaacca gaatcgcaaa acccaaacca atcgcaagta
     8881 ttctttcact cccaatcttg tcacctaagc gaccaaatct tggagcggct atcaaagtgg
     8941 caatcccagg tatcgaagca atgattccgc tgatcaacgt aacattccca tgtccatgca
     9001 gcaattggcg aatgtacaga ctgatgattg gactaatgga attgttggat gcttggatga
     9061 tcatcgttgt gacgaacata ccgatgacga cgtgtggata tttcaagtct ttgaaaatct
     9121 gtttggcaga aaccatgttt tcttttttca ctgggacaaa tttttcatgg acgaaaaaca
     9181 gactcaacaa gaagacgaaa aataagatga tccctgtaat gaaaaaggta gggcgatagc
     9241 caaacgctga agcagagatc ccgccaagaa gcggtccaag taatgttcct gtaacagaac
     9301 cagttgctaa ggttccaaga acttgaccac ttttttcttt tggcgtccca gttgcgacaa
     9361 gggcagtggc attactgata taaccagaaa aaatcccttg aagcagacgc aatgcgacta
     9421 attgatacac actagtcacg caacccatca aagaaatgac gattgccatt cctaatgaag
     9481 cacgcaacag catcaatttt cttccttttt ggtcggctaa ttttccccac caaggagaga
     9541 taagcgtagt caccaagaaa gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg
     9601 acgtactata attacctaac gtatctatgt aaagtgacat aaaaggcatg actaaactaa
     9661 atccgatacc agccataaat gttccaaacc ataagacaaa caagttttgt tcccaagttt
     9721 ctcttttcct aaataatttt tcctttattc ttgcagccaa ttagactcct tctttctgaa
     9781 aattttcatc tacagctctt tattataaac tacaatatgt agtttatcaa tgaaagtctc
     9841 ttttttctct ttctttttcg tggtaaactg aaagagtgaa ggatttattt tatgagagga
     9901 aacatggaaa tgaaaaaaag aaatctgtct caagagaaaa tcatcgactg ctttcgagaa
     9961 ctagcagaag agatggatgt tcagcaaata acctttcagc atttagccaa acgcttggat
    10021 atcaaggctc cctcgctgta taaccatttc aaaaatattc gtgatgtcaa aacagcgcta
    10081 acagcgaaac ttctgcagga acttaatgac cagctgcgac gggctcttgt gggaaaaagt
    10141 ggtcaggaag cactgcagat ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa
    10201 gcagtgtatg agttactgat cagtgtaccg cataccaatg aagctgtttt attagatgga
    10261 atccatgaaa cgaatcagat cattttgcag atatttgagg cttttcaatt aacgaaagaa
    10321 gaaaaagttc atcgaagcag ggaacttcgt agtatgatcc acggttatct ttccttacgt
    10381 tttttaggat actttacaaa agaaccaaca atcgatccag aagacagcta tttttggatg
    10441 atcaataact ttatcgctac tttgccttaa acaatcagat tcatttgtaa caattttaag
    10501 atgttcatta aatgaccctt ttataagaga aaattcttgc aactttcttg aaaagtgact
    10561 aagatgagtc tagaaaagct gaaagaagga gcacgagtat gtatcaagta attaccatgt
    10621 tcggggacaa tgagccttgg tggttttttg aagactggga agaagatatt gaagaagaag
    10681 aaacattcga gtctttcgaa gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc
    10741 atcaaaatta tgaatatatc aatgcaaaaa gtaactttct cagcgccttt tggaatgacg
    10801 gagatgagcg ctggtgtgaa gagtgtgacg atgatttaca gcaatacaag ggacttgcgt
    10861 tgttgaaaaa tcatcagccc atcacagtag aaagcagaaa ggaattttat gaaacaacta
    10921 attctagcgg aaaaaccaag cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa
    10981 aagcacaaaa attattatga aggacccaag gtgatcgtta cttgggccct tggtcattta
    11041 ttaggattga aaatgccgga agatctgaat aaggaatggc agagctggca aatggacacg
    11101 ttgccaatga tccctaaaaa actaggaatc aaaccacttc ctaaaacagg acatcaatta
    11161 aaagcaatca agcagctggc tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat
    11221 gccggaagag aaggggaact tgttgctcgc tggatcttag agtatgtgca tttcaataaa
    11281 ccagtgaagc ggttatggat ctcttctcaa acagataaag caatcaaaac tggattcaag
    11341 caactaagac ctgcgaaaga atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa
    11401 gcggactggc tggtaggatt gaatgtgacc agagccttaa ctgtgaagta cgcggataat
    11461 ttatcagcag gccgtgtgca gacaccgaca ctagcattag tgagagacca agaaaagaaa
    11521 atcgagcagt tccgtccaca aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa
    11581 gccaaaatgc ttcaaaaaaa tcaatttgct ttaaaaacac aagaagaagc acaaaaactt
    11641 gttcaattga tcagtacaag caaaggaact gtcacagaag tccaagaaaa attaaaaacg
    11701 gaatctgcgc cattgcctta tgatttaaca gagatccaaa gagaagccaa tcaacgctat
    11761 ggctattctg caaagaaaac attaagtctt gtacaaagtt tgtatgagac ccataagatc
    11821 gttacttatc cgcgtacaga cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa
    11881 gaacgattgc aagcagtcag cgactttgct cctgaggtca agcagtattt gaaaaacgga
    11941 gccatcgtcc aacagacaaa agtattccaa gacagtaaag tgaccgatca ccatgcactt
    12001 ttgccaactg aaaacagagc acgttatgaa aaattaagta atgaagagcg aaaaatctat
    12061 caaatgattg tctctcgttt tttaggactg tttgctcaac cccataaagt cagccaaaca
    12121 aaagttacgg ttgaatttgg taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa
    12181 gcaggatgga aaggcgaaac tgaaactgag acggaaacag tcaaatggga aaaaggaatg
    12241 agaatcaatc ccgatttcgc catcaaaaaa gaactatctg caccgcctaa accattgtca
    12301 gaagcaagct tgcttggtca aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca
    12361 gagattatcg agaaactaat caaatctgaa ctgatggaac gtacccctca aggactgcaa
    12421 gtatctccta aaggtaagca attattagta ttggtaaatc cctctttagt aacaccagat
    12481 ttgacagaaa gctgggaaaa agaccttgaa gctattgctg caggaaagaa acaagcagga
    12541 accttcttga atggaatcga aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc
    12601 aaacaagaat atcaagattt ctcgattacc caaaagaaat gcccagaatg tggggccaat
    12661 ctgcgcgaga aaaacacacg tgacggcaaa atctatgtct gcaccaattc agattgcaac
    12721 taccgcagaa gaaaagatcc aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa
    12781 atggaaatca tcgaaggtaa aaacggtgct tatttccgtt gtaaatacga tggaacgaca
    12841 gaaaaattgc ttgataaaaa agagcagaag aagaaaatga ccaaacatga agaacgacgc
    12901 ttgctgaaaa aatatgcaca agcagaagaa ccagaagaaa gcccattagc agcagcacta
    12961 aaagcagcaa tgaagaagta ataagatatg aaagaggttg tgatagaagt ggtcagcttc
    13021 aagaaataag aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat
    13081 ttccgaagaa gctacttctg gaacaccatt tattcggaaa aaaggactgt gacaagactt
    13141 ttgtcacagt cctttttcat ttaaaaaaat gtttacttca tttgacgtat attattggtg
    13201 aggcttcaat tcatttggct tcactttttc tgtttctctt atgatataaa caggacgctg
    13261 tttcgtttct aagaagattt tcccaatata tttcccgatg attccaagac ataacaattg
    13321 gattccacca atgaacagga agatcgagac catggaaggc catcctgatg taggatcacc
    13381 aaacaacaat gcccgcaaaa taatgaacaa aattgcaata ccagaaccga cacaagacaa
    13441 agcgccgata aatgaagcaa tcgttaacgg tgcatccgag aagttcacga taccatcaat
    13501 cgagtagttg aataatttcc agaaggacca agatgtttcg ccagccacac gttcgcggtt
    13561 ttcataagaa atatattctg ttttaaatcc gacccagcta aataatcctt ttgaaaatcg
    13621 gttgtattct gttaattcta ggacggcatc caccatttga cgagtcatca agcggaaatc
    13681 acgagcgcca tcgaccattt ctgtatcgct gattttattg atcaactggt agaatttttt
    13741 cgcaaagaaa ctgcgtatcg caggctcgcc ttcacgagtc gaacgccggg tgccgacaca
    13801 atcgatatca gaagtagtga tccggttgat catctcagga agcagttcgg gcggatcttg
    13861 cagatcaaca tccatcacgg tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag
    13921 agccgcttct ttcccaaaat ttctggaaaa tgagacaaag cggacataag gataagctga
    13981 ggctagcttc ttgattgctg ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata
    14041 ttcgaactca tgggcacctt tgaatttttc aacctcatca aaaaaaagag gaacagtttt
    14101 ttcttcatta aaacatggga caacaattga tactttcatc gtttctatcc tttcaatcgt
    14161 atgtttatat atggtttaca ttcaaaattt aataaacctt aaataacacc tttaaaattt
    14221 gattttacgt aaaagaacag gtattctttt tgtaaactaa cgtatttagt ttaccatgtt
    14281 tcctcggttt atcaaacttt taagaacatt ttttattagt tggcaagtag ttttggatag
    14341 agaaaaagga ggaagttcca gtctattgac taggagcaaa caaaatgaaa atacacacac
    14401 ctatcggctt tttatggtta gatgcagatg aatccggatt gactgctgta tcttttcagc
    14461 caatttcaga aagcgaatca gcgaatcagc gaattttaac gcgcacacaa gaacagtttg
    14521 aagaatattt tgcgggaaaa agaaaagaat ttgatgttcc tttatctata aagagaggta
    14581 cgccttttca gcaaaatgtc tggaaagctt tgtcgaccat tccttatgga gaaatccgaa
    14641 cgtataaaga agtagccgtt gctgttgata gtccgaaagc tgtgcgagcg attgggcagg
    14701 caaatcgaac caatccattg ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg
    14761 gaaaactgac aggatatatg ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt
    14821 tgttgttaga aggatatctg caaaattaaa aacagttgtg attcggacat tgttccatgc
    14881 gagaatcaca actgtttttt catgttcatt ctaaactttg atcagccaaa taaatagtaa
    14941 agccatcact gtttggagaa gcccgacagc taatccataa gataagaagc gtgtaccttc
    15001 tttcatgaat tttgcaaagt ctaaacgcag accaattgcc gctaaagcag tgatctcaaa
    15061 ccagctgctg ataaaatgtg ccgattggtc aataactaca ggtaaagaaa acaagctgtt
    15121 gaccaaacaa aagatcacaa accctaatac ataccaagga agcggtaatt ttcgtttttt
    15181 ctgcgtatca gttggcgttt caaacagagc tggttgtttc ttgtgcatga atttttcaaa
    15241 cagatagaca acaacaacga gcatcataat acgtagaatc ttaaatagca tggcgaattc
    15301 gacaacagaa ttgtcaagta agcttgcccc agcgactact tgaccaacag attggagtgt
    15361 accacccaaa agtgcacttt tttcaatgag atttccacca aacaactgta atccaagaaa
    15421 agggagagtc aacatcatca cagtacccag tagattgact agtgtgatga tctgtccttt
    15481 ttcttcctct ttcgcatgga tggaaggggc gattgaagca atagcagaag aaccacaaac
    15541 agcgtttcct ccagccatca tcaaagaaga agtatcgctg aattgcagtt ttttaccaat
    15601 caagtaagca ccgatgatag tgactgacat ttgcagtaac acaaaaacaa ttccttttac
    15661 accaatttga gcaatcgttt gagctgtgac agttgcacct agtaaaaaaa cagagaattc
    15721 taatagtttg ctttcagcaa attttgtgcc gattgctaga acaggctgtt taaaaaaagt
    15781 atttcctaat aaaataccca ataaaatagc aatagtcgct ccacctaact gtggcagata
    15841 aatagcaaga agcctgctga aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat
    15901 ttcgcggacc gctgaatcct ttacagattt tattggatgt gacaatgatt tcatttgatc
    15961 ggttcctttc gtcaataaat actaaaagca tactcgaaca tagtagcact aaaccagtat
    16021 tttgacaaaa gaacaagagg tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa
    16081 cggtgttcca gaagtagctt ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc
    16141 aattttcgta aattccttct tatttcttga agctgaccac ttcttttacg acctcttggt
    16201 aaacggtgtt caaaagcttg catctgcggt aataagccca accagtacaa aaacatgaac
    16261 agctgttttt gtttggccgt tcttattact tgatgcagaa cagctttttc acaacctatt
    16321 gaatcgatta ttaagcgatt tttttagaaa caaattttcc atgtttgcta tgttctttaa
    16381 ttaagaaacg tacgtaatga gcaagaacgt agaagacaac tacacttgca aacagacatt
    16441 ccgcaacttt taagaaatcc aatacgctac tatagatacc taaactgttg aagtaatgaa
    16501 cagtagtaaa tatagctgtt gcagagataa caagcgggaa tgtaaaggct gcatagcttg
    16561 gataaaaacg aagcttgaac aaacgtggaa gcatagacag cacaacgaaa tacaatactt
    16621 gagataaagc gaacaatgct acgacaagat aaaaattagg ttcagcaaag tttttaagat
    16681 aacctgttag gcaaagagaa ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat
    16741 gagccatatt tcttacgaaa aagacacgtt tgatgatgat cggcaataag aacgcatagc
    16801 agatcaatga aatccaaaag aaaatttttc caataagtgg gaagaagttc ccggcagtta
    16861 cagtgatgac cccaaatcca acgtaaatga taaaccagct tggataaatt gcttcaattg
    16921 aaagatcagc tttgataaca tggtgataat tgaaataaaa gacaaggatc acttggaaga
    16981 taacagcgca cagccaaatg aatttgacaa ttggtgcaat actttcatat gaaacaaaat
    17041 aagtacaaat aaccatgagt gacatcgtaa aagttggtga aacggaggcg atgattggat
    17101 catgcaacgt ttgtttcgtg tgttcgaata agataaacaa tttaacaaaa atgaatatca
    17161 taagaatcat agagataccg ccagttagat tgcctaaagc aacatggtga tagtctttaa
    17221 acagatttcc aagggaagct aacccaagga tcaacccgca gatcgggatc ggaataattt
    17281 tcaaaaagtt cttcatatat aatattctcc tttttattta atagatttta gtaccaagat
    17341 gaacgaatac attgtaaaat gaataggaat gtatgattac gagcaagcag acagctgatt
    17401 gtcttcatct aagttactag ttggatcagc aactgttttg ttgtacattt ctattataga
    17461 tggttttttg tatatttaaa tcaaatagtt tttatagtaa ttatcataaa aagttatgga
    17521 agtggtgcca tgtttaagtt attaacgact tttcgagcag tatatgaaac aagaaatttt
    17581 tccagagcag cagaagtact gtttttatct caaccagcag tctcgaatca aatcaaacag
    17641 ttggagcggg aattgaatat cgatcttttt caacgtaacg gaagaaaaga gatgcttcca
    17701 actaagcaag ccgacttatt atatgaacgg acactggttt tgttagaaga atgggaagaa
    17761 actcagcaaa aattgctcaa tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc
    17821 cacacctttg ctgtctatct tttgcctcag ttgatgaaac atctgatcaa aaggtttcct
    17881 aaagtgcatt ttgagatcga aatcacgaat tctcatgaat cattagaaca agtagcgaaa
    17941 catgaatccg attttggttt tatcgaaaag ccattagaga cttccggcgt acaaagatac
    18001 tctttgatgg aagaccagct agtcatcgct ggggacccaa aaagcaaatt gtggcttgta
    18061 agagaagcaa actctggtgt ttttcattac acgcagcgtt attttgaaga gatgaatatc
    18121 caagataaga agatgatcgt gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga
    18181 atcggtcgct cgattttgtc taagcgctct gttccagaaa atgtgccaat gactccgttg
    18241 aatttccatc gatattttta tttcatccaa cgaaccaatc tggtttctga tcagctgcta
    18301 gagattgccg aagagatcct tcgatacaat gaaagcacag aaacttctca cagcgaggag
    18361 aatctcagct ggtagataat tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa
    18421 gcagtaggct tttgtactgc atttgctcaa gcaactgcac aaacggaaag ttaatttttc
    18481 ccaaatgtct atggaaagac tttccattat ctaactggac cgctataata atcagcagta
    18541 tggtttttaa gaaaagggat gaaacatgga aaaaaaatta aaagcttcga acgataatcg
    18601 tattgattat ggcgtgattt taccagtctt tctgttatgt ttgattggtc tgctctcatt
    18661 atatgtagca cttagccatg atccgaacaa tccagcagtc ttgaaaggcg tagggatgca
    18721 agttgtctgg tatttagttg gagcggcagc aatcgttgtc atcatgcatt tgaattccaa
    18781 atggatctgg aaactgactc cttaccttta tggtttgggg ttagtggtaa tgggactctt
    18841 gctcaaattc tatgatcaga atttagctgc ttcaacaggg tctaaaaact ggtttcgatt
    18901 cggtgcgttt actttccagc cagcagagtt gatgaagatt gcgtacatct tgatgatggc
    18961 actgatcgta actaaacata atacgcaagt aaaagaacgg acgatgaaat cggacttttg
    19021 gctgattggg aagctcttga tcgtgacaat accggtttta gcattgatca tggcccaaga
    19081 tgactttgga acgatgcttg tctttcttgc gatctttggc gggattttcc tgatgtctgg
    19141 aatctcttgg cgcatcatcg cacctgtagt aatcttggca gtagtcgttg gagcaggaac
    19201 gatcttcttg gttacgactg aaggcggaag agacttgctt tataaagttg gattcaaatc
    19261 ttatcagttt gcacgtattg attcatggct ggaccctttc catgatacgt caggaatgag
    19321 ttatcagccg gcccaaggac ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt
    19381 caacgtaagc aatatctatg tgcctgttcg tgagtccgat atgattttta ccgttatcgg
    19441 tgaaaacttc ggctttatcg gtggtgcatt cgtcattttc ttgtatttta ttttaatcta
    19501 tcgtatgatt cgggtgtgtt tcgatacgaa taatgaattt tacgcctata tcgcaagcgg
    19561 tttgatcatg atgctgctgt tccatgtctt tgagaatatt ggagcaaata tcggattact
    19621 tcctttaaca gggatcccat tgcctttcat tagtcaggga ggatcctcga ttttagggaa
    19681 catgatcggt atcgggttga tattatcgat gagataccaa aatgaagcac ctgcaagatc
    19741 ttcaaggagg taagaaaatt gtacaccttt tactggtacc cgaaatgttc aacatgcaaa
    19801 aaagccaaag cgtggttaga ccagcatggc gttagttatc aaaccgttga tatgatcaaa
    19861 gaagtaccga aggcctcaac gattgagact tggctagaaa cgagtcaagt accaatccgt
    19921 cgcctgttca acacaagcgg gatgaaatat cgggaacttg gactgaaaga tcaagtaggc
    19981 gagtttacgg cttcaaaagc gagccaagtc ttatcgacag atggtatgct gatcaagcga
    20041 ccactgatct tacgagagga ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa
    20101 ggagaactga tataaaatgg caggcaagca tttgaagaaa aaggacaacc tttggatctt
    20161 atttaatgga aaagaatact gtgttggttt gacaaatgaa gcgcaagaag aacttggaga
    20221 catcacgttt gccaatctgc caaaagcagg acaatcgcta aaaaaagatg aacctctgat
    20281 cgaagttgaa gcggaaaaag cggtcaatga atttgccagc ccgctaacgg gtacagtttc
    20341 ttctgttaat gataaaatcg gtgaagatat caatatcttg aatgattcag atgaaatgaa
    20401 tgcgtggatc ttaagtttca aagatgttga tccaaaagaa tttgacgaat tataaaatca
    20461 gaaaatcact tgaaaaaagt tgacactgat tcttccagtt gctataatca taagcaatcc
    20521 tatatgaaac gagaaagctt tgaaaagaag agtacatgtc acggacgttt gaagagagcc
    20581 ctgtttgctg aaaatgggta acgaaaatga gatggaagat ggtcttagag cagagtaagc
    20641 gagcgtaagc aaacttacta cgggagtgcc cgttacagcg cgacagtata cggaaagaag
    20701 ctaagtccaa gtactggaag aggctgttat tgcgaagtaa cggtaaatca aggtggtaac
    20761 acggaagttc aagctttcgt ccttttgctt aaggcaagag ggcgaaggct tttttgtttg
    20821 aaaaatcata agagaaagat gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa
    20881 agacattcaa tgggaaaaac ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg
    20941 aaaaagggga tatttatgga atcgtcggct attctggcgc aggaaaaagt acattagtcc
    21001 gtctcctgaa tggacttgag cttcccacga gcggcgaagt gatcgtaaac gatcaagata
    21061 ttacaaaatt aaaaaacaaa gaattacgta cattccgaaa gaaaatcggt atgatcttcc
    21121 agcatttcaa cctgttatgg tcaagaactg tacttgaaaa tatccagctg ccgctggaat
    21181 tagctggcgt accaaagagc aaacgtaaag aacgggcaga agaactgttg cgtttagtcg
    21241 ggttagaagg acgcggacaa gcatatcctt cacaactttc cggcggacag aaacaacgcg
    21301 tcggtatcgc acgagcattg gcaaatgatc ctgagatcct gctttgtgat gaggctacta
    21361 gcgcattgga cccgcaaaca acagaagaag tattagactt attgttagcg atcaataaga
    21421 aattgaattt gaccatcgtc ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca
    21481 acaaagtcgc tgtgatggag ttggggaaag tcgtagaaga aggagacgta ttgactgtct
    21541 tcagacaacc gaaaaaagaa gtaaccaaac gctttgtaca acaagacatc gaaccagaag
    21601 ctgattcgac agaactatta gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa
    21661 ctttgacctt tagcgaaaga aatgccaacg agccagtcat ttcccaagcg attcgacgct
    21721 ataacatcga tgtcaacgtt gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg
    21781 gttcattgac tgtcttgatg actggtgaaa ccaaagaatt agatcaagca gaagtgttca
    21841 tcaaagaaca aggagtagga attgaggtga tccatcgtgg ataaaagctt ttcagaaaca
    21901 tatctagatt ttaaacaaat caacccggaa acgatgaatc aggctatcct tgatacacta
    21961 tatatgacat taatctcaat ggtcttagtg acgatcatcg gattagtttt gggattgatc
    22021 ctttactcta ttggacgcaa aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg
    22081 atcatcagta atattttccg gtcaacgcca tttatgatcc tgatggtttt gatcattcca
    22141 tttactaaag caatcgtagg cactatgcta ggtgcgcaag cagccattcc agctcttgtt
    22201 ttatccgcag caccatttta tgcccgtcta gtagagattg cttttcgtga agtcagccct
    22261 ggtgtactgg aagcagcaga tgcaatggga gcaagttact gggaaatcat ctggaaggtc
    22321 ttgatacctg aaagtattcc cgcattgatt tctggattaa cagttacaac gatctcgatg
    22381 atcggattta ctgcgatggc cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg
    22441 caggaaggct accagcgtgg taatctaaca gtgacattcg ttgcgacttt aattatttta
    22501 ctaatcgtat ttatcgttca aggaatcggt gatttcttaa ctaagaaaac agacaaaaga
    22561 taataggata tagggggatg taggatatga aaaagaaaat ttttggattt gcagcagcgc
    22621 ttttgctgac tgtaggatta gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa
    22681 ataaagcaga cgacacgaca ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt
    22741 tggaacatgt gaaaccatta ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg
    22801 atgactatgt attaccgaac caatctttgg aagaaggcga cattgatgcc aattacttcc
    22861 agcatattcc ttatttgaac aaagaaatca aagaaaaagg atatgacttt gtcaatgccg
    22921 gtgctgtcca catcgaacca atgggcttgt actctaaaaa agtcaaagat gtttccgaat
    22981 taaaagatgg tgcaacaatc attacttcaa attctgaatc agactgggga cgcatcatca
    23041 caattctaca agatgctggc ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa
    23101 cattcgatga tattgaagaa aaccctaaga acttgaagtt caaccatacg atcgacccat
    23161 cacttttagc ttctacgtac caaaatgatg aaggtgacct agttgcaatc aacgcaaact
    23221 tcgcttacgg cgcagggttg aatccattag aagatgccgt attgcttgaa aaggacaact
    23281 ctccttacgt gaatattatt gcgactcgta aaggtgatga agacagtgac aaaatcaaaa
    23341 aactgattga agtgcttcac cgcaaagaag tccaagaatg gatcacagac aaatggggcg
    23401 gctctgttaa accagtaaag gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa
    23461 taagtctgta atggcttatt tctcatcttt tttataccta ttctaattta gaaatcaaaa
    23521 ttgtttaata ataaataaaa gatatttgat tttagtaagg aaggtcattg cgcttcattc
    23581 tcaattagtc taaaatgagt gagaacgaaa gagaatttgg gggtataatg catgtctgtc
    23641 ttagaaataa aaaatctaca tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac
    23701 ctaacaatga aaaccggaga aatccatgcc atcatgggac ctaacggcac aggaaaatct
    23761 accttatctg ccgctatcat gggcaatcca aactatgaag taacagaagg cgaaattttg
    23821 tttgacggga aaaatgtttt agacttggaa gtagatgaac gtgcgcgact tggcttattt
    23881 ttagctatgc aatatccaag tgagatacca ggtatcacga atgcggaatt catgcgtgca
    23941 gcgatcaatg cgaaacgtga tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag
    24001 ttagatgaaa aaatggcttt actaaatatg ccagaagaaa tggctgaacg ttatctgaac
    24061 gaaggttttt ctggcggaga aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa
    24121 cctacatttg caattttaga cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc
    24181 gttgctaaag gagtaaatga aatgcgcgga gacaatttcg gtgcattgat catcacccac
    24241 tatcaacgat tattgaacta tatcacacct gatgtcgttc acatcatgat ggaaggccga
    24301 gtagtcatga cagggaatgc tgacctagct aaacgtctgg aagcagaagg ctacgctggt
    24361 atcagtaaag aattaggaat cgactataaa gaagaagaag cgtaaggggg agcaagaatg
    24421 aaagaaaaaa actggctcga ttatcttgac gcggtaaatg atttttcttt aagtaaaggc
    24481 gaacctgatt ggatgagaac cttccgtcaa gacgcactag cgaaagcaga tgaactgcca
    24541 ttgccgcata tcgatcgggt gaaattccat cgctggtcgt tatttgatgt gaaagaaaca
    24601 caaaccatca gtgaaacagg cacgattccg gcatttgatg caatgaaaga caatcctgtg
    24661 cttgtccaac aaggttcatg gacgattttt gaacaactgc ctgtggaatt agcagaaaaa
    24721 ggagtcattt ttaccgactt gttcactgca atgatcgaat atccagaact agtccaagaa
    24781 tattatatga aaaaagcagt aaacatgaac gaagaccaat tgactgcact ccatgtagct
    24841 tttatgaaca gcggtatttt cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc
    24901 gagtccttat tcattcaaga tggcgcatcc gatgagcact tcttcaagca tgtgttgatc
    24961 gtggcagatg aacatagtga attttcttat ctggaacgtt tccagacaac caaagaacaa
    25021 gtagcaaaat catcaggtaa tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc
    25081 aaatattcag ccgtagatca attaggagaa aatattactt cttatatgaa tcgccgtggg
    25141 cacattttac gagatgcttc tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg
    25201 atcgctgact ttgactcaga tttagcagga gaaggtgcac acgcagaagt caaaatcgtg
    25261 gcaatcagct cgggcagaca aatccaagga atcgatacac gagtaaccaa taaagcccct
    25321 catacgatcg ggcatatttt gcaacacgga gtgatccgag aaaaaggaac attgaccttt
    25381 aatggtatcg gccacatctt gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt
    25441 gtcttgatgc tttctgataa agcacgtggt gatgcgaatc ctattctttt gatcgatgaa
    25501 aacgaagtaa ctgctggaca tgctgccagt gtaggacgag ttgatccaga agaaatgtac
    25561 tatctgatga gtagaggatt gcataaagaa gaagcagagc gcttagtgat tcgaggattc
    25621 ctcggttctg tcttaacagc aatccctgta gaacaagttc ggaaagaatt agtcgaagta
    25681 atcgaaggga agttgaatgg atgatcgatg ctaaaaagct acgcaatgat tttcctatct
    25741 tatttcaaga cgtcaatgat gaacctttag tttatctgga taatgcagcg acgacccaaa
    25801 agccaaaaca agtactccag gcattagcgt actattacga acacgataac gccaatgttc
    25861 atcgcggtgt ccatacgtta gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa
    25921 aagtacgggc gttcatccat gcaaaagaaa cagcagaagt gctgtttact cgtggtacga
    25981 cgacaagctt gaattgggta gccagaacct tcggcgaccg ctatatcaaa gcaggagacg
    26041 agatagtgct ctcttacatg gaacatcatt ctaatgttat cccgtggcag cagttggcaa
    26101 aacgaacagg tgctgtcttg aaatatatcg aactaacaga agaaggcttc ctggatatgg
    26161 aggatgcagc gaaaaagatt acggacaaaa ccaaaatcgt ctccattgct catgtatcca
    26221 atgtcttagg tgtcttcaat cctgttcaag aattagctga acttgtccat gaaaacggcg
    26281 gggtcatcgt agtagatggt gctcaatctg ccccgcacat ggccattgat gtccaagcac
    26341 tagactgtga tttctttgct ttcagcggac acaaaatgtg cggaccaaca ggtattggtg
    26401 ttttatatgg taaacgaaaa tggttagaag aaatggaacc tgttgaattt ggtggcgaaa
    26461 tgatcgattt tgtcgaatta tacgacagta catggaaaga actaccttgg aaattcgaag
    26521 cagggacacc aaatatcgcc ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa
    26581 aactcggtat ggacaatatc catcgatatg aagaagaact agccgcttat gtgttgccaa
    26641 aattacaagc aattgatgga ttgactgttt atggtccaca agacccgaat tttcggacag
    26701 gagtcgttgc attcaatttg gatgggttac atccccatga tgtcgcgaca gctttagaca
    26761 tggaaggggt agccgtcaga gcaggtcatc attgtgcgca accgctattg aagtaccttg
    26821 aagtatcagc gactgctcgc gcgagctttt atttctacaa cacaacgcaa gacgccgacc
    26881 gtttagttga agcaatcatg gcaacaaagg agtttttcca acatgggact atctaaatta
    26941 gacaatttat accgtcaagt gatcttggat cattcttccc atccgcatca tcgtggaagc
    27001 ttgactgact cgacaagcaa gatcgaattg aacaacccca catgtggaga tgtgatcgaa
    27061 ttgcaagtcg atgtaaaaga cggaatcatc aaaaatatcg cctttgacgg cagtggctgc
    27121 tccatcagta cggcaagtgc ctcgatgatg acagacgccg tcatcggaaa atcaatagaa
    27181 gaagcagaac atttaactgt agactttcta cagcttgtcc aaggaaaaga agtcaaagat
    27241 cccgaccaat taggcgatgc tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc
    27301 aaatgtgcga cacttgcctg gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca
    27361 gctttccatc attaccacaa ggaagaggag tgagaacatg ggcgtaccag aattagaaga
    27421 atataaattc gggtttcacg atgatgtcga accggttttc agtacaggag aaggcttgac
    27481 agaagaagtg atccgtgaga tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt
    27541 ccgtctgaaa tctctagaaa catttaacaa aatgccaatg caaacatggg gccccgacct
    27601 ttcagacatc gatttcgatg ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg
    27661 tgactgggaa gacgtaccag aaaaaatcaa agatacattt gaaaagattg gtataccaga
    27721 agctgaacga gcttatctag ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca
    27781 taatatgaaa gatgaattcg aaaagttagg aatcgtcttt actgacactg actctgcttt
    27841 gaaagaatac cctgaattat tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa
    27901 caaattagca gcgctgaatt cagccgtatg gtcaggtggg acctttattt acgtgccaaa
    27961 aggcgtcaaa gtcgatgttc cattacaaac gtacttccgg atcaatgcgg aaaacacggg
    28021 gcaattcgaa cgtacgctga tcattgttga cgaaggagca agcatccact atgtcgaagg
    28081 ttgtacggca cctacttatt caagtaacag cctgcatgcc gccatcgtag aaatctttac
    28141 aagaaaagat gcgtattgcc gttacacaac gatccaaaac tggtcagaca atgtgtacaa
    28201 cttagtaacc aaacgagcaa aagcatacga aggcgcaacc gttgaatgga tcgacggaaa
    28261 cctaggtgca aaaacaacga tgaaataccc aagtgtttac ttagatggaa aaggtgcacg
    28321 aggcaccatg ctctccatcg cctttgcagg agcaaaccaa atccaagata cgggtgcaaa
    28381 aatgatccac aatgcaccaa atacatctag ctctatcgtc tctaaatcaa tttcaaaaga
    28441 cggcggagaa gtaaactatc gaggacaagt aacctttgcc aaaaacagtg cgggttcgat
    28501 ctctcatatc gaatgtgaca cgatcatcat ggacgaatgg tcgaaatccg atacgatccc
    28561 attcaatgag atccacaaca gccaagtctc tttagaacac gaagccaaag tctcaaaaat
    28621 ctcagaagaa caactctact atctgatgag ccgaggcctg accgaatctg aagcaactga
    28681 aatgatcgtc atgggctttg ttgaaccatt caccaaagaa cttccaatgg aatatgcagt
    28741 cgaactcaat cgattgatca gttatgagat ggaaggcagt gttgggtaaa cctgttatag
    28801 gagctagaac ccttgatata taagggttct agcttttttg ttttgcctgt atactagaaa
    28861 tgaccagatt ttgaccagct cttttactga tgagccatca tatcagcaaa gtcttgacct
    28921 gttttcttag gtgcattatt tgtaatatgt gcatagatgt ctaatgtcac tttactattt
    28981 tcatgcccta atcgttgttg aactaccttg acagattctc ctgcttctaa taataaacta
    29041 gcatgtgtat gtctaaaccc atgaggtgta atacgaggca agttatattt tttcaaaata
    29101 tatcttaacc aatcattaac agtgtttgga atgtatagag tgtttttaac tgttgtaaaa
    29161 acatgttgct ctttagagct agtattgtat ccaagtttta aatactcttc tttttgctgt
    29221 aatttccagt tgttgaggat agaaagagtc tcagtatcta aagagataac tcgtctagaa
    29281 gctcttgttt tcggagattg aattataact ttaccaaatt catctacagc aagagtttta
    29341 ttgacatgta gttctttatt aaacgtgtct atgtctttcc actgtaaagc gagtacttca
    29401 ctctttctca tacctgtgta tgccaataat ctgaagaagg cgtattgttt catgttacca
    29461 aaatctttaa aagcatccag taaattatta agctcctctt ttgagtaaaa tttacgattt
    29521 ttctcatact ctttttttcg tggaagaagt gttttttcca taggattatt gtcaatatat
    29581 tgcattgcaa ccccgtagcg taaaatttgt gcagtctgtt ttctcatata atcgtaactt
    29641 tcatatttac tgtgccattc atctaccact ttttgacaat atgggacaga gatattcgat
    29701 agtttaagct taccaaatgc tggaagtatt tgattatttg cgtatcttac tgagattgcg
    29761 attgttgaag ctttaacact ggtacgctga tgagttatcc attcattata caggtcttca
    29821 aatgtcatat caggtgcttt ctttttaaca ccgtataata tttgatgtct gtactcgtca
    29881 taatcttttt gagcttcttg tttgctacta aaacctcttc tcgtagtatt tattttttca
    29941 ccagttcgtg gatcggtggc aatgtgtcct ttatacatcc acgccacttg accgtttttc
    30001 tttttgtact ttgatatttt tgccattaaa tcatctcctc ttagtttaca aggagtactt
    30061 accactagat ggtatcacct cccttcattt ttttcttagt gtattttttc ccaattgatg
    30121 aaatcgtttg ttctagcttg tttatattat cagtaatttc tctcacagct tcttcgggat
    30181 aagacatgtc tttcaattga ataggtagcc attcatctac cattctttct agtagttctt
    30241 ctatactgct agtgaggaaa agaatgttat tgtcaaattc ttctacaata gcattgatga
    30301 ataattcttc tatttttaag atattgaaaa gactaatttt ttcattttta actttattac
    30361 aagtcttgtt agcaataaat tcttgcatct ctggttctaa tttggaatat ctatccagcc
    30421 atgtatctaa attagagtgt actggtggga actgttgacg aagatgctgg aacaggtttt
    30481 tatctggaaa tgttatacca tctagtacaa tgctatctct ataaagaata attcccaaaa
    30541 tatattcttc tacattgcca caaactaact cctctatact catatttccc aaatcagcaa
    30601 ttcgggataa tcgctctggt attggtaaat aggttccctt ctcccattcg tagatagttt
    30661 ttttgtcaat taattgaccg ttgttaatca taagaccaaa tgctggttgt gttaattttt
    30721 tactctttct tatagctttt atttttgttc cgagtatctt ttttcgttct aaataattta
    30781 ttggtggacg ttttatcata ttgaccacac ctcctaaaat tagtatacct caaaaaaggg
    30841 gtgttgcaaa taaattttac aaagctgttg acaccccttt tttatcgagg tattatgtgt
    30901 atgtcgatga actggtaatc attttttgtt tgattaatcg gttaataaca aaatactttt
    30961 attttgagca aaaaatacga agataattct tagaaaattt gttcaagcaa tcacgaaagg
    31021 agggggaaat atgtctctac aaatcattga tagcattgtt tctatacaag ttgaacaaca
    31081 agtgaaagag ataattacag tgctaaagcc tatgctagaa gacattattt ctggagtcac
    31141 tctagagtat atggactatc agcaggcaag tgaatatcta ggtgtatcag taggaacgat
    31201 tagaaaatat gtttctcaat acgggttacc tgttataaaa attgatacgg tcattagatt
    31261 taaaaaatct gatattgata gtttcatgga acggtataaa ggttgagtaa acaaataaga
    31321 ctaaaacata tagtaataaa gagtttgttt ttattatctg tatgttttag tctttaacag
    31381 aattttataa ggaggaaaca caaatggaaa catcaatgat taatattcca atttatgtag
    31441 atcaagaaaa attattagag aagccagtta caaaaaaaga aaaaactgat gtaagagata
    31501 ggtgtttaga taactataga gaagttacaa gagatgagtt tgtagagata ctaaattcat
    31561 cacaatcatt tattccaagc aagacaaaga ataaaggcaa tgacaaggag agttttgtgg
    31621 aaaccagagt aattattctg gatgtcgata acactgttaa agatgagaag gggaaggtag
    31681 tagatttgag taaagatgat agtagatact taagcgtaga aaaggtttta tctatcaatt
    31741 tagttcataa ttctgcgttt gcgatacaaa aaagtattcg gtactctgaa aatctagaaa
    31801 aatacaagat tgtctttcta ttaaaagaag caattactga ctatggtaaa atggttgctg
    31861 tttacgaata cttgaaaaag aacatccccg ggtgtgatga caatgtaaat gcttcgaata
    31921 gaatgttttt tggaggatat aagtcagatg cagtgataat aattaatgaa gacaacatgt
    31981 tagatattac tgagcttccg attgatttta ccatgacatg ttctaaaaat tgtcaaagtg
    32041 attcagggag agagtatgaa ctagtcaatg aaaccgattt tgttaagttg ataaaaaatg
    32101 acgacaaaga agaaatgaaa cagtggttta aagactgcct gtttgattcc tccgacatgt
    32161 cttttaatga gatttatgaa agattgttaa ccattgatat gaataaatta cttaagtcaa
    32221 gtaaaaactt aagatgtctg tttcacaacg atcataatcc ctctgctagt atttttacca
    32281 gtaaaactgg gcattccatt ttttattgtc attccagtaa ttgtgatatc agtgaaaatt
    32341 ttattggagt tgtgatgcta attttaaaga aagacagtcg ggtagaaaca tttaattggc
    32401 tattagagaa cctagacttg tatcctacac attttcgaaa attgaaagag gaatcaaaag
    32461 tactttttga cactcttgaa tcaatgaaag acaaactttt caggacaata aggaattatc
    32521 tagaggaaat gcgacgtgtc tatgatgtct tgctttctga tgttaatttt tttgatgata
    32581 gtaaggaaag tgtgacttgt attttgtcgg gggaacaatt agctaaaaaa atgagttctt
    32641 actatggtaa aacttatgac atagataaat gtaataagtt actatcactt atgactttta
    32701 taggattgat aaaaaaatta gatgatgaag atgttccttt gaagatgctc gaatacctaa
    32761 ataggttaaa ggaagagaaa gcgttaggaa actttaatca taagagaagc aatgtctatc
    32821 gattagacgt ttttaatgca acttctgtga ttcaaaaatt gaatgaagat atccttccac
    32881 ttctggaaaa aattcatttt acatatcaat atttttctta tgaatgggtt aagatatgtt
    32941 ttaacgaaaa agaggctaaa agagtatttc ctcaaaattc ccgatcagaa ctcagcaagg
    33001 aaaaacaatt gattttagat gaagcacatg aagttttaca gggcttatcg tggcaaggga
    33061 catatctaat gacagagaaa gaacttatta acactgtttg tgaacgccta cgtaatgttg
    33121 gggataacag cgtgaaaaat gctttaataa aaaacagagg ctactttgtt aaacaaggat
    33181 tcactcttga acgagcttct aaggatatta agaaactata caatgtgaaa gcaagtagta
    33241 gttttttaat atatttagcg aaaggggcag ggatagatga aacaataaaa gaattgatat
    33301 tatcaggagc tgggatagtt cctctgtatc aatttacaag tgtaacaaga gatgatggac
    33361 ggagagtaag gaagagaaaa gacccaattg tattaaacta gacaggttat ccatagctct
    33421 taatcttatt tcatctcaat acacccaccg ttaaagttcg gtgggttatt tatttttcaa
    33481 tctttcaata gctgattgcg ttcattttct tgccttttta atagcttcat atagtcaatt
    33541 gttcgaactg catcttcagt gctaagagaa cgatagagag ccaaaaactc ttcctcataa
    33601 tctacgacaa cattgtctag aaaatggctt acaggggttc ctagggctct agaaagagct
    33661 gttaagctat cgaggttagg ctctgttaca gaattttccc atcttgctac tgcctgttgt
    33721 gtagagaaga ttttttcagc taattctttt tgtgtccacc cgagagcttt tcgcttttct
    33781 ctaatgacct ttgcatattg tttctgtttc attaatacac ccccatctca gtatagtaca
    33841 acaagttgtt gtatttgtaa tggtatttga agttttttat attttatgag ttgaaataca
    33901 tcatattgtt gtatataata aaacaggagg ggagaatcat gaaaaaagta agtaatctca
    33961 gccgatacag aaaagaaaaa ggactttctc aaacggagtt agcaaaaaaa atgaatgtta
    34021 cacagcaatg tgtaagttcg tggcaaacag ggagaacgat tcctaaaccc tatcaaatga
    34081 agatgctttc tgagatatta agtgtaccaa tgaatgagct gttttctgat gtatttaata
    34141 aagtaaacag ttaactaata agcaattgaa tgattttagg aggagaagac atgtattgta
    34201 gaaaatgtgg caaagaaata acagatgaaa gtaagtattg ccagttttgt ggagcactac
    34261 aagaaacaga tgagacaacc ggaacagaca cagtaaagaa aagtactgag cagacaatta
    34321 atgaaatgaa ggaacaaatc gtacatggac aagagacagg gaagctgtat ttgattattg
    34381 gttggatttc tatggtagta tcgttagcat ttattcctgt tgtatttggt gctgttgctg
    34441 ttattatggg ctacttatat cgagaaaaag atgagaaaat gggtacgatt ttgatgatag
    34501 caggggtagc tggagcaatc tttggtgtac ttttagggat gtcaatgtac tgataagagg
    34561 gataagagca aattattttg gagataggag ataaaaagtt gaataaagta aatatgaaca
    34621 agaagtttgt cattataggt attgttgtct tggctattat aggtattatt ttttcaaaaa
    34681 tgacaggtac atctggatta gagaagtcat caaaaccatt ggtaacacag attttacaag
    34741 aacagtacaa cttaaatcga tcatgtgatg atgtgacgat tactcatgaa aatggagata
    34801 acacataccg ggctaaagct atattagaca atggaagtgc tatcaacata aatattgagt
    34861 actatccaaa gaaagaccgt atatatgtgg agataccata tgctgaggtg ttaatgttaa
    34921 attgatttaa atgtattatg cataccgtcc aaacaggaac tcccaaaggt gtaaattcac
    34981 tagggagttt ttatagtttc ctataaatta aatactaata caggtgacaa aaaaatatta
    35041 caatgattgt
//
LOCUS       DACDTO010000019        35014 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDTO010000019 DACDTO010000000
VERSION     DACDTO010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.019"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(255..1229)
                     /locus_tag="IWK70_001000"
     CDS             complement(255..1229)
                     /locus_tag="IWK70_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9613566.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(1210..3093)
                     /locus_tag="IWK70_001001"
     CDS             complement(1210..3093)
                     /locus_tag="IWK70_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9613567.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(3111..5336)
                     /locus_tag="IWK70_001002"
     CDS             complement(3111..5336)
                     /locus_tag="IWK70_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9613568.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            5451..6485
                     /locus_tag="IWK70_001003"
     CDS             5451..6485
                     /locus_tag="IWK70_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9613569.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(6552..7565)
                     /locus_tag="IWK70_001004"
     CDS             complement(6552..7565)
                     /locus_tag="IWK70_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9613570.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(7643..7831)
                     /locus_tag="IWK70_001005"
     CDS             complement(7643..7831)
                     /locus_tag="IWK70_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9613571.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(8271..8399)
                     /locus_tag="IWK70_001006"
     CDS             complement(8271..8399)
                     /locus_tag="IWK70_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9613572.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(8402..9775)
                     /gene="rlmD"
                     /locus_tag="IWK70_001007"
     CDS             complement(8402..9775)
                     /gene="rlmD"
                     /locus_tag="IWK70_001007"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9613573.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(9886..10941)
                     /locus_tag="IWK70_001008"
     CDS             complement(9886..10941)
                     /locus_tag="IWK70_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9613574.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(10951..12381)
                     /gene="gatB"
                     /locus_tag="IWK70_001009"
     CDS             complement(10951..12381)
                     /gene="gatB"
                     /locus_tag="IWK70_001009"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9613575.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(12378..13850)
                     /gene="gatA"
                     /locus_tag="IWK70_001010"
     CDS             complement(12378..13850)
                     /gene="gatA"
                     /locus_tag="IWK70_001010"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9613576.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(13850..14155)
                     /gene="gatC"
                     /locus_tag="IWK70_001011"
     CDS             complement(13850..14155)
                     /gene="gatC"
                     /locus_tag="IWK70_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9613577.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(14261..16297)
                     /gene="ligA"
                     /locus_tag="IWK70_001012"
     CDS             complement(14261..16297)
                     /gene="ligA"
                     /locus_tag="IWK70_001012"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9613578.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(16314..18548)
                     /gene="pcrA"
                     /locus_tag="IWK70_001013"
     CDS             complement(16314..18548)
                     /gene="pcrA"
                     /locus_tag="IWK70_001013"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9613579.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            18703..20262
                     /locus_tag="IWK70_001014"
     CDS             18703..20262
                     /locus_tag="IWK70_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9613580.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(20373..22304)
                     /locus_tag="IWK70_001015"
     CDS             complement(20373..22304)
                     /locus_tag="IWK70_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9613581.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(22322..23236)
                     /gene="pfkB"
                     /locus_tag="IWK70_001016"
     CDS             complement(22322..23236)
                     /gene="pfkB"
                     /locus_tag="IWK70_001016"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9613582.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(23233..23985)
                     /locus_tag="IWK70_001017"
     CDS             complement(23233..23985)
                     /locus_tag="IWK70_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9613583.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(24144..24353)
                     /locus_tag="IWK70_001018"
     CDS             complement(24144..24353)
                     /locus_tag="IWK70_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613584.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(24364..25692)
                     /locus_tag="IWK70_001019"
     CDS             complement(24364..25692)
                     /locus_tag="IWK70_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613585.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(26057..26644)
                     /locus_tag="IWK70_001020"
     CDS             complement(26057..26644)
                     /locus_tag="IWK70_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9613586.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(26727..27977)
                     /gene="clpX"
                     /locus_tag="IWK70_001021"
     CDS             complement(26727..27977)
                     /gene="clpX"
                     /locus_tag="IWK70_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9613587.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(28224..29513)
                     /locus_tag="IWK70_001022"
     CDS             complement(28224..29513)
                     /locus_tag="IWK70_001022"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9613588.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(29667..30599)
                     /locus_tag="IWK70_001023"
     CDS             complement(29667..30599)
                     /locus_tag="IWK70_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613589.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(30756..31415)
                     /locus_tag="IWK70_001024"
     CDS             complement(30756..31415)
                     /locus_tag="IWK70_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9613590.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(31418..32443)
                     /gene="sppA"
                     /locus_tag="IWK70_001025"
     CDS             complement(31418..32443)
                     /gene="sppA"
                     /locus_tag="IWK70_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9613591.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(32456..32746)
                     /locus_tag="IWK70_001026"
     CDS             complement(32456..32746)
                     /locus_tag="IWK70_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613592.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            33155..34960
                     /gene="glmS"
                     /locus_tag="IWK70_001027"
     CDS             33155..34960
                     /gene="glmS"
                     /locus_tag="IWK70_001027"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9613593.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
BASE COUNT         9584 a         7464 c         6281 g        11685 t
ORIGIN      
        1 aatcgctcca aactgatgta agaattgtac ctagtataga cataaaaaaa tcgactgtca
       61 atcattttta tgaaactttt tttaattttc tgatagttaa ctttgttttt cagaaaaata
      121 acaattgatt ttattgaaga aaatagtcat gaacaaaagt atctgagaac ttttttactc
      181 tcttgttcat gacatataga ataaagcagc tttgtcttgt aaactccgtt tttcaaataa
      241 taatctaaag attgctactg aataataaag ccgatttcat ggtcctcttc gctgctgatt
      301 cggtaaggtg actcaacatc cgctccccag ctattgatac ccccgactcc tcttactttt
      361 gcaaaaatcg taagaaccgt tcgtctttct ggcggtagtt cttctatatg tgttgcctgt
      421 tccagttctt ctgctttgaa tggtaaagcg gaaaaagcaa atggcgtatc ctgctgttca
      481 aatcgcaacg aaaaatcttc ttgatcttta tccgtattat ccaacgtttg atttcttgtt
      541 agttctaccc atttcgtttc catatgcatg ccgcattctt ggggaacaag gtatggggaa
      601 acggggagtc ctgccacttt ataaatacct tcgacaccac cagccatccg atccggatat
      661 gtttcccctg ataggccttc atactcaaaa aattttgctt tcgttggcat aatgaacctt
      721 aaacccaatg cgggtaattc cggtaatcct ttttgtccat aataatgcat attgacaagc
      781 attttaccct ctccagcgat ttggtaggaa attgtcactt gggtttttgg tactgtgttg
      841 atttccagtg taaaaatgat ttcaaccgtt tcagcgtatt cattacttga atattgatta
      901 tttactggag caatcggcaa atcgatcttt ttattgtcca ccagtacttc tatttctttg
      961 acttttggat acaatccggc tcctaaccac ataccggatt taaatccaaa tccgctacct
     1021 ctgtcattat cggtcgttgc tcgccaaaaa actggaagtg tttcccggta tagccattct
     1081 tttccgtttt tattgagcga ttccagtccg cctcttgtat aagaaaaaat ataatgcgta
     1141 ttccccgatt tgacacctaa tgtacagtcg ccgaagacga ttgcaacttt ttccgtatcc
     1201 atatgacttt tatttactgt atttttcata ataatatctt acctcctgca tggcgggttt
     1261 ttcttgtcca tctgcaaaaa cgatcccatt ccctgagaat tcatagtcac ttggtttatc
     1321 gtcaaagtca ccgccatagc gcatcacttc tctgcctgtg attggatcag tcacttggat
     1381 cgcctgatct ttaaaatccc atatatagcc tccttggtac atttcataac gatcaagcaa
     1441 cgtcatgtaa gaatccattc cacctaacga attccccatg tcatgcatat attcacataa
     1501 gatgaacggt ttttccgctt tattttcaag gtagtcaatg atctcttctg gtggcgcata
     1561 catgcagctt tttacatctg aaatcgaatc ttcgaatgct ggatttggaa aattcccttc
     1621 ataatggaca agtcgatcgg ggtctacttt tttgaagtaa tcgttcattt gtcgaatgtt
     1681 ttcttccgca taagattcat ttccaagaga ccaaaaaaga atacttggat ggttttttaa
     1741 tgtttcgaaa ttcgtttttg ctcgatcaag aacagccgcc tgccactgag gtactgcacc
     1801 tggtacgttg taagaaggct caaatgcgcc cattttttgc catgagccat gggattcgag
     1861 gttcgtttcc gccatcacat ggattcctgc ttggtcgcat aaataatacc aagtgatctg
     1921 gttggggtaa tgagatgtcc gtacgccgtt gatctttgca ttttttatga tagccaaatc
     1981 tttttccata tcttctatag tgatggcgcg tccttttttc ggatgccact catgtcgatt
     2041 taccccgttg ataatcatcc gttttccatt gaagtagatg attttatctt tgatttcgat
     2101 tttacgaaaa ccgaaggaat agtcaatata ctccgttact cttttttctt cgtctatcgt
     2161 ctccaattca agcttatata aattgggctg aaaatgcccc cacagtgcca cattttcaaa
     2221 tccatcgatg cgaatcgtca tttgctcttt taacggatat tgctgttgat agatttcctt
     2281 cccatcagga tctagtatcg ctacattcag ctgactgttt ttggctactt gctcaaaaga
     2341 caaatctact tcaagtttgc cattttgaca tttgtcatct aagcgaggtt tgatccaaag
     2401 atcttctaaa tggttttgtc ctcgagcaac taagaagaca tctctgaaga tcccaaaaaa
     2461 tcggaaaaag tcctgatctt ccaagaatgc agctgtactt cttttgtaaa cctccacagc
     2521 tagaatattt tcttcttcca aataaggtgt taaatcaaat tcagacggag tgaacgaatc
     2581 ttcggcatac ccgataaaat gaccattcaa ccaaataaac attgcttgtt ctacaccagc
     2641 aaaatgaatc gagatttgtt ttccctttag accttcttct aatcgaaaag tcgtcaaata
     2701 cgaaccaacc ggattgtctt tagcagctga aaagatccct tccgcctctt tctcagggct
     2761 tgtcgtgaaa gggggacggc gaaatacatg tccctcccac ggatacatcg tattgatgta
     2821 atggatcttg tcataatttg ctaattcaat atgactagga acagtgattt catcaaaaga
     2881 acaggcatca aaatcttttt tataaaatgt ttttggtcgt accgaaggat ttggcgaata
     2941 acaaaaacgc catgatccat ttaaagactg tttaagggag gatgtggttt ctacttcttc
     3001 ccttgttgca taacaaacat gatcgctatg tgcatttgat tggttcactc taaaaacttg
     3061 tggatcatct aaccatttta tatttgcttt catacttttt tccttctctc tcataacttt
     3121 ttgaaatgaa gcagttctgt ttggaaatca ccttgaaaat cggggaagta aagtccaact
     3181 gtcatcaatt cggcccccga aaatatccgc ttcgaggtct gttcctgata aagtgcctca
     3241 tcatctaacc ccatcagata tacttcatgg aacgctggtt gagctgaagc tagtatcctc
     3301 cctaaaaaca caatagcttc atcttgttta ggagacacaa acatccatgc ggcaatattt
     3361 tcttcaaaag gactcgccaa acgataataa tggccatact ggatcaccgg acgaatcgtt
     3421 tgataggata taatttggtc ttttacttgc tttttttctt caggagacag ctttgtcaaa
     3481 tccagttcat aaccaaaaac agcgctcata gctgtttctc ctcttgtttt caacgaagta
     3541 gatctgtcgg tctgatgatt tggtacagct gatacatgag ctgtgatgga cgaaatcgga
     3601 taagcaaggc ttgttccata ttggattttc atacgctcca ctgcatccgt gttatcactt
     3661 gtccatgact gcggcatata ataaataaac ccggcatcaa accgtccacc tccaccagaa
     3721 cagccttccc ataagatttc cggataatca gttgtcaaat gttcaagtag ttcataaagc
     3781 ccgagcatat aacgatgcgc tacttctcct tgtctctgtg ggtcaagggt aatcgaatag
     3841 acatctgaaa gcgaacggtt catatcccat ttgatgtagt caagaggaat cgtatctaaa
     3901 atctttctca tttgtttctc aatcgtctgc cgaacatcta accttgtaaa atccagtaca
     3961 tgttgtgatc tggaagcaga aggcattcta cctggctcct gcataagaaa atcaggatgt
     4021 gttcgataca gttcagaatc aaccgagatc atttcaggtt caaaccataa accaaatttc
     4081 aatcctttgc tatgagcata atctgctatt tcacgtaatc catttgtcaa cttgccttca
     4141 tactcgaacc agtctcctaa tgaagaattg tccgcatctc tttttccaaa ccagccatca
     4201 tctaatacga acatttcgat gcctagctcc tttgcttcat caatgatcgc cttgattttt
     4261 tcactattga agtcaaagta agtggcttcc cagttattga ccagaatcgg ccgttcagca
     4321 aattgatgac gacctcttgc gattctttca cgtagcaact catggtggat ctgactcatt
     4381 ttgttcaatc cctgacttga gtaagaaagg atcatttcag gtgtttgaaa agactggcct
     4441 gcattcaatt cccacgaaaa acgatggctg ttgatcccac cgacgactct caattgattg
     4501 atctgatctt tttcaagttc gaaagaatgg ttgccggaat aaacaaattg aagtccgatc
     4561 gcttctccag tattttctgt cgtatgatga tgaactaaag caataaagtt attcatatgg
     4621 tgactgctcg ttcctcgacg gctttcaaat acttttgttc cagataaaac agattgccgt
     4681 gaaatctgtc tttcattgac atgtgctcct ggcaaagcaa tgacttcatc aaatcgccta
     4741 gtatgggcaa agtctagctg gaaactagcc gctttttcta gtttgattgt ttctccggtt
     4801 tcatttctta tctttacaga tcgtgtgatc actgctctat cagtaaatat ggtatagaac
     4861 aaatcgaaat aaatcgctgt ttctctatcc ttaagtgaaa tcaccaacgt ttgtgcctct
     4921 gacttgtcca tcacataagt ttgaggcaag ccttcgatct ctggcttccc atctgtaata
     4981 taatggctgt catatctcag atccagtaag tctgatccat tcttgcgctg aatgatcgat
     5041 gcgggcgttc gaaagtccat gctgccatga gaagagtatt cttgcggtag tgtatctttc
     5101 gacagagacc ggtcaggatt taagggaaca tttcctgaaa atccacgatc tcttcgggga
     5161 taatttttat tgtctttgta ctgcttgact ggtttgccaa aataaatatg cgccaataca
     5221 ttgctttctt ctatctttat cagataagaa agaaatgtat ttcgtaaatg gaagactcgg
     5281 ctcttttcat caaataagat gaaattttta tctgtttttc tatgcgtaat atccatcatt
     5341 tcactcccaa caaaacggaa accgtttact tttttttata attcctgtat actgtttact
     5401 aaacaatttt gtcaataaaa attttttatt tttttgagga gtgtgtaaaa atggttggaa
     5461 ttagagatgt cgcacgaaag gcaaatgtct ctcctgctac agtttcccgt gtgttgaata
     5521 acgatcagac gatccatgtg gctcagacta ctcggcaacg gatagtagag gcagctgctg
     5581 cgttggatta tgacatcagt actcgtaaat atatcaaaaa gagaatgcct tcgattggtg
     5641 tgatctctac gatcggcaaa gcgagcgaag aacaggatat ctattacaaa catttgcgag
     5701 tagggatgga agaagaagct cgacgtctgc atttagggat gaatcgaatc tataatctat
     5761 ctgataatcc taaacaatgg gaagatctag atcagttagg tgcggtgatc gttgttggga
     5821 ctgttacgaa agaatcgatt accgccttgt taaaacaaaa catgcaaata gtcgtcgtgg
     5881 ataaccctga catccagcaa gaggtcgata tggtgtatgc agatcttgaa cggatgacta
     5941 aaaccgtact ggaattgttc ttagaaaatg gtcataaaag aatagcctat atcgggggat
     6001 ataaagtcga tatggatgaa ttcggtggga agcagatttc tataaatgaa aagaggatgc
     6061 gtgcctatca gcattttatg atcgatcatc acttagaaaa atatatgtct tatcatctag
     6121 gtggctggac agaaaaacaa ggtgaggtac ttgcgactaa tctattagaa aatgaatccc
     6181 ctcttcctac tgccattctt gtcgggagcg atccaatggc aattggtgtc tatcgggcat
     6241 tgaaacaaaa aaatatccaa attggagaag agattgttgt agcaagcttt gataatatcg
     6301 agacaatcgc aaacctctcc cctggcttga ccagtgttca aatcaatgcg aaatcaattg
     6361 ggcaagcagc tgttcgaatg gcattggagc gaattgacgg tatacgggac gaaccaatca
     6421 ttttgactta tcctgcacga ttgattgtta gggaaagttt tatcccaaga acaaaaaggt
     6481 attgaaagag aggttgtgaa aagccgcttc gtttcttcat actcaaaacg aaacggcttt
     6541 ttcacaacct cttacttata cttattcaca aaatccaatg tctgctggta acaattttgc
     6601 gattctttta gcgtgtaatc aaattggtat tcatgggtaa tctctttttt tgtatctgta
     6661 taaaacaagg attgcacggg tacctttaat tctgtcaacc gattttctaa tgcttgacct
     6721 tgctcctgga atgagtatgc atttccgtca gttatatacg ttggcggaaa ttccttagaa
     6781 acatggtcag ccacactagc ttctttcaat tgctcactcg ttttccaatc ttttgttcca
     6841 atcaacgacc acgcaacggt tttgacgaaa aacttcataa atcgatcatc cgaatgctgc
     6901 tgtgctgtta gcttcaaatc aacaggtccg caataagaaa tggttccttt cagactatct
     6961 tttggaagta gctgtttgat tttcatttgt ttagcatacg catcattcgt ttgtatagct
     7021 gcatattgca atgcgatttg tgaacctgca ctatctccac caaaaaataa ttgttccatg
     7081 tctagcaatt tgtcgtcttt ttcttcagct aataaatctt tgatcagttc attcacttga
     7141 agaacttgat tagggtattc tgaatcaggc gctaattcat aattcatcgc aaccactgcg
     7201 atgttggcgt ctgaaactaa ttttgtcgca aattctttga cacccgattt atctccgccg
     7261 acgaatcctc caccatgtac ccaaaataga acaggaacag gccccttgct gttttttgga
     7321 tagtagatgt cataagtatt ctctttatac ttggactcat acgtcaaatc tgtttttttc
     7381 gccacgtttt tttctgcttg atcgaacgtt tttttatcag tgatttgaac agtatttgaa
     7441 aacatatggt tgatcaagaa tgctccaggt ctgggggaaa gctggaaggc aagaaagatt
     7501 gcaaacgcga tcccaagaat gattccgaag aaccatccga taaatttaag aattttcttt
     7561 ttcataaggc tccctcagta gttagtatta agcattctga ttataccata gtgttatttc
     7621 ctatcaagct ctcctagatg atttaatgat tttccccaaa cttcttctgc aatgcttttg
     7681 acaaaagaaa ttttttccat tgttcttcaa acaaaacatt ttcttcttct gttgaagcaa
     7741 atccacattg aggacttaag cacaaacgat ccaaaggcag atatcgtgtc gcttcgtaga
     7801 tccggtcaat cacttgctgg tgctcttcca tttcgccatt tttcgttaca gtcaaaatct
     7861 aagacaacta atttatcttc agagactttc tctaaaagaa caaaactccc agcacgttca
     7921 ctatcgtatt ctaaataata ggcacggaca ttttcttgag taaaataatg ggtctgctac
     7981 gtcgtcatcc cccctgatgc cgccaaaatc gaataataat ttccgcgtca aacatgtata
     8041 tttagtgttg aatcatttga gaatatgaaa ataaaaatct ttttgatcaa tcagttaatc
     8101 aaattgaaat aacgaaaaat ctgagacaga atgacgttca ttctgaccca gatttgattt
     8161 tgcacaactc aacagtcctt attggcaaat cgccaaaata atgtagacta actaaaaaga
     8221 ctaagaaaca ataacaaaag ttaacacttc ttagtctttc tttagcacgt ctatttatta
     8281 aagattttag ggccttttct catgttttta cgatcattct taggtgtttc catttgtttg
     8341 gatcggcctc caccttgttt ctttttgatc aattctttca ttttttcttt ttggtccatt
     8401 tttattctcc tttaacgaat gaagcgacta attcgatatg atgggtttgc gggaaaagat
     8461 ctacaggctg aactttcttc acggcataac ctgcttctgc aaataactta gcatctcttg
     8521 caaaagttgc cggattacat gagatataga cgattcgttc tggtgacatt tcgattgctg
     8581 attcaataaa atgatgatct aatccttttc gtggagggtc aactacgatc acagatggtt
     8641 tgatcccttt ttcttgccat tttttcataa ctttttctgc ttttccgact tcataatgaa
     8701 cattttcgat tttattattt aaagcattga actgtgcatc ttcaattgct tctggaataa
     8761 cttccatccc atacacctta tccactctgt ctgctaaaga caagccaatc gtacctattc
     8821 ctgaataagc gtcgatcaca atgtcttttt tatctaactc ggcaaactcg atggctgttt
     8881 ggtacagata ctctgcttgt tgagtattta cttgataaaa tgactttgct gagattcgat
     8941 aggtttttcc taatagctga tcttcgatat agtctctgcc atataaaact ttttctttgt
     9001 ctcctaaaat cacatttgtt ttttcttcat tgatattttg gatgaccgaa accacttctg
     9061 gcaattcttc atgaatagct tcagcaattt ccttggattt aaagaatttt tctttacgag
     9121 taactaaaac gaccatcatt tcatgggaat aatgaccacg acggataatg atgtgacgaa
     9181 gctgtccttc atttttttct tcattatatc ctcgtacttg gaaccgttgc aagatatctc
     9241 tgactacttt gattgcttga tcgatgacag gatcttggat ataaaaatct tcgataggga
     9301 ctagatcatg gctattcttt ttgtaaaaac cggtggctaa ctgtccatcg atacggcgaa
     9361 cagggatctg tgctttatta cggtagccga atggttctgc cattccgacc gttggaagta
     9421 caggaacttc tggcatctta gcaatcttcg ccattacatg ctctacttgt ttttgtttga
     9481 agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc gatacctatg cgaagtaatg
     9541 cattgttttc tacaggctga cgatcaggtg attgtgtaat aaatgtttcg acttttccat
     9601 atccaaactt attgcctgct tttactacta gtatttctac ttcttctcca gggagtgcat
     9661 tttcgataaa taatggataa ccatcgattt ttgctacccc cattccttca taactcaagt
     9721 cgacgatttc gactgtatat cgttcgtttt tctttactat ttgctgttct gtcatatttt
     9781 gctcctttaa gcctattttt caatcctact gaactctact ataaatcagc taattacaca
     9841 actaaaaccg gcactgacct tactacgttc agcaccggct tttgattaat tcttttgatt
     9901 aggtgtttcg tctgctattt ttccgtcacc gtcgatatct tcatccgtta aacgttcgac
     9961 ttcttttacg aattctttgc tgacaacctc taattcttct tcatctgtac ctgtgattgc
    10021 ttcatcagga atctgatcgg tattggcaaa gaactcaatg tgttgatgaa gattttggaa
    10081 atgcatcggt gcttccccgc cgtattcacc atctaagttg atcattactg gtcgattgat
    10141 atcatcttct gtttcaacga acaaactgct tgttttggta tagattattc ttggatcatc
    10201 gacatgtcga ccgccattca gcatgagtgc tgcaatatgc aagatctcaa agatatttgc
    10261 tgtcttcaca atgattaaag aaaacttccc atcatccaat ttggcatctg gcgcgatctg
    10321 ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac atcgaagcat ttccgatata
    10381 ttccccttca tcatacttca agcgcatttt gattggcttt actcgaggga gcatctctgc
    10441 tcctttcgca agataagcaa gataaccaaa aatactcttt agttctgatg gaacttcata
    10501 agtcaattct gtcaaatagc cacctgcagc gatattgatg aagtaactgt tatcagaaag
    10561 gccgatgtcc atttttacgg tttggtgttt ttttatcact tctgctgctg ctttaatatt
    10621 atctctagga atttttaatg ctcgagcata gtcgttcgtt gtaccagctg ggatgatggc
    10681 cattttagga cggtgttcaa gcggagcgat tccattaacc acttcgttga ttgtgccatc
    10741 gccaccagct gccactacta gatcaaaacc tagttctgcg actctacatg cttcattctt
    10801 ggctgaatct ggttctggtg tagttgcata ggcgctggct tcaaatcctg cttcttctaa
    10861 tgcctgtagg atatccgcca aatttttttt caataattct tttcccgagg tggggttata
    10921 aattacgcgt gcttttttca ttttcagcct ctatcgtttc gctaattcat ctttaagtag
    10981 cttattcacg acaccaggat ttgcttgtcc tttagttgct ttcatgattt gaccgactag
    11041 gaagccaacc gcacgatctt ttccattttt gaaatcatcg acagattgtt ggttgttatc
    11101 caaaatttca ttaatgattg gcagtagttt gttcggatca gataattgca cccaaccatt
    11161 tttttcaact acttcttggg cttctccgcc atttgtgatc aattcacgga agactttttt
    11221 ggcgattttt gagctgatcg ttccatcggc gatcaaacga atcattcctg ctaagttctc
    11281 aggtgtcaat tttgtctcag ctaattccag tttctcactg ttcaagtaag cagaaacttc
    11341 acccatcaac cagttagatg cttgcttggc atctgcacca ttttccaaaa cagcttcaaa
    11401 aaaatcagac atttcttttg taagtgtcaa taccattgcg tcatactctg gaagtccaaa
    11461 ctcacggata tagcgttcgc gacgagaagc tggcatctca ggcaaactag cttttacttt
    11521 ttgaatccat tcgtcatcga tcacgaattt cgggatatca ggctctggga agtaacgata
    11581 atcactagac ccttctttga cacgcatcaa aactgtttta ttcgttgttt cgtcaaaacg
    11641 gcgtgtttct tgttggattt ctcctcctga aagaagaact tttgcttgac gtttttcttc
    11701 aaaagctaaa ccttttttca cgaaagataa agagttcaag tttttcaatt ctgctttcgt
    11761 accgaactct tcttgtccat aagggcgaag agaaatattg gcatcacagc gcatagagcc
    11821 ttcttccatt ttcacatcgc tgacttctgt aaattggata attgatcgca atgcttctag
    11881 ataagcgtat gcttcttctg gtgaacgcat atcagcttca gaaacgattt cgatcaaagg
    11941 cgtgccttgt cggttcaaat caacgtaaga ataaccgcca ataccatgca tgttttttcc
    12001 agcatcttct tctaaatgga cacgttcgat ccggattttt ttctttttac cttctacttc
    12061 gatttcgatc catccgtcat gaccgattgg ttgatcaaat tgggaaattt gatatgcttt
    12121 tggattatct gggtaaaagt aatttttccg gtcaaaatga gtatctttag agatttcaca
    12181 gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca gctttattca ttactggcag
    12241 cacgcctgga taaccccagt cgatcacgtt tgtattgctg tttggttctg caccaaaatg
    12301 agctggcgct ggagagaaaa tttttgagtt ggtttttaac tcgacgtgga cttctagtcc
    12361 aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa tagctggttg ttgtttatgg
    12421 aattctgttg cttgttcaaa cgcatatgcc gcttgataca tcgttttttc atcaaaataa
    12481 ttaccgatga tctgcaatcc aactggtaat ccttctgata aacctgccgg tacagacata
    12541 cctggaagtc cagccaagtt gacagggatc gttaagatgt cactcatgta cattgtgatt
    12601 ggatcgttaa tgttctcacc gattccgaat gcaactgttg gtgaagatgg cccgatgatc
    12661 aagtcataat ctgcaaagac tttttcaaaa tctcgtttga tcaatgtacg gacttgtcct
    12721 gcttttttga agtaagcgtc ataataaccc gcgcttaagg agaatgtacc taacatgata
    12781 cggcgtttta cttcttcacc gaatccttct gaacgtgagt tgacatatac atcatctaat
    12841 gatttcacat tttcagagcg ataaccgtag cggataccat cgaatcgttg caaattggaa
    12901 cttgcttctg atgaggcaat gatataataa acagccacac cgtattttga atgaggcaag
    12961 ctgacttctt ccacaacagc acctaatgaa cggaatgttt ctgctgcttt caatacagct
    13021 tcttttacgc caggggccac accttcgcct aagtattctt taggtaaagc aatcttcatt
    13081 cctttgatgt cgcctgtcag tccctctgca aaatcaggaa ctgaaacgcc tgaagaagta
    13141 ccgtcttttt cgtcataacc gctgatagct gtcaatgcta atgcattatc tttcaccgta
    13201 cgggtcattg gtccgatttg gtcaagagat gaagcaaagg caatcaaacc aaaacgtgag
    13261 acacggccat aagtaggttt catccctacg atgccgttaa aagctgctgg ttggcggata
    13321 cttcccccag tatcactacc taaagaaact ggaatttgtc cagctgcaac tgctgctgca
    13381 gaaccaccag aagagccacc aggtactttt gttgggtccc aagcattttt tgttttcttg
    13441 aaataagaag tttctgtact tccacccata gcaaattcat ccatgtttag cttaccaaca
    13501 gggatcatat ctgcttgata gattttatcc atgaccgttg catcataaat tggttcaaaa
    13561 ttgtaaagga tcttagatgc agcggttgtc aagatatctt ttgttacgat attatccttg
    13621 ataccaattg gaataccagc aaggacgttg ctttcatcaa tccctttttc atctaaagct
    13681 tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga atgcctcgac ctcaggctct
    13741 gtttctttga ttcgttcaaa tgttgttttt gtcaaatctt gtacagtgat ttctttcgat
    13801 acaagcatat gatgcagttc ttctagagaa cgattgtgta attctgtcat tatgcgccag
    13861 cctccccatt atcgatgatt gcgggtactt tgataaagcc gtcttctgtt tccggcacat
    13921 ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg atcttgtcgc atcacgttta
    13981 ctgtttcaac cacattcgac gtaaatggaa caccttccgt atctacttct tccagcattt
    14041 caaccatatc gatgatttta cctaattggt cggtaaaact tgcaagttct tcatctgcga
    14101 acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc ttcactaatc gccatactac
    14161 cccttctttc taaacgagtt aaaaactttg ctctctattt taacacaaga gtgccgtgtc
    14221 tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt tcattcttct actgagcgac
    14281 ttttagccaa tgcatctgcc atttgatctt ctgtccaaac ttcgattcct aattcttgtg
    14341 ctttggtcag cttgctgcca gcatcttctc ctgctaccac gatatccgtt tttttcgata
    14401 cgctaccagt cactttcccg ccaagatttt cgatcgtctc ttttgcttct tcacgagtaa
    14461 atcgtgttag tttaccagta aggactactg ttttttcttt aaaaggagac tcgacttctt
    14521 gcaattgggc atttcgaagc cctttgtatt cgaaattgac gcctgcttgt tttaattcat
    14581 tcatcaattc atggacttct tcgttactga aataagtgac tacactgtcg gcaatgatat
    14641 caccgatcgt atcaagcgca ataatcgact catagtcact tttactcaat gtttctagat
    14701 caccgaaatg ttctgctaaa atttttgctg ctttcgctcc gacatgtcgg attcctaacc
    14761 cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc gattgctgtc aaaatattat
    14821 ttgctgattt ttcttttatt ttgtccaacg tcaataactc ttcttctgtc aatttgtaaa
    14881 gatcagcaac atctgctact agttttttat catacatctg ttctagtacg cgcggcccca
    14941 acccatcgat attcatcgca tttcttgaaa caaagtggtt caaaccttct ttcatttgag
    15001 cagggcattt aggattgatg caccgtaagg ctacttcttc atccaaatgg acaagttcac
    15061 ttccacaaac tggacaatgt gtaggcaatt ggtattcttc gctgtcttta ggtcgtttat
    15121 ctaacacgac ttgcgaaact tctgggatga tgtctcccgc tttataaatc agaactgtat
    15181 ctttcaagcg gatatccttc aacttaatat aatcaccgtt atgcaggctg gcacggctta
    15241 ctgtcgttcc tgctactcgg actggtgtca tgatagctgt tggcgttact acccctgttc
    15301 gaccgactgt ccattcgatg ttttcgatta gcgtttcaac ttcttctggc ggaaacttgt
    15361 aagctgctgc ccatctcggt gctttgatag taaaaccaag ttgatcttgt agcgagaatt
    15421 cattcacttt gatgacgatt ccatcgattt catagggcaa atccacacgt ttgtcatgat
    15481 attcttcaat gtatgaccac acttcatcaa tcgaatgaca tagacgtttt tcccgattcg
    15541 tgtggaatcc aattttttct agctcttcta gcgcatcata ctgggttttt gcttgcatag
    15601 gaccgaaatc agctactgtg tataaaaaag tgtccagatt tcttttcgct gtgattttag
    15661 aatctaactg acgcaaagaa ccagctgcgg cgttccgagg attcgcaaag atatcttttc
    15721 cctctgcttc tctgtcttgg ttcaattgga caaatgaccg ttttggcatg aaacattcgc
    15781 ctcttacttc gatgttcatt ggttctttta atttgattgg gaccgaccgg acagttttta
    15841 ggttttcagt gatgttttct cctacagttc catccccgcg agtcgcacct ctcacgaaat
    15901 ttccatcttc ataacgcaaa gaaacggaaa gtccatctat ttttagttca cagcaatagt
    15961 caactacacg tcccactgct ttttgtacac gttgatcaaa agctatcagc tcttctttgc
    16021 tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt gactttttca aaaccttcta
    16081 atacttttcc gcctactcgt tgagtgggag aatcagacgt gatcaaatca ggatattgtt
    16141 cctcgattgc tactaattct gcgtattctt tatcataaac atagtcttcc actgttggtt
    16201 tatcttctac gtagtattcg cgagaccatt ggttcagtct tgtacgaagt tcttctgctc
    16261 gtttagcagc ttcgtcaatc gtcatttcat gttccatcat ttaccctcca ctactaagct
    16321 ttttcaattg gcgcaaaggc agccaaaaga cgttttactc cttgacttgg gaaagccaca
    16381 tctagttcta gatccttcga cgtcccgctt actcggacga ctgttcctag accccatttt
    16441 ttgtgtttga ctttatctcc aggtttccag ctgttatttt cccctccagt agcactttta
    16501 gagggaacaa ccgctgtctc tggctgttta tagctatatg ctgttttttt agcgaataca
    16561 ggcgtttttt taggcgtagg acgcatgcct tcgatttcca gtaactcctg atcgatttct
    16621 tctacaaaac gagaaggtcg gttatattgc gtccgtccat ataatgttct ggagaaagca
    16681 tttgttaaat acaacgcttc ttctgctcgc gtgatcccca catatgcgag acggcgttct
    16741 tcctccagtt cgctttcttc catcaatgct cgagataaag gaaaaacccc ctcttctaga
    16801 ccaatcagga atacaactgg aaattctaat cctttggctg catgcaaagt catcaatgtc
    16861 acttgggaag catcttcttc taaattgtca atgtcggaaa caagtgccag atcatttaag
    16921 aagaccgtta gtttttcttc tggtgcatca gcgtcttctt cattttgctg ttcgaattgt
    16981 ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt tttcgatgcg tgcttgtgct
    17041 tctaatgtat tttggatttt taagtcttct agatatccgc tacgatccaa aacctctttc
    17101 gtcaattctg tcactgtcaa gtatgggatc atctgagtga cttcttggat catctctcca
    17161 aatgcaccta attgctgtcc tgcttttcca ccgatatttg ccagatccac attttgcgct
    17221 gcttcaagca gtggccattc atgaagagaa gcaaaactgc gtagtttttc gatagatcct
    17281 ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa aactgattga atcttgagga
    17341 tttaccagca cattcaaata cgctaaaata tctttgattt ctttacgatc gtagaacttg
    17401 tgccctccaa ccattttata aggaatattt gctttcaaca gagtttcttc cattacacga
    17461 gattgtgcgt tcgtccggta caagatggca aagtcgccgt aattccgatg attgcttcga
    17521 atctcttctt gcatccggtc tacgataaaa cgggtttcgt ctcgttcatt atctgcacgg
    17581 tagtaagtga tcttgtttcc ttctttgttt tctgtccaca agttttttgg ttttcgattg
    17641 ctattgtttt caatgacttg gtttgctgcg ctaaggatgt ttttcgtgga gcgatagttt
    17701 tgttctagca aaattactgc agcatctgga taatcttttt caaaatccaa gatgttctgc
    17761 atatctgcac cgcgccagcc gtaaatactt tgatctgcat ctcccacgac gcacaggttc
    17821 ctaaatctac ctgccaataa attgactagc gtatactgtg cgtgatttgt atcttggtac
    17881 tcatctacat gaatataatg gaatttgttt tgataatacg tgagtgaatc aggatgttct
    17941 tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt ccatacattg attatattga
    18001 agctcttttt ggtaagcagc ataacatttt gccgcaatct cttcgtaata actgccttgc
    18061 atttttgcaa aatcctgagg ggtctgcagg ctgttttttg cttgagagat tgttcctaat
    18121 atacttcgag gatcatattt tttcggatca atgttcagtt cttttagtat ccgtttcatc
    18181 aaggtcagct gttcagaaga atcgatgatt gtaaagttcc ggttataccc gataaaatca
    18241 acatctcttc ttaaaatacg cacacacatc gagtgaaaag tcgataccca gacatcttcg
    18301 cctccagatg cgagaatcgc attgacacgt tctttcattt cttttgcagc cttattggta
    18361 aaagtaatcg ctaggatgtt ccaaggattg acttcttttt cctcaatcaa ataggcaatg
    18421 cggtgtgtca gcactctcgt ctttccactt cctgcacctg ccattagcag taatggtccg
    18481 tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc cgtttaataa ttcagctttg
    18541 gatgacatta acaacatcct ttcttttatc aatcttgtta attatagcat tttcttggct
    18601 ttcattaaac tagctagttc aaaatcattt gtttcttttt atggattttt ccttgtcaat
    18661 tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt gaatggaaaa aaaaggaaaa
    18721 atcaagaaat tagataagac tcagatatat tttattctaa aaggaatatt tgttggactg
    18781 ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg aaaagctaag cgatttagtt
    18841 cgaagtatct atgaaatgag tcgggaacag ccgatttact taattggcct agctatttgc
    18901 tgcatccttg cagcattttt cgtgggttat ctggtcaaaa atgaaccgga tatcaaggga
    18961 agcgggattc cacaagtaga gggacagctt cgcggggaat tatcaatgaa ctggttctct
    19021 gttttatgga aaaagttcat tggcggtgta ctttcagttg gtgcgggcct ttttctaggt
    19081 agagaaggac cttctataca gcttggcgca tccattggac aaggagctgg acaattgttt
    19141 cggagtccat cttcagaaga aaagatttta atttctagcg gtgcaagtag cggacttgca
    19201 gcagcattca atgctcctat cgcaggatta ctctttgttt tagaagaaat ccatcactcc
    19261 ttttctccac ttgtttggct gacatcattt gcttcagcaa tcactgctaa cttcgtctct
    19321 ctttactttt tcggcttgag acctgtttta tatctaggtg accttccttc tttgccgttg
    19381 aaatattatg gttccttagt gatcttagga gtacttctag gcatcttagg ttttatttat
    19441 cagaatgtct tgctggcttt gccaaaattt tacaaccgat tgcctttacc agcttatttt
    19501 cacgggttcg ttcctttctt gctgattttg ccaattggtt atttatggcc tagtactcta
    19561 ggcggaggca atcaaatcgt tttagctttt ggacatgctt ccttgccaat cattatgatt
    19621 atcggtttgt ttgttcttcg attcgttttt tccatgattt cttacggtgg gggtcttcca
    19681 ggggggattt tccttccgat attgacattg ggggctttaa tcggtagctt ttatggaaac
    19741 attatggttg atttcgggat ggatccaatc tacatcaagg attttgtcgt tattgccatg
    19801 gctggttatt tcacagcgat tggaaaagca ccgctgacag caattatttt agtgacagaa
    19861 atggttggct ctcttaatca tctgatgcct ttaggattgg ctgctcttgt tgcttatatc
    19921 gtcaatgacc ttctcggcgg taatccaatt tacgaatcat tattggaacg tctacttgga
    19981 aatactcaga ccgcctcact aaaaggtgca aggacaactt ttgattttcc agtaacggca
    20041 gagagttctt tagacggatt aatggtacga gacttcaatt ggcctaaaaa tatgctgcta
    20101 atctctatac gcagaggaaa tcaggaaatc ttgactcacg gtgatacgat catgaatgta
    20161 ggagatattt tggttatctt gactgatgaa gcaaacttgc caagaatcaa gcaggaaatc
    20221 gaggctaaat cttctatcac aaatttgaag caatcatttt aacgaacatg gaaaaactat
    20281 tttacaacaa ctattcatct acactacttg attctatatg attctataaa aaaagaagag
    20341 gctgggaaaa cccagcctct tcttttttat ttctatgctt ctgctactgc aggagctttt
    20401 ctcaataaac cataaatcac accagatacg actgctccga tagcaataaa caccaaatac
    20461 atgaccgggt gactcagtaa gaatacaacg aaaatcccgc cgtgaggagc cagtaatttg
    20521 attccgaaag ctccgactaa tccgccagtt aaagcagaac caataataaa acttggaatg
    20581 gcacgaatcg ggtcagctgc tgcaaatgga atcgatcctt ccgtgacaaa cgatagtccc
    20641 atcacgatgt tcgtcaaacc agcatcttga tctgttttgc tgaatttatt cttaaacaaa
    20701 cgtgtagcaa cgaaaattgc caatggaggt accattcctc cagccataac agaagccatc
    20761 acgacacttc cgccagttgc aacagtcgta gctaaagttg ctgtaccaaa cacataagcg
    20821 gctttgttga ttggaccgcc taaatctgcc gccatcattc cagctaataa tgcaccaagt
    20881 aaagcagcat tcgaaccact catacttgtc aagaaaccgt tcaatgcatc attgattgct
    20941 ttcataggga cattgaccag caacatcaag aaaccagtaa tcagtaagcc aaacactgga
    21001 taaaagagga tcgttttaat tccatccaat gattttggta agttcttgaa taatttacgt
    21061 aaaaatacga tgacataacc agctaagaat ccaccaatca gtgcacctaa gaatcctgca
    21121 ccgcctgtat tagctaatgc accagcagca aatccaacga tcaaaccagg gcggtcaccg
    21181 atacttgaag caataaatcc tgctaatacc ggcagcatga aaccaaatgc agcttgtccg
    21241 atttggttga accagctagc agcttgattg taattcccta gatttgataa ttgatcatgt
    21301 ggcacgccaa taaattggtc gatcatgaaa gaaagcgcaa tagcgattcc tccaccaata
    21361 acgaaaggta acatgtgtga tacgccgttc atcaaatctt tgtaaattct ttgtccgata
    21421 gtaccatccg catttccttc ttctgaactg ctttcttttc catcaccatg gaatactggt
    21481 gcagtaccac taagggctag attgatcaac tcttctggtt tacggatacc atcactgaca
    21541 ggacgattga tcagttcttt tccgtcaaag cgattcatct ctactttttt gtctgctgca
    21601 acgattacac cttctgcacg ggcgatgtct tcagcagtca atcggttttt gattccttct
    21661 gaaccgttcg tttcgacttt gatctccaca cccatttctt tcgctttttt cttcaaagca
    21721 tcttctgcca tgtaagtgtg agcgatccct gttggacaag ctgtaacggc aacaacgaat
    21781 tttctttctg gatgagcaga agcttctgca gcttgtgttt cttgttctgc tttttcttct
    21841 gcttcttttt gctcttcagc tgtttggaac aagtcttgga cttcttctgg tgtatttgct
    21901 tcttttaatt ttcctacgaa atctgcatca atcagcaatc gagatagtgc tgctaatgct
    21961 tgcaaatgtg tgtcgtttgc gccttctgga gctgcgatca tgaaaaacag ataagttggc
    22021 tgtccgtcta aggcttcata atctactcct ttttggcttt ttgcgaataa aactgttgct
    22081 tctttaactg cactgttttt cgcgtgaggc atggcgattc catcaccaag acctgtagaa
    22141 gtttgtgctt cgcgggctag aattccttct ttatatgttt cgatatcgga aatccggccg
    22201 gcatcaaaca ttttttggac catttcatcg attgcgcctt ttttatcagt cgcttgcaaa
    22261 tccatgatca tcgcttcttt gaccattaaa tccttgatat tcatgattat cctccttgaa
    22321 actaatcaat ttttgtgatt tcaacttctg gcagcaactc atcaatcaat tcgcgagctg
    22381 ccaagtcatc agaaaaagct gttgcgcttc cgcaagcaac tccccattta aaggcttcga
    22441 ttggatcttt cgtacggctg taagccccaa taaagccagc aatcattgaa tcccctgcac
    22501 ctactgaatt tttcaatggg cgaactaata cattcgtttt ataagttcct tgttccgtga
    22561 agagcaaagc accgtctcca gccattgaga tcagcacatt ctgtgcccct tcagctagta
    22621 attttttccc atacggaatg atttcttcta cagattggaa ggttgtgttg taaagctcag
    22681 ctaattcgtg attgtttggt ttgatcaaca acggtttctt aacaagtgca ttttttaggt
    22741 catcacctgt tgtatcaatc acgaaatctg cacctttttc tcgaatgata tcaatcagtt
    22801 cttgataaaa tccttttcgc aggctagctg gcgcgcttcc tgacaatacg acgatatctc
    22861 cttgtcctac tgttcctaat gcttgcttca actcatcgat ttcttcatct gaaatagatg
    22921 ggcctaagcc attgatctca gtttcagtgt ctgattttag tttgatattg attcttgtat
    22981 cttcttttac ggttgtgaac aaagactgga tttgttcttt ttcaagccac ttcgaaataa
    23041 attcgccagt gaaccctcct aaaaacccta gtgctgtaga ttgagaatcg atccgtttca
    23101 aaatacggga aacattgatc ccttttcctc caggtaactt gaagtcttcc ttcattcggt
    23161 tcaaatcgcc tagttttaac ccatctacgc gtacgatata atcaattgat ggattaagtg
    23221 ttactgtgta aatcattgta ttgcctcctt aattgttgtc ttttcacgga attcattaac
    23281 gaattctaat ggacaacgct cggttatgat cgtggctgct tccaaaggtg caacagaggt
    23341 gaaggacaac tggttgaatt tggtatgatc aatcaataca aatgtctctt cggaattttt
    23401 catggctaaa cgtttaacag ctgcctcttc tggatccgga gtcgtgaatc cttgttcaag
    23461 atgggcaccg ttcattccca taaatgcttt attaaaatga aaatttttta gttgttctat
    23521 cgcattagat cctaaaacag cattagttgc cagctttatc gttccaccta aaataatggt
    23581 agcaagctcc aaatcaacta aatttgccgc atgttgaacg gaatttgtca ctactgtgat
    23641 ctgttttcct tgcagaaaag gaatcatttc taacgtagta gagccagcgt ctaaataaat
    23701 gacatcttcg tcattaatgg tttcagctgc cgatttggcg atttgctgtt tttcgtgaac
    23761 gtttttgagt gatttttcac tcatattctg ctcaaaattc aataattgct ctttcttagc
    23821 tccgccatga actctttcta gaaacccact attctccagc tcttgtaaat cacgtcttat
    23881 cgttgattca gaagcattca aaaggtttac aagatcttgt gatttcacga ttttctgctg
    23941 ttcaagcaag tctaggattt tttgtctgcg ttcctctgta agcattttca cgatcacctc
    24001 aatacaaata atagcatatt ttttttctac tagcaatata aatcagtcaa aaacgttcat
    24061 aaaaattata aatcagtcaa aaaaaaataa aaaaccagtc agaaagactg gttttttatt
    24121 tttttgtcta tcatcaagcc gttttatcgt tttttatttt cgcttcttgt ttttttcata
    24181 taaacagaag caggttttct ttccttatga agaagataat aataaagtcc catcccgcca
    24241 aacatgacca aaaaaccgat gactgatcga ataggaaaat taggaacaaa aaagagacag
    24301 acggataata atgatgccgg aaccattatt cgataataga gttgtttatc cattttcttc
    24361 acctcatatt ttttgtaaag taatcaattg ttctaattct cggtaatcta aaaattgcac
    24421 acgcagatgc tgctcacctt tcttgacatc gaattggata cttcgccgct ttttctttat
    24481 tcgtttgaag gtaacatcag agagtctatg tttgatttcc tttaacggta attctgacag
    24541 ggattctgaa ggaacataga ttcttaagga taaaagatac tcttgatatt tttcatctgt
    24601 tacctctact tctagatatt ccctaatata ataacaataa gcttcgattc cattatccaa
    24661 agcaacagtc ggatcgataa ctttatagca catcatgcgt gatcgattct cgtcaaccca
    24721 aggaaaatac gcctgagcat ttagtgttcc gcgaacacgc ctttctttat aatctccttg
    24781 ttggatatca taactttttg ttacaaaaaa tgaagcattc tttttaggaa caggttgcat
    24841 ttgtgcattc tgatactgga cagattggat cgtatggtca gtgtaaatgt attcaacgaa
    24901 tggcggtttc aataaaccga caccattgtc ccaagcaaga tgatcaaacc caagctggtc
    24961 cctaaaatag gattgaaaca tttgattgtt tggcttttgc cagttaaagg ggtctcgata
    25021 aagctggata catatgactt cgttttctaa ttccttcact ttttctactt gttggatgtc
    25081 gagaaaaatc ggtttaggga tgatttgatg ataatagcga ctataataca tttcccaaca
    25141 cgaagtgaaa cataaacctt ggtaaaaaag atcgtatcct ggaaattggt tgcaatcgac
    25201 tactacttcc ccgttttgtt gtttcatttt aggtaaatgt tcgatttcat cctttttctc
    25261 aaacaatagc cgttgagaga tctgttttgt attgtgatag ataaactcgc tataagctcg
    25321 catataggcg aacaccccat gagcaatcat ttttttctga atgaagtctc gaatcaaaaa
    25381 ttgcttttct ttgaaaacag agatagacaa tacatttttt atcaataatt gattatcttc
    25441 caagcgaaaa ctacattcgt tctcgtcatt tcctacaact aaccaacttt cgtctcttac
    25501 cgatgctagc attatttttt cttgaagctt tcgaagatca tacggatccg cttgttgtgt
    25561 gaaagagcgg ataaatgttg gctggatcaa cagacgtgtc aaaatagccc aatcgcttat
    25621 ttcctgtttt ttttcttctt catttaagtc tgtgagctgg atagtcatca ataatttatc
    25681 ttcgccaatc attttctcac ctctttcaaa tctttttata atttaattat aaccaatgat
    25741 aaagaaaaac taaaacttta tgtcttagaa accaatttgg acgattacat gatagactaa
    25801 gtaaaaagaa acaaagaggt gtttacaatg gggttgaaat ttgaacgtac agataattta
    25861 gataaacttt tcgaatcatt tgctgtcgat ccggaaaaaa cagttgcacc aaaagaagag
    25921 atcgatcgat ttctaaaacc acagccacaa aatgaaaaga aaaaagacaa gaaacaaaag
    25981 taagttaagc tcaaaaaaag acttaccagc caaactttta tgaaagagtt tgactggtaa
    26041 gtctttttta ttctttttat gattcaattg cttgttcgat tgctgcccac gcttcttctt
    26101 ttccctgttt tgtaacggaa gaaaagagaa taaacgtgtc attgcgatca aaatccaacg
    26161 cttttttgat ttcgctctca tgcttgttcc atttaccacg agggatctta tctgccttag
    26221 tggcaacaac aattacaggg atatcataat acttcaaaaa ttgatacatc tggatatctt
    26281 cttgagaagg cttatgacgc aaatctacta aagaaacgac cgcacgtagc tggtctctag
    26341 tcgttaaata tgtctcgatc atctttcccc attttgctcg ctcagttttg gatacttttg
    26401 catacccata tcccggaaca tcgacaaaat gtaatgcgtt ctcgatcaaa taaaaattca
    26461 atgtctgggt ctttcctggc ttaccggaag tacgggctaa attctttcga tcaatcaatg
    26521 tgttgataaa agaagatttc cctacatttg atcgtcctgc cagagctatt tctggcaatt
    26581 gtgtatctgg atattgtttt ggtgccactg cacttatgat aatttcagct tgatggactt
    26641 tcatggtctc tctccttaac aaggtgatga ataaagcaag cggctgtcgc aaaagccaaa
    26701 atgactttta cggcagcctc ttttcatcaa cccgcttttt tctctgaatc tttatagatt
    26761 actgttggct caccagacaa ttcagctgct tcttttgtga taatcacttt ttcgatgttt
    26821 tcatttgaag ggatatcaaa catcacatcc atcatgattt cttcaatgat ggaacgaagt
    26881 cctcgagcac cggtattgcg ttcgattgct tttttcgcaa tggctctcaa cgcttcaggt
    26941 tcgaattcaa gttcggtatt atctagagaa agaagttttt gatattgttt gactagcgca
    27001 tttttcggct cagtcaagat ccgtaccaga tcatctgttg tcaatttctc taaagctgcc
    27061 ataacaggta atcgtccgat aaactcaggg atcaatccga attttagtaa atcttcagga
    27121 atgatatgct gcatgacact ttcgtcttca gaaagttttt ggttttgtgt accaaatcca
    27181 atcgtctttt cgcccatacg attcttcaca atcgtttcga tgccatcaaa tgcaccacct
    27241 acgatgaaca atatgttcgt cgtatcaatc tgaataaatt cttgatgtgg atgtttacgt
    27301 ccgccctgag gtggcacact tgcaacagtt ccttctagaa ttttcagcaa ggcttgttgg
    27361 acaccttcgc cggaaacgtc acgagtaata gatacatttt cgcttttacg cgcaatttta
    27421 tcgatctcat caatatagat gatccctttt tctgcaagtt ccacattgta atcagcagat
    27481 tgaagcaatt taagcaaaat gttttctaca tcttccccca cataaccagc ttctgtcaaa
    27541 cttgtcgcat ctgcgattgc aaaaggtaca ttcaatgttt ttgctaatgt ttgtgctaaa
    27601 aatgttttac cagaccctgt aggtccgatc aagcagatat tgcttttttg cagttcaaca
    27661 tcatctgtgt tttcttgtgc ttgctgattg acacgtttat agtgattgta tacggcaaca
    27721 gctaacgagc gttttgcgcg atcttgtcca atcacgtagt tattaaatac ttcaagaatt
    27781 tctagtggtt tagggacttc agtgaattcg cgtacagctt cttcataaaa ttcttcatca
    27841 atgatttctt tacataggtc gatacattca ttacagatgt agacccctgg accagctacg
    27901 atctttttca cttcttcttg ggttttcccg caaaaagaac agcgtacggt ctcattgcta
    27961 cttggattgt catacatggt cttcacccct taacttgcaa aaagtatgca agaaactatc
    28021 aaagacttta cagtctttta ctatcataac atagtttagt aaaaagaaaa agtgtttaac
    28081 aaaggatcaa ctgacctata cctttgcatt ttttactctt aacaaaaaat aaggaactgt
    28141 gacaaaatcg tgtcacagtc ccttatttcg aataaacaat tttctagaag tagcttcttc
    28201 agaaatgatt tattacttga tttctattct ttcgcttctg tcgctgtacc tgtgatcaat
    28261 tcgattgctt ttttcattgt cacatcatgt tccagcatat cttctgtcaa cacgcgtttg
    28321 atttgttcga ttggcatgtt gtatgtttct gataattctt ggatttctgc attgatttct
    28381 tcttctgttg cttgaagatt ttcagcagct gcaattgctt cgatcacaag atttgttctt
    28441 gtgcgcattt ctgcttcacc ttcgaattgt ttatgcaagt cttcttctgt agaaccagtt
    28501 aattgatagt acatttcagg agagatacct tgacgttgca tgttgttcaa gaattcgtcc
    28561 attgaacgat gtacttcatc atgaaccatc acgtgtggca attcaacgat ttctgcgttt
    28621 tcaacagcca tacggatcgc tgcttcatct tttgcatcgt ccgctgcttt ttcttttgtt
    28681 tctactaatt ctttgcgata tttttctttc aattcgtcta atgttgatac ttcgtcatca
    28741 acatcttttg cgaattcgtc atctaattca ggcacttctt ttgcttttac ttcatggatt
    28801 gtcactttga agactgcttc ttttccagca agatcttctg cttgataatc ttctgggaaa
    28861 gttacattta catctagact ttcacctgat ttatgaccaa ctaattgatc ttcaaaacct
    28921 ggaatgaatg agcctgaacc taattcaagt gagtaatttt cgccttttcc gccatcaaac
    28981 gcttcgccat ctacaaagcc ttcgaagtcg attactactg tatcgccatt ttcagctgca
    29041 gcatcttctt tgataactaa ttcagcttgt gcttcttgtt cacgtttgat acgtgcatcc
    29101 acgtcagcat ctgttacttc acggtcttgt ttttcaacaa ctagatcttt atattgtcct
    29161 aattttactt ctggtttcac tgttacttca gcagtgatta cccagtcttg acctttttcc
    29221 atgctttcaa catcgatttt tggttgagaa acaggatcga tgccagcttc tttgacagca
    29281 gcttcataag cttctggtaa tacagcattc aatgcatctt catataaagc ttcttcaccg
    29341 tacatacggt tgaatacttg acgagaaact ttccctttac ggaatccagg tacgttcaag
    29401 ctttttttca ctttattaaa cgcagttgtt aatccttttt ggatttcgtc ttgcgcaatc
    29461 gtaaatttta aaacaccatc attcgtgcct tttttttcaa atgttgcagt catttaattc
    29521 cctccgagtt tagaaattca ttttatacat caaaaacgtt tcatgcatac ctttaaatag
    29581 tacactaacc ctttcaaatt gtaaacatat tgaatggata gaatccttgt ttttcataac
    29641 atttttatct ttttcactga taaaatctaa tggaagaaga tcatttgctg caaaattttt
    29701 tctctttttt ttaaaaaatc ttgtttttca tcttctgtac cttcatacat cgtttttcct
    29761 gtacacgcct ctacagtcag ttgaaaccag cgcttcgggt cttcgatgta tgtatcatag
    29821 acaggataaa caattgcaga ttgcaacgtg aattcttctt ttaacacgga actaagcagc
    29881 gcatcttcat tcccgtaacg atctgaaaat acacataatt gctggtaact tgcctgctgc
    29941 tgcggctttg gcagatcttt tggtatgact ttgactaact tttcttcaat cgtcaaatag
    30001 aaaacaggat ggcattcgtt caattgccta agtgtctcca gtaattttgc ccttgctaaa
    30061 atatggacct ctggcgatac aataagttta ctcgctgttt ggataaactc atgataagga
    30121 agctgttcga tctcatcaat agacgcaagc tgacgtgcag gttccaacga aggaagttcc
    30181 tccgatttat tgtggagtgc cttgatcttc tgcctctcca tttgtccgga taattcttct
    30241 gcaaattgga ttttttcaat gacccgctgc tcgaaagctt cataaaaatc tgtacttgct
    30301 atcaattttc tagcatataa aaaatcttgt gtactgatta acaagtcaaa ataaaattca
    30361 gcaaactcct cattttgaag atactgtttt tcatgcaaga gtgcttgctt caatgcttct
    30421 tttttctcgt ccaactcgta aagacaaaaa acaagcaggc tattagcctc aaagctttct
    30481 ttcaactcgt aagctcgttg aaataattct tttgcctgct gatattgctg gtttgctagt
    30541 gcttgttgtg ccactcgcat taaattctca tatttttctg gaaaatcaat cggcttcacg
    30601 cagtgtcctc ctcttgctca tattcagctc atgttcaata gtagcaaaac aaaaaaagca
    30661 tgacaattaa aaataactga agcaccctct aaaagctaga ttttagatct gacttttaga
    30721 aggtgctttc aactgttact gctttttatt gctcatcagc tgttgacttc ctggttgaac
    30781 gctttgatca gattgatcgt aacaacactt gtattagtga aaaaatcgcc cagatgttgc
    30841 tttttctgat taaagactgc tggaagatac cctaacatca gcaaaggaaa ttttagaatg
    30901 aaccggcaag caccttctct aactaaaacg gttgaccagc taagttcttc ttcatcaaag
    30961 ctgatgactc gaataccaaa gatcattttc ccaatcgttt ggccatgatt gaatttcgtc
    31021 aataaaataa aatacgctag ataaataagt aaggctgtga atccgtagag actcagataa
    31081 gaatctgaaa aattaagcca acctagattt ttggcaatac caagtgttaa acgcgtaata
    31141 gctccgatac acaaaagatc gaccaagaag gcaaacattc ggataaaaaa acctgcatag
    31201 aaatacttag gaaaatcatt tgcttttggc ggttcatttc ttgtgtccgt atactttttc
    31261 catttacgtt gtttcttttt gatttcttcg ggcgtcaaag gctctctttc aaaagagctc
    31321 agtacctttt gggaaaagtt ggagtcaggt cctacagcag gctgctgttg cttatctttt
    31381 tcttgttcct tattttcatt tgaaagctct gacatgctta ctcacctccg taataataca
    31441 ttgcttttgg tgattccgct gtccctagat tctcaagaac ggataagatc tgactagtct
    31501 cagaaggctt caatccctgg aattctgcta gtttacttcc taaccatgta ctagcaaacc
    31561 cagatggcgt ggaagaatat tctactagtt ccgcatcttc aagctgttgt tcctttcgta
    31621 tagcttttaa agcatcttca ggaaatccca attcatcgac taaacctact tcttttgctt
    31681 ggacaccatc ataaatcctt ccatcagcaa tttttttgac ttcttcttca gatttattcc
    31741 gtccctcgct gacgattgct acaaaacgac tatatgcgct gtcgatatat gcttgaagca
    31801 ccgcttcttc ttcttttgtc tctggtcgcg ttcctgaacc catatctttc aacgctccgc
    31861 ttttaactgt tgtatcttcc acacccagtt tttctagaag tcctgaataa ttcaatcctg
    31921 acatgatcac accaattgag ccggtcactg tttcttctgt tgcaaagatt ttatctgctt
    31981 gagcagaaat atagtagcca ccgctagctg ccatgttttt catgctgaca tacattggga
    32041 tatcatgttc tttacgaatc gtatccaatt tcttagcgat ttccgcactt tcgtagacac
    32101 cgccaccagg agaatttact tctaaaaaga tacctttgac tgttggatct tcttcaatcg
    32161 ccttcagttg ttccataaaa tttttatgat tgtaaccttc agaagcaaat aaacctgaag
    32221 accctcccgc agcaatcgtt ccatctactg ataattttac gattttttgc gtactgtctc
    32281 cctcttccaa cacatttgga gctagttctt catccccata taaccaactg ttcaatccct
    32341 caatttgatc gttttcctga ggcttagtca aactagctga aactaacgaa accacaaata
    32401 atcctgcagc aatcagaact gctgcccaac gtcttttatt cattctttct cctttctaat
    32461 acaagttttt tatgacgatc acagtttctg actgctcaga agtgtcgaac cgctctgctt
    32521 tttcttcccg cgcatacccg gaaaacgact cttttgttaa ttcatcttct accgtaaact
    32581 cacgagcttg agcaacttcc aaggcatcag gtgcgatttc ttggatgcta tcccagccag
    32641 tatctaaaaa tattttcgta tcatctgata caccagctga tgcttcgaat ttggacaatt
    32701 gtttttttaa cattcccagt gtcatgatct caatttttga atccattttt actcctcctc
    32761 tatccgatta ctttctattg taccgaactc ctgaaaaaga tgatagataa aaacccatta
    32821 ttttttgaca taaaaaggac atttgacaaa gaatttgata tataccaatt tattttttat
    32881 tctttttttg ttgacaagaa cattcgcaaa aggttatgat tttaaatgtt tccagataaa
    32941 aaacaaaaag gaggatagtt atacctttta ggatagcgcc aggtctgtgt aaacagatga
    33001 cgaggagagg gtttatcgaa agattcggcg gatggcccta gggactgcac tctacagaaa
    33061 atccacaaaa aatgtctgca aagataaaac aaaaggattt tcctgcagct ggaagcattc
    33121 atacgaattc attatagaaa gtaggatgtt ttttatgtgc ggaattgttg gaatgattgg
    33181 attaaaaaat gtaacaccag gattgattga cggtctagaa aaattagaat accgtggtta
    33241 tgattcagct ggtatctttg tctcagatgg cacaactgat tatctggtca aagcacaagg
    33301 cagaattcaa aatttgaaaa acaaaataaa tattgatact acaggaaata taggtattgg
    33361 tcatacacga tgggcaactc acgggcaacc ttctgaagaa aacgcccatc cacatacttc
    33421 ccaaagtgga cgcttcgtgc tcgttcacaa cggcgtaatc gaaaactttg aagaacttaa
    33481 aattgcttat ttagcaaatg accactttat tggtgagacc gatactgaga ttatcgctca
    33541 tctaattgaa actttcgcga aggatactac aacgcaagag gcgtttttaa aagctttgcg
    33601 ggtcatcaaa ggttcttacg ctttcgcttt gattgatcgg acagcacctg atgttattta
    33661 cgtcgctaaa aataaaagtc cactattaat cggactcggc gatggattca atgtgattgc
    33721 cagcgatgca atggctatgc ttgctcatac gaaggaattc gttgagattg aagatgaaga
    33781 gatggttacc gtcacatcag aaaaagttat tattcaaaat tttgcaggtg acatcgtaga
    33841 acgttcttca ttcgaagcac aagtagatgc ttcagatatc gagaaaggta cctatccttt
    33901 ttatatgctg aaagaaatcg acgaacagcc tattatcatg cgtcgtatcg ctcaaaaata
    33961 cgtaacagaa gacaatcgtg tctctcttga tcaaaagctg gtcgatacat tgtctgatag
    34021 cgatcgaatc tatatcgttg cttgcggaac tagttaccat gcggggttag caggtaaaca
    34081 gactttggaa aaattgacac agattcctgt tgaggttcat ttagccagtg aatttggcta
    34141 taacacacct cttctttcaa aaaaaccatt ctttattttc ttgagtcaaa gtggcgaaac
    34201 agcagacagc cgccaagtgc tggtaaaaat caaccgtctc ggctaccctt cacttacgat
    34261 tacaaatgtt gctggttcta ctctttcgcg ggaagcatcc tttacactgc tattgcatgc
    34321 tggacccgaa atcgcagtag catcaacgaa agcttatact gcacaaatag ctgtactaac
    34381 tttattagcc aaagctatcg gtgataaaaa agaatttgaa acttctcttg tattcgatat
    34441 cactcatgaa ttgagtttag ttgctaatgc gatggaatcg gtcatcgccc aaaaagatta
    34501 tttagaagag ttggcagaag tatatctcag cgatacaaga aatgcctttt acattggacg
    34561 tggtgctgac tacaacgttt ctcttgaagc tgctttgaaa ttgaaagaaa tcagttatat
    34621 ccaggcagaa ggttttgccg ctggcgaatt gaaacatgga acgattgctt tgatcgaaga
    34681 aggaacacca gtgatcggaa tcatcagtga agaagtgact ggtgcgcata ctcgaggaaa
    34741 cctgaaagaa gtcgaaagcc gtggcgctaa aacactagtt attgtctcag agggattaga
    34801 aaaggaaagt gaccagctgg tgctaccaac tgtccaccct tatctaacta ctctaaccac
    34861 agtcgtaccg actcaattac ttgcatatta cgcaacgcta ttacggggat tcgacgttga
    34921 caaaccacgt aatttagcga aatcagtgac agtagaataa taaaagaaca aggaccagtg
    34981 tcagacatct gacactggtc cttgttcttt tatt
//
LOCUS       DACDTO010000020        34703 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDTO010000020 DACDTO010000000
VERSION     DACDTO010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34703
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.020"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            155..1861
                     /locus_tag="IWK70_001028"
     CDS             155..1861
                     /locus_tag="IWK70_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9613594.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2019..2921)
                     /locus_tag="IWK70_001029"
     CDS             complement(2019..2921)
                     /locus_tag="IWK70_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9613595.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2918..3622)
                     /locus_tag="IWK70_001030"
     CDS             complement(2918..3622)
                     /locus_tag="IWK70_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9613596.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3653..4294)
                     /locus_tag="IWK70_001031"
     CDS             complement(3653..4294)
                     /locus_tag="IWK70_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9613597.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4332..4601)
                     /locus_tag="IWK70_001032"
     CDS             complement(4332..4601)
                     /locus_tag="IWK70_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613598.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            complement(4574..5263)
                     /locus_tag="IWK70_001033"
     CDS             complement(4574..5263)
                     /locus_tag="IWK70_001033"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9613599.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="IWK70_001034"
     CDS             complement(5270..5986)
                     /gene="pyrF"
                     /locus_tag="IWK70_001034"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9613600.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5983..6915)
                     /locus_tag="IWK70_001035"
     CDS             complement(5983..6915)
                     /locus_tag="IWK70_001035"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9613601.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6912..7688)
                     /locus_tag="IWK70_001036"
     CDS             complement(6912..7688)
                     /locus_tag="IWK70_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9613602.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7742..10927)
                     /gene="carB"
                     /locus_tag="IWK70_001037"
     CDS             complement(7742..10927)
                     /gene="carB"
                     /locus_tag="IWK70_001037"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9613603.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10932..12008)
                     /gene="carA"
                     /locus_tag="IWK70_001038"
     CDS             complement(10932..12008)
                     /gene="carA"
                     /locus_tag="IWK70_001038"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9613604.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12013..13293)
                     /locus_tag="IWK70_001039"
     CDS             complement(12013..13293)
                     /locus_tag="IWK70_001039"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9613605.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13312..14238)
                     /locus_tag="IWK70_001040"
     CDS             complement(13312..14238)
                     /locus_tag="IWK70_001040"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9613606.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14251..15534)
                     /locus_tag="IWK70_001041"
     CDS             complement(14251..15534)
                     /locus_tag="IWK70_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9613607.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="IWK70_001042"
     CDS             complement(15548..16087)
                     /gene="pyrR"
                     /locus_tag="IWK70_001042"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9613608.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16322..17233)
                     /locus_tag="IWK70_001043"
     CDS             complement(16322..17233)
                     /locus_tag="IWK70_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9613609.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17230..17694)
                     /locus_tag="IWK70_001044"
     CDS             complement(17230..17694)
                     /locus_tag="IWK70_001044"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9613610.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17699..18397)
                     /locus_tag="IWK70_001045"
     CDS             complement(17699..18397)
                     /locus_tag="IWK70_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9613611.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18384..18770)
                     /locus_tag="IWK70_001046"
     CDS             complement(18384..18770)
                     /locus_tag="IWK70_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613612.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18917..20587)
                     /locus_tag="IWK70_001047"
     CDS             complement(18917..20587)
                     /locus_tag="IWK70_001047"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9613613.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20770..21003
                     /locus_tag="IWK70_001048"
     CDS             20770..21003
                     /locus_tag="IWK70_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9613614.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21102..21533)
                     /locus_tag="IWK70_001049"
     CDS             complement(21102..21533)
                     /locus_tag="IWK70_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9613615.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21612..22025
                     /locus_tag="IWK70_001050"
     CDS             21612..22025
                     /locus_tag="IWK70_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9613616.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22063..23211)
                     /locus_tag="IWK70_001051"
     CDS             complement(22063..23211)
                     /locus_tag="IWK70_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9613617.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23333..23929)
                     /locus_tag="IWK70_001052"
     CDS             complement(23333..23929)
                     /locus_tag="IWK70_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9613618.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="IWK70_001053"
     CDS             complement(24129..26771)
                     /gene="alaS"
                     /locus_tag="IWK70_001053"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9613619.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27159..27590
                     /locus_tag="IWK70_001054"
     CDS             27159..27590
                     /locus_tag="IWK70_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9613620.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27635..28984)
                     /locus_tag="IWK70_001055"
     CDS             complement(27635..28984)
                     /locus_tag="IWK70_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9613621.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29054..30217)
                     /locus_tag="IWK70_001056"
     CDS             complement(29054..30217)
                     /locus_tag="IWK70_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613622.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30346..30669
                     /locus_tag="IWK70_001057"
     CDS             30346..30669
                     /locus_tag="IWK70_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613623.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30852..32201)
                     /locus_tag="IWK70_001058"
     CDS             complement(30852..32201)
                     /locus_tag="IWK70_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9613624.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32270..33223)
                     /locus_tag="IWK70_001059"
     CDS             complement(32270..33223)
                     /locus_tag="IWK70_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9613625.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33325..34515)
                     /locus_tag="IWK70_001060"
     CDS             complement(33325..34515)
                     /locus_tag="IWK70_001060"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9613626.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         9860 a         7599 c         6091 g        11153 t
ORIGIN      
        1 cctaaaaatt tggatttcaa cgatagaaac actgtttttt atgtaattag tccaagattt
       61 ttctagactt ttgctttact attcggccaa attggttttt cttctttttt tcgttataat
      121 gaatatactt atgaaaagaa atgaggaatg aaccatgtcc tttgatggtg tatttaccca
      181 cgcgatggtc aacgaattgc gtgaaacgtt actatctggc cggatctcga aaatccatca
      241 gccttatgaa aatgaaatcg tgctggtgat tcgttcacgc ggcaaaaacc atcgattatt
      301 attatctgcg cacccaagtt atgctcgtgt acagattacc caaatcgatt atcagaatcc
      361 tgataatccg cctaacttcg tgatgatgct tcgaaaatat ttggatggcg ctattttaga
      421 agacatcgaa caaatcgaaa atgatcgagt gatccatttc cactttgcta aacgaaacga
      481 gttgggtgat ctacaaaata tcatattgat cgttgaatta atgggacgtc atagtacgat
      541 cgtcttagtt aatcgagaaa ctggaaaaat actggatgcg atcaaacaca ttggaagctc
      601 tcaaaacact tatcgttctt tgcttcctgg tgtagaatat gttgctccgc caaaacaaga
      661 ggtcttgaac ccatttagct ccgagaaaga aaaaatcttt cagcggcttt cccaaactga
      721 actagatcca aaagcaatcc aacgtcagtt tcagggaatc ggtttcgata tcgctcaaga
      781 gctgaccaaa agattactgg aacgtcctaa tgaaaagatg gttgtatggg acgaattttt
      841 ctcagccatc agccatcagc ctattcctac tttttacgaa acggaaaata aagatttttt
      901 cacaccgatt gcttatcagg tattgtccga acaggcatct gctgtaacag cttatccaac
      961 actcagtcaa ttattagata gctattatca cgaaaaagca gagaaagatc gggcaaaaca
     1021 acaaggcgga gaactgattc gaaaaatcga aaatgaacta aaacgaaata aaaataaatt
     1081 gaaaaaacgt gaacaaacac tcaaagaatc cgaaaacgct gagaattacc gtagaaatgg
     1141 cgaactactt actacttttc tgacacaagt accacgcggc gcaaaagaag ttgttttacc
     1201 taattattat gaagaagatc ggccaatcaa aatagcacta gatccggcat tgacaccaaa
     1261 tcaaaatgca cagaaatatt tccatcgata ccaaaagctg aagaacgctg ttaagctgat
     1321 tggcgaacaa atccaagaag ccaaagatga aatccaatat cttgaatctg tcttatctca
     1381 actggaaatt gccggcccaa tggatatcga agcaattaaa gaagaattga cttcagaagg
     1441 atacctgaaa aagaagaact taaaaaaaca aaaacgtaag aaaccatctc agcctgatca
     1501 atacttttct tctgacggaa cgctcattct tgttggtaaa aacaatcttc aaaatgacca
     1561 gctgacaatg aagacagcta aaaaaaccga ctattggctt catgcaaaaa atatacccgg
     1621 ttcgcacgtc atcattaaaa gtgacaagcc ctcagatgaa acaattacag aagctgccga
     1681 gttagctgcc tatttttcaa aataccgata ctctgcccaa gtgccggttg atcttgtaca
     1741 ggtaaagcat atccgaaaac caaatggcgc caagcctggt tatgttattt atgaaaacca
     1801 aaagacgatt atcgtgacac cagaagaaga aaaaattaca gctatgaaac aaaacaggta
     1861 atcaatgagt gtttctagaa tcatatttgc gtactttccc acaacagcaa ttagttataa
     1921 attgtttgct acaaaaagag gagttgtgac aaaattctgt cacaacccct cttttttgat
     1981 caacggtgtt caataaacag cgttctcatc tgcttttctt agctctgctg gtccagtaac
     2041 cgagtaagct ctttgataaa tctttgagcg atttcactga gcggacgttt cgccgaataa
     2101 atgatataaa attcatttag ttcgccttct gccaaaggga ttaaccgcaa gtgctcgcga
     2161 atccctccaa tcacaatacc agatcctgaa gcgtaagcat cagtttcaag aacgatattc
     2221 atcaaagtac ctcgatcact cgtataaatc actgtttctt gatcagggat ctcgataagg
     2281 tcttcataaa aatacgggaa attatttcca tcctgtgtaa aacgaacttg aggataagat
     2341 aacaattctt ctttggtgat ctctgcgtgt tcagctaatg gatgtccttg ccttaaaaaa
     2401 atatgcgttt tgaattttcc aatgctgcgg ccacttagct ctccttgttg taaatatctt
     2461 ctgataccag cttgattatg ctcattcata taaataatgc cgatctcact gtgctgctct
     2521 ttgacttctt cgatcactct cgctgttgtc gtctccatga tccgaaattg tttgatctcg
     2581 tctttgaatt cctcgagtag ctgtgcggtc aaaggaccta agaaatcata atgctgggca
     2641 gcgatcgaaa aattgtctct cttttcactc gttagatagt gagtttctaa tgtatcaagc
     2701 tggaccaata ttttctcggc acgatttaag aagtcttgcc cttcttctgt taaatatgcc
     2761 cctcgattcg ttcgagtaaa cagcgtgatc cctaactcgg tttctaattc tttgatacca
     2821 gctgaaagac ttggctggga gacaaacatt ttctttgccg cttctctgaa actaccattg
     2881 tttgctacag cgaccacgta tttcatctgc tgaatattca ttgctcaaac tcctcgaaat
     2941 aataaatttc tcttccattc aagggctgga ttggtcggtt gttcggtcga gcatactgac
     3001 catcgaaggg gagaagaaac tcttggttca gttttcctac atgatccatt acaaaccatt
     3061 ggattggccc gctggtaggc ggagttgtca atgagccaat ataatggaaa tggctttttt
     3121 gctccggaaa gaacacatcc ggatttaccc aatgctcatg tttttctaag ttccaacttt
     3181 ccccattttc ttgattatac agccaacctt cctgtaccat tagctggtac atgacaccca
     3241 caactagatt ctcttttttg ctgttcatat gaacaaaatg aaattcaata tcttcctgtt
     3301 ttccgttgat gatatgttca gaaggcaagt ggaaatgaat atctgtcaaa taatagcgtt
     3361 ctttcttgaa ctctacataa ctgagttggt caaaaggtac taaatgaatc gtgtttttga
     3421 attccttgtc agtaaaccgt tgcttttgat aatgaaagga aagcgtgtct ccttctattt
     3481 tggtttcttc ccccttggtc aaatgaatag gcgactgcaa tggaaattcc gcacctctcg
     3541 caaaccaatc acataaactc gcccaatttt ccggaccttt gtctccagaa tagccccatt
     3601 cgacatccat attctttttc aactatactt ccccctgcat ctcatatcat ttttattttt
     3661 cttctttctt tacaatcgtt gagacggttt ctaaaaacgc ctcttttcca taaaattcct
     3721 ggttcaatag ttccggaaat atctctttaa agaaatactg aacagaaggc cgatgttcaa
     3781 agcttttaat cgtagtcact gcctcaatga acatctcctt ataaactttt tccgggttat
     3841 ttttgatgat ttcatcaaaa gattcataaa gaaggtcaac cttatctgaa atggctaata
     3901 gttctccttc tacggtttca tcttttcctt cgaacaaccg acggcgataa acttcttgga
     3961 actcagctgg aatctcttgg ttgatgaatt cgtccgtcat cttttcttca actgtttgca
     4021 gcatccctct taactctttg cttgcgtatt tcaccggcgt tttgatatca ccaataaaac
     4081 gctctgtgta gtcatgattc aatgattttt catagagcag tttccaatcg atcggttgtc
     4141 cattcgcttc ttcaatgtct cctagaactt gcgcaataga tgctacacga tacgaatgtg
     4201 ctgcaactgt atgctctgta tatttaaaaa atccaggtgc ccttgtgatc gtctctaaat
     4261 tgttcaatcc taaaataaat tcattcaacc ccataaaagt tacctccctt tgagatttct
     4321 ttttccgaat atcatatcat taaataactg atattcgcaa catttccgct ctttaccatt
     4381 tttgaaacag ctcccttacc aaaaaataga gaaaatctat atataataga aaaaagagaa
     4441 gagattattc tcttctttct gctaaccagt cttagaaaaa tgaattttaa aaaaatcaaa
     4501 aactgatcac gatagaagtt tcttttcata cctgatgaaa agatgcttct cgcaatcagt
     4561 ttttggattt ttgctattcc aattgatgat tctggagaca caaatcggat ttctctatct
     4621 tcatcaagcg cgcttcaatc tgccaatttt caggatcttt tttccattct tttagcagag
     4681 ttaattcttg ctcatcgata taacgatctt ctaaagctgc ttcaatcaat acagaataat
     4741 tggtcaacgt cataagaggg atttcggctt tttcgaaatt tgcttttcct tttggcaact
     4801 catacgtaaa gatagccgca acaccaagga tatccgcacc ttctcttttt gctgcttcag
     4861 ctgcttccaa gacacttccg cctgtagaaa tcaaatcctc gatcacaacc atcttctgac
     4921 cttcaaatat acgtccttcg atttggttac ctttcccatg atctttcgcc ttacttcgaa
     4981 tatagaccat tggcaaatcc agtatttccg ctacccatgc ggcatgaggg attcctgctg
     5041 ttgcagttcc agcaatcact tgtacttcag ggtaagcttc tttgattttg cttgctaatc
     5101 cttttgcgat ctcttttcgg acttttggat agctcatcgt gattcggtta tcacagtaaa
     5161 ttgggctttt gattccactt gcccatgtaa aaggattact tggatttaaa aatactgctt
     5221 caatttctaa taagtctttt gcgatacttt gttcaacatt cattttaggt tagctccatt
     5281 ctgtttgaat cgtttgatat gctttataag gattagttga ctgagtgatt ggtcgaccta
     5341 taacgataaa agttgagccg atcttttttg cctgagaagg tgtgactact cttgtctgat
     5401 ctcctgcgac tgttccttct ggtcttatac ccggagttaa gcaaacaaat tcgtctcctg
     5461 ccatttcttt gatcgcttct acttcccaag cagaagaaac cacaccatct aatcctgctt
     5521 ttttggcaca gctagcataa tggatcacac ttttttctaa agaaacttcg attagttgat
     5581 cggcatgcat ttcttcttca cttgtagaag taagctgagt gatagctaga agtagcggac
     5641 gttttccacc ttctggagtc ccctcttcta atcctcgcat tgccgcctcc atcatacgaa
     5701 taccgccagc ggcatgaaca caggtgagat caacaccaag ctttgccaat ccccgcattg
     5761 ctttttcgac cgtattcgga atatcgtgaa gtttcagatc taaaaataca ttatgaccag
     5821 cctcttttag ataacgtacg atttcaggtc cttcttggta gaacaattcc attccgattt
     5881 tcacaaacaa tttttcttct ttcgggaaaa atcgaagaaa gtcttctacc tcttgcctag
     5941 ttgaaaaatc taatgcaata attggtcgtt ggtctagttg attcatcgtt cacgttcctc
     6001 tctcacttct ttgattaatc tttccagcga ttcgatgcct agttcgctca ttcgcacagg
     6061 gagctcttta attagtttag gacagatata cggatcggtg aaattcgcgg tccctatagc
     6121 tacagcactt gctccagcca taaacatctc aagaacatca tccactgtct gcacgccgcc
     6181 catcccaatg attggaaggc tggatatact tgctacttgg tgaatcaaac gaatagcgac
     6241 aggcttgatt gctggtccgg agagtccacc tgtttgattg gctaaaatcg gttttcttgt
     6301 cttaaggtca atccgcatac caagaagagt attgatcatc gtaaacccat ctgcccctcc
     6361 agcttcgatt gcctgagcga ttggaacgat gtctgttaca tttggggaca atttgacata
     6421 gacaggaaca gaagaaactt tctttactgc ttgtgttaat tgaaaagcaa catcagaatc
     6481 tgttccaaaa gcaattccgc catgtttgac atttgggcaa gaaatgttca gctcgattgc
     6541 tttcacattt ggtgcttcgc caattttgct acaaacttct acatagtctt cttcacaagc
     6601 accagcaaca tttgcgatga tgggtaactc attgtactcc tctaattctg gaagaagttt
     6661 atgcataaca acgtctagac caggattttg caaaccaatt gcattcaaca tgccagatgg
     6721 tgtttccgcc actcttggcg tgggattgcc aaagcgggca tttggagtcg ttgccttgac
     6781 catgatcgat ccaagctgat tcaaatcata gtattttccg tattctttgc caaaaccgaa
     6841 acagccgctt gccggcatga taggattttt taattctagg ccaggaagtt tgaccgctaa
     6901 aggattctca ttcatataat tacctctcct attttaaaaa tcggtccttc gtcacaaact
     6961 ttcacacttt ttgttcctgt ttcatcttcc ggcgtatgac aaacgcaagc atagcaagcc
     7021 cctatcccac atgccatgcg ggattctaat gataactgga cattttgatg cgaagaaaac
     7081 agctgttcca ctgtcttcaa taacccgttt gaaccacatg caaaaacagc tgtaggttcc
     7141 gtcttcgttc ctaacaataa attaccgaca tttccgtgca taccgtatgt tccatcgtca
     7201 gtagcgattc ttgtttctcc tagacgatta aattcttttt cataataaac gacttcatgt
     7261 gtggcaaaac ctaaaaaatg cgttggctta attccttttt cgactaactg ttttgataac
     7321 tcatatagag gagggacacc gattcctcca ccgattataa atacttcgtc tcctgtatga
     7381 agattggaca aatcaaaacc attgcctaaa ggacccatga catctaaatg gtctccggca
     7441 gaaagagtag agaagactct agtcccctca ccttccaccc gataaatgat acggcaagta
     7501 tttgtttcgt ggtcgatatg attcaagcta atcggtcggc gtaatagcaa gtctgcacgc
     7561 ggcacgagaa tatgcaagaa ctgtcctggt tgctgcattt cttttactag atcacctttc
     7621 aatgtcattt caaaaatctt tggtgccagc tgtttttgct gaacgatttc catcatttct
     7681 tgtttcatct gtcccctcct gcaacggttt ttcgtctttt tgtcttaaca tgtgctcgcc
     7741 gttaaatagg gttagttgaa aaagcacggg attccatcac tcgaacgatc gcatctgctg
     7801 tgtctaaaga agtaaacaat ggaataccat gttcaactgc ttctcttcgg atgatgaaac
     7861 cgtctttaga agcatttccc cgatctttat caatcgtatt gacaaccact tgtgttttac
     7921 catctcttat taagtcgact acattttttt ctgctgcttc ctcagaaatt ttcgctactg
     7981 tttcaacgac cagaccattt ttttcgaaat aagcagctgt atgtttcgtt cctatcagac
     8041 tatagccaat ttctgtaaaa cgtttagcta attctagtgc ttcttctttt gtttcgtccg
     8101 caatcgtaaa taatacggct ccatattcag gcaagtgtaa tccagaagct tcgaatgcct
     8161 tgtacaaagc tttgtctaat tgtctgtcag atcccattac ttctccagtc gatttcattt
     8221 caggccctaa gtaagtatca actttctgca atttcgtaaa agaaaagaca ggtgctttaa
     8281 catgaacttc ttttgtttct ggataaagtc cgttttcata accaagtgtc tttagtttct
     8341 ctcccaaaat agctttcgtc gcaatttgtg ccattggaat tcctgtcacc ttacttaaga
     8401 atggaacagt ccgacttgct cgaggattga cttcgatcac ataaacctca ttttcatgga
     8461 tgacaaactg gatattcatc atcccgacac aattcaaacc taatgctaag cggatcgtgt
     8521 aatcttcgat cgttttcttg atcgattcag ataatgtttg aggtggatac acagccattg
     8581 aatcccctga atggacacca gcacgctcaa tatgctccat gatccctggg atgagtactg
     8641 tttcaccgtc acaaatcgca tccacttcgc attcaccacc aagcaagtaa cggtccacta
     8701 gtactggatg ttctggagat gctttcacag catgttccat gtaatcttct aaatctcgct
     8761 ggttttcaac gatttccatc gcacgtccgc ctaatacata gcttggacga acaagaactg
     8821 gataaccaat tctcgaagcg ataaccacgg cttcttcttt acttgtagca gtatccccta
     8881 aaggttgagg aatatccaac tctttcagcg cttgctcgaa taaatcacga ttttccgcac
     8941 gatccaagtc ttcgatcgtt gtccctaaga tttttacacc agcagcagtc aaaggttcgg
     9001 ctaagttgat tgctgtctga ccaccaaatt gaacgattac tcctatcggc tgttctagct
     9061 cgatcacatt catcacatct tcgaaggtca atggctcaaa atataattta tccgaaatag
     9121 aaaagtcagt cgaaactgtt tcaggattgc tgttcatgat gattgcttcg tatcctgcct
     9181 gctggattgc tttgacagaa tgcacagtcg catagtcaaa ttcaacccct tgtccgatac
     9241 ggataggacc agatcccagt actagaattg agggcttttc tgatcgtgaa ctttcatttt
     9301 catattcata ggaactataa aaataaggtg tttttgattc gaattccgct gcacaagtgt
     9361 caaccatctt atatacagga atcaaatcat tttcttttct catctggcga acggcttctt
     9421 cgtttatttc ccacagctcc gccacttttt tgtctgtaaa accgttttgt ttcgctgttt
     9481 tcaatatctt gtcatccaac ggctgctgtt tcaactgctg ttcgatttct acgatatgca
     9541 acagtttatc taaaaagaat aaatcgattt ttgtcaaatc cgctaattct tctatggtgt
     9601 accctcttct aatagcttca gctacataaa acaggcgatc atcttgtgct cgaaccactt
     9661 tttggattag gtcatcgtcc gtgatgtctg ccaactcagg aagttcgata tggtgtgtac
     9721 cgatttctaa agaacgcaca gctttcaata aagactcttc gatattccga ccgatagcca
     9781 tcacttcacc agttgctttc atctgcgtgc ctaaaagccg ttctccgttt tcaaacttat
     9841 caaacggcca gcgaggaatt ttagctacaa cataatctaa tgcaggttca aattctgcaa
     9901 aagtcgttcc tgtaacagga tttttcattt cgtctaatgt taatccaagc gctattttag
     9961 cagctagctt agcgatcgga tagccagtcg ccttacttgc taacgcagat gaacgagaaa
    10021 cacgcggatt gacttcaatc acataataat taaaactatg tggatcaaga gcgagttgga
    10081 cattacagcc tccctcgatc ttaagtgctc gaataatcga aagagaagca tcacgaagca
    10141 tttgatattc atggtcagac aatgtttgac taggggcaaa tacgatagaa tcacctgtat
    10201 ggatacccac cgggtcaaaa ttttccatat tgcagacaac gattgcatta tcagccgaat
    10261 ctctcatcac ttcatattca atttctttaa aacctgcaat acttcgttca atcaaacatt
    10321 gggtagcggg ggaaagactt aatccatttt ctgatacgat acgtaattct tcttcatttt
    10381 cacacatacc tccaccagtt ccgcctaaag taaaggctgg acgaacgatg acaggatagc
    10441 cgattttatt tgcaaaatct acagcttctt ctactgtatt gacgatttca gactcgggga
    10501 ttggctgatt caattctttc atcaactgtt tgaatagatc acgatcttct gcttgatcga
    10561 tcgcagaaag ctttgttcct agaagttcta tgttcaattc ttctaaaata cctgatgctg
    10621 ccagctccat tgccatattg aggcctgtct gtccgcctaa agttggaagg agagcatctg
    10681 gacgctcttt tcgaagaatc cttgatacaa attcaagtgt gatcggttcg atatatactt
    10741 gatctgccac ttctttatcc gtcatgatcg tcgcaggatt agaatttact aaaaccactt
    10801 catatccttc ttcttttaag gcaaggcagg cttgtgttcc tgcgtagtca aattctgcgg
    10861 cttgaccaat gatgatcgga ccagatccaa tcaccataat tttcttgata tctgtacgtt
    10921 tcggcatctg tttactgctc ctttcctgcg tccatcattt ccataaattc atcaaataaa
    10981 tgtagtgcat catgcggacc aggggcagca tctggatgaa actgcacact gaaagcggga
    11041 taatctctgt gtttgactcc ttctactgtt ccgtcattta cttctacatg agttacgatg
    11101 agtttttctg gatcaatcgt ggcttcatcg actgcatagc catgattttg agaagtaaaa
    11161 tcaattcttc cggtcgctac ttctcgtacc gggtgattta atcctcgatg tccgaatttc
    11221 attttatagg tatctgcacc gttggctaaa gcaaataact ggtgaccgag acaaatacca
    11281 aaaatcggta cttttccttg gatttttttg atcatttcga ttgcttctgg cacatctttt
    11341 ggatcaccag gtccattcgt cagcatgact ccgtctggtg atagcgctaa aatcgtctcg
    11401 gcatctgtgt tatagggaag tactgtgagg ttacagtttc ttttactcaa ttctcgtaaa
    11461 atactatgtt tcaagccgaa gtcaatgaca atgactttcc gacctgttcc tggacttgga
    11521 taaggtttgt tagttgagac ttgctgcact tgattggttg ctagaacggt tgctttcagc
    11581 tgatcaaatg catgttcgaa ggaatctcca gcatcaacta tactagcttt catcgttccg
    11641 acttcacgga ttttgcgagt aagggcgcgc gtatcgatac ctgatattcc aggaattcct
    11701 ttttgtttca aaaatgaatc tagcgtcatt tggtttcgcc agtttgaagc tacacgtgca
    11761 tgttctttta ctactactcc tttgcatgtt ggagaaatcg attcataatc atctctattt
    11821 accccataat tacccaccat aggataggta aatgtaatga tttgaccgtt aaaactttga
    11881 tcagtgatag tttcttgata accagtcata cttgttgtaa aaacgatttc tccgaagaca
    11941 ctagcatctg caccaaaagc ctttccttcg aaaactgttc catcttctaa tataagcaaa
    12001 cgttccatac ttttattctc ccttctgcca cacaagtttt ccgttgacaa aagtcatttg
    12061 tgtttcgcct tttactttcc agccgataaa aggtgtattc tccccttttg ataaaaagtc
    12121 ttctttatcg atcgtgcaag tttgtgtaat atcaaaaact gctacatcag cgggggcacc
    12181 aaccgttagc gttcctgcat tcaaaccaaa aatctcagcc ggtttaactg ccaaccaatt
    12241 gatcacttgt tccaacgtga atcgaccagt ttctacaaaa tgagtataga tcagttgaaa
    12301 cgctgtttcg cttcctacga taccaaatgg tgatttcatg aaactttggc ttttttcttc
    12361 taatccatgg ggggcatggt ctgtagcgat gcaatcgatc gtaccgtcaa gcaagccttc
    12421 gatcaaagct tctctgtcct cccgtcctct tagcggcggg ttcattttcc aaaagccgaa
    12481 atcttcggga atatcttcgt cgatcaaaat aagatgatgg ggtgaaactt ctgctgttac
    12541 gtggattcct gctttttttg cgtctctgat tacacggaca ctttctttag tagaaacatg
    12601 gcaaacatgg taatgaacac cagttgcttc agctagcaac agatctctag caatttgaga
    12661 agactcggtt acgcttaaga tcccgggaag accaagttct tctgccttct tgccagcgtg
    12721 cataacgccg ccaaataata aagattcatc ttctgtatgg gctactaggg ctttattgtt
    12781 ttttgcagct tctttcatag ccagatacat cgtcccagct gtttggacac ctacaccgtc
    12841 atttgtgaaa gcaaaagcac cttcttcaat cagtgcttct tgatcaacta atttctcact
    12901 tcgcagattt tctgtgattg gcgcatactg caataccttc acaactgcat cttttctaat
    12961 gatgtcatat acttgtctca gcttttctgc cgtatcaggt acaggattta aattcggcat
    13021 cgcgcataca gtagtgaaac cgccacgagc agctgagcga gtcccagctt cgatcgtctc
    13081 cttgtatgta aatcctggct ctctcaaatg aacatggacg tccactaaac caggtgtgat
    13141 caattgtccc tttgcatcaa acacttcatc aaattcggct tctgagaatc ctgtaccgat
    13201 tgctttgatt cgcccatctt ctatccaaat ttctgctggt gtcgtcatat tttttcgtgt
    13261 attgatctga ccgtttttaa tgagtgtttt cattttttca tcctcctata attacgcttt
    13321 tccatttagc actgcttcta agattgccat tctgacgaat actccattgc tcatttgctg
    13381 aacgatccgc gactgtaatc cttccactaa tgaatcagct aattctacat cacggttaac
    13441 tggtgctggg tgcataatga ttgcatgttt ttgcaacttc ttcgcacgct caactgttaa
    13501 cccatgttcc tgatggtagc cttcttttga gaaactttct tttccatcat ggcgttcgtg
    13561 ttgcacccga agcatcatca tgacatcaac ttgatctagt agttcatcca atggcatata
    13621 atgtccgtac acttcgaatt catcgtcgta ccattctcta ggacctgaga agaatacttg
    13681 tgcgcctaga cgttttaaca tctgcatatt ggatttagct acgcgagagt gagtgatatc
    13741 acctacgatg gcaactttca gaccttcaaa atgaccaaat tcttcataaa tggtcatcaa
    13801 atcaagcaag cattgggttg gatgctgtcc gcttccgtct cctccattga taatcgaaca
    13861 ctggattgtt ttactttgta tcagttcgtc ataatagttc tcatcaccat gacggataac
    13921 cgctacgtct acgcctaatg ctgacatcgt cagtactgta tcgtatagtg tttccccttt
    13981 ttggacagag cttgtgcttg cttcaaattc aatcacttct agccctagtt ttttctccgc
    14041 tacatcgaag cttttatgtg tgcgagtact attttcgaaa aagaggtttg tagcaaagta
    14101 ctggcgcttt tgcggagtcc aattagcacc ttgcttgaat tcctgccctc tgcggatcaa
    14161 gcccataact tcttgatcag taagtgcctc aacggtcaat aaatgcttta agctgatacg
    14221 ttctgatttg atgatcataa tgtttcctcc ttagttttct tcgcttcgtg aagtttctgg
    14281 taagataaga tttaaaatga tgccaagtac agttgctaat gccattgccg acaatgtaaa
    14341 tgtgcctact tcaaagacga gtccgccaat tcctactact aagataactg aagcaatcaa
    14401 cagatttttc tttttatcaa aatcgatttt gttctcgatt aaaatcttca ttccacttgc
    14461 ggcaattact ccaaataaga taaaactgat tcctgagatg actggtccag ggatacttaa
    14521 aatcaaagcg cttaattttc ctacgaatcc taaaatgact gcaaatactg cagctccacc
    14581 gatcacaaac acgctatgga cacgtgtgat agccagaaca ccaatgtttt caccgtaact
    14641 tgttacagga ggacctccga caaaggctgc cacgatttgt gctaatccat cacctgtcaa
    14701 cgtcttatga agacccggtt cttcaaagaa attacgttta gttagtttat tcaataccat
    14761 aagatggccg atatgctctg tcatcgtcac aaaagcgatg ggtgccattg ttgtgattgc
    14821 tccaagatat agctttggtt catattgtac aaagggaatc tcaaagttag gaacagcaaa
    14881 ccaggcagcg tctacgattg gcttagtatc cacgattcca aacagcaagg ctacgatgta
    14941 tccgctaata atacctagta aaatcggaat caatccgaga aaacctctta gccacatgtt
    15001 gaagaaaatt gttaatccta atgtgatcag cgctacagca atatacttga aatcatacgt
    15061 gccctgatta tacatagcat ttgttgctgc atttgccgca agtcccaacc cgataaccat
    15121 gatgactgga ccgacgacga ttggaggcaa gattttatcc agccatgctg aaccgatctt
    15181 tttgatgatc agcgcaacaa tcaaatagac taatccagtt gtgactgctc cttgtgcaat
    15241 cgctggataa ccatctgttt tcatcaaaag ctgcatcgct gcaatgaaag cgaagctgct
    15301 tcctaagtac gcaggtatct ttcctttagt agtaaataag tacaccaatg tacctagtcc
    15361 tgaactgact agcgcaatac ctggatcgat acctactaag atggggacta acacggttgc
    15421 tccgaacatc gtgaacaagt gttgtaaact taatcctacc caatgtcctg gggttgggcg
    15481 atcgtggata tctaacacca cgtcatcatt atgaaatcct tttttctccg acatgtttct
    15541 ctctcctcta atcttcctct ttcaaaatca tgatgcgatc ttgtccatca agctcttgca
    15601 tctctactaa aatctcttcc gcccgagatg ttggaatgtt cttcccaaca taatctgcac
    15661 ggattggcaa ttctcgatgc ccacgatcga ccaggactgc taatgaaatt tttcttggtc
    15721 tgccaaaatc cattactgcg tccattgctg ctcgaatcgt tcgtcctgta tacaaaacat
    15781 catcaatcaa aataacttct tttccttcta aggaaactgg gatgtcagaa gaatgaagtt
    15841 ccggttcttc tggattttct tttttgtcat ctcggtataa agtgatatcc agttctccta
    15901 caggaatatc gatatcttct agctgcttca ggcgttcggc aattcttgat gcaatgtaga
    15961 ttcctcgtgt tttgatacct accaatacga tatcttggat actatggttt ctctcgataa
    16021 tctcataagt aattcgagtc aatgctcgct tcatggttac ctgatctact acttcttttg
    16081 cttgcatttt cattccccct acttttttgc ataaaaaaac tcctgcccta agaggacagg
    16141 aggaatgttt agactgagcg tatactgata cactgatagc tatactatgt caatgagcaa
    16201 tctacttatg gcgcaccata cgtatccttg ctcccaccct tcttagcctc acgggactaa
    16261 acttaaagga attctattaa gttttatgct ttcgttgtta attactatac cgttatctaa
    16321 attaaaatgc aatgtttttt cttaattttt ctaaagtttt ttcaaaaact tctggaagag
    16381 gtgcttcaaa gatcatcttt tcgtttgtag tcgggtgcgt aaaacctaac atttgcgcat
    16441 ggagaaactg accattgcct ttcaaagttt tcttcggtcc gtatactgga tctccagcta
    16501 acggataacc gatatacttc atatgcacac ggatctggtg tgttcttccg gtttctagtt
    16561 ttaattcaac cagagtaaat ccttcgaaac gttccaatac agtaaaatgt gtgacagccg
    16621 acttcccatc ttctcttatc gcctgcattt tccggttttc cttcgaacgg ccgattggcg
    16681 cattgatggt tcctttttcg tgaggaatct ctccatggac taaagcaaca tatttgcgca
    16741 aagaggtctt atcttttaat tgctgagcta atgattcgtg tgcctggtca tttttggcaa
    16801 ccattagcag tccggaagta tctttgtcga ttcggtgaac aatacctgga cgaataacgc
    16861 cgttgatcgt agaaagatcc ttgatatggt acatcagcgc attgaccatt gtgccatgcg
    16921 tatgacctgc tgaaggatga acgaccattc cttgaggttt gtttatcacc gctacatctt
    16981 catcttcgta aacgatcgtc aacgggatat tttctgggac cacatctaat gtttctggtt
    17041 cggggatcaa gacagtaatc tcgtctcctt gttttacttt gtaatttgct ttgactggct
    17101 gtccattgac agaaaccgct ccatttttca gccacagctg aatttgcgaa cgactgtaat
    17161 cggtgaataa attagtcaat actttatcga tcctaccaga ttcatggtga atcgtcgttt
    17221 taagttctgt catttttttc tttgatcctt ttcatctaaa ataatataaa ttaacataca
    17281 aatcactcca catacaagtg tcacatctgc tacgttaaaa atcggaaagc tcataaaatc
    17341 tgtctggaac atatctacta catagccaag acgaatacgg tcgataaaat tgccaatcgc
    17401 accagctaga atcaaactca atccaatcat gaaccagcga ttaccttttt cacgattttt
    17461 aatcatcaat gtgagaatca caacgactgc aatgagtgtc actatagtaa agaaccacat
    17521 cttcccttct agcatactcc atgcggctcc agtgtttcga atatgattca gtgacaaaat
    17581 acctggtata agatttttcg tctctccaag cgctagatta tcagtgatcc accatttgac
    17641 caactgatcc actcctacta gcaaagcgct gaatatccaa taaataatta acaaaaaatc
    17701 aatccttttc tatttgttta taaatatcac gaggtcgtac gatcccgagc agattaccat
    17761 cgggattccc atctttagtg ataagtacgg cttccaaagt cccttgctca cgaaacatcg
    17821 cttcaacctc gtaaatatgt gttttagctg gaacaaaatg ataatttgtg tactttccgt
    17881 tttggatgat caaatccaac gctcgtttat ttgttaaatc aaccggacct ttttggcttt
    17941 cttttgctag ccaaaacccg atattacgta aagtaatcaa tgctacaaat tttcctttat
    18001 tgtataacgg aaattgtgaa tacctttttt ctgctactat ctttaacagt tcaagtaacg
    18061 gaagatcttg ttcaaaacct gttacacgtt ttgcaaatcg aggcagcacg gtttcaggct
    18121 gaatcagttc ttgttcgatc agctggattc gcttgactgt ccaatcattt ggctctgcaa
    18181 tgacaaaatc gactgcgatc cgatcatgga cgatcgcatt ccttaactga gcaagctgta
    18241 aaagatcatc ttcgtatttt ctgatcatta attgttttct ttgagctagg cgtctgacaa
    18301 gttcggtaaa tcccatattt cgtgcatttc ccatctcgtc tctcatccat ttttcgattc
    18361 gattgaaact gattaaaaaa agatcagagt ttgccataat tttcctcctt gatcaaatat
    18421 gcccggtttt ttatatagaa aaagaacgca atcaacacaa taactgcaaa aaaactgaaa
    18481 aataaatttt gtaggaaaac ggcccccaac ccagttgcta gaaataaaaa cggatagatt
    18541 tgaagaatca agtaattcat tgtgatcaca taagcaggct ttgcttcttt cactgtataa
    18601 acggggcttt ttagccaata aaaggtgacc tgcatcgtaa agggcacttc cagatcaagc
    18661 gttatctttt gttcatgact gagaacactg cgtttttcat tgttgattct tagctgaatc
    18721 gagctaccca taccgtaaaa accggttttt cttataaatg tcgttttcat cataccctcc
    18781 tattatgctt tagtatagct caaaatacaa taaagaaaaa gaatgatcct agtgtaaaca
    18841 aaattaattc cctatcgtct aaaaaaagaa gctgtagtca aaatacagct ccttcgatgg
    18901 tagattataa tcaagcttaa aacaatccca acacttctcc gtcatttgtc acgtccatat
    18961 tcaatgcagc aggtttttta ggcaaaccag gcatcgtcat aacatctcca gtcaaggcta
    19021 ctaaaaatcc tgcccctaat tttgggatga tttcgcggat cgtgatggtg aaatcttccg
    19081 gtgcacctag cagtgaagga tcatcagaaa atgaatactg tgtttttgcc atacaaatcg
    19141 gcagtttgtc ccatccattc ttcgtaaact cttctatttg tttttgcgct tttttgctga
    19201 aaacaacacc atttcctccg taaattttct tgacgattgt ttctgtcttt tcttgtatcg
    19261 tttcttctag gcggtataat ggcttgtaat tagcttcttt tctatcaatc aatcgtacta
    19321 cagcttttgc taaatcaacg ccgccttctg ctccatctgc ccatacactc gctctcttgg
    19381 caagaatccc ctgattttca cacaagtgct ccaacagtgt gagttcattg tctgtatctg
    19441 ttacgaattc attgattgcg acgatcacag gtagctgata ttgttccata ttacgtatat
    19501 ggcgctttaa atttgcaaaa cctactttca aagcatcaag attctcattt ttaagttcat
    19561 cttttttcat tccgccatgc attttcaatg cacggacagt ggcgacaatc accacagcat
    19621 ccggcgcttt tttcaagtta ggtactttga tatctaaaaa tttctctccg ccaagatctg
    19681 caccaaatcc tgcctctgtg acaacgtaat ctgctaatct caatgctgtc ttggtagcta
    19741 aaatactatt gcagccatga gcgatattgg caaacggtcc cccgtggaca aaagctggtg
    19801 ttccgtaaat tgtttgtaca agattcggct tgattgcatc tttcagcagt aatgccagag
    19861 cgccttctgc tttcaagtct ccagcagtca caggctgacg gtcaaatgta tagccgacaa
    19921 cgatccttgc aagacgagcc tttaaatctt caaggtctgc cgcaagacac aagatagcca
    19981 tgatttcgct ggcaactgtg atatcaaacc catcttctct tggaacacct tggattggcc
    20041 cacccaagcc aacaattact ttacgcagtt cacgatcatt gagatcgact acccgtttcc
    20101 aaatcactcg gcgggcatcg atgttcaatt catttccttg atgaatatga ttatctaaaa
    20161 gagcagataa tgcattatta gctgttgtga tagcatgcat gtcaccagta aaatgcaagt
    20221 tgatatcttc cataggcaat acctgtgcat atcctccgcc tgcagcacca cctttgattc
    20281 ccatcacagg accaagtgag ggttctcgca atgcaataac agatttttta ttgattttat
    20341 tgagcgcgtc tcctaaccct acagtgatcg ttgactttcc ttcgccagct ggtgttggat
    20401 tgatggaagt aactaagatc aaatgcccct cagcgttatc ctttgatcga ttgattccag
    20461 aaaaattgat tttcgcctta tatttcccat acagttccaa atcgtcttcc ttaagatcta
    20521 atttactagc cacttcaaca atcggttcta gctctgtttc ttgtgaaatt tgcagatcat
    20581 tcttcatttg attacccctt ttcacgaact ttattcaaca aaatatcttc tttgttaaga
    20641 tatgccaatt ataccttttt ttattcagaa aaaaaacata aaattacagg tttttaattt
    20701 atttgacgta cttataaata ctctttataa tgaaggctat gatcagactt tagaaaggtc
    20761 gtgagtgctt tgaccacagg aattgtaaaa tggtttgaca acaaaaaagg atacggcttt
    20821 atcagttatg atgacacaga agaaattttt gtacatttta ctgcgattga agaagaggga
    20881 ttcaaaactt tggaagaaaa tcaagtggtc gaatttgaga tcatcgaagg gaatcgagga
    20941 acacaagctg cccatgtcaa gaaaggcaac gttcagacta ccgatactag tatgcatgaa
    21001 tagataaaca aatagagtga gacgctaaac gggatgaacc gtacgtctca ctcttttctt
    21061 ttcctgtaaa aattcgtttt tttaggttct gatttttaaa attagctttt tctttttttc
    21121 gtataaggat aaggaattga ttctgatttg ggtccgtttt tttcaaaatg atcaaaaagg
    21181 actacttgat ctttttttcg catcatgaaa acagatgggg ctttctcatc ttgcaacatc
    21241 atttgcaccc cagtattagt tggcgtcaaa gatcgaatct gtccgacaga tatttctcgg
    21301 tctttccaaa aagcagcata acgtataatc acagagtttg attttaaaat aaaagaacgc
    21361 cgcaaaccta gaaatagaaa aacaagaaaa atacctaaaa caaaattgct tttccaataa
    21421 ggacctgtgt tttctaaaga aagaatcaga ctaaaaaaca gaatgatcaa ggtcaatgac
    21481 caatagatga tcgtatgtgc cagttcaggc tgccatcgaa tatttttttt cattctctaa
    21541 cctctcaccg attcagtaat gatataatct taccataaca ttagtttgaa caaaaaggag
    21601 aatctctttt tatgttaaaa gtatacattg atgcctctac aaaaggcaac cctggtccta
    21661 gtggaggtgg aatcttgatt gtctatcaag gtaaacaaga acagctatct atcccgcttt
    21721 ctatagggac aaaccatcaa gcagagttcg aagtcttttt aaaaacttta gaatttctaa
    21781 agaaaaacga cttacagaat gaaacgattt tctgtttttc agacagcaaa acactagttt
    21841 ctacggtcga caaaaacgca acgaagaacg agctcttcct tccctacctt caaagaatcc
    21901 aagaacttct atccgaattt tctttattga tcctgcaatg gttgcccgaa aacaaaaata
    21961 aaggagcaga taatcttgca cgtcaggctc tccataaaca attaaaagaa aaaaagactc
    22021 tttaaattaa aaactttaag gagtcttttt atagtttctc atttaatata aaagataatt
    22081 gtgatcaaac gtaatttcca attggtaatc tccgattttt tcttctattt gactacgtaa
    22141 gattctttct agttctgtat caacaaactg gaatttctca gggaccacta catcaaattg
    22201 cagcgttttt ccttgatgaa ttactctaaa atcatgaata ttaagccctg tacctgtttc
    22261 aagaagaatt tgctttaact gccgcaagat attatagtat ttttcgttat caacaggcat
    22321 tggatccaag tgacagacaa gttccacatc caaagacttt ttaaagtctt tttctatcag
    22381 atcaataatc ttatgagcca cattcaaatt cattgattca tctacttcga tgtggactga
    22441 tgcaaagcgt ttattgggtc catagttatg caccaataaa tcatgatagc caagtatatc
    22501 atcgcaagca tttaaatgct cttccatttc ttcaatctct gctggcgttg ggcgacttcc
    22561 taatagttcg tatacaaaat ttcgaagcat cgtcaatcca ctataaagga tatataatgc
    22621 taaagcaaaa cctatatatc catcaatacg ccatccagtt acccattcga ttgctgctga
    22681 aagcaataca gccagtgtag taaagacatc attataacta tctgttgctg tagctctcaa
    22741 tgtttcagaa tcaatggatt gcgcaataac ctgatacatt ttgccttgtg caaatttcaa
    22801 aaagatggaa atgattagga caaaaaagac gatgggtgtt aaagagacat tttctggatg
    22861 gatgattttt tcagcagaag actctaaaaa ttgaaatcct acataagtaa tgatgatcga
    22921 aacaaataac ccactgatat attcgaagcg ttcgtgtcca taaggatgtt cctgatctgc
    22981 tggctttgca gcaattttaa atccgatcaa agtcaatatg gatgaagcag tatctgatag
    23041 actgttcact gcatcagcca taatggagac acttcctgaa aaaagcccga tcattagctt
    23101 agagacaaac aagatcagat tcgtaaccaa tcctaagata cccgctagta ctcctttttt
    23161 tgtccgctgt tgcggtattt gtttcttttt ttcttcttgt ttcttagcca agttttttac
    23221 cccttctagt ttgcttgttg ctcaggtaaa aagatggaga ggacgattgt tttcctctcc
    23281 tctcttcctt tttctatcta tcgtgcgtcc tattactatt aaaagatggt cattattctg
    23341 gtttcaacgg tgttgctgca aataacgctt ttgctagtaa tggtgttatg attcccgcac
    23401 ctattgcttc tggtatccca ttgattgctg caacccctgc taaggtttta aataaaagag
    23461 cagagtctac accataggca tctgccgttg cacctgtata aaataatccc atgaatccta
    23521 acactaagac tgtattgacc aatgaaccca gtacccctgc aaaggctgag ctgatcgtta
    23581 ttgatttatt tttttgataa atccagtgga aaaccaagcc tgctactaag ccaacaagta
    23641 ctctgggaac taccgagata atcggattcg tgaacactaa tgtatcaaat ggcgttgttg
    23701 gcatggcata ggcacgaaca attgtagcga ttccccaaaa caagccaatc accatgccat
    23761 cttttgggcc taatactact gcagcaataa ttactgtgat atggataatt gttaaactaa
    23821 tgaatcctaa tggaataaac cccagccaag gaaccatcgc ttgtacgatg atgattgcta
    23881 acaagatcgc acgtaagact agtcgaaagg tcttatttct actattcatt ttttcacccc
    23941 tttttattta tactaatcgt aaaaatttct gtcactttcg tcaactaaag accatccgaa
    24001 tccgaccatt tgattagatg cgccatccaa ttgaaaaaag tatgagggca agacttgttt
    24061 gtcttcgccc ccataatcat gtttcgttca atgtttgaac ttttgttttt ctgtcaaacg
    24121 taaacgccct attgttgcgc aacccattct ttcgcagctt taagtgcatc tggaatacca
    24181 gcaggatttt tcccgccagc ttgtgccatg tctggacgtc caccaccgcc accgcctact
    24241 ttcggtgcga ttgctttaat caagtcgcca gcttttagtc cagcttcatt tgattctttc
    24301 gtcattgccg ctagcagact gacttttcct tcttgttcgt ttgctaaaac aagaatatct
    24361 gaaatatttt tttgtttcca ttggtcagct aattgacgca gctgattcat atctttcact
    24421 tttacttgtg ctgcaatgat tgtttttcca ttgatcgttt ctggatcttt gaatacatca
    24481 ccagcttgtt gattcgctaa cttgctagct aattgttcat tttccttttg tagctctctt
    24541 acttgctgtt gcaattgttc cgcttttgaa acaacttctt tcaattgagg tgatttaaca
    24601 attgttgcta cttgttttag ttgtttttct tcttcttcaa gtagttcgta cgcttcttta
    24661 cttgttacag cctcgattcg tcgcacgcct gcaccaattc ctgattcaga aacgattttg
    24721 aagattccga tgttttctgt atttgctaca tgtgtgcctc cgcacagttc gattgaccag
    24781 ccgccaatat ttactactcg aacgtcatgt ccgtattttt caccaaacaa agccattgca
    24841 cccatgtttt tggctgtatc gatatctgtt tcaattgttt caactgggat agcttcccag
    24901 attttttcgt tgacgattct ttccatttca gctaattcat ctgaagtgat ttgaccaaag
    24961 tgggtaaagt cgaatcgtaa atgtccagga gcgactaaag aaccagcttg atttgcatgc
    25021 tcgccaagta cttctttcaa tgccttgtgc agtagatggg ttgctgtatg gtttttgatg
    25081 atccgattgc gcatcgattg atcgatcaat agatgatagg tttgtccttc actcattggt
    25141 gcttttactt ctacggtgtg cataaattga ccattaggcg ctttttgaac atctgtaaca
    25201 actgctacga tttcgcctac ttcattcatg atccatcctt gatccgctac ttgcccaccc
    25261 atttctgcat agaaaggtgt ttgatcaaag atcatttgag cttcaccttc tgcgacttca
    25321 ttcaaaagag actcaccttt aacgatgacc aataattgac tatctgattc ttgttctgtg
    25381 tatccaatga atttactttc tactttgata tctgtcaata aagcagattg tacccccatt
    25441 gatttttcag tactgcgggc agagcgagca cgttcgcgtt gtgcatccat ttctttttca
    25501 aaaccagcat gatcaacttt cagtccttca tcttctgcca cctcttccgt taattctacc
    25561 gggaagccat atgtgtcgta tagtttgaag atgtcttttc ctgctagagt cgtcccgttt
    25621 tcgtttttta cttcttggat cacttgattt aagatttcca aaccttcatt gattgtttca
    25681 ttgaaacgtt cttcttctgt acggataact ttttcaataa attctttttg ttgaagtact
    25741 tcaggataat aacttaccat gatttcccca acaacaggga ctaaacgata taagaatgct
    25801 tgttcaatac ctaatttttt cccatgcatg acagcacgac gaagcaaacg gcgtaagaca
    25861 taccctctcc cttcattcga tggcaatgca ccgtcgccaa tagcaaatga aagggcgcga
    25921 atatgatctg cgattacttt gtaagaaata tctgttactg ggttttcacc atattttact
    25981 tgtttgctta gcttttctac ggcgtggata ataggcataa acaagtctgt ctcaaagttc
    26041 gttgacgcat cttgaacgat tgagaccatg cgttccaatc ccatccccgt atcaatgttt
    26101 ttatgaggaa gtggttcaaa tgtgttatca ggcttatggt tgaattctga aaatactaag
    26161 ttccagattt ctaaataacg ttcattttct cctcctggat agttttcagg atcgtcttct
    26221 gctaaatcgt tataagcttc tccacggtca tagaaaattt ccgtatccgg accacatggg
    26281 cctgcaccga tatcccagaa gttgtcttct acatcaataa tgtgatctag tggcaggcca
    26341 acttcttcat gccagatgcg ttttgcttct gtatcttttg gataaactgt tacataaagt
    26401 ttttctggat cgaatgccat ccattttgga tcagtcaaaa attcccatgc ccagtggatg
    26461 gcttcatttt tgaaatagtc accaatcgaa aaattaccca gcatctcaaa catcgtatgg
    26521 tgccgtgctg ttttaccgac attttcgata tcatttgttc gaatagattt ttgggcattt
    26581 gtgattcttg gattttctgg cacaactgaa ccatcaaaat attttttcaa tgttgcaaca
    26641 ccagagttga tccacaataa ggttggatcg ttcacgggga tcaaagacgc actaggttca
    26701 atcgtatgtc ctttagattg gaagaaatca agaaacattt gtcgaacttg tgcactactt
    26761 aattgtttca tgtcattacc tgctttctat aaattaaata caaaaaaaca ccatcacagc
    26821 caaggacgaa aactcgcggt accaccttga ttgcaatgat gtgtaatcat tacctcttaa
    26881 actcgataac gctgagctgc gtcggtattt ctaccaaaat agaaaaaggg agcaatttga
    26941 ttagtgacta ttcttttttc agccaagaaa gaattttctg tgagtcttaa aaaacaaatc
    27001 atatcctttt cgtacttaat tagtatacgc attctattga tcactgtcaa tatctcaatt
    27061 tttattttca tttccttttg gtcatactcc acgatttttg gtacgataag aataaaggaa
    27121 tcaatacatc tctgctattc atgacaagga ggaatatgat gaaaaaaata gttggagcgg
    27181 atgaatggat acgcgcagct agctgctttt tgagatatca agtttttgta ttagaacaag
    27241 ggattcctgc agaactagaa ttcgacggaa aagatatccc gcaacaaacc tattatctat
    27301 ttcttgatca aaaaagacca gtagctactt tgcgttatga aaagaaaaca cctacttgct
    27361 tcaagccgga acgattttgc gtagcaaaag aataccgagg aaaagggtta ggcggtcagt
    27421 tgcttgaagc tgctgaaaaa cgggcggttt cagaaggttg tcagactagt tatgtagtag
    27481 ctgaaatgac ggctgtagat ttttatgaaa aacaagggta tcatacttgc acagaacctt
    27541 ttattgaaga tgaagtactt tgtatcggaa tgaaaaaaag tctagcttaa aaacaagaga
    27601 ttgtgacacg ccttttgtca caacctcttg ttttttattt atgtgaattt ttcttatttt
    27661 tacgagcagt acggctttgc tgtctgcgtt cgattttgcg ttctttttta tttttttctg
    27721 caattgccca atcgattttt ttcttataac caggcttgat ctttttcttt ttcttcttca
    27781 caagaccaat cagcgtggga tctaatacat cttttgattt ttctcgtttg gcacgtctat
    27841 tacgatcgta tgttttgacg atttccccgt ttttgatttc ttttggttca aatgagacgc
    27901 ccaatttttc tatcgcatca atggcttgct catcatttgg cgaatataac gtaatggccg
    27961 ttccttttaa gccattacgt ccagtacgtc cgacacgatg gataaagaag tctagatctt
    28021 ctggaatttc tgcattaata acatgagaga caccttcgat atcgatccct ctagcagcta
    28081 aatcagtcgc tacaacatat tgatactcca gattctgtac ttgtcgcatc actcgttttc
    28141 tctctcttgg ggtgatgtct ccatgaatct ttgctacttt caagccttgg tttttcaagt
    28201 aatcggcaat ttcatccaca tgctgttttg tattggcaaa tacgattgct aaataaggat
    28261 gaccaattgt cagcagttga taaatcattt gattctcatc tttacctttt gttgaaatca
    28321 gccaattttc aatgtcttca gaaataactg cttttggttt gatttcttca atgattggat
    28381 tttccatgta ttttcgtaag aatggacgaa gtttttctgg aatagtggca gaaaaaacta
    28441 aaaactgcag cttttctggt aatcgacttg caatttggtc tacttcattt aaaaaaccca
    28501 tatccaatgt catatccgct tcatcgacaa caaatgcaaa tgcggtgtga atcttcagcg
    28561 cttgttcatt catcatgtct aatattctac caggcgtgcc tactacaacc tgtggctgtt
    28621 ggtgatgcag acgatttaat tgacgctgtt tgtctgtacc gccgacaaaa ttcgagacac
    28681 ggatctccgg tgtagaaaat ttagcgattt gtgtagctgc ttcatagatc tgtgcagcta
    28741 gttccctact tggagctgta ataacaactt gtacttcctc tttttctgca ttgatttttt
    28801 caaacaaagg caacaaaaac gtatgtgttt ttcctgtacc agtttgagat tgcccaatga
    28861 tattttttcc ttttgcaatt aaaggaatca aacgttcctg gacctctgtg ggctgtttga
    28921 agccctttgc ggttaacgct tcattgataa acggtttgaa gcggaatata tcaaaattat
    28981 tcattcgtgc acctcttctc tttaaattat cacgactttg gcattataac acagacaaag
    29041 cgtttttgct cagctaattt cgagaatcat catcctttgt ttggccaatc gtgctaacag
    29101 tatatgtgtg actagctttt tcataaaaca gaatccgttt ttttctcaaa aatgatagca
    29161 agatatttaa aataaaaata ccttgtagta acaagacacc gagagaaatg aatactgcac
    29221 tcatctgcaa caccgtattc gttgtcgaat tcatgatttg ctgaagcaac tgccccaacc
    29281 agcttaaacc ataaaaaaga aagtacagat acccgtagcg gatcatcaat gcttttaaag
    29341 aaattcgttc gcggtcttct tccaccaaac ggatacgcac aatttttttg ccaagtgtcc
    29401 atccattggt tataaacggt acaatcataa agtatacccc tacttctaat aaaagccacc
    29461 agatatgaga agtaactgcc cctgaatcga acgcaatttt aaagaaaatc gccgctacca
    29521 aattaataag agataaaaat gtccagtcga tcaaataagc gaccaagcgt cgagtcaatg
    29581 agacagtctt tcctttttca taagaaagtt catccattcg tcttcttgta ggaagcataa
    29641 atgtaaataa cggcgtaacg acaaaaccaa tgattccacc caatgtattt gtcactaaat
    29701 cattcacatc aaataagcgg tatggtcttg ggtagataaa ataaagtcca cttagctgcg
    29761 tcaattcaaa aaatagagaa aggcagaaac tagcaatcat cgttttcata aaagaaagcc
    29821 ggaaataata tcttaaataa atgccaaatg gtatgagtaa taaaacatta aaagctggtt
    29881 ccaaaaatac actctgcttc attgccggca ggtaggtact aggttcagac aagaccaata
    29941 ccgtgttgtc tctgaaatgt tgccatgaag caaataaatg cagctccatc tttggccctg
    30001 tcatttgggc aaccgtctcc tttgccggaa gtggtaagat caccaaaaaa tacgcacaca
    30061 ataaataaaa aacgaaagaa tacaaaataa ctgcccttgt tactagaaat gttccatact
    30121 ttcgatattc ataaataaaa aaaacagatg agatcgctaa ggctaaaaat ggaaaaacaa
    30181 tcagcgcaaa acgaatcggt tcgatataac ttgccatatg actcctcctt gattctttgt
    30241 atctctctta tcttagcagt cctttgctgt ttcaagcaag taacgaggct attatttctc
    30301 atttctttca aagtaaagta agataaacac aaagggggtg tcgagatgct gaatatgtac
    30361 tttgtttttg gcgtacctat ttttttactg ttcctttatg ctacgatcgc ttatgtaaga
    30421 aaaagaacta caattcatta tttaggattt attctcctga ttatttcggg tttcatgcta
    30481 gtcttcaatc ttcagacttg gcagcaagca cttcttgaaa tggataaaat gactccacat
    30541 gctttgtcta aagtgcttgg ctaccctgtc tatctgattt ggcttcccat tttcatttct
    30601 ggttgcttgg ttttattaaa tatttatcgc ggcgtacgcc gaatagttca gttacgcaaa
    30661 tcaaagtaaa agacagaaat cggttgagcc cgatttctgt cttttacttt gctatttcat
    30721 cgtagacaaa acgacattta cgtgatcaaa attaacgagt acttttgaca tttcttccac
    30781 gtactcctca tttaaataaa atattttgtt tggcagagaa gtgcagttct aataaaattc
    30841 gaatgtttta gctatctacg ttttaaatag agataaatcg ggataccagc aagtgtcaat
    30901 actatcccca tcgaagctaa aaaggtctga gtgataagcg tgcttgatac gataaaagtc
    30961 cctccaatga ttgctactag tggaataaac ggatacattg gaactttgta tggtcgaaac
    31021 aaatcaggtt cttttttacg aagaagaatg acaccaacaa agaccatcgt ataaaaaatc
    31081 caaatgacaa agatcagcat atccgttagt gtatcaaaac caccgatcat catcatgcca
    31141 atagcgatga ctagctctaa gatacctgcg gctacaggaa ctttagtttt atcatgaagt
    31201 ttagcaaaga gcttgctaaa aggcaggttg ttttcttttg ccataacata agggagacgc
    31261 attccagtaa gggtatagcc attgatggtt ccataaacag aaatcaagat accaactgtg
    31321 accagtcttc caccgaagcc gccaaaaatc atttttgcaa cgtcacttgc cgcattgctg
    31381 tttcctgcga ctccgtcgat agatgcggtt cttaagaaca ccgcatttac taataaataa
    31441 acgatcatga ttccgatgat tcctaaagaa attgcttttg gcaaatcttt cgccggcttt
    31501 ttcaattctc ccgaaatatt tcctacgtgt atccaaccgt cgtatgcgaa catcgttgct
    31561 agcaagcccg ctcccaaggc tgagaaaaat gaaagatttt ctcctgcttg gatcggaaac
    31621 agttggaagt ctactccttc ttgtcggaac aaaccgaaaa tgacgatcac aaacaaagga
    31681 ataagcttgc agacaagcgt aatcgactga aaagcccctc cagctttaga acctaaaaaa
    31741 ttgatcaaca ggatcgatac agcagctgta acagctacgg gaacaatcat tgactgggaa
    31801 agaccaaata gattgacgaa ctgtgtaccg aaaatgatcg acaaggctgc tacatttgct
    31861 ggaaaataaa tcacgacttg cgcccagccc aataaaaaag ctgctgtatt tccgtaaatc
    31921 cgttcgatat atttaatcat ccctcctgtt tctggaatag cagctgccag ttcagctcct
    31981 gttagtcctg cacagacaga aatcatacca ccgagaaacc aagaaaacat atgcaaactg
    32041 gcggatcctg taacttctgc tacgcttgcg gctttaaaaa atacccctgc accgatcact
    32101 gtgcccataa cagtcgataa agcagtaaaa aatcccatcg tacgttttaa ttcattttct
    32161 atttttactt cttttactgg cgtggcttga tattcttcca tcccttgtcc cctttcatac
    32221 catagccaat cccaaaagta taaaaaactt tttggatgtg gttcttgttt tataccgtct
    32281 ctaccaattt ttctttcagt ttttccaaca tatcctttgt catcacatct aagtcgtatt
    32341 tcggcgaaaa gccccattcc tcttgcgcac aagaaacatc tagtgaattt ggccaagatt
    32401 ctgctattga ctgacgtaca ggatccacat cataatccat ttcaaaatct ggtatgattt
    32461 tttgaataga tgctttgatc tcttccgggt caaaagacat cgccgatata ttgaaggcat
    32521 ttcgatgaac caatcgatca ggatctgcat tcatgagttt tataatggca tcgatcgcat
    32581 ctggcatata catcatatcc attgatgtac cttgatcaat gaaactagtg tactttcctt
    32641 ctttgattgc tgaataataa atatcgactg cgtagtctgt cgtaccgcct cctggcaagg
    32701 ttgcataaga aatcaatcca gggaaacgga caccacgagt atctacgcca tatttcacat
    32761 aataataatc acatagtagt tctccagcta ccttcgttac cccatacatg gttgtaggac
    32821 gctgaagagt atcttgtggg gtatgatctt ttggagtatt tggaccaaac gcgccgattg
    32881 aacttggcgt aaaaaatttc agctggaatt ctctagcgac ttccaatgca ttgaccagcc
    32941 cagtcacgtt caaattccag gctaattgtg gttttgcttc tgcaacagct gacaaaagag
    33001 cagccaaatg aatcaatgtg tccacttgat attgttctgc tatgctgcgc attttttcgt
    33061 aatccattac atctaggatc tcaaagatat cgtttttctc aatgacttca ggcatgcgta
    33121 tatctgtcgc aatgacattt tcactgccta gttcttgacg tagccgtgtg atcaactctg
    33181 aaccaatttg tcctaaacat cctgtcacca atactttttt catgatgatc ctccctgatc
    33241 ttttttacta ctatcttcag atgattaaca atatatgcgt gaacatgttc tctaatttta
    33301 aatagacgaa aaaactaaat ttagttaaat aatttccatt tcttttccta ttttttgata
    33361 aactgctact gcttcatcaa gcatttccct cgtatgttcc gcagtcggca tatttctgat
    33421 cctgcctgtc ccaagaggca cagttggata aacgattggt ttagcataaa tcccatgttc
    33481 gatcagcttc ttagagaaag tctgagtcac tttttcgtct cccaaaatga ctggagtaat
    33541 aggtgtttca gacataccga tgttatagcc tacttttttc aattcttctt taaaataatt
    33601 agcattttcc caaacttttt cgacaagttc cggatgttct tgcatcaaag taatcgaagc
    33661 caatgcagct gcagctgcac ctggagttag cgaagtagaa aataggaatg gccgacttct
    33721 agctttcaac cagtcgatta gtgttttcga tccagctaca taacccccaa caacacctat
    33781 agctttcgat aacgtgccca tttgcatatc gattttgtcg cttaatccaa aatgcttgac
    33841 ggttcctgca ccttttccag ttacgcctga gccgtgcgca tcatcgacgt aggtgatcag
    33901 tccatatttt tcaacaatcg gaataatttc cggcaatcgt gcaatgtctc catccatgga
    33961 gaaaacacca tctgtgatat acatgatttt tttatatttc ttactttcta tcgcttcctt
    34021 tgcttttttc tcaaggtctt tcatatcttg atgtttaatt cggatgattt ttgctccgga
    34081 taagcggcaa ccgtcaataa tagatgcatg gtttaattca tccgataaga tagcatcttc
    34141 ttttgtcatt actgcagaga tagcgcccat attgcagttg aatccagatt gaaaggcgat
    34201 tgctgcttct gtccctttga attctgcgat tttttcttct aattgctgat ggattttcag
    34261 cgaaccattg atcgttcgga ctgctcctgc tcctacacca taagtttcgg ttgcttcaat
    34321 acatgctttt ttcaattctt cgcgattggc aaatccaaga taattgttgg atgctagatt
    34381 gatcatcttt tttccgtcta cgatgataca agcaccattt tcactttcta gtatgtcgat
    34441 cgtattgtat aacccctttt ctttcagttg atccaattct ttctctaaat acattgttag
    34501 ttctttggaa gacattgtct ccacctccat attcatctgt cttgcagaac ataccaaaca
    34561 tatatcgtgt cattcatcta caaagcaagt atgctatatt tttgataacg cttacactga
    34621 ttatgagaat tatttaacca cattttttat ttacagtatc tttttacttt caaatgactt
    34681 atatagtatg aacaattacc ttt
//
LOCUS       DACDTO010000021        32178 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDTO010000021 DACDTO010000000
VERSION     DACDTO010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.021"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(24..203)
                     /locus_tag="IWK70_001061"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="IWK70_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="IWK70_001062"
     CDS             complement(407..766)
                     /locus_tag="IWK70_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9613627.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="IWK70_001063"
     CDS             complement(796..1137)
                     /locus_tag="IWK70_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613628.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="IWK70_001064"
     CDS             complement(1134..1808)
                     /locus_tag="IWK70_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9613629.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IWK70_001065"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IWK70_001065"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9613630.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="IWK70_001066"
     CDS             complement(4322..5512)
                     /locus_tag="IWK70_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9613631.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="IWK70_001067"
     CDS             complement(5533..6060)
                     /locus_tag="IWK70_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9613632.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="IWK70_001068"
     CDS             complement(6107..8896)
                     /locus_tag="IWK70_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9613633.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="IWK70_001069"
     CDS             complement(9043..9243)
                     /locus_tag="IWK70_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9613634.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="IWK70_001070"
     CDS             complement(9696..10016)
                     /locus_tag="IWK70_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613635.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="IWK70_001071"
     CDS             complement(10519..11637)
                     /locus_tag="IWK70_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613636.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="IWK70_001072"
     CDS             12108..13415
                     /locus_tag="IWK70_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9613637.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="IWK70_001073"
     CDS             13536..14735
                     /locus_tag="IWK70_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9613638.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="IWK70_001074"
     CDS             complement(14762..16030)
                     /locus_tag="IWK70_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9613639.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="IWK70_001075"
     CDS             complement(16200..16841)
                     /locus_tag="IWK70_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9613640.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="IWK70_001076"
     CDS             complement(17250..17753)
                     /locus_tag="IWK70_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9613641.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="IWK70_001077"
     CDS             complement(17876..18640)
                     /locus_tag="IWK70_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9613642.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="IWK70_001078"
     CDS             18748..19023
                     /locus_tag="IWK70_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9613643.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="IWK70_001079"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="IWK70_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9613644.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="IWK70_001080"
     CDS             complement(20579..20980)
                     /locus_tag="IWK70_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9613645.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="IWK70_001081"
     CDS             complement(20994..21803)
                     /locus_tag="IWK70_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9613646.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="IWK70_001082"
     CDS             complement(21790..22680)
                     /locus_tag="IWK70_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9613647.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="IWK70_001083"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="IWK70_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="IWK70_001084"
     CDS             complement(23348..24289)
                     /locus_tag="IWK70_001084"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP9613648.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IWK70_001085"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IWK70_001085"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9613649.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="IWK70_001086"
     CDS             complement(25274..26470)
                     /locus_tag="IWK70_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9613650.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="IWK70_001087"
     CDS             complement(26488..27216)
                     /locus_tag="IWK70_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9613651.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IWK70_001088"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IWK70_001088"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9613652.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="IWK70_001089"
     CDS             complement(28628..29086)
                     /locus_tag="IWK70_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9613653.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="IWK70_001090"
     CDS             complement(29083..30546)
                     /locus_tag="IWK70_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9613654.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IWK70_001091"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IWK70_001091"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9613655.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="IWK70_001092"
     CDS             31602..>32178
                     /locus_tag="IWK70_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9613656.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6920 c         5511 g        10362 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DACDTO010000022        32159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDTO010000022 DACDTO010000000
VERSION     DACDTO010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.022"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(97..477)
                     /gene="yajC"
                     /locus_tag="IWK70_001093"
     CDS             complement(97..477)
                     /gene="yajC"
                     /locus_tag="IWK70_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9613657.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(574..1719)
                     /gene="tgt"
                     /locus_tag="IWK70_001094"
     CDS             complement(574..1719)
                     /gene="tgt"
                     /locus_tag="IWK70_001094"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9613658.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1937..2635
                     /locus_tag="IWK70_001095"
     CDS             1937..2635
                     /locus_tag="IWK70_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9613659.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2803..5442)
                     /locus_tag="IWK70_001096"
     CDS             complement(2803..5442)
                     /locus_tag="IWK70_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9613660.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6061..7092)
                     /gene="queA"
                     /locus_tag="IWK70_001097"
     CDS             complement(6061..7092)
                     /gene="queA"
                     /locus_tag="IWK70_001097"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9613661.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7285..8460
                     /locus_tag="IWK70_001098"
     CDS             7285..8460
                     /locus_tag="IWK70_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9613662.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8464..9105
                     /locus_tag="IWK70_001099"
     CDS             8464..9105
                     /locus_tag="IWK70_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613663.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9102..10019
                     /locus_tag="IWK70_001100"
     CDS             9102..10019
                     /locus_tag="IWK70_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9613664.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10016..10687
                     /locus_tag="IWK70_001101"
     CDS             10016..10687
                     /locus_tag="IWK70_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613665.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11228..11716)
                     /locus_tag="IWK70_001102"
     CDS             complement(11228..11716)
                     /locus_tag="IWK70_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9613666.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11917..12183)
                     /locus_tag="IWK70_001103"
     CDS             complement(11917..12183)
                     /locus_tag="IWK70_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9613667.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12183..12446)
                     /locus_tag="IWK70_001104"
     CDS             complement(12183..12446)
                     /locus_tag="IWK70_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9613668.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(12439..12711)
                     /locus_tag="IWK70_001105"
     CDS             complement(12439..12711)
                     /locus_tag="IWK70_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613669.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            12922..13107
                     /locus_tag="IWK70_001106"
     CDS             12922..13107
                     /locus_tag="IWK70_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9613670.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13249..15072)
                     /locus_tag="IWK70_001107"
     CDS             complement(13249..15072)
                     /locus_tag="IWK70_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9613671.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15670..18375)
                     /locus_tag="IWK70_001108"
     CDS             complement(15670..18375)
                     /locus_tag="IWK70_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9613672.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18681..19628)
                     /locus_tag="IWK70_001109"
     CDS             complement(18681..19628)
                     /locus_tag="IWK70_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9613673.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19818..20087
                     /gene="rpsN"
                     /locus_tag="IWK70_001110"
     CDS             19818..20087
                     /gene="rpsN"
                     /locus_tag="IWK70_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9613674.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20263..20625)
                     /locus_tag="IWK70_001111"
     CDS             complement(20263..20625)
                     /locus_tag="IWK70_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9613675.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20636..21757)
                     /locus_tag="IWK70_001112"
     CDS             complement(20636..21757)
                     /locus_tag="IWK70_001112"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9613676.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21773..22123)
                     /locus_tag="IWK70_001113"
     CDS             complement(21773..22123)
                     /locus_tag="IWK70_001113"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9613677.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22253..23764)
                     /locus_tag="IWK70_001114"
     CDS             complement(22253..23764)
                     /locus_tag="IWK70_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9613678.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24086..25438)
                     /locus_tag="IWK70_001115"
     CDS             complement(24086..25438)
                     /locus_tag="IWK70_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9613679.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25591..26667)
                     /locus_tag="IWK70_001116"
     CDS             complement(25591..26667)
                     /locus_tag="IWK70_001116"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9613680.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26863..28608
                     /locus_tag="IWK70_001117"
     CDS             26863..28608
                     /locus_tag="IWK70_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9613681.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28888..29325)
                     /locus_tag="IWK70_001118"
     CDS             complement(28888..29325)
                     /locus_tag="IWK70_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9613682.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29309..30682)
                     /locus_tag="IWK70_001119"
     CDS             complement(29309..30682)
                     /locus_tag="IWK70_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613683.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31004..31492)
                     /locus_tag="IWK70_001120"
     CDS             complement(31004..31492)
                     /locus_tag="IWK70_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9613684.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31514..32116)
                     /locus_tag="IWK70_001121"
     CDS             complement(31514..32116)
                     /locus_tag="IWK70_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9613685.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9191 a         6877 c         5939 g        10152 t
ORIGIN      
        1 gcaatatatc ccatgctctt atctctttct tttttgaata aaaaatgagg taggacatct
       61 ttctgcccta cctcgtcttt aaataagctt tgatacttat ttttcttcct tctcttctgt
      121 ctcttccagc gtttctgttg tttcagtttt tacttcaact gtctcagctg gttcttctgt
      181 tgtttcaaca gtagtcgttg catcatttgt tacgacagtc cctggtttga ccgttttgat
      241 tgctgcacga tcgtactcaa ggaagatccc ttcgcagtct aaaacaactg tccgtttgtc
      301 attgtcgatt tctgaaacaa ctccatgtaa tccgccgatc gttacgactg aatcacctgg
      361 tttcatgcta tctaacaagt tttgacgttc ttgctgttgt ttcttttgcg aacgtgacat
      421 aaaaaaccac attgctaata gcgcgataaa catcacaagc attggtaatg ttcccatttt
      481 tttcacctca ctaattctgt atctacaaaa aacactttag cagaaaaatg ctgataacac
      541 tagtttttta cttaattttt aaaagatttt cctttagaag ctttttgcgt tttctttatt
      601 gaacccgtat tcttcaaaaa aggcttctct aaattcaagc aaattatcat ccatgattgc
      661 ttgacgtact tgtttcatta gattcaacaa gaagtataaa ttgtggtaag aagtcaaacg
      721 aatcccaaat gtttcatcac atttgatcaa atggcggata taggcacgtg tatagttttt
      781 acatgtataa caatcacatt tctcatcaat tggacggaaa tcatgggcat atttcgcatt
      841 tttcactact agacggcctt gagaagtcat acatgtgcca tttcgagcga ttcgagtagg
      901 caagacacag tcaaacatgt ctactccacg gataacacca tcgatcaatg aatcagcagc
      961 accgacaccc atcaagtaac gtggtttgtt ttcggggatc aacggtgtag tgaattctaa
     1021 tacacgattc atttctgatt taggttcccc gacagataac ccgccgattg agtaaccagg
     1081 gaaatccatc ccgactagat ctttcgcgct ttgacgacga agatcttcaa accctgctcc
     1141 ttggatgatt ccaaataatc cttgacggtc tggattagca tgagctttca atcctcgttc
     1201 tgcccatcga gacgtacgtt ctactgattt tttcacgtag tcgtaacttt catcgaatgg
     1261 tggacattcg tcaaaactca tcatgatatc agagcccaat ttattttgga tgttgattgc
     1321 tttttctggt gataaaaaca tacgcgaacc gttcaagtga tttttgaaat gcacgccttc
     1381 ttcttcgata ttccgcatat cacttaatga aaaaacttga aagccgcctg aatctgttaa
     1441 aatcggttga tcccagttca taaatttatg cagtccgcca gcttcttcta ctaagtcttc
     1501 accagggcgt agccataaat gatatgtatt acttaaaata acgccagcac ccatttcttt
     1561 caactcttct ggggacattg tttttactgt agctaatgtt cctactggca tgaacattgg
     1621 tgtggggaaa gttccgtgtg gcgtgatcaa ttcccctaaa cgcgcgccag tatgtttttc
     1681 tttcttgatt aaacgatagc gaatggcagg ttgcgtcatt catttcccta ctttctatat
     1741 aaaatatctt gacaactccc accatcataa tcgtttcaag tgctttttgc aagttccctc
     1801 ggcttccttc tactttattt aatattcttt tttttatcct ttttttatga ataacaaaaa
     1861 aaagaaaaac attatgacag aaatattttt tgatacatgg tatattggtg ttattaaaga
     1921 aaaggaggga aaaatcatgc aatcttctgc agaattaaaa cgccaagcaa aagagagtct
     1981 aaaaggacgt tggggacaag ctgttttact aaatctaatc cctacattga ttggtattgc
     2041 tactattctt cttattatga ttcctattat cttgtttgcc accaccatat atgatggatc
     2101 agcgagcgtc tcagaagcga caacttcagg aggcggtagt tcaggaggcg gtggcggaat
     2161 tatttccagc atcattagcg ctttgttcat gagcggtatt tcttggactt atcttgatat
     2221 tttacgtgga caaaaagatc agatcgaacc attcaaagat gcatttcgtg gattcaaagg
     2281 tttgtttatc ggcggagtga tcagcttagc tgtgctgatc tcgatcttta caacattatg
     2341 gggtttgcta ttagttattc ctggaatcat taaaagctat tcttattctc aaagctattt
     2401 tatttactac gatatcgtcc aagaaacagg acaaacacct aaagtactgg atacgatcac
     2461 tgcaagtcga aaattgatgg atggttataa aggacaactt ttctggttag atctttcatt
     2521 tatcggctgg catatcttag cattagcaac tgctggtatt ggttatctat ggctcagccc
     2581 atacatttct gcaacaaaag cagcatttta tgaacaattg ccaaaagata tttaaaagag
     2641 agtgtgactt gtgaccgagg gggtggcagg agaacaccgt ttatcgagag gcagtaaaag
     2701 aaacattcag cccattagca aaaaggaggt gtgacaacat tcatgtcaca cctccttttt
     2761 gtatagttcc agtaaaaaga aaatcaactt agaaacagtt aatcattcct cttcttctgc
     2821 ttccggtaat cgatggaact tgatcagttc attcaccgcg attggtacga tagataaaat
     2881 ccctaccaag aaccagtgat tcaaactgat gtgtactagg ccgaatagtt cttgcgtaaa
     2941 tggcagcaaa gcaacagccg ttgtaatagc taatgccagt aacgccattt ctaccaaagc
     3001 tttattggtc gctaaattga ctttgaagat cgattggctg ctgcgtacat tgaacacatg
     3061 cagaatcgat gaataagcta agatcaagaa tgcaacagtt tgaccgacat gctgacttgc
     3121 gtctacccat gggctgatag tatcgacata actacctaaa taataaccaa acaaggttac
     3181 gatggtgaag acccctgcat ttatggcaat tttttgcaat aaacctcgag caaagatccc
     3241 ttcatttttc ggaatgggtg gctgatccat gatgccgtgt tccgctggct ctcttcctaa
     3301 agcaaatcct ggaagtccat cagcaactac attgacaaac agtagttgca cagctgtaaa
     3361 tggtgctccc catcctaaca gcatcgcaat caaaacaata aagatctccg aaatattaca
     3421 gcttagcaaa aagttgatag ctttacgaat attctgataa accgaacgtc cttgactgac
     3481 agcgtctacg atcgtcgcaa agttgtcatc cgtcaaaatc atatctgcgg cactttgcgc
     3541 cacatctgtt cctgtgatcc ccatggcaca cccaacgtta cttgctttca acgctggtgc
     3601 atcattcaca ccatccccag tcatcgctac gactgcaccc gtacgttgcc aagatttgac
     3661 gatccggatc ttgtcttctg gcgttacccg agcatagaca gagagagctt tgacacgcgc
     3721 atctaattct tcgtctgata attgatgcag ttctgatcct gaaagtgctt ctgacggatc
     3781 attcaagata cctaattctt tcgctatggc actggcagtc accacatgat ctccagtgat
     3841 catgactgtt ttgatgccag cctttttcgc tcgcttgatt gctcctttac tttctggacg
     3901 tggtggatcg atcattccga tcagaccaat caaacgtaag tctttttcca gtgcttcaga
     3961 agaaatatct tgcggttctt cgtcataagt agcataacca acagcaatca ctctcagtgc
     4021 ccgtttacca aagcgatcat taacgatcgc cgcttgttct acatcgcctg aactaaagcg
     4081 cggagcaaga acatcgaatg ctcctttggt gattgagatg tatttttcgc ccatttggtg
     4141 aactgttgtc atcatttttc gttcagaatc aaaaggaagc tcgtttactc gtggatattt
     4201 ttcttctaat tctgcctttg tatgataatt ctcttcaaca gcccttacga tggctacttc
     4261 tgttggatta cctttgataa caaggtcgtc atcttcttgc tcgaccgtca catctgtaca
     4321 tagtgcagcc attttcaata tttccatcgc gctgttcgtc attgaatctt cgatattcgt
     4381 tacttcatct tctttgctcc atacccggcg gacgcgcatt tcattttgtg tcagcgttcc
     4441 tgttttgtct gagcagataa cattagcggt tcctagcgtt tcaactgctg gcaactgtcg
     4501 gatgatcgca tgtttcctcg ccatcttctg cacaccgtat gcaagtgtca acgtaacaat
     4561 aacagtcaag gtttccggta cagctgcaac agccaaggaa atcgaagtca tgaacatctc
     4621 aaggagtggt tctccttgca gttcaccaat cacaaatact aaagccgcag ccgctaaagc
     4681 gatcatactg attcgtttgc ctagctgatt caatcggacc tgtaaaggtg ttttttgcat
     4741 agtattttcg ttcaaaagat ccgcgatctt acccatttct gtagccatgc caatggcagt
     4801 gacgatcgct ttcccacgac ctgcagcaac cgtacatcct ttaaatacca tattaagttg
     4861 gtcacctaat gactcatctt cttcagcaat aaatgctgca tctttttcaa ctgcttcact
     4921 ttctccagtc aacgccgatt cttctacacg taattgtgat gcttcgatca aacgggcatc
     4981 ggcagaaata gcatcaccgg actctaaaac aagaatatca cccactacca gttgctctga
     5041 ctcaacagtg gtcactttgc cgtctcgtat gacagttgcc gtttgtttat tcatatcttg
     5101 caaggattcc agtgcttttt ctgcgtttcc ttcttggaca atcgccaata ccgagttgat
     5161 cacaacgata gcgatgatca gcagtccttc aaataaatcc ccatgttctg tagcaaatgc
     5221 ggtataaaag gaaatcaccg ctgctacaag aaggatgatc gttgtaaaat cggacaagct
     5281 tcgaaagaat tttttgatca tcgattcttt cggcgcctca tcgaatttat tcaatccctg
     5341 cttttttcgc cgttcatcca cttcttgtga agacaaccca gtatctggat ctgcttctgt
     5401 taccttcttt acttcttcta ttgtctgctt gtattcttcc atcctattcc tcctttaaaa
     5461 tcttcgtaca aaaaaaggta cttattttac tataacatcc tttttttatt tacttcaaat
     5521 aaagggctta tcgattattt gttaagaaag cgtaaaattt aaagtgctta tatttgcaaa
     5581 cgctaccaaa ggatgctatt atatagatgt aaggaaggag gataccatca aatagacaga
     5641 agaatttctc gatagaacag tgtaagcaaa caaagttgtt agaagtacag gtttcaccca
     5701 gacatgcgtg tagagtacaa acctataaat agtgaatgcg tatggatgtt tggatcacga
     5761 agtatccgaa acaagaagta atgcgatacg taacaattga ataacgtaaa cgtctgaaaa
     5821 ttgaatagga acaagagatg tttcaaatgt tgaagatgta acgaaaacaa aatttttgtt
     5881 taacatgaag aaattcaaca ccgtgctttg catgtatgat ggaaagttac aatcatttct
     5941 gtttatttga atccttccga ggtacaagaa agccgagctt atcgagagct cggctttttc
     6001 tttgtcataa aatctttgta aagctttttt gttattcaaa actagcggct tttcataacc
     6061 ttatttcacg aacatcgcat ctccaaaact aaagaaacga tagcgctctt caatcgcatg
     6121 ctggtaagca gataaagtca attctcttcc tgcaaaggca ctaactaaca tgactaaagt
     6181 cgattttggc aaatggaaat tagttgagaa agcttctacc actttgaatt catagccagg
     6241 cgtaatgaaa atatcagtcc atccgctgtc tgctttgatt tctccattaa atttcgttcc
     6301 aatcgtttct aatgttcgaa ttgaagttgt tccgacagcc acgattttcc cgccattttt
     6361 acgtacttgg ttcaatcgct cagccgcttc ttccgtcaaa cggtagaatt cactatgcat
     6421 atggtgttct tcaattttat ctacgctgac aggtcggaat gtacctaacc ctacatgtag
     6481 tgtcagatag actaattcaa caccctttgc ttcgatctca tcaagtaatt ctttcgtaaa
     6541 atgcaatcca gctgttggcg ctgcagcaga accattttct ttcgcataga cagtttgata
     6601 gcgatctggg tcttccagac gttctttgat atatggaggc aatggcattt ctcccaatga
     6661 ttcaagaatc tctagaaaaa ttccttcata ctcaaagtca atgatccgtc cgccgtgttc
     6721 caattcttct ttcactactg cttgcaatct gccatcacca aaagagattc ttgtcccaac
     6781 tttggcacgt ttcgcaggtt tgatcaatgt ctcccacgta tctccttgtg tattcgttag
     6841 aagaaggact tctaaatgcg caccagtatc tggtttttct ccatataaac gagcaggcag
     6901 aactcgtgta ttattcataa ctagcgcatc accaggctgt aactcttcaa tgatttcatg
     6961 gaaatgttta tcttccatct ctccagtttt ccgatccaaa acaagtaaac gagaatgatc
     7021 gcggtctttt aatggtgttt gtgcgattaa ttcttccggc aagtcaaaat caaaatcttc
     7081 tgttgttaac atatcgtcac tcactttcta tcatgtgctt catcattcta tcatgttttt
     7141 cgttctgttg gcaaaatcag cactttttca cttctatttt ttgttttcct aaataaaaaa
     7201 actaccctat ttgtattttt tcattgtttt aggcatacta gtaatagata gttcttcatt
     7261 gaaaatagaa aaagaggtgg caccatgatt gaatttcaaa atgtgtcaaa aatttataaa
     7321 ggtggaaaag tcgctgtaga ggacgtcaat ctttcatttg aaaagggtga gttcatttgt
     7381 tttatcggta cgagcggaag cggaaaaacg acgacgatgc ggatgatcaa ccgaatgact
     7441 gatccaacac aagggaaaat tttgattgat ggaaaatcca tccaagaaat tgatccagtg
     7501 gaattgcgac gtcagatcgg ctatgttatc caaagtattg gattgatgcc tcacatgacg
     7561 atccgtgaaa atatcactct tgtccaaaaa ttgttgaaag tcagtcaaga agaacggaat
     7621 aaaacagctg aaaaaatgat tgatcttgtg gaattgcccc gtgagatgtt agatcgctac
     7681 ccacatgaat tatctggtgg tcagcagcag cgtattggag tcgttcgtgc ccttgcagcg
     7741 aatcaagata tcatcttgat ggacgagcca ttcggtgcgc ttgatccaat cactcgtgat
     7801 tccttgcaag accttgtcaa agatttacaa gagcgtttgg gaaaaacgat cgtatttgta
     7861 acccatgaca tggatgaagc gattaaacta gcaagccgta tcgccatcat gagtgaaggc
     7921 cgtgtgatcc aatttgatac accagaaaat attttacgtc atcctgcaaa cgatttcgta
     7981 gaagagttga ttggagaaga tcgcctacta caagccaagc caaatgcgac aagagtcggc
     8041 gaagttatgc taaatacagc aatcacgatt acaccggaaa aatcattaca agaagcaatc
     8101 aaactgatga gggaaaaacg tgtagatacg ttgttagtta ccgataattc gaatgtatta
     8161 aaaggattta tcgatgtaga aacgttgaat aaaaaacgag gaaaagtatc cagtgtcggc
     8221 gacattttga ataaacatgt tttttacgta aaagaatccg cctatcttcg tgataccttg
     8281 caacgcattt taaaacgcgg gctgaaatac gtgccggtcg ttgatgaaca gaacaaagtc
     8341 gtaggtatcc tgactcgtgc atcattagtg gatatcgtct acgatgtgat ttggggagaa
     8401 gatcccgcaa ctactgatgt tgcagagtca gctactcctg aagaatctaa ggaggtctga
     8461 cctatgcagc agttttttca tcagcacggt tcagaaattc tctcaaaagg ttttgaacat
     8521 ctttatattt caggacttgc tttgatcatt ggtatcatca ttgctgtacc gcttggtgtc
     8581 ttactgacac gttttccaaa agctgccacg attgtgattg gcttaaccag tgccttgcag
     8641 acaatccctt ctcttgcttt attggcattg atgatcccgc tatttggcgt aggtaaatta
     8701 cctgcgatta tcgcactctt tatttattca ttattgccga ttttacgtaa tacgtacatt
     8761 gggatgaaaa atgtcgattg gaattatcgg gatgtggcaa aaggcatggg gatgactgag
     8821 ttccaatcca ttttttccgt tgaattgccc attgctatgc caacgatcat ggctggtatt
     8881 cgtttagctg ccgtttacgt gattgcatgg gctacacttg cctcttatat cggcgctggt
     8941 ggtttaggcg atttgatttt tagtggactg aataattacc aaccagatct cattttggca
     9001 ggtacgatcc ctgtaacaat tttagcacta ttggctgatt ggcttctagg tctcttggaa
     9061 caccggctga caccgatcgc acttagagag gagaatgaag aatgaaacgt atactaaaaa
     9121 tgaccatggt tcttttctct cttcttatac ttgcgagttg ttccttccct ggtcttgcca
     9181 gcaatacaga tgaagacacc atttcgatca caggtggaat cacttcagaa gcacagatct
     9241 tagcaagtct agttgccgga atggttgaac attatacaga taaaaatacc gcaatcatca
     9301 acaatctggc tactacaact atcaaccatc aagcgatgat gaacggagat gcttcaattt
     9361 cagcggctcg ctacacagga acagatttaa cgactacatt gaaccttcct cctgaaaaag
     9421 atccgaaaaa agcgtttgct accgtgaaag atgaatttga gaaaagatac ggtcaaacgt
     9481 ggttcccgtc ctatagtttt gaaaatacgt atgtctttct tgttcgaaaa gacaccgcac
     9541 aaaaatacca tctatctaaa gtcagcgatc tgaaaaatgt agcggatgaa ttagtagctg
     9601 gtgtagatac ttcttggatc aatcgtaaag gagacggtta tgacggcttc caagaaactt
     9661 atggcttctc cttcaattcg atattaccta tgcagattgg acttgtttat gacgcagtcg
     9721 aagcaggaaa aatggatatt gttcttggct actctacgga tggacggatc gccagttatg
     9781 atctagtcat gttggaagat gatcgtcaat tcttcccacc atatgatgct gctccagttg
     9841 tagacaacaa gatattagct gatacgcctc atttaaaaga agccttaaat aagttaggtc
     9901 ataccatcag tacggaaaaa atgcaggaat tgaactacga agcagataat aatctagtgg
     9961 aaccatcagt agtagcacaa cgtttcttac aagaaaacca ctattttgaa gggaagtgaa
    10021 gcaattggat aaaatgaatg tctttcaaca atttttctat tatttccagg aaaatggctc
    10081 ctatatcttt gcacaattta tccgtcactt cctgatttct atctatggtg tcctctttgc
    10141 agcagttgtc gggattccgg ttgggatcat gatttctagg agacgtaaat tagcaaactg
    10201 ggtgattcgt ttggcgaata tcatccagac gatcccttct ctagccatga tctccatttt
    10261 aatcattgga ttaggcctgg gagtcaatgt cgtgattgtt actgtatttt tgtattccct
    10321 cttaccgatc atcaagaata cgtataccgg gatgatccaa gtagacaaaa acatccttga
    10381 tgtcggcaaa ggcatgggga tgactgctag acaacgacta ttcatggtag agttaccact
    10441 ctctgtttct gttatcatgg caggcataag aaacgcgtta gttgttgcca tcgggataac
    10501 cgcgatcggt gcattcgtcg gtgctggtgg tcttggagat atcattatcc gaggaaccaa
    10561 tgcaacagag ggcacatcga ttattttagc aggtgcattg ccaactgcat taatggcgat
    10621 catcacggac tggcttttag gtattttgga aagacggcta gatccagcca gccgtacatc
    10681 acgctgatgt tattgtaaaa aacaaaagct tctgcgacca tttgttggtc gcaaaggctt
    10741 ttttagttct gtcattttta aggcaataat ccactctgat tcttccctcc ttatgggtaa
    10801 agagttgctt ttcttagcta tttatccatt ccgtatactc caacttcata ctgccaacgg
    10861 gttcatatct gcatccaccg actaagtttt tttcttatcc gctaaggcat tttgccaatc
    10921 cattttccca tcactcgtca tggaattcat accacatgat ctctaaatac tcgtctgctt
    10981 gttctttgaa ttgggcatca attcttacat cctttggcgt ggctttcatt taagcctttt
    11041 ccatcagtcc tcgattgttc gatgccttca gctgcatcat caaaactggt gactgccgta
    11101 tcggtaactt cgatcatctt ttatacaatc gggaacttgc catcctcatc cttggctaaa
    11161 aacatatcta gccattccag cttctcgcca tgggaggttt gcggttccta aaaccggtac
    11221 acgattctta tggactgcat ccgtcgcaac tagacttggc gatacgatga tccttcacca
    11281 gaagagcacc cccgtaaacc aattgatcga tatattgcca ataagaaaag acattggcgt
    11341 aaaagatatc gatccctcgt gatgcattgc cgttgatact gctattcatg gccgaaagcg
    11401 ccacgacttt aacttcttcc gtttgagtga cattcatgtt cggcaatgtc gctttggata
    11461 cccgctctgc taaaggaacg acactggtat tgtatttcgt atctgtatct tttccaaaag
    11521 accatgccaa cgaatcctct gggaaccaat aagacgactc cggttgattg tccattgctt
    11581 ttttcaacat acttttggct gtttctcgat aaacgacatt tccaattgtc ttcgcctcac
    11641 tttggcgata atcgatggtc gttgccactg ccaacactaa caacgatgct ccaattttac
    11701 tgattttcct tgtcacactt cttcctcctt gttttacata aacccgtcaa cattccattc
    11761 ctaaactcaa acaatgaccc ttcaaaggtg gagatatagg aacaaattaa agaaatcatg
    11821 caataaatcc aagaaggttg gttttagaac aaaaaaagag tctccttttt ggggagactc
    11881 tttgttaact atctaaaaag gttggctctt ctcacttcac tcataatgtg tccaggcaga
    11941 ataaatttct acaactttat tttcctcatc aactttgtaa accactcgat gctgtctatt
    12001 cagcctgcgt gagtatctgc cttcatgtgt aggtctcagc ttttcaaaag actgcgtggg
    12061 catatatgga tcttccttca atgtctgaat cacttcttca aattgtttct ttaaattcgt
    12121 ttgcttgatt tttcttaaat ccactttcgc agagtttttg attgcgactg tatagttact
    12181 catcaaagag cttcccaatc aatatcatcg atattcgtaa agctactgtc gtctttttcg
    12241 cggtctcgta ctttatctaa agtacctgtt tgctcaagaa aaagggtttc ttgaatcgct
    12301 ctccagtctt ccagcccaat caatacagca ttgtttccac tacgatcact aataatctcg
    12361 atttcagtat gattttcatt tacctctttc aataactgat aaaaattttt acgagcagtg
    12421 ctaggattca tcgttgtatt agccattgtc tctcctcctt tttctgatat aattatagcg
    12481 tacttgatta agtacgtcaa aaaaatacaa taaagcactt aaattcagca aacttttcct
    12541 cttttttaca tagagaaaaa gacccctttt tagggaggat ctttgtccac gctcacttct
    12601 tactccttta cgactagcgg tagccaaacg taaagtaatt ggtaacaacg tatcatcttt
    12661 agcaaaggtc gaatccaaag cctgtgagtc tatgcaacaa caccaataca cgaaggttcc
    12721 tcgttgcagt gtgacttgat tgatatcttg aataacctga ggatacgcac gaaagatttg
    12781 taacggttat tcaaaactga ttttggccgt caagtcttct actgcctcaa aaatgattgc
    12841 ctctttcgtc tcctgtcatt catgttcact atcgatttga tacttgctta gaccgttggg
    12901 tttgtgaatc accaattaaa aatggcactg agttatggta acaaaaaatg gtcacttgac
    12961 gaggctgaga ttcctactta tgaagacaca tggcacttaa gagtcgaggt aaaggaagaa
    13021 gctgaaaata taggaggctg tacattattt ggcaaaggat tcggaaatta tgatcaagac
    13081 atcaaaatca aaatgtttta cagctaaata ggaagaataa taacatttca tacgctgttc
    13141 ccatctttaa tggcttatag agggcacctt tagctgagaa taagacaaaa cagaccctct
    13201 agcttcaatt aagagctaga gggtctgttt ttgattttaa aacttggttt atttcttcaa
    13261 atggtagcta taggtgctga caacgatatt ttcacgccaa gacaatcgca atcttaaccg
    13321 caaacttgtt tggttatgga gaatattctg ccagcgtctg tttggtacaa attgaggaat
    13381 caacacagta gttgtatagt tttgtttcgc tgcattttta cttacgatat ctacataacg
    13441 gataattgga ttttcaatcg agcgataaga agagtggacg attgaaaaac ggacatctgg
    13501 gaaatgcttt ttaaattctg cttcgatttc tttttctttt tcgacatctt cgtctagcga
    13561 aacatgcatc gcaacgacat aatcaccgat cgaacgtgcg taattcaacg caccgatatt
    13621 tacccgtgtc acattcccga ctaatacaag aacggtattc ccgtcataat catgtagttt
    13681 tgcatcattt tctaaacgaa gctgttctgc tactttttta taatgatcat ggatcttata
    13741 aaaaatgaac ataacgattg gcataatgat gaagaaaggc caaatgtccc ctaaccgata
    13801 gacaaacaaa atcgcaatga ttgcatatga gatgaacgca cccgtaatat tggcaatcga
    13861 tttgcttaac catctttttc cttctttttt ccatttgacg accatccctg tttgagataa
    13921 agcaaatgga atgaacacgc cgatagagta taacggaatc aagcgttctg ttgagccatg
    13981 gaaaataaac agcaatacga tcgaacctag cgctagtgtg ataatcccat tggaatagcc
    14041 tagtcgatcc ccacgatctt gatacatgtg aggcatgaat ttatcttttg ccagattgta
    14101 tgcaagaact ggaaaagcag aaaaaccggt gttagcagca actgctaaaa tcagcgctgt
    14161 agcaaattga aggacgtaat acaaaatacc atgtccaaag acagctttac caatttgaga
    14221 caaaacagtg acttcttttt caggaacgat cccataccaa tagttgataa atgtgatccc
    14281 cacgaagaag aaacctaaga tcaatgccat catcgtcaat gtagcagcgg cattttttgc
    14341 tcttggcttc ttaaagaatg gtacagcatt actaatcgcc tccaccccag ttaaagagga
    14401 agaaccagaa gaaaatgctc gtaaaatcaa agcgatagaa acacccggca cagccgctcc
    14461 tggaagtgca gtagcttgca atggcacagc acctgtcacg atattatata aaccaacaac
    14521 gatcagtaac gagatcacaa ggataaatgt gtacaccgga aataatagga acgatgcaga
    14581 ttccctcagc ccacgaagat tcagcatcat cagtagcaaa acgattgtca cagaaatagc
    14641 gacttgatga ccgtagagtg ctggcaccgc tgatgtgatc gcttcagcac ctgctgagac
    14701 agatactgct actgtcaaca tgtagtcgat caataaagag ccaccggaaa tcaagccagc
    14761 attctttcca agattctcac tgctgacgac atatgctccc ccgccatgag gataagcatg
    14821 gatgatttga cgatatgata gcgttaatga aatcaataaa ataataacaa aagcggcaat
    14881 gggtagcgaa taccagatgg ctgcagcaga caaagcgact aaaacgacaa cgatttgttc
    14941 cgtaccatat gcaatagatg ataacgcatc agacgacaat aatgccaaag cagcaaatcg
    15001 ggttaatttg tgctcatcat tttcagcaga ttttaaaggc ttacctacta acaacctttt
    15061 taagtaatcc aatttctaaa ctcctttaca cacaatttta cttcaagttt tatagtcgaa
    15121 aatttatagt aaacaagatg ttatagtacg ctcactgacc ttaaaagtca ataaacttta
    15181 agaaaataat tggaaaaaaa cagaaaaata tcaagcgctt tttcaaacgc tttaatacag
    15241 caaaaatcta acattttttt atgggctttt tctttgaatt ctataataaa aggatagaac
    15301 ttgacttaga aaatcaagtt ctatcctttt tgatatctta acttttggaa gagagatgta
    15361 aggctgcttt ctacccatca ttcttactac tcgaagcata gcgcagttgt cacaacctct
    15421 tgataaacgg tgtttaaaag cttgcatctg cggtaataag cccaaccaaa ttaaaaacat
    15481 aagaagctgt ttttatttgg tcgttcttat tacttgatgc agagcagctt ttttacaacc
    15541 tcttggtaca cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa acggcaaaat
    15601 atgaggaact atttttccat ttttttctct aatactcagg tcaaaacact gatttcacaa
    15661 cctctttttt taatgaaaaa tatggctctc atgcgccgct tctttgaatt tcgtcatttt
    15721 ttcttcgatg aacggataga ttgccgttcc tacgattcca aagacaacaa acaaaccgat
    15781 catgatccag attgctttcg tagcatttgg ccaataaatt cctcctgcag attcccgtaa
    15841 caagtttacc gcatgagtaa atggcaagaa cggattgatc atttggaaga atttccctga
    15901 gacttggatc ggatagttcc cgcctccgcc agagatcgac agtactaaga tgataatagc
    15961 gatcccttta ccgacattat cgaataatgc agccaagaca tagacgatca tcatgaacgc
    16021 taaggcgatc aagacagcaa atgacacgct gtagaccgga ttttggacat ctactcctag
    16081 taagaaaata ttccctaatg taacgatcaa gctttgcgca atcgccattg tcaaaaatgt
    16141 cagcatacga gcaacgaatt gttctcgttt cgtaaaattg tttcggtctt tctttcctaa
    16201 atagtattct gtcgtagcaa cacttgaaag aagaacagcg ccgacccaca gacatagtgc
    16261 agtataaaac ggggtgctag ctgaaccgtt atttgcgatt ggataaagcg cattggtttg
    16321 tagctcaaca ggttgtgtaa agaagtcact ttcttttgtt gcgtctagtt tcaataattt
    16381 gatgacttcg cctagatcga cttcttgttc gccttttcgg atcgcgtctg ctgctttttg
    16441 gataccagct ttgatatttg gccagtcatt gttgatcaag tccgcagcag cgttcacagc
    16501 tgtttcgact tctggcattt tttcatttac tgttgtcaat gtaccggtga tttcattttt
    16561 tagtgctggc caatcatttt ggataaaatc agcggccaat cctaattttt gctgaatgat
    16621 cggtaattca ttgttgtata ggtctgcccc ttcattgatg ccattgacga tggtatccat
    16681 atttccattc agcattgtat tggcatcatg gatttcttgt ccaattgcag gcatttcttt
    16741 ttgatatttt tctaaaattg ccactgcatt cgttacagtt gctttcgttg agctgagaag
    16801 cgaatcaaag tcgatctggt ccgctttgct cagtgcgcct tgtgcagtcc caatcgtatc
    16861 gatgacttgg tctagtagag aagtgacagt ggaactgatt tgacctgtat tgataccagc
    16921 aatgatgcca ttgatattat taacaacggc atcaatttga tctagcaatt catcaatagc
    16981 cttcgtgtct tcattttgaa cagctgtgtt caaagcagat aggcggtttt gcagattgct
    17041 tagcaagggt tttaattgat ttagctgatc aataattggc tgtaatttcg tatttccagc
    17101 acttgtttgc aatttagtca ggaactcgac cagtttatcg attgcatttt gttgttgaga
    17161 tattccgtca ataaagccgt tcaatacatt gctgatgttt ttcttttcat catcagtcag
    17221 cttaccatct tcaatagctg ttcttacagt atctattaac gaacctgtcg ttgttgccac
    17281 ttgtccaatt gacttcaatg taacatctac actgtttgtg atgcttggta tcgcatcttt
    17341 cagctgagag gcacctgatt ttgtttggtt tgctagatca cttgcctgat tttctaaatt
    17401 tttcacatct ggcaagatat cttgtacttg ttggatgatc gtcaagcctt gttttgcttc
    17461 atcgattcct tggttcattg tctcttggat cgaagcaaaa tcgccgtcga tctctgctag
    17521 ttgcttaccg gcattttgga tctctgggat tttttcttgc aaatcaagga tgatttttgc
    17581 ttgatctttc aactcaggca ttttgtcatt caatgcaacg actttttcac ccatctcatc
    17641 tactttaggg atgtaatcca cgaattcatt tgctttattc agtttttcct tgatttcagg
    17701 tagttgactt tgtaattcga ggacttgttt tgtgtagccg tcgatcgtgt ccaaattttc
    17761 atctgttgat aaaatcatat ctttaacttt attgatactt actaggttcg tatcgatgtc
    17821 gtaacctatc tcattgaata ctttcaataa agtcgaactg gctgttttga taaattcatt
    17881 tgtgatttgc gattggatcg aacttgctcc tttatcggtg attttcggtg caatcgcatt
    17941 gattttttca tttaccgtat attcgatttt tggtttcttg atatcaccac tagtaaaact
    18001 cagcagatct tctgagaaat cctttggcaa gtaaatacct gcgtaatact ttccggaacg
    18061 gactccgtct tctaattgtt cttttgaatc aacgaattgc catcctaatt gtttattttt
    18121 atgaagcgat tcgatgactt gctccccaat ggccacttct ttaccttgaa actctgctgg
    18181 cttgtctgca ctgtaaacag caattggcag ctctccagta tttccatatg gatcccataa
    18241 tgctttgatg ttaaaccacg catatagcga agggatgatc atgatcgcga tgatcagaag
    18301 tgttgcaatc gggtttttga aaatccgttt ccagtcaagc aaatataatc gaagcgtatt
    18361 tttgattgac tgcatagttc tcccccttct tcttttttca gctgaattac tttaccatgt
    18421 atttcatact tttttcaatc ggcttttttc aaaaaagtcg aatttttgga catatcacgg
    18481 caattgtctc attgtaatct cttacatatt gagaaataat taaggattac agcctttact
    18541 ttatcatgaa ataaaaggga aagataattt tatgccaaag tttttatcta ttatacgtat
    18601 acgcaaaaaa gctaaaacat tttttgtctt tgcccctaca aaaatgtttc agcttttttc
    18661 gttttttatt cctgtatttc ttatttttcg aatgacagga tgattttccc agcaggagga
    18721 tgtccttcaa ctagctgatg tgcttggatg acatgcttaa gtgaagcaga cagattcatt
    18781 gaaataaaaa catgaaaggc tcctttttgg taggctttga ttatgccagc aaaggcttct
    18841 gcatctagat attcctttct tggaacaaac gattcataaa agccttcctt tttttgccgc
    18901 aaatctagat ctgggaggtc atttaatgca acatacctgc cgcctggttt catgatctgg
    18961 atgccggttt ctcctttgat acttcctttc gtcgcatcga ttacaaggtc tgcctgatca
    19021 gcaaattgca gacctggatt tgttttatca taagcagcaa atgctgaagc tcctaatttt
    19081 tttacctttt cctcattttt actggatgca ctggttaaga ttcggattcc cttttcatgg
    19141 agaagctgga tcagacttga cccgaccgca cctgatgctc ctagtatcat tacagtatct
    19201 gtcggctgga tttccgtcaa atggttgatt agattataag ccgtaattcc tggcgtgacc
    19261 attccggctg cttcttccca actcatttta tctggaattt ttgctacttt tgctgatggc
    19321 aacacgactt cttctccgta agtcccgccg actgcatgga cagctactcg atcacctaca
    19381 tgaacagttg tgacatctga accaatctct gtcacaatac cagcgccatc attacctggt
    19441 acataaggga atttcaatgt tcgtatctct ttcatttctc ctaaacgcaa ggcaacatca
    19501 taaggattca cactgaatgc ttttatttct acacgaacat gtccagaacc gacttctctg
    19561 ggatgagcat cgatttcttc aaaaacatct tgtgcttctc cgtaaccgtt aattgcaaat
    19621 cttcgcatgg tcaccatctc cctttttctt taagtttacg tttatgcctt tattttgaca
    19681 aaaaaaatgt cttttcgttt tggcgcatga ttcaaaacaa aatagttgtc ttttttcgcg
    19741 taacgtgtta aattatttat ctaaaagcgt aaagattacg atttacgcta ataaaaaata
    19801 attggaggaa tttgttgatg gctaaaaaat caaagattgc caaagcaaaa aaacagcaag
    19861 aaacgattga acgctatgct gctttacgcc aggaattaaa agcgcaaaaa gattacgaag
    19921 gcttgcgtaa acttccttta gatgctcgtc cagaacgatt gaaaaatcgt gatttaatcg
    19981 atggacgccc tcgcggatac atgcgtaaat tcgggatgtc ccgtataaaa ttccgtgaat
    20041 tagcacataa aggtttgatt ccaggagtaa aaaaagccag ctggtaatcc aagtctggct
    20101 gtaatgtaaa ggaggagggc gcatggacca ctccttggat catcaaaaaa gatacgttcg
    20161 cttcttctag cagaaatgct ggtactagaa gcagacgtat ctttttttac gcacagctgg
    20221 tcctttttta tatgtaaagg ctgcatggtt acttcgctat atttatttag taacaggagt
    20281 cagtgcttcg atacccacac taattttcaa tgctcggtca acttctgcca taatgggtcg
    20341 atccaaatgg cagacctttt ctttcaatct tgatttatcg atcgttctga tctgctccaa
    20401 caggataacg gagtctcttt ctattcctgt tccagtagct ttgatcccaa tgtgagtcgg
    20461 aagcttaggt ttagccattt ttgccgttat ggccgcaaca atgatcgtcg ggctaaaatg
    20521 gttacctaga ttattttgaa taaccaaaac tgggcgtgtt ccgccttgct ccgatcccac
    20581 gactggagaa agatcagcaa aataaatatc tcctcttttt accataggat cctccttatt
    20641 cttgatattc tcttggtatc cgctgtccta atccgcaagc cacttcatag tggatcgtac
    20701 ctaattgatc tgctatatcc tgcatagtga tttctttgtc tgcatttttg ccgataagcg
    20761 tcacttttgt tccgacagga aattcttgag gcaatcggat catcagttga tccatacata
    20821 cacgcccaat cgtttcacag tagttccctt ctaccaatag agaaaaaccc tgcatctttc
    20881 gtggccagcc atctgcataa ccgatcggta tcgttccgat ccactctgct tcaggagtga
    20941 tatatgtttc tccatagccg attccttcac ctgctgggag ttttttgact tgaatcaatt
    21001 cagaaaccag ctcaagtgct ggctgcaatg gataaacttc cggtagtgcg tgcccagagg
    21061 gattaagtcc gtacattgct actccataac gaatcatatt gccgattgtt tcatcatgcc
    21121 ataaagccgt tgcactattg ctgacatgta catatcgagg aagttcggga agttttttca
    21181 gcacttctat aaagcgttct ttttgcagat tccaatatgt atcatctgcc tgatctgctg
    21241 tagaaaaatg ggtaaataaa ccttcccatt ctaatacacg actttctttg atgaatgcag
    21301 ctgcctcttt tacttcttca ggtgtacaaa atccgatacg acccatccca gtgtctgctt
    21361 ttagatgaat cgaaagaggc gtctctagct tacttttctc taaaacagca gctgctgctt
    21421 gcaaccattc ccgtgtacct gcagttactg ataaatcatg agcaacagct acagtcagtg
    21481 cgtcgtaagg aaccatattc aagataagga tgggctgagt aaatccagct tctctcaact
    21541 cgactccttc atctaaattt gaaacacaaa agcccgaagc tccacctgca actgctgcct
    21601 cagctgtttc aatagctcca tgaccgtatc cgttcgcttt tactactgca aacaattctt
    21661 ttccttgcgg aagtcttgca acttcatttg ctacattttc agttatagct tttttatgta
    21721 tcactgcttt cgttgggcgg tgccaagcga caaccatcgt ttttccttct ttctagtctt
    21781 ccaaaataat ttgtgcaaaa gctgtttcat ttgtatgagt aatactgacc catacattgc
    21841 cttcatgagg tgaacgcgtc acaacaggct gaccattttc attttttaaa atctcgacat
    21901 cttgaaaggt aacttttccg atcccagttc cccaagcttt agagaaagcc tctttgcaag
    21961 cgtaacgccc gcctaaaaat tctacttggc gatgaaaagg caaggactgg aatagtttca
    22021 tttcatcaga ggtcaagatt cgtgcaatga attttggttt ttcttcgatc agccgagtga
    22081 tcctaggaag ttcgactgca tcgatcccaa ttccttttat catatgcttt tctcttttca
    22141 ataaatttag aacactctca ttctaccaaa ttataaccat tttgccacaa ataagtccac
    22201 aaataacaaa aatgaaagtt tctcttaaaa aagagaaact ttcattttat gtttaatcgt
    22261 tatttgaacg gattacaaat ccgcgttttt tatcattgtg acgtttaccg ccatcttttt
    22321 ggctgttatt atttttgcta taaccgcctt ttgatttgtt tccacgataa ttaccgccgt
    22381 cacgacgatt acggttgttt ccgccaccac gtttgccatt tttattgtat ccttttttct
    22441 gcattggcaa tgggcgttct ggtgtgatct tcactggcac tgcatcagct ggatcttttg
    22501 ctgtcgtttt tagcaaaagt gcaactaaat cttgtgcaga atattcttct aataatttat
    22561 ctgctgtttg caggtattta tctagaccat tttctgcaag ttttgtttcg atttgttcaa
    22621 ctgcagcacc taattgacct ttgaatgctt ctttttcggt tggtggacgt aaagtcgtca
    22681 tccgtttttt cgtcaagttt tcaatcacat gcaagtagtc catttcattt ggtgtaacga
    22741 aagtcacaga cataccgcct tttccggcac ggcctgtacg tccgatacgg tgaacgtagc
    22801 tttctggatc ttgagggata tcatagttgt acacatgagt gacacctgaa atatctaatc
    22861 cacgtgcagc aacgtctgtc gctacaagga tatctaaatg accgctctta aatgaacgca
    22921 atacgctcat tcgtttttgt tgagacaaat cgccatggat tccttctgca cggtaaccgc
    22981 gtgcttctaa tccgcgtgcc aattcgtcta cacggcgttt tgtacgtccg aagacgattg
    23041 ttaattcagg tgtttgaaca tcaaacaagc gagtcatgat atcgaatttt tcgtattctt
    23101 ttgcgcggac atagtattga tcgatcaagt cagctgtcat ttctttcgct ttgatcttca
    23161 catgatgtgg atttttcatg aatttaacgc caatattctt gatagctggc ggcatcgttg
    23221 ctgagaaaag taatgtttgt ctttgatcag gaacttgtga aatgattttt tcgatatctt
    23281 ccaagaagcc catattcaac atttcatctg cttcatccaa aactaatgtt tgaacggttc
    23341 caagtttcaa tgtgtggcgg ttaatgtggt caagcatacg tcctggtgta cccacaacga
    23401 tatgtggacg atctttcaag ccacggatct gacggccaat atctgctccg ccgtaaacgg
    23461 cttgtacacg tacttttttg tcgcgcccta aacgatataa ttcttcttgt gtttggatag
    23521 ctaattcacg tgttggtgcg ataactaaac cttgtaattc gtgacgatct ggatcgattt
    23581 tttctaacat tggtaatcca aatgcagctg ttttccctgt acctgtctgt gcttgtccaa
    23641 taacgtcttt tccagctaat gctagtggaa tcgtttctgc ttggataggc gttgcttctt
    23701 caaaaccagc gcgttcgact gatttcaaca attctggtga taattctagt tctttaaatt
    23761 tcaaatgaat cctcctatat ggttgttaac tgaggatgta gcttttttgc taccccgtcg
    23821 atttgttcgg tcaaagtgta ttgtttcttt ttgacagtgg ccacgcacgt ttttatccat
    23881 tcttagcccc tgtggcacca ccgaaaaatc actttgcgaa gtttaggcat tagaaaagcc
    23941 aaacgggtcg cgttcagctt tatcattatt atgacatctg gcttcaacaa gttgtttcta
    24001 tggcaaaacc agtccaattt ctcttcatct ttcggcaata ggttattcta acatagtttt
    24061 tcgttttctg caagtttaca gatttttatt tcatttgctg aagttgttct accacttcaa
    24121 ataagcccat gctgttgctg ccttttagca taatactatc tgttgcttca agattttgtt
    24181 tgatagcatc aatcaattta ttcttttctt tttcaaaata gaacacaggt agttgggggt
    24241 atttttggtt caattcttct tttagtgcat gcatttgttc cccataaagg aaaatcatgg
    24301 cgaatttctc aggatcgata tgttcactca tttgcgcatg catatttaaa gactctttcc
    24361 ctaattccag catatctgct aacacagcga ttctgcgtcc ttttgttggc agatttttaa
    24421 aagtatctaa taccagtccc atggctgttg gatttgcatt gtagacatcg ctcaaaatag
    24481 ctgcaccatt tcctgccgtt agccattcgg ttctgttttg agtgatagaa acatgggcga
    24541 gcccttggcg tatctcaggt atggacagtc caaagaagcg cccgatcgta tacgcaatca
    24601 aagcattcgt tacattataa cttccaggca gcgggatcgt aaatgtttct ccttctacta
    24661 caaaatcggt ttgtgctttt ccttttgctg tcacttccgc atgaacatcg ccatttccaa
    24721 taccgaatgt tgtgactgtt tgtactaatg ttgccgttaa aggtgtcagc aacggttcat
    24781 ctgcgggcac aacgagtaat ccgtctgata caagtccgtc agtgatttcc attttggctt
    24841 tagcgattcc ttctcgcgac ccaagatttt ctatgtgtgc ttcaccgatc attgtgattg
    24901 ctgcagcttt tggttgagcc ataagtgaaa gttccgtgat ttcattggcg tgatccatgc
    24961 ccatttccaa aacgatcttt tctgtatctt ggggcatgtg caaaatcgtg taaggaagac
    25021 caatattatt gttgtaattt ccttgcgtct tatatgtttt gtatttttca gacaagacag
    25081 acgcggttag atctttcgtg gtggttttac cattgcttcc tgtaattgca acgacttctg
    25141 cgcctacttt ttccaagtaa taggttgcca attgctgcat ggctttcaaa ggatctgcta
    25201 caggaataac tggaaaattt tttggagctt gttcaacagg caagctccaa aatgaagcag
    25261 atgctccgtt tgtgatcgct tgctggataa attcatgtcc atctcgcgta ccagcaagag
    25321 gaacgaataa acttccttcg gttatcttcc ggctgtcaaa ttcgacattg gtaatctttg
    25381 tcagtgcaga gcattcgctg cccacggctt gtgcaatttc ttgtattgtt agattcattg
    25441 agatctctcc taaaaaaggt tgtgaaaaaa gcgtttggcc taaacggcgg gttcgcatga
    25501 ctctcacttc caagtaaatt tctctattcg tatttagaag ctagaatgaa cgcggttacg
    25561 caatcactcc agcctcttct ttatttctct ttattcattt ttttcaaaaa aagattgacg
    25621 ctgatggtat cgattcattc ctaactggat cagttcttca atcaaatcac cgtatttcaa
    25681 gcccatattt tcccataaga gtgggtacat actgaactcc gtaaatcctg gcatagagtt
    25741 taattcattc aggaataatt catttttatt tgtcaaaaag aaatcgcacc ggctcaatcc
    25801 gcttccacct aacatcgtgt aagctaactt cgcgtactct tgcgcttttt gataaacttc
    25861 ttctggcact tcggctggaa tctgcatttc gattttatta ttgatatatt ttgcttcata
    25921 atcatagaat gctacgtctt ttacgacttc accaggcaaa gtcgtccgaa cgtcttcatt
    25981 tcctaataca gcaacttcga tttcgcgcgc ttcaattcct tgttcaacga ttgctcgaga
    26041 atcatactga taggctgttg ctaaagcatt ttgcagctct tctcggtttt ctgcttttgt
    26101 aatgccgaca ctagaaccca tattcgccgg tttgacaaac atcggataaa gcaaagaacc
    26161 ttcacattga tcaaatactt ttttaggatt ttctttccat tgattcttaa gtactggtac
    26221 ataaggaact tgcggcacac cagcagcttg taaaatatac ttggtcatga ttttatccat
    26281 ggcacatgca ctggtcaata cgcctgcgcc gacataaggc atattcaatg tctctaagaa
    26341 gccttggatc gttccatctt ccccgtttgg cccatgtaaa actggaaaaa cgatggctcc
    26401 ttcttctttg atttcgcccg gattgatcac ttttcctgta aagccttcct ctgtctgtcc
    26461 acttgggtcc catgaaagat gcaagacatc tttgctggca ggtttttctg ttagtaatgg
    26521 acctttgacc cattgtcctt cttttgtaat aaatacgagt tgaacttggt aataattata
    26581 ataaatggca tttaaaactg aaaatgcgga aagaatggac acttcatgct ctgcgctgcg
    26641 tccgccatat agtaaagtaa tcttcaaaag attgtcctcc tgtattctaa atcgttcagt
    26701 gatttactct catattttag cacaaaacaa aaataaacga ataacttatt tataaaaaaa
    26761 cgagccagct tctaaaagaa cgagcgtcat ttattttttg tcagctagat tatttgtccc
    26821 gttttttata aaaagttatg ataaagaaaa ggagggatga tgatggcgca aacaaatcca
    26881 cgcagccgct tggcagaaat cattcgagtg ttcatcaatt acaatgtagt gccaaacttt
    26941 gtacagcaaa aaaatcccga acaggtaaaa aaagcctttg aagaactagg ccccaccttc
    27001 atcaagatag gacagatgct ttctgttcga gaagatttgc tttcttctgc cttcactcaa
    27061 acattcaaaa cattacaaga tagtgtacct agcgacacat tttcaacggt caaaaagacg
    27121 atcgaaacag aactttctct ttcattatca gatatattcg atgatttttc aaaatcgcct
    27181 tttgcctctg cttctatggg gcaggctcat cgtgccaagc tgaagaacgg tgacgcggta
    27241 gtcgttaaga tccaacaccc taacatcgcc gaagagatcc gacttgatct tcaactattt
    27301 gaacgagcga tccctttgat caaatacatt cctgaaacta gcgtagttga tctaaaaggg
    27361 gtactccaag aagtaaaacg ttccttgatc aatgaaatgg acttcttaaa agaaagtcaa
    27421 aatggcgaac aattctatca aaagaacaat ggctggaaag agatccgcag ccccaagatt
    27481 tatgacgctt tttgttcgaa aaaagtgatc gtgatggaag aaatgtctgg taaaaacttg
    27541 aatcatttaa tgaacgccga aaacaaaact gaaactttta tcactggaat tcaaaacaag
    27601 cagctgaaac aagaagttgc aaagctttta gttgagaact ttatgaaaca ggtgtttgat
    27661 gatggttttt tccatgctga tcctcatcca gggaatctgc tattccatgt actaacaaaa
    27721 gaagaacaga cacaagctag ccgaaaaact gaaacagtcc atgaaaaaga gttcggttcc
    27781 tttgcttttc gagcctccac ttctgcagaa gaccctgttg cgccatatac catcaactac
    27841 atcgattttg gtatgatggg gcatctttct gcaggactta ggcaaaaact cacacaagcc
    27901 gttttagcgc tgtatacgaa agatgcttat cgaatcgaaa aagcggtcct tcgactttgt
    27961 cagcaagaag gctcgtttga tgaaagtcgg ttccaccaag aactgacaag ttttcttgaa
    28021 cagtattatg attctcctat tgatgaaatc aacttgcaag aagtattcac acatgtcgtg
    28081 acgatttgtc atcaaaataa tctgcagttc gatcgtgata tcacgctttt gctgaaagca
    28141 tttgggacat tagaaggggt gatccgcgtc ctcgatcctg aagtttccct catggaagtc
    28201 gcttcgcctt ttgctcaaca ttattttctc actcatctgg atgtagaaga tacattgaaa
    28261 caaagcggat tagacttatt ggaagggatg aaggctgcgc ctaagatccc tcaacagctc
    28321 caccatctat tagaaatgtg gacttcggga caaggaaaag tcaatttgga attgaaaaag
    28381 caagacaaat tattgagcag gatcgaaagt atgatcaatc ggcttgtttt cggtatgatt
    28441 ttagctgcat tgatcgtagg gtcctccctg cttgtccaag cagcacctgt agaaaacgca
    28501 gaggtcgttt ccttattagg gatatttacg tatgctattg ccgcattcgt cattattttt
    28561 ttggcaatcg atgcattgat ccaaatgtat aagaagagga aaaagtagaa aaaggttgtg
    28621 acagaaagaa tcagcttcaa gaaataagaa ggggttttac ataaattgtt tctcaaattt
    28681 ttgtgaaaat tcggcttatt tttcgaagag gctacttctg aaacaccgag aataaaaaat
    28741 gtgaaaaagc tgttctgcac ccagtaataa gaacaatcaa aaagggaaca tggaacacat
    28801 ttatcctgtt tccctgttcc ctttttgatg tattcgagca taaataaaat accttcgtta
    28861 cctactatct tctgtacttg gtaaacctta gtcaattgcc ttttttaacg cttgacgaaa
    28921 agacatatga tctttctccg taaagggttt tggacccttt gtccttatgc cagcttttct
    28981 taactttcca ctatgataac gttgttctag aagcgtgccg atattcgctt cgtcaaattc
    29041 ttttccagag tgatggaaca ctcgcacacc cttcggcaat acaagtgaag ctaaaccata
    29101 atcttgcgtg atcaaaaagt cattcttctt aaccataccg acgattttat aatccgcact
    29161 atcagctccg cggtctacgt agacgatttg gacatgatta gcgtattctt tggtcgtata
    29221 atggtccaca ctggtcacga taaccacgtc aagaccatag gattctccta attttatgac
    29281 atcttctttt actggagaac catctccatc aatgaatagc ctcattcttt ttcgccttct
    29341 tccataaatg ataaacagtt actaatccgc tcagtaaaag accaacattg atccattttg
    29401 ccaagataag cagttgtaca ctttctatcg gcactgattc atggttcagc gtgatcaata
    29461 atagatggat tggctctccc atttcttttg ctaaaacaaa caaaagcgtc atcaataaaa
    29521 actggtggat cacactccct ttattttgag tagtatactt gacaacgatc cggcgatcca
    29581 ttggaatagc tgtatcagtt tgataatatt ccttatttag ccagctgcct gttactacgt
    29641 gccagaaact aagcagaaag acacctatcg caaaaccatt tggaatcaag aggaccaata
    29701 aagaagaact cagtccaagc aattgcatag ccaatggaga ccatgttaaa cgtttcaaaa
    29761 tccatggtcc gactattttt gctgcaatca agcctgctgc ataaggcaaa tacataaaaa
    29821 tcgaaagttt ttctaatccg tacagtatgc caacataaaa actgctaaat aaaaaaagga
    29881 aattccctag cattccattt agaaaagtaa acagatttag ccagcttggc aacttccatt
    29941 ccttcttctg gtgtcctaaa acaaacacga acaagatgaa cagcagcaag aaaccataaa
    30001 tagcaagatc aaaaagcctt tcatcgaata acaatcgggc atttctcaaa agaaacaata
    30061 agccaaagaa tatcaaaaat aaaacaagct ctcgcgtagc taccagattt cttttgatgt
    30121 ctttgaaatc cagttcgtaa cgcggataat gactaaccgt atggtaggcc atgacgtaaa
    30181 aaagtgtata aatagtaaag cttgctgcaa tctgagtcgg tgcaggcaac tcaatcgctt
    30241 gatacaggag aaatagccag atagctgcaa ataagtactt tttcccatcc atattgataa
    30301 aaccttgcgt cttttcgtga aaattgaccg tggtattagc aggtacaagc catgctgcac
    30361 tcagccctag caaaacactt gaaaaataaa ataaaggaaa ataaaatacg cctaatattc
    30421 caagcaatga accagcacca ccaatcagca gagcgatcat cagcaacgtg taactatcta
    30481 gaccaaatcg tagactgttg actaaaaata gtccagtcat ccgaaaggca taaaacagcg
    30541 taaatggcaa aatcgtcagc caattatgcg agtctgttag tcccaaaaat aaaagataag
    30601 gaataaaaat aaccgtgtta cttaagataa agggcgttgt ttgtgtaaaa ttacgcccaa
    30661 atcgtttgag tttttcgaac atgacaatgg ctccttaaac agtcttataa ctaatctgct
    30721 cttcgatttt agcattaaag gggaaataaa ccaattttac agaatatatt cttgtaaaaa
    30781 aaaatacaga aagaagcata agcttctttc tgcattttta cggtacttga ttactcaagc
    30841 agacaagccc ttctccacaa aagtcttgcc cgacacccct gcatccggca ggtgataaag
    30901 atagaaatca attatcctgt gcctccaaaa tcaattagga gctctgtaaa ccagagtcct
    30961 tctcgacccg tcgcataaat ttactttaac acagttccta tgtttatgcg agagcttttt
    31021 tcattttata atcgaccatt tgaaatcctt gaccgatatc tgaaataagt tcttctgtaa
    31081 tctcaaatcc ttcatgctta taaacctcga ttgcttgatg attcccctga ttcactcgca
    31141 aaaagatctc tctttccttt gtttgcgcag caagttcttc ataccagcga aaaactgaag
    31201 aagtcaatcc ttgaccacgt actttattta gcaaatataa tttacttaaa taaatttttc
    31261 catttatctc ttcataagca gtgtaaccga cagtcttttc ttcgcttttt aataaaaagt
    31321 aggacacacc ttctgtcagt tctttttgaa tctgtttttt tgactgataa gttttcagca
    31381 tgtaatccac ttgtttttga ccaatgatcg gcacaaaaac ttccggccat acttcttgta
    31441 tgactgccat cagctcatcc agttcatttt ctgttacttt tattttttcc atacacccac
    31501 gtcctttttt acattataaa tgaccttgcg cttgatggaa taaaaagagc atgatcatgc
    31561 tatacacacc caccgaccac ggtttcaaga aaatcacaat ctgcataaat tccctaaaag
    31621 ataatttcgt caaaccagct actagacaga tcaagtcgtc tggcgcaaag ggaagcagca
    31681 aggtaacaac tagtactttt ttaatatctt tcgtttttct tgtcagcaat tcatcaaatt
    31741 tctgatactt ttttggcgat aaaataagct ggacaaaggc tacgccatag taacggacaa
    31801 gcaaaaatcc gattgcttcc ccaatcacta ggccaataca actatataaa agcccaaatc
    31861 cattgccaaa catcagcatg cccgctactg aggagatccc tcccggcaaa ataggaatga
    31921 tcacttggat cacttgcaac agaacaaata tcatcactgc atacttgcca aattgctgga
    31981 taaacgcttg caaagacgtg ctcgatcgaa aaatcccatg atgataagca taaagaatca
    32041 acacaaagaa aaaaaacaga ccaataatgg gcatgatctg aagacatttt tgtaaaactg
    32101 atcctttttt agacatctat attccccaca tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DACDTO010000023        31395 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDTO010000023 DACDTO010000000
VERSION     DACDTO010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31395
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.023"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..396)
                     /locus_tag="IWK70_001122"
     CDS             complement(<1..396)
                     /locus_tag="IWK70_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9613686.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQ"
     gene            complement(472..618)
                     /locus_tag="IWK70_001123"
     CDS             complement(472..618)
                     /locus_tag="IWK70_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9613687.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(722..1033)
                     /locus_tag="IWK70_001124"
     CDS             complement(722..1033)
                     /locus_tag="IWK70_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9613688.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(1035..1232)
                     /locus_tag="IWK70_001125"
     CDS             complement(1035..1232)
                     /locus_tag="IWK70_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9613689.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1626..3353)
                     /locus_tag="IWK70_001126"
     CDS             complement(1626..3353)
                     /locus_tag="IWK70_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9613690.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(3346..4539)
                     /locus_tag="IWK70_001127"
     CDS             complement(3346..4539)
                     /locus_tag="IWK70_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9613691.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4726..5370)
                     /locus_tag="IWK70_001128"
     CDS             complement(4726..5370)
                     /locus_tag="IWK70_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9613692.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(5464..5598)
                     /locus_tag="IWK70_001129"
     CDS             complement(5464..5598)
                     /locus_tag="IWK70_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9613693.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5600..7732)
                     /gene="feoB"
                     /locus_tag="IWK70_001130"
     CDS             complement(5600..7732)
                     /gene="feoB"
                     /locus_tag="IWK70_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9613694.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7729..8202)
                     /locus_tag="IWK70_001131"
     CDS             complement(7729..8202)
                     /locus_tag="IWK70_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9613695.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8603..8827
                     /gene="nrdH"
                     /locus_tag="IWK70_001132"
     CDS             8603..8827
                     /gene="nrdH"
                     /locus_tag="IWK70_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9613696.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8986..11145
                     /gene="nrdE"
                     /locus_tag="IWK70_001133"
     CDS             8986..11145
                     /gene="nrdE"
                     /locus_tag="IWK70_001133"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9613697.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            11195..12160
                     /gene="nrdF"
                     /locus_tag="IWK70_001134"
     CDS             11195..12160
                     /gene="nrdF"
                     /locus_tag="IWK70_001134"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9613698.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(12249..13388)
                     /locus_tag="IWK70_001135"
     CDS             complement(12249..13388)
                     /locus_tag="IWK70_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9613699.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13697..14410)
                     /locus_tag="IWK70_001136"
     CDS             complement(13697..14410)
                     /locus_tag="IWK70_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9613700.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14664..15365)
                     /gene="nagB"
                     /locus_tag="IWK70_001137"
     CDS             complement(14664..15365)
                     /gene="nagB"
                     /locus_tag="IWK70_001137"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9613701.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15599..17131
                     /locus_tag="IWK70_001138"
     CDS             15599..17131
                     /locus_tag="IWK70_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9613702.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(17322..18524)
                     /locus_tag="IWK70_001139"
     CDS             complement(17322..18524)
                     /locus_tag="IWK70_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9613703.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(18606..19295)
                     /locus_tag="IWK70_001140"
     CDS             complement(18606..19295)
                     /locus_tag="IWK70_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9613704.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(19401..19769)
                     /gene="rplL"
                     /locus_tag="IWK70_001141"
     CDS             complement(19401..19769)
                     /gene="rplL"
                     /locus_tag="IWK70_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9613705.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19822..20325)
                     /locus_tag="IWK70_001142"
     CDS             complement(19822..20325)
                     /locus_tag="IWK70_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9613706.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(20735..21424)
                     /gene="rplA"
                     /locus_tag="IWK70_001143"
     CDS             complement(20735..21424)
                     /gene="rplA"
                     /locus_tag="IWK70_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9613707.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(21528..21950)
                     /gene="rplK"
                     /locus_tag="IWK70_001144"
     CDS             complement(21528..21950)
                     /gene="rplK"
                     /locus_tag="IWK70_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9613708.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            22293..22961
                     /gene="sdaAB"
                     /locus_tag="IWK70_001145"
     CDS             22293..22961
                     /gene="sdaAB"
                     /locus_tag="IWK70_001145"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9613709.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22974..23846
                     /gene="sdaAA"
                     /locus_tag="IWK70_001146"
     CDS             22974..23846
                     /gene="sdaAA"
                     /locus_tag="IWK70_001146"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9613710.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23937..24956)
                     /locus_tag="IWK70_001147"
     CDS             complement(23937..24956)
                     /locus_tag="IWK70_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9613711.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(25129..26016)
                     /locus_tag="IWK70_001148"
     CDS             complement(25129..26016)
                     /locus_tag="IWK70_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9613712.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(26093..26638)
                     /gene="nusG"
                     /locus_tag="IWK70_001149"
     CDS             complement(26093..26638)
                     /gene="nusG"
                     /locus_tag="IWK70_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9613713.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(26739..26909)
                     /gene="secE"
                     /locus_tag="IWK70_001150"
     CDS             complement(26739..26909)
                     /gene="secE"
                     /locus_tag="IWK70_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9613714.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26933..27085)
                     /gene="rpmG"
                     /locus_tag="IWK70_001151"
     CDS             complement(26933..27085)
                     /gene="rpmG"
                     /locus_tag="IWK70_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9613715.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            27247..27891
                     /locus_tag="IWK70_001152"
     CDS             27247..27891
                     /locus_tag="IWK70_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9613716.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27947..28450)
                     /locus_tag="IWK70_001153"
     CDS             complement(27947..28450)
                     /locus_tag="IWK70_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9613717.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(28463..28509)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            29047..30012
                     /locus_tag="IWK70_001154"
     CDS             29047..30012
                     /locus_tag="IWK70_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9613718.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(30111..31283)
                     /locus_tag="IWK70_001155"
     CDS             complement(30111..31283)
                     /locus_tag="IWK70_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613719.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(31270..>31395)
                     /locus_tag="IWK70_001156"
     CDS             complement(31270..>31395)
                     /locus_tag="IWK70_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613720.1"
                     /translation="KLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT         9637 a         6414 c         5493 g         9851 t
ORIGIN      
        1 ctgttcaaca atcctattta tagctaattt tctatataac caaataaaca tcagcaaaaa
       61 caaagaatca agcattatct ggaatactac ataaccatac ccttttatcc cttcaaatga
      121 aaacatatac gtcataacca tggaagtaat aatagatctg aaatcatcaa taagcataca
      181 taatagtaat ttatttaaaa gcactatatt agttttcttc ttttccttta atataaaata
      241 acttccgatt ataggtatag ctgcggaaac atcagtaaat aattcaacaa aaaaagtgag
      301 tggtatcaat aaacatacaa aaagatattt tcttttgtta agatcagtat tagtcatata
      361 aaataagaga aaaaagaaaa agtttcccac gaacattctt aatagataag cttttgtatc
      421 gatagtaatc aagagatccc ctcctgattt taggatatca taaaaaaaat tttagttaca
      481 ctttttaaat aatgaaaaaa cacattccac taaatttgat gccggttttc cttgaggttt
      541 tgttccagca cctcctttaa tttttttaag ctcttgatct gaacattgtt tagcatttag
      601 tctatttttt tcttccatta cgattccccc tacaaactat aaatactttt atgtcactga
      661 ttcaatatac ctatttttct aatcaaaaaa ttttgagaca tatttaatat tataagtatc
      721 attaaaattg agatttatct ccataatctg ctcgatataa accatttatt ccagcagttc
      781 ttccaatttc actcaaattt cttattaatt cctcttgata ttcagtgaat cttattttat
      841 tagtataagc agtaaaatat agataattca ccaatctatt tattaaagga ccgggatcta
      901 attcatattt ttgtttttct aattttaaat ataccttttg taaaacctca agaatatcag
      961 aatatttagg atctttactt atagatatat cattatataa ttcatgaaca atttgcttag
     1021 catttttttt cattttagca cttccctgga attgctccac ctaaaaaacc acctatagac
     1081 attcctgcaa tacaagtagt tgccttggcc caatcgaccg tacatttatt tttagtgcaa
     1141 tacactccat ttccataata ttttccacta tgagtggtac ccccatagat aagttgtgtc
     1201 tctttaatag acaaaatttt taaatgtttc ataatctaat cacccctaaa ttaatgtttt
     1261 tagactatta ttctaacatt cattaaattt caaaaaacaa ttccttcttg aaacgtagca
     1321 aaataattcc tgaataagaa tttaatcact tttaagtata taaactatgt atattcttta
     1381 atattttttg agaatgcact tcgtacataa taaactttaa tcacattagt ctttcatacc
     1441 tacgatcgat ctctttgact cgcacccata aattcataat agtttgtcgg cgtctttgga
     1501 cgtttcgaag atgagctacg cattctcttt ttttatagac aaaaaagata gagacgaaca
     1561 tggagcttcc aaatttggat atttgatcca attttggaag ctccatttat ttcgtgtctg
     1621 tctttttatt ttttccagct ttcgactaat tcttggttct ctttgatcca gtcttttgct
     1681 gcttgctgtg gatcttttcc attgttgatt tccaacataa ctttttccat atctttttct
     1741 gaccaatgga aattgtctaa tactttgtaa gcttctggct ggtcttcttt caatcctttt
     1801 cgaaccatcg tatggatctg ctcttcgctt cccattccgt tttcaggatc ttctaagtat
     1861 ttcagatcat atttggcaaa catccaatga ggtgtccatc ctgtaactac aattggttcg
     1921 tgttttttga tcgcttgacc tagtgcaacg gtcattgctc ctgaagaaga tgtctctact
     1981 ttccagtctt tcaagttgtc atatttttgg atcgtgtttt cagcagccgt aactacacca
     2041 gctcctggct cgattcctat gattttctta cctgcctgat cggttaactc atcgatcgaa
     2101 tttacatcca tgtaggctgg cactgctaaa ccaacttttg ctcctgtcaa attggcacct
     2161 aggtcttcga cttgatcttt atattgtgca tattgtgaac catgagtttt aggtagccaa
     2221 gctgaaacca tggcatctga ctcgccattt gaaacagatt tccacatgat tgaattatct
     2281 aatggcgtca tcgttacatc atagcccatt tgtttcaata cttcaccgac tacatttgtt
     2341 gaagcaactt ctgtatccca ttcaacataa gataagttga tacgttttgt ttctttggca
     2401 gatgaaaaac tccctatacc aatcgcacct aagatcacta ctactacagc accaatgatc
     2461 agtccttgac gttttttact tttctgtgtt gttgctcctg cagctttttt attgtttggc
     2521 tggttcaaat gctgtgtaaa acggtcaatg atgattgcta agataactaa tgcaaccccg
     2581 ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg ataatacgcc gcgtcctaag
     2641 ccgggtgcac cgatcataga ggcaataacg accattgaaa gtgacagcat tgttgtttga
     2701 ttgatccctg ccatgatcgt acttttagct aaaggcaatt ctagtttgaa tagtttttga
     2761 cgacttgtgc tcccgaatga atcagaagct tctactaatt ctttaggaac ttgtcgaatc
     2821 cctaaattcg taaaacgaac ggttggtggc agagcaaaga tgactgaggc aaatacccct
     2881 ggaaccatcc cgataccgaa aaatgcaact gctggaatca aataaacgaa tcctggcatt
     2941 gtctgcataa agtcaaggat cggtttgata atactttggg ctttactgct ttttgccatc
     3001 aagatcccta atggtacccc aatgatgatc gagatcacac tagataaaag aacaagtgtg
     3061 acagtattca tcaaatcatt ccatagacct tggttataaa taaaccatag accgattaat
     3121 gtgaacaacg taagtcctgg acgtttctta gaaataaaga aagctgctac cgtcaataac
     3181 aagataaaca aaggagctgg aatcactaac agtaaatttg taatccccga catcactgcc
     3241 tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag ttgtcgtaaa ccattctgtg
     3301 atattttcta cccagtttgc cactggtaat tgatagttca atagattatt catgttcact
     3361 cacctcgctt gtttctgcta atgcttcaat cacactacct cggatgacga caccttttaa
     3421 ttttccatcc tctacaactg caagtggagt cggtgagtca taaataatat tgaaaatatc
     3481 acttaccacc atgtctttcc caatcaccgt cacattttca tctaggaagt cgatcaatgg
     3541 acgtttttgc ttcctagctt ctaacgcttg atctgcagtg atgatccctt tcaggtgtct
     3601 ctttctgtca acagccatca gcatgctgac ttcttcatta cgcatccttg tcaatgcaac
     3661 gtttggtcca tcagattcga tattcgtcgt caatgcaggt accatgatgt tttgagccgt
     3721 aagcactttg gaacgatcga cttcttccac aaattcacgt acataatcat ttgctggatt
     3781 ggtcaatatt tcttcacctg ttccgatctg catgatttcg ccgtctttca tcaaggcgat
     3841 tctgtcccca atccgtaaag cttcattcaa atcatgggta atgaaaataa tcgttttttg
     3901 aacattggct tgcaggtcca gtaattcgtc ttgcatttct cgccggatca gcggatcaag
     3961 agccgagaac gcttcgtcca tcagcaagat ctcaggatca tttgctaaag ctctggctag
     4021 gccaacccgt tgttgcatcc caccagataa ctgactagga tattggtctt taaatgaaag
     4081 caaactagaa ttttctaatg ctttttctgc tttttcttgt cgttcttctt taggtacacc
     4141 acggacttct aacccgtatt ctgtattttc caagatcgtt cgatgtggaa acagaccgaa
     4201 gttttggaaa accatattga ttttatgacg acggacttct cgcaattctt ctttgcttaa
     4261 cttcgataca tcttctccat caatatagat actgcccgaa gttggctcaa tcagacgatt
     4321 caatagacgg atcaatgtgg atttcccact cccggaaagc cccatgatca cgaagatttc
     4381 tccttctttt acgtcaaaat tcacgtcata cacaccaacc gttgcaccgg ttttctttaa
     4441 aatctccgtt ttcggctggt gatcattcat catatctaaa gcggcttgtg tctttttacc
     4501 aaatatcttt gttaaatgtt ttacttctac ttttaccaaa caaattctcc ttttttatgt
     4561 aaattcattt ttctttctga aaaaagtgac cacactatgt taacatcatg tagtcacttt
     4621 gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa acttggctaa atctttgtaa
     4681 ataaaaggtt tagccaagtt aatatttttt taatatttca cacaatcatt ctgcaagctg
     4741 atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta tattcatttc caataaaatg
     4801 aacgatgtcc cctgccgtaa atttagcata ggggccaggt gaaacaagca gttcttcatt
     4861 atgagaaatt gcgataactg tggcgccagt cgcctgccag atattcaact cgctaattga
     4921 acggtccaga tgcattgctt tttctgataa agataattca aacggaagta aaggattcat
     4981 cttatgtatt cgcttcgttt gattgaccag ctgatccaat aattgagaaa aatgattcag
     5041 ctcatcttgt tgcttctcca cacttgccat gatttctgct tttgtatctt ggatggattg
     5101 tacatcttga tattgcaata ggaaatcttt tgctttttcc ttcgaagcaa caaatgcccc
     5161 acttccatgc cgtacttcca tgattccaag atctaccagc acattgatag ccttgcgggt
     5221 agtctcagga gaagcattgt acgtactggc aagtgttgag cgggcatgga tcttctcacc
     5281 cactttatat cggttttcaa cgatacgttc ggctaaatca acagcaatct gctggtagcg
     5341 cgggcggacg atttttttct ttccagacat tttctttcct ctttcttttt ttactttccc
     5401 tcatcttact atgctctagc aaaataagca aaaaagggga gaatagcctc ttgcaacgac
     5461 tgatcatcgc ttgatctgtt ccttttttac tgggcaagct gtttgacagt catcacaatt
     5521 cttaccggat tttaaacgag tatagacaat tgcgcctgca cttccaaaga ttaacacact
     5581 taataagaat gtcgccatcc tattcctctc cttctaatga caatatagta taaaactggt
     5641 tcttggattt tggctgacgg aacaaaacat aaacgagtcc tatcatcagt aaaatagcaa
     5701 gatacgtccc taacgctacc tgtccaccct ccaataagtg tcccaactga taaatcacaa
     5761 aactaactaa ataagctaaa ccgcattgat agcctatggc gatccaagtc catttcttgg
     5821 cattcatttc ccgatggata gccccaattg ccgcaaaaca aggcgcacaa agtaaattga
     5881 aggctaacaa tgaataagct gctgcgggag tatatgtgtg ttgtagcact tgccagattt
     5941 ctctgccatt ttcggatact tccgcatgac caaacaaaat accaaatgtg ccaataatat
     6001 tttctttagc gaccaatcct gtaatcgctg cgactgttcc ttgccagttt ccccaaccaa
     6061 gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc taagatactt tggtcttcag
     6121 aaacaggttg agcataaaaa ttataagaag agctgaacca gatcacgata ctagaaacaa
     6181 agataatcgt tcctgctttt ttaacaaaag acttgctgcg gtcataggtt tgtcttagga
     6241 ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat gataaaagga gctgtatctc
     6301 cagcaaacaa acgtgttttt ttcaagctga ttccagagaa aataattgca gtcataccaa
     6361 gaaaataggc agaaggagct acccaagatt gatgaggaaa aaaggcccct gctactaagg
     6421 caataatcgg caactttgca gaacaaggca tgaaggttgt gaccatgatc gtcaaacgac
     6481 gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc tggcactcca catcctgtag
     6541 caatcagcat aggaatgaag gattttcctg aaagaccgaa tttcctgaaa agtcgatcca
     6601 tgacaaaagc gatcctcgcc atatatccgc agtcttccag aaagctcaag cataaaaaca
     6661 atataatcaa ttgcggtaga aatcctaaca ctgctcccac gcctgcgatg atgccgttca
     6721 agatcagctc ctgcatccat cccgctactt gccattcagc taacaatctg ccaacatgtt
     6781 ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc tgtccccatt gttcctactg
     6841 tttgaatagc cagataatag atcagccaca tcacaaatgc gaagatcggc aaggctaacc
     6901 aacgattcgt tgcaatctga tcgattttgt cactcatcga taattggaaa gaatcatttg
     6961 acacaacaca gagtgtacat agttgggtaa taaattcata acgttcattg accagaatcg
     7021 tctcactatc gtcgtccaat agtttctctg ttaactggat cagttgctcg atctcttttt
     7081 gctgagaaac tgctatctcg taatcctctc ttaccttctg atctcgttca aaatatttca
     7141 ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac atcttcaatt tcatgtaaag
     7201 ctgcttctaa acgtggatca tatgtacagg gaacaggaat agaaggccgc ttttttgcag
     7261 actcctgaat cttttcgatt agtttttcga ttccttgctt tttcaatacg ctgattggta
     7321 aaactggtac accaagacca taggaaagct tttctagatt gatttttttt ccttcttttt
     7381 tgatgacatc catcatattc agaccaatga tcataggaat gtctaaatcc aataactgcg
     7441 tagttaaata aagatttcgt tctaaattgg tagcatcaat aatgttgatg atcagttcag
     7501 gctgttcctg aaccaaataa tctcttgcaa tcacttcttc tggtgtataa ggagacaaag
     7561 aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt ttttttcaac gatccttctt
     7621 ttttttctac tgttacgccc ggccaattcc ctacatattg attcgaacca gtcaagagat
     7681 tgaacaagct agttttcccg ctgtttggat ttccgactaa cgcgatattc attctttttc
     7741 ctccctttgt atcaagatta gttcggcctc tgactttcgt aacgtcagtt cataccctcg
     7801 cactgtcagt tcgattggat ctcccagagg ggctagtttt cggataacta tttttacatt
     7861 tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg ccttgtccgt ggattgctac
     7921 gacgatccca gtctctcctg cttttaattg atcaagagaa gtccagcttt cagaggaagc
     7981 agtcttttta ttgacaaaga tttgacctag tatttcccga cttactgcca ctcgatgact
     8041 ttttaccagt aaaatggcat tgttgcctga taaattgacg acgacgattt tacttccggc
     8101 aacgatcccg atattttgca aatggcgttt tgttttttct tctgtttcga ttcgttcgac
     8161 ttgataaacg aaattctgct ttgcctgcgc taaggtctgc atattgattc ctctcttgtg
     8221 aaataaatca tgtattaaaa tcattcatta tatgttctaa ttttcttacg tgttacgcaa
     8281 ataaatgaga atcattctca catagaatac tctcggtaat tgagaatgtc aattatactt
     8341 gtgatattat gagcgaatct ttagtattga tagataaaaa tgagaaaaaa atatttcttc
     8401 atagttttcc acaatttttt ctctattaaa tttgctattt tcccacacga tacttgtttt
     8461 cagctctttt ttcaaaaaca caaaacttag tattccacag tttccacaac acaatatgta
     8521 gtgtttgttg tttgactttt ttactaagag ttttatacta tctattatag ataataagaa
     8581 tgtgttagag gaggcactca ccatgaacgt aaaaatcttt tctaaaaata attgtatgca
     8641 atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt gcttttgaag aagtcaatat
     8701 cgatgaacag cctgaagcag tagactggtt gaaagcacaa ggattccaaa gtgttcctgt
     8761 cattacttct gatgcagaaa cagttatcgg attccgtcct gatcagttgc gtaaacttgc
     8821 cagctaacat gactaaaaga atcaaaacgt gatacatgga tttgaagggc ttgagacagc
     8881 accagttttt gtcgcaaact tccaaatcct tttttaaagc ttaaaaacaa tgcaatgatt
     8941 tgcaatcaag catcatcaaa tacgaaatga aggttgggcg tacgtatgag tcttaaaaat
     9001 ttacaagatg tcagttattt caagctgaat aatgaaatca atcggccagt caatggtcaa
     9061 atccctttga ataaggataa agaagcttta gttgcctttt ttgaggaaaa tgtcaaacca
     9121 aatacgatga cttttctatc tattatggaa aaaatcaatt acttgatcga tcaagaatat
     9181 atagaagcag aattcattca attgtattca ccatcattta ttgaaaagct atatcaattt
     9241 ttggacgagc aacatttttc tttcaaatct tttatggctg cttacaaatt ttattctcaa
     9301 tatgcgttga aaaccaacga cggttccgct tatctggaaa cttatgaaga tcgagtagcc
     9361 ttcaatgcgc tttactttgc gaacggggat gaagaattag cacttgctct tgcagacgaa
     9421 atgatccatc aacgatatca gccagctaca ccttctttct tgaatgctgg acgtaaacgt
     9481 cggggcgaat tggtgtcttg tttcttgact caagtcactg acgatatgaa ctctatcggg
     9541 cgctcaatca actctgcttt gcaattgtcc cgtattggtg gcggcgtagg gattacttta
     9601 tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg agggtgctgc aagtggtgtc
     9661 gttccagtca tgaaattatt cgaagatagt ttcagctatt ctaatcaatt aggtcagcgt
     9721 caaggagctg gcgtggttta tttgaacgtt ttccatccag acattattgc tttcctttca
     9781 gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa ctctttctct tggcgtgatc
     9841 gtacctgata aattttatga attgacacgt aaaaacgaag atatgtattt atttagtcca
     9901 tatagtgtcg aaaaagaata tggtgtccca ttctcttatg taaatattac agctgagtat
     9961 gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa tcaaagcgcg tgatcttgaa
    10021 aatgaaatct caaaattaca acaagaatct ggctatcctt acgtaatcaa tattgataca
    10081 gcgaaccgcg agaatccaat cgatggcaag atcatcatga gtaatttatg ttcagagatt
    10141 ttacaagttc aaacgccttc tgtgattaac ggcaaacagg aatatgaagt attaggaacg
    10201 gatatctcat gtaacttagg ctcgacaaat atcgtgaatt tgatgcaaag ccctaacttt
    10261 ggtcaatctg tgcgtacgat gactcgtgca ttgacattta ttacagatgc atctgatatc
    10321 gatgtcgtac caccgatcca aaacggaaat aagctgaatc atacgattgg attaggtgcc
    10381 atgggcttac acacttatct tgcaaaagaa caaatggaat atggctcaga ggattcatta
    10441 gactttacaa atatttactt catgctgttg aattattgga cacttgttga aagcaacaat
    10501 atcgctcgtg aaagaaaaca agttttccat aattttgaaa aatcagctta tgctgacggc
    10561 acttatttcg aaaaatacgt aactggagaa ttccagccaa aatcagaaaa agtcaaagca
    10621 ttgtttgaag gcatttttat cccaactgct gaagattgga atgctttgaa acaagcagta
    10681 atgaaagatg gactgtatca tcaaaatcgc ttggcagtag caccaaatgg ctcaatttca
    10741 tatatcaacg atacgagtgc aagtattcat cctatcactc gcatgatcga agaacgccaa
    10801 gagaagaaaa tcggtaaaat ttattaccca gcgccttatc tatccaatga gacgattccg
    10861 tattacactt ctgcttatga tatggacatg cgtaaagtaa tcgatgttta tgcaatggca
    10921 caacaacacg tcgatcaagg catgagtttg actttattca tgcgttctga aatcccagaa
    10981 ggcatttatg aatggaagac aacaacgaag caaacgacac gtgatttgaa tattttaaga
    11041 cattacgctt tccacaaagg aatcaaatcc atttattacg tacgtacctt tactgatgat
    11101 gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga tctaatatca tagatagatt
    11161 cgcttattca tattttaatt aaaggagcat tctcatgtca gaaacatatt atgaagcaat
    11221 aaactggaat gaaattgaag atatcattga taaatccact tgggagaagc tgacagagca
    11281 attttggcta gatacacgta tcccactttc aaatgaccta gatgattggc gtacattgtc
    11341 tcaattggaa aaagatacag taggacatgt atttggcggt ttgacactgc tagatactgt
    11401 ccaatctgaa agtggcatgg atcagttgcg taaagatgtc cgcactcccc atgaagaagc
    11461 cgttttgaac aatattcaat tcatggaatc cgttcatgcc aaaagttatt cttctatttt
    11521 tagtacactg aatacgaaaa aagaaatcga agaaattttt gactggacaa atacgaatcc
    11581 ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac aaacacggta caccgctaga
    11641 aaagaaaatt gccagcgtct ttttagaaac attcttgttc tattcaggtt tttatactcc
    11701 tctttattat ttaggaaaca acaaattggc taacgtagca gagatcatca aactaatcat
    11761 ccgagatgaa tctgtacatg gaacctacat cggttacaaa ttccaattag gattcaacga
    11821 gttacctgaa gcagaacaag aaacgttaaa agactggatg tacaatcttc tttatgagct
    11881 ttacgaaaac gaagaacgtt atacagaaga attatacgat ccaatcggct ggacagaaga
    11941 agtcaagaca ttcctacgct ataatgccaa caaagcattg atgaacctag ggatggatcc
    12001 actctttcca gatactgcca atgatgtcaa cccaatcgta atgaacggga tttcaacagg
    12061 cacaagcaac catgacttct tctcacaagt cggaaacggc tatttgttag gtcatgtaga
    12121 agcgatgaaa gacgacgatt atttgattgg attggaataa ggatttattt taattcaaaa
    12181 atttttcgaa aaataaaaag agtttagcgt acagatattt taaatgtaca ctgaactctt
    12241 ttttatgctt attcacttgt ttcttccagc acttttggac tgtctaactt ttccgaaaca
    12301 tgttccattc ttctcaataa atagattttc cggatattta atattatcgt cttaatcact
    12361 gcatagacag gtatgcaaat cagcatgcct aatattcctg cgacacttcc tgctccaatc
    12421 aataccaaga tgatcgtcaa aggatggata ttcattgatt tcccaaataa caacggcttg
    12481 atcaaatttc catctaattg ttggatcact aaaatcgaca acgccatata caaagcttga
    12541 aatggagaga taaacaaacc gacaatgatt gctggtaccg ctccaataaa agggccgaca
    12601 tacgggataa tattcgtgat tccgcaaatg accgcaagca acaagctata aggctgatcg
    12661 aatattctca ttgccaaaaa gctcattaca ccaataatca aagcatctaa gatcgtactg
    12721 ctgatatagg cagataatgt ttcgtttaat tcttttaccg tttggcgtaa ctctttacga
    12781 atcgctttcg gaaagaagtg agacaacgca tcaataaatt tgtgcccatc cttgaacata
    12841 aatagcaaaa taaatggaac ggtaaataac aaaataaaaa attgcatcat aaatgaaatg
    12901 atttttgaga cacttgcaga taaactgact aatatgatat tgaacaaatt actaagagat
    12961 aaatttgctg atgctaattg ttcttccaaa ttgaaatttt taaactcttc ccgctgcgcc
    13021 aattcattta accatttgct tgtttcatct gcatacattg gcaagctctg ggtcaattga
    13081 attgtctgtt ccattaactg aggcactaca ttcatgacag ttaaaacaac taaaatgatt
    13141 acgaccaaaa acgtcaataa aaaaccgaac actctcggga tccgatgctt ttcgagaaaa
    13201 acaacgattg gatcgaacat ataatagata aaaccagcaa caagcaatgg taaaaaaact
    13261 gccgaaagca ttttcaatat tggactaaaa atattcggca tttgtaaaat aaagtacacg
    13321 ccgatgatcg tgagtactag ctccactgtc cagaaaaata attttgattg tttaaaacgt
    13381 gattgcatac atctcctcca aggtcctttg atacttctat tctatcattt aacaaagagt
    13441 tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa aataaacata ttttaatgag
    13501 tagacgagta cttaaacaaa aacacggttt ggagtagtga caaaaattat actgcagtcc
    13561 ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg taataagccc agccaaacga
    13621 aaaatataag gaactatttt tatttggctg ggcttattac ttgatgtagg acatctattt
    13681 tacaacctca gatcatttat tgcgcaatac tgactaaatg caactttgtg atatttttgt
    13741 gtacggacaa ctcctcgatc acatcggcat aagtgagccc ttttggaaga tcaatcgtat
    13801 agatattttt gtacactcgg tagtcatcaa cgaattttac gtcaaaattc acatcttcga
    13861 ttacgatatt tttctcttca aaataatcgg taagaaaatc tttggtttct tttctatgga
    13921 taaattggat ttctaacttt ttagtcgtag gtacatggat cacgcgtttc actactgtga
    13981 gtgcaatagc aatcccgata aaactgatga tcgcaatgga atagtaaccc atgccgatgg
    14041 cgatccctaa tccagcaatt gcccataaag aagcggcagt cgtaagtccc gtcaccgatt
    14101 gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac accactcacc acttgagcaa
    14161 tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg tggatactct aaagcatctc
    14221 ttaatgcacc actagcgatc tcaacttgga tcaatgcaat gatacatgct cccatacaaa
    14281 ctaaaatatg cgtacgcata cctgctggac gatttttata ttgtcgttca aaaccgattg
    14341 ctccgccaaa tagcatagcg atccctaaac gtaaaataat ctctggaatt gtcaatacca
    14401 tatcattcat ctaaacaccc tccagtccaa tatatcatgt ctgaaaggag cagacaaaaa
    14461 aagtgcaatt tttacctctt acgaaaaaag gactgtgaca aaatttttgt catagtcctt
    14521 tttatgaata aacggtgttc aatcagctga ccctcggtat tactcaacaa acggcaaaat
    14581 atggggagct atttttcctt tgtcttctct aatactcagg tcaaaacgct gatttcacaa
    14641 cctctttagc ctactattga aaattacaac ttacttgctg cttcttcatc gatgatcaca
    14701 acaacattgt cgtgattttg caatacactt gctggcaaat gattcgttac aggtccttca
    14761 atcatccctt tgattgcatc agctttgctt tcgccaaatg ccatcaagac gatttcttta
    14821 cttttcataa ttgaaccgat tcccattgaa acagcttttg ttggtacatc ttctgcttta
    14881 tcgaagaatc gtttgtttgc attgattgtt gattctgtta agtcaactac tgaagtcgtt
    14941 ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc cattttgccc gattcctaag
    15001 atttggatat cgattggatg tgcatcgatg atttgatcat actttttgca tgctgcttcc
    15061 agatcttctg cttttccatc tggtacaaac gtttctttga atggtttttt atcaaataat
    15121 tggacatcca tgaaatgacg gtagctttgt tcatctgaac cgcctagtcc aacgtactca
    15181 tctaagttta cagaagtcat ttcactgaaa tccaaatcgc ttgaagtcat ttctttatat
    15241 aaagtaactg gtgtgcttcc tgttgccaat cctaatactt ttgcaccttt gttcatacct
    15301 tctttgatga tttcaaatgc tttttttccg ccttcttcag cgttttttac acgaatgatt
    15361 tccatagtga ttgctccttt atattttggt atagcccaat tatagcattc tttttttctt
    15421 tttacaataa ttagtataga ccaaattgca tttttttcat tttcttgaca ataaatagga
    15481 atccgtgtta ggtttcataa gatggttcaa tcaaacaatc catgttgcat gaaaaccaag
    15541 caagtgattc actgtggacg ctttatgtct gcagtttttt tatgaaaaag gagttattat
    15601 gagtatactg acattaaaca acattaccca gcaatttggc gacaaaatcc tctatgaagg
    15661 tgtcgatctg caattaaatg ctggcgaaca tcttgggctg attggacaaa acggagccgg
    15721 taaaagtaca ttgattaaaa tcatcactgg tgagatctta ccagatgacg gacagatcca
    15781 ttggcaaaaa aatattcaca aaggttacct cgatcaatat gtggaagtcg atgaaacatt
    15841 aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa tacgaaaagg aagaaaaaat
    15901 tgcttttctt taccaaacat atagcgagac cttcgatgat tccttgctgg aaaaagctgg
    15961 gaaactccaa acggaattag accaaggagt gttttatcaa atcgacacct tagttgctga
    16021 gatgagcagc gggttgggga tcgatgtctt aggtttagat tcactgttga aaaatctaag
    16081 tggtggacaa cgttcaaagg tgattttagc aaaactactt ttagaaaacc cagatgttct
    16141 gatcttagat gaaccaacca atcacttaga tgatcagcat atccaatggc tgacgcagtt
    16201 tcttcaggat tttgacggtg cgtatatcgt gatttcccat gaccaagaat ttcttgatca
    16261 gatcaccacc catatcgcag acatcgagtt tggaaagatt actaagtata ctggccatct
    16321 aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc tatttgcgcc aatatcatgc
    16381 tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga aaatacaaag ccggttcccg
    16441 atcaacgatg gcaaaaagcc gacaaaaaca attagataaa atcgaacggc tgacgcctcc
    16501 agcttcagca agtaaagctg tctttgattt tccattctcg cctattgtca cgacattagc
    16561 agtggaaaca actgatttag tcatcggata tgatcgaccg ctacttgcac ccatcaattt
    16621 gaccatccgc tttggtgaaa atattgccat tcgaggcttc aacgggattg gaaaatctac
    16681 tttattaaaa acactgatcg gtgagattga aaaaataagt ggcgatttcc acttcccaga
    16741 taacacaaaa atcaattact tttcacaaga tctgtactgg gaaaatccat tagaaacacc
    16801 actacaatat ttaagcagtc aatttccaaa agcgactatc aaagaattac gtcgccaact
    16861 agctaaagca gggttagtta atcaattagc aagtgaacct ctcactacat taagtggtgg
    16921 ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac caaggtaatt tactgattct
    16981 cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa agcttacaag acgggataca
    17041 gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa gaattttatc aagatcttgt
    17101 gcaacgagtc attgagattg aaccaaaata aacgtttgat tgaatagttg aaaaaaagga
    17161 gctgtgacaa aagttttgtc acaactcctt cttctgaata aatggtggcc aattagctga
    17221 ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc tatttttatt tggctattct
    17281 tattacttga tgcagaacag cttttcacaa cctcttttcg tctatttttt attttcgaga
    17341 tttctctttc ctttaaaaac aactgtttga aagaagaacg cacatccttg aaaaacaagc
    17401 ccgctaaccg ttacacctaa ccagccatac tgctgccaca ttgtcgtccc tagcaatgaa
    17461 ccagccgcac ctccaataaa gtacataaac ataaatacag tattatttcg gttgcttgct
    17521 gcttcaccta gcccttgtac tcgtgtttga ttggcgactt gaccaaattg attcccgacg
    17581 tctaataaaa taatagctaa aagcaaaatg gccaaatggc ttccgccaac caataaagca
    17641 ataaagctta ttgtttgcat aacaagtccc atatacacga tttttctttc agaataagaa
    17701 tctgccagtc gtcccacgat aggtgcagca actgctccag acaaaccaaa aatcgctaat
    17761 atccctgctt cgaatgttcc ccaattgtag gcgggactgc tgatataaaa aatcaacgta
    17821 gaccaaaaga tcgaaaatgt tccaaacatg aaaaatccac tgactgccgc ttctcttaat
    17881 agacgttgag aagcaactaa atgcggcaaa cttttaagtg aatcaaggta tgaaacagct
    17941 gtactacttt gctggacaga acgcggcatt ttccagtaaa gcagaagtgt aagaattccg
    18001 actgcacata aagcaaataa atagatcatc cgccaagaaa aaagactggc aacaatacca
    18061 gataccgtcc gagaaagcaa aatccccacg agtaaaccgc taagcaatgt acccatgacc
    18121 ttccctcttg cttttggtcc cgctagtacc gcaccgtaag gaatgatgat ttgcggcacg
    18181 atcgaaagta atcctattaa aaaagaagcg catgcaaata acgtaaacga aggcgcaaaa
    18241 aaagcggcca acagagagac ggcagataac gctgccattc gaatgatcaa ttttggacga
    18301 tccaccacgt cccctaaagg aactaaaaac agtaacccta acgcatagcc taattgggtc
    18361 aacatcgtca atatcccgac tgcacttgtg ctaacagata aactagctgc tacttttgtt
    18421 ccaatcggct ggatgtaata catattggca acaacaacgc cacaagtgat tgataatagt
    18481 aaaataagcg aagttgttaa aggttttttt tcattactct tcattgattt cccccatttc
    18541 ctatcttagt ttacaactta tggaaaagat tggaaataga aaaatacaac tattcgccac
    18601 acttattatt tgtgcttttg ttttccttgt tttttcttgc gatctttata aatgaagaca
    18661 actaacccta ctagcacagt aacacttgca accaacgtga ctgcagtttt gttatatccg
    18721 tgaaacagag cttctccacc acacgcatac aacacagacg aagggatgac tccaatcacg
    18781 atcgacaata tgatttgctg tagaggcaat ttcatcgcat ctgcagcaaa attgaccata
    18841 gaagatggaa taaccggtat catatagcct aacattacac ctatttttgg atgtttcatt
    18901 tgtcggattg cttgcaccca atgattcgtt tcggttttct tatctaaaaa ttttaagcga
    18961 tgcataagga aaattgcagt gatgttacca agagcattac ccaaaatgtt gatgatcgca
    19021 cctgtcagtg gtccaaagct caaaccagca atgactccaa ctactgaagt aggaatacct
    19081 ggaacagcac acatcaccgc tgtcaatacg atcaagagta tcgatgcaaa agtcccatgc
    19141 gaacgaatat gtgcgagtag ctctgcctta ctcgctctcg gatttaaaaa taattggatg
    19201 tctgtctgat attctaaata caaccgatag cccactacca gtatcagaat gattcccaac
    19261 gagatcaata tcaatcggat tattttcttt ttcatatttc ctcctgaaaa aaatctatat
    19321 tcattaaata atagtttaac ataaaaacaa aaaagcaccg aaatccaaca taggatctca
    19381 gtgcttttaa atagtaaaaa ttatttaaca gttacagatg cgccaacttc ttcaagttta
    19441 gctttcaatt cttcagcttc ttctttagat acgccttctt taactggtgc aggagcgcca
    19501 tcaactacag ctttagcttc tttcaagcct aaaccagttg cttcacgaac tgctttgata
    19561 actttaactt tttggtctcc agctgcagtt aattctacag tgaattctgt ttgttcttca
    19621 gcagcagcag cgccaccagc agcagctaca gctacaggag cagcagcaga tacgccaaat
    19681 tcttcttcaa tagctttaac taggtcgtta agttctagga tagttgcttc ttttaactca
    19741 gcaacgatgt tttcaatgtt caatgccatt ataatttcct ccgtttaatt gttctttttt
    19801 atttaatgct ttatgcggca attaagctgc atctccatct tctttgtctg ccactgcttt
    19861 gacagcgtaa gccacgttgc ggactggcgc ttgtagtaca gatagcaaca tagatagaag
    19921 tccttcgcga tttggcaatt ttgccaatgc tgtgatttct tccaatgaag agactttacc
    19981 ttcgatgata ccgcctttga tttctaatgc ttttgcttct ttagcaaatt cgtccatgat
    20041 tttcgctggt gcgactacat cttcgttact gaatgcaaca gctgtaggtc cagtgaatac
    20101 ttcgtccatg ccttcaaggc cagctttttt agctgcacgt gaaagaatag aatttttgat
    20161 aactttcatt tctacgttag catcacgaag ttgtttacgt aaacgtgtta cttcatcaac
    20221 tgttaatccg cggtagtcaa cgactactac tgatgcagct gctgtaaatt tttcgcttac
    20281 ttcatcaacg attgctgctt tttttgcgat aattgcttca ctcatttttg agtttcacct
    20341 cctgaatttt gatggaagag ttttttgcac tgaaaaggca agtcactttt atagttactt
    20401 tgctcaacca gtattattca tcacctattc acttcgttca tagtgattct actcgctttg
    20461 ctcgaaagga aactcctttc gctttgctca accagtattg ttcatcatct cttcacttcg
    20521 ttcatagtga ttcacaataa aaaactctat gccaccgcag acatagaggg actattgaat
    20581 atattcaata aacgtcctcg gtaggaaatt aaggcttgat gccacctact gtcttcggta
    20641 caagttaatt attaacctca actagcttag catgctaaac tagttgagtc aagaattatt
    20701 tttcagttgg atcaatcaac attctaatat ttaattagaa agaagcttgg tcaacatgga
    20761 taccaggtcc aaatgttgtt gtgatcgtta catttttcat gtattgacct ttagctgctg
    20821 atggtttagc ttttagcaat acatcatgga ttgctgcaaa gttttcaact aatttttcat
    20881 cgtcaaatga aacttttccg attggtacat ggatatttcc agctttgtcc acacggtaag
    20941 ttactttacc agctttcact tcgtttactg cttttgttac gtccattgta actgtaccag
    21001 ttttagggtt tggcattaag ccttttggtc ctaatactcg tcctagacga cctacagtag
    21061 ccatcatatc aggtgttgca acaactacat caaagtcaaa ccatccgttt tggatttttt
    21121 gaaccatgtc gtcgtcacct acgaaatctg caccagctgc ttcagcttct tttgctttgt
    21181 ctcctttagc aaaaactaaa acagtttgtg ttttaccagt tccgtttggt aatacaacag
    21241 caccacggat ttgttggtct gcttttttag ggtctacgtt caaacggtat gcaacttcaa
    21301 ctgttgcatc aaattttgca atgtttgttt cttttgctaa agctactgct tcggcaacag
    21361 gataaacttt acttgaatca acttttttta atgcatcttg catttttttg ctctttttag
    21421 ccattttggt tcctccttga ttgtggttat aacggttgta cctcccacgt gttctatatc
    21481 tttcaatata gagccaactg agaagctgct gtcttagggt gtatagatta ttctacagtg
    21541 atccccatgc ttcgtgcagt tccttcaacc atgcgcattg ctgcttcaac gttagctgcg
    21601 tttaggtctt ccatttttaa ttcagcgatt tctcttactt gatcgctaga aactttcgca
    21661 actttatttt tgtttggttc gcctgaacct ttttcgattt tagctgcttt tttcaataga
    21721 acagcagctg gcggtgtttt tgtaacgaat gtgaatgaac ggtcttcgta tacagaaata
    21781 acaaccggaa tgatcaaacc tgcttgatca gctgtacgag cgttgaattc ttttgtgaat
    21841 cccatgatat tgatacccgc ttgacctagt gcaggaccta ctggtggagc tggagttgct
    21901 ttacctgcag gaatttgcaa tttaacgatt ttttctactt tctttgccac gagacatacc
    21961 tccttaagtc cgtgatgtgg ttaattggag atgcatattt ctcctcccac gttttatctg
    22021 atctgaaaaa atccagacaa ataatgtgca aaacaatgca cactggtaca gtatagcaaa
    22081 tttatttgtt ttttcaagat ttttttctgc tatttttaca ttttgcttga ataaacgcta
    22141 aatttattct aaaaaaacaa caatttttcg ttctcatttc ttcttttatg ttacacttgc
    22201 gctagcatgg ttcaacagat gaaagcaaac aataacacgg ttttttagac ccttgtacaa
    22261 agattcctac ataacaaaat gaggtggaga aaatgaaaga tttaaggtat agaagtgtct
    22321 ttgatattat tggacctgtg atgattggtc caagtagctc tcacactgcc ggagctgcta
    22381 gaatcgggaa aatcatacgt agtatatttg gcgaacagcc tgattctgta gatatttatc
    22441 tatatgaatc atttgcaaaa acctatcggg ggcacggaac agatattgca ttagttggcg
    22501 gcctgttagg gatggaaccc gatgatgaac gtttagctga ttctttgaaa ttggcatatg
    22561 aagccggtat ggaagtcttg tttgttccta agagtgaaaa agccgaacat ccaaactctg
    22621 taaagatgct tctaaaaaaa ggaacacgaa agctttcagt cactggcatt tcgatcggcg
    22681 gtggcaatat ccagatttct gaattgaatg gattcaaaat ttctttaagc atgggtactc
    22741 ctaccttgat cgttgtccac caagatgttc caggtatgat tgctaaagtg actagtatac
    22801 tttctgagac caatacgaat atcggcacta tgacagtgac acgggaatca aaaggtgaaa
    22861 atgccattat gataattgaa gtagatgatc ctcaagtaga agaaacggta aaaagactca
    22921 aacagctgcc aaatatcgac agcgtaaatt atttcgatta aggagctaaa aaaatgtttt
    22981 tatcgattga agaattagta gatcaagcaa aagcttatcc aagtgtagca gaattgatga
    23041 ttgcagttga aatggaaatg agtggccgtt cccgtagtca catcatcgag atcatggaaa
    23101 aaaacctctc agtaatggaa caatcaatca acgagggaaa agaaggtgtt acctccgtca
    23161 caggaattac cggtggtgat gcaaaaaaat tagatgatta tttaaatgag gcagactttt
    23221 taagtggaga aacaatctta aaagctgtac gcaatgctgt agctgttaat gaagtcaatg
    23281 caaaaatggg gttgatttgt gctacaccga ctgccggaag cgcgggtgtt gttcctggtg
    23341 tgctgatggc tgtccgagat cggcttgatt tgacacatga acagcaattg gaattcttat
    23401 ttaccgctgg cgctttcggg ttagtcatcg caaacaacgc ttctatcagt ggtgcagaag
    23461 ggggatgtca agcagaagtt ggttctgcaa gtgcaatggc agcagcagct ttggtttgtg
    23521 caaaaaatgg gactcctcat caagcagcac aagcagtagc cattacattg aaaaatatga
    23581 tgggactgat ttgcgatccc gttgcaggct tagttgaagt cccgtgtgtc aaacgtaatg
    23641 ctttaggctc ttctcaagct tttatctctg ctgatatggc attagcgggt atcgaaagtg
    23701 tcattccgcc ggatgaagtc gttgctgcaa tgtaccaagt cggacgtcaa atgccgtcga
    23761 ttttcaaaga aacagcagaa ggtggcttag ctgttacgcc aactgctaaa cgacttgcgg
    23821 aagagatttt aaataagagg gcgtgataaa gctccttaat ttcgagtatt aagagattag
    23881 ttatcttcat aacaaaaaac gaagagagga ctaattgccc ttctcttcgt tttttattaa
    23941 tccatcaatt tcgtattttt tacttcatcg atcgtttttg ttcctgcaag ttgcatcgtg
    24001 atcgacaatt ctttgttcag gtgttcaaaa acagatgtta ctccttctgc cccacctaga
    24061 ttcaacccat aaatcactgg tcggccaatc gcaactaagt cagcaccact tgccagagct
    24121 ttgaagacat gctctcctcg acggacacca gagtcgaaaa tcactggtac acgtttgttg
    24181 actacttcgg caatttttgg caatacttca aaggacgcag gacctccatc tagttgacgt
    24241 ccaccatgat tggataccca gataccatct gcccctgcag agatagcgat tactgcatct
    24301 tctggcgatt gtattccttt tacgataact ggaagatggg tgatttcttt gattgttttg
    24361 atgtcatctg gtgtcaagcc ttgttttgca gctgcataaa tttctgcgat tccttttcct
    24421 tcgccattac cacttgcact ttgttcgcta tacgcagcta agttaggcat tggtaatgga
    24481 aattggaatt gattgatgac gtcttcttca cggtaaccac cgagcgttga atctgccgtt
    24541 aaaatgatag ctttagcccc tgcttctact gctttgttca aaatgaactc attgaatctg
    24601 tcgtctttac tcatatataa ttggaaaaac tgcggtgcgc ctggcgctgc atcggctgcg
    24661 tctttgattg tcgtatttgc ataagtgctg atggaaaaga ttgatccagc agcagctact
    24721 cctttagctg tatcagcttc ccctttttca tgtgccaatc cttgggccgc tgatggcgct
    24781 tggataatcg gtgtatctaa ttcaattccg aaaacagatg tatgcaaatc ggctgagtcg
    24841 attccgcgta atatccgtgg catgattttc tttgtattaa aagaagtcgt attttctttc
    24901 atcgtccact catcttcaga accaccgcga atatagccaa aagctcctgc ttccatgcgc
    24961 tcttttacac gaccttctag ttaggcaatg tttacaatat cgattggatg ttcttcatta
    25021 cttgtatgat aagtcatagt agatccttct ttcatcttct tgattacata taaatcatac
    25081 taaacttaca aaaagtaagc aaacatttac tctcagatac ttttatcgct attctttcca
    25141 aataaatcga cttacaagct gatctacttc ttggttttca tgaagcatac tatgagaggc
    25201 attttcacct gtcactactt tttcctctat ttcatttccg cgttgcttca acaacgcata
    25261 gaccgctaat gcactgttta acggtactgt tccatcactc aagtctgttt catctagttg
    25321 accagcaatc aagaaaaatc gagtggttat aggaatatta ccgatcattt gctggtaatc
    25381 ttgatagcgg ttagaaacga cagcaggccc ctttgctaac tcatctgtca gagactgcgc
    25441 actatcatcg acaaaatcat taaaaggcgc gccaattgct gttaatgtct gaacttgtgg
    25501 caatgacgta tcctgtccat acgttcctaa atagcgtagt gcactaactc ctcccatgga
    25561 atgccccact atgttgactt tatctatgcc gtagttttct ttcagataca aaagacaagt
    25621 tttgatccat tctgcttgat tccactcgtt atttttatta tcttcaaaca atacttggac
    25681 catcggattg tctgcttttc cagacaaatc tcctgttgct tgtatttgtc catctgttcc
    25741 tactgttaaa accaattctt ttactgcttg ctgatttttt tccaaacgcc gcaacatccc
    25801 tttaaaggaa ttttctgtcc cgctgtagcc gtggaaaaaa agtgttggaa tagtggtttt
    25861 ttctatcact ttactggatt ctgccgtttt ttctttatca gaaacttcag tagtatttat
    25921 cttcgtcgtc tggcaaccac ttaacgcaaa caaactgata aggactaaag ctgctgcagt
    25981 tgcacgtttg cataaatttt tgattggttc tgtcatacac ctcttccttt ccatatagaa
    26041 aaaagaaaga cttgaaaggc caatcatatg atcgacattt caagtctctt tattaatcga
    26101 ttttatctac ttgatcgaaa tcaagttctg tactggtttc acgtccaaac atatcgatgt
    26161 tgactttcaa tttttctttt tctccgtcaa cttctgttac ttgtccttct aatccagcaa
    26221 atgcgccttc gatgattttc acgacttcgc caacttctac ttctagatca ttttgtcgca
    26281 ggctcatacc gatagaacgc aagatatgat tgacttcttc tggaagtaat ggtgctggtt
    26341 tgcttccggc tccgtgtgaa ccaacgaatc ctgtcacacc aggtgtgttg cggacaacat
    26401 accatgaagc gtctgtcatg atcatttcaa caagtacgta tcctgggaat gttttatgaa
    26461 cgatttcttt ttctttccca tttttgactt cagtttcttt ttcttcagga acaacgacac
    26521 ggaaaataaa gtcggccatt ttcatacttt gcgcacgaga ttcgatattt gcttttactt
    26581 tgttttcata tcctgaatat gtatgcaaga cataccagtt tttttcaaaa gattccattg
    26641 aaggctcctc atttcattgt aaaataaaaa aacctcagta cacccgaagt ttttctttcg
    26701 cttcattata gcactaaaag tacgttgtgg ctagcgagtt attttaaaat ccagccaaat
    26761 aagctttgga tggctgtatc cattacgtaa aacatcaatc cgaaaataat agaagtttcg
    26821 atgactacta acgtatcttt acggagttgt tttctgcttg gccaagttac ttgtttgatt
    26881 tcatctttta cgctttttag aaatttcatg agacaacctc ccttgctact gtttatttcg
    26941 tttctttatg taatgtatat tgattacaat atttacaaaa cttattgatt tccaaacgtt
    27001 cgccgctttt tccttcactg actgattttg tgtagttgcg agacccacaa acggaacaag
    27061 cgagtgctgc ttttttcttt gccatttact tctgccccct tgctgagaat cagacttata
    27121 cttcataaca gtatcacgta actttttact tgtcaatttg tgaaataaac tatctattgt
    27181 tttggataaa attatgatat gattaatttg aaattaagct aaaatgagac aattggaggt
    27241 atagcgatgc tattaaaaac acttgtatat aagaaaaaag atctgacaac cgttacagag
    27301 actagtacat tagaagaagc attaaaaatt ttagaaactt ccggataccg ttgtgtgccg
    27361 atcttagatt cttcaggtaa tattttccgt ggaaacattt acaaaatgca tatctatcgt
    27421 cataaagcaa atggcggtga tatgacgctt cctgtcactc atcttttgaa aaacgctaca
    27481 aagttcattt acctggatac ttctttcttc aaagtattct ttacgatcaa agagcttcct
    27541 tatattgctg tccttgacag tgaaaatcat ttttatggta ttttgacaca tagtacgttg
    27601 cttaatatgc tctctcaatc ttggagcgta gaacaaggaa gctatgtact gacaattgct
    27661 tccacaggaa agcaaggaga tttggctgct attgcaaaaa tcatttcaaa acacagcagc
    27721 attgccagct gtattactct tgatatcgaa aaagacgaat acattcgacg tacattgatc
    27781 acgctaccgg ctaacactga agcgaaagta tgtgaaacga tcgtcaacca cttagagaaa
    27841 aagaattata aagtggtaga aattgaaaat ttgcaaaacc aagaagcata atcaaaaagc
    27901 gaaaaaagaa aacagctaat ctgctttctt ttttcgcttt ttataattag acacgctttt
    27961 caaaatcaag cgcgcgatct aaataataca tcaatggtgc tgaaacagcc atgacgataa
    28021 actcacttaa tgcaagcgtg ccataggttg tccaaaatgc ttggccacct gttggaacta
    28081 acatataagc aatcagtccc atgctgatgg taaagaagat cgtgttcaat actaaacgca
    28141 tctttacatt tttcactttg ttttgcaata aagctgtcag gcctaaagaa agcaatgttt
    28201 ggccgccacc aaaaaccaca tccatgatac caaaaccaaa gaaggcatta tagacgatga
    28261 ccccgcctaa aactccccaa agcaatttac ggttaaagac aactaaatga ttcaaacttt
    28321 ctgaaatacg aaactggatc ggccctgagg atacaggcgc aactaaaata gttaacgcta
    28381 gataaagagc catgatgatg ccatttacaa cgattacgga taaccgttgt ctgcttttcg
    28441 ttttttgcat tttctctcct cttttctcct agttttttta acgcgggatg gttgatgaac
    28501 cgcggttatg tgaaacaaac aataattata acagactttt catttatttc aatccgtctt
    28561 atctgctttt aaaaaagcaa aagcttaaac aatgtaaacg tttgctaaaa atcgtgggat
    28621 atggtataaa gtaagtgtaa ggggtcataa gaattcccag taaagcagac gaagtaggat
    28681 ctgcaagaac agaattgttg tgaaacttaa ataaataaaa aaggatgtgg gaatttgagc
    28741 aacctcttac tagagaacgg tctagcaagc agctgaagat ctaagtggac atgccgaacc
    28801 ttggaacttc tccttacgta agtaatggat catgcgcttg ctaggctttt ttgtgttttt
    28861 cggtcttcgg acctttttca ttttcctaga cttctgctat aataaccaag aattaatttt
    28921 gtaacagatt tttttgaaaa aacaagtttc tttcattcaa aaaatagaaa aagcttgcta
    28981 attattggtt aaccagcaaa caggagtatt aaaatatctt gttttattga atagcaggag
    29041 gaaatcatga aattccatta tcatatattg atcaatccat cggcaggcag cggaaatggc
    29101 tataaagtag cagaaagaat tttaccggta ttaaaaaata agcatattga ctatacactt
    29161 cattatagtg aatacaaagg acacgatgct gagattgctg aaactttggc aaaagaaaca
    29221 ttgattcctt ggatagaaga agaacagaaa acaatcgata ctttccccct tttgatcatc
    29281 gtaggaggtg acggcacctt acatcaggta cttgatactt tctatcagat ggaagtagaa
    29341 ttcccagttg cttatattcc agcaggttct ggagatgatt ttgctcgagg ggcaggcctt
    29401 ccaaaaaatc ccaaaaaagg acttcagctg attttagcag ctcaatcacc agaaaaagtc
    29461 cacataatgg cttatgaaga aaaaataagt gaaaaaaaag gaatagctgt taataatttc
    29521 ggtataggat tagatgcagc tatcgtccat gcgacaaatc attcttccac aaaaaagcga
    29581 ttgaacaaat acaacttagg atcattttcg tatttattct ctattctccg tgctttattc
    29641 actcaaaaag gatttccaat tctggtagat gcaggaggaa aacaattaag tttttcaaac
    29701 gcatttttat gtacagcaac gaagcatcct ttttttggcg gaggaatcgc gattgttcca
    29761 actgctgatg cgtctaaacc agtcattgat tttgtcgtgg tcgaacgaat caatttcttt
    29821 aaaatcttgt ggctcattct tttacttata cagaaaaaac aattgaaatc gaaatatttc
    29881 catcactaca caacaaacag attacgtatc gtttcaacaa caccgcaaca tggtcaagaa
    29941 gatggagaag aaatggaaaa acgacctttt gatatcacaa taaccacaac taaacagttg
    30001 ttttggtgtt aataaaagag gttttgtcag aagctacatc tggaacaccg tttattcgga
    30061 aaaaaggact gtgacacgac ttttgtcaca gtcctttttt tactatgcag ttaattcttt
    30121 gcccaattgg gttgccatcg atagtttctt tctttaccca aaataaattg gatcattgaa
    30181 aacaccgcag caaaagcaac taagacacaa ccattgaacc aaaaagattc tgcaaatgtt
    30241 tgattgtaac tgagtgtttc actgatcaag ttattatttt gaacaaacca atatgttggt
    30301 gtgaatgccg ctattttgct aaccatttct ggcaagtatt ctgctggtac aaacacccca
    30361 ccgataaagc aactgcctgt gataaaaata tctttgatcc caccgctggt ttctgagttt
    30421 tttaccaatg aagtgataca agccgaaaag ctaatgatcg gcaataaaaa caagaaagta
    30481 tttaaaatat gataccatac cgttatatcc cacgaggtgt ggatcactaa cagaaccatt
    30541 agaagtaaga agccactgaa caacagcagc gagtaactaa tcaaactaaa ggttactttg
    30601 cgattcaagt ttcgtttggt aatgggcgca ctctggtttc gtttactgat ctctttgcga
    30661 ttaaatgcta gattgatgac gctgaaaccg ctaaaaatgg atagaaacaa cccataagac
    30721 aagaaattaa aggttgtgcc cgtcaacagc tttttcatcc gctgactata tcccgcactt
    30781 agctcaactt ctccagtaac cgagagtgcc atttgtgtct gttccatagc tgcatcgatt
    30841 gttttatctt cttcttgaaa cagaatcagt gtttgcaaaa aggtgttcac ttgctgtgta
    30901 acaactgttt tagaaaaggc atctgggcga ctctgtatct tgatcgtcgg catctttcct
    30961 acttcgactt gttcggtaaa gtctgcaggc agatgtaaga taatgtttac acgattaaaa
    31021 taaagagcat catcgatttc tttttgtgag gttccatcca atgtaacgac tgtttccttg
    31081 tcattcaaat atttacgaag gccatcgact acaggcgact cttcttcact aatcaaggcg
    31141 attttcgcat cttgcagaga ggtattttca acatcaatat tttgcgcaaa aaagaatgtg
    31201 gcaacaacag tgataattac acctatcagt aaattcgatt tatttgcttt aagcaatgtc
    31261 caaatcgttt tataaagcgt cataacgcac ccctctttcc atccaagcat ttcctaacgc
    31321 aaaaaggaaa ataaatccga tcaaccatag aagattggta taaaaactag ccaaatcagt
    31381 gtagaaataa agttt
//
LOCUS       DACDTO010000024        30116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDTO010000024 DACDTO010000000
VERSION     DACDTO010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.024"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..604)
                     /locus_tag="IWK70_001157"
     CDS             complement(26..604)
                     /locus_tag="IWK70_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9613721.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(681..1211)
                     /locus_tag="IWK70_001158"
     CDS             complement(681..1211)
                     /locus_tag="IWK70_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9613722.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1215..2075)
                     /gene="rlmB"
                     /locus_tag="IWK70_001159"
     CDS             complement(1215..2075)
                     /gene="rlmB"
                     /locus_tag="IWK70_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9613723.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2068..2463)
                     /locus_tag="IWK70_001160"
     CDS             complement(2068..2463)
                     /locus_tag="IWK70_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9613724.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2460..3872)
                     /locus_tag="IWK70_001161"
     CDS             complement(2460..3872)
                     /locus_tag="IWK70_001161"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9613725.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(3869..4405)
                     /gene="cysE"
                     /locus_tag="IWK70_001162"
     CDS             complement(3869..4405)
                     /gene="cysE"
                     /locus_tag="IWK70_001162"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9613726.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(4727..6187)
                     /locus_tag="IWK70_001163"
     CDS             complement(4727..6187)
                     /locus_tag="IWK70_001163"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9613727.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6357..7514)
                     /locus_tag="IWK70_001164"
     CDS             complement(6357..7514)
                     /locus_tag="IWK70_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9613728.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7548..8918)
                     /gene="radA"
                     /locus_tag="IWK70_001165"
     CDS             complement(7548..8918)
                     /gene="radA"
                     /locus_tag="IWK70_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9613729.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(8981..9508)
                     /locus_tag="IWK70_001166"
     CDS             complement(8981..9508)
                     /locus_tag="IWK70_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9613730.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            9738..10544
                     /gene="proB"
                     /locus_tag="IWK70_001167"
     CDS             9738..10544
                     /gene="proB"
                     /locus_tag="IWK70_001167"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9613731.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10541..11788
                     /locus_tag="IWK70_001168"
     CDS             10541..11788
                     /locus_tag="IWK70_001168"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9613732.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            11864..12208
                     /locus_tag="IWK70_001169"
     CDS             11864..12208
                     /locus_tag="IWK70_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613733.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12325..12651)
                     /locus_tag="IWK70_001170"
     CDS             complement(12325..12651)
                     /locus_tag="IWK70_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613734.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13266..14468)
                     /locus_tag="IWK70_001171"
     CDS             complement(13266..14468)
                     /locus_tag="IWK70_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9613735.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14585..15100
                     /locus_tag="IWK70_001172"
     CDS             14585..15100
                     /locus_tag="IWK70_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9613736.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15299..15745)
                     /locus_tag="IWK70_001173"
     CDS             complement(15299..15745)
                     /locus_tag="IWK70_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9613737.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(15792..17270)
                     /gene="msr"
                     /locus_tag="IWK70_001174"
     CDS             complement(15792..17270)
                     /gene="msr"
                     /locus_tag="IWK70_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9613738.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(17624..18253)
                     /locus_tag="IWK70_001175"
     CDS             complement(17624..18253)
                     /locus_tag="IWK70_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9613739.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18237..18701)
                     /locus_tag="IWK70_001176"
     CDS             complement(18237..18701)
                     /locus_tag="IWK70_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9613740.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19188..20456)
                     /locus_tag="IWK70_001177"
     CDS             complement(19188..20456)
                     /locus_tag="IWK70_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9613741.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(20687..21424)
                     /locus_tag="IWK70_001178"
     CDS             complement(20687..21424)
                     /locus_tag="IWK70_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9613742.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21491..22060)
                     /locus_tag="IWK70_001179"
     CDS             complement(21491..22060)
                     /locus_tag="IWK70_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9613743.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22188..23060
                     /locus_tag="IWK70_001180"
     CDS             22188..23060
                     /locus_tag="IWK70_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9613744.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23402..24274
                     /locus_tag="IWK70_001181"
     CDS             23402..24274
                     /locus_tag="IWK70_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9613745.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24271..26229
                     /locus_tag="IWK70_001182"
     CDS             24271..26229
                     /locus_tag="IWK70_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9613746.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26561..27412)
                     /locus_tag="IWK70_001183"
     CDS             complement(26561..27412)
                     /locus_tag="IWK70_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9613747.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27535..28002
                     /locus_tag="IWK70_001184"
     CDS             27535..28002
                     /locus_tag="IWK70_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9613748.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28265..28747)
                     /locus_tag="IWK70_001185"
     CDS             complement(28265..28747)
                     /locus_tag="IWK70_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9613749.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(28847..29710)
                     /locus_tag="IWK70_001186"
     CDS             complement(28847..29710)
                     /locus_tag="IWK70_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9613750.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT         8593 a         6373 c         5455 g         9695 t
ORIGIN      
        1 agaagctcat taagattttt ctaaattatt cctcattata aaatttcaag tgttttttta
       61 ttttgagttt tactctcccg taagcgctca ttacttgctt gtgatccatt gctaaacatt
      121 cagccatgtc tacaaaatca acgccttgca tataataatg aaaaatttta gcttctattt
      181 ttgaaaactg gatttcgcaa ttttcgattg cttcttttaa taaaagatgc tgaattgcta
      241 aaggttcttg caatgtgatc tctgctaaaa agtctggtcc ttctgtctct attttttggt
      301 cgattgagac ttcatctttt tgatcacgac gcttcagcgc gttctgcttc ctcaacagag
      361 aatagatttg attgcttata acgcttttat aataacttcc aaagctaaca cctttgcttt
      421 tttcgtaagt tctcaatgcc tgatataaag cgatccttgc ttcttgttcc caatcctcag
      481 aaacataatt tcttaaataa taggtgtttt tcaaacgtag taaaagtgga aaatattttt
      541 gatacagggc agcaaatgct tcttcgtcac cattacgtgc agcgcgcaat aactcctcag
      601 acataaaata attcctccat aaattcgttt atggaggaat cttatcatca atgattcatc
      661 gaactgatta gaacgatatg ttatttattt tttctcatta aatcgtccaa tttttgggac
      721 aattttttta actgctcttc attccatgga gaattacgtc ggaaatcaat aaaatgcata
      781 tctttatttt cttgggctat tgttttttcc gtttttttta tcgtcttata taattcataa
      841 gcagaagttc gtaatgctcc ttgggaaaag atcatccact gttctgctaa atcgcttgtc
      901 gccactgtca cttgtgtcaa acggtcattc agctgaccag cgattctctc gatataactg
      961 tcagccgttt cgtcttcttt cgtaaaaaca acatccaact ggtatttctt ataattttgt
     1021 tggatgcccg gaactagttg ggcatcgaag acaacgatga cttccattcc ttcatatttc
     1081 gcataatttg ataaacgatg taaaagagct tctcttgcat ccgccatttt gtcctgcttt
     1141 ttaagctgga caagttccgg ccacgaacca atcatgttgt agccgtctac aatcaacagc
     1201 tgttttttca tggtctactc cccctgacgt tgggaaaaag cctgatacat aagcaaacca
     1261 gcagcaacac tggcatttaa gctctggacg tgcccggtca tcgggatcgt cagcaattca
     1321 tccatttctt ttttcaatcc tggactcatt cctttgcctt cattaccaat gactaaagca
     1381 attgatcctt tggcattcca tttttggaag tttgtgccgg tcatatctgt gccaaatacc
     1441 caaaatcctg tttctttgag ctgttgcaca gcttgtacca aattcgtcac gcgtgcaaca
     1501 gggacatgtt cgactgcccc agtggaagtc tttgttacga taggtgtgat tccaactgct
     1561 cggtgttttg gtatgatcac accatcgacg ccacttgcgt ctgctgtccg taaaatcgac
     1621 ccaaaattgt gtggatcttc gatgctatcc aaaatcaaaa agaaggggtc ttcttttttc
     1681 tcttttgttc gttcaatcag ttggtcaagg gataaatatt cataaggtgt aatcgcaaga
     1741 acgactcctt gatgcacgcc gtgatcgctc aaagtttcta atttttgttt aggaacccat
     1801 ttgactggca cagcttgttc acgtgcgatt tctttcagtt cttctacttt ttctcctcgt
     1861 gaatcttctt gaagaaagag tttattccct cgcccttgtt gtagcgcttc tactgcggca
     1921 tggtgcccaa agacgaaatt atcttctaat gcttcttcat tttgatcaga aactgactga
     1981 tttcgtccag tttgcttttt atttgaatat ctattttgtt ttttgtcttc aaatccacgt
     2041 ttcgattgat gtttgtttgt tctttcttta cccatttttt tcaccaactt tctgaataca
     2101 ccagcggatc aattcttcca tccgctcggt ttgttttgtc aaatgcagat agcccatgag
     2161 cgcttcgaac cctgttgcga cccgatacgt cgtaacatca gcattttttg cgctcgtatg
     2221 gcttttagca tttcttcctc tgcgataata aagttcttct tcttctgtca aaagatgttc
     2281 ttccagcatt tgctgaatca aaaaggcttg tgcattcgca gaaacataat tcgttgccgc
     2341 acgatgcagc ttattgggtt ttgtttctcc ttgatcaatc aggtggtcac gaacatagat
     2401 ttcgtaaatc gcatctccaa cataagccaa ggctaaaccg ttcaattgtg tgtaatctct
     2461 catgcttctc ttctccatcg ggttccttgc ggtgtatctt ctaataagat ccctttttct
     2521 ttcaacaaat cacgaatttc gtcgcttcgg acaaaatcac gattgcttct tgcttgattt
     2581 cgttcttcga tcaattgttc aatctcttca tctaataact cttctgctgt aaataaaata
     2641 ccgaagatcg aaagccattg cgagaataaa gcttctcctt gtgccaagac agcggcagaa
     2701 actttcggct ggtctagata agtgttcagc cacttagcaa gctcatagac cactgtgatc
     2761 ccatttgctg cattaaaatc atcgtccatt tcttcgatga atcgctgttc taaattttct
     2821 aacaaagcga ttttatccgc atcatctgcc aaattctctg aggcagtttc ttggcggaat
     2881 ttcaaattgt caaacgtatt cttcagtcgt tgtaaattgg tggctgcttc tttcatcgtc
     2941 gtttcactgt agcggatcgg acgacggtat tgtgtggtcg ccatgaagaa acgcaagact
     3001 tgtggatcaa cgtttttgat catttcatgt acagtgatga aattccctag agatttactc
     3061 attttttcat cgtcttcgcc aatcgttaca tagccattgt gcatccagta attagcgaat
     3121 tttttccctg ttttcgcttc actttgtgcg atttcatttt catgatgagg aaattctaaa
     3181 tcttgcccgc caccatggat atcgatcgtt tctcctaaat gttttgttgc cataacggaa
     3241 cattcaatat gccaaccggg acgtcctttg ccccatggtg aatcccaaga aatctcgcca
     3301 ggttttgctt gtttccataa agcgaaatct aatgggtctt ctttttgctg ctgttcttcc
     3361 cctgtacgct gacttgctcc gacttccagt tcatcgatgg attggtcact taatttccca
     3421 taattttcaa atttacgggt acggtaatac acatcacctg aagattcata agcatagcct
     3481 ttttcaacca acacttgaat aaatgctaaa atatctggca tatgatccat gactcttgga
     3541 tgcagtgtag caggttcgac attcaacgct ttcgtatctt cttcaaatgc tcggatgaaa
     3601 cgatcagcaa cttctggtgc tgtaatacct aattcgtttg ctgtacggat gattttatcg
     3661 tctacatccg tgaaattcga tacgaaatcg actttatagc ctcgatattc aaaatagcgt
     3721 cgaattgtat cgaaagcaat ggtgctacga gcatttccga tatggatata attatacaca
     3781 gtcggtccgc agacatacat accgacatgt cccttttgta tagggatgaa ttcttctttt
     3841 tttctggtca atgtattgta gatttttatc attctgatct cctcactttt tctcctttga
     3901 ttcgaacgac ttttgctggt actcctaccg ctgtagcgtc gtctggaaca tctttcaaca
     3961 caacggcacc tgcaccgatt ttggcgtttt ttccgatcgt taccggtcct aatatctgcg
     4021 cattcgcaga gagcatggct ccttgtttaa cagttgggtg tcttttaccc gtttcttttc
     4081 ctgtgccgcc taatgttacg ccatggaata aaatcacatc gtcctcgatt tctgcggttt
     4141 cgccaatgac gattcccatt ccatggtcga tgaacacgcc agttccaatc gttgctcctg
     4201 gatggatctc aacaccagtt aggaaacgcc agaattgagc attgatcttc gccaataaaa
     4261 agagccggtg ctgatataaa aaatgagaga aacgatgcca aaacaaggca tgaaaaccag
     4321 ggtatgtcaa aacgatctca gctgtcgaac gtgcagccgg atcgtttctc tttgctgcat
     4381 caatcgctcg ggtaaaccaa ctcatgactt tccactcctt tcgtaagaat tttatgtagg
     4441 aaatcatctt cttataaaga aaagaagcgc cttttaaata agtttcctta tcaaaagacg
     4501 ctttccgcgt ggttccactt ttcttcataa tcaatgatta tctctgacag ctaacgcctg
     4561 ttatacgttc ttggctactt tgtttcctat aaaacagttt ttcctgtttt ctgtgtttcg
     4621 ccaagaccac tcccagaggc atttcggcaa ccattcattg ataccttgca ccacccggta
     4681 tctctcttgt aatgatcagc tgcttacttt tttctgttca tcgtgtttag ttcagtactt
     4741 gatttaaatg tgctaatgat ttttcacgac caagcaattc gatcgtttca ggcaattctg
     4801 gaccatgcat ttgtcctgat acagctacac ggattggcat gaaaagattt tttcctttta
     4861 cgccagtttc tttttgaaca gctttgatgg cagctttgac agttggagca tctactactt
     4921 ccatttgttc tagctgtgct ttgaatgcgt ggagaacagt aggaactgtt tcaccagcaa
     4981 gtacttcttt tgctgcttca ttcaatacag gatgttcatt gaaaaatagc gtagacagat
     5041 caacgatttc tgcagcatag ctcatttgag gttgatacag gctaacgatc ttcttcaccc
     5101 attcgattgt cttttcatct gggttttctg ctacacgtcc atcagcgatt agataaggca
     5161 gacacatgtc agtaaatgca tctagatcca ttgctttgat gtaatggttg tttacccatt
     5221 gcagtttttt cgcatcgaaa gctgcgggtg atttacttag gcgttttgga tcgaacattt
     5281 tgatcaattc ttggtgttcg aagatttcgt cttcgcccac aggtgaccag cccaataatg
     5341 caataaagtt gaacattgca tctggtagat agcctaattc gcgatattgt tcgataaatt
     5401 gcaagattga ttcgtcacgt ttgcttagtt ttttacctgt ttcactgttg atgatcaatg
     5461 tcatatgtcc gaaggttggt ggtgtccagc caaatgcttc atagatcatc agttgttttg
     5521 gtgtattggc aatatggtca tctccacgta aaacgtgagt gattttcatc aaatgatcat
     5581 cgaccgcaac agcaaaatta taagttggca taccatcgcg tttttggatg acaaagtcgc
     5641 caccgatatt atcggattcg aacgaaatag cgcctttcac catatctgta aatgcgtatt
     5701 ctgtattgcg aggcacacgg aaacgtacaa cagattctag accttgtgct tctttttctg
     5761 cttgttcttc aggtgtcaag ttcgcacatg ttcctgcgta atgaggcatt tctcctcttg
     5821 cgcgttgtgc ttcccgttct gcttctaatt cttcttctgt gcaatagcat ttgtatgctc
     5881 gattgctagc aagcagctga tcaatcagcg gttgatagat ttctttacgt tcagattgac
     5941 ggtaagggcc gtattctcca ggattttcag gagattcatc ccaattcatt cccaaccaag
     6001 caaggttttc caactggctt ttttcgccat cttcgatatt acgtttttgg tcagtatcct
     6061 caatacgaat aataaagtct ccatcattgt ggcgggcaaa caaataattg aataatgcag
     6121 ttcttgcatt tccaatatgc aggtgcccag ttggacttgg tgcatagcgt acgcgtactt
     6181 tcgtcatttt cttcctcttc tttcaaacta gactttgact aacagcaaaa attccgtcag
     6241 ttacggcttc tcaaatatca tttatttaaa gccactcagc acaaatttta ccttttctaa
     6301 ggtaaatagt aaagccaact ggttgaattt ccttaccagt tggctgaatt tcttttttat
     6361 tttgattgtt tagtcttttt ctctgtctct tttttattgt cgattcctcg tcctgcatgt
     6421 gcaggtttag caaagatcat gcgtccagca gctgtttgaa gggcacttgt tacaacgact
     6481 tcgatacgtt cattcatgta atgctgtcca tcttctacga caaccatcgt gccgtcatca
     6541 aggtaggcta ctccttgttg gcgttctgtc ccggctttta ctactagaac attcatcgtt
     6601 tcaccaggga tgacgactgg ttttacggca tttgccaacg cattgatatt aagaacaggt
     6661 acattttgga attcagagac tttattcaag ttatagtcat tggttacaat gacaccatct
     6721 aaaagcttag ccaatttgat caatttgctg tctacttcac tgatatcttc aaagtctcca
     6781 tcgtacattt cgacagatat accctcttct ttttgcaagg catttaggat atctaatccg
     6841 cgacgaccac gcacacgttt caaactatcc cctgagtccg cgatgtattg tagttcgtaa
     6901 agaacaaaat tagggatcat caaggttcct tctaagaatc cagtctttgt aatatcataa
     6961 atccgaccgt caatgatcac gcttgtatcc aagattttat atttatggaa attttctcct
     7021 gattttcgtt ctaacagttg actctcaact tgactagctg tttcttgctc gatttttttg
     7081 cttcgtgaac caaaaacttt cttccattct tcgattcgtg tcgttcccat tctcaatccc
     7141 agatagccta agatgatcat cagtaaaatc ggcaatacac tattgacgaa tggaatttcc
     7201 atattgtaaa gaggggtcga gatcacaaca cctaaagtca atccgatgat cgcaccgatc
     7261 gcaccgaaga gaagataagt caagctgacc tcacttagtt tttgttctac ttgtttgata
     7321 atagctgaga tatgtttggc caacagcaaa gataataaaa agaaaataag tgcgcctaac
     7381 aggctgttgg taaatgtatt attcagccat ccgtttgact gttgaccgat tgcctgccaa
     7441 gctgagggta aaaatgaaat acccaagctt gcgccgacaa tgatcatcag caaagtaatg
     7501 acacgttttt gcatcgtttt tcctccgttt cgacaatcat ttattagtta gcggaacaca
     7561 cgcttcaacg tttcagccaa agttgctact ccaactatct cgattccttt tggcgcttcc
     7621 cagttaccta agttgttttt aggtaggtat acttttgtga agccgagttt ttggacttct
     7681 ttgactcgtt gttcaatatg actgacacgt ctgatttctc cagtcagtcc aatttcaccg
     7741 atgaaacatt ccgttggctg tgttcctttt tctttatagc ttgaagcaat actgacagcg
     7801 atagctaaat caatcgctgg ttcatttaat tttaccccac ctgcagcttt taagtaagca
     7861 tcttgattct gaagaagcaa cccagctctt ttttccagca ctgccatgat cagagatacc
     7921 cgattgaaat caagacctgt tgttgttcgt ttggcatttc cgaacatagt aggtgtgact
     7981 aatgcttgga tctctaccaa aattggacgt gtcccttcca ttgccacaac gatagcagag
     8041 ccagttgcac cgtctaggcg ttcttctaag aaaatctgtg aaggattagc gacttcttcc
     8101 aatccatgct catgcatttc aaaaatccca atctcattcg ttgagccaaa tcggtttttt
     8161 actgctcgca aaatccgaaa actatgatgt tgttcacctt caaaatataa caccgtatcc
     8221 accatatgtt ctaacattct tgggccggcg atcgagcctt ctttggtaac atgaccgacg
     8281 ataaaaatgg ctatcccatt tgttttagcg gttttgagta gttctgcagt ggtttctctt
     8341 acttggctga cacttcctgc cacacttgtg atgtctggct gcgtcatcgt ttggatggaa
     8401 tcaatgataa cataatcagg ggagatatgt tcgatggcac gactgatttc gttcatatct
     8461 gtttcagcgt atagataaaa ttctgtgttg atacttccga ggcgttccgc gcgtaatttt
     8521 atctgctctg cgctttcttc gccggataca tataagactt ttccgcctat ggctgctaat
     8581 tgctgagaaa cttgtaacag caaagtggat ttcccgatac ctggatcgcc accaatcaag
     8641 accatagacc ctggcaccac acctccgcct aacacacggt tcagttcttc taattttgtt
     8701 ttgatccgag gttctttttt agggacgacg tctgcgattt ttgttggttt tgctttttct
     8761 cctgtcaaac ttgtccgagt tctgcggtcc gttgtatctt gttcgatttc ttcgaccatc
     8821 gtattccatt ttccgcaatt tgggcagcgt cccaagaact taggcgacac atacccgcaa
     8881 ttttggcaaa caaattgtac ttttgatttt tttgccattg taccgcctcc ttttatccta
     8941 atcatgagac attaaatcaa gcttaagtat ctttaacgtt ttatttactg gaagaaccaa
     9001 atccgccttg acgttgagca gtcgtttgat cttgatctgc ttttaaaaac ggcaaaaaga
     9061 tcccttggcc gatacgctcg ccttttttga tcactacatc cttgaaacca aagttggtga
     9121 attggaacat gatatggcct tcatttgcct cattattgta ataatcactg tcaatcacac
     9181 ctactccgtt tgtcaacact aaaaaccgct tcaacggatt gcttgaacgg ttagcaagct
     9241 gcaaaaattc atcttctccc atgtaagctt tgattccagt aggcacgagg gttggtttca
     9301 attgtttttc attttctgat acatcttttt tcaatccatc tgcgactgtt tccaacccct
     9361 ttttccagcc tgctttccag atacttggaa tcacgacctc ttctgctgct tcaaaatcat
     9421 agccagcagc atgatgtgta gcgcgctgcg gtagattgat attttgatct ttatatttag
     9481 aaataacttc aaatcctctt tttctcaccg gttttcctcc ctaaataaac tactcctatt
     9541 ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa ttctgctatc
     9601 ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac aaaaaaacta
     9661 ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa tgagtggata
     9721 tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt attgtcaaag
     9781 taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca atcgatcagt
     9841 tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta gtctcatcag
     9901 gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact ttgattcctg
     9961 aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat aatcaaagat
    10021 tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg gtagattatc
    10081 ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg gacacgatcc
    10141 ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg acaaaatttg
    10201 gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac cttttgatcg
    10261 tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc gaggctcaga
    10321 tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt ggtgctggta
    10381 gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg atatttgaag
    10441 caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt gatattcttg
    10501 caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact tgatgcaatt
    10561 aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa accgcaaaaa
    10621 tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga tcattgaagc
    10681 aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga tgcaagaccg
    10741 cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa aaattgcttc
    10801 gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg gattgagaat
    10861 cggcaaaaag cgcgtgccac taggcgtgat cggcatcatt tacgaatctc gtccaaatgt
    10921 cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc ttcgcggtgg
    10981 caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag tactggtcag
    11041 tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg aaactgcagg
    11101 acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg gagcaaaatt
    11161 aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta caggaaataa
    11221 tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca tcgtcaatgc
    11281 aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc attctgagat
    11341 cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg tctctttacg
    11401 tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag acacagattg
    11461 ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt tggatgaagc
    11521 aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga cagatagtta
    11581 tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg tcaatgcctc
    11641 cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta tctctactca
    11701 aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa aatatatcat
    11761 ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc ccgccttttg
    11821 ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg aaattttaat
    11881 gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag tcggtatttt
    11941 atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa aatggtcgca
    12001 gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta gttacatggt
    12061 tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt caatcgctta
    12121 tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc agctgaataa
    12181 aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag agtgccttga
    12241 agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg tcacagcctc
    12301 tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt cctcttcgtt
    12361 aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct catccatgga
    12421 caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct gttcccgata
    12481 cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac cttccgcatt
    12541 gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg aaaattcaat
    12601 gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca tctattatcc
    12661 ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc agtttcatta
    12721 ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt ccgaataaat
    12781 ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca tgagaaactg
    12841 ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac ctcttggtaa
    12901 acggcgttca atcagctgac cctcggtatt agctcaaaaa acggtaaaat atgagaagct
    12961 atttttccgt tttgtttctc taatacttag gataaaacgc tgattttaca acctctgtta
    13021 aacggtgttc aatcagctgt ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac
    13081 tatttttccg tttgtttctc taatactcag gacaaaacgc tgattttaca acctcctgat
    13141 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgaga
    13201 agctattttt gtttggctgt tcttatgact tgatgcagga cagctttttc acaacctctg
    13261 atttcttatt cttcttcatt tgtagcaaaa taattacggt gataaatgat acaacataca
    13321 ctgataagaa caatggcacc tgcaaaataa aacgcatagc tttcaccaat atgttcacca
    13381 atcacacctg caataaatgg tgctaaagct ccaccgaaaa agcggacaaa actatagctg
    13441 gacgaagcaa cgtttcgttg cagacctggt atttccatag caatcgtcgt caacagtgta
    13501 ttgataattc cttggaaaaa tcctgctaac acaatgctga tcggtacaag gactggcata
    13561 gaagttccag ctcctaataa cagcaaacaa ataatgaata agacaaatgc tgctaaaatt
    13621 gtccgataag tcgtaaactt ccgctcaaga gccggtgcaa cgaaaatcga agcaatagcc
    13681 agaagcacac cccagccaaa aaatacaaaa ccgacttcta attcagaata gccaatcaat
    13741 aaaaatggcg catacgcaag taaagtaaag aatccgaaat tatataacaa tgcgattagt
    13801 ccaactgaac gaagtttatg attgcttaac gctttgacac cttcccaaag gcctactttt
    13861 ctctttggtt tctcagtttc aggtaaaagt accgctaggg ctataaaacc gcaaaacatc
    13921 aaaaatgcta ccccaaaaaa tggataacgc catgaaccac tgcccaagac cccaccgact
    13981 agagggccaa cagccattcc gcagccaagt gctgcttcat acatcatgat tgctttttct
    14041 gtgttaccaa ctaaaatact gacgatcgtt gctaaagcag tagagacaaa taaagcattc
    14101 ccgacacccc agccggcacg taacccaatc agcattccga tattcccaga taatccgcca
    14161 agaccagcga atacaacgat gataaacaat cctgtcatca atgttttttt caaaccgatc
    14221 cgactagata agaaacctgt aaatagcatc accacaccag tgactaacat gtaactagta
    14281 aataacaggg tcgtttcagc tggcgtagcg tttaactgtg cagcaatcgt ttttaagatt
    14341 gggtctacta gacccactcc cataaaagcg atcatacagg tagctgctac aactagcgct
    14401 gaacttttcc cctttgtttc ttcatttgtt tgttcttgta tatctatttg attctccaat
    14461 atttccatcc ctttctctct attagcatca tacatgattt attttacatg tcaatatgca
    14521 catgtaaaac gtggaaactt tactgtgata taataaagaa aataagacga gaggtaggat
    14581 ttttatgaat actttagcct atatattatt atgcgtgctt ttatccaaac cacgttctgg
    14641 gtatgaattg aagcagctga tcacaatttt ttgggaagcc catcacagtc agatctatac
    14701 aacgcttgcc aagttagacc agcagggtta tgtgaccgtt ttggacaaag acgagcactc
    14761 tcaaaaaaag atctaccact tgactgatga aggacgtgct cttgtagagg aatggatcaa
    14821 agaggaaact cccgctccta cgcaaaaaga tgaatttcta gcaaaaatct atgctttttc
    14881 gacgctcgat aaaacaacag ctactggact gctattcact agagaacaac agctgaacaa
    14941 aatcatgcaa aaaaatcaaa aaaaattgaa tgacctctcg ctagacaaga aacaagaatt
    15001 tggacgttat gttgtgatcc agcgcagaat tatgctttgt cagcaggaac tccaatggtg
    15061 tcagcaagta agagaacaaa tgcagcagtt ttttgagtaa gggataaaca gaacctttct
    15121 agaaatatct attgaagagc aaattatctt caataaaaga gcaatacagt tggaaaagaa
    15181 aaagcgatcc gaacgacagg ccaaaaccta tcgttcggat cactattttt ctagaactgc
    15241 attcaagaga ctatgacaaa aagcatgcca aggccttttt tcaaataaca gtgttccatc
    15301 attcattact ttgaattgct tttagcacac ttcttgaaag cgggccaacg atgatcaact
    15361 gcaacggtaa agcggcaatc acattcattt tccaagcatt catataagca gagaataatt
    15421 gatcaggtat tccttgttcc atgatcacac cgaataaaga catacatgtc accattccta
    15481 aaaccatcaa gcatgaaata gttaagatca tttggacctt gctttctgga tttactggta
    15541 gtttaaaggc aatttttttc gccagcacac cgatgatgaa gacatccaaa atgaaagcca
    15601 ccacgaatcc aggcactaga ccaatcaaca aacttgataa atgaagatct tgatgcagcc
    15661 ataaattata cgcgctcatc cccaagacca tcaaaaaaca catcattgtt gtaaaaatta
    15721 ttccttcttt tttatttgta ggcaatctgt accactcctt taaatttgtc acgaaaatca
    15781 tcgtagcaca tttaaaaatt tctcgtaagt acttttttta ttttctgccc ttttatttca
    15841 taaacttcat cagccgtacg ctcgacaaag taagaatcat gagaagtaaa caaaatcgtt
    15901 cccggatata tttgcattag cttctctaaa gcttcgatcg ttgccatatc aataaaatta
    15961 gtcggttcat ctaacagcaa cacattactt ggcttagtaa aaagcaaagc aatcgtcaga
    16021 cgggtcgctt ctcccccact taatgtacat aacggtttcg tcaaagtttc ctgagcaaac
    16081 cctaagtgat tcagcaaact acggacaagc gattctgaaa actctgtttg tctcatgaca
    16141 aaacgaatga tggattcttc agatgtcatt tgataaccct tttgctgata tacttgaaag
    16201 cttactttcg gagagagaag gattccttct ccttgtttgc ggatatgttc taataaggtt
    16261 gtcttacctg aaccattttc accgacaatc gcgatttttt tacctaaaga aaactgaaaa
    16321 tcaccattta ccagtaatgt tctcccactt ctttccaatt ggacattttg tcccatgatg
    16381 ggatagcggc tgtggagttc aagagatttt ggtactggaa aacggatttg tttcatctgc
    16441 tgcggcttgg ttgtttctcc tattttttgg agtcttctct ctaatgtttt cgcattcttt
    16501 tggatggctt tttgtacact gtctttttgt ttagaggaag acaaccgatc cagtctgatt
    16561 tgttgctttt tctttttact ggacactttt tccaactttt ccgcttgttt tttcttttct
    16621 tggatcgatt tttccaatcg ttttttctct ttcatgatcg actctgcttc tcgtagctga
    16681 gtctttttct ccaattcctt ttgagtgaga taggcactat aattccccga aaactctcgg
    16741 atttctccgt cttttacttc ccagattttc gatgctagct catctagaaa atagcgatca
    16801 tgactaacaa aaaccagcgt accataataa taacgaagct cttcaatcag tcgatcaata
    16861 ctttttctat ctagatgggt tgttggctcg tctagaagca tcccttgttc ataagaagaa
    16921 agaagacgta tcaactcgat ctttcgttct tcccctccgc tccatttccg ctctgaagtt
    16981 tcttccagtc ttaactggaa atgactggct aattccaaat ttaggtcctt tgcttcagcg
    17041 agataggtta attgaggcag gtagttgaag gtgatctctg tttggatttt cccatgatcg
    17101 ggaaaaagtt caccggctat tagtttgagc aaagtcgatt taccgacacc attttctcct
    17161 atgatcccta tacgatcttg ctgatagaca cgaagagaat caatagatag ttctagctgg
    17221 ttgccaaagc tgacttgtag atttgttata tttactgcta aattttccat atgttttaca
    17281 ctcctatttc atgtaaaaca acaattatcg atactaaaaa agagcagaaa aatctgtttg
    17341 caaaaaaact gcatcacatc ttttcctact ctgaacgaaa taactatgac taaatcacca
    17401 aatctgtcat agctttcacc tattctactg tcgttcgaaa aaatggacat actccatcca
    17461 tgaaattgtt gtttatacat gtcttttgaa tttatttagt tgttattcaa aagttcgaaa
    17521 cgtaatttca tcgatgcagt catttttccg acctcctttt ttattaggtt accacacacg
    17581 attcaaaaaa agcaatagct actgaatagt aactgaaaac cttttattcg aacatagtga
    17641 tcttttctgg tgctaaacga acagactcgt aaccttcttc taccgctttt cctattgata
    17701 aaataataac tggaacataa cggtctttat ccaaaccaaa tgcctctgcc aactgatctg
    17761 cttcgaatcc accaattgga tttgtttcat agccatgatc acgcgcaact aacatgaatt
    17821 gcatagccgc taagctagaa tcgactttga ctacatcgtt catttcttct cttgaaaaac
    17881 tcttgtaata aggaatgatc gcagctaatt gttgatctct gacttctttt ggcatcttcc
    17941 ctgattcgta agcttggttg tagatttctt cgccatattc gtaacattgc atatcgccaa
    18001 aaatcatgac cattgcagaa gaagtatcat tttgtctcgt attgaaacgg atcaaaggac
    18061 gcaagatttc ttttgcttcc tcgctttcag cgaccacaaa gcgccatggt ttcatattga
    18121 cagaagaagg tgcagtcgtc gcttctttga tcatttccag catttcttcg tgtgggattt
    18181 tgtatgtttc atcatatacg cgaacggatc ttctaccagt cataattgtt gaaaaatcat
    18241 ttttttgttc cattattatt aacctctttc gtttctgttg ttatattgca aagttgggta
    18301 ttcaatttat ctaaaagtgt tagaaattgt ttcatttcta catcgctgaa agccggggat
    18361 agcacggaac ctgtatcttt atggtccatt tcacaatgct tgattttctt ctgggctttt
    18421 tctgttagcg ctacatacac ttcccggttg ttctgaggat ttctctgacg cgaaacatag
    18481 ccatttgctt ccaatacctt cagatgacga gtaactgctc cttgatcaat ctgcaacttt
    18541 tcttgcaaac tgctttgtag caatggattt tcttctttca gtctcatcat gatctcgtat
    18601 ctagtaagac ttataccgat attttcttca aagatctgga cgattttttg atcggctaat
    18661 ttgatttgat ataaaaggtg actaatctct tctaaagaca taatttcctc ctttctaata
    18721 attgactcgt caacgattga cttgtcaata atagaataaa aaaagaaaaa agtcaaagaa
    18781 tcagctgtgt aataaaaaaa acgctctcaa tagaaaagta tagagaaaag aaattttttg
    18841 tagaatgaac ggaaaaaatg ataaagaaag cacaacgcta aaaccaagat tattgattct
    18901 tatcaaacta tagaagatag ctcttctgca gtagataatt ttacaccgcc aaaaatttcc
    18961 gaaatcgcct tttgcaagaa tcccgtcgaa tattagaaat tggatcagta tgcttaaaca
    19021 gtaataaaaa agttatgaca agatgttgtc acaacctttt ttgagtaaac ggtgttcaaa
    19081 aacttgcatc tgcggtaata agttcaacca aacaaaaaac atgaaaatct gtttttgttt
    19141 ggctgttctt actacttgat gcagggcagc tttttcacta cctcttttta ataaccataa
    19201 aatcctattc cttcatatct ccagcccatc ggtgttagcc aatctctttc cttagcactc
    19261 aaggtatagt gatgggtacc agttttttca tttggattaa atagtcgata aattggcttg
    19321 gaaccaccag aataaaaact tagaccttca taacgccaac cgatttttgt taaagagttg
    19381 atctcagatt gtgccattgt gtaatgatgg tctccactgt aaggattgta taaacggtag
    19441 acaggttgtc cgctgtttgg tgcttgccaa gcaatccctt catattccca gcctacagtt
    19501 ggcagataat ttttttctcc ggcattcaat gtatataaat gttctcctgt atgacggtta
    19561 tacagacgat acatcggaga agttgaagtt ggtgctgtat catactgtgt cagattatag
    19621 gtttcgatta cattgttcaa tgtagaacca taattaggcg cagtggcata acgacctgtt
    19681 agccaagccg tagcatcttt gtaagagttc gtattgcttt tccatgcacc ggaatagtag
    19741 taaacgcctg gctgaaaaga agtcgtcttc aataccgtta cgttatcttg caatgattct
    19801 ttgtacgaag gatattttct aaaagcggca ttcaccgtaa tccattgccc attcacatat
    19861 tccgaagtag gcatcgtaac agattggcca ttatagcttc ctttaatccc aaatagattg
    19921 taattagggg cagaagccaa agtactgttc ccccagccac tttcaacgat ggcttgagca
    19981 atcatgactg atgcatataa gtcattggca cttgccaaac tttgtgcagt tgaggcaata
    20041 ctatcaatga acgcttgttg agaattagca gatgagcgag cataaaaagc atagccagta
    20101 tagttttcga attcagacaa atctgcttga tgggtatgtg tcaatgtttg cccatctgtc
    20161 aattggttcg tgatttcttc tgcattctct ggattgttct gagcttctgc tgatgtactg
    20221 tcttcggttt gtgtttgttc tgttgtttca gttgttggtt cttcggttga aattgtattt
    20281 tctgagacag tagctgactg caaagtactt tctgtatcgt tttcaattcc tgtttctgaa
    20341 gaactcgatg tctgagttgc gacagtttct gtcgattctt cagctagcac tgttgtgttg
    20401 gcaactgcta gatttcctaa aagaataacg ctaaataata aattctttgc tttcatcttt
    20461 tttctccttt gaattatgaa acttttccta aataacagtg taactaaaaa agtaaaagca
    20521 agtcaatgtc catatttaaa taacatttgt ttctttttaa taaatttgct ttttctgcat
    20581 aaaaagacgt aaatgaagaa tattcttctc cattcacgcc ttaaattgat ggtcgtttga
    20641 ataactattt aacaagtaat tctctctgga tattatccgt tatggattat acttcctgtg
    20701 tcgtcggacg gataatcatg ttgctgacag acatccggtc tggcgtttct atcgcaaatg
    20761 ctacagcaga agctatatct tcagatgtta atccccattc tttttgcgct tcatgaagtt
    20821 caagagcagc ctttttgtcg ctgatggttt gatataattc agtttgaaca gctcctggag
    20881 agatgatcgt agatttgatg ttattttgcc gttgctcttg acgtaatcct tccatgattg
    20941 cacggacagc aaattttgtt ccgcaataaa cggcggaatc aggataaaca acatgtcccg
    21001 ccacagaatc agttgaaatg atatgcccag atttttgttt ttccatgatt ggaagtacgg
    21061 cggaaattcc atttaacaca cccatgatgt tgatatcaag catattttgc cattcatcac
    21121 ggtgaccttc aactaatgga gcggttggca tgattcctgc attattgtac aatacatcga
    21181 ttcgaccgaa cttttccatc gctaaatcga taactgcttg gacttgtgca aaatctctta
    21241 catctgctgt ttgaatatat agctcagctt ctggtaaaga ttcttttatt gcttttaggc
    21301 gatcttctcg gcgtgcagct ataactagtt tagccccttt ttctgctaat agcttcgttg
    21361 ttgcttcacc gattccgctt gaagcgccca tgatgacaat cactttttct gataaatcat
    21421 tcattctatt ctcctcctgt tttgaaaaat ctcttgctta tactcactat ctcttatttc
    21481 atcttactgt tcaaataatc cgttcgacag gtttgacgat tttggcaggg attcctgcaa
    21541 cgatggtatt gtctgggaca tctttcgtaa cagttgcatt cgctgcaaca atggcattct
    21601 cgccaatcgt tacgcctggc agaatagttg ctcctgctcc gatccaagca tttttcttga
    21661 tgtggattgg ctttactgac aagcctcttc gttcttttgg atcggttaga tggttcaccg
    21721 tgatgaggtt gacacgagga ccaatcaata ctgaatcttc taaacagatg ccacctaagt
    21781 caacgaacat cacttgacga ttgataaaga catttttccc tatactaata tgtttgccga
    21841 aatctgtata aaaaggcagc agaacagtca ccgtatcatc tactttgtca ctaatgatat
    21901 tttctagccg tttgcggata tctgctggtt catggtaccc agtattcagc gctgctaaag
    21961 gctcttcgtt ttccgcttgg atctgatgga tcacttcaaa gagcggatcc gttactcgaa
    22021 tctctttatt gacgatttgt ttttcgatga ggcttgtcat tttgctccct ccttttatcc
    22081 ttactttata acggacggct tatgatgtct aatacttata aaacatactt aactattcct
    22141 tttaactata cttagtggta tgctttttta aaaacaggag ggaaaaaatg gatattcgta
    22201 tactgacgta ttttttagca gtagctagag aaaaaaatat cagccgagca gcaaaatctc
    22261 tgcatatcac gcaacctact ctctcaaaac aactgaaaga tttagaagaa gaacttggtg
    22321 tgacactttt cactcgaggc tcccgagaga tacagttgac ggattctggt cgatatcttt
    22381 attcaaaagg aaaagaaatc ttgtcgttgg tagaaaaaac ggcgaacaat ttaacaaatg
    22441 acgcaatcat ctctggagaa atctacattg gcggaggcga aactaaagca atctctttca
    22501 tttctgatag ccttcattcg ttgatcaagg cgcatcctga tatccagttt catttatata
    22561 gcgggaatgc agatgatatc atagacaaac tggatagcgg attacttgat tttggactcg
    22621 taatcgaacc gacaaacaaa caaaaatacc agtcatttcg actgccagcc caagatcgtt
    22681 ggggactttt gatacatcgg aatcatcctt tagcaaagca aacagtcatc catccgcagg
    22741 aactgaaaac tgttcctttg atcatttccc ggcaaacgtc agtggacagt caactagctg
    22801 aatggctagg tttcagcata gatcagttat cgatcgtcgg aacatataac ttattgtaca
    22861 atgcttcttt gatggtcgat aacgggactt ttggtgcatt agctctagat gggatcatca
    22921 atacttatgg gacggaaaaa gtattcatcc cactcgatcc gccattacaa gcttcactga
    22981 atctcatttg gaaaaaagac cagcctcttt ctcctgctgc tcgtaaattt ttagatgtcc
    23041 taacggagaa atacagttaa gtagagtttt tttccttcgc tttcagcttt ttaacaacga
    23101 ataagttaag caaaagatcc aatgattaaa taacataagt ttcttaattt gttccttcct
    23161 tttatttctg ataattccag taaattacta taattataaa aaaagaattg acaaaacata
    23221 actgtctata taaaattgat tacgttaaca ataattcgtt tcatgtgact agatcatact
    23281 aggcatgaaa aatctctgag gaacatggtt tccctaggag gtttttcgtg tctatttttg
    23341 tttggccgca atcaactggt ctagcaaacg tgaaaaccaa atcttcaaaa agggtgcgcg
    23401 catgaaaatc aaaaagatct ttaatcaaaa tgcagtattg gtagatgacg gtggacagga
    23461 aaaagtagct attggaaaag ggattggatt tgacagaaaa agaaatgatt tgatttttga
    23521 tcgtgatgtc gaacggatat ttgtcatgga accagaaggt caaatcaagt tgcaaaattt
    23581 acttaatcag attgatgaac gctttttatt tgcagcagaa acgatcattg atcatgcgga
    23641 aactgttttg atggaaaagt tgaatgaaca tgtattgatc gctttgaccg accacattgc
    23701 tttttctgcg gagaacatta ataacggtat cgtcatccgc aacaaacttt taagagaaat
    23761 cgaagtgctt tacagtgatg aattcagtat tgctcaatgg gcagtagagt atctgacaaa
    23821 agagcttggg attccatata cttatgacga agcaggatat atcgcaatcc atattcacag
    23881 cggacgaagc ggtcggaaca ataaccatcg cagtatccgt gaagtgacga ttgtatctga
    23941 catcatccat ttagtggaat cagaattgga aacagatatc cattcagaac cattttcact
    24001 gaattattca agactggtga atcatttacg actgcttcta caacgaaccc atgcgcaaca
    24061 atatgctgtt ttagacacgg aaatcgtaga aatggtcaaa cgaaaatacc cagaaagcta
    24121 taagatttct aaaaaaatcc gtgtgttgct gaccaaagaa taccagctcg cgatcacgaa
    24181 agaagaactg ggttacttgg ccatccatat cgagcgttta agaagcgcaa tcgtacaaac
    24241 aaatgttaac aatcatgagg aggaaaaaaa ttgaaagcat atctacaacg aatgggacgt
    24301 tcccttcaac ttccagttgc ggtcttacct gctgctgctt tactagtagg gatcggaaac
    24361 tggtgggcat cttatagtaa cgacattgtc gctcattttt tacaagctgg cggtaacgca
    24421 gttttaggtc aattaccttt gctgtttgcc gttgggttag cactagggat gtcagtggac
    24481 aaatcaggtg ctgctgcctt ggcaggtttg gtcgcatttg aagttccagt aaatgtttta
    24541 aaaactgatt cagtagctac tttattgaat atcaaaccag aatcagttga tccatctttt
    24601 gctcagatca gtaacgtatt tattggtatt attgccggat tgattgcggc agctctttat
    24661 aatcgtttcc acgaagcaaa attaccgatg gctttatctt tctttagcgg caagcgtcta
    24721 gtgccaatct tggctgcctt tgtcatgttg ctgatcagtg cagtgctact gctcgtatgg
    24781 cctccggttt actctgcttt agtttctttt gctaaattca ttttgagttt aggctggggt
    24841 ggtgcaggtc tttatggctt cttcaaccgt ttactgatcc ctacaggatt gcatcaagca
    24901 atgaactccg tgttctggtt tgacgtagca ggaatcaatg atattggtaa atttttggct
    24961 tctgaaggaa caaaaggcgt aacaggaatg tatcaagctg gatttttccc aatcatgatg
    25021 ttcgggcttc cagcaggagc atatgctatt tacagaaatg cacgtccaga aaacaaagca
    25081 aaaacagctt ccttgatgtt agcagcagct tttgcttcat tctttactgg tgtaacagaa
    25141 ccacttgaat tctcatttat gttcgttgct tggccattat atgtcttgca tgcaattttc
    25201 actgggattt ctttagcagt gagtgcattc ttccattgga cagcaggatt tgcctttagt
    25261 gccggatttg tcgattactt cctatctttg aaaaatccga ttgcgaatca gccattgatg
    25321 ttgatcgtcc aaggattagt aacagcagtg atttactact ttggctttaa ctttgctatc
    25381 aaaaaattcc atctgatgac tcctggccgc gaggaagcag atctttcaga tgacgatact
    25441 gcaacgacta attcagacaa taaatatgcg gcacaagcaa gcaaaatcta cgctgcatta
    25501 ggcggagccg acaatgtaac ttcgatcgat aactgtacta cacgccttcg tttgcaagtc
    25561 aaagatacag gcctgatcga tcaaaataaa atcaaagcca ctggcgtacc tggtatgaag
    25621 atcatcgacg gaaaaaatgc gcaagtcatc gtaggtacag aagtacaatt cgttgcagat
    25681 gagatggcaa aactacatgg tggtgctgca acacgacctg cacaaacaaa caccgtggtt
    25741 aaaacagaaa catttgccgg tggaacgatc gaacaagata tttacgctat cgcaaatggg
    25801 aaactgattc caatcactga agtttcagat gatgtattcg cggaaaaaat gatgggtgac
    25861 ggctatgctg tattaccaga aaatggagaa atcttctctc caatcgctgg tacgatcaca
    25921 aacattttcc caacgaaaca tgctgtcgga atccaaacag atgctggtat cgaagtcttg
    25981 cttcatatgg gcatcaacac tgttgactta aaaggcgaac cattcacttt atatgtaaaa
    26041 gaaggtcaaa aagtcgcaag aggtcaattg attgctttag ttgatcttgc cgctattcaa
    26101 tctgcaggca aaaacacgga tatggtcgtt gtattcacaa atggcgacaa agtccaaagc
    26161 ttagaaatcg aacccgcacg tgatgtaaaa gcaaatgata aaatcggctc tgtttccaac
    26221 aaagcctgag ccatcgtctt ccccagactt ttttatcgat aaacaaacag gagctgagac
    26281 aaaaatcttg tctcagctcc tttttccgaa taaaaacggc gttctcctgc caactccgcg
    26341 gtaataagtc cagccaaacg taacatatga agagctattt tttatttgac tcttcttatt
    26401 acttgatgca gaacagcttt tttacaacct ctgataaacg gtgttcaaaa accagtttat
    26461 cggtatgaag tcaaatttat gcaaaaatgt ggaaagccat tttcacaaaa tttttcttca
    26521 tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga tccaatattt
    26581 cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct aaatcatcag
    26641 aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac atgtcagctg
    26701 cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca cttgcttcaa
    26761 taatttctaa ctttttacct gtcgcacgct ctaatgcctt ggctaattct tcatagtcag
    26821 ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt acttttgctg
    26881 cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct gcagcataaa
    26941 cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct acataccaat
    27001 tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca gtgaattggt
    27061 gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta taagcgatat
    27121 aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct tgacgatttc
    27181 ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct ttcattgagt
    27241 ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt gcttctcctt
    27301 tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt tttaagaatt
    27361 ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc atatttgcca
    27421 ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt acacctttat
    27481 cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca actgatgaag
    27541 tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat gtttcaagat
    27601 acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc agcaaatgta
    27661 cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac cggaaaaccg
    27721 cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta tcgcagcatc
    27781 gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg cgtcgttggt
    27841 gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa ggcagaagaa
    27901 gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt ggaaaaagaa
    27961 aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct actgtgcctg
    28021 catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga caaaagcctt
    28081 gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat gacgtcttga
    28141 taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa aatatggaaa
    28201 gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca cgacctctat
    28261 attatcatgg tttattgact accggtatcc agatttctcc catatatttc ccgttggtaa
    28321 ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct acttctctta
    28381 aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc gattctgcta
    28441 aagcgattac aacaagatac gttccttttg ggaatgggat gattcgggta gtgtttggaa
    28501 catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag ggttcgttga
    28561 ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt ccattaccga
    28621 taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc gtattgctac
    28681 tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg atcctatact
    28741 ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa ttaaagggaa
    28801 atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat ttttttgctt
    28861 tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat tttgtaaaag
    28921 agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct gcatagattt
    28981 tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa atggcccaag
    29041 tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt gtcgtagcaa
    29101 agccaatcat gtgcatcaaa ctgccctttt cttccaagcg ggctccatca aatccataag
    29161 aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct tccctttgag
    29221 tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca aatttgacag
    29281 gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata cgaaattcca
    29341 tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat ttttgtatgc
    29401 cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct cttgaaaaac
    29461 cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat ccatctagga
    29521 gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc gacatccctg
    29581 caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt tcggctatta
    29641 ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat tggttcagat
    29701 ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca aagaaagtca
    29761 attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta tggatctaaa
    29821 gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta tctatgtttt
    29881 acctttagaa aaataaattt tggggaagac acattctaag taaacaaagc ggtaaaaata
    29941 tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat tgttgttcaa
    30001 actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct cttttatcaa
    30061 ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa ataaaa
//
LOCUS       DACDTO010000025        27975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDTO010000025 DACDTO010000000
VERSION     DACDTO010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.025"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWK70_001187"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWK70_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9613751.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWK70_001188"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWK70_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9613752.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWK70_001189"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWK70_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9613753.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWK70_001190"
     CDS             1642..2274
                     /locus_tag="IWK70_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9613754.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWK70_001191"
     CDS             complement(2276..3181)
                     /locus_tag="IWK70_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9613755.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWK70_001192"
     CDS             complement(3266..3919)
                     /locus_tag="IWK70_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9613756.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWK70_001193"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWK70_001193"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9613757.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWK70_001194"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWK70_001194"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9613758.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWK70_001195"
     CDS             6168..7253
                     /locus_tag="IWK70_001195"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9613759.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWK70_001196"
     CDS             7250..8314
                     /locus_tag="IWK70_001196"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9613760.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWK70_001197"
     CDS             complement(8563..9366)
                     /locus_tag="IWK70_001197"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9613761.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWK70_001198"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWK70_001198"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9613762.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWK70_001199"
     CDS             complement(10244..11251)
                     /locus_tag="IWK70_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9613763.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWK70_001200"
     CDS             complement(11258..11902)
                     /locus_tag="IWK70_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9613764.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWK70_001201"
     CDS             complement(12002..12760)
                     /locus_tag="IWK70_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9613765.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWK70_001202"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWK70_001202"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9613766.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWK70_001203"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="IWK70_001203"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9613767.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWK70_001204"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="IWK70_001204"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9613768.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWK70_001205"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="IWK70_001205"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9613769.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWK70_001206"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="IWK70_001206"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9613770.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="IWK70_001207"
     CDS             18158..19027
                     /locus_tag="IWK70_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9613771.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="IWK70_001208"
     CDS             complement(19153..20052)
                     /locus_tag="IWK70_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9613772.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="IWK70_001209"
     CDS             complement(20217..21041)
                     /locus_tag="IWK70_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9613773.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="IWK70_001210"
     CDS             complement(21052..22347)
                     /locus_tag="IWK70_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9613774.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="IWK70_001211"
     CDS             complement(22363..22842)
                     /locus_tag="IWK70_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9613775.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="IWK70_001212"
     CDS             complement(22848..23876)
                     /locus_tag="IWK70_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9613776.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="IWK70_001213"
     CDS             complement(23893..24999)
                     /locus_tag="IWK70_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9613777.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWK70_001214"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="IWK70_001214"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9613778.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7889 a         6133 c         5046 g         8907 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg ctttatttac
    27961 aggtgtttgt ataga
//
LOCUS       DACDTO010000026        27934 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDTO010000026 DACDTO010000000
VERSION     DACDTO010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27934
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.026"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(409..1353)
                     /locus_tag="IWK70_001215"
     CDS             complement(409..1353)
                     /locus_tag="IWK70_001215"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9613779.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1649..2593)
                     /locus_tag="IWK70_001216"
     CDS             complement(1649..2593)
                     /locus_tag="IWK70_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9613780.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2814..4028
                     /locus_tag="IWK70_001217"
     CDS             2814..4028
                     /locus_tag="IWK70_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9613781.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4050..6065
                     /locus_tag="IWK70_001218"
     CDS             4050..6065
                     /locus_tag="IWK70_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9613782.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6148..7521)
                     /locus_tag="IWK70_001219"
     CDS             complement(6148..7521)
                     /locus_tag="IWK70_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9613783.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7547..7831)
                     /locus_tag="IWK70_001220"
     CDS             complement(7547..7831)
                     /locus_tag="IWK70_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9613784.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7833..9848)
                     /locus_tag="IWK70_001221"
     CDS             complement(7833..9848)
                     /locus_tag="IWK70_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9613785.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10027..10332)
                     /locus_tag="IWK70_001222"
     CDS             complement(10027..10332)
                     /locus_tag="IWK70_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9613786.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10421..11452)
                     /locus_tag="IWK70_001223"
     CDS             complement(10421..11452)
                     /locus_tag="IWK70_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9613787.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11659..12534
                     /locus_tag="IWK70_001224"
     CDS             11659..12534
                     /locus_tag="IWK70_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9613788.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12686..13528)
                     /locus_tag="IWK70_001225"
     CDS             complement(12686..13528)
                     /locus_tag="IWK70_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9613789.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13620..15497)
                     /locus_tag="IWK70_001226"
     CDS             complement(13620..15497)
                     /locus_tag="IWK70_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9613790.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15494..16915)
                     /locus_tag="IWK70_001227"
     CDS             complement(15494..16915)
                     /locus_tag="IWK70_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9613791.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16918..20307)
                     /locus_tag="IWK70_001228"
     CDS             complement(16918..20307)
                     /locus_tag="IWK70_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9613792.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20564..21955
                     /locus_tag="IWK70_001229"
     CDS             20564..21955
                     /locus_tag="IWK70_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9613793.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22028..22675)
                     /locus_tag="IWK70_001230"
     CDS             complement(22028..22675)
                     /locus_tag="IWK70_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9613794.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22683..23369)
                     /locus_tag="IWK70_001231"
     CDS             complement(22683..23369)
                     /locus_tag="IWK70_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613795.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23375..24448)
                     /locus_tag="IWK70_001232"
     CDS             complement(23375..24448)
                     /locus_tag="IWK70_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9613796.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24670..25344
                     /locus_tag="IWK70_001233"
     CDS             24670..25344
                     /locus_tag="IWK70_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9613797.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25349..26689
                     /locus_tag="IWK70_001234"
     CDS             25349..26689
                     /locus_tag="IWK70_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9613798.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26838..27824)
                     /locus_tag="IWK70_001235"
     CDS             complement(26838..27824)
                     /locus_tag="IWK70_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9613799.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8148 a         5270 c         5060 g         9456 t
ORIGIN      
        1 ctctcgaacg gttttttatc aaaaatcgtc atttttccat caaaatctgc ttatgcaaaa
       61 ttataaaaaa attttgcagt ttttcttact ttttttgtcg tttaattgaa tgataaaaac
      121 cagtttcttt attcagttaa tcaaatatga acgaattaga tatgaatcat tgtatttgcc
      181 gttgcggaag gattttttaa ttaaccacag ataaacattg ataacgattc gcttattgta
      241 atcaacagat taaatttcat atgttcatat gtacggatac attttcggac cgattaattt
      301 taaccattct gtttatttgc ttacctatta gatatcaata aaaaactgct taacagctat
      361 ttcagctgtt aagcagtttt aggattttta gttcatattc attgtttctt ataatacagt
      421 gttcaatact tctcttaaag cacttgttga tttgctgaac attgcttttt cttcttcgtt
      481 gattgataat tcaacaactt cacgaacacc attttcgtca acgattgatg gcacacctgt
      541 aaagatatct ttttcgtcgt attcaccagt taaataagca gatacaggca atacagcttg
      601 ttcgttattc aagatagctt ttacgatacg tgcagtactc ataccaatac catagtaagt
      661 tgctttttta cgatcaatga tctcataagc agcattacgg actttatcag caatcacatc
      721 taattcatct tgggcaatac gatggtcttt ttctacaatt tcaaacactg gtttaccacc
      781 gacagttgta tgtgaccatg cagcaacttc tgaatcacca tgttcaccta agatatagcc
      841 gtggacgctg cgtgggtcaa cttttaattt caatgcgatt tctttacgga aacgagttgt
      901 atccaatgtt gttccagtac cgataacacg tgatgttggc agaccagatt cgttccaagc
      961 gatatatgtc aaaatatcta ctgggtttga agcaacaacg ataataccgt cgaatcctga
     1021 tcccatgatt tcttttacga tttggcgcat gattgacgca tttgttttaa ctaaatctaa
     1081 acgagattga ccaggttttt ggttgatacc agctgtgata acaacgatat cagcatcttt
     1141 acattcttca tagccacctg accatacagc cacattttca tcgccccaag ccataccatc
     1201 taaaaggtcc aatgcttccc cttctgcttt ttcttgattc acatcgatca atactaattc
     1261 attagagatt ccttggttga tcattgcata agcgatactt gttccaacaa aacctgtacc
     1321 gacgattact actttacgac ttgttttttt catgaataaa cagctccttt tcataactat
     1381 ttgttacttt catgaaaagt atagtcttgt ttattcattt tatcaacatg ttaattataa
     1441 tagttgttat tataattttt gatggaaacg aattcctgaa tacgcataac agataaatga
     1501 caaatgttta cacctatatc ccctgataat acaaccaaaa gtttagaact gaaaataaat
     1561 ccaaaaaaat tcgctgcaac gatataataa gacagcaatc gcacctaaaa caattttcct
     1621 tcatatgctt tagtgaaaga ttttcttctt aatgtttatt tctccattca ttgggagtac
     1681 atgttgaata cttcttgaat aatttgaata aagaagtcgt ttgtttatat ccgatacgat
     1741 tagcgatttc ttccaacgaa aaactcgttt cttgaatcag ttccaacgca aagtcgaatt
     1801 ttttctttag aaccagttct tgaaaggaag catcaaaata cattttcaat ttattactaa
     1861 gatagaccgg attgtagcca aaatgagctg ctgtttcttt taacgtacaa gaagtaaaat
     1921 gcttatcgat ataatctaat actctaagca tctcattttc tgcgcatcca tctgctgatg
     1981 aaaagctttg ttgttctaat aaatgagtca attcgatcag taatagtgat gtatagagat
     2041 ttaacgaacg gatcttatga ctgtatgtat caaaataaat caacagcata ttttcaatca
     2101 aatgcgtcaa acgacttgtt tttccgccgc gaaaatgaat gaattgtgta tgttcccctt
     2161 tttcttttga tgcatttatc aaaaaagaag aaagtagatt atttgtgctt gccaaatgat
     2221 tcaatatctc tgtagataag gtttcttctt ttaataagat gttgattaaa atatcctctt
     2281 gccctgtgta ttcgattctt tgagtagctt ctttatcaat gatcaccacg tcactttctt
     2341 ccatctctat cctttctcca ttaataattt gtaaacatgt accgctatac atataattga
     2401 tttcaacaaa atcatgtgta tgctcgggaa cttccgcata tcgacggtgt ttactgatat
     2461 gaatatttcc ctgttgaaat agatcccctc ggtggatttt tggcggtgtc tgaagatggt
     2521 aagctaagtt taaatcattg atattcattc cttgttcacg ctgcagagtt tcgattgatt
     2581 cttcgttacg caaaaaaaat gtctaattca gctagttcca attgactccc tcctttttgt
     2641 aagcggtttc tttcgcttat tagttcacgc tgtactgcta ttatatgcaa agctattttt
     2701 ggtttcataa agactttcta tggttgggac atgaacttcc tgtgattccg ctattgagag
     2761 aataaccact tcatactata tttttgataa gcgcttacaa aaatgaaagg aacatgctta
     2821 tgaataaaaa gatgatgaaa ttttctctgc tgtcagtctc tcttattttg acttctgctg
     2881 gtgcagtcac gtctgcaatt cccagtatgt cagctactta tgccacgcgt tctttatctg
     2941 ctgttgaatt attaacgact gctccctctt ttatggtcac tatttttgtt ttgattagta
     3001 gttttattgc taggaaaatc ggacaaaaac aaactgtgat catcggatta gtgattgcat
     3061 cgatttgtgg tatcctcccc ctcttctcac aaagttacag tattgtctta gcttcaagag
     3121 ctggactagg tattggcttt ggattgatca attctttggc agtcagcatg attgctgcct
     3181 tttatcacgg agatgaacga gcctctatga ttggttttca aagtgcattt caaggatttg
     3241 gtgcagcttt aatgacattt gttgcaggac agctagtaca gttcggttgg caatatacct
     3301 ttttagtcta cgccatcagc ttacctattc ttgtcttgtt tgttttgttt gttcctacac
     3361 ctgaaacaac tgaacttcaa tcgcatggca cagccccaac aagacaagta atcaaaacga
     3421 atcctaaaat gattagttat ggtatttttc tcttgctggt catcgtggta tataatgctg
     3481 tatcgattaa attagccaca gtcctgacga cttattctct aggaaacgaa actaatgcag
     3541 cgaatatctt aacaatcatg caattggcaa gtatggctgc cggagtttgt tttggctggc
     3601 ttcagacaaa agcacatcgc tatatgctta cactttcttt actactgatg ggaatcggct
     3661 ttgtattgat tgccactgtg ataaacctgt atgtgattgg gtttggtgcg atcctaacag
     3721 gaatttcttt ttcgttattc attccttact tattcaacca agtaagtatc caagcgccca
     3781 agtacgcgga gagtttcaat acttcaatct tattggttgg cgctaattta ggtactttca
     3841 ttgcaccata tggtttgctc tttctctctt ttgcttctct tgggagtaaa acacttgctg
     3901 ttttcgtcaa tggcagtatc ttgctgttga ttagcgcgct tattagcctt ctagtagtac
     3961 acaggacgaa tagaaagaca gtgataagag tagatgaaga aataaaagaa caattgacaa
     4021 caaaataaat ttgatggagg tcgtgtaaaa tgacaaatga aaaacataaa gatagatggc
     4081 tttggtatcc tggagacttt gagatccgcc atggactgct acaaaatttc caacgggaag
     4141 aacgaggttt tgactggcca gcgtactggt acatggatga ttgccatcgg aacgtcaagt
     4201 ttaagagata ttactttctt gatcaacctt ccatgtttaa agtgacgatc caaggagttg
     4261 ggtatgtaga aattaatgga caaaaacatc catgtggaaa atggctgact tgtccagctg
     4321 gtaaagcaaa aattcgtatc tttgtaggac atacatcagg gctaccagcg atgtttatcg
     4381 agggagacga agtaaaatca gatattggct ggacagcaag taattttatt gaagaatatc
     4441 cagctggctg gagtccgctt tatgtcgata tcgcaaaaga tccaaatcaa atctattatc
     4501 aaaaagaatg cgtccatcca gtcactgaaa cggagattgg cggaggaatc ttattcgatt
     4561 ttgggcgagc agtgaatggc tgtgtagaac taatatctct ggatgaccat atcgaatcta
     4621 tcactctatg ttatggagag tccgatgtag aagctatgga tatcgattat tgctactata
     4681 agcaaatagg aatgacaaga aaaatgaaag ctcgtaaacg cgcttttcgc tatttgttta
     4741 taccagaggt ttctactgga atggttcaac ttcaagtttt tcatgaattt ttatctcttt
     4801 cgaaacaagc tgctttttca agcgattctt ctttactgaa tcagatctgg tcagtatcag
     4861 aagaagcctt caagttatgt agtgatttgt tttttatcga cggtatcaaa cgagatcgct
     4921 ggatttggtc tggagatgcg tatcaatcat tcatgatcaa tcaatatctt ttttttgacg
     4981 aggaaatcaa taaacgaacg atattagcat taagaggaca aaatgaaatc aagcagcata
     5041 tgaatactat cgttgactat tcgatcttgt ggttgattgg tatcgaaaac cactatatga
     5101 tgacgaaaga ctatgaattt ttacgcatcg tctaccctaa gatgcaatca ttaatgagat
     5161 acttgatgga gcaaacgaat gaattagggt ttatttatgg gagagcaaaa gattggatat
     5221 ttattgattg gtctgaaatg gataaagaag gaacgcttgc tgcagaacaa gtcttattac
     5281 tgaaagccta tcattccatg atcacttgcg gagatatctt agctgaaaca tatggagaga
     5341 acgaaaatta tcatactcat ctctatcaag aaaaatatca actattgaaa caaaacctat
     5401 ttgactactt ttgggatgaa gaacttggcg cttttatcga ttgctatgaa tctgggagaa
     5461 gaaatgtgac gcgtcatgcg aatattttcg ctgttttatt cgattttgct aacaaagacc
     5521 aacagcaatc gatcttaaag aatgtcttat tgaatgacac tatcacacag attactactc
     5581 catatttcaa attctatgaa caagatgctt tatgcaaact cggacagacg aatatcgtct
     5641 accaaacgat tcttgattat tggggaggga tgttatctca tgatgcggta actttctggg
     5701 aagaatacga ccctactcaa acaggaaatg aaaagtacag tatgtatgga gatccttatg
     5761 gaaaaagctt atgccatgca tggggagcaa gcccaatcta tcttttagga cgctatttta
     5821 tcggtttgta tccaacaaag ccaggttatg aagaatttat gatacaacct aatctagatt
     5881 catttgaaac actgaattgt acgctaccta tcaaagatgg aaagatttcg cttgaaaaaa
     5941 caaaagataa gatcgtgatt acttctgata gaacaggcgg aacagtgaaa atgaatggta
     6001 aaagttatcc tcttctagca aaacagccac ttgtcttaac aagctattca aaaataaatg
     6061 cataaaacaa aaaactgctt gcgacatatc agaagacttt aaatcttctt ctgatattgt
     6121 ccaagcaatt tttattttga agtttgctta attctgttct ttagaaacaa tgtttgttct
     6181 ttttccggca aaaagaaagc tagataatac gacaagtaaa aagaaagcga tgattccaac
     6241 tgtcaaggct atttgaccgt attctcctag tttcccaagt acaagtcctg gtactagata
     6301 atcagtatcg gcaaatgtag aagaagcgat tttaagatct cctaatactg gaaggataaa
     6361 taatggcagc caactaatca ataagctatt gaaagcagaa cctaataccg cccctcttct
     6421 accgccagtc gagttgccga agacaccaga agcagctccg cagaagaaat gtcctactac
     6481 cccaggaatg ataactgtag tttgcaagcc tatcatagca aacatggata gtgttcctac
     6541 aaagaagcta acaaaaaagc caattaatac agcattaggt gcatatggaa aaacgatagg
     6601 aacatctaat gctggtttag aatcgggtac gagtttttta gcaatccctt ggaatgctgg
     6661 aacgatttct gccaacacca ttcgaacccc ttgaaggacg ataacaaacc cagctgtgaa
     6721 tgttccagct tggatcagtg caaagattac agcgttgtct ccattgctta gttctctttc
     6781 aacaaatcca ggaccagcta acaaagcaag tgcgacatat acgatcgaca tcaagagcat
     6841 gatcgataca gtagaatctc gcaaaaagct gaaactttta ggaatcttta tgtcctctgt
     6901 agaacggtct ttgtttccaa aaaactttcc gatccatcca ctagaagcat aaccgatatt
     6961 tccggtatgc cctaacgcaa cttgattgtt tccagtgatg ttcttcataa acggttgaca
     7021 aagagcaggc gtaatagtta atagtgttcc ttcgaagacc ccgcctaata agatcatcca
     7081 caaatttgca gtaaaccccg cgcttactaa aatgactgct gttagacaag aaacataaag
     7141 cattgcttga cctgtaagaa aaatgaattt atatcttgtg aatctagcaa gtaaaatatt
     7201 cacgatcatc cctactagca tgatcaaagc agtaggtgta ccgtattgtt gcaaagcaat
     7261 cgccactact gcttcattgc taggaactac tccttgagta tgcagagctg tttggaacat
     7321 cgaagcaaaa ggggttagtg aattactgat taaaccagca ccgccgatca gtactaggaa
     7381 tccgacaaat gttttgatag ttccttggat cacttctgaa aatttcttct tctgaagaac
     7441 gagaccaagc agtgcaatca aagaaacgag gatggagggc tgactgacga ttgacacaaa
     7501 caaattcaac aatttatcca ttgatagttc ctcccttgaa tcatttttat tcttttgaca
     7561 agatcttcgt tactttttct tttacttctt cgctgtcaat gatcgattgc aaaggtacga
     7621 gcttttcatc aggtatagaa gttagcgtat ctttcaaaga atgctcaatg aagaaataat
     7681 cagccatatc gctagataca cttccaacat ccgtatgact tacttcaaac tcgtccacat
     7741 ctgctccaag atctgcaagt gcttgtttga tgttcatctc taccataaaa cttgttccta
     7801 atccactttg acatactgca aaaattttca tatcattcgt ctccttttct tatgatcgct
     7861 aatatttctt cttttgttga tgcgtgtaat aaatcttgaa gattttgttt attagaaagt
     7921 agctttgtta aatttgccaa agcccgaaga tgtgcctcat tatcgatagc agctaaacag
     7981 atgaacaaac taatgggatg ggctggatta tcaagcagat ccacaggttg ttgaagtttc
     8041 agcaaagaca tccctacttc gtttacgcca tcttctggac gagcatgagg taatgcgatg
     8101 tgatcgccaa tatggataaa cgcaccatac tgctccacgc gctcgatcat agctttgata
     8161 taactttctt ctattttatt ttctgataat agcggctgtg cggcaaatgt gattgcttct
     8221 tgccagtttt ttggttgatc cgataattgg atcatttctt ctgttaataa ttcagatagc
     8281 ataggacgtt gatcctcctt cttttctaaa agtttgttca ttttacgatt taatacttga
     8341 tagagctttt cttcgtctac gccttctttt aattcgatat aaggcagtag cgccgacaac
     8401 aattcttggg cagaaggcat tgaaaatcct ggtatcagcc aatctctttg tacttgattg
     8461 atcagctgat ttttttccaa gtcagtcatg attggatgca gaacatagag agactttttc
     8521 ttatcagtaa gtgtgagagg tacagtagaa aaaataatat cgtaagaagt ttcgtcaacc
     8581 tcgtgtaagc ggtcagctga attagtctct gtaaaaaaga tcatcgggaa taattgctga
     8641 agttcacttt ttaagatcaa tgaagagcta atgccacttg gacagacgat cacggctctt
     8701 atttttctat ttctttcttc tgcgtccgct tttctgatct ctccgccaaa tagtatagtg
     8761 aaaaaaccaa tctcttcttc agggatgctc ttccctattc gtctttctaa cggaagtaat
     8821 gcttttcttg tcatttcgta gatggaagca tattgatgct taatttgatc gatcatcaca
     8881 ttaggtaaat aaaaagaata ggaaatacga aaataggcag ggaccaagtg ataaaagaca
     8941 ttcatcaata attcacgaaa cgaagcaaat tctacggcca tcaggaattc catgcgatgg
     9001 atgacttctg aagaacattc cagcaaaaaa tcaagtgcag gctcctggac atctccttca
     9061 acgatcgatg ctaagcaaag agagaaaaat ataatctccg aattttgatg ttcggaatag
     9121 ccaaggccag tcaatacttg tcgactaatg tcagtgactg caagagaagc caacagttca
     9181 gcatctttta ccagttccat ggatgttttt ccaattcgaa tggttgtcaa actcaaataa
     9241 taagctaatt catccattcg atttggtgcg aatcgaagat ctgatagtga aatcgtttta
     9301 tctagcaaat ctctaacttt ggcgtaataa tcaatggaaa gggttgcggt taaatacgtg
     9361 agcccaaatc ttccagcaag tgactgcatc aaacgagcag tgcgattttt tgcttctctt
     9421 cttaatattg cttctgaccc tagcagccta aaaccagttt tcctagtata cgacagctga
     9481 caatcttcca cttctaattc atttcttatc tttttgatat ctgacaggac tgtcccttta
     9541 gaaacctgaa gaaaatcttg aaaatgaaaa acagaaacag acgttgcagt cgaataaacc
     9601 atcaaataaa gaagatctct tcgctcttgt tcggtataca cagcttcaaa tcgagtaata
     9661 tccaaaaatt tttctgccca gtcgattgct cggaatttct ttatacggat acgggtatca
     9721 tccacatatt cggcaaaagg agttaattgt ttgatcgcct cgcttgtatt tacttcgatg
     9781 atctctgctg ctttttctag actgatttct cttggtgttt tcaggagata actaattttg
     9841 tagttcacag cgctgttcac ctcctactta tattctagta tggtagcgct tttaaatcga
     9901 ggggtgtttg gtacaactta cagttggatt ttttttacga ctgtaccgaa cgatttctat
     9961 cgaatggata cagaaaaaaa gcaggtgacc aaatgtctcc tgcttttatg aattgatttc
    10021 gtccgatcaa tgatgatcat gtttattttt tgaggtcagt tcttcaaatt tttctttgac
    10081 atcttcaacc gctttttcga tatctttttc aaagtgattg ggctcattag gctttgtttc
    10141 ctcttctaca gaatttgaac cagataaagc tgtttcttta ttttctgttt caccagagtt
    10201 tgtgccagct aatgattctt ctttgtcttc tgtttcacca gaattagttc ctgctaaagc
    10261 cacttcttct tctgttttag gggccattga acctcttaat cgtttgtgtt tttcaccgtc
    10321 ataatttgtc atgttggttc ctcctagtag ttctatactt tttcatcgta caataagaca
    10381 gtttatgcgt caaaaaacaa acccgattct tacaaatatg tcatattttt ccttctgatt
    10441 cttgaatgct tttgattcct tcttccaaaa ttttcatttg taagattgtt tcttcatctt
    10501 tgatgacttt ttctgcgcca ttaactaatg tctcgtaaat cccttcgtag acacggctgt
    10561 aatcaccaac ctcggaaatt actttttctt catgataact cccttgttca tctatatagg
    10621 tcaatacacc ataatgttcg ggcaaatcga taccaaagtc ttcatgatca ggcatgtaaa
    10681 acattttcag atgttcttct tgtcgatctt ttgtttgttt cacgaacatc cctttttttc
    10741 cgtaaacgac gaaacttgga cgttctttga tgcggaaata actggatttt atcgaaattt
    10801 tcatatttcc atagaaaaaa tccagatcga agtaatcatt cattcgtcct ttccctaaaa
    10861 gttgacgtac atcatagctg atatcatctg gttcgccgaa ataagaaaga acttgatcta
    10921 ctgtatgaca agcatgtcca tataaatagc tgttcgcagt agaaaattca tgcgtatttt
    10981 ctggtacttc cggtctgaaa taatcataat gcatttctac ttctaaaata tcccctaatt
    11041 ttccgctttc gattactttt tggactgtca agaagtcaga atcgaaccgt ctgttttggt
    11101 aacattggat aaatagacct ttttcttttg ctaatgcaaa taactcttct gcttcctttg
    11161 acgtttctgc aaaaggtttc tctactagta cattcttacc atgttcgagt gccatttttc
    11221 catattcata atgaaattga ctaggtgtag tgatgacaac taattggatc tcaggatcat
    11281 cccaaagatc agacagctga tcagtatatt cgatgtcttc gaatgttttc cacactggtt
    11341 cagatgggga aaaaattttt ttgatattga ttttgttcgt aagttttctt gaaaaaggca
    11401 aatgatagcg atttgtactc tttccattgc caagataagc gattgtaagc atgagtggtg
    11461 cctcctttta ttgatgtttt cagtatatcc tagaaaagcg cagaaagtat ttataatagc
    11521 tattctggta aaaaagttca gaaaaacact tgttatcatt cggtttggtg aaattaatgt
    11581 tcaaagtaag gataaaaatc aaaaagatag tagcggaacc aagtgtttat cagtaagata
    11641 gaatttggag gtcagatcat gcttattgaa gaaaaactaa caaaacaaga gttgtttacc
    11701 acgacagaga aacggattgt cgattacatc cgtaggaata tcgaagctgc agtctacatg
    11761 acgattgaag aattagcaaa agctacgtat acttcccatt cagctatcat tcgtttatgc
    11821 aaaaaaatgg gattcagcgg atttaaagaa ttccgtttcg agctggggcg ggaagttcat
    11881 caattgattg ctcagttcaa tcaaacgatt gatcctaatt ttcctttttc aagtgaagat
    11941 agcactcagc aaattgcaaa gaaaatggcg gatttatcga tccagtcaat aaaaaaagca
    12001 caattgcaga ttgaacatga agatttagat caaatcggta aagtattgaa caaggcacag
    12061 cgaatctttc tttttgcaaa aggtgattct caaatcacgg caaggaaatt ccagaataaa
    12121 atggtcaagt tgaataagtt tttgatcatg gctgaagaat acagtgattc ttcttggaat
    12181 gcagccaatt tgactagcga ggattgcgcc atttttatta gctacagcgg tcgcatccat
    12241 cattatgaac gaataatgac ctatttgaag catgtagagg cccctacctt gctgattacc
    12301 ggaaatcagc attcagaaat ggccaaacag gctgatatgt gtcttgttat atcgcaggag
    12361 gaatatgatt ttgctaaagt agcgactttc tcctctcaaa ttgccttcga ttacgtatta
    12421 aatacgttat tctcagtgat atattctcaa aattttgaag agaatatccg caacttgaaa
    12481 aacaaacaag aattgatgca aaaaggcctt cttaaagaat atcggaaaac ttaagaaaag
    12541 atcctcattt agtaataaga aagatagtgt ttgataggct ctctttgata tcatcataaa
    12601 aacaggctga agttttcttt ttcaaacctc agcctgtttg tttacggaac gattcgaata
    12661 gatatattat agaaatgagg aatagctaac ttctgtttct tattttgaac gattgacgaa
    12721 aaagatatat aagaaggagg aaaatcagta tacatgcagc taacaatcca tacaggaaaa
    12781 atcgtttgtt ctcggatact ttctgtactt ctttttgaac ttcttttgca tgataaggaa
    12841 cccggtgccc tctgacaagt aagcgatgac tgttgaccat ataaggtgta caagttagga
    12901 gtgtcacgaa atcctgccct ttttcgattc tcagatcttt tgtatcagtc ggttctaccg
    12961 tcttgatctg atcgacttga taagcgagtg tccgtccatt gatttcgata tagaaagaat
    13021 cacctaaagt cagtttcggt aagtctgtga acaactttgc ttgaggcagt cctcgatggc
    13081 tggaaataac ggcatgtgta ttttctcccc ccgtaggata agacgttcct tccaataatg
    13141 aggcaccttt ttctagcagc acagtattcg tttcatcaaa aattggcaaa tggacattga
    13201 tctcagggat cgtcaaaata ccgatcgtat gtttctcaaa ataggttcta tcctttttcg
    13261 caggttcttt tttcttagta aagggatctg cacctggatt tccaccttct tttgctaatt
    13321 gctggttttt ctttgtcatg ttttcttgga ttttagccat gacttcttca ttttctttga
    13381 ccgcttgttg ctgataatga gagatgatct gctgatccag ataattattc agcgcgtcgc
    13441 taacaaatgg ataagctagt atccctattc ctgagagaag caagaaaagc atcagcagat
    13501 ctagaagttt ccttttcttt ggtttcattt gtgttctcct tgtgatgact gtttgataat
    13561 aaaagaaaaa ggagcatttg cgtaatccgc tgctcctttt tccaattctt gcaaaaagac
    13621 taaatctgac tgtgcttgcg tctagcaaag tacagtccag caatcagtag aaggactact
    13681 cctgcaccga tatacacata gattccctta ccgcctgttg aaggaagtgt acctttgtgt
    13741 ttatttggta ttttttcagg agaaatcaac tgtcctgctg ttacataaga ttgttcatcg
    13801 atagtaaatg ctacacggtt tgttaatgga acatattttt ctggcgcttt cgtttcttcc
    13861 agatagtacg tgccatattt tagacctgtc acatcgatta aaccgttact tgtggttgta
    13921 aaaaccgttg ctgattcttt cgccgatacc cagctgacgg cttttgtgga tgggtcgatc
    13981 gataaatatt tcgctgtgtc actatcttga tcacgaacga cgaattctgc tccagcaagt
    14041 gtttggtctg atgtaacgtc accatctact ttaacaaatc gtttgccccc agtaacgaca
    14101 tcgactgacg gtggtgtttg atcatttgta tgcccgttat cgacatttgc ttggttagaa
    14161 aatcctttgg ttggatctgc tttttcgttc aaatgcatat agtaaacgaa tttcaatgta
    14221 ccgccaggag ttaatgaagg aatataattc ggatcaactg aaaccgtgaa tccgtctgtt
    14281 tgctcagtga cagaataatt tactgggtcg atttctttat ttccatcata taaggcataa
    14341 gcatacgttc ctgaagaatc attatcaaat gttaaagcag cgtcatgagt atcgataagt
    14401 ttgaatgttg tgtacttgtt ttgcgttcct tctttatcag cgatacctaa tgggatattg
    14461 acagaaatct catattgaat tttttcaccg attgcgacat cttttccatt caattgaggt
    14521 gttgttttat caactttaga cgtatcattt ttgatggtct tttctactgg tgtttggcta
    14581 tttgccagga tctcaaaagg cgtgattgtt tgcttttcaa tcatttccgc attatcagga
    14641 gcttttactt cttctaaatt atattttcca acttctaaat gattaacgat caactggcct
    14701 ttttcaatag atgcctcggc aaagtcattg ttgccgatgt cataagaatg accagtaatg
    14761 aaatgtttgg ctttggtttg atcagttgtc caagtgtaca atccatctgt gacactttgg
    14821 atgtattttt tgacgcttgg tgttccttct tctttagaaa taataaattc tgctccattt
    14881 agtgcttcgt tttcggcagt accgattttt gtaactttca acgttccatc attaccgact
    14941 gtatttttag ggtagagatg gatagtatct agttcttctg tcccgtattt ataagagcca
    15001 tctgcttgtt tgatcatctc atatacaggg aaagctaaaa ccatgtttgc ggcagctgac
    15061 actccgtctt ttggttcttc tttgatcgtg tagactgcat ctttcccatt ttgtttttta
    15121 ggtaaagata aagtgacatt tccatcagca tctgtcgttc ctgaagcaac tggtgtacca
    15181 ggagtcaaag actggactgc ttgttttgct gcatccacgg acgctccttt atctcgttgc
    15241 gcataaaatt cttgagtgac gttataaact gaaaatgaaa tatcggctaa tccttggtat
    15301 tgatcgaatt cgctcatttc tttcccgctg ttttggatta aaggatcggg taaatcagtc
    15361 attttctttt tatgaaggat gacttgagct gctgtctcct ctgcaaacac tttttttgcg
    15421 ccgaagctgt ttgtgagtaa tggtaaaata aggaaaagga ctcctaacat aacgtttatt
    15481 tttttatgat ttttcatttt ttcactcctg ttcttttttt gataaacaac cagattgcaa
    15541 agcttgcgaa taccatacca acaacgccta aaagtgtatt tttcgtttcg tttgtttttg
    15601 gaagaaatgg acggctattt tcttttttat tgactacttt caatatactg tccgcctctt
    15661 tatcctcaga aagaaaagtt gaaatggtaa acggaatttc tgtttcactt aaaatataac
    15721 cagaaggtgc ttcgatctct tttaatcgat aacttcctgt tttcaacccg gaaattgaaa
    15781 aagcgccatt tttatcggaa atcaggtgaa gcgcatctga ttcgttcttt gtccacttgt
    15841 atccgttggc tgtttgctgg aggtaatttc ctgctttgtt tttaataagg aatactgcgc
    15901 cagctaatcg tttatctggt ttatttgtct cgattttgac aaattgctta ctccctgtat
    15961 atacagagac tttctttgta ctgacaccaa aaccaaaatc cagtgttccg ttgttgttga
    16021 tttccttgtt cgcctgtgct gtactgctta gctgcatctg ataagaaatg gtcagttttt
    16081 ttccagcata tttttccaac gaagggatcg aaaataaaac atcaaatccg tgaggtttct
    16141 tgtgtatgac aaagccttct gtcagctttt ctccattcga tgaaatcgtt aaactattag
    16201 gtaaaaacgt taatgcttca tccgcactat ctgacaattc gaacttttga tagtcaagga
    16261 tatttacagg aattgtcgta tgcaaggaat aagtgatttt ttcaccaatc gtaaaatcat
    16321 ttctaggctc ttctaatacc ttctcaaaag gtggatcagg gtagtcgttt tcttcatttt
    16381 taggatacaa atggatagtt gaaagttttt gattgttttg tccatgtaca ggaagaacaa
    16441 ctaccatatt ctctgccttt tctttgacga cttctggtac tttggattca atgaaaagat
    16501 acgctttatc tcttttttct tgatctttag cggccagaga aaaagaagcg ataccatctt
    16561 cgttgttaag agttgtagtt gttgcttctg cggtaaacat accggaagac gcaccgtttt
    16621 ttgcgatctc tgcttgtgct tcttctaccg ttttgccctt ttcccgtaga tggtaaaaag
    16681 aatctgtgac atcataaact tggaatgtga caccatttaa tcctcgatac gtttgtaata
    16741 aagctttttc ttgtccgtca tttggatgat ttttcggcag ttgcccgttt gggaaaagca
    16801 atttgtgtaa agtaatctga accatttctg tttctgtttc tgttgctacc tgagtaaaag
    16861 ctggtttaaa tagtaacaag aagagacaca tactaagcca accaagtttt ttcatattta
    16921 gctgccccct ctttcttttt tcaaaaaaag ataccaaata gaaaacgcac aaccaatcat
    16981 ccctactaga tagattccta aacggccaat tcctcccgtt tcaggtaatg gtacttttgc
    17041 ctgattcgtg atgtctaaag aaatctggtt gtttttggct cctggtgaca gaacagattc
    17101 atgatcttgt ccatccactt gaattgaccc atcttcgtgt ataactatag taactggctc
    17161 ttttagacct tggtatcctt ctggtgtaaa agtttcggtc agcgtataag ttccaggttt
    17221 taaattttcg aatagaaagg tatctgtttc ttgtccatct tttggcgatt ctaagtccat
    17281 ttctggtccc tgcagtctga attttgctcc ttttaatgtc tgtccgttgt cttctttttt
    17341 gtgtaccgtt agatcaaacg cttttagtcg attttgatga gtgagcgtcc atacattatt
    17401 tgtcatctcg gtattttttc ccgtgtaaat gatttttcca tatttatcga tttggaattc
    17461 ataatttccc ggcgaagtat catatccggc aggaccagct gtttcaacga ctcgatattt
    17521 accaggtgtg agtgaatcaa aactggcaag acctttttca ttcgttgttt ggctgtcaat
    17581 tggctgataa gtaccatttg cttctttttt ctgtagggaa aatgctgctc ctttaagatt
    17641 tatctctttg tccgtccctt gcatcgcata cttacgtact gctacaggaa cttcaacaaa
    17701 aggattttca atagtcaact ggatcgtatc atcggaagta gtgactgttt ttccatcaat
    17761 ggttaccgta ccatcagaag cgatcttgat ttcccaagta gtctttttgc ttaatccatg
    17821 cccttctgga gcttttgttt ctgtcagcgt ataggtcatt tctttttgca atttgacatt
    17881 ttctggaaga gaataggttc cgtcgccatg atctgttagt aaagtatcaa tagaatctcc
    17941 tgttaatttg aaaacagcgc caataagatc cttttcacct gtagaggaat ttttcgttat
    18001 tttcaagttt aacggtgtga attgtttagt atttttccat gtcattgcat ctatttgttg
    18061 agaatcgtat cctggtacag gtaattcttt gattgtttgg taactgaatg cttgaccttg
    18121 attattgtat tgaggtagtg ataatatctc ttgataactt tctccgctat ttgcagataa
    18181 tttgtcaatg tctgcacgtt cccacgtatt tgttgtatct ttagctggtt taatgattcg
    18241 aatatatccg tttttccaag ctgcagcatt tgtcgtatgt tcccgttgaa tctcaaaagt
    18301 aacttgatct ggacgatcag ctagattgtt gtcaaattct tcccataact ttttgatgtg
    18361 aagactgact ccgggagctt tagcagatgg tataccgaat tcagctaatt cattggtatc
    18421 aatgtttggc tggaaagttg tcctgccgtt catttgatac cagaaatttg gatggaagtc
    18481 ctcattttct gtttggattc tcacttggta atggatttgg atttcttggt cttttcctaa
    18541 atagatctga ttgctcttga tggtatttcc ttctatggaa atagatggag tgaccgttgt
    18601 aggacttgtc cccacactct tgactgaaag tgtaccaggc tgataaatga atggttctgc
    18661 gattggatca tttatttgtc cattgcttac agttgctgag atctgtacag cttttttggc
    18721 taggtattca gagatatctg ttgcatgatc agctgattca tagtaaagat cgcctttttc
    18781 atctgatgaa accatttgtc gcatacgaga ctctacttct gcttttgaga gaccagctgc
    18841 cggatcgctt tgaagttgga tgccaagacc atgtatttcg attcctcgtt ctttgagtgc
    18901 catcgcttct ccgatggttg cgataaacgt actgtcgatt cttctggata gattgttttg
    18961 gtcaggtgca tcatagattc ttgagattag agaagtattt cccggccgat cttgcgtatt
    19021 agaaaactga gttccgtaat aattgctgct tgattgtgcg tgtacccgct gtactttata
    19081 ggaaaatgtt ggtacaccat ccgtcagcaa aacgatcact tttttatgtc catttggaac
    19141 ggatagcatg cttcctgcat ctcttagtgc tttttgtgta aaagtaccac catttgtccg
    19201 tgaaggtgta atggatttta cttgattttt cactgaatca aatgaaccca tctgtactgc
    19261 accgttactg tagctatatc cttcgcttga gtagccgaca tatcccatat tgattttgtc
    19321 tgtgataccg ctatctgcta aagtatcgac aaaacgatcg acaccaatct ttacttcacc
    19381 gatccgatta ttgtcgttca tacttcctga ccagtctacg accaatacga gatcaagagg
    19441 cgtgatatct ttttgtacat ttccacgagc attcaaataa acatcaaaca atccgggtgt
    19501 acttgtttcc tttgcatact ttcgtatcgc atagtctgct tgattaccgg ttcctccata
    19561 ttcgatatac gaatgggtcc gatcattagg atctccattc caagaattta taccatccca
    19621 ttgatttgtt ccgtttttat tgccttgatg atcaagaaca tttgtatttc ctgtaggttg
    19681 ccaactggct ttaggataag tgccctgatc atctgttgta tattctgtag gaatcgtttg
    19741 attagatggc acactcacag ctggcaagga taatgtaggc aatgtaaatg ggctgacaga
    19801 attttcgttt tcttttgatg attcttcttc tatttcgtct ggctttggta ctgacacatc
    19861 tgtatctttc ttattttctg tctcatcaat aatgtcatat tctttattat cgacgatcag
    19921 cttgtttgcc tggctgtggt aaagtgctgg atcgattgga aaactcagat cgatagtttg
    19981 tttagtgttt gatgagattt ccaacgtaat tttcccgcta tttacctgat aagcatagcc
    20041 atctccttct tttctttcca catcacttaa gtaattttcg actagaccaa tttctttttc
    20101 tatcttttca gcactggtgt tattcgcatt tagcttgatg ttcagtgctt tttttccaga
    20161 ctgatccgaa atgacttcag ctgaaattgt ttctaaaact gttggatcat ctgtagtgtc
    20221 tgctattgca gataatggca caaagtttga taaactcaat attatcagca tgaaaataac
    20281 tttcagtttc ttccctgtgg tcgtcatttt tcccctcctt tttctttttt tctgttttga
    20341 cgtgaatagt gaaaattatt aaaataaaca ggcgttacat aattttattt attttaatat
    20401 gataattatt atacgaaggg cgctaaaggg aatcacacca attttcatac caattggttt
    20461 catttatgaa gttgattcct tcgattaatg agcgtttcat tgaaagaaaa gggttgaggg
    20521 agaaaaacat atgatcaggt tgttggaatg aaactataga aagatggaaa aaatcattcg
    20581 aattctgtcc atttctgaca agtggatgac taaaaaagaa cttgccaaac gaattggtag
    20641 ttcagaatct acatttgtaa ggtatatcga agagatcaaa atccgctggg gaacggatgt
    20701 aacgattcaa tcttcacaaa aattggggta tcgtcttgaa catttgaatg taccagctta
    20761 tttaaatgtt ctgatggaaa tgtcgcaaag ttctacaaac agtcgtttat taaatgagat
    20821 tattgtcaat ccgggtaaaa cgattgagta ttattgtgaa aaaatttcca tcagccggtc
    20881 ttcttttaca cgtaagttga aacaatgcaa tcaaatttta gaaagctatt tattaaaagt
    20941 gatcgttgac caaggttttc agttaactag ccttaaaagt gagctccagt tgcgtatctt
    21001 tgttaccttt ttctttttaa gttactacgg aagacgcacg ttgccatttc caatcgaaaa
    21061 aaagaagatc aaagaaatta tggtcaagaa taattgtcag atgaatttgt ttcaggaaga
    21121 aagcccatat gagcaatctt tctttattat gtattttgtt gtgcatctca tgagagaatc
    21181 ccaaggaact ccatttgaat ggaaagctgg agactcttat gtacagttga taaatatcga
    21241 taaaaaagac ttcttaatct tgaaaaatct cttaggaaaa ttgaagtaca gcagctatat
    21301 aaaagtagtg aactattttg tcaatacgat tttttcagaa ttttcggaga aagaaaaaaa
    21361 tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat ccgctgattg
    21421 caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt ttttctcccg
    21481 tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg cacagcagat
    21541 gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat tttattccac
    21601 tctactgaat cagaatctga tttattattt atctagctat atcttttgga tgcttatcgg
    21661 taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca gcgacttaag
    21721 tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg tgcataaaat
    21781 tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc ttctcgagta
    21841 tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata tattgattga
    21901 tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt tatgaataat
    21961 caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata cacatagctg
    22021 tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca
    22081 actcttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca
    22141 tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc
    22201 ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg
    22261 ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt
    22321 gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt
    22381 tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat
    22441 acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca
    22501 tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca
    22561 gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg
    22621 gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg
    22681 attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga
    22741 tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca
    22801 tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct
    22861 gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact
    22921 ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct
    22981 aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca
    23041 actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata
    23101 aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag
    23161 tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg
    23221 aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca
    23281 taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta
    23341 acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt
    23401 ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa
    23461 tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa
    23521 tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa
    23581 aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta
    23641 ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag
    23701 caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc
    23761 caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt
    23821 ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag
    23881 aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga
    23941 cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt
    24001 aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga
    24061 tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg
    24121 cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc
    24181 tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta
    24241 attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc
    24301 aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga
    24361 atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat
    24421 agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg
    24481 acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt
    24541 tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa
    24601 aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga
    24661 ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc caataatata
    24721 aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg
    24781 ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg
    24841 aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt
    24901 ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat
    24961 tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa
    25021 cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta
    25081 aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa
    25141 ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca gatatttgac
    25201 cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac
    25261 ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc
    25321 ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag
    25381 tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat
    25441 tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat
    25501 aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt
    25561 tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc
    25621 atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa
    25681 taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa
    25741 tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt
    25801 ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt
    25861 agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg
    25921 tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat
    25981 gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga
    26041 attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa
    26101 tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct
    26161 cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct
    26221 ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga
    26281 gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc
    26341 aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata
    26401 tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat
    26461 tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg
    26521 attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc
    26581 aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc
    26641 acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct
    26701 aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt
    26761 ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca
    26821 acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt
    26881 gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt
    26941 tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc
    27001 tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa
    27061 gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct
    27121 accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca
    27181 tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt
    27241 ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc
    27301 atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg
    27361 ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt
    27421 gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag
    27481 ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga
    27541 tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt
    27601 ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct
    27661 ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg
    27721 atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc
    27781 ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctcctagc
    27841 aaatagattt atgattggta tattgttact tataaattta ttctatagga gacgattgtt
    27901 ttctacaaat atttagattg gaatttgatt aaaa
//
LOCUS       DACDTO010000027        27585 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDTO010000027 DACDTO010000000
VERSION     DACDTO010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.027"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            71..1075
                     /locus_tag="IWK70_001236"
     CDS             71..1075
                     /locus_tag="IWK70_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613800.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            1076..2020
                     /locus_tag="IWK70_001237"
     CDS             1076..2020
                     /locus_tag="IWK70_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613801.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            2020..2982
                     /locus_tag="IWK70_001238"
     CDS             2020..2982
                     /locus_tag="IWK70_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613802.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            3009..3923
                     /locus_tag="IWK70_001239"
     CDS             3009..3923
                     /locus_tag="IWK70_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613803.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            3949..5730
                     /locus_tag="IWK70_001240"
     CDS             3949..5730
                     /locus_tag="IWK70_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9613804.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            6078..6764
                     /locus_tag="IWK70_001241"
     CDS             6078..6764
                     /locus_tag="IWK70_001241"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9613805.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            6784..10365
                     /gene="smc"
                     /locus_tag="IWK70_001242"
     CDS             6784..10365
                     /gene="smc"
                     /locus_tag="IWK70_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9613806.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            10374..11183
                     /locus_tag="IWK70_001243"
     CDS             10374..11183
                     /locus_tag="IWK70_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9613807.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            11194..12192
                     /gene="ftsY"
                     /locus_tag="IWK70_001244"
     CDS             11194..12192
                     /gene="ftsY"
                     /locus_tag="IWK70_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9613808.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            12379..14646
                     /gene="gshAB"
                     /locus_tag="IWK70_001245"
     CDS             12379..14646
                     /gene="gshAB"
                     /locus_tag="IWK70_001245"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9613809.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(14735..15187)
                     /locus_tag="IWK70_001246"
     CDS             complement(14735..15187)
                     /locus_tag="IWK70_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9613810.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            15337..16287
                     /locus_tag="IWK70_001247"
     CDS             15337..16287
                     /locus_tag="IWK70_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9613811.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            16280..17239
                     /locus_tag="IWK70_001248"
     CDS             16280..17239
                     /locus_tag="IWK70_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9613812.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            17236..17991
                     /locus_tag="IWK70_001249"
     CDS             17236..17991
                     /locus_tag="IWK70_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9613813.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            18014..18967
                     /locus_tag="IWK70_001250"
     CDS             18014..18967
                     /locus_tag="IWK70_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9613814.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(19611..19907)
                     /locus_tag="IWK70_001251"
     CDS             complement(19611..19907)
                     /locus_tag="IWK70_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9613815.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            20262..20618
                     /locus_tag="IWK70_001252"
     CDS             20262..20618
                     /locus_tag="IWK70_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9613816.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            20628..21527
                     /locus_tag="IWK70_001253"
     CDS             20628..21527
                     /locus_tag="IWK70_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9613817.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(21924..23003)
                     /locus_tag="IWK70_001254"
     CDS             complement(21924..23003)
                     /locus_tag="IWK70_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9613818.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            23433..24164
                     /locus_tag="IWK70_001255"
     CDS             23433..24164
                     /locus_tag="IWK70_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9613819.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            24309..26336
                     /locus_tag="IWK70_001256"
     CDS             24309..26336
                     /locus_tag="IWK70_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9613820.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            26444..27541
                     /locus_tag="IWK70_001257"
     CDS             26444..27541
                     /locus_tag="IWK70_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9613821.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         9015 a         4938 c         5924 g         7708 t
ORIGIN      
        1 gatttattct gtgtaatgtc cttttttatt aagaaaaact tccatttagg aagcagagag
       61 gggtattcga ttgtccgata accaattatt agaagttcag aacctacata caggattccg
      121 actcaaagat gactattacg atgcagtaga tgacgtttca tttactttag acaaaaacga
      181 gattttagca atcgttgggg aatctgggtg cgggaaaagt accttggcta ctacgattat
      241 gggattactt gatcctaata acacaaaaat tacaggtgag attttatata acgatctgaa
      301 tttaatcgac ctgaacgaaa cgctctataa taaaatccgc ggaaatgata tcggaatgat
      361 tttccaagat ccgttgtctg cattaaatcc tctcatgaga atcgaagacc agatcaaaga
      421 aggactttcc tatcacacaa agatgaatgc agaacagcgt caagctcgtg cactagaatt
      481 gctagaacaa gtagggattc ctaatcctgc gagagttgga cgccaatatc cccatgaact
      541 atcaggcggt atgagacaac gtgtcatcat cgcaatcgca atcgcctgca aacctccaat
      601 cgtcattgca gatgaaccga cgacagccct agatgtaacc atccaagcac agattcttga
      661 tttactgaaa gaccttcagg aagaaacaaa aacagggatc atcttgatca ctcatgactt
      721 aggagtcgta gcagaaatgg ctgatcgagt ggcagtgatg tatggcggac agttcgttga
      781 agtagcaggt gtaaaagaac tgtttgatca tcctaagcat ccttatacac gctctttact
      841 gaactctata ccacaagaag gaaatcatga tgcagaactc catgtgatcg aaggtgtcgt
      901 tccttctctg aaaaacatgc ctcgtaaagg ttgtcggttt gcaccacgta ttccatggat
      961 accagcaagc gatcatgaag aagagcctat ccttcatgaa gtcgcaccga accacttggt
     1021 acgttgctcc tgctataagc atttccactt tagagacgaa aaaggagagg tgtagatgac
     1081 agaattaatc acaatcaaag acctgaaagt tcattaccct attcgcagtg gattcttcaa
     1141 tcgagtaacg gatcatgtat atgctgtaga tggtgtggat tttattatcg aaaaaggaaa
     1201 gacatatggt ttagtgggag agtctggctc tggaaaatct actacgggaa aagccgttgt
     1261 gggattagaa aaggtaacag ccggacaaat catgtatgaa ggaaaagatg taacgaagcg
     1321 aagcaaccgc aaaaaaatgg gttataacaa ggatgtgcag atgatcttcc aagattcaat
     1381 gtccagtttg aatcctaaaa agcgagtgtt ggatatcatt gctgaaccca tccgaaattt
     1441 tgaacggtta agcgatcaag aagaaaagaa aaaggtaaaa ggactgctag atatcgttgg
     1501 gatgccggaa gatgcgctat ataaatatcc tcatgaattt tcaggcggac aacgtcaacg
     1561 tctaggagta gcccgtgctg ttgctaccaa tccaaaactg atcgtagccg atgaacctgt
     1621 atctgctctg gatctatctg ttcaagcgca agtattgaat ttcatgaaac gaatccagca
     1681 agaatacggc ctcagctatt tattcatttc acatgattta ggtgtagtaa aacacatgtg
     1741 cgacaatatc gcaattatgt acaaaggtcg atttgtagaa atcggtacaa gagaagacat
     1801 ttataatgac ccgcgacata tctatacgaa acgtctgctt tcagctattc cgcaaatcga
     1861 tgtagaaaat agggaaagcc acaaagaaaa tcgacgaaga gtagaacaag agtatatcca
     1921 aaaccaaaaa gaatactatg atgcgactgg tcgagtctac gatttgcgta cgatcacaac
     1981 tacacacaaa gtggcgttga aagatggagg tgcaagctaa tgtggaaaac gattctgcga
     2041 cgtgttttat tgatgatccc gcaagttatt attttaagtg ttctgatctt cttattagcc
     2101 caagcgatgc cgggggatcc atttaccggt ttgatcaatc caaatcagga tccggcagtg
     2161 atcgaacaga tgcgacaagc agctggcttg aatgacccat ggtatgtcca gtacttccgt
     2221 tggatcggaa atgctttgca aggcgatttt ggtcaaagtt tcttatacaa acgctctgtt
     2281 gctgacttga tcggcgaacg gatcatcaac actttatatc tatcgatctt gacagttgtc
     2341 cttacttatt tgattgctgt gcctctgggg atgcttgctg gccggtatca aaattcaatc
     2401 ttggataaga ttgtcgtaat ctacaacttc tttagttttg ctgttccttt atttatcttc
     2461 ggcttgatca tgctatttgt ttttggttat cgtttaggct ggttcccaac aagtggatca
     2521 caaacactag gtttgcaagg tggatttatc actcaatgga tagaccgttt gaaatatatt
     2581 ctgttaccat caattaccca agcatttttg gcgactgccg tcacaatcca atatctgcga
     2641 agtgaagtaa tcgattcgaa atcgctcgat tttgtcagaa cagcccgttc caaaggggta
     2701 ccgacgaata aagtattcag tcgacatatt ttccgaaatg cagcgttgcc gatggcttct
     2761 caaatggggt atgaaatcac ttcgttgatt gctggttctg tagttatcga aaagattttt
     2821 ggttatccgg gagtcggaaa actatttatt gattccatcg gccaacggga ctataccgtg
     2881 atcacatcct tagtattgat tttaggaatc gcgacgctag tggggacatt actttcagat
     2941 atcatcatga gtatcgttga cccgcgtatt cggattcaat aaaaccaatc agaaaggaga
     3001 atggatcgat gagtgaaaaa aacaatacag cagttcaaga aagtattcct cctatgggga
     3061 tacgaatgat tgctcgagaa tttaaaaaag ataaaattgc tattttttca ttaggtttgc
     3121 tagtggtttt attattagtg atctttatcg gtgcgatgct gaccgatcaa gacaaagtga
     3181 tgtatgttag tatcttcgac aaatacgctg caccaggaac agtttcgaca caaggacaaa
     3241 agttcattct gggagcagat gaaggcggac gtgatgtatt aggacaattg atcataggtg
     3301 ctagaaactc tgttacgatc ggatttgcga tcacgatcct tacttcgatc atcggggttg
     3361 gattagggat catctctggc tactacggag ggatcattga caacatcttg atgcggatcg
     3421 ttgattttat catgattcta ccgatcatgc tgatcattat cgtcttcgta tcgattattt
     3481 ccagatacaa tgtctggtcc tttatattca tcatgagtgc attttattgg gtagctaaag
     3541 cccgattatt ccgaagcaag acattatctg aagcaagaag agattatgtc agtgcctcaa
     3601 aaacaatggg aaccaatgat tttaaaatca tgttccgcga aatcatgccg aatctaagtt
     3661 cattaatcat tactaatttg acgatgaact ttgcggcaaa catcggtatc gagacaactt
     3721 tgactttctt aggtttcggc ttgccggcaa atgtacctag tttaggtaca ttgatcggtt
     3781 atgcaagtaa cggagaagtc ttagtgaatc ggcaatggat ctggttaccc gcatcaatcc
     3841 ttattttagt tttgatgttg agtatcaatt atgttggaca agcattcaag cgttctgcag
     3901 atgcgcgcca acggttaggc tagtatagaa gcgaaggggg aaataggaat gaagaaaacg
     3961 atttttggat ttgcagcgct gatgtctgct gtcgttcttg cagcatgcgg gggcggaggt
     4021 acagatacct ctggaaacaa ctctggaggc aacggcaata acaaagagga attaactttt
     4081 ccaattgcaa caaaaaataa tgatgatgca atcgaagacg gcgaattaga agctgcggtc
     4141 gcaacggaca gtcagtttaa aggattgttc cagtgggagt tttaccaaga ttcatatgat
     4201 gcagctttta tggctccttc tcatgaaccg ctatttgcaa acgatgacga tttcagaatc
     4261 acagacgacg gagctgcaac gcttgatcta gatgaagata cgaaaaaagc gacaatcaaa
     4321 gtaaaagaca acgtcaaatg gtcagatggt gaagatgtaa cggcggacga tgtgattttc
     4381 ccttacgaaa tcattgggaa taaagattac actggtatcc gttatgacga cactttccgt
     4441 aacatcgtag gtatggaaga atatcatgat ggaagtgccg acacaatctc cggaatcaaa
     4501 aaaatagacg acaaaacagt tgaaatcgaa tataaagaag taaacccaag catgcttcaa
     4561 tcgggtggtg gtatctggtc atatgcagca ccaaaacata cgttagaagg tgtagcaatc
     4621 aaagatatgg aatcaagcga tcaagtacgt aaaaatccag tgacttttgg cccttattac
     4681 atgagtaata tcgttgcagg agaatcagta gaatacttgc caaacgagca ctactggaat
     4741 ggtacgccta aattgaaaaa gatcacgatg aaatctgtac cgatggcaag tgcaactgaa
     4801 gcattgaact ctaaattata tgatatgatt ttccaaatgc caacagatac ttacgatacc
     4861 tataaagaaa ttgctggcta tacaaactta ggccgtgaac aaacttctta tacttatcta
     4921 ggattcaagt taggtaaatg gaatgcagat acaaagagtg tggaatacaa tccagattca
     4981 aaaatggctg ataaatcatt acgtcaagca atgggctatg cattagataa taatgcagta
     5041 ggtgaacgtt tctatgcagg tttgagaagc aatgcgactt cattgattcc tccagtattc
     5101 cagtcatacc atgatgatga gatgaaagga ttcacacaag atctagacaa agcgaaaaaa
     5161 ctattagatg atgctggcta caaagatgtg gatgacgatg gtttaagaga agacaaagat
     5221 ggaaataaac taacaatcaa ctttgcttcg atgtccggtg gggaaacagc gcaaccattg
     5281 gcagattact atatccaaca atggaaagaa attggtttgg atgtccaata cacaacaggt
     5341 cgtctgatcg aattcaattc tttctatgat aaattagaaa acgatgatcc tgaaattgat
     5401 gtttaccaag ctgcttggaa tacaggaact gatccaaatc caagtggact atggggacca
     5461 aatgctgcgt tcaactacac tcgttatgaa tcagaagaaa atacaaaatt gatcaacgat
     5521 atcagttcaa gcaaagcatt tgatgaagag taccaaaaag aagcatatca aaaatggcaa
     5581 caatatgcat ttgaagaagc atttgcgatt ccaacgttgt tccgtaatga agtattgcca
     5641 gtcaacgacc gtgtcaaaga ctgggattgg tcttatgatg taccaaaccc atgggcaaat
     5701 gtttctgtta cagcagattc acgttcataa tcactaaaaa tgaccgaaaa agggcgatgt
     5761 cctttttcgg ttttttgata tttttctgtt tatttctcta atattcaggt caaggcgctg
     5821 atttcatgac ttctccaata aacggtgttc aaagtccagt ttctcggtat taagtcaaat
     5881 ttatgcaaaa atatggaaag ccattttcac aaaatttttt cttcatactc gaaactaaac
     5941 gaatttttca caacttctcg tcaatcacta ggattattaa agctttactt accttttgac
     6001 cgaaccattt caaatttgac aaaaaaccag tataataagg aagtatcgaa aaaatatcca
     6061 taaaaggaat gtggaaaatg gacaaccagc tgacaaaaga actaaaagaa aactataaca
     6121 tcgtttttca cgatctaaat ttgcttgaac aagcttttac ccattcatcc tatgtgaatg
     6181 agcatcgcaa tctgcaatta tcggataatg agcgacttga atttttagga gatgccgttt
     6241 tagaactgat ggtttctcag tatttattcc gtttatatcc tcatttgcca gaaggaaaat
     6301 tgacaaaaac acgagctgcg atcgttcgcg aagacagctt gtcgaaattt gccaaagaat
     6361 gtcactttga tcaatatgtt ttgttgggca agggagaaga gaattcagga ggacgtacgc
     6421 gaccagcatt gctttgcgac ctattcgaag cttttttagg cgcgttatat cttgaccagg
     6481 gattcgacac gacccatgca ttcatcgaaa aagtgatttt tcctaaagtc aaagcaggtg
     6541 ctttttcaca tgagatggat cataaaacaa aactacaaga agtcttgcaa aaatcaggtg
     6601 atgtatcaat cgaataccga ttgatcaatg aagaaggtcc agctcatgaa cgagtatttt
     6661 ggatcgaagt ttatgtagat gatcaattga ttggcactgg acaagggaaa tcgaaaaaat
     6721 tagcagaaca agctgcggcg gaaaatgcat tggcagcact gtaaaagtaa gtgagggaaa
     6781 gctttgtatt tgaaacgaat tgaaatagcc ggttttaaat catttgcaga caagacagtc
     6841 attgattttg aaaatagcgt cacagcagtg gttggaccaa atggcagcgg aaaaagcaat
     6901 attacagagg ctatccgctg ggtattagga gaacaatctg ctaaaagtct gcgaggcgga
     6961 aaaatgccgg atatcatctt tgccggttcc gatacccgta agcagctgaa tattgcagaa
     7021 gtcacagtga ttttagacaa tagtgatcat tatctgccat tggagtacaa tgagatcagt
     7081 gtaacccgcc gttatcgacg gactggggaa agcgagtttt tcatcaataa acaaagctgc
     7141 agattaaaag atatccaaga attgttctta gattctggat taggaaaaga atcgttctca
     7201 atcatttctc aaggaaaagt agaagcgatt ttcagtagta aaccggaaga ccgtcgtggg
     7261 atctttgaag aagcagctgg tgtcttgaaa tacaagcagc ggaaaaagaa agcagaacag
     7321 aagctttttg aaacagaaga caacctcagc cgcgtccaag acatcatcca cgaattggaa
     7381 gaacagttaa ctcctttggc cgcccagagt gaggcagcaa aggagttttt acggctaaaa
     7441 gaaacactga ctcaaaccga tgtctctttg atggttgctg aaatcaagac agcaaaaaaa
     7501 gattgggaca acaaacaagc acagcttgcg aaattcaatc tagaattagg caaactatct
     7561 gagtcgatcc aagaacaaga atccattttg gcgaagcagc gtaaagaaaa tgcgcaagcc
     7621 gatcgtctga tcgaaaaaaa tcagcaagta ttattagatc tttcggaaaa actgaagcag
     7681 acagaaggac aaaaagatgt tttgcaggag cggacaaagc atacacaaaa aagctcgcaa
     7741 gaatatcaaa cttcattagc agaagcacaa aaaaaggtta aacattttga aaagcttcaa
     7801 gaaagcttga tgaaagcagc agcagaaaaa gaaacagaga tccaaaaagc tgaagcaaat
     7861 ctcataaaaa cacagcaaga attagaaaaa taccaaaaat caacgaaaga attattggca
     7921 gaattaagag atcaatatgt tgatttgatg caagaacaag cagctgtagg aaacgaactg
     7981 aagtatctag aacgtcaata tatacaagaa acagcaaaaa gcaagcagac gttagccaag
     8041 caatcggaag tagaagcatc agtagaccga ttgatcttac aaaaagaaga attgacacaa
     8101 aagcaggcgc agctaaaatc atctttaact gaaacaaaag agaagttaga aatgatccaa
     8161 caaaacggga aaaagttcca agaagcatta gcaaaagaac agcctaaaat gtatcaatta
     8221 atgaatcaag tccagcagct acgtgctcgt aagaaaagcc ttcaagaaat ccaagaaaac
     8281 tattttggtt tttaccaagg ggttcgattg gtcttgcagc ataaacagca gctttcgggt
     8341 attgtcggag cagtggctga actgatcgat gttcctgcag atttcacgtt agctatcgaa
     8401 acagctttag gtggggcagc ccagcatgtc atcgttgaaa atgaaaaaga tgctcggcaa
     8461 gccatcacgt atttgaaaca gcaaagagga ggaagagcaa cattccttcc actgacgaca
     8521 atcaaaccaa gacaattacc ggctcatata ttgacacaag cagcagctgt agaagggttt
     8581 atagggattg ccagtgaaca agtcagttat cccgatcaaa tccaaactgt ggttcaaaac
     8641 ctgctaggaa cgatcctttt agccaaagac ttaactagtg ccaatgcaat cgcgcaaacg
     8701 atccgctatc aatatcgagt ggtttcctta gaaggtgatg tgatgaatgc cggaggatcc
     8761 atgacaggtg gagcgaataa acgaggaaac caaggcagtt tgtttgtaca aaatcaagaa
     8821 ttgaaacagt tgactagtga atttgaagaa gcagataaac aactccaagc acaagaaaaa
     8881 aaagttcagg agctgcaaca agaaacagct cgtttagccg aagaacaaga agttttgcgt
     8941 acacgaggcg aacaattacg ttttgaagaa caagaagcga caaatcagct acaaaacatc
     9001 ataaatgaac ttgaacgttt tgaaaaagaa aagcaaatct caacttttga gacacgtgaa
     9061 ctgcagcagt ttatcgaaga ttacgaaaag caaacgaacg aattgaaaga caaacaaaca
     9121 gatcttgaat cgcaacgaca aaaaatcgac gaagaaatca aatcgttaag tcaagaaagc
     9181 gaccagatgg aagccagaag agcacaagta caaagtcaaa aagcacaaga acaagcagat
     9241 cttgctgtct tgaaagagca gtttaatcat ctgcagatcc agctccgcgg tgcccgagtc
     9301 caaaaagcag aggcaaccga acgtcaagaa gcgatagaaa agcagttggc gacattgaca
     9361 gcagactttt ctgatcatga agtcacagaa gaaagtttgg aaaaacaaat taacgaatta
     9421 tctgctcagc gagaaacatt aaaagccgag ttggcaaaag ccaaagagca gcgagataga
     9481 aggcaaaagg agatcgataa gcttgaagca gtcttggcag agcgtaatcg tgagcaaaaa
     9541 gcacgactgt cggaacaatc caaattagaa gtccaaaaag accgagctga aatgatttta
     9601 gataatcatt tgacctatct gcagtcagaa tatcagctga catttgagaa agcttcccaa
     9661 gactatcaag aaacaaccga tatcgaagac agtagaacaa aggtttcatc cttaaaagaa
     9721 cagattgaaa aactgggacc agtaaactta aattcaatcg aacaatacga acaagtcagt
     9781 gaaagacata cattcttagc aacacagcga gatgatctgt tagcagcgaa aaaccaattg
     9841 tttgaaacga tggatgagat ggatgatgaa gtgcggacaa gattcaaaga agtatttgaa
     9901 gctatccgtc aagaattcaa aattgtcttt ccaaatatgt ttggcggcgg taaagctgaa
     9961 ttagtactga cggaaccaac tgatttgctg aaaacaggta ttgagattga agtacaacct
    10021 cctggcaaaa aactgcaaag tctaagtttg ctttctggag gagaacgagc acttacagcc
    10081 atcgcgttgc tcttttccat tatccgtgtc tgtcctgttc cgttttgtat cttagatgag
    10141 gtagaagcag cgttagatga agccaacgtg aaacgttttg gtcgttactt gagcgatttc
    10201 caagatgaca cacaatttat cgtagtcaca caccgcaaag gaacgatggt cgcagcagat
    10261 gtactgtacg gagtgacgat gcaggaatcc ggtgtatcaa aaatcgtttc tgtacggatg
    10321 gaagatatca atgaagaggc gaaaatcaac gaaacgaaag tgtaggaacg aaaatgatca
    10381 aattagtagc aatcgatttg gacggtacat tattaaatga acaaaagctg atttcagatg
    10441 agaataaaca agcattagca caagcaaaac agcaaggggt caagatcgtt ctatgtacag
    10501 gacgcccctt agctgcgatg gcgcattatc tgcaagaatt aggactggta gatgaaggtg
    10561 actacagcat cactttcaac ggcggtttgg tacaaaaaaa tgatactggt gaaatcatgg
    10621 aaaagaaagt gatggaagta tcagatatcc aacgcttata caaactggct cagcaattgg
    10681 atctgccact agatgttttg tctgacaatg tggttttaca gctgcctagt gccccaagga
    10741 agcaatcctt gtacaatgtg ttgaataagc tgctgcaatt ccaaccagcg gatataagcg
    10801 atattacaga tgaatttgtg cttaataagg cagtaatcgc ttatgaccaa gaagaattag
    10861 atcctaaaat caaagaaata ccagcagaat tccatgaaat gtatgagatc atcaaaacaa
    10921 gaagtatgct tttggaattt atgccaaaag gtgttacaaa agcttatggt atttctttat
    10981 tggcaaaaga tcttggtttg aaacaagaag aaatcatggc gatcggtgat gaggaaaatg
    11041 atttaccgat gatccaatat gctggactag gcgtagcgat ggagaatgct gtgcctttcg
    11101 tcaaagaagc agcagatttc gtgacagcca gcaatgaaga aaatggcgta gccaaagtga
    11161 tccaaaaatt tgtattagag tagggggagt caaatgggat tttttgataa aatcaagcgt
    11221 gcttttactg gcgaagagaa aacagaagaa gttgaagtac aggagaagta cgacaagggt
    11281 ttagaaaaga cgcgtaaaac atttggtgaa cggatgaatg agttgtttgc caattttcga
    11341 acagtcgatg aagacttttt tgaagaatta gaagaaacac ttatcggtgc agatgtaggt
    11401 tttgaaacag cgatacgaat taccgaagca ttacgtcaag aagtgaaact cagaaatgcg
    11461 aaaaaatcgg cagaagtcca aaatgcgatc atcgagaaac ttgtggatct ctatgaagcg
    11521 gaaggaatca atgaagtaaa tgaactaaat atccaagaag acggactaac agttattttg
    11581 tttgtcggtg ttaatggtgt agggaaaaca acaagtattg gtaaacttgc ccatcagttc
    11641 aaaaacgaag gtaaaaaagt attgatggca gcagcagata ccttccgcgc tggagcaatc
    11701 gaccagttag ttgtctgggg cgaacgcgct ggtgttgaag ttgttcgtgg caatgcagga
    11761 ggcgatccag cagctgtcgt attcgatgct ttggaaagag caaagacaga gcaggcagat
    11821 gttttactag ttgatactgc aggccgactg caaaataaag tgaacttgat gaacgaatta
    11881 gaaaaaataa aacgagtaat ccagcgggaa ttaccagaag caccccaaga aacacttctt
    11941 gtattagatg caaccactgg tcaaaacgcg atggttcaag caaaacaatt caaagaaaca
    12001 acagatgtga ccggtcttgt tttgacaaaa ttagatggta cagcaaaggg cggtatcgta
    12061 cttgctatcc gtaatgaatt gcatttacca gtgaaattgg tcggtcttgg tgaaggaatc
    12121 gatgacttgg agccgtttga tccaaatgat tttgtcgttg gattgttcaa aggattacta
    12181 aaagaagaat aacccatgtc taaaaagcag gagctgtgac aaaagttgca gctccttgtt
    12241 tgaatgattg tttttaacat agctgtgttt ttgatgcctt catttttata cttatttgta
    12301 agcggttctc aaacaatttc gttctttttc ttagactttt tgattataat tagattcata
    12361 tgaattggag gtcacttaat gatgaatttt aagcaattat tattgcatgt caatgcgcgc
    12421 ccttttatcg accaagctcg atttggtata gaacgggaag gacagcgggt tgatcttgca
    12481 ggaaatctag caaaaaccga tcatccagca atctttggcg atcgatccta tcacccctat
    12541 atccaaacag attttagcga aacacaaaca gagatgatca cccctgttac cgattctatt
    12601 cccgaattat ttcagtatct cgctgctgtt tatgatgtga ctgctcgttc tataccgaaa
    12661 gaagagatga tctggccatt aagtatgcca cctgccttac cggaaaaaga cgaagagatc
    12721 attattgcaa aattaaaaaa tttcgaagat gtcttgtatc gacgttattt agcaaaagaa
    12781 tacgggaagc gaaaacagat ggtaagcggt attcatttca attttgaatt tggcgatgag
    12841 ttgctaagaa cattgttcag ccatcaggaa gaatttcaag atttttccga attcaaaaca
    12901 gaactttatt tgaaaacagc gagaaacttt atgcgctatc gctggatgat cacttattta
    12961 ttcggtgctt caccaatgag tgagaagaac tactttttag atgaatcaca tccgcaagaa
    13021 cctgttcgca gtattcgcaa cagtgcgctg ggctatacga atcatccaaa tgtgaaagtt
    13081 tcctatgctt ctatgaaaca gtatttagca gatatcgagc gaatgatcga agaagggaaa
    13141 ctttcggagg agaaagaatt ttatacaccg cttcgtttcc gaggcggaaa gaaagtcgca
    13201 gatttagcaa caacaggtgt tcgttatatt gaattgcgga atatcgattt gaatccttat
    13261 gcaagattgg gtatcaatcc agagcaagtt cggttcttac aattattcct gatgtacatg
    13321 ttatggacag aagaaaaaga agactgtgac caatgggtag cagaaggtac gactcgaaat
    13381 aacaaagtag cactagaaca accatcagat caaacagaat ttcatcaaga aggtagagaa
    13441 attcttgaag gaatgaagca aatgctggtt gaattggatt ggttggattc tctttattta
    13501 gtagaggaag cattgactca aatggatcat cctgaacaaa cgttagcagc gaaactctat
    13561 caagaagcac agctatcgag tcagcaagaa gttgctgtgg cattgggaca tcaatattat
    13621 aaagaaagcc atgaacgtcc ctatcagttg gccggttttc gcgaaatgga actttctact
    13681 cagatattta tgttcgatgc catccaaaaa ggtgtccaag tcaaagtatt agatgagtcg
    13741 gatcaatttt tgcgtctgca attccaagac catgtagaat atgtgaaaaa tgcaaacatg
    13801 acaagtaagg acagctatat cgtcccactt atcatggaaa acaaaacagt aacgaaaaaa
    13861 gtcttaaaag aagctgggtt ccgggtacca ggaggcgcag aattttcatc catggaagaa
    13921 gcggtaaaag cttatccgag atttgccgag caggcatttg tgatcaaacc taaatcaacc
    13981 aattacgggt taggtatcac gattttcaag gaaggagcta gtctggaaga ttatcaagca
    14041 ggtttagcca ttgcttttcg tgaagacagt tcggttttag tagaagaatt tatgccggga
    14101 acggaatatc gtttctttgt gatcgacgga gaagtccaag ccatcatgtt gcgagtccct
    14161 gctaatgtta ttggtgacag tatccgaaca gtgaaagaac ttgttgaaga aaaaaatagt
    14221 gaccctttgc gtggaacgaa tcatcgtgca cctttagaat tgatccaatt aggtgaatta
    14281 gaacaattga tgttgaaaga acaagggttg acgatcgaat cagttcccca agccaatcaa
    14341 attgtttatc taagggagaa ctcaaatatc agtacaggtg gtgattcgat cgatatgact
    14401 gatgaatttt ctgaagctta caaaaaaatc gccgtttctg cggtagaagc actaggagct
    14461 aagattagtg gtatcgattt gattatacca gataaagaaa tcgatcctac aactgataaa
    14521 aaagcatatg ggatcatcga agcaaatttc aatccagcca tgcatatgca tgtttatcct
    14581 tttgcaggaa aaggcagaag attgacgatg aatgtattga aattgttata tcctgaagta
    14641 ttttaaaaga aaaagctttt gacagattgt cctatttgat gtcaatctga tcaaaagctt
    14701 tttttgcatg taaaaattta tgtgtttttt cgttttaaaa taaacgatcc cagaagcttt
    14761 ttttcttttt tggtttttct ttttcagctg gcgaagagtt ttcttctggg tatttttttt
    14821 cgatatcaat cacaggttca tttactgctt cttttgctgt taagagcaat ccgaattcct
    14881 caggatttgc agtgacgtaa tcgttgacta cagtgaaacg gctattgttt ttcgttgcta
    14941 gctggatata ttggttttgg acagaaaggg gtagagcccc attcaagagt atggaagcat
    15001 ctggatgttt tttgagttct tttaaaaagt tttctttgtt ttttggttgg atcatttcag
    15061 caacagtcat actaagatag cagcgttccc tgaatgtccc taaatattta tgctgttctt
    15121 cagggttcac taaaggtgta ccataaagtc ccttatccaa atggcttttt acatcatctt
    15181 gtgccataaa aaataccctc cttcttctct ttgtctttct tacgattata gcatattccc
    15241 aataatacct aaaacatatg aaaaagcggt tgcttttgtt caacgattca cttaaaataa
    15301 gaatgaaaat cattctcaat tagaaaggtg gattggatgc gctacggacg actagtagca
    15361 ttattagtta ttttaagtag tatttcattg ttcatcggag tatcgaatgt acaattgtca
    15421 gaactgctcc agttagatgg gcaaagctta caaatcttgc tagtgactcg tttgccacga
    15481 acgatttgtt tgatccttgt gggagcgacc agtagtattt gtgggttgat catgcaacat
    15541 ttgacccaaa ataaatttgt ttcaccaacg actgcgggca cgatggatag tgcgcgttta
    15601 ggtattttag tggctatgat tttcctacct ggtgcatcct tgattgtccg atccttgaca
    15661 gcattttgct ttgcttttgc aggaacattg ctttttttat cccttacgcg attgttcctc
    15721 cagaaaaatc aattgatgct tccgttgatt ggagtcatgt tagggaatat cattggttca
    15781 gttgctactt tttttgctta tcaattccag ttggtacaaa atatgacttc atggcttcaa
    15841 gggaattttg cgactgtgat acgaggaaat tatgagttat tgtatgctgt tgttccattg
    15901 ttgctcttgg tttggctatt tgcttaccga tttacgatca ttggtctggg cgaatcgttt
    15961 gcagtaaatc tgggaataga ttaccagaag atgcagtttt taggcattgg cttagtatca
    16021 ttggctagtg cagtcatgtt gatcatggta ggaagcattc cttttatcgg agtgatcatt
    16081 ccgaatcttg tctctagaat atatggagat caagtgcaca aaacaattgg cgtaactgct
    16141 ttagcaggta gtttgttctt agtattttgc gatcttgtcg ctcgcgtgtt gatttttcct
    16201 tatgaaatac cagtcagtgt gatcgttgga atcatcggtg gggctatctt cctttatctt
    16261 ctgataaggg gacgacgata tgcttaaaaa aaagccatct atttttattg gtatgcttgt
    16321 tcttgtttta tttgttacag gaatgtactt gacgatccat acatatggaa actggcattt
    16381 tgcatgtgca ttgaggagta agaaaatttt tgcatttatc cttgtctcgc tagctgtcag
    16441 cagtgccaca atcagctttc agacaattac gcataaccag tttttgactc cttcagtatt
    16501 gggacttgag tcgttatatg tccttgtcca aacggtcctt ttcttcttta tcggaggagt
    16561 gacgatgctt cagcagacag gtacagtttt tttcttattg aacgttgggc tgatggtcgg
    16621 gctgagtcta ttgttatttt tctttctttt gaaaaaaatg agagcgaatc tttttttatt
    16681 actgatgtca gggatgattt taggtacgct ttttggtagt gtcagtacct ttttacaagt
    16741 catcatggat ccgaatgaat atgatttgct tcaaggcaga ttatttgcaa gctttggcaa
    16801 tatccagagt cagtatcttt tgcctgcttc tttgatcatt atcggctgct ggtacctgtt
    16861 atttcgatat cgacatgtgc tagacgtttt ccatctcgga aatgaacaag cagtaaacct
    16921 cgggatagac acagatcgtt ttgaattttt gattttatgt tgtgtcagtc ttttgacagg
    16981 gacatcaacg gctcttgtgg gtccagttac ttttttaggg tttattgtcg caaatatcag
    17041 ttatcgtgtc ttttctactt atcgtcatgg tcagctattt atcggaagct ttctggtagc
    17101 tatgctgttt ctgacaggag gacagctcgc agtagagcag attttcggct ggaatacaac
    17161 agtgagtgtc gtcgttgaat ttgtcggagg aatttatttt gtcacaaaat tgattcgcga
    17221 aaggaagagt atcctatgat tgatttacaa aaggtcaata aactgtacca tggaaaatca
    17281 gtggttaaag atgtacagtt aacgattcct gaaaagaaat tgactgcttt tatcggtcca
    17341 aatggtgctg gaaagtcaac ggtgttgtcg atgatcagtc gtttgatacc aaaagataca
    17401 ggtgaaatct atttagacca taacgaagta aaggcttgga aatccaatga attagccaaa
    17461 aaattatcga tcctacgaca atcgaatgaa ataaatgtcc aaatcaccgt gaaagaatta
    17521 gtagaatttg gacgatttcc ttatacgaaa ggaaaattga cgacaaaaga cgaggaaatc
    17581 gttcaggaat cactggaaca ccttgggtta gaagaaatag cggatcaatc gattgacact
    17641 ctttcaggcg gtcaactcca acgtgcgtat attgcaatgg tgctagcgca agacacggat
    17701 tatattttac tggatgaacc tttgaacaat ctagatatga attttgcggt tcaaatcatg
    17761 cagattttaa aaaatttagt acaagaaatc gggaaaacaa ttgttattgt tcttcatgat
    17821 atcaactttg ctgcaagtta tgcagacgaa attgttgcaa tgaaagatgg acagttattt
    17881 acgcaaggaa cgacccaaga aatgataaaa aaagatgtat tggatgaatt atacgaaatg
    17941 gacatacgga tttgtgagat tgaaggccgc agattctgtc tttatttcta aaactaacat
    18001 taggagagaa gagatgaaaa aattagtagt aggtatcttg attctagcat catttggttt
    18061 agcagcatgt ggaaatagca acaacaccag tcaggcagac acaaaaaaaa gcagcacaca
    18121 aactgaaact acattgacga taacagatag taatggagac caaatcgagg tgccaaataa
    18181 tccagaaaaa gtcgtcgtat tcgacaatgg ttctttagat acgatggatg ctcttggtgt
    18241 tggagataag gtagttggag cagctacaag cagccttcct gagtatcttt catcctataa
    18301 aaaagtagaa tcagcaggtg gaataaaaga accagactta gaaaaaatta atcagcttca
    18361 gccagatcta atcatcattt ctggccgaca aagagatttt caaaaagatc taagtcaaat
    18421 tgctccgacc attttccttt ctttagatgc gaaaaatcca tgggaatctt tccagcaaaa
    18481 tgtcactgct ttaggggaga tctttggtaa gcaagaagaa gctaaaactc aattagagga
    18541 attatctagt gcaatcgatc agacgaaaaa gaaagcagaa gctactgaca aaaaagcact
    18601 agtcacactt gtgaatgaag ggcaattatc agcctacgga tctggttcta gattcggttt
    18661 catccatgat ttatttggct ttgaacaagc agatgatcaa atcgaagcat ccactcacgg
    18721 tcaaagtgtt tcttatgaat atgtgctgga aaaaaatccg gatatcttat ttgtagtaga
    18781 cagaacaaaa gctattggtg gagacgacag caaagatgat atttctgcca atgaactggt
    18841 agcacaaacg aatgctggta aaaatcagca gatcatctcc cttgaaccag atgtctggta
    18901 tttaagcggg ggcggtttag aatcaatgaa actgatgatc gaagatgtga atcaagcatt
    18961 taaatagcat aaataacaga ggattaaact ctcatactat gtaacaataa tgtttcttac
    19021 attcttaaat agcaagagcc tatcctcaaa gaagttatca aaaaccatcc gtaaaatcgt
    19081 attgatgatt ttacggatgg tttttattta tttgagaaat cttttttcaa cgtgaattca
    19141 ttatctacat catacgggtt gattctagaa tggggagtgg caaaaaatag tgtgatccat
    19201 tttttcagca tcaagatcgc ctcctttgat ttgaatcaaa ggtacaaagc gaaacttaga
    19261 taaaacttaa atttagatat ttttattttg taccgatctc ttctttaaac gatgttccat
    19321 ctgctgcttc tcggcaataa gtccactaga ctagaaaata tgagaagcta ttttctgtcc
    19381 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa cgatgttcca
    19441 tctgctgctt ctcggcaata agcccactag actagaaaat atgagaagct attttctgtc
    19501 cagcgttctt attactcaaa gcaaatcgca gttgtcacaa tctcttctaa acaatgttca
    19561 atcagctgac cttcggtatt agctcaaagc actgatttca caacctctat ttaggcatgt
    19621 gtctcagcaa gcggttgata gttgatgatc tgcatttttc gataaaaatc ggcgggaagc
    19681 ttactccacc gttttaataa ttgatagaaa taactctctt ctgctttgat tttatactcg
    19741 cttaatatca atccaaaact attcgttttt tttgaagcta attttttata aatatccaat
    19801 gctttggttt tatcttcatt gacaaagagt gtttttactt caatataaga accctcgata
    19861 ttttgtgttt cttcgataaa atagatgttt tttatatatt ccttcatagt gattcctcca
    19921 aagaagtctt ttatttgata agtttactct accgaagagt tgttaataat ccgtgaacaa
    19981 aaagtaattc tttttccaat agttgttaaa ggttttttat tttttatagt attttttgtt
    20041 atttaagaaa ggaataaggt taaacaaata acaggttgct ttttgggtct ttttccgttc
    20101 aagaaactga acgaagaaaa gggaaagttg tctcttttgt gataagagaa atcataataa
    20161 acgaatgact tactttccaa taaattgaga acagtcagaa ccagacaaat tagttgcatc
    20221 tgatgaataa agtgattatt ctatgacatg aggtgataca gatgaaatta gaagtgacaa
    20281 aagaagcaca agaagtattg aagaataaat tagaagcaga cgatcaactt ttattagata
    20341 tcgaaaacgg tgacggtcca tttgcagaaa gccaagtcag ttgccaattg gatacggcat
    20401 ttcgattgat cattgtcaaa aaagatacac aaacagatct aagtctttat tctgtagtgg
    20461 ctgatacacc agttggacca atcaaaatca aagacagtgc aatactttac atggatgatc
    20521 catccacact tgctttagaa ccaacttata atagtttaca attaaaagga ccaagcggct
    20581 tgagaaaagg caacttgcaa gttgttcgaa aagactagag ggataaaatg ggaaaattgg
    20641 cgatcatcag cgacctacat gtggacatca ataaattcgg cgaatcagag ctgatgcagc
    20701 tttggcaagt tttgcaaaag cacaagatca ctcggcttca tctagccgga gatacggcta
    20761 ataaagtaga ccgttgccta gctgttgtcg aatttttcgt caataaagga ttatctacga
    20821 catttcattt cggcaatcac gagttggcag atgtgcccaa tgaacagatg atggaccatt
    20881 ttccagaccc acatttttta aatgaacggt atattgcttt aaatgagcag accgttcttt
    20941 tagggatgaa tggctggtac gactatcagt tttccgaatt gaaagattac gaggcaatcg
    21001 tacgaatgaa gcgtttgtat tggtatgatc ggttcatcca gcgtgggaaa acagatctcg
    21061 aggtcaatgc agaaatgctt gcagctgcaa aaaatttgct agaccagctg gaagcaaaaa
    21121 aacttgaggt gattttatcc actcatttcg ttcctaaacg ggcatttatc atgtatcaat
    21181 ctgctcctta tgagcgttgg aacaaattaa atgcattttt aggatcagaa tcattcggag
    21241 aattacttga ccgttattca aatatcaaac aagtagtttt tggccatacg catcgtcgct
    21301 ttgaagataa atccatcggt cacacgattt acagctgcag accttttggt tattattatg
    21361 aatggcagct gacccgagat tttgtcttga aagaacagtt gctagacagc tatgatccta
    21421 tgaaattacg tgtcctcttg cgtgcccatc acccagcttt tcaggcctat caaacacaac
    21481 atttgcaaga ggaatttgaa caagcgatga caatcgttcc gtattgagac agcattagta
    21541 atgtgtaaaa gaaaatacct tgtaaaacat aaggataagt aagagcagta gatccattta
    21601 tcgtggatat gctgcttttt ttgcggaaaa taatcagcga atgtttattt gatcggatga
    21661 tttgtaactc gcagagtact agatgcaagc gtgtattctt tttttgaacg ataatcacaa
    21721 aaaaaaatat aatcgtaaat ttcaagttta agttagccac ccatggtgac ccggttgctc
    21781 gttaccacca accgtctaat ttttctaatc gagtgggccc ttgacaatga gctgaggcat
    21841 ggtagtctcc cgactggctg gccccggcca gctttccaga cacaagccat gccgagggag
    21901 gctcgttgtc aagggcccac gtgtcaagcc gtcaaccgtt cctgcctaaa tgcctggacg
    21961 aacgtgtcat gcggcgttgc atagcccaaa atctttcgcg ggtaatcgtt catccaacgc
    22021 tgaatccgca agatttggtt ttcgctcacg ttgccgattg ggctgccctt ggggatgaaa
    22081 cggcgaatca gcttgtgttg gttttcgctc gtcccgcgct cccaggaagc ataggggtgg
    22141 ctgaagtaga tgtttgccac atcctgcaag gcatcgtgca gtcccgcgaa ctccgagccg
    22201 ttgtccgagg taaccgtctt gaacagctgg ccgaagtacg caccagcccg ttctctcagc
    22261 tgtcggaccg cttggttgac cgattcggca tccttcccgg ccagcttcag aatgacctcg
    22321 aagcgtgtct gccgttccac caacgtcagc agcacggcat ccgacttggt cttgtttcct
    22381 acgaccgtgt cgacttccca atggccgaag gttttccggg taccaaccgc ctccgggcgc
    22441 tcttcaatgg atttccctag cacccgttta ttcgggcggt tgccatgacg ggacgatttc
    22501 ggtttgcggg acagtttttc tagcaggtcc atattcttcg ttttcatgat gccacggtcg
    22561 atccactgat agagggtggt ggtcccaggg ataatggccg ggtcgaacaa ttcgtgtttt
    22621 ctggcaaagc ccaccaccac gtccggggac catttttcca acagcatcat gtcgtccgcc
    22681 cattcaatga aggcgtccgt gtcggcccat aagggccgtc tgccgcagtt caagcggttc
    22741 ttctcgtagg ccgcctgccc ggcatcggcg tcgtagacgt catgataata gtcgtagacc
    22801 ttcccgttct gattctggcg gttaagctga gtaacggtcc cgcgtttgat ttcgttgttt
    22861 atggtctgcg gcacgcagcc caggacatcc gcaacctgac ggttggaata gttttccttt
    22921 ttcaggatgg cgatttggca acgttctgag taggttaggt gttttccctt gcgtgaaact
    22981 gtgttatcat ggttttgcgt catgtgaatt catccttgtc tattgagagt tgtggtaact
    23041 tcaatataac atggaattca cgtggcgttt ttttatttcc aaatggctaa gttaattata
    23101 aaatccgcca aaaaaatata atatacattt tttatttaat tataattaat attgattgaa
    23161 gtctaggcag tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag
    23221 gctatgacag ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc
    23281 aaatcgggct gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt
    23341 tcttttattt cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga
    23401 tatgtttagt aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag
    23461 cagcaatgat gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat
    23521 acccaaagga atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa
    23581 ctcctcctgt tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc
    23641 caagccctgg aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga
    23701 ctcaaaaaat ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg
    23761 ggtctagaaa accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga
    23821 aattaagtgt ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag
    23881 cacagctgaa atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc
    23941 aaacggtgaa ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg
    24001 ctaaatcagg aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa
    24061 atcaagatgc ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt
    24121 ccactaaatt agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact
    24181 gttaaaaata aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag
    24241 gctaggctaa atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa
    24301 aagatatatt gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata
    24361 tctttccgat catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag
    24421 acgaagaaag caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag
    24481 aaagcagtga ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg
    24541 agggttcgga caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg
    24601 atcccaatat tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca
    24661 tattggaaaa tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta
    24721 tttggtcaaa taagaagatt agtttactct cggattttac atttaaatct tatttatact
    24781 taggaaatca acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta
    24841 gaatggctag taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt
    24901 ctactcgttc tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata
    24961 aaaatagtgg ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg
    25021 gacatgtcgc ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg
    25081 gcgtgacggt agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat
    25141 gggacgcgga gcaaagaagt cttacttatg atttggaagg tgtcggtcac tcttctcgga
    25201 ttatacctga tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt
    25261 caacaggtac tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca
    25321 catcaacagc tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag
    25381 gaaacgataa gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata
    25441 agaaaatgca gactagtatc gacctcccac aagaattaac tattcccgag catatcttga
    25501 tcaatggaaa tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa
    25561 atctgacaga ttattttatt gctggagata cgttaataat cgaattggag acaactttgc
    25621 aggaagtaat tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt
    25681 tgattgagca atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc
    25741 atgtttatta taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg
    25801 ggaaaatagg agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact
    25861 cggattcagc gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt
    25921 acagaaaagg caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata
    25981 aaatatccag tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag
    26041 atgaacgggc ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg
    26101 gagattttga gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag
    26161 ggacatccgc tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc
    26221 ttttagattc tacagctcat cgtggaatca gaatagatat tcctgtagaa aaccaaagaa
    26281 tcggaacctt tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg
    26341 aagagaagaa tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt
    26401 gtgtcccaca gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat
    26461 tttcttgaga ccaaaacaac aattatttat aacactccta gttattttag gggcactttt
    26521 taacaatata tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga
    26581 gaatcaaatc gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt
    26641 agaagtaaaa gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt
    26701 gaatagcgca accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa
    26761 agacatgagc ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct
    26821 aaaacctaaa agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga
    26881 tggtgtattg gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga
    26941 aactaaggat caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat
    27001 gcaacagaca ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca
    27061 aatcaatgca agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa
    27121 tcaagtagtt attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga
    27181 ggaaaaagaa ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa
    27241 tggtcaaccg ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa
    27301 tgaaaatgga aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg
    27361 ggtatttgaa aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga
    27421 tgttacgatt aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt
    27481 gtttatttta ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta
    27541 aggttaagat aacaaatagt aaatttttat tattgtagga tggtt
//
LOCUS       DACDTO010000028        27332 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDTO010000028 DACDTO010000000
VERSION     DACDTO010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.028"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            203..3730
                     /locus_tag="IWK70_001258"
     CDS             203..3730
                     /locus_tag="IWK70_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9613822.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3727..7449
                     /gene="addA"
                     /locus_tag="IWK70_001259"
     CDS             3727..7449
                     /gene="addA"
                     /locus_tag="IWK70_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9613823.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7518..7859
                     /locus_tag="IWK70_001260"
     CDS             7518..7859
                     /locus_tag="IWK70_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9613824.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8168..9211
                     /gene="pheS"
                     /locus_tag="IWK70_001261"
     CDS             8168..9211
                     /gene="pheS"
                     /locus_tag="IWK70_001261"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9613825.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9216..11636
                     /locus_tag="IWK70_001262"
     CDS             9216..11636
                     /locus_tag="IWK70_001262"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9613826.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11806..12816)
                     /gene="trpS"
                     /locus_tag="IWK70_001263"
     CDS             complement(11806..12816)
                     /gene="trpS"
                     /locus_tag="IWK70_001263"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9613827.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13070..13807
                     /locus_tag="IWK70_001264"
     CDS             13070..13807
                     /locus_tag="IWK70_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9613828.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13821..14636
                     /locus_tag="IWK70_001265"
     CDS             13821..14636
                     /locus_tag="IWK70_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9613829.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14649..15296
                     /locus_tag="IWK70_001266"
     CDS             14649..15296
                     /locus_tag="IWK70_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9613830.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15312..15968
                     /locus_tag="IWK70_001267"
     CDS             15312..15968
                     /locus_tag="IWK70_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9613831.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16130..16951
                     /gene="racE"
                     /locus_tag="IWK70_001268"
     CDS             16130..16951
                     /gene="racE"
                     /locus_tag="IWK70_001268"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9613832.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16954..18309
                     /gene="rph"
                     /locus_tag="IWK70_001269"
     CDS             16954..18309
                     /gene="rph"
                     /locus_tag="IWK70_001269"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9613833.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18310..18828
                     /locus_tag="IWK70_001270"
     CDS             18310..18828
                     /locus_tag="IWK70_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9613834.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18935..19435
                     /locus_tag="IWK70_001271"
     CDS             18935..19435
                     /locus_tag="IWK70_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9613835.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19715..20635)
                     /locus_tag="IWK70_001272"
     CDS             complement(19715..20635)
                     /locus_tag="IWK70_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9613836.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20800..21813
                     /locus_tag="IWK70_001273"
     CDS             20800..21813
                     /locus_tag="IWK70_001273"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9613837.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21923..22678)
                     /gene="fabI"
                     /locus_tag="IWK70_001274"
     CDS             complement(21923..22678)
                     /gene="fabI"
                     /locus_tag="IWK70_001274"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9613838.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22898..23338
                     /gene="fabZ"
                     /locus_tag="IWK70_001275"
     CDS             22898..23338
                     /gene="fabZ"
                     /locus_tag="IWK70_001275"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9613839.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23453..24214)
                     /locus_tag="IWK70_001276"
     CDS             complement(23453..24214)
                     /locus_tag="IWK70_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9613840.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24403..25497
                     /locus_tag="IWK70_001277"
     CDS             24403..25497
                     /locus_tag="IWK70_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9613841.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25583..26542)
                     /locus_tag="IWK70_001278"
     CDS             complement(25583..26542)
                     /locus_tag="IWK70_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP9613842.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            27104..>27332
                     /locus_tag="IWK70_001279"
     rRNA            27104..>27332
                     /locus_tag="IWK70_001279"
                     /product="16S ribosomal RNA"
BASE COUNT         9033 a         4748 c         5726 g         7825 t
ORIGIN      
        1 taatacctaa aatgaaaatg gggctgaaga atcaattctg attcatcagt cccataaatt
       61 atagagccgt tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat
      121 aatttgaaga atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat
      181 catgtgttga aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga
      241 tcaccgtcaa gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca
      301 agtatttttc cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga
      361 gatgcgaaga agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt
      421 ttatcgtctg gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc
      481 agaatcagga tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac
      541 aatctttcgc ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag
      601 tgaattgcgt acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc
      661 taatgcagaa gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga
      721 cagcgaattg actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta
      781 tatgcagaca caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa
      841 tggtcaagag tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt
      901 attgctggat cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac
      961 tggaaagctt tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt
     1021 ggaccgattt gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg
     1081 ggaagagaca tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct
     1141 ccatttgtgg gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag
     1201 acgactagtc agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat
     1261 gtctttatat ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat
     1321 cgatgaagaa caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc
     1381 tcttgaccgc tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt
     1441 tctgtcgacg gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag
     1501 gaaaaaactg gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg
     1561 gactcgtgaa aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga
     1621 cgtacagtat ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc
     1681 agtatttttg aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta
     1741 tcaatttttg atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt
     1801 agacagagga aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga
     1861 tctgttggat gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca
     1921 agaaatcatt acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga
     1981 ccaagtgcga atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc
     2041 attagggttg aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag
     2101 tgaagaacga gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc
     2161 aaaggaaagt atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga
     2221 tcgattatat cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc
     2281 ttatttgcgt agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac
     2341 gatcgaaagc gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa
     2401 taggatttat cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct
     2461 caaagaatta gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca
     2521 ggaacattta aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa
     2581 agagatttat acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt
     2641 catgcaattt ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac
     2701 tggtgatttt tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt
     2761 atccttagtg aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga
     2821 agtattcgga gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg
     2881 ctaccagtta ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa
     2941 tacaggcatg catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg
     3001 tatcacaggt ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat
     3061 cgatcgaatc gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa
     3121 atctagcgga cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt
     3181 gacgtatctg gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa
     3241 accagcagga tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt
     3301 agagaagaat acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct
     3361 tttagaagta ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa
     3421 aaaaaatgcg aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga
     3481 agaagaactg caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc
     3541 gattacttcc ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg
     3601 cgaattttgt ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta
     3661 ccaccgtctg gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga
     3721 agaagagtga atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg
     3781 caagcgattt ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa
     3841 actacagtgc ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa
     3901 ttattgattg tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag
     3961 gcattacaag aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag
     4021 cagttagttt tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt
     4081 attcgacgtt attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa
     4141 acagaaacga tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca
     4201 gaaaatgatg aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac
     4261 ggcttgacga atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa
     4321 aaatggctag aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga
     4381 ttatatcaaa aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta
     4441 tatgaacaga tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta
     4501 gctggagaac aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa
     4561 gaagcttatg ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca
     4621 gaattgaaag aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt
     4681 cagcagataa gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac
     4741 aaagcactac cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc
     4801 agtatgcgta aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg
     4861 cagattctca cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat
     4921 cgaaaaaaat tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa
     4981 gccattttat actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg
     5041 gatgtcaaac aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa
     5101 tacgagaatt tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc
     5161 tctagaaaag aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca
     5221 gtgggtcaaa tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca
     5281 gagaatgaac agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag
     5341 attgagattc cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca
     5401 ggactaaaaa ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa
     5461 aaaagtcgtc cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac
     5521 ttaacgattt tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa
     5581 aactattttc aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat
     5641 ccctatcaag atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa
     5701 ccagaattag ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt
     5761 gcttatcaag catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa
     5821 ctagagcatt ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc
     5881 tattatgaga cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa
     5941 gccaatcttt atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga
     6001 ttataccagt tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa
     6061 ccagtcattt caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc
     6121 ttggaattcc cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg
     6181 cgcaatcggt atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa
     6241 acacgagtgt tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa
     6301 ttgttgtcgg aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt
     6361 ttcattgtcg gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct
     6421 gatcatgagg agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg
     6481 aattggattg gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa
     6541 atttcgactt ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag
     6601 caatcgatca ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag
     6661 cagatgaaag aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa
     6721 tatccttatc aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga
     6781 ttattcgaag atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac
     6841 aaagcagcat cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg
     6901 caaaaagatc ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa
     6961 ttactgccat tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta
     7021 aggaaacggt tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg
     7081 ttctttcaaa cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa
     7141 caaccttttt ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa
     7201 gatgaactat taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat
     7261 atctatgatt ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca
     7321 atcgtccaaa aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg
     7381 aataagccag tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt
     7441 aacaaataat tataattgag caaacttgac ttatgttttg taaggattat gctatactct
     7501 gggaagaggt gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg
     7561 aaaaaacatt ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg
     7621 aaaacggtcc gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag
     7681 tattagtcga acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct
     7741 gtagcgaaaa gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag
     7801 cgatgcgtca aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat
     7861 tttgtaaaaa aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa
     7921 aagagtagtt tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt
     7981 gtttgaaaaa tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg
     8041 ttaacgaagt aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag
     8101 acctcgttcc ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa
     8161 caagcacatg tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat
     8221 cacagcggca caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa
     8281 aggaccgatc acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa
     8341 agtcggaagt ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa
     8401 acaagtatta gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac
     8461 actacctggt agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga
     8521 aattgaagat atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc
     8581 tgatcattac aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca
     8641 agatactttc tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc
     8701 acggacaatg gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa
     8761 agtcttccgc agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg
     8821 gctagttgtt gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat
     8881 gaaaaaaatg tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac
     8941 ggaaccatca gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt
     9001 atgtaaatac actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt
     9061 gaagatgtca ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga
     9121 ccgcgtagct atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt
     9181 acgtttctta agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca
     9241 atcgttatgt agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga
     9301 caggaatcga agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg
     9361 gtgaagtcaa ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg
     9421 atattggcga agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa
     9481 tcaaagtgat tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag
     9541 gaaaaatgcg tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt
     9601 cagatagtgt tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg
     9661 taaatggcga tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta
     9721 tcacaccgaa tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa
     9781 tttatcgtca gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta
     9841 gcgataaaat tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa
     9901 tcattgaagg agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg
     9961 aaggcattcg tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg
    10021 gacaaccact acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc
    10081 gtgcaaaaga aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg
    10141 acaatctcgt cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt
    10201 tagactcaga gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc
    10261 caatttcgat tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt
    10321 ttgaaaaagg aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga
    10381 tcgtttctct tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag
    10441 cagaagacgt atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc
    10501 ttgtcgttaa agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg
    10561 agtatgttta ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata
    10621 ttatcgaaga agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg
    10681 gagaaacggt tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa
    10741 tctttgaagg aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat
    10801 caagacagtt tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag
    10861 aacgttctgt attacgaatg aatttgatca gcggactatt agatgatatc agttataaca
    10921 ctgcccgaaa aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa
    10981 atgatccacg tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta
    11041 tttgggaaga aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg
    11101 gtcttttaga aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag
    11161 tgacagaaat gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat
    11221 ttattggttt tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa
    11281 catatgttgc tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt
    11341 tcgaagcagt gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa
    11401 ctgtgacaaa tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag
    11461 atatccaatt atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg
    11521 cgtatagcct gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag
    11581 caatggaaaa agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag
    11641 gaaaatatag taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg
    11701 tgacacaact tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag
    11761 ataaaaaaac aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt
    11821 gattcccatt gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc
    11881 actaccttta cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg
    11941 cgcacggatc ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac
    12001 atcaccaagt ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt
    12061 tccgaataca tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac
    12121 atggatgtgg tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg
    12181 atcggagata taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc
    12241 aggtaatcta cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc
    12301 atatgtatga ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc
    12361 ctcaccaaca ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg
    12421 atagataaag aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt
    12481 gacagtcgga ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa
    12541 ttcagctaat tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc
    12601 cacggctaaa tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt
    12661 agcattatct gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc
    12721 tgcctgcata gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc
    12781 agttggtcga tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt
    12841 aagtctgagc acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa
    12901 aaaacaagga aagaggataa aataactaat agattagaaa tggaagaaat atttatggga
    12961 gaataaacgt tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt
    13021 atacatcttt aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat
    13081 taaatttgat cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct
    13141 cgaattcgag aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac
    13201 gatgcttcgc tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga
    13261 tactgacttg catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt
    13321 tttccaacat ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc
    13381 aatcaaagtg ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa
    13441 aactgtcaat atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca
    13501 gcaacgggtg gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga
    13561 acctacttct gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt
    13621 ggctcatgac ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt
    13681 ggcggatcgt gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag
    13741 agactttttc gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa
    13801 tcattgatag gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag
    13861 gcattgcttt tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt
    13921 tagagcgaag caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat
    13981 ttggaatgat ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag
    14041 caatcactaa aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta
    14101 aaactcggat tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga
    14161 tcacagatga acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag
    14221 ctttgttggt aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa
    14281 cggttctagc agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg
    14341 caaaaatttt agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg
    14401 gagacgcgat gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt
    14461 atgagttagt tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc
    14521 aagaaaactt cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat
    14581 acgataaaat ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg
    14641 gagaggcaat gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga
    14701 tcacattgtt ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg
    14761 caatcttcca attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat
    14821 tttttagaaa cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt
    14881 attggtttaa tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg
    14941 cttttattgc tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag
    15001 caggactatc ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag
    15061 cattcaaaat cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat
    15121 ctattttagc aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg
    15181 aaacattcaa taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc
    15241 taccgttatc ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg
    15301 aggggaaata catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg
    15361 aaggattaaa ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg
    15421 gtggtctgac aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg
    15481 tagtgattga tattatacgg aatcttccat tattattgat cattttcttc acttactttg
    15541 ccttacctca aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga
    15601 tttttgagtc agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag
    15661 gacagatgga agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg
    15721 tgttaccgca agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga
    15781 tcaaagatac ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta
    15841 ttatttatgg acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt
    15901 acttcgtggt atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca
    15961 gttattaata gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct
    16021 ctttccattc gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt
    16081 cacgctaaaa agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt
    16141 gacagataat cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa
    16201 agaagccctg aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg
    16261 cccatatggc cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta
    16321 tctggtggag caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc
    16381 tttagaagaa atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac
    16441 tagagcggca gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac
    16501 ggtaaaaagt caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac
    16561 aagtcttgct tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt
    16621 ggcgaaaaaa attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt
    16681 gattttggga tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga
    16741 aaatgttcaa ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga
    16801 ttatttcaat ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac
    16861 tggctctgcc aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa
    16921 tgtagaacat atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa
    16981 gagcttcgca aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg
    17041 atccgttttg gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct
    17101 tttttgagag aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca
    17161 acgagtacga gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa
    17221 atccagcgtc ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa
    17281 cgtagtatca tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt
    17341 atcactgggg catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa
    17401 ttgtcagaag atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat
    17461 gggacttgcg tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat
    17521 cttgtaatga cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg
    17581 tttgatggtg aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg
    17641 atcaaagagc aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa
    17701 gaaacaaaaa caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat
    17761 atgttcaaag aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat
    17821 gtggaagaaa ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca
    17881 caattgcttg atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc
    17941 ggtatgccag gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat
    18001 aacgccaaat tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc
    18061 cattgtacgt tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg
    18121 cccggtcgag tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt
    18181 atcccagaag gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc
    18241 agtcatcggg ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga
    18301 gagagataga tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt
    18361 gacttagtag atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa
    18421 ttagaagctt ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat
    18481 ggaccaggat tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact
    18541 cacggtcatt tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct
    18601 gcaactgccg atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag
    18661 aaaatcctgt ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca
    18721 tcatatgctc ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc
    18781 catcaggcag ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc
    18841 taatttttaa taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag
    18901 tagtgttgaa tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat
    18961 gctgctggaa aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta
    19021 tcatcatcca ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc
    19081 tgtattagga aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa
    19141 aatgattgac ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt
    19201 gacagaaaca gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt
    19261 gttagtcgat tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat
    19321 tacaagaaaa gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa
    19381 tatattgtct ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac
    19441 gagaaactaa aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta
    19501 tcttgaaaaa gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga
    19561 aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc
    19621 cgccgtttat acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc
    19681 tttttagtga gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt
    19741 tcggaatcgt gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt
    19801 ttaaggcata ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt
    19861 ttccaagatc cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt
    19921 cagctaatgt acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag
    19981 gaacaatccg aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg
    20041 tgatgtttcg attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac
    20101 ggataccaac agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc
    20161 caacatccat ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc
    20221 cttgggcacc aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga
    20281 tcggcacacc gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa
    20341 aaatagtatt taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt
    20401 tagaatactg attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa
    20461 aaaggacgat caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt
    20521 cccaatcaat cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg
    20581 tcaatgtctc gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat
    20641 cctcttttct aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat
    20701 agcaaaaata actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata
    20761 atgataaaat gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat
    20821 ctaggttttt cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa
    20881 tgggaagaag gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg
    20941 cttcattatg gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag
    21001 gtcaatctct ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg
    21061 ctgatgccaa tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca
    21121 aatgaagatt ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg
    21181 attggggtcg gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc
    21241 tgtacgccag taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct
    21301 gattacgatc gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca
    21361 gcgagtttac ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt
    21421 gatccagcca cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca
    21481 aaagacggaa aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat
    21541 tccttgttgc agttagctga agaacgacta ggactgcaag caattgaagg agatattttc
    21601 attgatgatc tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct
    21661 ccaatcggtg ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta
    21721 ggaccagtaa caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa
    21781 gcaccagaag gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg
    21841 taaaaagaaa aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta
    21901 ctttaaagaa tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat
    21961 caccgatgat tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt
    22021 cgatcgtcac accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat
    22081 agtctttaac acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc
    22141 gaattccttt aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt
    22201 tcgcaatacc catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc
    22261 taacgatccc gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag
    22321 agtagctgct gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc
    22381 cggacaattc ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag
    22441 catattcacc gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt
    22501 ctacagtgaa tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt
    22561 ggtaagtata gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa
    22621 tgcttttttt gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa
    22681 tgactctcct ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct
    22741 aaaataactt cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt
    22801 tcttaaatta aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta
    22861 atgaaaatgg caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac
    22921 tgaagtaatg gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac
    22981 tttagaacca ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt
    23041 ccaaggtcac tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc
    23101 acaagcaggt tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat
    23161 cggtgggatc aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg
    23221 tcattttgaa atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt
    23281 agatgataaa aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact
    23341 ataaaacctg atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac
    23401 gcctgaatcc tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt
    23461 gttttgcttc ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg
    23521 aactatctgt ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc
    23581 cgaagacagt ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga
    23641 ttgcttgagg atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga
    23701 taaagaactg gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc
    23761 ttatattata aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc
    23821 ctccagtgcc atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat
    23881 ggaaagtcag tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt
    23941 ttggtgcgtg ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga
    24001 ccagatcttc attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat
    24061 cgacgctact ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg
    24121 aactagaaga agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta
    24181 aactaatact taaaataact ttatttttct tcataactgt tcctttccaa tttactattt
    24241 tgttatcttt aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat
    24301 catttatttt aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt
    24361 aagtggatac aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc
    24421 tcgatttatc cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat
    24481 aagtatgggt ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa
    24541 gttctttcag aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg
    24601 gttgatatca atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt
    24661 ttccataaga tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa
    24721 gcaaaaatga cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg
    24781 aattacgttg acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat
    24841 aatttttatc ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa
    24901 aaattcagaa aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt
    24961 ggcccttggc ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt
    25021 gcgacacaag tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat
    25081 gcgtacgcaa gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca
    25141 acattgaaag tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat
    25201 aaccttcata gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt
    25261 gtatactaca aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat
    25321 gtactgattg acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag
    25381 gatatgaaaa acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc
    25441 ctccttgact ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata
    25501 attagttatt gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg
    25561 tttttttata ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc
    25621 tcaaaaatat ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt
    25681 gattgctgac actggaaata aatgtctgat tcacttttct aaaatccatt gatttcggca
    25741 gtccattacg acgcagaatc ccgttagaat tctcgtttaa tgggcgttga gaaggtgttc
    25801 cagggtccgc aaaatatata tcaatatcat gttggttact aatggctttc cagttagaaa
    25861 attcttttcc acagtcaaac gtaatagatt taaagaagtt tttagggaag cgagaaaacc
    25921 attgattaag ggcagtttca atatctaatg ccttacggcc gttgggttta atcgtgataa
    25981 tgactttaga taatctttca actaaagtaa tgacggcact tttatgatga atgccaacga
    26041 tcgtatcacc ttcaaggtga ccaaactcag aattgaaatc aggataatta tcaggtcgat
    26101 catggattga gcgctgatac tgttgttttc cccgtttttc ctgatggcca ttgggttttc
    26161 ttttaccttt catcggtagt gtgtcaatat caaatattcc tttagaaaat aaacgataaa
    26221 gtgttctcat accgcatgaa acagaccttt cttttcgccc gataatgacg tcaggcgtcc
    26281 aaccaagagt gactttctct ttaatgtaat ctacttcatg agcaggtaat tgaatgactt
    26341 ttctaccaca ttttttctta ttttctttat actggtgcca ataatcaaga gcagtcttgc
    26401 cttgcttgaa cttattgact acattataaa tagtttgtat agcacgtccc attcggttag
    26461 cgatttcaac cggcttatta tgttgaagat aatatgattc tatcattgtc agttcgtcta
    26521 tggtaagatg tttgtaggtc atttatggtt acactccttt gttttctttc gtcggaaata
    26581 caatttgagt gtaccataaa tgccttttta tttttctaac ttaattttac aattcgcgat
    26641 atattaaaca acgttgtcgc gcttcattga aacattttat gaaaattcca aaaaagagtt
    26701 gacacatgtt tttagacgtg ataagataac taagtcgtta aaaagaacca cttgcgaagc
    26761 ttgattctat cgaaaaaaaa cttcaaaaaa gttcttgaca aataacaaac gataagttat
    26821 gataaatgag ttgctgaaag atttcgaaaa acgaaatcga aaaaaacttt taaaaaagag
    26881 ttgacaatcg cttgtctctc tgatatgata taaaagttgc tacggcagcc aaaaacgaat
    26941 cgggaaacac tcccgatgaa gtagaccttt gaaaactgaa caaagtaaga caaaccaaat
    27001 gtgtagggcg tcttgattca attcaagaca ataaacattt ttaacaagca agcaatatgc
    27061 tagcaaacaa attgagctta acaatcgcaa gattgttcga actttttatg agagtttgat
    27121 cctggctcag gacgaacgct ggcggcgtgc ctaatacatg caagtcgaac gcttcttttt
    27181 ccaccggagc ttgctccacc ggaaaaagag gagtggcgaa cgggtgagta acacgtgggt
    27241 aacctgccca tcagaagggg ataacacttg gaaacaggtg ctaataccgt ataacaatcg
    27301 aaaccgcatg gttttgattt gaaaggcgct tt
//
LOCUS       DACDTO010000029        27265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDTO010000029 DACDTO010000000
VERSION     DACDTO010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.029"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..194)
                     /locus_tag="IWK70_001280"
     CDS             complement(<1..194)
                     /locus_tag="IWK70_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9613843.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAG"
     gene            complement(231..683)
                     /locus_tag="IWK70_001281"
     CDS             complement(231..683)
                     /locus_tag="IWK70_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9613844.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(818..1423)
                     /locus_tag="IWK70_001282"
     CDS             complement(818..1423)
                     /locus_tag="IWK70_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9613845.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(1873..2493)
                     /locus_tag="IWK70_001283"
     CDS             complement(1873..2493)
                     /locus_tag="IWK70_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9613846.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(2495..3364)
                     /locus_tag="IWK70_001284"
     CDS             complement(2495..3364)
                     /locus_tag="IWK70_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9613847.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(3387..3794)
                     /locus_tag="IWK70_001285"
     CDS             complement(3387..3794)
                     /locus_tag="IWK70_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9613848.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(3813..5102)
                     /locus_tag="IWK70_001286"
     CDS             complement(3813..5102)
                     /locus_tag="IWK70_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9613849.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(5129..5431)
                     /locus_tag="IWK70_001287"
     CDS             complement(5129..5431)
                     /locus_tag="IWK70_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9613850.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(5456..7549)
                     /locus_tag="IWK70_001288"
     CDS             complement(5456..7549)
                     /locus_tag="IWK70_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9613851.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(8038..8802)
                     /locus_tag="IWK70_001289"
     CDS             complement(8038..8802)
                     /locus_tag="IWK70_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9613852.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(8913..9782)
                     /gene="mdcB"
                     /locus_tag="IWK70_001290"
     CDS             complement(8913..9782)
                     /gene="mdcB"
                     /locus_tag="IWK70_001290"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9613853.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(9807..10883)
                     /locus_tag="IWK70_001291"
     CDS             complement(9807..10883)
                     /locus_tag="IWK70_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9613854.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(10907..11500)
                     /locus_tag="IWK70_001292"
     CDS             complement(10907..11500)
                     /locus_tag="IWK70_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9613855.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(11504..13180)
                     /gene="mdcD"
                     /locus_tag="IWK70_001293"
     CDS             complement(11504..13180)
                     /gene="mdcD"
                     /locus_tag="IWK70_001293"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9613856.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(13170..13469)
                     /locus_tag="IWK70_001294"
     CDS             complement(13170..13469)
                     /locus_tag="IWK70_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9613857.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(13571..15217)
                     /gene="mdcA"
                     /locus_tag="IWK70_001295"
     CDS             complement(13571..15217)
                     /gene="mdcA"
                     /locus_tag="IWK70_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9613858.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(15224..16135)
                     /locus_tag="IWK70_001296"
     CDS             complement(15224..16135)
                     /locus_tag="IWK70_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9613859.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            16255..17112
                     /locus_tag="IWK70_001297"
     CDS             16255..17112
                     /locus_tag="IWK70_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9613860.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(17299..17661)
                     /locus_tag="IWK70_001298"
     CDS             complement(17299..17661)
                     /locus_tag="IWK70_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613861.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            18007..19446
                     /locus_tag="IWK70_001299"
     CDS             18007..19446
                     /locus_tag="IWK70_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9613862.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(19453..22005)
                     /locus_tag="IWK70_001300"
     CDS             complement(19453..22005)
                     /locus_tag="IWK70_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9613863.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(22119..23450)
                     /locus_tag="IWK70_001301"
     CDS             complement(22119..23450)
                     /locus_tag="IWK70_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9613864.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(23416..24894)
                     /locus_tag="IWK70_001302"
     CDS             complement(23416..24894)
                     /locus_tag="IWK70_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9613865.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(25199..27034)
                     /gene="tdc"
                     /locus_tag="IWK70_001303"
     CDS             complement(25199..27034)
                     /gene="tdc"
                     /locus_tag="IWK70_001303"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9613866.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
BASE COUNT         7885 a         5804 c         4651 g         8925 t
ORIGIN      
        1 cccgcaggct cttgatcgat actcactagg aaaaaatcgg tttctttatc ttttagctct
       61 tctgtaagtt tttctagtgc ataagcttgt gtcaagtaat cagctaaatg ctccggtgaa
      121 ttatcttttc caaatgtatc tgtaaatgtt tcgatactaa tttgttgtag taagtctaca
      181 tctgaaggcg tcatttttcg aatttcgtat ttattcacgg cttcttcctt tcaatagatc
      241 cttttattgc cttttttgac aaaattccaa tcttcgctaa cattcttgca tactttattt
      301 aaaagatatt ccaattgttt tgcttcttcg tcagaaagac catttaatgc gactgcattg
      361 gagtagttgt tttctcgaat gatgaaagga tagatttctg ctcctttttt agtcggatag
      421 agtttcttga tttttttatt ttctttatct tccaaacgtt cgatcatccc attggattct
      481 aatttcttga ttgctcgggc agtcgttgta cgatctactt tgatcatttc tgctagtttt
      541 tcttggatga ttccaggatt ttcgcaaata cgtactaaat aaagatactg tcctcttgtt
      601 aaatcgactt ctttgaattc gatattgcta atagaatcta gcgctcgggc aatcattccg
      661 atgtctcgaa ggatttcagc catttcatcc cacctttatt ttgtttgtaa gactagttta
      721 cctctttttg ttgcaaatgc aactattttc aataaaaaaa tcaagttctt ttttaataac
      781 tagtaaagta tattcttttc ttcttttacg aaaaagctca atcacaatag atctggtttt
      841 tgttttctaa aataagtaac tgtttcttcg ttgttcgctt ttctgatagc tttggcaact
      901 cttctatact aaaaaatccc atgttggaag tttcattatt ctcaataaac ttggcctctt
      961 caccgtggat cgcacaagca aaaacaagtt tataatactg aaaaagctgt ggaatatctt
     1021 tttgtttatt cgtatcaaaa actgctcgca actgagtagt ttctactgtt aatcctgttt
     1081 cttcatagac ttctttacgc acattttctt caggagacaa tcctatttca gcaaatccac
     1141 caggaagtga ccattctttc gtccgtttat cttcgattag taggaccttg ccatcttttt
     1201 tgatataggc acgaacatct acttttggtg ttggatgtcc ttctccaatc gttgtgagcg
     1261 cttttatttc ttcgaaagtt tcttttccta tttcactgat cagttccaat gaaatcgtcc
     1321 ggatctcctc aaaacgctct ttatcaaata catcttttcc ataatataga ccagcttgag
     1381 aaagtgcgag tagtcttttg tattgagcca acttcttgtt cattctcttc ccttcttttt
     1441 tagcaaatgt atgactatgt aatcaagtat agaagactgg tttaattacg gcaaataaat
     1501 ataatgaaat ctgcaataaa taaaagaatg ttaaaaaact cgagaacaaa agacattcga
     1561 cttctctggg gctgtgaata aatggcgttc aaaagcttgc atctgcggta ataagcccca
     1621 ccaaacgaaa aatatgacga gctattcttg tctggctgtt cttattaatt gaggcaggac
     1681 agctttttca caacctcccc actaaacggt gttctcctac caactcctcg gtcacaagtc
     1741 acaatattca tcaaacatga gaagctgttt ttttgaaatt gctccttgtg actcggagct
     1801 gaacgacagg ctcacgacct ctcggtaaac agcgttcaaa agccaatttt tcgatattaa
     1861 gccatttctt tgttacttat tgtcattgat ttctttttct aatttatcca atagtggagt
     1921 gtaatccact tgagtatagt ctgttacatc aaattcatat agtgttccta actggaattt
     1981 gtcatatttt cggtcggcga tgatcttttt gaatgcttct ttcgtaatat gattggtccc
     2041 tttcgttcga tcttccaata cgtcaccata gtgataatga tccatgatat agctcaaatg
     2101 acgatctgat tcacgatcac gtttgtaccg acgttgacat aacacttcaa agtctgctgt
     2161 taggcgaatc gtgatcacct gatatccgta tctatctgct aattcttgga gtcgtgcttt
     2221 ttgcttatca ctaaaaggat actcggacac aatcactttc tttcctgctt ccatatacaa
     2281 atcaagtgct ttgtaataga aaggataaac tttcgtcctt tcttgttccg cccgttcttc
     2341 caaattgtta aacccgatgg attctgcata gtattccttg atttcatcga gcgtgatggc
     2401 atacatatct ggaaatcgtt cccgtagttt attcataaga tatgtttttc ctgtaccagg
     2461 actaccagcc agaagaatga aatacttttt catctcattt atttcctaag ttaattggac
     2521 tttccaagtt cataaacagt ccaatcttat gcttgacaat ttcttttact gcttcatttg
     2581 ctggaatcac attatggcga agagacttgt tgatttctgt ttcactaaag gtttctttag
     2641 ctactttcgt ccagttgact aaaagctccg ttccaacatt gaatttgcgg atcccgttat
     2701 tgatcaattc aggataatct tcttctttca cacctgttcc tccatggata actaggggga
     2761 catcaactac tgcatcgatt tctttcagca agggaatgtt tacttctgtt tttgatttga
     2821 attgtccatg attcgtaccg atagcaatag ctaatgcatc cacgcctgta gcttctacaa
     2881 atttcactgc gtcttccggt cttgtatata ccttgtcatt ttcagcgaca tggattcctt
     2941 cttctgtacc gccgattgtt ccaagttctc cttctacgga aacaccgtgc gcatgggcat
     3001 attctactac tgcttttgtc ttcaggatat tttctttgaa aggcagatgg gacccatcat
     3061 acatgacaga agtatatccg ctgtcgattg cttctttgat atcatcaaag tctcgggcgt
     3121 ggtctaagtg caagactgca tcaaccattt cttcttctgc aatagctgtc attacattga
     3181 ctaacacatg catgccgata tatttagcgg tatcgacact tgtttgaata atgatcggcg
     3241 aacccatttc ttttgctgcg cctagcatag caggaagcat ttctaggtta tgcgtattaa
     3301 aagctccaac tgtaaaattc aactcttctg ctgtttttgt tacttctttc aatgttgtat
     3361 acatctttta ttgccacctt atctttttat ttgtttcctt tgatcgttac ttgttttgca
     3421 atttgtcgca tctcatccat tttgcccatg tagtaatcga tcccttgatc atcaccagca
     3481 cgagcagcat ctgcacgctt ctggttatag aggctcatca gttttttata cccatctccg
     3541 atcgtctgtt tattttccaa actcttttct aaatattgga tggcttgatc gttcacatac
     3601 aattcagaaa atttaagtcc aacttgttca taaagtttcg ctaatttatg cttatcttgg
     3661 gtagtggaaa tctcttgttt caattgtgta atctctgatt gaatcttttc ggtcgcttct
     3721 ggcgataggg ctttttcttc ttttgccaga acttttttcg gttctttttc ttttttctta
     3781 aaaaatccaa acatcatttt ccctactttc aattaaaatg ctttcaaaac tgggcttaag
     3841 atccaggcat ataagagtgc tgcggctact gtatctacat cggttgctgt agcattgatg
     3901 aatcccatcg aattaaagat cgttactagc aaagcgggca ataatgtaat aaagaaacca
     3961 tgagcaatac cgccaatgat cgctcctcgg cgtccgccaa ctgcgttgcc aaatatccct
     4021 gctgtccctc cggcaaagaa attggttagc ataccaggta aaatcatcgc taagccgaac
     4081 atgggcaaag taaacatggc aataatcgta ccaatcgttg tcgtaatgaa acctaaaatg
     4141 acagcatttg ggctataagg aaagaaaact ggacaatcta aggcagggat cgcatcagga
     4201 actaatttca tagctatccc tcgaaatgcc ggcacgattt cacctaacag tagacgtaca
     4261 ccagctagca atacatacac acccactacg aattggatgg cttgtaaaaa ggcaaacatg
     4321 atatagtttt gatctcctga taattcagaa gcgaatggtt cgcctgcaaa taaaacagtg
     4381 atgatatata gcgggaccat cacgaccatg acagacaaat aagtatcttg cagaaattcg
     4441 aaatgtgtcg gcaacttgat atcttcgatc gactttttat tttcgccttt ctcaccaaaa
     4501 agcttagcaa caccggcttc aaataagtac ccgatcgtac aaaaatgacc taaagcaata
     4561 tcatttgatc cagtgatttt acgaatgatc ggttgcgcta ctgccggcat agcaatggcg
     4621 aaacaagctc cgataaaacc accgaccaag atcaatacga tccctcgcag accggcaaaa
     4681 tagccaaaga ctgtcgtcat cgttgccatc cataaaattg cttgaccagt taaaaaaatg
     4741 tacttccatt ttgtgaaacg agccaaaata atattgaata caaaaatggc taagaacgtc
     4801 agtgcaatat ctctacctaa acccaaatca ttcattgctt gaccattgat agattcaata
     4861 gaagggatga tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa
     4921 cttccaacga tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac
     4981 gtgccagaga tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc
     5041 aatgcaatgg tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgccc
     5101 atgtttttac cctcttttct ttttgtattt agataactcc cttatcttgc agaactgggg
     5161 tgattttttc ttcaatttca ttttttgaaa caatattttt caaatagatc atcgccgttt
     5221 taggatcaac tgcgaacttt tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat
     5281 ctggtttcat ggaaactgcg gaagaaatat cacaatggtc cagtttggct tctactccta
     5341 atttgttcaa aacatcctca gcagacatct gtgctgcaaa actacttcct aaacctgcac
     5401 cgcatacgaa taaaatgttc aatttgttca tctttgttct cccccgtatc atttattatt
     5461 tctcaactac tgaaaaaagc agttgtttga aatcttcgat atttgtgcat tcaatcaatc
     5521 gatccaactt ctcttcgttg ttgatcaatt caattagttc tttcatgatg ttcagatgcg
     5581 agtaggagtc tactgctgcc aaacaaaaaa taatctttac tggatccatt tcaggattac
     5641 cgaaatcgat tggttgctca atcgtagcta cgctgactcc taatttattg accccatctt
     5701 ctggtcgggc atgggctagt gccatgtgtt tgccaatcac gatatacggt ccgtaatcat
     5761 tcactgcacg aatcatcgct tcgacatagc gctcttcgac aatgttttct ttaaccaatg
     5821 gtctagcagc tagttcaatc gattcctccc aagtagtggc tttttctttg atcatgatat
     5881 tgctgtccgt caaaatatct tttaacatag gctggatctc ccttttgttg atttctaaat
     5941 tgtttgtttc gaataatttt tctaacttgc catagatgat tcgatctact cggccaccac
     6001 tttctttcat gacatctatt atttgagtga ataactccgt cgattcatga ctgttaggct
     6061 ctaaccgctg tagttcttca tttcttttca aaaagtcttc tataaccaag atattcgtct
     6121 cagttaaaat cggatcgaca actaaaagcg gcttatttcg ataattcaat tgaaaggtgc
     6181 tgaaaaccag attgacatcc aatttatcta ctaattcgat ttcttttgaa cttaaaactg
     6241 cagtaatctc gatctgagga aacttttctt tcagattctc tgctaacaga tttccagtag
     6301 ccatcccatg gttacagaca acgactgatt tataaacata attcaagtct cgcttgatta
     6361 cactggcgat cgtcgaaaag tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc
     6421 caccaattac ttcttcgatc gtaggaacaa aatgagtgac tgcgcggtag attgggaaat
     6481 aatttgttcg gatgttttct ttcaatggat tcatgacttg gatatgattc ttgattctta
     6541 caatcagtgc agccatgtgc ttatagagat tttcgaataa agcttcttcc tttagatgga
     6601 aaggtacgtg tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt
     6661 gtacccactc gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa
     6721 gataatttct ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa
     6781 taaatgcaga aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa
     6841 attcatgttt cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat
     6901 aaatatcatc ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt
     6961 ccaccaaagg aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat
     7021 tcataaagaa gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga
     7081 tagatcgttc tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc
     7141 cataactgac cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga
     7201 tttgaaattc ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta
     7261 aaactagatc aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt
     7321 cagtttctaa tcgagcttgc ttttcctttt ccagcttcaa caaaaaaact ttgttgcgaa
     7381 ttgtacggat ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct
     7441 gacggatcgt ccgctgactg acattgaact cacttgctag atgttttgct tttactggat
     7501 attgggctgt catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct
     7561 ccttctgatt tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact
     7621 tgaagtggaa agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc
     7681 tcaacgaaaa agacatacgc ctattattac aaaaattttg cccatctata caaaaaagag
     7741 gttgtgaaaa agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc
     7801 tatttttcgt ttggttgttc ttattatcgc agatgcaagc ttttaaacac catttagtgg
     7861 agaggttgtg acagagatgt tcagccttga gcgataagaa gcgattttga aaaatcgctt
     7921 cccatatttt acaaaatcgt gccttattgc cgataggcta tctctggcac actgtttatt
     7981 cagaaaaagg agctgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca
     8041 atccagcata atcggctgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc
     8101 aaaataccat atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc
     8161 atcttttcga tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt
     8221 ttcgtggatc tgtcgataat ttcctacttc ctgaatagtt atttttttag tatgatttct
     8281 tacaccgtct gctttgtttt tttgcagaac tttctgatgt tcggcaaaca gcttgtctgt
     8341 ataacagctt ctggcttttt caggagtttc ttcttcccaa gcggattgga tcttcatgaa
     8401 tatcttttgg atttccagaa actgtgcttt cttttcttta gccgttcctg acatgcggga
     8461 caatatctcc gctttcagcc ttttcttttg tcttttattc ttgacgatag aaaaagcaga
     8521 aacccctcct actgacaaaa cagctaatgc aagaaatccc gtttgctcat aactagaagg
     8581 acctgtacct ggaccataat acccataaga atctctgccg taagaacgac tgccaaaata
     8641 actgccccca tatgaacgac caccaaatga acctctgccc gaacttcgac cgctagatgt
     8701 acctccacca tgaccacccg cgacagcgaa tccttgggtc ggcagagcaa acaaacatag
     8761 gcccactaac aaaatgattg ctaaataatt gatttttttc attgtcttcc tcgttatctt
     8821 ttattacaaa aaactatttt tatgatagcc ttgctaaaag gaagagtcaa taaccagatg
     8881 cataaactgt ttttttagca attttgtctt atttagtcat tgagtaacat cggacaaatg
     8941 tatgtgtcgg cagtccataa ttcctctatc ttcagcatga acaagcttgc agacaagaga
     9001 tctgcgcttc ctccaggaga gatttggttt tcctgacaaa agtgcaacag tgctgtaaat
     9061 ggacgatttg gtaaagcact ttgactagca gcatgagcaa gtttttgcat ttccactaac
     9121 gttttctgat cactgcgatg caatacgcat gtgtcatcta gcgaactcat caaaactaac
     9181 aacatatgca tttgtttctg tacatatgaa tcattatcta gctccttagc tgcttggatt
     9241 gcaagcctga tatgcggata ccctaatatc gcttcttcat atgcaccatt tacgccatat
     9301 ttttgcttca ccctatgacc atgagtggtt tttttatgac gatattttgt atcaggaaaa
     9361 gcagcaagct tgctgacatc atcaagtatt ttcgataaag aaaaaggctg ttccatactg
     9421 attttttgtg caatgacact gatcaatagc cccattgccc aaatcgctcc tttatgggta
     9481 ttgatgccat ttgtagcaat cagcattgcc ttttccgctt ttcgcccaat ctcagcaatt
     9541 ctttctctta actcctgatc aatagcatga tgccatgcta tttgagccat ttcttcaaaa
     9601 aaaggagtta gcgtcttagc agacttaaga aataaagcat agtccatatc ggaatgtgca
     9661 cccgcatcca gtgcatccac taatccaggt tttggactta atgttacttc tgcgatcaat
     9721 gcttcctcta ccaacgcagc gagttgctga gtcactgctt tttttcgttc gtcatttatc
     9781 atgacactca ctccttcttc ctactattac ttgctgaaac cttttttgat agctacccaa
     9841 atgatatgaa gtaaccagct taaagcaaat gcacaacttg ccgtcattag ataagctaca
     9901 agaatcatga ctcctgaagg taactgcagc caactatctt taaacaataa taacagtact
     9961 tggaaaacaa atacattggc taaatacgta cgataagcat aaacagacaa ccatttgaac
    10021 aatggcaatt ttggcgaatc gtttacaatc attttggaag cgcctatgaa gacagcaaag
    10081 atgatgacta aactgtataa actttggatc gtattcaagt aaggtgcatt cgcaaagctc
    10141 aagtctcctg gatgtgcgag caatgaacgg acagaaaaga acgcgataac taaggcaaca
    10201 ggtaagaaaa gataacgtac tctgtccaaa tattgataga ctttttcgtg ataaacaaat
    10261 gcggctaccc cataaatcat gaaactagca acaaaccgat ctaataagta ccagctgtca
    10321 tgataaggcc cagtcaatac ttgtgtttga tagaacagat accatgcgac atataaaatc
    10381 gttgaaccaa tcaatactgg ccatacatat tttttattag caaatacttt gtaaccgagc
    10441 caaacaaaaa acggcatgaa caattggatc tgcagcatca taacggtata ccataaatga
    10501 ggcgctccat cacctgtgac atatttcagt aaaaagcttg cagtatccgt atacggagtt
    10561 tgttgttgga ctgctggaaa tagccaaaga taagccgtcg tccatagaag atacggaacg
    10621 actagttcaa aaaacttttc tttcaaaaat tcccaataag acaaatgctg acttgttttt
    10681 accatattat aaacaattgc aaaaataaac attggtgctg tgtatttaac gatgacgtaa
    10741 aaggtaccaa gaaaagctgt atttgttgcg ctgtggctgt ttgccaaagc aaagctcaat
    10801 atcgtttgca tcataacagc cgttacagct gatactttct ggaagtcact tacgttgatt
    10861 ttgatttttt tgttttcaaa catctctttc tctctctttt tctttattac caaagttctt
    10921 tggttaattt aggtccttcg actgttttca atagaatttt tttctctggc tctctcaaat
    10981 actcttttag tgaaaaaccg ccttgccgag tcactacttg ggtatcgaat tgctcaaaaa
    11041 aagcaggatg cgattgaatt acctgaattg gcaattcaat cggtgaattt gcgtacagca
    11101 gcaaatcaaa atcactggtt tcttttactg ccggaatccc agtcgataat tcaaatccta
    11161 agctgccgcc gattccccat ttacatcctg ataatatgtt tctagcagca tgaaattgct
    11221 gataaacagg ataatccatg attttctctt gtctatacga ttcttgttcg atgatgtccc
    11281 acggatgaat gacttgtttg acggcatgtt ttgagatttc tgttgaccat ctctgattgc
    11341 gggcaggtcc acgtaaacca atcggtagta tttctgcgtg atccgtcgac gcacgacgaa
    11401 ctgttccgaa catcttttca gttagaacga cccattcagg caaggtgaac ccttctattt
    11461 catgtttatc aaaaaggaca atatcatgtg cttgaatcat tttttatgca tcccattctt
    11521 cgtccatttt ggcacggact ttatttgttt cgacacgtcc gaatgttttg gctgtttcgt
    11581 ttgtgtatcg atttgtcagc atcgtgtcat ttgtattgcg gacatcttga atggcttcat
    11641 tgattgcttc gatgactttg ctgacatttt gttctgaagc agcttggtcg gtgatatctt
    11701 ctaggaaatg atagatcgct cctagttttg tataattatt gatatcgtaa gcgatagctg
    11761 gaactttttc agcagctgct tcgatttcag caaccgaacg ttttgtcaca cgtgcagcac
    11821 tttctttaga catcgcttgg acggtaattt ctttattgtt taaagcaatc aagcggcttg
    11881 attgtaatcc atgtgctaaa aatgcacctg aaatagcatt tcctgcaatc aatccaataa
    11941 ctggatgtcc agcttgacgg agttttgcat aagcagcagc actattagcc aaagccacat
    12001 ggataccgat caattcttcg ttatagccgt atgcttggct tggtacatcg atcacaacga
    12061 tgattggccg tttcactttt ttgtctttat cttcttcgta aacgtgatcc aaaatttgtc
    12121 ccatgcggaa accttcaact aaaccaactt caccgtttcg tacacggtac atcgggtttt
    12181 taggatcagg tacgattgat gtcacgatgg cttcttctat gaatttactt gatttaccat
    12241 aatacacagt actaatagaa gaagttggat ttttcatgcc agttagtgcc gcaaaccaat
    12301 cgtaaccaac acttgctgca gctggttcat ttccttctgc tacttctggt aagtcaattg
    12361 attttgcttg atggttagca attgcttcat tgtaatcgtc aatggccatt ggttttgaaa
    12421 gatctaaaga atcaagtaat gataaataaa atgctgagct ttcactgcga tgcgtgtctt
    12481 ttcgttcgtc cattgctgcg atgacagcag ctttgattgc ttctacacta tcagtcacta
    12541 attcatcaat gatttttgat tctacccgtt gacgagatcc taatgtattc caaatcaagt
    12601 ctttgtcact agaatcaaat tctcgaacgc ctgcttcttg ttcaataact tctggtccat
    12661 tcaaaccgac tctcgctttt tttgttgcga ttaaataact cattaatgct gaagtaatcg
    12721 acataccgcc aaatgatcct acacgtcctg gaatcaaacc gatgactggt acatattgtt
    12781 ttagagcaat gattacattt ccgatttctg aaatagataa cagtccgtag tttgcttctt
    12841 gcaaacgaac accacctgta tctagtacaa tgactggaaa gatctcattt ccttttttat
    12901 tttcttctaa cacatgttca agtgcagcaa cgatttttgc accggaaact tcaccgatac
    12961 cgccaccttg gaattttcct tccatagcga ttactactgc ttgtttgcca ttgatcgtgc
    13021 cacggctgac aacgatcccg tcatcacttt cagggacgat tccttgtgct ggtaaacttg
    13081 gcgcgatgat ttgatcgaat ggatcaatca attcacgtcc attgtcaagt agagcgttca
    13141 ctcgttctct ggcgtttaat tctacaaaac tatttttcat ctgaaagttc ctccattgct
    13201 tgcgtcaatc gtaggtacac aactcctggt gtagcaccga agtcattgat cacgatatct
    13261 gctgaaattg gataacgatc aaaaaatctc gttaatacat tgttccatac ttcgccgaag
    13321 ccatcgcttc ctgtacaaat ttttacagta gattctgttt tttctgttgg aaacatcaag
    13381 atttccaaat ctcctgaacc gaccactcct acatggattt tcttattgat cggctgtttc
    13441 gttggatatt tataagctaa ttgttccatg gatctctccc ctttttgtaa aacagagcat
    13501 acacgttctg cttattcgtc tttcttcttc catctgggta aatggcgaca aaaggttttt
    13561 ctcgccaatt ttatgaccaa gttctgaatt tcattggtgg ttcatacagg ccatctgacc
    13621 attcgatcaa atcatcgatg gtttgtgcag ctaataatga acgtttcgct tcactacgtt
    13681 tgatgcctag atcttctggt aatttcacga tgccggctgc acgcaagcta tctagtgatt
    13741 ctttcgtgct agtcattcca agaggtgtaa caccgccaat tgctgcaatt gctgcttgtc
    13801 gttcttcttt tgtttttgct ttgtacaaat aagcaattcc ttcttctgtc acgatatgtg
    13861 tagtatcttc gctataaatc atcactggtg cattggctaa gcctgcttgt ttttgaacag
    13921 ctagtgcatc taattcttcg acaaagactg gttttttatt tgaaccatat gtttcaacca
    13981 tttgaacaac taatttttgg cctttcccta atggatcatc attgtcgcga agtgattgcc
    14041 atgctggtgt agaatgacga cgaccgccag gattatgtcc catgttaggc gcaccgccaa
    14101 aaccagataa acgtcctttt gttacggtag aagagtttcc ttcgtaatct aattgcaagg
    14161 ttgaaccgat aaacatatcg atggcatatt gtcctgcagc ttgtgataat gttcggttac
    14221 tgcgcatcgt gccgtcttta ccgacaaaaa agacatctgg gcgagcagca atatattttt
    14281 ccataccaac ttcgccacca aaactatgga tgctttcaac ccaacctgat tcgatggctg
    14341 gaatcatcgt tggatgtggg ttcaataccc agttcttcgc gattttccct tttaacccta
    14401 gtgattcacc ataagttggc agtaacaatt cgatagctgc tgtattgaat ccaataccgt
    14461 gattcaaaga ttgtacgccg tgttctgcgt agattccttt gattgccatc attgccatta
    14521 gaatttgcaa ttctgtgatg ttttgaggat ctcgtgtgaa taaagcttct aattgataag
    14581 gtttatctgc tttcacgaca acgtctaccc agtcaccagg gatatctaca cgtggtaaag
    14641 aatcgacaac ttcgttgact tgaacgatca cgataccatc tttgaaagct gcagcttcga
    14701 tgattgttgg tgtttcttcc gtattgaaac ctgtgtacaa gttgccgtct ttatctgctt
    14761 tatctgcagc tactaaaaca acatttggaa tcaaatcgat gaataagcgt ccataaagtt
    14821 ccaaataagt atggatatca cctaatttca ttgtgccgtc ttcaatcatt tgggcgacac
    14881 gcaagctttg tgcaccagca taagagaaat cgatcttgct tgcgattcct ttttcaaaaa
    14941 tattcaaatg ttctggtcgt gagatactag acatgatcaa atgcagatca ttcacttttt
    15001 ctgcatctac tttttcaaga ctttctgaaa gaaaacttgc ttgtttttga ttgtcgcctt
    15061 ctaatacaac tttgtcgccg ggagcaatca gtttttccat tacttctacg attttttcag
    15121 tttctacata cttgccattg agcaagttgc tgattttttc caatcgctgc gctttttttt
    15181 cgcgtttaga agcccagttg cggtttggtt tttccataat tagtcatctc catttttgat
    15241 atagaaacgt ggcgtcatca ataccatgtt ggtctagatt gatcgtacgg atccctttat
    15301 ctacaccaaa ttttgtatga atcagctttg tcaaagtatt gccgggggga aattcaatcg
    15361 tcagatccat tcctaattct tttgccaagt ccatcatttg gttccactgc acagggaaag
    15421 ctaggttttc caacaaatct ttacgaatct cttctgcgtc ctgagtggct cttccatccg
    15481 tattttttat atacaaacat ctagcatcag atagatggac agactgcata tacggcttga
    15541 atttcttgac cgtatcattc atcaagagac agtgagatgg cacagggact ttcagcagct
    15601 tcgctgtttg cgcttggtgc cgtttggctt ctgccaacgt aacggataaa ccttcgatac
    15661 tcccagataa cgtatgttgc atcggacaat tttcatttga cagataaact ggatggtcag
    15721 gatcaaatgt ttttgctaca atttcctcag cttcgcttct cgtcacacct actactacgc
    15781 ccattgcata accgcttgga taagcttctt tcattaagac tgcgcggtga tagactagtt
    15841 ccgctgcatt ctcaaatctc aaagatcgac aagcaacagc tgcacaaaaa gcaccaatcg
    15901 aatgtcctgc tgcaatatca ggctgaatac cgtttttttc catttcatct aaataaaata
    15961 ctgcttttgt cagtaatgct agctggataa aaaccgtatc ttgatagttt tcttctttat
    16021 caactaaccg ataccccgtc acattttcta aacgttccat gtacatttca gggatatccg
    16081 ctaacatccc taacttctga ctgccttgtc caggaaataa gaaacctgta ctcatgagcc
    16141 tcgcctcctc agcacttact ttttccactg ctatcttagt caatttctgc tataataaaa
    16201 aatgccaaaa aaagaaaaaa ccataacaaa aaggtatgga tgggatggat aattatgaaa
    16261 ttgcttcatc tgcagtattt taaaaaagta ttcgaaacaa aaaatgttac ccaagcagcc
    16321 aaagaattat ttatttctca acctgccttg agccgtgcga tcaaacattt ggaaaacgaa
    16381 ttaggtgtcc ctttgtttta ccatagcggc agaaacattg agacgaccgt ttatgcagaa
    16441 gaattttatc catatgcagt caagacatta gacacattag aagaaggaat caaattattg
    16501 tcttcggtca atgagaagat cgttacttcc gtcatattgt atttagaggt agcctctgtt
    16561 tctattccga acttggtccg aatttttatg gaaaagcatc ctgatatcca attaacgatc
    16621 atgcaacatc atttaccaga tgatattacc gatcctgtac tgtacatcac ttcggagatg
    16681 aagcctggca tgaccaatat tcctatcatg aaagaacctg tttatgtcgc cattcctaag
    16741 aaacatcctc ttgctaaaaa agaacatctg caattagaag atatccaacg cacccccatc
    16801 ttgatgctgt caaagagcaa cgccttccgt catacactgg atgcagcact tgaagcgaaa
    16861 agtatcgaat tgacgatcag ttcgacgaca gatgatcctg cgacattacg ttctattgtc
    16921 aagcaaggat tagctgttag ctttttccca aaagtttctt ggtcttacga taaaaatgat
    16981 cctttcgtct tgcgggaaat caaagatttg cccttgacac gaacaattta tttatcttct
    17041 agcttcacag aagatcatcc tttgatcaaa atgattgcaa aaacgatgcg ggaattttat
    17101 ttaggaaaat aaaaaaaaaa gagctgggac aaaattcttg tcgcagctct tttttcgaac
    17161 aaacggtgtt ccagaagtaa tttcttagaa aataagccga aatttcccaa aaatttgaga
    17221 agcaattttc gtaaattcct tcttatttct tgaagctaaa catttctgtc acaacctctt
    17281 ctctattgtc ctagacattc attgcgttga tttaaaactt ccatcaatca aatctgctag
    17341 ttcatctagc ttttctattt tacgtagatc aatagagatc caatgttctt tattcatgtg
    17401 ataggctgga aaatacccct cttttcctcg caaaattccg ccaagttcag gatcgacctt
    17461 caaatcaaga atattgatga tcctatggtc acttgcatca tcatagattt tttcttctgg
    17521 aacagacatc aggaccccaa accatttttt gcttttttga tgtctgaata cgcaataatc
    17581 aggaaatttt ttaaaaatat attctggaga caaattgtat gtttcattga tgtgatcgat
    17641 tagttctttt ttcgttagca tctgcttcca tctctttctt attgattcgc tttattcaaa
    17701 gataccacgt aagaatagaa aaatgaatac aagtagttgc taatcaaata cttcgatatt
    17761 tttctttttt atcaactctt aaatgttcaa tccgcctgtg ttggtcttgc taccatctac
    17821 tattaaaatg tttattattg tttgcccacc tattttttga ttttagtggt atcatttttc
    17881 aagttcatga tgaaattctt atcatcagta aatgctccag aaaaataaac aatgattgaa
    17941 aaatcagttg aaacttgacc accgtttatt ttctccgtat tcttagaata gaagggagtc
    18001 tgattgatgt attcactaat gaaaaaaatc attaccgaaa aagatattcg acgtcatgtt
    18061 tctttagtag aacaattgct gaatcataca aagataaccg taaatgaatt agctgaaatc
    18121 atagggacaa ccgaacgaac gattttttct gaccttcaat ctattcgctc tcagctgccc
    18181 gaaggctggg acatcatcag cgatcaagca ggaatcagtt tgcaaaacca gcaaaattta
    18241 ttaacaaatg acctttggga gatttttttc aaacaatcag tcagtgtcga actgctgaaa
    18301 aatctgcttt ttacaaaaaa agttgctgtc cctgattttc tagccgacca tgggctttct
    18361 tatgggacat tgaaaagaca tgtgactaaa atcaatcagc gtttagcaag ttacgacttg
    18421 cagatcgatt tgacgaaata tacagcttgt attcttggaa aagagcgtgc gattcgtact
    18481 ttttatcatc gattgctgat tcctttcact cataacaact attttttcga ggattattcg
    18541 atccatgaat cccattattt tcaattttta agaaacctca gtcaaactga actagcggtt
    18601 gaaacagaag agattttcgg aacatgttgg ttctttatca atacgatccg tataaaagcg
    18661 aattgccgat tagattcttc tatccatacc aacagcacac tctcctccct gtatgactct
    18721 gcattgaaaa aattgtattt aacggaaggt atttatctta aagacactga gctttctttt
    18781 gcttctttct gctttctgga aagttggaat tacaacaata actatggaca agaaaccgct
    18841 cgctgcctgc atcattctcc atttttagag gtcctcgaaa cgtttgtaga ggaactagct
    18901 tcagagctgt ctcttgatca actaaaaaaa acatctctga ctgataactt ggctctcctc
    18961 atattaaaat atcatgaatc gccgatattg attgaagcat tggaccgtca ataccatcgt
    19021 tttttagaaa catatgatca gcaatatgca cacttgtatc aacagaaaga gaatttactg
    19081 aacgatctaa aaaaagaaat agaaatcaat gaaccaaatt actttctcca actcttgtct
    19141 ctactgatcc aacaaacgat tttttctatc aaaccgaatt tgttcaatgt ctattttatt
    19201 ttccaaggag aaccttcatg gaaggcgttc ttgcagcagg aattaaaaga ttacttagga
    19261 aaacgtgtct gcttcttacc tatcgaacta accgatcttt ccactatttc ttttgaaaaa
    19321 acagatatct tgatttccaa tttccctcta gattctctag atatccctat tgtatacatt
    19381 tcttctattc caacgaaaaa tgagctcaat cgtttgactg aactcacttt caaaactttt
    19441 ttatagtaac ggctagcgac tgtattctct agctgttttt tcttttttgc gaaacagttt
    19501 gttcatgatc cctttttcta tttctacaac taagaataga agaatcacat ttgctccaat
    19561 caataactgt tgtgtcatcg ttaaacttac tgtaccgatc agctgctggc cgactggtag
    19621 aaaaataacc atcccttgca ataaagcaag gatacctaat gaaagaaaaa gcgctttgtt
    19681 ttgcaaaaga ccttggttca atgacggctt ggacaactct ctgcaattga tcatgtaaac
    19741 agcttgagct aagacgatat tttgcagaag aatggtttgc tgtaagcttt gcccttcaaa
    19801 ctgcatagcc aataaatagg caggaaccat gatcagtagg gagacataga tgatccgaaa
    19861 caaatgataa ccagatagga ttccttgctt tggatctctt ggtgggcgct tcatgatttc
    19921 tggatctggc gactcgaacc ctagtgcata agataaagtg attgtcgtta ccatattgac
    19981 ccataaaatc tgtactgggg acaaaggtag cgggcgattc agcataagtg cccaaatcaa
    20041 gatcaatcct tgagctaatg cagttggcaa gaagaaattg atggtttttt gcaaattatc
    20101 caaaatccgc cggccctctt ttacggcttt tgcaatcgta tggaaattat catctcctaa
    20161 aaccatatca gctgcctgtt tgcttacttc actacctttg attcccatag caattcctac
    20221 atcggatctt ttcaaagcag gtgcatcatt tacgccgtct ccggtcattc cgacgatttc
    20281 tccatttttc tgcaaagctt taacaattcg cagtttatgt tctggtgtcg ttcgagcaaa
    20341 gacatcgact attggcactt gctgaatcaa ctcttcatca gacataagat ccaaatcgat
    20401 cccttctaaa actttggcag tatgttttaa gccaatttgt tccgcaatcg cttttgctgt
    20461 gtctttgtga tcccctgtaa tcattttcac tgagatccct gcttgttggc attcctttac
    20521 agctttgact gcactttctt ttggcggatc gatcaatccg gcgatccctg caaaagttaa
    20581 ttgggttaaa gtctcatgcg tgagttcctg tttcgaatct acttttttat aagcaaatcc
    20641 taaaacacgc tgaccttttc tcgcaaattc tgcagctgtc tgactccatg cttcttgttc
    20701 tgcagaagaa agagttgatt tagcaaataa tacttctggt gcacctttga cgtaaatcac
    20761 cgctcctttt tcctccgcat gcatcgttgc catatatttg tattcagaac tgaacggaat
    20821 ctttgcttct atttttttca aaggtagatt ggctgtttct gtgtgtcgaa tcagcgcaac
    20881 ttctgtcgga ttgccactta aattttctgt tttctgttct tcttttgttt ttaattcctg
    20941 acagtttttc ataatggaca acacgcaggc ttcttctttt gcagctacat ccacaactgt
    21001 catttcattt tttgtcaatg ttcctgtctt atctgaacag atcactgtca tagaacctaa
    21061 tgtttctacc gaaggaagtc ctttgatgat cgccttttct tttgccattt catgtactcc
    21121 aacggataga atcatggtca aaacagctgg caatccttcg ggtaccattg acaccaccaa
    21181 agcaatcgta gccgaaagaa ggatatgcca atccatacca tatcgaaatg tcgtaaaaat
    21241 aactaagaat aggctcaaac aaagaatgcc ttgaaaaatt tgtttgttta atcggttgat
    21301 ttttttaatc agaggcgtcg tttgttgttc gaccgattgc aatgcttgat tgattttacc
    21361 gatttctgtc gtgtttcctg tttctacaac gacaccgact gcagaaccag attgtacaag
    21421 cgttcctgaa aaagccatat ttttctggtc gccgatcgat tcttcttctg tcaaaacggc
    21481 atgacttttt tcagatgctt ctgattctcc cgtcaaaatc gattcttcca ctaataaatc
    21541 atgtgcttcg attaatcgga tatctgctgg aacgacatct ccggcacgta agacaatgac
    21601 atctcctttt accagttctt tcgcagggat tttttcttgc atcccgtttg atagtactat
    21661 cgcttcttgt cccatcagct gcttcaaacc attcaatgat tcttccgctt ttctttcttg
    21721 ccagtaacta acgagtccat tgacgatcac tacgaataaa ataatacttc cttcaatata
    21781 ttcactagaa gcaaatttca aaaaagaggc aatgatcaac acgatcatca gcagatcagt
    21841 aaaatgcttt gctatttttt tccatcgttt tgtctgctgc cttgctttta tttcattata
    21901 tccttgatca gtcaggcgtt tctgtcgttc ttcttgcgac aagccttctg ttgatgtttg
    21961 atagtttttc aatacatctt ctaccgtcaa tctgtaccag ttcatcctat cccctcctgt
    22021 ttaattgata ctgttattat acgcagggaa gttttttcag gcgtatcgat tatttcaggg
    22081 gggtctgaaa taattcgttt tgcaaaatgt atctgggcct aactgatatt tctattgctt
    22141 attcttttat atacaaaatt taagaataca aaccagataa ccaataaaaa aggaataaag
    22201 cgtgtcgtgc tttcgaataa caagctgaaa gcaattgaag taaaaaacac caacaaagca
    22261 agatctttat atctttgtgt agtgttccgt ttcttttctt taataaattg aacatgtgca
    22321 cacaagatcg aagaccacac aaatagaaaa caactcgttg tcatgcctgc caaaagctga
    22381 aaaaacaatc gactatcagc tacaaaaaaa tgtagacaaa gaccggcaaa aaataacatt
    22441 cctgtaatta aaataccgtg taaaggtacg cctttcttag aaagcgcaca aaaccaatgg
    22501 ggcaaacttt tttcttttgc tgtttgatat aaaatcctgc tagtactgta gactgcacta
    22561 tttgctgagg acaaagaagc aaccaaaatc attagattga caattgctgc tccgccttgg
    22621 caatggatca cttgcagcat agtcactaaa ggactttctt ttaaatctaa acaatgccat
    22681 ggaacaacaa ataaaatcga caaaatcgtt cctatataga aaaagagaat acgccaaggc
    22741 agacagttga ttgctttttt caaattcttt tgcggatcac ttgtctcgcc aacggttaag
    22801 ccgatcattt caattccagc aaaactgaat aaaaccatag gaaatgagac taccattcca
    22861 gcaaagccaa acggtagtaa ttcatcgatc tgtccaaaat tttctttgat tccgatagac
    22921 tgggtatgaa aatctgagac actcaatcct atacccaata agataaaaag gaaaatcccc
    22981 aagatcttga tgattgagaa aaaagattcc aattcaccaa accacttgac tgcaacaagg
    23041 ttgactgcca ttaagatcat tagtgcgcta cctccgctta tcaaagctgg tacatgtaca
    23101 aaccagaaat ggatatacaa tcctattgct gttaattccg caagtccagc tccaagccag
    23161 caaagccaat aagtccaatt gacaaaaaat gcccactttt tgcctaaata atagtcaacg
    23221 aaatcactga tagagttaaa tgtactattt gctaaaaata aatcacccat ggcccgcatc
    23281 aaaccataaa tagccaaacc gcatatcaaa taaacgagaa taacagctgg ccctgctgat
    23341 gcaattgcct ttgctgaacc taaaaataat cctgttccaa ttgcgccacc taatgcgatt
    23401 aactggatat ttcgattagt tagagtccgt ttcattgccg gtttttccat caagttcacc
    23461 tttttctttt tcaagcagca tggcgacttc ctgttcccaa gaaggtttct ttattttttc
    23521 aatacagagt ggtactacaa acaaaatgac actgatggct gtgactgcaa ttagattgat
    23581 ccatgttcca gctttcatcg tcagtccgac agaaaatccg attgccgcaa ataaaacaat
    23641 aacgacagtc caaactccaa gattgctctt tttctctttt tttccaattc taaatggtcg
    23701 attcaacttt gcatgtgtat aacgcaatct caaaactgcg atccccatga tgacatagac
    23761 gatacagtag ataacagtag ttgaatttac gagcatcaaa aatgcttgat tgacgccagg
    23821 aatcagcaaa taaaaaagtg caaagattga gataatgaca gactgagtca aaataacgga
    23881 tttggatacg tcaaattgat tcgtcttcca aaaattgaat tttggtggat acgcaccttt
    23941 tctagcactt tctgtcactg tctttgctgg tcctgaggcc caagcagata attggactgc
    24001 tacaccaatg aagacaagta aactaaacag attcacaata agatgtggca aaccaagaat
    24061 ttggcaataa atcgaaatag attgcgcgat attattcaat tccatttgtc cttttggtac
    24121 gacatttgct acaactaaag cattcaaaat attgacgaaa aatataaaaa ttaacgcggc
    24181 aaatactccg cgaatatacg ttttaactgg attttccaat cgagtgatat acacagagga
    24241 catttcgata ccagtaaaaa taaacatgat cggcgcaaaa taaacaaatg aggatgcagt
    24301 cgtagtatct ggtattaatt tgtcccatga aaaactgcct aatgttccgg ttggtacgat
    24361 cccaactttg atccacgcag caaaccctaa taagagcatc atcgtcaaag gcacgtacaa
    24421 tcctaaccaa acaccgactt ttccgccgat ttttgccata tcaaatttca aattcaaaag
    24481 agtgatgatc caataaacga ctaaaatgac aactaatgta aatttgctgt tcaaaccaag
    24541 cggcacatta tcgataatat taccaaacaa cggagcgagt gctgaagcga ccatcaccat
    24601 cccaggaaac atttggaccc ataacagcca tgagacaacg aacccccatt tttttcctag
    24661 agcattcgat acccaaagtt ctggaccacc tttttgggga aacattccag caaattcacc
    24721 agagatcaat gagattggaa aagcgtacaa taatactgca accagcatgt aaaaaatcat
    24781 cgtccagctc gttgctgcta catctggaat atttcgtaca cttgctacaa gggcgcaggt
    24841 cattccgata aaggtcatgg tcgtgatttg ctttgatgaa ccagtatctt tcatctttat
    24901 tccttctttc aatagcgaca tatgtttttg ttttctttta tttttgttca agaacagaaa
    24961 tgacaaggtt tcatcgattc agtacaaaac ccagtatcac cgaaacatcc attgaacgat
    25021 cttagtgaac tgggtttgat acatgaaacg aacgatccaa cttatagaac gttttataaa
    25081 aaagaatatc ttggcgagga atatatgaaa aacaggggga aatacaattg ttttgatgaa
    25141 gcaaataatt gtctgttctc catatgtctc ttgttattct ttcacacaaa aatcagttct
    25201 attggatgat ttcgtttagt ttcttttcca ttgctttggc gatttttttt gtataaaaat
    25261 tgaaaattcg atcatcattc aaataaggag tcatgatagc cgcacgtagc agagtgattt
    25321 tctgttcttt tttccattct tctttcgtta gacccattct ttcaacaaaa cgaatcggag
    25381 aatcaccgta atcttcttgt gtaaatgtag tatgagaagt aataaaccgt tcaccataaa
    25441 catcgccatc catataagaa gaccgatcga acattttttc atttaattca ttgatagcgt
    25501 tcaaatctgt acagccttgt tctttgaata cccaattgac catattaaaa tcaggatgat
    25561 tcaatggata gacttcgatt gtttttcctt taacagtaaa agtcagatga tcgagaaatt
    25621 ctctgaatct ttgagctgcc tcgattgaag ccccaatcaa ttgaccatat ccggtcacat
    25681 tcaatggcaa tacacgatgt gctgtccaga ctgcggcggc ggttgctcca gcttttgatc
    25741 cttccaaaat ataagctcct aacatatctg gagcctttac tgatttttca aacacatatg
    25801 gcgcgaaata agaaatgata tttctcatat ttttatgttt gattacgatt cctccagcag
    25861 cataagggac atagcccatc ttatgaggat ctatcgtgac agaatccgct tctgaaatag
    25921 ctctgaaccc ttcatatacc tctttgtcaa tcgttacgca gtggtggaaa acatgatgtt
    25981 cttcaaagaa ttcagctaag gaagcatacg gcacaaattc tcctgcttcg tttagaaaca
    26041 gtgaacgcgc atagccgcca tatgcggcat caacatgtaa atagaaatag atcccttcat
    26101 cttttaacct ttctcttaat tggacgattt tatctacact gtctacttga ccttcttctg
    26161 tcgttcctac gacagcaacc acacctagaa ttgggatttt ttgtccagct aattcacgaa
    26221 ttgttttttc caaagcatta atatccattc gataatcctc ttgaactggg atagcaacca
    26281 tctgatctaa gccgactccg caaatatcta acgccttcat ccaagaatag tgttttgttt
    26341 gagggaccag ccatttccct aatctttgga tattttttcc gctacgggaa gaagctgctt
    26401 tgacctcatc catttcttcg tccgtaaagc tttcggtcat ttccaaaatt tcttcaacag
    26461 ataaattcaa aagttcccac tcagacattt ttttcacttt ttcaggcagc acttccttga
    26521 cagctaaagg aatcgatttg atgcaacggg cgtaccataa tccttcaaga ttagcaatag
    26581 aaccatctgc tgcaatgtgt ccccatccgt ctgtcatatt gaataatgaa gcaaaatcct
    26641 gtcctacttc tgcttccatt tgcgaagttg ccatagaaga ttccaatgca acattatttg
    26701 ggttccacag catcgcatag ttgtaagcta ataaagcagg catcaatgtt tcagcattca
    26761 tttgtcccca gtaacggcct gctgaatgcc atgggatcga tcctgcgcgt aatcgctgac
    26821 tgacttcatc caagacctct atcatatggt cgcgggtggc tagataatct ggagtgagct
    26881 tatctccttc gctaattgca ggcatatctg aaggaatata attttctctc catccaagat
    26941 gttcatctac catcttgttc aacaacattt tataaactgg tccattttca gctttgtcgc
    27001 ctataaagac ggccttgata tccatatctt tcatttttct tccctccata ttttgttggt
    27061 caatgtcttg cagatacatg tgtttatctg taacacctct tcactatagc gccaaaaatg
    27121 agaaattttt ttttataaca tcaaatccac gtagattttt acaaacaaaa ataaataaag
    27181 tcggattttt ctgataattc aacgcaaaaa agcgaacaat ttttcaataa atcagtcttc
    27241 taaaatagat aacctaaaaa aagcg
//
LOCUS       DACDTO010000030        25519 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDTO010000030 DACDTO010000000
VERSION     DACDTO010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.030"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            153..2213
                     /locus_tag="IWK70_001304"
     CDS             153..2213
                     /locus_tag="IWK70_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613867.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2296..2865
                     /locus_tag="IWK70_001305"
     CDS             2296..2865
                     /locus_tag="IWK70_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9613868.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3040..3846)
                     /locus_tag="IWK70_001306"
     CDS             complement(3040..3846)
                     /locus_tag="IWK70_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613869.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3984..5498
                     /locus_tag="IWK70_001307"
     CDS             3984..5498
                     /locus_tag="IWK70_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9613870.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5901..6884)
                     /locus_tag="IWK70_001308"
     CDS             complement(5901..6884)
                     /locus_tag="IWK70_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9613871.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6889..7536)
                     /locus_tag="IWK70_001309"
     CDS             complement(6889..7536)
                     /locus_tag="IWK70_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9613872.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7621..8253)
                     /locus_tag="IWK70_001310"
     CDS             complement(7621..8253)
                     /locus_tag="IWK70_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9613873.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8269..8820)
                     /gene="coaC"
                     /locus_tag="IWK70_001311"
     CDS             complement(8269..8820)
                     /gene="coaC"
                     /locus_tag="IWK70_001311"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9613874.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8817..9554)
                     /locus_tag="IWK70_001312"
     CDS             complement(8817..9554)
                     /locus_tag="IWK70_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9613875.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9551..10540)
                     /locus_tag="IWK70_001313"
     CDS             complement(9551..10540)
                     /locus_tag="IWK70_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9613876.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10798..11340
                     /locus_tag="IWK70_001314"
     CDS             10798..11340
                     /locus_tag="IWK70_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9613877.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11357..12442
                     /locus_tag="IWK70_001315"
     CDS             11357..12442
                     /locus_tag="IWK70_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613878.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12454..13260
                     /locus_tag="IWK70_001316"
     CDS             12454..13260
                     /locus_tag="IWK70_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613879.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13261..14088
                     /locus_tag="IWK70_001317"
     CDS             13261..14088
                     /locus_tag="IWK70_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9613880.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14085..15158
                     /locus_tag="IWK70_001318"
     CDS             14085..15158
                     /locus_tag="IWK70_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9613881.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15586..18282
                     /locus_tag="IWK70_001319"
     CDS             15586..18282
                     /locus_tag="IWK70_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9613882.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18359..18847
                     /locus_tag="IWK70_001320"
     CDS             18359..18847
                     /locus_tag="IWK70_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9613883.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18859..19353
                     /locus_tag="IWK70_001321"
     CDS             18859..19353
                     /locus_tag="IWK70_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9613884.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19374..20183
                     /locus_tag="IWK70_001322"
     CDS             19374..20183
                     /locus_tag="IWK70_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9613885.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20180..21001
                     /locus_tag="IWK70_001323"
     CDS             20180..21001
                     /locus_tag="IWK70_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9613886.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21052..23151
                     /locus_tag="IWK70_001324"
     CDS             21052..23151
                     /locus_tag="IWK70_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9613887.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23148..24266
                     /locus_tag="IWK70_001325"
     CDS             23148..24266
                     /locus_tag="IWK70_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9613888.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24305..25183
                     /locus_tag="IWK70_001326"
     CDS             24305..25183
                     /locus_tag="IWK70_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9613889.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25374..>25519
                     /locus_tag="IWK70_001327"
     CDS             25374..>25519
                     /locus_tag="IWK70_001327"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613890.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEF"
BASE COUNT         8060 a         4447 c         5404 g         7608 t
ORIGIN      
        1 aagttattat tccgattttc ttgaaacaat aaaaaatcaa aaaaaatact ctcctaatag
       61 tccatcttct tttgatttta tttatataat gaaatcatct tatgaaaatt actacttcat
      121 gtttagacaa gagtagggag gactaagaat aaatgtgttc aaaaagacgt tcaaaacaac
      181 taaataaaca cgcttgtcgt ttagataagg aacggtcgtg ttctgaaaaa cactacgtta
      241 agtttatgat gcgcacgatg ctgatcagtt tatctgtagg tgtctatatg catgtacccg
      301 ttcaggcaac gacacaaacc atcaaggtcg tacgtacctc tgtttctgag accactgatt
      361 cccctatcac tattactcat actttcactg atgcaaatgg aaaacagcta cttcctgcat
      421 tgacttccta taataaagtc agttatcctc caacgataaa ccactactgg ttaattccta
      481 acaaaacatt atggacatcg gaaaccggaa gcagtgtgcc gctttctatc ttttccgata
      541 tgactgatcc tacaaaaggt gatcagttaa aacagatcac tgatattttg aatcatgctt
      601 tgacagatat aaaggacggt ggaagcgcac aagtttctta tgtatatgaa aaagattatt
      661 cacagtttga aagaaagaaa atcgatgttc tggcagggac aaaagtttcg atatctgatc
      721 tggtagaaag gctgaagaac gcagatggaa cacaaggaga tatgtccaaa gtaatttcaa
      781 acaatgggaa tatcgtggat acgactaata caggaacatc tactatttcc ttgtcctatt
      841 ttgatccaat tgctttaaaa acaatgactg catctgctgt gatccatgtt cgtgctgatc
      901 aaacagctat caaaggaaaa gatcttcact taagaacagg agagagttac gcccctaaag
      961 atcttgttga atatgtaaca aaaagcgatg gaacttcagg aaataaagaa gaaatcaaaa
     1021 tgaacgggac agtagatact acaactccgg gagaatatcc cgttgtatta acttatatag
     1081 actcgaaaac atcaaatgtg gcagaaacaa cagctgtagt ttatgtagaa gcatctgctg
     1141 cttcatcgac tgtcatcact cattttatag atagtcaaac aaataaagaa cttcaagcag
     1201 aaacacatat gagtagtgta aatcgtattc ctgatttgcg cctacctgct gaattttctg
     1261 atcgagtgcc aaatatagat ttatcgactt atgaacaaaa tgggaaagtc acttctattt
     1321 ctgattggat gcaagcaaac ggattagaca gaaaaagtca ctggtcggag atccttgaag
     1381 tacttcactc agaactgatg aataatagga agcaatcagg aggggaaatt acctttactt
     1441 atgtttatat accagacaat cgttcattag aaggtcatga tcttattgtt catccaaacg
     1501 aaaaaataaa caaagaagag ttgatccaat cagcaacaaa ttccttaggt aaacccgtac
     1561 cgttaaagga tgtatcggtt acaatcaatg aagtaccatt ggaggatggt tatttgacca
     1621 aacaaacagg tgattttacg attatgtata gttatcatga cccgtattca ctccaagaac
     1681 tccatgctca gtcactatta catgttttac cattaaacaa acagatagat caaaaagaac
     1741 aaaaaagacg agaagaaagc ttgtcagaaa aaaaacaaca aatggctcat gcagtttctc
     1801 caacagggaa agtaaaacaa acaagaagcc tagagttagc aagaatatct agaaaaatct
     1861 cagaatctaa taaaacgata aggcaagaag ctcatttggc cccacaagtt tctggattgg
     1921 aaaatgaaat tttagagcct aaaataaaac aacaatcaga aaaagaattt gttgctaaac
     1981 agtcggaaga tcagaaggaa tcagatgacc agaaggaaac gaagaaaaaa cgcttgtcaa
     2041 ctattgattc caccgatgag catctgcagc cgcaagtacc acttcctagt ccgggaacac
     2101 ccgcacctgg tggaggtatt ccttcttctg ctttaggaga tttcatgcga ggtatggcag
     2161 gaacatgggt atacgctaat cgaaatgaat ttgacgcaga tgactggttc tgatttatat
     2221 ctctatctgt tatagtcaaa taagtagctg agtcatatgt tatttggcat aatggagacg
     2281 aggagggata gaaaaatgtt agataaaacg ttaccttacg cagaaatctg gatgacaaga
     2341 ccacttcaac aaaatgttcc aatcacgcct ttagcggaag gttttgaatt tcgattttat
     2401 caaaaagaag atgaatctgc ttgggcggag attgagactt ccgttgggga atttcaaacg
     2461 acagatgaag cacttgagta ttttcaacaa aactttgctc cttatcctga agaacttgaa
     2521 agaaggatgt tttttgtaga gacaaaaaaa ggagaaaaag tagcaacctg taccgcttgg
     2581 cgaaaaaaat caagagagca agtgtatccg gtttttcatt ggttagcagt gaaaccagat
     2641 taccaaggaa aagggctcgc aaaagcattg actgccagag tcttgcaaga atttcctacc
     2701 ctccatacgg acggaccaat ctatcttcac acacagacat ggagccatca ggcgattgct
     2761 ctttataaaa aaatgggttt tacttttatt gctgaaaatc tagacggcac acctaatcca
     2821 gattatgaaa aagttatgca ggttttaagt aaattagata aataacgaaa tggtgaaata
     2881 agtcctcagt attgggataa aaaacgaaat agtcattcct catatcttac ttttttgagt
     2941 taatactatc ggataactaa tggcatgcca tacattcaaa aaagaagggg cagtgacaaa
     3001 agtattgtca ctgccccttc tcttgttttt tagcatgttt tatcgtttga tttttttagg
     3061 aataaaattc ttgatgaacg taaatttttg gtaatcgttt cgactataga ggtaaccaaa
     3121 tacactgaaa acaactgctc caataaaatt aacaaataaa tccttcatag tatccatgat
     3181 tcccacgtct aagtaaccat tttggatgat tgttttatct gttttaccat ctgcattttg
     3241 actttcaatg attgtccgag tgatatctgg aacatgatag acagtgtttc cgtctttact
     3301 taactccaca ctattgattt gctggatgat cgtatctttt tgcatatcga attgcagtaa
     3361 tttatctgct gaaaattcca caaactccca cataacacca actgtcatcg aaaaacagaa
     3421 ggaaacaact gctagtaata aaggagtcaa gttcatggtt tttgtatttt tattgagcaa
     3481 ataaacaagg gaaaagccga cacccccagc taaaaatccg ctgaatgcat gtagaaaagt
     3541 atcccacatt gaaaaatagc cataaaaatc acttaactct cctaaaactg ttgaactaaa
     3601 aataaaagat aacaaaatga tttctagcat atttggaatc tgtatcttta atatcttttc
     3661 cagaaaataa gggatttcaa ataaaattaa tgtcagtaat aaaagcattg tatttcccca
     3721 gtgttggttc cagacctgat ccactagcat aataacgacg atgatacgta ataaaatata
     3781 gattccgttt gtgattctac ctgcagtttt tgtactgttc gtaaaaaagg ttttcaaatg
     3841 attcataaaa taactccttc atgtatatcc ttaattatac taagaagcgt gtgtttacca
     3901 aagctatatg acttaaagaa aaacagagtt gatattgtga aaaaaatcac ttaatatagt
     3961 ataaggagaa aagggggata caaatgaaaa aattagttgc tggtttatta acagccgttg
     4021 cacttcttgc tatgagcgca tgcggcgcag atcagaaaaa tacagcgcag acaagttcta
     4081 gtacgaagga atctgccgaa gtatcatcag gcgcttctga acaagtatat acagatccaa
     4141 aagaaatgaa agaatcttat gatgtcgtga ttattggagc tggtggtgca gggatggcag
     4201 cagctatcga agcaaaagat gctgggttga atccagtaat tttggaaaag atgcctgttg
     4261 ccggaggaaa cacatctaaa tcatcaagcg gtatgaatgc ttcagaaaca aaatttcaaa
     4321 aagaagaagg aatcaaagac tcgaatgatg ccttctatga agaaacgcta aaaggtggtg
     4381 gaggaacgaa tgataaagaa atgcttcgat actttgtcga tcactctgct gatgcggtcg
     4441 attggctaga tcaaaacggt atcaaactgg acaacttaac gatcactgga gggatgagtg
     4501 aaaagaggac acatcgtccg tctgatggct cggctattgg tggatactta gtggaggggt
     4561 tattgaataa cgtccatgag cgggaaatcc ctatttttgt caacgcagat gtgacagaca
     4621 ttaagaaaaa tggggaaacg gtcaatgaag tgactgtaca tgttcaagga gaagaaccaa
     4681 aagaggtcac cggaaaagca atcgtagtta ccacaggcgg ttttggtgcc agcaaagaat
     4741 ttattgaaaa atatcgtccc gatttagctg attatattac gacaaatcaa gaaggatcaa
     4801 ctggagatgg tatcaccatg attgaaaaat taggtggaca aaccgttgat atggataaaa
     4861 tacaaattca tccaactgtg cagcaggatg agggggtctt gattggtgag gttgttcgcg
     4921 gagaaggagc aatcttggtc gatcaagagg ggaaaagatt tgtcaatgaa atggatacaa
     4981 gggataaagt atctgcagca attacagcat tgcctgaaaa atcagcttat ttgatttttg
     5041 accaaggcgt gcgtgatcgt gccacagcta ttgaatttta tgaccaaaaa ggatatgtca
     5101 caactggaaa gacaatggaa gaattagcag aaaaaatcga tttaccaaaa gaaacactag
     5161 caaaaacagt tgatggatgg aatacggatg ttgcggagaa aaaagatgct caattcaacc
     5221 gtgccacagc catggaacat gacttatcgt ctccgaatta ttatgcgatc aaaatcgcac
     5281 caggaatcca ttacacgatg ggcggtgtga aaatcaatac gaacactgaa gtattagata
     5341 aagaaaacca gccaattagg gggttatatg ctgctggtga agtcacaggt ggcttacatg
     5401 gaaacaaccg gatcggtgga aattcagttg cggagatcat tatttttggg cgacaagcgg
     5461 gcatacaagc agctcgattt gcttcggaaa atgaatgaaa tagaacgtaa ataaaaagag
     5521 gttgtgaaat cagcgttttg agctaatacc gagggtcagc tgattgaaca ctgtttagtg
     5581 aagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaagt aaaaacagct
     5641 tcttatgttt ttaacttggt tggacttatt accgcagatg caagcttttg aacaccgttt
     5701 attcggaaaa agagcgtggg acaaaagtaa agaacacttt tgtctcacgc tctaaacacg
     5761 tataaacggc ggaagcagaa gaaattcctt cggaaataag ctgaaattca caaaaatttg
     5821 aaaagcaatt ttcgtgaatt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
     5881 cttttttgat cagcatgatt ttattcagtt atcttcaaaa tgatttttcc gatgtttttg
     5941 tttgcttcca tatattcatg cgcttgtttg actgcgctga attggaatgt acggtcaatg
     6001 attggccgaa tcttgccatt ttctagaaaa ggcccagctt tttgcataaa agtttgtgcg
     6061 agctctgctt tatactgatc acttctaggt gtcaataatg taccaagcag ttggacacgt
     6121 ttcccaatca atacaccaag gtctatctca ggcacgatgg tcccacctag cataccaatc
     6181 aagacccaac gtccatcaac ggctatagaa gctaaattct tttcccagta agaagcgccg
     6241 atgaaatcca aaataacatc gactccccga ccttcagtga tttttcgtac ctcttcttga
     6301 aagtcttgtt ttttatagtt gatcaattca tctgcaccaa gttctttaca aaaggcgagt
     6361 ttttcttctg atcctgcagt aacgatgatt ttcgcgtgag aaagctgtct agctaattgg
     6421 atcgctgctg ttcccacacc acttgctcct gcatggatca agacagtttc gccttcttgt
     6481 aattttccaa gccagtaaag tgtttgataa gcagtcaaaa aaacttctga gattccagca
     6541 gcagaaacat agtctagcgt atcagggaga aggattgcac gatcggcagg catcacagca
     6601 tattcggcat aaccacctag attgactaat ccgtatactt ttgtcccagg agtaaaagtg
     6661 gagatatcac ttttattctc aactacctct ccagctactt cgactcccaa aatagggtag
     6721 ggaggtttca aagagactga ttctcttttc ataatatctg ttcgattgac agctgctgta
     6781 tggactttga tcagtaattc gccttgtttt ggtttaggaa caggcacttc aatttgttct
     6841 aatacatcta cagaaccagg atgcttgatt gaaatggcac gcatggatct attcctcctt
     6901 tgcccattct tggaatgctg tattcaactc tttgctacct tcagaaagta ctagagtcgt
     6961 acgagaaata tccgtttctt ccactagagc ggcaagtaca gcagcaactg tagagcgagt
     7021 gatcgttttt gatgccttct cattaggctt agttaattga atggaagtaa cttcttcatc
     7081 gttagtcaat gtgactggtt gaagaatgac aaaatctaag ttagttcgtt tcagccattc
     7141 atctgcataa tgtttagtaa tataataatc tgtcatggac tcaggccatt ttgctggttc
     7201 atctgcataa acagcactga ccatcagata gcgagagaca tgcgcatttt cagctgcgat
     7261 cactgttttg attgcgccat ccaagtctac ttgcagaaga ttctttcctt gagagccggc
     7321 agcaaaaatc aatacatcga tgcctttgaa tgcttcggtc attttttcca cggaccaagt
     7381 taaatcaaaa gagacatacg aaacattctc tttttttact aatgtttggg aagcaacatc
     7441 tctcacccct gcaaaaattt catgggaact tggagctaaa tcttttatca ggtgtcggcc
     7501 aatttgtccg tttgcaccta caacaaaaat tttcatttgg atcatccttt cgacgtcaaa
     7561 tctttccatt caatagtaac accaaaagag aaaaaaatca gagatcggct acgatgacta
     7621 tcagttagta agagaagggt gcttgaagta tcttaacaag acattgcata ctgctgcagt
     7681 cgcaagacct gcgacgattc cttcaggtat accacttgta aaaataaccg ctagaatagc
     7741 tgaataaaca gcatttaaat ttgttccgat gactttggca taatcttgct ggaataaaaa
     7801 gtagatcata ttcatcacta aaatcgtatt ggtcaatgaa ccggaaagtc cagctaagaa
     7861 taaagaaacg gaatctgctt tttgtttcat tagtttgcgc agacctttat aaacgaaata
     7921 aggaaccacc ccaatcaaga tgcgaggaat aaacgcgatc agcaaggctt tccagcttcc
     7981 atgttctgtt ccgatgactg ggataaacgg agaaaagaca aaagacaaag gtgtttggat
     8041 aactgtgcta cggatcatgc tgatcagtcc aaatgtaccg ccaagaaaag cacctagtct
     8101 tggtcctaaa acgatcgaag caatgattac aggaatatgc atggttgttg cattgattgg
     8161 accgataggg ataaaaccta aaggcgtaac agctaataaa atcaaaatag ctaaaaacat
     8221 cgcagttaat gtaaagtttt ttgtattttt cataagtact ccttttttct aagcatttct
     8281 ttcattaaga gtcagcatga cctgttcgat gatcgtctga atatcagcta aggcaccttt
     8341 tccaaaatct ccacaagcta aaagagattc tcttggttca atttcctgat agccgacaga
     8401 tctcaatgtt tccagattac gctgagtgat cgggttttga tacatattcg tgttcatggc
     8461 aggggcaatc aattttggtg ttcccactgg aagcgccatt gcgactgttg aaagaagatc
     8521 gtctgcaatg ccgtttgcta gcttgccgat aatgtttgct gtagcaggcg cgatgagaaa
     8581 caagtcagct tgttttgcta gttcgatatg attgattacc tgcggatttg attcttgcat
     8641 gacgtctgta tggactgcgc gcttcgacag agattgcaag gttaatggcg tgataaaatg
     8701 tgtgctgctt ttagacataa tcacatctac ctgggcacct aatttgacga attgacttgt
     8761 aagatcagcg cttttgtaag cagaaatact tccagtaacg cctagtaaga tctttttcat
     8821 tttgattcct ccagtctaat tttctcagta atcaatgcag caatctcaga tttactttga
     8881 gcttcttcca ctgttccatc tttagataag agatagccgt ggtgtgtctc gccatggatt
     8941 tccgtcaaat catttgcgaa aataaaatca gttttgttat ttatcagact attttttgcg
     9001 actgccagaa gagattcctt tggtacatct actaatagtt tgaaaccaat gatttttgct
     9061 tttggctgcc attcacgtag ataagaaatg atttttggtg ttttcttcaa aacgagcaca
     9121 agatgctcgg tgttagaaga gatctttgaa acagtggtat ctgtctgttc attttccgca
     9181 aaccaattgt ccagttcttc ttgcgttgaa gaagtgggta ggttttttgc cagctgttct
     9241 tctgaaaaac taaaagcagg agtaaaatca ctgactgcca tactgtggat aatcgcatcg
     9301 tagtgttctt ttttcgttag ttcttctaat tgtaaaaata agtccagtgc agattcgatc
     9361 gtataaatcg ttatattgga gtgtcttttc ggtttcagtg cttgtttcgt agtcacgtaa
     9421 tcgattgtgg tatccggatt ggcagctaaa taatcagcta atgcttgccc taatcgtcct
     9481 gtcgaatgat ttgtaatagc acgtacttga tcgatttttt cagtcgttcc tccagctgtg
     9541 attagaatct tcatttatgg tcacctacat ttactagtgt tcttccttgg tgtgttccag
     9601 caagtagttt agatgctgtt tcaggcaatt gatccagagt aatctcttgg acgaccagtt
     9661 catctgcaat ctgcaaatcc gccatctgtt gccataaaga aagacgttga tctattggta
     9721 cattgacaga atcaattcca atcaactgga tgtttcgcag aataaaaggc aagacagtcg
     9781 cgtttagttt gattccccca gcattgccac ataaagctac tgcaccgtta taagagatca
     9841 atggaagaag actgcttagc tgctcgccac ctactgtgtc gatgacatag tcgatttgct
     9901 gttttcctaa agcttttgtt gtctctggca gaaattcatc aggtcgtacg acttgcaaag
     9961 cacctagact ttttagccat gtgacagact cttcttttct ggaaaaggca atgattgact
    10021 ggaatcctag tttatgtaac agggctatcg ctgtactgcc tacaccgcca gaagcaccag
    10081 taacgacaac ttttttatcg gaggtcatac cttgacggat taaagcgttc acacacaaca
    10141 tagcagtaaa ccctgctgta ccaaggatca tcgcttgacg cagcgtcata ttttttggta
    10201 gaggaactaa ccagtctcct ggtacttttt gatattggct ataaccgcct ggatgggtga
    10261 cgccaagccc aaaaccagta gcaagtacaa gatcttcttt tgaaaaacgt ggattgttac
    10321 tttggacgac agttccggat aaatcaatcc ctggagtcat cggatatgtt cgtatcacct
    10381 gaccagactc gctcattgcc aatgcatctt tataattgac atcagaatag gcaattttta
    10441 ccaagacttc attttcagcc aactcagtaa atggcagatc actgattgct gtatggaaaa
    10501 caggttcctt tattacctga aaagatcgaa aagcgttcat tcgtgagaca ctccttttaa
    10561 aattttcttt tatcatacca taaagcctac tcttttattc ttgttcttcc gtaaatttag
    10621 acaaaaatta ggtgaaaaat aaagaatcaa acaactagaa tttattccaa aagataaaga
    10681 aatattcagc aaaaaaaatt ttacaaaggg ttgccttttt caataaattt cgtatactaa
    10741 tacgagtggt agtaaacagg aagtttacta ttagaaaact tcgattcgga ggaaattatg
    10801 gaaattggtg agaagttaag aaatttgcgc atccaaaaga atttgacaca agaagaacta
    10861 ggcgaacgca cagatttgtc aaaagggtat atttcccagt tggaacgaga tttaagttca
    10921 ccatcaatgg aaacattctt ttctattctt gaagtcttag gtgtgactcc agaagaattt
    10981 tttcatcaag aagcagcgaa tctgcaagtg gtttattcaa aagaagatca tacggtttat
    11041 ttcgatgaag aaaacgggta tgaattggaa tggctcgtgg ctgattcgaa tgaaaaagaa
    11101 atggagcctg tcttgctgac ttttgaaggt gcaggagaat ataagacgtt tgaaccttca
    11161 ttatctgaaa cgtttatttt tgtattagaa ggggaattag agttaagctt aggagagacg
    11221 gtctatgtgg caaaagcagg acagtcgatc tattatcaag cgacaaaaca acatcaatta
    11281 aggaatcatc tcaaaaaacg aacaaaagta ttgattgttg caacgcaatc atatttgtaa
    11341 tatagggggt aaaaacgtgg ggaaaccaat catttcattt gatcaagtag tgaaacgcta
    11401 tgatgatgaa acagtattaa aaaaagtaag ttttgagatc gaacaaggaa aattttatac
    11461 actgcttggt ccttccggct gtggtaaaac aacgatccta cgtattatag ctggattcac
    11521 cgaagctaca gaaggggaca tctattttga agggaaacgg ttgaatgatt tgccagctaa
    11581 taagcgacaa gtaaatacag tcttccaaga ttatgcactt tttcctcata tgaatgtttt
    11641 tgataacgtt gcttttggat tgaagatcaa gaaaatgtcc aaatcagata tcgaaaagaa
    11701 ggtaaaagat gctttgcgta tggtccagct tcctggatac gaaaaacggg agattagtga
    11761 aatgtctggt ggtcaaagac agcgtgtagc gattgcacga gcaattgtaa atgaaccgaa
    11821 agtattgcta ttagatgaac cgttatcggc gcttgatttg aaactacgta cagatatgca
    11881 gtatgaactg cgggatctgc aacaacgatt aggaatcacg tttatttttg ttactcatga
    11941 ccaagaagaa gcgttggcaa tgagtgatga gatttttgtc atgaataaag ggaaaatcgt
    12001 ccaaagtggt acacccgtag atatttatga tgagccaatc aatcatttcg ttgctgattt
    12061 tgttggggag agcaatatcg tggacggtgt aatgatcgaa gataatctag ttgagtttgt
    12121 tggtaagcaa tttgaatgtg tcgatggcgg gatgcgtcta aatgaaccag ttgaagttgt
    12181 tttacgccca gaggatttga cgatcacgac accagataaa ggaaaactag tcgtaacggt
    12241 cgatactcag ctgtttcgtg gtgtccatta tgaaatcatc tgttatgatg aacagcaaaa
    12301 tgaatggatg gtccattcaa cgaaaaaagc taaagaagga agcaaagtag gattggcctt
    12361 tgagccagaa gatatccatg tcatgcgttt caacgagtct gaagaagaat tcgatgcacg
    12421 tcttgacagc tacgaagaat aggggggaca agaatgaaaa caatgcgcag gatctattcc
    12481 gtcccttata ttttgtggct cggtttattc gtgatcgcgc ctgtattaat gatcatttat
    12541 caatcttttt ttgatatgaa tgggcaattc acgctgaata attatctatc ttattttacg
    12601 tcggggacat cgctttctat gacgatcaat tctgtatggt atgcattttt gatcacgttt
    12661 ttcacgctga tcatcagtta cccgactgct tattttttaa caaaactgaa gcacaagcaa
    12721 ttatggctga tgctgattat tttgcctaca tgggtgaatc tgctgcttaa agcttacgca
    12781 tttattggga ttttcagtgt gcacggcagc gtgaatcatt tctttgattt tatcgggatc
    12841 gggccgaaac agatattatt cacagacttc agttttctgt tcgtggcaac gtatatcgag
    12901 attccattta tgattatgcc aattttcaat gcattggaag agatgaatcc ttctttgatc
    12961 agtgctagta gagatttagg cgcgaatagc tttgagacct tccgacgagt gatttttccg
    13021 ttatcattaa atggtgtgaa aagtggtgtc caagcagtat ttattccttc tctttcttta
    13081 ttcatgctga ctcgtctgat tggcggaaat cgggtgatca ctttaggaac ggctatcgaa
    13141 cagcatttct tggttacaca aaactggggg atgggttcta caatcggtgt gattttaatc
    13201 attgctatgt tcatcgtgat gctcctgaca ggcgagaaga aaaaaggagg tcgctcataa
    13261 atgaaaaaat ttaaatggtc gtatctttat ttagggatcg tctttttatt cttatatgca
    13321 ccgatcttct atctgatttt ttattccttc aatgaaggag gaacgatgaa cagctttaca
    13381 ggatttactt gggaaaatta tgaagctgtt tttgaagata cacgtttgat cattatcgta
    13441 ttgaatacgt tcatgctggc tttcttatct gcattgatcg ccacgatcat cgggacattt
    13501 ggggctatgg gaatctatta cacgaaaaaa cgacgtgcac ggacagcgtt gttgagcatg
    13561 aataacatct tattagtctc tcctgatgtt attatcggtg ctagtttctt gattttcttt
    13621 acactactag gattcggatt aggttttcag agtgtattgc tttcccatat tgctttcagc
    13681 attccaatcg tcgtcttgat ggtcttgcca aaattgcagg aaatgaacga ttcaatggtc
    13741 gatgcggcta gagaccttgg tgctaataat ttccaagtag tcaaaaatat cattttgcct
    13801 tatctttctc caggaatcat agctggatat tttatggcat ttacctattc tcttgatgat
    13861 tttgcggtta catttttcgt cacaggaaat ggctttacga cgctctcagt agagatctat
    13921 tctcgtgcaa gacaaggaat cagtttagaa atcaatgcgc taagtgcgtt agttttcttg
    13981 ttctccatga tattggtcat tggatactac tttattagta aagaaaaccg tccaaaacgg
    14041 ctgaagaaaa acagaagagt acgcgagggg gtggcacggc tgcgatgaaa aaattacaat
    14101 ctctgttttt aggtattctc atcattattg cggtgctgct gcttggtgtt cgtcaacttg
    14161 aaaaaacaag tggcatgagt ggagcaaagg tacttaccat ctataactgg ggagattata
    14221 tcgatcctag tttgatcacg aaatttgaaa aagaatatgg atacaaagtg aattatgaaa
    14281 catttgattc taatgaagcg atgttcacaa aaatccagca aggtgggaca aactacgaca
    14341 tcgctatacc aagtgaatat atgatccaaa aaatgatcaa agaaaaacta gtacttccat
    14401 tagatcattc gaagatcaaa ggattaaata atattgatcc acgattttta gacttggatt
    14461 ttgatccaga taatcagtat tcgattccat atttttgggg aactttaggg atcgtatata
    14521 acgataaatt ctttgatgca aatcaaatta agcattggga tgatttgtgg aagccggaat
    14581 taaaagacag tttgatgctg attgacggtg cacgagaagt tatggggctt tctttgaata
    14641 gtttaggcta ttctttgaat agtaagaata tggcacagtt gacagaagca gcaaataaat
    14701 tgaatcattt aacaccaaat gtcaaagcaa tcgtggcaga tgagatcaaa atgtatatga
    14761 tcaatgaaga agcctccgtg gcagtgacat tttctggcga agctgccgat atgatgtggg
    14821 aaaatgaaca tttacattat gtgatcccac cagaaggatc caatctttgg tttgataata
    14881 tcgttatgcc aaaaaccgtg aaaaataaag aaggtgccta tgatttcatc aactttatgc
    14941 tagaaccgga aaatgcggca caaaatgcag agtatattgg ttattctaca ccaaatgaag
    15001 aagcaaaagc gttattgcca aaagacattt cgtcggacga acaattctat ccatctgatg
    15061 acacgatctc ccatcttgaa gtctacaaag atctaggatc gaaataccta ggcatctata
    15121 atgacctatt cttagaattc aagatgtata ggagataatg gcaaaactca gtaagaatgg
    15181 ggacttatca acgttctaaa gagctaaaaa tcgtcctttt tctatctgag ttttccaatc
    15241 ttctaccaaa agcggatgaa acgccgcttt ttttgtgtta ttttattttt tctaccacgt
    15301 ttttctacca gagaaaagtg gacaaaacca ttgtaaaata gggcccgaga cgggctagta
    15361 aatctaagat tgaaaaagtt acagaaggat aaagtaattg gaaactatgc aaatatactt
    15421 aacccttctg taatctatta ataaaactaa tatatgaatc cagttcaata tttgtagcta
    15481 tgaaaaaaca taaaaatcat attagataaa aactaaattg gtagataaat tattttgtca
    15541 gatatctgag gcaaatattc gactatttgt gtttcaaagt aaaaattgga atacaaatat
    15601 agaatagaca tattgcagcc gatagtttat tatgctttta gacacattaa actggaggaa
    15661 aaaatgaaaa agagcgctaa agatcgagtt tttgatgagc tgatacgact aacagggaat
    15721 atgtcttctg tcactgccga gaaaatttcg aagaatctag gtatctctcg tcagaatgcc
    15781 agccattatt tgacgagatt agtggaagat aaaaaggtcg agaaattacc gggaaagcct
    15841 gttttgtgga agcctttaga tgaatatgcg gttgtagata atacggagca aataaacgaa
    15901 gcctttcatt ctgtagttgg tcatgacgga agtctgagag aggtcattca aaaatgtata
    15961 gccgctgtca aatatccgcc taacggactc agtgtattaa taaatggagc cacgggtgtt
    16021 ggaaaaagtt ttttagccac aaaaattttt gaatacgcta tccatgagca aattattgaa
    16081 aaagatgcac catttgctat cttaaactgt gccgattatg cggacaatcc cgaactgctt
    16141 tcggcaacac tgtttggata caagaagggg gcctttacgg gggctgaaaa ggataccgat
    16201 ggtttattgg caacggccaa caacggctat ctttttctcg atgaggtcca tcgcctatcg
    16261 aaagaaaatc aagaaaagct ctttctattt attgatactg gaaactatcg tccagtcggt
    16321 gaaaatgtaa attggcattc tgctaaggtt cgttttattt tcgccacgac ggaaaaaggt
    16381 gaaaattatt tattagacac ctttgatcgg cgtattcaaa ttagtgttat gttgccaact
    16441 tttgaagatc gccccattcg agaacgatta gagctgattc aattattttt tcaaaatgaa
    16501 gcggatgttt tacaaaaaga tattattgtt tcaagagaag ccttgtcttt aatgttatct
    16561 catcccttta gtgggaatat tggtaagctg aagaatatca ttaaaatcag ttgtgcggat
    16621 gtgtatagtc gaacacaaga agaaccatta acgattggga aaaatgagat cttgattcag
    16681 ttaaatatgc tggaatccga agtagaaagc ttgccgattg ctagtttatt gataaagaaa
    16741 aatcaaaaga taaaaacgga tcgaatcaaa ccgtttgatt atgattcgat tttggaaaaa
    16801 attactcagc tgataaatga aggtaatttc aatgaaaatt tttcttcgat aaagaatgaa
    16861 gtacagcatt tggtgcatca cattgataag gattcttttt tatcaatgag tagaaaattg
    16921 atgattcaga tgtttagaaa ggtctgggta tccgttttag gaaaaacgta cggattgacg
    16981 acgagtggac ctgttgcaga aattattagc agatcctatg tcatttcccc aagctataag
    17041 acagacttaa ccgggataaa tcgtacatta gctgcaaatc tacctcggac gacttactta
    17101 tgtaatcagt tcttggagca tttaccaccg ctctcagaaa aggacgagaa ctttctaaga
    17161 ctgcttattg tcgtgacttt atctgattat gtagatgaat ccatcgagct gaagggcctg
    17221 ttgcttgctc acggagatag tacggctagc agcattcaag cagtagtgaa tcaattatgc
    17281 ggcaattatg tttttgaagc gttggatatg ccagtagaga gtaatgtgtc agagattgtc
    17341 gaatcgacaa ggaatttcgt gaaacggcaa gtgaatacag atagtctagt gctgatcgta
    17401 gatatgggct ctttaaatca aatttacaca cagatcaaga acgatttaaa aggggagtta
    17461 cttcttctga ataatttgac gacttccatt gcacttgata ttggcttgaa gatgacgatg
    17521 aatgcgccat ttaaacagat tgcggaggat gcagcagaca agtattcaat caatgttcaa
    17581 tactttgaag gcttttctca aagtagcaac attatcattt cctgtatgtc gggtttgggg
    17641 atttcggaca aattgaaaga tgtttttcgt catcatttat cggatgaatc catcgaagtt
    17701 ttcacgaagg actacagaga actacgtgaa ttaatcgacc aaaatgatga aacttatttt
    17761 gacaagacaa aattggtcat cacgacttcg gatcttccac gttccttttc aattcctaat
    17821 attaatgttt atgacatctt agacggtgat ggagcagcca atttggagca tattttgagt
    17881 cctgaaatga agccggcaga atttaaccgc ttgattcagg agctagtaaa attcttttcg
    17941 ttggaaggaa ttgcggaccg tttgaacttt ctgaacccaa cgatcgtgat gagtgaagtg
    18001 gaaacagtca tttctaaata tgaaaattac tatcatttca ctttaaatgg ccgtgtaaaa
    18061 ctaaatctct atatgcatac ggcattgatg atggaacgtt tattcttggc tcggacagaa
    18121 aaatctgaag aaccgaatta tgatttgaaa ccagatgaag atgaatttta ttatgtttct
    18181 agaagtattt ttcaatcaat ggagatgaaa tataatttta aagtcaatgc ttatgagctt
    18241 tcgctgctct atgaattatt aggaccgtat attcaaaaat aaagtgtcta aaaaagtgtc
    18301 taatctgttt tttttaggtt agacactttt tttaatgtga attaacgtga ttaaaccaat
    18361 gtttttaaaa gttggcacag gaattgctta tataaaagtg aacagaaaga aaggaattaa
    18421 ccatatgaag aaaattctaa tagcgactca cggtcactta gcttcaggct ttcttagcag
    18481 tattcaatta ctgacgggga aggttcaaga gattacggtg attaatgctt atgtggatga
    18541 gtgtgatttt gaacaggagt tgaatgtatt tgtaaaccag gtgaacgagc aggatcagat
    18601 ttttgttttt accgatttat ttggcggaag tgtcaatcag aagattacca aaaccttttt
    18661 ggagaaagag attgctgtaa ctattttgtc tggctttaat ctgccaattc tgctcgaaat
    18721 agtattggca accaaggaat taacaaagga ggatattcag gaactggtaa ataaatgcca
    18781 acaggaatta aagatgacca ttcttgatga ggtacatgta gtaaatgaag aggaagattt
    18841 tttttaggag gaagaaaaat gattaaagca gtgcgaattg atgaacgatt aattcatgga
    18901 caagtagcga tgacttggac aaagagtcta aatttaaccg ggttagtagt agcaagcgat
    18961 gaggcggcta ccaacgatat tcaaaagatg acattgaaga tggctgcacc atcgaatgtc
    19021 aaagtcatta ttaaaacagt tgatgacgcg attcatttgt taaaagatcc gcgggcgaag
    19081 gacatgcgct tgatggtttt agtccctaat gtcaaagatg cggtaaaagt tgcgaaagct
    19141 ttcccaacgg aaatcgaatt aatgaacgtg gggaatgctg ggaaaatgac accgccatcg
    19201 gaagataaga aaaccttatc aaaggaagtg atgttaacga cgtcagaaat tcaagactta
    19261 aaagaattag ttgaacttta tccagatacc gtttttcagc ctacaccggc gatggaaaaa
    19321 cgttcagcta tctctatttt gagtgatttt tagaaaaagg aaggagaaaa attatgtatg
    19381 atgcatttgt agtcgctttg gccgttttta ttggaattgc tggacatgaa atgttcggga
    19441 tgtccatgct gagtcgtccg attgttgttg ctccgttagc aggagcgttg atgggggatt
    19501 tgcagacggg gttgattgtc ggggcgtcac tagaagccat ctttatgggt gtcgtcaata
    19561 tcggaatctc tagtacggcg gagccttctt tggctgctgg gctggctaca gcgttcgcaa
    19621 tcaaaatggg aggaaacgtg gatgcgatta ttcccattgt ctttccacta gcagtattag
    19681 gattacagtt aatgaatatg attttttctt ttgtatgtgg accgatggct gctaaatttt
    19741 tcaaatttgc aaaatctggt gatcaaaaag gaatcatcaa tctgcacttc tttatgtggt
    19801 ttgtccacta tgcgctttat gctcttattc cattctttgc cgttttattt ggagcagatg
    19861 ttgtcaaaag cgtcttggat aatattccag aggtcatcat gaacggatta acagtcgctg
    19921 gaaatctgct gccggcagtt gggatggcga tgctgttacg gatgctttgg agtaaaaata
    19981 ttgctgtgtg gtttttcttt ggggccgttg ttatggcgta tctcgagtta ccattgattg
    20041 caattgccgc agttggaacg attattgcaa ttgttatagc ccaaagagat ctacaactaa
    20101 aaaatatatc tgttcatgca gttcaaagcg atgctgctgt aaatgcaacg cttgaccaag
    20161 aagaggagga tttctttcaa tgaaattagc tgatggaatt tctaaagatg agaaaaaatt
    20221 ggttcggaag atgttttggc gctcgatgac tatgtatgct tcggtgaatc cggtgacgat
    20281 gggcggcggt ggtttcgcct attctatgat gcccttcatt aatcatttct acaaaagtga
    20341 agaagatcga gctgcggcgc tggaacgtca tacacagtat ttttcgacga ccattccttg
    20401 cgcgtccttt gtcatgggga ttgcggcatc gatggagaag gaaaattctg aaaaagctgg
    20461 ttttgacgtg gaaagtatca acgcgatcaa aacgagtttg atgggaccat tggctggaat
    20521 tggcgattca ttgttctggg gagtttggcg tgtcgttacc gcgggactag cgatcaactt
    20581 agccaatgcc ggaaatattt tggcaccaat tatcttttta gttatgttca atatacctaa
    20641 ttacttgatg cgatactatg gcggcgtttt aggctattca ttaggctcta aatatattga
    20701 aaagctgtat gcgaacgggt taattgaaat tctgacaaaa gctgctagta tcttgggtct
    20761 gatcatggta ggtgcaatga cgatcagtat ggtcaaattc aataccgttt tgagcttttc
    20821 aatgaagggc gaagaactaa tgaatgtcca agaaatcctc gatagtattt ttaaaggaat
    20881 tgtaccgtta ttggtaacat tgagctgttt caaattacta gataaaaaag tcggcgtcat
    20941 caaaattatt tttggaatgg tcgtcttggg aattttattg tcattacttg gaatcgcata
    21001 gtaggaagct gcagctacta ggctgcagtt tttttagcaa ggagggaaaa gatgactatt
    21061 aatgaagtaa tttattcacc caaacaccca ttttttaatg ctgagttaaa cgacggaaat
    21121 gtgactactt caaacgaata cagctactat cggaatattc ctgatgtaaa gggattgacc
    21181 ttcgatgtaa gtaatcaaaa gacattagca aaccttgatg tttgcgggac gattcaaacg
    21241 ttgacctttt atcgagaaaa tttactaact gaggaaaaac caggtgtttg ggtcaataaa
    21301 cagctctctc aaaccaattc gttatcctta aaggttgaag taaacgggat tgtctaccat
    21361 ttgtcagaat cggatcatcg tattgaagtc gatgtgattc aagactgttt gcctagatat
    21421 attcatcatt attcgacgtt taaggtcatt gtagtatcct ttgctcccat actggctaat
    21481 aagcgtttaa gtatgcttgt tcaacaggtt tacattgtga atgaaacaca cgaaagcctt
    21541 caagtttcag tgaaagatgt gccttcgtat caagagaagt attcggatca gcaaaatgtg
    21601 ttgatttctc aaaaaggaaa taaggacacg atcgcacaac aggaagtggc aagcttcgcc
    21661 attgcttttg tggacccaaa tgcttttgaa gaagtaatga cttttcagga atctgatatc
    21721 gaaaaatggc tgaaggaaac attgaactat tttgctcagc tatttgggca gctgtcaatg
    21781 ccaaacgata gagttgtcca tctctatcgt cgtgcacttt atcaaagctt ttctagtttc
    21841 ggtatgaatc gacagggaca ggtcgtcggc agcaattggg gcagctatcc tgctactaat
    21901 cgaatttgga ataaggatat gtattacgca tcattgccat tcaccatgtt tgaaccggag
    21961 ttatgtcaaa aaacggttct atggtttgat caatatggcg ttaagtttcc tggttcaaaa
    22021 tttacaggag gtatcaatca ttctctgagt aattcgttat ccagtgcgtt actggctagt
    22081 ctttattttg aacatactgg ggatacaaaa ttctttgagc ggtgtccgca ggttctactg
    22141 aatgcttcaa gaattattga ggacatatta gctcaacgtc ggtcagggga accaatgctt
    22201 tttccatctg tttggttatc agacgccttt gcacttggaa agtatcatac cggcagcaat
    22261 ctttgtcttt ggaaggcctg tattggactt gcagaaattt ttgaagccct agaacaactg
    22321 agtcttagca agcgatacaa aaacattgcc tgtaaactaa aggaatcaat ttgtaaacaa
    22381 atgactatcg atggtccttt tggtgagcaa tttttggagg gaatcggtga tgaggagaaa
    22441 acaacctatt cagtgaagca ctatcaaaaa ccgattcttg agcaagggtt aatcttttta
    22501 agtgatgtga ttgtggatgg aaaaattaat ttattgatgc atgatggaga agaatcagac
    22561 actacgttga tgccttttta tcaattttta gataataaag atcatttata tcagaacacc
    22621 atggcctttt ctgctagttc tttcaatcca acctatagcg aggaaatcaa aggcatcact
    22681 tggggattag aatccggcgc cacttttcct ggctttatca ctgttttaat gagcgagctg
    22741 cacaaccctc aagctttcgt aacccgatta gaagaactaa tttgtttggc agatttagac
    22801 ggttcgtggt ggtggtggcc gtatcgctta gaggctcaac aaggagaagt cgtgcgagac
    22861 tttggctgcg gcaaatgcgg ctgggcatcg ggtctttttg tcagcctaat gatcagtcaa
    22921 tatttcggat tgaaattcaa aaaaggagta ttgcaaattg atcctgttga taatttaacg
    22981 ttttcttgga agaatttgaa gttcggtcaa gccttcattt cgattgaatg tacgcaatcc
    23041 gagttgagta tttcaaattt gtcagaaaag ccattgaaaa taagtgatac gaaaaaaatt
    23101 cttcacgtag aaaagggaaa aacgattcac attcaaagga gaaaaagatg agtatactaa
    23161 aaaatattac ccaatttaaa aaaatccaga gtcgagcaat cactcccgaa aatccaacag
    23221 gcgagaaagg taaagctgcg atgttgccaa gcgagctagg accaagccgc aaaggtcgtg
    23281 gcgcaattcc gctgacttct ggtgaagaaa ctgtgattgc ggatattact ggaacgggaa
    23341 taattcgtca tatgtggatg acgattcgtg atcgaacaga atttggcagt tttgttttac
    23401 gtgatttaat cttaagaatt tattgggatg ggtcggatag ccctgctgtt gaaagtcctt
    23461 taggggattt cttttgtaat gggtttggag agcgttgcga ggtgaactcg ttgccgatag
    23521 tggttaatcc tacagggggc tttaattcct atttcgaaat gcctttccaa acatccgcta
    23581 aaattacgat cgagaattta catccgaagg atatgtcctc atttttttat acgataaatt
    23641 acgctttggt ggacgaattg gaagaaaata gcctatattt tcacgcaaat tggcatcggg
    23701 aacgcattac caaattacag gaggattata agttaattga tcgattaaag ggtcaaggct
    23761 attacgtggg tacttacctt gcgcttacct gcctagaacg ttattggtgg ggcgaaggcg
    23821 agtttaaatt ctacattgat ggggatagtg agtatccgac cgtaacctca acaggttcag
    23881 aagattattt tggcggggcg tgggcctttc atgagtacga tgagttcgga cgtccacatg
    23941 cgaaaaacta ttcaacagcc tttttagggt atccttacca atcaaataga gatgccacaa
    24001 gagatcgttt tgaaacggga aaattgaatg ccgttcatgc ctttggtgac gacgcaatgc
    24061 cacgacatgg tctttatcgt tggcatcttc aagaccccat ctgttttacc gaagatttat
    24121 cggtcacttt acaacaaatc ggcaacgatg atgttcgctt atttgagcgg caagacgatg
    24181 ttgctagtgt cgcttactgg tattcaacag aagcttcagg aaatactaaa cgtcatttca
    24241 ctgatcgaga acgcttgccg cgttaatgaa gtgtttttca catattcgaa gggaggaacc
    24301 tatgatgata tatggggcaa ttgaagctgg agggactaaa tttgtctgtg cagtcggtac
    24361 tgaggaatta acgattttgg aaagagtcag ttttcccaca acaactccag ttgaaacaat
    24421 gaagcaggtc attgattttt ttaaaccttt taaggaacaa ttggcaggca tcggggtcgg
    24481 ctgctttggt ccaattgata ttcaatgcaa atcaacgagt ttcggtcata tcacatcaac
    24541 accaaaactt gcttggcaaa atttcaattt tgtaggaacg ctaaagcaat atttcaatat
    24601 tccgattgaa tggacaacag atgtcaatgc ggcttgctat ggtgaatatg ttgctggttc
    24661 tggcaaaggg ttagcaggtg ttgcatactt cactgtggga acaggtatcg gaggtggtgc
    24721 cttgttcaat gggactttcg tggaagggtt cagtcatccg gaaatggggc atatgttgat
    24781 caagcgacat ccaaaggaca atttttcagg aaactgtccc ttccaccaag attgtttaga
    24841 ggggatggca gcaggaccag cgattgaaaa acggttgggt gtaaaaggcc agaatttatt
    24901 ggctgacgat tcgttttggc agattgaagc cttttacctt gcccaatgtg catacaatac
    24961 aacactgatg ttttcgccag atagaattat ttttggcggt ggtgtaatga aacaagaaca
    25021 catgaagaaa aaagttcagg ataaatttgt tgagcttata aatggatatg ttgaaatacc
    25081 accaattgat agctacatta ttacgcccga attaggtgat aatgccggaa taatcggcgg
    25141 attagcattg gccagaaaag cggtaagaaa taagcaacca taattaatga atgtttcatt
    25201 gatactcatg tcaaaagtat ttaaccgagt aatcaaatga tcaaatgaag ttggataaag
    25261 cgaaaatgaa ttcaaataaa aaggctctaa gtcaaatcgt gttgttttca ttaaatcact
    25321 gaaaggaaat gcttctgcgt ttcctttttt ctttttgttg tgcgctgtat accttgagga
    25381 tattcactgg tggcgtacac ttaaatagta atatattgtg ttactttatg tctgtggtaa
    25441 tagttatagt tgcttaccaa tgtatgttca tttttcgaaa tgttgacttc ataatccttg
    25501 gcttaatgtt tgaattttt
//
LOCUS       DACDTO010000031        25495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDTO010000031 DACDTO010000000
VERSION     DACDTO010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.031"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(170..3319)
                     /locus_tag="IWK70_001328"
     CDS             complement(170..3319)
                     /locus_tag="IWK70_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9613891.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3333..4550)
                     /locus_tag="IWK70_001329"
     CDS             complement(3333..4550)
                     /locus_tag="IWK70_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9613892.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4540..6135)
                     /locus_tag="IWK70_001330"
     CDS             complement(4540..6135)
                     /locus_tag="IWK70_001330"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9613893.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6260..7045
                     /locus_tag="IWK70_001331"
     CDS             6260..7045
                     /locus_tag="IWK70_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9613894.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
     gene            7754..8113
                     /locus_tag="IWK70_001332"
                     /pseudo
     CDS             7754..8113
                     /locus_tag="IWK70_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(8408..9541)
                     /locus_tag="IWK70_001333"
     CDS             complement(8408..9541)
                     /locus_tag="IWK70_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9613895.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(9532..10200)
                     /locus_tag="IWK70_001334"
     CDS             complement(9532..10200)
                     /locus_tag="IWK70_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9613896.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(10656..11642)
                     /gene="lacD"
                     /locus_tag="IWK70_001335"
     CDS             complement(10656..11642)
                     /gene="lacD"
                     /locus_tag="IWK70_001335"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9613897.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(11655..12581)
                     /gene="lacC"
                     /locus_tag="IWK70_001336"
     CDS             complement(11655..12581)
                     /gene="lacC"
                     /locus_tag="IWK70_001336"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9613898.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(12594..13109)
                     /gene="lacB"
                     /locus_tag="IWK70_001337"
     CDS             complement(12594..13109)
                     /gene="lacB"
                     /locus_tag="IWK70_001337"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9613899.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(13128..13556)
                     /gene="lacA"
                     /locus_tag="IWK70_001338"
     CDS             complement(13128..13556)
                     /gene="lacA"
                     /locus_tag="IWK70_001338"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9613900.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(13576..14349)
                     /locus_tag="IWK70_001339"
     CDS             complement(13576..14349)
                     /locus_tag="IWK70_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9613901.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            14514..14993
                     /locus_tag="IWK70_001340"
     CDS             14514..14993
                     /locus_tag="IWK70_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9613902.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            15050..15352
                     /locus_tag="IWK70_001341"
     CDS             15050..15352
                     /locus_tag="IWK70_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9613903.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            15374..16831
                     /locus_tag="IWK70_001342"
     CDS             15374..16831
                     /locus_tag="IWK70_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP9613904.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(17424..18176)
                     /locus_tag="IWK70_001343"
     CDS             complement(17424..18176)
                     /locus_tag="IWK70_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9613905.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(18391..18627)
                     /locus_tag="IWK70_001344"
                     /pseudo
     CDS             complement(18391..18627)
                     /locus_tag="IWK70_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            complement(18637..19595)
                     /locus_tag="IWK70_001345"
                     /pseudo
     CDS             complement(18637..19595)
                     /locus_tag="IWK70_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(19801..21483)
                     /locus_tag="IWK70_001346"
     CDS             complement(19801..21483)
                     /locus_tag="IWK70_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9613906.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            complement(21494..21817)
                     /locus_tag="IWK70_001347"
     CDS             complement(21494..21817)
                     /locus_tag="IWK70_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9613907.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            complement(21840..23246)
                     /gene="lacG"
                     /locus_tag="IWK70_001348"
     CDS             complement(21840..23246)
                     /gene="lacG"
                     /locus_tag="IWK70_001348"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9613908.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            23713..24720
                     /locus_tag="IWK70_001349"
     CDS             23713..24720
                     /locus_tag="IWK70_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9613909.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            complement(25114..>25495)
                     /locus_tag="IWK70_001350"
     CDS             complement(25114..>25495)
                     /locus_tag="IWK70_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP9613910.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
BASE COUNT         7605 a         5311 c         4208 g         8371 t
ORIGIN      
        1 tgcaacagaa ccggtaaatg aatgaaaaag atggaagaat cattattcaa tagctttctt
       61 aaatactccg ttataaaatg tgaacaagta aaaagacatc tatccctatt ttctacactt
      121 ccactttact actatctttg aatcgtacaa ttttcttaac tcttctcgtt tacctcaatt
      181 cattttcaag tggaatcaac tcatcatcta acattttacg ccatgctcgc ttcatttctt
      241 gtgggtattt aaatggtttc gcttctggat gtttggcttt atatccttcg taatccttac
      301 tgttgataat tgctttcata ttaggaattg gatcttcacc tggcatatat tgcagattag
      361 aagcatgtaa ttctttctca gaaacaaacc actcattgga aaaggcatga atcaaattat
      421 cacttgcttg tgtaaaatag cgacgtttaa tgacaaagat atcttcatta ctatctaaag
      481 tccccacatc aaatgcattt aaaatttgtc ggtaaacttc tttgactcct tctgttttat
      541 tcaatttagc caaagctttt tcaatttctt ctcgtgcatc tgaactattc gcagcataag
      601 ttcctagtaa ttgatcaata taagtagaat caatatcata caacttagtg gtattttcac
      661 tataaaattc gatcgttgaa aaatcttctc cctctttttc tggttcttcc tccatcaatg
      721 aacccttcac ggtctcgtac acgccaattt gttcttccaa aagttgaatt tgattgttca
      781 aagttgaaga ttgcaccatg tcatcattgt aatcatcata agtcactaac gattcatacg
      841 cacgattcaa ttcttgaaat gctttgacgt actcgatacg gatttcaagt ggcgtatgtt
      901 catcaatgga ttccatggat gaaacctctt ttaacttacc atatgcttgc ttgaatcttt
      961 ttttggattc ttggtaactt ggataaatca aacccgattc ttctttagct tctgaataca
     1021 gttttgtcgc atcctcgaca ttcttctcca ttgtcgctgg ctttcggaaa gtaacaatta
     1081 gtccttttgt tttttcagga tacgtacggt tcgtacgtga aaaggcttgg atcaatcctg
     1141 catactctag attacgatcc acaaataggg tttgaatcgt aggggcgtca aaacctgtca
     1201 atagacgatc cactacgatt actaaatcga tttgtttccc aaattgtttg aattccgctc
     1261 tttttcgtgc caaacgatta ttgatgtctc cgttataacg ctcaatgtct gctagtgacc
     1321 aggcagtatc ataatactga ttatattcct caataatttt ttgcatttct tcttgttggg
     1381 cacttgaatc tcggctattt tcttctaatg aataggtaat agcgattcgt gggaaatcag
     1441 gatcctccat cgttcgtcct tcacggatag gctgattggg aaactctttt attaaccaat
     1501 cagattcttt cgtcatctct ttgatggccc gataatactt tttggccata tcaatggaac
     1561 tcgtggttaa gatcgcagac ttcgttggac ggccatttcg aaaatcaaat tttgtatagg
     1621 cattgtctgg acggaacatt ttatgaatga ctttttggat atgttcctct gcttcaaaaa
     1681 cagtagggtc caaataactt tcttttttga cagactccat ttgatcgatc atacgattga
     1741 tctgttccgg tgaatactct gcatatgttt ctacttcttt taatttccga tagattttat
     1801 tctctaatga agtcggctcc acggtgtttt catgctctac ttgaaatcct aaaaccgagc
     1861 catcttctaa tgcattcttg atggtatagg tgtgcaagac ctctccatac tgatcatggg
     1921 tcgtacgtgc caattgccct ttggcttgtt tccgattctc agggaaaatc ggtgtacctg
     1981 taaagccaaa ccaagtggat ttcggaaaca tttttttgat ttcttccata ttttcagcac
     2041 ttaacgctcg atgacactca tcaactacaa acacgatatg ttgccccatt aatttttgga
     2101 agcgattggt tccttttttc tcttcctgtt tctttgcata tttcaacgcc gcatctagtt
     2161 tttgacgggt tgtaatgatc acaacattcg cattggcatc tgccaacaga gtttcactta
     2221 actctttggc acttcctgtt cccacgatca agctattcgc tttggcatta ccagaagaaa
     2281 ccccagtatt aaactccgaa gcaaatttcg taaactctgt ggtggtctga ttatctaaat
     2341 ctttgcggtc cactaacatg atcgttcgat cgatccccga ttttcgtgct aaaagcttag
     2401 tagatacaaa actagtcaac gtctttcctg aaccagtcgc atgccacaca taccctgatt
     2461 gatgtttatt tgcggcaata aacaaggctt gaatcgcatg cacttgataa ggatgtaaca
     2521 ccatgagggt tttattgtct tgatcttcac tgacaatcgt ataatccgca atcaagcgat
     2581 gggcatctgg gatatttagc acttgtttgc aaaactcata taagttttct acctttttat
     2641 tatctttcgt tcgccaacta aataaaaact taggatgcaa atccttcggt aaggcatttg
     2701 caaaataccg agtcgtttgt tcattggaaa ccacaaacaa ctgtaaggtc gaaaagatat
     2761 tgttccgaaa catgccttct tcagcatatt ttttgatttg attaaacgcc tgataaaaac
     2821 catcttttgc agttacttgt tttagctcga tttggataat cggtaagcca ttgatcaata
     2881 aggtgacgtc aaaacgtcga tcccgcgctt ctatgttact tccacgtttt gcaatttgat
     2941 ggaccacttc ataagtagag attcctccac caatgtcttg attggaataa aggataagtg
     3001 agacagaacc caattgcgga ttttcccgtt caatcgtgat ccgagccatc ccattttcac
     3061 cttttaacca tttcgcagca tcaaatgggg ttttcgtccg tagcaataat tcggttttga
     3121 tcgtttcaaa ctctttatcg gtcaaaggag aatcattcag ttctgcttga ttatttgaga
     3181 tgattttttg acgtaaattg ctccaaagat cttcttctga ttttagatcc ggacgatacg
     3241 tccactgatt atgtccttcc ccgaggacac gaatcaactg ttcttcgact tccgcttcct
     3301 cacggtgact aattttcatt cttcttccct ccctaaacaa acatcttttg caagaagcct
     3361 tttttcgttt ccttcagcaa atctaactta cgttgatgaa gagcgatcgt atcatctagc
     3421 tgcttgaaga aggcaccaat tttttgttgt tcatttatat ccggtggaaa ctgaaatgtc
     3481 ctttgttcta taccaccttt agtcacatgt accattgtcg ttccgttagt tgattcttta
     3541 attttctctt tatcagtcaa tagccaagta tatagatact gtttgtccaa ttccttagat
     3601 aaaaatttaa gtttccaaat atgataatgg tagattacac gttcttcatt ccacatttca
     3661 ggaccgaagc ttgttgccca taaatataag agatctcctt tattagcata cttgtcttcc
     3721 tctaattcta agtttgaata ataccatcta tcattagtat taaaattacc tactcttaaa
     3781 accctatatt ttccagaatc aagtaattct tgttgtttaa aagctctacc attcaaaaaa
     3841 actaccactt cccccaactt ccgctcttcc caatctcccg taaatcctgg aaaacgaact
     3901 tctggcactt ttgctccgtt tttagggaac attttttgta agaatccttt tttcgtttcc
     3961 ttcagcaaat ctaattcgtg ctggtgaagc atgatggtgt gatcaagttg tttgaaaaat
     4021 gaaccgatct tgatctgttc gtcataagtt ggaagtgaga taggtgtctt tttaccacta
     4081 tcaattgtat aggttccgac ggttccaatg cctgcaacgt cacggatata cttctgaatt
     4141 ttgttattta tgaacgagta gtaaaagaag tttccgtcaa ttttatgctc attcttgaac
     4201 caaatgatgt ttccatcctt aaagtaaatt gggttatcat cagtgattaa taaggggaca
     4261 cctattgatc cgacaccagt tacaagcaag tctccttgag aaactttacc tgaatttttc
     4321 gaatattcat tatatttttc ttctgaaatg taaagataat cggatggttc ctcatttttt
     4381 gcagctgaga caatatcacg agcacgtaaa aatctaatac ctgagttagt ccaatctgat
     4441 tgatgaattc gcttcacaga agtaacatcc atcatatctc ctaacttacg ctcttcccaa
     4501 tcttccgtaa aaccaggaaa ccggatttct ggtcgtgtat cattgctcat gtttgaacac
     4561 ctccagcgca ccttgtaacc acgcttcatt ttcaggtgtt gtttgcaggg aagagatagc
     4621 ttctaaaagt tctttttcta gcacttgctg ttcttgtcgt atcttcttga tgtcatttcc
     4681 gacatgaacc atatcaattg gttcttcttc ttcgaaagta tcgacatagc gtgggatatt
     4741 taagttatag tcattttctt tgatttcatc aaaggttgcc aaatgagcgt atttctcgac
     4801 atcttttctt tcagcgtagg tttcgaggat tttttgaatg ttttcttccg ataatttatt
     4861 ctgatttttc cctttaacaa attcacgact agcatcaatg aataatacat ctcgagtttg
     4921 acgattcttc ttcaatacaa tcaccgtcgt tggaatcgat gtaccaaaga acaagttcgc
     4981 aggcataccg atcacagcat aaatactgcc gtcttctaat aatttttgac gaatgacccc
     5041 ttctgcagca ccacggaata aaaccccgtg tggcaagacg atagccatag tccctgtttc
     5101 cttcaaatga tagaaaccat gaagaagaaa cgcaaaatct gctttggatt ttggcgctaa
     5161 tttcccgtag cgattaaacc gagaatcatc taagaaggtc gtatctgctg accaattagc
     5221 cgaataaggt ggattcatca ctactgcatc aaatgtgtaa ggttcatctg ttggccagtc
     5281 cttattcaac gtatccccat tgcggagatt catttcttcg gcatctaccc catgtaaaat
     5341 caagttcatt tttgctagat tgtatgtcgt tgtatttagt tcttgcccat gatatttaac
     5401 gttatctgga tgagttaagt agttacgtac atttaacatc aaggaacctg aacccatggt
     5461 tgggtcaaag acactaaaga atcgtcgttc tttttgatcc aacgtcacga tttgtgccat
     5521 catatcagaa accatatgag gcgtgtaaaa ttctccggct ttcttccctg cttctgaagc
     5581 aaattgactg atcagaaatt catacgcatc cccgatcaca tccccatcgt gacctaaaac
     5641 ttccacgtca ttcaatttct tgataacttc ggtaatcgtc acatttcgtt gttgctcatc
     5701 tgttcctagt ttttttgatt ggagatccac atcatcaaat aacccattaa attgattgta
     5761 ggtagatgcc aattgcacaa aggctttgtt tagatcgttc aattggaacg ttgcttgttt
     5821 tgcttgatcc gctaagacgt tgaaaagata ctctggtgag atcgaatacc ccaaaatatc
     5881 aacgattgtg gcaatcaggt cttcttttga ttcttcatct gaaagtaatt cacgatacaa
     5941 cattgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
     6001 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agtcccaata aataattttt
     6061 atattctgac gcatccattt tactacggag attatctgcc gcactaaata atttttgatt
     6121 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
     6181 tattttagca tactcccttt tagaaaaagc aaaaaaaaaa gaaaaaatct tattcttgat
     6241 aacaagctac gtgacgtata tgcctaacaa aaatagaact tttgaatcaa tcttaaatga
     6301 aggtttaacc acagaaccgg catccattat ttttgatgaa gttatcgatg acgttgaaga
     6361 tagtaagtat cataaaaatt taaaagaact tttttatttt gcactcaaca atggaccaag
     6421 tttgttcact ggtatatcag tacctgatag tgataatcca ttaattatgg caaaaattta
     6481 cttaacaaaa tggtgtaata aatacatcag ggataggaat aacccagcat taaaaaaacc
     6541 tcttaaaaca tttggcgaaa aagatgctgc tttaactact agagtagcag caaatgctaa
     6601 tattgatgat caaaaagtat tgaatgacta cttgcgtgga cattttttat atatgagcgc
     6661 tgagaacatg aacggaagta ttttagagga atatttagcc gaagtgttag aacctgaagg
     6721 ttggatttgg tgcgccggct ctgtatatcg agctgttgat ttctgttatt taggaacctc
     6781 accaattctt ttacaagtga aaaataaata caatacagaa agcagttcga gttcagctat
     6841 aagagtagga acaacaatta gaaaatggaa tcgtttaaat aagtcgacaa aaatttctgg
     6901 tttggatagc cctatcccca attggaaagc attaatagaa atgactgaag ctagtaagga
     6961 actggcagct aagttaactg aaaatagtta tttagcttat attaatgaaa aatccacacg
     7021 agaattatgg acactcgatg actaacaata tccttattag tatctatcca cgatacatgg
     7081 aaaaaattat aaacaaacaa aaaaactttg aatttagaaa tttctatctt acctcagatc
     7141 cttataatat cttttggatt tacgaaacaa tccccaccaa agctttaaag tataaattga
     7201 ttgtaaaaaa tccaattact aaacttaaga aaaatcaaca ctacttttta ggagacgatg
     7261 agtttgaaca attagtttaa aagggaaaat atgcttctat cattacaagc tcgtttggtg
     7321 taagatgggt ataagtcatt tatgttcact ctccttgtat gctttagcgg gtattacaat
     7381 ttgagtgtaa cataaatggc ttttttattt gtctcgctta attatacaaa cggcgaatta
     7441 taaataactc ataaaaaata ttttgacttt caaaatcact tttgctacca gaagttgagg
     7501 cgaagtgttt taagcatagt tgtaccctag tttgaccgtt aaaaataaat aacgatcgct
     7561 agagtgcaac tatgtttgtt tgccctaccg tcagcgtcgg cttggcctct cacatccatt
     7621 accgtcggga ttatctccgt tacataccct tacaaaatac gccctgtaag cattgcatga
     7681 aaccaccgtg catttgcacc gtatttccgc cctagccgtt caaatcggtc gctaccacca
     7741 ttcagaaaca cgtgcggtaa tggaaggttt ggaggaagct atccagacca ctaacgattg
     7801 tccctatcgt cgtacgtttc actcagatca aggttggtcg tatcaaatga aagcttatag
     7861 aaacaaattg aaggagtaca aaatctttca aagcatgtct cggaaaggga actgcttaga
     7921 taattcactt atggagaatt tttttggctt attaaaacaa gagatttttc acgggaaagt
     7981 ttataactgt ttcgaggaat taaaatcagc catcgatagc tatatctatt actacaataa
     8041 tgaacggata aaacagaaat taaactggca aagtcccgtc caatttcgta aaacaaccgt
     8101 tacggttgtg taaaaatatc attttagagc atgaaaaaaa gagtggattt ctccactcca
     8161 aaaaagtcta acttttgggg gtcactacat ttttgatcct ttttatttga ttaattatat
     8221 ccagctggat aataattttg ggggcaacat ttctatctta ctattatctt cctaaagtag
     8281 tagactttct tattgggaaa aattacaaca aaaaggagtg gcttagttga caactccttt
     8341 ttgtttacaa atcaattgga tatctataaa ttgtattttc taacgaattt tatagacatc
     8401 tgatttgtca aattaagcta gacaatatgt ctttatctat gcagctcaaa aatacttcca
     8461 atggtgttcg ataatttaat gattttcttg gaatatggtt tcttttcgag gcaacggatt
     8521 gaataaaggt ttcatccgtg tccctaaagt ccattgtttt aggtaagcca tcctgcttga
     8581 gcccatgcac tcggtactga tgagaaaaaa ttgatgcctg tttctcctga ggaatgccaa
     8641 ttccctgatc ttttacttca ataatcacgc tacccttttg gtttgaaatg gtgacaatta
     8701 ccagttttcc ttcaccatat ttgattgcat tgtcaactaa attcgaaacc gcccgataaa
     8761 tccaacgatc tcgtccatga atcagggttg gttgatcttc agtaaacgca aacaaaagat
     8821 tcggataatt cattttgtag gcatcacaaa cttccgcaca gatcagtgaa acgtctacga
     8881 cttccgtgct aagatcctct gagttattgg ataatgtcaa aatatcgtca atactgtcgg
     8941 tcatccgatc taaaagcatt aaatttcctt gatcggcatg ttgccgttct atttccagat
     9001 tcgtacgcaa aatagcgatg gcatttttct gctcgtgggt cagataacta tttaatcgtc
     9061 gataatcctc caaatgacca tcgaacttat cctttagctt attaaaagcc gcatttaatg
     9121 cctcgtcctt cacagtattt ttaggaactt gatcaataaa cgttaaatca cgcgtaatcg
     9181 ctaagatttg attactgtaa attttgcgta aaaccaacca aagaataaac gttaataaca
     9241 tgacggcaga acaaataacc agcaccatcg ctggtaaata tttaaatagt tcgtcctggt
     9301 aagctttatc tatcatcgaa atagtcataa attgattgtt ggaagatttc gaagcttcca
     9361 tctgcgtctc tgcttgaacc gtcttaccct cttccataaa ataaacatct tcacctaagg
     9421 tagatgcgat actgattccc tccaaattat ttctcagaaa gaagaaactc actaaaatca
     9481 cgcctgtcac caacagtgaa aaagccagca tcgttaacgt cattttaaaa tttaatagca
     9541 tagctggtac ccctttgctc gaatggtttt taagacctct ttttcagctg cttcagccag
     9601 tttctttttt agacgagcca gatgcactcg cagaaccgac gaaaaaggat caaaatcttc
     9661 atcgtagaca tgttcagaaa tttcctcaga cgaaacaacc gctggatgac gctgagcgat
     9721 gcacagtaag atatcaaatt ccttgggttt caggaccacc tctgtctttt gatagctgac
     9781 ccttcgacct aatggatcaa tcttcaaagc accaattgaa atctgcggca gcgcacgtcc
     9841 atgataacga cgaataaccg cgcgtattcg tgcttttaat tctagtaact caaagggttt
     9901 gactaaataa tcatctgctc caaaatccaa gccttttgct cgttcttcaa tctcatcacg
     9961 tgcagtaata atgataactg gactttcgac ttcttcagca cggagacttt gtaaaacaga
    10021 taatccatct tttttaggca gatttaaatc tagtagtatc ccatcgtatg aattgatgaa
    10081 ggccttctct tctcctgact cgccatcata agctacatct acagaaatat tctcctgctc
    10141 taggcccttt tttacggata aaaccagctc acgattgtcc tcaaccacca gtacacgcat
    10201 gtatattcac tcctcatacg gatttagtaa ttacacttat gctttttagc atagtacacg
    10261 ctcttcttca aagtccacgt tctttttgtg aagatttgaa gaaattcata cattctgtta
    10321 aaaaacacat aattaagaat gattttactg tttgaaaata cctagaatag aaaaagactg
    10381 gaacctttcc tccagtctca ctactttcta tttgcttggt tttcttgtat atacttttca
    10441 aagttcgttg gtttatgacc aataagcctc gttagaatac tgaaaattgc catcaagtca
    10501 ttaagtacca catgtaagaa aatctctcta aagaaaaacc ccttcgtttt aactcactta
    10561 ctattttaga attcgttccc ctaataatgc agcatgattt tacgctttat gtataaaaaa
    10621 agactgcaag caattttgct tcacagtctg atttcttatg atagtgagct tgttaacact
    10681 tccaccggtg tagcagtttc tttcaatacg gcatttaatt cttcaatatt tttacgccct
    10741 tgcgtattca tccactccat cgctgcttct tttccatcaa cgatatagct ttctacacca
    10801 tctgcccaag tcgcacggcc gcaaagtaca ccattgaagg ttgagccggc atttttcgcg
    10861 aagcgcagtg tttcttggaa caactctgct tttacaccgg cgcttaggaa aataaatggc
    10921 aggttagtgg cttcagcctg ttccttgaag aagttagccg cttgtgcctt tgtgtacacg
    10981 acttcgtctg tcccatagcc ttccacataa ttcatattga cgggtacttc cacctttaac
    11041 acatctacgc cataacgtgg atcagaaaat tccttcatca tatcgttgac tttatgaggt
    11101 ttcttaatgg cgtattcttt cgaagtcgca tcagagatgt tcgcgtcata agaaacaagc
    11161 tccaagaaga agggtaagtt ttcagcctga cattctgagc cgattcgttc aataaaggca
    11221 tgtttttgat tattgatttc ttctgcttca tcgacatcat agtaaagtag gaacttacaa
    11281 gcatcggcgc ctgcttcttt taaacgttta actgaccaga tcgataataa atctggtaaa
    11341 cggccgggag ttgacgcatc gtaaccagtt ttttcataag ctaacagcaa gccggcattt
    11401 tttgctctaa cgtcagctgc tggcaagcca tattcgggat ctaataaaat cgatgaggca
    11461 tatttcgtta attgtgaaga aacaatttct ttaaattcaa cgatttctga ttcttctgct
    11521 tcgctgccct tgaattttgt gatcatcttc ttcaaagccc cgcgttgatc aattgctaag
    11581 gccccaatca ctccatcttc attgcatagt tgcttgatat attgacgttt ttcttcgttc
    11641 atttccgtat cctcctacac tttctccact aagatttgat catatagttt ttgataattg
    11701 gctaattgga catagccagt cgtcgcttcc tgagcattca gcattcctaa aacattaccc
    11761 tgttttaaca cggcttcatc cgaaagacct tgttgaatcg cagcggtcaa tccggcaacg
    11821 gtcgcatcac cagagccaac tggattgacg acatcaatct ttggaattgt cacgcaataa
    11881 aaagtctcgt gatgtttggc aaaggctccg tcgcccccca ttgaaaccac tatccattca
    11941 atttcatcaa acatcgaatc tgacaaggcg gcttttaaat ctgataattc tgttgagatt
    12001 tctcgtccta gcagaccagc cagctcttcg gtattgggtt ttattagctt aggctttaca
    12061 ctgcctcgca acacttccct taatgctgta cctgaacaat ctaaaacgac aggctttggc
    12121 tgacaaactt gcagcatttg tcgataaaaa tcagcaggca atccttctgg caagcttcct
    12181 gaaaaagaaa tgatttcggc tttaacagct aatttttcga aatgttgcaa gaacgccctt
    12241 gcttcttgtt cagtaataac aggtccgttt tctaggattt ccgtctgttt tccgtcatgt
    12301 aaaacagcga tgcaattacg tgtctctccg ctgataggta aaaagtcatg agcaatctca
    12361 ttttgagtca ggtccttttt aatctcttca cctaaaaaac cgccgattaa accactagca
    12421 atcacctccg tatctaattg cttcaagata cgtgtcacat ttaggccctt accgccagct
    12481 gttttgcgaa catttcttac tcgattgact gtgtcgagct tgaaggtttc taatggataa
    12541 gaaatatcaa tcgatggatt catcgttatt gttaaaatca tttgtccacc cgcttaatcg
    12601 tggtattcgc cacgatccca tttctcgata aattcatcga agaaatgcgg atcagactgc
    12661 ttgtcactgg ctttttcgac gtgatcgatt ttggcgatca attttttgtt ttcttcagtt
    12721 tcttgataat cggcattgat gaaggcctct aatatgtcac agatcaaaaa ttctcccgta
    12781 atccgagcgc caaagccgat cacattggca tttagttgtt ccttcgcata gatagcagtc
    12841 gtcatatcgc gaaccagcgc actgcggata ccaggtactt tattgacagc gttgttaatc
    12901 ccaacacctg ttccacacaa acaaactcct agatctgctt ctccgctagt cacggcctct
    12961 ccgacttttt ttccgtagat cggataatgg gttctggtaa agtcataggt ccccatatcc
    13021 aacacttcat gtccttttga ttttaagaaa tctgatactg ccatttttgt atctgtgacg
    13081 atgtggtcac agccaattgc gattttcatt tcattcatcc ttcctaatta cgccatttta
    13141 tttaacatgt cgacacgtac ttgatgacga ccaccatcat aatttgcttg tagaaattcc
    13201 ttaacaatat tcaacgccaa ctcatcgcca acaatttttg atccaagtgt gatcatccga
    13261 gaattattat gtttacgggt catatacgca ctgcgctcat ccgaaacttc tgctgctacc
    13321 attcctttaa cctttgttgc cgtgataaag ctgccagcgc cgtagccatc aaccgcgatt
    13381 cctaaactac cttcgttttc attcagatca tttgcgatcg ccaaagttga ttccacaaaa
    13441 tcttctgaag ccgtttcact cttatctact actgtaatac cttctttgat caattttttc
    13501 ttgattaatt cctttaattc catgccgtca ctgtctgaac caattactac acgcattttt
    13561 gtttcctcct attttttact gaatgatttt agtgaattgc tgataataac tcttcagctc
    13621 ggctgaaaga gtagaatccg taaataattt gtcgatcttg cgcaaatcat agaaattata
    13681 aaaatcgcta tggtcgaatt tgtttgcatc tgaaaccaaa catttttctg ccgcattatc
    13741 taaaacaatt ttttgcaagg taccttcttc aatactgaaa ttcgaaacct ccgaaccctt
    13801 caatccattc actccgacaa aagccttctt aataccgatt ttttgaacag cttcattggc
    13861 aattgaaccg acaaaggctc cagtcttttc ccgatatagc ccaccgatca aatacagttc
    13921 aaatcttttt atttcactta atagattgaa aaccggcaga ctattggtaa cgattcggag
    13981 agatgacggc ttgatgtatc gtgtcattaa ttcgatcgtt gtacccgatc caagaaaaat
    14041 cgtgtcgtct tcttgattga tatgagctgc cgcattttta gcgatgtatt ccttctcttt
    14101 tgtatgcagc ttcttttttt cagtatgaga aagctcattt gcttctatag aagtcgacat
    14161 ttccagataa tcgatttttt gtgcaccgcc atgaacgcgt aaaagctttt tcttctgcgc
    14221 gagttcctct agatctcggc ggatcgtcat atctgaaaca ttcagtactt cctgaataaa
    14281 tttgactgtt atcgtcccat agcgatcaca catcgctaag atcgaacgct gtcgttctgt
    14341 tttcagcata ttctgtacct ccacgacctt ataataaaac atttacgaac atttttcaac
    14401 acatattgtt cgtttatttt cgtttatgcg ttgacaatga ttttgtaagc ggttatactc
    14461 aacttgtaaa catataaata aacataaacg aactaataaa ggagctgttt tgtgtgaatg
    14521 aattcttttt caaagacact gtctatgttt ctgacaagca gtcgcaggaa gaagtgttcg
    14581 aggaagtcta tcaagatctg ttaaagaaag aattggtaac cgaagatttt ttggagaacc
    14641 ttttagaacg ggagcgtaat tatccgacgg gattagatct ctcacctgtc tctaaaatat
    14701 tgccagatat cgctatccct cataccgaaa gtgagtttgt gcgaaccacc cgtatcattc
    14761 caatcaaagt aaaaaatgaa atccctttct acaacatgat ctcgccaagc gatcagattt
    14821 ccgcttcatt tcttttcatg atcttgaacg aaaatggtga agcacaggct ggcatgttgg
    14881 ctaatatcat ggatttcatt aattctgttg acgaagaaaa actgttttcg tttttcaaat
    14941 tagaggacac agaagcgatt tatcattttt tagaagaaca tttttatata tagaagtctc
    15001 tgcctaaccg caggataaaa aataaactta ttttaagggg agaattatta tgatcaaaat
    15061 tttagcagca tgtggagcag gcgttaattc aagccatcaa atcaaaagcg ctttggaaac
    15121 tgaattgaaa aatcgtggct atcaagtcac ctgtgacgcc gttatgatca aagatatcaa
    15181 tgaagaaatg ctgtctcatt atgacatttt cgcacaaatt gccaacaccg acttgggttt
    15241 tgacattaac atccctgtcg ttgatgcagg tgctattctt tatcgtattc ctgctatggc
    15301 tgagcctgtt taccaaagta tggaaagcgc aatccaatca ttaaacaaat aggactaaag
    15361 aaaggaaaag catatgaata caattattga tattgccaat aaagtattta cccccctaat
    15421 taatttaggt gcggcaccgt taatgaccat cgtattaact gttattgctt tattttttaa
    15481 agtaaaaccg tcacgcgctc ttgaaggcgg gctcaaatta gggatcgccc tgactgggat
    15541 cggagctatt attggaattt taactactgc tttttcagac gctatgacag cttttgttga
    15601 acgcacaggt atttcattaa acattacaga tgtcggttgg gccccactag caaccatcac
    15661 atgggggtcg ccatacacgc tttatttttt actaattatg gtgatcgtca atattgtcat
    15721 gttagcaatg aaaaaaacca acacattaga tgtggatatt ttcgatattt ggcatttagc
    15781 aattgttggt ttgttcgcga tcttctgcgg tgctaatctg tttattgctt ctgcattagt
    15841 tattttcatt ggtgtattaa aaatttggaa ctccgactta atgaaaccaa ccttcaatga
    15901 cttgttaaat gcgccagaca gcaacccgat gaccacgact catatgaact acatgatgaa
    15961 tccgattatt atggttttcg ataaaatttt cgacaaaatc ttcccttggt tggataaata
    16021 tgatttcgat gctgccaaat tgaatagtaa aattggcttc tggggatcaa aattcgcgat
    16081 tggaatttat ttggggattt ttgttggatt actagcagga caaacaccaa cgcaaatttt
    16141 ctcgctagcc tttaccgctg ccgtctgttt ggaattgttc tctttgatag gcgcttggtt
    16201 catcgcagcc gtagaaccgt tatcacaagg gattaccgat tttgccaata aacgactaaa
    16261 aggacgtacc atcaacatag gtttagactg gcctttcata gcaggccgcg ctgaaatctg
    16321 ggctgccgct aacgtattag caccaatcat gttgctagaa gccatcatct taccaggaaa
    16381 taaattattg ccactaggcg gaatcatcgc aatgggtgtt accccagcac tattggtcgt
    16441 aactcgtgga aaattaatcc gtatgatcgt aatcggcgcg atcgaattac cattattctt
    16501 atggtccggc accttgatcg ccccgttcgt tactcaaaca gctaaagcag tcggagcctt
    16561 tccagccggc ttgaacgggt ccgccatgat ctctcataca acgatggaag gcccgattga
    16621 aaaattccta ggttatttag tcggtaatgc ttctcaagga caaatcgaat ttgtgatata
    16681 cgctgcctta gcattaggtg catacctttt aatcttcatt tggtacgctc gtcaaatgaa
    16741 aaaacgcaac gctgcatacg cagctgaaaa agaagagaat gcatctccgg caacggcgca
    16801 taatatgact tatgcagccg aagctaaata agctaaaaac accccgaatg tctttagact
    16861 tcggggtatt attttaccta taaaaaataa atagatgtaa aatccatttc aaaaagcata
    16921 gcccatctct tgccacaccc aataaagaaa ttatcaacgt ttatgctttt tttaactcct
    16981 ttaagcttgc ctttgttggt attcctcaac gattcttttt gtgttcttgt ttgttttgtg
    17041 ctttttgact ttttgttgtt taattgcgaa ttccttgatt tcttttttcc tgaatcgctt
    17101 cgcatcccgc ggttatgcgt gcgatttcat cattgctttt tctgatctaa ctcgtttcat
    17161 gtcaaagagc ttcagctgac ttagtgtcca tggttgtttt gatatccctt ttcctcctta
    17221 ataaagcggt gattgatact gctatttttt ccttcacata aacataacat cccaaaaaaa
    17281 tcgactatct tttttcaaat ggtgtattca agtattcgga ggaattagta atgatgcacc
    17341 ctaaaaataa cataattcgc ttaaattcaa ggctaccaaa aaactaccaa gttctaatta
    17401 tctagagttt ggcagtttct catttacaca acatattttt cttctttttc gattttttca
    17461 tagtgctttt gttcatcatc atccgttata actactgtta catcattcag attataatat
    17521 actgaaaaat ctcttttccc aattttcgaa tgatcaatca ataaaaagcg ttcaacagaa
    17581 ttattcagag cgatctgttg agtttggccc tcttcaaaag tcgaagtcat aattttctgt
    17641 tcatttaaag cattacagct gaaaaatgct ttatgaaaat tcatatcctg tagcgattta
    17701 ttcgtgattt caccaacaaa gcattgagtc gtttcccgca tctcgccacc taatagatag
    17761 atttttcgat tcccttttcg cttatttaat tcttcaaaaa caggcaaaca gtttgtaaca
    17821 atccgcagtg aatccccttg aatgattttt gacatgagct cgattgtagt tcctggtcca
    17881 agaaatatcg tatcaccatc attgatcatt tcattcgcct tttccacaat ccgctgcttt
    17941 tcttcttgca gcataatctg tttttctcta tgagaaagct ccacatgccg ataaaaatcc
    18001 ttcaatttcg ctccgccatg gacgcgttct aataatcctt gtttctccat ttcgtccaaa
    18061 tcacgtcgga ctgtcatatc cgaaaccgaa aacttctcca taatatcgct gactcgtact
    18121 gtaccgttaa cctctaataa cgacatgatc gcttggaatc gatccgtttt taacatttca
    18181 tcgcctcttt tctttttaag actatcaaat tcttaagaaa agtgctagaa caagttttct
    18241 taagaagcct tctgcaggaa ggaaagcaaa atttttcaga atttgcaagc tgctattcta
    18301 aaaaataaaa ccgaacgagc atgactgtca tagtgacagc cttgttcatt cgacttttat
    18361 ctttaataaa tcattttatt taatctgaag ttattgtgca agttcaaaaa caaagtagtt
    18421 aatgtctcct cgatatttcg aaatcgaata ctcaacgggc agacgttcta gcgaaaaacc
    18481 ctgtgtatgg aaatagaaca ttggatctcc ttcttccaca tctaacaaat cgctgatcgt
    18541 atcatccgct ttgattactt ctagttttct ggtgaccgtt ttaattggat aacccatttc
    18601 ttcaaaaact tgatacattt tttcttttct gatttgtcaa attaagctag acaatatgtc
    18661 tttatctatg cagctcaaaa atacttccaa tggtgttcga taatttaatg attttcttgg
    18721 aatatggttt cttttcgagg caacggattg aataaaggtt tcatccatgt ccctaaagtc
    18781 cattgtttta ggtaagccat ctcgtctaag caagccgttt gagtgctcat ttagccctct
    18841 ttgcgaaggt gttcctggat ctgcgaaaaa gatggcaata tcgttcgagt tacttataga
    18901 tcgccagttg gaaaattctt ttccacaatc aaatgtcatg gatttaaaca agtgtttagg
    18961 catggatttg aaccaatcat ttaacctgtt ttcgatatct tcggctcgtc tgcctgtcgg
    19021 tttcaaagta ataataacct tggataaaca ctcaaccaat gtaataacag cgctcttgtg
    19081 tttaactccc acaatggtgt cgccttcgag atgaccaaat tctttagtca ggtgtggata
    19141 gagactgttc cgttcatgaa tcgttctgcg aaaggcctgt tttccacgct tttcttgatg
    19201 attattcggt ttacgtttcc ccttcatagg taacgactgt gtgtcaaaaa ggcctttttt
    19261 aaacatccga tagagtgtac gaacagaaca atcaattgga aactcgccac ggccgataat
    19321 cacatcgggt gaccaccctt gagctacctt ttcttgaaca tatgttgatt gttcatcaga
    19381 taaaacggtt gaacgtcgtc cacaacgaga tttattttgt ttgtattgac ggtaataatc
    19441 tagcactttt ttccttgttt gagaaacgaa taaacattat aaatggtttg tctagagcga
    19501 tttaaacttt gtgcgacaag agcgacaggt ttctcgatat tgaaataaga ctctatgatt
    19561 actagttcgt ctgttgtaag atgggtgtag gtcatttgta ctcactcctt attaattctt
    19621 tggtcggaac tattttgagt gtagcacaaa tgatcttttt agttgtctag cttaatttta
    19681 caatcgacgc ctcaaaaaga atacccgaaa tttaaacagc aaaaaaagat agcgcttacc
    19741 attgtgttta agataaaacg ctagctaagt tggctagcgc tttattaatc taagcaagct
    19801 ctaaaattgc ttcaatacaa aatcaactgc tcctttagga tcacgtgtta aattgatata
    19861 ttctgcacct ttagttgcaa ttacttttat ccctaatttg tctgcatctt tacggagatc
    19921 atccaaatag gaattcactt gaggtgctag aacgattaaa tcaaaatttt tcatcatatc
    19981 ataatgagaa ccataagctc ctgctttagc agaaagatta acacctgtct cttttgcgcc
    20041 ctcttctaat gcattagcca acattgcact cgttccggca ccagcacaaa gtactaaaac
    20101 gcgctttcct tcaccaaccg atgcttttgc ttcatcaact ataactgccg cagttgcacc
    20161 aacttcttgg atttcttcga ccaattcttc ttttacttct tctgcagcaa tgttttttaa
    20221 ctcttgttct actaattcag aatcataggc cttaacaaaa ggtaaataaa taatcgcatc
    20281 gacaacaatc aatactaccg ctaatacaat cgccaacaca ctaaacccgg ttcctaaaac
    20341 caagccaatc ggtgcgggcg tcgcccaagg caatacggat ataaagctat tcatccccaa
    20401 gacatcgaca aaaagtttga atatccatac gttagcgatt ggtgctaata aaaatggaat
    20461 tagaaagtac ggattcaaaa taataggagt agcaaatagt aacggttcat ttaccgcaaa
    20521 cgaaactgga ataaatgaag ctttaccaac tgctttcaat tgttttgatt tagcgaacaa
    20581 catgaacaga aaaggaacaa ctagtgtcgc tccagtacca cccatcgttc caacaaagtt
    20641 tcctagacca actgttaata cttttgaggc ttgtgcaccg tttttaaata attcaaagtt
    20701 agtttctacg ttcgcataaa taattgcagc aatcgctggt tcaacaatcg atggtccatg
    20761 aacaccaaca aaccagaaca ttgccatggc tccccaaatg attgcaatac cgacatatcc
    20821 atcagcagca ctaaataatg gctgcaataa ttgaattaca cctgctgcaa atgatgttcc
    20881 aaaaaattga cgaactcccg tatcaatcaa aacggcaata aatactgcaa ttgaaaaggg
    20941 gataatatct ctaaacatct gtgtgattgt tccgggaact tcttttggca tacggatcgt
    21001 caaatctctt ttaacacaga atttatacac atttacagta acaaacgcag aaacgaaagc
    21061 tgctaataat ccctttgtcc ctaaataatc agtagctatc ccaccatcaa tttgatcagc
    21121 tgccatcatt acaaaaccac aaattgacgc taacataaca gaaacttcat taatctgctt
    21181 acctgaatca gataattgac ggttcattga attagctaaa catcgagctg ttgtagcaac
    21241 aaccaaaaga ccaactaatc ccattgaata accataaact ttccaaagcc acgtcgagaa
    21301 tgcctctggt aacgtcacgt taaatacttc tggaacagcc gcaatcataa taaaaatact
    21361 tgaaaataga atagctggca tagcagttaa aaagccatcg cgaattgccc ctaaataaat
    21421 attacgtgag attttttcaa aaaacggttt tcccttctca atttgcttaa taatcgtatt
    21481 catttttctt ctcctatgct ttagcaaatt tttctttgta caattcaaca aaatcattga
    21541 taagttctct taatagaatc gttgtcatta aatggtcttg cccatgaata aaaattacgc
    21601 ctacttccat ttcgttccct gacgcttctt cagctaaaca tttcgtttgt gagttatgcg
    21661 ctaaggttaa attttcatca gcatccgcta ataatcgttc aacattatca aaattacctt
    21721 ctctcacttc cttcaacact gttaataatg aacttctggc atctcctgca taagcaacaa
    21781 tttcaaatcc taataattgc aattctttcc tatccatttc cttgccctcc taactatttt
    21841 tatattatcg tttttgtttc gctaacaaat ttataccaat atgcagactc tttcggatat
    21901 cttttttgtg tatcgaaatc tacatagaat agtccataac gcttagtgta tccattaacc
    21961 caagagaaaa catccattaa tgaccaaatg aaatacccct ttacattaac tcccattttg
    22021 atagcatcgg aaatcgcatt aaaatgctgg cgtaaataat cgattcgatc atcatctaga
    22081 ataatcccat cttcaaaatt atccttatac cctagcccgt tttcagtaat gtatatttta
    22141 ttataatttg gatattcgtc ttggattcgt acaagtaaat catataaccc ttttggataa
    22201 atgatccaat cccaatcggt tttcggaaga tcctcacgca cgattctctc gccaatccct
    22261 ttaatgcaat agacttggct acctttttct ccggtagtgt tgtagtgaag tgtgctttct
    22321 cctttgtact ctttaaccca atggctacca tagttattga taccaagaaa atcatttcga
    22381 tttgctgctt ttttcatctc tctaaaatca ttagaatcaa tatctgcaga ttgcccattt
    22441 gctgctaaaa tctcattaat atgtgccatt gtttcatctg agtaataccc taaataagta
    22501 gcatctaata agaacttaat tgataacgca tcatctaatt tcgcagcatg cttatcttct
    22561 tctttgtcac ttgctggata ttttgtctct agcgagtgaa caacaccaat ttccccttta
    22621 tatccaccat ctttaaaaat gtttactgtc tttgcgtgcg catacatcat gttgtgtaaa
    22681 caagttacag tcttctctat atcaaattga acttctggag gaaacgatcc gatcaaatat
    22741 tgatttgaag caactggata aatctcatta aatgtactcc aataatgaac ctcagaaaat
    22801 tcatcgaaac agaactgtgc atagtttacg aaatgttcaa cattttctcg atttaaaaaa
    22861 tctccattat caaatagttc tttaggggta tcaaaatgat gaagagttac aaaaggaata
    22921 ataccatgct tattcaattc agcaaatacc ttatgataat attccactcc tgtttggttc
    22981 acttccccat agccttttgg aaaaatacgg ctccatgcaa tagacatcct aatactatca
    23041 attttaaatt ttttgcataa ttcagcatca actggatatt gattataaaa atcactagct
    23101 ggatcagcag tatagcgtcc cttttctaca aggaaagtat cccacgctac ttgccctttt
    23161 ccaccttctt ttgtagagcc ttctgcttga taagctgccg tagctcctcc cataataaag
    23221 tcttctggta aagttttata attcatttta atctctcctt gaaaattagt ttgatcaata
    23281 tgccaataat ccataaatca cctcagcata attttaacat gtataagtaa atttttttac
    23341 acaacgaaaa accgacctcg aatatctatt ttattgcgat atacaagcac ttatatccct
    23401 tgatgtaaaa taaatatacc attcaaaaac cgttttgtaa acgttttttt gtaaaataaa
    23461 tttatctttt tactaattta atttacacga aacaagaaaa cggactgatt cccactaaaa
    23521 tcaatcccac ctactaatca ttctccaagt ggctatccat tatcgattca atcttctctg
    23581 ttaatggatt aggctctctg ctgatatcag agtttcgttt cctgctagaa aaagtaattt
    23641 aagttactaa aagagcagtt tcgatttact ttaacaaaga aaaggattat tatatcccta
    23701 ggaggatgaa caatggttac tattagagat attgcaaaaa aatctggtta ctcaacggct
    23761 actatatcaa gactttttaa aggtgatgag tcactctcta ttacatctga tactaaaaac
    23821 aaaataatcg ttacagccaa atctttagga tatgatctga caaaaattaa gactactctg
    23881 tacaagattg ccattctttt ttggatctca gaggaacagt taaaaaagga tgtctactat
    23941 caacatgtca aagatgattt gaaaaattat agtaaaaaag ctaatctaga tttgattttc
    24001 atctctaaaa acgatggtgt aaaaagcatt cctgacgata ttactggatt tatcgcaata
    24061 ggacaattga caaaaaagga attgtactac ttaaatcaaa aagggtataa gggggtaatc
    24121 cgaggaataa acccattacc tgaacacttc gatactgtag aacctgattt ccacgaaatg
    24181 gccagacaag caattaacta ctttattaat gaaggtttca caaaaattgg atttattgga
    24241 ggaaatttct ttaacctaga aacgggtaaa gaagagattg atagccgtga atatgccttc
    24301 cgatatcatt tagaaaagag cggactactc aatgaaaagt atattttctc ccaaggatca
    24361 tttactgttg acgaagggta tcaattaact cttaaaatga aagctgagtt aaatgaagat
    24421 atgcccgagg catgtttcat accatctgac actatagctg ccggcgttct tcagggctgc
    24481 catgaattgg gtattaacat ccctaaagat atggcagtaa taagtattaa tgatgacgac
    24541 atcgcaaaat tcgttgcacc accacttaca acttttaaga tagatatcga agagatagct
    24601 aaaacctgtg tagatttatt aagtgatcaa ctggtatatt cgcgggacat atcaaagaaa
    24661 acattaatcg gcgcaaaatt gaccgttagg aaaagtttta tccctaaaga agaggtgtaa
    24721 aataagtaat tgaaacttat ctttaaaact ataattgtgt ccacatagta gccgagcaat
    24781 cgggtttatg cctattatgt tttccataaa taatttgatg gtgatatttt gacgtaaata
    24841 caatatgatt ttttcatcgc caaattgtgc gcgctaattt ttgtttatca ttcagaacat
    24901 ttcctccttt ttatctaagt atgaggttgg ataaacgctt catcttagta tcttggggga
    24961 ttttttatct cgttgggaat atatcaagca tcagagcgcg caaaaagaac tggtcgaaaa
    25021 atcaatctac cagttcaatc tcattcactg tgatactatg ttttatacag ataacgccat
    25081 attataaaac aagttgtatt ccgcaaatgg gttctattca actcttaaac tatctataaa
    25141 agattcagcc aactccatta gcctctcgta atttagttgg tgaacagcac ttttttcaaa
    25201 aaattttgaa ccaattccta cataagaggc tccattgttc aaatagtttt gtgcattagt
    25261 ctgatctacc cctcctacag ccatcaaagg taaacttcct aaaggggctt gcacttgatt
    25321 gaaatattcc ggtccaagac agttggcggg gaacacctta acaatatcag cacccatctc
    25381 aaacattttg aatatctctg tgggcgacat ggccccagga attgttagaa ggttctgttg
    25441 cgaagtttga aaatcaaacg cgccctctct gaactccaaa tatcttaggc tgtta
//
LOCUS       DACDTO010000032        25119 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDTO010000032 DACDTO010000000
VERSION     DACDTO010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.032"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            12..84
                     /locus_tag="IWK70_001351"
     tRNA            12..84
                     /locus_tag="IWK70_001351"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="IWK70_001352"
     tRNA            88..160
                     /locus_tag="IWK70_001352"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="IWK70_001353"
     tRNA            168..249
                     /locus_tag="IWK70_001353"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="IWK70_001354"
     tRNA            267..339
                     /locus_tag="IWK70_001354"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="IWK70_001355"
     tRNA            351..422
                     /locus_tag="IWK70_001355"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="IWK70_001356"
     tRNA            439..524
                     /locus_tag="IWK70_001356"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="IWK70_001357"
     tRNA            535..608
                     /locus_tag="IWK70_001357"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="IWK70_001358"
     tRNA            618..691
                     /locus_tag="IWK70_001358"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="IWK70_001359"
     tRNA            711..783
                     /locus_tag="IWK70_001359"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="IWK70_001360"
     tRNA            811..884
                     /locus_tag="IWK70_001360"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="IWK70_001361"
     tRNA            898..971
                     /locus_tag="IWK70_001361"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="IWK70_001362"
     tRNA            995..1084
                     /locus_tag="IWK70_001362"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="IWK70_001363"
     tRNA            1097..1170
                     /locus_tag="IWK70_001363"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="IWK70_001364"
     tRNA            1189..1264
                     /locus_tag="IWK70_001364"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="IWK70_001365"
     tRNA            1266..1336
                     /locus_tag="IWK70_001365"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="IWK70_001366"
     tRNA            1350..1423
                     /locus_tag="IWK70_001366"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="IWK70_001367"
     tRNA            1448..1535
                     /locus_tag="IWK70_001367"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="IWK70_001368"
     tRNA            1557..1628
                     /locus_tag="IWK70_001368"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="IWK70_001369"
     tRNA            1651..1740
                     /locus_tag="IWK70_001369"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="IWK70_001370"
     tRNA            1753..1826
                     /locus_tag="IWK70_001370"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="IWK70_001371"
     tRNA            1845..1917
                     /locus_tag="IWK70_001371"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="IWK70_001372"
     tRNA            1921..1993
                     /locus_tag="IWK70_001372"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="IWK70_001373"
     tRNA            1998..2068
                     /locus_tag="IWK70_001373"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="IWK70_001374"
     tRNA            2081..2154
                     /locus_tag="IWK70_001374"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="IWK70_001375"
     tRNA            2166..2237
                     /locus_tag="IWK70_001375"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     gene            2503..3696
                     /locus_tag="IWK70_001376"
     CDS             2503..3696
                     /locus_tag="IWK70_001376"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9613911.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="IWK70_001377"
     CDS             3826..5298
                     /locus_tag="IWK70_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9613912.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="IWK70_001378"
     CDS             5381..6169
                     /locus_tag="IWK70_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9613913.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="IWK70_001379"
     CDS             complement(6236..6904)
                     /locus_tag="IWK70_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9613914.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="IWK70_001380"
     CDS             7019..8731
                     /locus_tag="IWK70_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613915.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10503
                     /locus_tag="IWK70_001381"
     CDS             8734..10503
                     /locus_tag="IWK70_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613916.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(10708..11274)
                     /locus_tag="IWK70_001382"
     CDS             complement(10708..11274)
                     /locus_tag="IWK70_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9613917.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(11274..12230)
                     /locus_tag="IWK70_001383"
     CDS             complement(11274..12230)
                     /locus_tag="IWK70_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9613918.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(12230..12886)
                     /locus_tag="IWK70_001384"
     CDS             complement(12230..12886)
                     /locus_tag="IWK70_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9613919.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            13256..15274
                     /locus_tag="IWK70_001385"
     CDS             13256..15274
                     /locus_tag="IWK70_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9613920.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            15701..18115
                     /locus_tag="IWK70_001386"
     CDS             15701..18115
                     /locus_tag="IWK70_001386"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9613921.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            18463..20016
                     /locus_tag="IWK70_001387"
     CDS             18463..20016
                     /locus_tag="IWK70_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9613922.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(20397..22181)
                     /locus_tag="IWK70_001388"
     CDS             complement(20397..22181)
                     /locus_tag="IWK70_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613923.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(22196..23917)
                     /locus_tag="IWK70_001389"
     CDS             complement(22196..23917)
                     /locus_tag="IWK70_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9613924.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            24017..24778
                     /locus_tag="IWK70_001390"
     CDS             24017..24778
                     /locus_tag="IWK70_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9613925.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7760 a         4623 c         5355 g         7381 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattatt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtagct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac tgtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcac
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
    10561 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
    10621 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
    10681 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
    10741 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
    10801 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
    10861 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
    10921 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
    10981 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
    11041 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
    11101 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
    11161 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
    11221 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
    11281 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
    11341 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
    11401 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
    11461 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
    11521 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
    11581 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
    11641 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
    11701 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
    11761 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
    11821 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
    11881 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
    11941 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
    12001 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
    12061 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
    12121 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
    12181 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
    12241 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
    12301 acagccaaga aagactgagt aagtaaccgc cgatgatatc agtaggaaaa tgaacgccca
    12361 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
    12421 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
    12481 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
    12541 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
    12601 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
    12661 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
    12721 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
    12781 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
    12841 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
    12901 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
    12961 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
    13021 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
    13081 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
    13141 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
    13201 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
    13261 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
    13321 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
    13381 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
    13441 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
    13501 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
    13561 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
    13621 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
    13681 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
    13741 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
    13801 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
    13861 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
    13921 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
    13981 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
    14041 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
    14101 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
    14161 ggaaaatggc tcttcgacga ataattccgg taacaatacc aattcttcag gaaatgctgg
    14221 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
    14281 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
    14341 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
    14401 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
    14461 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
    14521 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
    14581 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
    14641 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
    14701 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
    14761 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
    14821 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
    14881 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
    14941 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
    15001 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
    15061 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
    15121 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
    15181 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
    15241 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
    15301 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
    15361 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
    15421 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
    15481 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
    15541 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
    15601 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
    15661 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
    15721 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
    15781 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
    15841 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
    15901 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
    15961 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
    16021 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
    16081 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
    16141 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
    16201 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
    16261 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
    16321 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
    16381 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
    16441 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
    16501 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
    16561 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
    16621 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
    16681 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
    16741 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
    16801 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
    16861 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
    16921 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
    16981 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
    17041 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
    17101 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
    17161 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
    17221 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
    17281 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
    17341 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
    17401 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
    17461 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
    17521 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
    17581 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
    17641 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
    17701 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
    17761 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
    17821 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
    17881 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
    17941 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
    18001 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
    18061 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
    18121 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
    18181 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
    18241 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
    18301 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
    18361 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
    18421 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
    18481 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
    18541 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
    18601 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
    18661 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
    18721 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
    18781 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
    18841 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
    18901 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
    18961 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
    19021 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
    19081 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
    19141 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
    19201 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
    19261 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
    19321 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
    19381 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
    19441 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
    19501 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
    19561 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
    19621 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
    19681 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
    19741 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
    19801 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
    19861 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
    19921 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
    19981 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    20041 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    20101 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    20161 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    20221 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    20281 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    20341 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    20401 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    20461 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    20521 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    20581 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    20641 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    20701 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    20761 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    20821 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    20881 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    20941 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    21001 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    21061 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    21121 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    21181 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    21241 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    21301 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    21361 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    21421 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    21481 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    21541 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    21601 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    21661 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    21721 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    21781 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    21841 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    21901 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    21961 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    22021 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    22081 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    22141 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    22201 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    22261 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    22321 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    22381 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    22441 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    22501 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    22561 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    22621 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    22681 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    22741 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    22801 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    22861 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    22921 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    22981 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    23041 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    23101 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    23161 aagcataaac gattcccatg agcaaaaaag caattggagt gacccaagca aatattcggt
    23221 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    23281 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    23341 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    23401 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    23461 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    23521 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    23581 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    23641 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    23701 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    23761 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    23821 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    23881 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    23941 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    24001 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    24061 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    24121 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    24181 tttttataga aaattagata taccgatcaa acagatgaag aatttatatg gaaaaacatt
    24241 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    24301 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    24361 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    24421 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    24481 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    24541 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    24601 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    24661 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    24721 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    24781 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    24841 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    24901 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    24961 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    25021 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    25081 gagaatgata aattaaatag gtgcataaaa gcattacac
//
LOCUS       DACDTO010000033        24674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDTO010000033 DACDTO010000000
VERSION     DACDTO010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.033"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(46..1533)
                     /locus_tag="IWK70_001391"
     CDS             complement(46..1533)
                     /locus_tag="IWK70_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613926.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKGFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITAIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIDNRTSVD"
     gene            complement(2088..2651)
                     /locus_tag="IWK70_001392"
     CDS             complement(2088..2651)
                     /locus_tag="IWK70_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9613927.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(2666..3709)
                     /locus_tag="IWK70_001393"
     CDS             complement(2666..3709)
                     /locus_tag="IWK70_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613928.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(3842..4246)
                     /locus_tag="IWK70_001394"
     CDS             complement(3842..4246)
                     /locus_tag="IWK70_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9613929.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(4479..5114)
                     /locus_tag="IWK70_001395"
     CDS             complement(4479..5114)
                     /locus_tag="IWK70_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9613930.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(5380..6432)
                     /locus_tag="IWK70_001396"
     CDS             complement(5380..6432)
                     /locus_tag="IWK70_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9613931.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(6445..7383)
                     /locus_tag="IWK70_001397"
     CDS             complement(6445..7383)
                     /locus_tag="IWK70_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9613932.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(7834..8484)
                     /locus_tag="IWK70_001398"
     CDS             complement(7834..8484)
                     /locus_tag="IWK70_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613933.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(8584..9093)
                     /locus_tag="IWK70_001399"
     CDS             complement(8584..9093)
                     /locus_tag="IWK70_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9613934.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(9178..9657)
                     /gene="rlmH"
                     /locus_tag="IWK70_001400"
     CDS             complement(9178..9657)
                     /gene="rlmH"
                     /locus_tag="IWK70_001400"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9613935.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            10085..11374
                     /locus_tag="IWK70_001401"
     CDS             10085..11374
                     /locus_tag="IWK70_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9613936.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(11413..12018)
                     /locus_tag="IWK70_001402"
     CDS             complement(11413..12018)
                     /locus_tag="IWK70_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9613937.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(12223..12687)
                     /locus_tag="IWK70_001403"
     CDS             complement(12223..12687)
                     /locus_tag="IWK70_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9613938.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(12709..13029)
                     /locus_tag="IWK70_001404"
     CDS             complement(12709..13029)
                     /locus_tag="IWK70_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9613939.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(13173..14252)
                     /gene="pepA"
                     /locus_tag="IWK70_001405"
     CDS             complement(13173..14252)
                     /gene="pepA"
                     /locus_tag="IWK70_001405"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9613940.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            14452..14802
                     /locus_tag="IWK70_001406"
     CDS             14452..14802
                     /locus_tag="IWK70_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9613941.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            15025..15972
                     /locus_tag="IWK70_001407"
     CDS             15025..15972
                     /locus_tag="IWK70_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9613942.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(16029..17447)
                     /gene="pepV"
                     /locus_tag="IWK70_001408"
     CDS             complement(16029..17447)
                     /gene="pepV"
                     /locus_tag="IWK70_001408"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9613943.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            17596..18441
                     /locus_tag="IWK70_001409"
     CDS             17596..18441
                     /locus_tag="IWK70_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9613944.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            18445..18885
                     /locus_tag="IWK70_001410"
     CDS             18445..18885
                     /locus_tag="IWK70_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9613945.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            18935..19129
                     /locus_tag="IWK70_001411"
     CDS             18935..19129
                     /locus_tag="IWK70_001411"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613946.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            19265..19951
                     /locus_tag="IWK70_001412"
                     /pseudo
     CDS             19265..19951
                     /locus_tag="IWK70_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(20359..21048)
                     /locus_tag="IWK70_001413"
     CDS             complement(20359..21048)
                     /locus_tag="IWK70_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613947.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(21023..23152)
                     /locus_tag="IWK70_001414"
     CDS             complement(21023..23152)
                     /locus_tag="IWK70_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9613948.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(23200..24537)
                     /locus_tag="IWK70_001415"
     CDS             complement(23200..24537)
                     /locus_tag="IWK70_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9613949.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
BASE COUNT         7493 a         5050 c         4311 g         7820 t
ORIGIN      
        1 ggataaatgg aagaaacttc aatgattgtt tctccttgtg ttccttcaat cgacactagt
       61 cctattatca atctctcttt tttgtccatt tacttttgcg actgttcccc aataatcaaa
      121 tacaggaagc gttatttttt gtcctttaga ttcatgtact gtatagacta ttttcgaatc
      181 catcacttga taaggcatag tatgccattc cttgccaatt cttacttcat gattctgcaa
      241 atgttctttg tattgtaatg cttccgtggg tgcatagtca taaagtcctc cgggaaggag
      301 tgtcgtgtaa cgaataagat tttcttgatt cattaccaag tgatctctct gagtgacaac
      361 tttatgttca tagtttgctt ttgttgcagc agatacagcc aacagtaata aaacaattgc
      421 cgttatatta actacttttc tttgcttagc tgatgtgctc ttcatgaaaa cgaacgataa
      481 aatcaacgag ccaaacagta cttgaaagcc taagattcgc caaggaaatt gaatcatttg
      541 aatgggtgta ttttgtaata aagaccatgg aaaaaatttg gtggataaga gccaagagac
      601 taccgcacta aggtaaatca ctttttcttt gcgattaaat cctttaaaaa agaagagtgg
      661 acagatcaaa gaaataatac caatgattcc taccgtaaag gttgtcaggt cattattcat
      721 cgcaccaatc aaactatccg agacatttaa tgcttggtgt tgtaaggtcg ttttatctgg
      781 ctcattaatc gattgaacca tgatttgtcg gaacatcgga taccaaaagt atgctgttaa
      841 aagaatcgtc attcctactg cctttgacaa ctccacaatc aaccggatgc tttctttttt
      901 tcttgtttca cttactacaa agccgcctaa gactagtaag ccaacggcta ttacactcat
      961 aaaagtggaa agaacatgtg tataaatgag taagctcata ctgactgcta atggaattgc
     1021 acatggcttt ttctcataaa tcagtgcgta agcataatac ataactatcg gtataaccgt
     1081 aagtgcaatc gcttcagcaa tagcagaacg atgataaata tcaatcgtac ggtacccata
     1141 aaatgtatat aaacagctaa atataaaagc cgcatcgatt ctttttaaaa attttagtcc
     1201 ataatgaaac atcagcatga tagtacaaac attcaatagc caaacatata agatataact
     1261 aacgattaaa ttattactta tccaataaaa aatgactgcc ggaaaaaagg tcaagtaggg
     1321 ataaaaataa ttcactccgc tcccataatg attaaacgtc gtaaaattga ttggaccaga
     1381 aaaaattgaa gataaccctt tgactctagc aaaatggaag gcaatatccc ccgatgtaga
     1441 aatagtatga acatttctaa aataattgat tgaataaagt ccagcaataa ttgctaggag
     1501 aatggacaat attatttttt tctttacttg cattacttaa ttatatactc ctttcatgga
     1561 ttgctttatc tagtataact taaattttaa ataaatcgtt atttattttt actctcactt
     1621 aaaaaagcac taaaattata agagagggag gtaatggtct gaacaccaag aattgttcca
     1681 acatcgaatt gcttaactaa tgagttcaaa gcttaattat gtggctctta tatttaccga
     1741 gcgtggattg cgatctcttt agcgaaacaa acatctttca taaaaaaaac agccatatcc
     1801 gctttcttgg atatgactat ttactactac tttactatct ttcattcttc ttgtctcact
     1861 tatgataagg cgctgcgtat catgagtgaa tgatattagg aaaacttcat aaaataaatc
     1921 ttttatacat ttccacttct agatggtcat tcatgttaga agaatcaaac tattttttta
     1981 ctgtcgtaat aatattaaaa cactagaaca aatcaattaa attccataaa atataaataa
     2041 tagacctagc atcatctggt ataaaatgga tgctaggtcc gttatattta ctcaaaattg
     2101 attctacgaa tagatcttct tatcatttct attttagtag tcatgcgttg ttgttcttct
     2161 aaaggtggta atggtataag tagtttagat aaatttgtac catttacatt tggttgccca
     2221 gttccgtaag aaattttttc taataacttc caatataatg gcgagtctaa gaagcagtct
     2281 acgtattctg ataataattt ctcaactaac ctaattcgaa tcaagtaaga agcaaaaaca
     2341 gattcctcac ttatttcctt aaccaagaaa ctttttccca tagttcctcc tgtccttgct
     2401 atcaaaatgt cattttcttc caatcttaaa tcaactagtt tagaattact aatatcacag
     2461 taaggcacac tggaccaatt tacacgtccc tcttgtatat ctgtgattct caaaaactta
     2521 acattgcctt gttttttcgc cgaatctgta tagccatatt gaatcttagt agaaatactt
     2581 cctaaataaa cccatagtcg aacatcgcta gatgattttt cttgtcgttc tttatgaaca
     2641 acagtgatca tcttgagttg tccgactatt ttttagttgc agcaatcttt ttgggacgct
     2701 tatttgcatg aagcaacgtt gctaagtcta gtaataaaac attggctaac acaggattgg
     2761 ctttctcgcg atacgctgcg gcgccacttt caaacaatac tgctagacga tcttctacaa
     2821 caacgatttg ttccaaatac ggtggaaatt tgattcgttt taggatccgg ctaggagagt
     2881 cccgctcgga cactaagagt tccgaggtat aaggaccata agaacgactg gctaagagat
     2941 aatggccata aaaagccaca ccttgaatct tatcagatgt ttttctgctc attgtcacgg
     3001 ttggttggat tttgtctttg cggcgaattg ctgttttcat ttcagctatc gtcggcaata
     3061 gatagaaagt caactgtcct tcacctttta gtgtgaagtt gccagatacc agttgatcat
     3121 tgttttgagt caaatacgat gcttgtggaa tctctgagag atcagttgtc agccaataag
     3181 cgattggctg tcccgtctct tcagatgtat ctgctaagat atcgatcaaa cgtaaagcgg
     3241 ctactcgtcc agtagcagat cctgtgattg tcaaccataa aagttcccgt tttgtatcat
     3301 atactaagcc ccctacatgt ggccgtttag gtaaaatcag cagtttgatc ggctgtcctg
     3361 tttctctatc cagtacatga ataatagaat gatgctgatg gtcatagcag taggcagaaa
     3421 taaacacaaa atcttgagaa aaagccaacc cttgcggcgt catccattga caaacctcat
     3481 tttcgccggt ttccggcacc accattcttg tttctgttaa tccaggaatg ttatattgtt
     3541 ttgcttcagc cttttcccaa gctgcttgaa caataggata ccgttttaac tttttttcaa
     3601 tatcctgatg tatccgttgc tgcttccaat gcttaaactt taggacatag taaacaagaa
     3661 ggccaatgac cagcaacact aaaatcatta ttgctacccg ctgtgtcatt tttttgcctc
     3721 cttgttctat tcgatcaatc gtccatatac cgctattaat taccgtaact acatgtttct
     3781 tttactagcg tttctttata ggatctttca taatctcatt tatagtaaca ttaagaatcc
     3841 cttattctac tatagtcact tccacatcaa tattgcctct tgttgcttta gagtatgggc
     3901 aaaatgcgtg ggcttcttct gcgattttct ctgcttggtc cttctcaagt cctggcaaag
     3961 aaacttctaa aacgaccccg atttggtagc ccccatcttc taattgaaat aaagataccc
     4021 gtgctttgac ttcagaatca gactcaagat tattttcttc cagcatgtgt tgaaccgcgc
     4081 cgttgaagca agatgcgtat ccagccgcaa aaagttgttc cggatttgtc ccttcactat
     4141 ggattcctgg gcggtcgatc ttcatgtgca tacttccgtt tggtgcctca acttcgccat
     4201 cacggcctcc acgattgatc attgctgttt cgtacatttt tttcattgag atcatccttt
     4261 ctttcgtcgt tctatattaa gtgtaacgag tattttttaa tctgacaaag gtaatgcttg
     4321 cattcggcga catcagttag acaacatcga tgatcagtga tagaagcagt ttatctatac
     4381 aaaaaaagaa gggttctatg atatttggtg tccttactcg ccttgagtaa caacaccaaa
     4441 tatcatagaa ccacttaatc agcttcacac tgctttttct actttcgtag ttgaaaaaaa
     4501 cttaaacaaa tcatcggcaa atagttaaag aaaaaactta atcgttgaaa gaagcccaat
     4561 tgcttaaaga aggcaaactg actaatcgac ggaatccgtg ctaatccgta aagacctgca
     4621 attagtaaac taataactgt taaaacaaga taaaaatggc taagcgtacc ttgaccactt
     4681 tgacgataaa gcagaacaag taacaatggg aaaagtagaa agcctgcata gcctagccca
     4741 gaacctatat tatggatcca agtcgataga ttacaagagc tttcattcgt attgatactg
     4801 aataagccag taaaaataca atcacctatc ccatataaac caactgttgc aacaacaagt
     4861 attgcaagtg tttttgatgt tccaacaaat aggtgataca acgcaggcaa cgaaagaaca
     4921 aaaaaaagtc cagatacaac tgaccagacc aagaaagcac gtctgactgg actatctacg
     4981 tcaccaaaca cactaattac ggtagtcatt tgattcagtt tgggataaaa aaggccaagg
     5041 atatacggtg tcgataaatc actcatcaca cctaataata aaaaataaaa accatatttt
     5101 tgcaaaaaaa tcattttctt cccctacttt ttagtgatta ttcgatacat ccaacaatca
     5161 aattttttct cattttaaca catattttta ttctagttgt cttttgaaca atcaataaca
     5221 taaaggtgag tttcttctac attttaaaaa atcgaatggc actgaagagt tcagttgtca
     5281 gcatcaatct cctactcaaa aaggcaaaaa aacgtaagtc agatgttgtt gacatctcac
     5341 ttacgcatcc attgattgaa aatttcttag taaaatctac tagcctaaaa atttgaagac
     5401 ttcttctcga tcatacagtt taaatacttt ctcgcataaa aattgagtaa ttagtgccgc
     5461 aaaaacaggg atgactaacc aacaaagaac aacaagaaag ccatttagcc ctgcatctaa
     5521 agaagccaac ggaccaacaa gtccgaccac gccgaaacca gcagaagcag gcgtacctga
     5581 aatagaaaat agagcgacag gaatagctgt aatcactgca ctaactaaac aaggaataag
     5641 aatcacaggt tttctaaata gattaggcat catcatcttc atagcgccta aagcaatcgc
     5701 aattgtcaca cctgacttat taaccttcca agagttcact actaaaacaa aagtggttgc
     5761 agctacgccc atcgcagccg caccagcaga aacaccatct agttggattg ccattccgat
     5821 tgctactgtt gaaataggag agataatcaa aaaggcaaag gaacaaccaa tcaagataga
     5881 cattaacagc ggttgtaagg tagtgaagtt attaatcaat gatccaattg cagaagtaat
     5941 tgctgttaca aaagggaata aataaaaacc aagtaaacct gcacctacac ctacaacaat
     6001 cggtgttaac acaatcgaca ctgacccaaa actatttcca atcagtaaaa tcagcccgac
     6061 agcaatcgat gctgtcagca tcgtattaat aatatcgcct gtaccagcac caacgtaaac
     6121 acccgtttca cccaaatcag gtgtgaattt gactacacca gaaccaacaa aagaagctcc
     6181 ggctacaacc atcattctca tcggatcaaa tccaaattgt aaggcaatca aaccaccaat
     6241 gatcaaaggt gttgctagtt gaaaaataac tgctaactgt gcgatcgtta ccgcaaaggg
     6301 atacgcacta aaatatttta aaatggcacc taatactgca ttaggaatca atccaataat
     6361 gattcctgtt gctgtaccag cgaggacttt attcagaaaa attttcggtg atattttttg
     6421 tgaagtttcc attcaatctc tcctttattt tacgttcaaa ctatcttctt ttgcatggac
     6481 aagctcagtc aagaaatcac aataaggcgt cgcaacacca tatttcttcc cttttctaga
     6541 aattgcgcca ttgatataat cgatttctgt taatcgatgg ttcttaatca agtcttgata
     6601 cattgaagga taatgtaacc caattgtttc cgggtcaaaa caagattcac aatgtgcaat
     6661 gacttctggg acatctagtt caatcttctc tactgctgct actttggcaa actcattgac
     6721 aatcgtcgcc accattttat gagcagtcga tgtttttcct agctcagcca tattaacgtc
     6781 taagattgtg catagtccgt tcattgttcc gttcacacat gctttgcgat aaatagaata
     6841 gtgaatattg tcagaaaaat gagcatttaa tcctgattca gacaatttgt ctgctaattt
     6901 ttttgcagct gcttcttttc catcacctaa attttgtaat tctactgaac cacttccaaa
     6961 taatttgacc tgaccaggac cttcaagacc tgccgtccac atggtatttc caatatagat
     7021 attttccatt ggtacaaatt tctcaataat atcttcatgc ccgataccat ttaatagaca
     7081 taagacctct gtatcttttt tgattaatga ttggatatcc tgcagcattt tttccagctg
     7141 catcgctttg gtaaatagaa taatcagatc aacttgatct tctttttcta cttcggattg
     7201 aagaacgatt ggtagttttg cttctacctc tttaccatta aaatttgctt gtaatccatg
     7261 ttctttgatt tgttggacat gttctgccca gccatcgatt aataatactt cgttcccact
     7321 ttggtgaagc atcaacccaa agcgacttcc cattgcgcca gcgcctgcaa ttgctatttt
     7381 cattgattct tcctcctttt tctttctatt atactcattt gagaatcttt tcacaacctc
     7441 gttttgttat tcttctctta tttatcatgt ttatatccac cattcaagtt attttatcag
     7501 caaatcaata aaaactggaa tagaatccca atttcatcgt aagagaaact agactagcaa
     7561 taagaaaata gaaacattca tagaaaaaat ctcactcctc tgattataaa agagggggca
     7621 ttaacctcgt gtatactaaa accttccatt cattcgatgg atgattttct ttttgtgaaa
     7681 aaatattcat tgtagtacga tgttgcgcaa cacagacgga tggtattgct tattatttat
     7741 taaaatcgaa caatacactt atttaaaaac ttctttccca gattttcttc catttttaca
     7801 gttataacta gtattatcat gaaaaatagc cttttattgt ctatgggaat aataatttaa
     7861 aattggtact tttttattaa ttagttcatc gcccaataat ctctccagtt tcttagcatc
     7921 tcctgactca accggaatta tccaagccca gtctaaataa ttttttaaaa tctcatcata
     7981 ttttgcaaat atttcattgc catattccgt ccttaaaatt atattttcag tgccagacaa
     8041 gtgatttata ttgagttgat aaaaaagatc tccatctaaa aaaatatcat tagaagaaga
     8101 tttccccaca gtaatgacca ttccatcagc tctatgtgtt acatacaaat aattgtatga
     8161 cctcatttca cctaatgaat caattatttc ttgcccttta gctctaatat attgatctct
     8221 attacctatc tcactcttat ttaacaaaag ttttttcatg tcaaatttaa tcggctgttt
     8281 tatttttgta aattcgcttc tactactaat ccaatccgag attaattttt caacatgtcc
     8341 cagagtaaac tctgatttat tcgctaaata ctttttttgt tgtattaaag acatctttcc
     8401 ccgaagctca aaattgagcc tacatcgatt ctctatctct tcaaaaagca tcttatcttc
     8461 atcattctta tattcatcat tcataaaacc aatccagctc ctcgattaag ataatatttt
     8521 aatctcatac aaaaactagc tctcatcaaa acaaccattg ttttgatttc tcatgaaatt
     8581 tctttatact tttctccgat tataaattcc taaccccttg agaaaattac gcatatattt
     8641 atctccgcaa ggcttaaaat ttcgttggcc ttcttttctc aaaaatgccc cgatttcgcc
     8701 ttttgatgga tagatttctg cctcagcaaa aacgtctaaa atatcatcag tggtcataga
     8761 caaggcgatt tttaattttt ttagcaacac attgttgata tatttgattt ccttgctttt
     8821 ttgcggcgtg tctactatcg gcttatcttt ttgcggacca cgcgccaaga gaatcaggcc
     8881 atttaggaat gcgtcaaaaa cttggttgtt gatggttttt tcatacacat tattttctgc
     8941 attatctttg ttcttatcct gtactttggt cagtaccgcc tgagcttctt ctttagtcag
     9001 tggtacaccg cctagctcaa aagctttaat caaatccgta tctttcaaat caatcgcata
     9061 acgcaatcgc aataacctat cattgttatt catttgttcc tccgaagtat ttagtcttta
     9121 ctttcttacg tactttttgt cttgctaaga tttttaggat caatgataaa aaccgcatca
     9181 cttatgataa ggcgcccccg cattgatccg gaaggcacgg tagatttgct cgacaaggat
     9241 cagacgcatc aattgatgtg gatacgtcat ttttccaaaa gagatctgag cattgcttct
     9301 tttcatgacg gcttcgctga gtcctaaaga gccgccgata acaaaaacaa ggtggctttt
     9361 tccttggata cctaattggt cgatttgttt agcaaaagat tcacttgtag gatttttccc
     9421 ttcaattgct aaggcataga cgtattcttg ttctttgatt ttcgctaaga tccgttcccc
     9481 ttctttttct tttacttgtc tcatttgcgc ttcgcttaaa ttctccgggg ctttttcgtc
     9541 aggaacttcg atcaattcga tttttgcata ggcattcagc cgtttgacat actcattgat
     9601 accttggatc aggtattttt cttttaattt tcctactgag ataattttga tattcatgtt
     9661 ttctctcctt tggtcttcat gctcactttg tttattataa tcaatttttt cgtctatatg
     9721 tttattttgg ctatttttgt taaaaagatt tccccaacag ttatgcacaa catccacagg
     9781 tttatccaca gaaaacagtg ttattttacc ttatttcatc acattcatta ggcaaacata
     9841 cgttttataa gaatttatcc actttataca caggctgtgg atatgtttta tcccttattt
     9901 ttccaatcct atccgcgtag ttttacacag tatctgtgga aaaagaaaat caaatatttt
     9961 catctgaaaa caatgatttt tcctaaagtt tttcataaaa agttcacagt ctcaaagtag
    10021 actaaagtca acaaataaga ctatactaac agtagataaa tgaatgtaat tggaggagga
    10081 acacatggat agaaaaaatg tgacaccaaa gatgaaaaaa agtaaaaata atagcctctg
    10141 gcgtaagtta ggtctggggc ttgttggagg gatcgttgga ggactactga cagctggcat
    10201 tttttatgca gcgatgggca ctggcaacaa tagttctact gctacaagcg gcaatcagaa
    10261 ttctgccggt gaaacagttg tggaaaatgt caaagtcaat gtagattctg atatcaccaa
    10321 agcggtagat aaagttcaag acgctgtcgt ttctgttatc aacttacaaa gtcaaaacca
    10381 atcaagcggt tttggcggac tattcgggca gcaagaagaa agtagcagct cagatgatag
    10441 taatttggaa gcttatagtg aaggaagcgg cgtgatctac aaaaaagacg ggaatactgc
    10501 ttatgtagtc acgaataatc acgtagtaga tggccagcaa ggtcttgaag tcttgatgaa
    10561 agacggtacg aaggtaaaag ctgaattagt tggaacagat gcttattcag atttagcggt
    10621 gttgaagatc aattcagaca aagtcgaaac agttgcctct tttggtgact caagtgctct
    10681 gaaggttggt gagcctgcga ttgcgattgg ttccccacta ggatctgaat atgcaaattc
    10741 tgtcacttca ggaatcattt cttccttgaa ccgacaagtg acaagtacga atgaatcgaa
    10801 cgaaacagtc aatatcaatg cgatccaaac agatgcagca atcaaccctg gaaattctgg
    10861 tggtccttta gttaatatcg aaggacaagt tatcggtatc aactcaagta agatcgcaag
    10921 tacttctgaa tctacttcta atgtaagcgt agaaggaatg ggatttgcta ttccaagtaa
    10981 cgatgtagtg aatatcatca atcaactgga aaaagacgga aaagtgacac gtccagctct
    11041 aggtatcaca atggtcgact tatctgccgt ttctactcag caacaagaac aaatcttgaa
    11101 gattccttca tctgtaacta acggtgtcat cgtgacaaat gtccaaacag ctacaccggc
    11161 tgaaaaagct ggattgaaac aatacgacgt cattactaag atcgatgaca aagaagtttc
    11221 ttctggtgta gaacttcaat ctgctttgta taagaaaaaa gtgggagatt ctgtcaaaat
    11281 cacttactat gaaggctcga aagaaaaaac aactactgtt gaattgacga tcgatcaatc
    11341 ggcattaaaa caaaatcaaa atagtgaaaa ttaataaaaa agaggttgtg acaaagactt
    11401 gtcacaacct ctttattttg gaaatgcttc tccgactacg gcatcctcag gcaatactaa
    11461 gattcctttt tttgctggag catttggcaa atgcaattca cgagcggaac agatcattcc
    11521 atagctatct acccctcgca aagttcctgg ccagatcatc attccatctg gcatcatagc
    11581 tcctggtttt gcaacgacaa ctttttgtcc agcttcgata ttgggtgcac cgcaaacgat
    11641 ttgcaatact gtaccattgt ctacttctgt ctcagtaatc gataaatggt cagaatcagg
    11701 atgtgggacg catgttttta cgtacccgac aacgatcttc ggttcatgat cagcttcaag
    11761 ctgttcttcg aatcctgctt ctttgatcaa tcgattcaat ttcgctaatt cctcatctgt
    11821 taagcttact tggccgttcc cttcgattgt tagatgatcg gagatatgga aaaagttcca
    11881 agcaacaact tgaccttgtt catctttgat ccgagcaaca ttccatttgc gttcaacagt
    11941 gttttctgtt cctttatcgt cggctataat aaccatcaag gtgtctccga cgtgtgcttt
    12001 gttataagca aaaatcatgt tctttcctcc attatccttt gctgagattt atcttaacaa
    12061 aaaattgcat tcactgcatg aaattttttt catgctttat ttccgaagaa gcctcttctg
    12121 gaacaccatt tattcgtaac aagggactgt gacaagactt ttgtcacagt ccctttactg
    12181 ttttagttaa ttgattaaaa aggtcgtaca agttccactt gcttaattac ttggctgttc
    12241 ttcttcgtca gaaacgatca agacatcaca aggtgaatga cgtatcacgt agctggctac
    12301 acttcctgtc ataaagcgtt cgactgcatt tagacctgac tgcccaacca tgattagatc
    12361 gatctcatat ttttttggca atttgactgc catggattca cgaacagaac catatgcaat
    12421 aattccttct atatttgtgt aatcaacaga tttcccatat tctttacatt cttcgatcat
    12481 ttccttggct gtttcagttt gctgatcgac tagattttga tcaataggtg catagcccat
    12541 aaagttataa aattgctgat cgattacgtt tgctacatat actttcccat tattacggcg
    12601 agccacttct accgcttttt tgaaagcagc aagtgcttgt tcactaccgt cgatacctac
    12661 taaaatattc ttatattctt gattcatctt gacccctcct aacagatgtc agccttttgt
    12721 tacactttct aggaacgatt cgatttcctg ttttgtttta cgatctttat tcactagacg
    12781 acctaattct tttccgtctt ggatcacgac aaaacttggg ataccaaaaa tgttccattc
    12841 agcggctaca tctatgtact tgtcacggtc tacttcaata aattgccaat cagaaaagtc
    12901 attttcaatt tcaggcattt gcggtttgat aaaacggcag tctccgcacc agccggcagt
    12961 aaagaaaaat acactctttc cagtttctac gtagcctgct aattcttcta aactatttgg
    13021 gataatcatt ctttgtcctc tactttctgt ttatgtattt ttcaattaaa ttatacacct
    13081 ttctttcgaa ctaacaaaca ttgatcctct ttttataaaa aaaatctggc aaaaagcaat
    13141 cttttgccag attttatagt ttcactatcg cattattttc cgtatacgat cgtatttact
    13201 gttgatttat ccaaaccttt cagtgtttga atcaacattt ctcgtgcagc ttcaaaatcg
    13261 cgaatactga acatcgtttg atgcgtatgg atataacgtc ccactacgcc aatcactgta
    13321 cttgggattc cttcgttttg tgtatgagca gcacctgcat ctgtaccgcc ttttgaaaca
    13381 aagtattgat aagggatatt gtttgtttca gcgatatcca gcaagtactc gcgcaaacga
    13441 ggaagcatga tcaaacctgg atcttggata cgcatcaatg taccttctcc taaatgtccg
    13501 aatgtgccat ttttcgtcac tgtatcatct gctgcagagc agtctactgc aaagaacaga
    13561 tctggcttga atttattgac agaagcttta gaaccacgca agcctacttc ttcctggaca
    13621 ttcgcgcctg caatcaatgt atgacctaat gtttcatttt gcaacgcttc taaagcttct
    13681 aataccattg tacagccata gcggttgtcc catgatttac tgataatatt ctttccgttg
    13741 gctgttttga tcgtttcagc gtaaggaacg attgtgtctc ctggaaggac accgtaactc
    13801 attgcctctt ctcttgattc aaacccagca tcgaacaaaa tatcggttac ttctactgat
    13861 ttttgtccgc ttgttcctcg caataaatgt gggggaacgg aagaagaaat acatggataa
    13921 ttcttaccag tagaggtttt aagtgtaaaa cgctgagcag aaacaacgta aggattccag
    13981 ccgcctagag gtacgacttt aaaaagcccg ttttcttgga tttgtgtcag catgaatccg
    14041 acttcatcca tatgtgctgc gaccattaca cggggtgcat ccgcttcttc atgatggcgc
    14101 agaccaaaaa tgccgcccaa accatctaac tgtaactcat ctaccagcgg ttccatatga
    14161 ctttccatat aagcgcgaac atcttgttca aaaccgcttg ttccttgtaa ttctgttaac
    14221 tctttgattc gttgaaatgt tttttcttcc atatgctatt cgtctaaatt tcagactaaa
    14281 ctcctctcag acttatattg atctgcggat aaaatatgat tttttctgca gaccgatcgt
    14341 atttattata gcttattttt tctggtagcg gttatttatt tcaaagaaaa tatgagagtg
    14401 tggtaaaata agcacaagga gtaacaagtg agaagaagga ggtatgtcca catgtatgaa
    14461 gaaagcgaac taagctattt taaaggcggg ttagcagttg gaatcggtct aggattattg
    14521 agcggggtag cttcaacgat ttggtatcat cgcaaaaaag tattgtcagc agaccaagta
    14581 cttgaaaata tcaagtcatc ttttctaaaa gaaggtgcaa tcgaaggttc ttggatctct
    14641 tttgaaaagg aacctattag gaaattcgcc gttcggtcga gtgcttatcg gggaggtctt
    14701 tctcgaatag aagatggcga aactgtttat tatgaatttt tagcagatgc tcatacagga
    14761 accgtattag aaatttcaag aaaaaaaggt gcaacagatt aatacgtcaa aaaagtgaga
    14821 gaacgaatac gcacccacta ttcaaaagag aggtatcatg tccttctttt gtagtgggtg
    14881 gttttttaga tagagctttc caaacatata ttccagtatt ttctttttaa ataaatgata
    14941 tttagcggga aaaactagca tttcctttga ttatgaggta tttttaattt agcttttgtt
    15001 ttctatagtt aaggagagtg aaaaatggaa caacaagaaa aaattgctgt tctgattcct
    15061 tgttacaatg aggaagcaac aatttcaaca gtcattgcag actttaaacg agaattacca
    15121 gaagcagata tttatgtcta tgataataat tcgaccgatc gaacatacga attggccgtt
    15181 caaggtggag caatcgtaaa aaaagaacct cgtcaaggaa aaggaaatgt gatccgccaa
    15241 atgttctttg atatcgatgc cgactattat ttaatggtag atggcgatga tacttatcct
    15301 gcagaagctg tacatggttt gctcgagaaa ttacgttctg gtgaagcaga tatggtcatt
    15361 ggtgatcgcc tatcaaatgg aacctacttt gatgagaata agagaccttt ccacgatttt
    15421 ggaaataacc ttgttagaaa tacaattact agaatgtata aaacaaaaat tcttgatgtc
    15481 atgaccggct atcgaggatt caatcgtatc tttgtcaaaa gttttcctat tatgagttct
    15541 ggttttcaaa tcgaaacgga attgacgatc catgctttag ataaaaaatt caagttcgtt
    15601 gaaatcccaa tcgattatcg tgatcgtcct gaaggcagcg aatcaaaatt gaatacattc
    15661 tctgacggat tcaaagtgat catgatgatt attaaaatgt ttaaagatta taagcctttg
    15721 cttttctttg ggatttggac catcttcttc tttttatttg gattggtcac ggggattcct
    15781 gtgattcgcg aatatatgct gacaagcttt attacacgca tcccttctgc gattttaagt
    15841 actggattga tgactttagc acttctttca ttagtaacag ggttgatttt ggatacagtc
    15901 gtcacgaacg ctaagaaaga atatgaattg aatctatatc atgtttatca tgaataccac
    15961 agtagaaagt aaaaaaagga tcaagactgc aaaaataatt tgcaattctt gatcctcttt
    16021 ttaattaatt atttcgccaa acgatagatg gcatctgcat agattgctgt tgctctgaac
    16081 aagtcatcaa gactcataaa ttcgtttgct tgatgcattg tatcagtata tcctgggaac
    16141 atcgctccat aagcaacgcc acgtttcaac aaacgtccgt aagttccgcc accgatgatt
    16201 tgttcgtagc ctttttctcc agtgtggtct tcatagactt gcaataatgt agccaccaat
    16261 ggatcatcca ttggtacgta atgtggttcc attacgcgag cacctcgagt aactgttgcg
    16321 ccttcttgcc ccactgtttg ttcgatgtca ctttgcaatc cgtctactgt tacacctttt
    16381 ggaaaacgga aattcatatt gatgaagttc gcttcctctg taccattttc ttcaaaagca
    16441 aatagtccag cattcattgt caatttgccc attttttcat cttcataagc gactcctagt
    16501 ttttctccat aaaaatcttc atggacataa gcagctgcta catggataaa ttgtttagca
    16561 gatcctagga attcatagtt atctaagaac aatgccaaga aagaacctgc attgatacca
    16621 gattgtgggc ttgcaccatg tgcgccttta ccaactaatt cgatttttac atacgtattg
    16681 tccgcttcaa tcgttccgct tactggattt gcttcgatga attggtaaaa tgcttcttcc
    16741 atcgcgatag ctgcatctgc acttggtact tgaagtgctg cagttgctgt tcctggcacc
    16801 atattttcgc gaagtccgga aacaaaagat ttcaagacat aatctcctgc attgtcgcct
    16861 ttgaagcgta aagctaaaga aacatttcct ttttcaccat tgatgattgg gaattcagca
    16921 tctggtgaga aaccaaaatc tggttcttct tcatgttcga aataataagc catatcgccc
    16981 cagccgcttt cttcatcgct gccgactacg aagcgaactt tttttgatag tttcaagtca
    17041 agttctttaa tgatcttcat cgcatagtaa gctgccatac ttggaccttt gtcatcgctt
    17101 gatccgcgtg caaagatctt tccatccttg attactggtt cataaggatc tgtgtcccag
    17161 ccatctcctg caggaacgac atccatgtgt ccaaatatcc ctagcgtttc gtctccttcg
    17221 ccaagatcaa tatgccctgc atagttatcg acatttttta ctttaaatcc atcacgttcg
    17281 ccataagcta acatatgttt caatgcgtcg cgaggtccag gtccaaatgg tgcatctggt
    17341 gttacttttg agtcatcgcg ctcgctgttg acacgtaaaa gatttttcaa gtcttcaagc
    17401 aagtcttctt tacgtgcttc tacttctttt tgccaatcaa ttgtcataaa gttaatcctc
    17461 cacttattta agtcttcgtg ttcatgatac cacaaattac agaaaaaaat agttcaaaaa
    17521 atctgacaat tccttttctt atctgttttt ctctaaaaaa aatatacgct ataatgagta
    17581 aaaggaggtt tttagatgct gataaacgaa caactagtga aaagagtcat cacacctcgt
    17641 ccaaaaattt cacataaagg aacatttgga cgtatcgttt tgattggcgg caatgaacaa
    17701 tatggcggag caatcattat gagtgcttta gctgccgtac acagcggagc gggtctcacg
    17761 actgtagtaa ctgatgaaaa aaatcggagt gctcttcaca gtcatctgcc agaagcaatg
    17821 attgtagttt ggacggatac tgaccagttt ctttccgtaa taaagcaagc agatgtactc
    17881 ttgattggtc ccggattggg tacttcttct aagagtttgg aacgtttcaa acaagtacta
    17941 gaaaaccaat caaaagaaca atggctggtg attgatggct ctgctttaag tttacttgct
    18001 gctaatcatc ttttgctacc ctatcccgaa caaactgtac ttacacctca tcaaatggag
    18061 tggcaacggt tgagttctct accgatacaa gaacaacggg ataaagaaaa caaaaagaag
    18121 caacaggaaa tcggtgctgt gctcgttttg aaaagtcatg agacaaaaat ttatggtcac
    18181 cagcacttct acaccaatcc tttaggttct gctgctatgg caacgggcgg catgggtgac
    18241 actttagctg ggatgatcac aggattttta gcccagtttc ctcaaaatga ccaaacgatt
    18301 gcagcagcta tttatcttca tagtttcatt ggagaccagc tggcaaaaga acaatatatt
    18361 gttttgccga cacaaatcag cgaagcactt ccgaaatgga tgaattattt ttgtaaaaaa
    18421 tcaatggaaa acgaggcatg agtcatggag aaatttacag gttatgtgcg agaacctttt
    18481 tattatgaaa cagatcaaat ggggattatt catcacagca attatatccg ctggtttgaa
    18541 gaggctcggg tagccttatt ggtacatcta gggtttccct ataaaaaaat cgaagaatct
    18601 gggatcatca tccccgttct ggaagtaaat tgcagctata aagaaatgat ccattacgga
    18661 gaaaaagtga gcatccatcc aactatccaa aagtatacgg ggacacggtt ggattttagc
    18721 tacgaaattg tgggaactga agacaaaaaa ttaaaaacta ctggcagtag taaacactgt
    18781 tttttacttt ctgattcgca acgattagtg aagctgagca aagtcaatcc ggagttagat
    18841 cagttattcc aagcgtacgc ttctttggat gatgtaaatt cttaataaag accgtctaag
    18901 catttttctt gtattgtgtc gagtagttcg tataatgaaa agaaaaaagc aggtgatccc
    18961 catgtatgtc gttaaagtaa tgcacggtta catcgataaa actggctgtc gcactcggga
    19021 aaaaaactta gacaatctgc tcattttcaa agacaaaaaa gaatccgaag cttttgctaa
    19081 aagaatcggc ggtcgtgtca aaccgatcca agaggtacga ccagattgat tcaatatttt
    19141 tcttttcgag gctctaatag gcatgcctat cagggctttt tttgcagact gtgtttttat
    19201 gcccacacga tttgtaagcg atttatgtta taatagatta tatctgcata gaaaggaagg
    19261 ttaaatggtt atcaataatt tgcaacagca agcttatgaa tctatacgta aacaaatcat
    19321 ttattccgag ttagcacctg gaagtaaaat ctccgataaa gtgttagaag aacaactagc
    19381 tatcgggcgt acaccgattc gagaagcact gatccaactg cgcaatcaag agcttgtaaa
    19441 aaccattcca cagtccggca cttatgtttc aaaaatcgat atccgatctg cttcattagc
    19501 ccgttatacg cgggaaaagt tggaaaaacc gatcctgcaa gaatgcagtg cgaaaatgac
    19561 caagtaagat caagctaaac tagaaaaaat actagaacaa accgatcagg cagtaatcgc
    19621 tcaaaataaa aaagcttttt ttgatttgga tactgatttt caccgaactt gttatgaaat
    19681 tgctggaaaa cgagaaattt gggattggct agaaagttat agtacgcact tgaaccgttt
    19741 cagatggtta cgtttgacga tttcagaatt agactggggt cgagtattag atgaacatca
    19801 gacaatgctt caatcaatga ttgatcataa ctttgatgaa gtcggatttc tttgcacgat
    19861 gcatttacat atgattatcg aggagcaaga atacgtgatt cataattacc ccgattattt
    19921 tgaagatatt caggacagac ccatcaaata agaggttttg gaatcagcgt tttgacctga
    19981 gtattagaga aagaaacggc aaaatagttc ctcatatttt gccgtttttt ttgagctaat
    20041 accgagggtc agctgattgc acaccgttta ttgagaggtt gtgaaaaagc tgccctgcat
    20101 caagtaataa gaacagccaa gtaaaaatag cttcttatgt ttttaacttg gctggactta
    20161 ttaccgcaga tgcaagcttt tgaacaccgt tttccaagag gtcgtgagcc tgccgttcat
    20221 ctccgagtca caaggaacaa tttcaaaaaa cagcttctca tgtttgttga atattttgac
    20281 ttgtgaccga ggagttgaca ggagaacacc gtttattcgg aaaaaaagga ctgtgacacg
    20341 acttttgtca cagtctcttt atttgtaatc taaatcatac tccgtacatc ttttttctat
    20401 tttttttatt tcttctaaga tcccttggct acgaatttca ttatatggcc aaagtaagac
    20461 tgcctctaat acataaggcg aaaaatcgct ttgattcatc tcttttgaaa aactcatcgc
    20521 tgctatctcg ctcaatgcac gaatgtttga ttgatgtgta taaaaaggca accgccacag
    20581 cacaattttt tcgctttgcg tataaattcc cttaacagaa gcttcgatgt ggtgaaatag
    20641 ttcatgtcca ataatcaaag cttgcgctgt ttctttagag atcgaagcta gtcctgaaca
    20701 ttttaatttt tctaacggct ccgtcatcaa tcgaatccct tcatcttctg cataagttgc
    20761 aaaagtgatt cgttcgttta tttgctctga ttcacaggaa acgattggaa cagccatttg
    20821 ttcacaaatt tctcctacat cggcaaaagg gtactttttt ttgatgctac atgccgtttc
    20881 gataccacaa tcgattgatt gttgtataag aattttctgc ttggacaaag gaatcttttt
    20941 ccaaaataaa tctcgccgaa actggtagaa tccccaaatt tcttcgggga tctgttgtaa
    21001 ttcgtttagt aactcttcta acttatgttt gtttgatatt tccggcaacg acgatcactt
    21061 cttcctccgg ttccatagta gatacttcca gatccatcaa aagctgtagt tgttccagat
    21121 ccgtcgctgt aaaatcagag actcttgctc ctaagcagag ataaaattct ggtcgggcaa
    21181 tcaattcagt ttgataaact gccatatctt cccaaagttg ttcgacagct gtcatataat
    21241 tggctgcagt tgttttcata catgaagcat gtccacgttg gaccttgata aatccttttt
    21301 gatcgatgat ccgaagactt ccagcatttt ttgaacgatt ctgttcgccg catacataaa
    21361 agaaaggtgt attttccaat aagcaaactt catttttatt taatcgcata tcctctgcag
    21421 caagctctag tgcttcttct gttgtgatct ccttggttag atcggttgct ttcacttccg
    21481 tagaaccagt agcgatagcc gttacttttg aagtctgtgg atcgatttct acgtggactt
    21541 cgatcgattc tggtgtagcg cctgattcaa tcgctttatt catcgcttca tttttcaacg
    21601 atcgtatatc ttctttactt ggcgaaggga tgatacgttc tacaacatct cggaccattg
    21661 caagtgctac accaatagaa gaaatcactt cggcgttttc aggaatcgaa tatttcacac
    21721 ccattttatt actaaagtaa gtaatcaatg aggcagcgcc gccccctaca cctacaagag
    21781 aaatctgatc tttctccaag tgatacttgt ctgccaattc taaaatcacc ggttcaatct
    21841 tagcatagga tttttccatg atctgttctg cgatgtcttc aaccgtcgta tgacaataat
    21901 cagctaatgc ttggatggct tttcgtgcag atgggacatt tccataagaa aaatgttctt
    21961 cttgcactaa tccaaggaca ttggctgcac atgtatttgt aatagtcact tcttctccgt
    22021 cttccatcac taccttgacg taatcagcag ggtctccttc tttaggagag aaaaattcaa
    22081 ccttaggacc tttgattttt tctgtctccg taaatactgc ataatctaga ccggcaatat
    22141 gtgcagaacg tggaccgaca tcgatgattc ctgactgatt agcccgtacc atcgaaccgc
    22201 ctgccacacc tagaacccga acatctaatg aagagatata tgttgggtgg ccacctacaa
    22261 ttgaataatc gattgctgga cgtccatttt taatgacccc gatattggtt gttgttccgc
    22321 ctacttcaaa ataaacacca ttagacgctc tcaagtacat caaagagccc atgactgatg
    22381 ctgctggacc ggaaagcatg gttaaaacag gtcgtttttt catttcattg atttccatta
    22441 ctccgccgtc tccgcgcata atcatcaaag aaacattgac ccctgcttct cgtacggaat
    22501 cttctgtaga ggttgccgta tctagcattt ttggtaaaat agatgcattg attgctgctg
    22561 tccgagttct tcttgtcaat ccatataatt ttgtgatgtc agaagccatc gttgtaggga
    22621 tcgattttac cgatgctgct tcataaacaa ctcgttctgg ttccccatta tctactccaa
    22681 atgccataga agaaaccaag acctccgctc tttctctttc taaagaagaa atcgtttctg
    22741 aaacacggtc tacatttaag tgtttgacag gtaagaatgc attgacaatc tctatttttt
    22801 tcttattccc taaatcgata tcgttcaatc tagtttgtcg ttttgctaaa aaaccttcta
    22861 atccaccttt tgccatcccg attacgccga cttttgctac gtctccttca atcagtgcat
    22921 tagtcgcttg tgtggtagaa tgtgcaacaa acacaacatc ttctggactg atattatttt
    22981 ccagcaaaca attttgaaaa gactgaacga cacctgcggc tactccacgg acatcatcgt
    23041 gggttgtttt cactgaagat tttccgatga tttcgtgcgt cgcattatcg attgcgactg
    23101 ctttcgtatg tgtaccgcca acatcaattc ccattcgcac aaaacgtttc attttctttt
    23161 cctccattca tctaatcgtt tttatcgacg ttttgtttac taggcgttcc cgtacaataa
    23221 gaacgtgatc aggcataaga tcgctccgat gatccaacca gttgggatgg atttcttcat
    23281 atattctcga gaagttactt ttgtataacc taatccccaa gcaacccaag attgtgtaat
    23341 atctaaatgc tgaggagcaa tcgttgtgat tgcaaataac gggaacaaaa aggctggcga
    23401 catcgttgga ttaacagcta atacgacagc taaaatagct gagcctgaac cgactagatt
    23461 cattggaccg cggaaaaatc ctagtggtgt caatatagca aatacgattg ccaagactaa
    23521 tggtgattgc ggtatccaat ctccaaaaat agctgaaaaa taaggagaag catacgcagc
    23581 tgaattattg aacatcgcta aggtcatcaa gaaaccaacc attggcgcaa catctactgc
    23641 gccatctgta aataatttcg caaactgacg acagatttct ttataacctc cgcgcaattt
    23701 tccagtaaac agcaatgccc agatacctgc tagaataaaa cctaagatga ttggaagatc
    23761 taaaagaacg acccctaaaa ctggtaataa tacggctaac catgagatca ttggtgcgtc
    23821 gccaccttca gctggtacgt ttgctgccat tgcatagcgt ttctttttgt tcattgatat
    23881 attagcgacc gttagtacca caactaagct tacaatcatc ccaatcatac caatttggaa
    23941 atagtcatta taagtatagc tttcagccgc tggattgaaa ccagcataaa tcgtttgata
    24001 ttgtttgaaa ttgacgatat tagcaaagat tccagccata atcgagccca taaatgagaa
    24061 cagtgcaacc ggtactgaaa tacctaatga catcaagatt ggcaacacaa taactgcaat
    24121 agaaatcact ggtccgattc ctgtcatcga catgaatata acagcggtta caatacatag
    24181 taagctcatc gtgattcttg gcttgtcccc tccaagttca actacctttc gaatcagggt
    24241 agctgcgatt ccgctatcta ctagaacacg cccgaaaaag gcaccaaaga agacatttac
    24301 caagatcgat ttggcgtact cagctggccc cgtcgtatag atatgcgtca acacatcaat
    24361 tactccttgc ccttccatcg ccgggtttgg tgaaaacgtg tttccgacta gcgctaatcc
    24421 catccataca gtagccatga tggcaaagcc gaccatcaag ttgtagcctt ttacacagta
    24481 ccagaccatg aaaaagaatg agataactaa caaaatacca ataataatat cactcatgtt
    24541 attcctccgt ccattttgtt tttctgataa gcaaactaat atatcagtca aactcattct
    24601 aacttatgta gttagcgctt tcattaaagg gtgaccataa atgttcagtt ttcaattaaa
    24661 aaaccttaat tatg
//
LOCUS       DACDTO010000034        24373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDTO010000034 DACDTO010000000
VERSION     DACDTO010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.034"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..803)
                     /locus_tag="IWK70_001416"
     CDS             complement(<1..803)
                     /locus_tag="IWK70_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9613950.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKV"
     gene            complement(1027..2967)
                     /gene="thrS"
                     /locus_tag="IWK70_001417"
     CDS             complement(1027..2967)
                     /gene="thrS"
                     /locus_tag="IWK70_001417"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9613951.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3304..3483)
                     /locus_tag="IWK70_001418"
     CDS             complement(3304..3483)
                     /locus_tag="IWK70_001418"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9613952.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3530..5353)
                     /gene="cadA"
                     /locus_tag="IWK70_001419"
     CDS             complement(3530..5353)
                     /gene="cadA"
                     /locus_tag="IWK70_001419"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9613953.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5631..7910
                     /locus_tag="IWK70_001420"
     CDS             5631..7910
                     /locus_tag="IWK70_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9613954.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8003..8650)
                     /locus_tag="IWK70_001421"
     CDS             complement(8003..8650)
                     /locus_tag="IWK70_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9613955.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8829..9548)
                     /locus_tag="IWK70_001422"
     CDS             complement(8829..9548)
                     /locus_tag="IWK70_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9613956.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9679..10830)
                     /locus_tag="IWK70_001423"
     CDS             complement(9679..10830)
                     /locus_tag="IWK70_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9613957.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11047..11787)
                     /locus_tag="IWK70_001424"
     CDS             complement(11047..11787)
                     /locus_tag="IWK70_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9613958.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11791..12114)
                     /gene="rsfS"
                     /locus_tag="IWK70_001425"
     CDS             complement(11791..12114)
                     /gene="rsfS"
                     /locus_tag="IWK70_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9613959.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12171..12764)
                     /locus_tag="IWK70_001426"
     CDS             complement(12171..12764)
                     /locus_tag="IWK70_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9613960.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12748..13395)
                     /locus_tag="IWK70_001427"
     CDS             complement(12748..13395)
                     /locus_tag="IWK70_001427"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9613961.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13408..13719)
                     /gene="yhbY"
                     /locus_tag="IWK70_001428"
     CDS             complement(13408..13719)
                     /gene="yhbY"
                     /locus_tag="IWK70_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9613962.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13735..14844)
                     /gene="yqeH"
                     /locus_tag="IWK70_001429"
     CDS             complement(13735..14844)
                     /gene="yqeH"
                     /locus_tag="IWK70_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9613963.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14841..15368)
                     /locus_tag="IWK70_001430"
     CDS             complement(14841..15368)
                     /locus_tag="IWK70_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9613964.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15548..16333)
                     /locus_tag="IWK70_001431"
     CDS             complement(15548..16333)
                     /locus_tag="IWK70_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9613965.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16326..17195)
                     /locus_tag="IWK70_001432"
     CDS             complement(16326..17195)
                     /locus_tag="IWK70_001432"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9613966.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17196..18575)
                     /locus_tag="IWK70_001433"
     CDS             complement(17196..18575)
                     /locus_tag="IWK70_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9613967.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18583..19002)
                     /gene="fabZ"
                     /locus_tag="IWK70_001434"
     CDS             complement(18583..19002)
                     /gene="fabZ"
                     /locus_tag="IWK70_001434"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9613968.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19027..19503)
                     /gene="accB"
                     /locus_tag="IWK70_001435"
     CDS             complement(19027..19503)
                     /gene="accB"
                     /locus_tag="IWK70_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9613969.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19506..20741)
                     /gene="fabF"
                     /locus_tag="IWK70_001436"
     CDS             complement(19506..20741)
                     /gene="fabF"
                     /locus_tag="IWK70_001436"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9613970.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20760..21497)
                     /gene="fabG"
                     /locus_tag="IWK70_001437"
     CDS             complement(20760..21497)
                     /gene="fabG"
                     /locus_tag="IWK70_001437"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9613971.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21509..22426)
                     /gene="fabD"
                     /locus_tag="IWK70_001438"
     CDS             complement(21509..22426)
                     /gene="fabD"
                     /locus_tag="IWK70_001438"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9613972.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22533..22757)
                     /locus_tag="IWK70_001439"
     CDS             complement(22533..22757)
                     /locus_tag="IWK70_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9613973.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22810..23772)
                     /locus_tag="IWK70_001440"
     CDS             complement(22810..23772)
                     /locus_tag="IWK70_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9613974.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23782..24222)
                     /locus_tag="IWK70_001441"
     CDS             complement(23782..24222)
                     /locus_tag="IWK70_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9613975.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6671 a         5430 c         4243 g         8029 t
ORIGIN      
        1 ttcacttttt cgctaagctt ttcgttttta gccatataga aataaaaaat gatatcgatc
       61 gtagcaaatt gggccaaaag tgaattagtt gccgcacttc gattcgtgat ttcttttggt
      121 ctagatgttt gtacgagtac atcggctatc ttgctaagcg ggttatttcc aaattgcgtc
      181 aacgcaatga tttcgatatt catgcttttc gccagctcgg ctaatgcgac tacatctgca
      241 cttctacctg agtttgaaac aagccaaagc actttctttt tctcattgct gacaagttgc
      301 gggagcaaga cgtggatatc tttttcaaaa atgattggct taccgacacg agaccatttt
      361 tgcagaatat cttctactac taaagaagag gcgccaaccc catataaaaa aacagtctct
      421 gccgattcca aacactctac gactttttgg atcgtttctt cttctagaat atctactgtt
      481 tcttttatcg ctacttgtgc attgttacca agtttgtttt tgatagagta aaaggactca
      541 tttgcttcta tatcactaaa accttcatcg attccagtct gtagttctgt tgaaatcgtg
      601 attttgaagt ccgtcagact ttgaaaaccg atttttttgg aaaaacgtac aactgtcggc
      661 gcactcaacc cggaagcttc tgctaattca ttagctgtca ttgtagggac cttctcaata
      721 ttaccgataa tgtagttggc caactttttt tctgctgaag aataatcttc catcatactt
      781 tcaatacgac taattacgct cataagttcc tccacccttt taatgatagt ctatgtaagt
      841 ataccatatc agacccttat tcacggtcgt atactggata aaaaacaaaa aaacaatccg
      901 cccaacagaa tatcttatcc catcaatccc acagattcta ttgagcatct gtgtctttga
      961 ggaatacctg ttatctattg ttcggattgc tttctatcta ctactttttt acaggaaatt
     1021 tacctattag ttttctctgc tgtaattttt gacttctgct tccatagcat caataaagat
     1081 attcaaatct tctacagctg tttctttact tccataacga cgtacgttca ctgttgcatc
     1141 ttccaattcc ttgtctccaa caactaattg gtaagggatc ttttgtgttt gagaggcacg
     1201 tatcttatag cccatttttt cattacgatc atcaacttct acacgcaacc ccttcatttg
     1261 caaacgttct ttgatttcat aagcataatc gccatgtgca tcaacagaaa cagggatgat
     1321 tgttgcttgt ataggtgcca accatgttgg aaaagcgcct ttgtatactt ctgttaaata
     1381 agcgacaaaa cgttccattg tagaaacaat tccgcggtgg ataactactg gacgatgcgt
     1441 attttccccg tcttctccaa catatgtcaa atcaaagcgc tctggcaata agaagtctaa
     1501 ttgtatagtt gataatgttt cttctgtacc aagagctgtt ttgacttgaa catctagttt
     1561 tggtccgtaa aaggctgcct cgccttctgc ttcaaagtaa tctagttcta attcatccat
     1621 agcggctttc agcattgctt gggctttttc ccacattgcg tcatcatcaa aatatttatc
     1681 tgtattgttt ggatcgcgat agctcaagcg gaaacgataa tctgtaatat tgaagtcagc
     1741 atatactgcc accatcaatt caagcgtacg tttaaattca tctttgattt gatcaggacg
     1801 aacaaatgta tgaccatcat tcaacgtcat ttctcgtaca cgttgtaaac ctgacaaagc
     1861 acctgatttt tcatagcgat gcatcattcc taattcagcg atacgaattg gtaattcacg
     1921 ataagaatgg atcgtgtttt tgtagaccat catgtgatga gggcagttca ttgggcgaag
     1981 aacaagcatt tcgccatctc ccatatccat tggcgggaac atgtcttctt gataatgatc
     2041 ccagtgtcca gatgttttgt acaactcaac atctcccatt attggtgtat atacatgttg
     2101 gtaacctaga ctaacttctt tgtctacgat ataacgttcg atcgtgcgtc ggattgttgc
     2161 cccttttgga agccagaatg gcaaaccaga acctacttct ggggaaacca tgaacaagtc
     2221 aagttctttt cctagtttac gatggtctct ttcttttgct tcttcacgga gacggatata
     2281 ttctttcaaa gctttcttgt caaaaaaggc tgtcccgtat acacgttgca tcatttgatt
     2341 gtttgagttt ccacgccaat aagcacctgc cactgataat aatttaaata cttgtatacg
     2401 gccagtggat ggaacatgtg gtccacgaca caaatcgatg aattcgcctt gttgataagc
     2461 agtaatcact tcttcttctg gcaattcact aatcagttct actttgtacg gatcacttgc
     2521 aaaaatttct aatgcttcct gttttgaaag aacacggcgt tcgatcggca gattttcttt
     2581 gacgattttc atcatttctg cttcgattgc cggcaaatct tctgcagtta cttggttttg
     2641 tccattatct gtatcgtagt aaaaccctga atcgatagct ggtccaacgc caaagtggat
     2701 atttggatag aggcgacgca tggcttgggc catcaaatga gctgctgaat gacggactaa
     2761 gccaagtgca tcttcatggt ctggtgtgat gatctcaata cttgcatctt cctcgattgg
     2821 acgagtcaaa tcaacgagtt ttccgttcac tttaccagct aaagcttttt ttgctaagct
     2881 ttttgaaatg ctttcagcaa ttgccaatgt tgttgttcca gactcaaatt ctttgactgc
     2941 accatctgga aaagtaattt ttatcataat agtttcctcc ttgaaatgtt ttgattgatc
     3001 agatcatcaa gctgaaaaac aaaaaaatcc tagcatctct tgaaaagaga tactaggacg
     3061 aaaagttcgt ggttccacct aatgttcaaa cgcaaatgcg ttcctcaagc gttgtaacgg
     3121 tccgaccgcc cttgcttcaa caagggtttc gaaagtggtc atcctttggt tttctcctgg
     3181 aaaagtttca gcctagcttt tcctctctta tcgaagactc tcaaattcag ttggctttct
     3241 catcaatcaa ttatgtgttt atttaaccac taataaatag aaaaatcaag tgggaaacta
     3301 aacttatttt ttccattccc catttacacc gtatgttcct tttttcatct cattgatgat
     3361 gacatggatg tgttcttttg gtgcctgcgt gtttttatga acagcttccg tgatatcttt
     3421 catcatattt tctaattgtt ccggactgcg accttctact aattcgacat gtacgaatgg
     3481 cataatgctc tctcctcttt ctgatcgttc ttttttcaac tcgattctat catactatgt
     3541 ttgtcttggg tactgaatca ggaaaaatgg ctggatcttt ttttgctctc aaggcactca
     3601 aaatagagac cgtatccacg acttcttgca aaacagctcc gaataatgcg gggattagtc
     3661 ccgtacttgc aattaacatc aaaaaaatac aaataaagat gccaatcagt acagactgtt
     3721 tcgctattct catcgtatct cttgaaatca ctactgcttt tgctacacgc aagagatcat
     3781 ctttcaagat gaccgcatct gcactttcac tagcagctgt agaaccatga gcccccattg
     3841 caataccgat atctgcagca gcaagtgctg gtgcgtcatt cacgccgtct cctaccatcg
     3901 caacaggtct ttgatcttta gggatacttt ctaaaatggt cagtttatct tgaggcaagc
     3961 tacgggcatg tacagaatct aattggactt tttctgcaat cgtttgagca atggcagatc
     4021 catccccggt gatcatcatc aaatttttca catcaagttt tcttagctcc ttgattgtct
     4081 ttgctgcttc tgggcgaatc tgatccgaaa ataggatcgt tcctgcaaaa gatcgatcaa
     4141 ttgttacata gattgctgta ttggacgttt ctatttcttc agctgaaaca aaagaggcac
     4201 gtccaatctt tacttcatga ttattgacta ctgcttggat tccttgaccg gtcacttctt
     4261 tcagttgact aacaggatac agttggctgt ttttcacgtt gttcactaac gaacgagcta
     4321 agatatggct agattcctgt tctgcactag ctgcatacag aagtagctct tcttttttta
     4381 ttccattgac tggctgaatc gcctctactt ccaatatccc tttggtaagc gttcctgttt
     4441 tatcaaaagc aatcgttttt gtacgtgcga gcttttcaat cgttgttcca gtttttacaa
     4501 cgatcccgtt tttacttgaa cggctcattc ctgcaactaa agcaatgggc gcagctagaa
     4561 tcagtggaca aggtgatgcc actaccagta cctcagcaaa tcgtaaaggg tcttttgaga
     4621 accaccaaga aataccaccg atcaaataag caatcaacgt gaaagggact gcataacgat
     4681 cagctaaacg gacaaatcgg gcaggctttt cttttgattc ttcgactagt ttaataatca
     4741 tttgatactg gctgtctgcg gcacgttttt cagcgatcat ttggatcgat ccgtctccat
     4801 ttaaagaccc tgacataatc tgatctccag gtttcttggt ttccgggcgg gactcacctg
     4861 tcaaagaaga ttcatcgacc gaagattctc ctttggtcac tcgaccatct gcgggtacaa
     4921 gttcgccagg cttgactagt aactggtcat caatatttat ttcatcgatt actatatcct
     4981 gcaattttcc ttctttgaat cgatgggcaa ttcttggggt gttatctaac aaagactgca
     5041 gttctttacc cgctcgttta ttcgcgtaat cctctagact atctccgccc gtaagcatga
     5101 tcaatatcat caaactagcc cagtattctt tgacggctaa tgttgcaagg attgcggtaa
     5161 tcgctaatac atctacgcca tatcttcctg aacgtaacgt cttgatcatt tccaaaaaca
     5221 tcaagaaggc gagaatccct ccagtaaatg cgacaatcgc aaatgcaagg gccggctggt
     5281 gaaaaacaaa ttcacttaat aaagctatcc caccaataag caaagtaagt aaaaattttt
     5341 tgaaatgtgt cattgttttt tctcctcttt tatgtgactt agagaggtta gtaataaatt
     5401 tccagctgcc tctttcatct taagcatagt ccttgactag cactttttaa aacaataggc
     5461 cttaattgaa aacaactatc actatcagcg tttccaaaat tcatcagttc tacaaacaaa
     5521 aatattcagc aaggattact ttttcgattc atcagttaaa aagtaccttt tttcgaaaaa
     5581 ctatgctata cttttaattc gttatttacg gcggaaggag caaacatgcg atgaatgaga
     5641 aacaactaga acaaacgcac gtggatgaaa cgatcgaact aatccagatc gaacaaaata
     5701 tgctggatac aaaacaacaa gaattgactc aggaaatgaa tcattcgaca aaagacacag
     5761 ctaatcacaa gattcgagct ggttccaatg aagcttttta tgaatctgct gttgaatatc
     5821 gccaacatga gcaggaattg cttttgaaat accatactct tgaagcacag caaaaacgcc
     5881 tgcaaacgtt gtctgtcatg aaagatagtc cttattttgc ccgtatcgac tttaaagaag
     5941 accgtgaaac agaaacatta tatctaggta tcgcttcttt aagagatctg gcagaagaaa
     6001 caatcgttat tgactggcgc gcaccaattg ccaatcttta ttatgaaggt gagattggtc
     6061 ccgcatttta tgaaactgaa acagatagaa tagatgtcga tctgcagttg aaacgacaat
     6121 ttaaaatcca agaaggacgt attttatcta tggtagatac gtcggaagta atcaatgatg
     6181 actttttgct agagatattg gatgaatcat caagcaacca tatgaaaaac atcgtctcta
     6241 cgatacaaaa agcacaaaat gcaatcattc gggacacaac aagtaaagta atgctgattg
     6301 aaggaatcgc cggaagtgga aagacatccg ctttgcttca acgtattgct tttctgttgt
     6361 atcataatcg caagtggctc gaggctgaaa acgttcttct attttcaccc aaccatttgt
     6421 tttctgatta catctcgact gtattgcctt cattaggtga aagcggcgtg cctacacaga
     6481 cattcaaaaa ttatttggtg cagctgctgc cagagttttc tttactagat gaacagcaac
     6541 aagaaagtgg ttttttgcta ggggaaaaag atccgataca agtgatgaaa agcggattga
     6601 ccttagtcga tcagattgat cgctatatcc aaggcatcac aaactacggt cccttgttcc
     6661 gtgatatgaa aataaatggc cgtacgatcc tatcaaagga atcgattcgc caatggtata
     6721 aagaaacaaa tgaacaatta cctttacatc atagactttc tctattacaa acgaagctcc
     6781 tgaaaaaatt aggcggtctg caaaaagacg aaacacgtca gcaatgggtc aaagatctgg
     6841 cggaagaaca gctccaagaa ctatacgcaa cagatccaaa tcttgaatat acagaacaaa
     6901 aggagcgttc attacgtaaa aaactggcca accaaatcgt acgtaaaaga ttccgtcgaa
     6961 tccatcgagg gatcatgaat tatcaatttg tcaatataac gaaacaatat ctccactttc
     7021 tacaaactgt gccactttct cttctaacac gacataaaat cagcgagacc aggtggcagg
     7081 aacatttgac aactgttcgt acacacttgc gtcaaagaga attgcgtcaa gaggatgctg
     7141 tactattctt tttactgatg cgccgaacat atccagtgac ggttacacac aaagctcgct
     7201 atatctttat tgatgaaatg caggatttcg caccatcaca agtggcattg ctgcaagcct
     7261 tgttcccaaa agcaaatttg actttctgtg gagatctaaa tcaaaatgtc tttggaaatg
     7321 aaacgatcgt tggatcattg gaagaccttt tcgtggaccg tgaagtcact cgtttccaat
     7381 taacgacaag ctatcgctcc acaaaagaaa tcactgattt tgctaatcat tttattgtga
     7441 aagaacatca agtagaaaca acagcaagaa aaggagacct tcctttgctc atccaaggaa
     7501 caacagatga tatcaaattg aattggttag cagataccat tcgaacgact cagggaaaag
     7561 cccggtattg gcgtacagca atcatagcta aaacacaaga agaatgcgaa gctctttatg
     7621 ctcaactgcc agacgagttg aaacaacaag tccagctgat tttaaatgag agtgacttta
     7681 tgaagcgttc cattatttta cttccagcct ttttagcaaa aggtctggag tttgaccgag
     7741 ttttcttgtg ggatgtagat gcagaacatt tttcttctca gcaagacaaa ttgatccttt
     7801 acaccatgtg tacacgtgcg atgcatgaat tgaccctttt tacctccaat aatgaaagtc
     7861 ctttgttaaa caatatggat atttcatctt atgagaaaaa agtactgtaa gtaaaaagac
     7921 aactgctatc attaatgtta gcagttgtct ttttatttac aataataatt gcagaaaaag
     7981 taattcaatc atttaaggtc atttattcta tatataaatc caaatctgtt tcaggatatt
     8041 ttaatttgac aagcgaaata atcattttca gttcttccat gttttctgga tattctgggt
     8101 caaatacctt gctatgacgg ccaatcccgc cattgtcgat caatcggtaa attcgattcc
     8161 attcaatgaa ttggtcaatt gattggaaat tgctgattgg atagccgcca caatccaaaa
     8221 tctctgatac acgaaatgct aggtatgcac tatcgatcac tttatttttt ttcgtgtcta
     8281 tcttctcatt tctttcgata agaatttttt caggacgatg tttcaagctc cactctaata
     8341 gtagaaagaa agcctccatc cgatactctt cttcaaataa ccgaaaggct gaatcagaac
     8401 gacgaaaaag cagcggaatc agataatttt gtctataaat cagcattggt gcgcggtcca
     8461 ccatacactc taaataacgg ttcagatacc ctgtaccgac atagtttcct aatcgatagc
     8521 cttcaacgcc gaatcttgcg tatttttgcg ctaattttga acaatcagtt acgttcacag
     8581 ctaactcttc tactttgcgg taaccgctta agatacgttc aaaaggttct aaagtgattt
     8641 ttgcttccat attcttcccc gtccatcttc cacatacttc atatcatttc acatgatact
     8701 tatgctctca tcataccaca ggttgaatta gaaaagaaaa taaatcacac aaaaaagcaa
     8761 gacaatctgt tagtgagatt gtcttgcttt tcgaaaaaga ctgaatactt ttcgttaaaa
     8821 ctggttgctt agtcttctaa tgccacatta tggaacacat gctgtacatc ttctagatct
     8881 tccagtgcat caatcatttt ttcaaatgtt tcttgatctt ctggagatag tgttacctcg
     8941 ttttgaggga tcatttggat ctcagcgatt gaaaaatcag tgattccttt ttcttttaat
     9001 gcttcttgta tactatggaa atccgctact tcaccgtaaa cgatgatttg tccttcttct
     9061 tccattacat cgcgaacatc gatgtctttt tccattaggt attcaaggat ctcgtcagcg
     9121 tcttctcctg caaaagcaaa gattgctgta ttgtcaaaca tgtaagaaac cgctccggaa
     9181 acacccatgt tcccgccatt tttcccaaat gctgcccgta cgtctgctgc tgttcgattc
     9241 acattgttcg tcaatgtatc aacaatcacc attgagccgc ttggtccaaa tccttcatag
     9301 cgcaattcag agtatgtttc atctcctgca ccttttgctt tttcgattgc tcggtcaata
     9361 atatgttttg gtacattata agttttagcg cgatcaatga cgaaacgtag tttttggttt
     9421 gcatgcggat caggatcacc tgattttgct gccgcgtaga tttcaatccc aaattttgca
     9481 taaatccggc tattattagc atctttagca gttttctttg ctacgatatt tgcccattta
     9541 cgtcccatat tttcacttcg ctttctgatt tgatagtttc ataacgcaaa aacgtcctgt
     9601 tccattatac ttgtgaatca cgattctgtg aagctttttt tctttcttcc ttcattcttc
     9661 tcatttttag tcaattatct atactcggat tgggatttgc ccgaaattct gttccggaat
     9721 attgcctccc aattgataga tttgatcact tctaactaac agttccgctt cttgtgcctc
     9781 tttttttccg attcgggaat aaatagaaat ctcttttttt tcttttaaga atcttcttcc
     9841 agcatcggta aatccaagta gtcgaagata atttttattt tgttggacag gaatctcttg
     9901 ctttttgata ttcagcaaga cataacaaga taatcgttgg atccgtgtcc atgtatatct
     9961 ttttgttttt acctttgaca caaaatcctc aaaactatca gcagttgcag ctgctgtttt
    10021 catgcgcgat tctattcctt cggtcatctg atagatatct gtcagttcca gcaaagtaga
    10081 agataacaac cgataacgca aatacggcca ataagattcc caagtctgca gcgaacaagt
    10141 tgaaagatct tcttctgtct gaggcggcac aagtccagct attctttttc cttcttctaa
    10201 cgcatggcga atggctgttg cactggctat cctctgatct gtgatgattt tttcatgata
    10261 gcctgcactt ttacgtcgga tcggatgtaa ttccataggg tggacatact tggcattttc
    10321 ctttgcataa cttaatccca agatatgatt tggcgaagaa aaatcaagcg taatatcggg
    10381 atacagactg cgaaaaacct cagtcatttt ttgcggataa ctatacgttt gatcagacag
    10441 ttcttgaaat gcctcttcta tcctatcttg attctcttgg acaaagcgtc cgaaactgct
    10501 gtaatcaaac gaagtctctt cttctgttcc aaaacacaaa ctgtcacagt ccagtgtttg
    10561 caagattttt actgcacctt tagcaaaata gtcagctgac tgaaccgccc attcgacggg
    10621 aagctccact actaaatcca caccattttg taaagcttca ttcgcgcgat gccatttatc
    10681 gatgatagcc ggttcgcctc tttgcaaaaa attcccgctc attacagcaa tgacgacctc
    10741 tgctcctgtt atttttcgtg ccatttccac atgatagcga tgtccattgt gaaaaggatt
    10801 gtactctacg ataatcccgc agcttttcat gcgcatcctc cttcatagtt atcgataaaa
    10861 aagaaataga gacaagtaaa aaaaggcaaa aatcggaaaa aatacccgaa ctatataaac
    10921 tattctcgca aacacaatac ttctgcgacg aaaagctgca ctgcaagagc aatgcctata
    10981 tcgttcggat atataattta tgttgaagat ttattctttt cttgttaact cgagcaggca
    11041 aaaccgctag tctttgtatg ctacaaagaa ccagcgcttt gatttctctg atgctgcttc
    11101 gtctgtaaaa tcagaaaatg cttcgacttt cgaaaagccg ctgttttcca gcattcttaa
    11161 ataactttcc aatggatagg tacgctcttg atgtagttca tcttcacgaa taaatcgacc
    11221 agacccttct tgttcttcaa cgaaaaaggt aagaaaatgt tcgatgctgt gtttctcgct
    11281 tcctggataa ctatcccaca aaaaagcaaa attatcccct tgataatgat agctatattc
    11341 tggaaaaacc tcatctactt gataggtgga gtgtacgtca aataaaaatc ggccattttc
    11401 ttctaacgct tgatagactt catcaaacac ttgctgtacc tcttgtctat ttttcatata
    11461 gcacagcgaa tcagaaaaac aggtaattgc ctgatattgt ccgatttctg ataaatcgag
    11521 catattgcct tccacaaatt ggatcgatgc gtcttcttca tatgctcgct ggctggcaat
    11581 catcaacatc tcttctgaaa ggtctaaccc tgtcacttta tatccatctt ttgcaaaggc
    11641 aaccgctaat gctccagtac cgcatgccag ttccaaaata ttttttgtgt cttcttgtaa
    11701 atgacgtttc gaaaaagcta accattgctg ataaagtgtt tcgtccatca cttcatcgta
    11761 aatgaaagca aatgtttcat aagccatatg ttaatccaac cattcactaa gatcgactag
    11821 cggagcatct gaccatagtt tttccagatt atagaaattt cgttctggcg catggaaaac
    11881 atgtacgatc acatctccaa gatcgatcaa tacccatttt ccgccttctt ttccttcaat
    11941 acgttttact tcatatttgt tttcttcttc tttatcaatg atttcttctg taattgcgtt
    12001 gatttgacgt tcgctatttg ctgaacagat catgaaataa tctgccaaca aggaaacatt
    12061 tcgaacgtct aaagcgataa tgtcttctgc ccgttttgaa tctgctgctt caatcgcaat
    12121 ttttaattgt tctttactat ctatgatgat ttcctcctaa tatgagcagc ttattttttt
    12181 gctacccagt aattatatgt ttcgattgtt tttggataaa tctgttgttc ttgcgcaagc
    12241 aaatgggcca atgtatgttt tgtttcaaat gctacagctt cgtctagatc agcccatgca
    12301 atggctcgtg catcttgtac cccaggaaaa tctcgacctg gttcaatata gtctgctaca
    12361 taaatgattt tgtcaaggag actcatctta gcagccccag tcgtatgcac ccggattgca
    12421 tgaagaatct ctgcatcggt gattcctaat tcccgctcta caaacgaagc gccaacaaga
    12481 ccatgccaga ttgcattatt ataattcaat aactccggat caaaaccgtc tcttacgatt
    12541 accattttga actcctcatc tggtcgttct tttgcataat catgtgtcaa tgcagcaata
    12601 ctagcttttt caggagaagc accgtatttt ttggcaagag ccacggctgt ttcttctact
    12661 ccaagcacat gcttgaagcg ttgttcgctc attctcatct gtattttttg catcaggttt
    12721 tctctagaat aagcagtata ttcattacta tagtccatat tcatacaacc cttcattttg
    12781 aatataatca attactttat ctggaacaag gtagcggata gaacagcctt ctttgatctt
    12841 ttgacgtatt ttcgtggaac tgatgtcgat ctcaggtaca tctacccaaa ttacaggata
    12901 tggagtatcc gttgtgtatc ctggtcgacg tatacccaca aagttgacca tagaagtcag
    12961 ctcatcgatt ttataccatt ttggcaaata ttctaccata tccccaccga taataaagta
    13021 ataatctgta tccggattgt tttgcgtcaa ttccttcata gtatcatagg tgtaactttt
    13081 cccaccacga gctaattcaa tcgtttcaat ctgcaaaaaa ggattgtctt ccaccgctaa
    13141 ttccaacatg ttgagtcgat gttttgcatc aatcgtctgt ttctcatcaa catgaggtgg
    13201 ctgataagaa ggcatcaaaa atacgcggtc aagacctaaa ttccggccag cttgttcagc
    13261 catcactaaa tgtgcaaggt gaacgggatt gaatgttccg cccaagatcc ctacttgctt
    13321 tttcttctct aagaattgag gcatttcttc aggaaataat tgtgaacgaa caaatgtctt
    13381 agcttgcgta ttcatcatgg tcactcctta tattgctttt acttcttttg atagcctttg
    13441 atacttttct tttgtggaag gtttgtataa tacaataaca cgtccaatga tttggacgat
    13501 ttcgcaatct atttcttttt ctaatacttg agctgcttct tctgcaatct catctgtatt
    13561 ttgaagtaac gatactttga tcagttctct tttttctaaa gcttcttcaa tttgaacgat
    13621 cattgctgaa ttgatcccac cttttccgat ttgaaaaatc ggctgaagat gatgcgcttt
    13681 gctacgtaaa aaacgctttt gttttcctct taaactcata attttccttc ttccttaaat
    13741 caatgctttg cgtttcaata catctacacc ctttggcgcc cagcccgcaa ttactactgg
    13801 cgctgttaca gtgatccagc ctagaccggc aaaaacgatg tctgtttttt ctttcacaga
    13861 aaactcaaaa cgaacaagct ccggaaatgc ctcgacttca tcaggacgag gaggctgtaa
    13921 taatccacct acatgttttt gataaaattc gtctgctttt tccagtttcg ttcgatgaat
    13981 atttacatca tttgacacat aagcaataaa tgaagcacga ttgccgctga taaaatcaaa
    14041 tcttgcaagt ccgcccaaga aaagtgtttg cccttcattc agctgataaa ctttaggttt
    14101 gatttctttt aacggtgcaa tgatccgcaa atctttttta cctaagtagt gcgccatttg
    14161 atggcgatgg ataattcccg gtgtatcaat caaaaaatgc ccgtcatcta aaggaatctc
    14221 gattttatcc aaggtcgttc ctggaaactg cgaagttgtg attacatctt gtactcccgc
    14281 tgtatttttg atgatttgat tgataagcgt cgattttccg acatttgtta ctccgacgat
    14341 atagacatcc cgtccatcgc ggtattcttc gattttttct aatagttccg tcatctcatg
    14401 agatttctta gcactagtta ataatacatc tactggacga attcctgctt cgtaagcacg
    14461 ttccttcatc cattgtgtta atttagtttt tttcaatgat ttaggtaata tatccacttt
    14521 gttcccaact aaaagtactg gattgtcacc gacaaaacgg tgtagtcctg ggattagtga
    14581 accattgaag tcaaaaatat ccaccacatt gacgatcaat gcatcttttt gccctaattc
    14641 attcagtaag cgcaaaaagt cgtcatccgt caatgagaca tcttgggtct cattataatg
    14701 gcgtaatctg aaacaacgct gacaatagac ttccccggaa gctaaacctt tttccaaagc
    14761 cgtttgcggc gtgtatccta attcaccggg tttgtttgtt tgtataacgg caccacagcc
    14821 gatacattgt aattcttctg tcattcgatt ccacctcgcc agcccatatc agggtgtttt
    14881 gctaatagat aggccataat tcttttttcc caaaaacgat taaattgtgt cttccaacta
    14941 tctgttgcga taatgggttt tacaaggatg ctacgtatcc ctgcacgatt ggctccacgt
    15001 atatcagtca taatctgatc gcctaccatt actatctcat catctgctaa atccaattgt
    15061 ttctttgcta tgttgatccc tcttgctaaa ggtttcattg ctcgtgcaac atacaaaaga
    15121 tcaaattttt caatcgctcg cgcgacacgt tttgaattat tgtttgaaac aacaacaaca
    15181 gggattcctg catttttcat ttctaaaatc caagtaagca actcttctgt tccatctgga
    15241 ttattccatg caatcaacgt attgtctaaa tcggtcaata ctgctttgat gcctagattc
    15301 tttaattggg caggtgtaat ctgatagatg gcttctacca tccatgttgg tttgtaattt
    15361 gaaaacatgc tgtctcctca tctgcaaaaa atgaaggagc acataaagta ctcctcaacc
    15421 ccgtattata ctgcatatca gcaaaaaaaa ctacctctat actcgttcgt ctttcagaaa
    15481 aagcatttct ttacaaaatg accaaaaata aagattgatc acagaaaaat ttagaaacaa
    15541 aaaacaacta atattggcgg aacctcttgt atcgtttttc cagtaattta tgctgatcta
    15601 atcgaacaag ttccgtcaat ctttcaatca acgctttttt cataatgtgt gcgactttat
    15661 atcgtggcaa ctgctcgccg ttgaacgttt caggaataac tcgatcaacg attcctaact
    15721 cgcgtagttc tggcgcagta attttcatca actcagctgc ttctgcggcg cgactaccgt
    15781 ctttccacaa aatcgacgca aatccttctg gagaaagcac agcataaatc gtttcttcca
    15841 gcatccagac ttcatccgct aaacctagag caagggcacc gccacttcca ccttcaccaa
    15901 tgatgatggc gatgatcggt acctttaaat cagacatttc gagcagattt ctggcaatag
    15961 cctctccttc gccgcgctct tctgctccta caccgcaata tgcacctgct gtattgatca
    16021 aggtaatcac cggtctattg aacttttccg cttgtttcat cagtcgtaaa gcttttcgat
    16081 agccttcagg atggggagaa ccaaaatttc tgcggatatt ttcctgcaga tcccgtcctt
    16141 tttgaatacc gatcactgtt accggtttgc catctaatcg agcgattcca ccaacaatcg
    16201 cttcgtcatc tgcaaataaa cgatcaccat ggcattcctc gaaagaatcg aagatctctt
    16261 caaaaaaatc caagcttgtc catcgttctt tagctcttgc taactgaacg acatcatgtg
    16321 ctgtcttatc cacgatctat ccatcctttc tgtgtatgca attcaagaag cgtatggatt
    16381 ttctgtttca attccatgcg gggaacaatc tgatcgacaa aaccatgaga taataagaat
    16441 tcagcctttt ggaaatcttc tggaagttcc tgcttgatcg tctgttcgat gacgcgtctt
    16501 ccagcaaaac cgatcaagct ttggggttct gctagaataa tatcgccttc cattgcaaaa
    16561 ctggcagtta ctcctccggt cgtcgggtcc gttaaaacag ttagataaaa aagaccttca
    16621 ttgctatgcc gtttgacagc agcagagatt tttgccatct gcattagaga aaagattcct
    16681 tcttgcatcc gtgcgccacc tgaagctgtg aacaagacga ctggaagact ttcctttgtt
    16741 gctcgttcga ataatctggt gatcttttct ccgacaatcg ttcccatact tcccatgata
    16801 aaattaggat ccatcactcc aataatgaag ggaatcccct gaatggttgc tttaccagtg
    16861 aggactgctt catgaagtcc tgttttatct tgcatttttt taattttttc cgtatattcg
    16921 ggaaatgcta gcggatcttt ggttacaaga tccgtatccc attcttcaaa acttttttca
    16981 tcaataatca aagctagacg ctgccatgca ccgattcgaa aagcgtatcc gcaatgaggg
    17041 catacttttt cttcaccgat gtctttcgta taaaggatat gtttgcaatt tggacacttt
    17101 gcccacatgt tatcaggaac agatggcgag ttagcaggtt tgctctctct attaggattg
    17161 atccgaatat atttcttttt cttaaacaga gccatctatg cgcctcctat ctccgtttct
    17221 ggtgtccaat tcggtaaaaa ttcttcttgt aaaaatgctg tgctgtaatc acctgctatg
    17281 actgcaggat gactaatcaa atccatctga aattctgcgt tcgtgatgat tccttctgtg
    17341 ataaattcac ttaaggctcg ctgcatcttc attaatgctt caaaacgcgt atttccatgg
    17401 acgattattt ttgcaatcat tgaatcataa tatggcggga tcgtatatcc cgggtaaact
    17461 gcactgtcta ctcgcagtcc catcccacct gctggaagca gtaagttttg gatcttccct
    17521 ggagaaggag caaaatggaa agcaggattt tctgcattaa tccggcattc gattgcgtgt
    17581 ccttggaata ctacttcttc ttgacacaca ttcaaaggct caccggctgc tatctccacc
    17641 tgttttttta ctaaatcgat tcctgtgacc atttctgtta ctggatgttc cacttggatt
    17701 cgagtattca tttccatgaa ataaaaatcg ccttcttcat ccatcagaaa ctcgatcgtt
    17761 ccggcatttt cataattgac tgctttagct gctcgaacag cagcgtctcc tagtgcttct
    17821 ctttttgttt gtgagatcac tacagaagga gattcttcca gtactttttg attgttgcgt
    17881 tgtaaagaac aatctcgctc gcctaaatga atgacatgac caaatcgatc acctaagatc
    17941 tgaacttcta tatggcgagc aggataaatg attttttcca aatacatatc atcatttcca
    18001 aatgccgctg ctgcttcttg ctgagctgaa gaaaaatgct gtgggagctc ttcttttgat
    18061 tgaactttcc gaatgccttt cccgccacca cctgcagctg ctttcagcat cactggataa
    18121 ccaattctgt ctgctatttc caatgcttct tctactgtag ataatacacc agtacttcct
    18181 ggaatgactg ggacatttgc ttcttgcatc aattgacgcg cattgatctt atttcccatt
    18241 gcatcgatcg ttgcactttt aggaccaata aatgtgatat tgcactcttc acacatggag
    18301 gcaaatcggc tattttcaga taaaaaacca aatcctggat gaattgcttc ggcttttgtc
    18361 acgattgcag cgctcagtac ttcctgaaca ttcagatatg aatcaacggc ttttgccggg
    18421 ccaatgcaga ttgcttcatc tgctaactgt gtatgaagtg cttcggcatc tgcttctgaa
    18481 tatatcgcta cggtttggat ccctagttcg cggcatgcac gaatgatacg tacagcgatt
    18541 tcaccgcggt tagctatcaa tactttagaa aacatatttc acctatccaa tcataaaggt
    18601 taattccgct tcggctactt ttttaccgtc tactttagcg attcctttgc ctattccggc
    18661 tgaagctttc actttcaata tctcaacttc aaggtataag gtatctcccg gtgtaacttt
    18721 cttacggaat tttgctttgt ccaatccgcc gaaataagcc gtctttccct tgaattgttc
    18781 aagagaaagt aatgcaactg ctcctgcttg agccattgct tcaacaatca atacccctgg
    18841 cataacaggt tcttctggaa aatgtccttg aaaaaagggc tcattgatcg ttacattttt
    18901 cttagcaacg atccgttctc cttcaaccat ttcttccact cgatcaatca aaagcattgg
    18961 ataacgatgt ggaatgattt cttttatttc ttgtatgttc attgttttcc ctctttcctg
    19021 ctttgcttat ttttgctgaa tcctaaagag tggctgtcca tattcgacaa ctgactcgtt
    19081 ttctacaagg atttctgtga tcactccgtc ggcctcacta gttatctcat tcatcagttt
    19141 catcgcttca ataatgcaga taacttctcc tttggcaact tgatcaccta cttttttgaa
    19201 agctggtttg tcgggcgctg gttgcagata aacgattccc acgattggcg aaacaacctc
    19261 gattccatca aatactgccg attctttttc tttcattatt tctggagcag ttgttgcttg
    19321 ttcaggtatg acattgactg gctccgaaac tggcaacgtt tcgtttgttt gaaccatatt
    19381 catgcctcga cttgttttat ttttattcat atacaattca aattgacctt cacgcaaatc
    19441 gaattccgtt agcgaggatt gatcaaattg actgactaat tctctgattt cattgatatc
    19501 caaaattatt cctcccagcg tttcatgcac agaaccgcat tatgtccgcc aaaccctaat
    19561 gaattactca ttgcataagt aaaacttgca ggtttgcttt cgtttttgac aactgtcaag
    19621 tcaatctcag gatcttgttt ttctacattg atcgtaggtg ggataaattg atgttctaat
    19681 gccagcaaag ttgccaaggc ttcgatcccg ccggctgctc ccaataaatg tccagtcatc
    19741 gatttcgtgc tgctgacgta agtatctctg aaattttcac ctaatgcgat ttggattgct
    19801 tttgcttctg cctggtcatt ggctggtgta cttgttccgt gagcattgat atatccgact
    19861 tgttccggct gtacaccagc ttcttccatc gcaagcttca ttgctttaga tgcaccagaa
    19921 ccatctggag taggagctgt catatggtaa gcgtcacagt ttgcaccgta tccaacgatt
    19981 tctccataga ttttggcacc gcgagcttgt gcatgttcta aagattcaag aactagaacg
    20041 cctgctcctt cacccatcac aaaaccattc cgattttcgt caaacggaat cgaaccacga
    20101 ttaggatcag ttgattctga gagtgctgtt aaagctgcaa atccagcaat ccccatctca
    20161 caaatcgttg cttctgttcc tccagcaaga atgacatcag aatagccatg tttgatgtta
    20221 cggaatgctt ccccaattga attcgttccg ctagcacaag ctgttacgat agctgtacaa
    20281 ataccttttg cacctacacg caaagcaata tttccagctg ccatgttggc aatcgccatt
    20341 ggtacgaaca ttggagaaac tcgttttgca cctttttcat gtagacgaat aacttggttt
    20401 tcgattgttg gaagaccacc gatcccagat ccgatgatga cgccaaaccg gtcttgattc
    20461 attttttctt tatctaaacc gctcatctcc attgcttcta aagcagcgta gattccatag
    20521 atcgaaaaca tatccattcg tttgctatct tttttaataa aatatttatc gtaagggaaa
    20581 tctttgactt cacccgctaa ggtaatccct gtttctgaag catcaaattt agtgatcggt
    20641 gcgatgccat tcgtcccctc ttttaagcta ttcaaaaagc tgtctgcgtc atttccaata
    20701 ggtgaagtga ctccatagcc agtaattacc actcgattca tttgtttgct ctccttttct
    20761 tatccgtgca tgaccaatcc gccatcgaca ttgatgactt gtccggtgat atatggattt
    20821 tgtgctaaga aaactgctgt ttgtgcaata tcttctactt gtccgaaacg ctgcatcgga
    20881 atttgctttt ccgcttgttc cttgactttg tctgctaaaa cttccgtcat atcagttgtg
    20941 atgaatcctg gcgcaatggc attacatgta atgcctctag ttgcagcttc tcgtgccaca
    21001 gattttgtta atcctactac gccagcttta ctagctgcat aatttgcttg accaatattt
    21061 ccaatcaacc cagatacact agagagatta atgatggccc cttctcgctg cttcatcatt
    21121 tttttcaata cgtgctgcgt catattgaag gttccaatca aattgatatc cagacatttt
    21181 ttgaaatctt ctgcatccat tcgcatcaca agtttgtcat ttgtgattcc ggcattgttt
    21241 actaacacat ggatcgatcc aagcttttct tctgcttctt tgatcatttg tcccgctttc
    21301 tcataatcag agatgtcacc ggaaactcct acgcatttta caccgaatgc ttcgatttct
    21361 tctatctttt cttttggaat ctctccgcga ccattcaaaa tgatatttgc tccagctttt
    21421 gcaaaagcta aggccatcgc tttgccgatt cctcttgtac tacctgtgat aaatacattt
    21481 tttcctttta catccataac gttcctcctt atagtattgc tattgtttca tcaaatgtct
    21541 gcttattctc aacacgcaga actggtattg ttttatcgat ttttttcata aatccgctta
    21601 atacttttcc ggggccgact tcgatgactt gttcgatgcc gattgttttt aacttatgga
    21661 tactctcata gaaacgaaca ggttgcatga cttgctgttc aagtagtccc gcgattgttt
    21721 ctttcttcat tatttccgtt gttgtgttgc ttatgatagg aaaagaaggt tcagagaatt
    21781 gaatctgact taggtccttc gccagttttt ttgctgccgg ttctaaaata gctgtatgaa
    21841 aaggcccgct gacatttaga gggatcattc ttttgaaccc tttttctttt aatagtaaaa
    21901 cagcttcgtc tactgctttt tcttcgccac caatcacgat ctgctgaggt gtattataat
    21961 ttgcaggaga tacgatgccg tacttgctag cttcatgaca gctttcctcg atcgtttcaa
    22021 tcggcgcatt catcactgca accattttcc cgcttccagc tggggcagct tctgtcatat
    22081 aagcgcctct ttttgctact aacgcaactg cttcagtgaa ggatagcgca ttactagcta
    22141 ctaatgccga atattctcct aaactcagtc ctaaagctgc atctgggatg attccatgct
    22201 ccttcaataa gcgataatac gcgatgctga ctgttagaat agctggctgt gtatattgcg
    22261 tctgatccaa acgttcattt tctgtaaagc aaagttcagc catatcgtag ccgagaatct
    22321 cacttgcttc atcaaatgtc tgctttacaa cggcttcttc atataaatct tttcccatac
    22381 cttgatattg tgcaccttgg ccactaaata agaatgctgt tttcatgttt ttctccaata
    22441 tatccagaat atatgtttga ttcttcctat tcaaaaaggc cgagacattc ctatttcagg
    22501 aaatgtccca gcccccaacc taattttaat gattatgcag tttgtttttc tacgtaagtc
    22561 actaaatctt gaaccgtttt gattccgtct tctgtttcga ttttcacgtc aaattcatct
    22621 tcaatttcat tgatgatttg gaataaatcc aagctgtcag cttctaagtc ttcttggatg
    22681 tcagtagtca atttgatttc ttcagtttct tttcctaatt cttctgcgat gatttcttga
    22741 attttttcga ataccataat aatatattcc tccgatattg tttgcttttt atttttataa
    22801 tttaacagtc tacaactcca tgagaatact tccccatgtt aaacctccgc catagcctgt
    22861 aaatattact ttctgctgtt ttcctaaggt gatctttcca ctagaaactg cttcatccaa
    22921 taagataggg atgcttgcag cagatgtatt cccatattta tccatattgg tcaggaactt
    22981 ttcttgcggc atctttactt ttcgggcaat tttctcaata attctgaggt ttgcctgatg
    23041 caaaagcaga taatcaactg gtttgtcttt catgacttct tggatattgt ttgcaacgtc
    23101 tcgtacggca aaatcaaaaa tatctctgcc agtcatttgc aaataatacg aaccttctga
    23161 atcttctgaa tagaacggat ttttattttg ctggtagcca gcggtcaatg agctacttct
    23221 ggtaccgtca gctgctaatt tttctttcag caattttttc tgtggattcg cttctaagag
    23281 aatcccacct gctccatcgc caaacaacac agcagtcgta cgatcagacc aatcgattag
    23341 cttagatagt gtttcaccac caatcaccag tcccttagtt gagccgcagc ggatcatttt
    23401 ttcagccatg cttaacgcat aaacaaatcc tgagcacgca gcactgatat caaaagcgat
    23461 agcatgagtt gctcccaatt tcccttgtac ttgtgctgct acagatggtg tcgtgtaatc
    23521 gggcgacatt gttgctacta aaataaaatc caaatcttta gggtttagat ttttctgttt
    23581 tatcaatttt tccgccacta aaatgcataa atcagaagtc tcctgatttg ttacacatct
    23641 tcgttcacga atacccgtcc ttgacgaaat ccattcatca gaggtatcca tcatcttgga
    23701 aagatcatta ttagtaataa tcttttcagg aaggacgctg gctgttgcag taatcattcc
    23761 aaattgttcc attaaatatc ctcacttata gtcttgcaag aagtcgtgca agtttttcag
    23821 tgcatccagc aaggcatgct gttcctccgt agccattccg tctaagatgt tcttgaccat
    23881 ttcacgatga aaatgctgat ggacccgaaa tagtaacttt ccttttttcg ttagtcccaa
    23941 ctttacaacg cggcggtcat cttcactgcg gatacgttcg acatagcctt tttttactaa
    24001 cttgttaata gcagtggtca aggtcccaac agtaattgat agttctttgg ctacttctga
    24061 agttgttttt ttcttgtaca tcccgattgc ctcgatcgtg tgcatctctg tgattgacag
    24121 atctttgaat tgagattttt tcagctcaga ctcttcgatt gtcaaaatat cattgaagac
    24181 actaacaaga taatcattta ctgtttttaa atctggttcc atgttccacc gtccactaat
    24241 tagtttgatt atcaaatctt ttgatgttca aactatatct taactttttt caaaatgcaa
    24301 ccttaaaaga tcattttttt actatatgag ctttttttaa gattttactc atttaaaaca
    24361 actgataaat cag
//
LOCUS       DACDTO010000035        24363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDTO010000035 DACDTO010000000
VERSION     DACDTO010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.035"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            625..1953
                     /locus_tag="IWK70_001442"
     CDS             625..1953
                     /locus_tag="IWK70_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9613976.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2155..2988
                     /locus_tag="IWK70_001443"
     CDS             2155..2988
                     /locus_tag="IWK70_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9613977.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3004..3741
                     /locus_tag="IWK70_001444"
     CDS             3004..3741
                     /locus_tag="IWK70_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9613978.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3896..4822)
                     /locus_tag="IWK70_001445"
     CDS             complement(3896..4822)
                     /locus_tag="IWK70_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9613979.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5097..5930
                     /locus_tag="IWK70_001446"
     CDS             5097..5930
                     /locus_tag="IWK70_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9613980.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5964..6803
                     /locus_tag="IWK70_001447"
     CDS             5964..6803
                     /locus_tag="IWK70_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9613981.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6829..7389
                     /locus_tag="IWK70_001448"
     CDS             6829..7389
                     /locus_tag="IWK70_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9613982.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7588..9309
                     /locus_tag="IWK70_001449"
     CDS             7588..9309
                     /locus_tag="IWK70_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9613983.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9474..10616
                     /locus_tag="IWK70_001450"
     CDS             9474..10616
                     /locus_tag="IWK70_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9613984.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10632..11843
                     /gene="thiI"
                     /locus_tag="IWK70_001451"
     CDS             10632..11843
                     /gene="thiI"
                     /locus_tag="IWK70_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9613985.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12416..13063
                     /locus_tag="IWK70_001452"
     CDS             12416..13063
                     /locus_tag="IWK70_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9613986.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            13456..16101
                     /locus_tag="IWK70_001453"
     CDS             13456..16101
                     /locus_tag="IWK70_001453"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9613987.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16411..17724
                     /locus_tag="IWK70_001454"
     CDS             16411..17724
                     /locus_tag="IWK70_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9613988.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17714..18367
                     /locus_tag="IWK70_001455"
     CDS             17714..18367
                     /locus_tag="IWK70_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9613989.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18422..18973
                     /gene="radC"
                     /locus_tag="IWK70_001456"
                     /pseudo
     CDS             18422..18973
                     /gene="radC"
                     /locus_tag="IWK70_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            19104..20189
                     /locus_tag="IWK70_001457"
     CDS             19104..20189
                     /locus_tag="IWK70_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9613990.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            20186..22078
                     /locus_tag="IWK70_001458"
     CDS             20186..22078
                     /locus_tag="IWK70_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9613991.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            22085..22462
                     /locus_tag="IWK70_001459"
     CDS             22085..22462
                     /locus_tag="IWK70_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9613992.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            22616..23398
                     /locus_tag="IWK70_001460"
     CDS             22616..23398
                     /locus_tag="IWK70_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9613993.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(23524..24255)
                     /gene="erm(A)"
                     /locus_tag="IWK70_001461"
     CDS             complement(23524..24255)
                     /gene="erm(A)"
                     /locus_tag="IWK70_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9613994.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(24313..>24363)
                     /locus_tag="IWK70_001462"
     CDS             complement(24313..>24363)
                     /locus_tag="IWK70_001462"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
                     /protein_id="HAP9613995.1"
                     /translation="FSIFVINKVRYQPNQN"
BASE COUNT         8343 a         3936 c         5049 g         7035 t
ORIGIN      
        1 gtcgtaggtt cgagtcctac tgccggagta aataaagctt atgcaaagaa aaagaaatcc
       61 aatcacttgg gtttcttttt cttttattat tatgatgaat caagctttct gtttttgtca
      121 cgtatgttgt aatctttacg aaagagtaga gttggctact cataatcaac catacttccc
      181 atcatcgcta aatacgaaat aaatcagcat tcctacacca atcaaagcac aagctgcacc
      241 aagaaaaggg atgacattcc caaagttatc tgtattcata agtatgccac cacccacaaa
      301 caaaaagccg ccagcaatca ggtttttcat tttattcatc tcctttctta cgttatagta
      361 tttccttctt tcaatgagta ttttgaaagc tatcttattg tatgatgttc ttctaggcct
      421 cttctttatt atacaggcca ttttcgttat ctcatagaaa tattcctttt tatctgaaaa
      481 ctattgtata ccgtatgaca tttttcttca tgaagttgta agtatattct cattcaaatg
      541 aaaatttcat catagaagag attgtatttc taaattctta agagtaaaat acgaagtata
      601 acttggattt ggaaggatga ttggatgaaa gctattacgc ctgatatgac aacaaaattt
      661 aacaaacagt ttcaagcaaa tatcaaacag caggcattgc agcgggcagt tgtgaagaac
      721 ggaattactg cttctgctga aaatgtttca gcacatgtgg agaatacacc ggtattttct
      781 atagatttgg ctacggggaa agtggctaat caaatgcaat cggggcgttg ctggatgttt
      841 gctgcactga acacattccg tcataagatg ctgaatcaat tccatttaaa agagtttgaa
      901 ttatcacaaa attacacttt tttctgggat aaatatgaaa aggcaaatta tttctatgaa
      961 aacatcattg caacaggaaa agaggcaatc gatagccgga aagtagcttt cttactcgct
     1021 actcctcaac aagatggcgg ccaatgggat atgatcgttt cattattcca aaaatatggc
     1081 gttgtgccta aatcggtgat gccggaaagt gcgaatagtt caaattctcg agatttgaat
     1141 aactacttga acaaattact gcgtaaagat gcagtagtgt tacggaaaat ggttgctcaa
     1201 ggagaaacat tagacgctat tgaagaaaag aaagaagaaa tgctagaaag catttataac
     1261 ttcttagcta tctcattagg aacacctcca aaagaattcg attttgagta ccgcgatgaa
     1321 gaaaagaact atcatcttga ccgaggactg acaccacaaa ttttttatga taaatacatc
     1381 ggtgtaaaat tagatgatta tgttagcgtg atcaatgcac caacaaaaga caaaccattc
     1441 aatcgttctt atacagtaga gatgctagga aatgtcgttg gcgggaaaga agtcaaatat
     1501 ctgaatgttg atatggaaac tttcaaaaaa ttagcgattg ctcagcttga agaaggagaa
     1561 tctgtttggt ttggttgtga tgtaggtcaa tcttctacta gaactagtgg catcatggcc
     1621 ttagatgctt attcaatgga tgatctattt gatacggatt ttacaatgac taaagctgaa
     1681 cgtttagatt ttggagaaag tctgatgacc catgcgatgg tattgaccgg tgttgactta
     1741 gtagacgggc aatcaacaaa atggaaagtc gaaaatagct ggggcgaaaa agttggaaag
     1801 aatggcttct ttgttatgag tgatgcctgg atggacgaat atacgtacca aatcgttgta
     1861 agaaaagatt tattgacgaa agagcaatta aatgcgttca atgaagaacc aattgttcta
     1921 tctccgtggg atccaatggg tgctttagca tagacgagta tttcaatgaa gagtatgtag
     1981 taaagataaa aagagctggc ttaagccagc tctttttatc tttaaagtcc attccctgtt
     2041 ttttatggtc cctgtgctat aattctaaat aaaggataag gattggaaat aatcctttta
     2101 tcattaaaaa tatgatgacg aagtagaata aaattgggag taggggggaa agtcatggat
     2161 atccaaactg ttttatctga tctgaaatta aatggtaaag tgattccagt tgttggtggc
     2221 gatgttaatc agacctatcg tataaaaacc gaacatcgtg cttatttttt aaaaatccat
     2281 ccgaatgtaa aaaaaggatt ttttgaagca gaagtagacg gattaaaaga actgtcagca
     2341 ttcgtacgtg taccggatac gtatatgcta ggtgaaacaa gtgaaggcgc ttatttgtta
     2401 atggaatgga tcgagccggg aaaaggggat caacgagatc ttgctgcagc tttagctaat
     2461 ctccatcagc aaacagcgcc acaatttggt tttcgtaaag ataactattt gggtacactt
     2521 gttcagaaaa attcttttga agaagattgg tggacttttt tctttaaaga tcggcttgaa
     2581 tcccagattt ctttggcaga agaaacgaat cgatggaatg tacagcgtca agaaaagtat
     2641 ctgcgattta aagaacgtgt attgaaatcg gttgaaccaa aaaagatcac tccacgcctc
     2701 ttgcacggag acctgtggag tggtaatgtg ttctttgacc agcaagggca tccagttttt
     2761 gtcgacccag ctgtttctta tgggaatcgg gaacaagata ttgcaatgag ccagttattc
     2821 ggtggtttcc gaccggaatt tttagatgct tatcaaacta tatttccatt agaaaaggga
     2881 tggaaagacc gactgccgat ttaccagtta tattatcttt tggcacattt gaatatgttt
     2941 ggagaaagtt acggttccca agtagatcaa ttactagaaa atttttaaga ggagggcgtg
     3001 tttttggcaa aaaaatatac caaaaagcat gaagtttcat attatgagtg tgacatcaac
     3061 caaacgatga catttccttc gatgttagga gtagttatca aaacgtctga agatcaaagc
     3121 gaccagttgg gaagaggcag tgaattcgtc aatagctttg gacttacttg ggtcatcacg
     3181 aattattcca tggagattaa tcgtctccct agagtcggtg aagtaatctc agtaacgact
     3241 caagccatgg agtacaataa gtttttctgt tatcggaact tctggatcca tgatgaagat
     3301 gggaaagaat tagtcaaaat agaatctgtt ttcgtattga tggattttgt gaatcgaaaa
     3361 atgagcagtg ttaatgaaga gatcattgct ccttttgaga gtgagaagat caagaaaatc
     3421 aagcgtcagg aaaaaattga aaagattgat tcgggcttca tgttgccata tcgtgttcgt
     3481 ttttatgata tcgatagtaa tcagcatgtt aataatgcga tgtattttaa ttggatcatt
     3541 gacgtattag gatatgactt tttgactacg catgttccag aaaaagtaat tattcgattt
     3601 gataaagaag tagagtacgg gaatgagatc gaaagccatt ttgaacaaat ccctatcgaa
     3661 aatggtgtca aaacaaaaca tgaaatccga atgcaagaac agctgtattg tgaagcaaat
     3721 attatatgga aaaataaata aaaaagaggt tgtgacagaa gggggcaact tcaagaaata
     3781 agaagaaatt tacgaaaatt gtttctcaaa tttttgtaaa ttttcgactt agttttcgaa
     3841 gaagctattt ttgtcacagc ctctcttttt aagtttagct attagataat gatgtttagt
     3901 tttggataaa cgaaatcaat tcttcacccg acaaaccata gtttacagca atcaatagtt
     3961 ttaaacgtgc tttttgacta ttaatactgt tacagaaaat aatccccatt ttttctagtt
     4021 cttttccccc gccttcataa tcataaacag gctctgcgat tccattgaaa caacgagaaa
     4081 caagtactac agggattttt tggacaagaa gcttttccaa agcagctaag gcttgtggag
     4141 ggagattccc cgcacctaaa gcttcgatta ccaaaccgtc tactttcgta tgggcgatgg
     4201 cttccaataa gtccccttgc atcccagcat atgctttaac gatcgggata gtcccatcta
     4261 cagcggaaat gtccagtcgc tttgtttcta aaagttcctg caagaataaa attcgattct
     4321 tagttactaa gccgatggga cccaatgtag gtgttcggaa agtagcaaca tttgtggtat
     4381 gggtctttgt cacgtagcgt gcagaatgga tctcatcgtt catgaccacc aaaaccccct
     4441 tatctagcgc ttcttcacaa cttgcgacac gaatcgcact ttcaaaatta taaaggccgt
     4501 cacttccaag ttcattactt gaacgcattg ccccagtcaa aacaattggt aatttgttgc
     4561 caatcgttgt atcaagaaaa aaagcagttt cttctaatgt atctgtaccg tgtgtgatca
     4621 ccactccgga tatacctgat gcataagcgg acaagatacg ttcttttaat accaacatgt
     4681 gagaaggcat gatgtgtggt gagggaagat tgaagatatc ttcaactaat aattctacat
     4741 gcttggggat tgtgatttct gcatctaata atgggtttgc cacatcagga ctaacaccgc
     4801 ccgtgagcat gttttctttc atggcgatcg ttccgccggt gtgtagtaca agtatttttt
     4861 ttcattttac agacttcctt acgaataatt ttgcatagcc gttaaatctt attgtatact
     4921 atataaaaga gttttgctat cagtgaagaa taacgaatag cctaacttat cataaaaaag
     4981 ctgaccttat taaaatggaa aagactcaac cttgacctcc taatacaatg gagtatctgg
     5041 ttgagtttct tttacaagaa agcagccaag gaaatcaatc acgaaggtgg ccggaaatga
     5101 tcagaatcgg attaacatcg tttaaagaac atgacaaatt aactggtaaa aagaataaca
     5161 ctctttatga atatgcaggg tttctaccct tagtggaatt agatactgcc tattatggaa
     5221 ttccccgaaa gtcctcagtt gagaattgga taagtgaagt acctgaatca tttcgctttg
     5281 tagtcaaagt ctatcaagga atcagcggtc aaggagactg gaagccttat tatcagaccg
     5341 aagaagagat gattgcgcat tttttagatg ttatgtcgcc aatggtagat gctggaaaga
     5401 tgttttgctt tttagtccaa ttcccctcat cattcaaatg tacgaaagaa aatgtccagt
     5461 acttgcgcaa acttagaaag tggtttgagg gttatcctgt ttctatagaa ttgcgggatt
     5521 attcttggta cagcgatgaa tttcttgaac gtacaagaaa ctttatgtct agagccgctt
     5581 tttctttagc catcatcgat gagccacagt tgctgaatac aacgattcct tttgatccat
     5641 ttgtcaccaa tccgaacttt gctctttttc gattccatgg taggaatgcg caaggatggc
     5701 aagcaaatga taaagagtgg cgtaaaaaaa gaacgttata tcgttattca gaaaaagaac
     5761 tgcaagaact agcacatgaa gtcaaagtag ttgattctaa gacaaaagag gtaggtgtga
     5821 tttttaataa taattctggt ggtgatgcgg ctgaaaatgc attagcactg caaaaaatat
     5881 taaatatcaa gccagatgga ctcaatccaa cccaaatgga tctgttttaa gggcttattt
     5941 tattcaagaa aggggcgaag tctatgatca aagctatttt ttttgatatt gacggaacat
     6001 tggtcaccaa tcgttcaaaa gcgttggaat caacaaaaca agcaattgaa tatgctcgga
     6061 aaaacggcat tttatgcggt gtagcaacgg ggcgcagccc agtaaagatc aaagaaatca
     6121 ttgatgaatt ggaattagat atgtatgtcg tgtataatgg tcaactggtt tttactgcag
     6181 atcgcacgat tatcgaccac ccttttgaac aaaaagtatt ggaacatatt gttgaatttg
     6241 cagatgagaa ccatcgacaa atcgtttttg gtgcgagaaa tcggttagat ggatcaacga
     6301 cgatgctatt aggacaatcg atttttatca agcgactagt cagcttttta ccgcgtaaat
     6361 tcccggtacg tctgatgaaa aaaatcctcc aggtttttag ccctcaccga caaaaggatc
     6421 gttatgaaac gttagagata ttaaaagaac caatttatca atgtatattg ctcagtccgg
     6481 agtcagaaca gaaaaagtta gaacatcgct ttcccgagtg tacgtttcag cgttcaaata
     6541 cgtatactgt agatatcatc ccaaaaggtg gttcaaaatt actaggcatc caagctttcg
     6601 caaatgctgt cgaaattgaa atggaagaaa ttatggcatt cggcgatcat tacaatgata
     6661 tcgagatgct gaaaggtgta ggaattggag tggccatggg gaatgctcag atcgaagtaa
     6721 aacatgctgc agactttgtg actcaatcaa atgaagaaga tgggatatac gaaggactga
     6781 aacacttcgg tctgatttat tagtcaatga aagaaggaaa aaatcagtat ggaagcagag
     6841 acaccttttc aaaaagtaga aacattccac aaaacttttg atccaagaag accagagata
     6901 cccacggctt ttacagaaaa tgaagctcat acacgtgctg cgttcaaaat agaagaactt
     6961 gttgaatttg ttgcagctac ttcacctaat aaagaacaac tgcatgaagc tgttatgctg
     7021 ctgcacgaag cattagataa agcagaagaa aaaatacttg gtaaaacaca gtggggaagt
     7081 gatccgctgg ttgaacaagt agatgcactg acagatatcc tttactttac ttatggttct
     7141 ttttcattac taggaatagt gcctgatcga atattcgaaa tcgtccatca ggcaaatatg
     7201 gggaaattat ttccagatgg aaaacctcat tatgaccctg taacaaataa agtgttgaaa
     7261 cctgaaaact ggcagatgga atatgctcca gaacctaaaa tcgcggcaga aatccaacgt
     7321 cagattcaag aagcgattgc taagaagaaa tccgctgata taggcatgtg cacaaatgaa
     7381 aaagagtgat cagagtttca ctatttttgt tatctttata cgatggaaaa acaaaagatt
     7441 ttcgtttaag ttagtgaatg catgaaaata gtatgctata atctagaata gatgtctttt
     7501 gcacgagaga cgtattattt ggatttatgc caagcagaac gccatctgtt gcataaaaga
     7561 tgcatactga aaacggagga accataaatg ggaaataatg ttatcatcgg aatcgtcatt
     7621 gcaatcatta tagtcgccat tgtcgtcttt ttggttagtt acttgatgaa gaaaaaaaac
     7681 caagatcgat tggatgcctt agaaaaacgc aaggaagaat tatttgactt gccagtgatt
     7741 gaagaagtcg atgatgttaa aaaaatgcat ctggttgggc aaagccaaaa tacctttcgt
     7801 gaatggagtc aaaaatggaa tgacatatct acttcctcgt tcgctgaact agaaagtcaa
     7861 attttcgaag tggaaaacct caatgaaagt tttcggttct taaaaggaaa aaaagcagtt
     7921 gttgaagcag aagctacaat ggaccaaatg gagaaacaag tgacggaaat ccgtgaaggt
     7981 ctaaaggaat tgcgggaaac ggaagaacgc aactcactag ctgttcagca ggcacttgac
     8041 gtctatgaag aactgaaaga aaaagtcaga gaagacgagc atgaatacgg accagctctt
     8101 tttgaattgc aaaaacagat caaagtgatc gaaaaagaat ttacgcagtt tgtgacttta
     8161 aacacttctg gagaccctgt tgaagcccgc gaggttttgg ataaagcaga aaaacatacg
     8221 tatgaagtgg aagctctgat gaaaaaaatt ccaccgctgt atgaggagct ccataccact
     8281 ttccctgaac aacttgaaga aatcagtgat acttacgaga aattgatgga agaacaatat
     8341 gtattcccag aagaagatct tgctgaagat atcgcaaaag taagtcgaag aattgaaaat
     8401 tcattagcca atcttgaaaa aacagaagtt gaaacggtcg aatttgaaaa ccgtgaaaca
     8461 gctgatttga ttgattcgtt atatgatatc ttagaacggg aaatggaagc tcagagatat
     8521 gtaaaaacta accaatcaac gattgcagag tatatcaaac atacaacaaa gaataaccgt
     8581 caattattga ttgagctgga tcacacatct caaagctata cattgaacca caatgaaatt
     8641 ggccgtgtac gaggtttcca aaccgaagtg gaagaaatgg aacgtcaaaa tgaacagatg
     8701 attccacaga tccgtcaaca cgaaattcct tattcagaag tgcgcacgtt ctataaaaca
     8761 gtcttcaaag tcttagaaga tatcgaaaag caacaagtag agatcgacga ctcattacat
     8821 gaattgagaa aaggcgaaaa agaagcgcaa gaaaagatcg atacattcga attcaaattg
     8881 cgcagtttga aacgttttgt agaaaaacaa cgtttgcctg gcttgccgaa tgattaccta
     8941 gaattctttt ttgttgctac tgaccgtatt gaagaactaa gtgttgtctt gaataaaatt
     9001 cgagtaaaca tggaagaggt caatcgttta gttgctctat gtgaagaaga tctagaatta
     9061 ttagataaga agacacatga tttagtagat gcagcagcat taacggagca aatgctccaa
     9121 tatgcaaatc gttatcgtca tacacacgaa gaagtgagag aagcaattga taaaagctac
     9181 tatttattca ataaagaata tcactatcaa gaagcattag acgaaatcgg tacagcatta
     9241 gagcgagtag aacctggtgc tttcaaacgc atcgaagatt tttattttaa tcatccagac
     9301 ttagtataaa gcaaaaaagt tagtaaacca gcaagagcaa gacggattat tgtcttgttc
     9361 ttgtcttttt ttgtatattt gaaaattgaa acaagtgaaa atttcacctg ctcttgaaag
     9421 caccttggac aaccgttata atagaaaaga atttcaaaaa agaggggttt cttatgatat
     9481 attttgataa cagtgcaaca accccaatct ttccgcaaag cttagatgca tatgtaaaaa
     9541 ccagtcagcg gattattggg aatccatcaa gcttgcatga tttaggcaat caagctaacc
     9601 ggttgttgca gcaagcaaga aagcagatcg ctgatttgat ccatgtagaa ccagaagaaa
     9661 tctattttac ttcaggcgga acagaaggag ataactgggt attaaaagga acgatgatcg
     9721 aaaaaagaga atatgggaat catatgatca tttcaggagt cgaacatccg gccgtcagcg
     9781 agacagcaga acaattaaaa gaattaggaa tcgaagtaag tatcgcccca gtggataaac
     9841 gcggatttgt acgtgtcgac gaattgaaag aattaataag aaaagagaca gtattagtct
     9901 ctgttatggc tgtgaataat gaagtaggat cgattcagcc catccaggaa atcagtgatc
     9961 ttttggatga ttttcctaaa attcatttcc atgtagatgc tgttcaggcg attggaaaag
    10021 taccattctc gcaatggttg acagaaagag ttgattttgc aacattttca gctcataaat
    10081 tccatggccc acggggaact ggtttcattt attggaaaaa gggtcgtcgt ttagcaccat
    10141 tactgacagg tggagggcaa gaaagaaatc agcgaagtgg gacagaaaat gttccaggta
    10201 ttgtggctat ggttaaagct ttacgtttat cattagagat aaaggaacaa aaacctgagc
    10261 agacacaacg cttaaaagta gctctaatca atgcgttgaa gacgtatgag aaagtgacga
    10321 ttttttcaga aggaactgac tatgcaccgc atattttatg ttttgctcta aaaggaattc
    10381 gcggcgaagt tctcgtccat gccttagaag aaaaacaaat atttgtttct actactagtg
    10441 cctgctcctc acgtaaacat atggcaagca gtacattgca cgcaatgcat gtaccaaatg
    10501 atctggctac atctgctgtt cgaatcagct tagatgaaaa caattcgctt gcagaagtcg
    10561 agcaatttat gattattttc aagcagcttt ataagaaatt ttctgcaatc aattaaaaga
    10621 ggagtgttct tgtgaaatac agtgaaatta tggtccgtta tggggaactt tcgacaaaag
    10681 gaaagaatcg aaaaagcttt atcatgcaac tagcacaaaa tgtcagacaa gctttgaaag
    10741 actttccaga aatcaaaatc catgctgatc gggaccggat gcatctttta ttgaatggcg
    10801 cagacgggca gctagttatt ccaaaactag caaaaatatt tgggatccaa aatttctctc
    10861 caagtattcg tgtagaaaaa gatgtcgaag ttttgaaaca agctgttcaa gacatcatga
    10921 aagaaattta tactgggaaa gaaacattta aaattatcgc taaaagaagc gatcaccaat
    10981 ttgaattgac aagtaatgaa ttgaatcaaa cattaggtaa tgctgttttt gatattttcc
    11041 cgcatatcca agttcaaatg cgtcaaccag atattccatt acgtgtagag attcggcgtg
    11101 atggcgctta tctatcgtat gaaacgatca agggagctgg aggattgccc gtaggaacaa
    11161 gcggaaaagg aatgcttatg ctttctgggg ggattgattc tcctgtagca gggtactttg
    11221 ccatgaagcg gggagtggaa attgaagctg ttcattttgc tagtccgccg tatacaagtg
    11281 aacaagcact gcaaaaagca aaagatctaa cagccaaact agcaccttat gtgggaacga
    11341 tccattttat cgaagtgcca tttaccgaga ttcaagaaga aatcaaaaag tcagtgccac
    11401 aaggatattg gatgacaatt actcgtcgaa tgatgttgcg tttgaccgat gaaattcgcc
    11461 gtattcgtca tggtttagtc attttgaatg gtgaatcatt aggtcaagtg gcttcacaaa
    11521 ctttgcaaag tatggtggct atcaatgaag taacgaatac gccaatcatt cggcctgtag
    11581 cgacaatgga taaacttgaa atcattgaag tagcagaaca gatcgataca tttaatcttt
    11641 ctatccagcc ttttgaagat tgctgtacga tttttgctcc tccacagcca aaaacacgcc
    11701 ctcaactgga aaaggtccat caatatgaag agcgtttagc aatagatgaa atgatccaac
    11761 gagcactagc tggcttgaag atggaagaga tacaagcaga atcgtctaat gaaaaaactg
    11821 aagaattttc agaattgcta taagaggtta tgaaaatagt gttttgacca gagtattaga
    11881 gaaacaaaac gaaaaaatag ctccatgtat tttaccgttt ttgaactaat gtcgaagatt
    11941 agctgattga ataccattta tcaagaagtt atggaaaagc cggttctttt ttactaactg
    12001 aaaaataaaa ttttatttag agatcaagct acagttgata aatgatataa aaaagagaat
    12061 ttcccatgaa atagcgaggt tgtgacagaa gtgggcagct tcaagaaata agaaggaatt
    12121 tacgaaaatt gcttttcaaa tttttgtgaa atttcggctt atttttcgaa gaagttactt
    12181 ctggaacacc gtgtatcaga ggttgcgaaa aagctgccct gcatcaagta ataagaacag
    12241 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
    12301 cttttgaaca ccgttttatt tgtctcacac ccttttcctt cctacttgta accttgctcc
    12361 cgtgttagaa acatgttaca ctaaatatgt gaaattatta acaaggagcg tcgttatgcg
    12421 agaaaaaaca gatacactgc caaaagcaac tgcgaaaaga ttacctttat atttacgcta
    12481 cttgaagatg ctggatgact caggtataag tagaatcaaa tcaaatgagt tcagcgaaat
    12541 cacccaaatt ccttctgcaa cgatccgcag agacttctcg caattaggcg agctaggcag
    12601 aagcggatat ggctatgatg tcccttttct tatcgatgta ttcaataata tccttaatac
    12661 taaagaagaa aaacgaatcg cattagtagg atatggaaat cttggcaaag cattgaaaca
    12721 caacaatttt agaagaaatg aaaatctaaa tatcgtgtgc gtctttgata atgatccagc
    12781 actgatcaat cgagtaattg atggagaaat gatctatccg attgatcgtt tcgcggagat
    12841 agccaaagca aaaaatgtga cagtagcaat ctctacggta ccaagtaaat attcacaatc
    12901 tgctattgat gaaattgtca aaggaaatgt aaccgctata cttaattttg cgcctgacag
    12961 agtaactgtc ccagcttatg tcaatgttca atacattgat ttgacaaccg agttgcagac
    13021 gcttatttac tttgatgaga attactcaga agttttttct taggaaaatt tgagtaggct
    13081 tattgatctt gcgagaactc gtcgtaattc tttcaaaaat tttttagtac taaatgaagt
    13141 ggttttttca cgaccacttg ttgactaaaa aaaaaaacca ttgtagaatg aagcataacg
    13201 ctgaacaaga ggagtaactg gcacattcat tcttagagag agaactatct gctgaaaggt
    13261 tctcaatgac aaccagtgaa ggtagcttgg gagtttctgt cttgaatgga gtagagatag
    13321 acgagtcgtg atcgttaact cacttgagag gcaataatga ttgattattg caatttaggt
    13381 ggtaccgcgt aaatcatacg tcctagaata cgaaagtatt ttaggacgtt ttttagttta
    13441 aaggaggaaa acagcatgac ggaagaaaat aacctatcaa caaaatataa tccgcaagaa
    13501 gttgaagctg gacgttatca gcattggtta gaacaagatt tgtttaaacc gagcggagat
    13561 aaaaaagcaa aaccttattc aatcgtaatc ccgcccccaa atgttactgg aaaacttcat
    13621 ttagggcatg cttgggatac gaccttgcaa gatatgctga ttcgtcaaaa gagaatgcaa
    13681 ggatatgata ctttatggct gcctggtatg gaccatgcag gaatcgcgac acaagcaaaa
    13741 gtagaagcga agcttagaga gcaagggatt tctcgttatg atctaggtcg tgaaaagttc
    13801 atcgatcaag tgtgggaatg gaaagaagag tatgcgagcc acatccgcga acagtgggct
    13861 aaaatgggct tatctttaga ttacagtagg gaacgtttta cattagatga tggattatct
    13921 gaagcagtac gtaaagtttt tgttacctta tacgaaaaag gtttgattta tcgtggtgaa
    13981 tatatcatca actgggatcc tcaagcacga acagctctat ctgatatcga agttattcat
    14041 aaagatatcg aaggcgcctt ttatcatatg agctatgaac tagcagatgg atcaggcgta
    14101 gtagaaattg cgacaacccg tccagaaacg atgttgggag atacggcgat tgctgttcat
    14161 ccagaagacg agcgttatca agcgctcatc ggcaaaaaag tgattctacc tttagtcaat
    14221 aaacaaatcc caatcattgc agatgaatat gtagatatgg aattcgggac aggtgtagta
    14281 aaaatcactc cagcacatga tccaaacgac tttgaggtag gtaatcgtca tgatcttcca
    14341 cgcgtaaatg ttatggatga tgatggtacg atgaatgaac tagcaggaaa atatgctgga
    14401 atggaacgtt ttgctgctcg taaagcaatc gtgcaagatc tgaaagaaat cggtcgattg
    14461 attaaaatcg atacgatgat ccacagtgtg ggtcattccg agcggacagg tgtcgttgtt
    14521 gaacctcggc tgtctactca atggtttgta aaaatggcgc cgttagcgaa aaaagccatt
    14581 gataaccaag aaacagatca agcagtggaa ttcttccctc caagattcaa tcaaacgttt
    14641 ttacgttgga tggaaaatgt ccatgattgg gtaatctcac gacaattatg gtggggacat
    14701 caaattcctg catggtatca caaagaaaca ggtgaaatgt atgtagggat ggaagcacca
    14761 gcggatagtg aaaactgggt acaagatcct gatgttttag acacttggtt tagttcagct
    14821 ttgtggcctt tctcaacaat gggctggcca gatacggaaa gcgaagatta taagcgatac
    14881 ttccctacta gcactttagt aaccggttat gacatcattt tcttctgggt aagcagaatg
    14941 attttccaaa gcttggaatt tactggcgaa cgtccatttg agaatgtctt gattcatgga
    15001 ttgattcgtg acgaacaagg gcggaagatg agtaaatcat taggtaatgg aatcgatccg
    15061 atggaagtaa tcgaaaagta tggagcagat gctctccgct ggttcttatc gaatggttct
    15121 gcaccaggac aagatgtccg cttcagctat gaaaaaatgg atgcttcttg gaactttatc
    15181 aacaaaatct ggaatgcttc acgtttcgtt atcatgaata tcgaaggaat gaccgtagaa
    15241 gagatcgatt tatctggaga aaaatcagtg gctgaccggt ggatccttac tcgtttgaat
    15301 gaaacgatcg aacgagtaac tgagctattt gatcgttttg aatttggtga agcaggtcga
    15361 caactgtata actttatctg ggatgatttt tgtgactggt acatcgaaat gagtaaagaa
    15421 gtactttatg gagatgatgc tgtttcaaaa caaaccaccc gtagcatctt agtccataca
    15481 cttgatcaga tcctacgttt attgcatcca atcatgccat tcgttacaga agaaatatgg
    15541 gaacatattc ctcatcaggg aaattctcta gtagtggcag aatatcctgt tgtacgtcca
    15601 gagtttgatg atgaaacagc ttctaaaggg atggaagtat tgaaagaatt gattcgagcc
    15661 gttcgaaata ttcgttcaga agtgaataca ccattgtcta aaccaattac gttgctaatt
    15721 aaaacaaacg atgctaaaat cgatcaattt ttagttgaaa atacaagcta tatcgaacgc
    15781 ttctgtaatc ccgaagagtt gacgatatca agtgagatca ctgcgccaga tctagcgatg
    15841 tcagctgttc tgacaggtgc agaaatcttc ttgccgttag caggattgat caatattgaa
    15901 gaagaaatca aacgattaga aaaagaactg gaaaagtgga caagcgaagt aaaacgtgtc
    15961 caaggaaaac tttctaatga acgttttgtt tctaacgcgc cggaagaagt agtagaggca
    16021 gagcgtgcaa aagaaaaaga ttatttagag aagcaaacgg ctgtcaaaga acgaatcgaa
    16081 agtttgcgta ctattcaata agaatagaaa aagagagtgt gaaatcagct agctattact
    16141 aaaagctagc tgattgaaca tcgttacagg aaagagtttg agcctcactc ttagcttcga
    16201 ttcataagga gatgtgacat aaattttgtc gcatctcttt tttagtctac tcttattatg
    16261 taggagaagg agggaggtgg atcatgtatc aagagtcaat aaagtagttg ataaaatcgt
    16321 tcctctctct gtttcttttc tctttgtcat acttttattt ttcttatgtg ttctatttgt
    16381 tataataaaa gagaaatggg ggaattgttg atgacgattg aagaagcaat agaatggatc
    16441 catagtcgat taccatttgg ttcacgtcct ggccttgacc gagtcgaagc tttattagaa
    16501 gtggtagggc atccagagag aaaagtaaaa acgatccata tcgctggaac aaacggaaaa
    16561 ggttctactg tagcttattt gagaagccta ttagaagagg ctgggctgac agtaggtaca
    16621 tttacttcac cttatatcga aacgttcaat gaacggatcg caatcaatgg caaaagcatt
    16681 ccagacaatg atttgatcca gcttgtaaaa aaatatcagc cactgatcaa acaacttgat
    16741 caatttgaag cagtagctgg tattactgaa ttcgagacat taacagcaat ggcatttgat
    16801 tattttttga tgaagaaagt ggacgtagcg attatcgaag ttggcttagg cggcttattg
    16861 gattctacca atgtagctgt accaatgctg acgggaataa caacgattgg acttgatcat
    16921 accgaaatac ttggagatac aattgaagaa atcgctgcac aaaaagctgg aatcatcaaa
    16981 caaggcgttc cagtagtaac aggaaatatt gccccagaag cttttactgt catccaacaa
    17041 acagctaaag caaaaaatgc accgctttat gtatacgggg aaaattataa aataaactat
    17101 ctgcatcctg atgagatgtg gggagaagta tttgaatttt ttaatgaaat aggaaagatc
    17161 cctcaattaa ccactccgct tttagggcaa catcaagctg aaaatgcagg agtagccatc
    17221 gaattgtatt ttgtttactg tcaattggaa aagctgcctt tccgagaaaa agatgttttg
    17281 aagggattga agaaatctcg atggccagga agaatggagc gtttgagtaa cgagccatta
    17341 gttgttttag atggtgccca taatacgcat gcaatgaacc ggcttgttga gaatttgaaa
    17401 aaagaattca aaggatatca aatcaacatt ttgttttccg ccttacgaac taaaaatgtt
    17461 agagaaatgc tagaacgatt gcagcaagta ccaaatgtcc atatttattt gacgaccttt
    17521 gaacatccaa aagccatcga attaagtgaa gtatcagatt tagcaagtga gagagtatca
    17581 atcgtttcat tgtggcaatt cggattagca gatatattgg aaaagatgac aaatgaagac
    17641 gtattagttg tgaccggttc actttatttc atttcagaag ttcgccattt attaatacaa
    17701 ttaggaggag atcatgaagt ataatgcagt gatttttgac atggatggtt tgctgttcga
    17761 cacagagtta gtgtattacg aagccacaca agtagtagca gacaagatgg gttttcctta
    17821 tgataaagac ctgtatttaa aattcttagg tgtttcagat gaagaagtat gggcgaatta
    17881 tcatatgatt ttctcggaat ttggcaaaac aagtgtacaa agattcatag atgattcata
    17941 ccaggaaacg cttgaacgtt tttcgcaagg agcggtacaa atgaagcccg gtgtgttgga
    18001 actcctctct tttttagaag aacatcagat tcccaaagtt gtcgcttcca gtaaccaacg
    18061 agcagtcatt gaacttttat tgaagaagaa caatctgttt gaacgatttg agacgattgt
    18121 ttctgcagaa aatgttcaac gagcaaaacc agacccagaa attttcttgt tagctaatca
    18181 gaagctaggt acagcgaaga aagaaacatt gattttagaa gattcacaaa atggtatttt
    18241 agcagcttct gcggctgaga tcccggtagt catggtacca gatctgcttc caccatccca
    18301 agaattgatc aaaaaaacag ctggggttgt ttcttcttta catgaaatcc ctgcattgtt
    18361 aaaataaaaa tccatctctt ttcgtatctt attttagata agatagaaag gagatgtttt
    18421 tttgggaaaa cgattgatca aagaagtacc tactagctct ttaccaaggg aacgtatgga
    18481 aatttatggc gcggaagctt tatcggatca ggagcttttg gcaatcttac tgcgtaccgg
    18541 tcaacaccct tatagtgtga tgtcgattgc tggaaatctg ttaaaaacgt ttggaggact
    18601 ggcctcttta cgacaggcta ctttgcatga gttagaagag attcgaggga taggacgcgt
    18661 gaaagcaatc gaaatcaaag ccttgatcga attaggtcga cgaattcaga cggatcatga
    18721 aagagcggct ccaccggttc gtaccagcta cgaattggcc cagcaattga ttctggaaat
    18781 gaaagatcac aaacaagaac atttggtttg tatttatttg gacacgaaga atcaagtgat
    18841 cttaaaaaaa acagtgttca tcggttcgct gaatcagtca gtcgctcatc ctagagaaat
    18901 tttccattat gctgtacgtt attgtgcagc aaggatcgtt ttagcgcata atcatccttc
    18961 tggtaatgtt ttaaccatag tataataaat aatagtagtt aaagtagttt aagaggagat
    19021 attaaaggga tattttactt taaataaaaa ttaacaaggt ttataatttg tagtagttaa
    19081 agtatttaaa gaggtgggaa catgtgaagg ttcaaaggat agaagtggag aataagccgt
    19141 atccattgta tttattacta gataaagaat accagctaat agaaccagta atgaaattta
    19201 ttaaatactt agataatact ggtaagtctc ctaataccat taaagcatac tgctatcatt
    19261 taaagttgct gtacgagttc atggaacaga gaggtgttat tcttaatgat attaactttg
    19321 agttgttagc agacttcgta ggttggttga gatatccttc agcatcaaat gtaattgatc
    19381 ttcagtcaaa aaaagccata agagaagaaa cgacagtgaa tacaatttta aatgtagtta
    19441 tgagttttct tgattattta agtagattag gagaatttaa atcaattgat gtatttaaac
    19501 aagcaaaggg aagaaatttc aaaggatttt tacatcatgt taataagggt agataccaaa
    19561 agaatgtctt aaagttaagg gttaaaaaga aacagataag aacattgaga tcagaggaag
    19621 ttaagcaaat tattgatgct tgtcatacga aaagagataa attaatttta atgcttatgt
    19681 atgagggtgg tttaagaatc ggtgaagtgt tatcgcttag gcttgaagat attgtcactt
    19741 gggacaatca aatccattta acacctagag atgttaatgt taatgaagct tatattaaat
    19801 taaggaagga aagaacaata catgtgagta aagaacttat gtcactttat acagattact
    19861 tgatatatga gtatagtgag gaattggagc atgattatgt ttttatttcc ttaaaagaag
    19921 gctattttgg gaaaccacta aagtaccaaa gtgttcttga tctagttaga agaatagtta
    19981 aaaggactgg aatagaattt acatcacata tgcttcgcca cactcacgca acgcagctaa
    20041 ttagggaagg atgggatgtt gcgttcgttc aaaagagatt aggtcacgca catgttcaga
    20101 caacgttaaa tacctatgtt catctttcag atcaggatat gaaaaatgag tttaataaat
    20161 acctcgagag aaaggagcat aagaaatgaa tgcttctagt aaaaggaaaa ttattagtca
    20221 gagtgagatt agcaaaaaaa tagctgtaat gaatgaagaa atgcaggggt tttgggctaa
    20281 taatagttgg gatataagaa aatgtccaca tccttctgcc atagaattaa gtaagaatcc
    20341 tgctttaagg aatcgttggg ttcgttttga acgtgttaaa aatctgtggt taagaacaga
    20401 attgaaatat ttttattttt accatttaaa caatggaata tggaatgcaa aaactgtctg
    20461 gattagaaaa ggaacagtaa ttaataaaat gttagatttc ttagatttaa agtatcctag
    20521 cattacttca attactgaag ttcctattga aaaagcaatg acggagtata gaacttattt
    20581 gacaaaacgg ggtgttagaa ttaccactac taattataag attactgcta atcaagaaaa
    20641 aacacctgta aaagctaatt cctactatgt tactaatcta aaacaattta tggagtttta
    20701 tgagaacttt tattttgatg gagaggagtg ggataaagac gtttgggata gacgtaactt
    20761 acctttgcca gatgataagg ttaacccaac acaatatgaa tatacaatta actttaaagg
    20821 gtttcggaat acatatttta aacaacttgt aaaaagatat tgtaagttga gattgaacat
    20881 ggatagcttt tcctatgtaa gtgatattgc ccaaagactt aaagagttct ttaattttct
    20941 ggacatgaaa tttaaacaag ttcagagagt acaccaatta acgagagtgg aaattgaagc
    21001 atatttaagt gaactaaaca tgatgggaat aaaacctagt acaataactg ggaggatctc
    21061 tatattggaa ggactattta gtacccttct taggctagaa tgggatgatg ttccttccaa
    21121 aatattaatt tattctgagg actatccgaa aataccaaga gcaaaaccac gctttataga
    21181 tgaattcgtc ctagagcaat tgaacagtca tcttgataaa ttacccgaat atatagctac
    21241 gatgactatg attgttcaag aatgtggaat gaggataagt gaattgtgca ccttgaaaaa
    21301 aggctgtcta ttagaggaca aagatggaga tttcttttta aagtattatc aatggaaaat
    21361 gaaaaaggag catatagttc caatatctaa agaggtagct ttacttatta aagttcggga
    21421 agataaagtt tcagaggaat ttccagatag tgaatacctc tttccaagaa aagatggatc
    21481 gccattaaaa caagaaacat ttagaggtga gttaaataaa ttagcttatg agcaaaatat
    21541 agtggataaa tcaggtgaga tttatagatt ccatgcccat gcctttcgcc atacagtagg
    21601 aacaagaatg attaacaacg ggatgcccca gcatattgtg cagaaatttt tggggcatga
    21661 aagcccagaa atgacaagca gatacgctca tatctttgat gaaactctaa aaaatgaatt
    21721 tactaaattt caggaaaaac tggttaccaa taatggagat gtgcttgatc tagatgaaga
    21781 taatgaagtc gatgatgtag agcttcaatg gttcaagaaa aatataaatg cacaagtgct
    21841 tccaaatggt tattgtagat tgccagtagt agcaggtggt tgtccacatg cgaatgcatg
    21901 cttagattgc actcacttct gtaccagtaa gcaattctta ccacagcacg aagaacagtt
    21961 agagcgtaca gaagagttat tagccatagc taaggataaa caatggcaaa gacaagtaga
    22021 gactaatagc cgtgttaaag agcgtttaga acaaatcatt ggaagtttga cggggtaatt
    22081 atcaatggat aaacaagtta gaaatacaac agaaattgta cgtttggcga agcagaaatc
    22141 aaaaaagaca agggaaaaag tagacaaagc gatttctaaa ttttcgattg aaggtaaagt
    22201 tattaatttt aattcaatag caaaggaagc taatgtttct aaatcatggc tttataagga
    22261 acacgatatt aggcaaagaa tcgaatccct tcgtgagcgt caaataacag caaatgtagt
    22321 ctcaaaaccc aagaaaagtt ctcgttcgga ggaaatcctt attaaaacct taaaaagaag
    22381 agtaatggaa ttagaaaaag aaaataaaaa attacagaac caaattcaaa aattatatgg
    22441 agatctgtat aataaagaat aattattaat ctgtagacaa attgtgaaag gatgtactta
    22501 aacgctaacg gtcagcttta ttgaacagtc atttaggtat atgtccaatc tagggtaagt
    22561 aaattgagta tcaatataaa ctttatatga acataatcag ttacgaggtg aaatcatgag
    22621 caatttgatt aacggaaaaa taccaaatca agcgattcaa acattaaaaa tcgtaaaaga
    22681 tttatttgga agttcaatag ttggagtata tctatttggt tcagcagtaa atggtggttt
    22741 acgcattaac agcgatgtag atgttctagt cgtcgtgaat catagtttac ctcaattaac
    22801 tcgaaaaaaa ctaacagaaa gactaatgac tatatcagga aagattggaa atacggattc
    22861 tgttagacca cttgaagtta cggttataaa taggagtgaa gttgtccctt ggcaatatcc
    22921 tccaaaaaga gaatttatat acggtgagtg gctcaggggt gaatttgaga atggacaaat
    22981 tcaggaacca agctatgatc ctgatttggc tattgtttta gcacaagcaa gaaagaatag
    23041 tatttctcta tttggtcctg attcttcaag tatacttgtc tccgtacctt tgacagatat
    23101 tcgaagagca attaaggatt ctttgccaga actaattgag gggataaaag gtgatgagcg
    23161 taatgtaatt ttaaccctag ctcgaatgtg gcaaacagtg actactggtg aaattacctc
    23221 gaaagatgtc gctgcagaat gggctatacc tcttttacct aaagagcatg taactttact
    23281 ggatatagct agaaaaggct atcggggaga gtgtgatgat aagtgggaag gactatattc
    23341 aaaggtgaaa gcactcgtta agtatatgaa aaattctata gaaacttctc tcaattaggc
    23401 taattttatt gcaataacag gtgcttactt ttaaaactac tgatttattg ataaatattg
    23461 aacaattttt gggaagaata aagcgtcctc ttgtgaaatt agagaacgct ttattacttt
    23521 aatttagtga aacaatttgt aactattgaa aatagaaaga aattgttcct tcgatagttt
    23581 attaatatta gtgacatttg catgcttcaa agcctgtcgg aattggtttt tagtgaaaag
    23641 aacacgatat tcacggttta cccacttata aacaaaagat cgatactttt tgtagtcctt
    23701 ctttgaaatc aatggttgat gtcgttcaag aacaatcaat acagagtcta cacttggctt
    23761 aggatgaaaa tatagtggtg gtactttttt gagcattttt atatccatct ccaccattaa
    23821 tagtaaaccc aaagctcgtt gcagattttg caatcttttc gcaaatccct tctcaacgat
    23881 aagatagcta tatttagcct gactttcaaa ggtaattctt ttgacaatat ccgtactgat
    23941 gttataagga atattaccat atatcttata gtttatatgt tttgggaagg aaaattttag
    24001 aatatccgtt tgaatcactt ttatattctc agaggggttt accgcttctt tagtcacttg
    24061 acataagcct ccatcaattt ctatagcagt aactgatcga ctcattttga ctagctcttt
    24121 ggtaaaatgt ccttttcctg atccgatttc tattacgttg tcttgtttac tgatattcgt
    24181 gtgattcaat atttctttta catgcttttt agaagtaata aaattttgcg tgtctttagg
    24241 gtttttctgg ttcattataa ccttctcctt actggttata atgaactgac attaagcgtt
    24301 cattataacc aattaatttt gatttggttg ataacgaact ttattaataa caaatataga
    24361 aaa
//
LOCUS       DACDTO010000036        23831 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDTO010000036 DACDTO010000000
VERSION     DACDTO010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.036"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(55..789)
                     /locus_tag="IWK70_001463"
     CDS             complement(55..789)
                     /locus_tag="IWK70_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9613996.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1116..1526
                     /locus_tag="IWK70_001464"
     CDS             1116..1526
                     /locus_tag="IWK70_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9613997.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1519..2220
                     /locus_tag="IWK70_001465"
     CDS             1519..2220
                     /locus_tag="IWK70_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9613998.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2220..3806
                     /locus_tag="IWK70_001466"
     CDS             2220..3806
                     /locus_tag="IWK70_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9613999.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3825..4046
                     /locus_tag="IWK70_001467"
     CDS             3825..4046
                     /locus_tag="IWK70_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9614000.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4043..4804
                     /locus_tag="IWK70_001468"
     CDS             4043..4804
                     /locus_tag="IWK70_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9614001.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5184..5693
                     /locus_tag="IWK70_001469"
     CDS             5184..5693
                     /locus_tag="IWK70_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9614002.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5768..6307)
                     /locus_tag="IWK70_001470"
     CDS             complement(5768..6307)
                     /locus_tag="IWK70_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9614003.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWK70_001471"
     CDS             complement(6447..7058)
                     /gene="rpsD"
                     /locus_tag="IWK70_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9614004.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7374..8171
                     /locus_tag="IWK70_001472"
     CDS             7374..8171
                     /locus_tag="IWK70_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9614005.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8199..8834
                     /locus_tag="IWK70_001473"
     CDS             8199..8834
                     /locus_tag="IWK70_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9614006.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8854..9627
                     /locus_tag="IWK70_001474"
     CDS             8854..9627
                     /locus_tag="IWK70_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9614007.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10035..10832
                     /locus_tag="IWK70_001475"
     CDS             10035..10832
                     /locus_tag="IWK70_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9614008.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10810..11223
                     /locus_tag="IWK70_001476"
     CDS             10810..11223
                     /locus_tag="IWK70_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614009.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11207..13852
                     /gene="mutS"
                     /locus_tag="IWK70_001477"
     CDS             11207..13852
                     /gene="mutS"
                     /locus_tag="IWK70_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9614010.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13870..15978
                     /gene="mutL"
                     /locus_tag="IWK70_001478"
     CDS             13870..15978
                     /gene="mutL"
                     /locus_tag="IWK70_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9614011.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            16000..16560
                     /locus_tag="IWK70_001479"
     CDS             16000..16560
                     /locus_tag="IWK70_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9614012.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWK70_001480"
     CDS             complement(16645..18633)
                     /gene="treP"
                     /locus_tag="IWK70_001480"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9614013.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18856..19569
                     /gene="treR"
                     /locus_tag="IWK70_001481"
     CDS             18856..19569
                     /gene="treR"
                     /locus_tag="IWK70_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9614014.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19646..20092
                     /gene="msrB"
                     /locus_tag="IWK70_001482"
     CDS             19646..20092
                     /gene="msrB"
                     /locus_tag="IWK70_001482"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9614015.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20262..20864
                     /gene="ruvA"
                     /locus_tag="IWK70_001483"
     CDS             20262..20864
                     /gene="ruvA"
                     /locus_tag="IWK70_001483"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9614016.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20877..21878
                     /gene="ruvB"
                     /locus_tag="IWK70_001484"
     CDS             20877..21878
                     /gene="ruvB"
                     /locus_tag="IWK70_001484"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9614017.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21907..22485
                     /locus_tag="IWK70_001485"
     CDS             21907..22485
                     /locus_tag="IWK70_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9614018.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22537..23019
                     /locus_tag="IWK70_001486"
     CDS             22537..23019
                     /locus_tag="IWK70_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9614019.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23136..23510
                     /locus_tag="IWK70_001487"
     CDS             23136..23510
                     /locus_tag="IWK70_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614020.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7783 a         4285 c         5114 g         6649 t
ORIGIN      
        1 ataaaaaaac ttggtacgca atcgctgtac caagtttttt tatttagctt ttgattaagc
       61 cataaataat aatttatata agaatactgc tacgacacca gcacagatag gtgctacaac
      121 tggtacccat gcatatcccc attgagaaga acctttatgt ttcaatggaa gaagtgcgtg
      181 tacaagacgt ggagcaaagt cgcgggctgg gtttagtcca ggacctgtag gaccaccaag
      241 tgaagcaact aatgtccata caaggaaacc aagtgctaag tgagctgtac caagattatc
      301 ttggaagaat ggtgcttttg taatacctag ggcaccaaag aataaaacga atgaaccaaa
      361 gaattcattg acgaaaccat tgaatttaga accaacgctg ttgattgttg agaatgtacc
      421 taacacatgg tttacgtctt ctgtttggtc gtagtaaggt ttgtaacaag ctactacgat
      481 caattgtcca atcgctgcac caatcaattg tgcaatgata taaggaacaa cttcagccca
      541 agggaataga ccattagatg ctaaagcaat tgtaaatgct gggttgatgt ggttaccgct
      601 gattccacca aaaatcattg ctggcatcat aaccccgaat ccatacccga cagcaatcag
      661 catccagtca ctgccatatc cttttgtacc ttttaaatct acgtttgcta cagcaccgtt
      721 acctagaacg attagtaatg ctgtaccaat catttccgcc gcaagacggg tcattagtga
      781 gtaatccata aatacctcca aaagttataa aaatgcaaaa aaaaaagaaa catcaacacg
      841 aaaagcatcg gtgcagtgtt tcgtgtcgga aggatttctg cagtcttaac aaatgtgaat
      901 aaaaaaatga attccattac ttataagaaa ccagaccaac agtaaaactc caactctttt
      961 taaaagttct ccacaaatca ccaagcttaa ctataaatat accaaaaaaa aaacgttttt
     1021 tttctcaaaa attgtatctt agaaacagca tgttttgaaa ctttcagaaa aggtttgtta
     1081 gtaaaatgaa agctatacgt aaggagtgtt gtttgatgaa attttttcaa gagtttttca
     1141 ttattatttt cttttctgta ttaggagaag gcatacggat ttttgctcat ttacctattc
     1201 ctggaagtat cttaggtatt ctttttttat ttttagcatt tgaaatgcat cttgttgatc
     1261 ctgaaaaaat cggagcaact ggtgattttt tattgaataa tctagccatt ttatttgttc
     1321 ctgctggtgt aggtttattg gaatattttg atgatattgc ttcgatatgg cctgttttac
     1381 tgggagcagt cgtcgtgtgt tccatcgtca cgatggttgc agcgggaaaa acggcagaag
     1441 gtgtagaagc tttgctgggc tatgtccgta agaaaaaagc agaaagggta tctgtaaacg
     1501 aaatggagga gaatcaaatt gactgagttt tttgtgaatc cattatttgg cttaagtttg
     1561 actttaggag tgtacgtatt atctgacaaa tttttgcgga aatataaact gcctgttttg
     1621 aatcctctcg tattatcgat tgttgttatt atcttatttt tgcatttttc tgggatcagc
     1681 tataaagact ataatatcgg gggcaatttt ttgactatgc tgattactcc tgcgacagtg
     1741 gcacttgcca tccctcttta taaaaacttc cgtttactga aagagaattt ttatcctgta
     1801 atggctgcga ttttagcagg aatagtcgca aattgcttgg ttagtgtagg gttcggttac
     1861 ttcttctctc ttcataaaga gatggtgatc tcattactac ctaaatctgt aacgacggct
     1921 atttctgtcg atttatctca tacgatgggc ggggtgaatg ccgtgacact tgctattgtt
     1981 gtctctacag gtatttttgg ttcgctgatc gccactcata ttttccgcct attcaagatc
     2041 gaaagtccag ttgctcgcgg tgtggcgtta ggaagtactt ctcatgctat tggtacagca
     2101 aaagcaatcg agattggtga aatagaagga atcatttccg gacttgcgat atgcgtcaac
     2161 ggaatattga ccgttttatt gctaccgtta ttctttcagc cttttgctgg cttgttttga
     2221 tgcgaaaacc actattttat gctacaataa ccaaaacgtt cgagcataaa aaaggaggga
     2281 aagacgtgta tttgtcttcc gagtatctta aacaatttga taaagatcta tattataaga
     2341 ttctcctcct agaaagtttt gaggatcaag cttggcatac cgctgcacaa ttagcgcaag
     2401 ttgttcagct ggatgcccgc agcgtgtcga aatacctgaa cgagctgtct aaaaactatc
     2461 agcagtttag cggtaagaca caccctttgt ttacaaaaaa tcaccgttct ggttataact
     2521 tttatgatac gctcgattca attgaacatg agcgtttttt gatctatctc gtgcaaagta
     2581 cgctgaaatt ccagttgctg cacgacattt tttttgaaga attccataca atgtaccaat
     2641 ttgcgcaaaa gcattacatt agtgaatcta ctgctcatcg aaaaatcaat gaatggaaac
     2701 agcaactgca aacttacgga attcggctgc aacgaggtac atatatcgct caaggcgaag
     2761 aagaaatcat tcgactttac ttgcatatga cattctggca gttgtttcga gggaaaatct
     2821 ggccatttga aacgatttct caaatggacg taaagaatat ggcagaacat atcatggctt
     2881 tttttaatgt tcgtctgaac gagatcaaaa aaagacgatt ggaatatatg ctaggtgcct
     2941 tttttttaag aaaatcacag aagcattatg ttgtattgaa tgagaaaaaa agacggctga
     3001 tttctgacaa tctgttgttc cagcgatttt gccaggtcat ggagccggta tttcctaatt
     3061 attttcaagt agaagacgag ttaggcgcat tatttcttgt actgatgact cgtgaagaat
     3121 attatagtga tcctaagata cggaagaaaa tttttgattt tcatcaacaa gcaaaaacac
     3181 cgccctttac agccctttca gaagcaaaag ccgctctttc tctttatcag gaagagcagg
     3241 ggctgccagc agaaaatcta accttcgagg cagaaaacta tttattcagc agtcattttt
     3301 tcgcctatct ttttcctaat gcgaaagaaa cgattgatgg caatagtagt gattttatta
     3361 atcatttagt tattgaaaac aaggaattga aacaatggct cgtacatttt tttgaaagta
     3421 gacacaaaca tcccaatcat ctggcattta aaaatcatgc gtttttgatg gggcggtatc
     3481 tgactgtgtt taaaacatta ggagccttta ctccacagct tccaaagatc acgatccttt
     3541 taatgacaga tttcccatta tttgaagagc aactgttaga ggagggatta cggacatttt
     3601 tccgtaatga gtatcagctg atttttctgc caacagatta tcgggggaga gaagttgatc
     3661 ttctgatttc gacaagtaaa gtccaccgca aaccatgggc ggatcttgac tattttattg
     3721 tgactgaaga actaaaatta attgattaca tccaactatc tcaaaaattt gaaatgattc
     3781 aaaaacaaaa actattaaaa caataacaaa gaaggagaaa aaagatgaca tttgaagaaa
     3841 ttttaccaga attgaaaaaa ggtgcaaaga ttattcgtaa aggatggagt ggatttgaac
     3901 tttatgttgt tctcgtatcc gatgatgaat ttgatggctg tccggtgacg ccgtacttat
     3961 tgatcaaaac aagtgacgaa ggtttttcca gcttcgctcc tacagtttgt gatattttag
     4021 cagatgattg ggcgatcgta gaatgaaaaa ccagtatcca gatctagcta aacaggttgt
     4081 atttatcaca ggagctgctt ctggaattgg tgctgcgcaa gcccgagcgt ttcttgaaca
     4141 agatgctttt gtatttggat tagatcaaaa gttcggtaaa atggaagaaa tttctttgcg
     4201 atttcctgaa acttttgcct acgcactagg tgatgttcga caaatgaaag aactccaaca
     4261 agcggtatcg aaatgccaaa atcattttgg tgaagttacg attttactga atacagcggg
     4321 gatattggat gcctacaaac ccttgatgga aactgacgaa tcgctgtggg atctgattta
     4381 tgagacgaat gtgaaaagca tgtatcagct gacaaaaatc atccttcctg atatgatccg
     4441 ccaaaaaagc ggaacgatca tcaatatggc ttcgattgct ggcttgattg ctggtggagg
     4501 tgggatcgct tatacaagcg cgaaacatgc gattgtcgga tttaccaaac aactcgctct
     4561 ggattacgca ggacagggta tccgagtaaa aggaattgct ccaggagcca tccaaacacc
     4621 aatgaatgcg gctgattttg aagggaaagg tgagatggca gagtgggtgg caaatgaaac
     4681 accagtcaag cgatgggcac agccggaaga agtagctgag ctgacgttat ttcttgcctc
     4741 tcctcaagca agctacatac aaggaacgat catcccaatc gacggcggat ggttgttaaa
     4801 ataaccttgt gtttttttca agcttctgta tgataaaact aaagagagaa acaacggttg
     4861 atgataaaaa aagtcgatga ctgttcccga cattcgatgc cgaacagcca gttcaaaaaa
     4921 ttctttttaa taatcgttaa tattcgaaac taagcggctt ttcacgacct cttgatataa
     4981 acgatattca aaagcttgca actgcggtaa caagtccagc caaatgaaaa atataaggag
     5041 ctatttttat ttgaccattc ttattacttg ttgcagggca gctttttcac taccttctgc
     5101 gacacttcag ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt
     5161 atcttgcaaa ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga
     5221 caacgacaga tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg
     5281 ttttgtctgt ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc
     5341 tgtccttgta taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata
     5401 ttgcttctcc tcttggactg atagatgttg cagtaggaag cgtcagcacg ctgcttgtat
     5461 tgcttatcaa ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atggccgtga
     5521 cagcatgcgt atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc
     5581 aactaccttt tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg
     5641 tcggtgggat aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg
     5701 tttaatttaa aatagggcag catggaagag actgaatgtt gagtcttctt ccatgctgct
     5761 tttttgatta atttgtatga agaaatcgat ttagccgttt tttcatcaag ataccgatat
     5821 aagctgctcc agttgccttg attgcttccg gcagtaaaaa cggcagtagt ccactggcaa
     5881 atgcagttgt aaaagtaaga tcaccactta ttttcagcca gatcgttccg cagatcaatg
     5941 cagcaagaga gcccattaga ttggcaaaga ttgcatataa gtaatgagca tccgttcttt
     6001 cgatcatcca accagttatc cacgtttgta agagaaatcc aatcaaatat ccgccagttg
     6061 gcccaaataa aacagctata cctccagtca tccctgaaaa tacaggaaga ccaatcaagc
     6121 caagtaaaag ataaactcca acagatatca atcctagttt tcttccgaga aaagttgctg
     6181 ttaatccaat agcaaatgtt tgccctgtca aaggaaccaa accaaaagga atggtcatct
     6241 gagaaaggac agaaataatc gcggcaaatt ccgcagcaag tatcaattct ttcaatgttc
     6301 ggttcataac gccctctttt cgttaactta atatttttat ttggttaacg aaattgtaaa
     6361 acaaagtgat tactttgtaa atagaaaata taaaaaaaga ccagctatcc aatagatagc
     6421 ggtctttttc aagaaagctt ttgaaattat aagctttggt tgtagtattc aacgacaaga
     6481 gcttcgtcga tttctggata tagttcgtca cgttctggta aacgagtcaa tgaaccttcc
     6541 aatttttcag cgtcaaagct tacgaatgct gggcgtccaa cagttgcttc tacagcttct
     6601 tttactgtta cgatgttttg tgatttttca cgaactgaga tcacttgacc cacttctacg
     6661 tggtatgaag ggatatcaac gcgtttgcca tcaactaaaa tgtgaccatg gttaactaat
     6721 tgacgtgctt gacgacgagt cgttgcaagt cctaaacggt aaacaacatt gtctaaacgt
     6781 tgttctagta agatcatgaa gttaacacca tgtttacctt ctttgatttt gctagctttt
     6841 acgaacaagt tacggaattg acgttcgttc attccgtaca tgtgacgaag tttttgtttt
     6901 tcagttaatt gcatgccgta ttctgattgt ttgccacggc tattaggtcc atgttgacct
     6961 ggtgcgtaag ggcgacgtgc taattctttt cctgtgcctg acaatgagat gcctaagcga
     7021 cgagaaattt tccatgatgg tcctgtataa cgagacatta aaatttcctc caatgaatat
     7081 aagtttttgg agtaaaataa tccgttgaaa aattcataat catatagttt gttcttcaat
     7141 cttcaccttt gcagccgcgg ttacacaatc gaacccgaga cggggcaaca aactagggat
     7201 gagttattat ttttctgctg cattatttta cacaaattac agtatatgat agatcatcca
     7261 cgaatgtcaa gataattagt acataaaaac gctttacaaa cctaggctgt ctttataaaa
     7321 tgagagggta tgtgcaaatg gaaaaaagcg aaggaagttt aaggagaaaa tcaatgaatc
     7381 cggtatcacc attatttgag aaaatcgatg cttcaattga aaagaaggcg aatctttttg
     7441 aaaacagctt ttcacgttac gctgttcgag cggttctcgc ctgtttgttt ttgactttag
     7501 gaacggctgt ggcatttgcg atcgcgatga agggggaaga tatcgcgcat ggtctaggaa
     7561 agattttata cgcatttatg ttcagctggg gtctggtcat gattctatac atgaacgccg
     7621 aattaggaac atcgaatatg ctttatatga ccgtgggtgt ttttcggcag agacttgctt
     7681 taagtaaggc agccaagatt ctatttgcct gtattttttt caatatgatt ggcggtatat
     7741 tatgtggatt tctaatttct atgactacgc catttcaaga tttaacgttg gacaacttta
     7801 tgttagaatc aatagcagta aaactagcaa aaccttcagc aacgattttg gtggaaggga
     7861 tgtttgcgaa tatcgttgtc aataccgctg tactgatcag catgcggatg aaagaggatg
     7921 tcggaaaagt gatcacagtc gtattcatca ttttcatttt tgctttttta ggatatgaac
     7981 acgtgatagc gaattttcct gcctttacgc ttacttactt tgcttctcac ggacagatgg
     8041 atggaatgac agtcagcaat gtgttacata atttgttttt tgctttggca ggaaattata
     8101 ttggtggcgg tttagtaatg ggactgggat atgcttggct gaatcagtca aaatcatcct
     8161 atgtggacta aaaagtcgtt cattaaaggg gaagtgccat ggaagtttta gtttttccaa
     8221 aaaatgagga aaaagcgtta caaatggaaa agtacatgaa aaatcttttt tcctttgctg
     8281 gagtacctaa gccggaacgc gctcttttac aacaaccgtt actggatgag agcaaaaatt
     8341 tatctatttc tgaagtttta gcttttgttc attattatta tgaacaaatc gaaagagagt
     8401 atcagtatgt agcgatcgat ttagtccaga aaaacatcaa acggcttaca tttgaagact
     8461 tagtcgtatt gttgccacta gttcagaaaa aagaatggtg ggacagcatc gactcttgga
     8521 gaaaagtata cagcacatgg tgtaaagcct acccagagca gcgtgaagaa gtctttcaat
     8581 tgtttgaaaa aaaggaagat ttttggctac gtcgaatagc aatcacgttg caattaggtt
     8641 ataaacagca aacagacaca ggactattga tccgagcaat agacgaagat cgtcaaacaa
     8701 aggaattctt tatccaaaaa gcaattggtt gggcattacg cgattatagt aagaccgatg
     8761 ccgattgggt aaaaagacag ctgacaacag atttgagtaa gttagctgtt cgagaggcaa
     8821 gtaagtattt ataagaaagc gagacgacaa gagatgagac gcaaattatt tgcttttgat
     8881 attgatggaa cgttgttaga ttcaaacaaa caagctttag atagcacaag agaagcattg
     8941 gaaaaattac gcaaagccgg tcatctggtc acgattgcta cagggcggag ccgatttcat
     9001 gcgcaagaga tcattcgcga tcttgatttt acgaactaca ttttatgtaa tggagcggcc
     9061 ggttttttag accatgaaca agtatataag aatttactag atcgcgacca gttggatgcg
     9121 ttcgttcaag aagcgcatca tgagcagatc gatacagctt ttgtcagctt agacaatatc
     9181 aagcgttctt cttccaatga tgtaacggtg atggaagaag cgatgcattc tttcggtgct
     9241 gtcttgcctg aattagatca gttttttgca gaggaacaag aaatatacca agcgttagca
     9301 tttttcgatc aaacatatga tgggaagttc tcttcatatg ataaaattcg atttgtccgc
     9361 tggcatgaga acagtgtaga tgtcgtacct aaaaatggtt ctaaagcagc gacaattctg
     9421 cacttagcag aacaagtagg catccctcat gaagacatca tcagctttgg cgatggacaa
     9481 aacgaccgtg aaatgttgcg catgtcagga attgggatag ctatggggaa tgctgtgcca
     9541 gaagtacaag cagaagctaa gatggtcact gatacaaacg atcaggatgg aatttggaag
     9601 gcattgaaag agttaagcgt gatttaaaag aggttgtgaa aaagctgttc tgcatcaagt
     9661 aataagccca gccaaacaaa aatagctcct cctatttttc gtttttctgg gcttattacc
     9721 gcagatgcaa gctttcgaac accgtttaag gaagaggtcg tgacagaagt ggtcagtttc
     9781 aagaaataag aagggattta cgaaaattgc ttctcaaatt tttgtaaaat tacggcttat
     9841 ttctgaagaa gtagatgaaa caacgtttat tcgggaaatg ggactgtgac aagatgtttg
     9901 tcatagtccc atttttttat tttatttttc aaaactaaga aataccagga aagagttcct
     9961 tttgtttgac cttttctttc ttattgaaaa caactataat atagagtgat gttaaaagag
    10021 aagaggaatt tagtgtgcgg gtattgttta ttggtgatgt agtaggttca atgggacgag
    10081 agatgattac tgaatatctt cctagattga agaagaagta ccgtccgcaa gtaacgattg
    10141 taaatggtga gaatgccgct tctggacgtg gaatcacaga aaaaatctat aaaaaatttt
    10201 tgcaagacgg cgtagatgtc gtgacgatgg ggaatcatac gtgggacaac cgcggaatct
    10261 ttgagtttgt aaatgaagct aaaaagatgg ttcgtccggc aaattttccg gaagggacgc
    10321 ctggacaagg gatggtcttt gtcaaagtga accaagtcga actggcagtg atcaacatgc
    10381 aggctcgttc ttttatggtg gacttggatg atccttttcg taaaatgaat gagctggtag
    10441 aagaagccag aaaacgtacg ccgatcattt ttgtagattt tcatggagaa acaacaagtg
    10501 aaaaacaagc aatgggctgg tttttagatg ggaaagtttc agcagtggtc ggtacacata
    10561 cacacgttca gacaaatgat gcgcgaattt tgccaagagg aacagcgtat ctgacagacg
    10621 ttgggatgac aggtccctat gatggtattt tgggtatgcg cagagaacct gtaatcgaaa
    10681 agtttctgac tgcattacca aaacgttttg aagttgtgga acaaggccga tcgattttaa
    10741 gcggctgtat cttggaattg gatgatacga caggtcatgc aaaagaaatc cagctgattc
    10801 agatcaacga tgatcgaccg ttcatggagt agaaagagag gcagtttttt gcagcaggag
    10861 ccggaaattc aaaaagaagt aaggaaactg acaaaacttt taagagagaa tgaaacgatc
    10921 attcgctaca aagaacttga agaaaagatc cagcaaaacc agtatttagc tgagttgaga
    10981 gaaaaaatca aacaagcgca aaaagatgct gttcattttg cccactatga caaaccagca
    11041 gcagaaaaag aagcaatcaa acaagcggat caattcatgc aagaatttga tcagcatccg
    11101 cttgttgtag cttatagaaa acagctgttg gaagcagatg atctgcttca tcatttaaca
    11161 actatgatac aagaagaaat caatggacaa atagaggagg aaaaacatgc ctcaaaaaac
    11221 taaaaatact cctatgatgg aacaatatct aagcatcaag gcccaatacc aagatgcttt
    11281 tttgttctat cgtttaggag acttttatga actattttac gaagatgcga tcaaagcgtc
    11341 acagcttttg gaattaacgt tgacgagcag aaatcgaaat gcagaagaac ctattcctat
    11401 gtgcggtgta cctcaccatg cagcacaagg atatatcgat gccttgattg aaaaaggcta
    11461 taaagtagcg atttgcgagc aggtagaaga tccaaagacg actaaaggga tggtcaagcg
    11521 cgaagtgatc cagctagtca ctcctggtac agtcatggac agcaaaggat tgtcggcaaa
    11581 agacaacaat tttttaacag ctgtagttag tcaaggaaat gaatttggct ttgcttatgc
    11641 cgatttaagt acgggagaat tgaagacggc tcggttgcat gatgaagaag ctgttttaaa
    11701 tgaagcctct gcattgcaga caaaagaact cgtattaggc agtgagatca ctgataccct
    11761 tcgtgaacag ctaagcgggc gtttagggct agtcttttct caacaaaatg agatggaaga
    11821 aaacgcggaa tttagctttt tgacaagcga actagtcgat ccgctggaaa aagaagcaac
    11881 cggcaaacta ttgacttatt tatcggtgac acaaaagcgg agtttagctc atatccaaaa
    11941 agcagtagaa taccaaccag atcatttttt gaaaatggat tattattcaa aattcaacct
    12001 agaattaagc caatctattc ggactgggaa aaagcacggc actcttctat ggttgttaga
    12061 tgaaacaaaa acagcaatgg gtggccgttt actaaaacaa tggctagacc gtccgcttat
    12121 ccaggcaaaa caaattaaag caagacaaga aatggtcgct tctttgctag acagctattt
    12181 tgaacgtatc gatttgcagt cagcattaac gaaagtctat gatctggaac gcctagcagg
    12241 tcgagtagct ttcggtaatg tcaatgggcg agacttgatc cagctgaaaa cttctttaga
    12301 acaagtgcca atgatcagag ggttgattga aggaatcgat caaggtcaat ggcaaagtct
    12361 gctttcagaa atgcagccga tggaccatct cgtccaattg atcgaccaag ctatccaaga
    12421 tgatccgccg ttacaaataa cggaaggaaa catcatcaaa gatggattca atgagcagct
    12481 cgatatgtac cgttctgcta tgagaaacgg aaagaaatgg ttggcagaac ttgaagcaaa
    12541 agagcgtcaa gaaacaggaa tcaagaactt aaaaatcgga tttaaccgcg ttttcggtta
    12601 ttatatagaa attacaaaag caaatcttgg aaatgcagaa ctggagaagt atgaacgaaa
    12661 acaaacttta gcaaatgcag agcgtttcat cacaccagaa ctaaaagaat tggagacaca
    12721 aatcctcgaa gcagaagaaa aatctgttga tttagaatat cagttgtttt tagctgtgag
    12781 agaagaagtc aaaaaggcta tccagccgct acaagtactt gcaaaagcaa tcagtgcagt
    12841 agatgtgcta caaagttttg caacgatcag cgaacgctat cagtatgttc ggccagaact
    12901 tgtcagtgac aagcatcagc ttctgatcaa agacggacga catcctgtag tagaaaaagt
    12961 attaggacat caggaatata tccctaacag cgtagaaatg gcagaagacg aaatgatctt
    13021 gctgattaca ggaccgaata tgtctggtaa aagtacgtat atgcgacagt tggcattaac
    13081 tgttctgatg gcgcaaatgg gctgtttcgt tccggcaaaa caagcagttt tacctatttt
    13141 tgatcggatt tttacccgaa ttggtgcaag tgacgactta attgctggac aaagtacttt
    13201 tatggtagag atgatggaag ccaatcaagc cctgcgttat gctacgccaa acagtttgat
    13261 tttgtttgac gaactagggc gaggaacagc aacctatgac ggaatggcgt tggcccaagc
    13321 aatcatcgaa tatatccatc gtcatgtaca agcaaaaaca ctcttttcta ctcattatca
    13381 tgaactgacg gtacttgaga aagaacttcc gcagcttaaa aatgtccatg ttggagccgt
    13441 tgaaaaagac ggagaagttg tcttcttgca taaaatgatg gatggacctg cggataaaag
    13501 ctacggcata catgtggcaa agatcgcagg attgccttcc aatctactag aacgagcagc
    13561 agatatcctg catacgctag aaaatggcga taacggacac catactgttc cagctgaagt
    13621 agaagagaaa actaaaacag ccaaacagga gcaaataaaa ccacctgcag aaaaagtaaa
    13681 tcaagaccat atacaagacc gtgtaaaaga agaaaaccaa cagctttctt tattcagtga
    13741 attatccgaa aatgaaacag aagtcattga atcactgaag caaataaatt tactagaaat
    13801 gacgccaatg gatgcactaa acacattata tgaattacaa aaacaactgt aggaagaagg
    13861 cggatatcga tggccaaaat ccaagaatta tctgaacgtt tagctaatca gatcgctgcg
    13921 ggagaagtag tggagcgacc agcttccgtt gtgaaagaat tagtggaaaa tgcaattgac
    13981 gccggcagta ctcagattga tatcttagtt gaagaagcgg gactgcggac gatccaagtg
    14041 attgataacg gagaaggcat tttggcagac gacgtagaga atgccttcaa gcggcatgcg
    14101 acaagtaaga tccataatcg cgacgatctt tttcgcatac gaacgctggg ctttcgaggg
    14161 gaagctttgc caagtattgc ctctgtttct gaaatgactc tggaaacggc gaatcaggaa
    14221 gaaaaacaag gcacctatct gtcgctgaaa ggcggagaag ttattgagca tcgaccagca
    14281 ccactgcgtc aaggaacgaa gattaccgtc agcaatctgt ttttcaatac gccagcccgc
    14341 ttgaaatacg tgaaaacact gcacacagaa ttagcgaaca tcggggacat cgtcaatcga
    14401 ctggcattga gtcatccatc gattgctttt cgtttagtac atgatggaaa caaaatgctg
    14461 gcaactgctg gaaatggcga tctgaaacaa acaattgcgg gaatctacgg attatctacc
    14521 gcaaaaaaaa tgctgaaaat cgaaggaaaa gatctagatt ttgagttatt tggctatgtt
    14581 tcattgcctg aggtgactcg tgccagtagg aattacttat cgacgatcat caatggacgt
    14641 tttatcaaga acttttcctt gaataaagca atcgttgcag gttatgggtc aaaattaatg
    14701 gtcggtcgtt tccctattgc tgtattggaa atcaaaatgg acccgctttt agtagatgta
    14761 aatgttcatc cgacgaagca ggaagtccgg ctgtcgaagg aaaaagaact aacagagcta
    14821 attagtaacg ccattcaaaa agcattgtct caagaaaatc tgattccaag tgcagcggat
    14881 aatctacggt ttaagaaaaa agtacctgat cagcctaaaa cagaacaaat cgaaatcgat
    14941 ttgtcctacg cttttgaaga agaaaaacct aaaaagccaa gtagtttgaa ttttgatcga
    15001 gagacaggta cctttttcgt agaagaacta aagaacacat ttgttgacag aattgttgat
    15061 aagtctgtgg aaaatgttga aaactattct ggtttttctg aagaaaacac cacttatcca
    15121 gaaaaaacag cgaaaacggt ggataagttt ggtgaaaatg ttgataactt tgtagaaaaa
    15181 acagtggaaa aatctgaaaa gaaagaattt cccattgtgg ataacatgga gaaaacaaat
    15241 gaagaacaag cagataaatc cgtagaagag gatgaaaaga tttacgagga agaattgaca
    15301 ctccatcctg aatttgattt cactcaagga aacgacaaaa cattagaaca agccatcaag
    15361 aaattagaaa cggaaaaacc agtacaacgt tttcctcagt tggaatattt tggacagatg
    15421 cacggaacgt atctttttgc ccaaagtagc gatgggttgt acatcattga tcagcatgct
    15481 gcccaagagc gcattaagta tgaatatttc cgggaaaaaa ttggggaagt atctgatgat
    15541 ctgcaagaat tgcttgttcc atttgtgtta gactatccaa acagtgatgc tttgaaatta
    15601 aaagagcaaa aacacttgct agaagatgca gggatttatt tagaagaatt tggtcaaaac
    15661 agctttattg tacgggccca tcctacttgg tatcccaatg gagaagaaga atcgattatt
    15721 cgggagatga tcgatatgct gctgacaact ggaagtgtga gtgtaaaaaa attccgtgaa
    15781 gcaacagcaa tcatgatgag ttgcaaacga tcgattaaag ccaaccatta tttgaatgaa
    15841 gcacaagcac gtgtgctgct gaaggatctg gcacgatgcg aaaatccttt caattgtccc
    15901 catggccgtc cagtattgat tcactttacc aattcggata tggaaaggat gttcaaacga
    15961 atccaagacc cgcattaagt caaagagaag agggaaaaca tgaagattgt attagcatcc
    16021 caatcaccaa gaagaaaaga gttattggct catatcgttt cagaattcga gattgaacct
    16081 gctgatatcg atgaaacgcc atttccagac gaacagcccg tcgattatgt tcgtagaatg
    16141 gctgaagaaa aagcacgaac tgtctgggaa caagatgaaa aagaagacca tttagtgatt
    16201 gccagtgaca cgactgttgt tctcaatcaa gagatcatgg gtaaaccaga agatctggct
    16261 gaggctgaat tcatgctgaa aaaactaagc ggggaaacac atactgtata tacagctgtt
    16321 gttttaaaaa caaaggaacg agaagaacgg atactagcag aagcacatgt cactttttat
    16381 cctttgacag acgaagagat caaacgctac ttggagacgg gggattatgc ggataaggca
    16441 ggggcatacg gcatccaaaa tgctgcgagt gtattcgtca aagaaatttc tggagattat
    16501 tacagtatcg taggatttcc gattggtgct gtcaatcagg cactgaaaac attcatttaa
    16561 acaaaaaagc ggactatctg attttcgatt gaaaatccat gatagcccgt tttttttatt
    16621 gaaaagatta cttatctact ctgactaaga ttttacttca gctttaccga ctgcttcgcc
    16681 cgggcgaaca agtcctgatt ggtcaagaga aagtcctgca actttttcat tattggtcaa
    16741 agcaacaata atgtcagttt tcttgccagc ttgttctatt tctggcaaat ccatttcagc
    16801 aatcagggtc tctggtgtga caaggtatcc ttctttcacg aatacattga aaggaacacc
    16861 ctttagttca acagtatcta atcccatgtg aacaagtact tccagcccct cattagaaag
    16921 aataccaatg gcatgttttg tttcaaagac actcgtcact ttaccgttta ctggtgcata
    16981 gactttgccg tttgacggtt cgacagcata accgtcaccc atcatttttt gtgagaaaac
    17041 tgggtcattt acttcagtta tttccttgat ggtccctgca gcgactgcga ataatgtttc
    17101 ttttgttgca actgccgtac cagttgctga aacaggttcg aaagactgtg gcgttgctgt
    17161 tgcgccattt gctgtttgaa gttgtacatc agcagcattt tcaggaacag caggatcaat
    17221 cttattcaaa attccctgac gtctaaagat gatcgtcaag ccaaatggaa tgatgacagc
    17281 aatgatcatt gcgatgatga aaggtaccca tgtttctgct tggatagcta gtattcctgg
    17341 caaaccgcct actccgatcg cgttagctcg aacacccatc aagttagcaa acatgccagc
    17401 taatccagag ccgaccattg cagcgacgaa aggatagacg tatttcaaat tgattccgaa
    17461 catagcaggt tcagttacgc ctaagtaaca agaaatcatt gctggaatcg agatttgttc
    17521 ttctttttta ttcccacgat gtaagaagat gatggctaag actgcagagc cttgggcgat
    17581 atttgataaa gcaatcatcg gccataaatt cgttgatccg aaatctgcaa tcagctgcat
    17641 atcgatggca ttggtcatat ggtggagacc agtaatcact aaaggagcat acaggaatcc
    17701 gaatacggca ctaaacaacc agctgataga agacgtcaat ccagtattta cgatatttga
    17761 gatccagctt ccgactgtcc agccgatagg acctaaaata acgtgtgcag ctaaaacagt
    17821 tggtaacaat gaaaacaatg gaacaaaaat catcgagatc gactgaggga tatatttgcg
    17881 gaagaagatt tctagatacg ctaacataaa ccctgccagc atggctggaa taacttgcgc
    17941 ttgataacca atcatctgga cttgagcaaa accaaaatcc caaaatggaa tatctgctgc
    18001 ggctgtggaa gctacactat aagcatttaa taattgcgga gaaactaacg taattcctaa
    18061 aacgattcct aatatttgtg tagtacccat cttttttgca atactccaag tgatacctac
    18121 tggaagaaaa tggaaaattg cttcacccgg taaccacaaa aaggcattga caccattcca
    18181 aaacttcgag tgttcaacga ttgttccacc aagactttca aactggatac cttcaagtac
    18241 atttctgaat ccaaggatca atccaccgac aatgattgca ggaatcagtg gtgtaaagat
    18301 ttctgctaaa acggcaatag cacgttgaac gggatgaagg ttttgttttg cagctgcttt
    18361 tccttgttct ttggaaaccc cttcaactcc agatactgca acgaagtcgt tataaaaagt
    18421 agaaacatcg tttcctatga tgacttggaa ctgtcctgca ttcgtgaaca tcccttttac
    18481 gctaggtata tcttcgatcg ctttttcgtc cgcttttcct ggatcattca ggacaaaacg
    18541 catacgagtc gcacaatgac taactgcagc aatattttct tttccgccga tatcatgtaa
    18601 cagcttctcg gcatctgctt gatattttgc catatcaata aacccctttc tcttaacttg
    18661 tctatgcaag ttgtttcttc atttactata ctttttttcg gagagaattg caagcgtttt
    18721 attaaaaagt acgagagaaa atgatatcgt tttaaaaagc ttaaaataag cttttcttaa
    18781 aaataggaac attgaaatac aaaaagagct gtagtatagt gaagaaatga tggaagaacg
    18841 agtgagggaa aaaacatgaa taagttcaat gaaatttttt tagatttgga acagaaaatt
    18901 ttagcgaaag aatatcctcc gcattcgctt ttacccagtg agaaccaatt gatcaaaatt
    18961 tatggtgttt cacgagaaac aattagaaaa gcgctgaatc ttttgactac ggaaggttat
    19021 attcaaaaaa aacaaggaaa aggttcgatc gtactggatc ggaatcgctt cgactttcct
    19081 atttctggat tgactagcta taaagagcta caggagaccc aacgtattcc aagtgaaacg
    19141 atcatccata cgttagaaga aacagaagtg acaaaagcat tgaacgagat cactggctgg
    19201 gagatcggtg cgcctgtttg gcatttgata cgcgaaagaa aaatcaatgg cgaagtagtt
    19261 atattagaca ccgattatct attaaaggaa atcgttccta ttttaactcc agcacaagca
    19321 caaggatcga tctatgaata ttttgaaaat gagctttctt tgacgattgc ttacgcgcaa
    19381 aaggagatca cagtagaaga agtaacggaa atcatgaaaa cagcgatgga tcttcacgat
    19441 gacacttatg cagtagtagt cagaagcctc gttcatctgg aagatacccg ctgtttcgag
    19501 tattctgagt cggttcaccg attagataaa ttccgtttcg ttgagtttgc ccgacgccga
    19561 aaagtgtaaa tagttcaaaa gaaagcaaat tgttggcatc tgtccacttt ggcgctatga
    19621 taggatcaca aagggggcat ttttaatgga taaaccaata agaccagaaa aagaagaact
    19681 gaaagaaaag ttatcaccca tggcttatgc agtcacacaa gaaaatgcaa cagaacgacc
    19741 atttagcggg aaatatgatg atttttacga aaaggggatc tatgtagata tcgtcagcgg
    19801 tgaaccgtta ttcagctctg cagaaaaata tgatgcaggt tgtggctggc catcattcag
    19861 caaaccaata acgaaaagac aagtgcgcga aaaagccgat ttttctcatg gcatgcatcg
    19921 aatagaagtt cgaagcaaag aagcagattc tcatctcgga catgtgttta ccgatggacc
    19981 agtagatcaa ggtggtctac gttactgcat caattctgcc tcactgaaat tcattccata
    20041 tgaaaagatg gatgaattag gttatgggga attcaaatca cttgtagaat aatggaaata
    20101 cacaaagagc ggtacaatac ggggatttac tcgatcgtcc gctctttttt tattcaagcg
    20161 attgctattt gaaagaaacg ctccctagac tccttttttc acaaggtgaa atgtgctaca
    20221 ataaagggaa cgtatgtgtg caaaagaaag aggactagaa tatgtatgaa tatctgacag
    20281 gtgtggttac ctttatcaac ccctattaca tagtagtaga aaccaatggt atcggctacc
    20341 agatcgcact aggaaatcct taccgctaca gtagcaaact aaatcaaaaa ataaaaattt
    20401 atgtccatca ggtaatacgt gaagatgcgc atcttctgta tggatttgat tcgctagaag
    20461 aaaagcaact gtttttgcga ttagtaagtg tttcaggaat cggtccgaaa agtgcccttg
    20521 caatcatggc aagcgaagac cactccggtt tgattcaagc agtagaatcc agtgatgtca
    20581 cttatttgac gaaatttcca ggagtaggga aaaagacagc acaacaaatg atcttggatc
    20641 taaaaggaaa attcggtgag ctgagtatcg atacaccttt tagtctattt gatgaagcaa
    20701 acacaaaaga cgctacagct ctatctgaag caatagaagc tctctcggct cttggttaca
    20761 gtgatcgaga aatcaaacgt gtagaaaaac aattaaaaga agtagataat cagacaacgg
    20821 atgagtatct acgccaagca ctcaagctaa tgatgaaaaa ataaggagga gaccaaatga
    20881 cagatgaata cttgttatca ccagaatctg gcgaaaatga actgagtttg gaaaaatctc
    20941 ttcgaccaca gctgctttct caatatatcg gacagagaaa agtcaaacaa gaacttcaga
    21001 tttacatcga agctgccaga aatcgggaag aacctttaga tcatgttctg ttgtacgggc
    21061 ctcctggatt aggaaaaacg acgatggcca tggtcattgc taatgagatg accgtaaata
    21121 ttcgtacgac gagtggccct gctatcgaac gtccgggtga cttagtagca atcttgaatg
    21181 aactggaagc aggggatgtc ctgtttatcg atgagatcca tcgattacct agagtggtag
    21241 aagagatgct ttattcagct atggaagatt tctatgtaga tatcatggta ggacaagggc
    21301 caaccgcaca tccggttcat ttccctcttc ctccctttac tttgattggc gcgacgacac
    21361 gggcaggtat gctttctgca cctttacgag atcgatttgg catcatttct catatggaat
    21421 attatgacga acaggatctg caggagatcg ttttacgttc ttcggatatt tttcaaacag
    21481 agatcattga agaaggggca ttggaaattg ctcgccgatc acgaggaacg cctagaatcg
    21541 ctaatcgctt gctcaaacgg gttcgtgatt ttgcgcaagt ccaaggagac gggaaaatcg
    21601 atcgtcctat agcggatcag gcacttactt tgctacaggt cgatcaggct ggattggatt
    21661 atgttgacca gaaattgttg cgaacaatga tcgagttata tggcggcgga ccagtagggt
    21721 taagtacgct ttcagtaaac ataggagaag agcgtgagac ggtagaagat atgtatgaac
    21781 cgtatctgat ccaaaaagga tttttaaagc gtacacctag agggcgaatt gctactgctt
    21841 atgcctatga acattttggt tacgactatc atatatgatt tttgctttta gattggagga
    21901 aaatgaatga gtgatacaac acgtacgaaa caattgatca aaaacgtatt gattgatttg
    21961 tgccaaacgc aatcttttcg aaaaatcagc gtccaaaaaa tcgcaaacga agcaaaaatc
    22021 aatcgacaaa cattttatta tcattttaca gataaatacg atttgcttcg ttgggtctat
    22081 tgtgaggatg cattgcatta tttagatcca caatgtcttt cacttgataa ttgggaagag
    22141 caagcattga aaatgctgaa agcaattgct gagaagaaag aattttatgc aaatactgtg
    22201 ctttctgacc cagaaatttt acaaaaagag ttttctcaac tgatccaaaa atcatttatc
    22261 aagatttttg aacaaatgga tgaagaaaga caactttctg caagagacaa actattttat
    22321 gctcgttttt tttcttacgg ttgcagtggt gtattagtca attggatcac gaatgaatat
    22381 gaagaaacac cgatggaaat cgctatgcag cttttccgtt tagccaagga tacggaatta
    22441 tattcttatc gtctttatgc aaaagaagag ttggaagaac agtaatagaa aaattttttg
    22501 ttggaaaagc aataatcgct aaaaggagat ggggaaatga caaaaatttt atttgtttgt
    22561 ttgggaaata tttgccgttc accgatggca gaaggcttgc tgagaaaacg aatagcatta
    22621 gaaggaaggg aagcagaatt ttttgtggat tctgctgcta caagcacgta tgaagttgga
    22681 aagacaccgc accctggaac gaaaaaaata ttaaaccaag aaaaagtaga tatgacgaat
    22741 atggttgctc ggcaaatcac accccacgat tttgaaactt ttgattggat cattggcatg
    22801 gatcaagaaa atgtagaaga actgaaacga agagcaccac gctcagcgca ggggaaaatc
    22861 catttgtttt taagtagcat tcctggaaaa gaaaaagaaa atgttcccga cccatactat
    22921 accaataact ttgatgagac atatcacttg atcacagaag gcctgaatca ttggtggaat
    22981 atttggattg atgaaaataa aataacaaat aatttgtaaa gttaacgctt tctttttaag
    23041 gcgatgttag agcatttcct tgtttttttg taaaatgtta ggtaaaataa aattaattaa
    23101 gtaacacttt cttagctaaa ggaggagtgc attcgatgaa cgaagagaca gtatcgccaa
    23161 tgaagaagct tacgaagtta gttactcata cttcgtatca actttttgat tcttttgtaa
    23221 atgacactga agaacatgct aagaatgtac gattgcaagg aaagtttatt cttgagctgc
    23281 aaaaagctgc tactgaacat agtctagtta tcttacaagt aagagcaaat cgtttgagta
    23341 gcaagtatga aacgatctca ggctgggtgg cgacaaaaac ggtcgaaaaa gaccgagtga
    23401 tgatccgcct acaaaacgaa gagcagcaga tccggatggt tcctgtagaa caaattgaaa
    23461 aaataacaat cctttcacca gcaggggaac aggaacggtt atcaaaataa tttataccag
    23521 atagaaaagc acgtatgagc ttgtaccatc aatcattact aatcaaaaat aaccgaatgc
    23581 tttaagctga tatcagtaaa acggaacgtt ttaggatgag tatcatagca tgacagcatt
    23641 cggttgatta gttgaatcga ttggatggta caagtttttt tgataaaaaa aggtcctgct
    23701 agccggggag ctagcaggat aaggagttaa aaatgaaaaa gtgttgttag ggttgtttgt
    23761 tggtatgctt atataatagt cattagttgt gaatattttg tgacgaaagt tttcgaaagt
    23821 tgttaagttt t
//
LOCUS       DACDTO010000037        23076 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDTO010000037 DACDTO010000000
VERSION     DACDTO010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.037"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            102..2543
                     /locus_tag="IWK70_001488"
     CDS             102..2543
                     /locus_tag="IWK70_001488"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9614021.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="IWK70_001489"
     CDS             complement(2873..3577)
                     /locus_tag="IWK70_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614022.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3764..4009)
                     /locus_tag="IWK70_001490"
     CDS             complement(3764..4009)
                     /locus_tag="IWK70_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614023.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4026..4877)
                     /locus_tag="IWK70_001491"
     CDS             complement(4026..4877)
                     /locus_tag="IWK70_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9614024.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5043..6047
                     /locus_tag="IWK70_001492"
     CDS             5043..6047
                     /locus_tag="IWK70_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9614025.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6288..6644)
                     /locus_tag="IWK70_001493"
     CDS             complement(6288..6644)
                     /locus_tag="IWK70_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9614026.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7012..8199
                     /gene="tuf"
                     /locus_tag="IWK70_001494"
     CDS             7012..8199
                     /gene="tuf"
                     /locus_tag="IWK70_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9614027.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="IWK70_001495"
     CDS             complement(8387..10978)
                     /gene="mprF"
                     /locus_tag="IWK70_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9614028.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11004..11723)
                     /locus_tag="IWK70_001496"
     CDS             complement(11004..11723)
                     /locus_tag="IWK70_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9614029.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12043..12564
                     /locus_tag="IWK70_001497"
     CDS             12043..12564
                     /locus_tag="IWK70_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9614030.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12623..13483
                     /locus_tag="IWK70_001498"
     CDS             12623..13483
                     /locus_tag="IWK70_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9614031.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13590..15983
                     /locus_tag="IWK70_001499"
     CDS             13590..15983
                     /locus_tag="IWK70_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9614032.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16165..18006
                     /locus_tag="IWK70_001500"
     CDS             16165..18006
                     /locus_tag="IWK70_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9614033.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18030..19139
                     /gene="rpoD"
                     /locus_tag="IWK70_001501"
     CDS             18030..19139
                     /gene="rpoD"
                     /locus_tag="IWK70_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9614034.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19359..21458
                     /locus_tag="IWK70_001502"
     CDS             19359..21458
                     /locus_tag="IWK70_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9614035.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21654..22517
                     /locus_tag="IWK70_001503"
     CDS             21654..22517
                     /locus_tag="IWK70_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9614036.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22622..>23076
                     /locus_tag="IWK70_001504"
     CDS             22622..>23076
                     /locus_tag="IWK70_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9614037.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7770 a         4038 c         4810 g         6458 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaaaa
     3721 atggaacgga ttatgtatat atacataatc cgttccattc tttctatttt ttcggtatca
     3781 aggcaaaaac aggtagttca ggtgaataaa tgacttcatg ttcgtgccag ggtttgacca
     3841 ttagcgaatg atcgaaccgt tttttcagtt cttcgatgat ttgatcatgt gtccagtttc
     3901 ttgcaggaaa atgttggatt ttctctgcat cgatcaaaaa gacaaaatcg gaatgaaaag
     3961 tctgggtaac ttcttccgag agagaattca agcgttcgat tggatccata aaatcacctt
     4021 tcgacttaaa attgggtagt atctgggtct tttgctttgc cgactacacc atctaactga
     4081 tcgatttgtt tcatttcttc ttctgttaat tcaaaatcaa ataattcagt attttctttg
     4141 attctgcttg gtgtcactga cttaggaaga ggaaggaatt cgtgttgcag cgaccaacgc
     4201 aaagcaactt gtgcaatcgt cttgtcatgt gcttcagcga tttgtttcat ttcaggcaca
     4261 tcgaagattt taccagtgcc taatggacta tatgcttcga ggatcatatc atgttttttt
     4321 gcatactcaa ctacagttgt ctgtaattcg cctggtgcaa ggaaaacttg attgaccatc
     4381 ggcataattt ttgctgtttt tgctagtgta tctagatggt ggggtaagaa attgctcaca
     4441 ccgattgctt tgattttacc tgcttcatac agttcttcaa atgctcgcca tgtatctgca
     4501 tttgcttgtt cccaattatc acgaaaagca acagggtttg gccagtggat caaaaacaga
     4561 tctaaataat ctgtttggag cttcttcaat gattcttcaa aagaagacat cacaagttca
     4621 tagctgtggt ttgcattcca taattttgtt gtcaagaata tctcttctct aggaattcca
     4681 ctttccttaa tcgcttggcc gacgctttct tcattcttgt atccttgggc agtatcgata
     4741 tggcgataac ctgcagccag cgcttcttta acagaggaaa cagcgacatc cccatcaggt
     4801 gtttgccatg tgccgaatcc tactaccggg atttcgtaac catttgatag acgataagtg
     4861 cttgttaatg aattcatact ttcagctcct tttttattcg ttcaactata agtgtagcgc
     4921 atttttcgaa aaaaagaatc cattttgttt ctactcttga aatttttgtt taaaagaaaa
     4981 tgattttgat tctggtattt ttgatttttt ttccgtaaac taagacagaa gggaatgaga
     5041 aaatggatat ctatttaaaa aaagcaattc tgcatattat tgacagagaa actggttctc
     5101 cagtcttttc acaaaaagag ctggatctga caaaagaata tattcgtgat tttcttcaaa
     5161 agaagatcca aaaaatctct tctgctcaaa caaaaactgg acaactagca gaagaaagca
     5221 tgatcgttga atcacttaaa atcgctcaag aaaattttgt ggaagcaagt gaagcaatcg
     5281 ttcagcggtg gttcgatatc taccaagaaa gtgaagaagc acctagtgca gatgtatttg
     5341 ttgtcttgta tgaattagat acagtgatgt acttatcttt attgaaagtc aattatcgtg
     5401 aggcttacac tcactttgtt gaagcagacg aagcagggat cgacaaccgc ttgattttga
     5461 atcgagcaat tctaggggcg aaaacacaaa aagcagatga agcattcgct gtgaatttga
     5521 ctgattggac ctacgaatta attgaaaaaa aatatgagtt ttctggcaaa aaagagcttt
     5581 atttttctgg aagagtgatc gaaagtgttc ctgcaccttc tttagaagaa aatgtaaaag
     5641 tgatccaaaa agtagccaag catcttggaa aaaaatttga gacagaagaa tttgatatca
     5701 tggctgattt gaaagaagct gtctatgata cgatagaaga aaaaggtcgg ttggatcatg
     5761 agatgattgc ggaaaaagtc ttcaaggaaa acatcacggc aaaaatggct tttaaagaag
     5821 aagtacaaga gcaaggattt gtcccagaag cacctcctgt aaaagaagta caagaaattt
     5881 ctgagaaaaa atttggaaaa caaaaattca agctatcaaa cgggatcgaa ctgatcgtac
     5941 cagtagatgt atatcgcaac ccagatttga ttgaatttgt gaataatccg gatggtacga
     6001 tatccgtgat gatcaaaaat gtggatgaag tattgaatcg tttataaaaa tgactagaat
     6061 attaggtgtt caagtaccag tgatcagtta aatgaattgt gtcaagagaa ggatcggaaa
     6121 agacgaatat aacaaaaaaa gagcgtgaga caaaagtaaa gaacactttt gtctcacgct
     6181 ctaaacacgt ataaacggcg gaagcagaag caactccttc ttatttcttg gagttaaacg
     6241 cttctgtccc agcctctttt tatctaataa aattttaaga ccatttacta attatcgatt
     6301 ttttctgccg catttttagg cacttcagaa gcctttattt cttcccagct taatacacct
     6361 ttacgaggat tgactttgag tttaagatac gcattcatct ttaatggatg ttctctttct
     6421 tcgcgaagcg tcacctgttt tttttcacca tctttattat aagccgcttg cttgtaggta
     6481 taagtcgtga atttctctcc gttatctgct tgggtagctg acatttcgcc attcgtcgtg
     6541 atttttgtat aataagcttc gccgccgtaa aaataatgat aagctgcaaa acttccgccg
     6601 agaaatatgc ctaaaacgac aagtacgcca attaattttt tcatgtactt cacctctttc
     6661 cttattatta acgaaataga agatttcgtc tattgaaagt tgttggcaac agctgaattt
     6721 taaacttttc ctcattttgt tcttattgct tcaaaagaaa gttaaggact atcttgaagt
     6781 ttttctcaaa aaacacattt ttttatttat aaaaggtact tttttcgcgt ataagacaga
     6841 cgagtgctct tagttgactt tgaccaatat tgacgtataa taagcttagt tcgaatctta
     6901 caaaaagtaa gtcggacgta ttctttttga tttagtcaaa aaaagactat aataaaaagg
     6961 tgagagctga aatttttcag tgatctatat tatttaggag gtttgtaaat tatggcaaaa
     7021 gaacattatg acagaagtaa accacacgtt aacattggta cgattggaca cgttgaccat
     7081 gggaaaacta ctttaactgc tgcgatcact acagtattag gcaaaaaagg cctagcaaat
     7141 cctcaagact atgctagtat tgatgcagct ccagaagaac gtgaacgtgg gattacgatc
     7201 aataccgcac acgtagaata tgaaacagaa aaacgccact acgcgcatat tgatgcacca
     7261 ggacacgcgg actatgtgaa aaacatgatt acaggggcag cacaaatgga tggtgcaatc
     7321 ttagttgttt ctgcaactga cggtccgatg cctcaaacac gtgaacacat tttattgtca
     7381 cgccaagttg gtgtaaaata cctgattgtt ttcttgaaca aagttgattt agtcgatgat
     7441 gaagaattga tcgatttggt agaaatggaa gttcgcgagt tattgagcga atatggtttc
     7501 ccaggcgatg acactcctgt gatcaaaggt tccgcattaa aagcattgca aggcgatcca
     7561 gatgctgaag ctgctattat ggaattgatg gatacagtag atgaatatat cccaacacca
     7621 gaacgtgata cagataaacc attactattg ccagtggaag acgtcttctc aattacaggt
     7681 cgaggaactg ttgcctcagg tcgtattgat cgtggtgctg ttcgtgtcgg tgatgaggta
     7741 gagatcgtag ggatcaaacc tgaaacacaa aaagcagttg taacaggtgt agaaatgttc
     7801 cgtaaaacgt tagattacgg ggaagctggg gataacgtag gcgtgttgtt acgggggatc
     7861 caacgtgacg atatcgaacg tggacaagta cttgctaaac caggttccat tactccacat
     7921 acaaaattca aagcagaagt gtacgtgttg acaaaagaag aaggtggacg tcatactcca
     7981 ttcttcaaca actatcgtcc acagttctac ttccgcacaa ctgatgttac aggaacaatc
     8041 acattgccag aagatacaga aatggtcatg cctggtgaca acgtgacgat tgatgttgac
     8101 ttgatccatc caatcgctgt tgaaaacgga acgacattct caattcgtga aggcggacgg
     8161 acagtaggtt caggtatcgt tacagagatc gaagcataag aaacgcgact cgaatatttt
     8221 ggtaatgaaa aatgtttaaa ataaaagagc ggtccaaaca ggatttctgt ttggaccgct
     8281 ctttgttctt caattgtata agaagagatt gtagaagcat gaactttcgt tttacttcag
     8341 tctcttttat tcatcatgag agtgttcatc tatcggtatg cagtttttaa tactttcttc
     8401 gtatccattt tttcaaacca cttattctac cttgtgtctg ctcaactggc gcattatcaa
     8461 taatcagcaa agcaatcata acatatgcaa tccaactatc tctagaatat agagtataac
     8521 gtgagaccca gtttgtagcg tacttgcttt tatattctct taatccatgg aaagaataaa
     8581 aatgagaacc aaactcgtaa accaagtatg caatcctttc ctgtaaaaaa ctctttctgg
     8641 aagttccgac atttgaaaga ggggccatcc ctaaattgaa ccattggata tgttcttctt
     8701 tcatataggc aaacaaatgc aagaacaaga aatccatact gccagaaggt gcttttttag
     8761 aatcatatcg catcagatca atcgttccta ctttattgtt cgtgtaagta ggcatgatcg
     8821 ttgcaaaagc gacaatttct tcttgggtat ttttgacaac tgcaataggc gctctttgta
     8881 gataatcttc agagaaaaag cctaaagaaa accctttttc ctttcgtgaa cccaaccaat
     8941 tatcagagat atttttgaaa gtactcattt gctcagtaga aaatggtggt tgtaagacat
     9001 caaatgtaaa tccttctcgt tcgatccgat tcaagacagc acgagtgccc ttcatttttt
     9061 tccctgaagt tgtaaaacta ttcaggtcta cataagcttc ttcccccatc ttaatgaaat
     9121 cgtagccgaa ttcatgcaaa atcataacaa tctcttcgct tgtttcgtag aaaactggta
     9181 aatagcatag tcgatctgtt tcttcgataa aagcttcgat tgcttctgga aaatcttctt
     9241 tcttaccaga aggatcaccc attacaatac atttattatt gaagcaagcg aattggagaa
     9301 gaacagttgg ttcaccgtct ttttcatagg caaacatccg cttatctttt aagaagatca
     9361 attgactatc ggagtttcct ccataagttg taaggatctt cagggccttt tcttcattaa
     9421 aggcttcgcc aatctgtttc ttttctcctt gaaggaaatg aacaaataga acgatcatga
     9481 aactaacagc gatgatggcc aacaatcccg aaaaccaaat cttttcagaa ggaaataaga
     9541 aaaaggaaat aaaatggtga cgccgatgag ggaaatccgg cagattgtat accccaataa
     9601 cgatgtaaag caaggacagt gcaccaataa tgattccatc gatcgtacgc cactcccatg
     9661 aatagatcaa ttgttcccga aataattcgt ttttagaagc aagaatgatc agaagcagaa
     9721 tagacaagaa aaccaccggt gtgaaactaa aatcacttaa taaaacataa agtaaagcta
     9781 gaacaatcaa gaagattgta ggcaaatatg ctcgtttcac acgtgctgcg atccctctgc
     9841 ccataatgat taaaagaaat cccaatagta tactaggaaa ttggataata aaatgaaatt
     9901 tcaatgggtt caaactatga agccagcgaa actctgtaaa agcttgcggg atagtggcag
     9961 aaagtactag catgattcca gaaaagtata ataagacaac cacgatttta tgcgcaattt
    10021 ctgttgctaa ttgtttaggg ataccagaat aacgctgatc aaaagtggaa ccgatatttt
    10081 tgaagaaaaa gacgattcca atcaaaaacg gaatgatgta gtagaacagg cgatagagca
    10141 agatccacac aacgactatt tctcttggca caccaatagc ggacaacccg ataatcatca
    10201 ttacatcgaa actcccaatt tctcctggaa tcatagacac gattcctatc actgaagcag
    10261 caatgaataa tggaattgtt tgccacagat caatccggat ttccattaaa taaccaacag
    10321 aaaggaaact aaacaggaca ccggtccatt ccaacaatga aactagcaat agctgtcctt
    10381 gagttttaag tgaggtacca ccaaggaatc ctttcttttt gaaagtagta aataaaaaga
    10441 cgacaggaaa atatagaccg ccgccgatca accatatcca atattgcttc aaaaaaggag
    10501 caactggcgt gaagactaca agtaaaaaag aaagcagact gtaaatagaa agtccagcca
    10561 tcaaaaacaa gaaaactttc gataatgctt ggatcacgtt tctgctctct ttcttttgtc
    10621 catatagttc agagcgaagc cccacactaa caaacccgcc gaaaccggca atattgttga
    10681 tcgtattgat cagccagctt gtctcaaaca ggtaacgagg attctgcttt tgttcaagca
    10741 tccgattcaa aataatatca taaccaacca taggtaacac agagataaga ccaatcaata
    10801 gcattagtcc gcttttccat acaggtatag tggcaaacgt ttctcctaat tgctggatag
    10861 atagagtttt accgatggac atcaattcac cgacaataat gacaatgaca gaaatcaaaa
    10921 agattgtttt gaacagtcct aggtggtttt tcagccattg tatggtattt tttaacaaat
    10981 aaatcacctt ctattcctaa tagttatgtt tcagaaagaa attgaagaac ttctcgattg
    11041 aagcgttcag gatcttttct agctaattca tgtccttgtt ctttcacaag cacaaaggaa
    11101 gcttttggta tagttttagc tatatacaaa gaatgtttta gcttgattac atcttttttt
    11161 cccacgatga tcaaagtggg ggagttgatt ttttttaaat cattttctgt aagcccgatg
    11221 tcatgcagca aaagtttgat aactaaaaga tttttgcgaa gacttgggcg aaataaagag
    11281 agtaaccata cccatgcata atgaatatta ctgatgactc tggcagaaaa acgaacgcct
    11341 ttaacaagcg tgtttccaga atttaaaatc aatcgatgaa cttttttagg gaagctgctg
    11401 gcaaatacca gtgctagatt tgctccatca ctaaatccaa gaaaatctgc ctgatcgatt
    11461 ttttccagca acatgatcgt attcagatcc tcggccatta actgaaaatt caacatagat
    11521 gcttcgtttg tcgaacgacc atgtcctcgg ctatctacta gatacacggt atagtagcgt
    11581 tcgagtacag ggacctgttc tgaaaaaaag cggccgctgc cgtcattgcc atgcagtaaa
    11641 aagagaggga agccttgacc gctcttttca tagtaaattt cgctcccatc tggcattttt
    11701 gcatatttct tttctttttt cacataatca cctactgtgt aaagtataac gattatatca
    11761 gtcattgggc aattgttttt actgaaaaac aacgatcatt ttcgtaagat gtcaaaaaat
    11821 acaggaaaat tcattttttc ttaaggcgta cacagaaatc aaggaagaaa tagccaagaa
    11881 gataaagaaa tagtaagata aaaaaagatg tcaaaataac taggtgatga aatgaagcga
    11941 aaacgattat ctattatccg tattcttttt tacatgatca ttctgacggt tattggtgcg
    12001 gtgttgtttt tatgaggaag aacttatcgt tcggtaaaac gagtggaaag ctatcaaata
    12061 gaaataacaa cagctgcaaa aaattatcat atggaaaact acgagaattt gatcaaggcg
    12121 ataatattga ccgaatcaaa aggaaaaggg acagatctca tgcagagctc ggaaagtctg
    12181 catggagaag ctgagatcat tgataatcca gctgacagta ttgatcaagg tgtcaaatat
    12241 ttggcggaaa tgatagtaga agcaaagaaa caaggatgcg atttatggac agctgtccaa
    12301 gcatataatt ttgggaaaga ttatattgcc tatgtaaaaa atcatggcgg agaaaacaca
    12361 gtagaattag cagaagcgta ttctaaagat gtgctgagcc cacttttagg aaatgaggaa
    12421 gggaaacaat accgatattg gcggatccag tccttgcttt acaatggcgg ctatctctat
    12481 cataatggag gtaacatgtt ttatcctgat attgtcaaaa tgaatgaaaa aataatagag
    12541 acctatgaaa aagtctttaa gtaaaatagc aggaaatgtt caatcatgaa taaaattaaa
    12601 tgaaagaagg agtgcgcaaa tagtggaaaa aaggcaaaaa atggaaaatc aagaagtggg
    12661 gtacacaacc aaagactgga tgttacggtt tgtgaaggga atgtttatcg gttcaggatt
    12721 tatcttgcca ggagtcagcg gtggtgcatt agcagctgtc tttggcatgt atgagcggct
    12781 gatttctttt ttagcccacc ttacaaaaaa tttcaaagaa aatgttttgt tttttctgcc
    12841 tgttggtcta ggcggagtga caggcatctt tctactttct tttttagtca gttttttatt
    12901 aggtgcgtat gaaacaacga tattatggtt ttttgtcgga tgtatcgctg gaactgttcc
    12961 tgcgttatgg aaagaagcag ggaaaaaagg aagagatact tctgatgtga tcatcatggt
    13021 tgttactttt gttctggctt atttgttttt attatacggc gctcaattat ttgacgggca
    13081 agtcgatcag aattttggaa catggctgat tgccggcgga ttgattggtt tgggtatgat
    13141 cgtcccagga ttgagccctt ctaatttttt ggtgtatatg ggattgtata aagcgatggc
    13201 tgacggattt aaagaagtac gacttgaagt gattgtgccg atcggaattg gtgggattct
    13261 ttgtgttctt ggattgtcaa aaatcatgga ttacattttc tcaaaggcat atagcaagct
    13321 attccatttc attttaggtg tagtttttgc ttcaacgatt atgatcattc ctaccgatta
    13381 cgcgggattg ggagttgctg gggtactagc ttgtcctgtt ctttttatag ccggtacagc
    13441 actaggttgg tggatgagcc gtttggagga acaatataag taaagataga ttgataataa
    13501 aaacagcaca cgggtgattc aaacaaaaag gttggcatct tttttgtaaa atcattcgtg
    13561 tgtttttcta taaattttat aaaaacaagt tgtttttaat cttggaacac gctagactta
    13621 aacacgtgat tatttttgaa agttggcgga aaatggagaa aaagaaaatg aatggactta
    13681 cacagcaaga ggtagttcag aaaatggctt ctggccagca aaatgactac caggaagatg
    13741 catcaaaaag tacaaaacaa atattcagcg ataatatatt gacgttattc aattttctga
    13801 actttggtat tgggatttgt ctgttatttg taggtgcgta ttccaacatg gcgtatttag
    13861 caattattgt cgtcaatatc gttattggaa tttatcaaga aatacatgca agaaatcttg
    13921 tacggaaatt atcgattgtt tcccaatcag aagtactagt gatccgggac ggtaaagaac
    13981 aatcgatttc tactaaagag ctggtattag gagatttggt aaaaatcgga gcaggagaac
    14041 agataccagc cgatttacag gttatatctg gacgggcaga agcaaatgaa gctcttttga
    14101 caggcgaatc tgatttgatt gtcaaagaaa ctggagctga attgctttct ggaagctttt
    14161 tgacaagcgg ttctgtcatg gctgaagtta tccgagtagg tgcagataat tatgctgtcc
    14221 gcttgactag cgaggcaaag actcacaaag ctattcgttc tgaacttgta gattcgattc
    14281 gcaaagtatc caaattcact agtttcgtga ttattccttt ggggatcata ctatttttgg
    14341 aagcactgtt catccgcgat gcttctatca aaatttctgt tattgcatct gcagcagctt
    14401 tgttaggcat gcttccaaag ggtttggtat tgctaataag tattgcatta acgacagggg
    14461 taaccaaact ggcaaaaaaa cgaatccttg ttcaagacat gtattcaatt gaaacgctgg
    14521 cccatgtcga tacattatgc ttggataaaa cagggaccat tactgaaggg aaaatgagta
    14581 tccagcgggt agaagcacta catccgctgt acgagaaagc catcccagaa attatgggga
    14641 cgtatctggc agaaagcagt gataacaaca tcactatgcg ggctatgaga gattattttg
    14701 agctttctaa tcggtatgat gtgacaaaca aagtcgcatt ttcttctgat cgcaaatggg
    14761 gcgcgatgga atttccagaa ctgggagtcg tgtacgtagg agcgcctgaa cgtttggtgg
    14821 aagaagatcg gcttccggac agtctaaaaa cagcccaagc gaatggctat cgcgtactta
    14881 tgttggggat cgctccaaat cagcaattga acgaagaaaa accagcattt gttgagccgg
    14941 ttgctctgtt tgaaatcgat gatccaatcc gaaaaaacgc tcaagaaaca ctagcttatc
    15001 ttcatgagga aggtgtggat ctgaaagtga tttctggaga caatcctttt actgtctcca
    15061 atatcgctag acgtgcagga ttaccgaatt atgatgcgta tgtcgatctt tctcaaatta
    15121 ctgaagaaat ggaagtgcga gaagctgctc atcgctatac ggtttttgga cgagtgagtc
    15181 cgcagcaaaa gaaattgctg gtaaatgaat tgaaagaaag cggcagaacg gttgcaatga
    15241 caggcgacgg tgtgaatgat gttttggcgt taagagaagc agattgcagc attgctatgg
    15301 cagaaggtga tggagcaaca cgtcagattg cgaatctggt attgttggac tctgatttta
    15361 ctacattgcc agaagtcctg tttgaaggac gccgagtggt caacaatgtg acgaaagtct
    15421 caggcatctt ttttatcaaa acgatttatt catttatttt atcgattata tgtgccgtta
    15481 cagcaattgg atttccgttt atccctatcc aaatcacgct gattgattta gcgattgaag
    15541 ggtatccttc cttcttttta tcttttgaac aagataaaag aaaaatcact taccgttatt
    15601 tgcctacagc acttattaat gctttaccaa atgccttgct tgtagtagtg aatatcattg
    15661 cggtgtatct acttggaaaa acattcgcat ggggaaatgc tgaaactact actttgatgt
    15721 attacttgct gattgggatc agttgcatgg cagttgtaaa agcttgttat cccttcaacc
    15781 cattgcgcat cttcctagca gtaacgacga tcgtagggat atatgtagct gcaatgctgt
    15841 tccatggaat tttggaagta aacttagggt tgggagagag cgtaggatac ttctttgtat
    15901 tcatgctcgt aaatattgtt cttcgagtat tgtactggaa ggctgatatt caaaatcagt
    15961 tactgaaaag agtagcaaat tgatgcttaa tgagatagaa atgtgttata cttgaaaaag
    16021 caatgaaata tgatacaatc aaacatgtat aattgtgtcc tttaaaatcg cactgatttc
    16081 agttagtgcg attttttacc ggttattctc ctgaaatatc ttctgttaaa cccaaaagat
    16141 atcaatagag cagggggtga aaaaatggct caacggattc ctcaagaggt tatcgaaaca
    16201 gtacgcagtc aaacgaatat cgttgaggtg attggacaat atgtccagtt gaaaaagtct
    16261 ggtaaaaatt acttggggct gtgtcctttt catgaagaac gtacaccgtc tttttctgtt
    16321 gcagaagaca agcagatttt tcattgcttt ggttgtggta aaggcggaaa cgtgtttact
    16381 tttcttcaag aactagaagg tctaagtttt ccagaagcag tggtaaaagt ggcagacttc
    16441 gaacagatac cgttagaaga tcaatggcgt cagcgtacta tagctgtcca aaatcctgaa
    16501 tctgctacgg ggaaactgat tgctgctcat gaaaaagcag cggaaatcta tcatcacatg
    16561 ctattgcata caaaagctgg acagccagct ttagaatatt tgcaaagtcg tgggttgtct
    16621 ttagaattaa ttgaagaatt caacataggt tttgcaccta atgagcgttc ttttcttcag
    16681 caagtattca agaatgaatc aatttcaaat gaattgttag aacgctctgg attgtttgtc
    16741 acgcatgatg atggccgtat gtctgatcgt ttttatcaaa gagtgatgtt tccacttcgt
    16801 aattctcaag gccagacgat cggtttttca ggacgctggc ttgcacctga aaaagggcag
    16861 gaaaaagatc agcccaaata cttgaatagt ccagaaacag aactgttcaa taaaagacaa
    16921 gttttattta atcttgataa agcaagaagc gatatccgga aaaacggaga aattttgttg
    16981 ttcgaagggt ttatggatgt gattgctgca tggcaggctg gtatcaaaac tggattagct
    17041 tctatgggga caagtctaac agctcaacag atccaacaaa tggaaaagct gacgaaagaa
    17101 ttggtctttt gctatgacgg agacaacgca ggttttgaag caaccaatcg agggattgaa
    17161 ttgcttcggc aaaacagtcg attagcactt tctgtcgtag tcgttccaga aaagcttgat
    17221 cctgatgaat atctccgtaa gtatggagaa gcatctttcc aagaattagt ggctcatggg
    17281 cgcgaaacag tctttacttt taaatcacgt tatttaaaac aagggaaaaa tttagataat
    17341 gaaaaagaca agatcgaata cctcgaagaa ttactagttg aacttagccg agttgtttca
    17401 ccaatcgaac aagatatgta tctatctcaa ctgtcaacag agtttcagat cggcagagag
    17461 acgatacaaa aacaactgcg cgatcttcga aggacaaatc agcaaaatcg tcaagaacct
    17521 caggaaagtc aacacactca ggttacgaga caagaaactg cacggaaaaa gagacctttg
    17581 acgcaagtag aaaaggcaga gcagattcta ttatatcgtg catttaaaga agtttctgtc
    17641 cgtaatattt taaaagaacg agaatttcag tttattcatg atgcatatgc agaagtttat
    17701 tttttattcg atgcatatgt ctcacaacat tatgaattca accttgcaga gtttttagac
    17761 tttttgcaag atgaaaattt aaggcggttg gtggtggaaa tcgaatattt accggtggca
    17821 gaagaaagtt cgccacgaga aatccaagac ttgctagatg tgatcagcaa atctggctta
    17881 gctgatgaaa taacattaaa aaaacaaatg cagcaagaag ccagacgaac tggaaacaaa
    17941 cagcttgagc ttgaattgac gattgaaatc atcaatttaa cgaaacagtt aaaacaagcc
    18001 aaatagctac tcaagggggg ccttctttta tggcaaatgg aacagataaa aaaacgtatg
    18061 aagcagcagt agcagctttc atcaaagaaa acaaacccaa acgacaagtt gtatatgatg
    18121 atttatccaa tcagcttgct acaccattca ctttgaatgc agatgaaatg gacaaattga
    18181 tccaaaagat tgaagacgca ggtatcagtg tggtggatga aaatggtgat ccatcaattc
    18241 acagcttgaa gagtgctgag aaaaaagctg aacaagcaaa aacagaagat ctttctgcgc
    18301 caaccggtgt aaaaatcaat gacccggttc gtatgtatct gaaagaaatt ggccgtgtct
    18361 cccttttaac tgcggaagaa gaagtagcgt tagcattgaa aatcgaagaa ggcgaccaag
    18421 aagcgaaaca gcgtctggca gaagcaaacc ttcgtttagt agtaagtatc gctaaacgtt
    18481 atgtaggacg aggcatgcag ttccttgact tgatccaaga aggaaacatg ggactgatga
    18541 aggcagttga aaaatttgac taccgcaaag gatttaagtt ttcaacctat gctacatggt
    18601 ggattcgtca agccatcact cgagcaatcg ctgaccaagc acgtacgatc cgtattccag
    18661 ttcatatggt tgaaacaatt aataaattga tacgtatcca acgtcaattg ctgcaagacc
    18721 ttggaagaga acctacgcca gaagaaatcg gtgcggaaat ggatcttcca acagaaaaag
    18781 tccgagaaat cttgaaaatc gcgcaagaac ctgtttccct tgaaacgcca atcggtgaag
    18841 aagatgactc tcacttaggt gatttcatag aagaccagga agcaacaagt ccagcagaac
    18901 acgcagctta tgagttacta aaagaacaat tggaagatgt actagataca ttgactgatc
    18961 gggaagagaa tgtccttcgc ttacgttttg gtttagatga tggccggacg agaacattgg
    19021 aagaagtagg gaaagtattt ggtgttactc gggaacgtat tcgtcagatc gaggctaaag
    19081 cattaagaaa attgcgccat ccttctcgtt caaaacaatt aaaagatttc ttagaataag
    19141 cgtggactat tgaccagaag agaaatcagt aaaactgttt ggtttatttt aaaagaaaaa
    19201 aagcttgaga ctacgcagtc tcaagctttt ttttctatag gaaggaaatt cattcattat
    19261 ttttaagtaa atcctaaaaa taagctattg gcgtgacgta tagaatatga tagattaaat
    19321 ggtagcgtaa acctagtaaa ggagccgatc gctaagtgat gaaaaaatgt ccaaattgcg
    19381 gatcgaaaaa aatggagcag aatactatct gtccagactg cggtcatcaa ataactgcag
    19441 aagatcagca aataaaagaa tcaattgaag aaacaaatac agatgcagct atgcaggata
    19501 aatcaactga agatgcagtc tttcctgata cagaattaaa cgatccaatc gaatggtcgg
    19561 aattgaaaga tttacctcta gaatctgtaa tggaattatt cgaaaaagca gaaccagaag
    19621 acgaagaacc acctgctcac gatcaagtaa ctaatataga gaaaaatgaa cagaagaaag
    19681 caactgttca tgagacttcg gaagaatcag gaaagaatca agaagaaacg cttgaatcaa
    19741 aacaaaaaag aagagtagct gaactgaaag aaatcgtcga taatgaggaa gaaaattcga
    19801 tactatccgc ttatatcaaa gcacatcgtg aagatacaaa agaagagcac gcgaaagagc
    19861 tgctcaaaat gatcagtgaa aaaattgcac aggaaaacaa agtagagcca gaaaaggaac
    19921 aagaaactga aaaagactct gatagagaaa acgccacagg taaagaaaca gtaaaaataa
    19981 aagacgtaaa aacggatatc acagaaaaaa ctgaagtatt tactgaaaaa caattagaaa
    20041 aggaaaaaac gaataaactg ccggaagcag agaaaaaaga aatactcgaa aacacagaca
    20101 aaaagtcaac agagcccgat gaaataaata gtgaatccga tgctgaaaat caatctgtaa
    20161 gcaaagaggg tactgatctt gaagcaaaaa tagaaaaaga agagaaccag cctaagctgt
    20221 ttgaagcaga agcgaaatca ataacaatcg tgccagatac tgaaaaaaca catcaagaat
    20281 caaacttaga aaaaacagat ccagataaag cgcaaaagaa aaaatcaaaa aaagtacctt
    20341 atctcgtttt agcagcagta cttctactcg gtggaggaag ctgggcttat tatgaccatc
    20401 atcaaaaagt agaagccgaa gtagcagcag aagcaaaaag acagcaacaa atggaagaac
    20461 ttcatgagaa acttgctgcc ttttatacag atgatcacca tcaatttatt cgtgcagata
    20521 tgattcatca agatttatca aaactaaaaa atgatttatc cagtcttaag aatgaaaaag
    20581 gatattcaga gttagaaaca acttaccaag atatccaatc taagatccga gatatccaaa
    20641 aagtgaatga gtggttcata gcacctgcca tcgtcgatga tcatctagca gatgacatca
    20701 agttgaaagc agataaagag atccaagcaa tcgaaacgga taacacagat tttggaaaat
    20761 tgattcaaca agcacaaaca gaagctaaaa atcaatatgc tgaattgcaa acagctaaag
    20821 aaaaaacagc tatagtcttt aaagacggaa aagtagtgga ttcagcaaca cgcgaccaat
    20881 acaagacggc aaaagaagca gtagaaaaag tgaaaaatac agatctatct gctcagttga
    20941 atgaacaatt gaaacaagta gataagatgc tcactgataa agagaaaaaa gaagaagagg
    21001 aaaagaaaaa agcagaagaa gcgaaaaaag cagctgaaga acaagcaaaa caagccgctc
    21061 aagcagcagc tgcagctcaa gccgctcaac aagctgcggt cgcacaagca gcacagcaag
    21121 aagcggcatc ctcgacccct tcctcttcaa actcagcgaa tcagccaatc aaatctgcca
    21181 gagcaagtga tattgccgat gcatctaatc cagcatggac gtgggcacca ggtgtccaaa
    21241 gcaaagtgat tgctacatgt atccaacgtg gatatatcgt tgaaggaggt tacagattag
    21301 aaaaagccag aatcgaaaat ggggaaggct attataatct atatgccact tcaacaaaat
    21361 ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt ttacatcgta accatcaact
    21421 gtaaaactgg atggttcgga ggaaatggga gcaattaatt ttaggcaaat gaaagaggct
    21481 gtgagcctgc cgtttaacac cgtttattcg aaaaagggga gtatgacaca actgttgtca
    21541 tactcctttt ttcacctttc caaaccaagg ctcagctatt caagtagata acattatgtt
    21601 atgatagcaa agaaatgtat taagaaaacc tttcactata gagggagaaa aaaatgaacg
    21661 atttattagc aactatattt acaggtatta ttattgatga aaatgaaaaa aactactttg
    21721 tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga aggtgaacac aaaatcggtg
    21781 aagcagttga aggattcggt taccaaaatc aaaaacatga aaatcggatc actacacaaa
    21841 ttcctaaaag ccgtatcggt cattacgctt ttggaatcgt tactgctaca cgaaaagatt
    21901 taggtgcgtt tgtcgatatc ggtcttccag ataaagactt tgtcgtatct cttgatgaat
    21961 tgccgacaat gacagagcta tggcctaaaa aaggggatcg cttgatgatt tcccttagag
    22021 ttgatgcaaa agaccgtatt tggggcagtt tagcagatga aaaaatattc aaatctttaa
    22081 gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat aggaacagca taccgcttga
    22141 aattaacagg tacgtacgtt ttaacggaag atttctatct tgggtttatc catccttctg
    22201 aacgatataa tgaacctcgt ctaggcgagc agataaaagg tcgagtgatt ggtgtccgac
    22261 cagacggtgt actgaatctt tcattgaagc caagagcata tgaagctata tcagatgatg
    22321 cacaaatgat tttaaccatt ttagaacgtt ctgcagatca gcaaatcaac tttacagata
    22381 aatcggatcc agaagaaatc atgaaagctt ttggtatcag caaaggagca tttaagcgcg
    22441 cattaggtaa tcttttgaaa caaggattga ttacacaaga aaacggtgtg acaaaactag
    22501 tcaaaaaatc taattgagaa tcccttgcat gtttttctta actagcttat aataatttta
    22561 attaacatag tgttatatct ttttttattt gtaattatta taaattgttg gggttgagta
    22621 aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa cagcagctac atgaagcggg
    22681 gtttaagctg acaccgcaaa gagaagctac tctgcttgtg cttttggaaa atgagaaaga
    22741 gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa aagagtccgg aaattggact
    22801 tgctactgtt tatcgaaccc ttgaaatctt gacagaattg aaaattgtcg ataaagtaag
    22861 ttttaatgat ggattagccc gttatgattt gagaaaagaa ggcgccaaac atttccatca
    22921 tcatttacta tgcttgaact gcggcacgat cgaggaagta gaagaggatc ttttacttga
    22981 agtggagcaa gtagtagaag aacgttatca ttttttagta aaagatcatc ggctaacgtt
    23041 ccatggaatc tgtcaggaat gctatgaaaa agaaaa
//
LOCUS       DACDTO010000038        23065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDTO010000038 DACDTO010000000
VERSION     DACDTO010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.038"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IWK70_001505"
     CDS             complement(109..564)
                     /locus_tag="IWK70_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614038.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IWK70_001506"
     CDS             complement(718..1926)
                     /locus_tag="IWK70_001506"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9614039.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IWK70_001507"
     CDS             complement(2038..2370)
                     /locus_tag="IWK70_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9614040.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IWK70_001508"
     CDS             2518..3393
                     /locus_tag="IWK70_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9614041.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IWK70_001509"
     CDS             complement(3475..4056)
                     /locus_tag="IWK70_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9614042.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IWK70_001510"
     CDS             complement(4062..5321)
                     /locus_tag="IWK70_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9614043.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IWK70_001511"
     CDS             complement(5323..6369)
                     /locus_tag="IWK70_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614044.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IWK70_001512"
     CDS             complement(6604..6879)
                     /locus_tag="IWK70_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9614045.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IWK70_001513"
     CDS             complement(7122..8432)
                     /locus_tag="IWK70_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9614046.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWK70_001514"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWK70_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9614047.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IWK70_001515"
     CDS             complement(9978..10658)
                     /locus_tag="IWK70_001515"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9614048.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IWK70_001516"
     CDS             complement(10707..11342)
                     /locus_tag="IWK70_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9614049.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IWK70_001517"
     CDS             complement(11408..12811)
                     /locus_tag="IWK70_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9614050.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IWK70_001518"
     CDS             complement(12798..13829)
                     /locus_tag="IWK70_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614051.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IWK70_001519"
     CDS             13860..14081
                     /locus_tag="IWK70_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9614052.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IWK70_001520"
     CDS             complement(14246..14983)
                     /locus_tag="IWK70_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9614053.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IWK70_001521"
     CDS             complement(15136..16476)
                     /locus_tag="IWK70_001521"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9614054.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IWK70_001522"
     CDS             complement(16713..18236)
                     /locus_tag="IWK70_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9614055.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IWK70_001523"
     CDS             complement(18614..19225)
                     /locus_tag="IWK70_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9614056.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IWK70_001524"
     CDS             complement(19555..20271)
                     /locus_tag="IWK70_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9614057.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWK70_001525"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWK70_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9614058.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IWK70_001526"
     CDS             complement(20830..21627)
                     /locus_tag="IWK70_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9614059.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IWK70_001527"
     CDS             complement(21597..21968)
                     /locus_tag="IWK70_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9614060.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWK70_001528"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWK70_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9614061.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctagtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaa
//
LOCUS       DACDTO010000039        22026 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDTO010000039 DACDTO010000000
VERSION     DACDTO010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.039"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..1371)
                     /locus_tag="IWK70_001529"
     CDS             complement(61..1371)
                     /locus_tag="IWK70_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9614062.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IWK70_001530"
     CDS             complement(1383..2801)
                     /locus_tag="IWK70_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9614063.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IWK70_001531"
     CDS             complement(3041..4609)
                     /locus_tag="IWK70_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9614064.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IWK70_001532"
     CDS             complement(4613..4894)
                     /locus_tag="IWK70_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9614065.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IWK70_001533"
     CDS             complement(4891..5814)
                     /locus_tag="IWK70_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9614066.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IWK70_001534"
     CDS             complement(5811..6689)
                     /locus_tag="IWK70_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9614067.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IWK70_001535"
     CDS             complement(6676..8013)
                     /locus_tag="IWK70_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9614068.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IWK70_001536"
     CDS             complement(8016..9119)
                     /locus_tag="IWK70_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9614069.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IWK70_001537"
     CDS             complement(9193..10701)
                     /locus_tag="IWK70_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9614070.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IWK70_001538"
     CDS             complement(10667..11359)
                     /locus_tag="IWK70_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9614071.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IWK70_001539"
     CDS             complement(11381..12355)
                     /locus_tag="IWK70_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9614072.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IWK70_001540"
     CDS             12655..13527
                     /locus_tag="IWK70_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9614073.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IWK70_001541"
     CDS             complement(14109..16307)
                     /locus_tag="IWK70_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9614074.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IWK70_001542"
     CDS             16539..17531
                     /locus_tag="IWK70_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9614075.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IWK70_001543"
     CDS             17591..19012
                     /locus_tag="IWK70_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9614076.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IWK70_001544"
     CDS             19005..20504
                     /locus_tag="IWK70_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9614077.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IWK70_001545"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IWK70_001545"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9614078.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6669 a         4494 c         3823 g         7040 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaa
//
LOCUS       DACDTO010000040        21988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDTO010000040 DACDTO010000000
VERSION     DACDTO010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.040"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWK70_001546"
     CDS             <1..395
                     /locus_tag="IWK70_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614079.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="IWK70_001547"
     CDS             398..1147
                     /locus_tag="IWK70_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9614080.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="IWK70_001548"
     CDS             1159..1428
                     /locus_tag="IWK70_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614081.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1590..1892
                     /locus_tag="IWK70_001549"
     CDS             1590..1892
                     /locus_tag="IWK70_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614082.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="IWK70_001550"
     CDS             1889..2050
                     /locus_tag="IWK70_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9614083.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="IWK70_001551"
     CDS             2047..2352
                     /locus_tag="IWK70_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614084.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="IWK70_001552"
     CDS             2352..2708
                     /locus_tag="IWK70_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9614085.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2668..2913
                     /locus_tag="IWK70_001553"
     CDS             2668..2913
                     /locus_tag="IWK70_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614086.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="IWK70_001554"
     CDS             2910..3329
                     /locus_tag="IWK70_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9614087.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="IWK70_001555"
     CDS             3326..3883
                     /locus_tag="IWK70_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9614088.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="IWK70_001556"
     CDS             3880..4176
                     /locus_tag="IWK70_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614089.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="IWK70_001557"
     CDS             4253..4666
                     /locus_tag="IWK70_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9614090.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="IWK70_001558"
     CDS             5012..5386
                     /locus_tag="IWK70_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614091.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5616..5960
                     /locus_tag="IWK70_001559"
     CDS             5616..5960
                     /locus_tag="IWK70_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9614092.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="IWK70_001560"
     CDS             5965..6246
                     /locus_tag="IWK70_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614093.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="IWK70_001561"
     CDS             6349..6663
                     /locus_tag="IWK70_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9614094.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="IWK70_001562"
     CDS             6641..8335
                     /locus_tag="IWK70_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9614095.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="IWK70_001563"
     CDS             8355..9533
                     /locus_tag="IWK70_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9614096.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="IWK70_001564"
     CDS             9496..10182
                     /locus_tag="IWK70_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9614097.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="IWK70_001565"
     CDS             10182..11342
                     /locus_tag="IWK70_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9614098.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="IWK70_001566"
     CDS             11352..12227
                     /locus_tag="IWK70_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614099.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="IWK70_001567"
     CDS             12224..12535
                     /locus_tag="IWK70_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614100.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="IWK70_001568"
     CDS             12525..12878
                     /locus_tag="IWK70_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9614101.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="IWK70_001569"
     CDS             12868..13269
                     /locus_tag="IWK70_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614102.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="IWK70_001570"
     CDS             13262..13666
                     /locus_tag="IWK70_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614103.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="IWK70_001571"
     CDS             13678..14286
                     /locus_tag="IWK70_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9614104.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="IWK70_001572"
     CDS             14306..14668
                     /locus_tag="IWK70_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614105.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="IWK70_001573"
     CDS             14671..14853
                     /locus_tag="IWK70_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614106.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="IWK70_001574"
     CDS             14870..18289
                     /locus_tag="IWK70_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9614107.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="IWK70_001575"
     CDS             18340..19077
                     /locus_tag="IWK70_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9614108.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="IWK70_001576"
     CDS             19087..21378
                     /locus_tag="IWK70_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614109.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="IWK70_001577"
     CDS             21402..>21988
                     /locus_tag="IWK70_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614110.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DACDTO010000041        21912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDTO010000041 DACDTO010000000
VERSION     DACDTO010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.041"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWK70_001578"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWK70_001578"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9614111.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWK70_001579"
     CDS             complement(1172..1867)
                     /locus_tag="IWK70_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9614112.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWK70_001580"
     CDS             complement(1880..2596)
                     /locus_tag="IWK70_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9614113.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWK70_001581"
     CDS             2926..3534
                     /locus_tag="IWK70_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9614114.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWK70_001582"
     CDS             3859..4182
                     /locus_tag="IWK70_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9614115.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWK70_001583"
     CDS             4364..5734
                     /locus_tag="IWK70_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9614116.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWK70_001584"
     CDS             complement(5864..7165)
                     /locus_tag="IWK70_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9614117.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWK70_001585"
     CDS             complement(7519..9510)
                     /locus_tag="IWK70_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9614118.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFSNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWK70_001586"
     CDS             complement(9654..9920)
                     /locus_tag="IWK70_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9614119.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWK70_001587"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWK70_001587"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9614120.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWK70_001588"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWK70_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9614121.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWK70_001589"
     CDS             13264..13827
                     /locus_tag="IWK70_001589"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9614122.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWK70_001590"
     CDS             complement(14080..14601)
                     /locus_tag="IWK70_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9614123.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWK70_001591"
     CDS             complement(14604..15446)
                     /locus_tag="IWK70_001591"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9614124.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWK70_001592"
     CDS             complement(15439..16164)
                     /locus_tag="IWK70_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9614125.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWK70_001593"
     CDS             16340..16636
                     /locus_tag="IWK70_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614126.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWK70_001594"
     CDS             17204..18796
                     /locus_tag="IWK70_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9614127.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWK70_001595"
     CDS             18809..19549
                     /locus_tag="IWK70_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9614128.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWK70_001596"
     CDS             19717..21066
                     /locus_tag="IWK70_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9614129.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWK70_001597"
     CDS             complement(21194..21784)
                     /locus_tag="IWK70_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9614130.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4461 c         4158 g         6998 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattact gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DACDTO010000042        21504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDTO010000042 DACDTO010000000
VERSION     DACDTO010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.042"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(592..1983)
                     /locus_tag="IWK70_001598"
     CDS             complement(592..1983)
                     /locus_tag="IWK70_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9614131.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            complement(2196..2582)
                     /locus_tag="IWK70_001599"
     CDS             complement(2196..2582)
                     /locus_tag="IWK70_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9614132.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            complement(2638..3261)
                     /locus_tag="IWK70_001600"
     CDS             complement(2638..3261)
                     /locus_tag="IWK70_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9614133.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            complement(3325..3993)
                     /locus_tag="IWK70_001601"
     CDS             complement(3325..3993)
                     /locus_tag="IWK70_001601"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9614134.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            complement(4005..4445)
                     /locus_tag="IWK70_001602"
     CDS             complement(4005..4445)
                     /locus_tag="IWK70_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614135.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            complement(4504..5176)
                     /locus_tag="IWK70_001603"
                     /pseudo
     CDS             complement(4504..5176)
                     /locus_tag="IWK70_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(5361..5759)
                     /locus_tag="IWK70_001604"
     CDS             complement(5361..5759)
                     /locus_tag="IWK70_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9614136.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(5756..6529)
                     /locus_tag="IWK70_001605"
     CDS             complement(5756..6529)
                     /locus_tag="IWK70_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9614137.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(6526..7209)
                     /locus_tag="IWK70_001606"
     CDS             complement(6526..7209)
                     /locus_tag="IWK70_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9614138.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(7376..8263)
                     /locus_tag="IWK70_001607"
     CDS             complement(7376..8263)
                     /locus_tag="IWK70_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9614139.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(8260..9129)
                     /locus_tag="IWK70_001608"
     CDS             complement(8260..9129)
                     /locus_tag="IWK70_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9614140.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(9266..10552)
                     /locus_tag="IWK70_001609"
     CDS             complement(9266..10552)
                     /locus_tag="IWK70_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9614141.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            10825..11403
                     /locus_tag="IWK70_001610"
     CDS             10825..11403
                     /locus_tag="IWK70_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9614142.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            11403..12332
                     /locus_tag="IWK70_001611"
     CDS             11403..12332
                     /locus_tag="IWK70_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9614143.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            12329..14065
                     /locus_tag="IWK70_001612"
     CDS             12329..14065
                     /locus_tag="IWK70_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9614144.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(14128..14964)
                     /gene="pheA"
                     /locus_tag="IWK70_001613"
     CDS             complement(14128..14964)
                     /gene="pheA"
                     /locus_tag="IWK70_001613"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9614145.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(14961..15470)
                     /locus_tag="IWK70_001614"
     CDS             complement(14961..15470)
                     /locus_tag="IWK70_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9614146.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(15463..16758)
                     /gene="aroA"
                     /locus_tag="IWK70_001615"
     CDS             complement(15463..16758)
                     /gene="aroA"
                     /locus_tag="IWK70_001615"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9614147.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(16771..17862)
                     /locus_tag="IWK70_001616"
     CDS             complement(16771..17862)
                     /locus_tag="IWK70_001616"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9614148.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(17879..19045)
                     /gene="aroC"
                     /locus_tag="IWK70_001617"
     CDS             complement(17879..19045)
                     /gene="aroC"
                     /locus_tag="IWK70_001617"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9614149.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(19047..20111)
                     /gene="aroB"
                     /locus_tag="IWK70_001618"
     CDS             complement(19047..20111)
                     /gene="aroB"
                     /locus_tag="IWK70_001618"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9614150.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(20144..21166)
                     /gene="aroF"
                     /locus_tag="IWK70_001619"
     CDS             complement(20144..21166)
                     /gene="aroF"
                     /locus_tag="IWK70_001619"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9614151.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
BASE COUNT         6216 a         4703 c         3761 g         6824 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tacgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt tcttaaacgg tgttcaaaag
      361 cttgcatctg cggtaataag ctcaaccaaa cgtaaaatat gaggaactat ttttgtttgg
      421 ctgttcttat tacttgatgc agggcagctt tttcacaacc tcttattcac ggtgtttcag
      481 aagtagcttc tcggaaataa gccgaaattt cacaaaaatt tgagaaacta tttttaggaa
      541 acttcttctt atttcttaaa gctgaccact tctatcacaa cctctttttg actatgcatt
      601 cacatcgatc actttagaat catcattgat gacatcgcga tctaaatctt tgatgcgtgc
      661 acttgtcacg ataccagcta ccatgctacc gctgacgttt actgctgtac gtgccatatc
      721 gattaatggt tcaacagaga taactaaccc aacgattgct actggcaagt tcattgcccc
      781 taaaacgatc agtgaagcaa atgttgcgcc cccaccgact ccagctacac caaaagaact
      841 gattgttaca attgccacaa gcatcaaaat aaattccaaa ctaaagacat tcactccagc
      901 tgctggtgcg acgatcgttg ccaacatagc tggataaacg ccagcacagc cgttttgtcc
      961 aatagacaaa ccaaaacttg ctgagaaatt agctgttgca tcatctactc ctaaagcttt
     1021 tgtctgagtc tcgatattca atggcaaagc tccagcactt gaacgtgaag taaacgcaaa
     1081 gctcaatacc ggaaaggctt ttttgaaata gttgattgga ttgacttttg caccaatcaa
     1141 aatcagtgag tggacaatca aaacagcaag caatgctacg taagaagcaa cgacaaattt
     1201 acctaaattg acgatagctg caaaatcact tgttgccaaa acgtttgtca tcaaagcaaa
     1261 tactccataa ggtgtcaaac gcaaaacaag cgtcacgatc cgcataatga ttttatacag
     1321 actgtcgact aaatctgaaa agaatgctgc ttcttttggc gcttttcttt tcacgcctag
     1381 ataagcgatt ccgacaaatg ccgcgaaaat aacgactcca atcgtacttg ttgcacgact
     1441 acctgctaaa tcagcaaaaa cgtttttagg gataaaggat aaaatctgct gtggaatcga
     1501 taagttttca actgtttcct gacgtgttgc cagttcagag attcgtgctg tttctgcagc
     1561 tccttgtgtg aatttagctc cttggagtcc gaacagcatt gttgaaccaa ttccgatcaa
     1621 tgctgcaata gctgttgttc caagtaaaga agtcaataca gtcgcactaa ttttccctag
     1681 cttttcagaa actttcattt ttgtgaatgc accaatgatc gaaataaaca ctaaaggcat
     1741 aactagcatt tgcaagaatg atacatatcc ttgacctaca atcccgatcc attccattga
     1801 ttgagtaacg actttgtcgt ctacaccaaa gatcaattga agcagaccgc caaagacgat
     1861 accaactcct aatgctgtaa agacgcgtgt tgaaaatttt gtatggcgct tttgcatacg
     1921 atagaacaca tataataatg ccgcaaaaac tagaaccact aaaactgtga tgagtgttgt
     1981 catttttctc ctcctcatat tattaatgac aagtccatcg aaggaagtaa aggttcccga
     2041 gaatgattct tcaaccatcc tcctgaaaaa aattttttca gtgactttta tattttagac
     2101 gatttttatc gaaatgacat tctttttgct tacaattagt aataaaaaaa catacctgtg
     2161 atagctttaa tctatcgcag gtatgttttt actgtctatt cttgtttttc tgattgaaaa
     2221 ttcaatttta ccccaggctg atctaaggcg atctctgtca cctcataaga cattcggcgt
     2281 acgatatcga ataacggtgc tactagctcg tccacttctt cttgtgtgac atcattttga
     2341 ccttcaattt tccgattgag gatatgattg atctgactca cacttccaga taaaacatat
     2401 gtatcaaaca ctaaacggaa ctctaagcgt gctcctacga tgctatgttc tttcggatag
     2461 ttttcgatcg tttcttttaa aggcgagaag cctactttca aatctgtttc tacttcttgg
     2521 ttcggcaatc tcatatcata atgaaatgct tctacaactt ctttttgtct tctgatttcc
     2581 atataatttt atcactcctt tatgaaaatt ataacatatc tcctttgaaa ggggatacta
     2641 ctttttaatg atgctctgca aggtaaggat ttcattcaga tttcctacca cataatctgc
     2701 cgggatctca cccagaaaat gatcgatgtc gtaaagacaa gtagcgactc ctgcattttt
     2761 ccctgcttcg atatctaaac gacgatcgcc gatcatcatc gtgtctgttc tttctaaatg
     2821 aaaagtatcg atcaaataat ttaaagatgc tggatcaggt ttacgaggaa agtcctgatc
     2881 gatccctact acttcctcaa tcagatgtgc caatcgatgt tcttttaaca ggccccatgt
     2941 tgattctgtc aaacggtggg tcaggatgaa atgacgaccg ccgttgtctt ttagcatttc
     3001 cagtacttgt ttcgtttctg gaaatggacg ggaaacttcc gtactttctt tttcatatcg
     3061 gtgaaataat tcattaaact ctctggcatt caagtttgat tcattgatca aataactagt
     3121 agaatacttt ttcattttga agtagatttc ttttttgtcc atactgatac caaaatcttt
     3181 tagtgcttgc catgcaccat ctaccattgc gggatacgta tcaaacaatg taccatcaaa
     3241 gtcccatata taatttttca tgttcctttt gttccattcc tttgcttctt ttttctatct
     3301 ctcactttta gataaaggtg ttctttaaag tatttttttg tccagaattg tcaaatcatc
     3361 tgctaatgta tactggattg gttgtgcatt tgggatatcg atcgtatcca tttgatcttc
     3421 aggaacatct tctaaatatt ttgtcagtgc tcggatgcta ttaccatgac ccacaactag
     3481 taaatcttgc cgatctttta gttttggtgc tagatgatct tgccataagg gaatgatccg
     3541 attcactgtc atttccagac tttctccgta tgggatcaaa tgcggatcca agtgatcata
     3601 tctgcggtca aatctatttt ctgcggttaa cggcggaact tccgtatatc ctctgcgcca
     3661 tgtttttact tgatcttttc cgtatatttc tttcatctta tctttgtttt tacccacaag
     3721 atccccataa tgccgttcat ttagacgcca tgtttttatt ataggaagcc ataactgacc
     3781 catttcttct agtgcgactt gtccggtaag aatcgcacgt ttcaaaacag aggtataaac
     3841 ggcatcgaat tgaagtccgg cttcaagcat tttttgaccg gcaactgctg cttgttcttt
     3901 tcctttttct gttaaggcga catctagcca tcctgtccag taattttcaa aatttgcttc
     3961 gctttcgcca tgacgtaata atactaattt cattcttttc tcccttaaac attcgtttcg
     4021 aattttatac cacatgtttt gcatgtcaca cgtattcgtc cttttccttt aggtgcacgc
     4081 tgcttttgat gacaattcgg gcactcaaaa aaagtataac tttttcgatc cattttgact
     4141 tgctttttgt tttttatctg atgatatttt tctttgactc tatcaagata tttcaaataa
     4201 cgttgattct catttagtct ggggtagatt ttttttgata ggaaacgaaa tatcatccat
     4261 ccgaaaaaga cgatgaacaa caattgtccg attctatagg gcaaccaacg acttagcaaa
     4321 ctaaagacaa gcgcagcgat caatagaccg cgattcaact gatcaaattt cgcatatctt
     4381 ccctgcataa gtttgtgata gttctgctga aatcgtatta gcctttctaa ccatgtttgc
     4441 atcatattat ctcctccatc atctaatatt tattatgaca aaaaaatcca aaaagaaaaa
     4501 gccttaattg gccttttcca ttgcaacttg gtgaaggaca ttccgatttc tggtaaagtc
     4561 aatcacaatc actttctttt tatcttctct gttgactact cgaaatgtgt ccgtcaatgg
     4621 aagaaacagc ttcttagatg gtagctgttg catacgtata aaacgatgga tcatcgtaat
     4681 caattgagga aattgaatag acgtgtgaag cagacctgga tattttttca acagctgtgt
     4741 cgttttgatc agattccgtt tttttactga tcacttttaa aaagcttttt agaatctttt
     4801 gctgacgtct aaaaatagaa aaagaatttt cgtcatctat ttgataacgc atttgttttt
     4861 gcagttctgc tgcagtcaga tggatcttcc catcgtttgt atcgatcatt aaaccttctg
     4921 gaaacagttc aaccactgct cgatcccaag attcaagaga aagctcgata taatctttta
     4981 ctggtaattc caattctacc ttaaaaaagt gcaaaacagc cgacagccct tctgcttgat
     5041 agatttctcc taatgttagc ccctctagat ggaactctgg tgaaaaatgc agcaatgttt
     5101 tattttcctg caattgaaca tatagaaatg tagatcgctc aatcagagta tcttttaaaa
     5161 caactaaaaa attcattttt ctcccccttt tttcatcatt tttaaaatag catattttga
     5221 aaagaaagtc tgatgcttag agtagaattt cataaaaatt tactatttca taagtattca
     5281 cgagaaaaat acaaacaaaa aagcggaaat aacgctcatc cgccttcttg tttgtgttta
     5341 tttcctcttc aaacgcttgt ctatgattca gacttttctc tatcgttatt ttcatctaaa
     5401 agttcttgca ttccgataat ttctcgatat tgatgaatct tagtgatacc aatgactttg
     5461 tctacatcaa tactaaaaca aataccatta cctggaatat cgatgttgat gccttgacca
     5521 atcgcatcca ttactttatt cgtcaaacta tcgggtacta atgtcaacac aatatctttt
     5581 tctggcgaaa tgtccaaacc taaaaatttc cctgaatcat gtacaccaga acctcttcca
     5641 tgtaagatcg ttccgcctgc tgcacctgct tcttttgaaa agtcgataac atcttcgccc
     5701 attcctccgt caacgatcgt cacaatcatg tgaagattca atgctacaag atctttcatt
     5761 tgctttcccc caatttttgc aatctttctt tttcgattct gtctttttca tcttttcgtt
     5821 tataaatata tcctagtact aaagtggata atattggtgt caatgcaatc attgcaacca
     5881 caccaaaccc gtcaatgatc ggatctcttc cttcaatttg tgcagcgatt gccacgctca
     5941 ttgataaaat aaatgtcgaa cacatcgggc ccgttgctac tccaccgtta tcaaaagcca
     6001 ttgctaaaaa gatcttgtcc acttttcgtc ctaaaataaa tgctagcccg tatcctggga
     6061 tcaagaaata ataaagagag aatccgacga gcaaacgcca catagacaat cctactgctg
     6121 cgccgatccc aattgaaatt accatcagca tgattttcga tcggattctc ccctcgctca
     6181 attcttcaac ctgtttgatc attacatgaa tcgccggctc agcaaaccct actaagaacc
     6241 ccattaaaaa gccaagggga accagtatat aattgtaagg caatcccgca atagcactgc
     6301 ctaaatgctg tcctaccggt acataagcca tattgactcc gtgcaggaaa aggatcaacc
     6361 caatagtcgt tatcaagaaa cctttcataa tgtcaaagaa acgctttttg cgcatacgaa
     6421 aactaatgat attcattaaa acaaatagga ttacgatagg tgccactgcc aaaatgactt
     6481 ccatggtaac gatttcgatt ccatctatca cttgctgcca gaaactcatt ggaagatcac
     6541 ccccaaaatc atcacggcaa gaatcggccc taacgaagca ataccgacca ttccaaaact
     6601 gtcattttca tttttctttt gtttgatcat actagtcatc cctccggcaa gggccaagat
     6661 aaatggaaca gttactggac cagtagtcac tccccccgaa tcaaaagcaa tcggcataaa
     6721 ttcggaactg gtgaaaaatg aggcgatcaa aacgcctatg tagcctatag ccatcaattg
     6781 ataataggat agcttgaata cgacccgaag cagcgcaaag gctagaaata ctccagtgcc
     6841 gacgctgacg atcccaatca gcaacactct gccaatctct ccgtcagata tatcatatac
     6901 ttgttgtccc aaaacttgca cggaaggttc agcaatcgtg atcacgatcc cgatagcaaa
     6961 tcccaaactg accaaaacgg tcaaactttt ctttttgatc atatatttcc ctactagatc
     7021 tccgacttcc atcatagaat aatctgctcc aaacaaaaag agcgtcattc cgatcatcat
     7081 gattgccgca ccaaccagaa atgaagcgag gtcttctcct tctaacggtg caaaaacaaa
     7141 cgttaaaatg acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt
     7201 ttttagcatg tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg
     7261 agcacaaact taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa
     7321 aagtatcttt ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt
     7381 ttacagagcc gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag
     7441 ttggtaaagt agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag
     7501 cacccatagc agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta
     7561 agggccataa ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg
     7621 tatttttagc cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta
     7681 atcgagcggc ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga
     7741 tcaaaaaagc agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa
     7801 ccgttccgtt caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa
     7861 ccatcatcga tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct
     7921 caaatccata tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga
     7981 tcatcgctga attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa
     8041 aattgatgat caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat
     8101 tcgtcattcc caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta
     8161 atgctgtata ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga
     8221 cgttggggcg ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac
     8281 tttcgtttgg attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc
     8341 cgcatatcca aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt
     8401 ggtcgttgca tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa
     8461 tgtcccgatc gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt
     8521 cactttgata aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc
     8581 cgaatcaata ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca
     8641 agtgatcgaa agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg
     8701 tgtgtcaatc ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag
     8761 acttttcatg atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt
     8821 tcgaaacaac cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc
     8881 tagaaacaac atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt
     8941 tttaaaagtt gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt
     9001 gacaggggga ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat
     9061 gattgatatc aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt
     9121 ctgcttcatt cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag
     9181 acaatatctg taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg
     9241 aagaaattca cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg
     9301 tatctgctag taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta
     9361 cgactgcttc aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc
     9421 atgttgaaaa atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag
     9481 cttcttgttc attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc
     9541 ccatcagttc ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc
     9601 cagctactcc tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt
     9661 gcagattttc tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg
     9721 ctccttggat cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt
     9781 gtatcgcagt tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa
     9841 gagtggcaaa caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct
     9901 ccccatcttc agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa
     9961 ggtcagaagg attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta
    10021 cttgaatata ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg
    10081 ccatcagatc cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata
    10141 ttttatctcc tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa
    10201 tagcagacaa attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat
    10261 cttcaatcaa gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca
    10321 gtttctgaac gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct
    10381 cataaacttt ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga
    10441 cagtgacttt ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac
    10501 ctgatgctgc cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc
    10561 gctccttttc catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa
    10621 acggtttctt tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa
    10681 atcaactatg acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt
    10741 tctttaaaat aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag
    10801 actattctta aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag
    10861 acgattttag atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata
    10921 gggacttttt ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc
    10981 gtggtcacag atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa
    11041 cagcaacaac ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt
    11101 aaagattctt ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat
    11161 ttttttctaa attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc
    11221 ccaatcacca atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa
    11281 actcgtccat tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg
    11341 aaaaacggcc attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat
    11401 tgatggatag cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg
    11461 gctatgtaat caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc
    11521 aagaagtttt tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca
    11581 atgaaatatt ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa
    11641 agaaccttgc ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa
    11701 gtagttattt aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga
    11761 atttcttcac gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa
    11821 tcaatcgttt ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac
    11881 tttctttgct caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt
    11941 tttctattct tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa
    12001 aagacaattt cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag
    12061 aaatcaattt acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca
    12121 tctttactga aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta
    12181 aagccaagct ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg
    12241 gctataccga actgggttat ttctccagag tttttaaaga agagacagga atgacacctt
    12301 ctcaataccg taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt
    12361 tgccaaactt ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt
    12421 gtttatttat catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt
    12481 ggtactaaaa aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt
    12541 agccgctttt gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca
    12601 tatggaacag cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat
    12661 ccatccaatg atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg
    12721 aaacgacatg ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag
    12781 agtgaatgaa cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg
    12841 cgataagaaa gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt
    12901 ttacggatat gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt
    12961 agtaaatcct gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc
    13021 aaatgaagaa aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga
    13081 aacaacgatt tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac
    13141 attactcagt caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat
    13201 tattttgtat aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga
    13261 tcgacacaca aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg
    13321 cttaaaagct actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat
    13381 caatcaattg ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa
    13441 gaaacgttcg cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa
    13501 tacgctgact agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg
    13561 aatcgacagt ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc
    13621 agtcaaggaa gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg
    13681 cgaaagaatc tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa
    13741 tatgatcctt caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca
    13801 aggattcatc acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga
    13861 taacggcgat ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat
    13921 cggcatcagc catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac
    13981 gatccaatca gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa
    14041 aaatcaatca gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt
    14101 aaataaatca atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg
    14161 ataacagccc aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc
    14221 tactaatgtc attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct
    14281 agttttcaat ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc
    14341 cagcatttta tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt
    14401 tactggagct gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg
    14461 gttcaactca ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt
    14521 ttctaatgaa gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc
    14581 agcagttgtt gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg
    14641 ttgtgattgt gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt
    14701 gcctaaaagc tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg
    14761 tttggaaaga agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac
    14821 gacagccaga tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata
    14881 cgcagtcaat tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc
    14941 tggccctaga tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc
    15001 ttttctggtg tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa
    15061 agtcgttgtg ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga
    15121 atagacgttt catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa
    15181 agaacaatcg gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca
    15241 ccagtcgcga taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt
    15301 cgaaagcttt tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc
    15361 tcaatctttg tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg
    15421 attgttgttt ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc
    15481 tcctgacaca agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc
    15541 agctccttct agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc
    15601 gattcgatga tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat
    15661 gatcagaccg tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac
    15721 cgcatcaatt ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat
    15781 tccttctgct tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat
    15841 gatttctcca ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga
    15901 ttggttctgt tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc
    15961 taagatacca gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg
    16021 aacaagaagt cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat
    16081 ctgttgacca gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat
    16141 ccttccgcca aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac
    16201 gattacggat gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc
    16261 actggctaca ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat
    16321 tggtagatac tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc
    16381 catcactcgc cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa
    16441 aggctgtcca gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc
    16501 aattgattcg gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc
    16561 ttcctcttct tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc
    16621 ttctgcgcgc aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat
    16681 gctgcgatgg gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat
    16741 ttgttgtaat aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt
    16801 agacataatg attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag
    16861 tattcctaaa atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc
    16921 ttttccgatc agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa
    16981 taaatcaaaa aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc
    17041 ttttgcccga ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc
    17101 tatttgtgtc atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct
    17161 caacaaaata tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa
    17221 gcctcctgct gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt
    17281 gactaagcca gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg
    17341 ttctcttgct gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt
    17401 ttcaacatct tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag
    17461 tttagcatct gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac
    17521 aaaccgaaga tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc
    17581 cgtaatgatg acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt
    17641 tgtacggacg ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc
    17701 acttgcaacg taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact
    17761 atcccaacca actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc
    17821 accaatcagt ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt
    17881 agtaatgttt caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat
    17941 cacttgggaa ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa
    18001 cgacgctagc tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt
    18061 cttttgtatc gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga
    18121 ccccgcgaat gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat
    18181 tggtacgtcg gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc
    18241 ctggtaatga accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac
    18301 taaccacagc ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta
    18361 atccagctgg aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt
    18421 tctttgtctg atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta
    18481 cttctgacgc ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg
    18541 cttggattcc accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa
    18601 ttttcttggc aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt
    18661 caagcacatt ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc
    18721 ctggacgcgg acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag
    18781 acatgacagt tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac
    18841 caattgtttt gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca
    18901 tccttcctcc tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt
    18961 ctgacagagg taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg
    19021 attctcccgc agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct
    19081 gtgggaacag gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg
    19141 cttcctcttg ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga
    19201 tcgtacgtaa tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca
    19261 gttggagaca agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact
    19321 ccttctccat gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta
    19381 tgaccaaaat ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt
    19441 cgtttgattt ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg
    19501 tctttcagct cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg
    19561 atttcagcga ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca
    19621 atcaatacac cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg
    19681 actgctgtct tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga
    19741 acgaaatgga tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt
    19801 cctccaccaa gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg
    19861 tataactcag ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg
    19921 actggttcaa aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca
    19981 acgtttgtat cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat
    20041 ccagcctgtt ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt
    20101 acagtaagca ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa
    20161 tcttttccat tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt
    20221 cattcaacga ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga
    20281 tcatgccatc tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa
    20341 ttcctgtacc atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg
    20401 ggacagcact taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt
    20461 cgataaaaat gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt
    20521 cattgatcgt accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag
    20581 cagatagcag tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact
    20641 cagccaccat atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt
    20701 catcagccgc ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg
    20761 gagaagtacg tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca
    20821 ttcgcgcaca ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca
    20881 cgaaactgcc gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga
    20941 attctcgaga agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat
    21001 agcgtgtaaa tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc
    21061 gttctgttcc ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt
    21121 tttttatttg ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt
    21181 tcttttgttt acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat
    21241 taattcctac taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta
    21301 tgacccagat aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat
    21361 cttcaacgag ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct
    21421 ctacgtggct gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg
    21481 tttgtaccca cgaagtcatg aata
//
LOCUS       DACDTO010000043        21492 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDTO010000043 DACDTO010000000
VERSION     DACDTO010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.043"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            173..874
                     /locus_tag="IWK70_001620"
     CDS             173..874
                     /locus_tag="IWK70_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9614152.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1483..2499)
                     /gene="tsaD"
                     /locus_tag="IWK70_001621"
     CDS             complement(1483..2499)
                     /gene="tsaD"
                     /locus_tag="IWK70_001621"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9614153.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2496..2960)
                     /gene="rimI"
                     /locus_tag="IWK70_001622"
     CDS             complement(2496..2960)
                     /gene="rimI"
                     /locus_tag="IWK70_001622"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9614154.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2967..3509)
                     /gene="rimI"
                     /locus_tag="IWK70_001623"
     CDS             complement(2967..3509)
                     /gene="rimI"
                     /locus_tag="IWK70_001623"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9614155.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3493..4317)
                     /gene="tsaB"
                     /locus_tag="IWK70_001624"
     CDS             complement(3493..4317)
                     /gene="tsaB"
                     /locus_tag="IWK70_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9614156.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(4406..5386)
                     /locus_tag="IWK70_001625"
     CDS             complement(4406..5386)
                     /locus_tag="IWK70_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9614157.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5410..6894)
                     /gene="galT"
                     /locus_tag="IWK70_001626"
     CDS             complement(5410..6894)
                     /gene="galT"
                     /locus_tag="IWK70_001626"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9614158.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6906..7895)
                     /gene="galE"
                     /locus_tag="IWK70_001627"
     CDS             complement(6906..7895)
                     /gene="galE"
                     /locus_tag="IWK70_001627"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9614159.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8143..8310
                     /locus_tag="IWK70_001628"
     CDS             8143..8310
                     /locus_tag="IWK70_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9614160.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8372..10183)
                     /locus_tag="IWK70_001629"
     CDS             complement(8372..10183)
                     /locus_tag="IWK70_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9614161.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10180..10545)
                     /locus_tag="IWK70_001630"
     CDS             complement(10180..10545)
                     /locus_tag="IWK70_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9614162.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10708..11103)
                     /locus_tag="IWK70_001631"
     CDS             complement(10708..11103)
                     /locus_tag="IWK70_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9614163.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11121..12083)
                     /locus_tag="IWK70_001632"
     CDS             complement(11121..12083)
                     /locus_tag="IWK70_001632"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9614164.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(12083..12295)
                     /locus_tag="IWK70_001633"
     CDS             complement(12083..12295)
                     /locus_tag="IWK70_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9614165.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12316..13014)
                     /locus_tag="IWK70_001634"
     CDS             complement(12316..13014)
                     /locus_tag="IWK70_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9614166.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(13034..13576)
                     /locus_tag="IWK70_001635"
     CDS             complement(13034..13576)
                     /locus_tag="IWK70_001635"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9614167.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13708..14712)
                     /locus_tag="IWK70_001636"
     CDS             complement(13708..14712)
                     /locus_tag="IWK70_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9614168.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14709..15698)
                     /locus_tag="IWK70_001637"
     CDS             complement(14709..15698)
                     /locus_tag="IWK70_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9614169.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15695..16501)
                     /locus_tag="IWK70_001638"
     CDS             complement(15695..16501)
                     /locus_tag="IWK70_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9614170.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16667..17623
                     /locus_tag="IWK70_001639"
     CDS             16667..17623
                     /locus_tag="IWK70_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9614171.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17700..18218)
                     /locus_tag="IWK70_001640"
     CDS             complement(17700..18218)
                     /locus_tag="IWK70_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9614172.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18306..18455)
                     /gene="rpmG"
                     /locus_tag="IWK70_001641"
     CDS             complement(18306..18455)
                     /gene="rpmG"
                     /locus_tag="IWK70_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9614173.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18683..19129
                     /locus_tag="IWK70_001642"
     CDS             18683..19129
                     /locus_tag="IWK70_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9614174.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19324..21219)
                     /gene="asnB"
                     /locus_tag="IWK70_001643"
     CDS             complement(19324..21219)
                     /gene="asnB"
                     /locus_tag="IWK70_001643"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9614175.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6471 a         4559 c         3701 g         6761 t
ORIGIN      
        1 aatcattcgc ttcaaaacaa attattcaat accattgtat attttttaca taataacaaa
       61 ttatatttgt aaagtattaa aacttgtttc taatgttggt atgttaaaac tttacaaatt
      121 ctttaaaaaa agtgacaata aatatagtga acaataagga gaggactttt taatgcgtgt
      181 taataaaatc gattttcact tttcaaaact cagatatcaa cctgattttg aacatttctc
      241 ggatgaagag ttccaacaga tcaaggaaca tactgttctt cgttcttata aaaaaggaca
      301 gatccttttc gacgatggtg atcccagaca acggttgtat atcttgacca aaggactcgt
      361 tcgtttagaa cgttatgatg aaagtgcaac ttattactac tatgactatg tgaatacaaa
      421 taaccttttt cctatgtcag gaatgtttgg agaagagacc tacacttata cagctcaagc
      481 gatgactgat attgaaacat tctaccttcc tgtttcctac tatgaaaaat tagcacgaaa
      541 aaatcccgaa cagttgatct atatgataca aaaaatttct aaagtagttg aaatacatga
      601 attgcgtatc caaatcggat tgacctctag cgcctttgac cgggtaaaac aaaatttatt
      661 cattttgaaa gaagaattgg gcgtaccaac aaaagatggt catatcacca ttccttatcc
      721 gatcacctta aaagaattag cagtgaatag cggcacgaca cgtgaaactg ctggacaagt
      781 aatcaaacaa ctacgtgatt ccgggctgtt agattatgac aaaaagcgtt ttactttctg
      841 taagacagct actactccag ctatgagaga ataacgcgaa caaaaataag cagtgaccct
      901 gataaatatc agggtcactg cttattttcc tccctaaaac ttctgaaaga tccggcagtt
      961 ttagatcact ggacaagact caaagatttt ctatgtgtgt ttcagttatg taatattcac
     1021 tgtttgcaaa aaatactgtt agattaacta gatgaagcgt cttgtcctca tccgatctag
     1081 attattcgat attcctcgac aataaacgat tactcagtga gggcaatcag aggaacggat
     1141 aatgataaat aagctgtaaa aaatagcaaa ataacaataa tgattaaaag tatgtaaaaa
     1201 atagggcttt gacaaaaacg tgtcacagcc ctatttcgat taaacggtgt gtacctgtca
     1261 actcttcggt cacaagtcat aatgttcata aacatgagga gctgtttttt gaaattgttc
     1321 cttgtgaatc agagttaaac gacaggctta cgaactcttg attaaacggc gttcaaaagc
     1381 ttgcatacgc ggtaataaga acagccaggt caaaaacatg agaagccgtt tttcctgggc
     1441 tgttcttatt acttgatgca gggcagcttt ttcacaacct ctttattcag ataaaaccaa
     1501 gcttggatca gcattcaact gatagctagc aaaatttctt ttttgcattt ccacatgtgc
     1561 tgctgctcca atcattgcag cattatctcc acaaagtcga agagggggaa tcactagttc
     1621 tacctcaggg agtttagctg ccaaagcatc ctgcaatcct tcacgcaacc cttgattggc
     1681 cgctacacct ccagctataa tcagttgctt gactggataa ttttgacaag ctcgaagtgt
     1741 tttagtaatc aatacatcga tcacacttgc ttgaaagcta gctgctaaat catttttgtc
     1801 tagatcttct ccacgctgtt gtgcattatg caccaaattg ataaatgcac tttttaatcc
     1861 gctaaaactg aaatcataat tatcttcatg gatcatcgct cgcggaaaat ggtaattatc
     1921 ttttccctga tgagctagct gatcgatttc ttttccgctt gggtaagaaa gacctaatac
     1981 tcttccaact ttgtcataag cttctcctgc tgcgtcatct cttgtctccc caatgatttc
     2041 atatgatcca tcttcttgca tatagactaa ttctgtatgt cctccgctaa caagaagtgc
     2101 catcaatgga aactggaatg gtttaaccag acgggctgca tagatatgcc ctgccatatg
     2161 gtttacagga ataagcggca attggtgtgc ccaagcaaaa gccttagctg cagaaatacc
     2221 aatcagcaac gagccgacaa gtcctggtcc ataagtgaca gcgactgccg ataaatcttc
     2281 tgccgacaca ccagcttcta ccagtgcatc ttccaagcat aaggtgattt gttctacgtg
     2341 atggcggcta gctacttcag gtactacgcc accaaaacgt ttatgactgt ttatttgaga
     2401 agcaacgatg ttagataaga tctctgttcc atttctaacg accgctacac tcgtttcatc
     2461 acaacttgat tcaatcgcca gaatcaattc ttcattcatt tcatcgtctc cgtttttaac
     2521 tttctgctca taatgatcgc atcttccgtc ggattgcgat aatatgattt ccgtttacct
     2581 aatatacgaa acttttctga ttcataaagt tttcttgctg cttcattaga tagacgtact
     2641 tctaaaaaaa catgcagcga atcttttatt tgttccgtaa tcagtaattg taacaaatgc
     2701 cgtgcatgcc cttttccttg ttctgaaact gcaacagcaa tatttgtgat ctcgacttca
     2761 tctattactt tcgagtatcc aataaagcca cagatctggt tatcttcagc taaaagcaga
     2821 taatcagagt gaggctgttg gatgtctgcc aaaaactgca ttttcgtcca aggtgaacca
     2881 tgtggataag cattttcgct aatcatccat acgcattctg ccaagtacgc ttcatcaaaa
     2941 tctttttttc tgcaaatcaa ctaatctcac tcattttcta aaagttttat catatccaac
     3001 gcatcttctt ttgtttcagt gtaatatctt tttttgatcg ttgttgcttg aaaccccaat
     3061 ttccgataaa gtctttgtgc atcttgatta ctcatacgca cttctaatga catcgtctca
     3121 cagcgattca ttatagcaaa gttctcgatt tcgtcaataa gcagcgaacc gattcgttta
     3181 cgctgatatg ccggtcgaac ggcaatattc gtgatatggc aatctaaacc gaacgctctt
     3241 gaaccgataa aaccaatcat tcttccatta tcaaagatac ctagatatag atgaggaagc
     3301 ggtgacttca attctgatat aaaggcactt tttgtccatg gcaattcgcc attatagact
     3361 tctcgttcga catccagcag ctctttgata tcttctgtag tcaattctct tagtacgtaa
     3421 cgacgatcga tttgttcgac tgttttttct gtataaatca ttttatcttg aaaaaagctt
     3481 ctggcatacg ctctaaattt tttcaacata actttcgtct ccaggtgtgt gcgtctccaa
     3541 ccatttttct tctgcttcta ctcgttttaa atagcgagga agaaaagctt gtttctctgc
     3601 aactggttca actaaagcac ctaattgagc cacagttaca ccacttggaa tatcccatgc
     3661 tggaactgta ttaatcatcg cactaggcaa tatttccttt atttcagaga agaatttgtg
     3721 acaatcactc ccgacgaaat aaatagcttc attcagcttt tgtagttgtg tgaacaaatc
     3781 agaagcagaa atatgtacat cttcaagtac agtttctaat ctattttctt tccagcggta
     3841 tgccccagcg tatacatttt ttcttcgagc atcaaacatg ggcacaataa tattatccac
     3901 accgatgcaa ttagccgcga tggttttcag actagatact cctactagct ctttatctaa
     3961 tgtataagca atcgtttttg ctgtcgttac acctatacga agccctgtat aagaaccggg
     4021 accatcagaa accgcgattc ggtcaatgtc ttttggactt atttgaagat ctgcaaacaa
     4081 ttggtcgatt gcaggcatca gtgttacact gtggttgcgt ttgatatttg tttgtatttg
     4141 tccaagaatt tgttcgtctt ctaccacacc gatcgccaaa gtttggtttg cagtatctat
     4201 tcctaatgta atcatatatt tgatcctttc tgctatgaca ttctcgcctt ccattgtagc
     4261 atactttttt attctgagga tgaaaatacc aaaggcaaaa ctagtacaat aatacatgat
     4321 tatctgctgg cgggcaaatt ttgccccttc ctgaaacaca aaaaagctgc ggtatcactc
     4381 cattttccca cagcttttcc atcttttatt tcgttgattc tcgaataatc aactctgttt
     4441 ccacaataat ctttcttgaa accggtgctt tggaattact taattgaaga atcgtctcca
     4501 ctgctagctc tcccatccat tcggtataaa cttttacagt agaaagcgag ggcgttacat
     4561 atttcgctac actgacatca ttgaagccca tcacagaaat atcttctggt accactaaac
     4621 ctttttcttg aatcgctttc agtgcgccaa tcgctaatgc atcattagct gcaaaaagcg
     4681 catcaggaac atgcggattc gtttttaaat attgcttcac tgcttgatat cctgactcga
     4741 cagaaaaatc tgcttcgata attggacctt cgtcgtagcc tttttctgcc aaaacttgtt
     4801 gaaatgccat aattctggga tcttttagcc gctgatggtt tttcttcgta tattctgttc
     4861 cagcaagcat agcgatatgt tgcgctttat gggagacaaa gtgttcaatg actttttgaa
     4921 cacttgactt aaagtcgaca actagagagt cgattccttg agaagtgccg tcagaatcta
     4981 ccaaaagcaa ggcattttgg gatcctttca tttctaataa ctgagcttcg tcgaattttc
     5041 ctagtgcgat cgttccatct gccggaagat cactaatgga atcccacgtt tctttcaata
     5101 aagtaacgtt tgcttcttct gccttttttt cgattcctag gcgaatagat aaataataaa
     5161 gatcttcaag ctcttccgta tcgtcgtacc actggatcag gcgaagcgtt tgcttagtat
     5221 ttgctgcatt tttacgatgt ttcgtataat ttaagcgttc tgctgcttca aaaatttttt
     5281 gctttgtggc taagccaacg gaaagatcag gatcatagtt gagaactctt gaaacagtag
     5341 caggagaaac tcctgccaac tgcgcaatgt ctttaattgt tgccatttgt ctctcctcct
     5401 tttttacgtt tataaaatcg cagtaaatcg ttcaaacgct gcctgtcctt tttcatctcg
     5461 cttgaatact ccagcgtctt ccagtacacg tgcaaaaacc tgacctacag atttttgtac
     5521 gacatcggtg accgtatctt tcgtcaaatc aggatgttct ttttttaatt gttctgccca
     5581 cggacgatga tattcagcca tctggttttc ttctcctaat aagaaatgct ctacttctac
     5641 tagttctggt ttcaaacgag gtggtaaaac agcaagcccc attacttcga tcaacccaat
     5701 gttctccttt ttgatatgtt ggacatcttt atgtggatga aaaatcccat caggatgttc
     5761 ttctgaaaca ttgttgtctc gaaggacaag gtccaactca aagtaattgt cccttcttcg
     5821 tgcaattggc gtaatcgtat ggtgtggtgt accatctgct gaaaaagctc tgatagatac
     5881 tgtttcatcc gaatatgctt tccatttttc caatactgag ttagctgctt caataagcaa
     5941 gtttttctct gttgattgca aacggatcac agacatcggc catttcacga ctcctgcgat
     6001 gacttgcgga taatcttcta gtttgataag tttttctatc ggtgcttttg ccataggaaa
     6061 ttcatgacgt cctgcctgat aatgatcatg agaaagaatc gatcctccaa caattggtaa
     6121 gtcagcattc gaaccaacaa agtaatgagg caagacttca gtaatccgaa gcagtctagc
     6181 aaatgtttcc tttgtgattt tcatcgggcg gtgttgttct gataaaatga tcgaatgttc
     6241 attgtaataa gcataagggg aatattggaa tccccagctt tcaccgtcta gattcattcg
     6301 aataatccgg tgattggtgc gggctggata gttcaagcga cctctatatc cttcgttttc
     6361 catgcaaagc atgcaggtag gatagtctac ttttaccgcc tcgcgttctg ctgcgatttg
     6421 ttttggatct ttttctggct tagataaatt gattgtgatt tccagttcac catactccga
     6481 ctctgccgga aatttgatgt tttttgcgat ggctcttgtt ttgatgtagt catttttctt
     6541 acttaaatta aagaaatact cagttgcttc ctctgggctt ttcgaatagt gctgtgcaaa
     6601 gaatgcattg acgactgaag gtggcggtgt gagaaaatcc attaattgcg cttcaaaaat
     6661 ctcttgttct gctggcaatt ctgaaagaac accattttct cttgccttag cgacaagctg
     6721 atccaataag tctacagatt cttccttaac tggtcttact tcaatgcttt ctagagcatc
     6781 ttctcctacc atccctaaaa gacgattctg taaatataag cggtccattt ccatccatcc
     6841 gccagcttcg atcgcaagtg tggcaaaatc acaaatcgtt tgactgattg acatatctca
     6901 ttcccctatt cttcataacc atttggatgg ctgacatgcc aatcccatgc tgttttgata
     6961 atcgctttga tatctgtgta ttcaggtttc cagcccagaa tctctctcgc tttttcactt
     7021 gaagcgacta atcggctagg atctccagct cttcttggtg ctatttctgc tggaatttct
     7081 tttcctgtca cttctctagc tgcttctagc atttccttga ctgagtatcc tttgctgctg
     7141 cctaaattga agaagttgct ttcatttcct tctttcaagt attccaaagc taaaatatgt
     7201 gcagcaatca aatcttctac ttgaacataa tctctgatac atgttccatc tggtgtatca
     7261 taatcgtctc cataaacagc caaggctttt ctttgaccaa gagctacttg taaaatgatt
     7321 gggacaagat gcgtttctgg tgtatgatct tctccgatag atgcatctgc ttttgctccg
     7381 gctacattga agtagcgcaa agcgacatag cgcatgccat atgcttggtc acaccacttc
     7441 atcatttttt ccatcatcaa tttactttca ccatacggat ttttaggatt agctggtgta
     7501 tcttctgtga ttggagactc tttcggctct ccataagttg ctgcagtaga agagaatacg
     7561 attttattca cgttgaactc gtgcatgact tcaagtaaga tttgcatacc gtatacgttg
     7621 ttgttgaaat acatcaatgg tttttcgaca gattctccta ctaaagagct tgcagcaaaa
     7681 tggattactc cttcaatcgg ttccttttca aatacggaac gtagaaattc tttatcccgg
     7741 atgtctcctt cataaaaatg agcatctgga tgtactgctt gtttgtgacc tgtgagcaaa
     7801 ttatctacca cagctacttg ataccccttt tgaactagct ggtctacagc gtgcgaaccg
     7861 atataaccgg ctccgcctag tactaaaatt gtcataaaaa acactccttt gattagtaaa
     7921 attgtgtttg ttgattctat ataatttaaa aaacgaagtt tacttttatg gtgaaaacaa
     7981 acaaagtaaa atttcgtaaa ttcatatggt aaatttataa taaaacattt agtaaacatt
     8041 ttcaagggtt ttttgcaaag gtttacaaac aaacgtaaaa ctagcctttt tttctctttt
     8101 gttccataat agatataaca aataataaat gaggtggatt caatgaaaaa atttgtatct
     8161 ggaatattag taggaagtct ggcaacagcc gctgccgttg ctggtttggt tgcttctgtg
     8221 aaaaaaacag tgatcgatcc aattgacgaa aaagaagcaa tgatcgaaga aaaccgtaaa
     8281 aaagcaatgc gtaaacgcgt ttctcgttaa taattattta aaaatacgca tgaagacttt
     8341 ctaaaaaaca gataagtcat catgcgtatt tttatttgtc agtcaatata agttgtgcta
     8401 ttttatctgc ctgtctcaga ttccatggat ctgtataagg tctagctact gctgcagtta
     8461 gccaatccac accttcaaca ggtattccat agcaataaaa gtcagactgg atttcccctg
     8521 aagcatcaat cacgcgattc gtacctcgct caacatctat agcttctgaa tgataacttc
     8581 ctgtattatc tgaaaatgaa aaagggcgaa caagtttgtt ttccttcaaa ttttttatga
     8641 caggattact tgaacgagaa aagcttgttt gtggcaatcg agcttcaatc aaatagcgac
     8701 tgctgatttc tttatcttgt agagaaataa catagcggtt gtgcttttgc tgaatatcaa
     8761 atggcggttc gatcaaatga aaaatacccg catctatcag tgctgctaat tctttcgtcc
     8821 gaatgattgg cggaccgatc gttaaaaagc tatttagatg agtaaaccat ccccatagta
     8881 tttctttgta ttccttagca gaaaaaacgt gccattcaat agcctgccgg attggatcac
     8941 gccagtcttt taatgcatca aatgttgaag caaatgctcc tgtgcaattg cctttttccg
     9001 cttcttttat ctgcccttca ataaatattc ggctagcttc tgaaaagggc tcattctgat
     9061 aggcaagagg agtttctaaa tacgaccaat cccatctata agggattagt gctggatgct
     9121 ttccgagaca ctcctctgaa gagagggaga gaaaatcatg ctcaaaattt ttctttggaa
     9181 tcggcaagtg atattcttcg atgattttct tgtaataaaa caactcaacg tcttttttca
     9241 gatagtcaaa aaatgtttcg ccagtcaaaa atcccgactg atgccattgc tccaggtttt
     9301 cttttgttag taaccgtggc catgccattg ctccgccttg cttttgattt cttcctcgag
     9361 ggtaataagg gagccccctt cttgatccac tgtatacaat cggttccttg cctgatggat
     9421 gatagaccag ttttcccttt tcgttctcga agcgccctcc tcttgatact gtaaacaatc
     9481 cgacataatc aaaaaaactt aagcctagac ctcttaaaaa gacattttct ttaggcggta
     9541 tcttcgaaag atctacatct gcaggattag aaggaagctg atacagtaaa acatttttct
     9601 ttgcgtagtc tacccacact ctttcttctt ctgtctcatc attttcccag tgaccgcttg
     9661 ctaaaacaag ctgatcgata gaatagcttt gctgatctgt ctgtaaaaga taaccatctt
     9721 ttcttttgct tacttttttt actaaagaat aaacaaactg aatagaagct ggaaattctt
     9781 cttgcaactt tgagaaaaac cagcgttgat agagtccgta taaacatcga gtagattgct
     9841 gatctttttc cagacgattg atttcttcta taattagttg cttatttttg gttaccgtct
     9901 gttcgatata atctgtagca ggtcctttac tccattgaaa caagttgggt ccctgtgtta
     9961 ccaatcctcc actggacaat gtttcatctg taaaaagcgt cacttgttga gaaacagaat
    10021 tcattaataa ctccggtggc tgattcaacc gccaaactcg acctccagga ccattgggat
    10081 cgaaaagaaa aagttcaaga gaatgtctgt cctgattatt atggagaatc ctttctgcta
    10141 cacttaatcc tctaggtccg gcaccaatga tccctatttt catttgcatg cctccatttc
    10201 gataaatttt ttcaataaaa cagcatggtt ctgatgttca ttttttgcac cataaacaaa
    10261 agtaatgact ggatgttctt tcataagttg actgagttta aaaatagctt gcttcttttc
    10321 ttcttcgaaa atcaattctt gctggtattt ctttgcaaat gaagcaaatc gttctggtac
    10381 atggtcaaaa gttttgcgtg cttcattact aggtgcaatt tctttcaacc acagatctaa
    10441 ctgcgctttt tcttttgaaa ttcctcgtgg ccagaatcta tccacgagta ttctatagcc
    10501 atcctctttt tcagcatcaa ggtaaacacg ttttaatttg atcatagttg tttccttctt
    10561 tctacctttt cgcttagctt atatatgtag ataatagcac aaaataaaaa aaatgaacgg
    10621 cacaactatc cattgcgccg ttcatgtgat cttggtcaaa atctaatgat tgattcggtt
    10681 tttaaagctg tcatacaatc atctgtttta tactttttta cttttgattt ttccagacca
    10741 tccgtcatat ccacccttaa gtaaataaat ctcttgataa cctgctttac gtaatttatt
    10801 tgctgtacgg atgctcaaac ttttcttttg atcgtaaata tagacaggct ggtcttttcg
    10861 aatagatcct aatgaatctt tcaaaacagt gtaagggatg tttcttgcac ctaaaatatg
    10921 cccagcatca aacacatctt tttcgcgaac atcgattact tgtgcttttc tcattccttc
    10981 tcgaaattct tcttctgtca aagtcgttgc tgaacgtttg gccattactc gtagatagac
    11041 ttcccaaagg atgattgcaa gcacaatcaa tagcaaaatg atattaatta cccataaaac
    11101 catgtaatgc ctccgttttt ttaagcaaat ttcaaggcta atgaggcagc accaatgaca
    11161 ccagcttcat taccaagctc tgctaatttg atttttgttg attctcttac ttgcgggaaa
    11221 gtaaattctt taaaatattt ctctacacgg ctacgtaaaa attctcctgc tgccgatact
    11281 cctccaccta acacgatact tgacggattt aatgtattcc ccaagtttcc gcaagctaaa
    11341 cctaagtaga agcatacgcg atcaacaacc atcaatgcaa acggatcgtc ttcttgagcc
    11401 atttcaaaga tatctttact tgttacttct tctccgttgt ctagcattgc tttcagtttt
    11461 gaatcccctg cgtattcttc tgccaagaaa cgacctaggc gtaccacacc tgtagcgcta
    11521 gatacagttt ctaaacatcc gcgtttaccg caagtacatt cgaacccttc aggatccaca
    11581 gtgatatgtc cgatttctcc tccagctccg ctcacgccgt gaatcaaatt tccgccagca
    11641 acgatacctc cgccaacacc tgttcctaga gtaacgaata caacgtctgg atcgttttct
    11701 ccagcacctt tccatcgttc acctaaagca gcaacattcg cgtcattatc gatagcgaat
    11761 ttgattcctg ttcctgcctc gatcagtttt ttcacaaatt gaacttctgt ccagtttaag
    11821 ttatacgcac caataaccgt tccagcttcg ctgtccacgc ttcctggggt acccatgccg
    11881 attccgataa agtcttcggc tttcatatta tataaattca aacgatgatt gattgattca
    11941 atgatttcag gaacgatatg cttaccatca tccaaaatat tggtatcgat gctccatttt
    12001 tgctgaatct ctccttctgg tgttaaaatt gcaaattttg ctgttgttcc acctaaatca
    12061 atcccaataa tcttcttgtc cattacaaat ttcctttctt tcggctttct tcaattcggt
    12121 gctctctttt taatatgcgc cacgctgtca tgtatgtatc atgatcaatc aaacggctat
    12181 catacagatt acgcaattcg atagtcatta attcaatgtc atagatgcgt gcacccacat
    12241 agataaagat gccaaatttt ttgaacagct gctgtacatc atataagctc tccatcttta
    12301 agcacttcct tcatattata caagtaaacc atattttttt aaacccagta aaatacatat
    12361 tacaaaaaga aatacaaaaa ctagtgtaga aagaatcctt tggtgtatcg aatagcttgt
    12421 ctgttgtttc ggtagaccca aaacattccc taacactaat ccgccaatta gcccaccgac
    12481 atgtccccaa acatctacag aggtatccag aagaccaaaa acaaatgtca acacaataaa
    12541 taaaagaaaa tgtctgacta gctgctggat cgctgggtta tgtttaaaat gaaaccctaa
    12601 gataaacaac gcaccgaaca aaccgaatat cgaagtactt gcaccaccag acagaacatt
    12661 cgcttcgtta aaagcaaagc ttgctgtatt tcccattatc ccgctgaaca tataaatcaa
    12721 gaaaaaacgc caatggccat agattgcctc gatttgctgc cccataaaat acagtatcag
    12781 tgagttcatt acgaaatgca tcagtccaaa atggatgaag attggtgtca ctaaacgcca
    12841 ccattcattg aagtgaacaa gataaggacc atacattccg gcataataag ggatctcaag
    12901 tcgcggaaat aattccatca tgaggaagac cgctgtctgt atccctaaaa aagcataggt
    12961 ccataacggt cgtcttaacc atgcacgcaa ttttatttgc tgttgataat tcatgaaaac
    13021 ctcccatttc attttagtca ataaacaatt gttggatctt ctgatcaaag ctctccggtt
    13081 gccattgttc attcaattgt tcacgaaaaa caaggctaca tgttttttta tgaaaattat
    13141 ttaaataccg gtcgtaataa ccaccgccaa aaccgattcg ataaccggct ctattgtaga
    13201 taatacctgg tacaatgagg agatcgatca tctccggcaa atagagcttc tctgattgtg
    13261 gctcaagaac accaaaactc gaacgtacaa gaacggactg ttcatccatt tcataaaacg
    13321 ccatctgacg tttcggcaaa gttttaggta caactatttt cttttgttct ttttctgctc
    13381 gttggataac cggctgtgta tccagttcaa attcattcga taaagtcgtc cctatcactt
    13441 ttgctgactt ccagtactcg ctggcgaata gctgtttata gattcgctgt tctttttcct
    13501 ttttcttgcc acttttggcc agatttttca gcttttcgat tcctttccgg cgtaatctta
    13561 ctttcaattg gtccaaaaac tcacccttcc ttctctcata aaatggtata ggaaaaaaag
    13621 aaaaatagca atcgaacttt caaataaatg cataaataga ataaaaagaa gacctagaaa
    13681 tgattggcct tcctttttta atgaatttca actgttcagc gcgtgttttt gtctgataaa
    13741 aagtgcatac agaaaatatg gcgcaccaaa aagggcaatc aaaattcctg taggtacttc
    13801 cccacttggt agaatcactc tagcaaccat atctgcacta agcagaaaaa tggaaccgaa
    13861 caaaccgctc agaacaaagg tccagttatt ttctttacgt acgactagct tcgcagcatg
    13921 aggagctagt agtcctacaa aagataaact acctgtcacc gctacactac cgcttgccaa
    13981 agcaacagct aacaacaaaa ggattttttg acttttagcc acgttaaggc ctaatccttg
    14041 cgcgcgttct tgaccaaatg atagcaatac aatcctctgt gcataaagtc ctcctagcgg
    14101 aattaagata accgcccatg gaagcaatgc ccaaacataa ggccagctcg caccccagat
    14161 tgatcccgct aaccatgcat tcacaaatcc ataattttcc ttagataatt ggatcgtgca
    14221 tagcaatgtt aatgcagaaa ggccggcatt aacggcaata ccggacaata agattcgatt
    14281 catacccaaa aagccatttc gatcataggc cgcaagatag acacagcaag cagctaaaac
    14341 accacctaga aaggctacaa ctagcaatag aaacggcagc gaatagtgcg aaaagaaacc
    14401 taaataaagc atcactaaaa atcctgctcc ggcattaatg cccaatatac tagaatctgc
    14461 caattcattt cttgttactc cttgcaagat ataaccagaa acgccaagac aagctcctgc
    14521 taataaactg atcagtaatc gaggaactcg aaattctcta acaatcagcg ccaaagaaga
    14581 tgattctttc cctatgaacg tggcaaaaag ctgatccagg taaataaaag gcgtaccaat
    14641 cattaaacta cctatagaca ggatgacaag tacaaaaagc aagaaataga acgtccaata
    14701 tatttttttc accgttgttc cctccttact tgtatcagta aaaacggaac gccaatcaat
    14761 gcaatgacta atccaaaagg tgtttcaaaa gggggattga tcatcctcgc tgtaaaatcc
    14821 gccaacataa ccaatccacc gccaactaaa aaagagacag gtagaacata acgatagtcc
    14881 gttccaacca ttccgcgaac aactggcgga accaacagcc caataaatga aacaggtccg
    14941 actaacgaaa cggcaatggc tgctaacact gcaactagac agaaaacaat caaccgcgtt
    15001 cgtttaggat gacttccaag agctaatgca tgtgtttcac tcaaatgaat caaagatatt
    15061 gcttttccta atgaaaaaga aaccaaaaca gcaagtatat ataaaggaaa caatactgct
    15121 aattgtttcc atgtgatatt ggcagctccg cctacaaacc aaaaagcaag atcctgctgc
    15181 agattaaaga attgggtaag tgcttggctg atagcagtaa aaaaagaact aatagctacg
    15241 ccagctaaaa tcagctgtaa aggattcatg cccattttcg aaaactgcaa aaaaccaaat
    15301 acgagcaaag ttacagcaaa agaacctgcc aatgacatta gaaacacaga aaaatcagaa
    15361 gctttaggcc aaaaagcaaa actaagggcc attgccaacg aagcacccgc gttgattcct
    15421 agtaagcctg aatctgctaa aggatttctt gtcacccctt gcatcaatgc accgctcaat
    15481 gcaaaacaac tccctacaag aaatgctccc gccacacgag gcaaacgaat atttcgtatg
    15541 atttggtgac tttgttttga tctatcaaaa tcaaacaacg cctgccaaaa atctgctttt
    15601 tccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa taatcaaaag aacaaaacag
    15661 accaaaagca ggagtctatt tttcttcata gtcctcataa ttgatcctcc tgctcttttg
    15721 gcaacaaatc ataggttaga aaaactggtt tatttgttct tggaagcgtg acaatcgttg
    15781 catcaatctc aaatattttt ttcatacact cttttgtgat cacttcttca ggtgtacctt
    15841 ctgtgatcaa cttaccgttt ttcattgcta ataaataatc tgaaaaacgt gaagcatgat
    15901 taatgtcatg aatcgacatc aagatcgttg tttgtttcat ttgattgatt tcagtcaata
    15961 actgtaggat ctctaattgg tgtgccggat ctaaaaatgt tgtcggttca tccaagatca
    16021 agatatctgt ttcctgagca agcgccatag cgatccagac acgctgggtt tgtccaccag
    16081 acagcgttgc gatatcccgc atacgaaatc cagctagacc tgtagcatcc aacgcccact
    16141 caattgcttc atgatcttgc gtgtccatgc cactgaacag tttttgataa gggtatcgtc
    16201 cgtaagatac tacttcttct actgtcatac ctgtagggtg ttcactagtt tgtgctaata
    16261 atgcaatttt ttttgcaagt tcttttgaac tgtatgtaga gatatcagag ttattgacaa
    16321 tgatctttcc tgatttcaca ggtagtatac gggtaaaact tttcaataaa gtagattttc
    16381 cacttccatt tggcccaatc aaactagtga ttttcccttc aggaatcgta acagacatgt
    16441 tttcaatgat ttttttgttt tcgtagccgg ctgcaatgtc agctgctctt aatctcgtca
    16501 tttcacccat cctttcgctg aaaatcattc tcaattatga cgctgtttca acatttcgtc
    16561 aagcgctact ttcatatcta tagccaaatg aagcggttga cagcgttcga aatatcagga
    16621 ataataacca atgagaaaga ttctcaatta tgttaggagg aaagaaatga aattaaaaac
    16681 aaaagtattc acattatcta gcatattgct gctggctgga gtactgagcg cttgctcccc
    16741 ctcttcagat aaagaaacag ctacaactgc caattcgacg acttcatcca atagtactca
    16801 tacagtcaca gatacacttg gacataaagt agagattcca aacgcgccca aacggatcat
    16861 cggcagttac ttagaagact atctgattgc actaggggaa aaacctatcg cccagtggac
    16921 tgttggcagt ggttccattc aagattatct gcaagatgat ttaaaggatg tcccaacgat
    16981 ttcttatgac cttccttatg agaaagtgtt aagctttgag cctgatttgc tgttgatttc
    17041 ttcttccgct actgtcgaag gcgggaaata cgaacaatat agcaaaatcg cgccaactta
    17101 tgttgtaaaa aacggaacag atgtcacatg ggaagaacaa ctaaaagatg tcggcaaagc
    17161 tttggcaaaa gaaaaagagg cagaaaaaat catttccgat tatcaaaaat ccatcaagaa
    17221 aactcgagaa gaactagcag ataaaatcga gaacaagaca gctgcagtgt tgtgggtgac
    17281 caacaactca gcctttatgg tcagcaaaaa tcggtcaagt ggaagaatcg tatacgacga
    17341 tctggaattt ggtgttcctg atctagtgga agaggtctcc aaaaaagcca cttctgactg
    17401 gtctgctgtt tcctctgaaa agctagctga attagatgct gactacatca tccttgtgaa
    17461 cagtgacaaa gatgctgcca tgttcaatga accaagctgg aagaatctaa aagcagtgac
    17521 agacaatcat gtactgcaat tcggacctga atctagttgg ctatataacg gacctattgc
    17581 cagtcaggaa atggtcaatg atatcaaaga aaaactaaat tgattttaac taaaaaaagc
    17641 atcttcacga ttagtcgtga gatgcttttt ttcttattaa caaactagac gcatagattt
    17701 tattcttctt ttgaagcaac cctatttttt actctttccc atttttgaaa acgatgggca
    17761 tatagatttt cttgatccgt tatcccctca tactcctcta taagggcgaa gtctgaaaaa
    17821 tcaatattcc caatatacgt atcgccttca aacgtgctat cgatcaatgt tcgccagatc
    17881 cgaccagtgt aaggaagaag ttcttgaaaa atccgactac cacccgaaac atagatatct
    17941 tctgagatcg tttgactata cgacaaaagt tcatcgatcg aatgcattac ttccgcattt
    18001 tctttcgtgc gaaaatttgg ttgcgttgtc aacacgatga tatggcgata aggaagtgaa
    18061 agatccccca ttccttcata ggtctttctt cccatcacta aaattcgatc cattgtatgt
    18121 tcgcgaaaga aacgcatatc atttggcaat cgccatggta acaaaccatt tttgccgatc
    18181 agaccatttt tatcttgtgc ccacatggaa ataaacatat tatccgctcc ttatgatttt
    18241 agatgttttg agataaaaaa agtctcaatt ctttgaccag caaggaattg agaactttaa
    18301 aaacattatt ttgtttcacg gtgcaaagta actttgcgtt cgcgtgggca atatttttgt
    18361 ttttccaaac gatcaggatt gttacgtttg tttttgcttg ttagatagtt acgttcttta
    18421 caagaagtac actctaaagt gatgtttacg cgcatgtttt tccctcccat ttttacaaga
    18481 atcctctaag atgactctca gacttgatta tcttaccatg attttttcta gattgctagt
    18541 gtttttcacg attttgttaa gtaaaactac ttgacgataa atacaacggt tttacttctc
    18601 aaacaaaatc tttttctttt tgtataaata cccttacact tgtttatata gattatgttt
    18661 tttaataaag ggggatacta atatgccatg gaacatgaag gactttcctg catcaatgaa
    18721 aaacttagat aaattgacaa gaaaaaaagc aatcgacatc gcaaatgcat tattagatga
    18781 agggtaccct gataaccgtg cgatcccaat tgccattgat caagcaaaaa aatggtatga
    18841 agatgctagt gaatcagaaa aacgaacttt cgaaaaagaa aaaaatccaa gcaagacgga
    18901 tgaacacgat acgaatcctc gtgctggaaa actgcttgat tcagatgtga tcgtagaata
    18961 tgaggaagaa caatggatcg tgaaatctaa aggggcaaaa aaggcaagca accattttga
    19021 tacaaaaaaa gaagcaatcg aaaaaggaaa gcaagtagca caaaacaaag aatcaacttt
    19081 gttcatttat aaaaaagatg ggacaaaaga aaaagaaatc gattattgaa catatgtaaa
    19141 ggggttgtga cgaaaatcgt gtcacaaccc ccttatgttc gaaacaatgg tgttcctctg
    19201 ccaactccga accataagtc ataatatcta taaaacggga agagctgttt tttgaaattg
    19261 tttcttatca ctcagtgtta aacggtagga gaaaacctct tttttattga tatttgattt
    19321 tcatcaaaca gtcacatagt cgattgcttc tgcttgcgga atcgtttcat ctacaaagta
    19381 gactttatac catgtcaaga aactgaagat tgtccagatt tttctttgct cttctttacg
    19441 tccttcgtga tggtcatcta gcaacttcat gattttttct tgatcaaaaa attcttttgc
    19501 aaaatctgct tcgaataatg ctttgacttg ttcgtagccg tgttcttctt tcaaccaagc
    19561 tttgattggc acagggaatc ctaatttcac acgattcgac cattcttccg gaagatgttt
    19621 gttcgcagct tgtctgaaaa tatcttttgt gttgttttga ttcaataaaa acttacttgg
    19681 tatttgttga gccaatttca tgacttcgat atccaataaa ggtactcgga cttccaaaga
    19741 acttgccata gaaagtttat ctgcctttaa caagatatct tttggcatcc attgatgaag
    19801 atcgacatat tgcatcttat tgacttcatc tttgattcct tctgttttct cgtaatgaac
    19861 agtcatgatt tcatccacag atggtgctgt ttgatatttt ggtgttaaca cttctgatgc
    19921 ttctgattct tcgaacactc gagcatgacc gataaaatat tcttttgcag gagcaagtga
    19981 ttcatataaa tggatccgtc catggaagtt tttcattttt ccgattttac gtcctaaatt
    20041 atagcgtgtt ccttttggta attttttcaa tccttgagca atcacacgga tgaattttga
    20101 attcgtatgg aatccatacg tttggtatcc cgcaaacagt tcatccgccc cttctccaga
    20161 ttggactacc cgcacacttt gtgaagcaag atttgctaaa aagtacaaag gtacgcaaga
    20221 aggatttgaa tcaggttcat ctaagtgata ctggatcaaa gggaatgctt tgaacgacat
    20281 gtctccgtcg atcactgctg ctgtattatc taagtccaat agttcggtta gttttctggc
    20341 ttcaatcgct tcattatagg ttttatcatc aaacccgatt gagaaagaat gatctggttt
    20401 caatacagag gttacataac tggaatcaac cccgctggat aagaaagaac caacttctac
    20461 atcactgacc gtatgtgctt caatagaagc ttgaacggtt tcgtcgattt tttcgatcca
    20521 ttcttgtcgg gaatgcgttt cttttgtttc aaaatctgca tcccaatatt gctgaatgtc
    20581 tatttttcca tctttatacg tataataatg gccttcaggc aaacgataga catctttgaa
    20641 gaatgtttcg ccgtttaatg gagaatattg gaaagtcata taaggtttca acgcttcttt
    20701 gttcaattct ttgttaaaat caggatgagg cagaaaactt ttgatttctg aaccaaacat
    20761 aaatgttccg ttcatttctg cataataata tggtttgatc ccaaagtggt ctcttgcacc
    20821 gaataactca ttcttttcat tatcccaaat agcaaaagca aacattccgc ggatcttttg
    20881 caataattct gttccccatt cttcgtaacc atgcaaaagg acttctgtat ctgcatgtgt
    20941 ttgaaaaacg tggccagctg caatcaattc ttctctcaaa ggctggtaat tatagatttc
    21001 gccattaaaa ataatgatct ttgtccgatc ttcattgtaa ataggctgag taccgccctc
    21061 taagtcaata atacttaatc ttctgaatcc taaagcaaca tgtttatcga tatattctcc
    21121 tgaactatta ggtccacggt gaacgattcg atccatcatt gtattaataa ttgttttttt
    21181 gttgtccttg tcgttaacaa aacctacaat accgcacatt taactttcac atcctaatat
    21241 aaaattactg tttatttcat tcatatgcat cttatcatag aataaatagc ggacgaatat
    21301 tttctctctt acaatttcgt cacgtgaaaa caaaagatga aagtttctgt ttttacagaa
    21361 aataaagtcc caatatctaa tttaccatgt tttctttttt gaggctacct tattctcttc
    21421 gtgttaaaaa aatattagtg gatgctattt ccaaaaaaac cagcaaaaaa agacacgacc
    21481 taaagatcgt gt
//
LOCUS       DACDTO010000044        21432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDTO010000044 DACDTO010000000
VERSION     DACDTO010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.044"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..766
                     /locus_tag="IWK70_001644"
     CDS             <1..766
                     /locus_tag="IWK70_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9614176.1"
                     /translation="TANTVIHYHAFIRKALQEAVITELISTNVADKVRKPKKKQFVSQ
                     VYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGLKWSAIDFLNKKIMINHVII
                     ENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAKWQQDNRILLGDDYILEDNE
                     YVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRHSNASIMLDSGQNMKSIQEW
                     LGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            1340..1909
                     /locus_tag="IWK70_001645"
     CDS             1340..1909
                     /locus_tag="IWK70_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9614177.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1896..2756
                     /locus_tag="IWK70_001646"
     CDS             1896..2756
                     /locus_tag="IWK70_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9614178.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2946..3650
                     /locus_tag="IWK70_001647"
     CDS             2946..3650
                     /locus_tag="IWK70_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614179.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3655..5490
                     /gene="walK"
                     /locus_tag="IWK70_001648"
     CDS             3655..5490
                     /gene="walK"
                     /locus_tag="IWK70_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9614180.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5487..6803
                     /locus_tag="IWK70_001649"
     CDS             5487..6803
                     /locus_tag="IWK70_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614181.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6804..7673
                     /locus_tag="IWK70_001650"
     CDS             6804..7673
                     /locus_tag="IWK70_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614182.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7728..8537
                     /locus_tag="IWK70_001651"
     CDS             7728..8537
                     /locus_tag="IWK70_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9614183.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8640..9269)
                     /locus_tag="IWK70_001652"
     CDS             complement(8640..9269)
                     /locus_tag="IWK70_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP9614184.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9439..10731
                     /locus_tag="IWK70_001653"
     CDS             9439..10731
                     /locus_tag="IWK70_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9614185.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10807..11469)
                     /locus_tag="IWK70_001654"
     CDS             complement(10807..11469)
                     /locus_tag="IWK70_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9614186.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11694..11978
                     /locus_tag="IWK70_001655"
     CDS             11694..11978
                     /locus_tag="IWK70_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9614187.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            12029..13654
                     /gene="groL"
                     /locus_tag="IWK70_001656"
     CDS             12029..13654
                     /gene="groL"
                     /locus_tag="IWK70_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9614188.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(13743..14891)
                     /locus_tag="IWK70_001657"
     CDS             complement(13743..14891)
                     /locus_tag="IWK70_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9614189.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14993..15577)
                     /locus_tag="IWK70_001658"
     CDS             complement(14993..15577)
                     /locus_tag="IWK70_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9614190.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(15704..16135)
                     /locus_tag="IWK70_001659"
     CDS             complement(15704..16135)
                     /locus_tag="IWK70_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9614191.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(16153..16473)
                     /locus_tag="IWK70_001660"
     CDS             complement(16153..16473)
                     /locus_tag="IWK70_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9614192.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            17211..17468
                     /locus_tag="IWK70_001661"
     CDS             17211..17468
                     /locus_tag="IWK70_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP9614193.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(17439..17747)
                     /locus_tag="IWK70_001662"
     CDS             complement(17439..17747)
                     /locus_tag="IWK70_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614194.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            17825..18571
                     /locus_tag="IWK70_001663"
     CDS             17825..18571
                     /locus_tag="IWK70_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9614195.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            18586..18789
                     /locus_tag="IWK70_001664"
     CDS             18586..18789
                     /locus_tag="IWK70_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9614196.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            18894..19193
                     /locus_tag="IWK70_001665"
     CDS             18894..19193
                     /locus_tag="IWK70_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614197.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            19193..19375
                     /locus_tag="IWK70_001666"
     CDS             19193..19375
                     /locus_tag="IWK70_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614198.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(19381..19857)
                     /locus_tag="IWK70_001667"
     CDS             complement(19381..19857)
                     /locus_tag="IWK70_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614199.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            19910..20635
                     /locus_tag="IWK70_001668"
     CDS             19910..20635
                     /locus_tag="IWK70_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9614200.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLP
                     ETAIRMEIDQLNRQQRLFGDENE"
     gene            20809..21150
                     /locus_tag="IWK70_001669"
     CDS             20809..21150
                     /locus_tag="IWK70_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614201.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            21143..>21432
                     /locus_tag="IWK70_001670"
     CDS             21143..>21432
                     /locus_tag="IWK70_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9614202.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT         7250 a         3592 c         4307 g         6283 t
ORIGIN      
        1 tacagcaaat acggttattc attatcacgc atttatacgc aaagctcttc aagaggctgt
       61 tattacagaa ttgataagta caaatgtagc agataaagta agaaagccaa aaaagaaaca
      121 atttgtttct caagtttata atcaacaaga gatcttgaaa ttacttgaca ttttgtcaga
      181 agaaaaactc tatttggtag ttcttttaac agcattttat ggattgcgaa gaagtgaagt
      241 tttaggatta aaatggtctg ctattgattt tttaaacaaa aaaatcatga taaaccatgt
      301 aatcatagaa aatccagaga atatgtcaca gttaataaag aaagatcaaa caaagaactc
      361 ttccagctat cgtacattac ctttggttga aacgattgaa cgagcattag taaatcaagc
      421 taaatggcaa caagataatc gtattctttt aggagatgat tatattttag aagataatga
      481 gtacgtattt actatggaag atggacggtt gatgaagcct caatatgtaa cgcatcgttt
      541 gagtaagttg attaagaaaa atggattgaa aaaaattcgc tttcatgact tgcgtcattc
      601 taatgcttca ataatgttag atagtggcca gaatatgaaa tccattcaag aatggctggg
      661 acatgcatcg tattcgacaa ctgctaattt atacacgcat ttaaccgcag gagtaaaaga
      721 tcgtgcagca gaagcattag aaaacgcatt tgggtttaaa gaataaaaaa acaaaaaaca
      781 atatctttgg ggtatcgctt cttgttttta gaattttttt gttggcaatt tgttggcagt
      841 atgttggcaa tgaaaaaatt cacaaactaa aaacctattt ataaagcttt ataaaacttt
      901 tttcacaaat agagcatttg gttaagagtc agctgctcta ccaactgagc taacggcccg
      961 tacaaaaagt acttaataat agtagcacta agaattttaa aatgcaagac tacatttaaa
     1021 gaaaatcaga aaaatcttta cttccttata ggaaatcatt catagattat tcacatttgc
     1081 tcgctattat ataagcatac caacaaacaa agttcttgca agagaacaaa ccctaacact
     1141 tttttcattt tttactcctt tcccgccgtc tccccggcgg gtctcctgtt cttaacgata
     1201 aaagacaaga aggcttagcc ttcttgtttt ttgttttcat ggaatcccgc taatcaaatc
     1261 acctagttaa agcaactaga ttgaatgttt atcataagtc tgatatcatg tttatgaaat
     1321 gaaaaaagga gcggttatga tggaaaaaat gcgtcagcaa ttaatggagt gggggaagga
     1381 actagaaaca ttccgtctgc ctcactggga agaactccct gacttagaac tttatatgga
     1441 ccaagtcaga acgttagttg atcgttatct ttcacctgtg atacaaggag aaaagcatcc
     1501 attgcttacc tcttctatgg taaacaatta cgttaaacta ggtttgatac cagcaccagt
     1561 taaaaaaaga tataacaaag aacacgtagc atttttactt gcaatcacta cgttgaaaca
     1621 agtattgact attcctgaaa tcaaagaagg gatattgttc caaggaaaaa cagtaggaat
     1681 tcgtgaagcc tataatctat tttgcgatga acaagaagca gctgtgtgga tggtcagcca
     1741 attagcacaa ggtaaatctc atccgcaaaa attcgaccag gcaacccctg tagaatatat
     1801 tgctgtaaga gcggcaactt tatcgtttgc aatgaaactt cttgcggaaa aaacgattgt
     1861 tctagaaacc gaatatttaa aagaggagaa aacaaatgaa aaacaataaa atcgcaattt
     1921 tagtagattc aggtacggat gttccagaag aattgataaa aaaatacgag atgtatatga
     1981 tcccattgaa aattatttat aaagatcagg tatatacaga taaaattgac attacccctg
     2041 aagagatcta tcaacgtttg ccagaggaaa tccctggtac atctttgccg gatggtgagt
     2101 cgatcacaga aatctttgag caaatcaaaa aagacggtta tgaaaaggta cttgcggtaa
     2161 caatttccag tggattgagc gggacatata atgtggttcg tttaattgca caacagcaag
     2221 aagaattgga agcatatgta ctagatacga aaaatattgg tattggtgca ggtttttctg
     2281 cgatccaagc agcaaaatgg ctggaagaag gagtagaatg ggatcaactg atcagtaatc
     2341 tgaatgagtt agtcgaaaga acaaaagttt tctttaatgt tgcgacattg gaatatcttc
     2401 aaaaaggtgg acggatcggt ttagttgctt ctattgtagg aaccgcttta aagcttaacc
     2461 caatcatctc atgtaatgaa gaagggattt attacacagt aggaaaagct aggggacgta
     2521 aaaaaagttt ggataaaacg gttgcttacg tcaaagaaaa aatcggtaat gcaaaggtct
     2581 tcaacttagc tgttgcccat ggggatgcga aagaagaagc aattgaaatg gaagcgagac
     2641 ttaaaagaga gttcccacaa gcaaaccaaa tctactttgg ccagatttct ccggcattag
     2701 tagtccacac tggtccggga ttattgggtg tcggtgtaca agttttagaa ggataaatca
     2761 aaaaaaggaa acactgaggt gaaaatcagt gttttccttt tttattgaag ttagaacaaa
     2821 aaataataaa gtattttgca ctttcatttt aattttctct tttgttttat aggcaaagat
     2881 gaaacaaaag cagaaatacg gtataatgag aaggtatgac aataatatat aagaggtggc
     2941 tctttatgaa aaaagtttta gttgtcgatg atgagaagcc gatttcggat atcgtcaaat
     3001 tcaatttagc caaagaaggc tatgacgttt atactgcata tgatggggaa gaagccttgg
     3061 aaaaagttgc tgaggtagaa cctgatttga ttttacttga tttgatgcta cctaaaatgg
     3121 acggcttaga agttgctcgt gaggtacgca aaacttatga tatgccgatc attatggtaa
     3181 cagccaaaga ttctgaaatt gataaagtcc taggcttaga gttaggcgcc gatgattacg
     3241 tgaccaagcc attttccaac cgagaattag ttgcacgtgt caaagcaaat cttcgtcgcg
     3301 gtgccacggc tgcgaaggaa ccagaagagg cagcacctgc ggaattgacg attggtgatt
     3361 tgacgatcca tccagaagct tacatggtga cgaaacgagg agaaacaatc gagttaacac
     3421 atcgtgaatt cgaactactc ttttacttgg cgaagcacct tggacaagtg atgacaagag
     3481 aacatttatt acaaacggtt tggggttatg attattttgg agatgtacga acagtggatg
     3541 taactgtacg tcgtttgcgt gaaaagatcg aagataatcc aagccatcca aattatttag
     3601 tgacacgtcg tggtgtcggt tactatttgc gtaacccaga acaggagtaa ttggatgaaa
     3661 agaaaagtcc gcttttttcg atcagtcaat tttaagatcg ctattacctt tattctgatt
     3721 ttattgatct cgatcgaaat tattggtgcc tactttatac gtggtttaga acgttccacg
     3781 atcaatacat ttattaaaga tatgaatcaa acggtggaat cattagcaac aacaatcagc
     3841 ccggaattga atcgaaaaga taatgcagat gatgaagagg taaatgcgaa tatcaaaaga
     3901 tttattgaaa acagtgcgac tagcgatatc atcgaaatac gggtcgttga cgaaaaaggg
     3961 atcatccgag gaacaaccga tgtcaatgaa caaagcgctg tgggaaagaa aaatgattat
     4021 gttgatatca atgatttcac gactaaacgt tatgttgctt tagataacga cgataaacgg
     4081 gtgaatatca atgttcaacc gatcctgtcc ccgaccggcg atgccgttat cggggctctt
     4141 tacgtcaaaa gtaatatcga acaaaaatat tcggaaatca ataacacagc agtcattttc
     4201 tttactgcct cactaattgc ggcatttatt tcaatgatcg tatctgtttt agttgctcgt
     4261 tcgattacac agccgatcgg tgaaatgaga gaacaagcca ttcgaatcgc tagaggagac
     4321 tacagtcgga aagtcaaagt gcatggacaa gatgaacttg gacagctggc tgataccttc
     4381 aatcagttag ctgaacgtat tgaagaagct caagatacaa tggaagctga acgcaatcgt
     4441 ttggatagcg tgctatccca tatgacagac ggcgtcattg cgacagatcg caggggtaaa
     4501 gtcattacga tcaatgatat ggctgtttcc ttgctagata tcaaaaacga ggaagcaatc
     4561 ggacaatcga ttttgacact tttagatatc gaagaagaat atacgttgcg gaaattatta
     4621 gaagatccaa acgaaatggt acttgaccgt tcaaccagta cattggaaag tgatcagatg
     4681 atcttgcgag tagacttttc tatgatccgt cgcgaatcag gttttatcag cggattagta
     4741 gctgtcttgc atgatgtgac agaacaagaa aaaacagaac gggaacgtcg tgaattcgtc
     4801 tctaatgttt cccatgaact tcgtacaccg ttgacaagta tgcgtagtta tatcgaagca
     4861 ttgagcgaag gtgcgtggaa agacgaggaa gtagcaccaa attttttgaa ggtaacgcta
     4921 gatgaaacag accgaatgat tcgaatgatc aatgacctct taaatctatc tcgcatggat
     4981 acaggtaata cccagcttca attagaatat gtcaatttca acgagttagt gaattttgtt
     5041 cttgaccggt ttgatatgat ggtgggaaat caggaaaaaa attataagat ccgtcgtgaa
     5101 tttacgcaaa gagatctttg ggtcgaagta gatacagata aaatcatcca agtagtcgat
     5161 aatatcatga ataacgcgat caagtattca ccagatgggg gaacgattac ttgtcgctta
     5221 ttagaaacgc ataataacgt gattttaagt atcaccgatc aaggattggg gattcctaaa
     5281 aaagatctga accgagtgtt cgagcggttc tatcgagtag ataaagcacg tgcaagagca
     5341 caaggtggga caggacttgg attagctatt tctagagaag tgattaaggc gcatcgaggc
     5401 gctatttggg cagagagcaa ggaaggaaaa gggtcgactt tttatatctc gctgccatat
     5461 gaaccttatg aggaggaatg gtgggaatga gaatctctga aaaagtcgtt cgtataggac
     5521 tcattttgat ggttgcgtta agcatatact tctcttatgc aatctggctg agcccagcag
     5581 gaaaaagttc aatcaacgta gatgagacaa acactcaaat gatcgagtct caaagcaatc
     5641 gaaaaacttc cgaagtcttt cttccgcttc atgttacatg gcaccattcg ggaatgatcc
     5701 aagaaaccaa cagtgaaaat ctcgtgtacc gattgcaatt gatcatcgat aaagcaagat
     5761 ttggaaagct ttctgaagta gtttcaggag acaaatcaca gtttgaacag gtgaaaaatc
     5821 tgaactctgg ttttgagttg gcctataatg cgcctttttt gctaagagaa tacaaggaag
     5881 catttgattt agatttagat gtgtcgaact tggcaaaaga aagcgaaaat atgtatttta
     5941 ctagaataca attcgatcaa ggaaataaaa aaatccgatt tttgaattat tcgaaccatc
     6001 tcgtatatga agcaaatatt tcgttgaatt ggacagatat caataaagaa ttaacagcaa
     6061 aagacacaaa atggacagaa atgacaacag aaccatcgac gatcgacacg caatatgcta
     6121 cgaaacattc gatcaaactg aagaagtata gttatatcct gtcacagcaa ccctatacac
     6181 tttttagaaa tgcttttttc cagaaaccag atagcgtgaa aaataatgat gaaagcaatg
     6241 aattgatttt ttacggtggg aatgaaacaa tgtctatcca ttcggaaacg caagtagttg
     6301 attttcgtgg agagctgcca gagaaaaaag aaaatgctag tgtattttct gaaagtttcc
     6361 cctatattag caaattaggc agttctttag ggaacctcag atattttgac cgcagtgaca
     6421 atacgatcga ttatcggata ttcgtggaag gcttcccggt ttttgggaca gacagcagag
     6481 gacagattgg tgtggaagtt ggcggtgaaa cgaatggttc tgtgcaagtg aatatccaaa
     6541 ccagtttgaa tacgattcaa gtgccgatac cttcagacga agaagtagaa cttgcaagta
     6601 cgaatgaaat catccagcag ttaaaagcta agggagcaga tagcaacaag attggttcag
     6661 tgatcgttgg ctatacatgg caaaatgtca aagaaacgaa tcgtgtcgtt gatctcttac
     6721 ctgaatggta tgtgaaatat aaagataact ggtattcagc taacgacctt ctggaaaaat
     6781 tacctggatt ggaggcgaat taattggatt tcaaaaaaat cgaatggata tttttcgtcg
     6841 cttttttagg gttgaatgtg tttttattca gcagttatca tgacgcggta taccaagaaa
     6901 acaatgtcat tcgctctaat cagactgaat caatccaaca acgtctggct agcgacgata
     6961 tcacttacac ggggaagatt tcgtctgaga acaagcaagg ctattatctt agcgcggagc
     7021 agaccaatct tagcacttca ttagcgaatt atcgcaaggc tggtaaaagc ggcttgctgg
     7081 caagtggtgt gtttttagac ggtgatgttt tgacgaaatc tttatccagt tcagatgctg
     7141 aaaagaacgt gatcgatcca gataaaatta gtagttcctt ggaagatttt ttatctaaca
     7201 aagacaatat tttattcggt aatgattatg tgtacacgaa aaacttttct catttagaag
     7261 aagaaccact agaaatccaa gcaagccaag agtatgaagg catcccattc aatgatgata
     7321 cagcaaaaat cgttctttct ttagaaaaaa acgataatga ttatagcatc acgaaataca
     7381 tgcagacgca tttatctgat atcgagcagc tgcgcgaaaa gacggaactg tatacggaag
     7441 aggaagcaat caatacattg tacatcaata acaaaatacc aagaggatca aaaattttat
     7501 ggcgtcagct agcttattcc tgtattctga aagtccggga aaaaaatgtg tatgttcctg
     7561 tttggcatgt agcgatcgaa acaagcgata aagtggtcca aattgagagc gtcaacgctt
     7621 tcagcaatac gatcgtgaca aacaatactg taccaaaagt ggaagatcaa taaaaaagcg
     7681 agtataatga aaaaagttta caaagtaaga agggaagtag gagtcaaatg gctgaagaaa
     7741 aaccagcttt taaaatcagt atcctagcaa gtggaagttc gggtaattca ctttatatcg
     7801 aaagtgaaaa aaaacggctt ttggtcgatg caggacttag cggtaaaaaa atcacttcct
     7861 tgatggctca aattgatcgg acaccggacc aattagatgg aatacttgtg acgcatgaac
     7921 atcgagatca tatacatggc gtgggtgtct tggctcgtaa gtatcatttg gatgtttttg
     7981 ccaatcagca gacttgggaa gcgatggaac cactgattgg aaatgtacca gtggaacaaa
     8041 aacatctatt tgagatgggg aaagtcagaa cattcggcga tatcgatatt gagagtttcg
     8101 gcgtctccca tgacgcagcg gctccgcaat tttatcgttt ccataaagaa aatcggtcat
     8161 tcgtcatgtt gacggacacc ggatattgca gtgattatat gcgaggtgtc attgaaaatg
     8221 ccgatggtta tttgatggaa agtaatcatg atcttgagat gttacgcatg ggaccttatc
     8281 cgtggaattt aaaacagcgt attttaggag atagaggaca tctttccaac gaagacggtg
     8341 cacttgtcat gactgatgtg atcggtaatc ggaccaaacg aatctatctt ggccatctta
     8401 gcaaggaaaa caatatgaaa gaacttgctc atttaacaat ggaaaatgtt ttgaaagaaa
     8461 aagattttgg cgttggacat gattttgaaa tctatgatac agatccagat gaagcaactg
     8521 agctattttc gatttaaaca gttcaatcaa taaaaatata aaaagcagaa gataaaatca
     8581 atcagggttg gttttatctt ctatttttat ttttttagca tattaaaaaa ataaccgtat
     8641 tatcgactga ctacactgat gtgctgcttt tcatgtttct catccagtat ctcatctact
     8701 aatgcaatcg cgtaatctgc catgctgatc gtactgtcgc tagcagcgtt tctttgtaaa
     8761 taatcatcac ttaacaaata agtacctgta cgtttgccat gtggatcaaa taaagaagct
     8821 ggactgatat aagtccaatg tagattttta gattgtttca attttaacaa tccttcgtac
     8881 atattgaaag ctgttggata gaagggtgca tttttaggta cttggtcaat catacgagtc
     8941 gtcttttctg tatctaagaa taacgaagag gcacccccaa cgacaattaa gcgtgtatcg
     9001 gttccttcaa gcatctcaat caaatgggac aaagaagttt gatgcatttc ttcttttcct
     9061 ttgggagcgt tgaaagcatc aacgacaaca tcaaaagatg caagatcagt agttgtcaac
     9121 tcaaataaat ccttttgtat aaacggaaca tctgatggca atttttctgg atgacgcacg
     9181 attgccgtca cgtctaaatc tctggacaat gcttcgtcta aaataaatga acctgcatgg
     9241 ccagtagctc caatgagtgc aattttcata aaaaacctcc ttttttattc ctgtccattg
     9301 taagcagttg ctgaatattc gtcaaagaat ccgatttttg aaaagctcag tgcgcattcc
     9361 tttcatggtt cgtgatcatt gggtatagtt aaattaagaa aactgattga taaaaataga
     9421 agttgaagga gataagaaat gaaagagtgg ttagatcgtg cctttttacc aatgataaca
     9481 tctgttgtga atcttagacc cttaactgct ttgcgtaatg gaatgactta cacgatccca
     9541 ataattattg ttggctcgct gtttttctta tttgctcatt ttcctgtgga ttacatcgct
     9601 cagtggatag acaaaacagg attttctatg tattttacac aagtatacca ggctacattt
     9661 gatgtgatag ccttttggat cgtttttgcc attgcatacg cttatgcaaa agaagaagac
     9721 atcgatcgct taccagtcgg cataacagcc ttgtctgctt atctactgct tattcagcca
     9781 ctaaatactc agatagatac tgtctggaca ggtgctgaag ggatactagg agcagttaca
     9841 actgggctca tcatcggatg gggctatgca cgtttagtaa gacttgcgaa agggtggaag
     9901 caaagagaga agatacctga aaatgtttgg cagtctttta cttcattgat tccaatcatt
     9961 gttatagtca cttttacgct tatcggttca gcggtttttc aacatttgtt cagaatgact
    10021 gttgttcaag gagtatggac atatgtccag cttcccattc aatcgttcat agacacgttg
    10081 tttggtgtgc ttttattagg tttttttgtt ccgtttttat ggttatttgg tcttcatgga
    10141 tctgcgatgg tcaatgggtt ggttagtccc gtcttgcaag cgaattcatt agcaaatgca
    10201 gaaatcttag cctcaggaca agagttgacg gtggcaaatg gcggacatat cgttactcag
    10261 caatttctcg atcagttcat gacggttacg ggtgctggat tgacgttagg ggctgtattt
    10321 tttatgacgg tttttgcgaa atcgaagaaa tacagagaat taggaaatct tgctctttta
    10381 ccagcctttt tcaacatcaa tgaatcaatt attttttcca caccgatcgt gatgaacccg
    10441 atgatggctg ttccttttat tttcgcaccg gtcgtttcag gattgattac ctattccgct
    10501 ttatattttg gattcgttcc tttatttaca gcagtccaag tcccttggac aacaccgccg
    10561 atcctctcgg gtttcttagt aggcggagtg cctgcagcga ttttacaagg tatcgtgttg
    10621 tcaattagtt ttttcattta tttcccattt ttgaaaaaaa tagattctgc aaactgcaaa
    10681 aaggaaagac agacaaaaaa tgatggagca gcagaaaaga tgaacgacta aaaaacaaaa
    10741 atactcctgt cgctttagat tgggaaaatc accaatctag gatgacagga gtatttatag
    10801 tcatgtttat actagccctt tttggacagc taattgaaca agaacaacta atgtgttcat
    10861 tcctacgtgt gtgatcatcg atgtccagat ccgaccagtt gctttgtact ctaggctgaa
    10921 gaagaaacct aggaaaaagt agattagcag atgaccatca ttatgagcaa acgcaaacag
    10981 caaagagctg atcactgctc cgacccaaaa atttgaatat cgactgatga tccccagtat
    11041 actgcggcgg aatacgaact cttccatgat gggtccagca acagttgtcg ccaaaataaa
    11101 agcgggtgct tccatgatca tctggatgat attttgtgta ttctcggaag gggttgcttt
    11161 gccaaacaat acagattcta acatgacagc gatgctttgc agaatcaatg cgacaaagat
    11221 tccaaccaat ccataaataa caatctttgg agcagaagct ttttttaccg ctgattcgat
    11281 cgctaagggt tcacgttgct taaaataaaa aatagctagg acaacggcgc ctagtatata
    11341 catgactgca gttgctgtga tcaattcaga cgtagatttt acaagtccta tcgaagctag
    11401 gatcaagggt gagataaaaa ttagaccata gcaaaaaatg ctgaggaagc tatatttttt
    11461 taaagacatg taattcctcc ttttaaggga taacaagcat gcaaggcaag cttatatcga
    11521 ttgatttcaa ttatatcata atttcaataa gcagctgtca gaataaaatt tcttttcttt
    11581 gatttaaaag cttgcaaaaa agagggagaa tgagtattat taaaaatgta ggttagcact
    11641 cgaaggttaa gagtgctaac agattaaaaa aagagttatc ggagggattt atcgtgttaa
    11701 aaccattagg tgatcgcgtc attatcgaag tcgcaaaaga agaagaaaaa actgtcggag
    11761 gaatcgtttt agcttctgct gctaaagaga aaccccaaac tgggacagtt gttgcagtcg
    11821 gtgaaggccg tttgttagaa aacggtgaaa aagttcctgc cgctgtaaaa gcaggcgacc
    11881 aagttatgtt cgaaaaatac gccggtacag aagtaaaata cgaaggaaaa gaatatctga
    11941 tcgtcgcagg aaaagatatc atggcgattg ttgagtaaat ttggaagata aaaaaataat
    12001 caaaaataat caatgaggtg aatcaatcat ggcaaaagaa ttgaaatttg cagaagacgc
    12061 acgcgcagct atgttacgcg gggtagataa attagcagat acagtgaaag taacattagg
    12121 acctaaaggc cgaaatgtcg tactagaaaa atcttacggc tctccattga tcacaaatga
    12181 tggtgtgacg atcgcaaaag aaatcgaatt agaagatcat tttgaaaaca tgggtgcaaa
    12241 acttgtatca gaagtagcat caaaaacaaa tgatattgca ggagacggta caacgacagc
    12301 aactgttctg acacaagcaa tcgttcgcga aggtttaaaa aacgtaactg caggtgctaa
    12361 tcctctaggc attcgtcgcg ggatcgaatt ggcaacaaaa gcagcagtcg aagaattgca
    12421 taatatctca actgttgttg attcaaaaga agccattgca caagtagcag ctgtttcttc
    12481 gggttctgat aaagttggac atttgattgc tgatgcaatg gaaaaagttg gaaacgacgg
    12541 tgtcatcaca atcgaagaat caaaagggat cgaaacagaa ctagatgttg tagaaggtat
    12601 gcagtttgat cgtggttact tatcacaata tatggtaaca gataacgaca aaatggaagc
    12661 tgttctagaa aacccatata tcttgatcac agacaaaaaa atctctaaca tccaagatat
    12721 tctgccattg ttagaacaaa tcttgcaaca atcacgtcca ttgttgatta ttgctgacga
    12781 tgtagatggt gaagcattgc caactcttgt attgaacaaa atccgtggaa cattcaacgt
    12841 agtagctgta aaagctcctg gtttcggcga tcgtcgtaaa gcaatgcttg aagatatcgc
    12901 tattttgaca ggtggtacag taattactga cgatctaggt ttagaattga aagatgcaac
    12961 aatcgaaaac ttaggaaatg cttctaaagt tgttgtagat aaagacaata caacgatcgt
    13021 agaaggttct ggagaaaaag aagcaatcga agctcgtgtg caattgatca aaaaccaaat
    13081 tgctgaaaca acttctgatt ttgaccgcga aaaattacaa gaacgcttag ctaaattagc
    13141 aggtggtgta gctgttgtta aagttggtgc agctactgaa acagaattga aagaattaaa
    13201 attacgcatt gaagatgcat tgaatgctac acgtgccgca gtagaagaag gaatggtctc
    13261 aggtggtggt acagctctag tgaacgttat cagcaaagtt tctgctgtag aagcagaagg
    13321 agacgtggca acaggtatca agatcgttgt tcgtgcttta gaagaaccaa ttcgtcaaat
    13381 cgctgaaaac gctggttatg aaggatcagt gattgttgat aaattgaaga acgtggaact
    13441 aggcactggt ttcaatgctg ctactggaga atgggtaaac atggttgaag caggtatcgt
    13501 agacccaact aaagtaacac gttctgcctt gcaaaatgcc gcttccgttt ctgccttgtt
    13561 attaacgaca gaagcagtcg ttgcagataa accagaacca gcagcgccag cagctcctgc
    13621 aatggatcca tcaatgatgg gcggtatgat gtagtaatta agaaagaacc ctattttaac
    13681 agggttcttt cttctttttt tgttttattt taagcaaaag gggcaaagaa ggggcaaaaa
    13741 tatcaaaggc tatttagttt atcaattact tgttgtttgg ctctttttgt tacatggtta
    13801 taaatagaca gagttgtgtt tgcatcagaa tgccctacac gctccataat ggctttaaga
    13861 ggtacgccta attcagataa tagtgaaacg tggctatgtc tgaatatatg tgaggataga
    13921 cttttttcta attccaactc ttcctctact ttatggagta tagcgttaaa tgagtgcagc
    13981 gcaagcggag tgccacttgt agatataaat atatattgat cgggatctgt gggtctacct
    14041 gcaagaatat tgtcagctat cacactttca atcaattctt ttgcacgatt gggtaattgc
    14101 acttcacgtt gcgaataagt attttttgga gttgttttta tagcattatc cattttcaca
    14161 gatgtgtaat ctaaggtccc attaatggaa attttcccat cctcatagtc cttcatttgc
    14221 aaagctagca attccccata tctcaaacca gttaaatata gaaactcagc tattatgccg
    14281 tgtagttttc tgcgaggatt ggagtatagc tgtttcagta tttgatcaat ttcttctttc
    14341 tccagatatt ttttattcat agaaagcctt cttttttctt cttccacttt tttaggatgg
    14401 attttaactg ctagcgcagg gtttctttga atgtattttc tatcgattgc atagtttagc
    14461 ataacagata gagttgtttt tgtttgtttt gtgtagttca gtgagaggtc accaaacgta
    14521 tacatatctt caattatctt attaatgagt gtctcatcaa tgtttctaac gatcgtatca
    14581 tcgcttatgt gcttagaaac atgtttcatc atcattggaa ccttcaaata gctagtacgt
    14641 ttaacatgct gcttataata ttcataccat tctttataca gctcaccaaa agtgatatct
    14701 gatttattgt aatcttcgag tgcttcttta atttttttat ctagaatttt ctgagctttt
    14761 ttccacgctt gtggtgaatt acttgtaagt gttgtagatt tttttcgtgt tttttctgta
    14821 taaggatcta catatctttc aataaacttg aattggccat ctttggtctg ttcaacccac
    14881 acttttaaca tctcctatca tttgctataa taggcataac aaatagacct atataggttt
    14941 gttttctaaa agcacgctct tactctggac ggtggggcgt gttttttatt tattatacag
    15001 ttgctttaaa aatagctgct ttttcaaatg atactaaagg agagaagtgg atttctattt
    15061 ctcccttttg gtttaacgtg aaatgagcag ttacatccat ttgttttcct ggagcaacag
    15121 atcccattgt attttcgttg gcatatgtct ctgatttttt gtcatctggt ccatatactt
    15181 ccacatctgt acctacaggg atatctgaat cgccatcatt ttttacggta taagtaattt
    15241 ttactacttg tgcaggttgg ttttcttcaa attgatttct ttcatcagtt agttctacac
    15301 tatttagtgt atattcagca tcaccaactt ttacagtgtc accaatctta tagaaggtat
    15361 cgcttttttc ttctttagaa gatgaagcag aggttgaaga tttagttact ttttcgccac
    15421 cattatcact tgctttatta ctatctgatc caccatttaa agcagaacca ataattataa
    15481 ttaaaattac tgctaatacc caaaaccata cttttttgta aaaaggttta ctaactttat
    15541 acatttttcc gtcttgaccc ataacttttt ttgccattta aatattcctc acttcttgtt
    15601 ataatatatt tgcgatctca gaaatgaggt atgagtccgt gtgccagcac gggctttttt
    15661 aatgttttgc tgaaatcggt ttttttaata attcttgatg tattcataca tattgccttg
    15721 cgtaagaata ttttttctta aaatagcatt ggcagacagc ataacaagtg tatctgagct
    15781 tattctgaat agaataatac tccataaatt tttcgagatt aaactgagat tcatcagtca
    15841 gttcgttctc aatatagata tttaataaaa ttagaatagc tattttatct gcttctgttt
    15901 caaattttga gtgaaaagtt gtagaagtat cgtacaaaac tgaatattca aaatgagaag
    15961 cggtgaaatg cgcaagttca tgagataaat gaaaggcttc tgcagtttcg ccatatagat
    16021 tttcattcaa aaaaatgatt ctaggcttcg gataatagaa gccaggctct ttcatttcca
    16081 tgtaaactac tttcaaattg tattcgctca acatttcttt caatttcaaa tacatacaac
    16141 ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg ctttacgcat
    16201 tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtttt ctgataaatc
    16261 cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag agactccaaa
    16321 gtaatcagct aaccgagcta actcaataga attaggagtt tgtcttttcc aacttcctaa
    16381 atatccattc gaatacccga aattcatttc gagctgtcta atggataatc ctttttcttt
    16441 tgctaactcc tttattattt cgtaagtatt cattgataaa tcaacctttc tgaatgctta
    16501 caaaaaaagt ttagataaat aatgtaaaaa tagttgactt atatacatta ttaatctata
    16561 ctatgttttg taaacaagtt taacaactaa aaagacaaca aaaaatacta ttgattaata
    16621 aatgccaacc gccaagaaag ctttttaaat caaagtttat atgtcttatt taactatgca
    16681 tttagtatag attattaatc taataaagtc aacgcagttt tttaaaaagt tgttaaattt
    16741 gtttacgaat ataaaagaaa ggagagaaga atatggaaaa aacagtctcg gcaaaaatcg
    16801 aagatttgaa attagacata ctgaaacaag caaaagtggc gatggaacac gcggtagata
    16861 aagaagactc tgccatggtt gcagccatag cagagatttt agctcacgtt tagtcatcgt
    16921 ttttacggtc ttctggaagc tgaccataga taacagcata atgaatatct aaataagctt
    16981 gaacaatttc ttttggcgaa attgattcgc ctttagtaga tacttgtgat tcgtgattta
    17041 gccatgcaac aactaaatcc gcagcgattt caaacggcgt tacactttgt gtcagttgcc
    17101 ataaagaatt tcacaatatg tatggtcgtg gtaataatac caaagagcaa tttgaggaat
    17161 ttatggaaat taaaaaatac aaaaaatcat ttattaaagg aggctaaaca atgaacaagg
    17221 aaatgagcca aacaatcaag gtacaaaaaa tgatagatga tctaacacat ggaattgata
    17281 gccaagccga taaaatcatc aaggaattgc aaggacaaaa agttaaggat gcaaaaatgc
    17341 ttctcaagac tatcaacttt gaaatgaatc caacaaaaag aaagctcgcc gatgtgttgg
    17401 aagaaaagtt ggcttccgct atcaacgagc aagaattact atttgaaacc gatactttta
    17461 acagctgatg ttttatagat gacagtttca tccgaatcta tatcatcgcc gagagaaaag
    17521 aatgttgctt ttgacaaaaa gccgataaga cctacaagcg gtcttgatgt tgcaagagta
    17581 gataccccaa tatcaccttg caaggtattc acgtaaattt gagaagaata gaacattcca
    17641 tctttttctt cgatatttga cgcatcatcg tatccaacga ccaaagtatc ggcaaatatt
    17701 ttgatttttt ctccatcaag catagtaatt acaaattctg gtttcataaa ttttcacctc
    17761 cttaacaatt atttcagcct gtcacactga taaggaaatt ataccaaaga aaggaatgag
    17821 aaatatgaat acaccacaaa tttttaattt cgagcaaaac gaagttcgaa ctattttagt
    17881 aaatgatgaa ccatattttg taggaaaaga tgtagcagac gttctaggat attcgaatcc
    17941 gcaaaaagct attcgagatc atgtggactt ggaggataag acgcagaacg attcgttcac
    18001 cgtcaatgga acagcagttg ttttgattaa cgagtcaggc ctttacagtt taatcttaaa
    18061 atcaaaactt ccctctgcca aaaaatttaa acgttgggta acgagtgaag tccttccaac
    18121 aattagaaaa acaggtagct attcaaacgt acctcaaagt tttgcacaag cattgcgttt
    18181 agcagcggat ttagaagaaa agaaccaatt actcgaacaa caaattgccg agtacgaacc
    18241 aaagattagc tacttagata cgattctttc atcgacagat acggtagcta cttctcaaat
    18301 tgcagctgat tacggaatgt cggcaattgc tctaaacaaa ttgctaaacg agttaggtgt
    18361 tcagcataaa gttagcggac aatggatact ttaccgaaaa catatgaacc aaggatacac
    18421 aaaatcgcac acaagtgaga taccgaaagc cgatggcggc actaaagttg taatgaatac
    18481 caaatggaca cagaaagggc gagtgtttat ttacaactta ttaatcgcag agggctatta
    18541 ccctcaaatg gatttagagg aaattggtta gaaaggagtt ttagtatgac tgacattgca
    18601 gaaatcactc aacgagatag agaaaaaatc aaagaatatg tcgaaagttc aaagttctta
    18661 acttacacca tgcttgctga aagatttgga attagcaaaa gctacttatc tttaatttta
    18721 aacggtaaaa agacttctgc agaagcaaac agaattatag attcgattat cactatgtac
    18781 gaattgtaga gggaggtaaa gtcgttggaa gataaaatca ttcttacaaa atcagagctt
    18841 caagaaatgt taaacatcga gtatggaacg ataattttta ggagggataa taagtgaaaa
    18901 aaccaacact ttcagagctg atagaagctg ctgagaaggc agtaaaacca gacgactggt
    18961 accgacaaag tttaatcttg gagaagttcc acggcatgtc aaaaactact ttagttgaat
    19021 actgcaagga aatggaaaca attcctgaat ttgcagaagg aatacttcgt ccaggtcact
    19081 caaccacatt tattcattac catactttta tttggttttt aaagtggaaa gacgcaaaca
    19141 aatatcgtgt aaaaagttta tcgccttcag atgttttgaa ggaggcaagt taatgggcaa
    19201 attcaacaga gcactagtat tcagcgcacc gctaatcatc tacgctttag gcctctgggg
    19261 aagcagacaa gcgttgatag ggacaatcgt ttacatggtt tggattttta tggggcttga
    19321 tgaagctgag cacagagcga aaaaagccag tcgggaggga ctgactaaaa tatgattttt
    19381 ttatttttgt tcctttgggc tgctattgcg tacttcatcc agctccttgt tgtcaaagtt
    19441 gctcttattt cttttaggaa gaacaaaaga aaatagcgct ataaatgaaa gataagtagc
    19501 cgctacatta aactgttctt ttgaaaaagt tacagtcatc ggaatgataa ttgatattga
    19561 ggaagtaatc ttaattaaaa atatagcaat attttcaaca tcgcgttggt ttttatttaa
    19621 cattgtccaa catgttttag caacaataaa aataattaca catgatatta gcacacagac
    19681 tgaaaaaagt tttagaaatg cgatgtagtc agtgttattg aactccttca aaatactatt
    19741 tttattcaat gtaataaaag gaattttttt aattgaagac aggaaagata aggtaataaa
    19801 cagaattata tatatggtta aagtcgcagt aagaatgtaa ttgagctttt tcctcataga
    19861 atcacctcac tttctaacga aaattatacc aaaaaggaga gaagaaataa tgcaagaatt
    19921 agtaattttg aaaaataaag aagctgtaac tacgagctta caagtggcag aaagtttcaa
    19981 aaagaaacat aagcatgtac tagaagcaat tgaatcaata aaaagatcgg tcgaaaattc
    20041 ggccaatgtt gaagatgggt ccaattttgg acagatgttt gtggaaggga acgagccaga
    20101 ctcatacgga agaagtcgga gagtttattt catgaataga gacggatttt ccttgctagc
    20161 tatgggattc actggaagta aagcaataaa tttcaaacta aaatttattg aagctttcaa
    20221 cgaaatggaa gatgttattc ggaagaatac tgttcctcaa acaattgaag acatgatgat
    20281 ctatcaatta gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat
    20341 gcgaattagc ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatgga
    20401 agttctaggg ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa
    20461 attttggtct gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa
    20521 gagattcgat gatgctgttt catttattga aatgtggtta ccagaaactg cgatccgcat
    20581 ggaaatcgat caactgaaca gacaacaaag acttttcggt gatgaaaatg aatagagctg
    20641 aagcgctaag aatagggacg gtaattgcta atcgctggtg gagacacaat aaaccaaaca
    20701 tcctaagcca acaacatatc gaaaagcaaa aagcatggca acaaataaaa aagtgactca
    20761 gccggcaagc atagagtcac aaaacatatc taaggagaat tttagcatat gaataaagaa
    20821 ctttccactt tagatcaata tttgactgat tctgaatggg gcaagtcgaa tatcaaggaa
    20881 acaaataatc gaaaaatcag acgaaatctt ttgacgaacg aagaactatc atgtgatcaa
    20941 gatgacttgg gaaattttgt gagtatttgg gatcatgttt accttatcca tctatcaaaa
    21001 cattcaaata aacctgaata catctatgtc atcgaagatg gcttgattga tgcgctagaa
    21061 gagtacaaca gagataactt gattgatatc tcttattacg gatcaggtaa gaaatacatt
    21121 gctgaaatgg aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact
    21181 ttttagtcgt aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg
    21241 ggctcacgcg tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga
    21301 gtataaacac tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa
    21361 accttttctt atggatgaaa cagggactta tgtatctgtc tcagtaacgg ttaaaggaca
    21421 tacggaaaca ga
//
LOCUS       DACDTO010000045        20654 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDTO010000045 DACDTO010000000
VERSION     DACDTO010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20654
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.045"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IWK70_001671"
     CDS             complement(<1..626)
                     /locus_tag="IWK70_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9614203.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPR"
     gene            899..3370
                     /locus_tag="IWK70_001672"
     CDS             899..3370
                     /locus_tag="IWK70_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9614204.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3473..5209)
                     /locus_tag="IWK70_001673"
     CDS             complement(3473..5209)
                     /locus_tag="IWK70_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9614205.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5206..5910)
                     /locus_tag="IWK70_001674"
     CDS             complement(5206..5910)
                     /locus_tag="IWK70_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614206.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            6041..6544
                     /locus_tag="IWK70_001675"
     CDS             6041..6544
                     /locus_tag="IWK70_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9614207.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6504..6842
                     /locus_tag="IWK70_001676"
     CDS             6504..6842
                     /locus_tag="IWK70_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9614208.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6863..8281
                     /gene="ffh"
                     /locus_tag="IWK70_001677"
     CDS             6863..8281
                     /gene="ffh"
                     /locus_tag="IWK70_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9614209.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8393..8971
                     /locus_tag="IWK70_001678"
     CDS             8393..8971
                     /locus_tag="IWK70_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9614210.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8975..9496
                     /locus_tag="IWK70_001679"
     CDS             8975..9496
                     /locus_tag="IWK70_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9614211.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9575..10129)
                     /locus_tag="IWK70_001680"
     CDS             complement(9575..10129)
                     /locus_tag="IWK70_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9614212.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10382..10657
                     /gene="rpsP"
                     /locus_tag="IWK70_001681"
     CDS             10382..10657
                     /gene="rpsP"
                     /locus_tag="IWK70_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9614213.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10669..10920
                     /locus_tag="IWK70_001682"
     CDS             10669..10920
                     /locus_tag="IWK70_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9614214.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            11045..11836
                     /locus_tag="IWK70_001683"
     CDS             11045..11836
                     /locus_tag="IWK70_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614215.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            12006..12527
                     /gene="rimM"
                     /locus_tag="IWK70_001684"
     CDS             12006..12527
                     /gene="rimM"
                     /locus_tag="IWK70_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9614216.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12517..13278
                     /gene="trmD"
                     /locus_tag="IWK70_001685"
     CDS             12517..13278
                     /gene="trmD"
                     /locus_tag="IWK70_001685"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9614217.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13414..13761
                     /gene="rplS"
                     /locus_tag="IWK70_001686"
     CDS             13414..13761
                     /gene="rplS"
                     /locus_tag="IWK70_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9614218.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13893..15029)
                     /locus_tag="IWK70_001687"
     CDS             complement(13893..15029)
                     /locus_tag="IWK70_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9614219.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15145..16059)
                     /locus_tag="IWK70_001688"
     CDS             complement(15145..16059)
                     /locus_tag="IWK70_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9614220.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(16150..16578)
                     /locus_tag="IWK70_001689"
     CDS             complement(16150..16578)
                     /locus_tag="IWK70_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9614221.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(16583..16957)
                     /locus_tag="IWK70_001690"
     CDS             complement(16583..16957)
                     /locus_tag="IWK70_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9614222.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17243..17428
                     /locus_tag="IWK70_001691"
     CDS             17243..17428
                     /locus_tag="IWK70_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9614223.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17440..17688
                     /locus_tag="IWK70_001692"
     CDS             17440..17688
                     /locus_tag="IWK70_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614224.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            17719..17898
                     /locus_tag="IWK70_001693"
     CDS             17719..17898
                     /locus_tag="IWK70_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614225.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(17941..18411)
                     /locus_tag="IWK70_001694"
     CDS             complement(17941..18411)
                     /locus_tag="IWK70_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614226.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            18498..19196
                     /locus_tag="IWK70_001695"
     CDS             18498..19196
                     /locus_tag="IWK70_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9614227.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            19374..19715
                     /locus_tag="IWK70_001696"
     CDS             19374..19715
                     /locus_tag="IWK70_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614228.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            19708..20379
                     /locus_tag="IWK70_001697"
     CDS             19708..20379
                     /locus_tag="IWK70_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9614229.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            20385..>20654
                     /locus_tag="IWK70_001698"
     CDS             20385..>20654
                     /locus_tag="IWK70_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614230.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
BASE COUNT         6792 a         3621 c         4070 g         6171 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gattggttgt cccatcaaca caaataaaac tcatggaacc agaaacagaa
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      361 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      421 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      481 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      781 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      841 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      901 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      961 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
     1021 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
     1081 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1141 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1201 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1261 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1321 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1381 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1441 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1501 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1561 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1621 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1681 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1741 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1801 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1861 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1921 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1981 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     2041 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     2101 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2161 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2221 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2281 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2341 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2401 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2461 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2521 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2581 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2641 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2701 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2761 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2821 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2881 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2941 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     3001 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     3061 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3121 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3181 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3241 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3301 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3361 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3421 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3481 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3541 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3601 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3661 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3721 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3781 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3841 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3901 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3961 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     4021 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     4081 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4141 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4201 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4261 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4321 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4381 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4441 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4501 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4561 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4621 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4681 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4741 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4801 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4861 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4921 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4981 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     5041 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     5101 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5161 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5221 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5281 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5341 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5401 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5461 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5521 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5581 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5641 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5701 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5761 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5821 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5881 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5941 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     6001 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     6061 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6121 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6181 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6241 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6301 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6361 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6421 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6481 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6541 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6601 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6661 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6721 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6781 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6841 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6901 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6961 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     7021 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     7081 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7141 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7201 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7261 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7321 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7381 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7441 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7501 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7561 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7621 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7681 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7741 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7801 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7861 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7921 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7981 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     8041 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     8101 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8161 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8221 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8281 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8341 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8401 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8461 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8521 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8581 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8641 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8701 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8761 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8821 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8881 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8941 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     9001 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     9061 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9121 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9181 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9241 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9301 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9361 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9421 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9481 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9541 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9601 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9661 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9721 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9781 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9841 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9901 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9961 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
    10021 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
    10081 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10141 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10201 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10261 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10321 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10381 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10441 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10501 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10561 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10621 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10681 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10741 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10801 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10861 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10921 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10981 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    11041 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    11101 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11161 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11221 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11281 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11341 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11401 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11461 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11521 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11581 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11641 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11701 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11761 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11821 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11881 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11941 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    12001 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    12061 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12121 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12181 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12241 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12301 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12361 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12421 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12481 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12541 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12601 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12661 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12721 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12781 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12841 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12901 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12961 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    13021 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    13081 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13141 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13201 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13261 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13321 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13381 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13441 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13501 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13561 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13621 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13681 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13741 atcaaagaaa tccgtcgtta atcatcgtta tcagactgag aaagaaccct tggtattcaa
    13801 gggttctttt tttgttctct gtttttttat attaacgatt tctatttggg acatttttga
    13861 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13921 ttttctttta ctccctttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13981 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    14041 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    14101 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14161 agaaattgac cattcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14221 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14281 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14341 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggcctcat tccagtcaaa
    14401 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atttactagt
    14461 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14521 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14581 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14641 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14701 atgtaagtct caggtatctt tttctttata gtgtttaaga tattattcgt aggataatat
    14761 gtagactcct taacttgtct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14821 gttagatcag gcttctgaag tgttttttcg tttaatttta tatcaatttt tttatttaac
    14881 tctagcaatg ccactttctg agtttctcga gatttatttt tataagtaat gctcactttt
    14941 tttctttttc tagtttttgg atcaatatat cgctcattat atttatatac tttttttccg
    15001 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    15061 gaatggataa gtaagccaat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15121 gagggagggg gcgtgttttt ttgtttagta gctacctttc catgaagtat ctgcgaagaa
    15181 cacttcaaat gttgttccat catttttcac ttcaaaatat gctgttcctt gtgcggattt
    15241 acccggttgg atggtttccg aaaagtaatc tctactagct aaatcagatt taatatctga
    15301 tgaatcataa aattctaaat aacttgaact tgcatcaaaa ggtgtagtcc ctgtgttttc
    15361 tacaagaaaa tctacctttg cgtaaaatag gccttctggt ttatagaaat catcaccgta
    15421 atcttttgat acagactgga cagttacgtt aatagagtta ccattttcag aagtaaaagg
    15481 aactgcttct cctatactat gtgcaactga tgccgtctgg ggttctgaag acgaagttgt
    15541 agttgtagtt gtagtagagc ttgaatcttt ggattttcca tctttatcta agaaagttcg
    15601 ttgattataa tatacaacct gttcagtttt tggactaacc caaattaaca ttgtttcatc
    15661 atcagaatca gttgaataag tatattggag catctctaat ttttttatat cttttgcact
    15721 atttccatgt tcaattaatt cagagtgctc tttataataa tctccttgtg catcctttga
    15781 aatagataca attaattcat cccgtgtgat agcattgtct aattctttgt aaacaaaatc
    15841 atttgtccat ttacgtaacg gtgaaccaag tgtctgataa acttgagtta aatcatcacc
    15901 aacttttatt ttatgaacgt ctgatgaagt tagttttttt gtttcatatt cagatgttga
    15961 tgaaacagta ttttctttag tgctagcaga attaggacta gtggagcatc cgcttaatac
    16021 caagcctaaa aaaagcaacc caaaaacaat ttttttcatt tttaattcct cacttctatg
    16081 atatgatttt tttgtaggat cttagaaata aggtttttag tccgtgttgc agcacggact
    16141 ttttttacat tatataagaa agcgataagc gctttctgga agtccgtaaa gattctttaa
    16201 ttcctcaatt cgtttaggat attgatcatt gtcttctttg tatagagaaa caatgagatt
    16261 agcagcaaag caattagctt cgctttcaga tttgcttcta gatgttcttg ttgatacata
    16321 gtaactggat aagccacgat gaaaaatagc gtgacctaat tcgtgagcgc aaatgtagaa
    16381 tctttcctca gagtctctca gttcatcatt taagaagatt attgcacgac ctctaatttc
    16441 ttgaaactgt cctttgggat tctcgataaa aggaacgtat tgaattttaa tgcccatctt
    16501 ttcacaaata taaaaaggat tagcggactg gtatttccgc ttcaactcct cgactaaatt
    16561 aatcgtatcc atctccataa gctcacatct ttttgccttt ttctttgtct tctttcacaa
    16621 tatcccagaa ggttccaata aggatatctt ttacgcgttg gacctgttca ggcgtcagtg
    16681 tttctccgcc ataagacata ttgacatttg aatctagtaa tttatcaagt tcaactactt
    16741 catcttttgt tgcccagtca ggaacttggt ttctaccgag taaataatca gtagttacat
    16801 taaaataatc tgctacaagt tgtagtcttt tagtactagg agttctattt ttccattgat
    16861 aaatcgtgtt tttaggtata tttaattctt cttccaattg agttatactt atgcctcttt
    16921 tatgagctaa ttcctttatt ctatctagta aattcatttt cgcacctcac aagctacgaa
    16981 aacactaata aaaaaattag ttaaaggtgt tgacaactaa taaaagtgtt agtatactgt
    17041 tttcgtaagc taaaatatta gctaaaaaga cttgataact aataaacact tcacggtcgg
    17101 caaactctga aatgtaaatt actaggcatt tctgtgtctt atttagctat gtctatatac
    17161 taataaaaat attagttatt gtcaacgaat attagctaat tttttagctt acagattatt
    17221 tttttagaaa ggagctattt ttatgtctga gaatttagac ttaaaaattc gagcggagat
    17281 gagaaaaaga agaatgactt tcaaagaact agctgcgctt atcggtattt caggagctta
    17341 tttatcagat attctaaacg gcaatcgtga tggaaagaaa gcacaacagc atatcgaaac
    17401 agtgaaaaaa atattggaca tccgataggg gggtaggcga tgacaaaact aaaaaaacaa
    17461 gattttgtaa aaaaatacaa ttattctcca tctacttatc aacgtcgaat gtcggaacta
    17521 aaaaatacag caattttctc agcggcgtat gaacgggtca caggacaaga agtttggatc
    17581 aatacagaat tatacgataa atttttgtct ttcaaatcct ataacaggtt acgcacaaga
    17641 aaggtaacac ctaaagaatt tatcgagaag catttagttg atttataaaa aacagaacat
    17701 tttgagaggt gaaggttaat gggtaaattc aacagagcat tggtattcag cgcaccgcta
    17761 atcatctacg ctttaggact ttggggaagc aggcaagcgt tgataggaac gattgtttac
    17821 atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca
    17881 accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac
    17941 tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat
    18001 tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt
    18061 attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg
    18121 aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc
    18181 aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc
    18241 taaattaact aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat
    18301 catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt
    18361 tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa
    18421 aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa
    18481 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta
    18541 cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg
    18601 atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag
    18661 attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg
    18721 ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca
    18781 acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga
    18841 tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta
    18901 tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg
    18961 gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa
    19021 aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta
    19081 agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta
    19141 tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct
    19201 gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc
    19261 atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc
    19321 cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag catatggaaa
    19381 aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg
    19441 aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg
    19501 atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat
    19561 cgaagcattc gaataaacct gaatatattt acgtcatcga agatggcttg attgatgcgc
    19621 tagaagagta cgacagagat aacttgattg atatctctta ttacggacca ggtaagaaat
    19681 acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac gaactttgaa
    19741 aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta
    19801 tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt
    19861 aatgagtaca aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa
    19921 tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt aacggttaaa
    19981 ggacacacgg aaaccgaatt atttcctgtt ttagattatc gaaaccaacc agttgttaaa
    20041 ccaaatgcaa tgcaaatcaa taactcattg aagcgatgct ttgtgaaagc attggctcta
    20101 cacggactgg gattatatgt atttcaaggg gaagatattc caacaccacc tagaatcgat
    20161 acaaagaaat taaacatgct agagacgatt ctagaagctt tcaatgagca gatgggtaaa
    20221 gatatgacca aaaccttaat cgaatatgtt aatgagcaga cagataaatt agggctctta
    20281 gctgataacg ttgaaactat tgaacagtta agctatgagc aatgtgcctt gatggagcga
    20341 gcaatagcag ctaagagaaa agaattagat aagaagtgat atgagtgttt aaaccattaa
    20401 tcgattcata ttcagcggtt ctgaaaaagt tcaaaggaaa agacataagc gcaaccatca
    20461 atgaggaagt gaacattgat cgactaaaga cgatgtatga cggctacgat ggtgatcgag
    20521 tcattgaaat tcgttttatt gatcctagac gtttcaccgt acagcaacga aacttcatct
    20581 atgcgctgat aggcgatatt tttatcgata caggcatgcc aacggacttc tggaaggaat
    20641 tcttctactt ccgt
//
LOCUS       DACDTO010000046        20485 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDTO010000046 DACDTO010000000
VERSION     DACDTO010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.046"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="IWK70_001699"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="IWK70_001699"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9614231.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="IWK70_001700"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="IWK70_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9614232.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="IWK70_001701"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="IWK70_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9614233.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="IWK70_001702"
     CDS             2857..3732
                     /locus_tag="IWK70_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9614234.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWK70_001703"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="IWK70_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9614235.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="IWK70_001704"
     CDS             4629..5063
                     /locus_tag="IWK70_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9614236.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="IWK70_001705"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="IWK70_001705"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9614237.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="IWK70_001706"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="IWK70_001706"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9614238.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="IWK70_001707"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="IWK70_001707"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9614239.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="IWK70_001708"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="IWK70_001708"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9614240.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="IWK70_001709"
     CDS             13334..14260
                     /locus_tag="IWK70_001709"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9614241.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="IWK70_001710"
     CDS             14442..15335
                     /locus_tag="IWK70_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9614242.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="IWK70_001711"
     CDS             15469..15594
                     /locus_tag="IWK70_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9614243.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="IWK70_001712"
     CDS             complement(15728..16360)
                     /locus_tag="IWK70_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614244.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="IWK70_001713"
     CDS             complement(16508..17050)
                     /locus_tag="IWK70_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9614245.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="IWK70_001714"
     CDS             17185..17574
                     /locus_tag="IWK70_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614246.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="IWK70_001715"
     CDS             17578..18342
                     /locus_tag="IWK70_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9614247.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="IWK70_001716"
     CDS             18471..19418
                     /locus_tag="IWK70_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9614248.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="IWK70_001717"
     CDS             19548..20288
                     /locus_tag="IWK70_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614249.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DACDTO010000047        20368 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDTO010000047 DACDTO010000000
VERSION     DACDTO010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.047"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            297..1406
                     /locus_tag="IWK70_001718"
     CDS             297..1406
                     /locus_tag="IWK70_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9614250.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            1489..2385
                     /locus_tag="IWK70_001719"
                     /pseudo
     CDS             1489..2385
                     /locus_tag="IWK70_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            2401..3225
                     /locus_tag="IWK70_001720"
     CDS             2401..3225
                     /locus_tag="IWK70_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9614251.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            3257..4567
                     /locus_tag="IWK70_001721"
     CDS             3257..4567
                     /locus_tag="IWK70_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9614252.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            4715..5974
                     /locus_tag="IWK70_001722"
     CDS             4715..5974
                     /locus_tag="IWK70_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9614253.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            5984..6856
                     /locus_tag="IWK70_001723"
     CDS             5984..6856
                     /locus_tag="IWK70_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9614254.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            6868..7698
                     /locus_tag="IWK70_001724"
     CDS             6868..7698
                     /locus_tag="IWK70_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9614255.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            7738..9921
                     /locus_tag="IWK70_001725"
     CDS             7738..9921
                     /locus_tag="IWK70_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9614256.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            9982..11202
                     /locus_tag="IWK70_001726"
     CDS             9982..11202
                     /locus_tag="IWK70_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9614257.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            11199..12659
                     /gene="gtfA"
                     /locus_tag="IWK70_001727"
     CDS             11199..12659
                     /gene="gtfA"
                     /locus_tag="IWK70_001727"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9614258.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            12736..13842
                     /locus_tag="IWK70_001728"
                     /pseudo
     CDS             12736..13842
                     /locus_tag="IWK70_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            14164..14373
                     /locus_tag="IWK70_001729"
     CDS             14164..14373
                     /locus_tag="IWK70_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9614259.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            14366..14515
                     /locus_tag="IWK70_001730"
     CDS             14366..14515
                     /locus_tag="IWK70_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9614260.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            14550..15334
                     /locus_tag="IWK70_001731"
                     /pseudo
     CDS             14550..15334
                     /locus_tag="IWK70_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="IWK70_001732"
     CDS             complement(15618..16703)
                     /gene="xerS"
                     /locus_tag="IWK70_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9614261.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            17037..17270
                     /locus_tag="IWK70_001733"
     CDS             17037..17270
                     /locus_tag="IWK70_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9614262.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            17267..17674
                     /locus_tag="IWK70_001734"
     CDS             17267..17674
                     /locus_tag="IWK70_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9614263.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(18811..19329)
                     /locus_tag="IWK70_001735"
     CDS             complement(18811..19329)
                     /locus_tag="IWK70_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9614264.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         6903 a         3140 c         3789 g         6536 t
ORIGIN      
        1 taaaaaagta aacatccaaa aaagggtgtt tacttttttg tttactaata tttttagtaa
       61 aaaatctcga caaaacatta agtaaacagt tatggtgttg ttaacaaaaa gaaaacgcat
      121 tcaaaacagg aggaattaag atgaaacatt tcaaaaaaat agctttaaca gggatagctg
      181 ttttaactgg gacattactc gcagcatgtg gtgggggaga tagtaattct aaagacgggc
      241 aagtaacttt aagctatgcg agttgagata ctgaccaagc tccaggacta agaaaaatgt
      301 tagatgaatt tgaaaaacaa aatcctgata tcaaagtaga aatggagaca acgccttggg
      361 atcaatattg ggttaaatta gaagctgcaa caacgggagg taatatgcca gatgtagtaa
      421 ccatgcactc tagcgaatcc tataaatata tgtcacagaa tgctttgatg ggacttgatg
      481 atgttatcaa agaaaatgat attgacatgg gaaactttac tgaaggaatt gctgatttct
      541 atacgcttga agacgaacta tacgcaatcc ctaaagatgc ttctgtagtg gggctatggt
      601 acaacaaaga attatttgat aatgcaggtg tcgattatcc agatgaaaca tggacatggg
      661 aaacactcag acaagctgca attgatttaa cagatgaatc aaaaggcatc tatggttttg
      721 catcacatat tggaacagag actggctact ggccatttat ttatcaaaat gggggagaag
      781 tatttgccga tgacaataca aaatcaggtt tgaatacccc agaagcacaa gaagctttag
      841 ctttttatcg agatattgca cttgttgatc atgcttcacc aacaattaca gaattacaag
      901 atcctgataa gttaagtcgt tttcaatcag gtagattagc aatgatgatt gaaggaaact
      961 ggtttgcacc gggatttttg aaaaatgact atctagttga aaatgttgct tctgccgtgt
     1021 tacctaaggg aaaagttaat gcaacaatta caaatggact tggttgggct gccagtgcga
     1081 gtacaaaata cccagaagaa gtgaaaaaac taatggcatt tttagctagt gaggacgcca
     1141 ataaaatcca agcggaaacg ggggcatcaa tcccagcact aaaaggcttt gaagacgagt
     1201 gggctgcttc ttcagataca attgatttga gtgcttttgg aaaaatgttg gagtatggag
     1261 tccctcgtcc atacaataaa gatggattac gagctgaaca agcagaagtt caaattatta
     1321 accgtattct gtctggtgaa gtaactgttg aagatggaac cgctcaaatt gaaaaagaag
     1381 tcaatgcgat attgagcgga aattaaatta gttaattata caatatacag gcgaaaggat
     1441 tctatttcgt ctgtatatta atggatgaat accaaaaagg gggatattat gaaaaatcga
     1501 atttttggaa aatatcgtaa agatcaagtg atatggggct atatttttat ttttccaacg
     1561 gcattaggat taattttatt aaatatatgg ccagcaattc aaacagcaat tttaagtttt
     1621 caaaaaacgg tagggttcgg tgatactgaa tgggtcggat taagaaatta ccaaaaaatg
     1681 tttgcagacg gtgaagtttt tcagtcttta ttaaacacat taatttatgc agtggtttcg
     1741 atacctagta ttgttgtatt atccttgttg gtggcagttt taatgaatag aaaagttaaa
     1801 ggattgtcga tttatcggac aatttatttt ctaccagtcg ttgctgcacc atccgcagtc
     1861 gcaatgattt ggcgctggat gttcaataat gattatggac tgattaataa catgttggct
     1921 aaaataggtc tcgatggacc agcatggtta acggatccat cgattgcgat ttattctatt
     1981 atcattgtag gagtttggag tgctattggt tataacatgg tgttattact agccggctta
     2041 taagaaattc ctaaagatta ttatgaagca gcggaaattg atggtgctag tccaatccag
     2101 cagtttttca acattacgtt accattagtt acaccaacat tattttttgt agttgtaaca
     2161 acagttatca atgctttcca agtatttgat gttattttta tgatgattac tcctaatagt
     2221 accgccatgt ttaaatcaca atcattagcc tatttattct ataaacattc atttatttta
     2281 aatgacaagg gatatggttc ggcaatcgtt atgttccttt tagtgattat attaattatt
     2341 actgcaattc aaatgaagtt gcagaaaaag tgggtaaact attaaggagg ctatcaaatt
     2401 atgaaaaata aatttttttc aaatgcgcca atccatatca tgcttatttt aggtggtatt
     2461 gcaatggcat taccttttat ttggatgatt ttaacttcag ggaaaacgtt ggttgaatct
     2521 acccaaattc cacctacaat tttccctgag aaatttcagc taagtaacta tcaacaagta
     2581 cttgatttat taccattcat ggctttttat atcaatacat ttttaatggt tgttggacga
     2641 gtgattggct cggttttatt ttcagcaatg gctgcatatg cttgtgcgcg tctaagtttt
     2701 ccagggaaaa atatcctttt cagcttagta cttattcaaa tgatgattcc cggttccatt
     2761 tttattactc cacagtattt attagcacaa aaattgggta ttttaaatac aattccagca
     2821 ttaatattac caggggtagt tagtgcgttt ggtacgtttt tattaagaca atttttctta
     2881 ggtttacctg atgaattgga ggaagcggcc tatttagaag gtgccaatat ttggacgatc
     2941 tttttcaaaa ttatgttgcc gcttgctaaa tcaggattgg ttgcacttgg aatattcaca
     3001 gcgttgtttg catttaaaga tttaatgtgg ccattgattg tcaatacttc aattgataag
     3061 atgcctttag ctccgggatt agcaaactta caaggacaat atgccacgaa cttcccacaa
     3121 ttaatggcag gttcgttatt agcaatttgg ccaatagttg tgatattcat cgttttccaa
     3181 agaaaattca ttgaaggaat tgctacaaca ggtagtaaat tataaaacta ttaattagtg
     3241 attggagagt aagtacatga caaaaattac atttattggt gcaggaagta ctatttttgc
     3301 aaaaaacgta ttaggcgatg cgatgttaac tccaagtcta caagatgcac atattgcatt
     3361 gtacgatatt gatgaaaaac gactaaaaga atcaaattta atgcttaaaa atattaataa
     3421 aaattcgaat gaaaatcgtg ctgatattgt ttcttattat gatcgcaaag aagcgttaag
     3481 agaggctgat tttgttatca atgctattca agtgggtggt tataaacctt caacagtgat
     3541 tgattttgat attcctaaaa aatatgggtt gcgacaaact attggtgaca ccatggggat
     3601 tggtgggatt tttagaaccc tacggacact accagtaatg ttagactttg ctaaagaaat
     3661 ggaagaagtt tgtccaaatg cttggttatt gaactataca aatccgatgg cgatgttaac
     3721 catgggtgtt ttaaaagcga cgagtattaa gactgttggt ttatgtcata gcgttcaagt
     3781 atgcgtacca gaattgtttg agcatttagg aatagccgat caatatgact taaatgactt
     3841 tcaatggaaa attgcaggca ttaatcatat ggcattcttg ttggaaatta caaaaaatgg
     3901 ggaagatttc tatccaacaa ttcgtcagtt agctgcagaa aaaccaaatc cacatccaga
     3961 ttctgtacgt tttgaattgt tgaaacgttt tggttactat attacagaat caagtgaaca
     4021 caatgcagaa taccatccat actttattaa atcaaaatac ccagaattga ttgacgaatt
     4081 agggattccg ttggatgaat acttacgtcg ttgtgaacaa caaattaatg attgggaaag
     4141 catgcgagag gacattgtaa ataatgggga gttaacacat acgagaagcc gtgaatatgc
     4201 ttcatatatc atggattcta ttacgacagg aactccgtca gtgattgcag gaaatgtctt
     4261 aaataaaggc ttaatcacaa acttaccgac agatgtttgt gtggaagtac cttgtttagt
     4321 cgataaaaat ggcgtgcaac caacctatgt cggtgaattg ccaacacaat tagctgcttt
     4381 aaatcggacg aatatcaatg ttcaagaact gacagtagaa gccgctttaa cactagataa
     4441 atcaaaaatt tatcaagcag cgtatttaga tccacatcta tcatctgaat tgtctttaac
     4501 agatatcaaa aatttagtgg atgatttaat cgaagcacat ggggactatt tacctgaata
     4561 taattaaaaa gaagctgaaa gagtaaggaa ttttcaattc cttactcttt tcttagttaa
     4621 atttagtaaa aatattgaca aaacacttag taaacagtta cattagtgtt aacaaaaaga
     4681 aaacgcattc aaattgtata aggaggagca taaaatgaag tggtctaaaa aaattatttt
     4741 agcttgtata gcactaggag caacgatctc tttgactgct tgtggaaaaa atcaggaaga
     4801 tgcaagcggt aaggtagaaa tagagttttt taatcaaaaa aaagaaatga ctcaaacgat
     4861 tcaggagatt gctaaagatt ttgaagcaaa aaaccctgat attcatgtaa aagttgtgga
     4921 tgtaccgaat gctggtgaag taattaaaac acgtatgctt gcaggcgatg taccagatgt
     4981 cattaatctt tatcctcaaa gtatagaatt gaaagaatgg gcgaaagctg gttatttaga
     5041 ggacttaacc gaagaaccgt atttagagaa tatcaaaaat ggatatgcac aacgttttgc
     5101 tattgaggat cgtgtatata gcataccatt aaccgctaat gtctatggtt tttactataa
     5161 caaaactgct tttgaggaaa tggggattca agcaccagaa acttgggcag aatttgaaaa
     5221 aatagtagct gagattaaag atcaaaacaa agtaccgttt gcgattgcag gagcagaagg
     5281 ctggacttta aatggttatc accaactagc tatagcgact gctgctggtg gtgaagagga
     5341 agcgaacaat gtttggcgtt tctcagaagt taatggaatt aatgctgaaa gcaaagaaat
     5401 gcaaaaagat tttaatcgat tagatttgtt aagagaacca aaagctttgc aaaacaactg
     5461 gcaaggtgcc ggttataacg atacagtagt tacttttaca aaaggtgatg cgttgattat
     5521 gccgaatggt tcatgggcaa tgccgatgat tcattcacaa aatccagatt ttgaagtagg
     5581 aacttttcct tttccagcag atgaagcagg tcaaagtcta acgattggcg caggagacct
     5641 agccttatca gtttctgcaa cttctcaaca taaagaagaa gccaaacgtt ttgtagaata
     5701 tatgacaaca cctgaagcga tgcagaaata ttatgatgta gatggtgcac cgtgtgctgt
     5761 tgagggcgtt atcgagaata ctcaggattc accattaagt ggtttaactg aacttgcttt
     5821 tactgatcgt catttagttt ggttagccaa agattggaat agtgaaaatg atttttatac
     5881 cttaacaaca aattatttac acaatggcaa tcaaaaagcc atgattaacg cattaaatgc
     5941 tttctttaat ccaatgaaag ctgacggaag gtaggaaaat aaaatgaaaa taaaagaaat
     6001 tttaaatcgt tactgggcaa tcatttttgt agcaattcca attattttgc agatgatttt
     6061 tttctacttt ccaatggttc aaggtgcttt ttacagttta actaattgga ctggtctgac
     6121 ttataatttt gattttgtgg gtttgaacaa ttataagatt cttttttctg atccgaagtt
     6181 tatcaaatcg attggcttca ctttaattgt gacgctttgc atgattgctg gacaaattgg
     6241 ttttggtatt ttgattgcgc gggcgttgaa cgcaaatatt aaagggaaaa cattctttag
     6301 agcatggttc ttttttccag cggtcttatc gggcgtggtt gtttctttag tatttaaaca
     6361 aatttttaac tatggtttac caaaaattgg tagtatgtta ggcattgaat ttttacaaac
     6421 cagtctttta ggaacaacat ggggagcgat ttttgcagta atttttgttt tactttggca
     6481 aggaattgcc atgccagtca tcattttctt agcgggtttt caaagtattc cacaagaaat
     6541 tattgaagct gctagaatgg atggggcgaa tgataagcaa attttttgga atattgaaat
     6601 cacctactta ttaccaagta tttcaatggt atttattatg gcattaaaag gtggcttaac
     6661 agcatttgat caaatttttg ctttaactgg tggtggacca agcgatgcca caacatcttt
     6721 ggggttactt gtttataatt atgcttttaa aaacaatcag tttggttatg cgaatgctat
     6781 tgctttagtc ttatttatct taattgggat tgtttcactg gcacaattaa aattatcgaa
     6841 aaaatttgaa atctaggagg tttcccaatg aaaacatcaa aagaaaaaaa tatttctaaa
     6901 tacttgcttt tattattagg gggcttcatg attttagttc ccttagtttc aacgctattc
     6961 ggttcattta aaacaacacc cgatattatg aataatttct ttcgttttcc taatccagcg
     7021 actttggaaa atttccgaag attgttagcg gatggaattg gtcattactt ttggaattcg
     7081 gcaatcatca caatcattgc agtagttgct gtgacagcaa ttattcctat ggctgcttat
     7141 tcactagcaa gaaaaatgac tcgtgggaaa gcatacggat tgatgtattt atttttaatt
     7201 ttaggaattt ttgttccttt ccaagtaatt atgattccga ttaccgttat gatgagtaaa
     7261 attggtttag caaatatgtg gggattaatc ttactctatc tcacttatgc cgtaccacag
     7321 acactatttt tatatgttgg ttttattaaa ctaaatgtgc ctatcagtct ggatgaggct
     7381 gctgaaattg atggggctag tcactttaca acttatttca aaattgtttt cccaatgtta
     7441 aaaccaatgc atgcgacgac tattattctt aatgctctat ggttttggaa tgatttcatg
     7501 ctaccattat taattttaaa tcgtgattct aatatgtgga cactaccatt gtttcaatat
     7561 aattatacgg gacaatattt taacgactat ggtccaagtt ttgcttccta tacagtagga
     7621 attgtcacga ttacactagt ttatcttgct ttccaaagaa gtattatagc tggaatgagc
     7681 aatggagcag tgaaataacc aataaattat tctcattata aaggagttca taaacttatg
     7741 attatatttg acaatcacac acaaacattt catttacaaa ataaaaaaat tagctatcta
     7801 ttaggaatcg aagaaaataa ttatttaacg catctttatt ttggaaaaaa gattacgcac
     7861 tattctggtg ggtatcgcta cccccgaaca gatcgttcct tctcacccaa caatgcaaat
     7921 gcgcaagatc gtttattatc tttagacaca ttaccattag aatttccagg atatggacat
     7981 ggtgatttta gagaaccggc gcataatatt aaattaggaa acggtagtcg tgtaaatgat
     8041 tttcgttatg atagctacga aattcaaaaa ggaaagccca gcttagcggg attgccagcc
     8101 ttatatacgg aagatgatga tgaagctgaa acgctgatta ttcaattgaa agacgcgatt
     8161 agtggattga ctttacagtt gttctacaca atttatcaaa atcacagtgt aattattcgt
     8221 tctacgcaat tagtaaataa tagtgatgaa acagtggaga ttaaccgctt agctagtcaa
     8281 tcaattgatt ttccaaatcg tccactcgag ttaattcatt taaatggtac ctgggcaaga
     8341 gaacgtcaaa tgactagaga aaaaattcat acagggataa aagtattaga tagtaaacgc
     8401 ggatcaagca gccatcaaca aaatccgttt gttgctttag tagatgccaa aacagatgaa
     8461 tttcaaggag aggcatatgg atttaattta gtttatagcg gtaatcacga aacggtgatt
     8521 caaaaagatc catttgacca aacacgggtc attatgggaa ttaattcatt taattttggt
     8581 tggcaactaa aaactggcga ctcatttcaa acgccagaag tcgtgatggt ttatagtaat
     8641 gaaggtctga atacgttatc acagacgtat catgaattgt ataatcaccg tttaattcgt
     8701 ggaaactatc gtttaaaaga acgtcccacg ttaatcaaca actgggaagc aacgtatttt
     8761 gattttaacg aagaaaaaat catgaatatt gtcgatgatg cgaagaactt gggcattgaa
     8821 atgtttgttt tagatgatgg ttggtttggc ggtcgtgaaa atgacttcac ttcattaggt
     8881 gattgggttg aaacagaagg caaacttgct tgtggtttgg aaaatttagc caagcaagtg
     8941 catgataacg ggatgaaatt tgggatttgg tttgaaccgg aaatgatttc tgaagatagt
     9001 gaactatttc gagcacatcc agattgggca ttgggtattc caggtcgtga ccgctcaatt
     9061 agtcgaaatc aatatgttct tgatttttca agacaagatg ttcgtgataa tatctatcaa
     9121 caaatgacgg cgatttttga tcgtgtaccg attgattatg tgaagtggga tatgaatcga
     9181 aatatgacag aagtctattc tgtcttgttg gactcaacga tgcaaggtga agtggctcat
     9241 cgttatatgt tagggttata tgaattttta gaaaagttaa caacgaatta tccaactatt
     9301 ttatttgaaa gttgttcggg tggaggcggt cgttttgatg caggtttctt gtattacatg
     9361 ccacaaactt ggacgagcga caataccgat gccgtggctc gtctgaaaat tcaatatgga
     9421 accagcttag tgtatccaat ttcaagtatg ggtgcgcatg tttcagcagt cccaaatcat
     9481 caaacaggaa gagaaacgag tttaactatt cgtggcaatg ctgcgatggc tggtgttttt
     9541 ggttatgagc tagatctaac gcaattaact tctgaagaaa aagcagaaat taaagaacaa
     9601 gttgcctttt ataaaaagca tcgccaactt ttgcaatatg gaacttttta tcgcttggaa
     9661 agtccgtttg aaggcaatga tactgcatgg atgtttgtct caaaagacca aacagaagcc
     9721 atggtctttt atttccgagt attagcagaa gctgcgcatc cattagtcac attgaaatta
     9781 gcaggattag cagaagaagc aacttatcaa atggaagatg taacatttgc tggcgatgaa
     9841 ttgatgaatc tcggttttta tattgactca gaactacatg gtgattatgc gacacaacgc
     9901 ttttatttga aacagatata gtagcttgaa tttacaatat aagtgcaaca ccaaaagaga
     9961 ctcattccaa acgaagaaaa tatgttttat ttacacgcat ttactaaaaa gcagcctgat
    10021 ttgaattggg aaaatgaaga agtacgtgaa gaaatctatg caatgattaa ctattggtta
    10081 gataaaggac taggcggatt tagaatcgat gcgatattaa acatcaaaaa aaaactagag
    10141 tatggcatct ttgaatctga tggagaagat gggttagcct tcattgggcc ctggattctt
    10201 aatcagtcgg gcattgaagt gtggttaaaa gaaatgagag agcggacatt taaaccacat
    10261 aatagtatga cggtagcaga agccgatgtt cctaatgaac gtttagatga atatattggt
    10321 gaagatggct attattcgat ggtttttgat tttagttata cggatattga tgtacctgaa
    10381 actggtgaat ggtttaaaga tagccaatgg acatggacgg atatgcgtac caatattttt
    10441 acgaatcaat tagttacgca agataaaggt tggggcgcct tatatttaga aaaccacgat
    10501 caaccacgtt caattaataa atatattcca gaagaatgga tcaatgatta tagtaaaaaa
    10561 atgttggcga cgctttttat gttactaaga ggtacccctt ttatttatca aggacaagaa
    10621 ctaggaatga ccaacataga aatggcatca ttagaagatt ttgatgatgt agctacgcat
    10681 tctcagtata aacgggcatt agaatatgga ttggcaccag agcaagcatt gaaagctgtc
    10741 aataaacgaa gtcgggataa ttctcgcaca cctatgcaat ggaatacgca aaaaaatgcc
    10801 agattctcaa cttctgataa tgtatggtta aaagtaaatc ctaattatcc agaactcaat
    10861 gcagaagctc agttaaccaa tcaacagtcg gtattcaatt attatcatca attaattaaa
    10921 ttaagaaaaa atagtgtata caaagacgtg ctaatctacg gacaatttct accagtgaaa
    10981 gataaaaatg aacatatttt gctttatgaa cgccaattag aagataaaat cgtattagtt
    11041 atcttaaacg ttacagcaac agaacaagaa tattatgtag atgaacagta ccaacaagta
    11101 ttaatcaata attatgaaga tgttttatta gatcaaagtc gaaaattgcg tttacgaccg
    11161 tttgaaagta ttgtattagc taattatgga ggacaattat gaaaattaaa aacgaagcaa
    11221 tgctcatcac ctatgcagat agtttaggaa aaaacatggg cgaattaaaa caagtcttag
    11281 acaaacattt acaaggtgtt attggtggtg tacatctgtt gccttttttt ccatcgacag
    11341 gtgatcgagg ctttgcgccg agtgattata cgacagttga tccagctctt ggaacatggg
    11401 aagatgtgga agcattaggt gaagattatt atttgatgtt tgattttatg attaatcaca
    11461 tttctcgtga atcaaaattt ttccaagatt tcaaaaaaaa ccatgagcag tcaccataca
    11521 aagaaatgtt tatccgtatt catgaatttt tcccagaaaa tcgaccaaca caagaagata
    11581 ttgatttaat ctacaagcgt aaagacaaag cgccgtttca aacggtagaa tttgcggatg
    11641 gtacgacaga agaggtttgg aatacctttg gggaagaaca aattgattta gatgtgacca
    11701 aagaagttgt gaaagaattt attcgagaaa ccatcaaaga tatggcgaac catggctgtt
    11761 ctttaattcg attagatgcg tttgcttatg cgattaaaaa attagatacc aatgatttct
    11821 tcgtggaacc agatatttgg gaacttttag acgaagtgcg tcaagaagca gcgaaatatg
    11881 acgtcgaatt attaccagaa atccatgagc attattccat tcaaatgaaa attgccaatc
    11941 atgattatta tatctatgac ttcgccttgc caatggtcac tctgtattca ctttatagtg
    12001 gaaaatcaga acgtttagcc aattggttga aaatgagtcc gatgaagcag tttacaacat
    12061 tggatacgca tgatggaatt ggtgttgtgg acgctcgtga tttattaaca gatgaagaat
    12121 tggattacac ctctgaggaa ttgtataaag tcggggcgaa tgtgaagaag gtctattcaa
    12181 gtgccaatta caataattta gatatttatc aaattaacag cacctattat agtgcattag
    12241 gcgataatga ccgtagctat ctgttagcac gtgcgattca agtatttgcg ccagggattc
    12301 cacaaattta ttacgttggg ttattagcag gaaaaaatga tattgaactg ttggaagcaa
    12361 cgaaagaagg gcgtaatatt aatcgtcatt actatgattt agccgaaatt gaagaagaag
    12421 tgcaacggcc agtgattcaa caattatttg cacttttaaa atttagaaat gaatctgcgg
    12481 cgtttgattt ggatggaacg attgaagtag caacgccatc agaatcagaa atagtgatta
    12541 cacgtaaaaa taaaaacggt agtgatagtg caacattaac ggctaacttg gatacgaaag
    12601 aatttatcat tgagtctaaa gatataaaaa ttttcactag tgttgcatta aaattttaac
    12661 ctattccata gaacaaaaaa tcctttatac tagcacttgg atgacttatc caagaccaat
    12721 aaaaaggact catacatgga taagtataaa acaaatacag catttaataa atggttttct
    12781 gccataaaat taggaaaact ttctgtcaac tcccaaaaat gtatttttga ttacaattcg
    12841 tatcgtaaaa aactgtcttt tgaagctgtg ctcaagctct ttctctacgc agttaatgaa
    12901 gaaaaagaaa gtttgcgtga tttgagtaca gctcttatcg accgatcgct ccaactagaa
    12961 accgatgtgt catcgattag tcatacccaa ttgtctcgag catttaatgc acttgaacct
    13021 aaagtgttag aagaaatttt tcaacaactg ttagaaaaag ttcaacatca aatcaaacca
    13081 accaagcgaa atcatctgta tctgattgat tccagtacgt tttcgcttag tttaaaacgt
    13141 cataagtggg cgactttccg tcaaacaaaa tcaggtgtca agttacactt aaactattgt
    13201 tatatggacg atactacgat gtatccaact gattttacct taacaaatgc ccatgaacat
    13261 gatctcaatc aactaccagt attggtgaat caaccagagg caacttacgt gtttgatcgt
    13321 gggtattagg actttgaaaa gatggatcag atgcattggg atggctattt cttcgtcact
    13381 cggattaaaa aaaacaccaa agttcacgtg atagatactc tggaaacatc gccagaaaca
    13441 gaaattctac gagacgagct ggttcgttta ggatctaaaa cctatctaac cgccaatttc
    13501 cgactagtca ctgttcaaga taagaacgga cgtgtgtttc aatttatcac caatcgaatg
    13561 gacgtctctt caaaagaaat ctctgatatg tatcacgctc gttggcaaat cgaattgttt
    13621 ttcaagcaca tcaaacaaca catgaccatt aaaaccttct tttctcaaag tgaaaaaggc
    13681 gtgcagaatc aattgatttt aacgatgatt tccgctctcc tcacgtttct catcaaattg
    13741 gagacgaaaa cggaaaaatc agtgttccaa atcaaacgat tctttcgtta tttactgttt
    13801 cagccctttg aatgttgtct agaaaaatta attcccacat gagaaaaaag aaatttggct
    13861 cgttactttt ggttttcagt tgaataacag ataaaaaatt ttctggaaaa agtcatcctc
    13921 tgcgtcagca cagttttttt tgacttattt tcagaggaaa tagttttcag aatattctaa
    13981 aatgttttat gcaacactag tgatattttt tagaattaga tttctaatgt gcattttttg
    14041 agtattaagg agcaagtata caaagattta taaagctgat taaatttgaa taaaaatatg
    14101 caggaacgct actattttta gtagtgttct tttttatatt tgaataaaaa atgtggtggt
    14161 taaatggtgg ttataacaaa aaatgaatta atgaaattag gttatgggaa gtatcagtct
    14221 gagagaatca tacgacaggc aaaagcaatt atggtttcaa aaagctatac ctattacgaa
    14281 aatagaagat taggactagt acctgttgaa tctgtagaag aagttttagg tatgaagata
    14341 gaggtagagg aggattttca gaatcatggt taattttaaa aaagataaaa atgggaaata
    14401 tcattttact gctagtttgg gattcgacaa acgaacaggg aaaagagtac aaaaaaacgt
    14461 agaggggtta aaacagttaa agaagaaaaa aagcgtatac cgaacttgtt tctaattatg
    14521 gaaaagaagc agaaacttct caaagtacta tgttgtttga ggagtttttt tataaattat
    14581 tcctacctta ttatcaaggg aaagtagaag aaagaacata taaaaatcgg ttatctacaa
    14641 tcaaaaaaac ttttcttatt tctttaaaat ggagttaaga gaaatacgtc caattgacat
    14701 tcaaaagtgg caaaataatt tattgagcaa atataaaaat tcttatgtaa gaaacatgta
    14761 tgggattttt cagatgtcat tagatcgagc agttgttttg ggactacttg aaaaaaatcc
    14821 tgcaaaagtt attggaaatg tgaagaaaag gaaaactaca gttgattttt ggacaaaaga
    14881 agaatttaca aaagttattt caacttttta tattgatgat ttgtaccaac attttggttt
    14941 tatttgtatg tggttacttt ttatgaccgt tatgcgtttg ggagaagcca ctgcattaac
    15001 ttggaaagat attgattttg aaaataaaaa cttgcgtgtg aataaaacac tctattataa
    15061 gagagccaaa acctatgagt ttgtagaacc cgaaacggct tctagtattc gtacaatcga
    15121 actagacgac aatacaatta cctttttaaa agcttggaag gagcgtcaag tttcaatagg
    15181 aggcatgtcg tttgtacttt cttataatgg aacacctact cagaaatata caatccgtca
    15241 tattatgaga agacatgcaa cgatcgttgg tgttcatata atccgcatac acggattaag
    15301 gcatagtcat gcttctttgc ttatatcaca ataaaaagat gcacttcgaa ttaaagaaag
    15361 gttaaaacat gctgatgtac aaacgacttt aggaacttat ggtcatttgt atctaaattc
    15421 attgagagag ataacaaatg atcccacaga ttatatccaa actaattttt ctaaaatcaa
    15481 accgacacct atttacgata ataaaaaata aatgataaac aaaagtagga tcacaccaaa
    15541 agaaacacac tagaaaaact ataatacagg cttttctagc gtgttgtttc ttatttccgc
    15601 ctatttatac attatactca taaagcattt aatgcatcga tcgtagccgc attgtctata
    15661 tgggtatata aagttgttgt attggtactt gaatgtccca attgttgggc agttaatacc
    15721 aatgaatttg tctgttggta gagtctagta gctaatgtat gacgcagttt gtgagggctg
    15781 acttgaattc taaagccttt tgaatatttt gctaccatcc gttcgattgc aataccggat
    15841 atacgcttgg cttctccttg gtaaattgct agaaataaag ctttttcgtt ttctgttact
    15901 ttatatcgtt ctttccgaat agctagataa ttagctaaat attccattgc gaatgctgct
    15961 atattgacag catctctttt atttcctttt ctagttacta ccacattatt cttatctaaa
    16021 gataggtcat ctaaattaat atttataacc tcggagagtc tcaatccaga tcctaaaatc
    16081 agtgcattga tcgccaggtc tctttctaca tctcgttgat aatagatcaa agctcgttta
    16141 ctcaaagtat ttttatattc attggcaata taatctagat aattttgtgt atccttatct
    16201 agaaataatt ttttctcaat ggcattggca cgtgcagcta acgtagtttt atctgtgatg
    16261 gttgctactt gatccatagg attattgtct aagtatggtt ttcctagagg attattggaa
    16321 cttttcgaaa gatatttaaa tagaaccttt aatgccgtga tatttctatt gatagtagaa
    16381 gtagatagac ctaagttatt attatctttg agcttagttt ttgttgtctc tttttttaca
    16441 ttttcttttg tgcgacgttt caaatgagat ttgtacaagt ttaggtcata ggcagttaac
    16501 tgtcttatcg aggtgataga agtatcagga acgatagact cactcagaag ccattcataa
    16561 aagagcttat aattttttag atattctaaa gtggttcgag gtgattgatc tttatcctca
    16621 cgatcttgaa caaaatcttt tatataatcg ggcatatgaa gtatgattgt atctattttt
    16681 tgatatattt ctgtattttt catgtcattc atcctttcaa taagtagggg agaagagtat
    16741 ctctataatt ggactatacc aattatagaa tgttttgtca attggtatgc tctaaaattt
    16801 attaatttgt ttcaaaaaaa gcatataaac aggtatacgt ttaataattt aaaataaatg
    16861 gtatacataa atagggaagg aaatagaagg gagcaatcta aattttttga attttcttaa
    16921 ttaattatat agaaagaaca atctttctat agagttagac atgagaggga ctatgcttga
    16981 tttaaaatca acaagcgtat atactgtata tacaaagaaa aaggagggag aagactatgg
    17041 caattaaaga aagaaaattt agggaagtgg ggaactcagt agtattaact atttcaaagg
    17101 agtttcttaa agaaaatggg ttaaatcccg gagatacggt ttttttagat gaagaaaaac
    17161 ttcgagcggc aattacaaaa aaacaagcct tatctgatga aattgattta gcaatatctc
    17221 aagcattaac tgaatatgat actactttta gagagcttgt cgataaatga gtattcatta
    17281 cctagaagcc aaagatatta taaaaatgaa tgtaatccaa ataaaaaaat actctcctaa
    17341 tgaacccctt ggtgtaaagg atgcgaatgc tttagaaatg tgtgtagggc aacttcgagc
    17401 ctcagctttt ggtgaagagg tttatccttc gatttatgaa aaagcagcta tcctacttat
    17461 acaattgata aagaaacatc cttttcacaa tgcaaataaa cgtactgctt tcttagctac
    17521 ttttgtcttt ttgaaaatca atggacagct tttagctatt gatcaaaaag atgctgtaaa
    17581 tttagcggta tatattgcta cgtatgataa ggatttcgat caactaaaat atgaagtgat
    17641 caacactata aagacacata cgcagcccat ttaaggagtt actgtatgtg tctttatagg
    17701 gttattagct gctatagcga aataattgaa gtaaattaca aatagctata attcaaatcc
    17761 aactatatag aaaacgtaaa ttttctatat agttggatta agataacgac tatttatttt
    17821 atcatatttt tactataccc tatactttta cttggtatat ttatgaataa tcgcaaattt
    17881 acccctatac tactagtttg taagttagat taataaaaat actaaagtta tttgttcttt
    17941 tattaaatac acctacaact tataaattga tggtatttag ataacacgaa agaataaaaa
    18001 taacagctga tagtaaagtt tgttgacgat tgctactcaa tcgaaagtga gttagtgcgt
    18061 atgaacttgc ttctgaaaac ttatgaaatg aaagaccttt tacatcttgc agaaatcggt
    18121 actgggatta aactaagcaa taaaaactaa tgttcccctc attctttgaa cttctactat
    18181 ttgatttatt tattctgata aatgacgatt tcaccttcag gatcttcaaa tcccgtatag
    18241 aatttattta agatctcttc agtatcagta ataggatagg cgataggcca catacccttt
    18301 gatggttaga ataaactgct caaaatctgt atcgtatgtt ggctttagca tattaacaaa
    18361 ggctatctcc aaattataaa ccataagcgt tgaatcgttc ttaacaattt caattcttcc
    18421 taacccaaag tcagaagata taattaccgg acttgcctta cccttcatgt gggttgttga
    18481 gattccatat ttaaagggaa ttgtcggtac tatcggcggt ctgaaaaaca tcccaaaaat
    18541 aaaaaaatct ttcccctgct agtctcctaa gtccctttgt agcggatatg gcaccataat
    18601 ctcattttat cgaagtgagt catggtttat ctgttggcca accaaaattg atacaatcta
    18661 acgatgcacc ctctacggca ttctagggga gtgcacttgc attcacccaa taaaaaccgg
    18721 cagttagcaa agaacttatg tagtcgtatg ttcttactgt taacatacaa gaggtctcct
    18781 ttccacaatt gcttagcagt aaacgggaat ttacaaagct aatctcttcc attttcttat
    18841 ctttgttcta aaagtcccaa atggagcaac tgtatttaca tgaataaatt tatatacttc
    18901 ccatacagcc gttttggttg cctcgtctgc ccattttctt ttgtgtgcag tgaatagatc
    18961 ttcatcactc attgaatcaa tcatcatgtt aatatcatca atattacgat tgagaatttc
    19021 ctctaattct ttcaatggta gatgtgcata ggtatttgta aaccacttat ataactctcc
    19081 taactgattc catttaaatt gatcagaagg cgttttaaca gataatccat ttctttcatc
    19141 attttcccat tttagaatta aattcgtcca accaacttga tatgctaaat tttcagcagg
    19201 tgttctatcc acttcctcaa ctcttttatc cttcaattct tcaggaatag tttcaaattc
    19261 taaaatatat ttagtataag tcctatttat ttcttttttt aattcttctt tatttttata
    19321 tgttctcatt atattacctc cataaattcc taattgtctc ctttaattca tctataattt
    19381 catccacgat attttaatcc gttctacaat gcatatctga tatgcattga ctgtctaact
    19441 catatgtgaa tgatttataa aatatcccaa cgtctcatct aaagaaatgt tgatcgtcag
    19501 caaggagcta aaaaatacga atatatttct tttgtagtaa aatcggactg gtttgcatat
    19561 cattttcttc gtaaatatca aaatcatgtt aactactatc aattactaat aaaaactacc
    19621 actttaagcg ttaagaactg ttaagtttag ttaagtgagg atagttcaca aaatccattg
    19681 ttaaacttca cgaaagtggt aaaactcaaa atgctttata taggattaca gcatcgctct
    19741 ttcttcgatt gcgcgttggg taaaaaatat tatgaagtaa aaattgacga tgacacgttc
    19801 cttacggctc atcagatgaa acagctacaa aaacgaaatg atcaactcga agaaaattta
    19861 attctaaaaa agcgattgcc atattcacgc ctcgctcgac gaactattaa aggtcaagat
    19921 ttacctgaag atcatccttt ttaggaaatt gaagcttttt atgttgcaca atgcatttat
    19981 aatactacaa tgatgttttc accagatatt cttctagggt gcggtgtaat gaaacagata
    20041 cacttgcaaa agaaaattca ccgcgaattt actaaaatta tgaataatta tgttaatctt
    20101 ccgaaatttt tttccaaaat taagggatca agctggtgtt atagattgtt taaggttggc
    20161 aaaaaaataa cttcttttta aacaattata gaaaaccgat actctgttaa gttggttgac
    20221 acggtgtcga tttttatgac tttcttattc tgaaacttca ctaacggaca gtacctaaaa
    20281 agtctatagt atggacgttt acgagtacta ttttatcaaa aatgctgctc atttcaactt
    20341 atatgtttta gtgaactacc cccactta
//
LOCUS       DACDTO010000048        20289 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDTO010000048 DACDTO010000000
VERSION     DACDTO010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20289
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.048"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(110..1573)
                     /locus_tag="IWK70_001736"
     CDS             complement(110..1573)
                     /locus_tag="IWK70_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9614265.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(1637..4654)
                     /locus_tag="IWK70_001737"
     CDS             complement(1637..4654)
                     /locus_tag="IWK70_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9614266.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(4805..5644)
                     /locus_tag="IWK70_001738"
     CDS             complement(4805..5644)
                     /locus_tag="IWK70_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9614267.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            5789..6034
                     /locus_tag="IWK70_001739"
     CDS             5789..6034
                     /locus_tag="IWK70_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614268.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            6097..6519
                     /locus_tag="IWK70_001740"
     CDS             6097..6519
                     /locus_tag="IWK70_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614269.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(6613..6930)
                     /locus_tag="IWK70_001741"
     CDS             complement(6613..6930)
                     /locus_tag="IWK70_001741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614270.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(6957..7163)
                     /locus_tag="IWK70_001742"
     CDS             complement(6957..7163)
                     /locus_tag="IWK70_001742"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614271.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(7144..7326)
                     /locus_tag="IWK70_001743"
     CDS             complement(7144..7326)
                     /locus_tag="IWK70_001743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614272.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(7340..7690)
                     /locus_tag="IWK70_001744"
     CDS             complement(7340..7690)
                     /locus_tag="IWK70_001744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614273.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(7716..8018)
                     /locus_tag="IWK70_001745"
     CDS             complement(7716..8018)
                     /locus_tag="IWK70_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614274.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(8021..8254)
                     /locus_tag="IWK70_001746"
     CDS             complement(8021..8254)
                     /locus_tag="IWK70_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614275.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(9240..9562)
                     /locus_tag="IWK70_001747"
                     /pseudo
     CDS             complement(9240..9562)
                     /locus_tag="IWK70_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9685..10800)
                     /locus_tag="IWK70_001748"
     CDS             complement(9685..10800)
                     /locus_tag="IWK70_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614276.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(10806..11204)
                     /locus_tag="IWK70_001749"
     CDS             complement(10806..11204)
                     /locus_tag="IWK70_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614277.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(12233..12619)
                     /locus_tag="IWK70_001750"
     CDS             complement(12233..12619)
                     /locus_tag="IWK70_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9614278.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(12616..13137)
                     /locus_tag="IWK70_001751"
     CDS             complement(12616..13137)
                     /locus_tag="IWK70_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9614279.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(13139..14173)
                     /gene="sppA"
                     /locus_tag="IWK70_001752"
     CDS             complement(13139..14173)
                     /gene="sppA"
                     /locus_tag="IWK70_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9614280.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(14206..15621)
                     /locus_tag="IWK70_001753"
     CDS             complement(14206..15621)
                     /locus_tag="IWK70_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP9614281.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(15590..17743)
                     /locus_tag="IWK70_001754"
     CDS             complement(15590..17743)
                     /locus_tag="IWK70_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP9614282.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            18001..18237
                     /locus_tag="IWK70_001755"
     CDS             18001..18237
                     /locus_tag="IWK70_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9614283.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            18256..18471
                     /locus_tag="IWK70_001756"
     CDS             18256..18471
                     /locus_tag="IWK70_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9614284.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(19174..19926)
                     /locus_tag="IWK70_001757"
     CDS             complement(19174..19926)
                     /locus_tag="IWK70_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614285.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(19941..>20289)
                     /locus_tag="IWK70_001758"
     CDS             complement(19941..>20289)
                     /locus_tag="IWK70_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9614286.1"
                     /translation="CPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQDRELSLAIYR
                     DDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKKNPNMFVNYQN
                     DPYQFTTQVILLF"
BASE COUNT         5870 a         3931 c         3034 g         7454 t
ORIGIN      
        1 gatacgagat ttcaaattaa aaaacgaaaa tgagagatac aaatatatca aatatttaaa
       61 aagtaaccac attttctata ccactcctta tcagaaacag ccttattctt tagaataaga
      121 ctgtttctgg gttgattaat ttgtcttttg ttaaactacc atctaaggca agttctaaat
      181 gtaaatgtgg tcctgtacta tttcctgttg aacccacata accaataatt tgtccttgtt
      241 caactgtttg tcctacgttc acgcaatatt cctgttggtg cgcataaaga gccacacttc
      301 cgtcttcatg ctcaattgtg acataatttc ctgacaaagt atgatacgaa gcttctatga
      361 ccgttcctgc cttgctcgca tagattggtt ctccttgatt ggctgccatg tctaatcctc
      421 tatgaaattc ttctcctcgc caaccgaagg aactagaaat tgaataattt tttagaggta
      481 ccatatagcc ttctttgctt agttgtggac cagctacttc tttatcatat ttgattaagt
      541 cataggtttc gattaaccca tttaattttt tatcgtagct agtgtctgtt gcgtatcttc
      601 ctgttaaaaa tttagtcgct tcctgatagg tttttgcctt acttttccaa acaccatcat
      661 aaaaagaggt atttccaact aagccttctt ttaacagttt ggcatagtct tttaagcttt
      721 cttcatagct ctcatattgc cgaaaagttg cttgggtcgt ataaagtgtc ccattgccta
      781 aatcttcttg tgtcgcaaaa gagacgccct ttccgttatg tgtgcctttg attccaaata
      841 gattgtagtt tggtgcctga gccaattgac tttgtccgct tgcggattct aaaatggctt
      901 gcgcgatcat aacagaagcg tataaatcat tttcttgtcc aattttacga gcagattcac
      961 cgattttacg gataaacgtt tctacagacg cgtctttttc aaaatgaata gtcccttcgt
     1021 tatcttgtgt gctggaaggg actaattctt gtatcggtgc gcttggtgct tggtggtagt
     1081 tttttgtctc ttctgaaccg attgattgtg cctgcggtac gtcattcata ggtacagtca
     1141 cactctgttc aggtgccttt tgtgggcgtt ccgtttttgt tggcggttcc gcttctgttt
     1201 cggtacttgg tttttgagga gtctgtgagc tttcatcaga aggttgctca tttggttctt
     1261 ctgacggttc ctcctcaggt tgttgattgt cttctggttt gtctggttga ttcggtgttt
     1321 ctggttgttc ttcagaacct gttggtggag cgttcatttc tggattatct ggtgtatcta
     1381 ttgtatcttc agtcggtgtt tctggttgat tttcttctcc agtggaattt gttgtcgttt
     1441 cttcagtgat cgttgcagat tgcgaatttg tcagttgatt gttgccttgt tcattagcca
     1501 aaaccgctgg cgtataccca aatacttgac ctactaataa caaagcagat aaacatttac
     1561 tcgttttttt cacaagattg cctccattct tcaatcaatc atctagagac agctgattgc
     1621 tgtctctagt catactttaa ttgctttgtt tcattttacg acgaaccaga acagccccca
     1681 caccaacaaa aagaaacagt cccaatgcaa ttaacacatc gttcactgtt tctcctgttt
     1741 ttggtaagct ttgttttggt gtgctcgtcg gcgtttgttt gacaggggtt gtcggcgttt
     1801 cttttttaat gccttttgct gtcaatgttt gtttttcatt cttcaaatca aagtgacgtg
     1861 cttcttcctc tttatggtct ttatccttat agacaatatg agttgcgacc aatttctccc
     1921 cgtcttttaa cgtgcctgct ttgtagtcaa aggatacttt aaactcttca gacgctttag
     1981 aaactttacg tgcagtttct accgtaccaa cgactgtatc ttttccattt ttatcaatct
     2041 tatggaattg agtcacccag tatttggttt cccctaccgt agattctgga aattcttgcg
     2101 atacatgatc gatcaactct tggtctttgt ctggatcaaa tgtttggtcg ccattcactg
     2161 taataaagcg tgtttcgatt tttggttctt tcgcagtcac tgtttgatct ttattggtta
     2221 aatcaaaatg acgagcgaat tcttcttcct cttctttgtc gttatagaca atgtgcgttg
     2281 cgacaatcgt gtcaccgtct gaaaccatgt ctgctttgta gttgaacgtc acatcaaatg
     2341 tttctttttc atcggtcact tttcgtgttt cttttaccgt atctaataca gtgacttttc
     2401 catcggcagt aattttatgg aattgcgtca cccaatattt ctctgtccca accgcagatt
     2461 tagggaatac ttgggacact ttatccacta attcttggtc ttttttcggg ttgaaatctt
     2521 ttccacctgt cgttgtcaca aacaaagtag aaatttctgg ttggtcttct tctggaacca
     2581 cgatttcttc gtttttcaca gtaattggtt gtacttctcc gtctttgacc gttactaaaa
     2641 tcgtttctgt ttgtcctgac gcttgcccat aatgctcagg gaacacaatt tccgtaaatt
     2701 tatacgtacc gtctggtaat tttagttttt ctggttcttc ttttgtttca tacacaaagg
     2761 acacattttt attatgtgca gtcatacctt caatcaggaa tttcgctcct ggtaattgta
     2821 gatgattgat tgaatcgact ttattgaagt ctacttcacc cgtttttaac aagttgtcaa
     2881 tcacatctaa ttcaaacgta agcacaggcg tcgtgttatc gccataggcc acagagaacg
     2941 tggtttgttc ttcagaaggt tgacggtagt tgtctggcac atttttttca atcaattggt
     3001 atttgttttg atcgccattg tagaattttt tgtttgcctt ccagtgtccg tctttgtcag
     3061 tttttactga atccaagact tctccttttt tcacaaggac tgtttcttta tctggcgcag
     3121 taatatctgt tttggcgata atatcaaatt cgatattttc tgcaggaacg gtatcataaa
     3181 caggtttgcc ttgattccag gattcttccg cattatagac ttctttgact ttctttcctt
     3241 ctggaatgaa ctgttgtaag tcattttttg cagttaacgt cacttctttg tttggttcaa
     3301 tcgtaatcgt atgagtttgt ttgtctagaa tatacccagc tggtgctttg acttctttga
     3361 catccaattt attttgaaca tattttccgt ctttttcata gaatgcaggg atttctttta
     3421 aggcgatttc ccctttggca tccgttgtat attgttttgt ttctcccgta tcatgacgtt
     3481 tgatttcata cgtcacattt ggaacttttt tccctgtgac agcgtctagt ttggttagtt
     3541 tgaagtcgcc attttttaga actttaatag ttagtccagc tctattagga tctggcacat
     3601 aaagctttcc tagattttga gcaacaggat ctacataggc aaaacttgaa cctacccctc
     3661 tagaatttgg atccagtgaa gaaacagaaa ttcgtccgtc atttgagttt tgatttgctt
     3721 ttataaacaa actatttcct gattttctta cttgaacgtt agctgtgttt tcaacaaccg
     3781 acccatccca ttgagaaaac acaccttttg tatccttaac ttctacttct tcccctactt
     3841 taaccgtcac tgtttggcta tcaaaagaag gatcttcaag taaattgttt atttttctat
     3901 ctacagtctg tttgaaagct tcataattag attgtccaat agttccataa ttaacaaatg
     3961 aaccacctaa aacttcccaa atatatgttt gtgctaatac atagttatcc attgaaccac
     4021 tagaatagta gccaaaataa ccaattaaag aaatcttttt tctaatagca cttgtcgtaa
     4081 ttaaattttc gagtggtgtc acatcatatt gaatacccgc actcgtgatt gcgccttctt
     4141 gaatgcaaaa aacatcttct ccgtcaattt gccagcgaac catgtaatca tgagtataca
     4201 ttgttccttc aggagtaatt cgagtcatat tcacatcttt acgaccattt tcagtcaatg
     4261 ttagattggt agcacgtgtc tgtgcattct tttccaagat tgcttgccat tcttcagatt
     4321 cttgcgccaa tcgttcttgt tcagaaaaag aagtcgcttt ttgaatatct tcaatggtta
     4381 taaagtattt ctcataacct tcagggacct cttgagtcat tacattgggc ggagaaacat
     4441 cttctgtcgt gtcctgcgta gttgtctctt ctgttgtttc acttgtggtt tcagtactga
     4501 tcgcttcttc actacttttt gattcttcag tcttcattac ttcttcagta gaatttgatt
     4561 catttatcgt ttctgcaaat gccgttacag atcctaacat gggattgaga aatgtaacga
     4621 aaattgccaa ccaagttgcc attcttttaa acatattttt ttcgttcctt tcattttatt
     4681 tttcagtctt ctaaagtaat ttcttatgga cttcgacacc gaaacatttt tatcctcctc
     4741 aaaacaaaaa aagactagaa cttataattc tagtcttcat caaccactta cttttctttt
     4801 atttttagta gaaatctaca gtgtgttttt gtgacccgtc actgtaaata ataacatcca
     4861 ccataaaacc tttgttgtac cactgcgaat ttggatcgct aacctctgca ttagcccaat
     4921 cataggcttc atctacagtg ttaaatacct ttccactatt accaatctct ccaactacaa
     4981 ttgttggctc ttcaggtgtc gttgtgccac catttccgcc ttgatcgcct ccaccaggat
     5041 tgcttggttc ttcaggcgtt gttgttcctc cacctgtgct tgggttagaa ggagttgttt
     5101 ctcctccagt atttccacca ttacctggtt gtgtaggtgt ttgagtagtg ccaccattgt
     5161 tttctggtgt acttggcgtt gttgtaccac cagttgttcc tgtttgatct ccagctggtg
     5221 ttgttggtgt cgttgtattt ggtgtcgttt gtgtatttga agtcgttcct tgtcctactg
     5281 gttgattcgg atcaactttg tctttctctt gaatcactgt ttgagccaca gtttcacctg
     5341 cgctgttttg tacacttgct acattgccat taaatactaa tttatttcct gcatagatta
     5401 aatccacatt tgtaatctta ttcatgtccg ctaatttttg gacagtaata tttgttgctt
     5461 gactgattgc gcttagggta tctccccaga caattgtgta ttcattccct ttaatttctg
     5521 ctttgatttg ttccgccgaa cgaggtgtcc aaactttgtt ttctgctgct tcagcattcg
     5581 ttcccacagc aagactaact cctccgacta cgattcctag tgccaatact ttcaacattt
     5641 tcattgtttt tcgcctccga tttgaataaa tttatttaca aaaacatagt accaaactta
     5701 tgtctatcag tcgatatatt ttttgtagat ttttttattt tttgatatac taactttggt
     5761 actaaaataa ctaaaaggaa tgtcacacat gcaacttaat aatttaaatg aaatacatga
     5821 ttgggctcaa aataatttac ttaatcgtaa acaagctgcc aaaatcactg gacaaaacta
     5881 tgatgctttt tctagctctt tacgtagaaa taatatcaaa ccatttctag aaataggcga
     5941 cgaacctggt ccttccatca ttcgcttata tctaagagaa gatattgaaa aatacgcaaa
     6001 acagctcgaa gaaaaaagac agaaacgtca atgaggcgtt ttctgtcttg gatttacaag
     6061 taaaacaata ataaacactt ttacaacgga ggttctatga ataagaaata cattgtttca
     6121 gttggttcaa tcgtactgat tgtactcgct attattgctt tttctgtatc caacaaaaca
     6181 gaaacacagc cacaaaatac ggttcaatcg actactacaa gtcaatctag cgatcattcc
     6241 gtctatcctt cactaaatag tttaaaagaa acgcatggac aagatttaat tgatcttggt
     6301 tcttctcaaa ttactactgt cttatttgaa aagaaaaata ttgcgtttat tactcctaaa
     6361 gaaggaactg accttgaatt ttctatcaaa gtaacaaata ccaaagaaaa tttattctcc
     6421 attcctgaca ttcaatatac ccctaacaag ttaaagattg gggatacgtt tggtttggct
     6481 aaagtaaatg aacaatactt tgcttactca ctgaaataag tttctaatag caagaggaaa
     6541 aaccctcttg ttatttttat cacataatca ttttataaaa acgcttcgaa aatatcaaca
     6601 tttttgaaga aactattttt ctaatttctc aatttcttcc aagggatttt cgctaatgac
     6661 ccatacaggc acagattgct ctcgaggctc cccttcttct aaccaccaac ctgcataatg
     6721 aacttcttcc tcctcagcat taccatgata ccaaccaaca aaggattcat ctacaacgaa
     6781 tgaaatttct tcttctggat caaataattc atttttccat gcttcaattg cttgttcttt
     6841 ggtgtacttt tgtttgttaa atacaccaca aatttcgtca tctacataaa aaatttcaaa
     6901 atcaaaatta ctcgttcctt ttactggcat cttaactttt cctcctttga ctcacttcaa
     6961 tagatatacc actcatgttc attccattgt ttactagtta attggagttt tatcttaggg
     7021 ctttgtgcac tctttccaaa aacttgagcg tcgttttttt cttctaacca aaaaaggtaa
     7081 tttcttacat tcggtactaa atgtttttcc tgcatttctt catgaaaaac atataattct
     7141 gcttcatttt tcaaccaatt cattattcat tccctcattc acttcttcat accgccattc
     7201 agatagtgag atttcttttt gtttctccac ttgtcctata aaaatcgctg tattcaacaa
     7261 gctgatcaca cttaataata aagcaatgaa actaatggtt acacttactc tttcacattt
     7321 tgtcattttg tttccttctt tactcgtttg ttatttcttg taataggtct aataactcct
     7381 tcaccgaaat ttgcaaatgg ttcgcaaaaa tttcaataaa agcactcgca cacattacca
     7441 gaccttcaac tgatatatca ctgcgacaat aaaattctgg agaaacactg atttctgatt
     7501 ctttcttaca attgaacgaa aaaactattt ttgaatcaaa ttccggtaat tcttccacaa
     7561 ctatgttttg ggattctacc aagaaaggga gtgcctgttc taatgcttct tctggtgttt
     7621 gagcagatag aataattgct tcttgttctt ttatttcatt gttctcaaat actttataac
     7681 tttttttcat tgttagttcc tctttcattc ctctctcaat tagctaagcc gttactggta
     7741 cgccattctc taaatttagt atcgctaatc cttaatgctc gtcgaatatc aatcattcga
     7801 taacctattg cacgaagttc tttatatctt ttagcagtta gcgtctctaa cgtcatttca
     7861 ggcaatgggt tcgaatcaaa aatagaattc gaaccgatta tttgggccaa ttgttgtact
     7921 tgtaacagct cttctgaact taattcagtg attgctcgat tgcctatcat tgcatacatt
     7981 ttctgtcgaa tatacttctt ttcttgttta gacaacattt ctatccactc ctattttcat
     8041 tatcatttgt tacctccact tcagtaaatc cacaaataaa tgaattcata ttccaagtac
     8101 cattcccttc aactacaact tttctcctgt cctctttcgg atattcaaga atcagaccgt
     8161 ttaccaatat gtttttcact actactaatt tgtttgtata ccgtggaatt gctgttccga
     8221 tatacttcac tttatttcct acgctaattg tcatttcctc actcctattc ttttttatcc
     8281 aaaacttttt cctgtcctat tcctgccaac gaatatactt cactgcatgc ttaaacaaaa
     8341 caaaatgttg ccctgtttta tttccgtctt ttacttgttc cacatacagt tcatatttgg
     8401 tcacaccttt taaaacgcca gatacttttt ttcatccatt gtcacaatta gttttttcat
     8461 caatcgattg atactgcact gctcattttt cttgttcata tagtattcct ttctttttca
     8521 aaactagcct cttttgttta ctagttttca tcaatcatca acgatttaac agattttttc
     8581 tttcgcaggg tttctagcgt cagcaacccc aaactctaaa aaagtgcctt tgctcccttg
     8641 aaaaacgagc ggacgattgt actcgtcatc atatgttttt catactacct gatccacgca
     8701 aataaccttt ttttgttctg tgccacaccc atccatacgt atcgcttgag attcaccata
     8761 cttttttagg tggtttatcc ttcctaagtc cctgttagac acgctgatta acttagaaag
     8821 tcctgtttgt atccaatttt caaagatctc ccaaaagaaa taagtaggtt tacgtgtatc
     8881 gcttctgttc aaagtacctg tttgtacttg agtatttcta ctcccctcgt tcaagggatt
     8941 ccttccacca gctagctttt cctttcatca tcaaatgttc ctgaaaagca tttacgtttc
     9001 gtgtccgtgt gcttacttat tcttttttcg atagcgatta tccgctcgct actcgctatt
     9061 ttatactttg tttgttagct caaataaaaa ctaacaaaca aaaaaggcta gtttctttcc
     9121 gttttatttt tttaactgtc gataaatagt tgttagttta attccaagtt ctaaaaccaa
     9181 tacgcccaac aaaaccacag gaaaaacaat tggggcaaac aagtaaaatc ggatagtttt
     9241 catgtcttcc ctctttcttt ttggtgtcgc tgtggtcgtt cttgccgttc aatacttcga
     9301 tacatttttt attcattatt tgctcgtttt gttttcctct ggactgcttt tcttgctttt
     9361 tgaatacttt atacgctttt cgtgcctgaa ttaataggtc ttgtcggcgt ttcattcgtg
     9421 agaaaacaat atttgaaacc atcaaatcct ggttcgaaca accacgattc gcaataattt
     9481 tcccatcaaa aaaaatagtt ttcacttgag aaaaaaacag ttggaaatct tgttttgttg
     9541 ctaatttttt tgtcaaaatc actgtgcttt tccctgttga atctacaatg attcttcgaa
     9601 caatccgatt cgataacttc caacgttgcc gattaaacaa tgttacttca caacaaatat
     9661 gacgtaagtg attaggggtc attttcatgt gtccaattcc tccaaccaat tatgcatcga
     9721 aattttgggc aattgtgaca gcccataaat ctgcgtttcc aggtcttcat aactacaaga
     9781 acgcttggct tgaagtaatg aatattctgc ttgatctgat agttttgttg ccattggatc
     9841 tagattcaat tctcttacac aacattccca aaaattcaat agcgctctaa aataaaatcc
     9901 ttttcgattc tgaattgctt ttccttcatg acgagcaatt ttttcatttt gaatacaccg
     9961 ttgatacgtt ttgcttaaat cttctgcaaa tgtttcccct aaaattcgat actcgcttcc
    10021 atcagataaa ccatgtgttt gattatactt tcgttgccgc ttttctataa ttcgtttact
    10081 gtcgtatatc atgtttagct gctctttcgc ttcacgccat tcagctgaac tgcgaacaat
    10141 ccatgtcaaa acatctttcg gaacaaacgt actttcgctg taatgagcaa taaactcttt
    10201 ttgccaatca tcagccattt caacaggact agtcgtatcg atttttgtat ctagattttc
    10261 tgtatctaga ttgttagaag aattattaaa taattgtgca gaattttcac atggattttt
    10321 ttcgtcaaaa tcatcgaaaa tcgctaaatt atcaacagat tcctcctctt cttttccttg
    10381 tgcagaatct acactggttg agtcaacagg acctaatgaa tacacatcat tttctgtcgt
    10441 atctaattct cctagataca accgagcttt gtttcttttt cctgtttgtt gatcaaatcc
    10501 catatcctct atttctagta gctcatattc tttcaattct tttttgattg tattaacaga
    10561 acgtctgcaa caattcaatg cattggctaa ttcttgttcc gtaaattcaa aataaacaaa
    10621 accttgacta tctacttgat ttcgttgaat ggctaaatct aagcgtgacc gaaaaaccat
    10681 atacgcgatt tttgctttat cagacaatga gcgatatttt tcacttgtaa taaatacctt
    10741 tggaaattga tagtataatt cattgtattt acgagttgcg gtaaatcttt ttgttgccat
    10801 cgttattaca cctcatcttg tccatatatt aagaaagaat tttggtgttg gtagacacga
    10861 acatcttcca ttgtagcaat caattctttt agataaattc gaccgaattc aattgttagt
    10921 aagtcgtaat cttctaattc atttttgatt tcttcaattt cttcttcaga cacattgagt
    10981 aaaatggcta attcttgatt ggtataagaa atatagacat gtttcttttc atcaatccgt
    11041 ttttcaatta aggattcttc taagtactca ttcagtaagc tataggcgta ttttgcttgt
    11101 gcagtcatcg cataatattt tgacgatttt ataaagaata gttttggcat atgaaaagtt
    11161 gcttgtgtca gcacatctgt tgcgcgaaat ttgtttttta ccatattttt tcctcctaga
    11221 aaaacacgca aaaaaagctc tgtataaatc gaattacaca gagtttcttc ttgttgatat
    11281 ttaatttcta aaatacgttt cgggtaaaaa ctaaacactt actaatattt agattgtaga
    11341 atcttcgcct tcatttattc attttaaaga gaaaaagatc aagtgaaatg tgcccaaaag
    11401 tttgattcta ttaattttta aacaccattc ataatatatt tcctataatg taaaaagcgt
    11461 cgatacttat taaagcatcg acgttatttt tatagtaaat ttatcaaaat ttttgccaac
    11521 attttttccg atttttttcc ctatttctat ctttccttat ctttcttagt taatattatt
    11581 tgatgttaat atatcactac taatattaaa aaacattgat ttatcatagg ataaagagtg
    11641 atagtttaat acttaaagag acatattaat tttatttaaa aattgacatt aatgactctt
    11701 aatcagcggg tcgcgggttc gagcccctca cggcccattg ggtgccaaac ccacggaaca
    11761 ggtaaatgtt ggtgcgaaag catttcgaaa acatttacgc tgttcttttt ttatttttca
    11821 aaataactag ggctttttat ttttaactca aagtccactg tagccctttg gactttgagt
    11881 taaaaaagaa gcataattat gaacagtaaa gtttctaata taacaataat tcgagaagct
    11941 ttgcaaaatc atgctgccaa tgaactctct aaacatatag gattaaatct aagtactata
    12001 aaaggttaaa aagtgaagaa tatttaatag aaaaactaaa tttacttgct gctttcccct
    12061 tttccattaa tacaacaatc agagagtgtc tttttttcga aaaaattaga ataaataaaa
    12121 gacacgtagt ttttttatct acatgtcttc catcgactac aagttatcat ttctcaaaac
    12181 gtatttagct tttaattgat taatcgtttc acgttgtcta cacattgcct aattaagatt
    12241 ttcttattag aagcatcaat cccgcaataa tacctaaaac tccaggaatt acccacaata
    12301 atgttaaaag aaaaaatgga ataatagata aaatgagtaa actaatacca tagaccactg
    12361 ttttttgatt ttttcttact aaaaaaccta ttatgaaaat cattatcgaa aaaactaggg
    12421 taattcctcc aaataaaaca acattcccgc ttagattgac taacatatct atatccttac
    12481 tggttagttc tgtagtattt aaagtagttg aatcaataga agatagaaac ataccaccca
    12541 tcaatgctaa aagactacat aaaatccctg ttattccact aattaaaatc aaaataaact
    12601 ctgtttttct cttattcatt taattctctc cagattaagt gttttagaat tagaaatttt
    12661 ttgaccatag ttaatagctt ctaatgaacg aataacaacc actgacgtgt tagaaaaata
    12721 atccccaata tgttgtttct tgggcgtgaa agcagccacc aaatacccaa tttgaaatac
    12781 aaaatttttt aatataaacc tacatataca ctcacgaata aagatagttt gccaacttag
    12841 ctctttttcc tcaaagctca ctactcttat cccaaaaatc attttcccaa ttgtttgtcc
    12901 tgaattcagc ttagtcaaaa gtacaaaata ggttaaataa atagctaatg acgctatacc
    12961 ataaaaagta ataatggatt tgtcagattc cagtcccatg attttgtaaa agagacctat
    13021 agtagcatgg gtgatgatgc taatacaaat taaatcaata agaaaagcaa aaaatcgtaa
    13081 ccaaaatccc gcaaatacat aattaggcaa agtttcaatg ccactcttct tattcatttt
    13141 atagccctcc atagtaatac attggttttg gagtatcaga aaatccaagt ttttcaaaaa
    13201 ctgaaattac acgatctgat gtagttgcct ttagcccttg ccattcagca atcttattac
    13261 ctaaccaaga agaagcaaag ccagttgtgt catttttata ttctataacc gtcgcatcag
    13321 ttaatttctt ctcttttttt agggaatcta atgcatcttc agagtaacca atttgatcca
    13381 ccaacccatt ttctactgct tggttaccat cataaattct tccatccgca agttctttca
    13441 ctgcattagt atccatattt cttccttttg ctacaacatt gacaaaacga tcataagcac
    13501 tcatcacaaa ttcctgtata actttattct cttcctctga aggtttgtgt tgaggtgaca
    13561 tcatatcttt taatgcacca cttttatacg tcgcatcagt tacgccaagt ttttctaata
    13621 agccagaata atttaaacca gaaataatga caccaattga tccagttgtt gtttcttccg
    13681 tagcaaatat tttatctgaa ccagcagaaa tgtagtaccc tccacttgcc gctgtatttt
    13741 taaatgcagt atagatagga atatctagct ttttaatttt tgccatttct ttagcaatct
    13801 cagcactttc atatattcct cccccaggtg aatttacttc taataacact cccttaaccg
    13861 ctttgtcttc ttgaattttc tttagctgtg ttaaaaaatt ctgatggtta tattgttccc
    13921 tactaaaaag attgctttca cctgtatctg caataactcc attgacggat agtttgacaa
    13981 tctttttatt tgatgcgcct tcttctaacg tttcttctgt gatttcgtta gatccgtaaa
    14041 aaatcttatt tattcctgtt agggttgtat ttgatgcttc ctctttatct tttcctggaa
    14101 ttagcgcgac gataatggat agtaacaata atccgcacgc tactcccaca gcaatccatc
    14161 ttcttttatt catataaact cctcctatat tcttagtaaa aataattatt ttttatttag
    14221 aaacacatcg acaatttgtt gccaatatgt tttttctcct acaattaaag acaactctcc
    14281 agttaatcca taacgaaacg gaaacgattg agttgttttt aacgttcctt tcactgcata
    14341 aaaaacacct ttctcagtgg cagtagaatt ttctgaaatt tcatctaaag ttccgtcgat
    14401 cgtaatcgga gccccacctt tttgatccaa tttaaaatga accttcatcc cttctttaat
    14461 ataggtactt tcattcgcag gtaaaagtgc tgtaaatttc atttttgatt tttcgtggat
    14521 cggataaatt tctgcaagca ctgttccttt ggctagttct gcttgtccct ttgtttcttc
    14581 atttaaatga accgttccag atacaggagc ttttagtatt ccttttgaaa gttgatcgtc
    14641 caaacttttg ataactgact ggttcttctc ttgtttttca tttagctctg taactgtttg
    14701 ttttacagtt gttattgctt gttctagctc ttgctttcct ttttccgttt gactaattct
    14761 gtcattctca gaagtagttg gtttaactaa tttagctttt tctgactgat actgttctac
    14821 ttcttttttt agttgctcaa tttgttcatt tatgcttgct gaaattgtca gtttcacttg
    14881 atttttttga tcatcagaaa cattatttag ttgttcttgc caattctcat agtttgccat
    14941 catttctttt gaaaaatctt ttaatgattg attattactc caagctacct gtacttgttg
    15001 ccattcttgt aatttactat ctgaactctc aatttgtttt tctagttgtc tttttgtctt
    15061 ttcgtatact tctaattgct tttgatcatt taattcactt tgcttcaaag cgtagcggag
    15121 tgattctttt tctgacaaca tgctctttaa ttgattactg tagccaaaac tgtcatccgt
    15181 tgaaaacaaa ttttttcctt tacttatact ttcaataaat agctgagcca tctttatttg
    15241 ttgagtaata cgttggtttt cttgttctat ctgagccttt tcattctcta ttaaagaaca
    15301 atccaatcta accaacacat cgtccttatg aacaaattga ttttctttta ggaagttttc
    15361 cattatttta gcttctaatg gcacttgtaa cttctctatt ttttcggggt tcaattgagc
    15421 cgttgtccga ataacaacct cttttttagc aaaggttaaa aacaatccaa taatacataa
    15481 aaaaagaatt acagggtata acacccaacg gtagaagcta tgatgttgtt tagtatagat
    15541 actagaatgt tcgaaccaat tattattctg cataaattgt ttccctctcc tagatttccc
    15601 ataatttttg atagatacca tcggcaaaac gaagttcatc gtgcgttcct ttttctacca
    15661 aatgcccttc atggagaact aaaatttgat cacatgcttt tgctattggt aaatgatgcg
    15721 caataaaaat aatcgttttt tcttttaaat tgaggagata attcataatt gcatgttcta
    15781 acaaggtatc taatccacta gttgcttcat ctaaaattaa tatgtccgga ttttttaata
    15841 aagcccgtgc taaggctaat cgttgtcgtt gccctcctga aagattattt ccaccttctt
    15901 ccaacaatgt ctcgtaacgt aatggctgtt gattaataaa ttcatttagt tggactatct
    15961 cacaaacttc tattatttgt tcgaaacttg gtgggctatc taaaccgaat ataagattct
    16021 caatgattgt tccactaaag aaaaatgact cttgaggcaa atatgtcact cgatcgcgta
    16081 atatctgaaa aggaatgtct aagtgattaa tgtttccata atggatcgat ccttctgatg
    16141 cttcataaaa acgaacaaat aactttgcaa gagttgattt tccagaacca ctaactccaa
    16201 ctatagccgt tttactgtgc gaaggaattg tacatgaaat ttcgttcaac gttggggcct
    16261 tcatgctata cgagaatgaa acgtttttta cagtgatatc ttgttgaaat aattgttcag
    16321 aaagcatata ttcaaaagac tggttcgatt tttctgaatc aatcgcgaaa atttcattta
    16381 aacgattatt tgcaacttga gccgtttgca ttttcacttg taaattaata atgttttgta
    16441 aaggatctgt caaaaatact agtaaagcat tgtatgtgat aagttgacct agactaattc
    16501 tattatccat aacaaaataa gaacctagcc ataaaattaa tgcactgcta attaattgaa
    16561 tcaaatgttt aattccttgt tgaatattat caagcgtaac cgtacgaaat gattttttca
    16621 ttaatttaac aaattcatgg tctatacggt tatatacttt ctcttcaccg ctatatgctt
    16681 taattgtttc tattccttta agactttcta tgatactaga atttacagct gcaccggctg
    16741 ccatttcttc cctgttcgat ttttcatagc ttttcacgaa agcgaatatt gcaactacat
    16801 aaaaaggcaa agagcataga gtgattaaaa acaacgtact attctgaata gctaacgtaa
    16861 ccccaacaag caacaccata ccaatatcta aaaatattga taacgtagca cttgctaaag
    16921 catcaataat cttactggca tcaagaaaac gagaaataat ttcccctgat tttctcgtag
    16981 caaaaaaatt cattggtaaa gttaacacat gtttgaaata ctgaagcata attgccatac
    17041 tcattcgctg tcctaaaata ataagaagat aactacgact atattcaaac aatacccgaa
    17101 ataaatagac agcgatcaag ccaatggaaa ttatatttaa agttgaacgt gcttgattag
    17161 ggatgaaaaa atcaagaata ccttgaaaat aatatgagct tcctatgcca aaaaacgtaa
    17221 tgaaaatcga tgctaaaacg atgtggaata ctaatccttt ttgtttccaa attataggca
    17281 aaaaagaact taatccagct actttttctt taataggaat ataagtttct ctaggcatag
    17341 gtaataaaag aatacctgtc cattcttttt gaaacgcttc aatcgttttt ctattttttc
    17401 cattagcggg atctgcaatc catagtacgt ttcccttcgt tttataaatt acaacgtagt
    17461 gcatatagct gttatcaacc aatatatgtg caattaatgg taagataaga tctctttctt
    17521 tccagatctc actatctgct tgaatagcca tacaatcaaa agacaatttt tcaaatgtct
    17581 ttttcaatcc aaatgctgaa gtaccctcta aatcagtccc agataggtcc cgtaatttat
    17641 gaatcgggat atcagtttta aaatatttta aaatcatcga aaggcaagca acaccacaat
    17701 ctttttcatc ttgttgctgc acaaaaggaa atgattttat cacttatcta acacaactcc
    17761 ttctaaaaaa tttttataga aaaattctca atatagcttt aatacagtga aaatattgtc
    17821 tcataattcc acaaaaactt tgaaaacagt tcttgaacac taactttttc attcctgaat
    17881 caaatttatt ttggaaattt tactattcaa gaataaactt gataagtttc aggaagattt
    17941 ttactgttaa tcaaatacgt atgttacagt agcaccgtaa aaaatattat ttaaagaagc
    18001 gtgtttaaac atgcaaaatg taaaggaact aagtattgtg gaaatgcaca aactgttggt
    18061 ggaggcaata atgcttgggg aaaattgggg caagttgtag gtggacttac cacaggggca
    18121 gtaggtggtg caggtttagg aacagcaatt tgcggaccag cttgcggtgt tgttggaggg
    18181 ttatatggag ctgtcgctgg aggtgctgca gctggctggg atgctcgaaa aaaataaaaa
    18241 aggagaataa gtagtatgca tattaaaaac acaaaaacaa catttatatt atcttcagaa
    18301 gagttgaaaa atattcaagg tgggagtgcc gtgggagttt taggcactac tttttctggg
    18361 gcaacagcag gagtaaaact ttgttcagcc ggaggacctt atgcaatagc tgcttgtggt
    18421 gtaggtggag ctttattagg tgctggattc agcatgtgga ccggtgcgta aacaattaaa
    18481 aaatatcaaa cctcaagatt ggtttgtgtc gattacgaac ataggtatgt tctttatctt
    18541 ttcaaaagtt tggaatggac agaacttttt actaaagctt cttgtgctag tttttataat
    18601 tatattaaat ttttatcttt tattaaagtc agataaaaat tctttggaaa tgtaatggta
    18661 gagatatttt tacaaaagga cttgcagcgc tgtagttggt gctgcaggag gacctatttc
    18721 ggccgttaca ggaggtattg gtaatgtact tgtcggatgt gcattaaagc ctgttcctgc
    18781 aagttaaatt tataaaaaca ggaaagtaat tatctcaaat cctatcaagt acaatcatag
    18841 gattgcactt gacaggacaa attttttaac taaaaaatga aaaacacttt ctaaaaatta
    18901 gtagaatttg ccaaatcttt cgcatcttcc accatacatt taattttaat tcccctttat
    18961 ggtacttaat aaaaacaaga aaagaactat tcatttggcc cttttttggt tccagaccat
    19021 agaaatattc ctccagtaac taatattgct gttatgcacg taactaataa cagctataaa
    19081 aattggtaca actttaatag gattcagaaa aatttgcact agtgaaggaa tctattttat
    19141 tagaaaaatg caatctccct aaagaaaaaa ccactaaaca gtgctttctt tcaacttctc
    19201 cttaattttt tgtgctttcc ctaaagtaaa gtttctggcc aactctgaat caaaatatag
    19261 tgaacgtttt ttaaaatcta tttctttaac gttaaagata tttactaaac acgctcgact
    19321 aattcttgtt aacataggat atttcttttc aagctcacta attcttccat aaaattcgta
    19381 ctgcccatct ttagtacaca atgataatct atgtggtaat tttgaagctt ctacaaaata
    19441 aatgtcattt atatcaaaag taaatgtttg agaacctatg gaaaaaacaa atgcttgatt
    19501 cgtaacctgt tttgttgcat ctatcctttc ttgcgctaat aacaataact caacaatctc
    19561 gtttcgatac tcatctagtg tttgatcttt tgttacaaat cctaacgttt caactcttct
    19621 ttttattgtt acgggtaaca tctcatcatg agtagttgta aaaatgattt ttgcttgaac
    19681 atcatatttt ctgattactt ctgctaattc tataccgtta acttcatgat ttaaatcaat
    19741 atctaagaaa tatattcctt gctttggtcg gaattgtttg aggtattttt taacttctaa
    19801 cggactttgt gttttcaata ctatcttaaa tactttatcg tgaaaaagaa tgaaattatc
    19861 aataattcgt tctaactgat ttaattgtat aatttgatct tcacataata ttattggata
    19921 actcataaat cctccttccc tcaaaacaac aaaataactt gagtagtaaa ttgatacgga
    19981 tcgttttgat agttcacaaa catattggga ttctttttat tgatttcctc tatactagat
    20041 aaacctaacc cttgatgtcc cttttttgtt gttattcctt ttttcattag agtatctata
    20101 gagagtagta tctctctaca actattcgat atcgaaattt ctatctgttt atcatctcgg
    20161 taaatagcta gagacagttc tctatcttga ctctctatag atgcttcaat tgcattatcg
    20221 agaataattc caagcaccct aatacagtca aatattggaa caggaacttc aataatagta
    20281 cttggacat
//
LOCUS       DACDTO010000049        19821 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDTO010000049 DACDTO010000000
VERSION     DACDTO010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.049"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWK70_001759"
     CDS             6..305
                     /locus_tag="IWK70_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9614287.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWK70_001760"
     CDS             378..1739
                     /locus_tag="IWK70_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9614288.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWK70_001761"
     CDS             1761..2021
                     /locus_tag="IWK70_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614289.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWK70_001762"
     CDS             2035..2787
                     /locus_tag="IWK70_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9614290.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWK70_001763"
     CDS             complement(2881..3486)
                     /locus_tag="IWK70_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9614291.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWK70_001764"
     CDS             complement(3539..4513)
                     /locus_tag="IWK70_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9614292.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWK70_001765"
     CDS             complement(4891..5670)
                     /locus_tag="IWK70_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9614293.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWK70_001766"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWK70_001766"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9614294.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWK70_001767"
     CDS             7161..8558
                     /locus_tag="IWK70_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9614295.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWK70_001768"
     CDS             8639..9079
                     /locus_tag="IWK70_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9614296.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWK70_001769"
     CDS             9227..9553
                     /locus_tag="IWK70_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9614297.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWK70_001770"
     CDS             9554..10303
                     /locus_tag="IWK70_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614298.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWK70_001771"
     CDS             complement(10383..10838)
                     /locus_tag="IWK70_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9614299.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWK70_001772"
     CDS             complement(11265..11540)
                     /locus_tag="IWK70_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614300.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWK70_001773"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWK70_001773"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9614301.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWK70_001774"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="IWK70_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9614302.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="IWK70_001775"
     CDS             14770..17436
                     /locus_tag="IWK70_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9614303.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="IWK70_001776"
     CDS             17800..18552
                     /locus_tag="IWK70_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9614304.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="IWK70_001777"
     CDS             complement(18574..19728)
                     /locus_tag="IWK70_001777"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9614305.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
BASE COUNT         6330 a         3473 c         4060 g         5958 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagccg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat t
//
LOCUS       DACDTO010000050        19475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDTO010000050 DACDTO010000000
VERSION     DACDTO010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.050"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(21..995)
                     /locus_tag="IWK70_001778"
     CDS             complement(21..995)
                     /locus_tag="IWK70_001778"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9614306.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1075..1950)
                     /locus_tag="IWK70_001779"
     CDS             complement(1075..1950)
                     /locus_tag="IWK70_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9614307.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2080..3987
                     /locus_tag="IWK70_001780"
     CDS             2080..3987
                     /locus_tag="IWK70_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9614308.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4528..7806)
                     /locus_tag="IWK70_001781"
     CDS             complement(4528..7806)
                     /locus_tag="IWK70_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9614309.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(8077..8622)
                     /locus_tag="IWK70_001782"
     CDS             complement(8077..8622)
                     /locus_tag="IWK70_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9614310.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(8634..9326)
                     /locus_tag="IWK70_001783"
     CDS             complement(8634..9326)
                     /locus_tag="IWK70_001783"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9614311.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(9345..9629)
                     /locus_tag="IWK70_001784"
     CDS             complement(9345..9629)
                     /locus_tag="IWK70_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614312.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(9642..10205)
                     /locus_tag="IWK70_001785"
     CDS             complement(9642..10205)
                     /locus_tag="IWK70_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9614313.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            10433..11095
                     /locus_tag="IWK70_001786"
     CDS             10433..11095
                     /locus_tag="IWK70_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9614314.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            11219..12415
                     /locus_tag="IWK70_001787"
     CDS             11219..12415
                     /locus_tag="IWK70_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9614315.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(12516..13892)
                     /locus_tag="IWK70_001788"
     CDS             complement(12516..13892)
                     /locus_tag="IWK70_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9614316.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(13929..14795)
                     /locus_tag="IWK70_001789"
                     /pseudo
     CDS             complement(13929..14795)
                     /locus_tag="IWK70_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
     gene            complement(14884..15909)
                     /locus_tag="IWK70_001790"
     CDS             complement(14884..15909)
                     /locus_tag="IWK70_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9614317.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(15927..16601)
                     /locus_tag="IWK70_001791"
     CDS             complement(15927..16601)
                     /locus_tag="IWK70_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614318.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(16785..17678)
                     /locus_tag="IWK70_001792"
     CDS             complement(16785..17678)
                     /locus_tag="IWK70_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9614319.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(17752..19449)
                     /locus_tag="IWK70_001793"
     CDS             complement(17752..19449)
                     /locus_tag="IWK70_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9614320.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         5684 a         4190 c         3444 g         6157 t
ORIGIN      
        1 tctttccctc ttttttttgt ttattcgaat tctttttctt ccagacggaa taacggactc
       61 atcggtgtat tttcatggat gcgtttgacc gcttcaccca ttaattctga acatgtaacg
      121 attgttaagt tttctggatg acgattttct tctgtgaaaa cagaatcagt gatacaaata
      181 tctttgattg gtgcattgtc taaaatactt ttcgcatgat cggacaataa tccatgtgac
      241 gcacaggcat atacttcttt tgcgccgttt tcttttaata catttgctgc tcgtgcaagc
      301 gttgctcctg tgttcaagat atcatctacc atgatacatg tctttccttg aacatcaccg
      361 atcacataac caccctcagt tgaagcatca tctgcatgat ctacgatggc taatgtgctg
      421 ttcaaatatt ctgaaagact acgtgcacgc tgaacaccgc tgttttttgg cgaaacaact
      481 acgatgtcat ctcccgtcag acctaattga cggtaataat gagcaaacaa cggcatcgta
      541 aataaattat ctacaggaat atcaaagaat ccttgtactt gaaccgtgtg cagatctaaa
      601 gtcagtactc gagttgcccc tgcttcttcg atcaagttag cgatcaattt cgcagtgatc
      661 ggttcatgag gtttcgccgt gcggtcttgc ctagcatacc catagtaagg caatacgaca
      721 ttgatcgttt tcgcacttgc tcgcttcatt gcatcgatca tgatcagtag ttccatatag
      781 tagtcattga caggtgcatt cgttgattga acgatataca catgatcccc tcgaatactt
      841 tcttcgatat tgatggaaat ctcgccatca ctaaattgct tcacgacgct tttacctaat
      901 tctgttccga acactttcgc gattttttca gctaatggac ggttgccatt caaactaaaa
      961 atcttcaacg tgtcatcctg atacttatta ctcatgtagt cctccccaaa acttctctat
     1021 tcttcgatag tttaacatat ttttccaaga ttttctgtta atttcagcaa attcttaagg
     1081 aagtgaaact gttccgttac tccaactggc aggaatgact ttatgataat cgccccagtt
     1141 gtcgtgtact ttgtaataac gttctcctgt ttcagtttca aaataagcgt acgctgtcac
     1201 ccaatgattg ccatatgtac tgccaagtac tttcaaaata ccaatcatca caggtttgcc
     1261 ttcacgtata cgttttgtag ctcgctgcca tgaacctaga agagttgctc gtgcacgctc
     1321 atgattgcca ttcttcttta aaaaactaga aatccctgta ctaacctgaa aaggaatcgt
     1381 aggaagtcct agtggttgga tctggctacg caatgccttc gtcaaaacca acgaatcagc
     1441 cgtatttttc ttgcgaatct catttggaag catccacccc ttacgatagt cttgataata
     1501 ggcaaggaga actgctgccg cgtaagtacc acaaagatag cctgaggggg tagcccattt
     1561 cttatacctc aaaaaacgtt cttctggcaa tcccacccag caatttgcga gttcctttcc
     1621 tgaccttttt tcttcgatca aaacgggaat atcttctttt ctgttgatcc atctagataa
     1681 ctcgattggc tcttcattga tcagcttcca ttttttacaa gaaagaccct cttgataagt
     1741 atattttgct ggagaatatt ctaccggatg tcctgattga tcgataatca cataactttg
     1801 atcttcaaaa accagagcta aaaaaagcaa tttacttttt cttgactgca gctgctgagt
     1861 gctggctaat tttttaattt ttttatctac tttgaaatat aaaggtttat ccactacttg
     1921 attcagttgg gatcgcctcc tttttttcat tataagtgaa ggattgaaaa aaacaaatca
     1981 gtttgattgt tcgaattttc aattttttat gttctatttg ttaattcctt cattttgtaa
     2041 gataatgggc acacgaaaca aaaaaggagc gatcgatgaa tgaacaaaga acgattgaaa
     2101 caagatttct atgaagcagt caatgaagaa tggcaaaaaa cagcaaaaat ccctgcagat
     2161 aaaccagcta caggcggttt ccaagattta gttgacggga tcgaccaact tttgatggga
     2221 gaaacagacg atctgcttgc ccatccagaa aaagtgtctt cagaaaaaat gaaacatttc
     2281 cttgcatatt atcaactggc aaacgattat gaccgaagaa atgagttgca tgctcaaccg
     2341 cttcaaccta taatcgaaaa agttgaatca ttgcagtctt tcgacgaatt gaataagcaa
     2401 tttccagaat ggctgttgga tacattacca ttgccattta atctggatgt agatgcagat
     2461 atgaagaatg cgcaaacgaa cgccttcttt gcttatccgc cttctttatt ccttccagat
     2521 aagacgtatt atgcatcaga ccatcccaac ggtgcacaac taataggcgt tttctttgat
     2581 atggctgttc agttattaga gcttgccggc aaagaaaaaa gtcaggcaga agcaatcacg
     2641 gaacaagcag tgcaatttga taaattgatt gctcctcatg taaaaagtgc agaagaaaac
     2701 gcagattaca gcaaaatgta taatccgcaa tcttttgata catttattaa atacacagat
     2761 aaactggact tgaaaaaact tgtcacgggt ttgattggga caactccaga taaagtgatc
     2821 gtaactgatc cagtgtactt cgatcatttg aatgacatcc tgacagaagc tcattttccg
     2881 ttattcaaaa gctggatgat cattaaaaca gtacgttctc ttagtggtta tctatcagaa
     2941 gaattccgcc aagtcagcgg aatctattct cgcgtattgt ctggaacaga cgaagcaatg
     3001 cctcagaaaa aagcagcgta ttaccttgcc tctggacaat tcgatcaagt agttggcgat
     3061 tactacggaa aaaaatattt tggcgaagct gctaaaaaag acgtagaaca aatggtgaaa
     3121 aaaatgatcg ccgtttataa aaaacgtcta gaagaaaatg actggcttgg ggatgcaaca
     3181 aaagaaaaag ccgtcataaa attaaataac ttaggtatcc aagtaggcta ccccgataag
     3241 atcccagcat tattcgacca gttcaaaaca gtacctgcag atcagggtgg tactttgctt
     3301 tcaaatactc ttgttttcag caaactcaca ttaaaagatc gtttcagcaa atggaacaaa
     3361 ccagtggatc gttcagaatg ggaaatgagt gctgacacgg tcaatgctta ttatcatcca
     3421 tttcggaatg tgatcgtctt tccggcagcg atccttcaag caccatttta cagtctagaa
     3481 caatcaagct ctgctaatta tggtggaatc ggagctgtta tcgctcatga gatttcccat
     3541 gcatttgata ataacggcgc tttatttgac gaatatggca acttgaacaa ctggtggaca
     3601 gaagaagatc ttacacaatt tcaaaacaaa gcacaagcaa tgatcgaaga attcgatggt
     3661 ctgccttttg ctgacggaaa agtaaacgga aaattaaccg tttcggaaaa tattgctgat
     3721 gcaggcggtc taagctgtgc attggaagca gcaaaaacgg aatcagatct ttctatcgag
     3781 gaattctttg tcaattgggc aacgatttgg cgtacaaaag caaaaaaaga ataccaacaa
     3841 cttttactgc aaatcgatgt ccatgcccct gctaaactcc gagcaaacgt gcaaccaaaa
     3901 aaccttgacg aattctatga aacatttggt atcacggaac aagacgctat gtatctagcg
     3961 ccagaaaaaa gagtgcgaat ctggtaagag gttgtgccag aagtggtcag cttcaagaaa
     4021 taagaaggaa tttgggaaaa tcgcttctca aatttttgtg acatttcggc ttatttttcg
     4081 aagaagttac ttctggaata ccatgtatta gaggttgtga aaaagctgct tagtttcaag
     4141 tagtaagaaa aattttgtga aaatggcttt ccacattttt gcataaattt gacttactac
     4201 cgagaaactg gcttttgaac accgtttatc gagaggttgt gaaatcagcg tcctgacctg
     4261 agtattagag aaaaaaacgg aaaaatagtt cctcatattt tgccgtttgt tgagctaata
     4321 ccgagggtca gctgattgaa caccgtttag tggagagatt gtgaaaaagc tgtcctgcat
     4381 caagtaataa gaacagccaa tcaaaagcag caaggagaac ctcatctata aaaagataga
     4441 aggaagtgag acatctattg gtctcatttc cttctatttt tactttcctc agtcaattgt
     4501 tttacatcat aaaaggagaa attccactta tttttttcgc tgtttcatga cataaacaac
     4561 tacaccgatt aacaaaattc ctccagctaa tgctagcaac gggttattaa attctcccgt
     4621 ttttgggagt ccttgagtgc catttttttc actttctttt gctgtcctat ccggatgaga
     4681 aggttttcca tttggttttc catttggtga ttcatttcct ggattttctc tttctggttt
     4741 gtcatttccc gggggcacat cggcagtttc tcgcacagtg atcgcttttt gctgggtaac
     4801 agattctgta tgagcagacc atttttcccc atcaaatatt tccgaacgat aagtgacttc
     4861 tagcgtatat tctccttttg aaggtgctga aaaaatagcg gtatgattag aaaattgagc
     4921 acttgactgt gtttcaacga tttggatctt ttctgggaaa taacgcatat cttcaaagac
     4981 aggcgttcct tgatttggtg tgaaagttga cacaaaaact gtaaaaggct tcagttcaat
     5041 gatatttcct tctgcattct ccgccacacc atttgcttct tgtataataa tcggattatt
     5101 ttgttcattt ggttttgtct gatttggttc ttgtacgatt gcatactctg gatattccga
     5161 agacggtgag gtttctccgt caaaaatagc acctacagat aatggaagac catccactct
     5221 ttccttttct gtttgacgat tccccggttt aatctgcatc agtccatgaa catggatact
     5281 tccattttca tagcgcgtaa caggctgctg accttctttc gaacatgtat ttagagactc
     5341 gatccaatct tttgatatgg tatgtccaag agtattttct gattgtttct ctcgttcatt
     5401 ccaatagtag ttcaacgagt ggtagatttg atcttcgttt tgattcaatg ggcggatctg
     5461 ctctctcatc tcaagattct ctgttcggaa agataagata ccagaagcat ggtagtctgt
     5521 gtttcctgct gaagaggtat aaagagccac attatatgaa ccgttattaa aacttgtact
     5581 gctattgacg ataatatctg ttccgctatt ggaatcgatt cctttcgcaa gattttcgaa
     5641 agacaaacta ttgatcaatt catgagggcc cgttaaagaa gaaccgccca ttttgaatcc
     5701 attaccgtta ccttctccct caattcctgg tacccagccg tttcgataag caactgagtt
     5761 tttgatgatt acttttccga tcgaacctgt ttcattttta gcaaaaaggt cccaaccgtc
     5821 atctgcatta tgatacgcga tacaaccgtc aaatacattt ccttctccta ttgtcagttt
     5881 ggccgcaaat ccatcggcat cttcaaatcc tggatcggca ttttcaaaag atgtacagtt
     5941 cttgactaag ttatgcgcgg gccatgcttc gaaaggatca tttgagctac cgctgatttg
     6001 gattcctgta tttccattcc gataagcatg aatctcttcg atcaaattat gatttccaga
     6061 aatctgtaat cctttttgca tagctgcact atttgtaatg tcaaaccctc gtaaacccca
     6121 gtagtgactg aacagtgtca tcccatttcc agttccttta aaatcaagta caggacgctt
     6181 gttttctgga tttttctctg caatcaactg aatcgggttt tctcttgtcc cgttaacgcc
     6241 ttttggaatc gtcaatcctc tttcgaagga ataagttcca ggcgccatga cgattgtctg
     6301 cccagcatat gcgtatctca atgcttgtgt cagagataat ggattttgtc tgcttccgtt
     6361 tcctgcatct gacccttctg gtgttacata aactgtcgaa tatttaggtt tacggtagtc
     6421 cactgtatga agaatctctt ttgtttcata actagtcatc tctacgtatt cttctggcgg
     6481 atagttttta tctggcgtaa aactgaaagt aaattcgttc gttccttcca tcagttttgt
     6541 ctgataatca aattcccgat tcgcagtgat agccaagttt tttgcgttct taagcacctc
     6601 tcctgttgct ttatttttaa ttgtcaataa tccgtcagca tttgctctaa aggacatagt
     6661 atgatcttgg tttcctgaat gattactaga ggtcacagtg taaatcggat caatcaagcg
     6721 agtaggtctt tcttgcccag gaggatctgt cgcaggatct gagtaagcaa cttcgattcc
     6781 ttctaccttg atcgtcatat tccttgctgc agcaaatcct gcataaataa ccgattcatc
     6841 tgaagcgtat aatttttccc agtcatacat gatgtcagaa gccatttctt ctcctgtttc
     6901 aggatcgtaa taatatgact ctaaaccagt attcgttttt ttcatctcta cgtccagttt
     6961 agtaatcgag ttcgtggccg gaaacaaatt cccatgaccg tttccgaata tattataaga
     7021 acctgtttct ttttccaaga accctgctga agtctctaaa ggtgttgttt ccactgagac
     7081 actcccagga gctggcgcag cttgcggatc ttcagatgat attcctgtga ttgcccgtgt
     7141 ccccaatcca agtcgcatcg tatatttgct gccgctcgtg ttgaccactt cttgtctatc
     7201 cgagtcccag acatattcga tccgactgcc tagtatggca aatgaatttg aataaaatgc
     7261 ctgcccaaac aaatgatttt ttggtactgc gtctcttacc attaaagcaa atccttcttg
     7321 ggcattcgta taggtccaag attctatttc gatcgtcgcc cgcatcctaa agttgatgtc
     7381 tttggggatt gcttgataat aataggtcat tccatctgat ccgcttgact ggaattttcc
     7441 gccgttattc gtggaagaga gccgcacgcc gtcttctaat ggttcaatgg catacctact
     7501 agcacttgtg ctttctccga aaacaacccc tttccactct tcatttgctt cttcagattg
     7561 cttttttgtc ttttcatttt ctgattcttt catcatagga tcatttcctt cttccgtatc
     7621 catatcttca ctagttcttg gtacttcctc cattgttgac atcccttcaa tgttcacgtg
     7681 tgcattttct tcgttcacag actgttgttc gttagcagca acttcaacga cagaaattaa
     7741 aaccggggta ctgagcataa ggagattcat cattagcgca ctgtatttta tacgtttttt
     7801 taacaagtgt attcctcccg taacaaatta gaaagcgttt acttaaagta ttacctaatt
     7861 tatttttact gtcaataaaa aacaaaatta taataagtgt attttataaa aagaaaaaat
     7921 ataacttaag ttgttgataa taaagagatt tattaacgta aaaagaataa gtttttttaa
     7981 aataacgtat aaaaaagagc ataaaacaaa gtcgtttgtt ttatgctctt cttttattat
     8041 tttgtgcttt ttattttatc aaatcacttt gtttttttat aaaatttctg cgcctagtgt
     8101 ggactcaaaa tgtctcaatg cccattcatg tccaacggga tcgaaacttg ccacggcatc
     8161 tttgaaaata tggagtttgt atcctagatt atacgcatcg actgctgtat gcaacacaca
     8221 gatatctgta catacgccgg tcaaataaat atctgtgatc tggcgttctc gcaaacggat
     8281 gtctagatct gttccactaa aagcggaata atgccgtttg tctatccaat aaacattttc
     8341 ctcttgctca tagtcttggt ataaactttt taaagagccg tacaattccc gtccacccgt
     8401 tcctatcaca ttatgcggag gaaataagcg gttctccgga tgaaaggaat cttttggatc
     8461 atgcgcatca atggcaaaaa cgacaaaatc cttttgatca ataaacttct ttgtatgagc
     8521 agtcagcttt ttttcaatta cttgtcctgc tgtcccagta gtcagctttc catcctctgc
     8581 aacaaaatca tatgtgtagt caatcgagat caatgcgttc atcttagtcc tccttatacg
     8641 agatgtttga caaattcgat caccatttct gctgatcgtt tgcctgcgtc ttcgataaat
     8701 tcgtcaaagc tttgtgtcgc cgagtgatct gctgtgtcgc tcattgcacg tacgatcaaa
     8761 aatgggatat tgaactgacg agctgtttgt ccaacagcgg ctccttccat ttcgcaagct
     8821 aatgcttcag ggaaattcgt taagatttct ttgattttat ctggcgagtc gacaaatgta
     8881 tcacctgtaa cgatcaatcc ttcttttgcg ttcaaattcg ttgcttttgc tgctttaacc
     8941 atttcactgc gcaaatatgt gcttgcttca taatatagag gcattcctgg taattgtcct
     9001 ggtttgtagc caaatcctgt tgcatctgca tcaaaataag ctactttgtc tgatataacg
     9061 atgtcaccta cttgtaagcc ttcaccgatt ccgccagctg aacctgtatt gatcaccatg
     9121 ttcacgccgt attgttggat caacagactt gtggtaatag atgctaagac ttttcctata
     9181 ccggaacgta cgacgatcac ttcgtggtta cctaatgaac ctgaaataaa taaagcgcct
     9241 gcgcgttccc atgataatgg ttcgcttagg ttctctctca aaatttttac ttcttcttcc
     9301 attgcgccga taataccgat tttcatcttt ttaattcctt ctttctatat aaatgcgatt
     9361 gctaaaaaaa ccacacataa cattaggata acaataataa gagatttcat caaaaaagag
     9421 ttccatttgg tcgtcttttc ccgttcgctg atccttgttt tcgtgattgg tttattcttt
     9481 ttgctttctt tacgataaaa gctggcaatc tttttttctt cttgctgata agctgtttct
     9541 gcttttcgct gtttttttaa tgactcgctt gcttgtgctt gttgtcgctt gcgaagttcg
     9601 cttcgcgtaa taagcggtcc tttggccata agccgatccc tctactttcc tttggtcaat
     9661 agttcccagt attgaatcgc ataaatcgtc tttgcatcac aaatcaactg atctttcatc
     9721 gcttgttgcg cctcttcaag agttagatga tacaactcca gtacttcgtc ttcatcttgt
     9781 gctaaaggat tctcgacctt ctctacccct tgtgcatgat aaatatgaag gacttcattt
     9841 gcaaaacctg gtgaaagata catcgaagta agatggctca gcgacttcgc acgatagcca
     9901 gtttcttctt ctaattctct agctgctgtc atttcgggat tttgtccttc tcctggatcg
     9961 atttttccag ctggaatctc caaaatcact ttttctaaag gttttcgaaa ttgtttgaca
    10021 agaagcagac gatcttgttc gtcaaaagca atgatcccga caccaccagg atgaaatacc
    10081 agttctctct tcgaagttcc tccatctggc aaacgaactt cgtctacagc aacatcaata
    10141 atttttccgc tatagatttc tttgcgactg attgtttttt cttcaaattg ctcattgttc
    10201 agcatatctt ctcctgcttt ctttgtaatc ataactgctt ttttttcatt tttcaatttt
    10261 tctcaaaaac tccttattag tttaccatag ataaacctta gattttctct aaatatagaa
    10321 aatccttctt tagtccgata ctctttctag tagtccgtat gctaaaataa gtatgtaatc
    10381 gaataacgaa aagaattcaa tactcaacga taaatgacat ggagggttta ttatgataaa
    10441 acgaaattgg ccgtggcttc tagcattggt cttgctattt ctctttgtcc gaaacaatag
    10501 tttttctttt gttctaggac ttttattgat aggtgtagtt ttagttttgt ggggactctt
    10561 tggttcgcgc aggaaaaagg gaaaacaaga agaaattcct gcactgactg acgaacttga
    10621 aactcactat gaaaaaagcg gtatgacagc gaatgaaatt tcattttttc gacaaacaat
    10681 gaatcaaaca aaaaatgaga tcaatcaatt gcaaatgaat atgcagcaga cagccaaact
    10741 taaagcaatc gatctccgcc atgatccagt caaagcagcg aaagctttat tcaaagaatt
    10801 ggtgaaagaa ccaaaccgac tgcatgaagc aagtcagttt ctttatacgc atctgccgaa
    10861 tctagttgat ttgacaaata agtataatga gatcaatgac catgagatca aaaacaaaca
    10921 gacctatgaa aaactagaag aaagcgcgca gatcatcgac cagctgtcag ctttaatcgc
    10981 acaagattat caaaaattcg ttgctgagga tctagatgat atcgacgtag agatatctgt
    11041 tgcgaaacag agcttaaagc gtgataataa atatgaacca gaacaaaagg attaactctt
    11101 ttgttcataa aagaagactc ggaacattca gatgatgtct ggctgtttcg agtacttttt
    11161 ctaaagtaga gacattcatg taagaatcga gaacagattc aacaaggagg aattttttat
    11221 ggatcataaa ccgactgaaa caaaagatgt tacaccagtt aacgatacac ttgaagattt
    11281 attgaacaac ccgttttcaa ctccaacaga tacgctgacc acttcacaac aagcggagat
    11341 tgatcagctc caagagcaac agacagctgc tcgtttaatt gacaaactac cggcggaacg
    11401 ccaagaacaa gctaaacagc tagcagcaaa aatcgatgct aatgatgcac agtctgtcat
    11461 cagctacgga tcggctgccc aagcaaaatt aagtgaattc tcacaatcga tgttgaatca
    11521 tgtgcaagca caagatattg gtcctgtagg agattcctta actgaactga tgtatcgtct
    11581 ccaagaagcc aatcctgacg aactgcgtgc cggagaaggg aacatcttcc agcgtgtctt
    11641 cgggaaggtc aaacaatcga tctatgaagt gacagcaaaa tatcaaaaga ttggtgcaca
    11701 aattgacaag atttcagtga aattagataa agaaaaagac ggtctgctaa aagataatct
    11761 gatgttagaa cagctttacc agaaaaacaa agactacttt gatgcactga atatttatat
    11821 cgctgctgga gaattgaaaa tggaagaact tcaaacaacg atcattccag aagcgatgaa
    11881 aagagcagaa gaaactggcg atcagatgga tgttcagatt gccaatgatt acacgcaatt
    11941 cctagatcgt ctggataaac gtacgcatga tcttcgctta gctagacaga tcacgatcca
    12001 gcaagcacca caaatccgtt tgatccaaaa cacaaatcag gccttggctg aaaagatcca
    12061 agcatctatc gctacagcca ttccgctgtg gaaaaaccaa gtcgtgatcg cattgacctt
    12121 gcttcgtcaa aaagatgcag taactgctca acgccaagtc tctgaaacaa caaacgactt
    12181 attgaagaaa aattcagaaa tgctgaaaat ctcagccatc gaaacagcga aagaaaatga
    12241 acgtggaatc gtggatatcg agacccttca aaccactcaa aatgatttga tcgaaacaat
    12301 ccaagagaca ttgcgtatcc aaaaagaagg aaaagaaaaa cgtcgtcatg cagaagtaga
    12361 gcttggacac atggaagaag atctgaaaca aaaactgttg gatttaaccc aatagatcac
    12421 ctttcgcttg ttactaaaac aaacgcccaa caacagttta ttacaactgt tgatgggcgt
    12481 ttttcttatc tttcttctaa cttctccatt ggagtctatt tctcattcat cttgctgtgt
    12541 ccatagccat agccaaaata aatgatcagt ccaataacca ttacgatacc gaacacgatc
    12601 catgtcacag tttgcagacg aagcatcaat acaatacaag tcaagatgga gacgattggc
    12661 aatagcggaa cgaatggcac tttgaattct ccagcttttg gtttgccaaa catttttctc
    12721 aacttcagta agcctagact aacaagcatc aaataggcga gcgccatgat attcgttagt
    12781 tccgctaata cattcaatgg gaaaaatccg gcaaagaata cagctgccac accagcggca
    12841 taagtcgctt ttttcggtgt acgatgcttt tgatcgattt ctgtcataaa cttaggaagt
    12901 aagccatcct tgctgacagc aaacagtaaa cgagcaagtg aatacatcat cgagatcgtt
    12961 accgtcagta gcgttaaaat tgcccctaca gcgatcaccg cgcccactgt tccatgtcct
    13021 acatacctca ttgcaaaagc cactgggtca ttgactccta gttcagtata cggaacgatt
    13081 cctgtcaaga tcagcgtcac gatgatgtaa aggacagtag caatcagtat cgaaccaatg
    13141 attcctcttg gcacatcttt ttgcgggttt ttgacttcct cggctgccat tgcaacggca
    13201 tcaaatccca agaaagcaaa aaacacaaga gctgcaccat ctagcacacc ggaaaaacca
    13261 aaaggcatga atggctgcca gttatcaggt ttaacgaaaa atattcctac aatgataaac
    13321 aacgcgatga taccaaattt gacatatacc atggcattat tcaaacgtaa tgctttcttg
    13381 gcttcaagcg acacgatgta agtgacgaat atcactacga gcaccgcaat caagtcaatg
    13441 tatgtaccat tttccggatt gtacccgcca gttaatgctt gtggaagttc ggcccctaaa
    13501 cttttcaaaa aaccttgcat atagccagac catccggaag caacagagga aaccgctagt
    13561 aaaaattccc cgatcaacaa ccaccctgtc agccaggcag caaattcacc aaatacaaca
    13621 tacagatagg catatggccc accaatcact ggcactcgcg aagcaaattc tgcgaaactt
    13681 agtccagaca aaatgacaac gattgcagct aagacaaatg aaagtgacag tgctggacca
    13741 gcattttgat tggccgcaac ccctgtcacc acaaatattc ccgtaccgat gatcgcacca
    13801 attccaagca tgatcaaatc agacgttttt agttcttttt tcatctcact cgtatgattg
    13861 agattgatct ctttcttacg aaacaaatcc attgaagttc ctccccattt taggttggaa
    13921 aacagcgatt aaactgtttt catataccct aaaaggatac cacctaaaat gactaaaagg
    13981 caaccaacaa taacataaat gagctctttt ttcgtttttc tttcgccaag gatgaaaatc
    14041 ccacctaaag tagagataat gatccccatt tgtgaaagtg agaagccaac agctaaacca
    14101 agtgatttga ctgttagaag catgcagatg tttcctaagc cccataataa tccacagatc
    14161 atgtttttcc acacaaactg atcgacttta actttacgga aagcaaagaa gcttgcgcct
    14221 aagatcattc cgatactttg aggtaagata attgacatcg catccaaatt cgcccagttg
    14281 acgatgatgg tatatacacc ataaccaatc gtagagaaga tcaatgctct aaaaccctta
    14341 ccaaaatcaa gcattttatt atctgtatct atttttcctt cgtcatcttg tcgggcagtc
    14401 aaatacgcac cgcttacaag aagagctaaa gcaataatac cctatacata atctttcgtt
    14461 tgtgtccatt catggaagaa aacggctccc gcaatcgtat tcaaaattaa ttgaaaacca
    14521 gtagacaatg gtagccctac agaaacaccc atgtacttca ttccatggaa ctgtccattt
    14581 tgtccaacac tccaagctaa gccggataaa aatccgatca agaaaatcca aggggtgatt
    14641 acaggactaa caacgaaaaa gacaactaaa gaaaacagaa atgcaccgat cgtcattcct
    14701 agtgtttgtt gattggcatc gccaccaatt ttaccactta ctaaaccaat actaccccat
    14761 gccaacatag ggactaatgc tattaaaatt tccataacca atctcctttt atttcacatg
    14821 ataagaaact tatcagcgaa aacgttaaca acacaagcac tttttttcgt ttctaacgat
    14881 tttctaacgt ttttcattat aaaaatttaa gattgggaaa aataaagtca ttgttgtccc
    14941 ttttccttca gttgattcag ccgtcaaatg gatacctaat ttttcggcca gattttttgc
    15001 taaataaagt cctaatcctg ttgaatgtgt tttgctaagt cgaccatttc tcccagtaaa
    15061 tcccttatca aagatccgtt tcaagtcttc cgctgggata ccgatccctg aatctttaac
    15121 tgaaaggctg actccttgcg cttccttttc tatgctcaca atgatgtttc cgtgatccgg
    15181 cgtatatttg atggcattac taagcaattg gttgaagata aaagcgatcc atttagcatc
    15241 ggtcaaaaca gtatgatcct caccaacgat ctcgtagcga atattttttt ggataaaata
    15301 atttgcctga ctgcgtatca caggctgaat gattcctttt agtccatatt cttggatgag
    15361 gtagtcccgc gagaaactgt cgagtcttgc ataatacaaa acttgttcta cgtattcatc
    15421 gatttttgat aattcatttt ctagtaatgt atacttttga tcgtcaatat cgaactcgat
    15481 cgaacgaact agcaatttag aagcggaaag tggcactttg atttcatgga cccatgagtc
    15541 aatataatct ttttggtctt gttgattact gattgctgat tgcattactt cttggtgttc
    15601 acggatcagc tgattgatat attcttctac cagtttttct tcttctgtcc tcccaccact
    15661 caaataattt tgcaaagggg aagttttttg tatgtcggct aattttcccc accatcttct
    15721 cttgctcaag taaccaccca gcagataaaa aatcagaaag acgccctcaa tcaaagctaa
    15781 ataagcaatt gtgctcaaat caactttgat gtttggcgcc agccacatca caaagatcgt
    15841 caatgcaata aaaaacagcc atcccaagat caatgtccac tgatctttca aatatttaaa
    15901 aaaattcatc ttaaccgctc atcctttcag ggaacgatat atccctgtcc tacttttgtt
    15961 tcgatatatc cttggatacc tgcctgttcg atttttcggc gtaaccgatt gatgttgact
    16021 gtcaatgtat tgtcatctac aaatcgttcg tcatcccaca gtgctcgcag caatttttcc
    16081 cgacttaaga tctttccatt gtgcttcatc aaaataaata atagacgata ttcatttttg
    16141 ctcaaatcaa tgatctctcc gttgatttcc atacttccgt tgtcaacatt caacgtaatg
    16201 ccattgtggc tcatcatttc actagataat tctgtgtagt tgtaagaacg gcgaagtaat
    16261 gcattgattt ttgcaatcag tacatcgatt tggaaatgtt ttgtcacaaa atcatctgcc
    16321 cccatgttca tagccatgat catatccata ttcgtattcc tgctggagat aaaaatgatc
    16381 ggtactttcg atacttcacg tattttttga caccaataat agccatccaa aacgggaaga
    16441 ttaatatcta gtaatacaag ctgtggttct tctctttcaa agtctgagaa cacttgattg
    16501 aaatcggtcg ttccaaaggt ttcaaattgc catttctcaa gttcctccat gatcaattgt
    16561 cgaatgatct cttcgtcttc gactaccatg atttttgcca taaaaaatcc ctccatcttt
    16621 cttcttgcta ctacaatatc aaagaagccc ttgtgaaaca agttggctta cctggaaaat
    16681 gtcattttag caaacgttaa aaaaaactgg aaatatgcga tttaatcgcg tttgtctctt
    16741 ccaagcagct taaatttatt atgttattct taataattgg tctattatct gttatttttc
    16801 tttcgttgtt tttgtctttt aaattttcga taacaccaat aaccgaaaag cgttagcact
    16861 agcaatatac ctagaaggag tataagtaat cgataaagat ggtatttttt tgtctgttct
    16921 atctttgaag agacttcttc tgtaaaggga actcgtcttc ctgtcactag taatcggtga
    16981 gtgttcacgg tgtaaggtgt acaagtgata agtgtgacta gatcctgctt ttctctaatt
    17041 gctaatgtat ttgtttttgt tggaaggaca acaattttct caacgacttc ataagcgagc
    17101 atccgaccat acacttcaag atagaatcgg tcatcttgct ccatttcttc aagatccgta
    17161 aagagttttt tctcagcaat accgctatgg gcggtaatca cggaatgtgt actctctcct
    17221 ccagttggaa aagaggtgcc ttgcaggact gtcgctcctc tatctaacaa aagatcattc
    17281 gtttcatcaa ataaaggcaa gctaacattg attttcggaa tatagatggc tccgatcata
    17341 tgttgttcgt aatattcttt tcctggattt tggacatttc taaccgcttg ctcaaaagga
    17401 tctttgactt gtcccattcc tgggattact gatgtatgag caagcgtctg atttttcttt
    17461 tcatgcactg ctaattgttg ctcatgtttt tcttctttat cgcgttcaag ctgcttttga
    17521 taattctcga tccttctttg atccaaataa ttgctgagag catctgctaa aaatggatat
    17581 gtaaaaactg cagctccggc aagaaacatc actgccataa ataattgtaa taaaagactg
    17641 atttgggttt gaggtctatt acctattttc tttttcattt tcttcacctt cttatttaaa
    17701 aaaactgcag caacctttat aaaaccgatt gctgcagctt atttttgttg cttatacttc
    17761 agacttcatt ttagatttac ggtaccagct gtacgctcca atcatcaaac ttattccgat
    17821 tagtaagaat gccaagagcc cgttcccacc agtagcagga agtgacccgc gtttcatatt
    17881 cggtactctt tctggccctg gattttctac tcctacgatc tgaatttctt cattatagct
    17941 tccataagag atgataaatt cagtaaaggc tgcctcacct gttggaagta cataaccttc
    18001 tggcgccatg gtctctttca tttgataggt tccataatct aacccttgaa cctcaaagta
    18061 tccatttcca tctgcgacaa attctgttgc ttgtccgctt gttgcccact caattctatc
    18121 tgctcctgat tcataaacag ctaaaggcga ctccccttta taaaatattg cgtattcaga
    18181 acggtcttca ttccacaatt cgaatcttgc atctttaagc aattcactgc ttgatgcatc
    18241 atatttttca aactttcttc cacctgttgt gactttctct ggcggctcta ctccttcttc
    18301 ataatcaaat tcatcatttc gtccgatttc aataaccgct gtgttgttat ggaaatcatc
    18361 cgcttctagt tctgtggaaa ccttcaaatt atacgtgatc gttaaatctc ctccacgtgc
    18421 atctgctaat ctacctaaag tatctacttt ggctactcca ttttcttctg tcaaaatcgt
    18481 cacgacaaac ccgttgctta ctacttctac tatataatct tgtccttttg ttaaaatcgt
    18541 ctcgccggct cgcacttcta tttcctgatc ataataagtt agaccaactg taggcgtatc
    18601 tgtgattttg taatttttga taacagcgct tccgttttca aggacagaat cgatgaaata
    18661 aggaataggt gctgtgatcg tgtaagaagc cgtttttcct ctttctagat caatataaga
    18721 aatcgtttct tgttctccag cttcattaat aatcgttact tgtctaaggt cgctttcctc
    18781 tacattcatc tgtttatctg cttctctgat ttcgtttttc ggatacaaat gcacatctgt
    18841 attgtatgtg tctttccatg tagaaccatc ccacacgcca tccaccatat caggcatcat
    18901 cactggcatc ataacaacca ttggagacgc gattctggaa atatgagctg gcgaataagt
    18961 ttctaagaaa agatagactt tatctctaga agaaggtgct tcttccgtca cttggacggt
    19021 agagaatacc gcttctccag cttcattcgt tgtttgtttg tcaatacttg tcagtcgata
    19081 ttttaaaaac caactttctg tgtgccgttt ttgaatccat tcaatcgctt cggcagaatt
    19141 aagaccgtct tctcgttccg gtgttaaagg attatctcga atcaaatcgt aatatacttc
    19201 cgttacgtca aacaaatcaa aatcgactcc tggaatattt tcttcaccaa aatccgtaga
    19261 aaccaatccg ctattcggtc gttcttccgg aacagatgag aaaccttttt tgtgtaatgt
    19321 aatcgtcact tcacttggtt tttgtgcgac ttcttcggct ttgactagtt ttccgttccc
    19381 gaaaatccct attcccgcag taacgagtaa cagtacactc attcccgtga caatcatttt
    19441 tttattcatt tttttctccc cgttcttttt ttatt
//
LOCUS       DACDTO010000051        19130 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDTO010000051 DACDTO010000000
VERSION     DACDTO010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.051"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IWK70_001794"
     CDS             complement(<1..703)
                     /locus_tag="IWK70_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9614321.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IWK70_001795"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IWK70_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IWK70_001796"
     CDS             1758..2837
                     /locus_tag="IWK70_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9614322.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IWK70_001797"
     CDS             2869..3462
                     /locus_tag="IWK70_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614323.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IWK70_001798"
     CDS             complement(3572..3799)
                     /locus_tag="IWK70_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9614324.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IWK70_001799"
     CDS             complement(3902..6490)
                     /locus_tag="IWK70_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9614325.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IWK70_001800"
     CDS             complement(6510..7169)
                     /locus_tag="IWK70_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9614326.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWK70_001801"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWK70_001801"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9614327.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IWK70_001802"
     CDS             complement(7942..8808)
                     /locus_tag="IWK70_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9614328.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IWK70_001803"
     CDS             8917..9558
                     /locus_tag="IWK70_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9614329.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWK70_001804"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWK70_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9614330.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IWK70_001805"
     CDS             complement(10999..11898)
                     /locus_tag="IWK70_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9614331.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IWK70_001806"
     CDS             complement(11900..12697)
                     /locus_tag="IWK70_001806"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9614332.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IWK70_001807"
     CDS             complement(12675..13379)
                     /locus_tag="IWK70_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9614333.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IWK70_001808"
     CDS             13510..14085
                     /locus_tag="IWK70_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9614334.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IWK70_001809"
     CDS             14203..14880
                     /locus_tag="IWK70_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9614335.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWK70_001810"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWK70_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9614336.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IWK70_001811"
     CDS             complement(16943..17941)
                     /locus_tag="IWK70_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9614337.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IWK70_001812"
     CDS             complement(18167..18820)
                     /locus_tag="IWK70_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9614338.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4137 c         3324 g         6144 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DACDTO010000052        19030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDTO010000052 DACDTO010000000
VERSION     DACDTO010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.052"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            145..1572
                     /locus_tag="IWK70_001813"
                     /pseudo
     CDS             145..1572
                     /locus_tag="IWK70_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1664..2029)
                     /locus_tag="IWK70_001814"
     CDS             complement(1664..2029)
                     /locus_tag="IWK70_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9614339.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(2026..2391)
                     /locus_tag="IWK70_001815"
     CDS             complement(2026..2391)
                     /locus_tag="IWK70_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9614340.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2583..2831)
                     /locus_tag="IWK70_001816"
     CDS             complement(2583..2831)
                     /locus_tag="IWK70_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614341.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2830..2958
                     /locus_tag="IWK70_001817"
     CDS             2830..2958
                     /locus_tag="IWK70_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9614342.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            3015..4709
                     /locus_tag="IWK70_001818"
     CDS             3015..4709
                     /locus_tag="IWK70_001818"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9614343.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4776..4982
                     /locus_tag="IWK70_001819"
     CDS             4776..4982
                     /locus_tag="IWK70_001819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614344.1"
                     /translation="MFLIWLDLLPQMQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFL
                     TWLDLLPQMQAFEHRLPRGYEKGV"
     gene            5360..6493
                     /locus_tag="IWK70_001820"
     CDS             5360..6493
                     /locus_tag="IWK70_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9614345.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            6524..6844
                     /locus_tag="IWK70_001821"
     CDS             6524..6844
                     /locus_tag="IWK70_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614346.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(6873..7337)
                     /locus_tag="IWK70_001822"
     CDS             complement(6873..7337)
                     /locus_tag="IWK70_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9614347.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(7469..8722)
                     /locus_tag="IWK70_001823"
     CDS             complement(7469..8722)
                     /locus_tag="IWK70_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9614348.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(8751..9068)
                     /locus_tag="IWK70_001824"
     CDS             complement(8751..9068)
                     /locus_tag="IWK70_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9614349.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(9094..9414)
                     /locus_tag="IWK70_001825"
     CDS             complement(9094..9414)
                     /locus_tag="IWK70_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9614350.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(9878..11293)
                     /locus_tag="IWK70_001826"
     CDS             complement(9878..11293)
                     /locus_tag="IWK70_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9614351.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(11310..12626)
                     /locus_tag="IWK70_001827"
     CDS             complement(11310..12626)
                     /locus_tag="IWK70_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9614352.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(12776..13474)
                     /locus_tag="IWK70_001828"
     CDS             complement(12776..13474)
                     /locus_tag="IWK70_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9614353.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(13763..14752)
                     /locus_tag="IWK70_001829"
     CDS             complement(13763..14752)
                     /locus_tag="IWK70_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9614354.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(14832..15665)
                     /gene="msrA"
                     /locus_tag="IWK70_001830"
     CDS             complement(14832..15665)
                     /gene="msrA"
                     /locus_tag="IWK70_001830"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9614355.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(15840..17474)
                     /locus_tag="IWK70_001831"
     CDS             complement(15840..17474)
                     /locus_tag="IWK70_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614356.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(17461..18861)
                     /locus_tag="IWK70_001832"
     CDS             complement(17461..18861)
                     /locus_tag="IWK70_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9614357.1"
                     /translation="MWLLNICTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIY
                     YRSAIAEAIALTIIPAVLYYAYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIA
                     CMIIVRLFLSKQIKNDIKFVVVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTD
                     LQLEALSISDSIIQALNNDITTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTW
                     VCVTNLFPWFLFQKTPISLLQFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGA
                     TVVFLLVTSIAAKENYAKVIQSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERP
                     HLEKHEVKVGESWEKGKYKAADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVND
                     SYKKHGLVNVDVNAGENKIEITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKD
                     SRYVTKKEINKKNENK"
BASE COUNT         6039 a         3735 c         3171 g         6085 t
ORIGIN      
        1 tctgttttcc aaattttata acataaaaaa ccttttttaa cggcatttat atgctttttc
       61 agaaattaac tgatgatttc aaaaagaaaa catgtcataa tatttcccat attgtgcaaa
      121 aaaatacaag gcggaggctc tactatgatt gaagaatata ttgagaaaaa tattttaaga
      181 cagctgtttt tatgtgggca attctatgta aataaagaag tcaatctaga aaagctctcc
      241 aatctcctgc atgtttgcaa aaccacttta ctcaatgata tcaataacat aaaaaaagaa
      301 tttgaagaac agatcgctta tacacatcgg gaaaaggacc gttacacttt atatttttca
      361 gaacatatac ctcgatgcaa gatcatgcag caattgtctc aaaattcgtt gtttttaaaa
      421 acttgtctgc tttatttgga agaagatgaa cctgattatc ttcaattaac tgaatgtgaa
      481 ttcatctctg tcagcaaagc ttattcatta aaaaaacaag tattagctta ctttaatgac
      541 tgcgggatcg aaatcgaccg ctactcccct cgattcactg aaatggaaag acgtctgctg
      601 cttcttaatg tgtcgtatcg tttaggcggt tttaattctt gggagctacc tgaatcgttt
      661 tttgaacgtg ccgatcgttt cattgaatcc gtaacggaaa atagtggcag attttatgat
      721 aaagaaaata aagaaatctt atcgattggt tttgctatca gtttcctacg ccagcaagtg
      781 tgtgctgtga caatagattc aaaatttatc gaagaaatca aaaaacgtcc tatttacaat
      841 tatgtcgaat cagcatggga aaacactgat ttccaaacct attataaaaa agaagaattc
      901 gcctttattt tgacactatt caatctttgt aattatggtt tccattctta tcagttgatt
      961 gcggaagact ttcaacagct acaccaagtg tttattgaca atactcccga aataaaagaa
     1021 ctagttgcta cctttgaatc tcacttcaat caagaattat tcggaaacca accgttcgaa
     1081 cgtgcgctga ttcatctgat gcgttcggct tgggataact atcaattatt catgccagaa
     1141 aaattttatc ttttaaatga ggaacagaca aatttatata aagaagtcca gacaattttt
     1201 tccagctggt ccagccagtt accttatgat ctacggttga atcctaattg catgcgggct
     1261 tttgtgatcg aattgagcgg aattctccgt ctgacaaaag aacatctgac tatctatatc
     1321 gtcacaaatt cggatgttca ttatctgatt tatcgggaag ccttggaagc tgtaactact
     1381 ttcgattttc aagttgcacc aacaatttat tcaagtatat ctgatataaa gaagtatgcg
     1441 cagtagtcct caaaccgggt attatgtgaa cgtacattat atacaccaga tgcggtccaa
     1501 tatgaaaata ttattcctat ttcgatcaat acgatcgatc gagctatcat ttcagctgtg
     1561 caaaacaaat aaggggctgt gacaaaaatc atgtcacagc ctcttatttg ttgaagatgc
     1621 ccatttctgt aacaatctct tgatacacgt caggctcaca acctcactta ttttggacat
     1681 ctaggggtcc gatctcttta tttaaaaaat aagaaatgat ggttcggata actaccaacg
     1741 ccgctagttg gaaaatatcc tgcaaggtag gtttgataat cgattcgatg atcccagctg
     1801 aaacaagaat ttccaaactt agaagtatat agcctcccaa catcttttta atagcattgt
     1861 tctcgcttac tcggtcgctt tgcacttttg tatggacaaa taaatatttc aaaaaatcct
     1921 tgattgatag acaaacaccc caaaccaata aacagatagc aaagatatca agtcccagta
     1981 caagtttttc gaaatatgga agaagaaact gcatcaactc tgtcgtcatc ctacctctcc
     2041 tcttctttgt ttttctttgt cttctatttc ctgattcaag aaataagaaa tgactgttcg
     2101 aatgataata ataagtgcaa gcttcaaaat atctgtccaa gtcggcttga taactgactc
     2161 gatgatatca gcaacgacta gtacttccaa gcttaacaaa atataacttg ctagcagttt
     2221 tcttgtatct gcattacgag tattacgttc tgcatagttt ttcttgacat gagaccaatc
     2281 aaaaagtttg aatagaacaa gaacgatccc gacaattaag ataccgatag aacaaatatt
     2341 taaaatagca atgatccatt ctagaaattc aattaaattt gagcgaaaca tatacgctac
     2401 ctactttctt ttaatttgtt cctattcttt tattgtaaaa gaaagttacc ttgttcgtct
     2461 atttaaaagt tgttctaatg ataacggttc gcttaaattt ttctttttca ctctcccatt
     2521 tttctccctc catccataaa tcgtggaagt atccaaaaca acgtagcaag ttaaaataag
     2581 gcttagtccg tcatcagctt ttcaaagaag cactgtctcg gtgctttttt tgtacccaaa
     2641 tttctaaaaa tagaaccata cacctcctca aatcttttct tcttacctac cctattttta
     2701 tttacaggaa aatcagacac ttcttctttt ttgtcaaaat ttcctttttc ttctcgtcct
     2761 atcatttggc attctaataa aaacgaacta tccttggata tagaaagaga aaacaaggag
     2821 gcgtttttca tgagtaaaaa attaatcaaa gtcggcatcg gtttaggttt gcttgccctt
     2881 ggcgctgcat atctaggaaa aaagacagga ctattcgaag atgacagtca tttatatgat
     2941 gaattcgaat caatctagca tcctatttag ttgaatcact acggacatac ttggaagaca
     3001 ttggaggaat cacaatgaga aaaagtaaag caataatgat tggtgccggt attgccaata
     3061 tggcagcggc tgtttatttg attcaagaag gcaaatggaa aggggatcag atcacctttt
     3121 attcattgga tgatcacggt tcaaatgatg gtgcaccgac cgacactgta gtggatgaat
     3181 attggaataa aaaccatcca ttagaaaacc aaaaaggcta tattgctcga ggcggtcgca
     3241 tgcttaatta ccgtacctat gtagatttga tggatctgtt aagtcgaata ccttctgtta
     3301 ctgagccagg gatgactgct gaagaagata cgagagattt tgatgcaaaa caccgaacct
     3361 ttgacaaagc acgtttgatg gaaggtggca aaggaatcat tgatggcgga aaactgggat
     3421 tcaataataa agaccgtatt cttctgacta aactgattgc tatgcctgat tcagaagaag
     3481 aaaaactaga caatatcact atcgaagaat atttcaaaga tgatccacac ttttttgaaa
     3541 caaacttctg gtttatgtgg gaaacaacat ttgctttcag aacacgtagt tctgctcaag
     3601 aattgcgtcg ctatatgcat caaatgattt atgaattcac tcaaatcgaa catctagtgg
     3661 gcgtcaatcg aacacgttac aatcaatacg aaagtattat gcttcctttg atcgagtact
     3721 tgaaagaaca aggatgcaaa atcattttga accgccgtgt cactgactgg gaattcaaag
     3781 aaacaccgat gcaagatgaa atcactgtga ctggtttgaa aattaccaat acactgactc
     3841 aagaagaaga atacgttgtg gtcgatgaag atacagcagt tattttcact aatgggtcca
     3901 ttacagattc agctacttta ggagattacg aaacaccagc tgtagaaaat atggattatg
     3961 gtgcagcttc tagtctctgg aaaaaagcca gtgaacgttt ctacaacttg ggaaatcctg
     4021 acaaattctt tgctgatcga gatgcaagcg aatgggtcag ctttaccctg acgacgaaaa
     4081 accatttgtt gttgaatgaa atcacccgta tcacgactca agaaccagga aatgccttga
     4141 attcatttat ttcaaccaca ccgatcacgc cgttaggcca aaaagatgtc aatatgtcta
     4201 ttgtcgtgca tcatcaaccg cattttacgt ctcagaaacc aaatgaaaca gttatttggg
     4261 gttatttcct ttatccaaga cgacgtggcg aatttgtgga taaacagtat attaaaatga
     4321 atggaaaaga aatgctagaa gaactgattg gacaactttc aaaagtagat cctggaccag
     4381 tgaatattcg tgaaaaagaa acagaaattt tcgacagcgt aataaataat atccctgtct
     4441 atatgcctta tgcttccgcg ttgttcaata atcgtgccaa aagcgatcga ccaaaagtca
     4501 ttcctaaaca ttcgaccaat ctggccttta caggagaatt tgtcgagcag ccttatcaga
     4561 tgatctttac tgaacaaagt gctgtacgtt ctggagaaat cgctgctttc cattttgcgg
     4621 gtattcctat gtcccgtctt gtcaaaacac cgcgttacga caaagatatt cctactctag
     4681 ctcgtgccgc aaaaaaaatg tttgaataat caagaggttg tgaaaaagct gccctgcatc
     4741 aagtaataag aacaaccagg ctaaaacagc ttcttatgtt tttgatttgg ttggacttat
     4801 taccgcagat gcaagctttt gaacaccgtt taccaagagg ttatgaaaaa gctgccctgc
     4861 atcaagtaat aagagcagcc aagctaaaac agcttcttat gtttttgact tggttagact
     4921 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttatgaa aaaggcgttt
     4981 agtttcgagt atgaagaaaa attttgtgaa aatggttcct cacgtttttg cataaatttg
     5041 acttaatacc gagaaactga tcccttctgt cataacctct ttttatttat gtctgtttct
     5101 ttttgaaaac aactattttt tcaacgttta ctctcttgcc aatccaacat catctaacgg
     5161 atacaactat ttcaatgatt accattacac taaaaaatcg gaaacacacc tctccgaaca
     5221 caaatacata agactataga atgtttcatt taagaaaaaa agcgtttcat tatcaaaaag
     5281 gtaagatctc ctttaaaatg aaattatctg tagtactaat aatgcagaca attttatgaa
     5341 tctgaaagga gcatctccaa tgaaagcagt cacttggcaa ggaaaacaga aaatggaaat
     5401 cagaaacgta gatgatccaa aaatattaga accaacagat gcaatcatcc gtatcacagc
     5461 aacagccatt tgcggctccg atctgcatct ttaccatcat ggtgaagcag ttttggaaaa
     5521 agggtatgtt gtaggacacg aaccaatggg aatcgttgaa gaagtgggat ctcaagttca
     5581 aacattgaaa aaagggcaac gtgtggttat cccttttaat attagttgtg gtcattgtca
     5641 tttctgcctg aatcacatgg aaagccaatg tgatgaatca aatccagatc agttatatgg
     5701 cgggcttttc ggatttggca agttgaacgg taatcattgg ggtggtcaag cggaatatct
     5761 aagagttcct tatgcagact ctacttcatt tgttgtacca gataatgatc ttcctgatga
     5821 aaaagtttta tttctatcag atattttgcc cacagcttat tggagcgttg aaaacgcagg
     5881 agtaaaaaaa ggcgatacag ttgtgattct aggttctggt ccagtcggtc tattcgctca
     5941 aaaattcgct gttatggctg gagcagagcg cgtaatcgca gtagatccag tacagcaccg
     6001 tctagataaa gcggctaatt acaatggggt tgaaacttat ttcttagaag acacggcaca
     6061 agctggaaca gaattgtatg aactggctaa aggtggcgca gatgtcatta tcgattgtgt
     6121 aggaatggac ggattagagc ctgtcaaaga aaaagcgaaa aatttagtaa gcttacaaag
     6181 cggcacaatc tctccattac agatggcatc gcaagctgtc aaaaaatttg gaacagtcca
     6241 agtaactggt gtttatatga ctccagcttc ttcttacccg ctgcaagaat tttttatgcg
     6301 ggatatcgct gtcaagcatg gacaggcacc aattatccat ctaatgccaa aaatttacgc
     6361 aatgattgca gaaggactat tcgatcctac acaaatcatt actcattcgc tgccactatc
     6421 agaagcagca caagcatatg agatctttga caaaaaagaa gataaaaata taaaagttgt
     6481 tttgaaacct tgaattcagt aaaaaataca ggtgattttc tttatgccca aaatcaaacg
     6541 aaatacccat gagatcgaaa taggaaaaga agaagatatc gaaatacgag gacgtcgctt
     6601 ccgcttctat ttccaaaatc gttacacatt gatctcactg gctgtcgatt tattaaccgg
     6661 gattttctat atttttggaa gccttgcttc cttaactaaa atccccgatc gatatggcac
     6721 ctatttttat ttggctggtg ctattttctt gacgattcgt cctattctca gaatcgtaag
     6781 gaacattttt atttacgacg aaaaaaaagc aatgaagaga acaagcaaaa aaaaagaggc
     6841 atgagaattt ttcaaatctc atgcctcttt tttcacccta tcgtatcact ttgatacaga
     6901 cgagcggtcg tcccactttc ttccttgacc attgtcaaaa atgtccactc gtatcgttca
     6961 taaaacgtgg tatgatctgt aattaagtaa agtttttcgt acccaagttg atgcatttct
     7021 tttctgattt ccgctaatat ccgcttggct atcccctgct tacgcttttc cggttcaaca
     7081 aacagggcac aaagatttgg tgtcagatca gggcggttat ggaagtcgtt ttcaatgacg
     7141 cccgctcctc cgacgatggt ttctttttca tctaagacca cataccattg aggaatactt
     7201 tccttattag cgattccagt acgaatactt tcctcataca caacagcagg aactccccat
     7261 tttgatgaaa accaattcgc gctttgtttt actaattctg ggtgttctct taatttgatc
     7321 cagttatatg taaccaatgt ttaaacctcc gactaaaata aaacgtttac aaaaattata
     7381 caagtcttta tgaaaattag caatgacaac aatagtgatg taaaaaaaaa aacggaaaac
     7441 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
     7501 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
     7561 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
     7621 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
     7681 caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca
     7741 cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca
     7801 agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta
     7861 aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca
     7921 cgataaacgg tgcgctatgt ccagctgcga ttgcattaac gtttgcttca atggctggcg
     7981 cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata
     8041 agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa
     8101 atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa
     8161 aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa
     8221 atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc
     8281 cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac
     8341 cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac
     8401 ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc aagaaagcaa
     8461 tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat cctttccatg
     8521 attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt aagttattga
     8581 tcattactgc taatgctcct aagatcatta acggcatact caccataaat gagtcccgaa
     8641 tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa tgtttctcca
     8701 taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga ttacgcgttc
     8761 aatcgacgat acagattaat gatctctttt gctaaatctg taaaagctat agatgtcatc
     8821 agatgatctt gactgtggac cgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct
     8881 tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc tgcatctttg
     8941 attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc ttccattgca
     9001 tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag atttttttga
     9061 tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt tttctgcttg
     9121 gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta catctaaagg
     9181 aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc
     9241 taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg aagcagatac
     9301 agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt ttgtgactag
     9361 gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt ccattttaaa
     9421 cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac tttaaaagcg
     9481 ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac acactaaaaa
     9541 agaaagcgca cactaaacac acaaattaat ttttattacc ttttttcttt taacgtgcac
     9601 aaaaaaagtt gtgacacaaa acttgtcaca acttttttca aataaacggt attcaaaagc
     9661 ttgcatctgc ggtaataagt ccaaccaagt taaaaacata agatgttgtt tttactttgc
     9721 tattcttatt acttgatgca ggacagcttt ttcacaacct ctcaataaac ggtgttcaat
     9781 cagctgaccc tgggtattag ctcaaaacgc tggattcaca acctctttta tttcaaataa
     9841 ccagtaaata caatgaacat ctacaaaaaa cttctattta tttatagatt tcgtcaggca
     9901 aatccaattc tctcttatca atcaaagtct gataccacga agcagatttt ttcaatgttc
     9961 tgcttcgttc atcgttcaat tcgattcgaa ctaatccata tcgattctta aatgcattca
    10021 ttggtgacac acagtcagtg aatgcccata acatatagcc ttcgcaattt gcgccttctt
    10081 ctgttgcttt gatcaagtaa tgcaaatgct gtccgataaa ttcgatacgg taatcatctt
    10141 gcacgattcc tttctcatcc atatactgtt cttcatattc tcttcccata ccgttttctg
    10201 ttatcagcca agggatatta tgataatttt cttttagata catggctata tcatacatga
    10261 ttttcggata gatttcccat cccctggaat tattcatttt cttcccaggt aaatcaaaga
    10321 cttcataata tcgttcagga tggaaaggtg tctcttcatt ccattgataa cgaggggcgc
    10381 gaacgcgttt aggataatat tgattgattc caaggaaatc tactgtatga tgctgaatcg
    10441 tttctaaatc ctctgttgca ggatcgaaat aaatcgattg tttttgacac aactccagga
    10501 actcttctgt gtagtatcct tttaccatcg gatcaaaaaa tactcgatta taaaacaaat
    10561 catacatata agcagctttt tggtcttctg gagaattgga tcgagcatag accatttcag
    10621 gatttaaaat acagccaatc cttccaccat aatttccgct gcggtaaatc tctactgctt
    10681 tagccgttgc taaaacttta tgataattcc acatcatcca cttttttgtg ttttgttcat
    10741 gcggccagcg aacagcatcc aaatagcaac gcgtttgggg aacgattggt tcgttgaatg
    10801 taaaccagtt ttttacccta tcaccgtaac gatcaaaggc aatttgagca tacttagcat
    10861 aaaaatcaac tacttttctt gaagaccagc catcatattt ttccaaaaga aaagctggga
    10921 cttcataatg ttccaaacaa atcatcggtt ctacgtctgc ttcgatcaaa gagtcgatca
    10981 tttcacttat atgctgagca tattcttcat ctactataca gttctcataa tcagtaaaaa
    11041 aacgagccca attgatagaa gtccgataat gtgtcagatg gatggatttc atcagatcca
    11101 catcctgctg gtattgttcc ataaaattag ttgctacggc tgggccatac ccattatgcc
    11161 aaacaaaatg ttcattttta taccagctat ctataaacga atcctgtcct tctttttttc
    11221 ctttccaacc ttccgtttgc caagcagaag atgcagcacc aagaataaaa ttttttggaa
    11281 tagtaatttt catgatttcc tcctgttttt tagatcccat cattttccac acgatttgct
    11341 acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag ttgcgtaata
    11401 actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg cattggccaa
    11461 gggaccatta caactgcttt cgccgcaaat cctaaagcgg ttgcaaaata accaatcgtt
    11521 ccagtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag catgatcggc
    11581 ataccaaaaa taactggttc attgatgttg aagatcccag gagcaaggga taacttggtg
    11641 atttgtttca tatcttcccg ttttccaact aacaatactg caatcagtaa accgatcgtg
    11701 actccagacc cgcccatact catatacata tcccaaaatg gcatagtaat aatgttcggt
    11761 acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa aagcaacggt
    11821 tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa gacttgcgca
    11881 ataaacatca atagcaaaat acccggtaac ccttgtacga caccttctaa tgggcgttgt
    11941 acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat gataaatccc
    12001 gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa actagcagaa
    12061 acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt cctcaaccag
    12121 caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc tttcgtatct
    12181 gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt cactaattca
    12241 taaacagttg gattaacaga taaataagac atcactgcga tgataccagg gaatacacca
    12301 tccactttat tcagcttggc taattcgatc cctatcagaa aaacagcata gatggtcaag
    12361 aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc taaaaaacgc
    12421 aagaaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga aaacaatgtg
    12481 ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt tatcaccatg
    12541 atatacctta aattattgat tctcgttgcc acgttcccta aaacattggc taatttatcc
    12601 ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca tctctttttg
    12661 tctattagtc agcgcttaca tttcgatgaa cagaaactat ttactcctct tttttaccaa
    12721 atataaaaca aaaattcatc tgatgtattt atcataaaat acatcagatg aactgtcaat
    12781 tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc atatggacag
    12841 tcattgcatc aaacgcagca agtgcattcc gcctttcgat ggcctgaacg atctctgtat
    12901 gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta aacaaagcaa
    12961 ttgattcatt aatgattggg ataatatgca gcaaggctac gtttccgcta gcttctgcaa
    13021 tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct cgaaaagaag
    13081 tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt gcatattccg
    13141 ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag tctcgtatca
    13201 tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc cctcgcttat
    13261 cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa gctagagccc
    13321 gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat tcatattcat
    13381 tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg atcttgtcag
    13441 tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt tcatctgatg
    13501 tattttttcg taattattat aacacgtttg aatcatctga tgattttttg tatacaaaat
    13561 tgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa aagactagga
    13621 taaaattcac tcctagcctt cgatcatttt tattttgaac gacaaaaaat atgatgttca
    13681 ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta atatatggat
    13741 aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat tcttcttctg
    13801 gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt aaccgacttg
    13861 ctccgaccag tacacttgta acagattttt gtcgcaaatt ccatgcaagg gccatttgcg
    13921 ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt tccagagttg
    13981 atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta gaatcttcag
    14041 gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg aacgtgattg
    14101 cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt gcacgagaaa
    14161 acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt aaaatttcag
    14221 tcattttttt tgtatcttct ccattatagt tggatatacc aatatataat gcttttcctt
    14281 gtcttacaag ttgcatcaaa gcttctgctg tttcttccaa aggtgtatca gggtctgggc
    14341 gatgatgata gaagatgtca acataatcta gccccattct ttggagactt tggtcacaac
    14401 ttgaaatgat ggatttacga gagccccatt ccccatatgg cccttcccac atatgataac
    14461 ctgctttgct agaaataatc agttcatcac ggtaggcttg aaaatcttct ctaaaaatac
    14521 gcccaaagtt tttctctgca gctccagcag ggcctccata attattcgca aggtcgaaat
    14581 gggtaattcc catgtcgaat gcgcctctta aaatctctct ttgattatgg ataggatcat
    14641 aatcaccaaa attttgccat aatccgagag ataaaatcgg taatttcaaa cctgaatgac
    14701 cggatttacg atattccatt ttttcataac gtgcactatc tgcaatatac aatttttctc
    14761 ctcctcatca aatcttttta aaacgctttc ttttttctat ttttagttta ctacatttta
    14821 aagaaaagga gttaatcaaa atgatttttt cgtatatttt tttgccattt gttttttact
    14881 cgttgataga tcagaaattg attacgtgct tgctggattt ttttataacg tttcggttgt
    14941 tttttgtaaa attgctgatg atagttttct gctggccaaa acgtaccggc tggttcgatt
    15001 tttgtgacga ttggttggtg gaaagttcct gaactgtcta gtttctgttt tgattgttcc
    15061 gctaattctt tttgtcgttc gacttgataa aaaatcactg gacgatattg cttccctcta
    15121 tcttgaaatt gtccaaatgt atctgtggga tcagtaattt gccaatagat tgtcagcaac
    15181 tcgctataac ttatttctct tgtgtcataa atgatctcca cagcttccac atgacctgtg
    15241 taaccaccac ttacttgatc ataatttggt ttttcaacat gtccgcccgt gtatcctgac
    15301 aaaacagata ggatcccttt tttcgattca aacggctcga ccatacacca gaaacatccc
    15361 cctgcaaata ctgcacgttc ttgatagtca gcatcttgca tggcatgctt ggaaaagtca
    15421 tatttttgct ccttatcaaa tccttccgta atcaataaat aaaagtccat tacatcaggc
    15481 gtcagattca ttcggatagc taatggacgt agtgttgctt ctagttttga taactgttct
    15541 ttaacggata tattttctct tatgtcattc tttgtctgaa tcaatacttt tcgttcccaa
    15601 tctcgtgtat tcggattcaa aatcagatta taaagtgttt gaatgaatat ttgttcctca
    15661 tccatagatt ctcctccttt ccgaagaaga caagctagtg actttagctg taatttatca
    15721 taaagtgagg aaataagcga ttacttcatc cccctacaga tttttcgtat aaaaaagaga
    15781 gaatccttat cgacagattc tcctttttaa tcaatttatt ttataaatga aaagttaatt
    15841 taccttttat ggaaaactat tttaaaaaat aaacataaat atgcagttaa tgtaacaaga
    15901 taagaaataa tagtcgttac tgtataactt gtgcttactt taatatcagc aactcctttg
    15961 actccgctta tttgtaccaa tccattagca ttcacctgct cctttacagg ttgatgattt
    16021 atttctacat tcgttcctaa ataagcatat acaggaagtg tgattacttg gctcttctca
    16081 ttagttactc tatatatgat agaactacca gttgttttat aaggaaggtt tacccattga
    16141 ctgccaatct ttgcttcgtg ttttattata tgctccttat tctccaatga agcgatagga
    16201 gaataatcat ataaaaaata atccattcct ttagtggtat acgcctctac tgtttctttg
    16261 tttacaacaa tatgtcccgg ctgagaaatt attttttgat tgaaattttt tttactagaa
    16321 acagttaaag tgattaataa caaaacagtc ataaataaat aaaatagttc tcttgtctta
    16381 ttgtatcttc tatttttgaa aaatatcatc gttaatatca gagaagagaa tattatttgc
    16441 aagcttaaga tccgccaagg aaattgtaac aatttggctg gcgtattttg cagaagatac
    16501 catggcacca aactagttgt agctagccaa gtcaaacacg taccataata aatctttttt
    16561 tctatgtttg ttaatttttt aaataaaatc aggggaatac ataaagataa cagaccaact
    16621 agtcccattg agtaagtagt taagtcgttg ttcatagctt cagtcagact atcgaacaca
    16681 ttagatgcat gagcatataa atttgtcacg ctaggtttgt tgattttttg gtagagcatt
    16741 tgttcaaaca tcggatacca aaaagcactc gtcagaacca atgtcattcc tgccgcacta
    16801 aataattttt taaatatagc aataaaatct gcattcttga aacctttaga aaccaaacga
    16861 ataaaaataa aaatgataat taatgctgta ctcattaatg ttgatagtac atggctgtac
    16921 actagcaaac tcatactcaa tgctagcttt acagacggat acttttcttc ttttccagag
    16981 atgatttgat aagcataaaa taatacaggg ataacaagtg ttattgcgat agcttcggca
    17041 attgcggaac gatagtagat gtctactgtc cgataagcag agaaaatata gagacaacta
    17101 aataaaaatg ccgctttttt ttgttttaaa aaccgctcgc cataatgata agctacgagt
    17161 gttgtacaca agttcaataa ccaaacgtaa ataatatagc ttacaatcaa attttttgtc
    17221 atccaatata acattacagc tggaaaaaaa gtcagaaagg gataaaaata atttacacca
    17281 tatccagtat aattgaatgt ctcataatta atcggactgg taaaaatagt cgataacccc
    17341 ttgattcgag ataagtgaaa agaaagatct ccagtggtat aaatactctg tgtatttttg
    17401 aaaaagatga aagaatatat actactaact ataattaatc cactcaatat tactactttt
    17461 ttatttattt tcattcttct tgtttatctc ctttttagtc acatatcgag aatctttcaa
    17521 cttattaggt gcaaatcgtt taaaacttga atacaccact aaacaataag tcagaatact
    17581 gatccataaa gccatgagag cagttggagt atattttgtt gtaatttcta tcttattttc
    17641 tccagcattt acatccacat tcactaagcc atgtttttta taagagtcat ttacgacttt
    17701 accgtttact tttacctgtg tacctaaata agcatatact ggtactgtca ctttttgctt
    17761 tttgttgctt gccactgtgt aagtaatgtt attatctgca gctttatact tccctttttc
    17821 ccacgattct cctactttta cttcatgttt ttctaagtga ggcctttctt ttatcgcttc
    17881 tgttggggcg taatcgtata atcctcccat atctgatgtt gtataacgtg ggagtgtttc
    17941 ttcattgata acaatatgac cgtgttttga ttggataact tttgcgtaat tttcttttgc
    18001 tgctatagac gtgacgagta gaaacaccac agtagcgcca atgattaacc atgaatattt
    18061 tttattcata gtctttttca taaaaacaag cgttatgatc attgaagaaa aaaagatttg
    18121 gatacctaag atacgccaag gaaactggag taagcttatc ggtgtttttt gaaacaaaaa
    18181 ccatggaaat aaattagtta cgcataccca tgtagaaact acgccaacat agatgatttt
    18241 ttcttttttc tccatttctt tcagtatgaa taaaggaaaa atcaacgcaa ccaatcctaa
    18301 taaccccata gtgtatgttg taatatcgtt atttaacgct tgaatgatac tatctgaaat
    18361 agaaagtgct tctagctgca aatctgtttt gaagggactg tgtatttctt gataattcat
    18421 ctgctgcaac ataggatacc aaaaatagct agttaatgca caagtcaaaa aaacagcctt
    18481 tactaaattc acgaccacaa attttatatc gttttttatt tgcttgctta aaaacaaacg
    18541 aactataatc atacaagcaa tcaatgcact acacaatagt gtagttaaga catgagtata
    18601 cacaagtaga cttatactga cagataaagc gatgactttt ttttctctat tgaatatcaa
    18661 tccataagca taatataaaa cagctgggat aatcgtaaga gcgattgctt ctgcaattgc
    18721 ggatcgataa tagatatcaa ctgttcgata agttgcaaaa gtatataaac aactaaataa
    18781 aaaagcagca tctatctttt taaaaaactt ttctccataa taaaaactaa tcagaatcgt
    18841 acaaatattt aataaccaaa tatatagaac ataacttaat attaagttat ttgaaattcc
    18901 ataaaaaacg actgcaggaa ttattgtaag aaaaggataa aaatagttca gtccatctcc
    18961 ataattgtta aatgttgtat agttgattgg cccttcaaaa atagaagata accctttgat
    19021 tcgtgaaaga
//
LOCUS       DACDTO010000053        18740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDTO010000053 DACDTO010000000
VERSION     DACDTO010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.053"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            257..532
                     /locus_tag="IWK70_001833"
     CDS             257..532
                     /locus_tag="IWK70_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614358.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="IWK70_001834"
     CDS             545..2617
                     /locus_tag="IWK70_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9614359.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="IWK70_001835"
     CDS             2782..4668
                     /locus_tag="IWK70_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9614360.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="IWK70_001836"
     CDS             4680..5627
                     /locus_tag="IWK70_001836"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9614361.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="IWK70_001837"
     CDS             5647..6174
                     /locus_tag="IWK70_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9614362.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="IWK70_001838"
     CDS             complement(6237..6890)
                     /locus_tag="IWK70_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9614363.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="IWK70_001839"
     CDS             7022..7864
                     /locus_tag="IWK70_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9614364.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="IWK70_001840"
     CDS             8022..8918
                     /locus_tag="IWK70_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9614365.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="IWK70_001841"
     CDS             8921..9634
                     /locus_tag="IWK70_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9614366.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="IWK70_001842"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="IWK70_001842"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9614367.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="IWK70_001843"
     CDS             10169..10396
                     /locus_tag="IWK70_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9614368.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="IWK70_001844"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="IWK70_001844"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9614369.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11724)
                     /locus_tag="IWK70_001845"
     CDS             complement(11182..11724)
                     /locus_tag="IWK70_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9614370.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="IWK70_001846"
     CDS             complement(11961..12359)
                     /locus_tag="IWK70_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9614371.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="IWK70_001847"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="IWK70_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9614372.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="IWK70_001848"
     CDS             13472..14239
                     /locus_tag="IWK70_001848"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9614373.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="IWK70_001849"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="IWK70_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9614374.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="IWK70_001850"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="IWK70_001850"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9614375.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="IWK70_001851"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="IWK70_001851"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9614376.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3230 c         3899 g         5093 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agtccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt
//
LOCUS       DACDTO010000054        18724 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDTO010000054 DACDTO010000000
VERSION     DACDTO010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18724
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.054"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IWK70_001852"
     CDS             202..1077
                     /locus_tag="IWK70_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614377.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IWK70_001853"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IWK70_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IWK70_001854"
     CDS             complement(1642..2973)
                     /locus_tag="IWK70_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9614378.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IWK70_001855"
     CDS             complement(3122..3835)
                     /locus_tag="IWK70_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9614379.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IWK70_001856"
     CDS             4010..5434
                     /locus_tag="IWK70_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9614380.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IWK70_001857"
     CDS             5733..6440
                     /locus_tag="IWK70_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9614381.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IWK70_001858"
     CDS             6427..6750
                     /locus_tag="IWK70_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9614382.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IWK70_001859"
     CDS             complement(6839..7876)
                     /locus_tag="IWK70_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9614383.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IWK70_001860"
     CDS             complement(8197..9180)
                     /locus_tag="IWK70_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9614384.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="IWK70_001861"
     CDS             complement(9595..10365)
                     /locus_tag="IWK70_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9614385.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="IWK70_001862"
     CDS             complement(10358..11293)
                     /locus_tag="IWK70_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9614386.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="IWK70_001863"
     CDS             complement(11609..13258)
                     /locus_tag="IWK70_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9614387.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="IWK70_001864"
     CDS             complement(13375..15978)
                     /locus_tag="IWK70_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9614388.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="IWK70_001865"
     CDS             complement(15990..17039)
                     /locus_tag="IWK70_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9614389.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="IWK70_001866"
     CDS             complement(17065..17379)
                     /locus_tag="IWK70_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9614390.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="IWK70_001867"
     CDS             complement(17395..17841)
                     /locus_tag="IWK70_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9614391.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..>18724)
                     /locus_tag="IWK70_001868"
     CDS             complement(17842..>18724)
                     /locus_tag="IWK70_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9614392.1"
                     /translation="NIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYV
                     TLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDR
                     KVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFC
                     FFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDF
                     IKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANL
                     KTSSSFEKLVYILKEGL"
BASE COUNT         5736 a         3769 c         3069 g         6150 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 attt
//
LOCUS       DACDTO010000055        18225 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDTO010000055 DACDTO010000000
VERSION     DACDTO010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.055"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            128..1396
                     /gene="rseP"
                     /locus_tag="IWK70_001869"
     CDS             128..1396
                     /gene="rseP"
                     /locus_tag="IWK70_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9614393.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            1464..3173
                     /locus_tag="IWK70_001870"
     CDS             1464..3173
                     /locus_tag="IWK70_001870"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9614394.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            3385..7743
                     /locus_tag="IWK70_001871"
     CDS             3385..7743
                     /locus_tag="IWK70_001871"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9614395.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            7888..8361
                     /gene="rimP"
                     /locus_tag="IWK70_001872"
     CDS             7888..8361
                     /gene="rimP"
                     /locus_tag="IWK70_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9614396.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            8384..9559
                     /gene="nusA"
                     /locus_tag="IWK70_001873"
     CDS             8384..9559
                     /gene="nusA"
                     /locus_tag="IWK70_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9614397.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            9581..9874
                     /locus_tag="IWK70_001874"
     CDS             9581..9874
                     /locus_tag="IWK70_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9614398.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            9871..10182
                     /locus_tag="IWK70_001875"
     CDS             9871..10182
                     /locus_tag="IWK70_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9614399.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            10195..12501
                     /gene="infB"
                     /locus_tag="IWK70_001876"
     CDS             10195..12501
                     /gene="infB"
                     /locus_tag="IWK70_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9614400.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            12528..12875
                     /gene="rbfA"
                     /locus_tag="IWK70_001877"
     CDS             12528..12875
                     /gene="rbfA"
                     /locus_tag="IWK70_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9614401.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(13032..13205)
                     /locus_tag="IWK70_001878"
     CDS             complement(13032..13205)
                     /locus_tag="IWK70_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9614402.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            13653..16015
                     /locus_tag="IWK70_001879"
                     /pseudo
     CDS             13653..16015
                     /locus_tag="IWK70_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            16383..17306
                     /gene="truB"
                     /locus_tag="IWK70_001880"
     CDS             16383..17306
                     /gene="truB"
                     /locus_tag="IWK70_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9614403.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            17310..>18225
                     /gene="ribF"
                     /locus_tag="IWK70_001881"
     CDS             17310..>18225
                     /gene="ribF"
                     /locus_tag="IWK70_001881"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9614404.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQA"
BASE COUNT         6310 a         3236 c         3993 g         4686 t
ORIGIN      
        1 aaagacgcag gtttttattt ttcattatat tgatactatt ggtgtctttg gtatactatg
       61 gtaaaattat agtgttttat cgttgtttta taaaaaacac aaaaaacaag taaaggagaa
      121 aaaagctatg aaaacgattc tgacatttat catcgttttt ggtatattag tgattgttca
      181 tgagtttggt catttcttct ttgcgaaacg atcaggaatc ctcgttcgcg aatttgctat
      241 cggtatgggt ccgaaaattt atgggcatca agcaaaagac gggacaacat atacgcttcg
      301 cctattacct attggcggtt atgttcgaat ggcaggcaat ggggatgatg aaacagaaat
      361 ggcaccagga atgcctcttt ctcttttact aaattctgac ggcatcgtag agaagataaa
      421 tttaagtaag aaaatccaat tgacaaatgc tatcccgatg gaattgagcc gttatgattt
      481 agaagatgag ctgactatca ctggttatgt gaatggagac gaaacagaag tagtaaccta
      541 ccctgttgat cacgatgcga cgattattga aaacgacgga acagaaatca gaattgcgcc
      601 aaaagacgta cagttccaat cggctaaatt gtggcagcgt atgctgacca actttgctgg
      661 acccatgaat aactttattt tggcaatcct gttattcatc attctggcat ttatgcaagg
      721 tggtgttcaa gtgacgaaca ctaatcgcgt aggagagatc atgcctaatg gagctgcggc
      781 agaagcaggc ttaaaagaaa atgatgaggt agtcagtgtg gacggaaaag aaatccattc
      841 atggaatgat ctgacaacag tcattacgaa aaatccggat aaaacattag actttaagat
      901 cgaacgagaa ggtcaggtgc aatctgtcga cgtgacaccg aaatctgtag agtcaaatgg
      961 cgaaaaagtt ggccaacttg gtataaaagc cccaatgaat acaggtttta tggataagat
     1021 cattggaggg acgcgtcaag ctttcagcgg ttcattggaa attttcaaag ccttaggatc
     1081 actatttaca ggtttcagtt tagataaatt aggcggacct gtcatgatgt accaattgtc
     1141 ttcagaagca gcaaatcaag gtgtaacaac tgttattagt ttgatggcgc ttctttcaat
     1201 gaatctcgga atcgtcaatc tgcttccgat tcctgcctta gatggcggga aattagtctt
     1261 gaatattttt gaagggattc gcgggaaacc attaagtcaa gaaaaagaag gaatattgac
     1321 attagctggc tttggatttt taatgttgtt gatggtgcta gttacatgga acgatattca
     1381 acgattcttt ttctagaaaa agttgacaaa tagggtataa taacaaacga taactatttt
     1441 gaggaaataa aggagtatat agaatgagac aatcaaaaat gttgattcca acattacgcg
     1501 aagttccaaa tgatgcagaa gtattaagcc atcaaatctt gctaagagct ggatatatcc
     1561 gccaagtatc agcgggtatt tattcatatc tgccattagc aaatcgtgta ttggaaaaac
     1621 tgaaacgcat tatgcgggaa gaatttgaaa aaatcggagc ggtagaaatg ctgatgcctg
     1681 ccatcttgcc agcagaactt tgggaagaat ccgggcgtta cgaaacatat ggaccaaact
     1741 tgtatcgttt caaagaccgc aatgatcgaa aaatgatcct tggaccaact cacgaagaaa
     1801 cttttactga tttgatgcgt aatgaaatca attcatataa gaaattacct ttaaatcttt
     1861 atcaaatcca acctaaatat cgtgacgaaa aacgacctcg cttcggtttg cttcgcggaa
     1921 gagagtttat catgaaagat gcgtattctt tccatgcaac ggaagaaagt ttggacgaga
     1981 catataaaga ttacgaaaaa gcctacaccg aagtattcaa gcgttgtggc ttagaatttc
     2041 gctcgattat cggagacggt ggcgcaatgg gcgggaaaga ctcaaaagaa ttcatggcga
     2101 tctcagaaat cggggaggat acgatctgtt attcgactga aagtgattat gctgcaaact
     2161 tggagatggc tacaagctac tatgtatcaa aaaaatcgca tgaaacacag cttgaattgg
     2221 agaaagtagc tacacctaac gtaaaatcaa ttgacgaagt agcagatttc ttttctgttg
     2281 aacctcaaaa aatcatcaag tctgtattgt tcattgcgga tgaacaacct gtccttgttt
     2341 tggtacgcgg agaccatgaa gtcaatgacg taaaactgaa aaacttttta tctgctgact
     2401 ttttagaaga agcaacagaa gaagaagcac aaaaatatct aggtgctgag tttggttctg
     2461 tcggaccagt cggtgtttca gatgacgtaa aagtatatgc agaccgttat gtccaagatt
     2521 tagcgaatgc cattactgga gcgaacgaaa caggattcca ctataaaaat gtgaatccag
     2581 aacgcgattt cactgtactt tcttatgaag atcttcgttt tgtacaagaa ggagatcctt
     2641 caccagacaa caatggtgtt cttgccttta ctcgaggaat cgaaatcgga catatcttca
     2701 aaatcggcac tcgttacagt gaatctatgg gagcaactgt actagacgaa aatggacgag
     2761 aaaaatttgt gattatgggc tgttatggta tcggtgtaag tcgtttatta tcagcaatcg
     2821 tagaacaaca ttcagatgaa caaggaatca actggccaaa aggaattgcg ccatttgatt
     2881 tgcatatcgt ccaaatgaat ctaaaagacg aataccaaac aaacctgacg aatgaactag
     2941 aagaagcgat gacaaaagct ggctatgaag tactggttga tgacagaaat gaacgcgcag
     3001 gagtgaaatt cgcggattct gatttgattg gctgtccaat cagaatcact gtcggtaaaa
     3061 aagcagtgga tggaatcgta gaagtgaaaa tcaagaaaac tggcgaaatg gttgaagtac
     3121 gtaaagaaga attggtgaat actttaccaa tcctcttgaa ccaaggcgaa taataagcgt
     3181 taaaacacag aaaataaaaa tagctgaatc agctgttttt gacaatctga tgattcgctt
     3241 ttaaaattgg aactgcgatt tgaatcgcag ttccaatttt ttaatgtaaa actcagaaaa
     3301 aacgagtata ctaaaaggta ctgaaaaaga agaggataag atgcgatcaa ttgcattgtt
     3361 tctcatcaga aagggcgtat atctttgtca gaaaaacgag atttatttga aaaactgttg
     3421 gaacaaatcc aactagagga agcagaaaaa aatcatccac tgcttactgc tggtgaaatg
     3481 gatagtgtag tggtccatcg taaatcgcgt ttatgggaat ttacgctgca tttttccaaa
     3541 attttgccta ttatgctata tcggtcattg actcagcatc tgactatcgc ttttaaagat
     3601 attgcacaag tcaaactgaa tatcttagca gatgaccaga cattcgatga acagctcttg
     3661 caagattact gggcccaagc tttggaaaat cagcaatgcg atacaccact agcacagcaa
     3721 gtattgaaaa cgcaggtacc tgtgataaaa gatcgtaaag tcatcttgcc aatcgacagt
     3781 acaggagcta tttcttattt gaagcaacaa tacttgccat tagtagaaga gttgttcgtc
     3841 agttacggat tcccaaaatt tcgtatcgaa ccagaagtgg atgagcagca agcggaacga
     3901 gtattgaaac tttttgaaga acgcaaacaa gaacaggcag aagcctttat gaagcaagca
     3961 gctgaaagtc tagttgtcca cgaacaaaag aaaaaggaaa gaaaagagca atcgccagct
     4021 cttgaaggac ccatccacat ccagctagga cgaaatattc cagccgatga accaacgaca
     4081 ccaatgatca gtatcgttga agaagaacga agagtcacac ttgaggggta cgtattcgac
     4141 aaagaagtca gagaactacg ttcaaaacgc aagatcttga ctttgaaaat cacagattat
     4201 acttcatcat ttatcgtcaa aaaattctcg aacggcgaaa aagatgaaca agtatttgat
     4261 gcgatcagtg tcggtagctg gttaaaggta cgaggaagta ttcaagaaga tacgtttgtc
     4321 cgtgatttgg tcatgaatgc gcaagatatc atagaagtca aacatacacc tcgcaaagat
     4381 tatgctcctg aaggggaaaa acgagtagag cttcatgttc atagcaatat gagtacgatg
     4441 gatgcaacaa acagcatctc tgatcttgtg gcacaagcag ggaaatgggg acaccgagct
     4501 atcgccatta cagaccacgg tggtgcgcaa gcctttccgg aagcacatag tgctggaaaa
     4561 aaagcaggtg tgaaaatctt atacggcgtg gaagcgaatg tcgtagatga tggtgtccca
     4621 attgcttata atgatgcaca tgaagcattg agtgaagcca catatgtcgt atttgacgtg
     4681 gaaacgacag gattgtctgc agtatatgac acgatcatcg agctagccgc agtcaaaatg
     4741 tataaaggaa atgtgatcga aagctttgat gaatttatcg atccaggaca tcctttgtca
     4801 cgaacaacgg tagatctgac tggtatcacg gatggaatgg ttcgaggatc aaagtcggaa
     4861 gaagaagtat tgcggatgtt ccttgaattt tcaaaagata caattttggt agcccacaac
     4921 gcggcttttg atatggggtt cttgaatact agctacgcaa ggtatggtat tcctgaagcg
     4981 gccaatccag tcattgatac gttggaatta gctagatatc tgtatcctca attcaaacgt
     5041 ttcggattag gtgtgttatc taaaaaattt ggtgtcagct tggaacagca tcaccgagca
     5101 atctatgatg ctgaagcaac tggtcactta gcatggatct ttgtgaaaga ggcaatggac
     5161 aatcataaca tgctttatca tgatcagcta aatgagcata tcggagaagg agattcttac
     5221 aaaagggctc gacctttcca tgtaacgatt ttagcaaaaa atcaggcagg actgaaagat
     5281 ttgttcaaac tgatttcaat gtcgaatgtc gaatacttcg aacgcgtccc acgaatacca
     5341 cgctcccaat tgaaaaagat gcgtgaaaat ctattgatcg gctcagcttg tgataaggga
     5401 gaaatattcg aagccatgat gcaaaaaggg gtagaagagg caagaaaccg agcaaaattc
     5461 tacgattata ttgaagttat gccaaaagcg gtctatgcgc ctttgatcga gcaagaattg
     5521 gtaaaaaatg aacatgattt ggaagaaatt atccaaaatt tggtagaaat cggaaaaagt
     5581 ttggataaaa tcgttgttgc aacaggtaat gtacattatc tgaacgaaga agatgccatt
     5641 taccgaaaaa ttttgatcaa ttctatgggc ggagcgaatc cgttgaatcg tcatagtttg
     5701 ccggatgtcc atttccgaac aaccgatgag atgctaactg catttcactt tttaggggaa
     5761 gaaacagcga aagagatcgt agtagaaaat acgaataaaa tcgcagatat ctgcgaagaa
     5821 gtgattccag tcaaagatga actttataca ccgaagattc caggttcaga agatgaaata
     5881 tcagaattga gttacacaaa agcaaaacaa atgtacggag atccgttacc tgagatcatt
     5941 caaaaacgat taaaaaaaga gttgaattct attaatggta atggcttctc ggttatttat
     6001 ctgatcgccc aaaaactagt gcataaaagt aatgaagatg gctatctagt aggttcacgt
     6061 ggctctgttg gctctagctt tgttgcgacg atgacaggaa tcactgaagt taatccgtta
     6121 gcccctcatt attattgtcc agagtgccaa tattccgagt tttttgaaga tggtacgtat
     6181 ggttctggat ttgacatgcc agaaaaacaa tgtccaaaat gtggtgcacg cttaaacaaa
     6241 gacggtcatg atattccatt tgagacattt ttaggattcc atggagacaa agtgcccgat
     6301 attgatttga acttctcagg tgattatcaa gcagaagccc ataactatac gaaagtattg
     6361 tttggcgaag actatgttta ccgtgccgga acgattggta cagtcgccga caaaacagca
     6421 tatggctatg taaaaggata cgaaagggac aataatctgc agtttcgaag tgcagaagtc
     6481 gacagattag caaaaggcgc tactggagtc aaacggacaa ccggccagca tccagggggg
     6541 atcatcgtta ttcccgatta tatggatgtc tatgatttta cgccgatcca atatccagca
     6601 gacgatcaaa attcggaatg gaaaacgact cattttgatt ttcactcgat tcacgataat
     6661 gtattgaaat tagatattct agggcatgat gacccaactg ttattcggat gcttcaagat
     6721 ttatcaggga tcgatccaca aacgatccct acggatgacc cggaagtgat gcggatattt
     6781 gccggtcctg aagtacttgg agtaagtcag gaacaaattt attccaaaac agggacatta
     6841 ggtattccag aattcggcac tcgttttgtt cgagggatgc tagaagaaac gcatcctaca
     6901 acttttgcag aactactgca aatttccgga ctatctcatg gtacagacgt atggttagga
     6961 aatgcggaag aattgattcg acgaggagat gcgacactgg cggaagtaat cggctgtcgt
     7021 gatgatatta tggtttattt gatccatgct ggattagata gcgggatggc atttaaaatc
     7081 atggaaactg ttcgtaaagg acaatggaac aagatccctg atgaattgcg agaaacctat
     7141 ctatcggcga tgaaagaaaa taatgtgccc gactggtata tcgattcttg ttcaaaaatc
     7201 aaatatatgt ttccgaaagc ccatgctgca gcttacgtat tgatggctct tcgtgtcgct
     7261 tatttcaagg tttatttccc tattttgtat tactgtgcat acttttctgt acgtgcagac
     7321 gactttgatc tagtctccat gtgcaaagga aaagatgctg tgaaacaagc aatgaaagaa
     7381 atcacggaca aaggattaga tgcttccgta aaagaaaaga atcaactgac agtgctggag
     7441 ttagccaatg aaatgctgga acgaggattc aaatttggaa tgattgattt gtataaatcg
     7501 gatgccgtta actttgttat tgaaggtgat acgttgatcg caccatttag agctgtccca
     7561 agtttgggaa ccaacgtggc aaaacaaatc gttgaagcaa gaaaagacgg tccattttta
     7621 tccaaagaag atcttgccac ccgcggtaaa gtttcaaaaa cattgatcga atatatgaac
     7681 gacaatggcg tattgaaaga tctaccagat gaaaatcaat tatcgttatt cgatatgcta
     7741 taagcatctg ttttcctgtc tgcttgtaat tttaagcaga gtatggtata gttcttatag
     7801 gtaatgatac taactgtgag tgagcggatt tttcgctcac tctttttaat ggaattacgc
     7861 acttaatttt ttaaggaggc gaaacttttg agtagcgtcg ttgaaacagt cacagaaatg
     7921 gtgacaccga ttttagatga acaaaatttt gaacttgtcg aagtcgaatt tgtaaaagaa
     7981 ggaaaaagct ggttcttacg agtatttatt gacaaagaag gcggtatcga tatcgaagaa
     8041 tgcgcctttg tcagtgagaa actgagtgaa aaacttgatg cagcagatcc agatccgatt
     8101 cctcaagctt atttcctgga ggtatcctcc ccgggagcag agcggccatt gaaaaaagaa
     8161 agcgactatg aaaaggcact tggtgattat gtccatgtct ccttatacca accagtagat
     8221 ggcgaaaaac aatatgaagg atttcttcaa tcatttgatg ctgaacagct tacattgaaa
     8281 atacgcataa aaacacgaga aaaagaattg acttttgacc gaaaaaatat tgcaaaagct
     8341 cgcttagcta tccaatttta atcacggaga ggaatcagaa aaaatgagta aagaaatgtt
     8401 aaatgcgtta gacgctttag aagctgaaaa agggatctca aaagaaatcg tgatcgatgc
     8461 tttagaagca gcattggttt ctgcatacaa acgtcactat ggccaagcac aaaacgtcga
     8521 agtggaattt gatcagaaaa aaggaaagat ccatgtctat gcggtaaaag aagtaacgga
     8581 agaagtgatg gattcacagt tggaagtctc attgaaagat gcacttttaa tcaatccagc
     8641 atatgagatc ggtgatacga tccgttttga agtaacacca aaagattttg ggcggatcgc
     8701 tgcgcaaacg gctaaacaag ttattttaca gcgtgtacgt gaagcagaaa gaacgattat
     8761 ttataacgaa ttcagtgctt atgaaaaaga catcatgcaa ggaatcgtcg aacgccagga
     8821 taaacgttat atttatgtca atcttggtaa aattgaggca gttctttcta aacaagatca
     8881 aatgccaaat gaattttatc aaccacatga tcgaatcaaa gtttatgttt cccgagtaga
     8941 aaatacgtct aaaggtccgc aagtatttgt tagccgcagc catccagacc tattgcgccg
     9001 cttatttgag caagaagtac cagaagtcta tgatggaatc gttgaaattg tcagcatcgc
     9061 ccgtgaagct ggcgatcgtt caaaagtggc agttcgctca aatgatccaa atatcgatgc
     9121 agtcggtaca tgtgtcgggc caaagggaca acgagttcaa gcaatcgtca atgaattaaa
     9181 aggcgaaaac atggatatcg tcgaatggaa tgaagatcca gctgtattca ttgcaaatgc
     9241 attgaatcct gcacaagtca tggatgtgat cttcgatgag acaaatccaa aggcctgcac
     9301 agttgtcgtt ccagactacc aattatcgct agcaatcggt aaacgcggac aaaacgcacg
     9361 tttagctgct aaattgacta accataaaat cgatatcaaa tcagaatctg acatgacgga
     9421 attttatgaa aatcaagaac aacaaaaaga aacagaagaa ctgcatgatg aagcaatcgt
     9481 tcagtcagat ttaacagacg atgaatatga aacaattgca ttcaacaatg aaacggtcga
     9541 agaaaaacca gaagcttaac tttagtaagg aggcaaagag atgaaacaaa ggaaaattcc
     9601 tttacgcaaa tccgtggttt caggtgaaat gaaacctaaa aaagagatgg tccgaatcac
     9661 ccgttcaaaa gaaggggaag tatcgatcga tccaactgga aaaatgcctg gacgaggagc
     9721 gtatgtctca cttgagccgg aagaagtaca acaagcttgg gataaaaaaa ttctcgatcg
     9781 agtactcgaa acaacattaa cagatgagtt ttatcaagaa cttcttgact atgtcactca
     9841 ccaaaaagca cgtaaagagt tatttggaaa atgaatacag ttaatcggca aaaagccatg
     9901 aatctcatcg gtctagcgat gcgcgcggga aaactaatca cgggtgaaga actgaccatt
     9961 gctgaaatcc gcaaacaaaa agcaaaattg gtgtttgtgg caaccgatgc aagcgagaat
    10021 acaagaaaaa agataaaaga taagagttca tattatgaag ttccttgctt tgagctattt
    10081 tctgaagcag aaatcacgca gatgatcgga aaacctcgca aggtattcgg aataatggat
    10141 gccggatttg cgaagaagac caaggagcta attgaaggtt aggaaggtga ttgcatgggc
    10201 aacaaaagaa tctatgaatt agcaaaagaa ctcaaaaaat ctagtaaaga cgtcgtggaa
    10261 aaagcacaac aacttggcat cgatgtgaaa aaccatatgg gtgcgattac tagcaatgat
    10321 gagaagaaat tgcaacaagc gttcaaaaac ccaagcacga aacaacaagc tgcacaacaa
    10381 gcaagcagaa aaccagccaa tcaacagccg aacgatcaac gtaaaaaaga aacaacgaac
    10441 aatcaacaaa aaaataaaaa taaccgcagt tatcaagatc gcggtcaagg gagcggtcaa
    10501 gtgaatcaag gaaaaaaacc aacaacaaac caaaataata cgggaaatac cagcagccaa
    10561 aatcgtcaag gaacgagcca aggcggcaat caaaaccgta ctaacaataa caacaaacca
    10621 aacaataaca atcgcggcaa atttaataat aataaccgta atcgttataa taaaaaaggt
    10681 aaaaaaggca aacagcaaca atcaactaaa ccagctgtac caccacgcaa attccgtgaa
    10741 ttgccagatg tattagagta tactgaaggt atgaacgtag cagatattgc gaaaaaaatt
    10801 catcgtgagc cagcagaaat cattaaaaaa ttatttatgc ttggtgtcat ggtcaatcaa
    10861 aaccaagcac tggataaaga tacgattgaa ctgttagcga cggactatgg catggaacca
    10921 caagaaaaag tccaagttga cattgctgat atcgataaat tcttcgaggc agaagaagtc
    10981 gatccagata aattagtctc acgtccgcca gtcgtaacga ttatgggaca cgtagaccat
    11041 gggaaaacaa ctttattgga tacattacgc cattcacgtg taacaagcgg cgaagccggc
    11101 ggaatcacac agcatatcgg tgcctaccag atcgatattg atggtaaacc aatcactttc
    11161 ttggatacac caggacatgc ggcctttaca agtatgcgtg cacgtggagc aagtatcaca
    11221 gatatcacga tcttagttgt tgctgcagat gatggcgtca tgccacaaac agttgaagca
    11281 atcaaccatg ccaaagctgc aggcgttcca atcatcgtcg ctgtaaataa aattgataaa
    11341 ccaggtgcaa accctcaaca tgttatgcaa gaattaagtg agtatgagtt aattcctgaa
    11401 gcatggggtg gagaaacgat ctttgttgag atctcagcga aattcggcca aaacatcgaa
    11461 gaattgctgg aaatgatcct gttagtcgca gaagtagaag atttaaaagc agatccaaca
    11521 caacgcgcaa tcggaacagt gatcgaagca cgtcttgata aaggtaaagg tcctgtgtca
    11581 accttacttg tccaagaagg aacacttcat gttggtgacc caatcgttgt cggtaatact
    11641 tacggacgtg tccgtgtaat gacaaacgat cttggccgtc gcgataaaga agcaggacct
    11701 gcaacgccag tagaaatcac tggcttgaat gatgtacctc aagcaggtga ccgtttcgta
    11761 gtcttcgaag acgaaaagac agcacgtgca gcgggtgaag aacgcgcgaa acgtgcatta
    11821 ttagaaaacc gtgctgccac aagccgtgtg actttggata acttatttga aagcctgaaa
    11881 gaaggcgaat tgaaagaagt taacgtaatc atcaaagcag atgtacaagg ttcagcagaa
    11941 gcactggctg catcattgaa gaagattgat gtggaaggtg tccgtgtaaa aatcgtccat
    12001 tcagctgtcg gagcaatcaa tgaaagtgat gtaaccttag cagcagccag caacgctatt
    12061 atcatcggtt tcaacgttcg tcctacaccg caggcaaaag tgcaagctga tactgaagaa
    12121 gtcgatattc gtcttcaccg tatcatctat aaagccatcg aagaaatcga aactgcgatg
    12181 aaagggatgc tagaccctga atttgaagaa aaaatcaccg gtcaaatgat cgttcgtgaa
    12241 accttcaaag tttctaaagt tggtacgatt gcaggtgcct atgtaacaga aggatatatt
    12301 cgtagagata gcggtgtccg tgtaatccgt gacggaatcg taatctatga aggccaactt
    12361 gcaagtctga aacgattcaa agatgacgtg aaagaagtca aaatgggtta tgaatgtggt
    12421 gcaatgattg aaaaattcaa tgacatcaaa gtagatgacg taattgaagg atttatcatg
    12481 gaagaagtca aaaatgacta aaaactgata aaaggaggcc aatcattatg gctaactacc
    12541 gtgaccgccg agtcggtcaa gaaatcttaa aagaagtcaa tgatattttg cgcaaaaagg
    12601 tccgtgaccc tcgtgtggag aacgtaacga tcacagatgt gaaagttacg ggggacttac
    12661 agcaagcaac gatctattac agcattttat ctgacttagc atcagataag aaaacagcac
    12721 aagatggatt gaataaagca tcaggattga ttcgtcgaga gttagggcac aatctaagta
    12781 tttataagac acctgaattg ttctttgaac ttgatgaatc agttgtctat ggcaaccata
    12841 tcgatgaatt gcttcgcgac ttgaacaaag actaatcaaa aactcccgca aatcctgtga
    12901 aatcaggggt tgcgggattt ttttgttttc attaagacgt attacctaag agttccatga
    12961 ctttagtctt gcggcgtgtc agctaaccaa aaaagagaaa aagaaaagta aaaaattcga
    13021 ctttcttttt tctagtagta tataaatttt tgcatataga taaagatcaa gataccgata
    13081 acggaagcta gattgtaatc tattttagtg acagtcaggt atagccaagt aactagaaga
    13141 tcagtggaac ctgcaccata aaagtttgag tttgtcggtc cgttgacagt taaaagaaaa
    13201 atcacgttga agttattgat atttccgatt acgccgcttc gatttggtca cgatccaaat
    13261 tttggataat ccctgtagaa attagcatag tagctgggat accaattcac atattgacaa
    13321 agataacaga gacttttaca taaaacggat caatcaggaa aggaattgct cgatccagcc
    13381 ccagttttcc agcaatgtgt taactggccc agcgtcattc agcaaattat gcatgatgag
    13441 gagcgaaatg aactgcggaa cagccattgt gataacgaca agttttttgg atcaatgtaa
    13501 aaaacgtcag cgaaaggaaa aatttcgtgg ttttttatac ctgttcattt ggctagatat
    13561 aaaatccaaa caaatgaaca gaagtgctta ttaggtttca attgggagta tctgcgctag
    13621 aataagagta ataatgaaaa ggagtgttat gtatggatta ttctagtaaa gaatactttg
    13681 ataaaatgac ggcttggtgg cgtgctgcta attatctttc tgttgggcaa ctttatctta
    13741 aagataaccc cttactgaga cgtacactga aacccgaaga tgtcaaaaaa catccaatcg
    13801 gtcattgggg aacgattcct ggccaaaatt ttatttatgt ccatttgaac cgagtcatca
    13861 ataaatatga tttgaacatg ttttatattg aagggccagg acacggcgga caagtcatgg
    13921 tatcgaatgc ttatttagat ggaagttaca cagagatcta tccagaagta acggaagatg
    13981 agacaggaat gcaaaaatta ttcaaacgat tttcatttcc tggaggcatt gcttctcatg
    14041 cagcaccaga aacaccaggt tctatccatg aaggcggaga gcttggatac tctctttcac
    14101 atgcagtcgg agctgtatta gacaatcctg aagtaatctc tgctgtcgtg attggtgatg
    14161 gtgaagcaga aaccggtcct ttagctggaa gctggttctc aaatgtcttt atcaatccag
    14221 tgatagatgg agctgtttta ccgatccttc atttaaatgg tgcaaaaata gccaacccaa
    14281 cgatcttggc acgtaaatca gatggagaat tagcaaatta ctttaacgga cttggctggg
    14341 aacctttctt tattgaagga aatgattcag aaaaattaaa tcctgttatg gctgaaaaaa
    14401 tggatcaagc aatcgagaaa attaaatcca tccaaaaaga agctcgcctg aagacagcaa
    14461 cagatgtcgt gatgccaaaa tggccagtat tgatcgtacg cacaccaaaa ggatggaccg
    14521 gtccaaaaga atgggacggg gaaccaattg aaggaacctt ccgagcacac caagtaccga
    14581 ttcctgtaga tcaagaacat atggatcatg cagatgcttt actccgttgg ctgaaatcct
    14641 atgaaccaga aaaattattt gatgcacaag gacgtatttt agaagaaatc agagaaatcg
    14701 cgcctactgg tgaccaaaga atggcaaaaa atcctatcac caatggtgga atcgatccaa
    14761 gaccgcttat tatgccagat tggaaaaaat atactttgca atttgaaaaa ccaggctccg
    14821 ttaaagcaga agatatgacc gaacttggta aattcgttcg cgagatcatt gagaaaaatc
    14881 cagaaaattt ccgtatattt ggaccagatg aaacaaaatc aaaccgattg aatcaagttt
    14941 tcaaaacgac aaatcgccaa tggatggaaa aaatcgaacc agaaaatgat gaatggcttt
    15001 cgccatctgg tcgcgtcatc gacagtcaat tatcggaaca ccaagatgaa gggttcttag
    15061 aagggtacgt tctgactgga cggcatggtt ttttgcaagt tacgaatcat tcttgcgagt
    15121 ggttgactct atgttgactc agcacttcaa atggatgcgg aaatcacatg acctttcttg
    15181 gcgaaatgat tatccttcgc taaatctcat tgcaagctca acagtcttcc agcaagatca
    15241 caatggatat agccaccaag atccaggaat attgacacat ttggcagaaa agaaggcgga
    15301 gttcatccgt gaatatctgc ctgcggatgc aaatacattg ctcgcagtca tggataaagc
    15361 attccgctcg tcagaaaaaa tcaatttgat catctcttcc aaacatccgc gtgctcaatt
    15421 ttactcagca gaagaagcag ccgtcttagt gaatgaaggc ttgaaaatca ttgattgggc
    15481 ttcgacagca aaagaggagg aacctgaatt ggtcattgct gctgcaggaa cggaatctaa
    15541 cttagaagca ttagcagctg taactttgct gctggaagaa ttcccaaaac taaaaattcg
    15601 gttcatcaat gtcgtagatt tattgaaatt acgtcatcca agtcaagatc cgcgtgggtt
    15661 gagcgatgaa gaatttgatc aatattttac aaaagacaaa ccgatcttat ttgctttcca
    15721 cggttatgaa acattggtcc gcacgatttt ctttgatcgt cataaccatc atttaatgat
    15781 tcatggctac aaagaaaatg gagatatcac gacaccattt gacatgcgtg tagtaaatga
    15841 acttgatcgt tatcatttgg caaaagacgc agctttaaaa atcaaaggaa gtcaagcaga
    15901 agattttgcg aaaaaaatgg atcagaaatt acaagaacat caaaactata ttcgtgaaaa
    15961 tggtattgat ctgccagaag tattggattg gaaatggaaa aatcttgacc aataaaatga
    16021 taaaatcgat cccgcaaaat agaatttttt acattgatta agaaactgaa aaaataaata
    16081 aaaaaaacgg aaaaaagact ggaagaatgg ccagtctttt ttttcgtttt tgggaagtga
    16141 aaaccaaact atttacctgt aagccaccca taaatccaat caaatggtgc aaagaaaaaa
    16201 tcttccattc tattccctcc tttaaaaaag cataacatga tttctattta cgtgagcatg
    16261 aattttgtta cgtttttatt gccgaaaaat aaaaaccgtt cattatgatg tcaaattcgt
    16321 cagtaattga tgcaatgtgt tataataatt cagttaaatc aaaaatgaaa tggagaaaag
    16381 acatggacgg gttattacct ttatggaaag aacgcggaat gacaagtcat gattgcgtat
    16441 tcaaattaag aaaaatctta catacgaaaa aaatcggaca tgggggtacg cttgacccag
    16501 acgtagacgg tgtgttgccc atttgtattg gaaaagccac aaaagttatc gaatatttga
    16561 cagattctgg gaaaatctat gtaggcgaga taactctagg tttcagtaca acgacggaag
    16621 atcgatccgg agaacgggta gaaacaaaac cagtagatat gtctttatct gaaaaaaaaa
    16681 tagatgaagt gatggcttca tttattggtg agatcaccca gataccacca atgtactcag
    16741 cggtaaaagt caacggtaag cggttatatg aatatgcccg caataatgaa acagtggagc
    16801 gtcctgttag aaaggctcag atttaccgtt ttatccggac aagtgaattg acaagagacg
    16861 aagtgacagg aaccatttct tggcgcttcg aagtagaatg cgggaaagga acctatgtcc
    16921 gcacattagc tgtagataca ggcaagaaac ttggttaccc tgcgcacatg tctgatctga
    16981 cccgaacagc aagtgccggt atgtcccaga aggatgctct gacattagaa gaagtagctc
    17041 aagctgtgaa aaatgggaca atcgatgatt gtttgctgcc aatagaaaaa ggagtggaac
    17101 ggttcaaacg agtagatatt actgatgaag tatggcagaa agtaaaaaac ggtatgagac
    17161 ttgattatac tgtatttgcg ctcccagaaa tgccgtcaga agaaattgct ttgttttata
    17221 aaggaaaagt tgtcagtatt tatcagccaa acccgaaaga aaaaaacaaa ttaaaaccta
    17281 gtaaagtatt gagaaatgag gaataacgaa tgaatatcat caagatcaga catccttacc
    17341 aatcagaaca aattccagaa gacgatgtcg ttttagtgct gggatttttc gacggcgtgc
    17401 atatcggaca ccagaaagtc ataaagacag gtatggaaat tgcacaaaaa gaaggattga
    17461 aattagcatt gatgactttc aatcagcatc catctattgt ctttaaaaag atcgatccta
    17521 gcagcgtgaa atatttgact agccttaagc aaaaagaaga aaaaatggcc gctttgggta
    17581 tagattattt atatgaagtt gattttactt cttcttttgc tcatttagca ccgcaggaat
    17641 ttgttgatca atacatcgta gggttacatg caaaatatgc tgtttccggt tttgattata
    17701 cgtatggacc aaaagacatt gcagatgtcg ctcattttcc tgaatatgcc aaaggaagat
    17761 tcaaaatcgt cacagtttct aaagaagaag aacaaggtga aaaaatcagc tctacccgta
    17821 ttcgtggatt actagacagc ggacaagtaa aagaagcagc aaaactatta ggttgcacat
    17881 atgaggtaga aggggtagtt gtccatggcg atgcacgagg cagactacta ggattcccta
    17941 cagcaaatgt caaagtaaag agcacggttc atttgcctaa agaaggtgtt tatgtgtcgg
    18001 aaataaaaat tggggaaaca tggtatccag ccatgggttc aattggacat aatgatacat
    18061 tcggtgaagg acgggagctg actgtagagt tgtatatttt ggactttaat caagatatct
    18121 atggtgaaca tgtcgcggta cgctggaatc aatttttaag agatcaagtg aaattttcta
    18181 gtgtagagga attgattgaa caactcaagg aggatgaaca agcaa
//
LOCUS       DACDTO010000056        18080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDTO010000056 DACDTO010000000
VERSION     DACDTO010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.056"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(9..1487)
                     /locus_tag="IWK70_001882"
     CDS             complement(9..1487)
                     /locus_tag="IWK70_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9614405.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(1785..2720)
                     /gene="murB"
                     /locus_tag="IWK70_001883"
     CDS             complement(1785..2720)
                     /gene="murB"
                     /locus_tag="IWK70_001883"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9614406.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(2793..3713)
                     /locus_tag="IWK70_001884"
     CDS             complement(2793..3713)
                     /locus_tag="IWK70_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9614407.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            3826..4578
                     /gene="xth"
                     /locus_tag="IWK70_001885"
     CDS             3826..4578
                     /gene="xth"
                     /locus_tag="IWK70_001885"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9614408.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            5442..5975
                     /locus_tag="IWK70_001886"
     CDS             5442..5975
                     /locus_tag="IWK70_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9614409.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(6465..6989)
                     /locus_tag="IWK70_001887"
     CDS             complement(6465..6989)
                     /locus_tag="IWK70_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9614410.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(6982..7455)
                     /gene="tsaE"
                     /locus_tag="IWK70_001888"
     CDS             complement(6982..7455)
                     /gene="tsaE"
                     /locus_tag="IWK70_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9614411.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(7578..8558)
                     /gene="pta"
                     /locus_tag="IWK70_001889"
     CDS             complement(7578..8558)
                     /gene="pta"
                     /locus_tag="IWK70_001889"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9614412.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(8711..9391)
                     /locus_tag="IWK70_001890"
     CDS             complement(8711..9391)
                     /locus_tag="IWK70_001890"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9614413.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            9517..10386
                     /locus_tag="IWK70_001891"
     CDS             9517..10386
                     /locus_tag="IWK70_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9614414.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            10412..10927
                     /locus_tag="IWK70_001892"
     CDS             10412..10927
                     /locus_tag="IWK70_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614415.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(10974..11537)
                     /locus_tag="IWK70_001893"
     CDS             complement(10974..11537)
                     /locus_tag="IWK70_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9614416.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(11592..11771)
                     /locus_tag="IWK70_001894"
     CDS             complement(11592..11771)
                     /locus_tag="IWK70_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614417.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(11848..12357)
                     /locus_tag="IWK70_001895"
     CDS             complement(11848..12357)
                     /locus_tag="IWK70_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614418.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(12453..12833)
                     /locus_tag="IWK70_001896"
     CDS             complement(12453..12833)
                     /locus_tag="IWK70_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9614419.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            13163..13912
                     /locus_tag="IWK70_001897"
     CDS             13163..13912
                     /locus_tag="IWK70_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9614420.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(14096..15373)
                     /locus_tag="IWK70_001898"
     CDS             complement(14096..15373)
                     /locus_tag="IWK70_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614421.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(15452..16096)
                     /gene="pcp"
                     /locus_tag="IWK70_001899"
     CDS             complement(15452..16096)
                     /gene="pcp"
                     /locus_tag="IWK70_001899"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9614422.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(16295..17239)
                     /locus_tag="IWK70_001900"
     CDS             complement(16295..17239)
                     /locus_tag="IWK70_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9614423.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(17241..17927)
                     /locus_tag="IWK70_001901"
     CDS             complement(17241..17927)
                     /locus_tag="IWK70_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9614424.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         5262 a         3688 c         3121 g         6009 t
ORIGIN      
        1 cataaatact agctgatttt ttcagttaaa tgtctatttt tttctctgat gatggtattt
       61 ccaaaatatt cccagtcatc tggagtcggt ggatttttcc aaattatctg ttctttatta
      121 aatttttcaa gtaaacaatc agataaataa atattcgtac gagaagtgat tcgactctca
      181 atcacaatat tgtaattttt aaatcctttc acttgagaaa taatgtaatc ggtataagca
      241 gatccttgcg aaaaatcaat acagacatga atcggtgctt caaaatatga taccggacaa
      301 ataccgacca ataaaaagag ataatcataa aataagcgaa tcatgaaacg ttcatctttt
      361 cccaagaaat agcgtttatt ctcttgtatg accttttgga ttgctgtact gaatattgga
      421 tacgtctcga agaaaaactg tagctgcttt ttagacgtaa agctagtcat ctcaaagtcg
      481 aatatccaat tttttcgatt gacatgcatc atacccatac gaagttcttt tcgtatatat
      541 agtctttctt cttcagagat ttcaatcgaa aatggtaatt gatcaatcaa ttccactgac
      601 attttttgac taatcatgtg gatatcatga aaatgagctt ccgcaagtcc aaatgcgcca
      661 tctaattcaa attcttcttc cgtgatcaaa aacaacatga tataagaaat ctcatcaatg
      721 atctgctctt tagggatatg aagaaaagaa atatgtgata aatccttgat gatcttcatt
      781 ccttctgagc tcttaaagta ttcatctgaa aagaaacgtt ttccgattgg ttgattccca
      841 taggccctca acaaactaat agtggttagc aaattgaatt tgattcgttt tgttgcagac
      901 agttttaaat tgaacagata ttcaaaatat tgcacgatac tctttgcttc attcatgacg
      961 ctttctctaa atggcataga aaaaccatta aagaccccga aatatacccc aaataatacg
     1021 ttccgtatgt ttatttcttc cccaactaat atgtttttct ttaactttat ttgatgctgt
     1081 tcaagaaaag agactaattc tcttcttttt acataggctc ttgatatgct taaaaaatga
     1141 ttctctgcaa atttttcgat cgttactttt gaatccacga tctccgttaa cagtcgagca
     1201 atttttgatc gttgaaagta gattcttctt aaatttttga tatccgtcaa attcagacgt
     1261 tcgacatgga tctctgcttc ttctgaaatt tggatctctg gctttgtttt gaattcagta
     1321 aattctttat ttaattctat gatataattt cttgttttaa atttagacaa acctaatgtt
     1381 tcatataact taggcagcac tacataattc tttttctcaa attctaaata tctagctagc
     1441 agcagttttt ctctatcttc ctcttctata aggtaatcca acttcatatc ctacacctca
     1501 aaaaatgcct cataccaatt acttgatatg cagatgaaag ttcatctttt cacctatact
     1561 gattttatga tggattatgc ttgttttttc atcagttatt ctcatttttt atcttttttt
     1621 tgagaatatt gcaaaaaatg gaaaaattta tgaggaaata ttctatattt tttgttttat
     1681 tgtgttaaaa tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac
     1741 ggccaccgtc ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt
     1801 ctgcttgttt ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac
     1861 acatcaaatt tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc
     1921 gctgtcgcat gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg
     1981 ccccatttca ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact
     2041 gggcgcttaa agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat
     2101 tcggtcaact catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat
     2161 cgagcttcca atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc
     2221 atttcgtctt ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca
     2281 aaaacatctt tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca
     2341 cttcccggga tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa
     2401 gtcgtatcaa tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc
     2461 tgtttcattt cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga
     2521 ttgcttgcat ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt
     2581 tgctttactt cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc
     2641 gtaaacgtat agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca
     2701 actatttctt ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc
     2761 tatcacgctt aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta
     2821 agcacttcct cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt
     2881 tccgatttac ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt
     2941 ttgactagcg tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcctcttga
     3001 atgacaagct gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat
     3061 ttttctatat tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt
     3121 tctaattgta aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt
     3181 ctcaaaatgg gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca
     3241 taaaggacga actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac
     3301 cgcttatctg ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg
     3361 atcatcaaaa ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga
     3421 tggacactca gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct
     3481 aattcataat gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa
     3541 agctcatcga tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt
     3601 gtttctttat ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt
     3661 ggtaaatatg ctttttctgc gatattccct acaccgatta caccaatctt catctacttt
     3721 cctcctggat catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat
     3781 agtatcgcaa aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct
     3841 tggaacgtaa acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag
     3901 ttagacgccg attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta
     3961 gatcttccag gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca
     4021 gcgatttttg ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat
     4081 gatacagaag gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac
     4141 acgccaaatt ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc
     4201 ttttataatt atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta
     4261 aatgtcgctc atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga
     4321 ttcactccag aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca
     4381 ttccgttatt tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat
     4441 tcaaggaaaa acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag
     4501 ccaggactag ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta
     4561 gaattagatt tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa
     4621 attttgtgaa aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc
     4681 ttttgaacac tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac
     4741 agccaaacaa aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca
     4801 agcttttgaa caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag
     4861 aacagccaaa caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat
     4921 gcaagctttt gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat
     4981 aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttggact tattaccgca
     5041 gatgcaagct tttgaacacc gtttattgag aagttgtgaa atcagcgtcc tgacctgagt
     5101 attagagaaa caaatggcaa aatagttcct catattttgc tattttttga gctaataccg
     5161 agggtcagct gattgaacac cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga
     5221 gtcgtaagaa atttttttga aaatggttcc tcacaacttt gcataaattt gacttaatac
     5281 cgagaagtca tgatccccat ctaactccaa atcacaagga atgagttcaa aaactagctt
     5341 ttctcaacaa atattacata aaaaattcta tgttttttgt taaatttatt tgcttttttc
     5401 aaaagtatgt tacagtaatt tcccaaggag gttttacaga aatgaatttt gttgccatgg
     5461 attttgaaac tgccaatcat cagccttata gtgcttgttc tcttgcgcta gtcatggtaa
     5521 aaaatagcca gatcgttgat gaattctata cattgataca gcctgagact ccttttttct
     5581 ggcgaaatgt ccaaattcac ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc
     5641 cagaagtctg gcaaactatc caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata
     5701 atgctgcttt tgatacaaag gtcttagcgg gatgcttaga ttattatcat cttgaacagc
     5761 ctaattatct ttctttatgt acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa
     5821 atcatcgatt gaacactgtg tgcgaaaatt tgaacatcca attagatcat caccatgatg
     5881 cattggaaga tagtcgtgca tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga
     5941 cagatccatt gaaaaaactt gtgactataa agtaaaagaa tacttatgaa acacacaaag
     6001 aggtcgtgag cctgccgttt cactccgagt cacaaggagc aatttcaaaa aatagcttct
     6061 catgtttatt aaatattgtg actggtgacc gaggagctgg caggagaaca ccgtttattg
     6121 gagaggttgt gaaaaagctg ttctgcatca agtaataaga acagccaaac aaaaatagct
     6181 tctcatattt ttcatttggt tggacttatt accgcagatg caagcttttg gacaccgttt
     6241 accaagaggt tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggcaaaata
     6301 gttcctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
     6361 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
     6421 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
     6481 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
     6541 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
     6601 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
     6661 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
     6721 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
     6781 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
     6841 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
     6901 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
     6961 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
     7021 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
     7081 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
     7141 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
     7201 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
     7261 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
     7321 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     7381 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     7441 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     7501 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     7561 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     7621 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     7681 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     7741 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     7801 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     7861 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     7921 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     7981 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     8041 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     8101 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     8161 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     8221 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     8281 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     8341 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     8401 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     8461 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     8521 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     8581 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     8641 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     8701 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     8761 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     8821 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     8881 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     8941 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     9001 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     9061 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     9121 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     9181 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     9241 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     9301 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     9361 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     9421 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     9481 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     9541 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     9601 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     9661 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     9721 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     9781 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     9841 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     9901 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     9961 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
    10021 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
    10081 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
    10141 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
    10201 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
    10261 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
    10321 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
    10381 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
    10441 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
    10501 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
    10561 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
    10621 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
    10681 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
    10741 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
    10801 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
    10861 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
    10921 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
    10981 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
    11041 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
    11101 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
    11161 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
    11221 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
    11281 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
    11341 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
    11401 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
    11461 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
    11521 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
    11581 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
    11641 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
    11701 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
    11761 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
    11821 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
    11881 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
    11941 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
    12001 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
    12061 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
    12121 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
    12181 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
    12241 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
    12301 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
    12361 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
    12421 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
    12481 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
    12541 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
    12601 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
    12661 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
    12721 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
    12781 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
    12841 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
    12901 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
    12961 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
    13021 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
    13081 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
    13141 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
    13201 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
    13261 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
    13321 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
    13381 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
    13441 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
    13501 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
    13561 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
    13621 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
    13681 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
    13741 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
    13801 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
    13861 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
    13921 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
    13981 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
    14041 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
    14101 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
    14161 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
    14221 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
    14281 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
    14341 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
    14401 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
    14461 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
    14521 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
    14581 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
    14641 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
    14701 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
    14761 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
    14821 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
    14881 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
    14941 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
    15001 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
    15061 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
    15121 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
    15181 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
    15241 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
    15301 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
    15361 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
    15421 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
    15481 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
    15541 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
    15601 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
    15661 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
    15721 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
    15781 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
    15841 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
    15901 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
    15961 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
    16021 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
    16081 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
    16141 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
    16201 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
    16261 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
    16321 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    16381 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    16441 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    16501 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    16561 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    16621 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    16681 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    16741 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    16801 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    16861 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    16921 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    16981 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    17041 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    17101 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    17161 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    17221 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    17281 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    17341 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    17401 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    17461 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    17521 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    17581 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    17641 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    17701 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    17761 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    17821 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    17881 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    17941 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    18001 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    18061 acttattgta aggtaaagct
//
LOCUS       DACDTO010000057        17979 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDTO010000057 DACDTO010000000
VERSION     DACDTO010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.057"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IWK70_001902"
     CDS             complement(27..725)
                     /locus_tag="IWK70_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9614425.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IWK70_001903"
     CDS             complement(718..2070)
                     /locus_tag="IWK70_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9614426.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IWK70_001904"
     CDS             complement(2293..3693)
                     /locus_tag="IWK70_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9614427.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IWK70_001905"
     CDS             3854..4591
                     /locus_tag="IWK70_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9614428.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IWK70_001906"
     CDS             4716..5195
                     /locus_tag="IWK70_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9614429.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IWK70_001907"
     CDS             5192..5959
                     /locus_tag="IWK70_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614430.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IWK70_001908"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IWK70_001908"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9614431.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IWK70_001909"
     CDS             complement(6862..7464)
                     /locus_tag="IWK70_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9614432.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IWK70_001910"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IWK70_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IWK70_001911"
     CDS             8145..8729
                     /locus_tag="IWK70_001911"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9614433.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IWK70_001912"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IWK70_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9614434.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IWK70_001913"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IWK70_001913"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9614435.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IWK70_001914"
     CDS             10630..10809
                     /locus_tag="IWK70_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614436.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IWK70_001915"
     CDS             10831..11856
                     /locus_tag="IWK70_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9614437.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IWK70_001916"
     CDS             12031..13275
                     /locus_tag="IWK70_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9614438.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IWK70_001917"
     CDS             13428..14375
                     /locus_tag="IWK70_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9614439.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IWK70_001918"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IWK70_001918"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9614440.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IWK70_001919"
     CDS             complement(15277..15519)
                     /locus_tag="IWK70_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614441.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IWK70_001920"
     tRNA            15640..15712
                     /locus_tag="IWK70_001920"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IWK70_001921"
     CDS             complement(15831..16469)
                     /locus_tag="IWK70_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9614442.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IWK70_001922"
     CDS             complement(16592..17224)
                     /locus_tag="IWK70_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9614443.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IWK70_001923"
     CDS             17575..17874
                     /locus_tag="IWK70_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9614444.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6157 a         3150 c         3555 g         5117 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaag
//
LOCUS       DACDTO010000058        17344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDTO010000058 DACDTO010000000
VERSION     DACDTO010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.058"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..368
                     /locus_tag="IWK70_001924"
                     /pseudo
     CDS             1..368
                     /locus_tag="IWK70_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            548..1489
                     /locus_tag="IWK70_001925"
     CDS             548..1489
                     /locus_tag="IWK70_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9614445.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1558..2289
                     /locus_tag="IWK70_001926"
     CDS             1558..2289
                     /locus_tag="IWK70_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9614446.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2433..4613
                     /locus_tag="IWK70_001927"
     CDS             2433..4613
                     /locus_tag="IWK70_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9614447.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4633..5115
                     /locus_tag="IWK70_001928"
     CDS             4633..5115
                     /locus_tag="IWK70_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9614448.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5131..5892
                     /locus_tag="IWK70_001929"
     CDS             5131..5892
                     /locus_tag="IWK70_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9614449.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6023..7357
                     /locus_tag="IWK70_001930"
     CDS             6023..7357
                     /locus_tag="IWK70_001930"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9614450.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7785..8270
                     /locus_tag="IWK70_001931"
     CDS             7785..8270
                     /locus_tag="IWK70_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9614451.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8263..8946
                     /locus_tag="IWK70_001932"
     CDS             8263..8946
                     /locus_tag="IWK70_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9614452.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9072..11717
                     /gene="polA"
                     /locus_tag="IWK70_001933"
     CDS             9072..11717
                     /gene="polA"
                     /locus_tag="IWK70_001933"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9614453.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELADKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11762..12598
                     /gene="mutM"
                     /locus_tag="IWK70_001934"
     CDS             11762..12598
                     /gene="mutM"
                     /locus_tag="IWK70_001934"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9614454.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12562..13191
                     /locus_tag="IWK70_001935"
     CDS             12562..13191
                     /locus_tag="IWK70_001935"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9614455.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13243..13575
                     /locus_tag="IWK70_001936"
     CDS             13243..13575
                     /locus_tag="IWK70_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9614456.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHISDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13887..14378
                     /gene="nrdR"
                     /locus_tag="IWK70_001937"
     CDS             13887..14378
                     /gene="nrdR"
                     /locus_tag="IWK70_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9614457.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14401..15774
                     /locus_tag="IWK70_001938"
     CDS             14401..15774
                     /locus_tag="IWK70_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9614458.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15774..16700
                     /gene="dnaI"
                     /locus_tag="IWK70_001939"
     CDS             15774..16700
                     /gene="dnaI"
                     /locus_tag="IWK70_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9614459.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16829..17269
                     /locus_tag="IWK70_001940"
     CDS             16829..17269
                     /locus_tag="IWK70_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9614460.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5908 a         3032 c         3820 g         4584 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tcggaaaaag atcgtcaaga aatggatggg acgcttataa
      121 ttgaagtagg agttgaggac agtcaaacct catttctgtt atatgatcga gcatttgttg
      181 agtcccattc acctggcaca gctactagtg ccccatcttt gatgttttac agtaaagata
      241 tctttggact atataaaaaa atgcagacgc aatcagtcac attaggtgat atggtacagc
      301 ttggagaaga atatgtcttt aactttgctg atcccgatgg caactacttt gcggttacag
      361 gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa caggaaagcg aagtcttttt
      421 aattcaaata ataatattat gtaaacaaaa aactttccat gcgtaattat acacaagcaa
      481 ataaatcgtc aaatgcgaac gccttcttta tactgtttag gaacgaaaag aaggaaggaa
      541 gaaatacatg gattatcaag tactgcttta ttacaattat acaaaaatcg aagaaccaga
      601 ggaatttgcc aaaaatcatt tagccttttg caaatctttg gatcttaaag gtcgtattct
      661 agtagctgaa gaaggaataa atggtactgt gtctggaacc gttgaaaaca cgactgccta
      721 tatgaaagca atgaaagctg atcctcgatt tgctgatacg atgttcaaga tcgatccaag
      781 cgaaggacat gcctttaaga agatgtttgt aagataccgt ccagagcttg taagtttaaa
      841 gctagaagag gacattgatc caaaccaagt gacaggtgcc tatctttcac cgcaagaatt
      901 taaagaagct attttagatg aacagacagt ggtgattgat gcacggaatg attatgaata
      961 tgacttggga cattttcgag gggctgtacg cccagatata cgcagttttc gagagcttcc
     1021 agactggatc cgcgagcaca aagaagaatt tatggaaaaa cgggtagtga cctactgtac
     1081 tggtggcatt cgttgcgaga agttttctgg ttggcttgtc cgtgaaggat tcaaagatgt
     1141 cggacagctg catggcggta ttgctactta tggtaaggat cctgaagtcc aaggagaatt
     1201 atgggacggc aagatgtatg tgtttgatga acggatcgct gtcgatatca accaagtaga
     1261 taaacaaatc gtcggtcgcg actggtttga cggaacacca tgtgaacgat atatcaattg
     1321 tagcaatcct gagtgcaatc gtcagattct tgcttccatt gaaaatgaag aaaaacattt
     1381 aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat cgctatgttc aagaccatgg
     1441 aatctctcca gaagaagcag aactgcggtt tacagcttta attaaataga aaacaatagt
     1501 tttgattgtc gaccatttcc aatatgcgct atactagcgt tgaggatggt gatccacatg
     1561 aaatcattga tttatgctat tggggatgtc catggcaaac atgagctttt tgaaaaactt
     1621 attcactatt atgatccaaa gatccatcag cttgttttat taggggatct caatgatcga
     1681 ggtcctcgta caaaagaatg ttttttatta ggaaagcaat tagtcgaaga aacaggagcc
     1741 atttacctcc ggggaaacca tgaagaatat tttttgcaat ttctgcaatc accggaggac
     1801 tggtatgcgc cttatatgag aaatggtgga aaagaaacgt ttgaaagcct gattcaccca
     1861 ggggcatcgg aggaatactc cccaactgaa atctcgatga tgatccaatc gagatattca
     1921 gaactgatta aatttttagt ccagcgcccc ttgtattatg aatgggaaaa ttatcttttt
     1981 gtccatgccg gtgtggattt gacaaagaaa gactggcgta agacaagtct aagagacttt
     2041 atttggataa gggaaccatt tcatgaaggg aaaaacaata caggtaagac aattgtattt
     2101 ggacatacga ttacaccaat gctccatgga gatatgcaga caacagacct ctggattcag
     2161 gatcataaaa tcggaatgga cggtggggct gtattcggag ggtcattgca tggggttgtt
     2221 tttgacaagg aaggtattct tcaagatatt gaatatcaga acaacgaagg accttggcat
     2281 ggaatctgac taatggaaag aatgaaggag ctgtgacttt gttgcggctc tttttatttt
     2341 tatagagaat attgtttctt tcagtagttt aacccaggat aaaaagtatg atagaataaa
     2401 gaagcaattt ttagcagaaa agaggcattt taatgacgga aaatttgaac cagacgatca
     2461 ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa gcagttgact actgtactta
     2521 atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg ttaccgaaaa gaaatgactg
     2581 gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg gtatgcctat cttgaaaact
     2641 tagaaaaacg taaaactgaa gtgattcggc taatcgatga acaagggaaa ctgactcccg
     2701 agttagaagc agaaatcacc caagccgtga aaatgcaaca agtggaagat ttgtatcgtc
     2761 catacaaaca aaaacgacgg acaaaagcaa cgattgctaa agaaaaagga cttgaaccac
     2821 tcgcgaagtg gctgatgcag cttacagatg gagaggtgca atctgaagca gaaaaatata
     2881 ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt gcacggtgct catgagatta
     2941 ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg gattcggtct tatacgtata
     3001 acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc agatgaaaaa ggcgtatatg
     3061 aaatgtatta tgactttgca gaacccgtcc ataaaatggt ttctcatcga atattagcta
     3121 ctaatcgtgg agaaaaagaa gatattctga aagtattctt tcaagtagat gaagcagcaa
     3181 ttttagctta cttggatcgc caaatagtca aaaatcctgc ctctccttct tcttcattcg
     3241 tcagagaagc ctatcaggat agttataaac gctttattca gccagcaatc gagcgagaac
     3301 tgcgcaatga attgactgaa aaggcagatg agcaagcaat cgctattttc ggcgaaaacc
     3361 tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt tgtcctggga tttgatccgg
     3421 cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac agggaaagtt ttagcaatcg
     3481 aagttattta tcctcataaa cctgcagcgc aagcaaaaag agaagctgct ggaccagctt
     3541 ttatccaatt aattaataaa tatcaagtag atatggtagc tatcggaaat ggtacagcca
     3601 gtcgggaatc tgaacttttt gtggctgagc agttgaaatc cgccgaccat aaaacctatt
     3661 acgctatcgt caacgaagca ggcgcttctg tttactccgc tagcgaaatt gcacgaaaag
     3721 aatttccgca tctacaagtt gaagaaagaa gtgctgtttc tattgcccgt cgcctgcaag
     3781 atcctttagc tgaattagtc aaaatcgatc ctaaagctgt cggagtaggc cagtaccaac
     3841 atgacgtttc tcaaaaacgt ttggctgaac aattggattt tgttgtggaa acagcggtta
     3901 accaagtagg tgtggatgtg aatacagcaa gcccgcagct cttgcagcat atatcaggat
     3961 tgaataaaac aactgcccaa aacattgtca tttatcgtga agaaaacgga gaattcaccg
     4021 cacgaactca attgaaaaaa gtgccacgct taggaccaaa agcatatgaa caagcaatcg
     4081 gattcttgcg agtccctggt gggaaaaaca ttttggacaa cacaggtatc catcccgaaa
     4141 gttattctat tgcaaaagaa atattaactt cagtccaact ttcagaaaaa gagcttggta
     4201 cagaagaagc agtagaaaaa ttgacacgat tatccgtaga gaaactggct gaaagcctat
     4261 cagtcggaga agaaacgtta gccgatattt tagctgggct gacacaacct ggacgagata
     4321 tgcgtgatga gatgccagcg cccttattac gtacagatgt attgagtatg gaagacttga
     4381 aaccagggat ggaattgact ggaacggtcc gtaatgtcat tgacttcggc gcatttgttg
     4441 atatcggtgt gaagcaagac ggacttgtcc atatatcgaa actcagcaaa aaattcgtca
     4501 agcatcctac agatgtcgtg tcagtaggag atatcgtaac agtttggata gaacaagtcg
     4561 atacgaaaaa aggcagaatc agtttgacga tgctttcccc ttatgaagag taacaatgag
     4621 gagtaacaaa aattgacaca agaagaactg cagcagttag tagaagagat atcattaaaa
     4681 agcttccagc ttcctttcaa gcaccaagca atgttcaatc cacgcttacg tacaactggc
     4741 gggcgctatc atttaaacga tcatcattta gattttaatg cgaagctatt tgaagagtca
     4801 gacgaagaga cgattgcagg aatcatcaaa catgaacttt gccattatca tcttcatcta
     4861 gctggaagag ggtatcagca taaagatatg gatttcaagt atcttttgaa aaaaacagga
     4921 gggttgcgct atgcaccagc cttgacgaag aaacagataa agatggagta ttatcgatgc
     4981 caagattgtt ctgccacgat ctgccgcaag agaagaatcg atgtacgcaa atatcgttgt
     5041 ggaagatgtc atggagaaat caaatggatt gagacaaaaa gagaagactt taatgaagca
     5101 caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa gtgagcagag gaatctaaaa
     5161 gactggaatc agctattaaa ttttttagcc gcacctgaag agggaaattt tgaggaactg
     5221 acggcaccta cacttatttt agctggaaac tgtcttccta tcttagctga taaagcagca
     5281 caaatgtatt taaatggcca agtagatcaa ttgtttttgg tcggaggggt ggggcatgcg
     5341 actagaatcc tttatgagaa ttttgaaaag caaggatttc attttgaaga tggcatgagt
     5401 gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg atttgcccga taaggctttt
     5461 ctgatagaat cgaagtctac aaattctgga gagaacgcca ctttttcact ggagattctt
     5521 cattccttag gagctgtacc agaaaaaatc ttattgatga atgatccgac actgcaacgc
     5581 aggactcgag caacttttga gaaagtatgg caaaatgaac aaactatttt cgcgaatgct
     5641 gtgccatttg tgccggaaat ccttcacttt tctgaagaga tcatctttac agcaaaagaa
     5701 ctgaatcatc aatggcctaa ggaatatttc catgcgctag tattaggaga aatggaacgt
     5761 ctccatgacg atgagaacgg ctatggtccg aaaggaaaag attttattcc tcacattgat
     5821 atatcagagg aagtttggtc ttcttacacc agaattaaag agtcaataaa aacggatttt
     5881 tcccgtactt gaccatttta gcaagcgaat cctcctttcc aaataaattt ggttgaaatt
     5941 ttcaatcgtt tataagataa tgaggagcgt gttgaatgta atagctaaaa gaatggacgc
     6001 tagataagag aagaggattc aaatggaaaa tcaaaataaa ttgtatcatt ttgttggtat
     6061 caaaggttca ggaatgagct ccttggctct tgttttacat gaacaaggtc taaatgtcca
     6121 aggatcagac attgaaaaat atttctttac acaaagagat ttagaaaaag caaatatcac
     6181 gattctgcca tttaacgcag ataatgtaaa accagggatg acgatcattg caggaaatgc
     6241 atttcctgat tcacatgaag aaatccagcg agcaaaagaa ttaggtttgg aagtgattcg
     6301 ctatcacgac tttattggtc attttatcca aaattatacg agtatcgctg taacaggctc
     6361 tcacggaaaa acaagtacga ctggactgct ctctcatgta ttgagtggtg tccgtccaac
     6421 aagttatcta attggagatg gaacaggaca tggtgatccg caagcggaat tcttttcatt
     6481 tgaggcctgt gaatatcgcc gtcatttcct tgcctattca ccagactatg caatcatgac
     6541 caatatcgat tttgatcatc cggattatta tacaagtatc gacgatgtat ttacagcttt
     6601 ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg tatggtgacg atgcttattt
     6661 acgcaaatta aaagcaaatg tgccgattta ttattatggt gtcacagaaa acgatgatat
     6721 ccaagcacga aacatcgaac gcacaacttc tggttctgct tttgatgtct atcatgggga
     6781 tgaatttgtt gggcacttca ctgtaccagc attcggtaaa cacaatatct taaatgcttt
     6841 aggtgttatt gcagtagctt atttcgaaaa actggatctg aaagaagttg cagaagaaat
     6901 gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag atcgtagcag acatgaccgt
     6961 agtcgatgat tatgcgcatc accctgcaga gatcaaagca acgattgatg gcgcacgcca
     7021 aaaatatcca gataaagaaa taattgcagt tttccaaccc catacgttta cgcgtacgat
     7081 tgcattgatg gatgaatttg cagaagcact tgatttagct gataaagttt acttatgcga
     7141 tatttttggt tcggctcggg aagaacaagg aaacgttaag attgaagacc ttggagcgaa
     7201 aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta tctccattgt tagattatca
     7261 tgatgcagta gtaatcttca tgggtgctgg agatgttcaa aaatttgaac aagcgtatga
     7321 aaaactttta agcagcacga cgaaaaatgt actgtaatac aattactgca gtgtccttat
     7381 aaaaatagaa agagggggag tggcaaaaat cttgccaagc cccctttttt gcaacttatg
     7441 ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc caatcaaatc aaaaacatga
     7501 gaagctgttt ttcttggctg ttcttattac ttgatgcagg gcagcttttt cacgacctca
     7561 gataaacggt gttcaaaagc ttgcatctgc ggtaataagt ccagccaaat gaaaaatatg
     7621 agaagctgtt ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct
     7681 ctgtaaacta taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt
     7741 ggtacaataa tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc
     7801 cgagaaaact aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag
     7861 aatctattga agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt
     7921 tgtatatcca acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag
     7981 tgatgctgat ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac
     8041 atgttgtcaa tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt
     8101 ttcagtttga ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag
     8161 cggagaatac gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag
     8221 ttcctgaatt ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca
     8281 tcaatgtcag gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt
     8341 gcccttagtg cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact
     8401 tttatcaata attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta
     8461 atggttctag gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc
     8521 tactctcttt tgaatggcat aacattagcg gttactcttg catactacga tattggaaca
     8581 gtaacgcaag ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta
     8641 acaacaaaaa gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg
     8701 atcattgcac tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc
     8761 tccctactgt tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt
     8821 aatatctatg tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg
     8881 aagctgtatt tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac
     8941 aactaaaaaa gaaaaaaatt catcagttgg ggctataccg aattatttgg tatggtcccg
     9001 ctttttcttt tcgcattccg caggattctt tgttagaatg aataagaact ttacagtaag
     9061 gagttaagaa gatgacaaaa aataaattat tactcgttga tgggaacagt gtggcttttc
     9121 gcgcattctt tgcgcttcac aattctcttg aacgtttcaa aaatagaaac ggtcttcata
     9181 caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt tatgcaaaaa gagcaaccga
     9241 ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt tcggacagaa atgtatgcgg
     9301 agtacaaagg tggaagatcc aaaacacctg gcgaattcaa agaacaaatg ccatatatac
     9361 gtgatttgtt gacaggacta ggggtacagt attacgaatt gcccaattat gaagcagatg
     9421 atattatcgg tactttggca gaaaaagtag acaaagatca atttgatgtc gtcgtgcttt
     9481 caggtgatcg ggatttgact caattagcta gtaaagaagt aaaagtggat atcactgtaa
     9541 aaggtgtcag cgatatcgaa agctatacac cagaacatgt agcggaaaaa tatgatgggt
     9601 tgacgccaaa acagattatt gacatgaagg gactagccgg agatgcctct gacaatatcc
     9661 ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct attgaaacaa tatggctcag
     9721 tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag caaaatgaaa gaaaatctga
     9781 taaatgacaa agagcaggct ttcttatcga aaaaattggc gaccatcaat acttcagcgc
     9841 ctgtggatgt ctctatcgac tcattgaagt atgaagggaa aaatctcgat aaactcgttc
     9901 cgttttataa agaaatggat ttcaatcagt tcctttctaa attgaatatc gtagaagaag
     9961 aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga attcaaagag gaaatgttta
    10021 ctacggatat ggcgttgtac gtggaaatga tgggcgacaa ttatcataca gaggaaatcg
    10081 tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac gaacgatcta tctgtattcg
    10141 aaaaccgagc gttccattct tggatcactg atccgactcg gctgaaaaaa gtatatgatg
    10201 cgaaacgaac ttatgttgct ttaaaccgct atactggaaa aacgaaagga atcgattttg
    10261 atgtcttgct aggtgcatac cttctcgata cgaatgaaaa aagcacagat atcgaaggaa
    10321 tagctgctca ttacggatac aacgatatcc aatcagatga agctgtatac ggcaaagggg
    10381 cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc tcatttagcg cgaaaagtag
    10441 ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact ggcagataaa aaccaagaag
    10501 atcttttccg gaaaatggaa ctgccattgt ctatcatttt ggcagagatg gaaataagcg
    10561 ggatcaaagt agatgcgact cgcttacaag aaatgaaggg cgagttctct gctcgcttaa
    10621 gagagatcga acaaaaaatc tacgaagaag ccggcgaaga attcaatttg aattcaccaa
    10681 agcaattagg agtgatctta tttgaaaaaa tgggattacc cgttatcaaa aaaacaaaaa
    10741 ctggatattc cacagccgta gatgtactgg aacagcttcg tgaacaagca ccaatcgtag
    10801 aggatatcct cacctatcgt caaattgcaa aaattcaatc tacctatgtc gagggactgc
    10861 tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta tgtccagaca ttgacccaaa
    10921 caggacgatt aagctctgtt gatcctaatt tgcagaacat cccgattcga ttagatgaag
    10981 ggagaaagat acgtcaggca tttgttccta gagaaaaaga ctggctgatt tattcttctg
    11041 actattcaca aatcgagtta cgcgtacttg cgcatatctc aaatgatgaa catttgaaag
    11101 ctgccttcct agaaggtcaa gatatccatg ccagcacagc gatgcgtgta tttggaatcg
    11161 aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc aaaagcagtg aattttggta
    11221 tcgtgtatgg catctctgat tacggacttt cacaaaatct aggaatcagc cgaaaagcag
    11281 cacagcaata catcgatacg tactttgaaa aatacccagg tgtcaaagaa tatatggaaa
    11341 gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac gctgtatcac cgccgtcgat
    11401 atctgccaga catcaattca agaaattaca atatccgttc ttttgcggaa cgaacagcta
    11461 tcaatacacc aatccaagga agtgctgctg atattttgaa aatcgcgatg atcgaattgg
    11521 ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct gttacaagtt catgatgaac
    11581 ttgtcttcga ggtaccggaa aaagaacttg aatcattaga taaattggtg aaggaagtta
    11641 tggaacaagc tgtttctctt catgtaccac tgatcactga cagcagttgg ggaaaaacgt
    11701 ggtacgaagc aaaataaaca ttgtttaaga cagagcaata taatgaaggt gaaaagaaaa
    11761 aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga ttggaacggt tagtagtagg
    11821 aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc atcgagcaac cagagacacc
    11881 catctttgag gcttctcttg ttggcgaaac catccaatcc attggacgta gagggaagtt
    11941 tttaattttt catctcgatc attgtgaact gatctcacac ttgcgaatgg aaggaaaata
    12001 tcaatttacg aaagaaaata caccaatcga taaacatacg catgtacttt tcttttttga
    12061 ggatggaagc caacttcgct acaacgatgt acgaaaattt ggtcgtatga caatcgtcga
    12121 aaaaggagca agtgcaactt accgaggaat catgaaattg ggacctgagc ctttgccaga
    12181 tagcttctta ttagcagatt tcgcaaatgg actgaaaaag tcccacaaag caatcaagcc
    12241 attgctgcta gaccaacgtc tagtgactgg tctaggtaat atttatgtag atgaagcatt
    12301 atgggaagcg aagatccatc cagaacaacc ggcgaacaca ctgcgcccca aagaggtaga
    12361 acaattacgt ctatcgatca ttgatgtttt ggatcgtgca atagaagccg gaggtaccac
    12421 gattcgcagt tatttgaatg cattaggaga atcaggcgga tttcaagtag cactgcatgt
    12481 ctatcagcaa actggcaaac catgtatacg ttgtggaacg ccgatcgtga aaacaaaagt
    12541 ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg ctaaaaatcg caaagtagga
    12601 tttgttttag gtttgacggg tggaatagct accggtaaaa gtacggcagc caaagtcttt
    12661 cagtctcatg gatttccttt gatcgatggc gatgtgattg ccagagaaac tgttgagcct
    12721 ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc aagaggtatt gcagcctgat
    12781 gggcaactag atcgagggaa acttgggatg attgtttttc catcaaagga aaaacggcaa
    12841 aaacttgatc agctattaga tccgtttatc cgaaaagcga taaaagatca aatcgcctct
    12901 ctaagttcca atcatccttt agtaattgta gatatcccat tgctctacga aggtcattat
    12961 gaccactata tggatgcagt agctgtcgtt tatactacgc cagaaactca gcttcagcgt
    13021 ttgatgaaac gtaatcaact gacgctagaa caagcgcagc agagaattag tagtcaactt
    13081 ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg acaacaatgg aacaaaagaa
    13141 aaacttgtgg gacaaatcga aaattggctt tttgctaatc gctttctttg agtatctgtg
    13201 tcaattttgc tatcctgagt atatagaaag tgagggatag cgatgggatc attaaaaaat
    13261 caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg gcgagatgct caataaagaa
    13321 aatctaatcg atgaaggtcg tacgcaatca gaaacagcag aagaaaaaga aaagcagtac
    13381 caagcaagaa tcgatgaaat agaagaccag caggcagaag aagaagcgaa gcggcaagat
    13441 ttgactcaag aacgaaaaaa tccgacacct gatcgtagta aaacaccgct cccagaaagt
    13501 gaccacattt ccgatgagca aaaaatcgag gaacagcgaa tgaaacacta tacaggaatc
    13561 gaagagaagt tataaaatag taaaaaatcg agatgtgact tgaagcagat gagaaggtgt
    13621 tcatctgctt caagtttttt gtgctagatg actaaatcag cttttgtgta agttatttct
    13681 cagtaatcaa ggtgatcagt attggaaaat tgaaatttag taaggacagg ggacacaatt
    13741 acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt tttgggtagg acagtcgcgt
    13801 ttgtgatttg tttcgtgtta taatacaact agttgtaaag aaagacacat cctttatata
    13861 aaatgcgatg caagaggtga gacaaaatga gatgtcccag atgtcaacat aataattcac
    13921 gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat ccgtcgccgt cgcgaatgtg
    13981 agaattgttc gttccgattc actacatttg aacgaattga agctgctccg ttattagtaa
    14041 tcaagaaaaa cggggaacgc gaagagttca atcgcgataa aattttaaga ggactcatcc
    14101 gttcagcaga aaaaagacct gtagctatgg aacaaatgga acaaatcgtc aaccatgtcg
    14161 aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac gaatctaatt ggtgaatatg
    14221 tgatggaaga tttagttgat ttagacgaaa tcgcttatat tcgttttgca agtgtttatc
    14281 gccagttcaa agatatgtct gttttcttaa aagagttgca agatatcgtg gataaagcaa
    14341 aagaaaataa taaagagaat aataaagaaa acaattaaga aacaaaaagg aggcatttcc
    14401 ttggaaaatg cttggaaaga gcttcaacca aaaaatattt accaaggaag aaaagcggct
    14461 ccgctgagag aagacagcaa gaacgccttg ctgtatcttt atcagccact aatcggcgga
    14521 gaagccttga gtatctattt caccctgtta tcagaaatct ctcaagaatc tggcaaaggt
    14581 cctgaaggga tgcatgcaga tctgcttagt agtctaggct gcggtatccc gcaattttat
    14641 gatgcaagaa aaaagctaga aggaatcggc ttactggatg tttattttaa agaagatagt
    14701 gatctgggac ctcgttttat ttatgagctg ttagaaccac tcattcctaa agcatttttc
    14761 aaagactctg ttctggcttt tcttcttctg gaaaaagttg gtgaacgacg attccagaag
    14821 cttacccaat tctttgaacc taaaaggcat acagcagagg gatatcaaaa aatcacaaaa
    14881 aaatttttag atgtttatcg cttcaatgaa gcagtgtacg ctgctaatca agaaaacatc
    14941 gaatcgctga ataaatcttt tgaagaatct gaatcagtag cattacttga agcagattcg
    15001 acattcgatt ggcagttttt gaatgattgg ctggcaaaaa aacatattgc aagtcttgat
    15061 gatccaatca tcaaacaagt cgacatgtat catcagcttt acggtttcga tgaattgaca
    15121 cttggcgaac tgatcagtca atcttatgac tttagtgaac aaaaagtatc tgaaaaagaa
    15181 ctgcaaaaag ccattttggc aatgcagaga cgtccggtga agcaaacaaa agaaacagtt
    15241 gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac atttgacaaa cgcagcgctt
    15301 cagctgataa aagaagcaca gagcattcca ccgatgaaat atctggaagc aatcaagtca
    15361 gaaaaaggcg gatatgtttc taaacaagaa aactggttac tgcaagatct tgtttctcaa
    15421 tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt atgtactagt gatcaagaat
    15481 aatgctagct taaatgccag ttatgtaaat acagtggcaa atgaatgggc acaaaagaaa
    15541 atcgccacag cagaagaagc gatccagcac attcggcaaa taagtaatca agcaaaacaa
    15601 tcaaaacaaa agaagcaaac aaattatcaa actggcaagc gaaatgttcg acgggaaaag
    15661 cttcctgatt gggtcaacca gccaaaagat gaaaaacaga tcagcagcga gaaaaaagca
    15721 gagattgacc gccgttttaa agaatacctg gcaaaaaagg agggtgatat ctagtggaag
    15781 acgtaggcaa agaattgaag aggatcatca gcaaacgcaa ttatcaagaa aagtatgctg
    15841 aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc ttttctaagc gcacaccaag
    15901 aagaattgac acaagcggat attgaaaaaa gttacgctaa attgtatgaa ttcgtgcaag
    15961 aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc tcccggctat gagccgaagc
    16021 tgacgttgaa ttttcattat gtggatgtca cttatgtacc aacggaagca ttgttagcac
    16081 ggcaaagaca agaagaaatc agaagccgag taaaagcaat ggatatgcca aaagatattc
    16141 aatcggcttc ttttgaaaac tttgaacgta cagatggacg aggatatgcg agcttagaag
    16201 ccctagattt tgtagagaaa tatactgctg atccaaaagg gtatcataaa ggactgtatc
    16261 tagtaggaag ttttggtatt ggaaaaacat atttattagg agcaacagcg agagaattag
    16321 cgattgctgg attcaccacg acgctagtcc attttccgac atttgccgtt gagatgaaac
    16381 aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc agtgaaacgt tcacctattt
    16441 tgatgatcga tgacattggt gcagatgcga tgagtagttg gatacgagac gaagtattcg
    16501 gtgttatcct tcaatatcgg atgcaggaac agttaccaac cttcttctca tcgaatttta
    16561 caatggatga attggaacag catttgtccg tcactcaacg tggagatgaa gagccgctaa
    16621 aagcgaaacg gataatggaa cgcatacgtt atttgacaaa agaaatcgaa atgactggtc
    16681 gcaaccgacg caatggttga acagttgaca agtccaactg acatttgacc tagtagtaaa
    16741 agcaggtcag cgaatcatta tatggacaga aatgaccgga gacaatcacc gcaacaagta
    16801 atgcagaaac ttaaacggag gattttttat gagggctatt ttttctagta tatctgtagt
    16861 tggtctattt tttcttttag caggctgcgg tagcgcagat agcaacacag gttctgttga
    16921 ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg aaaacggaga gacaaagcat
    16981 atttgtcgga acgattgaaa gtattactca agtagaagca gaagaagaaa cgcttcagtt
    17041 gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat atgggaacgt cattcagcaa
    17101 tgacggagtg atcttaaatg ctactgctga aaaaataagc gtggacataa atgaattgaa
    17161 aatcggtgac aaagtccaat ttacattagt tgagatgccg atcatgacga tgtctatccc
    17221 accgcaagtt cctggagatt cgattattga tgtttcattg aacaaataac caataacatt
    17281 ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag gtatattttt taaggagata
    17341 tggg
//
LOCUS       DACDTO010000059        17081 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDTO010000059 DACDTO010000000
VERSION     DACDTO010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.059"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(366..605)
                     /locus_tag="IWK70_001941"
     CDS             complement(366..605)
                     /locus_tag="IWK70_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614461.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            891..1205
                     /locus_tag="IWK70_001942"
     CDS             891..1205
                     /locus_tag="IWK70_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614462.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(1639..2005)
                     /gene="ssrA"
                     /locus_tag="IWK70_001943"
     tmRNA           complement(1639..2005)
                     /gene="ssrA"
                     /locus_tag="IWK70_001943"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2176..2949)
                     /locus_tag="IWK70_001944"
     CDS             complement(2176..2949)
                     /locus_tag="IWK70_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9614463.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            3093..3443
                     /locus_tag="IWK70_001945"
     CDS             3093..3443
                     /locus_tag="IWK70_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9614464.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            3424..4140
                     /locus_tag="IWK70_001946"
     CDS             3424..4140
                     /locus_tag="IWK70_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614465.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            4140..5588
                     /locus_tag="IWK70_001947"
     CDS             4140..5588
                     /locus_tag="IWK70_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9614466.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(5658..6167)
                     /locus_tag="IWK70_001948"
     CDS             complement(5658..6167)
                     /locus_tag="IWK70_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614467.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(6157..6882)
                     /locus_tag="IWK70_001949"
     CDS             complement(6157..6882)
                     /locus_tag="IWK70_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614468.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(7105..9225)
                     /locus_tag="IWK70_001950"
     CDS             complement(7105..9225)
                     /locus_tag="IWK70_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9614469.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            9455..10633
                     /locus_tag="IWK70_001951"
     CDS             9455..10633
                     /locus_tag="IWK70_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9614470.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            10649..11407
                     /gene="aroD"
                     /locus_tag="IWK70_001952"
     CDS             10649..11407
                     /gene="aroD"
                     /locus_tag="IWK70_001952"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9614471.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            11430..11915
                     /gene="ybaK"
                     /locus_tag="IWK70_001953"
     CDS             11430..11915
                     /gene="ybaK"
                     /locus_tag="IWK70_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9614472.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(11986..13239)
                     /locus_tag="IWK70_001954"
     CDS             complement(11986..13239)
                     /locus_tag="IWK70_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9614473.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            13357..14706
                     /gene="gdhA"
                     /locus_tag="IWK70_001955"
     CDS             13357..14706
                     /gene="gdhA"
                     /locus_tag="IWK70_001955"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9614474.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            14818..16164
                     /locus_tag="IWK70_001956"
     CDS             14818..16164
                     /locus_tag="IWK70_001956"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9614475.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(16472..16858)
                     /locus_tag="IWK70_001957"
     CDS             complement(16472..16858)
                     /locus_tag="IWK70_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9614476.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(16907..>17081)
                     /locus_tag="IWK70_001958"
     CDS             complement(16907..>17081)
                     /locus_tag="IWK70_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9614477.1"
                     /translation="TVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQ
                     RRFEGTLRYSLGY"
BASE COUNT         5584 a         3001 c         3398 g         5098 t
ORIGIN      
        1 ataaagattt aaataaagtg ccttcaaaag cgagaagacc aagcttttga aggcacttta
       61 tttagttggc agtgattgtt catgattttt gatttattca aaaggttcgc ctagagaatc
      121 tgtctctgta acgaggcctg gtggagaaac tcgataaaaa cctatactgc cagaactgtc
      181 ctattttcgg atagacaaac tagtagtttt aaaaagatca tccgatccaa tcatttcaaa
      241 aaatgcaaaa ctgatatcag gatcatttag ctcaggcttg ttatcgtaaa gcatttgtaa
      301 aggttgcatt tagttaggag taaacgcgaa actatcatca ttatttgctg aagaagatga
      361 agaagctaat tcagagtttg tttgaaataa aggagacgtg ctgacagttt ctttaccctg
      421 ttttttgcag aggtagaaac ttttatatcg gaagtttcta ccggagaatt tttctcattt
      481 gtagaaattg ttgaatcgat actattcgaa atttgcgtat ctgaaggtat gttctttgat
      541 gcagaagtcc cacaagcagt aagtaacaat aaatttgata aactgataaa cagaattctt
      601 ttcattatgt acacactcct tatagtatcg tttagcatac acctatgctc agtattaggc
      661 aaaatcaatg caaaactatc aagtctttta tagtatctat tagtcttagt aatacatata
      721 tggagagggt gatatcccag ttcaaaccag atacattggt taccatctgt attaacgatt
      781 atgcctctaa acgctttttt tatgaaaaaa tttataagtg aaaacgtagg tatgatatta
      841 tctagaggta atttttttgg gtatttaaat agttgctagg aggatagctt ttggattttt
      901 acagtaattt tatcttaata atagcaatat tattacttct aaatatttgg ttttttgata
      961 agagtagaaa tgcagggata ggttttagaa ccaaacgaag tacgtcttct gaaaaaaagt
     1021 gggtatattc acagactatt ttttacggag gggttatttc aatcagttta ctttcttcaa
     1081 cactttattc ttttaatgtt atagacgttt ccatgtcaaa ttttatatct attattggaa
     1141 tccttatttc tgcaattatt acacaactac ttcttgtgtt tgaagagaag tcaaaaaata
     1201 attaaagcaa ttatgccttt tagtacagga attattactt cttacaaatg tatagcgaat
     1261 gtgaagcctg ctgttttaat tgttctagaa aaacttttga gtgaacaatt attttatgtt
     1321 aggatgaatt aatttctcta gagtttataa ttaaaaaact tatttgaagg aacatgctta
     1381 aaccataatt gtgtttttcc ttccatcagc ataaaagttc acctcaaaaa gagtgtacga
     1441 acaaaagttc tttttgtaaa ccttaaaata gattttcctg aatacgtatt ggggatttct
     1501 aaatgtttga atgttttttt gaatgcaaaa tcaatgcaaa aaattttgta tttctctact
     1561 tttctctcct tgaccaaaat aaaaaaacgt tgatttaaca acgtttttca tacatgttta
     1621 tctgtctcaa ccatacatat atggagacgg cgggagtcga acccgcgtcc aaacacatcg
     1681 ccacttaaaa atctacgttc atagtcaact catttaagga ttcactttac agcttgccga
     1741 gtgacaggca ttctgtatcg ctagtctgat gttctctttt tcaacttaca gacggaaagt
     1801 tgaaacgtat cccactaaag ttaggaccct tactcgagca catgggcgat gccgagagga
     1861 tctacgctaa ctgtttttag gcagctaaag cgtaagagtt gttttcgttt ttagcagtta
     1921 tatttaactg taacgttttt acgtagacgt aacctacgaa acgcaattca agctcgactg
     1981 tgcctgtcga atccgtaacg tccccgttag ctaacaaggg ttacgtgtat ttttcataca
     2041 aaattagtat agcatatatt gggataaatt caataaaaaa gcgaatcata tcccattatt
     2101 tatataataa ctgccccttt cttaagaaaa aataagcgat tttcttaaga aaagaggtag
     2161 cttcaaaaaa agatcttaaa tcagatcaaa atacttcaat gcgtggacaa ttccgccttc
     2221 tgtatttttc tttgtgataa aggtagaaag ttcttttaat ccgtcatagg cgttgcccat
     2281 agcgattttg ttgtcgcaag cttccaacaa ggcaaaatca ttcggaccat cgccaaatcc
     2341 aaaagtagga actttttcca gctgaagttc tttttgcaga cgtttgacac ctgccccttt
     2401 tgagattcct ttcttcactg tatcgatcga gtaaggacca ttccgataaa aagttagctc
     2461 aggaaaatgt tcgtgatagt attcgtcacc tgattgagat aagatcagca acatatttac
     2521 tgtgttttct ttaaagccta aaggatcaat ttccggaaca tctgaatgaa tgaagtcgta
     2581 agcattgatc attgtttggc tatgaccggt acaccagatt ttctgatcgt tatagtaaga
     2641 aagttcatga ccgttcaact gtacatgatc atacattttt tgacattctt ctgaagaaaa
     2701 tgtttgagaa tagatttctt cgccgtctac tcgaatgaaa gaaccattca tggtgaccgc
     2761 agaagtaatg cccgcatctt ccatgataga gtggatttct gcttctgtac gtcctgtagc
     2821 gatgacgggg agaacattgt tttcttttaa tgcgttcata gcttttgcca cttcgggtgt
     2881 gatttttgat ttcgcatcta gtaatgttcc atcaagatca aagaaagtga ttgcacgata
     2941 atttgacaca aataaaactc cttttttatt ggaataagtt tatcttaaca aagaatgaac
     3001 aaaagacaaa cctttcgtga agaattcctg aacatattgg ttcagtcaac aatttagagt
     3061 atactataag aagagaattt aggagggaaa gaatgatttt tttcagtatt ggtatcttat
     3121 tcttaataat tggcagtctt tttcggattc tgccctcaaa aggaaatctg cctttttatg
     3181 ggtatcattc gcctcttgcc gcaaaaacag acgctcattg gcggctggct caaaagacaa
     3241 gcggaaactg gttttttctt atgggcttac tgatggcatt gatcggctat tatttaaaga
     3301 catctgggca tacgaattat tttctgattg aaatgcttct gctagtattt ccaattatgc
     3361 ctatttttat tatgacagaa aagaaattac agaaatatga cctggaaact ggagggaacg
     3421 ataatgaata ttttaatgat tgaagataat caatctgttt ctgaaatgat gcagatgttt
     3481 ttcttaaatg aaggttggga agcaactttc aagtatgatg gaaaagaagg gctcgatgcc
     3541 tttttagaaa acccacaaca ttgggatatg attacactag acctgaattt gcccacactg
     3601 gacggtatgc tagtaggaag agagatccgc aaagtttcaa gtactgtgcc gattatcatg
     3661 ctgacagcta gagattctga aagcgatcag gtgatcggct tagagatggg ggcagatgat
     3721 tatgtcacaa aacctttcag tcctctgacg cttattgctc gaatgaaagc tttgcatcgc
     3781 cgtagtgagt tggcagaagc aagagaagaa gcagcagttt cagatgatca ttttgatatc
     3841 gagactagtc atttcaagat gaatacgaaa acacgagaag cgtatctgga caaccggcca
     3901 attgaaggac tgacgcctaa agagttcgat ctattgttta ctttagcaaa aaaaccacgt
     3961 caagtattct ctcgggagca gttgcttgaa ttagtctggg attatcagta ctttggtgat
     4021 gaacggacag tggacgccca tatcaagaaa ttacgacaaa aaatcgaaaa ggtcggacca
     4081 caagtcatcc aaactgtttg gggagtcggc tacaaattcg atgattctgg tgttgcctaa
     4141 tgcgttattt gtatcagcaa ctcttagctt tttgcggaat ggtcgcgttg atcttattga
     4201 tcgttggagt ttcttttaca caactgacaa aacaaacgat tgaagaaaac aattaccagc
     4261 aactgtttgg atatgctgaa tcagttgaaa agacaacgca aacctatgcc gatacgctcc
     4321 cgcagtttag tcaagatcag gcgtttcaaa atgcattgct gctgacagag caagtactga
     4381 cagaacaaaa tgtgaacttt atatttatcg ataaaaacga acgtgtcatt tatccgacta
     4441 atacagcttt actgaatttt accttaacag ctgatcaatg gaaaaatctg aggaatggac
     4501 gtcaagaaaa attcacatcg aataaaaata ttttaggaca agcacaggcg acctcttacg
     4561 cacttgtgcc atttaatttg aatcatgaat tctatggagc acttgtagtt agtcaacctg
     4621 cgcgaaatat cgataacagc gttcgtccaa ttacactgaa cctgttcaaa ggatttattt
     4681 tttccagtat cgttgcagtt attgccagct atgcttttgc agcattccaa gttaagcgta
     4741 tcaatcggtt aaggaatgcg acgaaagaag tgacgaatgg taattttgat gtacagctcc
     4801 ccgtacatga caaagatgaa tttgatgagt tagcagatga tttcaataaa atgacgaatt
     4861 cattgaaaga atctcaagca gaaattgaag aacaagaaaa tcgtcgaaga caatttatgg
     4921 cagatgcttc tcacgagatg agaacacctc ttacgacaat caacggactt ctggaagggt
     4981 tagaatacaa tgcaatccct gaaaatcagc gagaaaacgc gattaaattg atgaaaaatg
     5041 aaacagagcg tctgatccgt ttggtgaatg aaaacttgga ttatgaaaaa atccgtacga
     5101 atcaaatcag tatggtcatt aaaaaattca atggaacaga aacattgaaa aatattgttg
     5161 cacagttaga agctaaagca gaagcagcag gagatacatt gacattgaaa gccgttgatg
     5221 atattgatgt ttacgctgat tatgaccgat ttgtccaaat catggtcaat attattcaaa
     5281 acgcgataca gtttactgaa aacggccaaa tcacgattac tttagaaaaa ggctatcttg
     5341 aaacgatcat cacgattgaa gatacgggaa ttggaatgtc tgaacaacaa atgaaaagta
     5401 tctgggatcg ttattataaa gtagatcctt caagaaaaaa tacaaaatat ggtgaatcag
     5461 gattaggttt accaatcgtg cagcaacttg tacgcttgca taaaggaaaa ttagaagtag
     5521 aaagcgaact aggaaaagga acaaaattcg ctgttcgttt gcctgacgta gaaatcaaaa
     5581 aagagtaaaa aacaagcggt ataaaagatc atgaatcgat cttctatacc gcttgttttt
     5641 actataaaat gattgattta gcccgcgggc tctaaacgat caatataagg actttgagca
     5701 atagtagcat catgagtaaa gagtaagata ccttgatttt cttcatgagc taaataagaa
     5761 agcaagtgga gtagctcttg gatctccaat acactaagtt catcaaatgt atcttcaata
     5821 acaatattgt ttcgcttgga aagcaaggca tgaagtaatt gcagctttac tttttcaaaa
     5881 gacgtcaact cagctgccgc acaattaagc aaggaaggag agatatcaag tttttcttga
     5941 taggccaaac gatcttgttt gatttctttt tccggtacat ccaacaggac atggtcttta
     6001 agattcagat aaggaaacat cggccaattt ttttggatga tcgcaaaatt atccagtttt
     6061 gcaacaagaa cctctttttt caagtgctcc aatattttat gctgtttttc gagaggacac
     6121 aaaataacgg tcaagtgttc agcatgaatc aaatattcag gtttcattac gtccaggcct
     6181 cctactttgc aaatgatgga taaaaaaagt gaaagggaat agactaagaa tgagacagca
     6241 tccactgagc ccaataagag tttgggcaaa tcccttaagt atatctgtga aaaaaagatc
     6301 attttggaaa tcgatggtaa aaaaagtctg ttgatccatc ggaattgtaa gtccgcttac
     6361 cgaagtcaag atatcacgga tatctggcag ttcagtagtc tttaagacaa gaaaattgat
     6421 cctttgaaga agcgcttcat aaactgactg gaagcttacg atccacaata aaaccaagat
     6481 acaacaaaga aagagaggaa agaacagctg ccagaaaata aaaaggaata atttggtttt
     6541 agaaagacca gtcaggcgcc aattgatcaa ttcctgcttg ttttgcctga ctgaatagta
     6601 aaagaaaaag ataaaaagga aggcaaatag aaaaaataaa ctgaatatgc cttttatata
     6661 aaatcggcta gctaattcta aagccggaag aagctctgta gatgcagaag cgccaataaa
     6721 tgaaagccga tcttggactt gattggctaa agccgattgc aaatggtaaa gagtcaggca
     6781 tgctatcaat aaaaaagcag aaaagcttga aaataaacaa tagagtattg tctgcttttt
     6841 gtaataaatt aatcgagcag tagcatagcg gattattttc atacttttca cctctataaa
     6901 aagtatagaa aatgaatatg aataaagtat gaaattcctt tagtgaaatc acgaattaaa
     6961 accagaaaat attttgtaaa aagcaagagg ttgtgaagaa gctgccctat tctattaccg
     7021 tagatgcaag cttttgaaca ctgtttattc gaaaagagga gcagcgacaa taattttgtc
     7081 gctgctcctt ttaaatttat tttgtcattc ggaagaactt gtggtcgttc cgtgaagttc
     7141 ttggtacgtt tgcgttttat aaagatcaac agttgattgt ccgttgtgtt cactaaagac
     7201 actagttgac ttgtcaccaa gagatttttc agcttctttt aatttctcta agccattctt
     7261 gtagtcataa tcactggtat caattgggtc taatccacta ttcgtataga accgaagaag
     7321 atcaccatta ttgatttggt cagaaacagc taactgttta tcaacttttt ctttcagtga
     7381 atcaatttca ttctgtactt cctcagtagg ttcagtaata gcttcaccag tttcgttact
     7441 atagatcgta ccaccgtagt aagtatattt tggtgtaaca aaatcaccat cacggaaaga
     7501 aacaagctgt tcatgatctt tcgagaatag atcttgtcct aactgaatat aattttttgt
     7561 atctacacct agtaagtgaa gtaatgtagg caaggcatca acttgtccgc cgtaagtgtg
     7621 attgattccg ccctgtgttt gtccagggat atggatcata taagggacac gctgcatttg
     7681 tgcattgtcg tagtttgtcc aagtagaact tgtttttcca actaattcag ctagattttt
     7741 atttcttgaa ttcgagacac cgtaatggtc accgtaaagg acgatgactg aattatcata
     7801 caagccgctt tctttcaaat aattgaagaa ttcttgtacg gcggtatcta aataattggc
     7861 tgtcgcaaag taaccgttga tcgtttcatc atttgtattg gctaacggga atcctgcttc
     7921 gtcattggtg aattttgaat aaggataatg gttagataca gcaatgaatt ttgaataaaa
     7981 tggttgttgc agacgttcca agtattgtac ggattgttgg aaaaatggtt tatcatgcaa
     8041 cccgtattgg aacgaattgt catcattaac atcataataa gaagaatcaa agaaataatc
     8101 atatcctaaa ttcttgtatg tctcgtttcg attccagaat gtaccagcgt tcccatggaa
     8161 taccgcacta gtgtaacctt gtgtttgact taaaatatca ggtgcagctt ggaaagtatt
     8221 ttttccgcct aattgtgtga acaaggagcc ttgattcaag ccaaacaatg aattttccat
     8281 cagcgtttcc gcatcgcttg tttttcctgc ttttacttga tggaaaaagt tgtcaaaact
     8341 gaacgtgctg ttgctgtggt aaagactatt gatgaaaggt gtaacttcat gctggagacc
     8401 attctcatct gtcaatttat aatcgatcag gaattgctgg aagctttcta aatggatata
     8461 aatcacattt tttcctttag cgagcccgaa caaatcgtca tttggtgctg cataatgttc
     8521 ttttacataa ttttctactt ctttcatatc attgggactt gcttctgccc gcacttggtt
     8581 tgtctggtaa gtttgaacac cgtcataaac catgaaagca ttcaagccaa gatatttgac
     8641 caaataatca cgagagaatg ttcgagtcag caattctgga cgatcagctt ccgccataaa
     8701 aagatttcct gagaaaatca taactgctaa tgtagagatc gcaaaagcca ttcttgctcg
     8761 gatcgggcga gggtccaact tgattttttt tgcaatcagc agaacagtca agataataaa
     8821 atcgatccaa tagatcacat catatgggcg aaacagtcga atggcacttt ctcccagacc
     8881 actcgctact ttcccagcac ctaaaatcgt gttgatcgta atgaagtccg tgaattctcg
     8941 ataataagtg acattcgaaa aaacaagcaa tgtcaaaaga aaataaatga ccatcatcgt
     9001 gatataagca actctagggc gtctgatata aagagcaatt ccaagtaata acaatgtagt
     9061 tgcaataggg ttgataaaca agataaagta ttgaatgcca ctttcgattc cgagatggaa
     9121 atcaactaaa taggcaaaaa tcgttttcaa ccaaagtaaa acgactagta aagaaaaaaa
     9181 tccgagcctc ttattcaaaa aggcaggtgt ttttatacat ttcaagataa tcttcctttc
     9241 tgcacttctt aatcacagaa gtgtcatgaa accatgttac acagctcact tattctcatt
     9301 tattttacta gtatgacaat atagtgtcaa ttgaaaccag aagtttatgc tgtttcatta
     9361 aggttttctt taagcttcat gaaaaaatga gcagtaagac aagtcctatg ttttttgtta
     9421 tacttgggaa gagaaagcta aagcaggtga aggaatgaaa gtaaaagtaa aaaaaagagc
     9481 aagcaatcga ttgaaaaaag gatatccact gatccaaaaa gaagaccttg ctcatcccca
     9541 aaaagaaacc acgcaatggg tggaattagt tgattcgcaa aaccaattta tcgcaaaagg
     9601 atatctagga aagcagaata agggaatcgg ttgggtattg agccagcaga acatcccatt
     9661 tgatcaatct ttttttgaag aattttttgt gaagtcaaaa gaaaaaagaa ctttcttttt
     9721 ttcagatgat tctactaccg cttttcgtat attcaatgga gaaggagacg gaatcggcgg
     9781 tctgattgtt gattattatg caggttatgc tgttttttct tggtataacg aaacgttata
     9841 tgcacatcgt gaattattga ttcaagcttt tcgtgaaatc tacccggaaa tcgtaggagg
     9901 ctatgagaaa attcgatttg aatcagtaaa tctgcctgag agccagttca tttacggaaa
     9961 agaggcaccg gaacccttga tcgtaaaaga aaacggagta aaatttgccg tttatttaaa
    10021 tgaaggatta atgactggga tctttttaga ccaaaaagaa gtacggggat cattagcaga
    10081 aggtttcgct tctgggaaaa cagttttgaa catgtttagt tataccggcg ccttttctgt
    10141 tgctgccgca atgggagggg caatcgaaac aacaagtgtc gatctagcaa aacgcagttt
    10201 aaaaaaaaca caggaacagt ttgaagtgaa tggtttggat gtcgatcagc aaaaaatcat
    10261 tgttatggac gtattcgagt atttcaaata cgccaaaaga aaacaatttt tatatgacat
    10321 gattgtattg gatccaccaa gttttgccag aaataagaaa aaagtctttc gcgtagcaaa
    10381 agattatggg gaactggtca aggatagtct ggatatttta gcagaagacg ggatacttat
    10441 tgcgtctaca aatgctgcga atgtctcgat agaacaattc caaacaatga ttgaagagga
    10501 attccatgcc aaaaatgttt cttttgaaca acttgcttta tatcgtctgc caaaagattt
    10561 ccagacgacg gatacctttt tagaaggcaa ttatctgaaa gtgtttgttt atcaaattaa
    10621 aaataaccat tagaaggagc gatgcagaat ggtttgggta aagggattga atataggaag
    10681 cggtagacca aagatttgtg cgcctcttat cggggccgtc cgagaggaag tattacgtga
    10741 agcagttctt gcacgtgaag caggggccga tttagtcgaa tggcgagtcg atcattatcg
    10801 tgaagttttt tcttttgatg aagtagcaga aacattggtt cttatccatg agatgcttga
    10861 tggactgccg ttattattta cgtttcgtac cttagctgaa ggcggagaac aagacatatc
    10921 gatccgtcag tatcgcgacc tttatgaaca tgtgatacac acaggattag tagatatggt
    10981 cgatatcgag ttgttcaaag tagaaagtct aggaaaaaga ctgatagagg aaatcaagtc
    11041 tttaaacatt cctttaatta tcagtagtca tgattttaaa gaaacaccag cagaccctgt
    11101 cttgctttac cgattgaatg ttatggaaca ttttggagcc gatatcggaa aactggcagt
    11161 aactcccaat aatgaacgag atgttttgcg gttgatggag ttgactagac gggcaaatgc
    11221 ttttgtctcc atgcctatta ttacgatgtc catgggaaat cttggtaaaa tttctcgtct
    11281 ggctggcgga acgacgggtt ccgttatgac atttggttcc ttaagcagcg attctacttc
    11341 tgctccagga caattaccgg tcaaagcatt gaagcaaatt attcaattat taaatgaacc
    11401 gttgtagaac aagtttgagg tgatattttt tggcaaagaa aaaaatagta aaaacgaatg
    11461 caatccgtat ggtcgaacaa aaaaagattc cctatacaga gcacgaatat gagtgggacg
    11521 aaagccacct aagtgcgtca tcggtcgctg aacagcttcc agaaagccaa tctcgtatat
    11581 ttaaaacact ggttgctgtg ggcaatgtga cggggcctct tgttgctgtg ataccgggag
    11641 aagccgaatt gaatctaaaa aaattagcaa aagtttcagg gaataaaaaa gtagaaatgc
    11701 tccatttaaa agatttggaa gcaacgaccg gatacatacg tggtggctgc tcgcctatag
    11761 gtatgaaaaa actatttcct acttatttag atcaaattgc agaaagctat gaacaaatca
    11821 ttgtctcggc tggcagaaga gggctacaga tggagttagc acctcaagat atctgtgcat
    11881 taacgagcgg acaatttgca gatataaaac aataaatcat acgtataaaa gaaagaagga
    11941 ggccgggatg ttgtttgtcc agtctccttc tttctttttt atacattatg ctctttgccc
    12001 ggcattcatt cctgctacat gccctgtagc gaaagccgct gtgatattaa agccgccagt
    12061 ataaccattg acatccagta attctcccgc aaaatacaga ccatcaatga ttttactctc
    12121 caatgtttta ggataaattt cctttagaga aacccctccg ccagttacaa atgatttctc
    12181 aataggaaaa gttttgttga ttgatagctt aaactctttg catagttgaa caaaatcttg
    12241 aatttgtttt tgagaggctt gttgtcctgt cagatgatcc atttcaagcc gctcaagaaa
    12301 gaaaactaac aatcgctcag gcaatatacc atgccaagca ttgaccaagt tctttttggt
    12361 tgctttactt ttttctgtca atatatgaat aagctcttgt ttggtttgtg tagggaaaca
    12421 atccagagaa agtgtcactg gttctcccgt tttctctaat tctttattga caaaacttga
    12481 acaacgcagt gcagccggac cagaaatacc gaaatgggtg aaaagtaaat ccatcgtatg
    12541 ttcagtcaat acacgacctt ttttatttaa aacgcgtaaa gtgatgtctt gtaaagaaag
    12601 cccttgaagt gttttctcct gaatatacgg ttcatccgat atcaaggggg actctgtcgc
    12661 ataaagaggc gtcaccgtgt gacccactcg cttcacgatc ttgtacccat ctccagtaga
    12721 accagtagat ggataagtcc gtccaccagt tgttaagata atggcaggag catggaactc
    12781 ttcgaattcc gtgcgtatac cgtaaatttt atgttctttg tggatcaatt tctctactcg
    12841 agtagagaat agaagcgtca catctagttc tttcagacga ttgagcaatg cttcgataat
    12901 cgtttttgat ttatttgtta caggaaacat ccgtccatga tcctcttctt ttaaatgtac
    12961 gccttgtgat tcaaagaagt tcatgatatc gaaattattc cattgcgcaa acgtactata
    13021 taaaaacttt ccatttccag gaatatgtgc aatcaaatca tcaacaggac gattgttcgt
    13081 aacattgcac cgaccaccac ctgtcatcag tagttttttt cctgcttttt tgtttttttc
    13141 gatcaagagt acgcgtgcgc cttgttcagc tgcactgatc gctgccatca tcccgcttgt
    13201 tccagcgccg actacgatca catcatacag tttttccatt ccctcacctc aaggaaagtg
    13261 tagcatatct gaagaaagaa aacagaaaaa aaatgttttg agccaaaaga gaaaatgagc
    13321 taaaatgagg gaagataagc aaaggagcgt ggaacgatga caaacgcaac agaatatgtt
    13381 caagcaattc aagaaaagct acatcaaaag gatcaagggc aagaagaatt tttacaagca
    13441 atcgatgaat tcatgcccac ggttatgtct tttttggaca ctcatcccga atatatcgaa
    13501 aagaatatct tagaactttt aacggaacca gaaagagtta ttcaatttcg agtgccttgg
    13561 caagatgatg aaggaaactg gcgagtaaat agagggtacc gtatccaata caactcagct
    13621 atcggccctt ataaaggtgg attgcgcttc catcctagtg tcaatctaag tatcttgaaa
    13681 tttttggctt ttgaacagat tttcaagaat agtttaaccg gtctgccaat cggtggagga
    13741 aaaggcggaa gtgacttcga tcctaaagga aaatctgata atgagatcat gcgtttttgt
    13801 caaagtttca tgctggaatt gtcaaaacat atcggcccat cgattgatgt tcctgcggga
    13861 gatatcggtg taggagctag agaaatcggc tatttgttcg gcgaatataa acgattgaaa
    13921 aaatatgata ctggtgtgtt gacaggtaaa cctttggatt tttggggaag taagatacgt
    13981 acggaagcta ctggttatgg attagtgtat tatgtcaaac atctattgaa cgaagaaaaa
    14041 gattcattta atcaaaaaac agtttttgtt tccggaagcg ggaatgtagc catttatgca
    14101 atcgaaaaag tgcaagagct aggcgggaaa gttgttacat gttcagattc tagcgggtac
    14161 atctatgatc cagaaggaat agatgtgaaa ctattgaaag aagtcaaaga aatcaataga
    14221 caacgtttga cggaatacgc aaaccaacgt ccttctgcta tttatcatgc tggagaatct
    14281 gtttggacat tgaaagaaaa agcggatatc gctttgccat gtgctactca aaatgaaata
    14341 gacgaaagct tggccgaaat cttagtagaa aatgggatca aaatcgtagc agaaggcgct
    14401 aatatgccat gtacagtaaa agctgtttct gttcttcatg aagcaggcgt gtggtattgt
    14461 ccaggaaaag ctgctaatgc tggaggagta gcagtatctg ctttagaaat gagtcagaac
    14521 gtccagcgat tgacttggga aagagagcaa gtggatgagc agctggatca aatcatggaa
    14581 acgatttacc atacgtgtcg ccaaacagca gccgagtacg gcgacgaaaa aaatctgctt
    14641 cttggagcaa atgttgctgg atttgaaaaa gttgccaaag caatggcagc agaaggttta
    14701 gtttgaaaaa tgaaactttc ttggaaatat tgtcagcatt ttgtttattt gcttgaaaaa
    14761 taataaaaaa aaacgtaaag tattacctgt agagtaaacc tataaggagg agaacaaatg
    14821 gcacacattc attttgacta ttcaaaagta gcaccgtttg tcaacgaaca agagttaggg
    14881 tacatgcaaa gccaagtaac agcagcccac aacgaattac gggaaggtac tggcgcagga
    14941 agcgacttcc gcggctggat cgatttacca acgaactatg acaaagaaga atttgcacgt
    15001 atcaaagaag cagctaaaaa aatccaatct gattctgaag tattagttgt tatcggtatc
    15061 ggtggttctt atttaggcgc tcgtgcagcg atcgacttct tgaatcacac attcttcaat
    15121 ttattagata gcaacacaag aaaagcacca caaatcttct ttgccggaaa ttcaatcagc
    15181 tcaacatata ttgctgactt gatcgaagta atcggtgatc gcgacttttc agtaaacgtt
    15241 atttcaaaat ctggaacaac tactgaacca gcgattgcgt tccgtgtatt caaagagctt
    15301 cttgttaaga aatatggaca agaagaagca aacaaacgta tctacgctac aacagataaa
    15361 gcaaaaggtg cagttaaagt agaagcagat gcagaaggtt gggaaacttt cgtgattcct
    15421 gatgacatcg gcggacgttt ctctgtatta actgctgttg gtttgttacc aatcgctgtt
    15481 agtggtgcag atatcgatgc attgatgcaa ggtgccgctg atgcaagcaa agcatactca
    15541 agcgacaaac tagaagaaaa cgaagcttac caatacgcag caatgcgcaa cattctttac
    15601 cgtaaaggca aagtaactga gttgttgatc aactatgaac caggaatgca atacttctca
    15661 gaatggtgga aacaattgta tggtgaatct gaaggaaaag accaaaaagg catctatcct
    15721 tcaagcgcaa acttctcaac tgatttgcac tctttaggac aattcatcca agaaggacgt
    15781 cgcaatattt ttgaaacagt tgtgaaggtt gaaaaacctc gtaaatcaat cacaattcct
    15841 gaacaagcag aagatttaga cggtctaggc tacttgcaag gaaaagaagt ggacttcgta
    15901 aatacaaaag catttgaagg aacattgctt gctcatacag acggtgacgt gcctaacttg
    15961 ctagtcaaaa ttccagaaat ggatgcttat acattgggtt atacaatgta cttctttgaa
    16021 attgctgtag gtatttcagg ttacttgaat ggaatcaacc cattcgacca accaggtgta
    16081 gaagcttaca agaaaaacat gttcgcactt ctaggcaaac caggctttga agatcttgct
    16141 aaagaattga acgaacgcct gtaagataag ggtttttcgc tagatttaaa taatcagtga
    16201 tagaaacatg ttcgctttaa aatgaaaact ggtacccatt taggtactca aacatactgc
    16261 atcgtttatt cagaataagg ggctgtgaca agacgtttgt cacagtccct tgtttgtata
    16321 gaaatcgatt gattgccttg ctctttagtt tgttttagga ctttaagaac agtgaggctg
    16381 acttcgaagg ttaattctgt ttttttgttt tctagaatca gtaagcaaaa aagcactcac
    16441 ttcgagtgct tttttgcgat atattcttag cttaagatag gcctaatttt tcttttacag
    16501 atgagggtac cttatcgctg gtaattactt ttttctctat gatccgcttt tcgcttgctt
    16561 tcaactgcaa gatcgttcct tcggggtaag cagaaccatt ggcagagtca ccagaagtaa
    16621 tgacatcgaa atctaactgt cttttttcac cgttttcagc ataagcatct acgttgtatt
    16681 taaaacctct tccaattact tccccattat ctgctttgat atcagtttct gcaggcattg
    16741 gagctttgac taccgcataa taatctttac ctacataagt agactgatag tagtcccaac
    16801 ctttccatcc tataaaagca atcaatatga taacgatcaa ccctaaaatt tttttcatga
    16861 aagatctcct tttttatttt tattataccc gtggtgctag ttaattttaa tatcctaaag
    16921 aatatctcaa agttccttca aaacgtcgtt gtgctcgact taatagaaca ctgtaagcca
    16981 gtaatatgct ttcaaatcta ctctctagcc ctttaacaga acgtgagttg aaattttgac
    17041 aacctaattt ttctaaggaa gaaaaaacag tctcaactgt t
//
LOCUS       DACDTO010000060        16406 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDTO010000060 DACDTO010000000
VERSION     DACDTO010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.060"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1141
                     /locus_tag="IWK70_001959"
     CDS             <1..1141
                     /locus_tag="IWK70_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9614478.1"
                     /translation="RNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVLDFVARTMSTLE
                     VKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIFTDDNQLLIADD
                     FSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTRGLFEDYAALFG
                     RILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIWNSFKTNSVAIV
                     PKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALIYGEKAELDSNI
                     KAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLEYATQIDKITSG
                     GAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            1128..1832
                     /locus_tag="IWK70_001960"
     CDS             1128..1832
                     /locus_tag="IWK70_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9614479.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            1837..2997
                     /locus_tag="IWK70_001961"
     CDS             1837..2997
                     /locus_tag="IWK70_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9614480.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            3073..3351
                     /locus_tag="IWK70_001962"
     CDS             3073..3351
                     /locus_tag="IWK70_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9614481.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            3332..3703
                     /locus_tag="IWK70_001963"
     CDS             3332..3703
                     /locus_tag="IWK70_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9614482.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            3719..4123
                     /locus_tag="IWK70_001964"
     CDS             3719..4123
                     /locus_tag="IWK70_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614483.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            4120..4530
                     /locus_tag="IWK70_001965"
     CDS             4120..4530
                     /locus_tag="IWK70_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614484.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            4591..5178
                     /locus_tag="IWK70_001966"
     CDS             4591..5178
                     /locus_tag="IWK70_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9614485.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            5251..5718
                     /locus_tag="IWK70_001967"
     CDS             5251..5718
                     /locus_tag="IWK70_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP9614486.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            5764..6072
                     /locus_tag="IWK70_001968"
     CDS             5764..6072
                     /locus_tag="IWK70_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614487.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            6267..13001
                     /locus_tag="IWK70_001969"
     CDS             6267..13001
                     /locus_tag="IWK70_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9614488.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            13005..13877
                     /locus_tag="IWK70_001970"
     CDS             13005..13877
                     /locus_tag="IWK70_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614489.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            13874..14501
                     /locus_tag="IWK70_001971"
                     /pseudo
     CDS             13874..14501
                     /locus_tag="IWK70_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14462..14923
                     /locus_tag="IWK70_001972"
     CDS             14462..14923
                     /locus_tag="IWK70_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614490.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            14927..15751
                     /locus_tag="IWK70_001973"
     CDS             14927..15751
                     /locus_tag="IWK70_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614491.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            15769..16314
                     /locus_tag="IWK70_001974"
                     /pseudo
     CDS             15769..16314
                     /locus_tag="IWK70_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         5866 a         2530 c         3510 g         4500 t
ORIGIN      
        1 aagaaatgaa gagccttctg agtggatacc ggattttgta tatggagagg aagtatctgc
       61 tcgggcatac ctcaaaatta tggcaaaaaa tactgtttta gattttgttg caagaactat
      121 gtcaacgtta gaagttaagt ttaaaaataa agatgggaca tcagactggg attatattct
      181 aaatgttcga cctaattctg atatgtcagc agctacattt tggcaaaaat ttttttatcg
      241 gctcttagat gacaacgaag tgttagtaat atttacagat gataatcaat tgctgattgc
      301 tgatgatttt agtcgaacgg agtatgccgt gtatgatgat gtttttacaa atgtgatggt
      361 aaaaaatttt gtctttgaac gttcatggaa catgtctgac gtcatttata tggaatataa
      421 taacgaagaa ttagaaaggt ttacacgagg tttatttgaa gattacgccg cgttgttcgg
      481 acggattcta gaagttgcaa tgcgtaacaa ccaaattcgt gggtctgtat ccattgattc
      541 aacgggaacg gcgaatgatg aaaaaggaaa agatggtaag actagatctc aaagattaca
      601 agaatatatt gataggattt ggaactcttt taaaactaac tcagttgcaa ttgttcccaa
      661 agtgaaaggc tttgagtatg aggaatacac caataaacaa ggcgtatcga atcaatcgct
      721 agatgaactg aataagatga agtcgtcttt aattgatgat gttgccaacg ccataggagt
      781 acctacggcg cttatttatg gtgaaaaagc tgaactggat tcaaatatta aagcttttcg
      841 taaattgtgt attgctccac tagttaaaaa acttcaagac gaattggtgg caaaaataat
      901 tagtcgtcaa gagtacaaaa acggggaacg tatagaagtt aaaaaagttc tccaaaaaga
      961 tctattagag tatgctactc aaattgataa aataacatct ggtggtgcgt tttatattga
     1021 cgaagttcgt gatgaagttg attatgatga gttgccaaat aatgaaggaa aaattcgtat
     1081 tcgtacgaaa aactatgaag aaactgtgaa gggaggtgag aacgacaatg ccgaaagcta
     1141 aaaaagtacc atttcagttt actaacgaaa ttcagaatgg taagcacatt ctcaccttga
     1201 gtggaaatgt acagaaaaaa tattggcgtg atgatgatgt cataaacgct aaagatattc
     1261 gtgactctct agatactgta accgatgata ttgtaatcaa gttgaacagc ccaggcggtg
     1321 atgtgtttga agggattgag atttacaatt atttaaaaga tcacccatca aatattactg
     1381 tagaagttac agggcttgcc gcaagcgcag caacgtttat tgtcgctggt gccaatgatg
     1441 tgattatgaa tgttgggact tctttgatga ttcatgaagc ttctacattt gcttggggga
     1501 ataaacagga catccaaaaa acgttaaacg cattggaaac gatcgatgat tctatcttag
     1561 ctatttattc agataagact ggccaatctg ttgataagtt gcgtgaatgg atgaatgaag
     1621 aaaaatggtt tacagcagat gaagctgtca aatatggatt tgccaattct gtgaagcgtg
     1681 aagaaccaca aaatgaacct caaaatattg cagcgatgat tcaagatgct gttgccgttg
     1741 caatagcgaa tttaaaggaa cctgttacta ataaaatcga acaagaatca aaacaaaaat
     1801 cattaatcgc acgattgcga aaaggagaat aaagatatgt taaaaattac agataaaact
     1861 gcagatgcaa aaaaggcatt taatgctatt tcttcgaaag aagatgcaac acccgaacaa
     1921 attaatgctg ctctagaaga atatgtaacc gccattgcag aagatgccgg caaacaggtg
     1981 cgtgctgaat atgaagaatt gaaaaatgtg acggacaacc gtgtattaga ggctcgtgga
     2041 attcctacac taacagcaga agaaacaaag ttttataatg aagccgtaaa atctggaggt
     2101 ttcgattctg acgtagtttg gccagaaacg attttggaac gtgtttttga aaatcttcaa
     2161 aacgatcatc ctattttagg cctcattaac tttacaccaa ctgtaggtcg tgtcaaagta
     2221 attagagcac gtcgtaaagg tgttgctgtt tttggaccat tgcataaaga ccttgaaggc
     2281 caactagatg ctgaatttgg tgcaactgaa tttgttcaac ttgcgttgac cgcatttttc
     2341 ttaatttcta atgatacctt agatttagga ccacgatgga ttgaccgttt tatcaatctt
     2401 tctttaagtg aagcagtacg tgatgtttgg gctaaaaaaa ttattacagg ttctggtaaa
     2461 gatgagccaa ttggattgct taaagatcta gatggtgcgg tcacaagtgg tgtatatcca
     2521 gataaagcta gcgcagggac attaactttt gcgaaagaga agattgttct tgaacttgca
     2581 ggggtgatga aaaaattatc taaatacaca tacaaagtag atggtgacga tgaaggtgaa
     2641 gtgaaatacc gttctgttgc tggacgagtg tatctaattg ttaatcctgt tgattactgg
     2701 gatatcgtag ctagagttac atttgcaaat ttaaacaatg tgtatgggac tacgatgcca
     2761 tttatcgctg ttgatcatat catcgaatct gtagacgtac cagaaagtaa atgtattgca
     2821 tacgttgata aagagtatga agctactcaa tcaagagctg aaaaagtcta tgtatataaa
     2881 gaaacttttg caatgaaacg cgcaactttg tatgctgtcg acatgcttgg taatggctat
     2941 ccaacaaata atgatgcagc acaagtatat gacttggact ttacagcggg ggagtaatgt
     3001 cccctgcaat tggtgagata acaccgacga ctgatggggc agtcattaat ctttcgtaaa
     3061 tggggttaga gtatgaacac tgaagaattt ataaaagaat ataaggcccg atttcgtatt
     3121 ttccatactt ccgaagatga ggatatcggg aaacagttag agagcgggtt tgccgatata
     3181 aaatctctaa tcggcagttt tgatccttcc acttatgaga aaggaaaaga attagtttat
     3241 gaacgtactc ggtatctacg gaacgaatca ctagagtact tttacgacaa ttttcaaaca
     3301 atgattatgg atgcatcaat tgatctggca ggtgctggat atgccaatta aatcaaatta
     3361 taagaagcct aaaattgtag ctggtgatct aaatacgcca gttacttttt ttgagtttca
     3421 acctaataag ggtccagaac ctggtgatga agaaaaaaag gaactgtatt tttgtacagc
     3481 actagtttac aacccatcaa tgaaagatcg tgacattctt ggcacaaaag aaggcgttac
     3541 gataaaaata cgggatcccc atcaatctta tattcctact aacaaacata aagtaaggat
     3601 attagattat cgatatggag ataaagtttg ggaaataatc gatgtctcac cggatttgga
     3661 agaaaattca attgtaaaga tagtattaga attaattact taaatttggt gataaatgat
     3721 gagtaacgta aagtttgaag gcgttcaaga aacaatccaa gctttagaaa aaaagtatgg
     3781 agaaaagaaa actaagacat taacaaaaaa agcaatcaat gttggcgctg aaaaagtaga
     3841 aaaacagttg caaacggaca tgaccgtttt taaagacaaa ggttatacga tcgctgaggt
     3901 tgttcgcaag aacgctacgt atagagatta caaggcagaa gctgaaatcg gttggaatgg
     3961 tccacatcaa cgatatagaa tcattcattt gaatgaatgg ggatatacac gaaagggcaa
     4021 acaaattaga ccgagaggat ttggtgtaat aacaaaatct cttaagaatt ccgagctgtt
     4081 gtactttgat gctgtaggag aggaggttaa aaagagttta tgaaagatat gcttaatatc
     4141 atttacgagg ctctttgttt gaatgagtat attcattcaa tgacctacaa cgagaatacc
     4201 gagcaatatc gtattaaatt ttatgagcaa cctgaaaccg ccgataaaac aggtccaatg
     4261 atcacaattc gtcctgtaga tacacctaac gaagcgtacc acggaagtaa caaagagctt
     4321 tctgtggagc atatgataca gattgacgtt gaatcgaaat atagagcaac atgcaagcag
     4381 attcaatatg agataaaaaa agagatgaaa aaactaggct ttggccaaat ttccggtcaa
     4441 gggttagatg aatactttac tgaaacaaac cgctttgtag acgcgcgtcg ttacgaagga
     4501 aatacaaaaa tttacgatac cgaatattag aaattaagac acgaaaataa cgtgtctttt
     4561 tttattaccc aaaattagga ggaaaatttt atgactttag taggattcaa aaaaatgaca
     4621 atcggggtct ttgacagcac agggaagatt ccagcagcaa atttatatgt aattgaaggt
     4681 gaacaaaaca aaggggcgac tgtatctgct gaaatcagtg ggctatcaaa agaaccatca
     4741 aaagtttacg gatcgaacat cgcttactat gtctctcaaa aaggaacagg cgatgtttca
     4801 gctacttttg gattgttaga tttaccaaca gaagtaaatg acaaaattct tggttacaaa
     4861 accgatacaa acaaaatcag cttcttggga gaagatactg agccaccata ttgtgcaatt
     4921 ttgatggaat cggaggattt gaacggcgat acagcaatgc ttgcgatgtt caaaggaaaa
     4981 ttcagtcgtg aatctattaa tcttaacact acgacaaatg aagctttcga accagaagca
     5041 gaagaatatg tattttcagc gattgccaat gatgttgaag gtgatgctaa aggccaaaca
     5101 gtcgcaaaat atgttggaga cgaagaagct tctattactg gtttgaaaga gatggtattt
     5161 cctgcagcgg gggagtaact agccccgttg ttggagcaat cactcccact attaatgggg
     5221 caacagttga attaagttag gaggataact atgccagata catttaaaat ctataaaaaa
     5281 aatggtactg aatttacaaa agtcgcagag ggtgaaagtc cactttctat tactggaatt
     5341 gcagcaaata ctcaagttgc taaaggagat tatcaagcta cgcgtgtggt cggaggtcaa
     5401 gaatcagtaa aagtagatat tcctgcattc accactcttc caatttcagt tactggtgtg
     5461 acgttggata aaacgacagc tgaagttgaa caaggcggaa ccttaaaatt aactcctact
     5521 gtaacaccag caaatgcaac agacaaaaca ggaagctgga gcagttcgaa tacagctgta
     5581 gctacggtta ctggaggcaa cgttacagta aaaacagacg ctgaagttgg cggaactaca
     5641 gaagtttcat ttactactac ggatggcggt aaagtagcaa aatgtactat tacagttatt
     5701 gaaaaagcag cgggctagtc ttcggactag cttttttatt tggaaaataa tggaggaatc
     5761 taaatggcaa aagtaagaat tgaattgaaa gaagaatcag gaaagaaagt ctatgaaaac
     5821 atagacacaa cagggaaaga ttatcgaaaa gctttagaaa caatcaaaaa gctaaatgaa
     5881 gacggagtaa tgatttggaa tcgtttagac atttatttgg attttgctgt aggtatcttc
     5941 agggatagca aattgacagc tgaacaaatt cttgatggct taccatctga gaaggtaatg
     6001 agcacattgg acgacatcct aggagaagta atggggattg aaagtaatcc agacccagac
     6061 gcaaaaaagt aacacccgaa gaagcagaag aaatgtactt aaacctatgc agagagttag
     6121 tgaaagcggg atggtcacta tctgacatag aagataattc ctttgaaatg atgatgaaaa
     6181 ttgcgtgcac ttcgcctaag aaagagaaac aaaaagaagt tgatcttaaa gacttcttga
     6241 aatccatgta gagaggaggt aaacatatgg caaatggaaa acctttaggc aatatgatta
     6301 tcaaactaga cttagacagt tcagcttttt caaaaggtct agccggagct aaaaacgctg
     6361 ttaaccatca aatgaaagca atgaaatcac agatgcaagt aatgaactct tcaggaaatt
     6421 ctctcggtgc tttacaagca aaatacaatg gtttaggcgg agttctacaa gcaaatgaaa
     6481 agcaagtaga attgctcact aaggcttata aggatagttt tgactctaac ggaaacgcta
     6541 ctgcttcgac tgctaaatac gctaatcaat tgaatcaagc aacagctaga tcagcaagtt
     6601 acgaatctca gatgaaaaca actgttggac agattgctcg gatgaaagtg gaaacggaag
     6661 ggtttacggg gaaactaaag gctaattctg atgtattgat taattctggg aaaaaaattg
     6721 aagcctttgg taaaggagtt tctgggatag gatctgccct aactgttgga gtaacagctc
     6781 caatagtggc aggcgcaact gctgttacta gtgcagctat ttcatgggaa agtgcttttg
     6841 caggtgtaaa aaagacaaat gatgaagttg tagactcgaa tggaaacgta gtttattcct
     6901 atagcgactt agagaaaggt ctgcgtaatc ttgccaccca attacctggt agccatcaag
     6961 aaatagcaaa tgtagccgaa gcagccgggc aattaggtat aaagactgaa aacgtagtta
     7021 gcttcactaa aacgatgata gacatgggtg aatcgactaa tatgtcggct gaaactgcgg
     7081 caacttcttt agcacgattt gctaatatta cccaaatgtc tcaaaaagac tttgataaat
     7141 taggatcagt cattgttgat ctaggtaaca attttgctac aaccgaatca gaaatcactg
     7201 aaatgggatt gcggttagcg ggagctggta aacagattgg aatgagccaa ggcgaaattc
     7261 taggatttgc tactgctcta tcttctgttg gtgtcgaagc tgaagcgggt gggtctgcct
     7321 tttcgaaggt catgatccag atgcaactag ctgtagaaaa aggatttggc gcttttgatc
     7381 agctgaaaca gatggcagaa gaacaaggtg ttccgtgggt taatttagta aatgctgtga
     7441 gagacggtgg aaaatcgtta aaagcagtat cagagcaaat gggattcact tcatctgatt
     7501 taaagaaaat gtataaagaa gcggacaatt cgaaaagttc gcttgaaaat tttgcgaatg
     7561 tggcaggtgt tacaagtgaa caattccaga agttgttcaa gagtgatcct tcagaagcaa
     7621 tcatcaagtt cattcaaggg ctgaaagatt cagaaaaaca tggtacttca gcaatcaaag
     7681 ttctggatga catggatata aaagaagttc gactacgcga cagtttattg agagctgcaa
     7741 atgctagtga catattcaaa ggagcaattg aaaaaggaaa cggcgcgtgg aaacaaaata
     7801 cagctttaac aaatgaagct aataaaagat acgaaacaac tgaatcaaag ctaaagatat
     7861 tgaaaaacga agtgattgat acagcaatcg atcttggagg tccatttgta gacgcgttgc
     7921 gtgatggtct aaatgtatcg aaaccattga taaaaaactt agggaatatg gcgaaagctt
     7981 tctcagctat gagtgatgat caacaaagaa acattatcaa atgggttgga ttagcagcag
     8041 cagctggtcc agcgctgaag cttttaggaa ctggtacttc agtaataggt aaagttactt
     8101 caactactgg aagattgacg aaaagccttg ttgatttagc agcgaaagca gcagaaaaga
     8161 aggctatgga tgcctttgca tcgtcggtaa caacagctgg tgctgcagct gcaaagacag
     8221 caggagctgg aggtattggc gggttagtcc ctatgatggg aaaaacagct agtgctgcaa
     8281 gtactgctgc gggcgccagc ggaataggcg caatgacaac gtcactaggt ttgttaggtc
     8341 ctgctctact tggtattgtt ggcgttggtg gcgcactggc agttggctat ggagcatgga
     8401 aattatttgg tgaagaagct tggaattcct cgcaaagagt aaagcaatgg ggaacagatg
     8461 tcggtgagga gatagatagt actcttgacg gagtacagaa gaatattgaa ggtgcaaatg
     8521 gtcaattcgg tcttctcaaa gagggtttta ctgttgatga tgcaaataat atggcggcta
     8581 atttcgcaac cgctggaaca tctttggaaa catctttaac gggtagaata aaggcattag
     8641 acgatgcact aaaagggcta cctcaatcag ttcaggatgc catgaaagaa gtagctgaat
     8701 cagaagaaga aaccttaggg caatctcttt ctaaaataaa agacaacaat gaagaaataa
     8761 aaaaaatcag agaaagagcc gcaaatcaga acagagactt gactgtttca gaactacaac
     8821 agatacagga tctaatgaaa ggaacttctc aagcgtatgt ggatacgctc gatgtttctg
     8881 ctaaagacag aaaaaagata ctatcagcca tgaatggtga tgttgcacaa gctacagaag
     8941 aagaagcgaa gacgtggtta caatctttag cgaaacaaaa agctgcaact accgaaaatt
     9001 atagacaaat gcttgaagaa aaaaaggcgg ctttaaagga aaagggagta agcaaagaat
     9061 tcattgatgc attagaagat gaaatgaatg attacatcaa aactacaaat caaggattcg
     9121 atagccaaat agctgctata acaaataaat atccacaatt gattaacgaa gtgagtttat
     9181 caaatggtca attatttagc aaaacggcag ctgctatgga caatactggt cagtatacaa
     9241 aagtttttct cgaaagtaat gaaaagattt tagcccaagc gaagaaaaca tcggatgaaa
     9301 tagcagataa tgccgaaaaa aatgcagaac aacttggtat ggtagctgat gaggctacgc
     9361 atgctggatc aacttggaat ggcttagttt tagacgaaaa aacaggaaaa gtaaaaacta
     9421 atgtacaaga agtagtgaat gaagcaacga aggatttcac taaatggaat gaattgaaac
     9481 ctgttgttca tgatgcgaaa ctaacaacga atgcaaaaga tgtcatttcc atagcggcag
     9541 tacaaaatgg ctattgggat gatatggaat ggaaagaaaa agaagcatta ttagaagatg
     9601 agtgttctaa aaatgtatac aaggctttag aaaatgctgg taaatggaat gagttagata
     9661 ttccttctaa agaagcaatc ctaacttcaa atactcctga agttatgggt gaaactctca
     9721 tgaatttggg attatgggat acttacaaac ttcaagtgaa agatttagat ttgaatgcgt
     9781 ataaagtata cgatgctatc gatggatcag aagaaaaatt aaatagttgg gctcaattag
     9841 ataaccccac aaaagaattg ttattagata actcagattt ttctacaaaa ctttttaatt
     9901 ccgaagaaat gtttaaacgt tggaatcagt tatcatctaa tgcaaagttt ctttcggcta
     9961 ataatactaa atttacagag gttcttttat catctgacag aatgtggaat caatgggtaa
    10021 accttcctga taatgagaaa agattacttg cagataacga agatttagct accactgttt
    10081 tatcttcaga agaaatatac ggaagatgga ctcaattacc agataatcaa aaatatatgt
    10141 tagctgacaa tactgactta gcaaacaaaa tattcgcttc agaagaatca tggaatgcat
    10201 ggaaagcaat tccagacaca gttaaaaact tgcttggtaa taatattgat ttactggcta
    10261 aagtgaaaga cgggactgta agtgtagaag aatataacaa agttatgcca cttcttaaaa
    10321 ctctatatgg agataacgtt aatttaaaag aggcaatcag tggggctaaa agctctatag
    10381 agaattacaa caaaaatatt tttcctagtg ataaaactgc agtaggacac gatgaggctt
    10441 cacaagctgc taaagatgca tttgaagcgt tcaacatctt ccaaacaaaa ataccagata
    10501 aaattactaa aactgtatca gctgacttta taggtcctat gccgaaaaat gcgaaaggaa
    10561 caaacttcca tcctggcgga gcagctatgg taaacgatca aaaagggcct acttatgaag
    10621 agcttataac cttgccaagc ggagaaactt ttatacctaa aggacgtaat gtcgttttag
    10681 acttaccaag aggttcgaaa gtactgaacg ccactaaaac taaacgtcta gtgcctaaat
    10741 atgctgatgg tataggaaac ataacgacgg tttcatctac gctaaactta gatgctttaa
    10801 ttttagctat taacgagcta acgacggtat tgagaactca actgatggca ggaacgactg
    10861 atgctaaaga tactaaacta tcagatgaaa caaaagttgc cgatccgatt attccggaca
    10921 gtctttctga aaaatcagat cagtatctag gaattggagc tcaatggtta actaatctaa
    10981 tgaatggttg gaactcagcg gttcctcaat atatgaatag tgaaatggtt tttattacaa
    11041 actatctcaa tgcgctaaaa cttcaaaaca atcctaatta tacgcaagga gcaacatgga
    11101 ataagaactt actgaatgga tggaatagtt taactgggac ctttattgct acaatcaatt
    11161 cattttgtaa tcaagcaatg gtcacgctta gaaactacaa cactcctatg tacaacaatg
    11221 gccgaacgtg gcagcaaaat aatcttaatg gttggaattc attgtatgga tcattcatag
    11281 cccgtgtaaa tcagcttggt aacgattcga ttaacaacct tcgttctaaa agtggtggtt
    11341 tttacaatgc agggacattc ttgttgcaat ctttaataaa cggaatgaat tcgttaggta
    11401 attctctttc tactacaatg aacaatgtcg ctaatacaat ggttggtggt ataggcaaag
    11461 gagttaatgg cgtcatatcg ggagttaact atgtgcttaa ggaagtcgaa tcaagtaaga
    11521 atatcggaaa ctggccaatt ccacaatacg ctaagggaac tgatggacat cctggtggac
    11581 ttgctatgat taatgatcaa aaaggcctag ttcacgaaga atatgttcag atgccagatg
    11641 gacgtagttt tattgccaaa ggtagagatc ttcttgttaa tttgcctaaa ggtgctcagg
    11701 tcttaaatgc ttctttaaca aagaaattaa aggaacgttt aaatattcca cactacgaaa
    11761 aaggtattgg caatttagat attgttgatt tgttagatga tgaaaaaaga atgttagaat
    11821 tcttaactag caaagttgat ttttcaggca ttaatgaacc atggcttgat atgactaaat
    11881 caggaacgtc attaatgtcc aaagctgcaa atacaatgct tcaatcaaaa ttaagtgaat
    11941 tcttcactca tggcaatttt gatggtgcag taaatgccaa tggtgtttat caatatttag
    12001 ttgatgtggc acagaaagta atgggtaagt tccccggact tacagtaact tctggatatc
    12061 gagcaggaga tgcctactat cacggaaaac ggcaagctat cgacttggct tatccaggta
    12121 tttctggaga tccgagatat acagcagcag caaattacgc ttttgaaaaa ttcccttcaa
    12181 aaattgcgta tgtcattacg aatggacgtg tacgtgaccg catgggatta tctggaacaa
    12241 ggtcaagcgg acaatggacg aattggccag atggcgatca cttcgatcat attcatttaa
    12301 acggttcgat ggggtctggc gatatattca atggaggagt tggaggaagt ggtgttgcac
    12361 gctggcgttc ttatgtgtct aaagcattga aaatgaatgg attaccagct acagcagcgt
    12421 atataaatgc ctggatgtcc caaatccaaa cggaatctgg tggtaatgag aaagcaatcg
    12481 gcggcaatga tgggctcgcc gaaggcaatg caacaggatt acttcaaacg aaacccggaa
    12541 cttttgcagc taacgctttt cctggtcacg gaaatattat gaacggattt gataacatgt
    12601 tagcagcaat caattatgct aagaaacgtt acggagttgc aggtatgttg caagttatcg
    12661 ggaaaggaca tggatatgcc aatggcggct tgattaccaa agacggacta tatcgagcag
    12721 gagaaggaaa taaaccagaa atggttattc cattgacaag aaaaacaaga gctatcgaac
    12781 ttatgggtca agcattagcc ttcctttctg gaagcaataa aaaagaaacg aaaagtgaga
    12841 ttaattctgt agataataga catatcctac aagcgataga aaaacagtct cgtatgacaa
    12901 atgatctgct atcattatta ctctcgtttt ttaaaagtaa caatcgatca gataaagaac
    12961 tagctttaga tattcagaaa attttaatca ggaggacata gtatttgagg acagtaatat
    13021 taagaaataa attgaatgaa gaaattgatt tgtccacaga agattatttt gctactgaaa
    13081 tgtcgaatct aggttttgaa gttacaaaag agcatattgg acaatggggg agtttcagag
    13141 aaataggaga gagtattgaa ttgtctgaat ttcaatcatc agttatgatt tctgttcacg
    13201 gatttagaga acaacaatta tatggttcat tagttgaatt tctggctaat ggaccttttg
    13261 aactagaatt ttcttttgat tcagaaacaa tgatcaggaa atgtagtttg aaatcattat
    13321 ctaaaacaga aattgattcc caaagctcat tgctaacaga tactttgaat ctatatttta
    13381 cttcaagctg gtattctatt aagcgtgaaa aattaataca gaattcaact tctctcaata
    13441 cacgtggaaa agtttactca tttgttcgac catatgtcta caccaaaaat gtatgggaaa
    13501 agaaaggtgt atttaagttc agtaataatt ctgtatattt gacaaatagt acagagcgaa
    13561 tgtctccgtt aaaaataagg attattggag agtgctcaaa tccatattgg gaagttatcc
    13621 aaaactctaa agtagtagct tcagatggct attttattaa tatgaaagac acacaaatat
    13681 tagaagtgtc tagtttgcta gaagaccaaa cggcaatttt aaaagatgta ggtggagttg
    13741 aatcttctgt ttatcaacaa caagatttta ctaaatcaaa cttcgtccaa gcgcctctag
    13801 gagaattcag cgttgttttt catgttggac atgcaaatgt agagattgaa ctttatgagg
    13861 agcgtgatct attttgattt tagccattac cctctttcac cgagatatga atttatataa
    13921 cgaacgttta atttcgactg agttcgaatt tggaacggat gaaattggag aagaagtaag
    13981 taattttatt acagataaat atgtccatgt aaaatcagga gattttctac tagcaaaatt
    14041 tattcctagc gggaagattg cttattttgg agtgattagc tcgcaagaag acaaaaaaat
    14101 aaattgcaaa ggattactca gtttggccga tagcgagata ccgactgtaa aaatatcagg
    14161 tgctaattat gaagagcata ttcgacgact gattgaatac tacttattga acgatccaac
    14221 aaaacaacta aaaaatattt tggaggtaaa aacagaaagc gttacttctc attcttatca
    14281 agctgttgac acaaataaga gacgattgaa tgcatatata ataaatgggt tcaaaaaata
    14341 taatgttaaa tggtatgtca aagagattaa aggtaaaaaa atatatactg gtattcgagc
    14401 agttgatgat tcaatttata ttaaagataa ttcatctgag tttaatgact gggatgtttt
    14461 tgtgcaatct cctggaccag ggaacgagaa caaattattg atcgtagata aagcaatgaa
    14521 agatatagaa aatcccttgg tattgtctac gtggtattta gatgacgaaa ataatcttac
    14581 gcaagattca acgaataaga acattgcatt gcctaccgtt aatttagtaa atatatatga
    14641 tcaaactcaa gaagatagac cttcttatga agaggtagct aaatcggagc ttaaaggcaa
    14701 cgcctattca catgaaatta aagttaatgt tgtgctaaat gctagaaata tcaatgtaga
    14761 aaatatcgaa acaggaatgt tagcgactat tgtttacaaa gagcagactt acaaatctgt
    14821 tctaactgcc tggcgaatat ccagcagtaa aaagaatata gaattgacat ttggtaatgt
    14881 ccgaagtcgt tttatggatt attttgaaga taatggggga taaaaaatgg ttagcaatgt
    14941 ggatggatat caatttgaaa acgtgaaagt aagcgcagaa aatgatgcta gactttatca
    15001 cgttttatat aatcggaaaa atcaggttat tgatggttac gatcagtcta tgaatttatc
    15061 ttcaagcgga ttaacagtaa aagttgctgc aggagcagcg attattcaag gtcgtatggt
    15121 cgttgttcga caagaagaaa gtataacagt tccagcaaac tcaagtggtt atatagcatt
    15181 aacagtggat ttgacacaag aagttatacc tggatctatt cttccagaat cggaagaata
    15241 tgaatggact aataatcaag tcaagctaga atttataaca aaagttataa aaggtaattt
    15301 aaacaatggt gataaggtct ataatttacc actatgctca gttaattcta ctggatcaac
    15361 tgtttcaatc tcaaagatat cggatagtta cgagctgact ctctctaaag gagaaatttt
    15421 gtggaggggg actgcgttaa tgcatgatac tcaaactgtc caaccttcaa aaaaaatttg
    15481 gcagacagtt tcaggttttt tgttagtatg gctcccatac gaaaacgggc aagcaattga
    15541 ggatagatat gtaactacgc ctttttataa ggagcgtata acttttacta atgttttagg
    15601 agaaattgtt tcaggatttg atgactatca caaaaagtgg tttagtaaac gaataaacta
    15661 taattcgaat actaatatat ttacaggtgc agcaagcaat gcaagtggag ataacgcaaa
    15721 tatggtgctt aggtatatag tttcttttta gttaggaggt atggaagagt ggcaaattta
    15781 gaaattaaat tatctgcaaa taagagacag ccttatctac gtcaccgtgt tgttggtaga
    15841 gttggtgatg gggggctcac aacaatcaat gtacaacttc ttgaggaaga tgaaattaca
    15901 ccttttgtaa ttaatctaaa cggtactttg aaatttgtgg gcgaagtttc aaacggtaac
    15961 tataccgagg gagaaccaga aataatcgat tcgactaatg ggttaattag ttacacgttc
    16021 actaagtcaa atttcagcac gagtcatcaa ttcaaacaag catattttga atatgtagat
    16081 cctaacggca aaaaagtaac ttttcagaac ttcatcatag acgtgttaga acgagtagat
    16141 attaattcgg agcaagcgaa atactatatt tcttcattgg aaaaattaca gagtgaaatg
    16201 caaaccactt tcaatcagtt cattagtgat aaacaggtcc aatacgatca aatctactcg
    16261 aaatataacg aattagtaag attgataaat gaatcagata agcaagtaaa tgatcgtatt
    16321 gataaggcaa ataatcgtat tgatcaaacc aatcagcaaa tcggcgatct cggcaagctg
    16381 aaaaagatgt acagtaacag cattga
//
LOCUS       DACDTO010000061        16210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDTO010000061 DACDTO010000000
VERSION     DACDTO010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.061"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            219..815
                     /locus_tag="IWK70_001975"
     CDS             219..815
                     /locus_tag="IWK70_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9614492.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(873..1355)
                     /locus_tag="IWK70_001976"
     CDS             complement(873..1355)
                     /locus_tag="IWK70_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614493.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1461..1658)
                     /locus_tag="IWK70_001977"
     CDS             complement(1461..1658)
                     /locus_tag="IWK70_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614494.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            1953..2711
                     /locus_tag="IWK70_001978"
     CDS             1953..2711
                     /locus_tag="IWK70_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9614495.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            2771..3199
                     /locus_tag="IWK70_001979"
     CDS             2771..3199
                     /locus_tag="IWK70_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614496.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            3245..3317
                     /locus_tag="IWK70_001980"
     tRNA            3245..3317
                     /locus_tag="IWK70_001980"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3278..3280,aa:Gln,seq:ctg)
     gene            complement(3467..3838)
                     /locus_tag="IWK70_001981"
     CDS             complement(3467..3838)
                     /locus_tag="IWK70_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9614497.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            4053..4238
                     /locus_tag="IWK70_001982"
     CDS             4053..4238
                     /locus_tag="IWK70_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614498.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(4321..4509)
                     /locus_tag="IWK70_001983"
     CDS             complement(4321..4509)
                     /locus_tag="IWK70_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614499.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(4533..4796)
                     /locus_tag="IWK70_001984"
     CDS             complement(4533..4796)
                     /locus_tag="IWK70_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614500.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            4954..5211
                     /locus_tag="IWK70_001985"
     CDS             4954..5211
                     /locus_tag="IWK70_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9614501.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(5282..5473)
                     /locus_tag="IWK70_001986"
     CDS             complement(5282..5473)
                     /locus_tag="IWK70_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614502.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(5614..5934)
                     /locus_tag="IWK70_001987"
     CDS             complement(5614..5934)
                     /locus_tag="IWK70_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614503.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(5957..6391)
                     /locus_tag="IWK70_001988"
     CDS             complement(5957..6391)
                     /locus_tag="IWK70_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9614504.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            6726..7364
                     /locus_tag="IWK70_001989"
     CDS             6726..7364
                     /locus_tag="IWK70_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9614505.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(7688..7771)
                     /locus_tag="IWK70_001990"
     tRNA            complement(7688..7771)
                     /locus_tag="IWK70_001990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7735..7737),aa:Leu,seq:cag)
     gene            8299..8799
                     /locus_tag="IWK70_001991"
     CDS             8299..8799
                     /locus_tag="IWK70_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9614506.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(8989..9333)
                     /locus_tag="IWK70_001992"
     CDS             complement(8989..9333)
                     /locus_tag="IWK70_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9614507.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(9487..10407)
                     /locus_tag="IWK70_001993"
     CDS             complement(9487..10407)
                     /locus_tag="IWK70_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9614508.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            10594..11085
                     /locus_tag="IWK70_001994"
     CDS             10594..11085
                     /locus_tag="IWK70_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9614509.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(11109..13484)
                     /locus_tag="IWK70_001995"
     CDS             complement(11109..13484)
                     /locus_tag="IWK70_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9614510.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            13810..14436
                     /locus_tag="IWK70_001996"
     CDS             13810..14436
                     /locus_tag="IWK70_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9614511.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            14459..15163
                     /locus_tag="IWK70_001997"
     CDS             14459..15163
                     /locus_tag="IWK70_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9614512.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            15355..16188
                     /locus_tag="IWK70_001998"
     CDS             15355..16188
                     /locus_tag="IWK70_001998"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9614513.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         4984 a         3151 c         2715 g         5360 t
ORIGIN      
        1 agctgttttt ttcaaataag caaaaattcc taaaacacaa caatgaaagc gataactaag
       61 aaaatcctta cataacgatt tttcttatta aaattgtttt ttgaacttcg cttatttttt
      121 gtgagcatat ctgtttactt actttttgta aggtgctata cttcatttcg taatcaatta
      181 aaaaaatgca aaacgcaaat caaaggagaa attttattat gacaactttt actgacactt
      241 tgaaaaaccg ccgttcaatc tatcatctag gacgtaatgt ttcactttct aatgaagaat
      301 taacaacttt gatcaaagaa gcaattaaag aaagcccaac tgctttcaat gctcaatcaa
      361 cacgcgcagt tatccttttc ggagatgcac atgaaaaact ttgggaaatc actgaagaag
      421 cacttcgccc attgactcca gcagaagctt tcccaaatac acaaaacaaa ttagctggtt
      481 tcaaaaatgg ttatggtaca gtattattct tcaaagatac tgacgtggta aaaggtctac
      541 aagaacaatt tgaattatat gcagacaact tcccagattg gtcagaacaa tcaaacggaa
      601 tcgcaacagc taatacttgg gtagcattag ttgataaagg attgggcgca aacttgcaac
      661 attacaaccc agttattgac gaagcagttg caaaagaatg gaatatccct tcaaactgga
      721 aactacgttc gcaattagta ttcggttcac cagaaacacc ggctggggaa aaagaataca
      781 tgaacgatgc cgatcgcttc cgcgtatttg gataatttta tttgaaataa aaaacagagg
      841 ttgtgacaaa catcttgtcg caacctctgt ttttattctg gatcaatcat aatatcaatc
      901 tctgcctcat cgtttaagcg ttgggacaaa aatggaacta attcttctaa tgatgcagaa
      961 aatttcagca tcggtcctcg ccatccgttt cggaaaaaat tcatttttac gattttagaa
     1021 ccttttggct gatataatac aattttattg atctcagaaa aaggaacacc ttttttgtca
     1081 aagctattca aaacaagtcc ttcttctgtg attcctctac tgtccaacaa aaaactaata
     1141 aaaatcattg ctgacaagat cccttttgcc aattcatctg ctgtaggatg actgatgagt
     1201 gtataccaaa tgactagtga cactaagaag ggtatcacca agtagaccca tcgatttcgc
     1261 acagtccgat aaataatttc ttttcgaacc agccaaagaa ataaaaacat cacaacaacg
     1321 aataaaaaca atagaaccgt aaagaatgtt accatgacaa ctcgcctcgc tttttcttct
     1381 ccagaaaata ggaaaagaat actgatttac cttctcaaag aaattggact tgcagcacgc
     1441 cgcacgtcca attgttttct ttattcaaat gtatcatatt ttgcaacaac catgcttgct
     1501 ttttcatcta ataattcttg gtcctctgca gcacattggg caactttgac cacggcgcag
     1561 tttgtcattt ttgatacaac ttcaccaatg accgtttttg taaaaccaat tggtttgcat
     1621 gcatattgtt ctcctaaatt aatcatttct tccgtcatca aaatcctcct aaactgctgg
     1681 aacttttctc cacattacca ctgctatttc tttggagatt taactcattg atttttattt
     1741 acagttaaaa atcaacctta caggcattat agcgtactgt tttcgttttc acaattaaaa
     1801 cagctgttgt atttagaaga atttttttca aaaaattaaa acatattcct taaaaactaa
     1861 ttatttccta catattaaaa tataatactt ccatttccaa taaaaataca tttataatag
     1921 gaaagataaa cagctgaaaa gggagatttc aaatgaagaa aaaaacgttc aaaaaactat
     1981 tgtttctagg gggcacagct gctcttttat taagcggtac aagttattac ctacatatcc
     2041 attctccaga acatacaatg aacactgcac aaaaaagttc aacacaaaaa acgaaaaaga
     2101 caaaagagaa agcagaagtt ttactagatg ttccattaga gagccagttt gattctcctt
     2161 ctttagaaaa tggctgtgaa gtcacttcgc tttctatgct tttatctttt tatggttatg
     2221 aaacaactaa aaaccagtta gctgagcaat tggattacgt ccctgttttc aatgcagatg
     2281 ggactcatgg cgatcctaat gaaggatttg ttggtgatat cagtggcgga gactgggcta
     2341 tggtcgtcta tgttccccca gttgcctccc ttgctcagca aatcgtccaa acggattatc
     2401 acacttttcc aaaaaccgat gctcaactcg atgatatcaa aaaagcacta tctaaaggaa
     2461 atcctgtctg gacttctgtg actatcaatt ttgaagtccc agatgaaact gattttatga
     2521 cttggaccac aaataatgga gaagtaagag tcactcctct tgttcatgct tgtgtagtca
     2581 ctggatatga caaagaaaat atttatgtca acgatcccta tggtgtaaaa aatcgagcag
     2641 taccgatcaa cgatttttcg gcgattttca ctgcaatggg tggacagatg ctaactttgg
     2701 agaaaagcta gtaattcaaa caaaaatccc ttatactcaa gataccaaac ctttgaaggg
     2761 agcgtagata atggaaaaat tcacacataa aaaaatggat ccaaatgaaa ttccgattat
     2821 ttttgtccgt gactgcaagg gaaatgttca aggtaaagtc tctattaatg agtggaacga
     2881 gcgtcgcaga cctgctaccc tcaatgaact ggaaatcaaa ttgtatcgtc aatctcttgt
     2941 ttattacgca gatcaagagt acgagaaagc caccgattta ttgaaatttc taattgctcg
     3001 aacagagtat actcgctttg aatatattga acgtttagca aacatctatc atattatgaa
     3061 tgaacctgtc aaagaatatc agttattgga caccgtcttg tcagtagcag aactgattgc
     3121 ccttcctgct ggattagaaa agaaactagt acgccgcctt ttacgagtaa agcaacaact
     3181 atccgatcaa gaaaaataac ttgcgttttt ttaggaaagt gctatgatta ttttgttgaa
     3241 taaatgggat gtagccaaac tggtaaggca cttgactctg aatcaagcaa ttgtaggttc
     3301 gaaccctgcc atcccaattt gatgtgcttg ctgaaaaaat gaaccccctg taaaagaaca
     3361 atctttttgc agggggatta ttgtattatt tttactactt gtatttagtt tacataatta
     3421 aattatgaat aataaaatcg ggcgtattct taatacaaaa actgatttac ccaataattg
     3481 ctaatgcttt ctccggaact ttttctctag gaacttcctc atacgtttct atgtgtgctc
     3541 ctttattgct aattttcaaa taatgatttt cttttaacac agaaagacca ttaaattcaa
     3601 ccgttcgttc tttcccattg gcatcagcag ctttctgcac ataccttgct gtaccatatt
     3661 catttacttc ctctggtttt tgtgtcttta catagacctc tcctttttca actaatggat
     3721 ttagctgatc aagtatacca gggatttctc cataacttcc ttcagtataa tatttcagtc
     3781 caaatagcaa aataccacta atcgccgcca gtccaatcaa atatttaatc actttcatct
     3841 ttctacctcc ttgttatata ttcagtatat gggtcagtta aagaaattcc atccgtccca
     3901 agttggagat ttttctccta ctttagaagc gagaatcgag tgttttttta gtaaaaatga
     3961 tcgttatttg atattattga cgtgtggata aatcagtttt tctcgttttg tccatgtaaa
     4021 aaatcatgtt gatagcaaag gatgtgttca aaatgaatca agaagaatta tttcaaaaag
     4081 tcaaggaaat gattaagaac ggcaatttcg atggcgcaaa acgatttatc gaagaacata
     4141 aagaacaatt aggaccttat aaagagaaag cccaaaatct tttaaaagat gtaaatatcg
     4201 acagtgtcaa aaacaaattt aaaaatctat tcaaataagt aaaactttca cttttgtaaa
     4261 atcaaaggag gacaatgctg ctgcatcgtc ctcctttggt tcttttctga atacttcata
     4321 ctaaaataaa cgtgtcggat cgtaatgata agtcacattg ccgttcagtg aggcttttcc
     4381 caccacatat ttacgattct ccaacttcag ccacgcttct cgaaaagcca ttaattcttc
     4441 tctttttaca tcaatctttt cgatttttcc atcgatcaaa tcttgaataa gttgttcata
     4501 tggattcatt tttttcctcc atctgctgtc aatcaataag ttgtaaaaat aggttcatat
     4561 gcttttttgg tatctagatc ttctctgact ttgactgtcg tataaataat tttttctact
     4621 gggatactat aataacttcc ttcaccatta ttgaatcggt gaacgtcttt ctttgctaaa
     4681 ataccattta ttttattcgt cagtacacgt tgttcgacac tgttacttac aacatagtaa
     4741 atcacccgaa tattattgat caaatgcaaa tcgatttcta acactacatg atccatgaaa
     4801 atcccactcc ttctttcact ttgattatag caagtttctc ctctaatttc aaaatcgata
     4861 cagattttac cggataaatc cacaaaactg cttgcgccat ttcgcacaaa gtgttatttt
     4921 gtatcatgga ctttatttat cggaggcaat agtatgatcg cgatcggtca atttgttttt
     4981 tatattcctt tttttattat gataagcatt cttttctatt acataaaatg gacaaaaaag
     5041 aagttttcag ttttactggc ctctttacct gctgtatatt ttacttatca aattttttct
     5101 tttaggcatt gggaaacaac gtctgtatta ataacacaca tcatcgaatt gacgctttca
     5161 gtaatttttc ttattatttg gatctatttt ttatataaga atcagaattg aatccctcgc
     5221 atgtttaaaa agaaaacgat taaagatgca gcaaatttct gccgatcttt aatcgttttt
     5281 tctattctgt tataccaaaa aaatgtttga atcggtgaag gatcgttcct tgttttaatt
     5341 ctataaattc ttgtgcgatc gggtactttt cttccaaatt ttctttgttc ttttcgatca
     5401 ggcttttcgc ttcttcatat tttttatctg cacatagtgc ttttacttcc tgtaatactt
     5461 ttcctgcttc catttgcctc accctcttgc cattttattg ctttaagttt tatttaagtt
     5521 atacgcatta ttctagtttc atcccaaata accttttttc atttttactc ctcgaactaa
     5581 tcagttttct aaaggctgat tggttcgtct tttttatttt tcctccattt cattctgctc
     5641 ttctgttaat ttatctaatg ctttgattga tgctgcggct cttgcataat catagctgta
     5701 taattccata aatttatcag gcagaacaaa ccacgctgga tgataaccgt tagctgtgac
     5761 agggaatcct ttatcttgtt ggttatctcc atcccatctc agagcaacac ctttttttcc
     5821 tttccacttt actaaagcga tcgagtatcc cttctttgtt tgtgtgccgt cttcaataat
     5881 tgctaaaatt tctatttttt cttttggcgc acggacttct tgaggacgaa ccattttcta
     5941 ttcctccctt atctcattac ttcacgacca acacactgca aggggcatgt gatacgacat
     6001 aagaagcggt cgatccaact aatgcttgtt gtatagcacc ttttcctgtc gctcccataa
     6061 cgatcaaatc aatattttct tgtccaggga tgacattggc aatcaaattt cttggatctc
     6121 cagtttcaac tattgcgtgg atctcagcaa tgccgtactc tttggcatca ttgatttttt
     6181 tcagcatggc tacttctgtt ctttgttttt catctgcata gatttttgaa aaggcatagg
     6241 cactatgggt cagctcaact ttgttgatga ttgacacgat aaataagtgg gcttcattgt
     6301 cctttgctaa gtcaagggct tcagcgaacg ctttgatcga ttgtcttgat ccgtcaactg
     6361 ctaccatgat gttctggtat cttttactca ttggaaccgc ctcctttttc ttttattgta
     6421 acatctcaag caaaaataca aaaaatatac gcaatatgta aataaaatga tgaacaaaag
     6481 caatttcttt caagttattt atacatttct tttctcatca gaacacctgt tctaatccca
     6541 ttatttctgc attatgtgaa attcctcatc atttttcaca tcgagcgaac atcattctgt
     6601 tacaaaaaac gtggatttgt cataaattgt catgcttctt ttacctatga aatattttca
     6661 cgtgttaaag tattcctcgt tgaacacaac aaataaaact ttttaacgaa taaaggagat
     6721 ttttcatgac atcacttaaa actttacttt ttggaacaac tttggctgcc ggcgctgcat
     6781 tcttcatggg aacgactgct catgcggacg aagcttatac cgttcaatca ggtgatactc
     6841 tatcaacgat ttctcaaaaa tacgttggtg ataactcctt gatcaatgcc attgcagaat
     6901 caaattcaat ttctgatatt aacctgatct actcaggaca acaattaaca atcccaacag
     6961 aaggctctgc acaagcagct gctgaacctc aagcagccgt tcaagaagca ccagtccaag
     7021 ctgaacctgt tcaagctgaa caacctgttg tacaagaaac ggttcaaacg gaaacacaag
     7081 cagcgcctgt tgctgaaaca caacctgctc ctgctgtaac agaaactgct gctactccag
     7141 caagtacaag ctctgcaaaa gaatggatcg cacaaaaaga atcaagcggt tcttacacag
     7201 caacgaacgg acgttacatc ggccgttacc aattagattc ttcttactta aatggtgact
     7261 actctgctgc aaaccaagaa agagtagcag aacaatatgt tacttcacgt tatggttctt
     7321 gggaagcggc gaaagcattc tgggaagcaa acggttggta ctaagattca ttatttgaac
     7381 gtttaaacac attcaaaata tagtaagagg tcatgcgatt tattctttcg catgacctct
     7441 ttttgagttt aagtttagtc tttattcctt aactatcgtt tgccagtatt tcttgtatca
     7501 gaatctataa atctactatt tgaatcaatc tattaggttt taaactgtcc caccttcaca
     7561 caaatttttt ttgatttatt tgaatctggt tccgttgttt aaaatttttc ctatattctt
     7621 ttagataata tgttattttc tttctcatcg ctccaggtgt accttctctt tataaaaaaa
     7681 caaattatgc ggctaagaag actcgaactt ccacgaggtc accctcacta gcgcctgaag
     7741 ctagcgcgtc tgccaattcc gccatagccg catcacatct tttagtttag gattacatgt
     7801 gttttttgtc aagaacatat ttttgttctt tttcatttct gtacgccctc ccttatgcca
     7861 tctacacaaa taattcatcc gagcaactcc atcatctatt gagggttggt gaaaaaatct
     7921 gttttatcca tatgatcgat ttcaatgtta aacgaacgac tctgggatta gtaactattt
     7981 tatttttttc ttcatttctt ttttcctctt tttctcgatt cagctatctt ctttcatttg
     8041 gatattgatt atttcttctg ttgattacta tccttctgga attctagacg ctgaagtaat
     8101 tcacgttttc tttcttctag agatcataag taacgaatga acgaacttca aataattttt
     8161 ttattgtaat tcatatttgt attgttcgat gattcttaat acgatatgac acaataaata
     8221 aaaataggca tggctattta caacaatagt gctatagtag aaaacaaatg aataaggtta
     8281 cttaaagagg tgaatacgat gaaactgatc aaactatggg aagcagattt aaagaaagct
     8341 tatcagctac aaactagctt tggaccaaat gagagtggat ttatcaacaa tgcttatggc
     8401 tatacatttg ctgaattttt agattatgta aaaaaatgcg aaaaaacgtc tttaggaatc
     8461 gatcttcctg agagttacgt tcccgcaacc gtctttattt tagaaagtga tgaacatgag
     8521 tatgtaggaa tttttaattt gcgccataaa ctgaacgaaa cattaagaaa cggcgctggc
     8581 catattggtt acggcatctc ccctaattat cgtaaaaaag gatatgccac acaaggatta
     8641 gcattagtac tacaagaggc aaaaaaaatc ggcattgatg aggcactttt gtctgtaaat
     8701 aaaaaaaatc ttggcagtct taaagcacaa caaaaaaatg gggctgcgat ttatcgtgaa
     8761 gatgagaaag aatactatac gaaaatcaaa ctgatgtaaa aaagaaggat tccaaaagga
     8821 aagggatttg aatgtccttt atcaatagaa cacatttatt taattaactt ataatatagc
     8881 agacaaaatt ttataaacaa agaaaccttt tatctctttt cttctagaaa ggataaaagg
     8941 cttttttgtt tttcctattt gcttctcata gctagtcagc caagatttct atcgtatccc
     9001 caacgcaatc cgtgcataac ggctcatctt atctgtcgtc catgctggat accagactag
     9061 tttgacttct gctttcgtta cttctggcac ttcttttaat gcgtcatgga tagattctgt
     9121 taaaatatct gctaatggac agcccatagt tgttagagtc atcttaatga ctgtttctcc
     9181 attttcagga ttgaattcta tatcataaat caagcctaaa ttgacgatgt cgattcctaa
     9241 ttcaggatca atcaccattt ccaaggcagc aagaatacgt tctttgatta gttctatttc
     9301 ttgtccagac cgttcttggt ttgcttcgct catcgtacga cactccttaa cacgattttt
     9361 atttatcata ctcttttttt agctgcttgt aaatgagaat ttagaagtaa gattctcgtt
     9421 taaatttata aaaatgttct tattagatgt aatagattaa atttattcta aaataaacct
     9481 gattcctcat tttttttgtg aacgattttt tatttctggc aatacgccag ttgttcgata
     9541 cattttttgt tcgttttcat ttgcaaaccg gccaaatact gaatgagttg gtaaaatatt
     9601 gataaatgca tcgtcgtcga tttcatgaac gatctgatca agatcataca tttcgtagcg
     9661 ggtgatgacc atcatgatca tgttcccttg tttttttgaa tagccaccga cagacggcag
     9721 taatgtcatt ccacgtacca tccgttttgc tagttcttct gttacctctt ctggcttgac
     9781 ggtaacgatc attgctgtta ctttttgatg actcgtgtgg atggcatcaa ttacttgagt
     9841 cattgcataa atggaaataa tcgtatacaa ggcactttcc cagttaaaga agaagcctgc
     9901 gatgactaca atgataccat ttagaatcag catgtagttt ccgactgttt ttccagttgt
     9961 ttgtgaaagt acaagagaga tgatatccat cccaccagtt gtaaagccca tttttaaaga
    10021 taatcctacg ccaagaccta ataaaacacc gcctactagg gcattcatca aaatattatc
    10081 agtgatctgt ccgactggaa gcagcatcgt catcaccgat acacatacaa cattcacaaa
    10141 actaaggatc gttgctcgtt ttcctacttt gagaaaccct aatacaaaca cagggatatt
    10201 caaaagaaaa ataaatgttc ctgtgtctat cttgatatga aaccattcat ataaaagcga
    10261 ggaaatgatt tgagcgatcc cattcatccc tgcagaaaag acattggctg gaatcaaaaa
    10321 ataatttaaa gcgaccgcag cggtcaatcc ggttatcaaa ataaccacta ttttctttaa
    10381 tctttcattt ttatgatacg cagtcataat tttactcaca agcacctctc ccctactttg
    10441 gtattccaaa tgtaagaata acagtttatc tattaaaata aaatacttcg aactcaaatg
    10501 agaagaattt tagcaaacag gtaagaatct gccctaatcc tatgataaga tagatgtatg
    10561 gtaaatattt atatgtatat caggagggtt cctatggcaa aagatgctag cttcgacatt
    10621 gtatcagaag tcaatacaga agaagtaaaa aatgcgattc agcaaacaac aaaagaattg
    10681 tccaatcgct ttgacttcaa ggattctaca gtagaaatca aattagagaa cagcaatcta
    10741 gttgtattga gcgatgatga tttcaaaatc gaacagatca aagatgtatt atttggtaag
    10801 ctgatcaaaa gaaatgtgcc aatcaaaaat atccattttt ccgaatcaga acacgctcta
    10861 ggtggcaaag ctcgacaaac tgccgatttg gtcaacggaa tcgatcgtga aaacgcgaag
    10921 aaaatcacta cagctatcaa aaatgaaaaa ctgaaagtaa aagcacaaat ccaagaagat
    10981 cagattagag tgacaggtaa gaaccgtgat gatctacaag ctgtgattgc tttattacgt
    11041 aaacttgact taccaatcga gttgcaattc actaactatc gttaatcaaa aaacgaggct
    11101 gacgtctgtc attcctcgtt tttttgattt actgcttctt ttgataacca aaaacgctgt
    11161 attccttctt ccaattcccc aacacttcgt acttctcttg ctgtttgcca atgttgtggg
    11221 aataaacgtc gataagaaaa actagaaaat cgttgatctg ccaatacaac gattccttga
    11281 tccgccatgc cacgaatcac tcgaccacca gcttgcaaga ctttgttcat tccaggaagc
    11341 tgataagcgt aagcaaatcc aaggtgttcc ttctcatcat aatatgattt gatcagttcc
    11401 tgctcatgat tcatctgcgg caaacctaca cctacgatca tactgccgat caatcggtcc
    11461 cctctcaaat cgattccttc tgaaaaaatc ccgccaagta cgcaaaatcc aatcaatgtt
    11521 tcagcgggat ttgtttgaaa ttttgttaaa aatcgttcgc gttcttcctc attcatgtcc
    11581 gtttcttgga ttagcacatt gctgtcggga tattgctgtc tgaatgcttc caccgcaaga
    11641 tctaaatatt gataagaagg aaagaaaacg aaataattcc cgacctttcc atcagcaaaa
    11701 gcatgaatcg tctctactaa ttttgccaga ctgtgttccc tttttcgata agtcgtctct
    11761 aggtaatttg cgatgaaaac ttgctggttt ccttctggga aaggactcgg cagtttgtaa
    11821 gccagacttt tttgtccccc cagtgtttcc tgataataag agagtggtga aaaactagca
    11881 gaaaaaagaa tactgctttt tcctttatcc aacgactgct caagaaacaa gcttggatca
    11941 atacaaaact cttttactat cagatcgcga tacgtttttt cgactgttgt ttcgtagtga
    12001 tcatcataaa attcactgac cttcaaaaaa tgaagcaaat caaaataata aggaagcaat
    12061 tgttcttgtg atgggtgctc aggaaattct gccagccatt ctttaatttt ttcgcttaat
    12121 tgataacctg cctttactaa agattcagcc ggtgcttttt gatgatgata atcccaatga
    12181 tcttcttttg ctatttctcg gatcgaatca aattctttta atagtttgtt gaaacggcga
    12241 tgaagttttt tgaattcctt tggtatagct tccttacttc gtttcgtttt ttcataagat
    12301 agttctgccg aatacatctc tcgggaacga ttcaccaaat tatgtgcttc gtcaatcaaa
    12361 aacagatagt cctcgttttt ttcatcttca aaaaaacgtc ttaaataaac agttggatca
    12421 aataaataat tgtaatcccc aatgattacg tcacaccaga tactgacatc taatgatagt
    12481 tcgaaaggac aaagtgtatg ctttcttgca taagtttcga tgacttcacg agtgatctga
    12541 ttctcatgat tcaaaagatc ccataaacct tcattaatcc gattgtaata accattggca
    12601 tatgggcact gatctggatt acaggtcgtc tcatctaaaa agcagatctt gtctttcgcc
    12661 gtcaaagtga cactttttac ttcagctcct gtatctttta atgctgtcat agcatcttca
    12721 gctacttgtc tagttattgt tttagccgtt agataaaaga tcttttctcc ctcttcttcc
    12781 cctacagctt tcaaagcggg gaataacgta gagattgttt ttcctgttcc ggtaggtgct
    12841 tctacaaaca atttctgttt cgtacggatc gttttataag cagctaccgc tagttcacgc
    12901 tgacctttcc tatactcatc atgggggaat ttcaaagcca tcaaagaggc attgcgtaca
    12961 gtgcgccagt tttcttgaaa aacaagccat tctttataat cttcagtcag ctttttaaaa
    13021 aaagtttcta actgctctct tgtctgatgt tcaacctttc tcgtgatcac ttcttctgcc
    13081 gtctggaagt aagtcaattg cagatttatt tcttgtaaat catgttcttg actatagata
    13141 taggcataca cccttgcttg atgaaagaaa agttccactt gttctggttc taaatcttca
    13201 aatcgtggtt cggaggtttt gatttcatct atataataaa taccgtctct tttgaaaata
    13261 ccgtctgcac ggccttcaac gatcaggtaa tattcatcta attgaactgt tgtttttaaa
    13321 aagacttctt tttcatagtt ttctcccgca tctttttgga gcttgcggtg gattctcgct
    13381 ccttctaatg cagtatggtt acttttttta cgattgtcga tacttccacg tcgcaaaata
    13441 aattctacta agtgccgcac agatattttt gactgtttga tcatttccac cacctctttc
    13501 tctctaagat tacctgaaaa aacctagttt agcaaaaaga gctctctttc caaacataaa
    13561 aaaacaccct gctgattagg cagggatata ggatatagaa tttggatttt tggatgaatc
    13621 taatgtaaca aggacatatt aaaatgctat gaaaacttac tttccttctc tttagaatat
    13681 ccaaatactt ttcatatagt ctgataattc agataagtaa agagaaataa gtacctgttt
    13741 ttgtttccta gcaatgaatg cgttatacta atggatgaaa gaagttttgg ctatcaagga
    13801 ggaatgcaca tgcatatcga aagattaaaa acaggaatga ttgaagaaaa ctgttatttg
    13861 gtctacaacg aagaagcact tttgatcatt gaccctggag aagatgcgga acgaatcaaa
    13921 caagaaatca aaaaaacaaa ccaacagccg gtagcgattt tgcttaccca tactcactac
    13981 gatcatatcg gtgcagtgga acctttacgc gcgtattatc aaatcccagt ttatgtcagc
    14041 ccactagagc aaaaatggct aggtgatccg attttgaatc tttctggact cggtagacat
    14101 gatgatattg ctgatgttat tgtctcgcct gcagaatatg aatttgaaat gaaaacttat
    14161 actttaggaa acatgtcttt tcgcgtcgtc cctaccccag ggcattctat aggaagcgtc
    14221 agctttattt ttgatgattt tgtcgtttct ggcgacgccc tgtttagagg aagtatcggt
    14281 agaaccgatc tgtatacagg gaatttagaa caattacttc atagtattcg gacccagctg
    14341 tttgtattac ctgatgaatt tgcagtctat ccaggacatg gcgatgcaac gacgattgaa
    14401 caggaaaaaa ggacaaatcc ttttttcaat cgttaaaacg acttaggagg agaataaaat
    14461 gacagaaact acactttata tcgtacgaca cggaaaaacg atgttcaata caattgaacg
    14521 agtacaaggc tggtgtgata cacccctcac aaagcaagga caagaaggca ttcattattt
    14581 aggtcgcggg ttaaaaaatg tcgacttttc atttgcttat tctagtgaca gtggtcgtgc
    14641 aatcgaaact gcccgtatta ttctaggaga acattctaag ggaaaagaaa ttccttattt
    14701 tattgaaaaa aggattagag aatggtgttt cgggtctctt gaaggtggat atgacatgga
    14761 aatgtggggt gtgatccctc gtgtattgaa ttttaaaaca tatgatgaaa tgtttaccac
    14821 agaagtttct tttgaacaaa ttgctaatgc tgtatacaac ttagataccg ctgggtgggc
    14881 ggaaacttat gaaacattgc gagaccgtgt atggacagga tttgaagata tcgcccatca
    14941 ctgcgaaaaa aatggtggtg gaaatgtttt agtcgtatct catggtttga cgatcgcctt
    15001 tttgcttaat ctgatcgatc ctaaacagcc tgttcgtgct gggcttttaa atggcagcgt
    15061 aacaaaggtc acttatgcaa acggtcgatt ttctatccac ggcatcaatg atacgtctta
    15121 tatcgataac gggaaaacac atagtcgcga ctcgtttcta taatggggat attttaattt
    15181 ttaccagaga atcataaaag gcaaagtctg aacgagggct ttgtcttctt ttttctcttc
    15241 gtgtacttat ctgctttatt tctcaagttt ttcttaatat taaaaaacag attgggtttc
    15301 tcaaaaaata acggtatact ataagatgta tcttaattgg gaaggaagga atttatgttt
    15361 tttgcaaata ttatcaaagc agtgattctc ggtattattg aaggaataac ggaatggctg
    15421 cccatcagta gtactggtca tttgatccta gcagatgaat tcatcaaact ggatgccagt
    15481 tcacaattta tgtcgatgtt caatgtcgtt atccaattag gggcgatctt ggctgtcgtc
    15541 gttctttatt ttcacaagtt aaacccattt tctccaagta aatcaacggt agaaaaaaaa
    15601 gatacgtgga cactttggtt taaagtgatc gttgcttgca taccagccat catcattggt
    15661 ttgcctttgg atgattggtt agaagcaaat ttccacaagt tcttaccagt tgccattgtt
    15721 ttgatcgttt acggtattgc ctttattatt gtcgaaaaac gcaacaaaaa caaaacacct
    15781 cgttggactt ctctgaatga ctttacgtac caagcagctc ttcttgttgg gttattccaa
    15841 gtcttggcac ttgttcccgg aacatcccgt tctggcgcaa cgatcctagg atctatcttg
    15901 attggtgctt cgcgttttgt cgctacagag ttttcatttt tcttagggat tcctgtcatg
    15961 tttggcgcaa gcggcttgaa gatcgtcaaa tatttagctg aagggaattc attcaaaatg
    16021 gaagaaactg ttgttcttct tgtaggaaca ttcgtatctt ttgtggtttc cgtcttagct
    16081 atcaaattct tactaagcta tttgaagaaa aacgacttta ccgcttttgg ctggtatcgt
    16141 attatccttg gtatcatttt gattggttat tggtttattg caatgtagaa aaaaccagaa
    16201 cacttctctt
//
LOCUS       DACDTO010000062        16186 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDTO010000062 DACDTO010000000
VERSION     DACDTO010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16186
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.062"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..466)
                     /locus_tag="IWK70_001999"
     CDS             complement(26..466)
                     /locus_tag="IWK70_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9614514.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            626..1132
                     /locus_tag="IWK70_002000"
     CDS             626..1132
                     /locus_tag="IWK70_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9614515.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            1300..3213
                     /locus_tag="IWK70_002001"
     CDS             1300..3213
                     /locus_tag="IWK70_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9614516.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            3218..4219
                     /locus_tag="IWK70_002002"
     CDS             3218..4219
                     /locus_tag="IWK70_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9614517.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(4421..4627)
                     /locus_tag="IWK70_002003"
     CDS             complement(4421..4627)
                     /locus_tag="IWK70_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9614518.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5273..6121)
                     /locus_tag="IWK70_002004"
     CDS             complement(5273..6121)
                     /locus_tag="IWK70_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9614519.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            6285..7187
                     /gene="gnd"
                     /locus_tag="IWK70_002005"
     CDS             6285..7187
                     /gene="gnd"
                     /locus_tag="IWK70_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9614520.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            7189..8190
                     /locus_tag="IWK70_002006"
     CDS             7189..8190
                     /locus_tag="IWK70_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9614521.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            8187..8471
                     /locus_tag="IWK70_002007"
     CDS             8187..8471
                     /locus_tag="IWK70_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9614522.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            8504..9301
                     /locus_tag="IWK70_002008"
     CDS             8504..9301
                     /locus_tag="IWK70_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9614523.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            9315..10148
                     /locus_tag="IWK70_002009"
     CDS             9315..10148
                     /locus_tag="IWK70_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9614524.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            10166..11023
                     /locus_tag="IWK70_002010"
     CDS             10166..11023
                     /locus_tag="IWK70_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9614525.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            11045..11554
                     /locus_tag="IWK70_002011"
     CDS             11045..11554
                     /locus_tag="IWK70_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9614526.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            11591..11992
                     /locus_tag="IWK70_002012"
     CDS             11591..11992
                     /locus_tag="IWK70_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614527.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            12104..12457
                     /locus_tag="IWK70_002013"
     CDS             12104..12457
                     /locus_tag="IWK70_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614528.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            12491..12934
                     /locus_tag="IWK70_002014"
     CDS             12491..12934
                     /locus_tag="IWK70_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9614529.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            12951..14036
                     /gene="uxuA"
                     /locus_tag="IWK70_002015"
     CDS             12951..14036
                     /gene="uxuA"
                     /locus_tag="IWK70_002015"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9614530.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            14029..14742
                     /locus_tag="IWK70_002016"
     CDS             14029..14742
                     /locus_tag="IWK70_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9614531.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            15494..15694
                     /locus_tag="IWK70_002017"
     CDS             15494..15694
                     /locus_tag="IWK70_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9614532.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         5616 a         2514 c         3413 g         4643 t
ORIGIN      
        1 gaaaagcaga ttgtttatgc caaaattaag acattgcaaa aagattattt tctttaaaat
       61 cgacaagatg accagaaagc aagagttcgt tgagctcttg ctttctttta gttttagaaa
      121 ggaacatcct gattttatct tcgctaaatc cagtcaaaag ttttgcagat tggtcgtaga
      181 taatcggttt tttcaataaa gaaggggatg tacgaatctc aatatatagt tcactcaaac
      241 tgagttcatc tattctgtga acaagatttt tataactaat agaatgagta gagagcattt
      301 cggtcacgcc gttgtttgtc aattttagta agtggataaa ttcatttttg gttagtggtt
      361 gacgaattaa gttctgttcg ataaacggta tatcatggac gataagccaa cgttttgcgc
      421 gtcttgaaga cgagcagcca gaacctgtat acaatttaat catcatacat tcctccaata
      481 gctaagtatt tatgaaataa taacatgaag aaatagtcga aaagtgccat ttagaggtgt
      541 aaaataagaa aatgttgtca cgtataatgg aactagctag ataagaaata ctattaaatc
      601 aagaaaaaag aaagaaggca caagtatgtc aaacacagcc gacgcaatag tagtgatcga
      661 tttacaaaaa ggggtatgtt ttgattcttc gccgatacac gaattagaaa cagtgacagc
      721 attagtgaat aaaagaatca aaacatacga aacgattggg aaaccagtta ttttcgttca
      781 acacgaagat gctcaattag tgccacaaac aaaaccatgg gagctattgt cggaattatc
      841 tattcctgag cacagttatt ttgtgagaaa aacccatgcg aattcatttt ataagactaa
      901 tttgagtgtt ttattaactg aattggactg tcattcgatc gaattatgtg gggcacaagc
      961 agagtattgt atagatacga ctgtgaagtt tgctcatgga ttaggatacc agcttatgat
     1021 gtgtagagga gcaacgacaa cttatgataa tccttttatg aaagcaaagg aaacaatcgc
     1081 tttttacgag aaaatttggg ataaacgatt tctgacattt ttggagcaat aagttgtttc
     1141 ttcgtttaaa aaaagcagaa tggatactgg tttatccatt ctgctttatt acgtaaaatg
     1201 agaaaataat cctgttctat ttttatggga taaaccattt attttttgta taaaaaatgt
     1261 ttgcgtttac atataattct cctagaaaca taaaaaggag tgaatagctt gagaacaaaa
     1321 caagtattaa agggagcgtt ggtcatctta tttgctgtaa gtacaagcgt cccgcaatgg
     1381 acagtatatg gagagagcat ttcttccgaa tcggaatctc gggaggtaga aaccacagaa
     1441 actactgatg ctggaaatga aacgaaatcg agtacttcac aaacagaaac aaatactgaa
     1501 acaacggttg agacatctgt tactagcaca gatcaagaaa cgaccgctac cacagataac
     1561 caacaaacag aagaagcaac agctacaaaa atgtcaaacg acgaatcctc ttctgctaaa
     1621 gtaagtgaca aagagaatgg gacagaaaat gatgatatcg gtaaaggtga aaatttcaat
     1681 ggtaaagaat atttgaatta cttcggtaaa cagatgacag aaatacttaa tggaccaaca
     1741 gaagacatca agtcaaaatt aatagagtta ttagtcactg agcttgctgt taatgattat
     1801 ggtgtagata aagagattat caaacatctg attgctactt atctattgta cgatatcgct
     1861 tttgcaaaaa acagtgtgtt gatgtcgatg cttggaggta cagatgatgg gacaacgcct
     1921 acagaaaaca caggcttcta tccaaataca aaacaacttc atcaaactgt actaaacaaa
     1981 caagatggca aaatgatgga tgtatatgca tattatgtcg atcaaggttc agataaaaca
     2041 gtcatgatcc atggcggatt tcgagggaat tggaataacg ggatcgttac agaagaatac
     2101 aatgattttt ataaggccgg gtataattta ttatttgttg attccagagc gactggtaat
     2161 agcggaggcg attatgtgac ctatggacaa tatgagtcag atgacgtcct ttactggata
     2221 aatcaagaag ttcgagagcg gccatctcaa aagattttac tttatggtgg ttcgatggga
     2281 gcagctacga tgatgtctgt attagcgaaa gatattcctg tcaatgtcaa aggaattatt
     2341 gaaaattgcg gatttgcatc aattgatgaa caactacgtt ttacgtacag ccagacagta
     2401 gcccccgcac taccggatgc cataaaaaat cagttagaca tcattgggga tcaagagcat
     2461 gaagatttat ttatgggcct tttgaaacaa tactattttg atcaggagat gcatttggat
     2521 acaaaagctg ctctacctac gattggaatg tcagattcat tgcctaaatt gattatccat
     2581 ggaacagcag atgatgtagt acctgtgtct aacgcgcaaa aattatatga gttatcagga
     2641 gggtataaag atcttttatt agtagaagga gcagggcatg gaaaggcaca ggaagttgac
     2701 catgctgcct acacgaaaca tgtaacggac tttttgaaag tagtgttcca tgatcagata
     2761 aacgtaaagt atgtggatga aaacaataaa agtctactca aagaccagga cgaaatccag
     2821 ctttacggag cttatggaga aaattatatg acagagcaaa agacattcga aggatacgaa
     2881 ttagcaaatg ttgaaggacc gactgaaggg atttttaatg agacgatacc aactattatt
     2941 ttctattaca aaaaaatccc agtagctcca ccaaaaaagc aagatccgac agagaatgca
     3001 gacaataagg gaaagaatga acctcgagag gatcaatcaa gtgatgatac gcaaccgatt
     3061 cagatgacag tgaaagtaca aaaaaagaca gatgcaaaaa aaagaatgtt gccgaaaact
     3121 ggagaaaaga aacattcgct tctcatttat tcaggtggat ttttatctgt tagcacgatt
     3181 atcacatgga tttggcgaag aaagagaggg taaatgaatg aaatggttca agcgaatagt
     3241 gattgcagta ccagttatca tcgtagttat tttagtcact gtatttttga ttaatcaaat
     3301 cacaccaaag ccgattagct tgcttgttag aaaacaattt gacacaagtg aaaaagaaca
     3361 agtatatgcc cgaccagatg atttccagca aaaaatcgaa caaatcgata taaaaaaaga
     3421 aatatcctat ccttcaaagt acaaaaataa taatatggat atctacacac cgaaagtgga
     3481 aaagaaaaaa ttaccgattc ttttctggat gcatggaggc gcatatgtag gaggggataa
     3541 aaatgattgt cgtgattatt tggaatatct atgttcagac acgcagcaaa tcatcgtaaa
     3601 tatagattat gaacgatcac cggaagcaaa acatcctaca ccagttatcc aattgaacga
     3661 agccattcaa gcagctaaaa aagagataaa agatcaagca gattggtcga atatttcgat
     3721 tggtggaaat tcggcaggtg cacaaattag tggagaatat ttgttagcac ttcaaaatga
     3781 agaaattaaa aaagctgatc atcttgatcc gactttggag aacaagcaga ttacgaagtt
     3841 tatttctttg tctggtctgt tggatccttc tgcacttact caagtaagtg ataaaatctc
     3901 tagctttctt tatgaaaaat gtggatgggc ttactttgat aataaggact ttgaacaatc
     3961 agagaaggtc aaaagtttag ccttggtcag acatgcggat cgttggacgc aaaacacatt
     4021 cttaacggat ggaaatacaa atacgtttac gaaacaaatg gaagaagtaa cttctgtatt
     4081 tgagaaagcg ggggtgaaag tcacaaaagt agattatgcg gagaaaaatg ctgttttgaa
     4141 ccatgaatat cagtttgatt tcagcaataa acaagcgcaa gaaacatatc aaaagttagt
     4201 aatgtttttc aaggagtaaa gtcttatgat gcaaaagagg ttgtaatctg tcacttagct
     4261 ccaagtcaca aggagcaatt tcaataaaca agcttttcat ggtttatgaa tattgtaact
     4321 tgtgactgag gagttagaga tggcaagggg caccttttat tcgaaatttg ggaatatcac
     4381 acgattattg tcacgatctc ttttacgtaa attttataag tcagatttgt tgttttgatc
     4441 gtttaaaaaa agcaacaaat ccgaagcctg cggaggcaag aagggacaaa aagccgagtg
     4501 gtaagcaaaa gaatgctggt tccactaata ttccgttgtt attgatataa ggctgggaac
     4561 aaagatgaat agcaaataaa atcaatgcac tagcgaaaaa aagcaaagaa atatggatag
     4621 ctttcataag cagcacctcc ttttttataa ttgtatgata aatagattaa gaatctataa
     4681 atgtgacaag tttgttagga tgtgaataaa atgatcatct agcgaacttg aaacaaagat
     4741 ggctaatgat aagcaattaa tgattcagca atcagatagg tacaaagttt accatcatac
     4801 gtctagacca ataatagaaa atatgggaat ataatagaaa agatttagga tacccccaaa
     4861 agaatttccc caaaaattcg atgtatccta aactttttta ttttacataa caatcatatc
     4921 aactgtgaaa taaaattaca aatgataacc taaaatgagg ccaaaagcca gtgttatcaa
     4981 ggaaatactt aattcaccaa aatttttttt aattttttag aaaataagaa acaaaaataa
     5041 taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat cgcatctaac
     5101 aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat acgttctaac
     5161 ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac taccaaatat
     5221 ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg tgctattctt
     5281 tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct ttttcgttgt
     5341 aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata cgggaagcta
     5401 aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca tcgccaagtt
     5461 tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca gtttttctta
     5521 cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc aaaacagcac
     5581 agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc gtgctgtgag
     5641 aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca atctctgaac
     5701 cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc gcttttttta
     5761 gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta gactcgaaca
     5821 ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg atagaaatag
     5881 cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat ccattataac
     5941 caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca cttgctaaat
     6001 tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg gcgatagcct
     6061 gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg gagacgctca
     6121 tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt aatgttaaag
     6181 atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct atttacaaaa
     6241 aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa attggtgtaa
     6301 ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac catcacaaag
     6361 tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat gacattcctg
     6421 cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc atctggttaa
     6481 tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca aaactcggac
     6541 caggagacat tattattgaa ggcggaaact cttattataa agattcaatc cgtcgagcag
     6601 aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga ggaatcgaag
     6661 gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt ctatccgtgg
     6721 aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt gcatccggaa
     6781 gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg caagcaatcg
     6841 gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa caagtcgcac
     6901 gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg gaagatcagt
     6961 taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca ggtgaagcaa
     7021 aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt gcaactagtt
     7081 tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta gtttcagcac
     7141 taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat gccagattat
     7201 catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca cttggaaaga
     7261 ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc aggaaaagaa
     7321 ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc attttttcga
     7381 cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg tattggttac
     7441 aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat catacctgct
     7501 ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc catgcttcga
     7561 cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg ggcggagttc
     7621 gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa taccggaagc
     7681 ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta tgatccttac
     7741 aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca agaattactg
     7801 gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta tcacatgatt
     7861 tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag tgcaagaggc
     7921 gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat cgcaggatat
     7981 gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt agcgtttgaa
     8041 caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga agcaatgggt
     8101 gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga acgttctgtc
     8161 cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt ttaaaagtga
     8221 aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt tctgatctct
     8281 tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca tgggaaaagg
     8341 cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat catcgttatt
     8401 ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa aaaatgaaat
     8461 cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt atagtgccaa
     8521 tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg ctccatggac
     8581 acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag aacactttta
     8641 cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc ctgttccata
     8701 tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc tcatcttaga
     8761 acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg cgaagatctg
     8821 caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac aaagaaaact
     8881 gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt tagttggagg
     8941 attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg ctatcagtaa
     9001 atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg ggattttcta
     9061 tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt tgtatcggga
     9121 attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata aacagataat
     9181 agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc ctatagaggt
     9241 aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca gaaagatctg
     9301 aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt gctttatggg
     9361 tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta cgtttttctc
     9421 ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa gcgatgattg
     9481 ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga tcaatgccag
     9541 ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg gatctgtcgc
     9601 cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac ttgtatcagt
     9661 ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta gaagatttga
     9721 atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca tttttattat
     9781 tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct gatgtaatca
     9841 aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga gggttagcgg
     9901 caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg gtctttttct
     9961 tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg gttacttatg
    10021 ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg aaacaagtac
    10081 agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac tatgatgacg
    10141 gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa caggtgctcc
    10201 tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt ttgcaaacga
    10261 aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa tgatgccaat
    10321 cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac ggcagttact
    10381 gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa tgtcttctat
    10441 ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc aggatgactt
    10501 gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag atgcaatcga
    10561 ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac aacagggaag
    10621 tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt tactaggggt
    10681 atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa agcaaaacac
    10741 tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga tgggggtgct
    10801 tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg gacgtgagtt
    10861 tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag ctgtcgtaat
    10921 gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat tattatggct
    10981 gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag ggaacgtgaa
    11041 taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat
    11101 ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg aagttgtagc
    11161 aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag gacaaaaaac
    11221 aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat ttaaagaata
    11281 caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa aacacggaat
    11341 ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata cagatctttt
    11401 agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag aaatgaatga
    11461 acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta aaacaggatt
    11521 aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa aagataaaga
    11581 gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt atcgctatat
    11641 ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat tagtatcaga
    11701 tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag cctaccgatc
    11761 aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta cttattacga
    11821 tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc aaaggaaaaa
    11881 aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt ggtggggaat
    11941 gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat aaacaggtat
    12001 cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc gcggatggcc
    12061 tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag aaatgatccg
    12121 tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa
    12181 gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt atctagactc
    12241 tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata aagaacagtg
    12301 gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa
    12361 aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa catttaattg
    12421 gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa aacaaggagt
    12481 gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg aaggactgaa
    12541 agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt gcttagagtc
    12601 tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta tgatcaacac
    12661 agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca atcaagcgct
    12721 cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt
    12781 gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac cgatagaaga
    12841 agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc atgcttcagc
    12901 ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag atgaagtggg
    12961 gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt cgccaaatac
    13021 cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat gtttgggaga
    13081 tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa ctgcttggaa
    13141 ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga gattactata
    13201 ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga ttgatttgtt
    13261 atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa aacaacgatg
    13321 gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt
    13381 atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa gaagaacggt
    13441 tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa gaattatttg
    13501 aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa
    13561 tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga gtgacgaaaa
    13621 atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat ggactaacgc
    13681 tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc atccatgagg
    13741 taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga gaaagaaagt
    13801 tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat attatgaaag
    13861 cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga accgtgtggg
    13921 gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc acttacatgc
    13981 aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat gaataaaatc
    14041 aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt agcttatcgt
    14101 gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt ctattattta
    14161 ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta cggagaagat
    14221 gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa
    14281 gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc aaaagaaaga
    14341 ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa
    14401 atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt aaaaaaagga
    14461 aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa
    14521 gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc gggcattttg
    14581 tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa gcataagttg
    14641 aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta tgacctgcat
    14701 caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg actattttct
    14761 agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga agtaattttt
    14821 tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca aaacgcatct
    14881 actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca tatgagggtt
    14941 tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt aacacctgtc
    15001 gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc tgtatccaag
    15061 gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct ttctttaaat
    15121 acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt ataccaccaa
    15181 ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa aagcaaataa
    15241 acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga ttcgaagaca
    15301 atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag aaaaagcatg
    15361 atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct acatagataa
    15421 aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga tacgtatcag
    15481 gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca aaggttttgg
    15541 ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta tccaaggtga
    15601 cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag atggtcaacg
    15661 tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta accgtctttt
    15721 cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg gtcgttgaat gggctgaata
    15781 gtagtgtata aaacaatttt gtggagcgtg aagcttttat ggtagaggaa aatacgaata
    15841 aagtgatgtt gataaaaaga ttcctatctt gaatatgaat cgagatgtct tgaaaatgaa
    15901 tgagtttgac caatcattat cctatcgaaa tttacagttt cgttcgattg gtctgtcaat
    15961 taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc ggcacatcta aatttttaag
    16021 taacggacag ttcaaatatc aagcattgaa agaggctatc ctactgtttg aaggaatacg
    16081 aatatataaa aacgacaaag aggaaaaagt agttcattaa atcccttcaa gtgatgaaag
    16141 aatgagatct aataaaacct gatctttcat ctgttttttt aataac
//
LOCUS       DACDTO010000063        16135 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDTO010000063 DACDTO010000000
VERSION     DACDTO010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16135
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.063"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1233
                     /locus_tag="IWK70_002018"
     CDS             <1..1233
                     /locus_tag="IWK70_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9614533.1"
                     /translation="HLFFDEEWLIEKEYKGVQAQFIKGKLDDSPSYCEHCRSVRIIRN
                     GSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILM
                     ELYENQSRKLIAKKYFVSDGTVTRILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGS
                     MSFICVDGTTHQLFTILEDRRLYKLTQYFLHFPRKARLKVKYLVMDMNASYCQLLKTV
                     FPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDST
                     TWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLRYYFVKRGTEGFQET
                     LKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNNKIKLIKRIAFGYRN
                     FYNFRARIYLQQGLIFEN"
     gene            complement(1616..2344)
                     /locus_tag="IWK70_002019"
     CDS             complement(1616..2344)
                     /locus_tag="IWK70_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614534.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(2322..3896)
                     /locus_tag="IWK70_002020"
     CDS             complement(2322..3896)
                     /locus_tag="IWK70_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9614535.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            4125..5063
                     /locus_tag="IWK70_002021"
     CDS             4125..5063
                     /locus_tag="IWK70_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9614536.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            5075..5986
                     /locus_tag="IWK70_002022"
     CDS             5075..5986
                     /locus_tag="IWK70_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9614537.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            6010..7482
                     /locus_tag="IWK70_002023"
     CDS             6010..7482
                     /locus_tag="IWK70_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9614538.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            7533..8144
                     /locus_tag="IWK70_002024"
     CDS             7533..8144
                     /locus_tag="IWK70_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9614539.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            8174..10087
                     /locus_tag="IWK70_002025"
     CDS             8174..10087
                     /locus_tag="IWK70_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP9614540.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            10215..13196
                     /locus_tag="IWK70_002026"
     CDS             10215..13196
                     /locus_tag="IWK70_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP9614541.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            13193..14854
                     /locus_tag="IWK70_002027"
     CDS             13193..14854
                     /locus_tag="IWK70_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP9614542.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            14894..15826
                     /locus_tag="IWK70_002028"
     CDS             14894..15826
                     /locus_tag="IWK70_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9614543.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         5229 a         2610 c         3232 g         5064 t
ORIGIN      
        1 catttatttt ttgatgaaga atggctgatt gaaaaagaat ataaaggcgt tcaagcgcag
       61 tttattaaag gaaaattgga tgactctcct tcctattgtg aacactgtcg atcggttcgt
      121 attatccgca acggttccta tacgacacgc actcagatat tgaaagtcaa agaaaagtta
      181 actattttag aattaaaacg tacccgtttt ctctgccatg attgtggaag gaccttttct
      241 gcgaaaacgg acttagttga tgaacatcat caactaacaa aggaactgaa gcaggcaatt
      301 ctcatggaac tgtacgaaaa tcaatcacgt aaactaatcg ctaagaagta cttcgtctcg
      361 gatgggacgg tgacacggat cttgcgtgaa gcaacgaagc attaccagtc acgtatgaac
      421 ttccttccga cggtgttgtg tatggatgag ttcaaatcaa tgaagtctgt ttctggttcc
      481 atgagtttta tttgtgttga tgggacaacc catcagctat ttacgatcct tgaagatcgc
      541 agactttaca aactaactca atacttcctg cactttccta gaaaagcccg tttaaaggtc
      601 aagtacctag tgatggacat gaatgccagc tattgtcagt tgcttaaaac agtctttcca
      661 aatgcggaga tcgtaactga ccgctttcat attgtccaac acatcaatcg cagcttcaat
      721 caattacgtg tccagatcat gaatcgtttt cgaacctcac actctgaaaa tcagaagaag
      781 taccgtcggc tcaagcgata ttggaaactc ttactcaaag attccacgac atgggagcca
      841 ctcaaacggc attatcatcg actgtttaaa cgaccaatta gtcaaacaga aatcgttgat
      901 gagctgttaa gctacaacga agaattgcga actgcgtatc atttttgtca attattacgc
      961 tattatttcg ttaaacgagg tacggaaggc tttcaagaaa cccttaaaac aatttcatct
     1021 acgttacctc agtcgtttaa aaagaaattc acgatcttcc attgctacca atcgggtatt
     1081 tcaaatgcct ttaaggtgtc ccactcaaac ggcgtcactg aggggctgaa taacaagatc
     1141 aagctgatta aacgaattgc gtttggttat cgcaactttt ataactttcg ggcacggatc
     1201 tatttacaac aaggtctgat ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc
     1261 gcatatcgga ctaagtttta ttcaaatttg tcttcaccaa cacgatttga cgaagagcct
     1321 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
     1381 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
     1441 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
     1501 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
     1561 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
     1621 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
     1681 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
     1741 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
     1801 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
     1861 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
     1921 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
     1981 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
     2041 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
     2101 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
     2161 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
     2221 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
     2281 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     2341 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     2401 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     2461 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     2521 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     2581 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     2641 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     2701 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     2761 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     2821 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     2881 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     2941 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     3001 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     3061 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     3121 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     3181 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     3241 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     3301 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     3361 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     3421 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     3481 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     3541 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     3601 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     3661 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     3721 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     3781 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     3841 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     3901 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     3961 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     4021 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     4081 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     4141 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     4201 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     4261 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     4321 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     4381 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     4441 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     4501 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     4561 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     4621 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     4681 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     4741 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     4801 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     4861 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     4921 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     4981 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     5041 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     5101 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     5161 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     5221 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     5281 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     5341 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     5401 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     5461 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     5521 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     5581 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     5641 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     5701 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     5761 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     5821 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     5881 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     5941 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     6001 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     6061 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     6121 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     6181 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     6241 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     6301 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     6361 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     6421 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     6481 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     6541 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     6601 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     6661 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     6721 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     6781 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     6841 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     6901 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     6961 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     7021 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     7081 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     7141 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     7201 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     7261 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     7321 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     7381 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     7441 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     7501 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     7561 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     7621 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     7681 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     7741 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     7801 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     7861 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     7921 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     7981 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     8041 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     8101 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     8161 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     8221 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     8281 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     8341 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     8401 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     8461 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     8521 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     8581 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     8641 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     8701 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     8761 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     8821 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     8881 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     8941 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     9001 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     9061 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     9121 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     9181 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     9241 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     9301 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     9361 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     9421 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     9481 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     9541 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     9601 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     9661 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     9721 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     9781 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     9841 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     9901 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     9961 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
    10021 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
    10081 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
    10141 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
    10201 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
    10261 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
    10321 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
    10381 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
    10441 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
    10501 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
    10561 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
    10621 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
    10681 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
    10741 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
    10801 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
    10861 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
    10921 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
    10981 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
    11041 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
    11101 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
    11161 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
    11221 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
    11281 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    11341 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    11401 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    11461 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    11521 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    11581 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    11641 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    11701 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    11761 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    11821 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    11881 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    11941 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    12001 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    12061 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    12121 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    12181 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    12241 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    12301 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    12361 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    12421 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    12481 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    12541 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    12601 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    12661 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    12721 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    12781 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    12841 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    12901 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    12961 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    13021 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    13081 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    13141 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    13201 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    13261 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    13321 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    13381 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    13441 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    13501 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    13561 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    13621 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    13681 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    13741 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    13801 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    13861 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    13921 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    13981 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    14041 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    14101 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    14161 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    14221 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    14281 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    14341 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    14401 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    14461 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    14521 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    14581 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    14641 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    14701 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    14761 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    14821 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    14881 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    14941 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    15001 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    15061 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    15121 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    15181 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    15241 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    15301 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    15361 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    15421 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    15481 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    15541 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    15601 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    15661 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    15721 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    15781 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    15841 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    15901 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    15961 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    16021 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    16081 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DACDTO010000064        15948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDTO010000064 DACDTO010000000
VERSION     DACDTO010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.064"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(387..809)
                     /locus_tag="IWK70_002029"
     CDS             complement(387..809)
                     /locus_tag="IWK70_002029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9614544.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="IWK70_002030"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="IWK70_002030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9614545.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="IWK70_002031"
     CDS             complement(2252..3154)
                     /locus_tag="IWK70_002031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9614546.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /locus_tag="IWK70_002032"
     CDS             complement(3168..4724)
                     /locus_tag="IWK70_002032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9614547.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="IWK70_002033"
     CDS             complement(4747..5289)
                     /locus_tag="IWK70_002033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9614548.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="IWK70_002034"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="IWK70_002034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9614549.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="IWK70_002035"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="IWK70_002035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9614550.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="IWK70_002036"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="IWK70_002036"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9614551.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="IWK70_002037"
                     /pseudo
     CDS             complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="IWK70_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10026..10772)
                     /locus_tag="IWK70_002038"
     CDS             complement(10026..10772)
                     /locus_tag="IWK70_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9614552.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="IWK70_002039"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="IWK70_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9614553.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="IWK70_002040"
     CDS             complement(11238..11516)
                     /locus_tag="IWK70_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9614554.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="IWK70_002041"
     CDS             complement(11629..12597)
                     /locus_tag="IWK70_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9614555.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="IWK70_002042"
     CDS             12880..13854
                     /locus_tag="IWK70_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9614556.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="IWK70_002043"
     CDS             13859..14434
                     /locus_tag="IWK70_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9614557.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="IWK70_002044"
     CDS             complement(14501..15640)
                     /locus_tag="IWK70_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9614558.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4356 a         3443 c         2856 g         5293 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgat
//
LOCUS       DACDTO010000065        15926 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDTO010000065 DACDTO010000000
VERSION     DACDTO010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.065"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            226..894
                     /locus_tag="IWK70_002045"
     CDS             226..894
                     /locus_tag="IWK70_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9614559.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="IWK70_002046"
     CDS             complement(1094..1480)
                     /locus_tag="IWK70_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614560.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="IWK70_002047"
     CDS             complement(1452..2834)
                     /locus_tag="IWK70_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9614561.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="IWK70_002048"
     CDS             complement(2990..4177)
                     /locus_tag="IWK70_002048"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9614562.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="IWK70_002049"
     CDS             complement(4184..5215)
                     /locus_tag="IWK70_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9614563.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="IWK70_002050"
     CDS             complement(5231..6685)
                     /locus_tag="IWK70_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9614564.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="IWK70_002051"
     CDS             6860..7765
                     /locus_tag="IWK70_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9614565.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="IWK70_002052"
     CDS             7856..8533
                     /locus_tag="IWK70_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9614566.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8814..9710
                     /locus_tag="IWK70_002053"
     CDS             8814..9710
                     /locus_tag="IWK70_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9614567.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="IWK70_002054"
     CDS             complement(9765..11135)
                     /locus_tag="IWK70_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9614568.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="IWK70_002055"
     CDS             11373..12119
                     /locus_tag="IWK70_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9614569.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="IWK70_002056"
     CDS             12116..12955
                     /locus_tag="IWK70_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9614570.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="IWK70_002057"
     CDS             12956..13906
                     /locus_tag="IWK70_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9614571.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="IWK70_002058"
     CDS             14047..14799
                     /locus_tag="IWK70_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614572.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..>15926
                     /gene="queG"
                     /locus_tag="IWK70_002059"
     CDS             14818..>15926
                     /gene="queG"
                     /locus_tag="IWK70_002059"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9614573.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVIN"
BASE COUNT         5160 a         2995 c         3210 g         4561 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacga
//
LOCUS       DACDTO010000066        15664 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDTO010000066 DACDTO010000000
VERSION     DACDTO010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.066"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..288)
                     /locus_tag="IWK70_002060"
     CDS             complement(<1..288)
                     /locus_tag="IWK70_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9614574.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="IWK70_002061"
     CDS             430..1548
                     /locus_tag="IWK70_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9614575.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="IWK70_002062"
     CDS             1676..3052
                     /locus_tag="IWK70_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9614576.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="IWK70_002063"
     CDS             3453..5033
                     /locus_tag="IWK70_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9614577.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="IWK70_002064"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="IWK70_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="IWK70_002065"
     CDS             complement(5659..5979)
                     /locus_tag="IWK70_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9614578.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="IWK70_002066"
     CDS             complement(6127..8361)
                     /locus_tag="IWK70_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9614579.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="IWK70_002067"
     CDS             8840..9106
                     /locus_tag="IWK70_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9614580.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="IWK70_002068"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="IWK70_002068"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9614581.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            10964..12043
                     /locus_tag="IWK70_002069"
     CDS             10964..12043
                     /locus_tag="IWK70_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9614582.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            12060..13283
                     /locus_tag="IWK70_002070"
     CDS             12060..13283
                     /locus_tag="IWK70_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9614583.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            13372..14259
                     /locus_tag="IWK70_002071"
     CDS             13372..14259
                     /locus_tag="IWK70_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9614584.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            14345..14575
                     /locus_tag="IWK70_002072"
     CDS             14345..14575
                     /locus_tag="IWK70_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9614585.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(14632..15597)
                     /locus_tag="IWK70_002073"
     CDS             complement(14632..15597)
                     /locus_tag="IWK70_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9614586.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         5077 a         2700 c         3181 g         4706 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaaa tagggcttaa gtttgagtga cagtttgtag gctatgttat actatataag
    10981 tgaaggggga cgtaaggtga aggtattatt atattttgaa agtgaaaaga tgcttgcaaa
    11041 atctggaatc gggcgtgcgc ttgatcatca aaaacgtgct ctgacggaag tcggtatctc
    11101 ttatacacta gatgaaaaag aagattatga catcctgcac atcaatacat acggaatcaa
    11161 tagtcataat atggtcaata aagccagaag agaaggaaaa aaagttgtct atcatgcgca
    11221 ttctacagag gaagatttcc gaaattcctt tatcggatcg aaccagcttt ctcctatcgt
    11281 aaaaaaatat ctggtaggat tgtaccaaaa agcggactat ctgatcacgc caacacctta
    11341 ttcaaaacag ttgttggaaa gctatggtat ccgtgtgccg attcaagcta tttccaatgg
    11401 gatcgactta gagaaatatc accctgatcc aatgaaagaa caaaagttca gagaatattt
    11461 caaactgtct ccggatcaaa aagtaatcat ttgtgtcggt ttgtttttcg aacgtaaagg
    11521 aatcattgat ttcgtagaaa ttgctaagaa gatgcccgaa tacacattta tttggtttgg
    11581 tcatgttccc atgtactcga ttcctcggaa catccggaaa attgtaaaag aagatcatcc
    11641 tgaaaatgtc ctgtttcctg gttacattcg tggagagatc atagaaggtg cgtattcagg
    11701 tgcagatcta ttctttttcc cttcttatga agagacagaa gggatcgttg ttttagaagc
    11761 tttagcaagc aaacaaaacg tacttgttcg agatattcca gtgtataatg gctggttaga
    11821 agatagcaag aactgctata tggggaagac aaacgaagaa tttatccgac tgatccaaca
    11881 gattaccaat caagaactac cgagtacgac acaagccggt tatcaaacag caaaagagcg
    11941 aagtatcaat aaaatcggtg aagaacttaa aaatgtatac gaaagtgtac tgaatgaaaa
    12001 agtctctagc aaattaaaaa aagtaaatca ggtcaaggac tagtttggag gcgaaaaaca
    12061 tgaaaatcgg cttttttacc gatacctact ttccgcaagt cagcggagtt gctacatcga
    12121 tcaaaacatt aaaacaagaa ttagaaagac atgggcatga agtctatatt tttacaacga
    12181 ccgatcctaa tgcggataag ttggaaaaag atgtgattcg aatgccaagt gtaccattta
    12241 tttctttcaa ggatcgaaga gttgttgtta gaggcatgtg gtatgcttat ttggttgcca
    12301 aagaactaga actagatctt attcataccc atacagaatt cggagcaggg atacttggga
    12361 agatggtagc taagaagttg aaaatacctc tgatccatac ttaccatacg atgtatgaag
    12421 actaccttca ttatatcggt aaaggaaaag tgatcaggca atcccatgta aaatacttgt
    12481 ctagattatt tgcaaatcat acgacaggtg tagtttgtcc aagtgaacgt gttattgata
    12541 cattacgcag ctatggtgta atagcaccgc ttcgcgtgat tcctactggt atcgacattg
    12601 aaaaattcaa acgttctgat atcactcagc aggatatcag caaattaagg gagtcactag
    12661 gtcttcagga aaaccatttg atgattcttt ctttaagcag gatttcttat gagaaaaata
    12721 tccaagcctt gattaatggg tttcctcagg tcatctccgc ttatcctaac gccagattag
    12781 ttatcgtagg aaaaggacct tatgtagaag agttagagga attgatctca gaactgcagt
    12841 tagaagaatt tgtgcagttc acaggagaag tggacaacaa tgatgtagca ctttattata
    12901 aagcggcaga ttattttgtc agtgcttcta cttcagaaac acaagggctt acttatacag
    12961 aagcgatggc agccggaaca ccttgtgtcg tggaaggaaa tgcttatttg aaccgattgt
    13021 tcgatcatcc ttcattaggg aaaacttttg aaaccgacga agactttgca ccagcactta
    13081 ttgattatat ttccagtgaa gttcctaaag atccaaaagt tttagaagct aaactttatg
    13141 atatttcatc tacacatttc ggcgaagcta tgatcgaatt ttatcaagac atggttgttt
    13201 atcatgaaga agagatgcga agaaaagaag aagaagactc gattgaacga atcaaaataa
    13261 aattaaattc atttaaaaga taaaattcaa acatttttta tgaaaaaatc cttgtttcta
    13321 gcgtatacta gcatagaagc aaggattttt gatttttagg aggtcgaaaa aatgaaactt
    13381 ggttttatcg gaacaggcgt catgggaagt gctgtcgcac gccatctact agaagcagga
    13441 cacgaagtag ctgtctataa tcggacaaaa gcaaaagctg atcctttagt aacagaagga
    13501 gctatctggg cagatacacc aaaagcggta gcagaacaaa gtaatattct ctttacaatg
    13561 gtaggttacc ccaaagatgt agaagaaatc tactatggac agtcggggat tttttctgca
    13621 gatatctcag gacatatttt agttgatttg actacaagta caccaagcct ggcagaaaaa
    13681 attgcaaaaa cagcaaaaga aaaaggcgca gatgcgttag atgcaccggt atccggtggt
    13741 gacttaggag caaagaatgg tacactgacg atcatggtcg gtggagaaga agccgtttat
    13801 gatcaagtac tgcctttatt caaagaattt ggtacgacat ttacattgca tggttctgct
    13861 gggaaaggtc agcatacaaa aatggctaat cagatcatga ttgcaggtac gatgacagga
    13921 atgacggaaa tgcttgtcta tgcacaaaaa aacggacttg atttaaagaa agtgattgag
    13981 acgctttcag gtggaagtgc agctaactgg tctttaagca attattctcc tcgtatttta
    14041 aaagaagatt acacacctgg tttttttgtc aaacatttta tcaaagattt aaagattgca
    14101 ttagaagaag cagaaaaaat ggatcttgta ttgccagcaa ctacccaagc attgaaacta
    14161 tatgaagagt tggcagataa aggttttgag aatgacggaa cccaagcttt gatcaaactt
    14221 tggtggcctg aaggaaaaat accagaaaaa aaatcataaa aaacgagttt ttttagagaa
    14281 aatcaatgca agtaggctat gcttttgata caatataaca gaaataagaa aaggaggacc
    14341 tccgatgaca cattcacaat tagtcgcaat cataaaacga ctggaggcta tggtcgagtc
    14401 agcagataat gaactgcaag ttcgccgttt tgaaaaagaa ggcgtagaga agtgtacagt
    14461 aacttatgat aaagcgaccg atacatttga attaactgaa acagatactc atcagcaata
    14521 cgaatttgat aatatcgata tcgtagcgat ggaaatctat gacttaatcc aataaatttt
    14581 aggataattg acatttgaag ttgaagatgc ctaaaagaag ccgagaagca actaatttag
    14641 ttgcttttca gcttcttttt tgtttaacag ttcaatttca aactgttctt tttctaattt
    14701 tttgcggtaa ctgatcaaga tttgtacagg atcgatttct tcttttgaca attgacgttt
    14761 caaagataaa tgagcttctt tcttttcata atagaagtca ggagaaacgt caccagaggt
    14821 aatcattgtt tctgcaagtt gaccataaca aacgaggtag tgctttttct gactctcatc
    14881 tgctgctaat ggcatgctga aggtgagcga tggtgttctt aaaaactgct tttgttcctg
    14941 gatccgtcta ataaaataag taactaattc gatatctttt tgaagcatac cggctttttc
    15001 gaaattcaac tgatagatga aatgttgttg tctttttttc aagtaagtca gtagcttttt
    15061 atttcggcct tgaaacagcc cttggagttc cttttttttc tgttcaaaag tcatttcctg
    15121 taggtcagga agctggactc gcaaagctaa caaactaatt tcagacaacc aaggcatttg
    15181 ataagtttct tccattatac gcaaaataga cggcattttt ttatagagac gaaaaggccc
    15241 gtatgttcga gccgtaggag tatctgttaa tacaaatccc ttattggtaa tatgtacata
    15301 attataatta ctgaaataat tcatttgtcg gttataaaaa ggacggtaat gttggatcaa
    15361 ttgacactcg agcaataaag catctaattc cgtgtctact acgacgaaat caaaatctgt
    15421 tatatgatga atcatacgta atactttttt tgaatgctgt ttattctgat gaaaatagct
    15481 gcttactcta tttttgagcc gttttgcttt accaacatag atgaccttac catctcggtc
    15541 tttcataaga tagacacccg gcagcatggg gagagttttc attttttctt ttaacatagg
    15601 atctcctttt acgctttgaa taaaaagact ataacaaatt ttgaaataat ggacaagttt
    15661 tagg
//
LOCUS       DACDTO010000067        15556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDTO010000067 DACDTO010000000
VERSION     DACDTO010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.067"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1044)
                     /locus_tag="IWK70_002074"
     CDS             complement(<1..1044)
                     /locus_tag="IWK70_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9614587.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELIN"
     gene            complement(1202..2098)
                     /locus_tag="IWK70_002075"
     CDS             complement(1202..2098)
                     /locus_tag="IWK70_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9614588.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(2091..2771)
                     /locus_tag="IWK70_002076"
     CDS             complement(2091..2771)
                     /locus_tag="IWK70_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614589.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(2772..3554)
                     /locus_tag="IWK70_002077"
     CDS             complement(2772..3554)
                     /locus_tag="IWK70_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9614590.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(3547..4464)
                     /locus_tag="IWK70_002078"
     CDS             complement(3547..4464)
                     /locus_tag="IWK70_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9614591.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(4628..4951)
                     /locus_tag="IWK70_002079"
     CDS             complement(4628..4951)
                     /locus_tag="IWK70_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9614592.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            5739..8966
                     /gene="fss3"
                     /locus_tag="IWK70_002080"
     CDS             5739..8966
                     /gene="fss3"
                     /locus_tag="IWK70_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9614593.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            9079..9393
                     /locus_tag="IWK70_002081"
     CDS             9079..9393
                     /locus_tag="IWK70_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9614594.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            9406..9780
                     /locus_tag="IWK70_002082"
     CDS             9406..9780
                     /locus_tag="IWK70_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9614595.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            9790..10125
                     /locus_tag="IWK70_002083"
     CDS             9790..10125
                     /locus_tag="IWK70_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9614596.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            10208..11548
                     /locus_tag="IWK70_002084"
     CDS             10208..11548
                     /locus_tag="IWK70_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9614597.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            11626..12300
                     /locus_tag="IWK70_002085"
     CDS             11626..12300
                     /locus_tag="IWK70_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9614598.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            12533..12967
                     /gene="dcm"
                     /locus_tag="IWK70_002086"
     CDS             12533..12967
                     /gene="dcm"
                     /locus_tag="IWK70_002086"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9614599.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            12968..13675
                     /locus_tag="IWK70_002087"
     CDS             12968..13675
                     /locus_tag="IWK70_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9614600.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            13665..13955
                     /locus_tag="IWK70_002088"
     CDS             13665..13955
                     /locus_tag="IWK70_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614601.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            14214..15398
                     /locus_tag="IWK70_002089"
     CDS             14214..15398
                     /locus_tag="IWK70_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9614602.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            15413..15532
                     /locus_tag="IWK70_002090"
     CDS             15413..15532
                     /locus_tag="IWK70_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9614603.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         4951 a         2831 c         3177 g         4597 t
ORIGIN      
        1 attgatcaat tcttcatcag gataaaattc atttaattta attcttacta actcttgtag
       61 ctcaacaccc atatctaaat aggaaagctt cgtttgatct aagtcgtgat tcatagcatt
      121 tttttcaatc gaagtaatgt gaatgtcaac ataagcttgt tcatctattg gcacaataat
      181 tgaaaatgct ttttcaatca ttttgattaa aataagtgta ttctttgaga tttcttcttc
      241 agaattatta ctacgtgata agaaattgct tgtttcaatc ctttctatcg caatagcaag
      301 gtggatagct aaagtttgaa attcataatc tgtaaatttc aattgtgtct tattcatgta
      361 tcgattaagc aattcggcta cttttttcaa tatgtcctta tttaagaaat tattcgcttc
      421 accaatgaaa ttgatattca agtctaagag atctgtttta ctgttagatg ctgtaaagcc
      481 accccaataa tagttcacaa cttctgacat caaccttctt ttactttttt catcagcttt
      541 gatgcgcacg ccatttcgat ctgaaactaa ggacatccca tgcttgttta gtctttttct
      601 aacatctttc attgtgcttt ctaccgtcga cctgctcacg tataattcgt cagcaagatt
      661 ttgaatcttt agagaatcat tagtattcaa taatttagca aacacataag ttagacggtc
      721 ctccggagag tctgcgatat tgattttatg actggataat aaatttcgaa gtgtttcttt
      781 ttttccaata agcgaaacgc cttttttagg ttgaaccaac atttgtacat ctggcatttt
      841 ttggattatt tctttcaaaa ttaaaatatc tttgctaatt gttttcgtag atacatataa
      901 ttgttctgat aattctttgt aagttgtgtg ctcattttca atcaaaaagt atagtatttt
      961 catttgccgt tcgctaaatt cgatgcccat tatcttcacc tcctgaatac agcttaagtg
     1021 tacaattatt atctctattt tcatacagaa gaaagttctt gattaatgta gaagtttcta
     1081 atattcttga ttatttcaaa tcaataaaaa agcctgcaat atttatatca caggcttttt
     1141 tctctattaa atatatataa gataatacat ttttctacta gttctcacta atcttgtata
     1201 attaaaccct ccgcaacttg acggagatgg agaagatgcc cccgtctaat ctagcggtca
     1261 tctctcctcc cattccttcc accaattttt ttgacaggta aagcccgatt ccgctagatt
     1321 ccgtatttgt gcggctggta ttttcggtat aaaaacgctg gaagactttt tcaacagagg
     1381 tttgcgttcc tggtttgata ggattggcca ctgttaatcg aatgaatgga tcctcttttg
     1441 aaaatgtcac ctttgcttgt ccatctgcat aacgcaataa attctgtatg atattttgca
     1501 gcactcgcag caacatgagc ggatctgcta atacagttgt atttatcgag tgttcagcga
     1561 acgctacctc gatatttttc tcagaaaatt gttcataaaa agagaaaaat acttcttcca
     1621 cctctttaga aaaagaaagc gtatggagat ccatttcttt ttgcttcgtc tctattcgag
     1681 caagatcata aaactgatcc gtcatactga gtaatcttgc aacagattgt ttgatgatcg
     1741 ttaaatactt ctcccgtttc tccgtatcct ctacctgttc cagtaattgt acataaccgt
     1801 taatagcagt caaaggagtt ctgagatcat gagaaaggcc gacgatcgat agtctgatgt
     1861 tttcttccat ttcttgaaat gcttgtcctt tttgttcatt ttcatccaac catgtattca
     1921 gtgcatctac taggtccatt agattctttt ctcgaaattc gactgaaaga cgaccaccat
     1981 attcttgtcg ttcagaaaga gtggcgattt tcttagttaa tctcttgatt tccctttgca
     2041 ggaagaaaat aaaacttccc ataaagataa caacgagaag caacagccat ttaatcaaat
     2101 ctaaacccca ctccccacac cgtatgaata tacacagcat gtgcctttgc tagcttccct
     2161 cgaagattgg aaatatgcat attcactgtt ttctcactat caaaatatgg ttcttgccaa
     2221 actgtttcat aaagatttgc tcgcgaaaat acttttttcg gatgttctaa aaaaagcttc
     2281 agcagatcga attctcttcc tgtcaaatgg acagccacac cattcacatt tacatccctc
     2341 gactctttgt ccagtaagat ttccttgtaa tagatgcgtt gcgacagatc ttttacttgg
     2401 acagaagtct gatgacgaag ttggatgccg attcgtaaca aaagttcttc gatatcaaat
     2461 ggtttcgtca catagtcatc cgctcctgct gccaacaagg aaactttatc tctttgatcg
     2521 ctttttgctg aaagaacgat gatcggaaca ttcgatgtct gtcggatttc tgctaacaat
     2581 gcttcacctg aaagccctgg aagcatgaga tctagaagga tcaaatcgac aggatatgtg
     2641 cttaacaata attttccttc cgtacctgaa taagcatcaa gaagtttgta tctctgttca
     2701 agaacctcct ccagtaagga atgaacagct tcatcgtctt cgataatcaa aatagtttcc
     2761 agcaggacca cctacttaat gtcagatttt tggaaaataa acattcctag agctgatgat
     2821 accacgattg ttactagact gaccagaatc attttcggtg cttgcgaata agttgcttgt
     2881 tgcgcaaagt ccgtatacat gagccacgtt tccgtaaacc actccgttag aaattccaag
     2941 cctttgattt ttgcgaaaac accgggaatg aaggccgttc ccatgatata aagaaaagaa
     3001 agaagcaaac tgacactggt acttctagtg atgaatgtca aagccactgg aacagataat
     3061 gtccctaatg tcagcagaag caatacagga aatactttta gaacataatt tccatagttt
     3121 ttgattccct caacttcacc aaatccgaaa gcaagtgtgt aagcaagcat ggctagtccc
     3181 gtgatcaaaa ccacgccaag tgttgtgatc aaaagcgtaa cgagaagttt cgacacataa
     3241 taagtcattc gattccgtcc taaagataac gtattgcgaa tcgtaccgtt ttgaaattct
     3301 tccccaatat aaaagcacgc aaatgctgcg aaagccagaa gaatccatgc agccattttt
     3361 tctaccatta aaatccctgt tgcaccgttg atgactgtat ccgttgcttg gtttaattca
     3421 ttgccgtatt ttgtatgcat ataccattcg ttccaagaat aaaaaagcgg aagtacgacc
     3481 atcagtccta gtgagaccca cattttacgg ctcttccata cttttatttt ttcagcagtt
     3541 aatagattaa acatgtggat tgcctccagt taattgtaag aaataatctt ccagtttttg
     3601 cccaactagg tgaataccat ccacaggaac ttttgcttct accaatgctt gattgatggc
     3661 tgctacttgg tcaattcctt caaaaagctg gataacacgg ctagattgta caaaataatc
     3721 cctataacca agagagtcga gaaccgttac tgcttgttcc gtcgccgaag tgtcaatttt
     3781 tataaattga cgagattctt gtgccaattc ttctttcgat aattctttga tcaagtgtcc
     3841 ttcatgcaaa ataccatagt gagtggcgat ctgtgacaat tcatctagca agtggctaga
     3901 aagcaagacg gtgatgcccc gttcttttac caaacgttgg atgatttctc gcatttctgc
     3961 catccctgct ggatccatcc cattcgtcgg ttcatccaaa atcaaaaatt ctggattcgt
     4021 gatcagtgca tttgcaatag ctaaacgctg gcgcatacct aaagagaaat tctttgcttt
     4081 ctttttccct gtattttcca atcgcatcat tttcagcagg tcttcgattt ctctatccga
     4141 cacaccgccg ttcgctgctt ggatacgtag gttatctctt gcagttaatt ctggataaag
     4201 agcaggggtt tcaatcgatt gacccatacg tcgtcttgct gcctgcaaat ccgtcgttcc
     4261 aaataattcg atcgaacctt catcaatcga gatcaaaccc ataatagttc tcataaaggt
     4321 agacttaccc gctccatttt ccccaatcaa tccatagatc attccacgtt tgatttcgat
     4381 tgatacttta tctaatgctt tggcagcacc gtattttttg gttataccag ttgctttcaa
     4441 gaccgtttca tttccaaaat ccatacttat ccttctttct tattgatgag tgaagtatag
     4501 ttgacgggta taaagaaagc agccagaaat gtataaagaa agtgtaaatt ttccaattga
     4561 atgtcaaaaa aaacaatcgg acaatacgtg tctttcctat tatcaggcag tttgttatac
     4621 gcttgtatca gtttcctgat tctttctttt cgaccaaagt cgtttctctg tattcctctt
     4681 cactgaaaat ttttgcttct ttcagatcaa gcttgagtaa ttttaccaga ccggttataa
     4741 ggataactat agcaagactc cctcccagaa aaattagact gtctcgcaga cgtggttgcg
     4801 ttgtcttcca atcaaagagc gcaccaaaca gtgctaatcc aagtggactg gccaccatgg
     4861 taatcgttgt tatcgttgac ataagtcgtc cttgtatatg cgttggaact tctcgctgaa
     4921 gccagaccat ataaaaagca aaagaaaaca ttaatcctta aaaacggact tatgaaaaat
     4981 aatacatgcc gtttgggatt tacaattagc taccaaataa gaggttatga caaaagactt
     5041 ttcacaatcc cttcttccga ataaacggtg ttctcctgcc aactcctcgg tcacaagtca
     5101 caatatttat caaacatgag aagcaatttt tgtaaattcc ttcttatttc ttgaagctga
     5161 ccacttctgt cacaacctca gaagcggata aataatattg tttgatgata ctttatcttt
     5221 ttcccgtttg tttatagatc gaatgatatt atagaccaag aatcctacct attaacatag
     5281 ctaaaaacac aaaaaaggca ctttccaaat tgtggaaagt gccagaaaag ttaggattaa
     5341 ggtaactgcg ctcagcattg cttcgcccag tacgattctc gtaactcgtg aactcgaacg
     5401 ataatcgcaa ttaacgtttt gagaactgtg aagctttacg ggctttctta agacctggtt
     5461 ttttcttcgt atcattgaat aagtttgaaa aaccttgatt tataaaggtt ttcccgtaaa
     5521 tatattttat tatatttgat taaatttgaa atgtttgtgt tctaaaatgt gttctaattt
     5581 aaaggcagta tatttcgtat tttagattaa atttttaggc tcattcattc tcaaatccat
     5641 tctattcagt caataaaaat tttaaaaaga ctttataaac aaagatgttc aagcgagcat
     5701 cttatttttt tgtgcttaat ttgaaaggag aacatttcat gtataaaaaa caaaaaaaga
     5761 aattatgtgt attaactatt ttgagtttgg ctgttcaagc aattgttgga gcaattgctc
     5821 ctacgattac ttttgctgat gaaattaccc atccacaaac ggtaaccgta gaactagatt
     5881 tagcacatca atatgctgtt gaaggaactt ttagtgatgg tcgtccaatg tcagaagtca
     5941 ctgttccgca ttatgcagtg tacaacggct ttaaacaaga tattttctgt attgaaccag
     6001 gagtcccgat ttacaatgag ttcactcctg gctatgagaa aaacccgtta ccagatatgt
     6061 ccgataaagc caaattagtt tccgttttat ggaaaaaagt aggtacagat gcagatactc
     6121 atattgtggc acagaagatg atctggcaag aagttaatgg gtataccctt cactcaataa
     6181 aacgatcaga tggtagtgcc gtaaatatcg cagccattga ggcaaaaata aatcaagcca
     6241 ttgacgatta tcagaagaaa ccaaccttcc ataatagtac ggctaaaaca gtattagggc
     6301 aatcgactac tgtgacggat acgaataatc tgaatttgtc agagttcgat gaggttgtgg
     6361 aaaatgccgc aaacattgat tatcgtgtga atggtaatca gttgataatc accccaaatg
     6421 ccaattcaaa agaaagtggt gtgctgacgc ttaagaaatc tgctggtacc ggaacacctg
     6481 tcgcctataa aaaggctggc caacagactt taatggccgg ggcgattgat aaacctaata
     6541 cctacacaat caaaattgat gtggaaacgg agggttcttt aaagatcaaa aaagtcgata
     6601 aagaatcggg tgatgttgta ccaggaacgg ttttccattt agactttgga aaaactttac
     6661 ctgcaaaaga cgtgactact gacaaagaag gcattgctac attggatgag attcctcatg
     6721 gtacaaaagt aaccattacc gaaaaatcag taccggcgcc ttatacgatt gataccaccc
     6781 ccatgactac caccattaaa gcaggcgaaa ccatttacgt gacttcaaaa aacgcacggg
     6841 aaaaagggca aatcattctg gataaaagcg gagttgaaac agggagtgat ctttggaatg
     6901 acaattattc cttagctggt aatacgtttg ccattcgcaa agacagtcca accggtgaaa
     6961 tcgttcaaga aatgaccacg gatgaaaacg gtcatgcgga aacaccaaaa gagattgcta
     7021 atgcgttgga attgggaacc tattatgtga ccgaaactaa agctagtcat gggttcgtga
     7081 atacgttcaa acctgtaaaa gtcgaattga agtatgccaa tcaaaccgta gcccctgtta
     7141 ccagtaacgt aaaagggcaa aatcaagaag tcactggcga aaccactttg acgaaagaag
     7201 acaaagatac tggggataaa gcacaaggta aggcagtctt tgagggtgct gaatacacct
     7261 tgtttactgc taaggatgga aaagctgtta aatggagtga ggcttttaaa ccagagttag
     7321 cgaagggaac gaaaggttct gatgaaacgg tcactttgac tttagacgaa aagaaccaag
     7381 ttgccgttaa acatttagct atcaacgagt attattggca agaaacaaaa gcgcccgaag
     7441 gatatagcct tgatgaaaca aagtatcctg tatccatcaa aaaggtggat gatagcgaaa
     7501 aaaatgccgt gattacccga aatgttacgg caaaagaaca agtgattcgc tttggctttg
     7561 atttctttaa atttgctgga tcagctgcgg gcactaccga aactggcttc aatgatctga
     7621 cctttaaagt gtcgccattg gaagggacca atgaaatcac aggtgccgaa gatgaagcga
     7681 ccacagctta taacgagcaa ttagggtttg atggttatgg caagtttgaa aatcttcctt
     7741 atggggatta tttacttgaa gaagtagaag ccccagaagg ttttcaaaag attacgccat
     7801 tagaaatccg ttctactttt aaggaaaaca aagaagactt tgtgaagagt gagtatgtct
     7861 ttaccattac cgaacaagac caaaaacaac caattaaaac ggtgaccgtt ccttacgaga
     7921 agctgacgaa caaagcattt tctgttagtt tgaaccgttt aatgctctat gatttgcctg
     7981 aggaagaaga tagtttaact tctcttgcga cttggaaaga cggaaacaaa gaattaactt
     8041 ctctggattc taccgagcta gttgataaat tgagttacaa tctacatgaa atcaaagaag
     8101 actggtatgt ggtagcgcaa gccattgatg tggatgcgac aaaagctgcc caagaaaaag
     8161 atgaaaaagc caaaccagtg gtgattgcag aaacaagcgc aacgttggcg aacaaagaga
     8221 aaacaggaac ttggaaaatt cagcataaat taaccgccga acaagtttta aataaaacta
     8281 ttgtcttatt caactatgtg tatgaaaata aggaagcttt tgaagcaggc gataagccag
     8341 tagcgaagga tgttagcttg aataaccaag cccaaactgt cagttgtaca gtggaacacc
     8401 atgtttctat ccaaacaaag gcccacctag aaaacggttc tcagaccttt actcatggtg
     8461 atgtggtgga tatgtttgat gatgtgtcga ttactcatga tgtactggat ggctcaaaag
     8521 aagctttcga aacaattctg tatgctttat taccggatgg tacaaacaaa gaaatttgga
     8581 aatctggcaa gattgattat gaagtaaatg ataaagaatt caccaaaact gtgcttgcga
     8641 aaaaagtaga taccggaaaa tatccagaag gaaccaagtt tacttttgcg gaaatcaatt
     8701 acgataaaga tgggaatatc aatggaaaac acaatgaaga tttgaaagaa aaatctcaaa
     8761 ccttaacacc aaaagaagtt ccaaccatac taagtacgcc gaaacaaccg gaaacaccaa
     8821 ctgttccaag tggctctcaa gaatctagtc ccacagtgaa gaccttccct caaaccgggg
     8881 agaaaaattc caatgtttta ctgttcattg gttttacttt gatttttgcg acggcgggct
     8941 attatttctg gaatcgccgg aactaaggtg atgagatgat acaagtaaat cctaagaacc
     9001 aacgtgcgcg accgcctcct aaaaaagtag tcgcgctatt tttgtatcca aaactaacaa
     9061 tgaaaagagg aaaagaagat ggaattgaaa tttgtcgtgc cagatatggc tgaaaccttt
     9121 ggaaagttaa gttatgccgg tgaaggagaa attataacag aaggatatgg acggaatact
     9181 acggtgattg gtcgcagtta tcatttgtat tccagtaagc aacgggccga tgatattgag
     9241 gtggtagtag ctgcggaagc cggtgaaaag gattttgatc aagaccaacc gctaaaagcc
     9301 gtgaatcccc atttggttgc caaaggctat gagattgaga atcgtgggtt tactgattac
     9361 gtgttgtatg ttgatgattt agtgaaagca taggaggaaa agaagatgag attaacagaa
     9421 ggtattgtcg tagattcagg tttaacattt gggaagttgc gtttttccgc attacgccga
     9481 gaagtacgga agcaaaatga ggatggaaca attggtaatg aggtaaaaga acgaacctat
     9541 aatttaaaat cctctgcaca agggcgaatg attcaagtta gtatcccagc taatgtaccc
     9601 ttacgcgagt ttgcttatga tgcagaggta gagttggtca atccaatcgt ggataccgtt
     9661 gccaattatg tttttcgaga aggaactacc gttaattggt tcattaaggc ggatgattta
     9721 gtcttgaagc ggcagccgaa tcaaggaaat cctacgaacc aaaatgaaag aaagaaatag
     9781 gtgaagatta tggaaattaa tgttgaacat attttagatt gtctcgatca atatggaaaa
     9841 ggcgagctga ctgaggaaca actcgttaaa gcattaacct ctgatgaaaa aatgtttctg
     9901 attatacatc aaggtttact tggagaaaat agcgacagtg aagaagactc tgatgtacta
     9961 gactggttag gagaagaaga gtctttcttt atagttgtcg aagtaaatga aaacttatgt
    10021 cggcaggcag aatccatttt ggaagaaatt ggtgtggaga tgccggatgc tattgaaggc
    10081 tttttaaatc aattggttga aacgaaacaa ctcccctcct cgtagcagta aatgactagc
    10141 ataacttttt gaaattgcct aatgaaatcc tattgggctt tttcgattgg aggtggaaac
    10201 catcaaaatg tacaaaggac atcgcatacg cgcaggagat cagcacttgg tttatcactt
    10261 tgttcttggc tggctactcg ctctgttcat cggttggatg agcgtctttt attttcaaga
    10321 gtttagacaa tttgatattt ctaagctatc actttctact attgaaatcg tttggtctat
    10381 aaaagattta gtatgtctgc ttggaagtct cgctttttca ggagctatga tattgcttta
    10441 tattcacttt tttcttgatc attggcgaag cttgtggcat cgacaaaagc tagctcggat
    10501 gattctagag aaccattggt atgaagtgaa acaaactcaa agtgaaggct ttttcaaaga
    10561 tttgaatagt agccgaacca gagagactat cagttacttc cccaaaatct gttatcgaat
    10621 gaaagatgga ttactgtcta ttcgcgttca aatttcactt gggaaatacc aagatcagct
    10681 tttaaaattg gaaaagaagt tagaaagcgg actgtattgt gagttagtag aaaaggaact
    10741 caaagactcc tatgtggagt atactttact gtatgatatg atcgccaatc gaattgggat
    10801 agacgaagta gtggcagaaa atgggactct gcgattgatg aaaaatcaag tatgggctta
    10861 tgattcttta ccccatatgt taatcgctgg tggcacgggt ggagggaaga cctatttcct
    10921 cctcaccatt atcgaagcac tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa
    10981 aaatgcagat ttagctgatt taggtacggt gatgcctcat gtttattcac aaaaggaaga
    11041 aatttctgct tgtgtggaag atttctatga acgtatgatg gctcgtagca aggcaatgaa
    11101 agaaatgtct aactacaaaa caggagagaa ttatgcctat cttggacttc cacccaactt
    11161 tttaatcttt gatgaatacg tggcttatat ggagatgtta acgacgaaag aaagtgcggt
    11221 gattttgaat aagctcaaac aaatcgtgat gttgggtcgt caatctggtt tctttctgat
    11281 tttggcttgc caaaggccgg atgcgaaata tttaggagac ggaatccgcg atcaattcaa
    11341 ctttcgcgtg gctttgggtc gtatgagtga actcggatat tccatgatgt ttggggaggt
    11401 cgaaaaaaac ttctttatga agcacatcaa aggtcgaggt tatgtggata caggtggcag
    11461 tgtgattagt gagttttata cgccacttgt accaaaaggg tatgatttct tagattcaat
    11521 tagtaaatta gaaaagatga atgattgagt gatcttcaat actgacttta tacttcaagt
    11581 aacgtataat acttttatat agtaggaaga aaggggataa ttaatatgga aagtaatgat
    11641 tcttatttag agtatgcaaa agcaaataag gataagttta tttcttatat tatcaaaggg
    11701 aaatcccccg aagttggacg agacgcagtt tttatggcag ggagtcctgg ggctggaaaa
    11761 tcagaggttg ccattggatt agctgagaat tatgataacc atgtgattat tgatgcggat
    11821 tactttcgca cccagtttcc tgattataat ggaaaaaact ctagtctctt tcaaaaagca
    11881 tcttcttggt tggtagagca ggcatttaga tatgtcttag atcatggcta ttcttttatt
    11941 ctggatgcaa cttttgcaat attaagtgct gaaaaaaata tctcgcgggc gttgaaaaaa
    12001 cattttcgag tgacgatttt ctatgtttat caagacccta aaatcgcttg gaaatttaca
    12061 caagaacgtg aaattgcaga gggacgtcat gtgccgaaaa aggcatttat taacgctttt
    12121 ttcaaatcaa gagagaacat tgcaaaagta aagagtaaac atccagatgt attgcttcat
    12181 atcataataa aagattatac gaataatatt tccgaagttc attatgctac ggataatcta
    12241 gaattatttt taccactagg gtatagtagg aaagatttgg aggttcaatt acatgactga
    12301 gaaagaacaa gtaacgaaaa ttgtaaagaa atacaataag agcattgcgg atttatcgga
    12361 aaatgctact gcaaaagagt tcaaaacggt gattaaatat gtagccgatc aggccaatga
    12421 aaaacaacgg aaattagttg gattaaataa aaaatagtag gacaaattaa taatgtatga
    12481 gccaagagca aaatgttctt ggcttttttc tttgcaatga atgaggtaat gtatgagatt
    12541 tttagattta ttcgccggta ttggtggctt tcgtttaggt atggaacagg cgagccatca
    12601 ttgtatcggt ttttgtgaaa ttgataaatt tgctagacgt agttataaag caattcatga
    12661 tacaagtaag gaggtggaaa tgcatgacat cacaagtgta tcagatgagt ttattcaatc
    12721 cctcggacca gtggacatcc tttgcggcgg atttccgtgc caagcttttt caattgcggg
    12781 aaagcggcaa agattttccg atactcgagg tactttattc tttgaaatcg ctcggttcac
    12841 cactcttctc cgacctaagc ttttattcct tgagaacgtc cgaggattgc tcaatcacga
    12901 aggaggggct acgttcgaga cgatcctccg aacgctggat ggattggggt atgacgtgga
    12961 gtggtaggtg cttaactcaa aggcctacat tccccaaagc cgggagcgga tattccttat
    13021 cggacattct cgagacgcat gtaccgaaca agtatttcct atcattggac cgtcttcaac
    13081 atctgatcaa aacatcagaa atttggtaaa tatcaatcct tccaatcgag gaatgggtgg
    13141 acaagtctat ggttcagatg gcgtagcgcc aaccttaaca ggtgatgaag gaatcaaaat
    13201 cgcactgcca gcaagggatg gaatctctgt agcaggacta ttaccgggaa agtttgaaca
    13261 aggtaatcga gtttatgaag ttactggaac cgcccccacc ttgtcaacga aacaaggtgg
    13321 tacaaaaatc atgattcgaa aaaatgaaac agaatatcaa gaggtgaagc cagaagacag
    13381 tgtgaatctg gcttttccta attccacaag tcgccgaggt cgtctgggaa aacagagtgt
    13441 tcatacctta ctaacaggag accaacaagc gatcgtcacg gatcagtatc aaattcggaa
    13501 gctaacacct agagagtgtt ggcgacttca gggctttccc gattgggctt ttgatcgaga
    13561 ttctcaagtc aattcagata gtcagttgta taagcaagcc ggaaactctg taacagttcc
    13621 ggtcattttt gatattgcca gaagattaaa agagttggat gtacatggtt tatgatgagt
    13681 tgattggaga aatttattgg gtgattggaa agattcagtc agacccagaa ttagaagaac
    13741 aattgcgacg gttaaaattt gatattcgca agaatggtgt caaagtcgct ggcgatccat
    13801 atttaatgaa tgaggagacg gatgctcgaa ttgaaataaa tcaagtaatt gcagaatttg
    13861 agcggattgc ggaccaagca aaagaaccag atattcgtca atacttgtta gaaatgaaag
    13921 cagagttgga agtaaatggt attacagatg agtaacctaa accaacacaa ctgactgtta
    13981 ggcgctgacc gcggaggatt cgcgaagcag aaatcctccg tggtgctgtg cctgttcatg
    14041 gacggcgtta gccgaccatg atgtctcacc cccgtatcta acaggggggt acaaaaacac
    14101 aaaaaataag taagcaacca ttaagaatgc ttgttgtatc aaagatagta agtaatttgt
    14161 ataggtgtta actcatttga aaaagttaac accttgaatt tggaggtgag aacttggcac
    14221 aacggaattt agattatcgg ctattaaaag accgacgcaa cgaatacggc gtttcacaaa
    14281 ataaattggc tacggcttgt ggacttagtc ggccgtactt aaatcagata gaaaatggtg
    14341 gcgtaaccgc atctataaaa accatgagga aaatttttga ccaactggaa agctttaatc
    14401 ccgatttgcc tttgacgtta ctgtttgact atgtaaggat tcgctttccc acaacggatg
    14461 cacgaaaaat tattcaagaa attctccatt tgaaatttga gtatatgctc catgaagatt
    14521 atgcctttta ctcctatcaa gaacagtatg tcatgggtga tattgtggtg atgttgtctc
    14581 atgaagaaga taaaggtgtg cttttagaat taaaggggcg aggttgccga caatttgaaa
    14641 cttttttact cgctcaaaag cgtagctggt acgacttttt cgaggattgt ctaaaggccg
    14701 gtggcgtgat gaaacgcttg gacttggcaa tcaatgatcg agtggggcta ttggatatac
    14761 ctgagctaac gaaaaaatgt cagaaggaag aatgtatctc cttgtttcgt acctttaaaa
    14821 gttatcgttc cggggaactt ttaaaggctg atgaaaagga cggcatgggc aataccttat
    14881 atatcggcag tctcaagagt gaggtttatt tctgtttgta tgaaaaagac tatgaacaat
    14941 atatcaagtt agggattcca ttagaccaaa ctgagacgaa aaatcgtttt gagattcgac
    15001 tgaaaaatga ccgtgctttt catgcgattc aagatttgtt aaaaggtcgt agcattgaga
    15061 gcaccacgtt ttccattatc aatcgttact tgcgatttgc ggataaagta gaaggtaaaa
    15121 ggcgaaccaa ctggcccttg aatgaacaat gggggcgttt tattggacgg aatcggaaag
    15181 agattcaatt aacttccgag ccaaaaccgt ataccattga acgaaccttg aattggctag
    15241 gccgacaagt cgcccctacg tggaaaatgg ctaaggagtt ggatcgattg aatcggacaa
    15301 cctatatcca ggatatggta cagaatgcac gcttatcaga ccgacataaa aagattttgg
    15361 agcaacaaag tatggcaatt gaaaatttga ttatatgaaa agaggaatcg tcatgggaat
    15421 tgtaaaagat atttttttgg tatttggtgg cagtatgctt ggtgttactg tgatgtgctt
    15481 gatgaatgca agtgcagcag cggatcgagc aatggaaaag aaaggaaagt gacaagatga
    15541 atttcggaca aaattt
//
LOCUS       DACDTO010000068        15310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDTO010000068 DACDTO010000000
VERSION     DACDTO010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.068"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..498)
                     /locus_tag="IWK70_002091"
     CDS             complement(<1..498)
                     /locus_tag="IWK70_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9614604.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGS"
     gene            complement(685..1335)
                     /locus_tag="IWK70_002092"
     CDS             complement(685..1335)
                     /locus_tag="IWK70_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614605.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1878..2813)
                     /gene="whiA"
                     /locus_tag="IWK70_002093"
     CDS             complement(1878..2813)
                     /gene="whiA"
                     /locus_tag="IWK70_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9614606.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2847..3845)
                     /locus_tag="IWK70_002094"
     CDS             complement(2847..3845)
                     /locus_tag="IWK70_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9614607.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3842..4726)
                     /gene="rapZ"
                     /locus_tag="IWK70_002095"
     CDS             complement(3842..4726)
                     /gene="rapZ"
                     /locus_tag="IWK70_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9614608.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4861..5592)
                     /locus_tag="IWK70_002096"
     CDS             complement(4861..5592)
                     /locus_tag="IWK70_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9614609.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5596..6861)
                     /locus_tag="IWK70_002097"
     CDS             complement(5596..6861)
                     /locus_tag="IWK70_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9614610.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7124..9940)
                     /gene="uvrA"
                     /locus_tag="IWK70_002098"
     CDS             complement(7124..9940)
                     /gene="uvrA"
                     /locus_tag="IWK70_002098"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9614611.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(9950..11944)
                     /gene="uvrB"
                     /locus_tag="IWK70_002099"
     CDS             complement(9950..11944)
                     /gene="uvrB"
                     /locus_tag="IWK70_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9614612.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12203..13705
                     /locus_tag="IWK70_002100"
     CDS             12203..13705
                     /locus_tag="IWK70_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9614613.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13707..14444
                     /locus_tag="IWK70_002101"
     CDS             13707..14444
                     /locus_tag="IWK70_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9614614.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14489..14662)
                     /locus_tag="IWK70_002102"
     CDS             complement(14489..14662)
                     /locus_tag="IWK70_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9614615.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14638..15303
                     /locus_tag="IWK70_002103"
     CDS             14638..15303
                     /locus_tag="IWK70_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9614616.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
BASE COUNT         4493 a         3278 c         2592 g         4947 t
ORIGIN      
        1 tgatccttct tttatagatt caggaaacag tataatttta gtactagata aattgtttac
       61 accgattagt gttaaaatat ctttattctt cttgctaaca taacccttta tgcacaaaag
      121 aacagctata cttaaagtaa caaataaata acgtattcca atagaaaaat cactataaat
      181 actctctagc ataggaccga caattgaaga agtcactact gcaaatgctg gacctatcca
      241 agcaacatta gaatgttctt tctctctttt cctttgtaaa tctttgttga gtattaatct
      301 atttatttct tctgcaggca agttaatttt cataacctta tgggggatta tccaattaaa
      361 aatcggaaaa aggtagcctc cccaaaaagt atagtgatct gtgtcaatca gataataacc
      421 tgattttgat tccagaataa catatctcaa agccgaacga aaatttttca tgaatatcca
      481 taatctcact gtttccacac ctaattcctc caattccaat taaatggatt tatagcatca
      541 ccaattatgt taactgcctg accaacctca ttgataaaat tggcaccact atcccatgct
      601 tcaacaatgc tatatatcta tcaaacaaaa aatggaaaac taaatactcg cgcacataaa
      661 ttttacattc tgttttttat aagactaata ataatcattg aaaaaatttc ttatcggtgg
      721 cgcatattcc caattgatta atgtgatccc atattctgca agtattttac gagcttcttc
      781 taagtcatag tgagaaaaat gcataaaacc tgcaatacga tcacggactg tgagctgtgg
      841 cttgcttcca ccatcattca taaaatatcc agcagagcca ctacttaaag tgattgttac
      901 atcttcactt tctattatta agaattcata atgaaatgct ttcaactctc cactattatt
      961 taaaagtttt atactccaac ttcgtctagg ttgagttgtc acattccctc taaaacttcc
     1021 tataaattct gcaatcaccg tatcttcaat caaaattctt tcctcatcta tttcatattc
     1081 aagactaaac ggaaattctc catatgtaat ctctggcaat ggtggtgctg tagttctcca
     1141 aataaataca ataactgtga atagaaaaaa tccaacgatc gatataaatg ttatgagacc
     1201 tgctcgaagt aaaacccgat ataataaaaa tgctgtattt cgctttgcta tatctagaaa
     1261 aataaaaagt actgcaatca taatgatagt aaaagttgta aaaaagcaca ttttttgccc
     1321 tccctttata aacatcgatt ctcaaattct aaaccaaatc ttgatagttc aagttcaggt
     1381 ataacgtata ccaaccatac atcccaatta ctatttatga acttatgatt atcactgaat
     1441 aaatcactcc atccccttgt taaaacttct acattaatta cacctggttg ttttcgacta
     1501 gctaatccaa tgaacttatc aaccatcctt atccaccaac cacgagaatc ttgaccagaa
     1561 gtaagaaaaa cagttctcaa ggttgatcga gcagttcctt ccagttttta attaatctcc
     1621 agttatagat aaatacttga tcctatctat atcaggactt ctatattaag caaaagttta
     1681 tgtataaaac taaaatcttc attcagttgt ctccccgctt tttatacgat tgtattaaaa
     1741 aacaatagaa gcctttcaac attatgttac tccagtaaag ttggaattat agtttgacgg
     1801 aagaaaagca agtatagctt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta
     1861 tcagtttttt ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt
     1921 ttgcgaattc ggtgattaat tcccgatttt gatattgcac ctgagggaat catttcccct
     1981 aattctttca agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt
     2041 tcaggaaggg catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta
     2101 gaagccgcat caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc
     2161 gaatttctca tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca
     2221 ccaatcaatg ttaagaaatc agcaattttt tctgctcctt ttaaatacga aatatagcca
     2281 ttgcgccgtt caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct
     2341 tggttatgct gttcataaat agaaaatatt tccaaatgat aacgactcgt ttccggatta
     2401 ttgactgaac ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt
     2461 cccataatct cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc
     2521 atcaacacat cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat
     2581 ttcatttttt tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa
     2641 tagatacgtc tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc
     2701 acaataccta aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct
     2761 cgatgaactt ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc
     2821 tcctcctttc tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat
     2881 tcttctacta ctttgtctcc atcgtggaag acaccaccat ctttcaattc taaaaagtcg
     2941 gtggaaatca ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact
     3001 agatactcat catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc
     3061 aatactgtat cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca
     3121 tctgtgaaat gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca
     3181 gcagttgtct tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg
     3241 gtgaataaac ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact
     3301 tttcgagccg ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg
     3361 gttttacggt tgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaaaaca
     3421 gcatgcaagg tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt
     3481 tttgctaata attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg
     3541 atcagattac cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg
     3601 aaaatatctt catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca
     3661 ccaggaggag ccatagcgat cgattctctg attgcaccgc tgcttccacc atcatctgca
     3721 acagtcacta ctgctgtgat atctgctcct tgatttcgta aacttttcag gataactggc
     3781 agaccagtgc caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt
     3841 ttcacgaccg gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt
     3901 tatagttttt ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac
     3961 cgcctgtaca gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca
     4021 atacagtttc cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca
     4081 tcacataatc atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat
     4141 gagggttggg caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt
     4201 taaaaccaaa tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact
     4261 cattgatttt ttcacgtaat tgtcgtggtg tcaatgttgt cgtatcaatg atcacggaag
     4321 cttgggcttt caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac
     4381 cgtccatcgc cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt
     4441 ctgaagcatc taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca
     4501 gcatgttttg gatttcttca aagaaggaac gagaacgcaa atccactcct aaagccattt
     4561 ttgtgacttt acctgattct ttgatcaatt cccagaattt tggaatcaat cgaggcggca
     4621 tattatcgat acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg
     4681 caccactcat acctgttatg atgactaatt gcaaattatc tgtcatattt acgaacccct
     4741 ctcacattgt gcataataaa attatatcat cccgggcgta tcattaccag ttttcttttt
     4801 cttttacaaa aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt
     4861 ttacttttcc gatgctgtat ccaatgcctc gcttctttca gctaatgcac gttcgatcgt
     4921 tcgatccgct aaaatagatt gagcatcgat cactgggtaa tgattatctt cagcaaattc
     4981 atgcatcaaa gataattctg tgcaacctag gatgactttt tcacagttta ggcgctctac
     5041 cgcttcctct aagatttcgt aatagagctc ttgattcaaa taatccgatt ccttgatctc
     5101 atgatagatc aggtaattga ttttttcttg aagcgcagta tcaggaatca ctgtttcgaa
     5161 accaagattt ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag
     5221 aatcgctact ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat
     5281 atgcaagata ggaatatccg ttgccgcttg taactcttca aaaaagtagt gtgcggtgtt
     5341 acaagtcaac acaatgaagt ttggtcgcaa taaattttgt ttctcaatat catccagcag
     5401 aaacggcatt ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt
     5461 tgcatgattg aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg
     5521 gttgatcaag cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa
     5581 aaaattctcc atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata
     5641 gttatggaac atatatttca tcaacaacca gcgcttgatt gatcggtctt tttcataaaa
     5701 tacagtagta ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt
     5761 ttcccgagca tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc
     5821 tgattgattc gttccatagg tcgtttcaac aaagggtttg ttgaacactc gatcttctgt
     5881 gacaaaacga gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct
     5941 caaattgatt tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt
     6001 ggcaaagcct gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc
     6061 gttataatct ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa
     6121 tagtggatga ccaaggcaca tcatccgaac ttgatgatct tcatctacat acgcatttag
     6181 cacacgcata ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata
     6241 accggcttcg tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa
     6301 agcttttttt cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa
     6361 agcaactggg aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc
     6421 tctgacgatc aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct
     6481 tactttattg atcagccttt caaataaaga ataatcaata tatggacaga taaaatattc
     6541 tgacagctct tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa
     6601 aagatatttt ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat
     6661 aaatacaggg tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg
     6721 ggtaggggct aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa
     6781 agaacgtgcc ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc
     6841 attttcaata ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc
     6901 aactacacaa ggaatatcat accataaaga aaaaagcaat caaaggaaag tcctccgatt
     6961 gctcaattct tttttgaacg gattgaactt ataggaagtt tttatctata ccaatcagtg
     7021 tagaaagaac taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt
     7081 cattttttta tgttttttta actatctcag ctatctatcc tctttatacc attatttttt
     7141 tcaaatagtg acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg
     7201 caaggatcgt tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg
     7261 ttttgattac atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc
     7321 tttgtaatac atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat
     7381 ctagtatata gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc
     7441 gttgtgcctc accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc
     7501 caacatcaac aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta
     7561 cagcatcctc tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt
     7621 ctaatgtttc agaattatat cgtttaccat gacatacttc acaaggaaca tagacatcag
     7681 gcaaaaagtg catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc
     7741 cttttacgtt gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt
     7801 gagcaaataa cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg
     7861 gcgtccggcc gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg
     7921 aaattgtctt gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt
     7981 tctttaagat actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta
     8041 caaattctcc taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag
     8101 tgatcttgat tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt
     8161 tccctgaaag atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc
     8221 ctgcagccac gatttgtccg ccttgttctc cggccccagg tccgacgtca atcaaataat
     8281 ctgctgccca catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac
     8341 gcattttctt taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg
     8401 gttcatctaa aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga
     8461 ttcgctgcgc ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta
     8521 gtcccacatt ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa
     8581 ttactttctc ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag
     8641 acaactcatt tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat
     8701 tcaatcgata acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct
     8761 cacgcgtgaa atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt
     8821 cgaaagtcgt ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa
     8881 gatcatctgt cccattcaag atgatctcct gatgctcttc cggtaattct tcgaatggtg
     8941 tatctaaatc gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag
     9001 agctgattgg attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa
     9061 tcactaaatc ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc
     9121 caaaaggcgc attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat
     9181 aaggacaggc atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag
     9241 catagccgtc tgctaatcgc agggccgcct ctaaagaatc aaataggcga gaacgaatcc
     9301 cttctttcac aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca
     9361 gctcaggcac ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct
     9421 ctttttggat tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg
     9481 ccaacacttg gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta
     9541 ctgattgact ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa
     9601 acaacaagcg taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt
     9661 tacttgttgt tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt
     9721 ccggcttatc catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc
     9781 tccgctgccc ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca
     9841 aacctgtaac gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat
     9901 gggcacgtgc accatgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag
     9961 cttttaattc taaaatcata tctcgcacat tcgcggctgt ttcaaaatcc aaagcttttg
    10021 ccgcatcttt catttctttt tctaatttca tcaataacgt gtctttttct tccttcgtca
    10081 tttcttcgta cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag
    10141 aaatcaaatc tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt
    10201 acttctgctg aatcgtacga cgtcttgacg tttcatccat cgctagacgc atagaatctg
    10261 ttactttgtc tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg
    10321 tttggactaa ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa
    10381 caagggaaac ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat
    10441 caaattcacc taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac
    10501 tatgcaaata tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca
    10561 tttttttcgt caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt
    10621 cgcctactag atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca
    10681 gccctgtcgg acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc
    10741 ctggcgtcgc agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa
    10801 gtggccgatt gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc
    10861 tcgctcggtc accattatac attcctctga tttgaggcat ggttacgtgc gattcgtccg
    10921 ccacgatcaa aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt
    10981 tacgtccgtc catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca
    11041 tcatttcaat atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt
    11101 catttcgcaa gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct
    11161 ccatatgttc atcattcgtc acaaagtgag tcgccgggaa gattgctacg tgttccgttt
    11221 ctcctaaaac ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa
    11281 aaaactccac acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac
    11341 cgcgcactcg aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga
    11401 ctaagtcacg aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt
    11461 gtttttggta ctcaaaaggc gagcctaatc caaaaataca tgaaacggat gcaatgacga
    11521 tcacatcatt tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat
    11581 catttacact tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct
    11641 gataataatc ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga
    11701 attccccgta cagctgacca gccaatgttt tgttatgggc aataatcagt gttggtttat
    11761 tcactttctc aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata
    11821 agatttgtgc ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct
    11881 gatcaccagc cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa
    11941 tcattcttcg tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag
    12001 gaaagcaaag gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt
    12061 tttatcttat tatacatgtt atgttttata acataaaatg ttagaaactc gcattttttt
    12121 cattttttcg agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata
    12181 ttggaaattt aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt
    12241 aatgacagga cttgtgacat ttgcgtttgg tcatgttgca caagctgaca acactacaac
    12301 ggcagagtca agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt
    12361 aattgccagt gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga
    12421 aggcatcgat gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat
    12481 ggactaccgt ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat
    12541 gattgccgga atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta
    12601 ctttgatagc gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga
    12661 agatttgaaa ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag
    12721 cgaccatgaa tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta
    12781 cgacgggtta aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta
    12841 tgcgatcaaa caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata
    12901 tgcatttgcc gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt
    12961 gaaagaatta aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg
    13021 ttcagaaact tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca
    13081 aaacaactat caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc
    13141 ctttgctttg gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg
    13201 cgctcttcgc acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt
    13261 tcttgcgttc tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt
    13321 ccttgctggt gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg
    13381 aggcgggatc aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc
    13441 ttataatcgg acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc
    13501 attcgtcaat cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt
    13561 agtcgaattg ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt
    13621 ttacttgatc attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa
    13681 agttttagaa aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca
    13741 aaaaatacgg cgataacaca gtattaaacg acatcaatgt atccatcaac gaaggagatg
    13801 tcgtctgtat cattggccct tcaggttcag gaaaaagtac attcttgcgt tgcttgaatc
    13861 gtttagaaga accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa
    13921 gcacagatat caacttggtg agacaacata tcggaatggt attccaacac tttaacctat
    13981 tccctcatct ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat
    14041 ctagagaaga agcagagaaa aaagcaatca aattactgca gacagttggt ctagaagata
    14101 aaaaagatat gtatcctgaa atgctttctg gtggacaaaa acagcgtgtg gctattgctc
    14161 gtgctctagc gatgaaccct gacatcatgc tttttgatga gcctacttct gcactcgatc
    14221 ctgaaatggt cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga
    14281 tggttatcgt tacgcacgaa atggggttcg ctaaagaagt ggccaatcgc gtcatgttca
    14341 ttgacgacgg aaacttttta gaagatggaa cgcctcaaca aatctttgaa aatccgcaaa
    14401 atgaacggac aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc
    14461 acggtcgcca tgaagggacc gtgtattttt atcttcttat tttctcacct aatcgtatca
    14521 gccgcatcgc ttgttttcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag
    14581 aaagtaccat cggttcattg atgatatcaa gtacatagtc gataccaccc tcacttaatt
    14641 gagcttgatt tttcttctcc atattatgta gttgtgggac aactttctga caatcagcaa
    14701 ttgtgtcaag aacggatcaa tcctttattg gagttgctcg aaagggtctt ctcttattac
    14761 ggacaggctt tgctcactgt acatagacaa caaatcatta tcctagtcaa tcggatttcc
    14821 agagcctcat tgctatcatt gcttgataaa atccagacaa agtttaataa aatgtatcag
    14881 ttacagctaa actttggaat tgggtcgctt tgttatacag agcaagagac accgcaatct
    14941 ttccttcatg ccaaacaagt ttgcgaatgg atagctttcc atcaatctgt gaacgagatc
    15001 cgcttttttg aagatttaga cctcgggatc gttttaccag caatcccgtc agatcaacga
    15061 acattatatg taaaacgtat tttgaaaagc ctgacagaag aagaagtaca tttgttcaag
    15121 aagactttgg catgcttcag taaaaataac ggaagcatca aaaattgctc agaagaatta
    15181 tttattcata aaaacacctt gcaatatcga ttgaacaagt tccattctct cactggttat
    15241 acccctagaa actatgacga ttatcatatt ctaaagctgg cttttttgtt ggtacaaaca
    15301 tgacaactac
//
LOCUS       DACDTO010000069        15140 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDTO010000069 DACDTO010000000
VERSION     DACDTO010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.069"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWK70_002104"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWK70_002104"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9614617.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWK70_002105"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWK70_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9614618.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWK70_002106"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWK70_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9614619.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWK70_002107"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWK70_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9614620.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWK70_002108"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWK70_002108"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9614621.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWK70_002109"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWK70_002109"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9614622.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWK70_002110"
     CDS             complement(8053..8523)
                     /locus_tag="IWK70_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9614623.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWK70_002111"
     CDS             complement(8569..8994)
                     /locus_tag="IWK70_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9614624.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWK70_002112"
     CDS             complement(9061..9330)
                     /locus_tag="IWK70_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9614625.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWK70_002113"
     CDS             complement(9341..10936)
                     /locus_tag="IWK70_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9614626.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWK70_002114"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWK70_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9614627.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWK70_002115"
     CDS             complement(14489..15049)
                     /locus_tag="IWK70_002115"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9614628.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
BASE COUNT         3908 a         3431 c         2684 g         5117 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact
//
LOCUS       DACDTO010000070        14572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDTO010000070 DACDTO010000000
VERSION     DACDTO010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.070"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..497
                     /locus_tag="IWK70_002116"
     CDS             18..497
                     /locus_tag="IWK70_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9614629.1"
                     /translation="MVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITNDLKLSTSMIHL
                     IDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNKKTKLVSLTKY
                     GRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEYQVSLTRKED"
     gene            590..1752
                     /locus_tag="IWK70_002117"
     CDS             join(590..866,866..1752)
                     /locus_tag="IWK70_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9614630.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2032..3012
                     /locus_tag="IWK70_002118"
     CDS             2032..3012
                     /locus_tag="IWK70_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9614631.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3134..4552
                     /locus_tag="IWK70_002119"
     CDS             3134..4552
                     /locus_tag="IWK70_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9614632.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4623..6092
                     /locus_tag="IWK70_002120"
     CDS             4623..6092
                     /locus_tag="IWK70_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9614633.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6102..6569
                     /locus_tag="IWK70_002121"
     CDS             6102..6569
                     /locus_tag="IWK70_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9614634.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6605..7477
                     /locus_tag="IWK70_002122"
     CDS             6605..7477
                     /locus_tag="IWK70_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9614635.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7558..10092
                     /locus_tag="IWK70_002123"
     CDS             7558..10092
                     /locus_tag="IWK70_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9614636.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10248..11021
                     /locus_tag="IWK70_002124"
     CDS             10248..11021
                     /locus_tag="IWK70_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9614637.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11014..11805
                     /locus_tag="IWK70_002125"
     CDS             11014..11805
                     /locus_tag="IWK70_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9614638.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            11815..12306
                     /locus_tag="IWK70_002126"
     CDS             11815..12306
                     /locus_tag="IWK70_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9614639.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12324..12734
                     /locus_tag="IWK70_002127"
     CDS             12324..12734
                     /locus_tag="IWK70_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9614640.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            12738..14138
                     /locus_tag="IWK70_002128"
     CDS             12738..14138
                     /locus_tag="IWK70_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9614641.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14161..14535
                     /locus_tag="IWK70_002129"
     CDS             14161..14535
                     /locus_tag="IWK70_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9614642.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5082 a         2049 c         2949 g         4492 t
ORIGIN      
        1 tagaaagagg tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca
       61 tcaaagtaaa taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc
      121 taaaactcag tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta
      181 gtattacgga attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc
      241 ccactttaga gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta
      301 aacttgtatc gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg
      361 aagagcttta tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa
      421 ccattaaaca gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga
      481 caagaaagga agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca
      541 gaaaaaataa ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg
      601 tcaacgtaga acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg
      661 aaagccccga gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg
      721 ggtcaagcaa gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca
      781 agtagaacta attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt
      841 aaagcaagcg gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa
      901 tactcaatat cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa
      961 gccaaaccag ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc
     1021 aatcgaaaag cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa
     1081 gtgagtcaac gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca
     1141 ttggctcatt tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta
     1201 gacggaacct tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt
     1261 cgtgttggga agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc
     1321 atcggctatt catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag
     1381 attccgtact cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat
     1441 aaccagacga ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa
     1501 ggctgcccct atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc
     1561 gttcatcaat accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc
     1621 cattggtgga ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt
     1681 cgacagcaga gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc
     1741 gaggcacctt aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg
     1801 ttgtcaaggg cagtcggaac accagcagca agcttacgca ataccttata atttttgtca
     1861 aaaaaagtgt tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg
     1921 atattatata tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat
     1981 gtcgaatagt cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa
     2041 attggaatta gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta
     2101 ttgaatgatc gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa
     2161 aaattagaat actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc
     2221 gtaggtttga tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc
     2281 gagttcgagc tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca
     2341 acaaaagaaa aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta
     2401 ggaacgcaca ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt
     2461 gatagaagtc ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa
     2521 ttagctactc aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat
     2581 agtaagataa agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa
     2641 ttcatgctgg aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat
     2701 gaaataatta agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat
     2761 gatttattgg cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa
     2821 gaggttaaag tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta
     2881 tcaactattc aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat
     2941 atgattaagg gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa
     3001 aatacagcat aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga
     3061 aacgggttga catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca
     3121 ctggaggaag aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca
     3181 acctaaggat gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat
     3241 ttatcatcaa cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt
     3301 agcaactacg aaagattttg ttcattttga agactttggg gaatctcttt taagagggga
     3361 tgacaatgag agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt
     3421 tcacgcattt tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt
     3481 gttgcttcat gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga
     3541 gttagaacca caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga
     3601 tgatgaaaaa gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct
     3661 acaaaccgga cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg
     3721 tgatttttgg gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg
     3781 tgactggtgg tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat
     3841 gagtcaaagt ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc
     3901 ttattatgca ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc
     3961 aacaaaagat caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca
     4021 tgaagtctat caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa
     4081 tgcattcata aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga
     4141 agaagtaacg cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt
     4201 tgatgagaat cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc
     4261 ttacgaatat cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta
     4321 cccttggttt caatatatgg ataaaggact tattagacct atatctttaa agcctaacca
     4381 atcttataaa ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat
     4441 cgctcttaac gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa
     4501 tggatcaatg actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc
     4561 aatcatgtgg caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat
     4621 gaatggacta caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata
     4681 tctcaagcgc tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag
     4741 cgaatatgga aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac
     4801 agttacaagt aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat
     4861 tgacaggagc taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag
     4921 gaaatagact tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag
     4981 cttttattgg ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt
     5041 ttggaatgga tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc
     5101 tgggtattat tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa
     5161 aacgttttgg cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag
     5221 gactagccaa tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat
     5281 ttggattaag cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt
     5341 cttggttttt agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt
     5401 acattttagt cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg
     5461 gtccattggg gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga
     5521 agatgggtgc ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag
     5581 gaacacaaca tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt
     5641 atatccaacc tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt
     5701 atttactctt taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa
     5761 cactggtagg tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc
     5821 cttttgtttg ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg
     5881 taaaagccat tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc
     5941 ttccttatat atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt
     6001 atgataaata taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg
     6061 actcaaaaaa attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa
     6121 aaaatgaaat aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa
     6181 gtgatccagt cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg
     6241 aaacgattgt atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct
     6301 taggaataaa aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg
     6361 agctaaatgg tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg
     6421 atattcttat gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca
     6481 ttgttgtttt tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac
     6541 aaggacaaga attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa
     6601 atgtatggtt tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga
     6661 taaagatttg aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat
     6721 gaatttagtt ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg
     6781 atcgttcggt cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac
     6841 gccaaaactt gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat
     6901 tccaactgct tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa
     6961 tggcagaggc ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc
     7021 tatccaaaac ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt
     7081 aaaacgacac aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga
     7141 gggcttagct gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac
     7201 tgaaaatgac gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac
     7261 aacattgatg ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca
     7321 cttaaaaaag aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc
     7381 cgatgttgac aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg
     7441 tttagcttta gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac
     7501 tatttgtttg gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg
     7561 aaaaaaaaaa tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat
     7621 ttttctgctc aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat
     7681 ttgaatcagt tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg
     7741 ttcaagtata atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata
     7801 tctttgaaat ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc
     7861 ccattaataa taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat
     7921 cagtatgctc ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt
     7981 gcagattatg caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga
     8041 gcctttactg gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac
     8101 ctttttttag atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt
     8161 atggattctc aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt
     8221 cgccttatct tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga
     8281 cgcatcccag ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt
     8341 caattattat ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt
     8401 tcgattgaac tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg
     8461 aggaatgaaa tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca
     8521 atttatttag gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat
     8581 ttaaaaaaaa tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat
     8641 cacatttcaa ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca
     8701 ggttcttctg agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt
     8761 gttaataaaa tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg
     8821 gcggatatca gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt
     8881 ttgctgtcta agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat
     8941 gagatttgga gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata
     9001 ctttttcttg taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg
     9061 cacggtaaaa taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat
     9121 gtttatgagg ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc
     9181 aacaattttt gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga
     9241 tcattggaac aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta
     9301 aacaatgttt ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca
     9361 attagtcatt tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa
     9421 ggattatcac aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa
     9481 aaagttaagg atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat
     9541 tttaacgcat taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt
     9601 tgtgttcttt caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat
     9661 ctaattaatg ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt
     9721 gagttctgct taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt
     9781 cgctttttag attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa
     9841 aaatattacg atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct
     9901 ggaatgattg aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga
     9961 gcgcaactat ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat
    10021 atttactgta ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag
    10081 actattttct aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt
    10141 agacgctcat ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg
    10201 cacagtaatt gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc
    10261 aaacattttt agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa
    10321 tcggaacctc ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc
    10381 aaactggact ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg
    10441 gtggtgcatc agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct
    10501 caactaacca ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg
    10561 tacaactaga tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat
    10621 tcgcacaaaa agaagattat caaaaattag agtggatgca atatttggca tcaattaata
    10681 caagctttgt atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg
    10741 gaagtgcaat caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac
    10801 tattacctgc tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg
    10861 cctatttgtt agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt
    10921 cgttacttgg cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag
    10981 tagcaccagc tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac
    11041 aaacaagtaa ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg
    11101 gaagggttgg gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat
    11161 cctgaaggaa tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat
    11221 acagcgcctt acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct
    11281 ttggaagcag taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat
    11341 agtgtatttg tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg
    11401 gaaggtagtt ttgttggaat tattttgtgg atactggcat caattttatt aaggattact
    11461 gcttatcctt tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa
    11521 acaaaattaa agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca
    11581 ctcattgcca cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca
    11641 atgaccggaa atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta
    11701 gcaattgtgt attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc
    11761 ttagtagttt ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat
    11821 gaaaaaatga aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta
    11881 gcaacacaat ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta
    11941 gccaatgatg acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact
    12001 tcaataatta caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa
    12061 aaagttctta ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta
    12121 ccaataacaa gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt
    12181 ccgacgataa atattactga agaagagaga gaggctttta aagaactaat tagtgatgga
    12241 gttgaagtga cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt
    12301 tattaagaac agaggatgag tggatggagt ttataattac atctcatgga aatattgcca
    12361 ttggatttgt agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa
    12421 cacttggaga tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag
    12481 taaaaaatca agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat
    12541 ttgctaaaag aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag
    12601 gaattttgat tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg
    12661 aaaatctaag aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg
    12721 aagaggatga gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg
    12781 attgcatgcc tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa
    12841 atcctaaacc tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga
    12901 aatttgaaga atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca
    12961 tttacgctgg aagtattgtc caaggaatca atgagtattt agctttgtat actggacata
    13021 atcgcaaagc taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag
    13081 atttaattca ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg
    13141 ataagaatga ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt
    13201 tgatactagg agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt
    13261 ttgaatccga agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat
    13321 ttaacatgat tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt
    13381 cagagtattg tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt
    13441 ggaaatcacc agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg
    13501 gaactaatga aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg
    13561 atcaaaataa ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag
    13621 acaaaactct tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc
    13681 cattttcaag ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta
    13741 aaatcgaaga ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt
    13801 ttgctttaaa attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat
    13861 tgattaataa tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg
    13921 ataaggggct gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa
    13981 ttgttgatga tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt
    14041 atgataaagt cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata
    14101 aactagacta ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa
    14161 gtgaaattaa atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa
    14221 atcttttatg aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa
    14281 gtcggctttt tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag
    14341 tgtagaggag ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat
    14401 tcaattcaag atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg
    14461 gaaaatggaa tccgttactt taatttctac ggaccaaatc atgagattat agagataagc
    14521 caaatgaaga aataaataga taacgaagtg ctctaagttg gtatgaacga aa
//
LOCUS       DACDTO010000071        14503 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDTO010000071 DACDTO010000000
VERSION     DACDTO010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14503
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.071"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            83..1081
                     /locus_tag="IWK70_002130"
     CDS             83..1081
                     /locus_tag="IWK70_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9614643.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            1105..1389
                     /locus_tag="IWK70_002131"
     CDS             1105..1389
                     /locus_tag="IWK70_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614644.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            1470..1868
                     /locus_tag="IWK70_002132"
     CDS             1470..1868
                     /locus_tag="IWK70_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9614645.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            1878..4421
                     /locus_tag="IWK70_002133"
     CDS             1878..4421
                     /locus_tag="IWK70_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP9614646.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            4443..6785
                     /locus_tag="IWK70_002134"
     CDS             4443..6785
                     /locus_tag="IWK70_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614647.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            6801..8975
                     /locus_tag="IWK70_002135"
     CDS             6801..8975
                     /locus_tag="IWK70_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP9614648.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            8965..9387
                     /locus_tag="IWK70_002136"
     CDS             8965..9387
                     /locus_tag="IWK70_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9614649.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            10330..11241
                     /locus_tag="IWK70_002137"
     CDS             10330..11241
                     /locus_tag="IWK70_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614650.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            11728..11994
                     /locus_tag="IWK70_002138"
     CDS             11728..11994
                     /locus_tag="IWK70_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614651.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            12066..12407
                     /locus_tag="IWK70_002139"
     CDS             12066..12407
                     /locus_tag="IWK70_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614652.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            12462..12881
                     /gene="ssb"
                     /locus_tag="IWK70_002140"
     CDS             12462..12881
                     /gene="ssb"
                     /locus_tag="IWK70_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9614653.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            12958..13254
                     /locus_tag="IWK70_002141"
     CDS             12958..13254
                     /locus_tag="IWK70_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614654.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            13269..13700
                     /locus_tag="IWK70_002142"
     CDS             13269..13700
                     /locus_tag="IWK70_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614655.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            13882..14187
                     /locus_tag="IWK70_002143"
     CDS             13882..14187
                     /locus_tag="IWK70_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614656.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
BASE COUNT         5128 a         2242 c         3065 g         4068 t
ORIGIN      
        1 gagggggcaa gtgtggttgt ccacgaagta gtgattgtgg acaaaatacc gaaattattg
       61 ttagaacggt atggcatttc tcatgagtaa tgcaaaattt aatccacaag cgttccaaac
      121 taagccaccg aaacaacaga aggaaaagaa acaaaagaaa cgttttttgt tccaaaaaaa
      181 gaaaacagaa ccgtcagatc gaatgatcca attttcgcaa aaaagtgcca tgagtgtatt
      241 ttcaggcgct tttttttgtt tgtttctgtt aatgggtgtc gtgctcctcc taaactttgg
      301 acgagtggat acattaacgc gtttagcagt gaaaaaacaa gtcaatccag aaacattgtt
      361 aactgaagtc aaccaaacct tgtcttctac ggaacaattg aagtacgagg gacaacattt
      421 tattgaaagg ttatttactt attcgccaga aaaacgaaac gaatgggagg cttccgtcaa
      481 tcattattta gcacaaggat tggaagcgtc tgatctaggc tttgtaacga cggctaccaa
      541 tcgagtagcg aaagatgttc gctttattaa actagaagaa atcgatttga cacaagcaat
      601 gtatcgccta ttttatgagg ttacttttac agaaggagaa cattggaaaa gcagtcaact
      661 ttcttttcca gtcagttatc aaaacaatgt tttaaaagta cttgatggcg ttcaattttt
      721 gaatctttct gagagtaaga ataccaattc tgtgaactat caggaaaatc gattttattc
      781 ttctgggcaa gcggtcactg aaagcgaaga aatcaagctg gaagcatttg tgaaacggtt
      841 ttttgagtta tatgtcaaaa atgatgaaaa attggcattg atttctaacg aaaaagggtt
      901 agaaggcggc gaattgatca atgtacaact ggatcatgca gtaaaaaata aagaaggaaa
      961 ctactttttg caagggaagt atcgtttcta ttttgagaaa ggaaacgagt tccaatcaaa
     1021 cttttcaatg gaagtaaaaa caagtggcga tagttatttt gttactacgt taaatgaata
     1081 aaatgaactt agaggagaat ggaaatgaaa ctttttgatc ttaaatcgtt atttgatgat
     1141 gtatctggac aagtcatttg gctattggca atcgttgcga ttgttctaat ggttggtgct
     1201 tatgcgaccc aaggctttgc gcgggcagcg tttgccatta ttggcgtctt tattttgata
     1261 atgctcgtct tattgttatc gaatgccgtt gatattggtg aatggctaaa gaacattatc
     1321 tttattaagg atagtgaaga gaacgtgggg gtaacgcaaa tgattgtgcc ctggatacaa
     1381 ttatcataag aaaaagacaa actgcaaaag cgatcagaag attagaactt ctggtcgctt
     1441 ctttttttgt aaaaggaggt agagtgaaca tggaatacaa ctatacaaag gagtttaagc
     1501 accctcacaa aatttacggg attaaaggac aaaaaattcc ctggatttct aatggcattc
     1561 gcatggaaca attagtcata ggtggggtat ttctcttcat tcttttgatc ctatttttga
     1621 ttgcgtatgt gaatcaaatt agtttcttgc agacactgtt tcgaaagtcc tggttgatta
     1681 tgatttttgg gattggtgtt ctcgtttgga cattgttttc aatgaaatgg gacaataaac
     1741 actttttcga gtatttaatt gggcgaacgt cctattatca gaaacggaaa caacgctttg
     1801 aacatgaaat gtatgtgccg tttttccatg aaaaagtgac gtattcccta cacaaatgga
     1861 aggagtgagt cgagtaaatg gcgtttttgt ttcttttggt cgtctttatt atcgggattt
     1921 ggcagttcaa aaagaaaaaa acgaagcttc ctcgattaga atttccgata gacgccatta
     1981 tccgaaattt tgttttgacc aaaaatgatg atgtgtgggt aggctatcaa ctttcttccc
     2041 aagtatttcc tttaaatgac attgattttt tcaagaacta tcttcaagat ggcgaaggca
     2101 tacttggaca tgatgaatat gactatcatt ttatcaatat tccacgtcaa tttaacctag
     2161 atacgcacat tgaagaaacc attcgagatt tggtaaaggg agaatgcgca gatcttggga
     2221 aagtgtactt tgaacaggca ggaaagattt taaaagacga agtgcaatta aacgagtatc
     2281 gaacgtatct gtttgtgcgt ttcactacag aaatgaaagt gtctgatccg atcgaatatt
     2341 ttcaattgct taagcatgtt tttaaaaagt ttatctatcg tattacggga caacgagttc
     2401 ctataagtag tgtgttacca acgtatgaaa cattagaaaa ggagctctat acggaattgt
     2461 ccaattataa gccgatagag cgcttatctg cccaagagat tggacaaatt tattattatt
     2521 ttttccatcg atcagaagaa aaattgcctg aaagacagct tacgccaatg gaaatttgcg
     2581 agggcgtcat ttcgaatgaa gcaggataca ttgcgattga acagttgaat caaacgcatt
     2641 attcgacgtt tttaagtcaa acggacgctc ctttagccat gtatgggagt agctttattc
     2701 aaaatcttca ggatagctta tcttttccaa ttgatacgca tattcgactt cgatttgagc
     2761 attcaaaatc tgatgaacga aaaattaata aaatggctgg acggatttat gaacaagaaa
     2821 aagaatatga gaccgttgac ggcattttag attcagacga tgtattaaat tttggtacag
     2881 aacggttaat ggatgtaaaa gataagctga agcgcaaaga aaaacgattg tgttattcta
     2941 ccgtcacgct ggttgtatct gcttcttcaa aagaagaatt ggaagaacga gtcaaagata
     3001 ttgaatttgc gattgagtct acagatttca aattttatcg atcggtagcg gatcagctaa
     3061 cgttgtttaa tcaatgctta attggaagta cgcaacgttt tcgtagttac gagcaggtcg
     3121 ctacgacagg ttttgtggca gattatggct tggacttaaa gaaagaagta ggaaataaga
     3181 aaggatttcc tttaggacgg attgttattg caaaaaagtt taaatctgcg aaagaagcgt
     3241 tagcgctatc tagtaaacta gtatggtatt atcccaattt aacaaagaaa agtattgaag
     3301 gcgcggaaca taccaatgga aatacgttga ttattggtcc accaggtaaa ggaaaatcgg
     3361 tccttgtaaa atatattttt ttatggctga tgtttttagg tcaaaaaatt ctttatattg
     3421 atccgaaaaa tgaaacccaa aagttctttt taaaggcact tgaaaaattc ggacacatac
     3481 cagagtttgt ggctatgtat gaacgaatta attttatttc gttatcttca gatgaaagct
     3541 gtcgagggat gcttgatcct ctattgtttt taccaaaaga agaggcaatt caaaccgcta
     3601 aaaatgtgtt aggaatgtta ggagaagtag ataccaatcg agaaacagcg agccacaaga
     3661 aaacggtgat ccaagacgcc atcgatgtag tttacaccat gccaggtaag cattcgttaa
     3721 gtcaagttat cgaagaaatt cgaaaaagtg acaaagaact agctaaattg attttagggc
     3781 atagcgttgg tttaggaaag attctcattg ggaatgagca cagtacgccg atacgttttg
     3841 attcacaaat caacgtgtta gggacacaag gaatacgcat tccgacacaa aaagagattg
     3901 attcgggtcg attaaatagc gaacaacttg caggaatggc aattatggaa gtcattatga
     3961 agtttacgta tatcttttct acaaacaaag aagaagacgc tgcgattatt tatgacgagg
     4021 caaaagggta tgaagatacc gcacaaggaa gctatttgat tgatgatagc ttgcgaaaag
     4081 gacgtgcgaa taatacggat atttaccttg ttgtgcaagc gtttaaagat tacgatcaag
     4141 aagataaaaa agagttaatt tcttacaaat ttgcgtttcg tccgaaacaa aaagaagcac
     4201 gagagaaact attgtccttc tttgatatgg aagtgaattc agccaattta gaaatgatgg
     4261 aacaattaca aacaggaacc tgtttattcc aggatcattt gggaagaaat caaccgattg
     4321 ccattgatgt gttgtttgat agttggttat tggctgtttc ttctactgaa aatcaagatg
     4381 aaaaaattca agcaatgtta gaaaatgaaa aacaacgata aaacaaaaaa gaggtggttt
     4441 ttttgcgaaa aaaatctctg aaatggtgga cgctcctttt attagggggt ctttttttct
     4501 gtctacaagg ggctactgtt tctgccgatc aaatgaatga tggtgcgggt gtcactgaaa
     4561 ccgatcgttc tgaaggagag aatgccaacg ataaagtaga agtaaaagaa gtaacgactg
     4621 atgtggatca attgtataaa aattacaaaa agaattcgtt tgaattgatg accaatgact
     4681 atgagtcaat gaaccttaaa aaaaagctat cgtacaatgt tgcaggagtg atgaaaaatt
     4741 ttgtttggag tggcgtaaaa atgttaggag aattcaatgc ttcaatggtt gaatttctct
     4801 ttgaattaga tattatttcc gccattcgag aacccattca aaacttaaca caaaaaattg
     4861 cggacaatat gttaagtatt gcaggaacta ttggaatcag tttttcggca attatgatgg
     4921 caatcaaata tgctggagag gctcgaatga aacaagcact ccgtgtcttt cttatgacga
     4981 tccttatttt tactgggtta agcgtattaa aagattctgg gacaaataag aatctttttg
     5041 atgttctttt ttctttagat tctacagtgg aaacggcgtt cgtgaaggtc aatccagtgt
     5101 taacacctga agaagaaaca aattatgaaa caaaagaaac agaagaggta acagcggata
     5161 aaaaagtgga aacggcaggc aaattgatgg cggctcgggt cttttatagt aatgtttatg
     5221 aaccatatct gttgttaaat tatggcacaa ccaatgtaga cacaattcgt gagaagaaag
     5281 tcaaatatgc tggaaaagaa tatgatcgaa tcaatctttt attggacaat gatttaggta
     5341 caaaaatagg ggagaaaatt catgacaaag tcacagagta tgaagcagat acactgaaaa
     5401 acaagaacat acaatattac aatagttgga aaaatacagc atttgccttg ttttattttg
     5461 tcgtgaatct gattcaaacc gttgtttatt ttgtcttaag tatgtttcga ttgattattg
     5521 gaactatgca actattgtta gtaccgatgt tgccgttctt acttttttta ggattgtttc
     5581 ttgtcacaat gaatgtgttt ggtaactatg ccaaagtttt tggtattttg acgtttttaa
     5641 aaggaatgac aggttttgca tgtatccttt tttcttcttt tcttagttac gggttccaat
     5701 taagtagtac acaagacgat ccatgggaaa aaattctctt gatcattgtt tatttgtttg
     5761 cgccgttggg aatttatttg ttccgaggct ttttaggcag tctatttact ggaagaatgg
     5821 gtgttcgagg agcgttagct tttgcgggaa atccatttgg ggcagaagca aaattgagaa
     5881 gggaagccaa acaacggttg aaagaacgca aagaagcgag aaaagcacag aaacaaaaag
     5941 aaaaagaaaa cgcacggaag agacaagaac gtcaacgaaa acgtgggggt ggctcagacg
     6001 ttcgtatgcc acaaacggcg aaacaagcca cacgaagtgc ttttcggcgt gaatccgcac
     6061 aacaacctcg tcataaagca ccaaatcgta tggaacgtat ggcacaaaaa gcaagagact
     6121 tgcatgatca agcgaaacaa gcagaagagc aaagtcaaga acgtatgcaa cgaacagcaa
     6181 tggagaaaca ggcaagaaaa acagccgatt tattacaacg agaattgcaa ggcaatccta
     6241 gtctaagaga aagaagtaga aaaggtgcgt ctcgtagtcc gtatcgcaat cagggttctg
     6301 caatggtggt gtcattagcg aatccaaagc aaaaacacaa gtcttatggc cagaagcata
     6361 cgcctagaac agaattgaac gatcgaaaac gtcgtcaggg acaaacaagt cgtcgtcgga
     6421 aggaggcact tactcccgta acaaaaacaa gttcgatgaa aacccggaca gttgcaggac
     6481 aaaaacatgc ggtacaaaaa ccactaagaa aagggaatgt accacgagca acaggttcta
     6541 ccacaccgaa atctgttcga cgtccagagg gggttcggga acatatgtca gcagtcaatc
     6601 gagtaacagc acaaaccaaa gcgccttcaa ccgtatctaa agcatactca accatgaaac
     6661 aaacaaaatc accaaaacaa gcaccgaaga cacggagtga gtttcgagca actcaagcaa
     6721 aaatgaaacg aaaacgagaa aaacaaccga tttatcgaag agaaatgaaa caacgaaaac
     6781 gataggagga aatcgatagt atgatgaatg caccaaaacg accgacacat tttgagcaaa
     6841 tgttttctgt cgatcgcaaa agaaaacaag aaaaagaaac gccaaaaagt cggtttcaac
     6901 aactttattt tatcgtcact ttattaggtg tttgtctata tccatttttt attgtaggca
     6961 gtattccttt agcgattgtc tttatgatag ataaaaaaga gacacgtgca catatttatg
     7021 actttgagta tgagagcttc ttaaaacgaa gaagctctct tttttttatt tttgcgttag
     7081 gttttacgct attaaatcta ttttgtttta tttttgttct tcctagaggg tatttgtcgt
     7141 gttacttatt atttccatta aacttgtttt ctcatgcgtt gcagtttaat ggacaaacga
     7201 tttttgccct ccttttaggt gggacaggtg caggaagttg tttgattgcg tatgcgtctt
     7261 ttattgaaaa aagaaaagtt gtttcaaaga aagaacggca aacagctttg ctaaatagta
     7321 aagaatacaa ggaacgtcgg aagaataaat ttcaggagtc ccaaaaatat acagcagagt
     7381 atcagcaagc gtaccaggta gcaattgtca taaaagacgt tgctttgcgg aataaacaac
     7441 tggcttcatt agccaaagtg attctattag gaacagatga gtatgggctc ccttatgtga
     7501 tgaactttag tgaattgaac caacatgtga ttattccagc gactacaggt tcaggaaaaa
     7561 cgacggtgat ccaattacta gcagaacatg cgtgtaagtt tcgtatcccc gttatcttag
     7621 tcgatgggaa aggagcagga gagactttac aagcgttaaa agaagtggca gaggcataca
     7681 atcgcccgat tgaagtcttt gtggacggtg gcgatttgcg ttataatcct gtaaaaaatg
     7741 gcaatgatat tgccattcga gataaattaa ccaatctagc agaaacagaa agcgtgtatt
     7801 attcgacagc agccaaagcg ttattacagg taacgattca gcttttagac gagttccaaa
     7861 agagccataa aattgaaaga aatctagttt ggttacaaca ttatttattg ccacgaaacg
     7921 tcttacattt gtttgcggat cgtattcagt tgaaaaatcc agatttattt aaagaaaaag
     7981 tggtcgtaga aaagaagaaa accacgaaga aaaaaacgaa aaagaatgaa gcgatggaca
     8041 aaacagaacg tgattttgcc gatgtggaac agttaagtga agagcgaatc gttgaacatg
     8101 atcgtaaaac cgttgactta gaaacgtatt atctattatt aaaagaaaac atttctctca
     8161 tgacagaaat cgagcaaacg ttgtttaaac ggttgtttgt tcgttacgaa cataagaaga
     8221 acccgtttta tttgtatgcg acttctgaga atttacagac caatatcaat atgttattgg
     8281 atagtgagct tggtgaactc tttgatactt ctggaggaaa aagagaatta gatattcaag
     8341 aagttgttaa agaaggaaag attgtttata tctcgttaaa cggattgatt tatagtgagt
     8401 ttattcggac attggctcaa atgcttgtgg gagatattaa tttctttgcg tcagaagtgt
     8461 ataaacaagc aggagaaaca gccaatcgct cgaaagtcct agtaatattt gacgaaccag
     8521 cttcttatct aaatgataac cttatagact tagtcaataa gggacgaggc gctggggtgc
     8581 atgcggcgta tgcccctcaa acgcttgcgg acattgatag aattgatcct gtccttcgaa
     8641 aacagttgat tgggaatgtg aatacgttct ttattgggaa gacaaatgac ccgacagaaa
     8701 ttgattattg ggcagattta atggggacag aagaagacat tgaattaacg gaaatgacga
     8761 cacaagaagc aggttattct gatatgggaa aggttgattg gacaggagaa agaggcacga
     8821 aacgagacgt agaccgcttt aaattcaatc cgaatcgcat tcgagcattg agaacaggcg
     8881 aatttgtggt gtatcgaaca gctgaagacg tccaggaacc accgcgagtc gtctatattc
     8941 ggaaaccaaa tttggagaaa caatatgcga aataagaaag ttattgttag gatacttatt
     9001 gttagtttcc tcttatttgg ttgttttaaa ctgataaaaa ctaacgaaga atcaaatgga
     9061 aaactaatca atagtaattt agaagaagta ctagaacttc aaaaaacgaa tcaatcattt
     9121 tttgtgtatc tagcaagaaa tgattgtaaa aaatgccaag aagttgaatc tttcttggaa
     9181 agatacgaca aaaaattctc taggaatgta tatcgaattg aaacaagaaa agaaccaaga
     9241 caagaagaac ttcaaacttt tttacaacgt taccatgtta aaagtgtccc aagttttctc
     9301 gtgattcatg atagaaacgt caaaaaaatt gagagaaaaa cattcttttt tgaaaagaca
     9361 ttgaatcaat ttcttcctaa tttttagtga aaattgaaag agaatacgtt gaaaaaaaca
     9421 aaaaattgtt atgagttcaa atttttttcg atcgtatttg tttttctttt gaaacaaatt
     9481 cactcatttt ttgagcaaaa atgtttcaaa atttgaacaa aaaccaattg aaaattgaag
     9541 caaatttcac tcgaattgaa tcgattttga gctaaaatca atagaaaaaa taaacgtatt
     9601 ttgtttgaaa tccgtttatt ttttttacgt ttcagtatta gtaaggatta tttgagggtt
     9661 atttagagat tatttaaagg ttatatgtaa taagtggggg gagggctgtc ggcgcttggt
     9721 atgaaaggca ttctccgact tccaggtttt tgaccatttt ctctcaacgt ggaaaaaccg
     9781 ccacttatga gagaaaatgg tcaaaaacct aagcgggaag tctccgaata acataccaag
     9841 cgccgacagc caacagataa agaatacact actaaaaata gtagtagcat aaaatttaaa
     9901 agggaaaaaa tcaaccccta ccccagtctt ggggtctcaa aactaactcc cccctaaccc
     9961 cctctttcag cctcttttga aagaggggga gaatcaaccc cacggccaac gccccagtgt
    10021 tcttggggct gtccagaaca gaaacttgcg agagagtggg ggtaaaacaa gtgtagagga
    10081 aacttgaaga cttgttcgaa cagaaacttg cgagagagcc ggagtaaaac aagtgtagag
    10141 gaaacttgaa gacttgttcg aacagaaact tgcgagagag ttggagtaaa acaagtgtag
    10201 aggaaacttg aagacttgtt cgaacacaaa cttacaagag aggggagtaa agcagttgca
    10261 gaggaatcag gaagatttgt tcgaacagaa acttgcaaga aagcattatt aaattgaagg
    10321 tgatgaaata tgaagtattt attgcatggc tattttattg attattctac tcgcaatagt
    10381 agtactttag cgattctatg tttgcttcat gaattgagag tggttactag aaaacagcta
    10441 tacaaactat tgaattttga cagatcaatt agtaaacaag gagtaacgaa tgctttagtg
    10501 agattatatg aaaaacagtt aatcgatcga aaaaaatatg gaacagaaat gtattattat
    10561 ttgacgaaag agggacatca aagcataggt gggtattata cgttgccgaa agttcctgaa
    10621 tataatttga atcaccattt acaaattaac aattatttga ttaaaggaat ggcattggca
    10681 aaagaccacc cacatttaaa acggattatt tcagaaagac gtaccgtcta tgaaacgaag
    10741 gatttaaatc ctaagacaaa aggaagaaaa ttttttgtgc cagattttat ttttcgtttt
    10801 cgagacaaag aaaatcatga gatcaattgg tcctttgaaa ttgagctaac gttgaaaaca
    10861 cgtcgtcgct accgtgaagc gatttttccg aaatatattc gtgagttgcg aacacgtcct
    10921 gaatcacatt taatttatgt gacacctagt ggcgcaattc aagaagaact tcatgcgttt
    10981 aagcgtattt ttttcaagcg cgaaactgaa aaagatgcga gtcaacaaga attgtttgag
    11041 cggttgcata ttttttcagc tttagagttt gaagagggat taaaagcatt attagagaat
    11101 gatccagcag tcaattggcg gacagtagaa cgagaaaaaa tttatccgaa tggcgctgtt
    11161 caaagtcgtt tgaatttatt tgaagaatca actgaaaatg aggaaaaacc aaaagtgaag
    11221 tttacgcctg aagtattgta aaagagggtg ttcgcttcgc tctttttttg tgccagtttg
    11281 ctggcaccgt tcagacgcaa gcgtctgaac cggctcgttt ttaggaagga ctaacttgtt
    11341 tgatttatcc gttgtcaaag aagtggacgt tcgagagtcc ctaatcgtat agcagtcatt
    11401 cggcgagtat aacctaaaac ataacctgcc aattgccaag ggtaaaatgc acgctgcgcg
    11461 cccttggcaa tcgtcagaac atgttttcga gccggttatg ccgaacgacc gctatacgaa
    11521 aagggcctct aaacagaagt ccactttgat ccttgacaac tgaaacccaa aatcaaacaa
    11581 gcaggccttc gtctaaaaac gagccgaatg aagaccttgg gggttgcacc cccaaacccc
    11641 cgtgcccccg acacactacc gaaaaggtgg gggtgggtgg cgaaaaaatc aaataaaaca
    11701 ttctattcaa gaaaggaaga aacaaccatg acaactaaaa acgagcaact gaaagaacag
    11761 gcaagaaaag agttgcaaga aagaggtttg attattgagg ggagctttga aggagatttt
    11821 gaaacctata ttggttgtta tgcaagaccg ataaacaaac ctacagcctt agacccaatg
    11881 aacgaacaag aagtaaaaga acaagaaaaa catgcagtga atggttttcc gcaagatttc
    11941 actgaatggt atgagtggga aattaacaac gggaagcttg aaaacttctt ataaaagtca
    12001 gatgacgtgc aaagagaatg attctctttg tacggtaaaa atataaagaa agaaggaaaa
    12061 agaaaatggc aaaaaacaca ccttatcaag taggagacat tttttatagt agttggggct
    12121 acgaacaaac gaacgtgact ttttggcaaa ttgtgaagtt aacagaaaaa acagcatggt
    12181 ttcagcctat tcaaaagaaa acggtcgagc attattcgtc catgagtgga aaagttgttc
    12241 caatgaaaga caagtttata gagtatcctt tagcaaaaac taaagacagt attgtaaaaa
    12301 aacgaattga tccagaaacc tatcctgata tgttatgtgg ttatcgtagt tgggacacgt
    12361 ttcatagata taaaaatcaa gctgtttatg aaagtagtta ttattaaggg gatagtttcc
    12421 cctttcaaaa ataataaata gaagaatgga gagaaaaaat catgatgaat tcagtgaatt
    12481 tagtaggacg gttaacaaaa gaagtggatt taaaatacac accaaaaggc aatgcgacag
    12541 gcacatttat tttagctgtt aatcgaaatt atacgaatgc aaacggcgag cgtgaagcag
    12601 attatatccg ctgtgtcatt tggcggaaag ctgcggaaac tttagcaaaa tatactggaa
    12661 aaggaagttt gattggcatt aatggacgtc ttcaaacgag gagctatcag aatcagcaag
    12721 gacagaccat gtatgttacg gaagttctag tgacagattt ctatttgtta gagagtaaag
    12781 aaatcaatga gcaacggtca aaatcaatgg cgaataatca aaataattct tcaattccag
    12841 gaaatgagat tcaaatttca gatagttatt tgccctttta agtgaaaaat aattttgttc
    12901 tatacctgat ttcttcaggt atagaacaaa aaataaatag agatgaggcg attttttatg
    12961 aaaatcattg acagttacga tcaacgaacc atgcaatatt tgggctcgtt tgattataca
    13021 gatgaaaata ttattaatta tgttgcaagt ttacctcctt atcaaagcgt tcgcttggta
    13081 gattttgcaa gtgatgaatt ggtattaaca acgatgggca actttttaga ttatgtcgga
    13141 gacaaaaaat ggttggaaaa aattttgtct aaattgattg cgaaacaaac aggagaagaa
    13201 cccataaatg cgattcaatt gttcaatcga tatgaacaag aaaacaatga ttaaagagga
    13261 gagaaaaaat gaaaagatta aaacgaagta tttttattgt gctagcattg attgattttg
    13321 ttgggacagg ttgtgaagca attgacgttc aaaaagctcc cttatcggaa ataaaacaag
    13381 caataacaaa acaagatgaa gaacaaccgc taaatacaca agtagaaata gtgaatcaaa
    13441 ataaaccgag tttttctgaa gaagattttt cgttagaaaa tggaaccagg caattttttt
    13501 cagatttgga tcgtttaaat cgggtaggtg tagcaagtgc acttttgcat aaaagcagga
    13561 tgccaacaga agaacgagac cgattataca ttaagccaac tggctggaaa caaaagaaaa
    13621 tgaagaatgg cgattatctt tataatcgtt gtcatttgat tggctatcaa cgtgcgacaa
    13681 gtttccccat aagtatgtaa tacgaaacgg gaggattaat cttaagatta atcacaacta
    13741 acctatttga cagttggaat gaaagctatc atgtctgctg aatcaactgg tctgatactc
    13801 ctacatgcaa taaagtctac taggaagtta ttgtatgaag ctaggtaaag gcggcagaac
    13861 aatttctaag tgtggaatca tatgaagttg gatatgccgg gacgctataa attactcagg
    13921 gtgagactgt ttttaattaa actgacgaac ttgcgaatgt acgggtctat aaccgactta
    13981 tattcgaaag atataagatt gcgaatgcaa agaacattat cgaaaagtaa gaatgttggt
    14041 catgaaattc gagatggaag actagcggtg tatctgtcaa gagttggtaa aacatgtttg
    14101 attgattcag atgagagtgg gaatgcgaca aaacgtttta cagaagaaat agagcaacta
    14161 gcagaactga atatacgata tttttaaata ctttagcgga gaataaaaaa atcctctaca
    14221 gaaaactacg gagggcaaca tgtgctattg tttcaacaat acatttttaa tttttaccat
    14281 aacaaaaaag tacttgaaaa aaaacagaaa tatgttaaat taaaaatgaa aaaatggaag
    14341 cggttactta ttcttttgaa tctaagataa aaattcaaaa gaataacata acccaaatat
    14401 gattatactt gagattctaa atatttatcg aaaaggaatt tgttttaagg aaaaatgatt
    14461 ctatggacgg ttattgattt atatttacca aatggctaat aaa
//
LOCUS       DACDTO010000072        14478 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDTO010000072 DACDTO010000000
VERSION     DACDTO010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.072"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(287..649)
                     /locus_tag="IWK70_002144"
     CDS             complement(287..649)
                     /locus_tag="IWK70_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614657.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(723..1103)
                     /locus_tag="IWK70_002145"
     CDS             complement(723..1103)
                     /locus_tag="IWK70_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9614658.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1185..2360
                     /locus_tag="IWK70_002146"
     CDS             1185..2360
                     /locus_tag="IWK70_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9614659.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2437..3021)
                     /locus_tag="IWK70_002147"
     CDS             complement(2437..3021)
                     /locus_tag="IWK70_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9614660.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3222..4220
                     /locus_tag="IWK70_002148"
     CDS             3222..4220
                     /locus_tag="IWK70_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9614661.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4332..4841
                     /locus_tag="IWK70_002149"
     CDS             4332..4841
                     /locus_tag="IWK70_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9614662.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4871..5530)
                     /locus_tag="IWK70_002150"
     CDS             complement(4871..5530)
                     /locus_tag="IWK70_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9614663.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5532..6296)
                     /locus_tag="IWK70_002151"
     CDS             complement(5532..6296)
                     /locus_tag="IWK70_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9614664.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6304..6981)
                     /locus_tag="IWK70_002152"
     CDS             complement(6304..6981)
                     /locus_tag="IWK70_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9614665.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7018..8403)
                     /locus_tag="IWK70_002153"
     CDS             complement(7018..8403)
                     /locus_tag="IWK70_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9614666.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8613..8697)
                     /gene="ffs"
                     /locus_tag="IWK70_002154"
     ncRNA           complement(8613..8697)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWK70_002154"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IWK70_002155"
     CDS             complement(8720..9235)
                     /gene="tadA"
                     /locus_tag="IWK70_002155"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9614667.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9342..10199
                     /locus_tag="IWK70_002156"
     CDS             9342..10199
                     /locus_tag="IWK70_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9614668.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10280..10615)
                     /locus_tag="IWK70_002157"
     CDS             complement(10280..10615)
                     /locus_tag="IWK70_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614669.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10682..12052)
                     /locus_tag="IWK70_002158"
     CDS             complement(10682..12052)
                     /locus_tag="IWK70_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9614670.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12130..12666)
                     /locus_tag="IWK70_002159"
     CDS             complement(12130..12666)
                     /locus_tag="IWK70_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9614671.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            12975..14456
                     /locus_tag="IWK70_002160"
     CDS             12975..14456
                     /locus_tag="IWK70_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9614672.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4460 a         2883 c         2353 g         4782 t
ORIGIN      
        1 ttttttaaat tttttagaaa tgtgatataa catccacttc agaatcaacg ctgttaaatt
       61 tatcatgtat ttcttccttg gtcaactata aattaagcat tactttgtga aatgattttt
      121 cgatcaatta ttattattcg ttatacaacc gttttcaaca tattttgaaa ataaaatgca
      181 ctaaaaaact atctttagca gaacatttca ttgtattttt tggaccaaac aataaaaaaa
      241 gaagtttgaa aaaaattcaa acttctttgt tcgcttaatc tgtattttag aagaaaggca
      301 ctccctctac ttcgtaagtt ccaagaacag agcgcatttg acctggttct tcccaaatat
      361 atccttcgcc taattcttct ctttgattct tttgttcagt ttcaaagaca aacaacggaa
      421 cattttcctc tttaatatgt gtatttgtca attccatcaa actaatcttg ctaacatcga
      481 tatgaactat ttcttttaag aaattaagta tattcgctaa accagtcttt ccttcagaaa
      541 gttccgctaa cgcaaattcc aacttgtcat caattgaatg gaccaaaaat tttttagtac
      601 catcctccaa atgcaacata attgtaccag caactctttt gcttgccaaa atttccacct
      661 cgatcaactt gttctatggc ttacataaaa attctagcac attttcagaa aaaatacact
      721 ttttatttct tattttcttt ccgttctagt tcagataatg cttcttctat ccaaataggt
      781 aactgctccg caatagtggt aaatccgatc ttacgatttt tattcgtgaa ataccttttt
      841 ctgcgatatg gaacagctgg atttgttttt tctaacgttc ttaaagataa actgattttt
      901 ttggaatatt catcaatgtc gatgatctga actgttactg gctgtccgac ttttaacaat
      961 gaatggatat ttttcgtata accggattgt acttctgaaa catgaataag tccttgcgtc
     1021 tcgttatcaa gagaaacgaa tgcgccataa ggctgtatcc ctgtcacttt tccttcaatt
     1081 atttgtccaa ttttataagt catctacttc cctctcttcg catttatcat tatactgtaa
     1141 atagctcaaa aaaagaagcg acgaaaatga ctggaggtct taagatgatc gaatttgata
     1201 aacagatttc aagaaaagat acagaaagtg taaaatggga cgcaattgct gagacatatc
     1261 aaatggatga cttacttcct ttgtgggtag cagatatgga cttcttgtct cccggtgggg
     1321 tagctaaagc gtttagtgaa tacatcaaac atggaatctt tggttatgcc actctgtctg
     1381 ataaactata tcaggctatc attcattggg aacgccaaca ccatgctgtg gagttgacga
     1441 aagaaaatat cgtttttaca agtggtgtgt taaccagttt agctgcagca gtacaaactt
     1501 ttacaaaacc aggcgattct attttgattc atgacccagt ttatcctcca ttttcctcta
     1561 ttatagaaac aaatcaacgt cacatcgttc gaagctcttt gctagaaaaa aataatcatt
     1621 tcatcatgga cttgtctgac atggagaaaa aaatcgaaga aaacaatgta aaagcaatga
     1681 ttttatgcaa tcctcacaat cctggagggc gtgtttggag caaagaagaa ttgaaacagt
     1741 taagcgaatt gtgtttaaaa catcaagtga ttctgtttag tgacgagatc catcaagatt
     1801 taactttatt cgatcataca tttacttcca tgctaacgat tgatcctaat ttagatgatc
     1861 tactagttgc ttttacttcc gcaaccaaga ctttcaatct agctgcaatc aaaaattcaa
     1921 tggtatttat caaaaatcca gagatgaaag ctgattttga aaaacattta gtgatgaacc
     1981 aacagcacga aatcaacact tttggactga ttggaaccca agcggcttat gagacgggtg
     2041 gagaatggtt aagccaactg attccttatc tagaaaaaaa tgttcaaaca gttaatcagt
     2101 tttttgaaca gtatcttccg aaagtgagaa taatgaaccc tgaaggaact tacttaatgt
     2161 ggttagattt taccgcctat atttcagacg accaagaatt ggagaacact cttgttcgga
     2221 aaggaaaagt cgtattaaat cctggcatca cgtttggtcc ttccggtcat gggcatatgc
     2281 gcttgaatat tgcttgtcca gaagaaactt tactggaagg acttaatcga atcaagtatg
     2341 ctttagagac aatcgattaa atcatttatt caaaataaaa ggacgagacc ttactaggtt
     2401 tcgtcctttt tcataattac tccgtaccat accaaactat tctgaaattt caattgtttc
     2461 aatcactaca tcatgtacag gtcggtcttg tggaccacgt tgtacatttg cgatttcatc
     2521 tactgtgtcc atcccttcta gtacatgtcc aaagactgta tgtcggaaat ctagccaagg
     2581 agttccgcca cctttatacg cttcaatgat ttctgatggg aatccagcac cttctagttg
     2641 tccaagcata ttagctggca catttgtgtt atttacaata aagaattggc ttccgtttgt
     2701 gtttggacct gaatttgcca tagataaagc tccgcgaagg ttgaacagtt cacgactgaa
     2761 ttcatcttca aaactttcgc cataaatact ttcgccaccc attcctgttc cagttggatc
     2821 gccgccttgg atcatgaaat caggaataac gcggtgaaaa atcacgccat cataataacc
     2881 ttttttggaa agttcaatga aattttgaac cgtcttcggt gctagttcag ggaataattg
     2941 aacagtaatg tcaccacggt ttgttttgat aacagctttt ggtccttttt cactttttaa
     3001 atctagttgt gggaatgcca tattgtttcc tcctaatatt tgcatacttc ataataataa
     3061 taacataata cctaacctct cgtaaaaata ctagatttgt tttcttttat ttttcatcac
     3121 tagatacatt ctataactta ccgtttcgat ttcgaattca aacatttttg agaagcattc
     3181 tcaattaaat tctgctatag tgtgaaaagg gaggaatttt tatggaaatc tatgacatta
     3241 caatcatcgg tgcaggtcct gtagggatgt ttgccgcttt ttacgcagga atgcgccaag
     3301 ctaaaacaaa aatcattgac agtctacctc aacttggtgg tcaattagca actttgtatc
     3361 ccgaaaaata tatttacgat attcctgggt atccagcaat aaaagcaagt gaattaatcg
     3421 atcaattaga aaaacaatta actactttta accatacatt ccatttaaaa gaagaagttc
     3481 tctcgcttac tcgtgaagac gaagttatcg agattactac aaataaaggc atccattatt
     3541 ccaaagcagt cattttagct ttaggaaatg gttcattcca gccaagaaaa ttgaatttag
     3601 ataatgctga atcatttgaa aaccacggat tggattattt cgtgaatgat ttgatgaagt
     3661 atgctggaaa aaaagtcgct attgctggag gtggtgattc tgctatcgat tggacgttga
     3721 tgttagagcc tattgcaagc gaagtttact tgattcatcg tcgcccggaa tttcgtgcgc
     3781 acgaacatag tgtatcacgc ttgaaatctt catctgtcaa cttgcttact ccctatctca
     3841 tcgatggatt atcaggcaat aatggggaac tgacagatat ccggttaaaa aaagtgaaaa
     3901 gtgacgagac gattgatttg atgattgatt ctctgatcgt caattatgga ttctcttcta
     3961 atttagaaca tctttcttct tggggacttg atagtaccag aaatgccatt actgtaaaat
     4021 cagatatgtc tacttctatt ccaggcgttt atgcagctgg tgacatctgt acctatgaag
     4081 gaaaagtgaa actgattgct actgggctcg gtgaagcacc tactgcagtg aacaacgctt
     4141 tacactatat taatccaaag gaacgaactc agcctggtca cagcacaagc ttatacgata
     4201 aaaatcttcg tcctagttga ttttttatat ctctttgcat ttcaagttaa acccgaaagc
     4261 agtttcttgc atttttttca tgttccgtta acatgaaata gagaaagaaa aaataaaggg
     4321 ggagcaataa tatggtcgtc aaaactgaga ctttagaaga aaaggcaaga gaactattga
     4381 aggaacgtgg cgtaactgta gaagatatcg cacaacttgt catgttttta caaaaagact
     4441 atatttctga tttgactttg gcggattgta ctgaaagtgt caacagcgtg ctaacaaagc
     4501 gtgaagtaca taacgccatt atcactggaa tccaattaga tatattagca gaaaagaaaa
     4561 aattgatgag tccgttgcaa catattatcg aagatgacga aggcctttac ggtatcgatg
     4621 agatcctagc cttgtcaatc gtgaatgttt acggtacgat cggctttacg aattatggtt
     4681 atatcgacaa ggtgaaacct ggtattttaa aagaactaaa cagccacgat ggcgttcatg
     4741 tccatacctt cttagatgat atcgtcggag cgatcgccgc tgctgctgcc agtcgattag
     4801 cacacaaaga accagaaaaa agaagtcgcc ttactcaata attcaacaaa aaaagaaaga
     4861 aaacgtctat ttagacgagt tctttctttt ttgtttgttt caagctattt tttcctaaga
     4921 aaagaaaaac agcaaaaaat aattctaata atacaaaaaa taagataaag ctatgcatga
     4981 aaaattcttc tggaagtaca ttgatgattg gatcagtaac gggatcgaat aaccagtcat
     5041 cgttgttgaa aaacgtttca tgaaaaagga taaaaaagcg atcaaatcct atcatcatga
     5101 aaaaaccgaa aacaactggc agtaacatgc cgatttgaaa cggacgaatc aaccgccaaa
     5161 gccgatcatt cttttgcaaa tatttgataa acaaaatact cgggatcaaa gtgatgaaaa
     5221 acacagcata atccactaaa aacaaaattt tcacttcata aaagtgatgg gctccgctag
     5281 cagaaactgg gaaatctggc aagctgagga tggttttgaa gggattattg agataattca
     5341 ttaggtgatc aaaatttttc agtaacgtct cttgatctaa agaagtataa tccaaaattt
     5401 gcaaatgtcc gatatcaaat acgtatagtg gtcgaaagtt gatcgtcaca ccaactgcca
     5461 gactgatcag tgtcagaaat aggctgaaca atccagcata ttccatccac tgccatctcg
     5521 aatttttcat attagaagct ccattcatcc agtgattgtt tcacataggt agctgggata
     5581 ggcaacccag gtacatcttc ttcttttgta aaacctgata aaacgagcaa agtatctata
     5641 ttattgcgta tcccagcttg gatatctgtt tcatagttat ctccaaccat gattacttct
     5701 tctttttgca gccctaatac ttcaacggct ttatccataa tgatggcttc tggttttccg
     5761 atgtagatcg gcggcgtttg ggttgctgtc tctacaaaag agatgactga ccctgctcca
     5821 ggaagaaggc cgcgttcagt aggtatattc ttatctgggt tcgttccaat gaaagtagca
     5881 cctttttgaa tagcaagtgt tgctttaact actttttcat aggtcagata attatctaag
     5941 cctaccacta catagtcagg agcctcttct tcccatgtaa atccggcagc taaaatcaag
     6001 tcaatcaatc ctgcttctcc gataacatag acttttttcc cttttccatc tgctttcata
     6061 aaatcgattg ttgccaaagt agctgtgtag actgtctctg gagctacatg aatgtcaaac
     6121 tcatctgcta gacgattagc tactgtttct ggtgacttag tcgtgttatt ggtaacaaac
     6181 aagaaaggta attttctttt ttgcaattct tccacaaatc gtttaccggc cggtatgggt
     6241 tgtgtcccac gataaatcgt tccgtctaaa tcaattaaat atcctttata gtccattcat
     6301 ccactactct tccctttttc tgatcgtaaa tgctgttttt gttttttcta cttgtttagg
     6361 ttgttcttgt ttttttactt tttcagaggt ttttgctgac tctgtttttc gatttttaat
     6421 aaccggtttt ttgcgattag atgcgactgg tccttttttc tcatcgatat acgcacgatt
     6481 atggtttttt ttcttttttc ggcgctgagt tgtcttttct tttttaccgc cgattctctc
     6541 gatgacaaaa tacgcacaac caaaattgca atattcgtaa agatagtctt ccaaggaatc
     6601 gattcgctga tctggatgcg cccgtcgatt atccgcttgg aaaaatcctt ttaggcgtag
     6661 ttgttcataa ccccaatctc ctacgatata atcatatcgg gctagtacat cactgtaacg
     6721 ttctccaagc ttttctgcat taaatccttc acgataatcg gacacaagcc gataatgtct
     6781 ttcacctatc aaaaaattac tgtcatctaa cagtgtcact agctcgcctt tgactttttc
     6841 tgctttaggc tcctcaacga tttcttccaa agcagctgtc agttcttcgg tgactgttgt
     6901 ttcctgttca gctgatgatt ttttcttagc aatttctgtt ttatgctcaa cgttctgctg
     6961 ttcattttgt tttgcagaca tgcgttcacc acctatcttt tttattatac cttgttttta
     7021 tttgattggg tagtgagctt tcaagtaatc tcctaccaca cttcggataa attcagggaa
     7081 caaaaattca ttttttccag caatctcaat cgttggaaaa aatggaacaa acatcaaatg
     7141 atctacggta gtaaaagtat aatttttatt cagatcgatt ggcttgttct tccaaaagac
     7201 ttgatggtta atagaatcat atgtgattcc attatagacc atctgcccaa aaatcttacc
     7261 gcgaaatccc attcccttca taggatagtt acgtaaaaag ttacgatttt tttctatttc
     7321 taaaactaag cggatcagat catttccacc caatgtgaca tttaacaagt gcatcggatg
     7381 cggcaacgca gtgtgaagct gatcctgatt gacccgccct tttggaattt cagttaaaaa
     7441 cagaccactg tttaatattg ctgcttccgt ctttcctcgt tctttcaccg cctcaaaggc
     7501 tacttggata aacgaatgct cattgaagat atctagactc agagaaaatg gcaaatctgc
     7561 tacttctttt tcttttaata attcatgccc acgttctaga taactattta tttcttgttc
     7621 atcttctgta aaagctgtca ttgtttcggt agggatggct ttggctgaat gcttaaggat
     7681 ttttttctct tcatcgatgg ttaaatggac ttcaccgaca tactgcccgt actttccagc
     7741 ggctgctatt tggacatcat tgattacgtg accttctctg aataaatgat gggtgtgcga
     7801 tcctaaaata acatcaattg ctggtatttc ttttgcaatt tggaaatcat cctgaatccc
     7861 gagatgactc atcaaaacta aaacatccac ttgtgaacgt aaatcttcga ctaattctgg
     7921 caaaatatca taaggattcc gtatgtccca gccattcgga ttataagtaa gcggaaatgg
     7981 agcagtaaat gcaattagtc cgattttcgt ctggtgtttc gttgtaatga ttttatatgg
     8041 tttcgtccat gccggtggct ggagcgtatt tttatcgaat aagttatcca ggatgatatc
     8101 aaactcagca tgatcgtata aatgattcaa ctcttctttg gaatttccta cgccttcatt
     8161 gtttcctatc gtcgctgcat cataaccaac ttcattcatc agcaagatat tttcctgtcc
     8221 attcgttgct tctgacaatg gatgccaacg atccacaaaa tcacctagat ctactgcaat
     8281 cgttgtcgtt ttttctttct tttcgttttc tcgtttacgt tgatccaaaa accttctgat
     8341 ccttggccaa ttttctaaat gagaatgcag atcattcgta tgtaaaatca ctattttttc
     8401 catgtaccct ctcgcctctc tttatcttta tcataccata ggcagctttt tcttccattg
     8461 aaacaagctc tatttcagag tttttcctgc cttgaagcaa attgtataag tacaatattc
     8521 gataaatcag tctggctaaa aaatcaggaa cattcatttt tacagtacta taaaaataaa
     8581 aaaaacagac ccgaaagtct gttgtcgttt cattataatc agacacatgg cggaacctgc
     8641 ttagtgctgc ttccttccag acctgacacg cttcacaagc ccaccattgt cctagccttt
     8701 cggcaaagaa tagtatacct tatttttcat tcgttgacca gtcttttttt aatttctttt
     8761 ttcgctggcg gagctttcgg aaaaagtcag tcaaaatcat cccacattcg tcttctaaaa
     8821 tgccaccttc tacatatgct tggtggttaa accgctcgtc ctccaaaaga ttcatcaagg
     8881 taccggctgt tccgcctttt ggatcatacg cgccgaaata aacttctgct acacgagaca
     8941 aaatcatcgc gccgctgcac ataggacaag gctctaaggt aacaaaaagt gcagcatctt
     9001 ccaagcgcca gcttcccact ttttcacagg cttccttgat tgcaagcatt tctgcatgtg
     9061 tagtagcatc ttgcgtggtt tctctaaggt tatatcctct tccaatcact tttccatcca
     9121 atacaaccac tgctccaatc ggaacttcat gaagcatttc tgctttcttt gcttctgcca
     9181 atgcaaggcg catccatttt tctttttcta ctgcatcaag atttgtattt accaattacc
     9241 cttccctgcc tttgtttttt cttgttttac tttagcatat gataaaaagg ctgtaactaa
     9301 cttttttttc tatggtaaaa taaatacaag aggtgaacaa gatgaaaatg gataaattac
     9361 ttgctcttta cccacaagca cagaaaaaaa actctccagc atcagaagcg gatatccttt
     9421 cttttgcaat tgatgatgct tttatctgga tcaaacaaga ttcattatct caacaagaaa
     9481 ccagcctttt gaaagcgtta ttccctgtaa tcaacgatca aaaaaaacac ccatggtatc
     9541 gctatctttt taaagatgct tcttgtgttc aagaaaaaag ctttcgtttg atccagcttc
     9601 acgtcgaatc aaaaggagaa ttcttaaaaa aagaatggca agatactatt ttggaattat
     9661 ttcgctattc tgctgatttc tttttttata cagacacaga tgcgctctta gtcgaacaaa
     9721 taaaccgtga ttatcttgat gctactgaaa tcaacggtat ttttctatct ttagatgctg
     9781 attttgatac gacaacgtct gtattcgttg gttctttcca ttcacctggc accgacctcg
     9841 tccctttgtt tgctgaagaa agaaaaattt ttttaacaga gcaaaacaat ctttatactc
     9901 acagccgtac tttttctatg caagatgtgg ctttacatta ctatacaaaa gatactgtaa
     9961 aagatagtaa gttgatcaaa agttacatag aactgatcaa acaaaatgaa atggaagtga
    10021 tcattcaaga attatggaat gaattaggca atgtcagctc tgctgcaaaa acactgtttc
    10081 ttcatcgtaa tactctaaaa tatcgaatcg aaaaatttca agaacagtct ggtttcaatc
    10141 taaaaaaagc caacgattta ctgttttgct atcttttatt gcttcatgaa cataattaaa
    10201 gatagattaa aggggttgtg acaataattt tgtcacaacc ccttatttcg aatgaatagt
    10261 gttcaaaagc tagtttctct tacatcattt tttcaacaaa atgcattagc cggtctttta
    10321 catcttcggt tgaatctttg attgaatcac cataaacaga gattttcttt tttcctgatt
    10381 tgatttttgc taacattccc atcaaagaat ccaagtcact atctgaaaga ccatctacga
    10441 tttctagtaa tttttcattt ccatcaaatt tgtcatagac gaattttttt actttcgtcc
    10501 gagtagtcat atgatggatc ttttccatta cttttcccga tacaacgact gctgtcgcta
    10561 cactggcagc ggctgctacg cttaatccga ttgttacttt tgttgatgtt ttcataagta
    10621 tcagctcctt tttatctatc tccatcatag catagacaaa aaaataaacg aactaatcca
    10681 ctcagtgaaa tctcaatcct ttagggaaaa agttctttac tgttcctttt acttgtttcc
    10741 caaatccgac aggtatctta tctaatacta gcaaaaccca tccttgattc ccatcacgtt
    10801 gaaatgtttc tcctgctgtg taagattgcc attccttttg agtgatcggc aagcaaggta
    10861 tattctctat cttttttgta gcaagcgcta atgcatagct aggttcaaat cgattctttt
    10921 tgaaatctcc taaatgcaaa cctgtacgta cgacttttaa accatcgaga gaaggggcta
    10981 actcaggaac ttcccaaaga tggtcgttga acactaaaag tcttccagta gcttcataat
    11041 gaaaatcatt tgaaaactcc gtccagagtt tttcctgctc ttttgtcatt tgcactttac
    11101 ttttttttcg tgtcttcttt tctttatgca tctgattttg tccatgaaaa gtcagctttg
    11161 caacaaaatg tccttcaccc tgatctttat gaggccatat acgtattgtt ttttccaaac
    11221 cagctacgct tccccattca gaacgtcctg aactaacgga ttgagtcaaa ggaatctcct
    11281 caatcgttac tggataattt tctacaagcc agctaatgat ctcttcattt tcttctggtg
    11341 caaacgtaca agtcgaataa atcagttgtc ctttattttt tagcattttt attgctgaag
    11401 acaaaatctc ttgctgacgt ttttgacagt atagcggaga ttcttctgtc cactctttta
    11461 ttgcatttgg atcttttcgg aacattcctt ctcctgaaca aggagcatca accacgatcc
    11521 gatcaaaaaa gcccgaaaaa tgagggacaa gttctgcggg tgcatgattt gtcactattg
    11581 cgttcgaaac tccccatcgt tctatatttt ctgataaaat cttcgcacgt ttcggaaaga
    11641 tctcattcgt cactaataac cctttcccct tcatttgagc agctaattgg gttgatttac
    11701 caccgggagc cgcacacaaa tccaaaactt tttcaccggg ttttgctgca gcagctgttc
    11761 caacgatcat tgcacttggt tcttggctat attcataccc agcttgatgg aggaaagact
    11821 ttccattgac agtgccaaga aatccctcat tgctgtaggg agcgggttgt aattcttcgc
    11881 tatggtaagt ttggaccatg tccaatcctg ccggttttag tggattccac cgaaatcctt
    11941 ttttcacgct tccttgttca agcgcagaaa aaaaatcgga tgcttcttcg cctaaaagaa
    12001 gacgatattt tttgataaat tgctgtggta acgttgtcgc ttcctccttc attccaatgt
    12061 ttcgttagac attttacaat cttttttacg aaaaataaag gtggctgatc tactttttca
    12121 tgttgctgcc taaggtgact gttcctttaa gatattttcc aagtattcat ccgtcacctc
    12181 gtttttatcc caaacagcgt tcatcatttc aagaaaatct tgagaagaat catctgagcg
    12241 caaacttccc aatgcattaa gccataccaa ttcaagcgac ttttcttctt tgatcttcgt
    12301 gtgacctttg acatatgaga aaaatggccg cttaaatagc gaatccatgt ctaaatcaga
    12361 ttttgcaatg atttcacgaa tactttcatg gatagaaaaa aaatctttca ataagtagac
    12421 aatgcttaaa acatcatcgg aactgcattc agctaagtag ttttcaaaat aaacagtgat
    12481 ttcttctaat atcttctctt ttaacccgct gattgatttg aaatgtgtat aaatcgggta
    12541 tgtagaacaa ccgcagaact ctgctaactc tcttgcagta attgacttca ttccatattc
    12601 ttccacgtat accatacttt tgaacagcaa atattcttta ctataatttg ttactctccc
    12661 catcattctt acctccgtct cttttcatct acccataaat aaataacata aataattcta
    12721 aaaatattat tgataattat caaaacatat ttagttatat ccattaaaaa tcaatttttc
    12781 cgccttaaaa acaagaatat cttgacaaaa taccctagga aataaacaat agaataaaca
    12841 aggtaacaaa aaaattaaat aacgttatat aaaaaagaag gcgacactct gtgagaataa
    12901 cacagactga aatgactttc cactcacagt ttttgtctaa ctatatacaa aaacagaata
    12961 aaaaggaggt atttatgcta attgaagcaa gaaaaaaaga gttacttgtt ctacaaatgg
    13021 tcattcaaaa aaataattgt tctttacaag aattatccaa tgacttacat attccaaaac
    13081 ggacaatcaa agaactgatc cgaaaaataa atgtatcact caagcaattt ttattgattg
    13141 atgaatttat ttattctaat cataaaggag agatccaaac caaagaaacc gatcaaacta
    13201 aaaagttagc tgttttttct aagttaaaat tatgctacct aaaagaatcc aatcgtttta
    13261 actatttgat gttactaatc aattatccac aaactgctgt accaaaaaaa tatttacttg
    13321 aacaacttta tatatcgccc tcctatctcg aaaaactgac aaaacagctc aatcattcct
    13381 taaaaaaatt ccatttacgg atcctatctg taaaaaattg ctgtactctt gaaggaaatg
    13441 agttatctat ccgtctctat ctttactttt ttctttcaga tacatttgga ggtaacgatt
    13501 ggccattttc ctcacttgaa ttaaatgcac taaaaatgaa cagtacagat gagcaaatag
    13561 aaaaaatttc aaaacaagaa aaaaatatct acttgctgtt aactttattc atattacgaa
    13621 acaaacaaca ggcatatttt ccaaaaactt cttctgaaat cactgaattg atggacttat
    13681 tgcagcatac acatgattat tcagaatact ttacttcttt ttcagtgagt cgcccttgtg
    13741 aagagacaga aacagagaga gcctacttta attatttttt ctattgtttt tttccaaatc
    13801 aaatctcttt tcgacaaaaa caggagattg gttttttatt ttccacaagc aaaaacctta
    13861 tttgtcgaca agtcggtcaa ttgatgactt acttaactca acagtttcct ctcaaccatt
    13921 tagaaaataa aataccaatt tatttttatt ttcttgtgtt ggcagttgtt tttttcgaac
    13981 tgttgggtga atccagcagt agtttttcta cactttgctt tccagaagcc acgtccaatc
    14041 attctagttc ttttatacag gaaaagaaat taagagaaaa aatcgataca attttttcaa
    14101 aaaatgggga aagaaaccaa atcagtcaat accttagtca attgatccaa cacttactac
    14161 aaacagaaac gaaagagcag ttactcatcc atttgcagat aaccaaacag tttacaggaa
    14221 attatcatct tcaaagccat ttgcagcagt tatttcgcaa agatgctgtt aaaattaccg
    14281 catattcaga acatgctgat ttgatcatta cagatcagca tgagaaaatt tcagatgaaa
    14341 cggagacttt ctatctggat acaacgcaca atacagtgtt atttggcaag ttaataaaag
    14401 cggttcatga actgtatatg aaaaaaggtt atgagaacaa aacaaagagg atgtgacaaa
    14461 attcttgtca catcctct
//
LOCUS       DACDTO010000073        14161 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDTO010000073 DACDTO010000000
VERSION     DACDTO010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.073"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            66..767
                     /locus_tag="IWK70_002161"
     CDS             66..767
                     /locus_tag="IWK70_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9614673.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            764..1885
                     /locus_tag="IWK70_002162"
     CDS             764..1885
                     /locus_tag="IWK70_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9614674.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            1900..3132
                     /gene="pepT"
                     /locus_tag="IWK70_002163"
     CDS             1900..3132
                     /gene="pepT"
                     /locus_tag="IWK70_002163"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9614675.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            3264..4172
                     /locus_tag="IWK70_002164"
     CDS             3264..4172
                     /locus_tag="IWK70_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9614676.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            4207..4479
                     /locus_tag="IWK70_002165"
     CDS             4207..4479
                     /locus_tag="IWK70_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9614677.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            4490..5536
                     /locus_tag="IWK70_002166"
     CDS             4490..5536
                     /locus_tag="IWK70_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9614678.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            5787..8396
                     /gene="clpB"
                     /locus_tag="IWK70_002167"
     CDS             5787..8396
                     /gene="clpB"
                     /locus_tag="IWK70_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9614679.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(8547..9218)
                     /locus_tag="IWK70_002168"
     CDS             complement(8547..9218)
                     /locus_tag="IWK70_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614680.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(9211..9705)
                     /locus_tag="IWK70_002169"
     CDS             complement(9211..9705)
                     /locus_tag="IWK70_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9614681.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(9725..10945)
                     /locus_tag="IWK70_002170"
     CDS             complement(9725..10945)
                     /locus_tag="IWK70_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9614682.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            11092..12927
                     /gene="lepA"
                     /locus_tag="IWK70_002171"
     CDS             11092..12927
                     /gene="lepA"
                     /locus_tag="IWK70_002171"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9614683.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(13021..14148)
                     /locus_tag="IWK70_002172"
     CDS             complement(13021..14148)
                     /locus_tag="IWK70_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9614684.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         4575 a         2602 c         2927 g         4057 t
ORIGIN      
        1 ttttttattc ctagtctcct tattttattt ggaaaatgct ataattatct agaaggagtg
       61 aagctatgaa ccatttagat ttatctcagc gcttagccgc agtgggtgac tttgtccctg
      121 aatccgctcg tttagcagac attggatcag atcacgctta cttacccgtt gcattgatat
      181 taagaggaaa aatcgaatat gcagttgctg gtgaagtcgt tcaaggtcct tatgaatcag
      241 cacgtaagca agtcaaaaaa gatggattag aagaccgtat caccgtacgc cttgcaaatg
      301 gtctagatgc tatccagcca caagatggta ttacagctgt cacgattgcg ggtatgggag
      361 gttcactgat ccgagatatc ctagaagctg gcaagttgaa tcaacgctta agcggacaag
      421 aacgtttgat ccttcagcca aacatcggag agcgtacatt acgatcttgg ctgcaagaaa
      481 atcagtataa aatccaatca gaaaaaatac ttgaagaaaa taaaaaaata tatgaaatca
      541 ttgtagccga aaaagaacaa aaaccagtta cttacaatga aatggagctg atgttcgggc
      601 catttctgct tgaagagaaa agtgagatct tcaaatcaaa atggacaaga gagcttcaac
      661 aaagaaaagc tatccgtgaa cagctcaaac aagcagctaa tcaagaggca cgtcaaaaac
      721 aaatcgagca ggaaatccaa atgattgagg aggttttaaa gccatgacaa tcagcggacg
      781 cactttcatc caaaagtttg aaagctattg tcctcagtgg ctagctgaag aaggagaccc
      841 agtcggactg catatcggaa cattggataa gccaatccaa cgagtaatga tgacattaga
      901 cgttcgtcca gaagtggtcg aagaagcaat caagaaaaaa atcgatttgc tgattgctaa
      961 acatcctcca atctttcgtc ctgtaaaacg gctggttact gatcagccac aagaaaaaat
     1021 gtatgcagat ttgttgaaac atgatattgc cgtctatgca gcacacacga acatggatat
     1081 tatttgggat ggactgaatg actggttttg tgaacttctc ggtattgaag tggagagtta
     1141 tttagtaaag actcatgaga ttcattataa aaaactggcc gtttatgtac ctgtggatca
     1201 tgcacagaaa atgcgtgaag tattagctgc agctggtgct ggtacacaag gtgattatac
     1261 cggtacaagt tttacctcga ttggacacgg tcgatttact ccggaagctg gtgcacaacc
     1321 agcgattggg aaagtaggga agacagaaca agttcaagag gcaaaagttg aggttatttt
     1381 gccggaaact atcgaaaaac aagtgattca agccatgcgg tcagctcatc cttatgagga
     1441 acctgcttat gatcttttcg ctatagatga accagtcgaa atgtttgggt taggacgagt
     1501 aggtgaattg ccgcaagaaa tttctataga ggcatttgta gaacaagtaa aagaagcgtt
     1561 ccaactagat ggactgagaa tagtccagcc aaaaaatgca aaatcctctg tgaagcgcat
     1621 tgcgatttgc ggtggaagcg gtgaaaaatt ttatccgcaa gcaatcgccc aaagagcaga
     1681 tgtctatatc acgggagata tctattatca tacggctcat gatatgcaaa gcgctgggtt
     1741 gattgcaatt gatcccggtc attatattga atcgttatgc aagcaacgat ttatcgagaa
     1801 atttgaatct tggaaacaag aagaaaattg ggatattaac tttttcgttt ctgaaacaaa
     1861 tacaaaccca tttcaattta attaaaagga gagaatacta tgtacgaaaa tcttttgcca
     1921 cgttttttac gatatgtcaa aacagaaaca cgctccgatg caacaagcaa aacgactcca
     1981 tcgactcaga cacaagttgc ttttgcgcaa gaattgaaaa aagaattaga agagttaggc
     2041 ttagctgatg ttttctataa tgaagataac ggattcgtta tgggaacatt accaagcaat
     2101 gttgatcatg aagtgcgttc cattggattt atttcccaca tggatacagc agattttaat
     2161 gcagtaggag tcaatccaca aatcattgaa aactatgatg gcgagtctac gatcgtacta
     2221 gatgcggaag aacgctatac attgaataca aaagattttc caaatttgaa aaattatgaa
     2281 ggccacacat tgatcacaac agatggcagt acattactgg gagcagatga caaatcaggg
     2341 attgcggaaa tcatgactgc aatggagatc ttgatcaata atccttctat ccctcacggt
     2401 accatcaaag tggcttttgg tcctgatgaa gaaatcggtg tcggagcgga taaattcgat
     2461 gttgagcaat tcgatgtaga ttttgcttat acagtcgatg gtggtccttt aggtgagttg
     2521 cagtatgaaa cgtttagtgc tgctcaagca aatattacga tccaagggaa aaatgtacat
     2581 ccagggacgg caaaagatac aatgatcaat gctctacagt tagcaatcga ttttcataat
     2641 cagcttccag ccgatgaagt tccagaaaaa acggaaggat acgaaggttt cttccatttg
     2701 atggcattgg atgggaatcc tgaagaagct tctatgagtt atatcattcg cgatcatgat
     2761 cgtgaaaaat tcgaagcacg caaagcacaa atcacacata tccaagaaga gttgaatcag
     2821 cgatttgatc aagaacgaat caaagtagat atgtatgatc aatattacaa catgaaagaa
     2881 atcattgaaa aagatatgag catcgtggaa ttagcgaaac aagcaatgat cgagcttggt
     2941 atctctccag tgatcgaacc agttcgtgga ggaacagatg gttcaaaaat ctcttacatg
     3001 ggtattccga cgccaaatct atttgccgga ggagaaaata tgcacggacg attcgaattt
     3061 gtttctttac aagtaatgga aaaagcaaca gacgtgattg taaaaattgc agaattgaac
     3121 acaaagagtt aaaatcgaaa aagaacagct tatgacaagg cgttcgtccc aatgatggaa
     3181 tgtcatctgt ctaaggctaa agggcaggac aatggaattg gttgtcccgc cctctttttt
     3241 gaagagtaaa taggaggtaa agaatggaaa agctgctagt tttttataac aaaagttcag
     3301 ggaaagatga gggtgagaaa ttagcacgct ggtttcaaga ttatgtaaaa gaaaagcagc
     3361 cagaattata tgtagaacta gctgaaactg gcccatcgat tgatgatgcg gaattagtca
     3421 agaaagcaga agccatagaa gctgatactt tagtaattat aggtggagac ggcacgattc
     3481 atcatatcgt ccaagcgttc caaaaaacat tatctcaata ccatgtcgga ttacttccag
     3541 gtggaactgt caacaacctt gcacgtgttc ttgagattcc cctcaaaaaa gaagaagctg
     3601 ctgacgtgat cctggaaggc aatgtacgga aaatagacta tggtcaagtc aatgatgaag
     3661 tcattatctc tacactgaca atcggaatct tagcagatac agcagtttgg atcagtcaag
     3721 aagaaaaaca gcgttacggc tcgtggatct ttatcaagaa gttttttaag ttattgctga
     3781 agaagaaaaa atatcatttg gatttagaaa cagacgagga gcattggaaa ggaaaatccc
     3841 aactgttgac agtcacgatg tctaactccg tgggcggttt tacaaatttt gacgactcag
     3901 cgaccctaga tgatggaaaa tttcatgtaa cgatcgtacc ttccttagat attttccgat
     3961 ttatttttta tttgcctaga atcatgagag gcaaattcta tacgatcccc ggcattactt
     4021 atttcacagc caaacgcatg aaaatccaag cgcaggaaaa atctgtgggc actcgaacgg
     4081 atggagacac gacagatgat cttccagttg aattatctgt tgtatcaaaa ggattaacag
     4141 tttattcacc aaagaaagaa gaaaaagctt agaaatagta gttaacagaa agttggaaga
     4201 agagtaatgg atgtattgtt acacaatctt ttgtggtttt accttttcat tgtgaatttt
     4261 tacttatttt gtctgatggg gtatgataaa catcaggcta gaactagcgg ctggcgtgtt
     4321 cctgagaata atttgatatt tgtcagcctg ttaggcggtg gtttgggagg attgatctct
     4381 cgaaaagttt tccatcataa aactcggaaa aagaagttta ctataggatt cgctgcaggt
     4441 gtacttgttg atttgattct catttatttt ttccattaag gagcaggaga tgtcgtctaa
     4501 aggaacgaaa actaaaatca ttctgaatct gcttttgctg attgtgattt ttgccattat
     4561 ttattattta atcaatcaat catttgcaga tatctttgcc gaattgctgt ctaccagtgt
     4621 tgttgttttg gcagcggctg ttatattagg gacagtttac caaatcgctg aagggcgctc
     4681 aatcaaagaa attgctgaac ctttcgcgcc gaattttaca acgaaagatg ggttcttgac
     4741 ctctagctat attgcgtttt accgaatcgt ttcgtttggt acaggaacgc tgatctcgga
     4801 gatttatttt tataacaaaa aaggaatgaa gctatcccag ggagcagggg tgacagcact
     4861 ccatatgatt atgtacaaac tggcagtgat cacttatgca ttagctggct tggtcatcca
     4921 attctctctt ttttattcta atggtccatc aatgatctgg tttatcatcg ctggtatcct
     4981 tttgacgatc gtgattgttg cctttttatt ggcgatgtcc gtcagcttga atgttcaaat
     5041 attttttgtc agcttatcga ataagttatt taaaagtgag cgtttgcgga aatgggtgga
     5101 tacatgcaat cagcagatct actctttaag ggaaacggtc caaaccatta tccaagacag
     5161 gactgcgcta ttgcggattt atctatggaa tatgttaaaa cttgtatttt ggtatgtact
     5221 tccttatttg attctgattg aaaaccatcc taaaatagat tttctactga ccatttcgtt
     5281 tatcagcttt gcagtagtgc tttcaggggt tattcctacg cctgctggta ttggttcctt
     5341 tgaatttgtc tatttgttat tattcagacc agttgttgga acagttgacg cagtttcttc
     5401 gcttttactg tatcgtttca gttcctttat cttgccattt ctctatggat ttgtgtatgt
     5461 gattgctgaa cggcgaagag tgatcaaaga agaaataata gaagtaaaaa aagagaataa
     5521 agaaaatcaa gggtaggtct tttataagat ctaccctttt tatcaaatca aagaaaaacg
     5581 ttatggataa gataatttta cgtttgtttg gctctttttt tatatacata caatcaattt
     5641 tgttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt gtatattttg
     5701 accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat aagtgatctt
     5761 acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg acgcttcaag
     5821 aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat atcgacattg
     5881 cccatgtatg gaaaatcttt ttacaaccta atcattttgg tcgaaatttc tatacggatg
     5941 ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat gagtatccag
     6001 ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc tttcgattat
     6061 tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca acggaagtcg
     6121 ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg aataaaaacg
     6181 gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga gaaagagtga
     6241 cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt gatttagtac
     6301 agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa atccgtgatg
     6361 tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga gaacccggtg
     6421 tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa gatgtgcctg
     6481 aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc gctggagcaa
     6541 aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc aaaaaaagtg
     6601 atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca ggaaaaacag
     6661 aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt gaattacatt
     6721 tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac aaagcgttgg
     6781 aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg atcagtattc
     6841 tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat gacaacgcat
     6901 tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta ccggacaaag
     6961 caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac tctatgccaa
     7021 cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa gctgcactga
     7081 aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa gaattagctg
     7141 aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa gaagaagtca
     7201 attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta gaagatgcag
     7261 aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata cctcaattag
     7321 aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag atggttcaag
     7381 agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt atccccgtaa
     7441 cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca cttcataaac
     7501 gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt cgttcacggg
     7561 ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga ccaactggtg
     7621 tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca gaagatcata
     7681 tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt ctagtcggag
     7741 ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct gtcagacgaa
     7801 atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat gtcttcaata
     7861 tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga gtagtagatt
     7921 tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg ctggaaggcg
     7981 tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg ctattacgtg
     8041 gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt acaccattga
     8101 gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca caacgtctgg
     8161 aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt gctgaaaatg
     8221 cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag gaagtagaaa
     8281 caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa gtaacgatta
     8341 ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc gtctaatctt
     8401 ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac ctcctttttt
     8461 caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt tcttgaagct
     8521 gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa tttataactt
     8581 tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat taaggcagca
     8641 cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg tccgacaaac
     8701 agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg gtcgcataag
     8761 aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc gaataagagg
     8821 aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc aatcaatcca
     8881 tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc ccaaaaagag
     8941 atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa gaaaaaccag
     9001 taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat gacttgtggc
     9061 atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc ttgagaagtc
     9121 gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac accaaaagca
     9181 agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc catctaaaaa
     9241 tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt tcaatttgat
     9301 caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt cttgtagtgc
     9361 agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata agccgatcac
     9421 tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat cgatcgtcag
     9481 cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt cagcaagagc
     9541 aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt cttgaggaag
     9601 atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt ggttatataa
     9661 caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa catcatcctt
     9721 tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc tctagtgact
     9781 tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa gcacggcgat
     9841 aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt cctataggtt
     9901 catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca ccattattcc
     9961 aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca acaaacggaa
    10021 accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt tcgatctgct
    10081 ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa atatctccat
    10141 gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca tttcgagctt
    10201 cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc caatagaaca
    10261 gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc tcatcttcat
    10321 tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca ccatggtttt
    10381 gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt aatcgctcaa
    10441 ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta aaggaaagtt
    10501 tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa ggatacaaat
    10561 aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt agatcttgca
    10621 tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca cccattttat
    10681 aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag atcagtttat
    10741 tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg gaacggataa
    10801 aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact tggaacacat
    10861 aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt aattcaagat
    10921 tttctctaaa atctttttga tccatttttt catctccatt tattagcttg ataacagtat
    10981 agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt taaattactg
    11041 cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc tatggatata
    11101 aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc tcatatcgac
    11161 catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt gacgtctcgc
    11221 gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg aatcacgatt
    11281 aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata tatctttcac
    11341 ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag ccttgctgcc
    11401 tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca aacattggcc
    11461 aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa taaaatcgac
    11521 ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat cggaatcgat
    11581 gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga tattctcgag
    11641 cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct aaaagctttg
    11701 atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg gatcacggac
    11761 ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac atttgatgtt
    11821 acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat ggtcggtgat
    11881 gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg ggatacagta
    11941 acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat gaatccgatg
    12001 gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg ggaagcttta
    12061 gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc tcaagcatta
    12121 ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt ccaagaacgc
    12181 ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat ttaccatgtt
    12241 aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc tgaacctgta
    12301 acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc aaatgatttt
    12361 gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac gatggattat
    12421 ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat tgtctttgac
    12481 tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta tgaaatgtct
    12541 ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga aaaagtcgat
    12601 gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc aatcgttgaa
    12661 aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc agctattgga
    12721 caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt ggctaaatgt
    12781 tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga agggaagaag
    12841 cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc cgttctgaaa
    12901 atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg taaaaaataa
    12961 accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg gtggcaaaaa
    13021 tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca tcttatctgt
    13081 tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc gttcagagat
    13141 ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc tgagagaatg
    13201 gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt tcactacact
    13261 tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa tgtagagctt
    13321 tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa ttgctagcaa
    13381 ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac catataccca
    13441 tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg aaaaatcgac
    13501 atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa atccaatatt
    13561 caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat caaagtcctc
    13621 aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt ttggtatttt
    13681 tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca tattcagcgc
    13741 attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct ttttaaattc
    13801 agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt tctttcctaa
    13861 ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat tgtattttag
    13921 atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc gagtatccgt
    13981 acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta aagcagcttc
    14041 ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc catcatcatt
    14101 gatatcttcg ccgtttgtat aattaagcga gacaaataaa aaagccattt atgttacact
    14161 c
//
LOCUS       DACDTO010000074        14038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDTO010000074 DACDTO010000000
VERSION     DACDTO010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.074"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(30..830)
                     /locus_tag="IWK70_002173"
     CDS             complement(30..830)
                     /locus_tag="IWK70_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9614685.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(827..1639)
                     /locus_tag="IWK70_002174"
     CDS             complement(827..1639)
                     /locus_tag="IWK70_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9614686.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(1834..2391)
                     /gene="frr"
                     /locus_tag="IWK70_002175"
     CDS             complement(1834..2391)
                     /gene="frr"
                     /locus_tag="IWK70_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9614687.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(2394..3116)
                     /locus_tag="IWK70_002176"
     CDS             complement(2394..3116)
                     /locus_tag="IWK70_002176"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9614688.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(3252..4133)
                     /locus_tag="IWK70_002177"
     CDS             complement(3252..4133)
                     /locus_tag="IWK70_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9614689.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(4232..5014)
                     /gene="rpsB"
                     /locus_tag="IWK70_002178"
     CDS             complement(4232..5014)
                     /gene="rpsB"
                     /locus_tag="IWK70_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9614690.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            5373..5852
                     /locus_tag="IWK70_002179"
     CDS             5373..5852
                     /locus_tag="IWK70_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9614691.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            6069..7160
                     /gene="phnW"
                     /locus_tag="IWK70_002180"
     CDS             6069..7160
                     /gene="phnW"
                     /locus_tag="IWK70_002180"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9614692.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            7153..7281
                     /locus_tag="IWK70_002181"
     CDS             7153..7281
                     /locus_tag="IWK70_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9614693.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            7285..7830
                     /locus_tag="IWK70_002182"
     CDS             7285..7830
                     /locus_tag="IWK70_002182"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9614694.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(8284..9975)
                     /locus_tag="IWK70_002183"
     CDS             complement(8284..9975)
                     /locus_tag="IWK70_002183"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9614695.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(10395..11342)
                     /gene="arcC"
                     /locus_tag="IWK70_002184"
     CDS             complement(10395..11342)
                     /gene="arcC"
                     /locus_tag="IWK70_002184"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9614696.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(11457..12476)
                     /gene="argF"
                     /locus_tag="IWK70_002185"
     CDS             complement(11457..12476)
                     /gene="argF"
                     /locus_tag="IWK70_002185"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9614697.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(12567..13796)
                     /gene="arcA"
                     /locus_tag="IWK70_002186"
     CDS             complement(12567..13796)
                     /gene="arcA"
                     /locus_tag="IWK70_002186"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9614698.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3923 a         2927 c         2479 g         4709 t
ORIGIN      
        1 aaattcacaa agaaacgcag gtatacttac taaataatgc caaataaatg catgatagga
       61 aatacaaaca gcaaactgtc gaaacggtct aagattccac catggcctgg taagatattt
      121 cccgagtctt tcacatcata atgacgtttg attgcagatt cgaccaaatc accaaattgt
      181 ccgactgctg aaaaaataat cgtcatcaga agcatgacaa acataccatg attaaatagt
      241 tcttttgctg ggtatagcaa taaataaaga aatgcgacta caacagcact accaatacca
      301 ccgagcgcac cttcaatcgt tttgttgggc gaaatatcag gacttaactt atgctgacca
      361 taacgtcgac caaccatata tgctccgata tcagttgccc aaacaataaa tagaccaaat
      421 atcaaaacag ccaagctttc tgttcgtgca ttgacaaaat tctggaaacc tattcccaca
      481 tacaagctga caagcacagg aaaacctgct tcatcaatcg tatacgtatt tttagaaata
      541 actgatgttc ctaaaagaat cataactata agataaaata acataaatgt acttacattc
      601 tctggaagaa agaaaaacca tctttctttt ggcaaaacaa gtaacactgc accaacggcg
      661 gataaaactc cttcaaaact taagagtgtc aatcctttca tccgaaacag ctcgtaaact
      721 cctattactg ctaagattgc tgctgtaagc tcaaccgcta tgcctccgat ccatataatc
      781 ggaataaaca agactagcgc cacggctgct gtgaccacac gttgtttcat gactgatccc
      841 ctttctcatc ttctgttttt tttacaccgc cgaatcgacg gtctcgattt tgataagagg
      901 cgattgcttc ttcaagatgc gcaccatcaa aatcaggcca aagcgctttt gtaaaataca
      961 gctcactata tgcgatctgc cataacagaa aattactaat tcgctcttct ccacttgtac
     1021 ggatcatcag ctcaggatct tgtaactctt taggcaagaa acctgtcatt aaatgcttag
     1081 caatcaattc atcatcgatt tcttctgcgt gtatttctcc ttttgctact tcttcggcaa
     1141 tttcttggac agcagtcacg atctctgctc tgcttccata attcaaagca aaattcaata
     1201 ccattccagt attgtttttt gtttgctcaa ttgcacgtct tacagcatct tgcgtatgtt
     1261 ctggcaatac gttttcataa cccatgacat gaactttgac attttcttta atcaattctg
     1321 gtacgaacgt atcaaagaag tcgacaggta gttgcatgag aaaacttact tcatcttttg
     1381 gtcgcttcca gttttcagtc gaaaaggcat aaagcgtcaa cactttcact ccaagccttg
     1441 atgctttttt tgtgactttc tttactgttt ccatgccttc tttatggcca gcaactcgtg
     1501 gcaaacgacg gttttgcgcc caacgaccat tgccgtccat gatgatggca atgtgctgag
     1561 gaattggtcc atcggtatta aattgaaatt cgcttttttc ttgaatatat ttgttttttt
     1621 gcggaaaaaa acgtaacatt ctttttcctc ctatacttaa aagattccca ctatcttaag
     1681 tattatagca atatctaact gtttttccta tgaaatcatt gtttttttat ttaaaaactg
     1741 aataattttt tcttttttta atttttaaat aaaagaaata taaataaaaa cagaggaagc
     1801 ggaatgatcc gcccctctgt ttttatgatt ttattaaact tccaataatt ctttttcttt
     1861 atcagcagcg atcttatcta cttctttgat gctgttatct gtcaatgttt gtacgtcttt
     1921 ttctaatccg cgcaaatcat cttcggtaat atcaccattt ttttgttgtt ttttatattc
     1981 atcgatcgca tcacgacgga tgttacgaat cgcgattttt gcattttctg cttctttttt
     2041 aacatcttta gctaattctt ttctgcgttc ttctgtcagt tgcgggataa ctaagcgaat
     2101 gacatttcca tcattggttg gactgattcc gatatcactt gccataatag ctttttcaac
     2161 atcttgaaga atgctcttgt caaaaggtgt gatcattaaa acacgtgctt ctggaatttg
     2221 gattgatgcc atttggttca atggtgttgg cgcaccgtag tagttcacag taatacggtc
     2281 caaaaggcta gcgttcgcac ggcctgcgcg gatttgccca agttcacgtt gaagattttg
     2341 tgctgctttt ttcatttttt cttttgcttc tgtcataatt gcatctgcca tttttatttc
     2401 ccccttacag ttgttccgat attttcgcct aagattgcac ggcgaatatt tccgtgttca
     2461 tttaaattga agacaagcag tggaatatca ttatccatgc ttagtgaact tgcagttgaa
     2521 tccatgactt gtaatccttt ggcaataact tccagatgag tcagttcttc aaattttact
     2581 gcatttgcat ctactttcgg atcagcagaa taaacaccgt ctacattgtt tttagccatc
     2641 aggataacat ctgcgccgat ttctgccgcg cgtaatgctg ctgttgtgtc tgtcgagaag
     2701 tacgggttgc cggtaccgcc tgcaaagata acgatccgtc ctttttctaa atgacgttct
     2761 gctttacggc gaatatatgg ctctgcgatt tgacgcattt caatcgaagt ttgcacgcgt
     2821 gtcggtacac ccacgttttc caacgtatcc tgtaaagcta aggcattcat aactgtagct
     2881 aacataccca tgtaatcagc ttgagcacgt tccatgccca tttgtgctcc gatttgtccg
     2941 cgccagatat ttccgccgcc cacaacgatt gccatttcaa cgcccagctc gtgaacttct
     3001 ttgatttctt ggatgatttc tttgattact ggtggtttga ttccaaatcc tgcgtcccca
     3061 gctaaagctt ctccactcaa ttttaagact acacgctgat atttcggtgt gaccatgttc
     3121 attcctccaa tattttctat tgccatttta ccatacttag atgatttcga cactctaaga
     3181 ctggtttttc ttgttttgct taaaaaaagg gagcgcacaa accgtgcgtt cccgtctgtt
     3241 attcaacaga attatttttt cacttggctc ataacttctt ctacaaagtt atcttcgcgt
     3301 ttttcgatac cttcgccaac ttcgaaacgt acgaatgttt taacagttgc gccttttgat
     3361 gcaacgtatt tttcaacagt catatcagga tctttaacga atggttggtc caccaaagcg
     3421 atttctgctt tgaatttctt caagcgtcct tctaccattt tttcaacgat attcgctggt
     3481 ttgccttcgt tcaatgcttg ttctgtaaga acagtttttt catgttctaa ttcagcttct
     3541 ggaatttgtg attcgtttac ataacgagga ttgatagctg ctacgtgcat agctacgtct
     3601 cttgcaactg tttcgtcagt tgttccatct agaacagtca atacggcaat acgtccgccc
     3661 atatgcaagt atccaccaaa tgcagcattg tcgtcttttt ctacaacttc aaagcgacgg
     3721 aagctgattt tttctccgat aacttgagtt gcttcgatca agtcagattc aattgtacct
     3781 ttatctgttt tgattttcat tgcagcttcc atgtctgctg gtttgttttc agcaacaagt
     3841 tctgcaattt cttttactaa atcttggaac atttcgtttt tagaaacaaa gtctgtttct
     3901 gagtttactt caacgattgc tgcagtgtta cctttcacag caactgaagc caatccttca
     3961 gctgcaatgc ggtcattttt cttcgcagcc tttgccattc ctttttcacg taaaagatca
     4021 actgcttttt ccatgtcgcc ttctacttca actaatgctt ttttcgcatc catcattcct
     4081 acgccagtca tttcgcgtag ttcttttacc attttagctg taacgtctgc catgtttaac
     4141 tcctcctgtg tgtttttctt tttaaaaaaa gctgcttcaa aggagagtga ggcttgtcgc
     4201 ccagcctttg aaacagctcc atcattcaaa attattctgc tgaagaattg tcgccttcaa
     4261 caacatctac gatttcttcg attgatgttg ctgtgttttc ttctgtgaaa tcttcttcaa
     4321 ctacttgatc ttcaccttgg tttccttcga tgaaagcatc agccattttt gaagtaatca
     4381 atttaacggc acgaattgca tcgtcgtttg aagggattac tacgtcgatt tcatctggat
     4441 cgcagtttgt atcaaccata gctacgattg ggatatttaa tttgtgtgct tcttgaacag
     4501 caatacgttc tttacgtggg tcaacgatgt acattacatc tgggattctt ggcatatcag
     4561 cgataccgcc taagaatttt tcaagacgtt cacgttcttt gttcaaacca gcaacttctt
     4621 ttttaggtag aacttcaaaa gttccatctt cttccattgc attgatttgt ttcaaacggc
     4681 tgatacgttt ttggatagta tcccagtttg tcaatgttcc gcctaaccaa cgatggttaa
     4741 cgaagtattg tcctgcacga gttgcttctt ctttgattgc ttcttgtgct tgtttttttg
     4801 tacctacgaa taatgctacg ccgccttctt cagcaacgct cttcatgtaa tcataagctg
     4861 catctactaa tttaactgtt ttttgcaagt caatgatgta gattccgttt ctttctgtga
     4921 agatatattt cttcattttt ggattccagc gacgagtttg gtgaccaaag tgtacgccgg
     4981 cttctagtaa ttgtttcata gaaattactg ccatgtttgt ttcctccgat ttggttttat
     5041 ttttcctctt cttatcttct accttgccgg caaacttttt caagcaccta ccgacaatcc
     5101 aataagaatg tggatttgct gatgcataaa gcaccctcag ctattataca gacaaaagat
     5161 ccgtatcgca agagcaaata tacttttctg ctcaacattc aatagtttac ctgaaagata
     5221 aaaagaattc aagaaaaaca gtagatttct ctttgtttct ttattatcct ttttccataa
     5281 aataatttta ggtataaaag acgaatatta ttgggcaatc cttctttttt ttgttatgat
     5341 acatgtgaaa tctttcgtaa aggagaaaga aaatgcgcaa agctcaaaga catttgctga
     5401 ttaaacaagt cattgaagaa ttcccgatcc gtacgcaaga agaactatta aacaaacttg
     5461 ggcattatgg cgttaccgcc acgcaggcaa cgatttctcg agatatccgt gatctaaaaa
     5521 tcgtgaaggc acctgatgaa aatggcgttt cccgcttcgt attgtttcgc gaaaaaggaa
     5581 ctcctgagac taggactgag gaagaggatc ggctggttca aatgattgaa gacattgtat
     5641 tgaaagtcga acgagtccac tttttgacga tcgtcaatac cttgccagac aatgctcatt
     5701 tatttgcatc tgttttagat gagatcaaac ctccctctat tgtcagtaca attgctggat
     5761 tcgatacgat catggtcatc tcggaaaatg aggaggacgc aaaacgagta gaagaatttc
     5821 ttcaccatcc aactgaagta tcactttttt aatcagagca cgtattccta atatggaatg
     5881 cgtgtttttt tattttcaat attttcctta ttgaataaac ctttacactc tttacaaaaa
     5941 tacatcattt tttattcaaa tttttcaaca ccttgattga atcacaataa tcatcattta
     6001 tattggatgt taacaagagc agcttcttgc tctttgtgta tttcaaaaaa gaaatgaaag
     6061 gaaatcatat gtataaatta ttaacttctg gaccattgac gacttctgaa acagtgaaaa
     6121 atgctatgct gatcgatcat tgtacttggg atcaagaata caaagaaatt acacaatgga
     6181 taagaaaaaa attattggat cttgctcaag tatcggaagg aatatacaca acggtcttaa
     6241 tgcaaggtag cagctccttc ggggtagaat cggtattaag ttctgtgatc aggccagaag
     6301 atactgtact gatttgttca aacggcgcat atagacaacg aatgatcaac atgtgcgaat
     6361 gtctgaaact gaactatacc gtttatgagg tatcggaaca tcaaatcctc caagctcatg
     6421 tgattacaca gctgattgaa gagaatccgt ctatctccgc actagcaatg gtccatagcg
     6481 agactacttc cggtattttg aataatttgg aagagattgc tgctgtcaca caagaaagag
     6541 atcttttgtt tatagtggac gcgatgtcaa gctttggtgg gataccgatt tgtgtagccg
     6601 aactagggat tgattttttg atttccagct caaacaaatg tattcaaggt gttccaggct
     6661 tttcttttgt acttgctaat aaagcggaac tggaaaaacg aaaaaatgtt agccgaagtt
     6721 tgtcacttga tctatatgac cagtaccgcg tgatggaaaa cgagaatggc aaatggcgtt
     6781 tcacttcccc tacccatacg gttttggctt ttcaacaagc gctgaaggaa ctagaggcag
     6841 aaggaggaat cgaaactcga tattcacgtt atctcgccaa taatcatgtg ctacgacaaa
     6901 aaatgacaga gatggggttc cagcctttca ttcaagaata tcaggggcca ttcatcacct
     6961 cttttcttta ccctaaggac tggcgatttg attttaatca tttttatcag tatttaaagc
     7021 aaaatggatt tgtgatctat cccgggaaaa tcagcgatca tgaatgtttt cgtatcggaa
     7081 atattggcga gatctatccg gaagatattt tggaactgac agcaattatc aaagactata
     7141 aggagaacgt taatgattaa aactgtaatt tttgattggg caggaacaac tgtggacttt
     7201 ggctgtatgg ctcctgtcca cgcttttaga aatgcttttc tagaaaaagg aatccaactg
     7261 actgacaaag aaatccgcta accgatggga aaattaaaat gggatcatat ccaacagctt
     7321 ctttccctcc cttctgtcaa aaaacaatgg atagctattt atggacaact acctcaggaa
     7381 aaagaggtgg aggaattgta tcaacaattt gaacattatc tgtttaaaga tttggtcaag
     7441 cattctacct tgaaacccga cacattggaa acgatcgaac ggttgagaaa cgctgggatc
     7501 aatattggtt ccacgaatgg atttaatgct cctatgatga caatcgtagc agaaaccgca
     7561 aaaaaagaaa ggtattcacc agacttcatg gcaacttttg aagacgtcga agggtatggt
     7621 cgaccgtttc cttatatgat ccataaaaac atgcagtact tcaaaaacaa gtcggttgat
     7681 gaagtaatca aagtcggaga tacggtctca gatatccagg aagggaaaaa tgccggagtg
     7741 ttgacagtag gtgtaattga aggtagttcg ctaatgggga tttcttatga tgagtaccaa
     7801 aaattagaga tggggaaaaa gatcgtttaa aacagtctgt tgctcttaga ttccaacaag
     7861 ccggcgctga tatttgtgta aacaatttaa aagagttaac agaatacatt ttgtacgctg
     7921 acatgaatcc agaatgactt ttatacaaaa taagaggttg tgaaaaagct gtcctgtatc
     7981 aagtaataaa aacagccaag caaaaatagc tcctcatatt tttcgtttgg ttaggcttat
     8041 taccgcagat gcaagctttt gaacaccgtt tattcgaaaa aaaaaaggga gctgtgacac
     8101 gacttttgtc acagtcccct tatttgcaaa caaacagtgt ttaagagaca acctctccac
     8161 taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa
     8221 gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt
     8281 ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac
     8341 agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt
     8401 cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac
     8461 tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt
     8521 aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa
     8581 tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt
     8641 ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt
     8701 tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt
     8761 ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa
     8821 tacttcttct aataagacga tctttccttt acgagttgat aattttttac caccttgcgt
     8881 gatcaaacca aatggaatat ggtgcatgtc gtcagaccag tcaaagccca tttccttcaa
     8941 gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg
     9001 tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag
     9061 tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac
     9121 gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc
     9181 atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag
     9241 atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat
     9301 agcctcttta tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc
     9361 tggttctgtt tcagctactt catggaattg gacgtataaa cgaagcaatt cattgattgg
     9421 ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa
     9481 ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat
     9541 aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt
     9601 ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga tgttgctatc
     9661 cccataatgt tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt
     9721 attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc
     9781 gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa
     9841 tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg
     9901 ctcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag
     9961 atctttatta ttcatcttta ttcctcctaa agtgttccct tgattgttaa acaaaaaaaa
    10021 tctctagcga cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac
    10081 tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa
    10141 aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga
    10201 attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa
    10261 ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttcataa aaaaacaatc
    10321 cagatggcct aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg
    10381 aaagacttaa caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc
    10441 tcgatatttt ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca
    10501 atagctgctt ctactttcgg aagcatgctt cctggagcga attgcttttc atcaatgtat
    10561 tgtttcattt ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg
    10621 acatacacat tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct
    10681 aatttttctg aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct
    10741 gtttctacaa caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc
    10801 agcgtttcga tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt
    10861 ctccatccac gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct
    10921 ttggcttctt cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga
    10981 tcattttcat ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt
    11041 tccttcagta cttcgcccat ggcgttttgt aaccagtaac cgatagatcc ttctgtcatt
    11101 gcaacacatg tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga
    11161 atcaataggt taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt
    11221 tcgataaatt tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca
    11281 cttgcatccg tagacaagat ggcattgccc cctaaagcga caactacttt tcgtttggcc
    11341 atttcattcg tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt
    11401 tttgtgattt tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa
    11461 acgcgaggga taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata
    11521 cgattttctg cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact
    11581 tccatttcag tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca
    11641 tggaaagctg gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc
    11701 atttccatat tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct
    11761 tcacccattg atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca
    11821 acatcgtcgg taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg
    11881 acgatttctt cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa
    11941 attgcacctg tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca
    12001 acagtgatac cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt
    12061 tgtgttggat gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct
    12121 agttcctcta ccattttttg actgaatcca cggaattcga ttccgtcaaa catacgtcct
    12181 agtactttag ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct
    12241 agatattcag gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt
    12301 gtagatgttt tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata
    12361 ccacgttttt tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct
    12421 tttgtaaagt ctttttctgc taataagctt cttccttgga atactgattc tttcatatta
    12481 aacattctcc tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga
    12541 cagtaagact gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc
    12601 atgcagcgtg gaccaccacg acctcgtgac aattcacttg aattgatttc taacactttg
    12661 atgccatagc tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca
    12721 cctggagcga ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc
    12781 tcatctccat taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc
    12841 gtttcgtgta agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta
    12901 aagacatcca tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga
    12961 tctaccattg taaatacggt atccaaatgc atcattgcac ggttgttcgg aattttgatt
    13021 gccaacactt tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa
    13081 gctttcgcat tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat
    13141 tcgtctccgc cttcgatatg atctggattt tcacgatcca accatacttc gattccttta
    13201 tttgcaaatc ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt
    13261 gcttcaaaag tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt
    13321 gtaaagtaca agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga
    13381 tcatatagac tgctagaacg tacttcgata tcttttgtac gaaccccagc catgatttta
    13441 tctaccattt cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca
    13501 ttcgatgcaa tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct
    13561 gcgtcaatcg cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc
    13621 aatgtcttcg caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg
    13681 aacaacagac gatccataat atctggtgtc aaattttcca cttcttgtcc aggccgtttc
    13741 agtaaaactg tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg
    13801 tgttgcctcc ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat
    13861 aattttgtct cattcaaaat gagacattcc tcacattaat attcgataat taaataatta
    13921 aacgtttttc tatttttaag cgtaatttat tcatttttta ttctgtatac actttcaaaa
    13981 aaatatttat tcattgaaaa aatttttttg aataaattaa aaaaataaat tatattat
//
LOCUS       DACDTO010000075        13919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDTO010000075 DACDTO010000000
VERSION     DACDTO010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.075"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(221..1423)
                     /locus_tag="IWK70_002187"
     CDS             complement(221..1423)
                     /locus_tag="IWK70_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9614699.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="IWK70_002188"
     CDS             complement(1426..1620)
                     /locus_tag="IWK70_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9614700.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="IWK70_002189"
     CDS             complement(1667..2515)
                     /locus_tag="IWK70_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP9614701.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="IWK70_002190"
     CDS             complement(2720..3070)
                     /locus_tag="IWK70_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614702.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="IWK70_002191"
     CDS             3665..4111
                     /locus_tag="IWK70_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9614703.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="IWK70_002192"
     CDS             4735..5727
                     /locus_tag="IWK70_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9614704.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="IWK70_002193"
     CDS             5743..6627
                     /locus_tag="IWK70_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9614705.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="IWK70_002194"
     CDS             6676..8283
                     /locus_tag="IWK70_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9614706.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="IWK70_002195"
     CDS             8284..9768
                     /locus_tag="IWK70_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9614707.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="IWK70_002196"
     CDS             9945..10937
                     /locus_tag="IWK70_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9614708.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="IWK70_002197"
     CDS             10989..11858
                     /locus_tag="IWK70_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9614709.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IWK70_002198"
     CDS             complement(12278..13843)
                     /gene="guaA"
                     /locus_tag="IWK70_002198"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9614710.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4258 a         2358 c         2875 g         4428 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt tggctcttcg tcaaatcgtg ttggtgaaga caaatttgaa taaaacttag
    13861 tccgatatgc ggcgcgttct gtgcgccgca tattttttta gttttcaaag atcagacct
//
LOCUS       DACDTO010000076        13787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDTO010000076 DACDTO010000000
VERSION     DACDTO010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.076"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            206..544
                     /locus_tag="IWK70_002199"
     CDS             206..544
                     /locus_tag="IWK70_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614711.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(749..1324)
                     /locus_tag="IWK70_002200"
     CDS             complement(749..1324)
                     /locus_tag="IWK70_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9614712.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2056..2637)
                     /locus_tag="IWK70_002201"
     CDS             complement(2056..2637)
                     /locus_tag="IWK70_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9614713.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2820..3446)
                     /locus_tag="IWK70_002202"
     CDS             complement(2820..3446)
                     /locus_tag="IWK70_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9614714.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3468..4799)
                     /locus_tag="IWK70_002203"
     CDS             complement(3468..4799)
                     /locus_tag="IWK70_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9614715.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4954..5355)
                     /locus_tag="IWK70_002204"
     CDS             complement(4954..5355)
                     /locus_tag="IWK70_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9614716.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5441..5647)
                     /locus_tag="IWK70_002205"
     CDS             complement(5441..5647)
                     /locus_tag="IWK70_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614717.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5742..7244)
                     /locus_tag="IWK70_002206"
     CDS             complement(5742..7244)
                     /locus_tag="IWK70_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9614718.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7262..7717)
                     /gene="argR"
                     /locus_tag="IWK70_002207"
     CDS             complement(7262..7717)
                     /gene="argR"
                     /locus_tag="IWK70_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9614719.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7815..8432)
                     /locus_tag="IWK70_002208"
     CDS             complement(7815..8432)
                     /locus_tag="IWK70_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614720.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8653..10026
                     /locus_tag="IWK70_002209"
     CDS             8653..10026
                     /locus_tag="IWK70_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9614721.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10101..11213
                     /locus_tag="IWK70_002210"
     CDS             10101..11213
                     /locus_tag="IWK70_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9614722.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11285..12922)
                     /locus_tag="IWK70_002211"
     CDS             complement(11285..12922)
                     /locus_tag="IWK70_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9614723.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13090..13698)
                     /locus_tag="IWK70_002212"
     CDS             complement(13090..13698)
                     /locus_tag="IWK70_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9614724.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3726 a         2895 c         2469 g         4697 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      121 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      181 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      241 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
      301 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
      361 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
      421 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
      481 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
      541 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
      601 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
      661 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
      721 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
      781 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
      841 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
      901 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
      961 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1021 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1081 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1141 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1201 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1261 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     1321 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     1381 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     1441 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1501 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     1561 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     1621 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     1681 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     1741 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     1801 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     1861 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     1921 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     1981 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2041 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2101 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2161 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2221 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     2281 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     2341 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     2401 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     2461 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     2521 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     2581 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     2641 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     2701 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     2761 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     2821 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     2881 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     2941 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3001 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3061 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3121 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3181 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3241 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     3301 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     3361 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     3421 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     3481 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     3541 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     3601 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     3661 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     3721 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     3781 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     3841 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     3901 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     3961 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4021 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4081 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4141 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4201 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4261 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     4321 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     4381 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     4441 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     4501 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     4561 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     4621 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     4681 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     4741 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     4801 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     4861 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     4921 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     4981 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5041 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5101 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5161 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5221 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     5281 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     5341 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     5401 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     5461 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     5521 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     5581 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     5641 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     5701 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     5761 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     5821 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     5881 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     5941 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6001 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6061 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6121 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6181 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6241 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     6301 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     6361 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     6421 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     6481 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     6541 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     6601 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     6661 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     6721 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     6781 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     6841 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     6901 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     6961 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7021 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7081 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7141 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7201 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7261 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     7321 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     7381 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     7441 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     7501 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     7561 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     7621 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     7681 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     7741 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     7801 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     7861 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     7921 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     7981 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8041 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8101 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8161 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8221 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     8281 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     8341 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     8401 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     8461 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     8521 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     8581 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     8641 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     8701 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     8761 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     8821 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     8881 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     8941 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9001 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9061 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9121 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9181 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9241 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
     9301 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
     9361 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
     9421 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
     9481 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
     9541 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
     9601 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
     9661 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
     9721 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
     9781 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
     9841 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
     9901 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
     9961 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10021 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10081 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10141 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10201 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10261 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    10321 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    10381 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    10441 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    10501 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    10561 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    10621 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    10681 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    10741 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    10801 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    10861 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    10921 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    10981 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11041 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11101 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11161 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11221 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    11281 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    11341 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    11401 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    11461 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    11521 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    11581 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    11641 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    11701 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    11761 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    11821 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    11881 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    11941 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12001 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12061 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12121 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12181 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12241 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    12301 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    12361 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    12421 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    12481 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    12541 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    12601 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    12661 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    12721 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    12781 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    12841 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    12901 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    12961 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13021 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13081 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13141 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13201 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13261 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    13321 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    13381 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    13441 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    13501 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    13561 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    13621 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    13681 tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct
    13741 attaacttta tcatttagaa atatttcata ggtaaaagaa acatgac
//
LOCUS       DACDTO010000077        13740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDTO010000077 DACDTO010000000
VERSION     DACDTO010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.077"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            298..1083
                     /locus_tag="IWK70_002213"
     CDS             298..1083
                     /locus_tag="IWK70_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9614725.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            1096..1947
                     /locus_tag="IWK70_002214"
     CDS             1096..1947
                     /locus_tag="IWK70_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9614726.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(2071..2523)
                     /locus_tag="IWK70_002215"
     CDS             complement(2071..2523)
                     /locus_tag="IWK70_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9614727.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(2988..3356)
                     /locus_tag="IWK70_002216"
     CDS             complement(2988..3356)
                     /locus_tag="IWK70_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9614728.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            3506..3988
                     /locus_tag="IWK70_002217"
     CDS             3506..3988
                     /locus_tag="IWK70_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9614729.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            4025..5035
                     /locus_tag="IWK70_002218"
     CDS             4025..5035
                     /locus_tag="IWK70_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9614730.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(5150..5863)
                     /locus_tag="IWK70_002219"
     CDS             complement(5150..5863)
                     /locus_tag="IWK70_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614731.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(6053..6643)
                     /locus_tag="IWK70_002220"
     CDS             complement(6053..6643)
                     /locus_tag="IWK70_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9614732.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            7127..7354
                     /locus_tag="IWK70_002221"
     CDS             7127..7354
                     /locus_tag="IWK70_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9614733.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            7370..8536
                     /locus_tag="IWK70_002222"
     CDS             7370..8536
                     /locus_tag="IWK70_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9614734.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            8770..9813
                     /gene="hrcA"
                     /locus_tag="IWK70_002223"
     CDS             8770..9813
                     /gene="hrcA"
                     /locus_tag="IWK70_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9614735.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            9907..10470
                     /gene="grpE"
                     /locus_tag="IWK70_002224"
     CDS             9907..10470
                     /gene="grpE"
                     /locus_tag="IWK70_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9614736.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            10521..12350
                     /gene="dnaK"
                     /locus_tag="IWK70_002225"
     CDS             10521..12350
                     /gene="dnaK"
                     /locus_tag="IWK70_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9614737.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            12501..13667
                     /gene="dnaJ"
                     /locus_tag="IWK70_002226"
     CDS             12501..13667
                     /gene="dnaJ"
                     /locus_tag="IWK70_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9614738.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
BASE COUNT         4613 a         2304 c         2889 g         3934 t
ORIGIN      
        1 ggtgataaat aagcccagga aaggggctgt gacaaaagtc ttgtcacagc ctttttttcc
       61 aaataaatgg tttttcagaa gtagcttctt cggaaataag ccgaaatttc ttgaagctga
      121 ccacttctgt cacaacctct ttctttatag aaataaaagg acactagcaa ttcatgaaaa
      181 cgattgctag agcgtatcat ttgctttaga ctatatatct tggtgtacac tcactttaag
      241 gcggaaataa aagttatccg taataacagg tttaggtatg aaaaaggagt gggtactatg
      301 tacaaggaat tagaaaacaa agtcgcagta gtaactggtg gctcaaaggg gattggaaca
      361 gcgattgctc aacgattcgg gaaagaaaaa atgaatgtag ttgtcaacta taactcggat
      421 aaagaaggag cagaaaaagc agtcaaagca atcgaagagg caggcggaaa agcagtctct
      481 gtacaagcag atgtcagttc tgaagaagga atacagcgtt tacttgatac ggctttagaa
      541 aattttggga cactggacgt ttgggttaat aatgctggga tggaaaatca gcatccaacc
      601 catgaactta gccttgaaga ttgggaaaga gtgttaaaag tcaatttgac tggcgttttt
      661 atcggaacga aagcggcttt aaattacttt acagagaata ataaaaaagg aaatatcatc
      721 aatatgtctt ctgttcatga acagatccca tggccaacgt ttgctcatta cgctgcttca
      781 aaaggcgggg taaaattatt taccgaaacg gtagcaatgg agtatgcacc gaagggaatc
      841 agaatcaata gtataggccc tggtgctatc cgtacaccta ttaatgcggc aaaatttgat
      901 gatcctgaac aaaaagaact cttggaaagt atgatcccac taggacgcgt aggtgaacca
      961 aaagaaatag cggcgacagc tgcatggttg gcttcagacg aatccagtta tgttacaggt
     1021 attacactat ttgcagatgg tgggatgact ttatatcctt ctttccaagg tggaaaagga
     1081 taagggtgaa taaaaatggg tttattgatt gcattgatcc cggcagttgg ctgggggatc
     1141 cagccactga ttttgaaaaa gattggtggc agaccaacaa atgagatatt aggaactggt
     1201 attggagcgg tgcttgtcgg cttactcgtt caattattcg cttctccagc tggtatttca
     1261 gtgactacat ttttgattag tttactttct ggtgcatttt gggttattgg acaaacagga
     1321 cagtatacat ctttcaactt gattggtgtt tctaaaacga tgccaatttc gactgcatta
     1381 cagttagtcg gaacctcgtt gattggtgtc ttcgcttttg gagaatggtc tggaagtatg
     1441 gggaaaatca ttggagcagc agcaatcatt cttcttgtat ttggttctgc tttaacggca
     1501 atcagtgatg atcctggaaa aaaaggcggt atcacaaaag gaattagtct attagcatca
     1561 acaagtattg gttattgggt atacagtgca ttgcctaaac tagttgatgc aagcggactt
     1621 tccattttct ttccgcaaat gatcggtgtc tttttagggg cagtgattta tgtgattttc
     1681 cgtaaaccaa ccgcattttc tgaagacaag agttggaaag caacgatcgt tggagccatc
     1741 tttagtgtgt cagcattggc ttatattttt tcagcaaatg aaaatggggt tgctactgca
     1801 tatatcatta cacagctaaa cgtagttatc tctacattag gcggtatgta catcctgcat
     1861 gaaaataaga caccaaaaga aaaaaaatta acgattatcg gattattgct gatcgtagta
     1921 ggaagcatgg tcacggtctt tatttgagga taaagatggt aaaatttcat ttattaaaaa
     1981 aatatttgtt tgaaccaaac agtaagtact tataaaaaaa tagcgctaga atttccaaca
     2041 tctctggaat ttctagcgct ttttaattgt ttaagataca agggaaatat ctgtctggat
     2101 caataatttt tcattgatcc attgactaaa gtttacgaca taattgaagt taccaccgat
     2161 tagtatctcc gcatgattgt ctttataaag atcgtattgt aaaagacggt cttttttatt
     2221 tgcgtaaaca tcgttatggt actggactaa ttgttctcgg aattgattga ttgctgtaaa
     2281 atcacgatat tgaaggttga aacgcatcgt gattggtgca tttagcatga atcgctgcat
     2341 ttcttttgct gcttcctctg cttgatctcc gtataggaac tcaaaaagat tacgttcaga
     2401 aaatttccat tgcccgccaa cctttgttcc ttttagtttt ccttcatgaa gataatttct
     2461 gatagaacgt gtagtaacac ctaattgttc ggctaattca tttactgagt aaaaatcttt
     2521 caatgttcac cctcctatgt gaatggttct actgacttag ttgatatttg cttaatcacg
     2581 aaatgtagaa agaacgattt taattaaggt cattgtaaaa atcaagtcag aaaattctaa
     2641 cttttatttc tctaaagaaa ttcgttctct cctaaagtga tattatattt aaatatacct
     2701 gaaaataatg ggcagtatta tgaaagatca ctgatatttt ctcgtatttt ttccttattc
     2761 aaaagttttg caggaaaatt gctgaaaaaa taattttact atgtagaaaa ttcaaagcca
     2821 ttcaatcagt gatttttttc agcacaagaa agactcctta taaaaatagc agatactttg
     2881 tcaattaagt gtttaaccag ggatgataaa aaggatagga ttgaacaaaa cctgtataag
     2941 gtgatgaata gtttcctcgc cagtaaaagg tttattcgta taaagcttta tcgccaaaat
     3001 ttatcatgac cttttttatc ttcaatatta aatgaaatca attgttcaat caattcccag
     3061 ttgattacac ttttccaagg aactttgatg acgttatccg tatatacgta gcctgcttct
     3121 tggatatttt tttcaagggc acgaatcgct gctttttccg gtgcgataga aaaatgattt
     3181 ttagcaaaac taaatgcaat aataaaggta ccatggtcag taaacatagg ttggttccat
     3241 tttacagttg tgtctagctg gggatacgtt tctgttatct tagcaaaaag gtctttgatc
     3301 ttttgttgat gttctttttc tttgatttga ttgatgatcg gctgaaattt ttccattcta
     3361 aaacgcctcc attaagtggt ttgatttatt ttaccagaaa aacaatagtt atgtataaaa
     3421 aacgaacaaa acacaagtaa tgttgtaacc gctatattta tatgataaga tgtctcaaat
     3481 aataaaatag aaaagagaca taaaaatgat tgacttggca aagaaattct tacacgaaaa
     3541 tatatatttt gagttgtatc atcatcgagc catttataca aatgaagatg ctttggtaat
     3601 aaagaaagaa caaggattta caggtacaga gaccaaaagc ttattcttaa aagataaaaa
     3661 agataataac tacgtatatc tgacttttac tacgaaacaa acggatttca aagcattagg
     3721 gaagatcgtc ggtaaaaggc tttctattgt ttcatcagaa cgcatggaag aacaaacggg
     3781 acaaaagcag ggcgctgttt ctccgtttgg ttatgaaaaa cagattccga ttattattga
     3841 tgaagagctc ttatcaaatg aaaaacttgt ttttgcacca ggtagaccag atcaaacgat
     3901 ggtcatccaa acggttgact taaacaaaat catccagcta cttgagatgc cgatattttt
     3961 gatgccggca aacgaaaaaa acaattaacc aagtaaaata gtgcatactt ggaggtgaag
     4021 caacatgata agttatccat tattagaaag aaatgcaacg attggggtta ctgcaccatc
     4081 ctcaggcgta tcagaggaac accattcgtt actcgaatca gctttaaaga gaatggaaga
     4141 aaaatctttc caagtagtta ctggaaagac gctttggacg caagataagg aaaaatcagg
     4201 tccagcaaga aaacgagcag atgaattcaa tcaaatgatg caagatgaaa atatccaaat
     4261 gatctttccg acttggggag gagaattatt gattgagatt ctcgattttg tcgattttga
     4321 tgctctacaa ccaaaatgga tagtaggtta ttcagatatt agtacgttgc tacttgctgt
     4381 cacattaaaa acagggatag ccactgcaca tggaactaac cttattgatt taagaggaga
     4441 gtacttggat gaaacgacag caatgtggga aagtgtactg aaaacaagag aaggagaatc
     4501 gattctacag caatcttctg aaaagtatca aaaagtgtgg ccccatgagc attcatcatc
     4561 ttctgttttc catctcactg aaccaacaaa ttggcaatca gtcagcggag atgcagtaag
     4621 gatgaaggga cgattattag gtggttgtat tgatacgatc aggcatttaa tcggtactcc
     4681 ttatggagat gtccaatctt ttaaaagaaa gtataatgat ggaaagccga tcttgtggtt
     4741 tctagaaaac tgtgagctat cgtcagttga tttaagaaga tcacttgttc agatgaaact
     4801 ggcaggctgg tttgatggca gctcggggat catgttcggt agaagtgcgg cagaggattt
     4861 gttagattat aaaatgaagg atgtatacga agatctgtca aaggaattaa aactaccgat
     4921 cttgtatgat attgattgtg gtcatgtacc gccacaactg acattgatta atggtgccta
     4981 tgcagaagta gcggtagaag atagaaaagc gactgttttg caaacgttta tttaaaaaat
     5041 tttttcctgc cttggagtct atcaaagacc aataaatcta aatagaaaag aaagtgaaag
     5101 aagctgacaa aatgctgtca gcttcttttt gtataacata ttagaaagtc taattatcta
     5161 taactgtcac atcgattaac tgtttcacgt cattgggatt tcctgtagga atagctacta
     5221 cagatgcatt atcaatgatt tcttgagttg tttctctaat gaacgctgga taagatgttt
     5281 gatctgcagg catttgccaa tgtgaaggaa ctggataact agtgattgtc ccgtcatggt
     5341 tacttgaata cactaaaact ccctggtaac tgtatttccc agtgagcata accacatctt
     5401 tcggatagac tgcactccct gcatcataag gggcgatagg tgttcctgca ggataatgaa
     5461 taacatgtaa ttcgaaatca cccgatgtat ctctaaaagt attacctcta atagctagcc
     5521 aaacacgtgc gtactcaatt tgtagatcag aatatcctgc taacggatct attgactgag
     5581 aagactgtga ggaggttgtg tctattgttg aagaagaaat atttgtctca actgtactac
     5641 tagatgtaga agaagtattg ggctctgaga ctttactgct tgttgaagat gtattcgttt
     5701 cagtagaagc tgatttctga gtacttgatt tttcagctga actggtttgt ctgattgaac
     5761 ttttgtctgt ggtcgcagat ggatcactat tttctttact aggagacgta catcctacaa
     5821 ggaataaaga gaccaacaat aaaataaaac ctaacttttt catataaaat acctcattat
     5881 ttttttaata ccagtatagc ataatcctga atttttgaat ccgtaaaacc ttgtctatga
     5941 atataaatag agtgtatctt ctttgataaa tgtcagaaaa agtgtgacaa ataatcatca
     6001 gtaaaattac ttgtcacact attgataagg agtatttaat tgaagagaat gattaggaga
     6061 aaggatctcc gtacttttta tagtaatcat agaatccaag tttgtataga cgaatcgttt
     6121 tttctcttat ttcttctgtt tcaaaatgga ctgcttgaag aatcaagtct gtgaattcta
     6181 taggagctgt tccattcgca gtaatcaaat tacgatcttt cacagcttgt tgttctataa
     6241 aatcttcttt attctgataa ttttccgatt ctaaccaagg aaattgcgca tttccggtgt
     6301 gtttgtaacc ttgcaataat ccgctctgag ctaaataatc aactgcccca caaatagcag
     6361 ctactggtac gtctgttttt aacaatttat cgattatttg atataaatga tgatttttta
     6421 tgtcccatga attgccaccg atcataataa aaaggtcaac tttttcaggt atttgttcga
     6481 ttaagtaatc gatttttgtt tttaatcctc caatggaagt gacctctgat tggatagaag
     6541 cagttttgtt ttgccatttc tcattttggt ttagtgtgct agtaatataa gcaccttccc
     6601 aatcggcata ttcctctaat agaaacgtaa tcgcagtttt cattttcaag ctctccttta
     6661 ctatctataa caaggacaac agttttaacc tacagacatt cattaaaatt gggtagcgac
     6721 agcgaaaaca acaattctta agttttagac agagtataca gatagtcaac ttaattaaat
     6781 acgaactaaa taaaaatgac aggtaatcat ttttgtttac cagcattata acattttgca
     6841 tacatggatg acaaaagaac caattcccac ttgcttgagt acaatattaa aagaaactgg
     6901 aaggcaaaaa gtaattatag aaacgaaaaa taaatcatat cgggcactca gacatgaaaa
     6961 caaccctaga gatatataac taggtaacca gtttaatgaa agaaaaaatc atccaagaag
     7021 tcgattcttg gattttttag tggatttgtt cgccaaatac gtttagtatg cgtatatgag
     7081 aggttgtaag caaagcgttg tggtctgagc acttaaggag gaatctgtga aaatagcttt
     7141 tcatattttt gcagattttg acttaatgcc gaaggtcagc tttgcgatta ccgtttatcg
     7201 agaggctgta aaagcaccgt gtagctccga tgacaataag caagcatttt tagagctagg
     7261 tttccttgat tctaaatatg cggcttattt caatgagttg gtgcttgaac gcgattttaa
     7321 gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa agaatttcaa tgatagaaac
     7381 aataaatccc gcagaaaaca aggtttctgc gtacattcat atcccatttt gcgagcatat
     7441 ctgctattac tgtgatttta ataaagtttt tttagaaggg cagccagtag atgagtatgt
     7501 ggaaatgctg ctgaatgaaa tgaaaataat gctagaaagt catccgacag cgaatttaga
     7561 aacactttac gtagggggcg ggacaccaac ttctttatct gctaaacaac ttgatcgact
     7621 gttatcaggt gctagagaga tcctgccttt tcaaaatggt aatgagttta ctgtagaagc
     7681 gaatccaggt gatttgaccc gtgaaaaact tcaagtaatg aaaaactatg gcgtcaatcg
     7741 tttatccatg ggagtgcaga catttgataa tcgcttattg aaaaaaattg gacgtaaaca
     7801 caccgcagaa gatgtgtatc aaactatgca atttttagaa gcagaaaatt ttaccaatgt
     7861 aagcatcgat ttgatttatg cattaccggg acaaacacta gaaggatatc tggatacttt
     7921 ggaaaaagct ttggctttgg atctgcctca ctattctttg tactcattga tcctcgaaaa
     7981 caaaacaatg ttcatgaact gggtacgaca aggaagactc gaactgcctg atcaggaaac
     8041 ggaaaccaga atgtttgaag agacgattga agcaatggaa aagcatggac gtcaccaata
     8101 tgaaataagt aactttgggc tctccggaca tgaatctcaa cataacctca tgtactggaa
     8161 caatgaccac tattttgggt ttggagctgg tgctagcggt tatcttggag atattcgtta
     8221 ccgcaacaaa ggaccaatcc aacactattt acgcccatta cgagcaaatg aacgcccggt
     8281 attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa gaagaaatgt tcttaggact
     8341 aagaaaaaaa acagggatta ctaaaaaaca tttctatgat cgctatcaaa aaacaattga
     8401 atcggtgtac ggtgatgtgc taagggaatt agagcaagaa gggttacttg ttaatgaaga
     8461 agaccgtgtt tatttgacac caagagggac ctttttgggg aacaatgtgt ttgagcgctt
     8521 tttgtttgaa aaatagttta aagaatattt gttttttatc tgaatttaaa taaaaaaagt
     8581 tcaaaaatac aataaaggac tattgaaacg ttgatataac tggatttaca tcaattattt
     8641 agaattagca ctcttgtgtt tagagtgcta aaaaatggag tctttctctt gacaggagaa
     8701 gggcttcttg ctatatttat aagtggatta gcacttagaa tatatgagtg ctaatgagag
     8761 gtgaggaaga tgttaacaaa gcgacaaaac gacattttac gtctgatcat ccaacactat
     8821 acgagtagtg gcgtgcccgt tggttccaaa acattgatgt ctgagggagt caaggccagc
     8881 tcagcaacga ttcgcaatga tatgaaagca ttagaagatg aaggattgct gttgaagact
     8941 cactcttctt ctgggcggat tccatctgct ttaggctatc gttattacgt ggatcatttg
     9001 ttaaaacctg ctcgcgtagc agaagatgat ttgctgctga ttcgtcagtc tctaggcaga
     9061 gaatttcatg aaatcaatga gattattaag caatctgctg agatattatc tgagctgaca
     9121 agctatacga ctttttcatt gggacctgaa ataaaagacc gccgactgac tggctttcgt
     9181 atcgtaccct tgaataatcg acagattatt gctatcgtcg taacagatat ggggaatgta
     9241 gaaagccaag tgttcactct tcccgaatat atgggaagtc aggaactaga aaaaatggta
     9301 cagattgtaa acgaccgtct agtcggagat cctctgatga cggtgtacca taaacttcgt
     9361 acggagattc cgatgattct tcataaatat ttccaaacga cagaagggat gtctcagtta
     9421 tttgacaccg ttcttggtca tgtttttgaa gataaggttt atgtcagtgg acaaatgaat
     9481 ctcttggatt atgagccccg tcaagatctt gatcagttca aatcgatgtt ttcttttatg
     9541 aaagactcag acggacttac acaaatgatc actccgatgg atagcaatat tcatatcaag
     9601 attggtgctg aattaggcaa tgatttattg cagaatatga gtatgattca agcaagttac
     9661 gagatagatg gtcatggaag aggtacaata gccttgttag ggcctactag tatgccttat
     9721 tctcggatgt tcggtctagt cgatgtttat cgaagagaac tagcagctaa acttgctgat
     9781 tactaccgtt cgcttgactt gtcaaactcg taatgtgaat aacgaagcta tttgcagctg
     9841 atcgttctct ctttcttagt aagagcaatc aaacagctga ctgaataatg aaaggaagac
     9901 tgtatcgtga aagaaaatca agaagaacta gataaagaaa tgacagatgc tcagccagaa
     9961 ccggaaatcg atgtcgaagc tgcagaagat tcgggtattt cagaagctga agcggaagaa
    10021 ttcgaaacag cgaaattaaa agctgagtta gaagaaatgg aagataaata cttgcgggct
    10081 cgtgctgaaa tcgcaaatat ggcaaatcga ggaaaaaatg agcgtgagca attacaaaaa
    10141 taccgttcac aagatttagc caaaaaatta cttccatcaa ttgacaacct ggaacgtgca
    10201 ttagccacag aagtgtcaga tgatcaaggg gcaggtttga aaaaaggcgt ggaaatggtc
    10261 ttggaaagtt tgcgtaacgc actggaagaa gaaggaattg aaaaaatccc tgctaaagga
    10321 gaagcctttg atcctaatct tcatcaagca gtacaaactg ttccagcgac tgaagataca
    10381 ccagccgata cgattgtaga agtactacaa gaaggctaca aattacatga tcgcgtgtta
    10441 agaccaacca tggtcattgt tgcacaataa ttgtgcggat ataaaactta cactttcaat
    10501 taaagaagga gacgttaatt atgagtaaaa ttatcggtat tgacttaggt acgactaact
    10561 ctgcagtagc agtattggaa ggaaacgaag caaaaatcat cgctaaccca gaaggaaacc
    10621 gtacaacacc ttctgttgta tcattcaaaa atggagaaat ccaagtaggg gaagttgcaa
    10681 aacgtcaagc tgtgacaaac ccaaatacga tttcatcaat caaacgccat atcggtgaag
    10741 caggttacaa agtagatgta gaaggtaaat catatacacc acaagaaatc tcagcaatga
    10801 tccttcaata tatcaaagga ttcgcagaag actatttagg tgaaaaagtc gaaaaagcag
    10861 ttatcactgt gccagcttac tttaatgatg cacaacgtca agcaacaaaa gatgctggta
    10921 agatcgctgg tctagaagta gaacgtatcg tcaatgaacc aactgcagct gctcttgcct
    10981 atggtttaga taaaacagat cgcgacgaaa aaatcttagt attcgacctt ggtggcggta
    11041 cttttgacgt atctatcctt gaactaggag acggtgtctt tgatgtattg tcaacagctg
    11101 gcgacaacca tctaggtggg gacgactttg ataacaaaat catcgactac atggtagcag
    11161 aattcaaaaa agaaaacagc attgacttgt ctcaagacaa aatggcttta caacgcttga
    11221 aagacgctgc tgaaaaagcg aaaaaagatt tatctggcgt atcaagcaca caaatcagct
    11281 tgccatttat cacagcagga gaatctggac cattgcattt ggaaatgaca ttgacacgtg
    11341 caaaatttga tgaattaaca gctgacttag tagaacgtac aaaagtacca gtacgtcaag
    11401 cgctaaaaga tgcaggttta tcacaatcag aaatcgacga agttatttta gtcggtggat
    11461 ctactcgtat ccctgcagtt gtagaagcag tacgtaaaga aacaggaaaa gaaccaaaca
    11521 aatcagttaa ccctgatgaa gtagttgcta tgggtgctgc tatccaaggt ggcgtgatca
    11581 ctggtgatgt gaaagatgtt gttttacttg acgttacacc attgtcatta ggtatcgaaa
    11641 caatgggtgg agtgttcact aaattgattg accgcaatac aacgattcca acaagtaaat
    11701 cacaagtatt ctcaactgcg gcagataatc aaccagcagt agacatccat gtattacaag
    11761 gtgaacgccc aatggctgct gacaacaaaa ctctaggaag attccaattg acagatattc
    11821 cagcagctcc tcgtggtatt ccacaaatcg aagtaacatt cgatattgat aaaaacggga
    11881 ttgtaaatgt atctgcgaaa gatctaggta cacaaaaaga acaaaaaatc acgatcaaat
    11941 cttcttcagg tctaacagac gaagaaatcg aacgtatggt aaaagatgca gaagcaaatg
    12001 cagaagcaga taaagctcgt aaagaggaag ttgacctacg taatgatgta gatgcattgc
    12061 tattctctgt ggacaaaaca ttgaaagaat tagaaggcaa agtggacgaa gaagaagtga
    12121 aaaaagcaga aacagctcgc gatgaattga aagccgctgt tgaagcaaac aacatcgaag
    12181 aaatgaaaac taaacgcgat gcattgaacg aaatcgttca aaacttgact gttaaactat
    12241 acgaacaagc ggcacaacaa caagctcaag aaaacccaga agcagctcaa ggcggagcag
    12301 atgatgtcgt tgatgctgat tttgaagaag tcgatggcga cgataaataa tcagttcact
    12361 gaatttcgag agaaggccaa agttgctact ttggcttttt cttttgaaaa atagtttgaa
    12421 gtatcggttt ttgtttcaaa caaaatatga tatgataaca agcgacgtga aaatgatgag
    12481 aaccaatagg aggaaagcct atggcaacaa aacgtgatta ttatgaagtc ttagggctct
    12541 caaaaggagc atcagaagat gaaataaaga aagcttatcg taagctttcg aaaaaatacc
    12601 atcctgacat caataaagaa cctgatgcgg aagaaaaatt caaagaagta tcagaagcat
    12661 atgagatttt aagtgacccg caaaaacgcg ctgcatatga ccaatacggg catgcaggca
    12721 cagatccaaa ttacggtgct ggtggagctg gtggcggatt tggtggattc ggcggcttct
    12781 ctggtggcgg cttcggtggt tttgaagata tcttcgactc tttctttgga ggaggcggtc
    12841 ggtcagtcga tccaaatgcg cctcgtcaag gtgctgattt gcaatatacg gtcgacttgt
    12901 cttttgaaga agcgattttt ggtgtagaaa aagaaatcaa gtataatcgg gaagaaatct
    12961 gtcatacttg tggtgggaac ggtgcaaaac caggcacaca accaactact tgtcataaat
    13021 gtcatggtgc aggtacgatt aatgtagaac gccaaacacc acttggacga gtgatgagcc
    13081 gtcaaacttg tgatgtttgt catgggacag ggaaagaaat caaagaacct tgtccaacct
    13141 gtcatggctc aggacatgag aagaaagcac attcagttaa agtgaatgta ccagctggtg
    13201 tagaagaagg acaacaaatg cgtttagctg gtcaaggaga agctggagaa aacggcggac
    13261 catttggtga tttgtatgtg gtcttccgtg tagaagaaag tgatatcttt gatcgtgaag
    13321 gttctgaaat ttactatgaa ttgcctttaa gttttgtaca agcagctttg ggggatgaag
    13381 tccaagtacc aactgtccat ggagatgtga aattgaagat ccctgctgga acacagacag
    13441 gtacgaactt ccgtttacgt ggaaaaggcg ctccgaagtt acgtggtggt gcaacaggcg
    13501 accaacatgt caaagtaaaa ctgatcacac cgaaaaatct aaatgaacaa caaagagaag
    13561 cattacgtgc attcgctgaa gctggcggca tcaaagttga agaacagcaa gaagacggtt
    13621 tcttcgataa gtttaaagat gcttttggcg ggaaaaagcg gaagtagttt ttctagtaaa
    13681 aacgctaagt cattaataat taaaaaccag acgaacaatc atatgttcgc ctggtttttt
//
LOCUS       DACDTO010000078        13731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDTO010000078 DACDTO010000000
VERSION     DACDTO010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.078"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..1452
                     /locus_tag="IWK70_002227"
     CDS             1..1452
                     /locus_tag="IWK70_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9614739.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            1801..2433
                     /locus_tag="IWK70_002228"
     CDS             1801..2433
                     /locus_tag="IWK70_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9614740.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3021..4460
                     /locus_tag="IWK70_002229"
     CDS             3021..4460
                     /locus_tag="IWK70_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9614741.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4755..4973
                     /locus_tag="IWK70_002230"
                     /pseudo
     CDS             4755..4973
                     /locus_tag="IWK70_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5222..5437
                     /locus_tag="IWK70_002231"
     CDS             5222..5437
                     /locus_tag="IWK70_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614742.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5654..5910)
                     /locus_tag="IWK70_002232"
                     /pseudo
     CDS             complement(5654..5910)
                     /locus_tag="IWK70_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6097..7008)
                     /locus_tag="IWK70_002233"
     CDS             complement(6097..7008)
                     /locus_tag="IWK70_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9614743.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7027..8031)
                     /locus_tag="IWK70_002234"
     CDS             complement(7027..8031)
                     /locus_tag="IWK70_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9614744.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8028..10151)
                     /locus_tag="IWK70_002235"
     CDS             complement(8028..10151)
                     /locus_tag="IWK70_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9614745.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10156..12603)
                     /locus_tag="IWK70_002236"
     CDS             complement(10156..12603)
                     /locus_tag="IWK70_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9614746.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12590..12979)
                     /locus_tag="IWK70_002237"
     CDS             complement(12590..12979)
                     /locus_tag="IWK70_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9614747.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(13039..13542)
                     /locus_tag="IWK70_002238"
     CDS             complement(13039..13542)
                     /locus_tag="IWK70_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9614748.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13555..>13731)
                     /locus_tag="IWK70_002239"
     CDS             complement(13555..>13731)
                     /locus_tag="IWK70_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614749.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4221 a         2710 c         2509 g         4291 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
      301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
      361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
      421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
      481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
      541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
      601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
      661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
      721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
      781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
      841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
      901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
      961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     1021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     1081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     1141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     1201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     1261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     1321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     1381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     1441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     1501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     1561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     1621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     1681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     1741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     1801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     1861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     1921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     1981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     2041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     2101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     2161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     2221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     2281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     2341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     2401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     2461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     2521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     2581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     2641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     2701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     2761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     2821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     2881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     2941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     3001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     3061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     3121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     3181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     3241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     3301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     3361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     3421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     3481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     3541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     3601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     3661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     3721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     3781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     3841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     3901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     3961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     4021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     4081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     4141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     4201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     4261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     4321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     4381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     4441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     4501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     4561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     4621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     4681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     4741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     4801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     4861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     4921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     4981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     5041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     5101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     5161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     5221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     5281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     5341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     5401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     5461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     5521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     5581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     5641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     5701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     5761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     5821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     5881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     5941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     6001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     6061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     6121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     6181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     6241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     6301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     6361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     6421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     6481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     6541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     6601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     6661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     6721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     6781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     6841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     6901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     6961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
     7021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
     7081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
     7141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
     7201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
     7261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
     7321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
     7381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
     7441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
     7501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
     7561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
     7621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
     7681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
     7741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
     7801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
     7861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
     7921 accatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
     7981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
     8041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
     8101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
     8161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
     8221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
     8281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
     8341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
     8401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
     8461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
     8521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
     8581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
     8641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
     8701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
     8761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
     8821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
     8881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
     8941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
     9001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
     9061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
     9121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
     9181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
     9241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
     9301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
     9361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
     9421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
     9481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
     9541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
     9601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
     9661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
     9721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
     9781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
     9841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
     9901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
     9961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    10021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    10081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    10141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    10201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    10261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    10321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    10381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    10441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    10501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    10561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    10621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    10681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    10741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    10801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    10861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    10921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    10981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    11041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    11101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    11161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    11221 ggtaactgaa tccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    11281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    11341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    11401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    11461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    11521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    11581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    11641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    11701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    11761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    11821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    11881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    11941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    12001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    12061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    12121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    12181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    12241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    12301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    12361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    12421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    12481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    12541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    12601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    12661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    12721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    12781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    12841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    12901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    12961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    13021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    13081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    13141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    13201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    13261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    13321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    13381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    13441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    13501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    13561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    13621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    13681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt g
//
LOCUS       DACDTO010000079        13516 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDTO010000079 DACDTO010000000
VERSION     DACDTO010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13516
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.079"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(334..894)
                     /locus_tag="IWK70_002240"
     CDS             complement(334..894)
                     /locus_tag="IWK70_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9614750.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            1036..1650
                     /locus_tag="IWK70_002241"
     CDS             1036..1650
                     /locus_tag="IWK70_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9614751.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            1839..3719
                     /locus_tag="IWK70_002242"
     CDS             1839..3719
                     /locus_tag="IWK70_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9614752.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            3798..4025
                     /locus_tag="IWK70_002243"
     CDS             3798..4025
                     /locus_tag="IWK70_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9614753.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            4030..4572
                     /locus_tag="IWK70_002244"
     CDS             4030..4572
                     /locus_tag="IWK70_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9614754.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            4799..5515
                     /locus_tag="IWK70_002245"
     CDS             4799..5515
                     /locus_tag="IWK70_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9614755.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(5575..7593)
                     /locus_tag="IWK70_002246"
     CDS             complement(5575..7593)
                     /locus_tag="IWK70_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9614756.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(7606..8604)
                     /locus_tag="IWK70_002247"
     CDS             complement(7606..8604)
                     /locus_tag="IWK70_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614757.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            8802..10043
                     /gene="sstT"
                     /locus_tag="IWK70_002248"
     CDS             8802..10043
                     /gene="sstT"
                     /locus_tag="IWK70_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9614758.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            10190..10825
                     /locus_tag="IWK70_002249"
     CDS             10190..10825
                     /locus_tag="IWK70_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9614759.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            11016..11756
                     /gene="truA"
                     /locus_tag="IWK70_002250"
     CDS             11016..11756
                     /gene="truA"
                     /locus_tag="IWK70_002250"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9614760.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(11901..13499)
                     /locus_tag="IWK70_002251"
     CDS             complement(11901..13499)
                     /locus_tag="IWK70_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9614761.1"
                     /translation="MLFLSFAYVIPLFMSKGIYHSVNQDTYFHLSRIIGLDNVWSSPV
                     NFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFITFLTMVVSYFSMK
                     QIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLILMGCYEIYIRDYQK
                     WYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLLSLLKATVLTFFLS
                     AGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYTIGLFLFLGLIGAF
                     IFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVWRLNGFSSLFIAYT
                     MSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLTLIAPEDAVAIAEN
                     YNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIELDNSNNKNTVLTTP
                     IYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTKLAIASRYLSIVTL
                     ILFLLYRFTFSKQKQPRREVQHSH"
BASE COUNT         4531 a         2135 c         2631 g         4219 t
ORIGIN      
        1 tgattcccct tgaaaatgaa ttgaggtaaa cgagaagaat tcaataaact ggatgataaa
       61 aatataatat gactataaat tggttacagc ttcaacctat aagttgtaat aaattagcct
      121 aaaagtaatt tattaatgat gaggtgaatt tgattggtac aacaagatat atcacttaga
      181 gagggagaag cagcactaca aactactttt caataagtag gaattataga aattttactc
      241 aaaaaatctt cagtaaacaa tttggctaaa taagattcag ttgttatacg agaaggttac
      301 ggttcttctt aactaagctt agtatgtttt tattcaaggc atgattttct ctatgtgatc
      361 attcagtttc tgtaagagtt ctaataattc ttttttggag tacttgtaat tgctttctac
      421 ccaccgattg agaacggata gacaaccttg gacgtggtac gtagtaaaga atctcatttc
      481 ttctgtgata atcgttttct tgtcttcata tagccatatg ttcagatagt tttcttcaat
      541 aacttgttta actttcttct gaaacgaaat atcaccatgg ttgaggataa ataagcgata
      601 taaaaaagta tttgcgtaaa catagtcaat caagcgagtg tatagttcct catagctatg
      661 ggctggatca taagagagaa tttcatttag atcccgaaaa aagttgcttt ctaattgatc
      721 atatagatca tagatatcct tataatgtgt ataaaaagtc gaacgatgta catctgccgt
      781 ttctgttagt tcgcgtatag tgattgcttg aatcttttta tttactaata atgtggatag
      841 tgtttcagcc aaagcttttt ctgttttttt gacgcgtcta tctgtctttt tcatttacct
      901 atctccttat ccgacatatc aaagcaaagc tgtcgcttaa tcgacaaact tatagatgat
      961 tgattataga tagtagacac tgtgtctatt ataatgtaag tataacacaa tcattgaagt
     1021 tgaggaggcg aaatgatgaa actaactttt aaacccttag aaaagaaaga ttacaaaaaa
     1081 gctatctatt ttgctgttga aggaatgaac tttgagagat atatggaaaa tgaacttctg
     1141 ctgaatcttt acggaagata ttttttgtat atggagttga atcgagcaac tcaaattctt
     1201 gctttgtatg cagaggacga gttggcaggt gttttgctgg cggagataaa aggagaaaaa
     1261 aagaaacatc gttcttttgg acagcaaata tatgttaaga tattcgattt tttacaaaat
     1321 atatttgcta aagaaagtaa aggagcttac gagacagcaa atgaacaaat gcttgcagca
     1381 tatttaaaga agcattcgcc agatggagag atcatttttt tagctgcaaa tcctgatttg
     1441 aaaacaaaag gcatcggtac caagttgcta acagaatttg aaaaaagaga gaaaggaaaa
     1501 gaaatattcc tttttacaga tagtggctgt acgtatcaat tctatgaacg acgtggattt
     1561 gaaagactaa aggaaaaaat tagcaagata aaaatcttag ataaagaaat agatctccga
     1621 tgtatgctgt acaggaaaat cattcaatga aaatttcgat agaaacgaca atagttggca
     1681 agatcacttt tagtgagtct tcttgctaac tattttttgt agtgtattct accaaaagaa
     1741 aaccgccttt ttgactaaac ttgatagtgg tcaatttttt atgatggata agtatctata
     1801 cttagtctcg taagaatgaa aggaaaagag ggagaaagat gaaattacaa aagtggatcc
     1861 aagagcatcg aaatcatatc accgccttat cagctgtgtt gattattttt ggttttatcg
     1921 gaaaatttac tggttttgtc ttgttctata acattgctct agccatagct tctattattg
     1981 cggtcatacc tattatgatc catgcgtatc aggcagtgaa agtcaaagtt atctcaattg
     2041 atttactcgt tactattgct gtgcttggtg cctttatgat cggagaatac aaggagtcag
     2101 ctatcgttac attcttgttt ttgtttggta gctatctgga acaaaaaaca ttgaccaaaa
     2161 cgcgggagtc aatcaaggct ttgaccgaga tggccccatc tactgctagt gttgtccaag
     2221 aagatggaac gactgaagaa gtggaaatcg atgacgtgga agaaggcgat atcgtcttag
     2281 taaaaactgg tggagctatt cctgtggacg gacgagtcat tgacgggaaa gggtatgcgg
     2341 atgaagccgc agtgactggt gaatcacgac aagtcgtcaa aatcagtgga gatgaagtct
     2401 tctctggaac gattttagca gatggctacg tgacgattga agcaactaag gtcggagatg
     2461 atacgacctt tgccaaaatt atcgaattag tagaagaagc acaagatacc aaatcccatg
     2521 cggaaaaatt cattgatcga ttttctcagt attatactcc aaccgtttta cttattgcgt
     2581 tggttgttgg tctgattagt cgtgattttc gtttagcaat cactgttttg gttcttggtt
     2641 gtcctggcgc tttagtcatt ggtgcgcctg tttcgaatgt agcaggaatc ggtaatggag
     2701 cgaaacgggg catcttagtc aaaggcggtg aagcaatgga tactttttcc aaaatcgata
     2761 cttttgtctt tgataaaact ggtacattga ccaaaggaac tactgctgta tccactatca
     2821 agaactatac agaggatgaa ggggacagct tagctttagt ggctcgatta gaaacgttat
     2881 ccgatcatcc tctggggcgc gccgtgatcc attatgctca ggagaaagac attgattttc
     2941 aacaattagc agtcaaagat aatcagacaa tcaagggaca aggaatcaca gctgagattg
     3001 atggtcatac agtttgtgcg ggcaacacca aattgattgc cgctcacaaa ctttctctca
     3061 caccaaagca agtacaagat ttacatcaat tacaacaaac aggcagctcg gtcattattg
     3121 tagcaatcga caaaaaaatc actcaaatca taggtgtgtc agatgtgatt cgaccagaag
     3181 tcgctgaaca attagctaag ttgaaacaag caggagcaaa acagttagtg atgctgacag
     3241 gtgataatca gatgactgct gattatgtag ctgatatgtt aggcattgat gaagtccatg
     3301 ctgaattatt accagacgaa aaggttcaat ttgtgaaaaa ataccaagaa caaggattgc
     3361 gcgttgcttt tattggagat ggaatcaatg attctccctc tttggctgcg gctgatatcg
     3421 ggattgctat gggatcagga acggatgttg ctatcgaaac gagtgacgtt gtcttgatgc
     3481 aatctaattt tgaatcgttg gttcatgcct accgcttggc taagaaaact gttttaaata
     3541 cccgtgaaaa tgtagtgatt gccattggtg tcgtcttatt tttactcaac ggcttatttg
     3601 ccgggtttat ttacatggct agtgggatgt tcgttcacga agctagtatc ttgatcgtta
     3661 tctttaacgc gatgcgttta ttgtattttg gacacgaaag caaagaagaa aaatcgtaaa
     3721 acttgataag aatcaatttt tttatggaag ctgtaatcta gaataagact atcaagaaaa
     3781 aggaaggaag aactacgatg aaaaaaatta ttatgcaatt agatacacta gcttgtccaa
     3841 gctgtatgca aaaaatcgaa catgcggttt ctcaacagcc tggggtaagc aatacgaaag
     3901 ttttgtttaa tgcgagcaaa atcaaagcaa actttgatcc agatgaagtc caccctgaac
     3961 aattagctgc agttgtggaa aaattaggtt atgaagtcca atcagcaaaa gtaaaagagg
     4021 cgtaaatcaa tgaatatagt ggaagaaaaa tttgttcaag aaacaaaaca agcagatatc
     4081 gaccatcata aaccaactgc tggcgccatg gtggggcatg tattagcaaa ttttaaatta
     4141 gaagaaatga aattgatcca agcacaattg tatgttttgg gtacatcttc gcaagaatta
     4201 agtacccttt tttcagaata tgctgatcaa gaagcgagat ggttcaataa aatctctcat
     4261 gagttagtga ttgaaaatga aatcgttccg tctactttag aagaagtcat gcgttatgga
     4321 aaaatttacg aacatggtca aaacaaatat ttaccagcct caacaatgtt ggaaaacttt
     4381 gcaaaagatt ttgatttcca aaatgtcttt atcacacggg caatcaaatt agctgaaaaa
     4441 gaagaaaaat atggcttgca acaattgctt gttgaacttt taagtttcaa taaagaaatc
     4501 atctaccaag tacaaagact ccaaggaaaa actgtccgac aagatttgga tgaggaagac
     4561 gatgatgact aaaagtctat gtttcttcaa atacaaatat aaagcgtgag gtaaattgga
     4621 aaatcacgaa gattaaaata aaagaagcac ccgctgatgt attacagaca acatcagcgg
     4681 gtgcttctca gtttttagaa gatttgaaag attgatgaga aaatacgaaa aatcaataaa
     4741 atcacattgc ttatttgata gaataaagaa gtgggagtaa tcgaaaggag ttttttaatt
     4801 gaaaaaaagg attttagtaa caattggtct agcttttttg ttagctggtt gtaccaacgg
     4861 gaatgaatcg aaaaaagaat caacaaaaga aacagcgaaa gcaacagttg caactactac
     4921 aacaactagc acagagagca agtcaactgc agcaagtaca gaaacagcag atacctctac
     4981 ttcgtcagag tcttccaaaa cgactgaaac aactcaaaca tctacttcaa gttcttctgt
     5041 agaggcttca tcttcctctt cagaaagcag cgaacaagtt gaacaaacac tgtggaatgc
     5101 agacaaatcc gcacaattag aggcatttat gaccaaatgg ggccaaacaa tgggacaaca
     5161 atacaaaagt tatacgaatc aaatgagtgt ggacttatat ggtcttaaag tccctcaagc
     5221 tattttgaac ggggagtgga agatggctat tggtggagtg cctgtttctg cagaatggtc
     5281 agaaagtggg acaggtcaag cagactatca aatcacagcc gtctattccg atgcagagac
     5341 agaaccatat ctgaaaaagc acgtctatct attcgggttt cagcaaaatc agccaaaagt
     5401 attggtgaca caacaaaatc aggggaatcc cgataattat ctttacttca acgagacagc
     5461 gaataatgaa ttgaaaaatg gctttaatca aatcgtttat ggacaagcaa attaatgaga
     5521 taaacaaggg gttgtgacat gtcttttatc acaacccctt gctcagttta tttgttagtc
     5581 ttttattttt tgtgtcgccc atgttgcact gtactggtta cctgcaacaa gatcatattg
     5641 tatcagacga taatcttcta gaatttcttt ttgtttctca gttaaatttt tttcttggat
     5701 aatttgacta tcctctgtaa caaagacctg ttttccatta aaacgattag acaagctttc
     5761 tgtaggatca attgttaatg caggtagatg attagatact tctgtcaaca acgcatagta
     5821 aggagttact ttgatattcg catgctcaag agccatagca ccaaagttat taggagcaac
     5881 aacttcttta tttaacgtag ctgcttgttc acgactatac ttgttgttat aaatgaaata
     5941 atctgtctgg tgctgttcta atccatattt tttcgaatca ttcccactat agattgcggg
     6001 taaatggtca ccataaaaaa caaaggtaat cggtttatcg attttgtcga tttcttggat
     6061 gaattttttt actgctgtat cggtatagtg aaggccttgc atataagttt gtaattcttt
     6121 tcttctatta ggaatgacag cagttccttc agcagtgtag ttcaattcac tatagtaatc
     6181 atcataaggc atatggtttt gcatagtgga taattggatg aattgtgacg tttccgtatt
     6241 gctttgtaaa gctttcaagg tttctttgta tgctgaatca tccgatatgc gtgggctttg
     6301 ttctatttta tccgtataag ttaatttatc cggactgtct aaataataga acctgtcaaa
     6361 accaaattta tcaaacactt gttttctgcg ataaagagca gctgtaaaag gatggatggc
     6421 tattttttca tcgaatagat cggtaatatt tggacttatt ttctgacgct ccactagctg
     6481 ggtataaggt gtaactaacg aagcagacag gttactgata ctcaagcctg tcagtccttc
     6541 ccattccatg ttagctgttc cgccaccata accgacggat aacatcaatc ctcctgttcc
     6601 ttctgttata atgcttttga tgtaaggaat agggtctgta ggtactgtca gattagggac
     6661 acgtgcagga tcactgaagc tttcacttaa attgagaacg atcgtctgat tgtttaacca
     6721 gtcacttctt gtttggttga tttcatctgc ctttgtgttg tactttttca ttattccatt
     6781 aattttttct ttcgaatagc cagcaggttc ttccatgatt ttaatgtcga tattgtttag
     6841 gaattgaatg actggtccgt tatcttttac ggcgccttgc tggttaaaga aagtcttgtt
     6901 tactttaaat aaattaaaca gcaagaaaga aggcgaattt ttgtgattaa tgaaaaagac
     6961 acccgaaaaa catcctaata atacagcgat actgataaat cgacgtttcc aatgaatctt
     7021 caaggaatac tgtttttgta gtctacgttg tacaataata ctagtaatca ataataggag
     7081 aaggatcaat ccgcccacga ttaataagac tgggctaacc atcgataata gttctgacaa
     7141 tccggtcagc atttttaaat ctgaaggaaa gacaggctct tcccgcattt ccattttgat
     7201 tactgtcgaa atagtgagca gtaagttaat gatcaaggta gcagtcaagg cgtaccaaaa
     7261 acgattggaa atcaacaata gtaagaagaa aaatcccata ataatgatga ttgtcaggat
     7321 catgggtgct tgtcgctgag taaatatttt taaggcaacc tggatagttt ctttccaacc
     7381 ttcttttggt tctaaaacga aaatctgcac gaaggtaaag cagtaaaaaa atgctgcaac
     7441 taaaaaataa cttttatgaa caaaaagcca atgaattctt tttttccaac gctgagttaa
     7501 ttgttcaatc gaatcaaaag cgaagtattc gatgaaacga tgaatgaaac ggattttttg
     7561 tatataaaat aaaaagataa ctaggaggga cactgctaat aaatatcaaa taaaaaactg
     7621 cccgatattt atcagccatg ccttccctga gtttggaaat tcacgtttca taaatgtaga
     7681 tacgcgataa gaaactaaag ggaaattaca tataacttga gtgacaatca aaaatacagc
     7741 aagaaaattt cctcttcctt ttatacctaa tttatcagat agcatttcta aaaataaaaa
     7801 cgaagagaaa gtataataca ttgcgaagag agaatatggt ttagaaaacc tcagcgctgt
     7861 atctgtattt cctctgatga tgagtgaaat ttgtgtcatc aaaataatca aaccgaataa
     7921 aacaatcaag ctgataaata cttgaggaag cttatagcgg accttttctt tccagttcca
     7981 ttctttcaaa ccataaccca ttaaaaataa ataaaaaatc cataaaatac tctgtccatt
     8041 ctgtaatgca aacgggtctt tattaaatag agtagagcag ccaactaatc caattgttaa
     8101 aaataatagt agatatttaa ttgttgtttt cgataattta tttaaaaatc caactgtata
     8161 aggaatacta caaaaaagca aagtacaaga aacagcataa gtaaagtaat tctgactgat
     8221 aggtgttagg ataagccaat aatcacgaat attcaagctg ccagcttgta cgtaaaaaaa
     8281 gatattgcaa actagtgtgg ctattaggaa tataaaggca aaacgactta aataatgtaa
     8341 aacttttaaa cgggcgattt tttgatattg gtaacccatg taaagtggga tcaaatttgc
     8401 accaataaca acggaagttt gtaaaaatgt atagatgtag aatcgaccag ggtttgttgc
     8461 atatgtagga atagtgactg ctgaaatcaa tatacgctgt aaaaccatca ttatacaagc
     8521 gacaaagatt gtcaggaagc cataactctc cttctttatt tttttcaaga ataaacacct
     8581 cacaaaaaaa ttggacaaaa acataagaaa acgtttcctg ataagttgcc cattaataac
     8641 atcctaactt agaatatctc aaaaaacaac gtgtagacag aaaaagtaca aaattagcaa
     8701 gaaatggttg tttgtattgt tagaattttt agaaaattat acctttagtt tctttgcata
     8761 aaatgataca atgattgtta aaattgtgaa agggtggtca tttgttagag aagtttttgg
     8821 atatgtcgtt agttcaacgt attgttgttg gtattttagt cggaatattg tttggtatct
     8881 ttttccccac atggacattt atttcaatat tgggaacgtt gtttgtcagt tgcttgaagg
     8941 caatcgcacc tattctggtt tttttcttga cgatggcgtc aattgccaag cataaaattg
     9001 ggaataagac attcgtgaag cctattttga tcttatattt agtgggaaca ttgctttctg
     9061 cacttgtagc agttattgta agttcaattt ttactgttcc tattacttta caagaaacag
     9121 tatctgaaaa agcaccgcaa agtttggaaa gtgttttatc tactatgttg acgaatgtca
     9181 cacaaaatcc tgttcaatct cttattgatg cgaattacct tggcgtctta ttctgggctg
     9241 tattgttggg actagctttt cgagcacgtt cagaatcgac aaaagagttg gtggatcaga
     9301 tatctatagc gttgtcacaa gtcgtacaag tgattattgc atttgctccg atagggattt
     9361 taggattggt ttatcaatca attgctacga ctggaatcgc tggattggca gagtatctgc
     9421 agcttttact tgttttgatc ggtacgatgc tgtttgtcgc attagttgtg tacccattcc
     9481 tcacgttttt gtttattaaa gaaaatccct atccattgat tttcttttgt ttgaaagaaa
     9541 gcgcacttcc cgctttcttt acccgaagtt ctgccgcaaa tatcccgata aacatgatgt
     9601 tggctgaaag attgaaattg acaaaagagt cttattccat atcgatcccg ttaggtgcta
     9661 cgatcaatat gggcggtgcc gcgattacca tttccttgat gacgttgact gctgtacatt
     9721 ctttagggat tgaagcacct tttgttttga aagtcctttt atgtattttg tctgctttag
     9781 ctgcttgtgg agcttcaggt gttgcgggcg gttcactgtt attgattcca ttagcatgta
     9841 gtttgtttgg tatctctaat gatgtcgcga tgcaaatcgt cggtattgga tttattatcg
     9901 gtgttatcca ggattcaatg gaaactgcct taaattcgtc aagtgatctg ttgtttactg
     9961 caataggaga gctaggcgcg cgtaaaagaa gtggggaaaa aatcaaattg aaagattgtt
    10021 tagatggatt aagtgaacgt tgaacgttag atatttagta tttttaaaat gattcgagtt
    10081 tttaatgagt tatatagatg ttcgtgtaaa tagaggtttc tgttaaatca agataaagtc
    10141 gtttataata acaattaaaa gcataggtaa ttgttattgg aggcgtatca tggatttttt
    10201 acgtaaattt ggttttttct ttttattatt aggcgtgctg agtgattttt tgactccgta
    10261 cgtattagga ttgtattatc ctgaattaga tcaaatgaag agcgtgatca gtgtatttgg
    10321 agaggtttcc agtcctgtaa gaaaagcgtt tcttatttgg tctgtggttt cagggatctt
    10381 tttcatcctg tcattgcctg cattgtatgc tgcatttgtt agtacatctc gtgtgttagc
    10441 taccctagtt acagcagcca tcgcgttcta tggtataggt gattgtatct ttacaggttt
    10501 gttcagtatc aatacccatg aatcttcgtg gaatgtctcg acatgggtcc ataatatcgg
    10561 atcaggttta ggttacagtg gttttttatt gtttccagtt cttcttttct tgatctatcg
    10621 aaaaaatggg caaatggacg taagccactt ataccttgga ctgtctgtga tcagtttgct
    10681 ttttgcggcg atttatggac tggcaagaat tccttctata aattcttggc cattactgaa
    10741 taaaataggt ttttgccaaa gaatcagttt tttctttaat tatgtgccca ttgccaggtt
    10801 gagtatcttg catctcaaac agtaaaactt gagccgtcaa attgaacagt cagcggatac
    10861 ttcatccggt ggctgttttt tgtttttctg aaatatatat ttccacagtt ttcttgtata
    10921 gcttctgtct tagagaagca aggagctata ttttgtgcta caatcttaaa aaggtagaga
    10981 gaagaccaaa tggaagacag aggagccact aaaatatgag aaacatcaag ttgacaatcg
    11041 aatatgacgg aaaaagatat tcaggatggc aaagacttgg agatgacgat aaaaccattc
    11101 aaggaaaaat cgaaaaagta ttgcatcaga tgacaaatga agagatagaa attattggat
    11161 caggaagaac agatgctggc acccatgcgc gaggacaagt agcaaatttt aaaacaaaca
    11221 cagaaattgg actggcagaa atgatcgact ttatgaatcg ttaccttccg cgggatattg
    11281 tcatcaaaag aatcgaagaa atgcctgagc gcttccattc aagatataat gcggtgggca
    11341 aaaaatacag ctattatgtt tggaacaacg tgattccttc agcatttgag cgaaaccaca
    11401 gcttttattt ccctcaagag ttggatatgg acaagctgaa tgcagcttgc gaaaaattag
    11461 tgggtacaca tgatttcatt ggtttttctg cattgaaaaa atcaaaaaaa tccacagtgc
    11521 gtacgattga tgaattatct atcgaacgaa agggagaaat gcttcatttt acttttgtcg
    11581 gagacggatt cttatataaa atggtgcgga ttatcatggg gacattacta gaaatcggca
    11641 caggtgccat gccgatagaa acaatcgatg aaatatttga aagcaaagtg cggcaacatg
    11701 caggagaaac cgtccctgca caaggtctgt ttttggatga agtttattat cgataaaaat
    11761 actttgataa tggatgaaac gcatgtttca cagtataaat atcgatgtta aataatgtat
    11821 gaagcaaatt aaagaaatat aaaaaaagac tttttagcct gtttatgtgt acagctaaaa
    11881 agtctttctt aaataaaaat ctagtgagaa tgttgtactt ctcttctcgg ttgtttttgt
    11941 ttagaaaaag taaatcgata aagtaaaaat aaaatcaaag taacgataga taaatatctt
    12001 gaagcaattg ctagttttgt gtactgataa gtaataacga ctttatttat acctggagga
    12061 atagttaatt cagttgtgcc aaacttagat aattttgttt gtactagttt gccattaata
    12121 gaagccactt gccctttata acgataaata ggtgtcgtca agacagtatt tttattattc
    12181 gaattatcta attcaattgt aagtttgtta ctcataaaat gagttgttgg atttatttcc
    12241 tggttaccta gtaaatattg atcattatta atcatatcag gatgatagat tgactctttg
    12301 tttgcataat cagtatgatt gtaattttca gctatagcaa cagcgtcttc aggtgctata
    12361 agtgttaatg aatctttgtt agcatggata gaattgctta ctcctgagag atgcaatatt
    12421 agtgcaagaa acgtaaagac cattattttc catgaattat ttttagtaga aaaaatataa
    12481 tagatcacta tactcatcgt ataggctata aataaagaag aaaaaccatt taaccgccag
    12541 acaaattgta gactcttaac tggtgtatgg ttaaaaagtt gccaaggaaa taaattggtt
    12601 gaacaaaaaa gaacaaatac accaagaaag aaaataaata aatcatcagc agttaatttt
    12661 tttataaaaa taaaagcacc aatcaatccg agaaataaga ataatccaat agtatagttg
    12721 ttatagttat tatttaaaat atgtgtcagc atatcactag gagccatacc acttaattct
    12781 tttcccagtg ggactttcaa ttcttgtgct ctagattgtt gaataaaagg aattaagaat
    12841 cccgcactta aaaaaaaggt gaggacggta gcttttaaaa gacttaagag tctagcgatt
    12901 ttttggtccc aaaaataaaa agaaagaaac aacgttcctc caataaatac agaaaccata
    12961 gcgacagaaa gtaaatgagt atacactact agcgtcatgc caatagacaa ccagtaccat
    13021 ttttggtaat ctctaatata aatttcgtaa caccccatta gtatcaaagg caaaaatgtt
    13081 aaagcgacag cttctcccaa tgaagcacga tggaatatgt caatggcacg ataggcggaa
    13141 aatgtataaa taatcgaaaa taacagcgaa atataacgat tgttttttat ttgtttcata
    13201 gaaaaataag aaacgaccat tgttaaaaaa gtgataaaaa aatagtagat attataactt
    13261 aaaacaagat ttcccatcat tttataaaat aaaaaagctg gataaagagt aagccatgga
    13321 taaaaaatat tcatcattgt tccatgatga tcgaagttat tgaagtttac tggagaactc
    13381 catacattat ctagtcctat gattcgagat aaatgaaagt aagtatcctg attgacacta
    13441 tgatagattc ccttagacat gaatagaggg atgacgtaag caaaacttag aaataacata
    13501 aaaatgatat ctatta
//
LOCUS       DACDTO010000080        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDTO010000080 DACDTO010000000
VERSION     DACDTO010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.080"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWK70_002252"
     CDS             complement(<1..914)
                     /locus_tag="IWK70_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9614762.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWK70_002253"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWK70_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9614763.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWK70_002254"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWK70_002254"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9614764.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWK70_002255"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWK70_002255"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9614765.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWK70_002256"
     CDS             4440..5321
                     /locus_tag="IWK70_002256"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9614766.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWK70_002257"
     CDS             5318..6034
                     /locus_tag="IWK70_002257"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9614767.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWK70_002258"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWK70_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9614768.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWK70_002259"
     CDS             6751..7752
                     /locus_tag="IWK70_002259"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9614769.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWK70_002260"
     CDS             7749..9110
                     /locus_tag="IWK70_002260"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9614770.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWK70_002261"
     CDS             9070..10221
                     /locus_tag="IWK70_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9614771.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWK70_002262"
     CDS             10196..11365
                     /locus_tag="IWK70_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9614772.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWK70_002263"
     CDS             11540..12919
                     /locus_tag="IWK70_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9614773.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWK70_002264"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWK70_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDTO010000081        13398 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDTO010000081 DACDTO010000000
VERSION     DACDTO010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.081"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(14..706)
                     /locus_tag="IWK70_002265"
     CDS             complement(14..706)
                     /locus_tag="IWK70_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9614774.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            791..967
                     /locus_tag="IWK70_002266"
                     /pseudo
     CDS             791..967
                     /locus_tag="IWK70_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1143..1712)
                     /locus_tag="IWK70_002267"
     CDS             complement(1143..1712)
                     /locus_tag="IWK70_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9614775.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1786..2109)
                     /locus_tag="IWK70_002268"
     CDS             complement(1786..2109)
                     /locus_tag="IWK70_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614776.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2263..3075)
                     /locus_tag="IWK70_002269"
     CDS             complement(2263..3075)
                     /locus_tag="IWK70_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614777.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3429..4277
                     /locus_tag="IWK70_002270"
     CDS             3429..4277
                     /locus_tag="IWK70_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9614778.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4422..5363
                     /locus_tag="IWK70_002271"
     CDS             4422..5363
                     /locus_tag="IWK70_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9614779.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5353..7524
                     /locus_tag="IWK70_002272"
                     /pseudo
     CDS             5353..7524
                     /locus_tag="IWK70_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IWK70_002273"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IWK70_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9614780.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9827..12013)
                     /locus_tag="IWK70_002274"
     CDS             complement(9827..12013)
                     /locus_tag="IWK70_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9614781.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IWK70_002275"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IWK70_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9614782.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IWK70_002276"
     CDS             complement(12235..12675)
                     /locus_tag="IWK70_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9614783.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IWK70_002277"
     CDS             complement(12749..13288)
                     /locus_tag="IWK70_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9614784.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2380 g         4446 t
ORIGIN      
        1 agggggggat tttttatcgt tgtggaatat ttaaatacca acgcttgtca tgaaaatctt
       61 gtgctccacc cttagtattt ccttctggat cgtttgtcgc acgcatcatg acgtatactt
      121 tcttattagg gaaatcacgc atattgaaag atacatgata gccaacattt cctaaagtat
      181 tataagcttg atttacgtct ggtctataaa tcccatcagc tcttactcta gctagttctt
      241 ttccagtatt gtagtccata atgaaaatat actcgtattt ataattagca atgtgccatc
      301 cagctacatg caagtttgcg ttttcgattt ctccgaactg atcaatgtgg gcgtaatttg
      361 ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt tggatcaatt actggtttat
      421 cctccgaagt agttggatta tcatccgtaa aaccatgagc caaatcatag gctagttttt
      481 ctttacttac acccatttca gaaagataac cgtaaggatc tgtatgatcg ccccaaatat
      541 tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggagtg tctaatgtta
      601 atggaatacc atatttcatt gctgaatctc ttgccaattc aaagtatact ttatagttct
      661 tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat ttgcacagga cttttggctc
      721 acgaggttgt cctactctac atcttgctga tagatatgct gaaaggaaaa caccaacatt
      781 atgttttgct ttttcagcta gaacgagaca agcaggaata cctttagaag atatcaaagg
      841 ttttttaggt cataaagatg tttccactac acaagtctat gcccatattt ctcccgaagt
      901 gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa gagcaaataa aaaagcactc
      961 aaattgagtg cttttttaag ctcactatca ctaaaactat cactaatttt atttataagc
     1021 gaacatgttt ctatcactga ttttttaaat ctagcgaaaa acccttatct taaaggcgtt
     1081 cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc tagaagtgcg aacatggttt
     1141 tcttatagct catgtagaaa aattttcttg atgatgattt tatccatcaa ttgagattga
     1201 ctgttttgct ctcctatgat ttcagctatg attgtccgat tttgcggttt tcttcttaac
     1261 tggtagttga ttttcccagt ttccatcaac tcgctaagca tatccacgac tccgccaaat
     1321 ccggtctttt tcgtaataaa acctagtgct accattcgtt catctagatt ttttgctgta
     1381 atcgtcacac cagtaatgag cgagtgctgt aatgtttggg ccagctcttc catcccttca
     1441 gcattggata cgacggaagc tgttttgtta ataggttctt cgtttatttc ttttggttct
     1501 ttcctctcca ctttcactgt ttgacgaagg acatctagtt cttgcaagag cgtttcgtat
     1561 tgttcttggg tcagcatcac gccagctact ttttcacgat taaatatata tacgccagtt
     1621 cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc gtttcacttc tgtgatcgaa
     1681 gatgtgggaa cttctaattt ctttaattcc aatggatttc ctcctttact aatcgctttt
     1741 tattatagca cagactgact tgttttgatc ttttttccaa aggttttatc ggtttttgcc
     1801 taaccagtaa ttgatatcgg taagaagtct ttcgatacca aatgaaggat ggtacagata
     1861 cgttttttga tcttcgttat ggtctagtgg aaaaaaatga tcgatgatca ctagatctgc
     1921 ataccgaggc ggcttatttc gagtacatac atgtatagag atccgttttc gatgtcttag
     1981 cgttttgttc agtaccccag caagtcttct tgcttcttgg tgttcatagc gaaaaaaaaa
     2041 gacgattttc acttcaggag ggtggacatc taaataccct cgttcctgga gtgttttcag
     2101 taaaagcatc ccccactcta ctgcggggtg tgcaagtgct tgttggatcg ctagccgaaa
     2161 ttgatatagt ttatctttgg aattgggaaa tttttctaat gtttcctgat aataacgaac
     2221 ttcttctatc agccggtcat ggataccaat tacgtaacga agtcattctt ggtaaagtac
     2281 ttttgtcagc tttgatttgc tttcgctctc aaattcgcta ttcagccaaa acagcgaatc
     2341 aagcttttca gtcacaatcg tcaatttccg atagtcttgt gtctcttcta tccgtttcca
     2401 ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt gctagataat ttttttcttt
     2461 tattggcaaa atctgctcct ctctaaatag caattcagga aacaattcaa agaatagtgt
     2521 tcgactgtct cctaaaatct cgatttctgc cggatccttt ctttgcttca ataattggat
     2581 gaacatctca gaaaaggact gctctccatc atctgcaaaa aaatgcggaa aatctttcaa
     2641 aaagcaaaac agcagatatc ttacttttct ttctgaacca aagatctgtg cttttttata
     2701 agaaaaagaa aggtggtact cgccaaacca ctttctcaat cgctcttgcg tagaacgatc
     2761 gtacttctgc tcgtaaggtt cattcaatag cagatttttc agtaaacaaa acgggggtga
     2821 ttggatcaga aaaacaaaca agagataagg ttcgctatat ccttgatcat aaactaactg
     2881 aacggttttc ttatgaagaa tcagctgaat gggacagtcg ctttccgcca aacgtttcag
     2941 atgccgatcc actgttttaa catcgatttt cagttcttca gctaaatcag cttttcgcca
     3001 gatcaaatct ttcattagat tgattagtga aaataaattc tgtgcatttt ttgataatag
     3061 tttttgccat ctcatcgatc atttctcacc tgatttctaa acatattgtt tgatcaaaat
     3121 gggttcactc ctaaaaagat aaaaaactcc ctttctattt atttaaatac gcaaatcttc
     3181 ctcattattt tttattttac tcaatttaat cgtattctca tttcaaaacg aatgtacttt
     3241 aaaaaaacat aataagatca ctttgcttgt atgatgtatt cccttccatt acaatgaaat
     3301 aaatagcgat tttatgagaa ggtgatgtga aaatggaaga aaaaatagaa gaatccaaac
     3361 aaaaaatcga tcagttgata taagaattag aaaaagcggt catcagcaaa cgttcaacta
     3421 tccaattgat gatcgtggcc ctattagcag gtggtcatgt cttattcgaa gatattcccg
     3481 gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc tgtacaggga accttcagca
     3541 gagtccaatg tactcctgat ctgctgccta gtgatattct tggtttttcc gtatacaatt
     3601 cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt taccacgatc ttgttagcag
     3661 atgagatcaa tcgtacgact cctcgtactc agtcagcact tttagaagca atggccgaga
     3721 atcatgcaac gatcgacaac aatacatacc ctctagacaa tcattttttt gtcttagcta
     3781 cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc ggaagcacag ctcgatcgtt
     3841 ttttattccg actacaaatc ggttatcctt cttttgatga cgaacttttg ctacttttgg
     3901 ataaattcga aaaagtgttg gataacttaa atgaagtatt gaacagttat gaaatagaag
     3961 aaataaaaac aaatgtggcg aatgtctttg tcagtcccga agtggcatct tatgccttgc
     4021 agttagtgtc agcaacaaga agtcatgctg ccatccagct tggcatcagt ccacgaggaa
     4081 gcctttcctt tattcaggca gcaaaagctt atgctttaat ccatgggaga aattacgtca
     4141 cgcccaaaga tttgcaggat ttagttcctt acgtgttttc ccatcgcctg attttttacg
     4201 atcgttcatt gaaggaagac gaaaagaaac aaatcattca tactcttgtt gcacaaattc
     4261 ctattcctgt gaggtagatt gaggtagaca atggaaaaaa tcaaagatta tttcattaat
     4321 tttgtgttat ttctggtata tttcatcatt gtgctttttg ctcttgtatt caacaattct
     4381 ttaggctggt tcttgttggc cttttttact ctttatgtat catggagctt gatttcatta
     4441 agcagctttc taaagaaact ttcggttact tgtacattgc agccactcgt ttatcgcaaa
     4501 aaaagtatgc aggttatttt ttccatagca aaaaagagaa ctttttcttt acctctccct
     4561 cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa acaagagatt ttgattctta
     4621 caaataaacc gcaagaagtc caattgcttt ggcagccaaa agaaagagga tgccatgaaa
     4681 cattaactgt catttttaca gcctatgata gtctaggact tttcagaaaa agtgcaaaaa
     4741 gagagatctc ttttcctgtg actgttctgc ctgctttttg taagattcaa gcagaaaaat
     4801 tacagagagt tctggaaaaa aaacagcagc tgaacgcgta ccaaagaggt cttgattttc
     4861 gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg aatcgattgg aaattgtctg
     4921 ctcatactca agagtggtta taccgtgaat acgattatca agaagaagaa tgttctcctc
     4981 ttttttgctt ttggggtttg cccagtccaa aatttgaacc tctgctagac ttgtattttt
     5041 ctttatggca tttgagaaaa gaaaagcagc cggaacttct gatattaggc aaccgcgcct
     5101 ttcatggcaa agaccccggt catacctatt ttgcttcttt ggaagctggc gatgagaaag
     5161 cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat cgtcctcttt gttcctgaga
     5221 attctcctga agtctcagaa gctattgaaa cattgtcaaa agatcgaacg gtttatcttg
     5281 tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga caagatatgt tcattgccag
     5341 gaggtgaatg gattgacgat taaacgggaa aaatggctgc gtggcgttta ctcctttgtg
     5401 atactaagtg taggtgctcc gatatttgta gtagctaatc aattgcaaaa cagctacctt
     5461 cttatcctaa ctattggtgt cctatgcctt attgtctgct gtattcctaa aaaatgggta
     5521 cgtttgctgc tttctttgcc agtaatgata ggttgtttgt ttttatattt tccttcgaag
     5581 acattttcca tattatggat cttacagttt ttttcgtcag tcacagagga attcttccat
     5641 ctatcccaag gtaaacttta ttatttgcca gaaaagacag ctttttttct cataatgttg
     5701 ctgatctatt tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagttttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaagc aactttaatt cgtaaagtcg aagctgtcta acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc aaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt catttttttg cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaatgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatc tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gcacagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttga cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct gattccagca cctgggtggg
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ccattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gcttactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga taataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt attttcggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggcac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaatgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacaat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagtcggtg atttctttga tcttccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcattc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataa
//
LOCUS       DACDTO010000082        13258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDTO010000082 DACDTO010000000
VERSION     DACDTO010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.082"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            122..1753
                     /locus_tag="IWK70_002278"
     CDS             122..1753
                     /locus_tag="IWK70_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9614785.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            1756..2835
                     /gene="uxuA"
                     /locus_tag="IWK70_002279"
     CDS             1756..2835
                     /gene="uxuA"
                     /locus_tag="IWK70_002279"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9614786.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            2909..4336
                     /gene="uxaC"
                     /locus_tag="IWK70_002280"
     CDS             2909..4336
                     /gene="uxaC"
                     /locus_tag="IWK70_002280"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9614787.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            4420..5067
                     /locus_tag="IWK70_002281"
     CDS             4420..5067
                     /locus_tag="IWK70_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9614788.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            5196..6572
                     /locus_tag="IWK70_002282"
     CDS             5196..6572
                     /locus_tag="IWK70_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9614789.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            6583..7377
                     /locus_tag="IWK70_002283"
     CDS             6583..7377
                     /locus_tag="IWK70_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9614790.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            7390..9156
                     /locus_tag="IWK70_002284"
     CDS             7390..9156
                     /locus_tag="IWK70_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9614791.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            9184..10992
                     /gene="uidA"
                     /locus_tag="IWK70_002285"
     CDS             9184..10992
                     /gene="uidA"
                     /locus_tag="IWK70_002285"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9614792.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            11164..11565
                     /locus_tag="IWK70_002286"
     CDS             11164..11565
                     /locus_tag="IWK70_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9614793.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            11559..11804
                     /locus_tag="IWK70_002287"
     CDS             11559..11804
                     /locus_tag="IWK70_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9614794.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(12128..12349)
                     /locus_tag="IWK70_002288"
     CDS             complement(12128..12349)
                     /locus_tag="IWK70_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614795.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(12511..12583)
                     /locus_tag="IWK70_002289"
     tRNA            complement(12511..12583)
                     /locus_tag="IWK70_002289"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12548..12550),aa:Lys,seq:ctt)
     gene            12659..12888
                     /locus_tag="IWK70_002290"
                     /pseudo
     CDS             12659..12888
                     /locus_tag="IWK70_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            12932..>13258
                     /locus_tag="IWK70_002291"
     CDS             12932..>13258
                     /locus_tag="IWK70_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614796.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFY"
BASE COUNT         4755 a         1723 c         2620 g         4160 t
ORIGIN      
        1 acaataattc tgacatggta gaaaaaagaa tatttttagt tttttcttat tgacagcgat
       61 tacattcgat tttatactac aattgtaacg gaaactaaca tatcagtatg gaggggtaaa
      121 tatggttttg attaaagact ggcaagattc tatcgaagga ttgaacaaaa tgcaagttca
      181 actaccgtat tttaatgtcg aagcggtcaa acaagaaggt cagaaacatc cagtgtggct
      241 tcattttggt ggaggaaatc tttatcgagg atttcatgca gaaattgctc aatgcttagc
      301 agatcaaaat gaattagaat ccggagtagt tgtgtgtgag acatttgatg aagaagtagt
      361 agaaaaagct tatcatgact acaacaatga tattttggag attgttatgg gtgaagatgg
      421 tgaactcaaa aaaagacttt tagcagtaac agctgatagt ttatattgca atccaaagaa
      481 tataacggac tatcaaagag tggttaaata ttttgaagat cctacattac agtttacaac
      541 gtttactatt actgaaaaag gatacagttt aaaagattct aaaggacaat ttttttcggt
      601 agtggaagca gatataaaat ctggacctac caatcctcaa catacgatga gtattgtgac
      661 atccttgttg tatagcaggt ttaatgctgg agagttaccg attgcgatgg tttctactga
      721 taatttctca cagaatggtc aacgttttca agatagcatt tttatgattg cagaagcttg
      781 gcaaaaaaaa ggatacgttt ctgaaaaatt tattgcttat ttaaaggata aagaaaaagt
      841 gacttttcct tggagtatga ttgatagaat tacgccaaat ccttcggaaa gtgtcaaaat
      901 ggatttattg aaggcgggtt tcgaagactt agatataatt catactgcga aaaataccaa
      961 tatcgctgct tttaccaata ctgaaaaagt ccattatcta gtcattgaag atagttttcc
     1021 aaatggtcga cctaaactgg aagcggctgg tgtgattatg acagatagag aaacagttga
     1081 taaagcagat atcatgaagg ttacgacatg tttaaatccg ctccatacgg cattagcaat
     1141 aacaggttgt ttgttggggt ataaatcaat tcaggcagaa atgcaagatg atgatttggt
     1201 tggcttaatt aaagggatag gttatgtaga aggcttacca gtagttgaaa gtccaggcgt
     1261 tattgatcca aaacaattta ttgatgaagt aatcaataaa cgtttgccta atccctatat
     1321 tccagataca ccgcaacgaa ttgcaacaga tacttctcaa aaagtagcga ttcgctatgg
     1381 tgaaacaatt aaaaagtatg tcgaaaaaga cggaaatgca gaaaatctaa catttattcc
     1441 tatcgctatt gctgcttggt tacgttattt attggcagtc gatgatcaag gaaaaaattt
     1501 tgtgccaagt cctgatcctt tattggtaga gttgcaggaa aaattctcag tattgaaatt
     1561 aggtgaacaa gatcctgcaa agattcattg tgcggtacag gaaatcttag aaaataagga
     1621 aatatttggt ttagatttat atcaagttga gataggaaag aaaattgaaa aaatcttttg
     1681 cgaaatgcta gtaggtcctc aagcagttcg taaaacaatc aataaatata taacagaagt
     1741 tggaggaaag taactatgga aatgactttt agatggtatg gggcaaatga agaaaaaatc
     1801 tcactacaag aaattcgtca gatcccagca atgaaaggaa ttgttggaac attaatggat
     1861 ataccagccg gagaggtctg gccgagagaa cgaattaaag ctttaaaaga tgaagtcgaa
     1921 gccaatggcc ttaccttgaa agttattgag agtgttaatg ttcatgatga tatcaaaatt
     1981 ggtttgccaa cacgagatca atacatcgag aattataaac agacgattcg caatttggca
     2041 gaatttggga ttgaggttat ctgttataat tttatgccag tctttgattg ggtaaaatca
     2101 gatttggatt atcatttaga tgatgggtct tctacgttag cctatatttc aaaaaatatt
     2161 cctactgatc cagaggaaat tattagaaaa gtagaaaatt ctactaataa ttttgaattg
     2221 ccgggttggg aacctcatcg gttatcacaa atcaagcaat tattagaagc ctataaagaa
     2281 gttgatgaag cgaaattgcg agagaacttt gcatattttc tcaaagcaat tatcccgaca
     2341 tgtgaagaag ttgggattaa aatggcagtt catccagatg atccaccata ttctattttc
     2401 ggattacctc gtattgttaa aaatcgtgac gatttagaat ggttgtgtaa tgtagtggat
     2461 tctccttcta atggtattac gttatgtact ggaagtattg cagaacgacc agataatgat
     2521 gtctatgaaa ttctatctga attttgtcaa cgggatcgga ttccttttgc acatgttcgt
     2581 aatatcaaat atttaagtga taatggactc gatttttatg aagcgccgca taaatcagaa
     2641 tatggttctc tagatatgta taagatatta aaaacaatgt acgataatgg atttaatggc
     2701 tatattcgtc cagatcatgg tcggatgatt tggggagaaa ccggtagacc tggttatggg
     2761 ttatatgacc gagctctagg tgcagtttat ttaaatggta tttgggaagc gcttgaaaag
     2821 ggggcgagta aataattata taatggagtc tatttagaag aaaaattttc tcttctaaaa
     2881 actaagggaa ggaaaggagt gcttaaatat gtttttagat aagaactttt tattagagaa
     2941 tgaaatagct aaaagacttt ttcataaatt tgcagaacac caaccaatca tcgattatca
     3001 ttgtcattta gacccagcat gtttgctaga aaataagcaa tacaagaatt tgactcaggt
     3061 atggttaaat gataaaggag ctggagatca ttataaatgg cgattgatgc gagctaatgg
     3121 gatagaagaa gagttgatca gtggtgcagg taatgattat gaaaaatttt tagcttttgt
     3181 aaaaacagtg gagaaggcgc caggtaatcc tatttttgaa tggtctcatt tggaattaca
     3241 acgttacttt gatattgatt tgacaatttc tgagaagaat gcaccagaaa tttggcgacg
     3301 tgccaacgaa aaaatccaaa cggattccta tcgcccaaaa gaattgctga agatgatgga
     3361 tgtgaaagcc atttgtacaa cggatgatcc tacagatgac ttgcgatatc atactgagct
     3421 ttctaaacag gataattctt tcaaggtatt acctactttc cgtccagata aggtctggag
     3481 tatcaaatca tcaggatttt cgaactatat tttaaaactt agtcaagcag cgggtatacc
     3541 aatcaaaaac tttaatgggt tgaagcaggc attgagccaa cgagtggatt attttcatca
     3601 aattggctgt cgattagcag atcacggttt gaatacttat ttttatgaaa tagcagatga
     3661 aaaaatactc gacaatctgt taataaaagc tttagcagga aaaacggatt tccatttatc
     3721 agaaattgca caatttgtta caggtattca attacatttg atgtctgagt atacaagata
     3781 tggttggacg atgcaaatgc atttaaatgc tttaagaaat aattcaagta tattaaagga
     3841 acaaattgga gtagatgtcg gtggtgattc gatgggggat caagctagtt tgacacaaaa
     3901 tgttgtagca ttgttcgaca ctgctgaaaa gagtgggtgc ttaccaaaaa caattttata
     3961 ttcattaaat ccaaccgatt ggcttccact agcaacagca atgcaaagct tccaaggggg
     4021 tatgattcaa aaaatccaat tgggatgtgc ttggtggttc aatgatacag ctgaaggaat
     4081 gcgtcaacag ttgactacga tggcgcaaca aagtttgttg tcgaatttta caggtatgtt
     4141 gactgattct cgaagttttc taagctatcc tcgccatgag tatttccgtc gagtattatg
     4201 tcaattacta agcgagtggg ttaaagcagg tcgaattcca gatgatgaaa tgtatcttag
     4261 tcagatagtg gagaatattt gttatcgtaa tgcgtataat tattttaatt tttttgaaat
     4321 cgaaagtaga aaataataca aaaaataaaa gcttaaattt atttattgta agttagtaca
     4381 actagctaga gtatgtatcc ataaaagaag gagatagata tgaaaaaagt agaaatttta
     4441 agtaagttag aagcggctgg agtcattgcg gttgtacgtg gaaaaaataa agatgaggcc
     4501 attaaagcaa taaatgcaat tactgcgggt ggaattaaag ggattgaact aacttttaca
     4561 gttccgaatg ctgaagaaat aatcaaagaa attgctaaaa aatatgctga tcaaaaagat
     4621 gtaataattg gagccggtac agttttagat gcgtttactg ctcgtattgc aattatggct
     4681 ggagcagaat atatcgtaag tccatctttt gatcgacaaa cagcagagat ttgtaattta
     4741 tatcaaattc cttatttacc aggttgtatg acaattactg agataaagga ggcacttacc
     4801 gctggagtag aaatcattaa actatttcca ggttctgctt ttggtccatc tgttgtaaaa
     4861 acgtatcaag ctccattacc atatgtaaat ataatgccta ctggcggagt aagtctagat
     4921 aacttagaag agtggtttga agcaggcgtt gttgcagttg gtgtaggtgg gaatttgttg
     4981 gctcctgttg aaaaaggtga ttttgaagaa gtaactcgaa tagctagtaa atatatagat
     5041 aagttttatg agataaagaa ttattaggag aaaaaataaa taatactata aaatgtaaaa
     5101 aataaatact aactctttat acttcagagt atagtacgta agttggcagt tcaacagaat
     5161 gaaaacgctt aacaatagga aggagcgcaa attatatgga aagaaaattt ggtctaagag
     5221 acaaaatagg atatatgttt ggagattttg ggaatgattt cactttcatt tttgctagtt
     5281 cttttttaat gattttttat acaaaagtat tggggataag cggagctacc gtaggaacat
     5341 tatttttagt agcaagattt attgatgcag ttacagacat aacaatgggg cgtattgtgg
     5401 atggatcaaa accatctaaa actggcaaat ttaaaccgtg gattttacgg atgagtggtc
     5461 cagtcgcttt agcaagtttt ttaatgtatc aaacatcaat ggcatcagct tctatgagta
     5521 ttaaagttat atatatgtat gtaacatata tactatgggg aagtattttt tatacatcta
     5581 ttaatattcc ttacggttca atggcatcag ctatcacttc tgttgcagaa gaaaggacag
     5641 cgttatcaac atttcgtagt ataggagcta ccttagcagg tatgttcatt ggcgtgttaa
     5701 caccaatttt tatttataca agagatagtt ctggagctca aattgtacgt ggtggatcaa
     5761 cttttacaat tgtggcagga gtattttcag tgtgtgcagt tatttgttac gtaatatgtt
     5821 ataaattaac tgtagaacgg gttaaaagtg atgaatcgca gttaaaagtt tcattgttga
     5881 aatctttaaa aggattagct aaaaatagag cgttgctttc tataatagct gcagctattt
     5941 ttttgttgct tgctcagctt ttaacaatga gtatgaacaa ttatgtattt ccagattatt
     6001 ataataatgc acagggaata gttttaatga actttatcaa tcctattttg atgttggtac
     6061 ttgtttcacc tgtaagttta attttcagta aaaaatacgg taaaaaagaa ttggcgtcag
     6121 tagggatgtt cttctctgct attgtatatt tacttttatt tttgataaag cctagtaata
     6181 tgtatgtatt cttagttctt acttctgttg gatatatggg gttaggtatt tttaatacag
     6241 tcatttgggc gaatattact gatgtaatcg atgatcaaga agtgaaatct ggccaaagag
     6301 aagatggaac tatttatgca gtatattctt ttgcccgaaa aattggacaa gctttagctg
     6361 gcggtgtcgg aggttggact ctttctataa tagggtatga ttcgttagca aaagtacaaa
     6421 gtggagaagt tttacaaaag ctgtataact cttcaacgtt gattccagca gtttgtttct
     6481 ttgttgtagg attgatttta tttttcgtat atccattatc taaagaaaaa gtattagaaa
     6541 attataattt gctaaatcaa aaacataact aggagaaaaa acatgaacag ttggatattt
     6601 tgtattgatt cagatggttg tgtcatggat acaatgactt ataaacacaa tctctttttc
     6661 gggccaattg ctgcagagat ttttgatgtt catcataggg aaaaattcct acaggattgg
     6721 aataatatta atttatattc agagactcgt ggaataaaca gatttttagg tttagttaaa
     6781 acattagaaa aaaatgatgt accatcgatt aatcgattaa agcactgggt atctgtaaca
     6841 aaagaattgt ctaatccatc tttgaagagg gaaatagaat actctcagtc agaggattta
     6901 ttaaaagctt taaaatggtc ggaagaagtc aatcgacaga ttagtcaagc taaaggatta
     6961 gataaaccct ttttaagtgc tagagatact ataaaagctc tgaaaaaata tgggaagata
     7021 attattgtaa gctcagctaa taaggaagct attcaagaag aatgggaaag acatgaatta
     7081 ttatccttag ttgatgaact gtgttgtcaa gataaaggaa aaaaagaaga cattattcga
     7141 agtgtaattg aaaatggttg tgatttggat aagatattaa tgattggtga ttctcctggt
     7201 gatctagaag ctgcaaataa gaataaagtt ttcttttatc ctattttggt taataaagaa
     7261 aaagaatcat gggaaaactt aagaactgat attttattta aatgtttatc gggagagtat
     7321 atagatattg aacaaaaata tatagatact ttttggaaaa atcttactaa taagtagagg
     7381 agaaagaaaa tgactataaa agtagactta cgaaaaaaac catatcattt agatgaagat
     7441 gctatttctt ggattgaaga gacgattgat gctatgactt tagaagaaaa aataggtcaa
     7501 ttatttgtta atatgggatc tagtcgtgat aaaaattact taagtagtat ggtcaatcag
     7561 taccatattg gagctgtacg atataatcca ggaactgctg atgaggtata tgatcaaaac
     7621 tatatcctac aaacagaaag caaaattcct ttattaattg ctgctaatac agaagcaggt
     7681 ggtaacggag cttgtacaga tggtaccgaa gttggtgtag aagtaaaaat tggtgcaaca
     7741 aatgatgcga aatatgctta tgaattaggg agagtttctg gaattgaagc atcggctatt
     7801 ggatgtaact ggagttttgc tcctattgta gatattaata gaaattggag aaatcctatc
     7861 atttctagtc gatcttttgg acaaagccct gataaagttt tagaacttag tttagcttat
     7921 atgaaaggta ttcaagagag tggaattttg cctgcagcta aacatttccc tggtgacggt
     7981 attgacgaac gagatcaaca tctatctttt agtattaata gtttaagtac agaagaatgg
     8041 gataattctt ttggaaaagt atactcaggc ttaatagaag ctggattacc ttcaattatg
     8101 gcaggtcata tccatatgcc agcttattca aaacatttta atcttgatct gaaagatgaa
     8161 gatttacttc ctgcaacttt atcaaaagaa ttaatcactg acttacttag aggaaaactc
     8221 gggtttaatg gattagtagt tactgatgct agtcatatgt taggaatgac tggggcaatg
     8281 aaacggtcag aattattgcc cacttctatt gcggctggat gtgatctatt cctatttttt
     8341 aatgatccag atgaagactt taattatatg atggaaggtt attacaaagg aattatttca
     8401 gaagagcgtt tacgtgatgc attaagaaga attttaggct taaaggctaa actaggtttg
     8461 cataaaaaag aaaagaataa aattttgtta ccgaaacaag aagctatgga aaaaattggt
     8521 ttaattgaaa ataaatcaat ttttaaagag gtagctgatc aagcaattac actagttaaa
     8581 gataaacaag atatttggcc tattgtacca gaaaaatata aaaggatttt actggtagat
     8641 gtacatgggg taaaaggtgg gtttggtgaa gtagttgctg gaaatagacc tcaggccatt
     8701 gatttattga aacagatttt gtcagcaaaa ggatttgaag ttgaaaaata catttcacct
     8761 gaagaagaat tgaaaggatt atcgcaagaa gaaaaaatta aaaatatagc tggaatatat
     8821 gctgctaaga gaccaatcaa agatttaact gaaaaatatg acttgattat taatatagct
     8881 aatgttaacc caggtacagt tcaaagaatt gtttggccag caagtaaagg tactccggat
     8941 attccatttt atattcatga aattccaaca atttttgtat ctgtacagtg tccgtttcat
     9001 ttagctgatg tgcctcaagt aaaaacttat atcaatactt atgatggaaa acaagaaact
     9061 ttagaaatat tagtggacaa actaatgggc ctttctgaat ttaagggaat aagtccaata
     9121 gatgcttatt gtgggttaaa ggatacattt atttaatatt taaattatat aggagtgatt
     9181 tacatgttat atcccattat gacagagacg aggcaagtaa ttgatttaag cggtctttgg
     9241 agatttaagt tagatacaaa tgatgactta caagaagaac ttgctttaga accaatggtt
     9301 gaaaaagaga gttatccaat ttcagttcct tcttcttata atgatttatt tgaaacagag
     9361 aacattagaa atcatgttgg atgggtgtgg tatgaaaaag aagtgattct tcctaaaaaa
     9421 ttattagaag aaagattagt tcttcgtttt ggatctgcaa cacatgaagc aaaagtattt
     9481 ttgaatggaa aattgattaa tcaacacaaa ggagggttta cgccatttga atcagaaatt
     9541 aatcagttta taaaagatgg aagtaacaga attacagtgg ctgtaaacaa tgttattgac
     9601 gatagcactt tacctgtagg gattttgaaa gaaacgatac aggaagatgg agacataaaa
     9661 aaagaaaatt tggttaattt tgactttttc aattatgcag gcattaatag acccgttaaa
     9721 ttgtatacaa ctccgaaaga ctatataaag gatgtcacta ttaatacaaa aataattgat
     9781 tcagatgcaa atgtagaatt taatatcgat acagaatgga ccggagaaat tctaatagca
     9841 atctatgacg aagaagctca acttatagca gaagctaagg ggaaagaagg agtgattcaa
     9901 cttaagaacc caacactttg ggagcctcta aatgcttact tatatagtat gaaagttagt
     9961 ttaattaacg aaaataaagt gattgatacg tacacagaag aatttggcat tcgtagtatt
    10021 caggtttcga atgggaagtt tttgattaat aataaacctt tttactttaa gggatttggg
    10081 aagcatgagg acacgtatat taatggtcgt ggtttgaatg aagcagtaaa tatcaaagat
    10141 tttaatttaa tgaaatggat cggtgctaat tcatttagga cttcacatta tccttattca
    10201 gaagaaatta tgcgcttagc agatcgagaa ggtattgtag ttattgatga agtacctgca
    10261 gtaggattac atttgaattt catggctact ttattagatg acaacgtaga aaaacatgat
    10321 acttggaaag aaatacggac agaaaatgct cacaaaatgg cgattaagga attgatagaa
    10381 cgcgacaaga accatccgag tgtcgtaatg tggtctattg caaatgaacc agattcggat
    10441 tctgaaggcg ctaaagaata ttttgaacct ttagttgaat tgactaaaga acttgaccct
    10501 cagaagagac ctgtaacaat cgttacttac ttaaagtcaa caccagatat ttgtaaagta
    10561 ggagatattg tcgatgtttt atgtttaaat cgctattatg gttggtatgt ttcaggtgga
    10621 gacctaaaac atgcaaaata tctgttgaaa aaagagctag atggatggat ggaaagatgt
    10681 cccgatacgc caataatgtt tacagaatat ggagcagata cagtagctgg acttcatgat
    10741 acagtaccaa caatgtttac agaagagtat caagtgggtt attacgaagc aaatcacgaa
    10801 gtaatagata aatgtgacaa ttttgttggt gaacaagtgt ggaattttgc agattttgaa
    10861 acaagtcaag ggattttaag agtgcaagga aataagaaag gaatctttac tagagataga
    10921 aagcctaaaa tgattgctca ctatctaaag aaacgatggt catcaatacc aaatttcagt
    10981 tataaaaaat aatttacctt ttataaggaa tataaaaaag gagtggctgc ttaattgcca
    11041 actccttttt tttataaaaa gattttctgt ttaaagaagg atattttttt ctagtttttt
    11101 cgaaaaaaag gcgctttctg attgttgtat tagtggaaag gggaaaaaag gagcatattt
    11161 atcatgcaac aaaattacaa tgagaaatct atacaacatc aatttgatgc ctactgtaaa
    11221 aaagtattac gaaacgaaac taagtctatt cgaaaacgaa acattaacat aacagataat
    11281 gaggaaccat tgacgaattc aaatgaaggt aagtactccc aaactcattt tgatgaacaa
    11341 gatgtctatt tcttatttgg aatggaaata ttaatttcag atcaaaagtt atcggcagcc
    11401 attgatcatt tatcggaaat aagaagaaag gttatcctac tttattattt tgcaggtttt
    11461 aatgatactg aaatcggtaa aatcctcaat atgagtacaa gtggtatctg gtatcaacga
    11521 aagaaggcag ttgaacaatt aaaaatgggg tatgacttat ggtagacaac cccaaaaaac
    11581 ggcagtatcc attagttcct ctatccatta tccaatctgc tctagaagaa gatccaattt
    11641 caatcaagat gatactcaaa cattatcata gttatatagc taagttatgt ttgacaaatg
    11701 gatttaatga ggcggggcaa tttattacct atgtagatga gtatatgcta agacagctag
    11761 aaattaaatt gatagaagcg atcttaaaat ttaaaattaa ctaactaaaa agtccccttt
    11821 ccacttattc tgtttaaaag aatcttctaa tagttaaagg attagtagat tcttttaaac
    11881 agaaaaccta ttgatcattg aaatttaaat acattctatc agtacgttaa cacttcaagg
    11941 agcatatttt agttgcgcca tgatttctta ctgtaaagaa ggagcgataa acattctaaa
    12001 ataattttta tagtcaataa aaagcgatga tttgaagttg ggataatgat acttccgtaa
    12061 ggctcgccgt aaacttggag agaggtgaaa ttcctatgtg cttttgacaa aagcagctga
    12121 ttaagaatta tttccatatg ttgatttcaa cattttttct attattttta agaccaaatt
    12181 cagtgaggca aatagcatca tgtaaattta gtttttctat tgaacgttct ccacttttta
    12241 gtttttttat agtactcaaa tttaatcctg tatgtttaga gatctcattt gcagtatggt
    12301 tttgtaaagt ttctcggatt attgttatat tagcaatttc attacgcata attatgttcc
    12361 tttcttatca aaaatatcaa tgactacagt gggcttttag tgaaaaaaag gggtactaca
    12421 gtctcttttg aaaacaaaaa aatacagtat atacgttttc taaatgctaa gcaccaacgt
    12481 ttacactgta tttaagggtt tggcacccaa tgggctgtga ggggctcgaa cccgcgaccc
    12541 gatgattaag agtcatctgc tctaccaact gagctaacag cccaaattaa tactttgtta
    12601 ttctaacacc taaaataaaa aagtgcaata cttattatca gaattgagag tgaattttat
    12661 ggattttcaa gattatgaaa aaatatttga aagttatcca tcgatattga aaattactga
    12721 tttaactaaa atgttaaata ttggaaaaaa taaagcttat aatttgctga aagatagatc
    12781 aatagaaaat ataaaaattg gagcaaggta caaataccta aaaaagctgt cattgagtat
    12841 atattgaaaa tacaagatga aggtaaaaat catgctataa taaaatagtc aaaatttttg
    12901 ccgatatttc agtttaagag gagagattga attgaaatta agagcaaaca taattgaaaa
    12961 aagaggaaca ttacaagtag ttgtatctta taaagactct aacggaaaaa ttagacaaaa
    13021 gtggaaagat acaaaattaa aaaagaaagg aaataaaaaa cgagcagaaa aaattagaga
    13081 tgaattttta ctagaattgg aaagtcaatt agctatggaa tgtgaatgtg ctaatccaaa
    13141 tttattgttt tatgactatt tgcttaatta tttggaagta tcaaaaaaac aagtagcatt
    13201 taacacatat gttgggtata agcattacgt ccataatcga atttacaagt ttttttat
//
LOCUS       DACDTO010000083        13185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDTO010000083 DACDTO010000000
VERSION     DACDTO010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.083"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1537
                     /locus_tag="IWK70_002292"
     CDS             <1..1537
                     /locus_tag="IWK70_002292"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9614797.1"
                     /translation="DASEYKNYLLGLIFYKYLSDRLLEQVVLLADESLEEYDTVSKQT
                     MLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLADQAKQATFQLNDLNKAFVQL
                     ASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKKLNDVDVLEHDGDVIGDAYEF
                     LISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRN
                     YLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFD
                     AVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVL
                     PHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLF
                     IDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRY
                     VDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFK
                     HEQ"
     gene            1527..2744
                     /locus_tag="IWK70_002293"
     CDS             1527..2744
                     /locus_tag="IWK70_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9614798.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2757..5906
                     /locus_tag="IWK70_002294"
     CDS             2757..5906
                     /locus_tag="IWK70_002294"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAP9614799.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(7841..8506)
                     /locus_tag="IWK70_002295"
     CDS             complement(7841..8506)
                     /locus_tag="IWK70_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9614800.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            8632..10668
                     /locus_tag="IWK70_002296"
     CDS             8632..10668
                     /locus_tag="IWK70_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9614801.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            10672..11310
                     /locus_tag="IWK70_002297"
     CDS             10672..11310
                     /locus_tag="IWK70_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9614802.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            11338..11766
                     /locus_tag="IWK70_002298"
     CDS             11338..11766
                     /locus_tag="IWK70_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614803.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            12223..13134
                     /locus_tag="IWK70_002299"
     CDS             12223..13134
                     /locus_tag="IWK70_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9614804.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         4578 a         2083 c         2565 g         3959 t
ORIGIN      
        1 ggatgcgtca gaatataaaa attatttatt gggactgatt ttctataaat atttatccga
       61 tcgattgttg gaacaagtgg ttcttttagc agatgaatcc ttagaggaat atgacacagt
      121 aagcaaacaa acaatgttgt atcgtgaatt actttcagat gaagaatcaa aagaagatct
      181 gattgccaca atcgttgata ttttgggata tgcaattgca ccagagtatc ttttcaacgt
      241 cttagcggat caagcaaaac aagcaacgtt ccaattgaac gatctaaaca aagcctttgt
      301 gcaattggca tctacctaca atcaatttaa tgggttattt gatgatgtgg atctccaatc
      361 aaaaaaacta ggaacagatg agcaacaacg aaatgtgacg attaccgaag tcatcaagaa
      421 attgaatgat gtagatgttt tagaacacga tggggacgtg atcggggatg cgtatgagtt
      481 tctgatcagt caatttgctt cagaagcagg gaagaaagcc ggagaatttt acacacctca
      541 tatggtttct gatatgatgg cgcaaatcgt aacgctcgat caaaaggaac gacgattctt
      601 tagtgttttt gatccaacga tgggttcagg ttcattgatg ttaaacgtac gtaactactt
      661 aacacaccca gataacgtta aatatcatgg gcaagaacta aatacaacga catacaatct
      721 agcaaaaatg aacttgattt tacatggtgt cgacgccgaa gaaatgaatc tccgcaatgg
      781 ggatacgttg aataaggact ggccaacaga tgaaccttac acatttgatg cagtagtaat
      841 gaacccacct tattcggcta attggtcagc agatacgact ttcttagatg attctcggtt
      901 taatcgttat gggaaattag cgccaaaatc caaagcagat tttgcgtttc ttcttcatgg
      961 gttctatcat ttgaaggaaa caggtaccat ggccatcgtc ttaccacacg gagttttatt
     1021 ccgtggcgct gcagaagggg tcatccgtca aaaattacta gaagatggca gtatttatgc
     1081 tgtgatcggt atgcctgcga acttgttctt tgggacatcg attccaacga cggtgattgt
     1141 gttgaagaag aatcgtcaaa ctcgagatgt attattcatt gatgctagtc gtgaatttgt
     1201 taaagggaaa aatcaaaata aactatcgga agaaaacatt caaaaaatcc tcgaaaccta
     1261 cgctgaaaga aaagatgtcg agaaatacgc tcatttggca acctttgatg aaatcaaaga
     1321 aaatgactat aacttaaata tcccacgcta tgtagatact ttcgaagaag aagaaccaat
     1381 tgatatggtt catgtcggaa atgacatcaa gaagatacga caagaacagc aagtgctaga
     1441 aaaaaaactt ttagaagcta tctcttccct gcaaacaaca cctgaaaatg aagcgtggtt
     1501 acaaggtgcg ctggaggtgt tcaaacatga gcaatgatac acaaccagaa atccgtttcc
     1561 caggatttac agatgattgg gaacagagga agttggggga cttcgttgta gattatgttg
     1621 aaaagacaag tgtacaaaat caatttccta tgctaacttc atctcaacaa aaagggatag
     1681 ttttgcaaga agactatttt gcaaatcggc aagtaactac agaaaataat attggttact
     1741 ttgtactacc tagaggatat ttcacgttcc gtagtcgaag tgataatgat gtgtttgtgt
     1801 ttaaccgtaa tgacattatt gatagaggga ttataagcta tttttatcct gtatttactt
     1861 taaaatctgc tgattccgac ttcttcttaa gacgtattaa caatggaatc caacgacaat
     1921 tatctattca agcagaggga acaggtcaac atgttctgtc tctaaaaaag tttaaaaata
     1981 tagtagcaat gtttcctagt gaggaagaac aacaaaaaat cggcaccttc ttcaaacaac
     2041 ttgacgacac gatcgctctt catcagcgta agttagattt gctgaaggaa acgaaaaaag
     2101 gattcttaca aaaaatgttc cctgaaaacg gagcaaaagt gccagaaatc cggtttcctg
     2161 gttttacgga agattgggaa gagcgtaagt tgggagaatt aggtaaaact caatcgggaa
     2221 ttggatttcc tgatgccgag caaggtggtt ctgaaggtac tccatttttt aaagtttccg
     2281 acatgaataa cattggtaat gagtatgaaa tgagaaatgc aaatcattat gtatctaatg
     2341 agcaaattga gcgtaaaaaa tggaagccaa taaaagatgt gccagcgatt atatttgcta
     2401 aagttggcgc agcaataatg cttaatagaa aacgtttggt cacaagccct ttcttaatag
     2461 ataacaatac catggcttat ttatttaata atacttggga tatctatttt ggtaaaatat
     2521 tgtttgaaac tataaatttg ccaagatact cccaagtagg ggcattgcct tcttataaca
     2581 gttctgatat tgagaatatc agtgttaagg ttcctgtaaa ggatgaacaa caaaaaatcg
     2641 gtactttctt caaacagcta gatgatacga tcgctcttca tcaacgtaag ttagatttac
     2701 taaaagaaac gaaaaaaagc ttcttacaaa agatgtttgt ctaggaggga agaagaatga
     2761 aaattagtca ccgtgaggaa gcagaagtcg aagaacagtt gattcgtgta cttggagaag
     2821 ggcataatca gtggacgtat cgtccggatc taaaatcaga agaagacctt tgggtcaatt
     2881 tacgccaaaa aattatctca aataatcaag cagaactgaa tgattttcct ttgaccgata
     2941 aagaatttga aacgatcaaa acggaattac tgctgcgaac gaaaacccca tttgatgctg
     3001 cgaaatggtt aaaaggtgaa aatgggatgg ctcggatcac gattgaacgg gaagattcgc
     3061 aattgggttc tgtctcactt atcctttatt ccaatcaaga cattggtgga ggaatctcta
     3121 cttatgaagt ggtccatcaa attgcaaaac gtggaagtaa catagaagca cgggatcgac
     3181 gttttgacgt caccttatta atcaatggat taccgattgt ccaaatcgag ctgaaacaag
     3241 taactgcaaa agatggtgtt tatcaggcat ttaaccaaat caaaaaatat gcagaagaag
     3301 gtatgtttcg gaacaatatc ttttcgacct tacagttgtt tgtggtttcc aatgaacaaa
     3361 ccactcggta ttttgcaaat gccttaccga aagacttgca ccctaagttt ttatttagtt
     3421 ggcgaacgaa agacaatgaa aaagtagaaa acttatatga gttttgtaaa caagtgttaa
     3481 atatcccgga tgctcatcgt ttgattgcgg attatacaat cgtcagtgaa gatcaagaca
     3541 ataaaaccct catggtgtta catccttatc aagtacatgc gattcaagcc ttgtttattg
     3601 ccgcaaataa acatcaatca gggtatgtgt ggcatgcgac tggttcagga aagacgttga
     3661 ctagttttgt atctactaag cttttagcac gaaaatcggg gatcgatcga acgatcatgt
     3721 tagtggaccg caaagattta gataatcaaa ccaccacaga gtttacaaaa tttgcttcgg
     3781 aatttaatac tgggatttct tccgggaacg ccaaagcgaa tagcttgatc gttggaacgg
     3841 gaagtgccaa agaattaagt gaaaccctgt tggcagatgc caatgcaaat gttgtaatca
     3901 ttacaacccg tcaaaaacta gatgcggcgt tgaaatatgc aaagaaacaa gaagagaaaa
     3961 aaggaaccaa tcgcttccaa aaattaatgg gccaacatat cgtgtttgta gtagatgagt
     4021 gtcatcgagc gttaagtgct gaaaatatgg aagaaatcaa aaaaatgttt ccgaaatcca
     4081 cttggtttgg ctttacaggt acaccgattt ttcctgagaa tcggaaacaa gccaaagggc
     4141 aattggcacg tacaacccat gatcagtatg gagaggtctt gcatacctat accattaaga
     4201 atgcgctaga agatggctct gtcttaggat ttcaagtaga gcatgaaaac accgtggagc
     4261 cgacttcact agagaataaa atctatcgga aattaaaaga agtagaaaca tatgcagagt
     4321 attcatcaga acagatcaat cgtatgattg atcaaatgga acctgtcaaa aaagaaagtt
     4381 atttggaccc agctgttttt gaagcagatg aacatatcca aaaagtcatt cataaaatgt
     4441 tccgtccaga taatgcctat acaaaatttg attttcgaaa tggcaggcca acgaaatctg
     4501 caatcttaac cacgagttcc attgacatgg ccaaaaagta ttatcgggcc atcaaagaga
     4561 tgacgaaaga gcctgattgg ttaacaaaag agtttcccaa tcagctcatt cgtgaaggac
     4621 gaacgatgga ggatcctgat tttccacgaa tcgctattac ctattcatta gaagaaaata
     4681 gccgagattc aagtgcccaa caagaagaaa tgcaaaaaat tattgaggaa tataatcagt
     4741 attatgatac ttcctggtca ttagcagaca ttgagcgtta taacggagac atcaataatc
     4801 gtttggcacg aaaaagagcg gaattcaaac aatttgggaa acaaatcgat ttagcgattg
     4861 tagtggatcg tctattaaca ggttttgacg cccctacgat tcaaacccta tttgtggatc
     4921 gtaatctaga gtatgcagga ttgatccaag ctttttcacg tacgaatcgt acgtatcctg
     4981 ataagacaaa aggattgatt gtgacgtttc gtaagccagc gacaatggag aagaatgtcg
     5041 aggatgcgac aaaactgtat tcaaaagcaa aagaagaatc aggtttgatt tatccaagtt
     5101 atcaggaatc caaaaaaaga ttcaaacaag catatggtaa gttaaaagag gtttcatcca
     5161 tggaatccat tgatgaacat acgccacttg aaatccgtat cgagtacgtc aaagcatttc
     5221 aagaattgaa tcgtgcttat gaatcgttag tgacttatga cgattacaat gatgacatgg
     5281 tgaaatctcc aactttgaac aatcaaattc aacttttgga agaacaaatt ggcgtgtacg
     5341 agaccgtgaa gggttcatta atagaggaag aaccagaaaa agaaggagaa gatttttcaa
     5401 cgatcgaatt ttatagtgaa aataccacta agttgtatga tattgattca acttatattg
     5461 atcaattact aggaacttat gctgcgaata gttcagatgc acgagaagaa attgaaaaag
     5521 ctttggctaa attgaataaa acagaaggag tcaaagaagt ttaccgacaa attttaaatg
     5581 catttgatgt ggggacttta gatagtaatg aagatatctt tgtcattaaa cgtcgctatt
     5641 ttacacaagc gagtgatgat ttgattcatg tcttttccaa tgagtggttt gtttctgaga
     5701 aagaattaca tgcttctaat ctgcaatatc taccaggtga agatccaatt cctaatatga
     5761 aagcaattat caacagtaag gattacgaag gatataaagc caaacatcca gaagcgaagc
     5821 catttaaata cccacaagaa atgaagcgag catggcgtaa aatgttagat gatgagttga
     5881 ttccacttga gaatgaattg aggtaaacga gaagagttaa gaaaattgta cgattcaaag
     5941 atggtagtca agtggaagtg tagaaaatag ggatagatgt cttttatttg ttcacatttt
     6001 ataacgaagt atttaagaca gctattgaat aatgtttcct ccaccttttt cattcattta
     6061 ccccaccccg ttgttatatg accgtgtttt gagaggaacc aataaaaagc ataggctaac
     6121 taggggttcc tttttctttt gtttttttgg aatccgctta agtgaaatgt atctgaaatg
     6181 aaacggagat aaagtgattt tttgctataa tgacaaggtt ttagctgttt cagataccat
     6241 gtaacccaaa atagtttatt gagaaaatag gagagtattt gatactaact agtaggaaaa
     6301 aatgagataa aaattaaatg gaatcatttc aggtaatctc aaataaaaaa atatacagtc
     6361 ctgtggcact ttacctatgg gactgtatat tttttactct cagcgggctt gttttgactc
     6421 cgtgtctgac tccgtcgtat tgattttaaa tgatattcga tgaccattta acaggtcatt
     6481 tttgaaattt ttgtctctat atgactcagt ttgataatta ttgatagatc aatttgggtc
     6541 tatatcgaac aagggaataa tccaataaaa aatgatttag aatatcaaaa taacaaaaaa
     6601 gaggactacc tgtattcgtc ctcttttttg accagatttt gaccagtcta atttacattc
     6661 taatatattc atatgagcta attttttgat taaatggtaa gtttttcctt gtttgtttta
     6721 gtttaaacta gtgtgtaatt taaaaatcta tatggaaggg tctgtggggt aatacttaaa
     6781 taaaggctag gaacctgata acatcaaggt tcctagcctt tttataataa aatgaagcga
     6841 aaataatttc ttttaaagag ccgagattat cagctatata agagtgaatt tgatgcaaaa
     6901 aacagagccg aaacagtctt ggtgttcgta gctctgctta tatgtttcga atttaagaca
     6961 aagagttggc aaaagatgtt gataaggaag ataagataac tgtgttatat aaactaggta
     7021 actaactgta acaagctaat tttaagagat tcttattaga agacgtttag gaatataact
     7081 aagaaaatga ttatacaggt aattgtcatt tcaataatgt tttctaattt tcgcgtcttt
     7141 gttttaagaa aaaggccaga atcagttgga aaaaagttcg tacgatagtg aagaggggca
     7201 aaatttccta ctaatattcc cactaacaag agataaaacg gccaggttgg tgtgattcct
     7261 ctataaaaaa atgcccatgc taccgaaatc ccatagatta acaactgaac aatttttaca
     7321 atgaaaaaat tgttctgcca ttactcaaag tctcttcctc cttgtatcgt ttatttgttt
     7381 cattttatca ttatgatagg cgcttgaaca atccattttg tattttattg agtactcttg
     7441 gataaactac cagcgattat catatttatt tatgttactg taagccatca tataaaagta
     7501 aatgagcaga tcatagaaat ataaaaaaga actaaaattg atctaaatta tgtgcagctt
     7561 gactacgtac caatctatat tacttactag attggtcgtt aatctattcg gatcgaatga
     7621 gcaaaatatt tttgtgaatg tccatccttg attatgattc caaaaagaat cattgggagg
     7681 ctgtggcgta aatattatac tataagccag aaatacctat cattctgaac ttttttattt
     7741 aggaactttc atctcaaggc tttttcaatt aatttgtacc cctgtttttt ggataagatc
     7801 atgtgcattg attttttcta tctctacgtt catctcctac ttaatgccta tctggctcct
     7861 gagggcttct ttttaaggcg tcgtcatagt cttggctgag gatttcttgg taatctgagg
     7921 gaatggcttc ccaagtgagc aggcgaacgt tcatgacgtg ttttcctttg tgtttgatta
     7981 cagcatggtt tttctctttt tctacctctc cagcgtgaaa agacagccat gtaccatggc
     8041 caaagatatc attacatacc caacgttcga tggaattgtc cacgtatcct acagggagtg
     8101 tggtatagcc gatttgtggt ttcaaaaaag gattgaatcg atcgatcgtt tcatttttgt
     8161 attgataatt gataagaaaa agatagacca aacaaaagac gagcagaaag aaagatagtg
     8221 tctttttgag tgagaagggt tgttttgatg aataagattt cttgttaaca agaggttcat
     8281 ctggcaatgt tggttctatt gattcggtag ctgattcaat agaaggaaaa tcaatcggca
     8341 gacctaaagt atgatgtaaa agtgacagca tttcaaggtc tggcattgta cgagaatttt
     8401 cccaactaga aatcgtttta tcggaaacac caattttttc gcctaattct ttttgcgtaa
     8461 gcttacaatt tctgcggtaa gttcgtattt tttctccggt gttcatcttc ttctcctttc
     8521 gattctacag tatatagatt tattctacat gacctataca cttgatagat acgttttttt
     8581 gatgaaaaac ggtatgattt tatcaaagaa tcatttaagt aagggagagt ggtgataatc
     8641 aaaaaaaagt tcacagtttt actcacaatc atagtgaccg gtatcatgag tggttttgcc
     8701 ttggttagta taaataaaca ggtactctat cggacaaatg aatttttcca tcaaacagat
     8761 acttatactc cgattcgatt gccacaacat tttattgggc aagaagaata tgagcagcta
     8821 atcatggtat ttgaaaatgt ctcatcagaa actgagcttt cttatatgaa aaaaacagta
     8881 aatgaatcca atgaagtaga tacatcttgg aacttcaaga tacagccaaa gcttgaaatg
     8941 acattgtatt atcaagggga tgtcaaatct tctcacgaga aggtacccta ttctaatacc
     9001 tcctttcgcg taacgtccat tgaagaatcg atcaaaataa aagattttga aggtgaattt
     9061 ttcttgatga cgagagaaaa agaagcctat gaacggttca tttcactatt cactgatcga
     9121 ttcaatcaag catttcacac tgattatgcc actaatattt ttgaagactt tgaaaatagc
     9181 tattcgtatt cggttatttc aaatacagac gtatataatt atcgagtgta tttaactatt
     9241 ggattgattc ttttattcct tttgttatca tcctttttct tattgatgaa taaagaaatc
     9301 agtttattgt tgaataatga cttttcatta catcaagtat tgtggtgttt attggggaaa
     9361 aagaaaatca ttattacatt cataattgca ggaatcattg gtggttttat ttttatgtat
     9421 tataaggagc aatttcttta tttgttccag atgtacgttt tcttgttcag cggaatatat
     9481 ggtatctctt tgatttctct tttattagtt gaaaaaatcg cttcgttaag agaagcgttc
     9541 aactatatcc aatacattct ttttacattt ggattgttgt tttctcttct tttgaccgtt
     9601 gccaatgtcg aattagcaac tatcgttttt tcttcttcac agttagctgt ttcccatgaa
     9661 ccaccaaaag aaatcgctgg tgaggactat catgtatttt atccagtaac catcgggaaa
     9721 aaccacgtgg attttattta ttccaatcgt tttgcatcag cagcagatca ggaattatat
     9781 gaaactttga ataaaaacgg aagtatatta gtcaatgtat cagattactt gaatgaagaa
     9841 aatgaacagt ttggcagagg gataaaaata aatctgaatt atcttaagaa atttcctttg
     9901 attgatgtct ctggaaggtt gatccaaata accgaaaaag aaacgcaccc tgttatattg
     9961 atccctgaac gatatagaca tacagattta aaaaatgacc tggcgcaaat aacagaatat
    10021 tatcaagaaa tcagtgaaaa agaaccgaaa tttctattta taaaaaacaa tcaacccatt
    10081 tatacattta ttccttctat cccctggatc gaacattatc cggttgttga aatactgaca
    10141 ttgaaaaaca gtatgtattt ggaaagaaat attctcagcg gagaaatcta tccaccgtta
    10201 aagatcaaga taggctcttt gtcgaaagaa aggttatttg agctgattga agaaagtcag
    10261 ttacgtgata acttgcagtt atcatttccg tatactcaga ctgaagagat agctgtcaaa
    10321 gtgttaagca gatcatttca ttacttgata cagatatttt tactcacttt cttttctttc
    10381 ttcttgttga gctatgtcat gttatgcata tattttgtct acaattgtcg gaaaatcgct
    10441 atctttcgtt caagtggtta ttcgctcttt gaaacgtata aagatttctt catgatgaac
    10501 ctcatcaaat ggggaacgac aagtgtaatc ttcctttttc ttatagaaag agagcctaaa
    10561 tatcttttta atattttttt ctttagcttc attgatttta ttctttcagt tgtttttatt
    10621 ttgtctacag aaaagaaaag tcagttattg ctgatgaatg gaggttaaca attggagccg
    10681 ttgatagtga taaaaggtgc gaaaaaaatc attcgaaaca aaacgattct tcatttggat
    10741 cgtttcactg cggatgctgg ggatcttgtg actgttgttg gagaaagtgg agcaggtaaa
    10801 agtacgttat tgaatatatt aggattgaat gatcagttca gttcaggaca attctatttg
    10861 ttcgatcaag agacgaaaca attaactggg agagagaagc gcttgataaa aagaaatacc
    10921 atatcttttc tttttcaaga ttttgggttg attgaatatg aaacaatcga ttttaattta
    10981 gatattggat tgaagttttc gaatttaaat gtttcagata agcgaacagc aaagtatgag
    11041 gcattgaaaa aagtaaatat aaacaagaag ctaagtacgc ctatctacac attatctggt
    11101 ggtgagaagc aaagaatcgc ctgtgcgaga gtcttattga agaatagcaa aatcatttta
    11161 gccgatgaac cgactgggtc attggataaa agaaataagg ataaagtagt tgaaatctta
    11221 tcgcaacaag cttcccaagg tacgttagtt atcgtagtca cgcatgatcc atatctgacc
    11281 aaaataggaa acaagaagat catgctttaa aaatcaaaaa ggaaaggatg tcgttttatg
    11341 aaaaaagcag ttattatttg tgcactaggg ataggttttg ttggaggaaa cgctatgtca
    11401 tctgaagtgg aagcaaattc agttcacggt gaaatctatt acaaatcttt aaagccagga
    11461 gagattcatt ttgatgctaa aggtaatatt attaagtcaa atttaccagt agaccgggaa
    11521 atttctagaa cagcaattaa tcatagctct ggaagatggg tttattatag tgatacaact
    11581 tgtacgagaa aaacaggtca ttctaaccac tatagtcgtg tctataatcg tcacggtgca
    11641 aaagcgaaag ttggaaactc tattcgatca gcaaatgcaa caaaaggagt atgggcctat
    11701 gcaagtgcga caggaaatag aactgacaca ttctcatgtt ggtacaatcc aaccggttgg
    11761 tattaagtat ccaaaagttt atataaaata gaaaaaacat ataatgaaac agcgttaaaa
    11821 gttatttttg gtagttttaa tatattcatt tttagttgta taaaaatata tcataataaa
    11881 aacaacaaaa aaatgtctgg aaaatcgaac ataaaagtgt ctgatttgaa tatcccagca
    11941 gcggaagaag aaaaacaaaa tcgataacat ttgttctaag aattagttac gttaaatacg
    12001 gaacgcaaga tagacttgaa aaacggtaac aaaagaagta gtatattaag gtgtgatgtg
    12061 ctacttgaca tttattctgc tgagcgaact ttctggattg tgtagcctta tatctattca
    12121 ggacgtatcg agcagattag taaaggtgtt cttgatgatt ttcatcttga acacctttgt
    12181 ttttagcaat tcgcaaaaaa cgaaaagtag gagaattgat aaatgaaaaa aagattttta
    12241 gcacttgcca tcgtactagg cactggctta ttgtcaggct gtacgaatgc cggtgaaaaa
    12301 acagccgtta gttataaagg cggtacaatc agcgaacaag aagtaatgga tagcttgaaa
    12361 aaaatgcaag gagcagattc cgctgtgcaa cagttgatcg tttatcaagt atttgaagat
    12421 aaatatggag atgacgtatc aaccaaagaa atcgattctc aatatgatca aacaaagaaa
    12481 caattgggtg attcatttga ttcacaattg aaatctgcag gttacacaga acaaacattc
    12541 aaagacagta tcaaacaaag tctagccttt caagaaggtc taaagaaaca tatcaaattg
    12601 accgatgaag atttaaaaac ggcttgggaa tctttccatc cagaagtgga agctcaaatt
    12661 atccaagtag caagtgaaga tgatgcaaaa gatgtgaaga aagctgctga taagggagac
    12721 gatttctcta aattagccaa agacaagtca actgatacta cgacaaaaga agatggcgga
    12781 aaagtgaaat ttgattccac tactacaacg gttccggcag aagtcaaaga agcagccttt
    12841 aagttgaaag atggtcaagt ttctgatgtg atcacttcta ccaatgcttc aacttatact
    12901 acggaatatt atgtggttaa aatggtgaaa aaccaaaaca aaggcaacga catggataaa
    12961 tacaaaaaag aattgaaaga aattgcaaca gacacaaaat taagtgacag taccttccag
    13021 aataaagtaa ttggcgaagt attgaaagac gctaatgtaa aaatcaaaga caaagacttt
    13081 gaaaatgttt tgagtacatt cacttcagat tctagtacag caagtagtaa ataagcggta
    13141 taaataaaaa atatgaacta tttaaatcaa taaaactgtt catct
//
LOCUS       DACDTO010000084        13185 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDTO010000084 DACDTO010000000
VERSION     DACDTO010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.084"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            74..1813
                     /gene="yfcC"
                     /locus_tag="IWK70_002300"
     CDS             74..1813
                     /gene="yfcC"
                     /locus_tag="IWK70_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9614805.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            1970..3154
                     /gene="iadA"
                     /locus_tag="IWK70_002301"
     CDS             1970..3154
                     /gene="iadA"
                     /locus_tag="IWK70_002301"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9614806.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            3265..3468
                     /locus_tag="IWK70_002302"
     CDS             3265..3468
                     /locus_tag="IWK70_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9614807.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(3569..4231)
                     /locus_tag="IWK70_002303"
     CDS             complement(3569..4231)
                     /locus_tag="IWK70_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9614808.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(4244..5746)
                     /locus_tag="IWK70_002304"
     CDS             complement(4244..5746)
                     /locus_tag="IWK70_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9614809.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            6294..7973
                     /locus_tag="IWK70_002305"
     CDS             6294..7973
                     /locus_tag="IWK70_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9614810.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            8100..8579
                     /locus_tag="IWK70_002306"
     CDS             8100..8579
                     /locus_tag="IWK70_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9614811.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            8798..9478
                     /locus_tag="IWK70_002307"
     CDS             8798..9478
                     /locus_tag="IWK70_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9614812.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            9486..10817
                     /locus_tag="IWK70_002308"
     CDS             9486..10817
                     /locus_tag="IWK70_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9614813.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            10832..11794
                     /locus_tag="IWK70_002309"
     CDS             10832..11794
                     /locus_tag="IWK70_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9614814.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            11905..12243
                     /locus_tag="IWK70_002310"
     CDS             11905..12243
                     /locus_tag="IWK70_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9614815.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            12322..13029
                     /locus_tag="IWK70_002311"
     CDS             12322..13029
                     /locus_tag="IWK70_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9614816.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4185 a         2332 c         2897 g         3771 t
ORIGIN      
        1 ttactgtata attcatttga ttatccattt tttattcatc aaaattactg aaaaaagaaa
       61 ggaagaaata aatttgaaga aaacaaattc ttcaggaaaa ccgaaacgcg acttcactaa
      121 attatcgaca ccccatacgt atgtcattat ttttggtgta gtcatctttg catggatact
      181 gacttttgtc gttccagcag gaaagttcag cacgcaagat attgaataca aagatgcaaa
      241 tggcgaaaca agtactcgta ctgttttacg tcaagattcc ttccgttatg cttacgagct
      301 ggataagtcg tatgtgtttg atcaattaga agaactgcaa gatcatccag cagaacgtga
      361 aaaactggat gttccggaaa aaggattgga aaaagtcatt gctgacggag aaaaaaatct
      421 tacgcaggaa aaactagatg aaatctcctt gacagatgat gtgctttacg atcagtacgg
      481 cgaaaatatc tatgatactt ctaaaaaatt gcataaaaca gccaaaatat ggggaacaga
      541 tgattttggc ggctttggtt tcttaaattt tgtttttgaa ggactagtat ctggagataa
      601 gtatggatct gctgtaggga tcgctgcatt gatccttgtt gttggaggtg cttttggtat
      661 tattatgcgt accggagcga tcgacgcagg tatctatgcg tttatcagta aaactaaggg
      721 actggagcgt ttagctttac cgctattatt ctttgctttt tcattcggtg gcgctacatt
      781 cgggatggca gaagaggtaa ttccattctc aatggtgatg gttccatttg tcattgcgtt
      841 aggatatgac tcgatcgtag cagtaaccgt gacttatgtc gcatctcaag tcggaaatgc
      901 gacctcttgg atgagcccgt ttagcgtagc ggtggctcaa gggattgctg gaatccccgt
      961 gttgtctggt gcgacattcc gattgattat gtgggtagtt gttactgcat tagccgctgg
     1021 ttatatgatg atatatgcag aaaaaatccg taaaaaacca gaaagttcgt tgacttacaa
     1081 atcagatgat tatttccgtt ctcatatcaa aaagacatct gatgaaaata aaaaattcat
     1141 gcttgggcat aaattgatct tattagagat gttagttgtc cttgtctgga tcgtttgggg
     1201 cgtgactcaa aaaggctatt acattccaga aatcgcttct caattctttg tgatgggatt
     1261 agttgctgga atcattgctg ttcttttcaa attagatgga atgaaagtca atgacatcgc
     1321 ttcttctttc caatctggtg cagctgattt agctggtaca gcaatcgtag taggtatggc
     1381 caaagggatt cttcttgtct tgggaggatc tgatgcatct gttccttcag ctttgaatac
     1441 gattttgcat gggatcggga cggcgttgac cggtgttcca gctgtcattg gtgcttgggc
     1501 gatgtacatc ttccaaagtt tgttcaactt ggttgttaca tcaaattcag gacaagcagc
     1561 tttgactatg ccaatcatgg ctccactggc agatttagta ggtgtatcca gacaagtagc
     1621 agttcttgct tatcagcttg gttcaggttt tatggacgct tttacacccg tatcagccag
     1681 cttgattggt gtgctaggtg ttgcacggat cgaatgggca aaatgggcaa gattccaaat
     1741 caagatgcaa ggattcctgt ttgttttagg aactattttc atcatgattg ccattgcaat
     1801 aggtctgcag tagtactgga gacctgtaaa taaaaaagta atccaaacag tagataagga
     1861 ctccaagaac cgtcatttta cggtaaaatc aaaggagtaa agatcctatc gtttggatta
     1921 ttttttaaga tcctatcatc agttatactt aaagaaagag gaaaaatgta tgaaaatcat
     1981 tcgacaaatt gaagtgtttg caccagatta cttaggaaaa atggatgtac tgattgccgg
     2041 tgggaagatt ttggctgtag aagaacagct agaaggcggc tacgaaggag tggaagtaga
     2101 agaactttca ggtgagggaa aagtactaac cccgggtttc attgactgtc atttccatat
     2161 tttaggtgga ggaggagaag gcggttatca aaatcgaacg ccagaagtca cattgagcca
     2221 attgactaca gctggtgtaa cgacagttgt cggttgtttg ggaacagacg gagaaggtag
     2281 agatatgact gcgttgatca gcaaagcgaa aggtctggaa gcagaaggaa tctccactta
     2341 tgtatacgaa ggttcgtacc gattgccggt aaaaacagta acagattcta tcatcaaaga
     2401 ctttttgaca attgataaaa ttatcggaat cggagaaatc gcagtatccg atcaccgttc
     2461 ttctcagcca agttttgaag agttcgctcg tgcagtagca gatgctcgtg taggaggcat
     2521 gttatccgga aaagctggga tcatcaatgt ccacttaggc ggaggaaaaa gaaagctaga
     2581 gctattgaca cgaatcgtag aagaaacaga aattccgatc acacaatttt tgcctactca
     2641 tgcaaatcgc acaccagaat tatttgaagc ttgcgtggca tttgccaaac gtggtggcac
     2701 gattgacttc actgctagcg aagatcctga tttttgggag aagacagatg gtgaagttcg
     2761 cttcagcaaa gcactgaaac gattgatcga ggaagatgtt tctttagata atttcacgat
     2821 gacctcagat ggacaaggaa gcttgcctta ttttgatgaa aacaatcatt tcctagggtt
     2881 aggagtagga agtgccaagg cattgctagt agggatcaaa gaagctgtgc aaaaagaaag
     2941 tatccctttg gaaatcgcct tacgagctat aaccagtaat ccagcacgta tattgaaatt
     3001 ggataaaaaa ggaaaaatcg aaattggtgc tgatgcagat ctgtgtatct tggataaaga
     3061 aacgcttgat attgatacag ttatcgctaa aggagaaata atggtccaag aaaaagaagt
     3121 caaagtatgg gggactttcg aaaaaagttt ttaatcaaca gctctaacta tcttttttag
     3181 aaaacgagcg ttctgtttgt ctgtatctgt tgttgatgtt accctgaagg tagatcaata
     3241 aacaaactaa gaggaggaag cacaatggca gatttaaaag gacgttctaa agatatgaaa
     3301 gataaagtag tcggtgaagg aaaagaagca tacggcaaag caacagacga caagagcaaa
     3361 gaaacagaag ggaaagcaca atcactaggt gctgacatca aagaaaaagc tcgaaattta
     3421 ggcgacgaag tcaaagacaa gttttctcga aaagacgaaa aaaaataaaa accacttatt
     3481 actaattgaa tcaagcaaat aaaaaatccg aaccatatct gcgagacaga tatggttcgg
     3541 attttttatt tgatcattat cctgtttttt aaacttcgat tttttgaaat tttttatttt
     3601 ttagttcaaa gacttcatca cagctttttg ctacttcaat ctcatgagtc acgatgataa
     3661 tacagcgatt ttgttcatgg gcgatttctt ggaaaagttt gacgatatct tgtgaagttg
     3721 tttcatctag atttccagta ggctcatccg ctacgattag ttgatgttca cagcagatag
     3781 cacgaacgat agcgactcgc tgctgctgac ctcccgaaag tttggtcacc tgtttttttg
     3841 ctaatatttc atcaattcct acacgagcca gtcgatctaa agcaacttgt cgataattct
     3901 cttgcttggt cttagtgatt tccattgctg ctatgacgtt gtctagagcc gataaataag
     3961 gaagcaagtt gtaagcttgg aaaataatcg agacatcatt tttacgataa cttcttaacc
     4021 cgattttgtt cagtgaacga tcttgatagt atacctttcc ttcttttggt gcatctagtc
     4081 cagaaataag cgaaagaaaa gtcgtttttc cagaaccact tgctcctaaa atagcgtaca
     4141 tttttccagc ttcgaaattc agttctacat ctttataaag cgcatcttgc ggatcttgat
     4201 accaaaatcc caattgttct gtccgtaaca tcaaattcct cctttagttt aataggattt
     4261 ttcttgggtt taatcttaat atccctatag aagctaaaaa gacagaaaac agactgattc
     4321 caaatgctac tccggctaat agtcccaatt gtgcaggttt gacattgatt tccaattcgc
     4381 tgacttgatc agaaacagca aatggattat tcgtggttcc ttgttgacca tcgcgttgac
     4441 cgccttgttc ttgtctgtta cctggcattt gtccaccgcc aggagcttgc tggttttgtg
     4501 tcgtggtttc tgtctgttgg tcaagtagct gctgtcccac ggcattcgcg acgacgtttc
     4561 cgctaagcga agcaactcct aatgcaagaa tcatacaaat agccacttcg gtaaacaatt
     4621 ggagaatgat tttacttctg gattctccta aagatagcaa gacgcctaac tcatgccgac
     4681 gctcccgaat ggtaatcata atgatcagag taagtatgat aataccggct acagccacta
     4741 gtaaaacaac atttttagaa aaacttgcca cgttgtttaa aggttccaac attgactgat
     4801 acatggaatc atttgattgt aagctaaatg tatctgtgtc gatcagcttc tcagcttctt
     4861 tgagaaactc atccatttta tctggatcag atagagtgta aatagcagaa tctatcgtgt
     4921 tatcgtcaga tgtcccattt agttcatttg caaaagtgta agaagtgtaa agtgtattcg
     4981 acggattcat gaagttgaat ttcataccca tactgccgct tgtttcgctg gattcataaa
     5041 ttcctttgat cgtcatttca taagtggtat cttcatcttc agtactcgtg attgtgaaag
     5101 tatctccgac tgccaggtca tttgtttctg ctagagtaga atcaatgact acattgtttg
     5161 tgcctttgtc gctgtcatct attccttcac catccgtgat ttttgctgtt ccatcagaaa
     5221 aattactatt ttgtgcagtt gctgacacac cactgatttg aaagtctgct tgagtcattt
     5281 gcggattccc gcccattcct ccagcaggtt caccgcctgc atcagtagtt tctgaactgt
     5341 cttcacttga atcagaacta gtgatggcag ttattccatc ttttgccata gctgaggcgg
     5401 aactttcaaa ggaataactc tttacattat ctaattcagc tattttttcc gcgtccgtta
     5461 aggaaactgg tgtaagagaa aaactgcctg gatctggacg ttcttcagtg gtagaaccgc
     5521 ttgaagaagt ttgttcctgt tttttaaatg aagcttctcg attggcactt aaagtcactg
     5581 ttgctccaac ttctttttgt gcttgattcg ctgcttcttc tgcagcgctt cgaatcgtga
     5641 ggccagctag cacaaaaata agaatggctg tgaacacggc aaacaataac aatgaacgtc
     5701 cccattttac ttttgtactt ttccatgcac gtttcataaa attcatattt tgcatccctc
     5761 ctagtagatg catttgataa tagacaacct atcttaaagc tagcttaaat cttatgctgt
     5821 ttgcacacca tcaacatgag gttctgtata tataaaaagt cactatagtt attgcaaaaa
     5881 atgtggtaaa atgaaaaaag tatgtttcaa accgacgaag aagaaatgaa agaggaatag
     5941 caaaagaaat cctagatgct acagagggat ttttacgtgc atagttgttc taacaattgc
     6001 tgatactagc gatttagttt atttgttgta taataatggg gaacagctga atacttatat
     6061 aagtattctg gctatagcgt ttcgcaaaag atccattttg ttaatgaagt atatccaaaa
     6121 taaaatgccc atccaactgt cagaacgcgg atgtgctggt aattttcctg ctgattctcc
     6181 ataagttgag aaatagtgat tttgcatatg cttcattgga atggctcata tatttagata
     6241 aagaagaaaa tcgacatctt taaacttgga tgtcataaga agaggtgaag tttgtgagta
     6301 ccattaaaat tatcccatta ggcggcgttc gtgaaaatgg aaaaaacatg tatgtcgcag
     6361 aagtgggcga ggatattttt attttagatt gtgggctaaa atatcctgaa aatgagttgt
     6421 tggggatcga cgtcgtaatc cctgatttta cgtatttaga agaaaacatt gatcgtgtag
     6481 ctggtgtctt tttaacacat ggacatgcag atgcgatcgg ggcacttcct tacttgttgt
     6541 caaaaatttc tgtaccagta ttcgggacag agttgacgat cgagttagca aaattgaatg
     6601 tcagtcgtaa tgaagcagcc aaaggattta aggatttcca tgtggtagat gagcatacag
     6661 aaatagactt tggtgaaaca gtagtgagct ttttccgtac cactcatacg ataccagatt
     6721 cagtggggat caatctgaaa acaaaagaag gtagtatcgt ttatacggga gattttaaat
     6781 ttgaccaaac ggctatccca atgtatcaaa cagattatgc acgattggcg gaaatcggca
     6841 aagaaggagt cttagcctta ctaagtgaat cggcaaatgc agaaaatcct cagcctgtag
     6901 cttctgaagc ccaaatagca gatgaagtat ttgatacgat caaatattgg gaagggcgta
     6961 tcatcgtcgc ttgtgtagca agtaatttgc aacgagtgca acaagtattg gatgcagcag
     7021 aaaaagcaca acgaaaagtt gtcttgactg gtcaagattt cgaacgaatt attcgcacag
     7081 cgatgcgttt agaaaaactt caattaccaa gtgaagatct gcttgttacg ttgaaagaca
     7141 tgaaaaacta tgatccagaa gaactgatca ttttagaaac tggccgaatg ggtgaaccca
     7201 tcaagtctct gcaaaaaatg gcaaacggta cacaccgtaa tgtcaagatt gaagaaggag
     7261 atttggttta tatcactact acgccaagca tcgcgatgga aaccatcgtg gcgaaaacgg
     7321 aagatatcat ttatcgagct ggtggaacag tgaagttgat ctctgagaat atgcgcgttt
     7381 ctggccatgc caatcctaat gacttgcaat taatgatcaa tttgatgaaa ccaacatatt
     7441 ttgtgccagt tcaaggagag tatcgtgaat tagctgctca tgcagatttg gctcatgcag
     7501 tagggatgcc atacaaaaat atctatatta ctggacgcgg agacgtgctg gaatataaga
     7561 acaagcgtat gagtgttgca ggaactacaa gtgcagaaaa tgtaatgatt gatggattag
     7621 gcataggtga tatcggaaac attgttttac gtgatcgaaa aatcttgtca gaagacggga
     7681 ttttcgtagc ggttgtcact atcagtcggc gagaaaagaa aatcatttca aaacctcaaa
     7741 ttacttcacg gggattcgtt tacgtaaaag ctagccgtga tctgatgaaa gaaagcagca
     7801 atatgattga aacaatcgtt gagaaacatt tagaaagcaa tgattttgag tggagcaaat
     7861 taaagcaaga catccgagat caattaggcc gttacttatt tgaacaaaca aaacgccgcc
     7921 cagtgatctt gccagtcatt atggaagcta ctcaacgtcg aaataaaaaa taaacaaaaa
     7981 gagtaagatg aacctctcta tagagtgctc gtcttgctct ttttttgcga aaaacaaata
     8041 atttttgaat acagcatccc tcgctttata ataaacgata gaataaaggg agtgaatcaa
     8101 tgatgagtca aacaaaagtc gaacgttatt tagaagaaca aaagatcagc tatcaaccac
     8161 ttgattttag tcatgtggta gaagaatctt tcacagaaga actacaaaaa agagggattg
     8221 atccagcttt gatatgtaaa actctcgtag tgaaaggaaa caaaactggg gtgatcatcg
     8281 cattgattcc tttgaacgat cacttagatt acaaaaaaac gagacaagtg acgaaagatc
     8341 gtaaagttgg ttttcctgga atggattttg tattaatgca tacaggatat ccccacggag
     8401 caaacacccc tattggaata aaactcgcac atcctgatta ttgtttttta gcagacagtt
     8461 ctttaaaaga aaaagaagaa gtaattgtgt caagcggtga aatcgggaga agccttcgga
     8521 taaaagtagc cgatttagaa aagatcgtcc atcctattta cgctgattta attagttaag
     8581 ggacgagaag agaagctaag cagagcagag acctcaaact tgagattata tctgcttggt
     8641 acttggtgca tcctgtatta tctgttacaa ttgctttgta catactgtac tatcaatcca
     8701 atgaaaatat atttagcagc tatatttttt cattgatgtg caagtgatga aatcgttttt
     8761 aataaaaaag atacagagaa aacgcgaggt gtaaaatatg aaaattacgt atcatggaca
     8821 ttcctgttta acaattgaaa tggaaaatgg ccaacgcgtg atgattgacc cgtttattac
     8881 aggaaatcca ttgacagact taactgcaga agaagtaaag acagattggt tgctgattac
     8941 tcatggtcat agtgaccaca taggagatat gattcctatt gcaaaagaaa atcaagccac
     9001 aattatcagt attgttgaga ttgctgatta tgcgaacagc agaggagtag attcattcgg
     9061 tatgaatatc ggtggaaaac atgcgtttcc attcggcgtg gtaaaattcg tacatgcaca
     9121 gcatagttca agctatgaag tagacggtat cgttcagtat atgggcgagc ctagcggaat
     9181 tattattcaa gcagaaggaa aaacgattta tcatgcaggg gatactgctt atttcagtga
     9241 tctaggattg cttgcagaag aattcgatat tgatgtcgct ttcttgccga ttggcgataa
     9301 ctatacgatg gggccagaag atgcagcaaa ggctgcgcaa acaattcgtg ccaagaaagt
     9361 agttccaatc cattacaata ctttccctgt cattgaacaa gatcccgaat cttttgtaga
     9421 gttactggaa gatagtgaag gaaaaataat gagcgtctcg gaaacgctgg aagtgtaggc
     9481 agaaaatggc gacaaaacat gatttgattt tagaatatat tgaaagttta cctgttggca
     9541 atcgaatttc tgtaagaagc atcgcaaaag aattgaatgt cagtgaagga acagcgtacc
     9601 gtgcaatcaa agatgcagaa aatgtcggac tagtttcaac aattcaacga gtagggacca
     9661 tccgtatcga acgcaaatta aaaaaacata ttgaacgttt gacatttggc gaggtcgtca
     9721 ggatcattga aggggacgtt ttaggcgggg cggcaggatt agacaaggtc ttgaataaat
     9781 tcgtcatcgg cgcgatgact aaacatgcca tggaacgcta tattacgcca ggatcactaa
     9841 tgatcgtcgg caaccgggcg gaagtccaaa aattagcatt ggaagatggc gccgcagtat
     9901 tgatcacagg aggattcaat acgactgctg agattgcaaa gctagctgat gatcttgaaa
     9961 tgccgatctt acgcacctct tatgatacat ttacagtcgc aaccatgatc aaccgagcgt
    10021 taagcgacca attgatcaaa aaagatatca tgcttgtcag tgatatctat atgccagctg
    10081 aaaagactca ttatttgcag cagacagata cgatttctga ttatcagcgt ttgtcagaag
    10141 aaacgcagca ttcgcgtttt ccagtcgtca accgccatca tcgtttgatg gggattgtta
    10201 cggcaaaaga tgttttaggg aaatcgccaa accagatcat cgatcgggtg atgacgaaag
    10261 aaccaatcag tgtaaagaaa acaatgagta ttgcctctgt tagccatcaa atgatctggg
    10321 atgggctaga agtcatgccg gtcgtttccg atgacttgac attggaaggc ttgattaccc
    10381 ggcaagatgt aatgaaagcg atgcagcttg tccagcgaca accgcagatt gcagatacga
    10441 tttcggatca gatatctgga aatatccata cgattgatac tgaccgtgaa ggaaatcgtt
    10501 tggaaaaccc taagttcaaa ttcgtagtag caccacaaat ggtcaatggc gttggaacca
    10561 tttcttttgg cgtacttagc gaaatcattt ctgatgtcgc acaaaaaaca atggtgatga
    10621 accaaaaacg aaatatcttg attgaacaag tgaacctaca ttatttgcgg ctgatccaat
    10681 tggaaagcga attagacatt cgtccacgtg tattggaaat cggcagacgt tccgctaaac
    10741 tggatatcga agtgtttatt gaaaacacca tcgtttccaa agcaatcgtt gtttgtcaag
    10801 taatggaacg ttcttagaaa gcaggagtga aatgacagta cagcaagaaa tagtagatac
    10861 aataaaaaaa tacgataaaa tcattatcca caggcatttg cgccctgatc ctgatgcatt
    10921 aggatcacaa gtaggattag ccgaactatt acgggaaagt ttcccagaaa aagaaatacg
    10981 tcaagtaggt gaaacgattg aagggcttcg ctttttagca gaaatgcaag aagttgaaga
    11041 tgtcttctac caaggagcgc tagtcatcgt gactgataca gctaattcac ccagaatcag
    11101 tgatcaaaga tatcagttgg cagaaaaact gatcaaaatc gaccatcatc ccaatgatga
    11161 cccttatgga gatcttgtgt gggtaaatac aaaagcaagt agctgtagtg agatgattgc
    11221 agaatttgct ttgatgtttc ctgaatcttt aaagatgaac gttaaggctg cacggttact
    11281 ttatgcagga atcgtcggag atacagggcg ctttttgtat ccctccacga cgtcccgtac
    11341 attagagatt gcttcgatat tgagaaatta tccatttgat gcaagtgctt tgaacagaga
    11401 gattgaacaa atgccgatga aagtcgctaa actttccggt tatctttatc aaaatatcca
    11461 ggtggatgaa aatggtgcag gaagaataat ccttccgcaa gaaattcttg ataaatatgg
    11521 aatcgatgat tcagagacag cttcgatcgt ttcacttcct ggagtgattg acgaagtgct
    11581 ggcttggggg atttttgttc agcagccttc agggtattac cgcgtacgtt tacgttctaa
    11641 ggggccgatc atcaatgaat tggctaaacg ccatcatgga ggaggccatc cattagcaag
    11701 tggtgcgaat gcaaaagatt tagcagaagt agaggaaatc tataaagaga tccaagatat
    11761 atgccaatcg tataagagca gaaaaacaga atgatacttc gttcgaaaac gtgccttttg
    11821 tagtaaaaat cacaaatgta tcttttcaag atcttctttt tccgttacaa tagtgctcac
    11881 taaagatttt aaaggagaaa gaagatgaca ttaccaaatt gcccagaatg tggctcagag
    11941 tacacatatg aagatcgagg cttgttgatt tgcccagaat gcgggaacga atggaacaaa
    12001 gaagaacaag aaactgtcat agaagacgga ttagtggtat tagatgcaaa tggaaaccgt
    12061 ctgttagagg gcgatagcgt gacagtgatt aaagacttga aagtaaaagg tgcgtcaggt
    12121 gccattaaac aaggaacaaa agtcaagaac atccgtttag tagaaggcga tcataatatc
    12181 gattgcaagg tagaaggatt tggaccgatg aaactgaaat cggaatttgt gaaaaagaat
    12241 taaagaatat tcaaggtctt ttcacattct tttcctacta ctaatgataa aataaagtaa
    12301 acgaaaagga gtgaaacgaa aatgtatggt tatggaatgt atggtttttt agatccgaca
    12361 tttcttttgg tcattcttgg attagttatt tccggaattg cgtcagcgta cgtcaatagt
    12421 actttccgaa aatacgatca agtgagaagc aaaaacaatg tgactggtac acaagcagca
    12481 caatatatat tacaaagtca acagataaat aatgtcggtg ttcaacaaat tgccggagat
    12541 ctcacagata actacaattc aggtaataag atgttgagtt tgtctcaagc aactgcacag
    12601 tctacttcag ttgctgcgat aggtgtagca gctcatgaat gcggacatgc ggtacaagat
    12661 gcttcgggtt atgtcccatt gaagattcgt gcagcacttg ttccagcagc taactttggt
    12721 tcgatgattt cgttcccctt gattatggta ggtgtattgc ttagctggaa ctctacgtta
    12781 atcaatatcg ggattttcgc tttttcttta gcattacttt tccaattggt gacattacca
    12841 gtcgaattta acgcgtctcg gcgagcaatc caaattttga gtgaaggcgg actactgacg
    12901 gaagaagaag taccgatggc aagacacgta ttatttgcag cagcactgac ttatgttgca
    12961 gcagcgcttt cgacattcct gcagctgttg cgcttgatct tattgttcgg cggcaaccga
    13021 agagattaaa ataaagaagt tgtgaaatca gcgctttgat ctgagctaat accgagagtt
    13081 agctgattga ataccattta tttggaataa gggctctttg tcaataagga ctgatgggtt
    13141 gtacaaaatc aaatctgggt cagaatgaac ctcattctgg ctcag
//
LOCUS       DACDTO010000085        13022 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDTO010000085 DACDTO010000000
VERSION     DACDTO010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.085"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWK70_002312"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IWK70_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9614817.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWK70_002313"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IWK70_002313"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9614818.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IWK70_002314"
     CDS             complement(2169..2906)
                     /locus_tag="IWK70_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9614819.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IWK70_002315"
     CDS             complement(2997..3524)
                     /locus_tag="IWK70_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9614820.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IWK70_002316"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IWK70_002316"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9614821.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IWK70_002317"
     CDS             complement(4401..4886)
                     /locus_tag="IWK70_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9614822.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IWK70_002318"
     CDS             complement(5039..5236)
                     /locus_tag="IWK70_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614823.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWK70_002319"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IWK70_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9614824.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWK70_002320"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IWK70_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9614825.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IWK70_002321"
     CDS             9211..9423
                     /locus_tag="IWK70_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9614826.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IWK70_002322"
     CDS             9425..11104
                     /locus_tag="IWK70_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9614827.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(11358..11558)
                     /locus_tag="IWK70_002323"
     CDS             complement(11358..11558)
                     /locus_tag="IWK70_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9614828.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(11708..11851)
                     /locus_tag="IWK70_002324"
                     /pseudo
     CDS             complement(11708..11851)
                     /locus_tag="IWK70_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            complement(12086..12748)
                     /locus_tag="IWK70_002325"
     CDS             complement(12086..12748)
                     /locus_tag="IWK70_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9614829.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
BASE COUNT         3815 a         2789 c         2216 g         4202 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta tttttgttac tcgctttgtt ctctcgtaaa aattgatgtc taccatcaat
    11341 ttgatttaca gataaaatta agcttttaca acgtttgcag cttgtgggcc gcggtctgat
    11401 tcttcaacgt cgaaagtcac tgcttgacct tcttctaatg ttttgaatcc gtcaccttgg
    11461 atagctgaga agtgaacgaa tacgtcgctt ccatcttcac gtgagataaa tccaaatcct
    11521 ttttcagcgt tgaaccattt tactgttccg ttttccataa taatatcctc ctcgtgccaa
    11581 aagcactaat ttaaattgta acattgctcg tgtacgatag aggtgcaaca gatgtttcaa
    11641 cttctattcc caaacaaaat tacaatataa ttgtacactt gcttcatttt ttttgcaaga
    11701 aagcaactta cttctcttcc atcattcctt cttctctcaa actaaaatat cgtttgcgtc
    11761 cgatgatcaa gtggtccagt acagtgatcc ccatcatttc tccacacttt tgtatccttt
    11821 tggtaaaatt catatcctgc tgcgatggaa tttcatatta tcaagagtaa attacaaaag
    11881 aatcatttaa atcaatactt tcactttgaa tacatgtatt tgaagtggaa ggtacttatt
    11941 tcaaaaatag taaaacctgt atcttaaatt acttaatagt aacataagat acaggctgat
    12001 tttttattca ttgttattta tactaaagca cccgatagct ctgaaaacaa tcacaaattc
    12061 aacttttcaa agccacagct ttaagttatt ttgtccagac aacccccatt gcccaaccca
    12121 ttttatggaa ttggcatcca ggcaacaact tttcatataa atcgtaataa ttttgttcag
    12181 ataggtactt atctgaagct aaatgctcaa gccatgattt agatgtgttg tgattataaa
    12241 ttctaatcgc attttttatt ccaaatttga aacaatgcgg taaaaactct tggattgccc
    12301 ctaatttata aacataggta ggcggtgtct cgacttcaga tacattatca agaataacta
    12361 ttcttccttc ctcattcaac agttccttca tttgctgtat tacgctggct atatcatcca
    12421 aatgatgaaa ggttgttcgg cttacaataa aatcaaactt ctcattaaaa ttaagttgtt
    12481 ctgcattcat attcagatag accgtatttg ttagttgacg tttagatttg gcaagatcga
    12541 gcatttgatt agaaatatca atccctacca cttcatcata ataacttgct aatttctcca
    12601 ctaacaaacc cgagccacat ccgatatcta atgctctgcc tttctttgga gacatattag
    12661 acacaaagaa tgaataatca ttcaaaagct cattcacgaa atcgtaatct tctgcaacct
    12721 tatcaaactg tgattctatt gtattcaaaa agatccccca ttcctacttt atcgacattc
    12781 tttcattact taccacttta gatgtttttt cgttggggat aaaacttccc tttagacaat
    12841 tttatccaaa gacaatacaa cagtgcaact ttattaaagt cactgtcctt tatcgcagcc
    12901 tttacttttt agtaaagaca gtggcttctc ttatcaagtt tcaaaacata ttattttgaa
    12961 gaaaacgtcc atctgaagtg tcaagtgcaa aattacatat aaaggtttat tctaaaatga
    13021 aa
//
LOCUS       DACDTO010000086        12734 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDTO010000086 DACDTO010000000
VERSION     DACDTO010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.086"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWK70_002326"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="IWK70_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9614830.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="IWK70_002327"
     CDS             complement(322..891)
                     /locus_tag="IWK70_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9614831.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="IWK70_002328"
     CDS             1077..1979
                     /locus_tag="IWK70_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9614832.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWK70_002329"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="IWK70_002329"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9614833.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWK70_002330"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="IWK70_002330"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9614834.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWK70_002331"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="IWK70_002331"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9614835.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="IWK70_002332"
     CDS             8547..8732
                     /locus_tag="IWK70_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9614836.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="IWK70_002333"
     CDS             complement(8794..9990)
                     /locus_tag="IWK70_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9614837.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="IWK70_002334"
     CDS             complement(10160..11596)
                     /locus_tag="IWK70_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9614838.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="IWK70_002335"
     CDS             11889..12698
                     /locus_tag="IWK70_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9614839.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DACDTO010000087        12104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDTO010000087 DACDTO010000000
VERSION     DACDTO010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.087"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            474..725
                     /locus_tag="IWK70_002336"
     CDS             474..725
                     /locus_tag="IWK70_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614840.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
     gene            759..1037
                     /locus_tag="IWK70_002337"
     CDS             759..1037
                     /locus_tag="IWK70_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614841.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            1634..1891
                     /locus_tag="IWK70_002338"
     CDS             1634..1891
                     /locus_tag="IWK70_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614842.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            2004..2615
                     /locus_tag="IWK70_002339"
     CDS             2004..2615
                     /locus_tag="IWK70_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614843.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            2794..3351
                     /locus_tag="IWK70_002340"
     CDS             2794..3351
                     /locus_tag="IWK70_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614844.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(4050..4571)
                     /locus_tag="IWK70_002341"
     CDS             complement(4050..4571)
                     /locus_tag="IWK70_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9614845.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            4770..8462
                     /gene="nifJ"
                     /locus_tag="IWK70_002342"
     CDS             4770..8462
                     /gene="nifJ"
                     /locus_tag="IWK70_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9614846.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            8651..9538
                     /locus_tag="IWK70_002343"
     CDS             8651..9538
                     /locus_tag="IWK70_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9614847.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            9535..10704
                     /locus_tag="IWK70_002344"
     CDS             9535..10704
                     /locus_tag="IWK70_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614848.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            10728..12083
                     /locus_tag="IWK70_002345"
     CDS             10728..12083
                     /locus_tag="IWK70_002345"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9614849.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
BASE COUNT         4031 a         2031 c         2657 g         3385 t
ORIGIN      
        1 acaaataatt ggtttattct ggcaggcgta atgctttttt ctagtttcta tttatttagt
       61 aatgctctat tagcacagcg agggaaatat aagtttaaaa tattagatta gatattattt
      121 ttagagggga taaatataaa atgaaaagag gttatttata atggaaaaag tattaccaat
      181 taagagctac tgtattccaa ggcttttcat catagaattt caaaagtaaa gacatgtgtt
      241 tcacaaatag tgtctatcct ggctaaataa ttcttccact atctgcttta atttcagtag
      301 acttttcggc aagttatttg ctaatgagat gaatagagat tacaactcgc ctataaccgc
      361 ttaaaacaag cttttttaaa gtatagttag caagtcgtat tcatatgttc aaagaggagg
      421 gcttatgaac tttgaattca ttccgtgtga acagaacagt agggggaagt aaaatgaaag
      481 aaaagcctat aaataaatgg tcattgtata aaaattcaat gtttggagtt gttatctttg
      541 gtgtttgtgc tggattattc cttttcatat tattacaaac ggtgactctt ttctcttcta
      601 atcctactta tggatggaga gacagtatag ccttagggat gtttgcggga ggatatggat
      661 tcttattgtt gttctcaatt ctcacactaa gtccaatcca tggactcatt caactttgga
      721 agtaagaacg agccttaaac ttttcgttct cggaagaaat ggaaaaacac aacataacag
      781 gcagtatgta tgtggatgat caatggttta tcagcactga tgaggtaaaa atagctgcca
      841 ttaggagaga ttacctgcag aaaattcata aaataaaaga tcctaatggg aaaaaggcaa
      901 ggttaattat aactttgata acatctaatg gtaagaagtt aaaacttgca ggatcatatg
      961 ataatgttaa aaagttcgaa cattggtatc tgcaaaaaga cagcattagt cttaataaac
     1021 aatttgaaaa aaattaatca aagtcatcgg caacacgaat aatttgacat cttgatattg
     1081 tgatataagc aaaaacggca tttctgagac cgaacagcat aaaaaattta aatgttggaa
     1141 tttatataag caaaacccaa gaagctagat gcgatagagc atttaatttc ttggggtttt
     1201 gcttgggaat cagcatatca attaaaatga tcatctcggt atttcaaaag tgggtacgat
     1261 tacaaatagt tggagcaaaa tagtgggtaa aattaccatt ccagtgtctg tcttgcctct
     1321 ttttcgtcaa tcacaaatag acgagattca taggaaaaat cgatacaggg tatgatttca
     1381 atgatattat gaccaattac aaaatagatt gataaagcat tttttgcatt tttataatag
     1441 tttattgata ataatgtatg agatacagta taatgattta aaaggtaacg tgttgtgacc
     1501 gaattaaatg aattaatgcc tattttttat attccttagt ggacaaaaat ggagacagtg
     1561 aagttaggag cattgtatcc tttgttgcgt agttcaaaaa agtaagcgat ttctataatg
     1621 gtcagggtaa acggtgaggg ctgtccacgt aaatcctact attgaaatca caatgatcgg
     1681 acattcaaaa ggtggtgctg aagcagcaaa ttcagtagct ataaatacga atgcgattat
     1741 atttaatcca gcgacagtaa atttatctgc ttatgaatta gactcaaaca catatacagc
     1801 aaacatggta gcttatgttg tcgagggaga tgcattaaat gttgtttttg gaggagtata
     1861 cacctattgg agaattaatc atgttagata gacaatatga tgcaccttag tggagaatga
     1921 tatctcgcat aggtaactca gtaaaaaatc aaggtatgga agcaattatc tctgcattgg
     1981 aagatcgata tagataggcg gatatgaata gtaatagggg gaataaaatg gaatacaatg
     2041 gatcaatgag aattgtacaa attatatttt ggattacatc gggaattgtg atcataggag
     2101 gagttttttt gatgttgcca agcttaatat ttcctttctt tgcattaatc tcacctaaaa
     2161 tacccgaacc ggaaatcacc tatggagaat ttccatttag gattgaatac gaactagatg
     2221 gacagattaa cataattgaa gatactgtca tagctgagtt taacgggtgt gagttcagcg
     2281 caggttctat gaaaagagaa cgaagatgga gaagtagatt agctagtggt agagaagatc
     2341 taccttttgg ggacgatcta ggaatctatt ttagtcgagg aagtgcgcaa tattatatgg
     2401 gggaaaatgt acaatcaatg tcattaaaac cacatatagc ccttagaaat ctcgaggaag
     2461 gttttaaaag agaggtagac tttatattag gagaaagtgg atacataaga actgtactta
     2521 atttcggaga agcgcaagaa gttttgacac aatacggaat taccttaatc aactgggaaa
     2581 tatctgaacc gattgttaac aattttggtg attagaaaac aattacgata aatagaaaca
     2641 agccaaatca tcagcaagta aaagctgatg atttggcctt tcttatacga gtagtaggaa
     2701 ttcatagcga atattattac gaccagtaga tattttgata ttaaaggagt attacgaact
     2761 aatatgtgag aatgtagagg tgatgtgcga ataatgagaa atttatcaaa tagaaacaaa
     2821 atattaatca ttattgttgt tatagctgtg tttcatctgg gaacgaatgc ggttttaagt
     2881 agaataatat tgggtcctaa gccaccaaga ccagaaatta cccgagggga gtttgatttt
     2941 agattagaat atgaagtaga tggggaaagg attgtgatag aagatacaat tgttgcatta
     3001 tttgatggat tttctgcaga tgcaggtagc atggcatggt atcgcacgtg gcgtctacat
     3061 ttagcaagtg atagacgatc acgaaatatc ttattagatg aattagagga tggtcgaaga
     3121 atatattatg tcccagaaag tgctaactat tctatgggag atgtacagaa aaaaagagag
     3181 cctaatccta attggtatcc atttaatggc gtaacaatag aatatccacg caataaaaca
     3241 cctgaaatag gtgcaaaatt tataagtggt ttagaagatc tgtacgatag gtttggaatc
     3301 cgtcttatta gttgggaaca cgatccacca atagaaaatc gctttgaata atcgaaatct
     3361 actaatccat ttcatcagcc agtgaatgtt gtaatggaac aatttggttt agtgttcaaa
     3421 accattgaaa tactaggctg tgatcagcaa aaatgaatct gaataaaaaa gagagcaact
     3481 attagctgag aggagttaat agttgttagc tttatacgaa tcaaagtggc aaatacagat
     3541 gcgtatgcat ttgtgtttgc catcttgcgt ttggtagacg agataggtat aagctaaaga
     3601 aacaacttgt ttaaaataaa cgacctagct caacaaatga ccatctaatc aactaacagc
     3661 atacataaaa gcaactattt ttgaagtaga attgaaatcg ggataactta aaaagtgatt
     3721 gggatactag tgagaatcac cgtacataaa tattactcgt taagggaaat tgagaaattt
     3781 tgtatgaaaa tttaagaatt agttagtatg cgcaagtcaa aaatatgaat aaattatttg
     3841 gtggagataa tttgcttagc ttgtcataag aaatgagata atctggcaga tgcaagcttt
     3901 tggacaccat ttattcggaa taaggaggct gggacagaag cgtttaactc caagaaataa
     3961 gaaggagttg cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc
     4021 tttacttttg tcccacgctc cttatttttt tattttgttt ggatcaattc ttttaattcc
     4081 tctattgaaa tagattcatc aaagatttct ttacccaaga tgacagttgg aacaaatcga
     4141 atatgtgctt gctcagcttc gttgatgaca gcttggctca gctgttcgtc tttttgttct
     4201 gtcagcttta gtttatctac tgcaaaatcc gctacttctt ggagagaaag atgtttccac
     4261 tcgtcttgag tatcgaatat ttgtttgatc tctttgatcg ctttttggcc atcgctgatt
     4321 gtgagatacc ggtgcatcac gttccctcgc tgtaagcttt ctttttcttt atcgaacaat
     4381 ttgatcactc gctgcaattg cccgctttgg actgcttctt ctaatagctc ataagattct
     4441 tcaaaccatt gtcggcaata cggacaagct aagttgatga attcgaccat ttttttagga
     4501 gcattcgatg aaccataaag gattcctttt tgtgtatttg ttttcgttgc atcaatcact
     4561 gatatatcca tgttaaaaca cctctttcgc ttatagttta tacatgtctg acaaaaaaag
     4621 gcaacgattt agactgtttg tctaaaaatt agatgaccaa ttcaaataga tacatgtaaa
     4681 atatttttat aaataaaaga aaaaaatggc atgttttacg ttataatgga acagataata
     4741 agagagaata tgaacaagga tgatgaatca tgcgaaaaat gaaaacaatg gatggcaata
     4801 cggcagccgc atatatttct tatgcattta ccgagattgc tgcaatatat ccaataacac
     4861 ccagttcgac catggctgaa ttagtagatc aatggtcagc agcaggaaaa gaaaacgtat
     4921 tcggtcaacc agtaaaagta gtagaaatgc aatccgaagc aggagcagct ggtgtggtcc
     4981 atggatcatt gaaaactggt gcactgacaa ctacatatac cgcttcacaa gggctgcttt
     5041 tgatgatacc gaatatgtac aagattgctg gggaactact gccaagtgtt ttccacgttg
     5101 ctagtcgggc agtgacgaca aatgcgttga atattttcgg agatcacgga gacgtgatgg
     5161 ctgcaagaca aacaggattt gctatgttag ctgaaagcag tgtacaagaa gtgatggatt
     5221 tagcacctgt tgcccatctt gcttctattg aaggagaagt cccttttttg aatttctttg
     5281 atggattccg tacgagtcac gagattcaaa aaatcgaagt tttggaatat gaagaattag
     5341 ctcctctgat caatcaggag aaattagctt catttcgtaa acgagggatg aatcccaatc
     5401 atccgactgt tagtggtacg aaccagaatc cagacatcca ttttcaacaa cgggaaacga
     5461 tcaaccccta ttatgataaa ttacctgaaa tcgttcaaaa atatatgaaa gagatcaacc
     5521 gattacgagg aacttcttat gacttggtga cttattacgg agcagacgat gcggaagaag
     5581 tcattgtttc tatgggatca gttgcacaga cgattgaaca aacgattgat tacttgaatg
     5641 caaacggacg gaaaacaggt tttttgaata tccatcttta tcgtcctttt ccagtggaaa
     5701 cattcgtgca aaagttgcct catacagtaa aaacaattgc tgttcttgat cgaagtaaag
     5761 aaccaggggc aggtggagaa cctctcttat tagacgttca aagtgcgtta tatgattctg
     5821 aacttcgccc ggctgttatt ggtggtcgct acggattggg gtctaaagat gtgactcccg
     5881 accaaatctc agcagttttt gatgaattga aaaaagaccc aagcatacgc aaaaaacggt
     5941 ttaccatagg gatcgtggat gatgtgactt atcaatcttt ggaaccaaag gaaagtctgg
     6001 atttgacaga accgcaaaca ttccaggcca agttttgggg atttggatct gacggaacag
     6061 ttggagcaaa taaatcagct atcaagatta ttggagatca caccgataaa tatgcgcaag
     6121 gctattttta ttatgattcg aaaaaatctg gcggtctgac tgtctctcat cttcgttttg
     6181 gagatacgcc gatacgttca gcgtatcttg tcgagcatgc ggaccttgtt gcctgtcaca
     6241 caccagctta cctgcatagc tatgacttgg tcaaaggatt gaagccaggt ggcatttttt
     6301 tattgaatac tctttggagc gacgaacaat tagaaacgca tctgccctta aaattaaaac
     6361 gttacttggc tgaaaataac attagattct atacgatcaa tgccatgaga ttggcccaag
     6421 aagtcggttt gggacgccgg atcaatacgg caatggaaac ggcttttttt aaattagctg
     6481 acatcattcc ttttgatgag gtgcttccgc tgctaaaaga agaagccctg aaaagctatg
     6541 gacataaatc aatggctatc gtagaaaaaa acatacaagc aatcgatcgg acgaccgaac
     6601 tgcttcatca agtcgcgatc ccagaccatt ggagaacgtt ggagatgcct gaaaaaatca
     6661 ataaaaggaa cactggttat atacaagaaa tacttgaacc agtgaatgca caggaaggaa
     6721 atcaactctc tgttggtaca cttgtagcaa atggaatgac aactggcgaa atgcctttag
     6781 gaacggcttc tatggaaaag cggggcgtcg ctttagaagt acctgagtgg attagtgatc
     6841 gctgtacgat gtgtaatgaa tgtgcctttg tttgtcctca tgctgccatc cgtccatttt
     6901 tagcagatga agaggaaatg gaagaagcgc cagaaggatt tatcgtgaga gaaatgcgag
     6961 gcgcagacgg cgtaaaatac cgtatccaag tttcagtgga agactgtaca ggttgtggtc
     7021 tgtgtgtaga ggcttgtccc gcaaaagaaa aagctttagt gatgaagcct tatgaagaac
     7081 agaaagaaca agctgtgaat tgggcttttg caatgacatt aagacaaaaa gcaaatccag
     7141 tcaaaggaaa gaatactgtc ttgtctacac aattcaatca accgcttctt gaattctcgg
     7201 gcgcttgctc aggctgtggc gaaacacctt atgtaaaatt attgacacaa atgttcggtg
     7261 atcgaatgat gatcgcgaat gcgacaggct gttcttctat ttggggcggt gcttctcctg
     7321 cttctcctta tacgaccaat gaatgtggtc aagggcctgc atggtcaaat tctttattgg
     7381 aagataatgc tgaatttggt tatggtatgc ttctagcggc acagactcgt cgcgaggctt
     7441 tggcaagttc aatcaaagaa gtattgccaa aagtatcaga agaactacga gaattgatgg
     7501 aagactggtt agtacacatg tttgagagtg aaggaacgca gcaaagagca gcaaaactgc
     7561 aagctgcgat cgaaagcgaa cgatcaattt tccctgaact ggaggatata tacaagagaa
     7621 aggatctatt cgtaaaaaac agccaatgga tgattggtgg agacggatgg gcttacgaca
     7681 ttggttttgg cgggatcgat catgtacttg ccagcggagc agacgtgaat atccttgttt
     7741 tggataacga agtctattcc aatactggcg gtcaaacctc aaaagcaaca ccagcttcgg
     7801 caatcgctaa attttcggct agtggaaagt atgcttcaaa gaaagatcta ggaatgatgg
     7861 cgatgacata tggaaatgtt tatgtggcgc aaattgcttc aggtgccaat caaatgcaaa
     7921 cgatcaaggc atttgaagaa gcagaaaagt atcctggtcc ttctctgatt attgcctata
     7981 cgccttgtat cacacatggc ttgcttggcg gaatgaaaga atcgcttaaa gaagcaaaag
     8041 aagctgtttc ttctggttat tggtcattat atcgatataa ccctacaatg gtcgaaaaaa
     8101 acaagaaccc aatgactttg gactacaaaa aacctgattt ctctgctatg cctgatttca
     8161 tgaggaaaca agttcgcttt tcctcgctag ataacgcaaa tccaaatctt gcaaaagctt
     8221 tatttgaaaa gactgtccac gacgctaaaa cacgttttta caactatgct gcactgacgg
     8281 gagaattaga aaaaatcaag aaacgacttg aaccagagga atctatcgat gtacccgtta
     8341 agactagaaa agaaaaaacg acggaccttg aagcagaaga aagaagagct gctagaagag
     8401 ctgccagagc agaacgacgt aaaaaaatgg aacaagctac aaaagaaaaa aaggaaaaat
     8461 aacagaaaaa atataatgaa taattctctg aagtttaaaa atatttattg cagagaaaaa
     8521 taaaacgcat acttgcaatt ttcttgaaaa ttatgcgttt ttctcaagaa aaatacgagc
     8581 atatcagcct ctaaaattaa aacatatgat atactagaga ggaaatctca aaagagaaga
     8641 gaggcttttt atgtcatttc aaatcgggga actttttaat ttggactatt ggcagcaaat
     8701 gttttccggt gacttgttct ctctgaattt tttggtgaac atacttgata ttctggttgt
     8761 ctggtatctt gtatacaaac tgatccaact gctgcgaggc acaaaagcga ttcagcttct
     8821 gaaaggtgta gctattttta ttgttatccg aatcgtagcg gaaatcattg gtctgcacac
     8881 cttgtcttgg ctgatgaacc aagtcattac gtacggggtg atagctgcta tcgtgatttt
     8941 tcagccggaa atccggagag gactggaaca tcttggacgc agttcgcttt tcaaacaatc
     9001 aaaaagcgaa cagcaggaag atgagaagat gatcttgtct tttgacaaag ccatccaata
     9061 tatgtccaag cgaaaaatcg gtgcgttgat cactatcgaa cgtcatactg gattagatga
     9121 gtatattgag acagggattg ctttagatgc agatatcact ggggagctgc tgatcaatat
     9181 ctttattcct aatacaccgc tgcatgacgg tgcagttatt gtaaaagaag gaaagatcgc
     9241 agtagcaagc gcctatcttc ctctatcaga aagtatgttg atccctaaag aattcgggac
     9301 tcgacaccgg gcagctgtcg gtataagcga agtcagtgac gcgattacca tcgttgtctc
     9361 tgaagaaacc ggggacgtca gcatcacgtt agataatgaa ctgatggctg gcttatccca
     9421 gcaagaatat ttggcaatct tgcgtagaga actaattgct gaagagaaaa caaaaaacaa
     9481 gaaaaacgtg attcaatctt tcctagacgg tatgactaag ggaggggatc gcaaatgatc
     9541 tcaaaagaga aacggacaag cattctttac agttttattg cattgctgtt cagtattgcg
     9601 ctgtacttca atgcaaatgg ccagtccgtt ccaaatacgc tttctggaaa tgaagcgtat
     9661 aatcaaacag ttacgggggt gccgatcaag atttcttatg attcaaagcg ctactatatc
     9721 catggatatg aaaatgcagt gactgtcaaa ctttccagtg caaaccgagt tcagttgaat
     9781 gcagaggcaa acgaagatac acggatgttc cgcgtcacag cagatatcac taagctgggc
     9841 gaaggaacac atgaagttcc gctaaaaatt caaaatttga gcagtgctgt cacagcaaag
     9901 atcgaaccat cgactattac agtaacggtt gagaaaaagg taataaaaga tttcgatgtg
     9961 gaaacgttga ttccttcaac gttcacacca tctggatatg aacttgatga tacttccgtt
    10021 agtcctaaaa aagtctctat cactacaggt gacaaaacat tggcagaaat caaacgagta
    10081 gtagctgatg tggacgcttc gatggtcaca gatgatggga ttaatagtga ggtcccaata
    10141 caggcgctga atgcagcagg ggaagtatta agtatcgtct ctgatccagt gcaagtaaca
    10201 gtgagagctg attcgatcaa gccaagcaag tcggtacgac tttatgggat acagcaagga
    10261 acaccagctg ccggggtaga aagctatgat ttcagttttt caaaactgga agcagaagta
    10321 agcggctcta gcgatttgct tgcatcgatc ggtgacagta ttgctgtacc gatcaatgtc
    10381 tcaggtatca atcatcgaac aactcgtaaa attgaaatac cagtagaaga ggggttaagt
    10441 atcaagcccg aatcagttag tgtggagatt acacccgtgt tgcaaacgtc aagcagtagc
    10501 tctgcttcga cgagcagtcg gacacaagaa aatacaagta cgagtagaag tatttcatct
    10561 gctgattcag gaacggaacc atcgacaaca accatttctt ccagttcgga atctgggaca
    10621 agcagctcga ccacaagtac agaagaaaca tcagaaacaa gcgaatcaac acaaatgagc
    10681 agaacaaatt cctcagaaaa ttaagttaaa ggagtaattt tacaactatg ggtaaatatt
    10741 ttggaacaga cggcgtacgc ggtgaagcca acaaggaact tacgccagaa cttgcattta
    10801 agctgggacg gtgcggaggt tatgtcctaa gccagcatga agaagaagga aaacgcccac
    10861 gcgtgttagt agggcgggat acaagaatct caggtcaaat gttagaagaa gcgctgattt
    10921 caggactttt atcggtgggt atcgaagtgt tccagctagg tgtgatctct acaccaggtg
    10981 ttgcttatct gacacgtttg caaaaagcaa gcgcaggggt catgatttct gcttctcata
    11041 atccagcaca agataatgga atcaaatttt tcggtgcaga tggttttaaa ctagtagatg
    11101 accaagagct agaaattgaa gcattattag atgctgaagt ggatgaattg cctcgtccat
    11161 cagctgaagg attgggaact gtagaagaat ttccagaagg cttattgaaa tattctcaat
    11221 tccttgtaca aacgatcaac ggtgaccttt ccggtttgac catttgtgtc gatgcagcaa
    11281 acggagcaac ttcaacaagt gttaatcgtt tgtttgccga tcttgaaaca gatttttata
    11341 caatgggcac aaatccagac gggttaaata tcaatgacgg cgtaggctct acccatccag
    11401 aacgtttagc tgagatggtc gttgaaaaag gagcagatgc tggactagct ttcgatggcg
    11461 atggcgatcg tattattgcg gtggatgaac ttggaaacat tgtcgacggt gataaaatca
    11521 tgtttatctg tgctaaatac ttggcagaaa aaaatcgatt gaaaaaagat acgatcgtta
    11581 cgactgtcat gagtaactta ggattccaca aagcggtgga aggaatcggt ttgaaagatg
    11641 tcgttacaca agttggtgat cgttacgttg tagaagaaat gcgcaaaaac gattataact
    11701 ttggcggcga gcagtctggg cacatgattt tcttagattt taacacaact ggagatggga
    11761 tgctttcagg aattcagtta ctgaatatca tgaaacagac aggtaagaaa ttatcagagc
    11821 ttgcagcaga ggtaacaatc tatccgcaaa aattagtgaa tatccgcgta agtgataaaa
    11881 acggtgcgat ggatgttccg gcaatcaaaa aagtgattga agaggcagaa gctgaaatgg
    11941 gatcagaagg aagaatccta gttcgtcctt ccggtacaga accattactt cgagtgatgg
    12001 cagaagcgcc tactgatgag aaagttaatt actatgtcga taaaatcgcg gcagtagtca
    12061 aagaagaaat cggactggat taagagatat tttcataaaa aata
//
LOCUS       DACDTO010000088        11693 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDTO010000088 DACDTO010000000
VERSION     DACDTO010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.088"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(617..982)
                     /locus_tag="IWK70_002346"
     CDS             complement(617..982)
                     /locus_tag="IWK70_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614850.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1058..1303)
                     /locus_tag="IWK70_002347"
     CDS             complement(1058..1303)
                     /locus_tag="IWK70_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9614851.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(1373..1627)
                     /locus_tag="IWK70_002348"
     CDS             complement(1373..1627)
                     /locus_tag="IWK70_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614852.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(1791..2114)
                     /locus_tag="IWK70_002349"
     CDS             complement(1791..2114)
                     /locus_tag="IWK70_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614853.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            2301..2374
                     /locus_tag="IWK70_002350"
     tRNA            2301..2374
                     /locus_tag="IWK70_002350"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2335..2337,aa:Ile,seq:cat)
     gene            complement(2759..3256)
                     /locus_tag="IWK70_002351"
     CDS             complement(2759..3256)
                     /locus_tag="IWK70_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9614854.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(3296..4105)
                     /locus_tag="IWK70_002352"
     CDS             complement(3296..4105)
                     /locus_tag="IWK70_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9614855.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            4439..5296
                     /locus_tag="IWK70_002353"
     CDS             4439..5296
                     /locus_tag="IWK70_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9614856.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            5289..6191
                     /locus_tag="IWK70_002354"
     CDS             5289..6191
                     /locus_tag="IWK70_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9614857.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            6224..6574
                     /locus_tag="IWK70_002355"
     CDS             6224..6574
                     /locus_tag="IWK70_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9614858.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            6608..7546
                     /locus_tag="IWK70_002356"
     CDS             6608..7546
                     /locus_tag="IWK70_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9614859.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            7624..8517
                     /locus_tag="IWK70_002357"
     CDS             7624..8517
                     /locus_tag="IWK70_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9614860.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            8555..9118
                     /gene="amaP"
                     /locus_tag="IWK70_002358"
     CDS             8555..9118
                     /gene="amaP"
                     /locus_tag="IWK70_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9614861.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            9132..9320
                     /locus_tag="IWK70_002359"
     CDS             9132..9320
                     /locus_tag="IWK70_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9614862.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            9333..10235
                     /locus_tag="IWK70_002360"
     CDS             9333..10235
                     /locus_tag="IWK70_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9614863.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            10267..10509
                     /locus_tag="IWK70_002361"
     CDS             10267..10509
                     /locus_tag="IWK70_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9614864.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(10765..11136)
                     /locus_tag="IWK70_002362"
     CDS             complement(10765..11136)
                     /locus_tag="IWK70_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614865.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4169 a         1986 c         2208 g         3330 t
ORIGIN      
        1 tattaaaaaa ggcataatag ttataagctt ttttaaagaa tatttagaaa ttattaattt
       61 tcgtctgaaa acaaaaaaag aaagcgttat tattttagtc tattttcagg gggggaacct
      121 tatgataaaa aaactatttt tactatcaat attaacgcta gcaatggcag gttgttctga
      181 ctctggctta gcaactgaac tgatcacaaa atcaactgaa gccacgacag aggagttaaa
      241 atgtggaaac gatcacagtg actacagagg cttaaatcga cgatgcatgg gaatattagg
      301 cagaagcaat tgtaagcatc gatgtagtaa tcgaagaagg tgtagaagaa gcaacagaaa
      361 aatcaaaaga aagatcaaaa gaagcaatct gtgctgtgca tcgtatacag acgtcgaaga
      421 tgcggtactt ttaaaccacg tcaggagatg ttcatttatg tctagggaag ttggcgatga
      481 accaaaacta gagtacgctt ttagttttgc acgtgaggat gaagataacc ctaaaactta
      541 cgacatttta gcttgatcta agatggttaa agctttttaa tgaagtaaga ggagatagaa
      601 ggcttttcta gttatattag gtaagataaa ttcctttttg ctccaaattt gctaaacatt
      661 ctgctagata aacttcatcg tgttccggat aaatcggcac gttgcgctct acagtaggta
      721 aatcaaaaaa tgcttcgcat gatttgccac gatacatagg atttttccac tctggtgctg
      781 ggctgtaacc acgtttttcc atttcatcca tgacaagctg atgatattgg aataatctat
      841 aaggagaata gtgaaaaaca taatccacag tagcatgttt ctttccccag ccatttcctc
      901 ttaatgcaca gcattcccga tgttgtccta aaagctgttg acgaggaagc tttgaaatca
      961 atttttcatg ccataatcgc atagaaatgt ctcctttata tatagattgt taaccttaga
     1021 cgttaaatga aaaaaccagc accaaggctg gaaaaaatca cttcagtttg cttgctgttt
     1081 ctgagcgacc aattaggtaa tcaacggaaa tatcgtaaaa gtcagcgata tagattaatt
     1141 caatgatgct tggaatttct tctccttctt cgtacttacg atatttgaat aaaggaatac
     1201 ttaacgcgct agaaacagct acttgagata aagatcggcc agttcttaag aaattcagac
     1261 gacaacgtaa agtatccagt aaaatagctt cttcgatact catttaatca cctttcatta
     1321 tagtagaata gttataatga ctatagaaag ctacacatgc aatcaatagt actcaatgtt
     1381 ttttatttaa acgtatcaaa taaagaatga cgatcaataa agacaaaacc agtcctgata
     1441 attcttctat aaaaacacta tcgggttcaa agtagttgta attaaaaact ttccacataa
     1501 agtagatagc cggaagagtc aaaatagcta aataaaaccg ttctttgttc caattcataa
     1561 gataaacaat acttgcgacc agtgcgaccg caggcaccca aaacaaaact ctcaccaacg
     1621 taatcatgta acggactcct caacgattca tataataaaa gataacatgt tattgcatac
     1681 atcacaaatg aaatgctagg aaaataaaag aagaatagat caatgaaaaa tgaaaaagca
     1741 gaacagtttt ctgctgataa gcaggaactg ttccgctgaa tgtgtcttat ctatcgagaa
     1801 aaaatcaatg gattgatatc taagtaattc aatacatata aaaagaggac gatcgcattc
     1861 actaaaaagg aaagaatgat gatcccatag cgaatggaaa catatttaac aaatactttt
     1921 gtttgaatac gggcatatct ttttccttta tagctgagca tcatgacaag aagcaaaagg
     1981 gtaatcacac cttgaaaaat cattccaata aacagaattc ctgaggtttg ataatttaat
     2041 tggagaaaat aagctgctgc cgcaactaaa tcgaataaaa tcgcaataaa tggcaaaata
     2101 atcactccta acattgagaa taaataaaag aataacattt tttctaaaat aagcaaataa
     2161 aaattaaagt aacaaaatag ttatgacaca agtaaaagaa ataagaataa caatattatt
     2221 ttacttatat tttgaaactt gtgagagttt gtcattcgtg ctatagttga aaagtaacaa
     2281 aaataaattt tatttatgat gtacctttag ctcagttggt tagagcagac ggctcataac
     2341 cgtccggtcg taggttcgag tcctacaagg tacataagag gttgtgaaaa agctgccctg
     2401 catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttact tggctgttct
     2461 tattactgca gatgcaagct tttgaatacc gtttaaggaa gaggttgtga aatcagcgtt
     2521 ttgacctgag tattagctta acaaacggaa aaatagttcc tcatattttg ccgtttgtta
     2581 agctaatacc gagggtcagc tgattgaaca ccatttaaga aagaggtcgt gagcctgccg
     2641 tttcactctg tgtcataaaa aagccaatct actttgatag aaaaagcacg taggactaaa
     2701 acagttctgc gtgctttttt tattgtgtgt taaatatcgt aaatgaagat agatattttt
     2761 atgattcgac gatagagacg ttgaaatctt tcaacagctc taaagcggat tctccgtgtt
     2821 ttctactgga gactaacatt ggatcaacaa aaaagtctgc ttcaggaaaa gcactctgca
     2881 ggcctatcag attagagagt acacacaagt ttgtttcaat cccgactact tcgatctctt
     2941 tttgttcttc tttgctttca aataaagttt cctggatttc agctaaagtt tcaggtggaa
     3001 tcgtaaagta atacttttta atcactcgtt cattttcttg tggagaaaga agagggatca
     3061 attgcaaatc ttctcgttct tcttctttgt ccttcagttc aactggtata tcccgtgtga
     3121 agagaatata ttcattattt tggcgtgctt tagctaatct ttgagcaata cgttctgcca
     3181 attgatctga ctctggaata taaaattcgc tgtttggatc aagaatatga ttttgcatat
     3241 caataataac taacattttc tttcctccta cattcatatc gtcattttat tctttttaat
     3301 agaagttttt taaaatataa gcaacaccag cttcttcatt cgtcagtgtt acttggtttg
     3361 caatttcttt tacttgtcgc tgtgcattcc ccatcgcaat actcaacccg ctgtaattca
     3421 acatagcaat gtcgttttct tgatcgccaa aagccatgat gtcactcgat tcgattccaa
     3481 ggtaatccgc cagaaaaaca agtgaatgag ctttagtgat acctgtaggc gtgatttcta
     3541 agaaatgcgg atcagagaat actgattgga taccatctgc ttgcagatct ctttgtagct
     3601 tctgtagttt ttcttcactc ttatctgtta atgctaattt aagatagcta agagaaggat
     3661 cttccaacat atttaaaatt tgatcaaaag aatggttctg taaattgaaa tgttcataat
     3721 aagggtcatc tttgactaga tttgattcat cgaatgtcag ctgatcatct tgggtttctc
     3781 ggctagcaat gatggatatt ttcgcttctt gagcatagaa gaaggctttt ctcagtgtat
     3841 cgctatctat ttttgaacgt ctgagaactt tcgccatttt tccatccttc atttcagcaa
     3901 tcagtgaacc attggaagaa atcaaatagc caccgattcc taattcggaa aaaatatctt
     3961 tagcagaaat gctgttcctt ccggtagcga cggttacgat tccgccattt tcatcaaatt
     4021 tccgaagtgc ttggatattt tcttctggta tctttccatt tcgatcaagc aaggtaccgt
     4081 ctaaatcaat cgctgctagt ttcattttat cttcctactt tctttttaca tatctatttt
     4141 cctgttacct taagattaaa tgagaattta aaaaaatgaa actaacttgc tttgtcatta
     4201 tgaatttttt caaaaaacat tcttaagaaa aaggtggcta tatatagtag tttttggtca
     4261 atacctttga ataaaaagcc atgaaaaacg aatagttagc gcttaccgat gcgttttgtg
     4321 ataaaaaaat tttaaaatat tcaatgaaaa caaagcatat attttgcttt aaaaccaaat
     4381 gaggaatatt ctagatgtag aacaggttag aaaaagatta gaaaaaggag tgattttgat
     4441 ggagaagaga tacggcgtag ttcctgtggt aactgcttca gacgaaaatt atgctcctta
     4501 cttgagcgtg atgatcgcaa ctgcattaga aaattgcaat aaaacaagac gcataaaatt
     4561 ttatgtgatt gacgatggtc tatcagaata tagtaaagaa ggattggaag aaacggtaaa
     4621 taaatattca agtaatgcaa gtatccaatt cctgacagtg gaaaaagata tctacgaaga
     4681 cttcctagtc agtgaccata tcacaactac cgcttactta agaatttctt taccaaactt
     4741 attagctaaa gaggattata agaaagtcct ttacttagat tcagatgtgt tagtactaga
     4801 cgatatcgta aaactatatg atgagccgtt aaatggaaaa acaatcggcg ctattatcga
     4861 tccaggccaa gtgaaagctt tggaaagatt aggaatcgat tccgatgact tgtacttcaa
     4921 ttcaggagtg atggtgattg acattgatca atggaataaa aaagaaatta cagaaaaaac
     4981 tatccattac ctcagtgaaa atggcgatcg aatcatctat cacgaccaag atgctttgaa
     5041 tgctgtactt tatgaagatt gggaacaact tcatccaaaa tggaacatgc agacttcgct
     5101 gatttttgaa cgtcatccag caccaaatga aaaatatgaa cgtcagtata aagaaggaaa
     5161 tgaaaaacct tcgatcgttc actttacagg acatgataaa ccttggaata cgttgaaaga
     5221 tcatccgtat accaatttat acttaaaaaa attagcacac agtactctaa cgaaagtagg
     5281 cgagatcaat gaataaaaaa gaaatagcag tagtcgcaag ttgtaacaca aaattcgtac
     5341 cacatttagc tgcattattt gtctcagtat tagataattg caacccgtct aaatttgttc
     5401 ggttctatgt gattgacgat gatatcgatt ttgaaagtaa acagctttta cgcttctcag
     5461 tgaaaaacgc acgaatgaac tcagatgtgg aattcttgaa aatcaataaa gaattcttta
     5521 caaatgtggt aatcagcgat cgtattccag aaacagctta ttatcgtatt gcgattccgg
     5581 aattgttccg cggcacagaa gttgaacgta tcttatatat ggattgcgac atgatcgctt
     5641 tacaagatat ctctaaatta tggcgtcttg acttcggtga ttcaatcgta gcagctgttg
     5701 aagacgctgg gttccatcaa cgactggaaa aaatgaaaat ttcagctaaa tcaatgcgtt
     5761 atttcaactc aggtttgatg ctgatcaatg tgaaaaaatg gttagatgaa aatattacgc
     5821 aaaaagtatt agatttcatc gaacataatc cagaaaaatt acgattccat gaccaagatg
     5881 cattaaatgc tatcttacat gaccgttggt taccacttca tccgcgttgg aatgcacaag
     5941 gctatatcat ggcaaaagca aaaaaacatc caacagcagc aggtgaacgt gaatacgaag
     6001 aaactcgcaa caatccttac attatccatt tttcaggaca tgtgaaaccg tggagcaagg
     6061 attttgaagg gccaacgaag aaatattacg aaaaatatgc agggatgacg gctttccgtt
     6121 gtgtagctaa attccctaaa tatccaaaat atgcaaaagt tcaaccaaaa acagatgtag
     6181 ttgttaaata atagaagaca acaactgaag agggggagta ggaatggaaa ttacaatgtc
     6241 taaaaatgca gtagagacgt taattgaaaa ggttggggaa aatacaaaaa tcgcactcgc
     6301 gcttatcaat gattccgatc catttctaag ggacaaaggt gcttttgcaa aaggcagctt
     6361 ttttcaaatc attccttttg tttcggaatt tggagaatat gcgactaaaa tcgagcatcc
     6421 attattggac atctatacat ctaaattaga gcaaaattat ttcggtaaac gattgaacat
     6481 ggacttcaac aaacagcttg acagcttttc attagaaaat gaaatcgcag tactcgatta
     6541 taatatcaaa ttaaagaact gcttcttcag ctaatagttt cgagaaagtc caaaaaggag
     6601 gtatacattg agatactact ttgtggatga agaaaagaat gtaatagaaa cagatgaaaa
     6661 aaaatataat tggctcgtac ttgatagtgc aaagcccgaa gagattgagc aagcaatcga
     6721 agaattcgag cttcctgatg atattttcgt aggaaccgaa tatccggaag aagtttctcg
     6781 gatggagcgc ctttatgaga cgaaactaga gcatccattt tctttggtag tgattaatct
     6841 agatcagtca gataatcgta tcgaaaaaaa attaacgcca atatcttttg tactttccga
     6901 taatctcttg attacttgta tcgatagaaa aaccgaattt gtggatcggc tgctgacaaa
     6961 gcatggaaat catttaaact cgtttgaaaa gatcatcacg tatacattat tagatattta
     7021 cacccattac gtgaaagaac taagagagat gaaaaagcga attgacgctt tggataaagc
     7081 agctcgtaaa acaacggaaa atgaagaact gtataaacag gcagatcttg aaagagacat
     7141 cgtatatatc gatcatacct taagagatca acgggaaaca atggatcaat tatggaattc
     7201 tgaaactttc atcaaacgtt tagacgatga acagctactc tatgatgtcc atttgagaca
     7261 gcgacaaaca gaaaaaatga tcgagatcta tcgagattta ttagagagta ttggtgatct
     7321 attcaacggg atgatggaca ataatctaaa tcatatcatg aaatatctag attcagctgc
     7381 attaatcatt tccgttccgg cgttgttcgc agggatttgg ggaatgaata caggaggatt
     7441 accgggtaaa agttctgctc taggctttat actcgtagtc tttggctctt tgatagcagc
     7501 agtagtttta ggttaccatt tataccggaa agattataca aattaaagaa tctatttaaa
     7561 acaggtatca aacaaagaaa tttgttttgg caatacaatt taaaaactag gaggaataca
     7621 gcaatgacag aaccaaaatt aatcgacccg cgaaacttgt accatacgga gaaatttcct
     7681 aaacaagatc aggatacacc agctttacag cagaatatga ttcctgttcc tgattgtgga
     7741 gaagaatcct atgaaggcaa taatcaacta gaaaaccgcc gtgtactaat cactggtgga
     7801 gattcaggga tcggtcgtgc agcagcgatt gcatttgcta gagaaggggc agatatcgct
     7861 cttcaattct tcccaggaga agaaaaagat gcagaggaag tagcaaaata cgtgaaagtt
     7921 gcaggacgta agattgtctt actacctttt gatcttcgtg acgaacaagc acctaaagaa
     7981 atcatagata aagccgtgag tgaacttggc gggctagata cgatggtact aaacgcagga
     8041 cagcaaattt cttgtcagtc tattgctgag ttaccacttg agcaagtcaa agacacattt
     8101 atgataaaca tcatcgctat gtttgcttta gtgaaagaag ctgttccgca tatcccagca
     8161 ggaggagcaa tcgtaacaac aacttctgtt caagcattca atccaagcga acatttatta
     8221 gattacgcag caacaaaagc ttcaatcgct aactttacga tcggtttagc aaaacagctt
     8281 gcacctaaag gaatccgtgt aaatggtgta gcacctggtc caatctggac accattacaa
     8341 ctagacgaag gacaaccttc tgattcgatc cctgaatttg gtcagcaaag tttactagaa
     8401 cgtgcaggtc agccaggaga attagcacca gtttatgttt tcttagcatc gaataaagca
     8461 agttatgtta cagcccaaat ttacggagta actggtggag aagcgatcaa cttataagaa
     8521 gttgatcgtt tcacccctat gaggaggtgc gaaaatgaat aaaggtgtaa aaacgatcgg
     8581 agtgatcgga acattacttt tgctatctgt gcttttgttt gcgatactat taagtgcaac
     8641 ctatccattg ccttttcagc tagagaagtt tcgattcttt tccatcacta attcatacgt
     8701 gcaacaatat gtattttggc ttgccgttgc atttgctatt ttgacaatta tcacgttact
     8761 tgttttcctg ttttatccaa aaacaatcgg aaactttcta ctgaaacatg gtgatgggga
     8821 gttgacgttg gataaaaaag caattgaagg aatggttcgt tctcacttgc atgaagatga
     8881 attcatccat tcaccaaaag taaatgtcaa agcaacgaaa aacaagatcg ccgtcaatgt
     8941 aaaaggggag ttgaaacgaa cttcagcact cattggtaaa acaggaacat tgatgaaaga
     9001 aatcgaaaat gaagtacaac acatattggg aacaaaagag ccagtgaaag tatcggtgaa
     9061 atacacaggc tttgaagaac cagctaaacg aagcaatgac cgcaaacgag tagaataggg
     9121 ggggaccaaa aatgaaagaa ctattttcga catacaagct ccctatcatc tttggggtac
     9181 ttggattgat attagccgtt ttatggatca ccgtgggctt tttcaagacc ttattgatct
     9241 tgatcatggc agcgatcggc gtcgctatcg gtttttatct gaaacaaacg aaaatcttag
     9301 atcactattt taaatcttag gaggaaacat ccatgaataa caataacaaa aataataaca
     9361 ataaaatgaa caaccaaaaa ccagtgtctg cacatccaga cccaacgaca ccaaaccaat
     9421 caaatggtgt gaacgatgaa aagaacaaaa cagcaccagt atctgcacat ccagatccag
     9481 tagtccctaa cgttcaacaa ggagtagaag agaagaaaca tgatgcagaa aagcatgcac
     9541 ctcatcatcc aggaactcca gaatctaaag ttccagaaca acatgatgca atcgatcctt
     9601 ctaaacctgt ggaagagcaa aaacatcctt ctgaccatgc gaaagcacaa gaaaaactta
     9661 gttctgacca cacaaacgga caaggaaaac ttggttctga tcatacaaat aaccaaggaa
     9721 agcatgattc tgaacaggaa gattcaatgc gcggtgagct gaattatgaa gataaagttg
     9781 tgcaaaaaat catcggaatc gcaattgaac aagtagacgg tttattatct gcaaacggtg
     9841 gtttcttctc taatgtggca ggaaaacttg tgaatacaga taatgtaacg gctgggatcg
     9901 aaacagaagt cggtaaaaaa caagtagcag ttgatatgga tgtcattgtg gaatatggca
     9961 aagatatcga aaaaatcttt gaagaaatgc aagaagtaat cggcaaagaa gtaaaaaata
    10021 tgacacattt agaagtgatc gaagtcaacg ctaacgtagt ggacatcaaa acaaaagaag
    10081 aatttgaaaa agatcaagaa acggtgcaag acaaagtaac tgacgcagcg aaaaaaaccg
    10141 gtgaatttgc ttcgaatcaa acagataaag taaaatcagc agctggaagc ggcgcacaaa
    10201 aagtaaaaga aaatacagaa ccacgggttc agtaataagg aagtaaaaca ttaggaggaa
    10261 cgaaacatgg gatttatttg gtcacttatc gtcggaggaa ttatcggagc aattgcagga
    10321 gcaatcacga ataaaggttc ttcgatgggg attattgcaa atgtcgtagc aggtttagtt
    10381 ggatcagcac ttggtcaagc attattaggt tcttggggac caagtattgc aggaatggca
    10441 atcgtgccat cattgattgg tgccatcatt ttagtactaa tcgtatcatt ctttgctcga
    10501 aaactgtaaa aagactttta aaaagaggtc gtgaaaaagc tgttcttcat caagtaataa
    10561 gaacagccaa acaaaaatag cttctcatat ttttcatttg gttgggcata ttatcgaaga
    10621 tgcaagcttt tgaacactgt ttatacggaa taagaggctg tgacaagact tttgtcacag
    10681 cctcttattt atgcgggcaa attcccaatt agatttttaa aagtttttcc ttaaatgtgt
    10741 ctttattgat acagagaagc ttctttaata aaacaatgcc tccctaaatt ttcttgagat
    10801 aggaaaggtg aacatacaaa ataatacgac cactttgaaa acccagatga tccacaaatg
    10861 gatcgtatga tactgatgaa gtaaaaagca atggatcaga aactcaaaga gattgaaaaa
    10921 gacaaggaag aaataatatc tcttttttac tcttgtctgt tttagccaat aatagctcgt
    10981 aaagtaatct agacaaacaa atagaagtaa acaaagtttc gtaagagcag caatcgtatc
    11041 atgttgctct gctagacgat tgacgagcag gaaaaatgtt ggtaatggaa tatagatcac
    11101 gagtaatgaa agaaaaaagt aaacaataaa tggcatacta ttcctcctaa ttgtgtgagt
    11161 tcgccctcat tttaaaataa cgatatttgt ttgtgaagct ttttcgtgtc gtaacaaatg
    11221 ttatctaatg tatttttaaa taaataatca ttattatcaa aataatgtta aaaataaaaa
    11281 agaattgtcc atcactgtta ttcaaatcat tttatcaaaa ctacatagta aatgttaaaa
    11341 agcaacatga cataatatat agaaggcttt acagtatata tgggagctta ctaaaaaaga
    11401 atggagtaag agttaacaca atgggccatt agctgaagtt ttaagcactt caaggtatga
    11461 aacagaatgg cctgtaatac tacatgatta tttagactgc tcaaactgct atcagtctgg
    11521 tggaatgaat aaagtattat actaaaagca gatccaacta caacgtaaca ggaaaccagt
    11581 tgtatcagga aaagtgatag tacatggaga agaaaaggcc ccctttgtat gagtcagtac
    11641 atatcttatt tatagataaa acggtattca atcgaaataa aaaatctgaa taa
//
LOCUS       DACDTO010000089        11625 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDTO010000089 DACDTO010000000
VERSION     DACDTO010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.089"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(119..1249)
                     /locus_tag="IWK70_002363"
     CDS             complement(119..1249)
                     /locus_tag="IWK70_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9614866.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1790..1996)
                     /locus_tag="IWK70_002364"
     CDS             complement(1790..1996)
                     /locus_tag="IWK70_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614867.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2012..2626)
                     /locus_tag="IWK70_002365"
     CDS             complement(2012..2626)
                     /locus_tag="IWK70_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9614868.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2773..3456)
                     /locus_tag="IWK70_002366"
     CDS             complement(2773..3456)
                     /locus_tag="IWK70_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9614869.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3565..4023)
                     /locus_tag="IWK70_002367"
     CDS             complement(3565..4023)
                     /locus_tag="IWK70_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9614870.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4107..5870)
                     /locus_tag="IWK70_002368"
     CDS             complement(4107..5870)
                     /locus_tag="IWK70_002368"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9614871.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6091..7551)
                     /locus_tag="IWK70_002369"
     CDS             complement(6091..7551)
                     /locus_tag="IWK70_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614872.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="IWK70_002370"
     CDS             complement(7759..8685)
                     /gene="glsA"
                     /locus_tag="IWK70_002370"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9614873.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8708..10135)
                     /locus_tag="IWK70_002371"
     CDS             complement(8708..10135)
                     /locus_tag="IWK70_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9614874.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10166..11380)
                     /locus_tag="IWK70_002372"
     CDS             complement(10166..11380)
                     /locus_tag="IWK70_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9614875.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3522 a         2404 c         1916 g         3783 t
ORIGIN      
        1 taatatgatt ttttgagata ctttcattgc gaattcctct ttctaaaaga acctctcgct
       61 tgaaaaataa aagattgtac tttatgataa ttcaaactga gagtttctca gtttctcttt
      121 attgtttttc aggtcgatag agcgccaact cttcggcctg tatctttgaa gaaatattca
      181 acgtgattcc gattcctaaa gaaaggatca gataacttgt tcctccgtag ctaacaaatg
      241 gcaatggcac accagtcatt ggcatcagcc ccgcgatact tcccacgttc attatcgtct
      301 gaacgaatag caaagttccg aatcctaaaa gaaacaatcc agcttgctga tttttacagc
      361 gactgcttaa acaaaagatt ctcatgcata atgagaacaa taagaaaagc acacacaatg
      421 caccgattag cccaagctct tctgtaataa tcgaaaaaat aaaatctgtt tctgtctctg
      481 gaagataccc tttttttgtg atactatttc ctaatccccg cccaaatatg ccaccattat
      541 atagtgcata aaacgaatgg gtcatttgat agcccgcacc atgactatcg ataaaagaat
      601 ctcttaaagt cgcgatcctt tcataagcat gattaaacat ctgtggaagc caacctttat
      661 ttcctaaata taataccccg cctgctgctc caatcagcaa tgcagaaaaa gtaacgatta
      721 gatagatccc ttttttaaat ggaaccgccg ctgcccaaaa tatgacccac gcaatcgaaa
      781 ggatcatcaa tgctccagca atctttggct gaaataaaat cagaaaagtt atacctactg
      841 taatcaggaa tggttttttt agatcttttg ggctactatt tccgtcacga aaaaaccagc
      901 ttaaataaaa aatcaaaaaa agatttgcca gttcagaagg ctggaattga atgccaaaca
      961 gagagatcca acgttgtgcg ccgttgacag tgacaccgaa tatccctatt cttactaata
     1021 ctaaaaagaa aatcgataag gctaaaccgt atcctgctat tttagggtga agtaaatagt
     1081 gtagtttgac tgaacgagct aagaagatca cgctccaact caaaaatatg aaaattagtt
     1141 gacgcaataa tagacttttt gtattttcat cggcttgcaa taaacgataa gaactggcac
     1201 tgtatacttc taaaagaccg atcatggaca aagtcaaata aggacccaaa atccaccaat
     1261 cgatcttttt tcaccgcatc tcctcctcac acagcaatcc acttgtcagg tgaggagcga
     1321 gtttgtctca tcaaataagt cagtcacaaa aatataaaga aaaggtcaga aaccttttcg
     1381 aacaagaatt ctgacctagc atcatctcat cgtccgagtt ttagcactat acaacgtaaa
     1441 gaaaacattc ttagctattg gaaaaagcct taatccaaca gtttctgttt tacccattgg
     1501 cgactttcga cgtttctggg caacagccta tgtttgtata ggagcctcac ctaacaaggt
     1561 tattactata ttctatttac gagttcatga tagacttttc ctaatgatta ggcaatggaa
     1621 tttgaaatga aatagtgaaa tttaacaatc tttaatattt tttagaaaaa aataacaaat
     1681 aagttgaaag aatcccgttg caaattcttt cagttgcacc aggattcttt gataattttt
     1741 tagttttatg tgttttttta tatgtttttt cacttttcgg aataaatatt tatttgttaa
     1801 agtaattaaa caatccgcta atcgtttgat ctggcatttc tatttcctta ccatctaaag
     1861 taacagtagt aacgacaaaa tggtcttgca atggcggaac ttctaagtac tgcccttgca
     1921 gaaaatcatt cactgtttca tattcttttt tcatttcttt tgaatctgtc gatggtttat
     1981 agcttatttt taacataact agtgctcctt tttattcggg acgtgcaatc gtgaaaattt
     2041 ctcctattgc tgcataatca tttcctgcca aacggcttac agcattcaat tttcttggat
     2101 cgatccttcc tgtcttatca tcatagactg tttcatttaa atgatatttc tgtacttcta
     2161 gcaaaaataa atcagctgtt actttatttt tattccgtat ctcgacatgt tcatataagg
     2221 atgtttcaaa acgaactttt gcttctgtca atgctggtac tgaaatgatt actgaatcag
     2281 ttaaagtcaa gtcagtcagg tacagttcgc tttcctcatt aggcagattg gctgctgttt
     2341 tatttacttc ttctacgttg tcctcatcaa ggatatggat aacagcttct cttgtctcaa
     2401 gaatattctt tgctgtgtct tttactgcat cttgttttct ctggatcgcc aaggataaga
     2461 tgggtcgatt cgaactgacg atattgaagt aactgaaggg tgcgatattc actatatctt
     2521 ttgctgattt agtcgttact actgctactg gtctagggat gactgaacca atcagcaact
     2581 tgtaattttc acgttctgtc aattctgaag gactccgttc aaacatggtt tcgccttctt
     2641 tctataaaat tcgttaaaaa gcagatcttg aagacaaaat gaaaagtaat gcttttttca
     2701 tcttcttaga atctgcttta aactatctat caatttttct tttttgtcaa gaatctgtgc
     2761 cgtgcgtgtt ttttagttat ttgctttact tgcggcttcg acatatcttt tagtgatcaa
     2821 ttgcggacgg gtgataatag atccaactac gactgaaaaa gcacctaatt catatgcttt
     2881 ctctacttgt tcaggagagt cagtatgtcc ttccataatt actgggatat cgacttgttc
     2941 tatgatttga ttcaataaat caaaatcagg tgtctgcgtc tttcgtgaat attctgtata
     3001 gcctgctaaa gtcgtagaca aaagatcaaa accatcttct tgcgcttgaa gcgcttcctc
     3061 aaaagtcgaa atatctgcca tagctaaccg accgtgttcg tgaatcattt ggacaagttc
     3121 agcagttgtt ttttctgtcg gacgtttacg agaagttgca tccagtgcaa tgatttctgc
     3181 ttgcgtctcc aaaagttctt ccacttcttt ttctgtcgca gtaataaaca cttctgaccc
     3241 atcgtaatct cttttgataa tccctattac cggtaaatca acgattcgct tgatttcatt
     3301 gatatcttct gtcgtatttg ctcgaatacc aactgctccg ccttcttttg cagccaaagc
     3361 cattctcccc ataataaaag aactgtgtag cggctcgttt tccaaagctt gacaagaaac
     3421 gacgactccg cctttgattt gttccagtgt atccacaata tctcctccag taatgttata
     3481 taaaaataca gcttcatata aaaatgtaac ccttttctat tcaggagaca ataatgagct
     3541 atttctttca gcagaccttt cttactaaaa gaaaattact tcaaattctt cgtatacgac
     3601 cagcttctct tctgtaaatt ctatgttcaa cgcttcttta taagcatgtt taaaaaactc
     3661 agcatggact ttccgccaat aagctaatgt ccgatcccct tctccttctt tccaagcatg
     3721 ctctttcgtg acttcactaa aaggcaagat ggtcacattt atatttttga tgatacaaac
     3781 tggttcattt tttccattca ggaccacgtc taattgacca acttttggta aaggctcatt
     3841 ctcttgttta tacagatcgt aaccacttga tgtaccagtc tttatctttt ttacaactag
     3901 ttcgctcaac tcgtctgcca ttttttctgt atccccaaat gaaaagaaac ctaatttttc
     3961 tgattgataa ctaggatatt tttcttggaa ctttttgaca tattcttcta cttgctgata
     4021 cataacatcc acccctttgc tttttataat gaaaaaacca tccagatact agtataaaca
     4081 attatctgga tggtgagcat aatgaattat tgaatattca agacacgcag actatcttca
     4141 acgatttgtt ttggtgttaa atgataacgt tcataaagct gccctacagg aacgctatcc
     4201 gtgaattctt tctctgcacc aaaatttaat acgttcattg aagaatcacc ataataacga
     4261 ctgatatttt cgccaaatcc tcctgacaaa ctaccatctt ctaatgtgat gactaattcg
     4321 tgtgtttctt ttaatttatc taatgtttct acgtcatttt ctgtgatcgt tcctggattg
     4381 atcaacgttg cttcgattcc tttttttcta aaagctttta ctgtttcttt tcctaattca
     4441 agaaaaccgc ctaaagctaa aattgctact tttcctccat tatttactgt ttgataggct
     4501 ggaatactat aatcatttgc gatagctgta ccatgttcta caccatgtgt aggaattcgg
     4561 attgctacag gctgcgctgt ttgttcgaac gcccaatcta gcatagagat catatcctct
     4621 tttgttgctg gagcaagata tttgatgttc gggatacttg ataagtaagg aatgtcaaat
     4681 gttccttggt gtgttcggtc actagctgag atcgttccgc caccgactaa aataatcact
     4741 ggattctcgt tgatcgctaa atcatgtgat aattgatcat atgcccgttg caagaaagta
     4801 cttgattgga agacgactgg acgtactccc tgagaagcca aagcagaagc gaaagtgata
     4861 gagtgctgtt cagcgatccc cacgtcaaaa taatgatctg gatgtttttc ttggaatgtt
     4921 ttcagtccaa aggcgcctgg tatcgctgca ttgactgcgg caataggagc tgtacttgtt
     4981 tgcattttct tatccaaata atccatgatg atcatattaa atgtttctcc agaagttact
     5041 tgctttgcct ccccggtgga cagatcaaat ggtacatgcc aatgatattt cattttatta
     5101 tcgattgccg gctgataacc gcgacctttt tccgtatgga cgtgaagtac aatcggatga
     5161 tcaatatctt ttacttcttc aaataaacgg atcaccgttt ccagatcgtt tccattatcc
     5221 acgtaacgat aatcaaatcc cattgcttta aataggttat tgctgctttt accgttggtt
     5281 tgacgaagct cagctaagtt tttatataaa ctgccatgat tctcatcaat agacatctca
     5341 ttatcgttta ctaatacgat tagattggat tgtaaaacac caccattgtt caatccttca
     5401 aaagctaagc caccacttag ggagccgtca ccgataaatg ctaccacatt tcctgattct
     5461 ttcttcagat ctctcgcttt agctaaacct gttgctaagc tgatagatgt agatgtgtgc
     5521 cccactgtaa aatagtcatg tttactttca tgttggttgg aatatccgct cacttcacgg
     5581 taatgtcctt tttcaaaacc attttttcga ccagttaaaa gcttatgacc ataagactga
     5641 tgggaaacat cccaaacgat tttatccttt ggtgaatcaa atacataatg aaacgcaatc
     5701 gttaattcta ctacacctag attcggacca acgtgcccac ctacatcgct gtccttttgc
     5761 aagatttttt ggcgcatctc ttctgccaac tgtttcattt cctcaacaga taattttttg
     5821 acatctgcgg ggctattgat tgcatccaat acatgtaata ctgtactcat aatacattct
     5881 ctcctccgta ctatcttctt ttaatgaaaa tagatttgtt acactttatc tattctacta
     5941 cttagagttg actctaagtc aatagcattt taaaaaaatt tcagagaaag cttgttatat
     6001 aagtagatct tcgcacgaat caggactcaa ataaaaacct tctacagaca attaaaaagc
     6061 tttgtagaag gtctcctaat taaggtctca tcaagaaata tgttttttta ataggtgaag
     6121 ttcctgagca atatgataca aatggatgtt tgcacctttt aatgagattg gatagatctt
     6181 tgtcttttca gtttctaatc cattttgata gctagcagca tcgcacaaaa ccactctttt
     6241 tttcgcacat agacattcat caggcaattc ttctaatgaa cgaaataaaa taggatttat
     6301 ttcagcgatg ccttcttccg catacaaatg aaattgggtt cgataaaaaa attgtttgac
     6361 tgaactttct gaaacaacaa aaatttctat ttcctgtctc cccgcttgtt cactgttgat
     6421 caaagaaaga cttttgacga attttcgaat gaaattttca ttccatctta gttggatttg
     6481 atttttcttt ttccactctt ccaatatttc tacaattttt ccgtacagaa gttgttcttt
     6541 ttctgataga agatagatcc tttccggtaa aaatacttgt ccatctgccc acaacgtacg
     6601 gacaaattgt aaaaaggatt gcttgaaaat aaaattttct gcatcgtcga tttcagcgat
     6661 actaataatc tgtaaaatca attcattcag tgataggtca ttgagaacaa aatttttata
     6721 aacaacttcg aaatcttttc ttaacagctc catgttctca ttggtataat ttctactgtt
     6781 gaacaaagaa aaaatataat agacatcttc ttcttgtaag tctagaattc ctttttgctc
     6841 ttccaatcct tgacagacta attgaaataa cggcttcgtt tctgcgcttt tcttatcttc
     6901 ttctttcagt ccaaggggaa atgcctttct tccgattgcg atctctattc ctcgtatgat
     6961 aaaggacttt tcatcttccg aatatcttct tttaaaaaaa tgagtctcta cataatcaat
     7021 caactcgtta caagcttctt ctacatacgg atttttattt tcatagcctt cccaattgac
     7081 ataacgtact aaagctagca agatgttccg cgagtcaaat tctgggataa tgatttcttt
     7141 atttttcaaa taatcatttt ctttaaaaaa atccaatacg atttttttga ttgtatatac
     7201 tttgctcaaa gaaataaatt caagatcagc cagttctgta ctcgtgaata taccattaaa
     7261 ataataactt aaaaacttta aaaaataaga ttgaccgtac agaaactgtg tgagctcaga
     7321 aagagcaata ccatgtttga atacgagttt atacttatgc ttctctctta cttgttcttt
     7381 tatacaatat tctagacact cggctagact ttctagatcg tgaaggatcg ttggtgtagt
     7441 cacccttaga gaaatagctg tttgttcgat gtctatttct tggaattcaa ataattgttc
     7501 acacagatac acttttctaa atatatttcg ctcgatgtaa ttttccagca ttcctgatac
     7561 acgcctccag tttttcttat gattttattt aatcatatct gtaaatcgta tttttgtttc
     7621 ctttttattt agaaaagccc tttcaaaaac caataaaaaa agaaactggg acaaaagtca
     7681 aacaagctca caatttcagc ctcattgtag gtagctttga gccgtttgga cttttacccc
     7741 agcctctttc aagaatggtt attcaaagat gtcagcgtct agttctttga ctacatcttt
     7801 caacaattgg ataccagctg cgctgttacc aaatggatct agtgccgggc tgaagacacc
     7861 tatgccgtaa cgatttggca cagctgccat caacccgccg cctacaccgc tttttgcagg
     7921 tacaccaaca tggacggaaa actctcctga ttcatcatac aaaccagcag ttgtcataat
     7981 tgatttgacg attgtagcac tttcttcagt aatgatttgt ttgccattcc aaggagcaat
     8041 tcctttattt gccagcacag cagctaattt tgctatgcct gtagcggtga cattgaggga
     8101 gcattgtctg aaataagcat cgagtatctc cggtacgtct ccttcgatca tttgattacc
     8161 tttcatatag taagcaagag agcgattgat atcacctgtt cttgattcag aatgatagat
     8221 ttcttcattc aatgtgatat taggatcatc acagatctct ttcataaatg acaagattcg
     8281 ctcaaatttt tcatctgcat ctttaccggc aatcaacgag gttgttgcaa tcgctcctgc
     8341 attaacaaaa ggattcattg gatgtttgcg atgattgatt tccatattca tgatagaatt
     8401 gaaggcaaaa ccggttggtt ccgtgttgat tgtttgaaag actttttcta ttccgttgtc
     8461 tttgattgct aataacaata cgggaacttt tgacatgctt tcgatggcaa agcgcacatc
     8521 ggcgtctcct ccctcaatca gttgttttgt atccaaatca taaatcgcca tacctaattg
     8581 tttgggatcc acttcagcta atgctggaat gtaatccgcc acttgtcctg tttgataata
     8641 gtggaaattt tttccaatca attgttctaa ttcttttact tccatttgaa taaccagctt
     8701 tcatttttta tttttctgca tgctggaatt tatgacggca tgcgtagatc gtaaatggaa
     8761 taactaagac gataagataa gaaacaatca gaatcgtttc ataaatccca tgctgtccag
     8821 ctgcgatact agcaggtgga acaaatgaga tacaaaaagc aaaaagtgaa gtcaaaagac
     8881 ctaaacaagc aacgattact tttacaactt ttccgcctgg aacctgatag gctctttcaa
     8941 gactgtcatc tttaaaaacc agcttcaaat agcccgcgaa aaacataaag tacaacatca
     9001 ggtagattac aacagtcaat gacatcgctg caagaaatga aagattattc cccccgccgc
     9061 ttaatgtaaa gacggctgcc cagattgttg caacgatccc atttgcgatc atcaaatgag
     9121 caggaacacc gtgtgtgttt tcttttgcaa aatattcagg aagtaatcct tcttctgctg
     9181 ctacgtgaag tgcttttgaa ggagaggtta cccaagaagc aatttctccg atgacaccta
     9241 aacaaacaag aatcgcaaga attttcgcaa tccatgcaag atttggatca atgtgtctta
     9301 ataggaattc taacgattga atcacccctg tattaagaga caaaccatgt tgaggtactg
     9361 tagtagcgac ggtcaatccg ccaaaagtat ctaaaatgat tgccagaata accaatacaa
     9421 acattgctaa tggataatcc tttttagggt tttccatttc atttgcatga ctggcagatg
     9481 tttctacgcc catatatgct aaaataaagg aaacaaaaac aaccagcgta ggcaatttcg
     9541 aaaaatcagg aatcaatgtt ttcatagaca gtatcgtttg tagtggagcg cctgaagcga
     9601 cataatggat ccctaagcca aacaagacta atgctggcaa caagatacca gctacaaaac
     9661 cgatccgagc aatcaatgaa gtgtatttgg ttccttttaa ctgtgagagt aaaatagccc
     9721 agaaaataat caataggatt gccaatttca gatatttatt gttttgtata gcaccaatac
     9781 ccgttgcata ggataatgca ccagttaaga aatacagcat ggtcaagtag ccaactgtta
     9841 tctcaaacca ttggaaaaag attgcggcaa aaccgaatcg ttttcccaat gtgcgactga
     9901 cccaagtata gacaccgcct ttttcccatc ctttcaccgt tgccatctct gctgcgcaca
     9961 atgccacagg gataaaccat agcaaaccgc ctaataacaa gaaaaatact aagctgaagc
    10021 ctgaggtcgc gaatgtggga tattcatata atgaaacgac cattgagatg gtcatagcca
    10081 aaaagccaaa gaacgttaat ttttttgcag gaattttcgt tttgctttgt tgcatgaatc
    10141 ttcctctttt ctatttgaat ttgtttcaat tgagtttgtt gccaaagaag aaaaagtcca
    10201 atccttcttc ttgttcttct tttgtagtag tgatcgttgt aaagtgcatc gccgatttta
    10261 aaatcactgt tgtcagcaca taggcataca cggcagtaat gatgacacca atcacttgaa
    10321 tccctaagaa gtaccagctg cttggattcg ttagtaaatg gctgttagat gcaaagatcc
    10381 ctatcagaat cgtacctgtg aatccgccca tgccatgaac accccacaca tcaagtgcat
    10441 catcccattg tttcttttta cgaaaatcaa cagcagcatg acagacgata cccgcaatga
    10501 tcccaatcag catggcactt tttgcttcta cataaccggc acaaggcgta atggctgcca
    10561 atccagcaac ggatcctgtt agtacgtcga caaaatcaaa gccatttcct tttacttttg
    10621 caatgatcag ccaagtaatc atcgcaaatg caagagcgat gaatgttgaa ccaaatgcat
    10681 tgactgcttg ttcatctgct cgaagtgcac caccggaatt aaaaccgaac cagccaaacc
    10741 aaagcagtcc tgtaccgata gcagctgcca taagactgct atgctgactt ctttctccta
    10801 aaggaatctt tctttgacca agtgtgagga tacatgctaa agagccgaat ccagctgtcg
    10861 tgtggatgac tgctccacca gcgaaatctc taaatcccat ctgatccaaa aatccgccac
    10921 cccatatcca gtggcaaaca gggaaataaa tcagcagatt ccaaaaaata acaagtataa
    10981 tatagccttt taaattcaat ctccctgcaa aagctcctgt catcaaagga acggtaatca
    11041 cgataaacat cagttggtaa agaaagaaca ttaaaaatgg aatcgttgca ccatgaaaca
    11101 tgttggggaa gcttgtaaca tgtctcatca aaaagaaatc tgccgggttt ccaataacac
    11161 cgccgatatc ccgtccaaag gccaatccga atccaccaaa tacccacata atcgttgtta
    11221 tcccaacgga gagaaaagat tgagacatga tcgttagtat attattcttc cgtgctaatc
    11281 cgccgtaaaa aaaagctaga ccaggagtca tcaggcaaac cattgctgta cataaaatca
    11341 tgaatgctgt atctcctgta ttgataggta atgtattcat ttttttcgcc tcctttctac
    11401 gattacgagt ataaaagaag gtgtagttgt ttttttcttc tgtttttttg aattaatact
    11461 tgcatttata aaagacagac aattaataaa agcttgatgt gttgtgctct ttttcgtttt
    11521 tcacacaaat aaagacaaaa aataaacggt cttcttttta tagaaaaccg tttattttcg
    11581 tcttttttta tttttaaaga tattttttca taaggaataa agaat
//
LOCUS       DACDTO010000090        11590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDTO010000090 DACDTO010000000
VERSION     DACDTO010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.090"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            398..892
                     /locus_tag="IWK70_002373"
     CDS             398..892
                     /locus_tag="IWK70_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614876.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(1054..2226)
                     /locus_tag="IWK70_002374"
     CDS             complement(1054..2226)
                     /locus_tag="IWK70_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9614877.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2432..3247)
                     /locus_tag="IWK70_002375"
     CDS             complement(2432..3247)
                     /locus_tag="IWK70_002375"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9614878.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3397..3843)
                     /locus_tag="IWK70_002376"
     CDS             complement(3397..3843)
                     /locus_tag="IWK70_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9614879.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3840..4844)
                     /locus_tag="IWK70_002377"
     CDS             complement(3840..4844)
                     /locus_tag="IWK70_002377"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9614880.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(4884..5213)
                     /locus_tag="IWK70_002378"
     CDS             complement(4884..5213)
                     /locus_tag="IWK70_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9614881.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(5339..7666)
                     /locus_tag="IWK70_002379"
     CDS             complement(5339..7666)
                     /locus_tag="IWK70_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9614882.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(8509..8802)
                     /locus_tag="IWK70_002380"
     CDS             complement(8509..8802)
                     /locus_tag="IWK70_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9614883.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(9205..10383)
                     /locus_tag="IWK70_002381"
     CDS             complement(9205..10383)
                     /locus_tag="IWK70_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9614884.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10487..10801)
                     /locus_tag="IWK70_002382"
     CDS             complement(10487..10801)
                     /locus_tag="IWK70_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9614885.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            complement(11121..11516)
                     /locus_tag="IWK70_002383"
                     /pseudo
     CDS             complement(11121..11516)
                     /locus_tag="IWK70_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         3357 a         2266 c         2020 g         3947 t
ORIGIN      
        1 acgtacatat tttattattg catacttaat aacagaacta tagcaaaaaa tcccgtgtat
       61 caacacggga ttagattaag tttcctcaat cactattcat aacacaatta ttataacaag
      121 taagtaaaca attgaaaagg acatttattg aaaacggttg ttttttactt aaggaggaaa
      181 tttagtgaaa aaattagttg cgttgttttt ggtcgcagga agtttgttga gtgggtgtgg
      241 tgctactacg tctactactc aaagcacaag ttcagctcag gcttccagca gcagtaccac
      301 gatctccgaa agtacggcag attcatcgaa agaacaaatt gaaaagaaaa aagaagaaga
      361 gaaacaaaaa gcggaaaaag ctgcactaga aaaaaaagtg acagaagcgg aatctgcttt
      421 gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag cagctatcca
      481 agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg attccacaat
      541 caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac gacaacaagc
      601 agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga ggcaagaagc
      661 aatcgcacag caacaagcag aacaggcgcg acagcaagca gcagctgaac aggcttctca
      721 gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg tcacaccgac
      781 cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg ccactttaag
      841 cgaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact aattcaaaag
      901 ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagctgg cgttccagat
      961 agtaaaccaa aaatagcagt ttacacgtcc atttcgtcag cttctctctt ttttcattta
     1021 gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca aatagtacac
     1081 ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa actgtttcga
     1141 atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc gatagttcct
     1201 gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca caaatggaac
     1261 tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga ccattccttc
     1321 gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc gcttagcgat
     1381 ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc caagtagtat
     1441 accgataaac caaataaaaa atttccattt tttccattga tcaattcctt ggatcgcttt
     1501 ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc gatgtgtatt
     1561 gaccatataa ggagtgcagg taagcaaagt cacgagatct ctgccttttt ggatacccaa
     1621 agaatctact tcgtcaggta agaccacttg gattttttct acttgatacg ccaatgtctt
     1681 tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat ctgtaaataa
     1741 ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt tcccgccagt
     1801 cggataagaa cttccttcca ataatgacgt tcctttttgc aaaaatattt ccgttgtctg
     1861 atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa gcatatgttc
     1921 cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg cttggttgaa
     1981 actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct ttgtcatttt
     2041 ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt gataatggat
     2101 gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat aagaaaagat
     2161 cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta ttctgctttt
     2221 attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa tcagcgcctt
     2281 gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc gtttgttgag
     2341 cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat cactatcgtt
     2401 tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga tggcgttttt
     2461 tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat acgatgatcc
     2521 ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt gtttcatcag
     2581 aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc ttaggtaatg
     2641 cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc agcccttcga
     2701 ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca gaacctgtga
     2761 cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt tttttcatta
     2821 atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct aatttttctt
     2881 gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca ttttctacag
     2941 taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga ttcccaagat
     3001 tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg ttttttacta
     3061 taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct tttgtttcaa
     3121 ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca gtatcagttg
     3181 tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt tcatcaaaac
     3241 cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct aacttatcta
     3301 cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg acatttttag
     3361 catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta acacgatatt
     3421 tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag catctttccc
     3481 atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg ttacacttgc
     3541 gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg tagcagtgat
     3601 gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg tcaattccgt
     3661 tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca aattaaccgt
     3721 aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta ggccgagaac
     3781 gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt tgatgttttt
     3841 cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca agctgtccct
     3901 gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc ttcgcttgtt
     3961 tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat ctgaactggc
     4021 gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa tggtatagga
     4081 tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg actcaatata
     4141 tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa tccatggaca
     4201 gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc tttggctacc
     4261 caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac tagaaattga
     4321 gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt gcctcctgtt
     4381 tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac atcgttatca
     4441 aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt taattttttt
     4501 ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg ttttattttt
     4561 tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt ttctggttct
     4621 agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc gttgatgttc
     4681 tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa atctgtctgc
     4741 ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc gctctttgga
     4801 tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag cacaacgaca
     4861 aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa tcggtaaaca
     4921 gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc ctcttcctcg
     4981 tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt ttgtgctagt
     5041 tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa ttcatccgag
     5101 acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa gttatccggg
     5161 atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa cattcgatta
     5221 tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc tataaaaaag
     5281 accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt tgtttttttc
     5341 atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca gaaaaaataa
     5401 agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa ggaagcaaga
     5461 gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa ttattgttta
     5521 cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc cggctgttaa
     5581 ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg agatttccaa
     5641 ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca gtaatcactg
     5701 cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta aatgacaact
     5761 tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt gtatagctgc
     5821 cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata ctgctaagtg
     5881 ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca tggattgacg
     5941 ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca ccgctgattt
     6001 gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt gttctggcag
     6061 ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca atttcagtag
     6121 gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga ttccatgtga
     6181 tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta tcattaaagg
     6241 catatttaac gatcggtaca acagaaagac catcatagta cttactgcca tttgcattga
     6301 ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt tttccatttg
     6361 cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc attgctgatt
     6421 ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata gtaggcgtat
     6481 tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta gaaggagcaa
     6541 tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg cctgtatagg
     6601 tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt gcaaaagaat
     6661 agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt ccagataatg
     6721 tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa aaagttcctg
     6781 tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt gaaacatttc
     6841 ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga atatcatttg
     6901 ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc cctccccatg
     6961 ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg atcgtatttc
     7021 cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta ccatttactg
     7081 tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg attgctttgg
     7141 ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca acaatgactc
     7201 catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg aaattcgcag
     7261 tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct ttctcccctg
     7321 ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc gaggatgtgg
     7381 ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt atttcagaat
     7441 agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt tctgtataaa
     7501 cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg atcgaaatca
     7561 taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca aaatgttttt
     7621 gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct cctctcaata
     7681 aaaaatcatc aaataatcct ttacacgtta ttctaaattt aatttttatt attttattga
     7741 tgtatagaaa gtttggggct ttttttctct ttttattcta aggttatttt agtcttgtcc
     7801 tttaatacaa agattttatt tttaaataaa aaatactctt tttttacgat tattgaaaaa
     7861 aaaagataaa acttgattaa ttgaagtatt gcttaaattt ggaagctatg agggaagaat
     7921 aaaagagtta ggacgggatt atctactatt tttctttgtt ttaaatattt gttatctttg
     7981 aacataaaag agaaatgtgt cgactgcagt gttgtgtccc tcatcacgaa taaacggtgt
     8041 tcaattagct gatcttgtat tcacttaaaa aatggtaaag tacgagttgc tattccccat
     8101 tttcaatttt ctaacgctca ggacaaaata ctgttttcac aacctcattg gaataaaagc
     8161 atatatattg tttcaagaac gaaatcttgt tatcttttta aagggcatct gtccaaaatc
     8221 taattttagg aggtacgttt tggtatttat tcgtacaaat aaaagagaat tatctttatt
     8281 catgtaacgc atccaattga ttttttcatg tacgctcctg gaacgatttt actgacgatt
     8341 gcttatttaa ctgcgaatcg ctcattagct tttatcatag ctatccatat tttgaataac
     8401 gttcttggct ttgtattata gatacagatg acaaaaaaag ttcaagtatg cggtgtagaa
     8461 ccaatatcgc atacttgaac ttttttcaat gagtataaat taaaacttct aattttcaat
     8521 tcccatcatc tgatatgccc gaccgtctga acttcttttg aaaaagccaa atgtgattag
     8581 ataacgtctt tgttcgacat aatcgccact agaaaatacg ttcagcagta tcgaattaac
     8641 ctctgtttct gtataagtca ctccatggtc aaagcgtttc aagacttctt tcaataggat
     8701 gtatttcttt tttgttgaac ttggtacttg ttttactttt ccatcattca tgaatgtgtc
     8761 taaaactttc atcttttctt gctccgtata agtataaacc atcttctttc tccttttgat
     8821 tctaagaata ctccatttat gttagaatat tctggatttt ttattaaagc ggttactctt
     8881 aagaaagata ttatcatagt gctctttttt ttactactac ttataggtag atttattcta
     8941 atccattatt ctcccctacc aataattaaa gaaaaaactg gatcagggtg ttcaaattac
     9001 atgctaagaa aaaagaaccc ttgaatacca agggttcttt ctcagtctga taacaatgat
     9061 taacgacgga tttctttgat acttgtgaaa agtcgttaaa gtattgatat aaaaggattt
     9121 ttattacctc tgactacata ttgactaatt tgttaaaaaa tcgtttttca agaatcctgt
     9181 aataccagtg ctttcaccgt ttttttaact ctctaaagaa ctaaaagcaa tcagaggttt
     9241 taccttttca tttgacgtta agtggcgata aattttcgaa gttgtttggt cagtatggcc
     9301 taagcgatct ttcaagactt cccagctaca gtgttccgct actaagcttg ccattgtatg
     9361 acgagctaaa tgagtatgaa atggcttatt aattcctgag cgattcacgc accgtttgac
     9421 gtgatcattt acatactctc gacgataagg aacaccacgt ttactagaat caagatacac
     9481 aaacaaatat gtttcttcta taataaacct gtaatgttgc ctcatctcac ccaaagcctt
     9541 aatcaactgt tgtagctttt ccataactag aggagttact agaactcgtc tagcagaact
     9601 aactgtctta gtagtttcaa ttcgaaaatc cttactctta cctttaactg ccgtttgctt
     9661 atagaacaga atttcatttc ttttgaaatc tatatctcca ggctgtagag caagcgcctc
     9721 gctgactcgg cagccagtgt aaaatagaac atggaaaagt acagacatcg gtaattcctc
     9781 atgaacaatg ccataattca aaaaagttct caattcatcg acagtaagaa acttgtcctc
     9841 aatattattt atggcttctt ttaaatcagt taccgttaac tgatattttg gcattctcac
     9901 agacttcatt ggattttcac ttatcaaatt ctttctttga caatatttaa aaatcatatt
     9961 tagaacttga ataagtgatt gtaaactatt tttggcatag ccaacatttg ttccattaac
    10021 gccacattcc tttaaggtaa aaagcaattc ttgaaggcac tctggtgtca aatcagacaa
    10081 ctttttatca ccaatccatc gaacaataat ttttaattgt tcctctcgct tatcataggt
    10141 tatttccttt tgttcattta acgaacggta atattggaac cattgctcac ctacaacact
    10201 aaacttgtca ttgcttgcaa caattttttt cttaagcctt tgttgtttct ccatttccag
    10261 ataagcgagt tgcgcctcac gttttgaatt aaagccacgt cttgttgttc gatatgcttg
    10321 aaagtaccat gtgatcctac cagtagaact gatttgcttc ttgaatcgat ctgttttttc
    10381 catttattga ttcctcctat tttgaaggaa cccaacaagc agaaaccctt tggaaatctt
    10441 tctatctatg tcatcattgt accgcatctt agaaagaaaa tgtaacttat aaagatcgaa
    10501 gtagtcgcaa tttttctcga tagtcattcg aatcactctt tctcatccat tctaaaacat
    10561 catcacgaaa atagtgtcct cgaatcccgt accaacaggg aggaaactca ccaagtttta
    10621 ctaaacggtt cagtgtacta tcacttatct gaaatagtcg ctttaaatca gtatttgtta
    10681 ataccagagg taaatgactg actgcaagca atttttccgt gtaatcttcc agtttttttt
    10741 gtaaaattga atagaactct atttccagag aattaacatt tgttttaact tccaactcca
    10801 tcacccttct ctctcaaaat caaaacgacc accacggtac cccttaaaat tgtaaaaaaa
    10861 cactccagta ctccatcaaa acggactaga aaagaggaat ttttaatatg aaacaaggaa
    10921 ttgcagacat aaaaataatt aaagaaattc ttgaaaaatc tacggctaat gcaatagcat
    10981 tcggaacagg aatcaacttg agtacagtaa aaaaacttaa aagtggagag agagctgaag
    11041 aaaaattaaa tcttgccgat gctattaaaa tcacagaatt tggcatgaaa aatatgccaa
    11101 ccaaaatcga aatatggaaa tcctagctct tccaaagcca tttgtcgttg gtaccaaaca
    11161 ctgccgagac ttttcccaat aatatcgcca atttcacgat cattaagacc gatgaagaag
    11221 gacataaaaa tgatttcttg tctttgacta ggtaatgctg caatcgcatt tgccaactcc
    11281 tcattggaaa taaagatttt cataccaagg acaataaaag taccactact attcaaattt
    11341 ggttccaaat aatagaactc tgtacctaac cctttttgtt ccaatatttc cattgatatt
    11401 tgccgttggc gaaatctagc gtacttcttt tgaatattta acgcctcatt gcagactact
    11461 ttcttacaaa aactgtcaaa cgtatgttga atgtgggttt cctctgagtc gcccataata
    11521 ttctccccct caaaaaattc attcaactcg ttttacttct atccctttcc attattacta
    11581 caatctagga
//
LOCUS       DACDTO010000091        11320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDTO010000091 DACDTO010000000
VERSION     DACDTO010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.091"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            166..972
                     /locus_tag="IWK70_002384"
     CDS             166..972
                     /locus_tag="IWK70_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9614886.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            984..2204
                     /locus_tag="IWK70_002385"
     CDS             984..2204
                     /locus_tag="IWK70_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9614887.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2194..3780
                     /locus_tag="IWK70_002386"
     CDS             2194..3780
                     /locus_tag="IWK70_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9614888.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3819..5957
                     /locus_tag="IWK70_002387"
     CDS             3819..5957
                     /locus_tag="IWK70_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9614889.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(6325..7308)
                     /locus_tag="IWK70_002388"
     CDS             complement(6325..7308)
                     /locus_tag="IWK70_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9614890.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            7686..9077
                     /locus_tag="IWK70_002389"
     CDS             7686..9077
                     /locus_tag="IWK70_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9614891.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            9093..10133
                     /locus_tag="IWK70_002390"
     CDS             9093..10133
                     /locus_tag="IWK70_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9614892.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            10152..11237
                     /locus_tag="IWK70_002391"
     CDS             10152..11237
                     /locus_tag="IWK70_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9614893.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
BASE COUNT         3853 a         1796 c         2217 g         3454 t
ORIGIN      
        1 ttttggttta ttgcaattgt ctagcatctt ctgtaaaata gaaacgttag agaaaatctt
       61 gtatcataga aaattaagaa agaaataaga acgcagctcg gtaccaaaaa tatggtaaaa
      121 tggcacttga ctgtaattca ctgaaaatcg cttggaggaa cacaaatgtt taaagatttg
      181 tatttattta tgaaggacat ttatcagaac agaaaattat tgctacagtt ttcgatcaat
      241 gacttcaaat caagatatgc aggatcgttt ttaggaatct tatgggcgtt tatcaatccg
      301 ctatttactg tattgattta ttggctggta tttggtgttg gactgaaagc acaaatgatc
      361 gatgcaaaat acccatttat cgtatattta gttaccggga tgattccctg gttcttcttc
      421 tcagatgcat ttgcatcaac tacggttgtt tttcgggaat atacttattt agtaaagaaa
      481 gtcgtattca atattcgtat cttgcccacg acgaaaatcc tatccaatct gtacacccat
      541 ttattcttca ttctgattgg catagctgtc gcgcttataa atggtatata tccttcagca
      601 atgtctttac agctgattta ttatttattt tgcatgtgtg ctttcttgac aggattgact
      661 tggctgacgg catcgatcca accattttta cctgatatca tgcaattaat tacgatcgtg
      721 ctgcagttga tcatgtggac attaccgata ttatggagcc ctaatcagtt tgattcaggt
      781 cttgtaagca tattgaaatt aaatccgctg tactatgttg ttcaaggtta tcgtgagtca
      841 tttttaagtg aagcttggtt ctgggagcat ggaacatata ctttgtattt ctgggttttc
      901 acgttagtaa tgttcgtaat cggctctgca gtgttccgta gattgaaacc acatttttca
      961 gatgtgctgt aaggagaata gctatgacag aatatgcaat tagactaaaa gatatcacaa
     1021 aatcttataa tatgtacgca aaaccttcgg atcgatttcg tgaagccctc aatcctttta
     1081 agaaatccta tcatgatgta ttttatgcgt tgaaaaacgt gaatgtagaa ataaaaaaag
     1141 gtgagatgat cggtttcgtg ggagaaaacg gatcgggaaa atctacaatg ttgaaaatca
     1201 ttacgggagt actgacacca acatccggca cgatggaaat cgaaggaaaa atctcagctt
     1261 tgctagaact aggatcagga ttcaatcctg agtattctgg ctatgaaaat atctatttaa
     1321 atggaatggt tctaggattt tcaagagaag aagtcgatga aatggtcgat gatatcattg
     1381 aatttgctga catcggagac catatttatc aacctgtaaa aacttattca agcgggatgt
     1441 tcgttcgtct agcttttgct gtagcaatca atgtgaatcc cgatattttg attgtggatg
     1501 aagcattggc agtaggcgat cttgaatttc aactaaaatg tatggaaaaa tttacagaaa
     1561 tcaagaactc gggaaagacg attttattcg tttctcatga tatcagttcg attcgtcgtt
     1621 tttgtgatcg ttcctattgg ttgaaaaacg gagaagtcgt cgaatatggc gatacgatgg
     1681 aagtaacgac gaattatgaa aactttttaa agaaaaaatc agttaaaaca gtggatcgga
     1741 ataaaaacca agtagaacgg ttcgccgctc cagatatcgt cgacgtgacg aaagcggaat
     1801 tgttggataa agacaaaaat ccggtggata tgctggaaca aggagaaaaa ctatacgtta
     1861 aagtaactta tgaagtaaaa gatgatacaa tcaaaaatcc ggttttagga attgcgctac
     1921 gaacagtcga caatttttat gtatgcggac ttaatactct actagatggt atcaagattc
     1981 cttggaaaaa aggagagaat atcttttatc tggagtatga aaagttaggt ttgttagctg
     2041 gtgaatatta ttttgacgtt gcggtctttg aagaaaatgc aacggttcct ttggtatata
     2101 agacaaagta catgaacttg tttgtcagcg gttcttatat cggagaaggc atcgtggtct
     2161 tagaccataa atgggaagaa gggactcata gcaatgaaat atgattttga aatggaccta
     2221 gatgaacaaa gcagtgtggg aaaaattgcc gcacagatca aaccaggaag taaagtttta
     2281 gagtttggtc ccggaaacgg ccgattaacg aaacatttaa tcggagcaaa acaatgtgaa
     2341 gtgagcattg ttgagttaga taaggaattg ttcgattttg tcagcgaatt tgctcaagat
     2401 ggattttatg gtgatatcga aagttttgag tgggcaaact attatgctgg acagacattt
     2461 gattacgtct tgtttgcgga tgttttagaa catttggtag atcctggaaa aacattgaag
     2521 aaagtcagag aatttttgaa tgaagaagga gagatcctga ttacattccc taacttggcg
     2581 cataattctg tcatgatcga tttgttcaat aaccaattgc cgtgggcttc ttatggtctc
     2641 ctagatgaaa cccataattc tttttatact catgatggct tccaaaaagt ttttgaaaaa
     2701 gcgggattat tcattaatat tgaagattat ttgtacttag ctgtaggtga tacagaatta
     2761 aaatcaactt atgaagaatt gccggaagct gtacgctatg atttcaaaat gcgaccattt
     2821 ggtgaagtct accaatactt tttctcattg atgaaacatc cagtagcaca aagttccatt
     2881 gcagagccac aaaattctaa ttatgtaaaa gtgttagaag taacccagca aacgaaacaa
     2941 gatgaaacaa tccaacaaat accattcaat aatttcacag gggaaaatga gacactaagt
     3001 ttccccgtat cagaaacaac agaaagaatg gtgtttcgct tcgcgcaaca acctagcttt
     3061 atcgaattca gcgcagaagc tgccggagaa aaactaacat ttatcgattc gaatgctgta
     3121 gtcaaaacag taaatgattg ttatttgttt gatgggaaag aattaccaga gttcgttcta
     3181 acggatattt ctgggaaaga agtgactatc cattgtcact atcgctttat cggggaattg
     3241 acaccaacaa tgaaagaatt gttggaaacg attcgtccca tggctgaagt cacaaagcag
     3301 ctttctgaga aaaatgacga attagaaaga gaaaatcatc atcttcttga ggaaaataca
     3361 agactagacc atacgttaaa aacaaccaca aatcgctatt gcacattgtt ggaaagtgat
     3421 gaatggacaa taaaacatag actaggacga cgtaagaaag aaacttcgaa aaaaatccaa
     3481 gaaaaagaac tttctatatg tattgacgag aaaatctggg atgcagaaac aaaaatatta
     3541 aaaatcatcg gatggggaat cgctaattcg gatcgccagc ccttgtccta taagttatca
     3601 gctgaccaat cgccattttt cgaagcaatt cctgtttcac gagaagaagt caatcaagca
     3661 gaacagctcg caaaaggaac agaagcggga tttgagttac gaatcctttg cgagcaagaa
     3721 cgttcattct tagtggaagc agtagctcaa aatggccaat catggatcat tgaaatgtaa
     3781 ataatgatta acttataaag ataaggagta aacgaatcgt gcaaaaaaaa gaaattgccg
     3841 tatttatcga tcaaatcact cgtgaacggg ccactggcga tttgcttatt gtgggctggg
     3901 caattgatga agtgaccaaa gaaatcccta cgataaaggt agaaaaagaa aatgtgattg
     3961 ctgaagcaac acatgtcgta agattggata tcaatcattt atataatctt gatgttaaga
     4021 cccaaagcgg tttcaaggta cgtttatcag ggaaaatgag aggaaaagcc atcttagact
     4081 tccaaacagc gagacatcaa aatggcatag ctgtgaaatt aaacggtaaa tacccttatg
     4141 atgacggcat tgaatcttca tgggaaagaa aaaaaagatt actgaaaaaa gggataaatt
     4201 atgcaaggac tcatggcgta aaaaaagcga tccgtcgggt caaattggaa ttgaagccag
     4261 gatcgatcga ctatgcagag tggatcagtc gtcatgagaa accagatttg aaagagcaaa
     4321 ggaaagaagc acaaggattt gcgtaccgtc cattgatttc catacttatg cctgtctata
     4381 acgttgaaat caaatggctc gaaaaatgca ttgattcggt actggatcaa acgtatgatc
     4441 attgggaact atgtatcagt gatgatgctt ctactgatcc agctatccga aagtgtctgg
     4501 aatcctatca agcaaaagat gatagaatca aagtcgtttt ccgaaaagaa aatggacaca
     4561 tcagtctggc aactaattct gcattggaaa tagcagaagg agaatttata gcactcttag
     4621 ataatgatga tgaattaccg ccatttgcat tatatgaagt ggcaaaagtg ctaaacgtac
     4681 atccagaact ggatgtgatc tacagtgacg aagataaaat cgatgcagat ggcaatcgct
     4741 ttgatccgca tttcaaagca gactggtcgc ctgatacatt gatgggaaat aattatatct
     4801 ctcacttagg cgtatatcga acaagcattg ttaaagaact tggcggtttc cgtaaaggct
     4861 atgaaggttc tcaagattat gatttggtcc ttcgtgtgac agaacaaatc cctgcagatc
     4921 atatttacca cattgaccga gtcttgtatc attggcggac gatcccagga tcaactgcaa
     4981 gtaatggcga agccaaaagt tatatctatg attccggagt gaaagcattg acagatgccc
     5041 tgtctagaag aaacatcaaa ggaagcgtac gtcctggcag aatttctgga ttttatgaga
     5101 ttgcttatga cgtcttgcaa gaggatttgg tcagtgtgat cattccaacg aagaatggct
     5161 atgaagatct aaaaacttgt gtggattcga tcatcgaaaa aacgtcttat tcaaattatg
     5221 aaatcattat tgcggataac ggaagtacag atccaaaaat gcaagaatta tttgcagaat
     5281 acaagcatca gctgaaagac cgctttatcg tagaattgat tgacattcca ttcaattatt
     5341 ctcgaattaa caatttagca gcagaaaaag caaacggcaa atacttttta ttcttgaata
     5401 acgatacaga agtgattgag ccagattgga tgacggtgat ggtttcttat gcacagtttg
     5461 atcgaatcgg ctgtgtagga gcaaaactgt tttatccaga tgatacaacg cagcatgcag
     5521 gagtattatt aggaatcggt ggtgttgcag ggcatgcatt gaataactat gatcgaacac
     5581 attgtggtta tttcggccga ttggtcattg acgtcaatta cttagcagtc actgcagctt
     5641 gtatgatggt caaggcagct gacttcaatg ctgttaacgg gtttgatgaa acattagaag
     5701 tagcattcaa cgatgttgac ttgtgcttga aagtttatga actgggtcgt tacaatgtct
     5761 atgcacatca agcagaattg taccatttcg aatctaaatc aagaggttat gaagatactc
     5821 cagaaaaaca aagacgtttt gctggtgaaa tcaaaaaaat gcaagataaa tggccagcat
     5881 atattgcgca tgatccattt tacaatgata atttaacaaa agacggaatc ggcgactttt
     5941 cgttgagacc agattaaact ataataaaaa gctgttatgt tgtgattcgc tcgcacataa
     6001 cggcttttta tagttttttg tcaaaaaaag gcagaaggtg ccgactcaaa aaaataagtc
     6061 gaggttggga cagaagtgtt taactccgag aaataagaag aaattcacga aaattgcttt
     6121 tcaaattttt gtgaatttcg gcttatttaa gaagaagttg cttctgctcc caccgtttat
     6181 tcgtttttaa agaatgagac aaaagtgttt cttacttttg tcccacgctc ttattcatgc
     6241 tcagagcgcc aatagataca aaatcatagc caaagtttca cgaaccctag ctgtcaaaga
     6301 atcgatattt tattttaata taggctattt taaaataaat ggtttaaggt attcctctac
     6361 aaaagccact ctatcaatta tcatgggttt aaaataagaa gtgatggaga taaccagatt
     6421 gttcttagcg tcaatataaa gaacatttcc actattacca attgctgaat atatttcgtt
     6481 ctcagaatct aatacccacc ataaatagcc atatctcata ttttgtaagt cttctaaagc
     6541 actggttcta ggtttgaaca tctcgtttat ccacatgggt ggtacgattt gttttccttt
     6601 atacatccct ttatttaggc ataatacacc aatttttgcc agatcacttg ccgataaaca
     6661 caaaccatat cctgccgtcc cgatatttag tggatcacaa aaccagacat tggttttagg
     6721 agacttatcc attgtaaact gcttatgttc ttcagctgtt tcagcataat aattgacatg
     6781 gtttggtatc cctataggat caaatagata cttattggca aaatcaacag ttgttagctt
     6841 acttgtctga tacaacaagc cagttaatat gtgtaaacag acagtttgat agttgaattc
     6901 gccggttata ccttttcttc ctcccaataa atctagtgat gtttttgtcc aatcttcact
     6961 tgaacagact tttgtccatg gatcgccttt acatttgtag ggtgctttca ttgttagcaa
     7021 gtgcttgatt gtaatagcgt aaatagtttt ctccccgcgt ttcactttat actctggaaa
     7081 ataatctaat actttatcat cgatactttt gattaatcct tgctctaaag caatgccaat
     7141 cagcaaagat acaatacttt tcgttgctga catgatatga caagtatcgt cttttttata
     7201 accattccat tcatctgtat aaacttctac cccatcctta tatgcgacaa tttgacatat
     7261 atttggttgt cgaaaaccaa taaaatcatg caactcttgt ttattcataa gatattacct
     7321 cactttcttc tataagcata atgtcattaa aaatcgattt caattttttc gattcttcac
     7381 gtaatacagg tattcttatg gactagacag cgctttggga agatggattc tctgaaaaat
     7441 ataaaggttc actttatgag tggataaaaa aagcagcaaa tacttcgact tctttaattt
     7501 accaacatta gtgaaaatcg attcatctgg agatttgtaa tttagagtcc ttgtcttgat
     7561 tagaggtgta taaagctttt tattagacat ttttaaattc cattacaatt tgcttataaa
     7621 ctaggttttt aaaggtgact atggtaagat aatagagata ttattttgtg attaggagtg
     7681 tatatatgaa taaaaatgga gaatggagtg ctgcacgacg gatattcatt atattaatgg
     7741 atgttattgt atacaatgca gcaatatata gtagtttctt actgaagttt cgtggagaga
     7801 tcccattacg aaatttagaa actttccaac actcagcaat tattatttca gtgattttta
     7861 ttgcgttgaa cattttgttg ggtgcatatg ttttttataa tcgaatgatc agtgacatcg
     7921 tgtttgtcac agtcatcggt caggtattga tgacactagg gatcatggta gtgacttttg
     7981 cgggccgctg gtttgctttt ccacgtcttg ttatcttact ttcatttgtt ctcggtacgc
     8041 ttttcttaag tatctatcgt attctcgtat ataaacttta tttacgcgta agtcgagata
     8101 aaaaggtcgt cattattggg ttagaaaagg atgtcgcacc agcaatccaa aattttggtt
     8161 caaagaaaaa caacaaacat aaagtcgaag cagtcattct tgataactat tatgcaaaca
     8221 tcaaaaatat gattaaagac atcgatattg tctatcttgc ttctcatatc gaagaaactg
     8281 aaaaaatcaa aatctatcaa ttagttacta aaaatgaaaa aaaattgttt ttgaatacaa
     8341 catttgaaaa cttgacattg atcaatccaa atattatgaa tttcgaagat gaaagtatta
     8401 ttgaagcatc tggatttaga atccaacctg aatatgaact attcaaacgt ttgtttgata
     8461 tcatcgtttc acttgtttta ttagttattg cttcaccatt tatgttattc acagcaattt
     8521 tagtcaaagt gacatcaccg ggaccaatca tctataaaca agtgcggatc acaaaaaatc
     8581 aaaaggaatt ttcaatctat aaattccgct cgatgaccgc cacagccgaa gcaaaatctg
     8641 gaccagtttt agctaagagc aatgatgcac gagtaacgcc tgtaggtaaa tttattcgag
     8701 ctgtccgctt tgatgaatta ccacaaatct tcaatgtgct gaaaggcgat atgtcgatcg
     8761 taggaccacg tcctgaacgt tctttcttcg tcgatcagtt caatgcagaa aacccttatt
     8821 actacttacg gcataatgtt cgtgctggta tcactgggta tgcacaagtc tatggaaaat
     8881 atgtctctga ttacaacagt aaattacgtt ttgatttact ttatatcaaa aaatattcat
     8941 tgatgcttga tttaaagatc ttgctgcaaa ccatcaagat cctatttgat aaaatgtcct
     9001 ctcaaggact tgaagaagac gaaaatgtga tctcattggc agaaattgct tttccgaatg
     9061 aaaaaatctt tcgctagttg ggaggcgtaa aaatgaacaa tccgaaattc agcataatcg
     9121 taccagtata caatgcagaa gcaactattg gtcgaacgat tgatattcta aaagaattgg
     9181 attatgaaga gtacgaagtc gttttagtaa atgatggatc aacagatcaa acacaggaaa
     9241 tcattcaaga actgattgaa aatgatgaac gttttgaatt gattactacg accaatcaag
     9301 ggcccggatt agcaagaaac gaaggaataa gacagtcaaa aggagaatat ctcttattct
     9361 tcgatgcaga tgacacgcca gtaaaaacga tcttgtcaga ttatgatcat ttgctagaaa
     9421 agagtccaaa agcggatctt gttgttagtt cgtttgtttt tcgtacaatg gacgagggaa
     9481 gaatcgtttc cgaaaaagaa aacttagtta cagaatgtca ctatctaact cacgaagagt
     9541 ttataaaaga catgtatcag ttaatgaatg aacagttgat gtatgttgta tggaacaaat
     9601 gctatcgtag cgatattctg aaaaataacc aaattctatt taaaacttat aatagttgtg
     9661 aggatcgaat atttaactta cattattatg aatactgtca tcaagtgatc atgaacccaa
     9721 aagtagaata tatttatgaa tttgaaggtg ggaaggggat cactaatcaa taccgaccta
     9781 acaagttcga aacatttaaa gaattctatt cattagcaaa tactgtaaca gacgatatta
     9841 ataaagacgg tatggcggcg cttcttttaa aagggacaac ttcagttatt ttttctattt
     9901 atagtacctc tgcaagaact tcaaaagaga agaaagacga agcaaagcag atattgagtg
     9961 atccagtgat ccgagaagcc aagcaaattg ctaagacgga ttcttcagca aaaaaaataa
    10021 caaagttgct ttacaatatg ccagctccac tatttttatc tgctgtaaaa gttggaagct
    10081 tcgttgaagt gaaaatgcca gggttaatgg ctgctttgaa gcgagtatat tagttaatgg
    10141 aggaaaatat aatgaaaaga attttgcatt ttcagggacg aatgggttta ggtggagcag
    10201 agtctttcat gatgaattta tatcgtaaaa ttgatcgtac aaactatcaa tttgattttt
    10261 tgatttatga agattatgca gatgttcaag actatcattc agagattgaa agattaggag
    10321 gccgtatttt tgttgtacct aatcctaaga aaaatatttt taaatattta atagaagtga
    10381 ataagttact aaaaaaagaa tctttttcta tagttcacaa tcaggtttat tttggcgggg
    10441 gaattaattt atggttagcc aaaaagaatg gaatcagaca gagaattgct catagccatg
    10501 caactgaaga tggaaaatct caaaatattg taatgaatgt attgcgtaaa tttttaacca
    10561 aattgttgtt acaaaatgca acagactacc ttgccgttag tcaagaagct ggtgaaagtc
    10621 tgtttcaaaa ccatcccttt gagatagttc ataatggtat tgatttagag ctttatagta
    10681 aaaattcaga agctaaggtt aataagagaa aagaattgga tatctcaatg agtacttttg
    10741 ttgtgggaaa cattggacga ttagagaaac aaaaaaatca atcttatttg ttagagattt
    10801 ttttagaaat actaaaaatg aattctgatt cttacttgat tttagtaggt gaagggtcac
    10861 ttagaaataa actgaaagcc aaagcagtag aattggatat agagaaaaga gtaaaatttt
    10921 taggagaaag aaacgatatt cccgagttat tatctgtgat ggatgttttt gtaatgcctt
    10981 ctctttatga aggtctgcca atggttggat tagaagcaca agcagcaaaa ttaaaattag
    11041 tcttagcaga tactatttcc aaagatacaa agttaacaaa taatgtggat tttgtatcat
    11101 tagatgaaac accacaagaa tgggcaaaaa aagtaataaa agagccgtat gctaataaaa
    11161 taacacaaga actaaagagt tatgatgttt cgtacactac tgaacaaatg gagagtatat
    11221 atagtagaga tcattaaaaa tgttcaagat attttaggag tgattatttt tgaaaatatg
    11281 ttatttgata ttggcataca atgatattgg caacttaaag
//
LOCUS       DACDTO010000092        10817 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDTO010000092 DACDTO010000000
VERSION     DACDTO010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.092"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWK70_002392"
     CDS             complement(25..144)
                     /locus_tag="IWK70_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9614894.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWK70_002393"
     CDS             complement(159..1343)
                     /locus_tag="IWK70_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9614895.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IWK70_002394"
     CDS             complement(1599..1853)
                     /locus_tag="IWK70_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614896.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IWK70_002395"
     CDS             complement(1878..3020)
                     /locus_tag="IWK70_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9614897.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IWK70_002396"
     CDS             complement(3080..4432)
                     /locus_tag="IWK70_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9614898.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IWK70_002397"
     CDS             complement(4503..4847)
                     /locus_tag="IWK70_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9614899.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IWK70_002398"
     CDS             complement(4857..5231)
                     /locus_tag="IWK70_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9614900.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IWK70_002399"
     CDS             complement(5244..5558)
                     /locus_tag="IWK70_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9614901.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWK70_002400"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWK70_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IWK70_002401"
     CDS             complement(8976..9638)
                     /locus_tag="IWK70_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614902.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IWK70_002402"
     CDS             10255..10485
                     /locus_tag="IWK70_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614903.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="IWK70_002403"
     CDS             10491..10790
                     /locus_tag="IWK70_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614904.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DACDTO010000093        10628 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDTO010000093 DACDTO010000000
VERSION     DACDTO010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10628
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.093"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWK70_002404"
     CDS             23..646
                     /locus_tag="IWK70_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614905.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWK70_002405"
     CDS             1096..2145
                     /locus_tag="IWK70_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9614906.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWK70_002406"
     CDS             2142..3023
                     /locus_tag="IWK70_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9614907.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWK70_002407"
     CDS             3026..3796
                     /locus_tag="IWK70_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9614908.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWK70_002408"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWK70_002408"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9614909.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWK70_002409"
     CDS             5107..6270
                     /locus_tag="IWK70_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9614910.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWK70_002410"
     CDS             6387..6797
                     /locus_tag="IWK70_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9614911.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWK70_002411"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWK70_002411"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9614912.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWK70_002412"
     CDS             9337..9654
                     /locus_tag="IWK70_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614913.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IWK70_002413"
     CDS             9814..10050
                     /locus_tag="IWK70_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9614914.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IWK70_002414"
     CDS             10047..10445
                     /locus_tag="IWK70_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9614915.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3712 a         1772 c         2248 g         2896 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaact
//
LOCUS       DACDTO010000094        10538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDTO010000094 DACDTO010000000
VERSION     DACDTO010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.094"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..756)
                     /locus_tag="IWK70_002415"
     CDS             complement(<1..756)
                     /locus_tag="IWK70_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9614916.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHID"
     gene            902..1705
                     /locus_tag="IWK70_002416"
     CDS             902..1705
                     /locus_tag="IWK70_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9614917.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            1883..2830
                     /locus_tag="IWK70_002417"
     CDS             1883..2830
                     /locus_tag="IWK70_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9614918.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(2898..3191)
                     /locus_tag="IWK70_002418"
     CDS             complement(2898..3191)
                     /locus_tag="IWK70_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9614919.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(3219..3884)
                     /locus_tag="IWK70_002419"
     CDS             complement(3219..3884)
                     /locus_tag="IWK70_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9614920.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(4024..4656)
                     /locus_tag="IWK70_002420"
     CDS             complement(4024..4656)
                     /locus_tag="IWK70_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614921.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(4663..5730)
                     /locus_tag="IWK70_002421"
     CDS             complement(4663..5730)
                     /locus_tag="IWK70_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9614922.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(5727..6458)
                     /locus_tag="IWK70_002422"
     CDS             complement(5727..6458)
                     /locus_tag="IWK70_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9614923.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(6627..7106)
                     /gene="greA"
                     /locus_tag="IWK70_002423"
     CDS             complement(6627..7106)
                     /gene="greA"
                     /locus_tag="IWK70_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9614924.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(7242..8417)
                     /gene="mltG"
                     /locus_tag="IWK70_002424"
     CDS             complement(7242..8417)
                     /gene="mltG"
                     /locus_tag="IWK70_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9614925.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            8621..9790
                     /locus_tag="IWK70_002425"
     CDS             8621..9790
                     /locus_tag="IWK70_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9614926.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(10057..>10538)
                     /locus_tag="IWK70_002426"
     CDS             complement(10057..>10538)
                     /locus_tag="IWK70_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614927.1"
                     /translation="YSSVVNMVKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQL
                     LLKNHQVTEDGQVIYSSFSPNKDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATV
                     LKQGGPFVTVKSESGINKVTIRYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         3245 a         2096 c         1630 g         3567 t
ORIGIN      
        1 atcaatatgt ccgtcgaaaa taattatctt ttcgctatac tgccaaatat ggacatgatg
       61 aacatccttt accccatcga ttgcttcaat ttcttgttcc attttttcat agtcgatagc
      121 aggtgcactt tgtatcaaaa tagcggcagc ttttttgatc accattatcg cttctatcaa
      181 tatatatatc gaaatgatca gtgtaataac tggatcgatc cagtagatat cgaaaaaacg
      241 gatcaaaatt gctccgataa tcacacttac cgaagacagt gtatcactta acaaatgcaa
      301 ataactagat tttatattca aactttcatc tgcttcttgt cgtaataata atactgagaa
      361 caaattcgcg agcaatccaa taactgctac tgttagcatc aaatgactat ttatcttttc
      421 tggtgaagtg aaccgcttga atgcttcaac ggttaaaaac aaggataata tgatcaatat
      481 gaatgagttg atgaaagcac tcaatatttc taaacgctgg tagccatacg tacgtttttt
      541 atttgcttct tttcgggcca ttttttgtgc cccgtaagaa aaaataattg ccaatgtatc
      601 actaaggtta tggaaagcat ctgaaacaag agacaaactc cctgacataa tccctccaat
      661 aaattctgat aaagtgatca ccgcattcaa aaccactact ccaaatagtt tttttgatga
      721 tctctgtggc tgtgtaggca ctattccttc ctccatttta tttatattgg atagtcttta
      781 gacaagaata acattttttt gatatttcac caatcattca gctgtttcct taaagcactt
      841 gtatttcact tgatacttcg ctagaattgc aagtgatgac actactagat gaggagtact
      901 tatgacaaca aaacaattaa ttatcagttc ctttatacga ttcaaagacc taaaggaggc
      961 gagaaatgtc ccattttgga aagccattgt ctatctttta gttttgagta taattatggc
     1021 tttgccaatc tcttttcaag tttttcaagt cttaaacaac attcaattgg atgggcaaaa
     1081 gattgctact cgcattcctg attttacaat ccaaaacggg cagcttcaaa ccgaagaaaa
     1141 agaaggattc atttaccaaa caaactccat tatcttcaca tttgatccag agggaaaacg
     1201 aacagaacag gacatctcta ctgatttgat ggggaatttt gttagtgtcg ggatgttaaa
     1261 aaacaaactt atcatagctc ttcctaatac aggcacgact tctgctttgt tgaacaataa
     1321 ccaattagaa cttccctaca ccaatgaatc tttaaaaaac ttaacaggta agcagctccg
     1381 ttctttttta agtgaagcat ctataccact ttggataaaa gtcctgacat ttatattttc
     1441 tatttatcct agttttctga atctgctgat cactttgcta tttaccaaca ttgctgcttt
     1501 tctctatgca cgctttcgac taacaaaagc tacttttctt gattgtttaa aaacattgat
     1561 ctattcaatt tcattgccta ctataattgc aacaatcttg atggtctttc ttccttcttt
     1621 tgacactagc gccttcattg cttttgcagg aatctttatt tttgctcaag ctgtcaaagg
     1681 atgggcaaaa atatcgatca attaagaaat aaagacaact cgttatttct acttcaatag
     1741 acccttttag aggttgtgac atttttttgt cacagcccct tttttatttg aaaaatattt
     1801 ctttctcaaa aaacaagcaa ggcgcttcaa agttcttctt gtatgcgcta cacttagagt
     1861 aaagaaaggc gggaaattaa ctatgtcaga aagaatcatt tatgcagatc gtccatttcg
     1921 tgatgaattt atcgaagaaa tccatactat agcgcctggt tatatcttta aaacagaatt
     1981 acaaccagag gatctacaaa atgtagagat cagtttgggc tggaactcag actaccaaga
     2041 aaaactatta gcctcttcta ccttgaaatg ggtccaatct atctcagctg gtgttgatca
     2101 gctacccctt gagacttttt caaagaaaca tattttactt tcaaatggta gtggcatcca
     2161 cagtgaatcg attacggagc atgtcttagg aatcatcctt gggtattctc gtggtctttt
     2221 tcaagcacaa aaggcacaga gtgacatgaa atggttaggt tcatcggtcc attaccaatc
     2281 tatccaaggg aaaaaaatac taatcattgg aactggccat attggaaaaa tgattgcaaa
     2341 aaaatgcaaa gcattagaca tgaactgcta tggaatcaat acatccggtc atccagcaga
     2401 aggaatggaa aagacttttt ctttaaatca actaaaggaa gttcttcctg aagtagattt
     2461 cgtagtcaat atcttacctc taacggagga aacaactggg ctctttgatc agcatatgtt
     2521 tgaacacttt gatccaaaat ctgtattcat caatgttggc cgtggtgctt ctgttaagac
     2581 acaagatctg gtgcaggctt taaataacca gcaactttcc tttgcagcat tagacgtttt
     2641 tgaggaagag cctcttccac aagatcatcc tctttggaaa atggacaatg tactgattac
     2701 ttcccacatt gcaggtttga cacctgattt tcaaaagaaa ttaatggcta tttttttaac
     2761 caatctgaaa agctactttg ccacccatga gcttcaaacc aatcaagtaa aattgtctgc
     2821 aggatattaa tagctgaaaa caatattttc aaaagaaaaa agagaaggtg acaaaaattg
     2881 tgtcaccctc tcttttttta ttgtttttta aagtgatagg ttggctcctc caggttggga
     2941 tcataatcaa caagtaaatc atatccttta aagaaccatt catctgcttc ctcgataaaa
     3001 aaaagtgtcc catccatttc cactttcttt acaggatgtt ctggctgttc aactgacatt
     3061 ccaatcgaaa aaccatcatg aacctctgtt ttgccgtaga cttttccaaa aaaacgtatt
     3121 ccgtaagttt ctggcagatc aatttctgag tcaaaccact tttgtgcttt tggcgttacc
     3181 gttaatttca tcccaacaac ctactttctc ttttctttct attgattcat cttatcgtct
     3241 agaatatcca catcgcctgt ctctccaata accagtagat tatcattttc acgaacatat
     3301 tcatctgctg caggagaagc gataaccgtt tgatctactc ttcgtatggc gactactgtt
     3361 aagtcaaaac gttgcctgaa atttaattca gctaatgttt tgttgaaaaa tttaggattg
     3421 gagactcgga tttctgctaa agaatattta tctgacaact ctaggtaatc taaaatattc
     3481 tttgacacta aattatgcgc aatccttatt cccatgtctc gctcgggatg gacgacgcga
     3541 tcagctccga tcttttcaag tactctggca tgatactcat tttgggcttt tgctaaaata
     3601 tttggtacgc ccatttcctt taccatcaga gtaactaaga tactggcttg aatatcttct
     3661 ccaatagcga caattacatg atcgaaattt cttatgccta atgatcgaag tgtcatttca
     3721 tcctgtgcat ttgccacaac agcatgagtg gctatattca tatattcatt gacccgatct
     3781 tcattactat caatagccag tacttcttga ccagcttcga tcagtgttcg gcagatactt
     3841 ccgccaaatc gtccaagacc tacaattgca aaattttgtt tcaccgtttt cctcctttag
     3901 tatcttcttt tctcagtata acaaagcctt tacaaaaaca aaagcaagca tcattattgt
     3961 aaaaaagcca agaaaaataa tagttatttt ctcagctttt gattatcctc tttatcatcg
     4021 tttttatttc accaaaccat gtttgaatgc atagatagcg gcttgtgtac ggtcttccac
     4081 ttctagctta gccaatatat tggaaacatg tgtttttact gttttcagtg taataaacaa
     4141 ttcgtccgct atttcctgat tactttttcc ttcagaaatc agcattaaaa tttcattttc
     4201 ccgattcgtt agttcttcat gtaatacagg ctcatttcgc cgactcattt tttccatcat
     4261 ttttgttgtc acttctggtt ctaatactcg ctcgccacgt tgcgttgccc ttatagcatc
     4321 agcaatttca tgtgcggtag aggtcttcaa caaataccct gctgcacctg cttcaatggc
     4381 aggataaact ttttcgtcat caataaaact ggttacgata atgattttag cttctggcca
     4441 atcttttaag attgcttttg ttgattcgat accatccatt tcttccatga ctaagtccat
     4501 taaaatgacg tccggtcgaa gtgtcatcgc cttctcatag ccttgtcgtc cattttcagc
     4561 ttctccaatt acttcaatgt cttcttgaat agataaataa gaagagacgc ctaagcgcac
     4621 catttcatgg tcatctacta ataaaacttt tatcatcctt atctatgcct ccttcatcaa
     4681 agggaccttg atttctacac ttgtaccttg acctttaaaa ctaatgattt ttactgttcc
     4741 accaatacca gctactcgtt ctttgatatt gttcaatcca taacttcccg ttttcgtttc
     4801 attcatatta aaccctgttc cgtcgtcaat aatacgtaac aaaagattat tgtctatttt
     4861 gtgcaaatat acctctaatt cattggcttt agcatgtctt aatgtatttg ataacaattc
     4921 ttgaacgatt cggaacaagt gatcctcaat ggaactagat agttttacat cttctacatc
     4981 ccatttcagc gaaatctgga ttttattttg caattccttc aataactgct cgataccttg
     5041 ctttaaactt ttctcttcta aattgactgg acgtagatga agaagcagcg cacgcatctc
     5101 agactgggaa gcgttgatga tctctgctac cattttcaac tgcttgcgaa acatttctgg
     5161 tgtctcgctt ttttctgctt gctccgtcaa tgccgacatc atcatcattg ctgcaaacaa
     5221 ttgttgcgaa actgaatcat gcagctcacg ggctaatcga tgtctttcta attctaaaat
     5281 ttcttctttg gtttgtccat ctacatattg gggacggctc gttacaattt gtaattcact
     5341 agatacttca atcatttttt cttttatctt agtgatttcc ttatcaatat ctttgatgta
     5401 taaatcgtcc gatgcacttg ggatcggaag aagcaacaat ttggattcat aattgccaga
     5461 agaaagtgca cgcagctttt cttcgatttt cccgtatttt gtcttttgaa taagtgataa
     5521 taatagaaag acaattaatc ctacacctaa ggaaatggct aacaaatgga agataagcgg
     5581 aacatataat aaacgtgcac gcagtaattc tccccaccaa tgactttgat tgctggcata
     5641 aaaataagtg aaaagtgaaa atagaataat aaggaaagcc gcaatccccg aataaacagc
     5701 tagcatcgct ctggatattt ttcccatcat attcgtatca cctcgacatc tccaatcaag
     5761 gtattagtaa taatcttcaa tctacgaggg ttttcatcat aatcactgct gtagattttg
     5821 atttgttcat ttttcaaaga aaattgctct tcttcaaaca agacatttcc tactaatgtc
     5881 gcatgctcta atcggatggc tacgcccaaa ggtactaaga tccgtgttct gcctatccct
     5941 tttcttacaa tcacaatatt atcatctttt ggcaaaagcg tattgcccaa atctatgata
     6001 gtatcaccag aaatgacggc gatgttgata tcgtcccatt catagacgtc gtttccaata
     6061 cgttggttac cgaataattg ctgtcttttc cgctcattat tatgtgtttt taattgctct
     6121 gtttggatca tcatgatctg tttctttcgc caaaaagcat ttttggttaa atctatccct
     6181 gaaatctcta cacctttcag accaataaat aaaacggcaa atacagccat catccataaa
     6241 gcaggactgt tgactaagct aaaaaaaatg accaaacttc ctaatatcaa ttgaaaattt
     6301 tgaaatttag tacgaggatg tttgcgcata gctagatata tattgaaaat accgaatatc
     6361 actaaaagca aaagtccagt attgtttacg atttgccaaa cagcaaataa cagcagtagt
     6421 gcttcaacta ccacaaaaaa acgccaagaa ctattcatcg ctctttctcc ctcctgacac
     6481 attctcattt catcatacct atttttttaa ctgttggcta taggacttta gatgtaagat
     6541 cgtttgttga aatctaaaat acacaaagag aaaaagatct agagtgtatc atcagtaaga
     6601 tgaactctct agatctttca gtcagactac gcttgttcta ctttgacgat tttaactaac
     6661 atatcgccac ctggagtagc aatagttact tcatcatttt tacgtttgcc aatcaatgct
     6721 tgcgcaatag gagaatcatt ggaaattttg ccagaaaacg gatctgcttc agcgcttccg
     6781 acaatcgtgt actcttcttc ttctccatct ggtagttcga tgaatgtaac tgttttacca
     6841 attgatacct catctgcatc tacaccgtcg ttatcgatga tttgagcaaa acgaatcata
     6901 ttttcaattg ttgtgattct tccttcaaca aatgcttgtt catctttcgc tgattcatat
     6961 tcagagtttt cggaaagatc tccgaaaccg cgggcaattt tgattcgttc aatgatttct
     7021 ccgcgtttga ctgtttttag ttcttctaat tctttttcta atttttcttt accttctaga
     7081 gtcattggat aaactttctc taccatgtgt aacgtctcct tttttcgata aaataccgca
     7141 aaaggtaatc ttttgaggaa tatagtccaa aaaagattac cacgcttttc ttcaaatgta
     7201 aatgtttttc taaaaatgat caatgtctag agcctgtttt tctaagaatt atttacatat
     7261 ttttccacta attccatatg ttcttcataa gttttagcga aataaacatt tccagtgctc
     7321 gtatctgcta cgaaataata atagtcattt tctgctggtt ctaatactgc cttgattgcc
     7381 tcttcgcttg gattgtcaaa aggtccaggt ccatagccgg tattggtata caaattatat
     7441 ggtgaatcta cagctgtatc ttcatacgtc actaattctt tgtgttcacc taatgcgtat
     7501 aaaacagaga tatctgactg aagtggcata tttgctttga ttcggttaaa gaagacttgt
     7561 gcaatgtttt tacgatcatt ttcttttacg ccttcttttt caactagaga agctagagtt
     7621 aaaacttctt gtacagtcaa atttttctgc ttgatagctg aataatagtt ttgcatgacc
     7681 gtattcgtct tgttcaccat ttcaatcact agatccttaa gcgaagtact cgtgtaataa
     7741 tcatatgtgg ctgggaataa gtaaccttcc aaacggtatt ttgtgttctc agctttagat
     7801 gcactttcca acagttctgg gaatttttgg tgcaactcgt taaagaaagt ctcatcattc
     7861 actaaatcta aaaattcttt cttgtcttta ccagttacct tcgctacccg ttcagcaatc
     7921 tgatcgatat cgtacccttc tggaatggca attttcccat ctgcaacttt tgttggctca
     7981 gaagtaccgc cttcttgcaa ctgtttgcca atctcgtcaa gagtcatatt aggcgctaac
     8041 tgataatagc cagcttgaaa gccagttaaa tttttaaatt ttgtataata attgaaaaca
     8101 atcccgcttt taataatatt gtccttctcc aaaatctcac caatctgctt gttagaagag
     8161 ccactcggga tctcgacttg gactaattgg tcatctgatt tatctaaagg cttcaacccg
     8221 ctatctacat aacgatacac ggtaaatcca agaaatccgc caataatcaa taatgtaaga
     8281 gcaatcacta aaatgatctt cctcacaatc ttatcttctt ttttacgcag atttctccgc
     8341 ttgtcgatta tatctgctgt agctgcctta ctggcttctt ttttatcttt acttgggtgt
     8401 tgtttgtttc ctgacaaaac aactcctcct tactatcaag ttcgtaactt tattcattat
     8461 acatgatatt tgaattggcg tgaatgattt tatttaaata ttctatcaaa aatgtaagta
     8521 gtaaatccat tactataaga ttaaaataaa tgttatcaag atacaagcta ttttagcttt
     8581 attctttctg taaaaaagaa aggagactaa ataaactatt atgaaaaaag gaaaacatgc
     8641 taaaaaaaga aaatattcat ttttaatgat cgttgtcgtg ttgtttagcc ttagtgcgtt
     8701 ctctacagga actattcttg gatggcaatt ttacaataat gaacactctc agatagttgc
     8761 ccatgctcaa tcagctcaaa aaaagttgat actgactacc aatgaacaac agctcgttga
     8821 aaaaatcgat caactattaa gaaaaaatga ttatattggc tcaatttacg taaagaaaaa
     8881 taatcgcatc atcatggaaa aaggctatgg ctatgcgaat aaactcacgc aacaaccaaa
     8941 tgatccttct ctttattatc agattggttc ggttcaaaaa gcattgacta ctcttttgat
     9001 tttaaaacaa gtcgagcgtg ggcacctttc tttagatact aagctggctc aattctatcc
     9061 acaaattgct ggcagccagc aaattactat ccgtgacttg ctgtatatgc gctcaggctt
     9121 gcaccgaact gcttctccaa ccataccgat gactgatgaa gaagtcgttc aatttgcgat
     9181 tcagcactta aaatttacag attatcagac ctctcattat gattcattga actttaccat
     9241 attgactgga attttgatca aattaacaaa tcaaccgtat gaaaaattat tgaccgatga
     9301 tatcatcgaa cctttaggat tacagcatac aaaattttat gaccaagtca aaaattcatc
     9361 caatcatgca ctatcctatc aaatgtctaa agaggatgat tactttaaac cactgacaga
     9421 atctgaaaca gagatccata acgaattagg gactggtaat atcagtatgt ccgtctatga
     9481 tttaaatacg ctgtttacac aagttttatc tggaaaacta gtttccaaag acttactctt
     9541 ctcattatgg cgtgaaaatg cgaatggaca tccatatagt ggtggggtat atagcggaga
     9601 taattatatc ctgtcgcaag gaaatatcga ctcttttgat acatctgcta tatttagcaa
     9661 agattcacag gatgctatca tcatggaaag caatataaaa gctgacaaaa ctatcaaact
     9721 gcctactgct gatctaagaa accaaatcta tgacctgatg gaaggaacta ctaagttggc
     9781 aaatagctga tatgatgaaa agatagcaaa tactacttct ttgaatagca tttgcttaaa
     9841 aaaagtactc gactaaacgt aggaaagagt gtgggacaaa tctaaaaatc aatttttgct
     9901 acagacttta attccaaata aacagaacag aaagaggtgt tcttctttct gttctgtcca
     9961 aattattcta agatttctct ttaccaacat tatttgacaa agggcctatt aaatctaaaa
    10021 tagtatcttt agattgctaa aatatgtgct agcaaatcat tcatggcttg ttcttatttt
    10081 taattttatg caataaaaaa gaagaacaat tagcgtgatt atagaaataa ataatgagat
    10141 tttagaaaaa aatgtgtatg aatacctgat tgttacttta tttattccac tttcactttt
    10201 tactgtaaca aaaggacctc cttgttttag tactgtagca ttttttgaat taacagtaac
    10261 cgttaatcct ttataccgga atacaggtaa aactattgtt ttgccttttg aagatttaaa
    10321 cgtaaatgta atttcatctt tatttggaga aaagctacta taaataactt gaccatcttc
    10381 tgtaacttga tggtttttca aaagcaattg atctttttta tagcctatag gtacataatc
    10441 aaaattctca aattcttcaa gtttattttc catttgttca ttagaataag ttccttgaaa
    10501 attcatttct ttcaccatgt taactactga tgaataat
//
LOCUS       DACDTO010000095        10193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDTO010000095 DACDTO010000000
VERSION     DACDTO010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.095"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            121..627
                     /locus_tag="IWK70_002427"
     CDS             121..627
                     /locus_tag="IWK70_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9614928.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            644..1543
                     /locus_tag="IWK70_002428"
     CDS             644..1543
                     /locus_tag="IWK70_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9614929.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1630..2514
                     /locus_tag="IWK70_002429"
     CDS             1630..2514
                     /locus_tag="IWK70_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9614930.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2706..2777)
                     /locus_tag="IWK70_002430"
     tRNA            complement(2706..2777)
                     /locus_tag="IWK70_002430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2742..2744),aa:Arg,seq:ccg)
     gene            2837..4171
                     /gene="rpoN"
                     /locus_tag="IWK70_002431"
     CDS             2837..4171
                     /gene="rpoN"
                     /locus_tag="IWK70_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9614931.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4374..5411
                     /locus_tag="IWK70_002432"
     CDS             4374..5411
                     /locus_tag="IWK70_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9614932.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            5452..6453
                     /gene="gap"
                     /locus_tag="IWK70_002433"
     CDS             5452..6453
                     /gene="gap"
                     /locus_tag="IWK70_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9614933.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6563..7753
                     /locus_tag="IWK70_002434"
     CDS             6563..7753
                     /locus_tag="IWK70_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9614934.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7857..8612
                     /locus_tag="IWK70_002435"
     CDS             7857..8612
                     /locus_tag="IWK70_002435"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9614935.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8728..10026
                     /gene="eno"
                     /locus_tag="IWK70_002436"
     CDS             8728..10026
                     /gene="eno"
                     /locus_tag="IWK70_002436"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9614936.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2111 g         2631 t
ORIGIN      
        1 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
       61 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
      121 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
      181 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
      241 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
      301 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
      361 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
      421 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
      481 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
      541 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
      601 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
      661 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
      721 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
      781 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
      841 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
      901 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
      961 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
     1021 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
     1081 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
     1141 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
     1201 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
     1261 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
     1321 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
     1381 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
     1441 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
     1501 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
     1561 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
     1621 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
     1681 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
     1741 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
     1801 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
     1861 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
     1921 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
     1981 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
     2041 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
     2101 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
     2161 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
     2221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
     2281 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
     2341 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
     2401 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
     2461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
     2521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
     2581 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
     2641 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
     2701 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
     2761 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
     2821 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
     2881 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
     2941 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
     3001 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
     3061 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
     3121 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
     3181 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
     3241 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
     3301 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
     3361 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
     3421 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
     3481 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
     3541 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
     3601 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
     3661 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
     3721 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
     3781 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
     3841 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
     3901 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
     3961 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
     4021 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
     4081 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
     4141 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
     4201 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
     4261 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
     4321 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
     4381 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
     4441 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
     4501 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
     4561 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
     4621 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
     4681 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
     4741 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
     4801 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
     4861 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
     4921 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
     4981 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
     5041 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
     5101 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
     5161 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
     5221 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
     5281 tcatggcaat cgcgggcgga aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg
     5341 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
     5401 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
     5461 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
     5521 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
     5581 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
     5641 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
     5701 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
     5761 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
     5821 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
     5881 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
     5941 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
     6001 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
     6061 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
     6121 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
     6181 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
     6241 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
     6301 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
     6361 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
     6421 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
     6481 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
     6541 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
     6601 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
     6661 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
     6721 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
     6781 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
     6841 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
     6901 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
     6961 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
     7021 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
     7081 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
     7141 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
     7201 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
     7261 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
     7321 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
     7381 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
     7441 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
     7501 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
     7561 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
     7621 agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
     7681 aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
     7741 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa
     7801 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
     7861 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
     7921 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
     7981 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
     8041 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
     8101 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
     8161 tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga
     8221 caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat
     8281 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca
     8341 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
     8401 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
     8461 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
     8521 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
     8581 cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
     8641 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
     8701 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
     8761 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
     8821 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
     8881 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
     8941 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
     9001 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
     9061 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
     9121 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
     9181 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
     9241 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
     9301 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
     9361 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
     9421 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
     9481 gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg
     9541 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta
     9601 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
     9661 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
     9721 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
     9781 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
     9841 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa
     9901 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
     9961 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    10021 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    10081 acttagttaa gtaatcgaac tctctacttg ctgaaatatc ggctctttgt caataaggac
    10141 tgatgagttg tgcaaaatca aatctgagtc agaatgaacc acattctggc tca
//
LOCUS       DACDTO010000096        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDTO010000096 DACDTO010000000
VERSION     DACDTO010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.096"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IWK70_002437"
     CDS             complement(247..759)
                     /locus_tag="IWK70_002437"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9614937.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWK70_002438"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWK70_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9614938.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IWK70_002439"
     CDS             complement(3212..3646)
                     /locus_tag="IWK70_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9614939.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IWK70_002440"
     CDS             complement(3656..4444)
                     /locus_tag="IWK70_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9614940.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IWK70_002441"
     CDS             complement(4441..5382)
                     /locus_tag="IWK70_002441"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9614941.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IWK70_002442"
     CDS             complement(5559..6146)
                     /locus_tag="IWK70_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9614942.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWK70_002443"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWK70_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9614943.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IWK70_002444"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IWK70_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9614944.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IWK70_002445"
     CDS             complement(9063..9998)
                     /locus_tag="IWK70_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9614945.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACDTO010000097         9676 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDTO010000097 DACDTO010000000
VERSION     DACDTO010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.097"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWK70_002446"
     CDS             99..293
                     /locus_tag="IWK70_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614946.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="IWK70_002447"
     CDS             562..861
                     /locus_tag="IWK70_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614947.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="IWK70_002448"
     CDS             complement(958..1392)
                     /locus_tag="IWK70_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9614948.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="IWK70_002449"
     CDS             complement(1624..2334)
                     /locus_tag="IWK70_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9614949.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="IWK70_002450"
     CDS             complement(2324..3184)
                     /locus_tag="IWK70_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9614950.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="IWK70_002451"
     CDS             complement(3189..3827)
                     /locus_tag="IWK70_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9614951.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="IWK70_002452"
     CDS             complement(3842..4141)
                     /locus_tag="IWK70_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9614952.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="IWK70_002453"
     CDS             complement(4184..5668)
                     /locus_tag="IWK70_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9614953.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="IWK70_002454"
     CDS             complement(5688..6149)
                     /locus_tag="IWK70_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9614954.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWK70_002455"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWK70_002455"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9614955.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="IWK70_002456"
     CDS             7452..8222
                     /locus_tag="IWK70_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9614956.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="IWK70_002457"
     CDS             complement(8495..9322)
                     /locus_tag="IWK70_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9614957.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT         3013 a         1967 c         1508 g         3188 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatt
//
LOCUS       DACDTO010000098         9495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDTO010000098 DACDTO010000000
VERSION     DACDTO010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.098"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            183..1418
                     /locus_tag="IWK70_002458"
     CDS             183..1418
                     /locus_tag="IWK70_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9614958.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(2417..2677)
                     /locus_tag="IWK70_002459"
     CDS             complement(2417..2677)
                     /locus_tag="IWK70_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9614959.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2704..4005)
                     /locus_tag="IWK70_002460"
     CDS             complement(2704..4005)
                     /locus_tag="IWK70_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9614960.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(4297..4755)
                     /locus_tag="IWK70_002461"
     CDS             complement(4297..4755)
                     /locus_tag="IWK70_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9614961.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(4768..6093)
                     /locus_tag="IWK70_002462"
     CDS             complement(4768..6093)
                     /locus_tag="IWK70_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9614962.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(6110..7522)
                     /locus_tag="IWK70_002463"
     CDS             complement(6110..7522)
                     /locus_tag="IWK70_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9614963.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(7539..8303)
                     /locus_tag="IWK70_002464"
     CDS             complement(7539..8303)
                     /locus_tag="IWK70_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9614964.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(8407..9282)
                     /locus_tag="IWK70_002465"
     CDS             complement(8407..9282)
                     /locus_tag="IWK70_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9614965.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
BASE COUNT         3080 a         1974 c         1438 g         3003 t
ORIGIN      
        1 attgttgcgt gtttttttga aaattggagt ttcaacttga ttacaaaatt taaatttaga
       61 agttgtgaaa acagcgagag acagtttaat attcaaaata tacataggct actcgaacgc
      121 tgtgtttttt agtatactag taagaaacga agggatagac tcattccaat aaggaggatt
      181 tcatgaagac aattcttttt gctcctgcag tattcaattt agctgagaca acacgaatga
      241 ttgaggttgc caaagctatg aaagaccgtt attactgtgt attctttggc ttttctaaaa
      301 cttttgctcc tttgatcaca gaaagtggct ttgagtatca tttactagcg ccgatcctta
      361 cgaaaaaaca agaagaacaa atcataaaat ttgaccagtt gcgtacactt aaaaaccctt
      421 ttaccaaaga aatggttgcc tctcgtatca ctagtgaaca gagtctgatt gctaagtttg
      481 agcctcgact gattgtgaca ggttcgaatg tgacaatttt tttatcggcc cgcaataaaa
      541 agattccttt agtatatgtt aaaccctatg ctttgagtga accttttttt caatcagagc
      601 atgcgctgat gccccacgag ttacgtttct tcggcccttt aagcaatccc ctttggcact
      661 ggacaaaaaa gcgtatagta aatacccgat ggatccccaa tgccttcaaa cacgctgcac
      721 aaaaagaaat gcttccttta ccaaaaacaa gtttacaaat gatggatgct gatctaaatc
      781 tcatcacaac gcctaaacta tttttggaag aagaacagct gccggaaaat tatcgaatcg
      841 ttggtccaat ttttgctaag ctcagtactt ccttgcctga agaagtgcta acttttatac
      901 agcgaaagca acaagaaaga aaacgtatta tctatttcgc aatgggtagt tcaggcaatc
      961 cgcgtatgtt acgcaaaatt ttgttcttct tacaaaaaca accggatcta gcggttattg
     1021 cacctattga ctatctgctt gatgacattc aatctgactc agaagatctt ttaataacag
     1081 aatatctgtc tagtcatctt ttgcatgaat tcattgattt ttcttttatc cacggcgggg
     1141 aagggaccgt ccaaactgcc tgtgaatccg gcaaaccgtt tatcggtgtg gggatgcaat
     1201 ttgagcaaag ctgtaatatt gactattgtg taaaatacgg aaacgcaatc gccctcacaa
     1261 aaccagtgac tgaaagaaaa ctgcagattg ctctgcgcga agtttcttca cccaagattc
     1321 gtcagcaggc acgcacgctg caagccgttt ttccaataaa tggtccacaa caagcaattc
     1381 aagaaatcga aaccttcctg agcaaccacc caatatagtt tcaagtagaa aaagcttttt
     1441 taatagagac tcatcagtaa tttcgctatt cgagacccag acagtgaaaa tacaaaaaaa
     1501 gtaaatagtg gattgaaaag ttagagttac ttaaattacg gtgtctaaaa aacacaaaat
     1561 catccttaaa ataaaagatc tgacgctatc caaatgagca taggtagcgt ctgccattta
     1621 ttcttagtca aattctttga cacctttctc tggttggatc atctcaacgt ttcttcaaaa
     1681 gaaagaccat aataattagg caaatacatt ttcttaaaat gtgtcatata gatctagaag
     1741 catcgctttg tagagatttg tgccgctagt cccctcaaca attactaaat caaatgtagg
     1801 atttaccact ttatctttga taatcaacct ccctcttctg ttgagacaat gatctcgcaa
     1861 tccgatgcat actcctatat aaactgccag caatccccac atattgagta gatcatgtca
     1921 gaatgttcta tgtcgtcagc aacgatacac taaaagtcac gttcaccatg actgattgct
     1981 attcccagca caattatttc tatacacgtt tcatgatttg aataaacact cacatcatac
     2041 atattaccat tcatacacca tactgcagcc cctactatat ggttgtaagc tcccaaatct
     2101 atatttgact tgatcacttt ggtagtagat ctactaattc taaatccatt gctgataaaa
     2161 tttccatttt ttcctccttc tttctgccaa aacagttaaa acaataataa tctttatgta
     2221 aaaaagggct gtgacaaaag ccttgtcaca gtcccatatt ccgaataaac ggtggtcaaa
     2281 agcttgcatc tgcggtaata agttcaacca aacgaaaaat aagccaacat ttcacaaaaa
     2341 tttaagaagc aattttcgta aatttcttct tatttcttga agctaaccac ttctgttaca
     2401 accactttta gaacatttaa agcttcgatg tgacaaacca tctatgctgc tcgatcataa
     2461 aattgatagc aaaaattcca caaagtacat atgtccccca tgaaaacaaa acattcatat
     2521 gcggtacaat gatctttgga agaatcacga tcaaaaatga tgccagtgcc caaatccatc
     2581 gattgatttg aatatgatat gtagctagca tattcaatat aaggtgacta gccgttaata
     2641 aaaagataaa aacaaaaaat aaaatcagcc aatacattgt ttcccacctt ctaaaaacca
     2701 aacttagaca gtctggactt cgttttctcc acgactttgt tcatctgaca gcatcttttt
     2761 atcataagct tttacaaatg gataatagat aataaatgct atcaatgtgt tgatcatgat
     2821 caagataaca acacgccagt catttcctgc tgaaagataa ccgccgattg gagctggtaa
     2881 tgtccatgtt ggcaaaacag taaatctatt gaccatttct aatttgactg caaaataact
     2941 gataatagtc aaaactaaag gaactaagtt gaacgggatg aacatgtaag gattcaacat
     3001 cataggagct ccaaaaatta tgggctcatt gatattgaag ataccaggaa taatagaaaa
     3061 acgaccaacc tctttcaaga attttgattt acagaagaat ataaacaaaa cagataccac
     3121 gatcgttgct cctgcaccac caattgttac cgtccattga taaaactgtt caggagcgat
     3181 atagggtaat tgggtggcag ctgtaccatc tgccaatgcc tttgcattct catccaacat
     3241 gatcagccac attggacgaa caacagcccc tacaatactc atgccatgga taccaaatga
     3301 ccacaacaaa tggatcaaaa acattggtag taaaacacca agcaaattat tccctaaaat
     3361 attggttaaa ggctcgaata acgataataa ggcagcgttt acatcaaagc ccgcgacgct
     3421 tcgaatcagc caagtaattg ttaaaatgac tgcacctgga atcaatgcct caaaactacg
     3481 ggcaacagca ggtggcactt gttctggcat tttgatagta atatttcgat ttttaaagaa
     3541 acgatatact tccactgcaa aaatcataga taagatcccc gcaaacatcc cagatgctcc
     3601 caaagaactc ataggtaaga cccagccaag aggtgcctct tcaggagtta aaacgtttac
     3661 aggaacttgt aacataaaga acgttcctaa agataatata ccacctgtca cactatctaa
     3721 tccatatgat ttagctagat ccgaacccat aacaaaagtt gcataaatag ccatcaatcc
     3781 catcgtcatc ctaaagggta aaacaatcgt tgccgaataa ggggcaataa attcattcca
     3841 tccaggaaac ggtaaattga taaagaccat aaaaaatgtc ccaatcaaaa tcaatgacaa
     3901 tgttgaaaca accccattac gaattgctaa taaatgacgc tgtgtaccaa tttttgccat
     3961 tggtgggaca agtttctctt cgacccattt catcagcttt tccactaaaa ccactccttt
     4021 tcaataaaat atgaaccatt cattgcatga caacgcaaag aatgttcata actaattata
     4081 taattaagaa attttatttc aatatattct cttaaataat caaatttatt tttaagaatc
     4141 ctgaaaataa gttttctaaa tcataggagc aacaaaaaaa ttcgataatt aacaaaaatc
     4201 aaaatgatta aatctagtga ttgaaacata tcatttcaaa aaaattttgt aaattcaccc
     4261 ataaatatca ccctatttaa cttttttcat cgtacactaa aattcaaaac aactatcttt
     4321 aatctttact tcttggtttt gttttaataa atttccttca aaaatgcctg gagaaactac
     4381 aatgatcacc gtatcttttt gtgcttgctc caacttcttc aaatctataa tagctaacag
     4441 tgtcccttct tttacctcct ggttactttc aacccgtatc tgaaacggac ttccttcgag
     4501 ttcaactgta tctaaaccca tatggatcag tatttttctg ccagattcac tttcgatcgt
     4561 tatggcatgt cgggtaggaa atatattatc gattattcct ttaactggcg catggatata
     4621 tccatcatga ttcgttatag cgtatccaac tcctatcatt tttgaagaaa agacctcgtc
     4681 ttttactgct gttaatggaa taatttgacc actagctaca gcttttagaa tgtttcttgt
     4741 tttaaagaaa tgcacttttt atcacctcta ctttaatgct ggccaatatt ttttgtttac
     4801 tttgatcatt tcatctagta ttttttttgc gactctggca tttggtacgg tttgattcaa
     4861 tgtaaatgct tgtaaagctt tttgataaga accttcaaag taagcatcta ctagtaactt
     4921 ctctactgca acttgtgcct ccatcaatcc tttatgaaaa tcactgattg gttttcttag
     4981 tgtaattggc tccacaccag ttgcaccaac atatgccggt atttcaacaa ctgcatctga
     5041 ccgtagattc ggaatcgccc cttgattagg aacgattagc ataaagcgac gacgatcatc
     5101 attcaacaag gaaatagcta tatcgacgat atattgtcca tgctccccaa aattaaagtt
     5161 caatacttct tccacaatac cgcggcgtat tttatcagcc atttctttgg tatttttctc
     5221 tcgtccatcc atgaccatat tagcacgtgt atactctttg tcagtatgct caacgaccat
     5281 atcaggatat aagtaatact ctaagtaatt attcgggata ttacttggaa aatttttcgt
     5341 gatgactgac atcatttgaa aggttttttg ccaagtttta tctcctatat taaaatcggc
     5401 gacttgcatt tcttttgtta ttagtttttc aataatctct ggcataattt ctcgttgcaa
     5461 ttctttatca taaatcgatg tataccagcc aaagtggttt aaaccataat acgtaacgat
     5521 ccaattctta cgatcataac cgtaattcac tgcaatcgtt tcttcaattg aaatagtcat
     5581 atcacaggca ttaatcattt ttacattcgg atatttacga cgaacagctt cagaaacgat
     5641 cgactctgga tttgtataat tcaaaatcca tgctttgggg gcatagtctt gtatatgatc
     5701 gacaatttcc aataaacctt tgattgagcg catcccataa gcaaatcctc cgagtccaca
     5761 cgtttcttgt cctactaatc catgtttaag tggaatcttt tcgtcctttt ctcgcatctc
     5821 caatcctcca acacgaattt gtgagaaaac aaaatcgcat cctgtaaaag caagctttgg
     5881 atcatccgtt gcacgaatgc gaatatttga aaaacctttt cttttcagca taaatttaac
     5941 aatcaagtac atatccatat ttctttcttc atcaatatca tataaacgta tttcagataa
     6001 tggcaaccgt ttttcattgt tcaaaatggc ttgaacgatt cctggtgtat acgtactccc
     6061 gcctccagct atagtgataa tctgtttttt catcatcatc ttttcctttc tattcttcta
     6121 ataatttatt gaattctgcc ataacataag tcacttctaa gccgatgata atttgataat
     6181 ttttttcagt tggtttaatg atcccgctta tccctaattg cttgattcgc tgttcctcga
     6241 ccatggtggg atccactacc tctacccgta ggcgtgtatt acaactatag acatccagga
     6301 tattggcttt tcctccaaac gcttgtagca tttttgttgc catatattca taatttttac
     6361 ttgctaaccc taactctgtt tcttctaagg ttacttcatt gccaaactct gccttagctt
     6421 cacgtcctaa tgtctgaatg tttctcttat tgatcaaata ataaaaagta acgtaataca
     6481 aggcaaaaaa gaaaattccg attggaagaa tcaaaatagc attggtagcg attcgaaaat
     6541 tgatgagata atcaacaata catgctccga atgtaaaacc aattcgtata ttgaataagt
     6601 aacagacaat ccctgctaaa ccggcaaaaa gtgcatggat cacgtacaac ataggagaaa
     6661 gaaacatgaa actaaattca acaggctcag tgatatttgc aataaatgaa gttcctgcac
     6721 cgcttcccat gattcctttg accatctctt tattttcttt ataagctgct ttatagatag
     6781 ctagacatat agctggtata ccaaacatca ttactacaaa aaaaactgat aaaaacagtc
     6841 cagcctgagg atctccgttg ataaagcgtg tcatctctcc tctgaaaact tccttgctag
     6901 gagatgtata gcttcctaaa tcgtaataaa tatacgtatt caacacatgg tgcagtccaa
     6961 aggggatcag caaacgattc aaaaacataa atatccccac acctaatgct cccatatcaa
     7021 cgagcagttt tgcaaaatta tcaatcccaa tttggatgag cggccagata aagcccaaaa
     7081 aaacagctgc gattgtttgt agaatcatca ccatagtcaa tgggaatttt tcgcccgcaa
     7141 aataagaaaa aactactggt aatttttgtt ctttaaagcg attatacgtc catgccgcta
     7201 ctaaaccgga aataattcca ccaaaaatcc ccatattcac ttctgggtcc ataatttcta
     7261 agcctttttt caaggtagca atcgctaaaa aaccagtaag tgcaggtatt cctttgtcct
     7321 tatttttgac aaaaccgatt gcaactccta tagcaaataa gatatccaag tttccaaaaa
     7381 cggcatttcc agctgcacca attactggga tgttcagcat atcgtcagct gataaacgca
     7441 tgatcaatcc cgctaatggc atcgctgcaa ctggaagtag catcgctctt ccaagcttct
     7501 gcacatttga tttaacattc atttttattc ctcctctatt aaatatcctc atatgttact
     7561 aactcaatca aaggatcatt tttcactttt tgagcaaatg ctgaatgaat taaaaattcg
     7621 acttggtggg tacgaggata cacaaaactt gaaccattta acaattcctg atccaaatac
     7681 gctgcgtgac acatgacttc gatcgtttta tcttctctca atatcatgag taaacttgcc
     7741 aaatattctt ccacactcgt attgatttgt cgtttttttt catcagtttc tcctacgaca
     7801 tcgaaacaag gctcaaaata atcgacatga cgaacttcat cttttcgttc aaagttgctc
     7861 cgaaccggca aatcatattt ttttgctaat gtaacaacta cctcctgatt ttcatccaaa
     7921 gtatgagtat gatgatgact gtctaaatga gttggtatga tccccgcatg ataaattttt
     7981 tgaatctgag cattccattc ctgatacagt tgatctttgt caatgttaaa tggatgctga
     8041 taataagata aggatctgaa ctctccgctt tcgataagag tatcgacagt tttgaggagt
     8101 ggtttgccac acgtcaatgt taaatggact cctacaccta gcctaggcat ttcctttctt
     8161 agtttcacag catgctcgaa acccggcata tttgccatca atgtagttga tgtcaaaatt
     8221 cctcgctgat aagcatccat aatcccataa ttcacaccat ggctatagcc aaaatcatcc
     8281 gaattaaaaa tcacttttcc catttcattc tccttctttt ttctactaaa ataggcgcaa
     8341 ccaaataatc atagtccatt tactatgtgt catttggttg cgcctataga tagttacgcc
     8401 cttattttaa tagattcttg ctagatgaat tgccaaatag atgatttcat tttcttgaat
     8461 ggcaagttga aattgctgtt cgatgatatc tttaatcttt agtgctgtac aatacgcttt
     8521 gctatgttta aatgtttcag taaaaaaacc aatattttct actggcgttt cttcctgata
     8581 aatcaagcgt ttaaccagga atttgatatg aaccattaag cgatgataag tcagtgattc
     8641 tttatcataa acgtttgaat aggatttttc tagatgggac aaaatcgtgg tcgtcatttc
     8701 tactattttt aagctcttta aactattagg ctctcctagc tcagcattaa tgatatgcat
     8761 agtgatgaat cctacttcat ttgaccctaa ctcggtttga tatcgatgat tgattcgctc
     8821 gaccacttct actgctatat catattcatt tggataaagg aatctcattt ctggcaacat
     8881 aattgctgtg acatctctat cttctctttt tcgttgaata gcaaaataaa tatgatctgt
     8941 caatgtaata tagatatttg aatctaatgg agaggagatc ctagctgtta tcagttcaat
     9001 cacttcttcc gcaaaatcca taaattcgat tggaatctgc gagactatct cactgaatcg
     9061 tttgatcttt tctttagaat ctaatacaaa taatttgtca gcatcctcaa catcaaatgg
     9121 cttactcact ttagcagaaa agccaatccc tttccccatc gcaatttttt ctattccatc
     9181 gtcatcgatc aatacagcat tatgattcag ctttttcatc acatacaccg ttaattcacc
     9241 tcctctatat gataaaacaa aacaacttgc agactccttc atacatattt ttgtctccaa
     9301 acaattttta aagacaaaaa acgacctaag aaagaaccct ttcttaggtc gtgcctaatt
     9361 gaatagtagc accctacata atttacaaaa atagtttaac acataataaa agcgttatca
     9421 atatcttttt tactcgtttt atgataatac ttgtatcgaa ttgatttgga ggtaattaca
     9481 taaaaacaaa aaaaa
//
LOCUS       DACDTO010000099         9352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDTO010000099 DACDTO010000000
VERSION     DACDTO010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.099"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(93..761)
                     /locus_tag="IWK70_002466"
     CDS             complement(93..761)
                     /locus_tag="IWK70_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9614966.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            895..1182
                     /locus_tag="IWK70_002467"
     CDS             895..1182
                     /locus_tag="IWK70_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614967.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1598..3136
                     /locus_tag="IWK70_002468"
     CDS             1598..3136
                     /locus_tag="IWK70_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9614968.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3133..4098
                     /locus_tag="IWK70_002469"
     CDS             3133..4098
                     /locus_tag="IWK70_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9614969.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4085..5407
                     /locus_tag="IWK70_002470"
     CDS             4085..5407
                     /locus_tag="IWK70_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9614970.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5470..5574
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5648..6391
                     /locus_tag="IWK70_002471"
     CDS             5648..6391
                     /locus_tag="IWK70_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9614971.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6406..7398
                     /locus_tag="IWK70_002472"
     CDS             6406..7398
                     /locus_tag="IWK70_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9614972.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7410..8147
                     /locus_tag="IWK70_002473"
     CDS             7410..8147
                     /locus_tag="IWK70_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9614973.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8137..8859
                     /locus_tag="IWK70_002474"
     CDS             8137..8859
                     /locus_tag="IWK70_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9614974.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8943..>9352)
                     /locus_tag="IWK70_002475"
     CDS             complement(8943..>9352)
                     /locus_tag="IWK70_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9614975.1"
                     /translation="QLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEKQAAP
                     VAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYLNGDY
                     SAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3067 a         1605 c         1969 g         2711 t
ORIGIN      
        1 atttttttat aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc
       61 tgtaaattaa ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca
      121 agtggttcaa atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt
      181 tccagatact tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac
      241 agaaaatgtg tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag
      301 aaaagacttt tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct
      361 gttccgcctg tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata
      421 cctgtcatca aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc
      481 cataaatgta aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt
      541 tttccaaaaa gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca
      601 gaaggagcga atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct
      661 gccagcttag atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa
      721 atgattccgc acaattctgt aaataaattt aattttttca tttctagcct cttttctttt
      781 gaaaattttc agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg
      841 aaaaaaaatt atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga
      901 gcagcagact tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt
      961 tttttggctc aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat
     1021 caatttatct ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt
     1081 tttttgatga agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct
     1141 ctttatggag tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa
     1201 gacgtttatg atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac
     1261 agtataatat tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta
     1321 ttggctgttt ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc
     1381 aagtgacaat ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag
     1441 ttgatccgac tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc
     1501 ttttttttgt tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa
     1561 atgtttatac ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt
     1621 aagtgaaaga aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat
     1681 ttctttgctg attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa
     1741 aaaaatggca gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc
     1801 cttattaggt ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact
     1861 tgttatttac gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga
     1921 cccgtctatt gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa
     1981 agtagagctt cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt
     2041 aatcatcggt acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat
     2101 tttgttaggt attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc
     2161 cttgcttgca attatattca gcggactgat tcgctggttg cagcataaga aacctcgata
     2221 ttccatcatt actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc
     2281 tgccttgttt aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat
     2341 cctgatccaa atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa
     2401 accgaatttt ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat
     2461 ctatcctgag ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa
     2521 aagtgctgat ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca
     2581 attcgatatg acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa
     2641 gagatctttt gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga
     2701 tcagatcaaa gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat
     2761 ccaagaagca tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta
     2821 ccaagcaatc aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct
     2881 caaggagtac gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg
     2941 cgcaccgctg atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa
     3001 taaattaagc ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt
     3061 cgaaaagaaa aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa
     3121 ggaggaagat cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga
     3181 aagtatgtca tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt
     3241 ggtccttccg gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca
     3301 tcagcaggag acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg
     3361 cggtggaata tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag
     3421 caaaatattg aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt
     3481 gtagatgaac ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct
     3541 agagagttgt ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc
     3601 ccagatgtga ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca
     3661 ttacaagaat tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg
     3721 cacaatatgg aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa
     3781 attatccaat gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg
     3841 catttctttg atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat
     3901 ggatatttct taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct
     3961 atgaaagaag tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa
     4021 agaagtattg ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa
     4081 ggaaatgaaa acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc
     4141 ttatggattg aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg
     4201 gacttgtcct aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg
     4261 taatcgggta aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga
     4321 agaacttcaa aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca
     4381 attagcagaa gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc
     4441 tattgaagtc aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag
     4501 acctacgatc ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc
     4561 attgcctgta ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct
     4621 agctacgatt gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc
     4681 attaaaagaa tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg
     4741 cattcagttt gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg
     4801 tttagtcgga aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca
     4861 gccgaataca gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat
     4921 tgcagtcaat gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt
     4981 ttcaaggaaa accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa
     5041 gcggataacg gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta
     5101 cgccagtcct aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca
     5161 agtggtagag atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc
     5221 taaggctgaa ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga
     5281 gcaagcagac gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa
     5341 agagttggat agctatatca tggggtatcc gacattggca agtgatctct cctatttatt
     5401 gaaataatca gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta
     5461 aaaaatgaat acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta
     5521 gacccattga acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc
     5581 gggttactat gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg
     5641 taacaaaatg aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg
     5701 gcagattgtt gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt
     5761 attttctgca ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa
     5821 tgaagcagtg cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat
     5881 ggattattgg aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt
     5941 gccaatcatg gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa
     6001 agtgatcctt gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt
     6061 gaggaaagta tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa
     6121 gatttatcga atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt
     6181 ggctgctact tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg
     6241 cttagggtat tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc
     6301 gatcatttgt gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa
     6361 agggtattat cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg
     6421 cttttattac cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc
     6481 aaacaagaaa taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact
     6541 aatcatactg gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat
     6601 gtccaaatca tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa
     6661 gcacaatttg gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat
     6721 ataccagtca aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta
     6781 tcagacagta acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa
     6841 agtccaagtg aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca
     6901 aaactgacaa tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat
     6961 atccaatggt actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta
     7021 aactatatcc cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt
     7081 acgaatgatc aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg
     7141 aaaaaaggct atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa
     7201 tatgcaaaag aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat
     7261 tatacggatg atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca
     7321 gcttttatgc aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact
     7381 aatcagttta taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag
     7441 tcattgaaaa cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta
     7501 catcgattgt cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag
     7561 tccagcctat gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata
     7621 cagcatttag ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa
     7681 acgttacgtt atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt
     7741 atctggatat ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg
     7801 ggatgaagca gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc
     7861 ttgatgaacc atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt
     7921 tgaagctgcc taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag
     7981 caatttattt atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg
     8041 agattcaagt gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga
     8101 agcaaacgat ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat
     8161 ttagatggga gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc
     8221 aattgtgatt cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa
     8281 gccttaagtt acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag
     8341 ccttttttga aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat
     8401 gtaccgatga ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat
     8461 gagaaacctg ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa
     8521 gcttacccta atcactattt gatccattgg tattcttctt tggactatca acaagactat
     8581 attgatttag gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg
     8641 caactagcta aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca
     8701 gcagagtggg ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa
     8761 cagttaaaga caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact
     8821 tatcaaaact tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta
     8881 gtgtcgcacc tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa
     8941 tcttagtacc aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt
     9001 gaagtaacat attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag
     9061 taagaagaat ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg
     9121 cttgattctt tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca
     9181 gtttctgtta cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt
     9241 tgaaccgttt cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct
     9301 tgaggttcag cagctgcttg tgcagagcct tctgttggga ttgttaattg tt
//
LOCUS       DACDTO010000100         9124 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDTO010000100 DACDTO010000000
VERSION     DACDTO010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.100"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(522..878)
                     /locus_tag="IWK70_002476"
     CDS             complement(522..878)
                     /locus_tag="IWK70_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP9614976.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(892..1302)
                     /locus_tag="IWK70_002477"
     CDS             complement(892..1302)
                     /locus_tag="IWK70_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9614977.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1649..3835)
                     /locus_tag="IWK70_002478"
     CDS             complement(1649..3835)
                     /locus_tag="IWK70_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9614978.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4053..4736
                     /locus_tag="IWK70_002479"
     CDS             4053..4736
                     /locus_tag="IWK70_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9614979.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4817..5536)
                     /locus_tag="IWK70_002480"
     CDS             complement(4817..5536)
                     /locus_tag="IWK70_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9614980.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(5848..6054)
                     /locus_tag="IWK70_002481"
     CDS             complement(5848..6054)
                     /locus_tag="IWK70_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614981.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6299..6901
                     /locus_tag="IWK70_002482"
     CDS             6299..6901
                     /locus_tag="IWK70_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9614982.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7042..8322
                     /locus_tag="IWK70_002483"
     CDS             7042..8322
                     /locus_tag="IWK70_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9614983.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8335..9063
                     /locus_tag="IWK70_002484"
     CDS             8335..9063
                     /locus_tag="IWK70_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9614984.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
BASE COUNT         2640 a         1703 c         1497 g         3284 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
      241 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      301 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      361 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      421 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      481 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      541 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      601 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      661 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      721 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      781 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      841 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      901 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      961 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
     1021 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
     1081 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
     1141 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1201 ggcaaaaata ttcttctctt caaattcaag ttgattcgct tgcaaccatg ctcttgcttt
     1261 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1321 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1381 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1441 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct
     1501 tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc
     1561 tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact
     1621 gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt
     1681 ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat
     1741 acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg
     1801 tcgtcactgg ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt
     1861 tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt
     1921 tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca
     1981 agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc
     2041 tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt
     2101 ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct
     2161 ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat
     2221 tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt
     2281 attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct
     2341 ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat
     2401 agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta
     2461 caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc
     2521 ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt
     2581 tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg
     2641 cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca
     2701 atctatttgc tccttggaca atagctgccc gagaacgata attagtcaat aagaaaagag
     2761 ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc
     2821 catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca
     2881 tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat
     2941 aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat
     3001 tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg
     3061 tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat
     3121 atttttcttt aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt
     3181 ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac
     3241 gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt
     3301 gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa
     3361 agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc
     3421 gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt
     3481 ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc
     3541 tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt
     3601 gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc
     3661 cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg
     3721 cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact
     3781 tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat
     3841 tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt
     3901 tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat
     3961 atgattttag ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa
     4021 gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta
     4081 caataggact tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat
     4141 atggtgcttt tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg
     4201 acggagaatg gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga
     4261 ttcttctaac aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct
     4321 tgtttgttct atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg
     4381 agcatcccgc attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt
     4441 ttgataaaat caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg
     4501 tagcaacgaa cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt
     4561 tccactatct aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc
     4621 atcatctgtt tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc
     4681 tcactcagac atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc
     4741 aatgaatcaa aaaaactaag caagatgctg ctcacagaga aatatctgcg agtgagcaat
     4801 ttgcttagtt tttatattat ttatttgtaa agattccctt tgctttcggg ttatttgtag
     4861 tgtagcggat cgactgggca tctgcctctt ctgtcatttt ctttgcccgc atcttgattt
     4921 cttgtacgat ttgttctcca catgccggat cggctacgaa atcatatttg tccccatcga
     4981 tcactatttt gggacatata tcaaactgtt cataccactc ttcgtatcgt cgattgagtt
     5041 ctttatagta ggaatagagt tcaggatctt gttctagctg ttcatattcc cggcctcttt
     5101 ttttgattct cgctaacata gtttcaaagg atacacgaat ataaaccagt agatctggtt
     5161 tcttttgtgg cgaaatttct tctaactcct tcagcatcgt ttctaaaaga ccttcatact
     5221 gctttacttc tacttcagaa acacgcccta aatctgcatt caaatgaaac agcatactat
     5281 cttcgtagat ggagcggtct agtacgttgt catcatggct gaaagcatct ttgattgcca
     5341 aaaagcgttt gttcaaaaag aaaatctgta ataaaaaagt atacttttct ggattagaat
     5401 aaaatagcgg caatacttca ttatcttcca cgttctcata aaaaggtttt gtttctaaat
     5461 cttgggcaag catttcagtt agtgtgcttt tccctgcacc gatcgttcct gcaacaatga
     5521 tcacaattat cggcatcctt cctgtaataa aatctgttct tcattttact acttcattta
     5581 tagaaataaa agccttgact ttatctgctc cttaaaagct acttctctaa aagtagccaa
     5641 aagtaactcc tattcatgaa aataaaggct gtgacaaaag tcttgtcgca gccctttttt
     5701 caaataaaca gtattctcct gccaacacct cggtcacaag gcaatattca taaaacatga
     5761 aaagctgttt tttaacatta ttccttgtaa ctcagagcta aacgggagac tcacaacctc
     5821 tttatgctct caacatttta tctgttttta ttcttctatt gtacttgatt tgcgtacaac
     5881 taactgccag tgcagattat gagcggtatc attgtcttct actgcgcatt tcatgacacg
     5941 tatcatcaca ctattttcat agattttttc tatttttcct tttattggtg attcaaataa
     6001 atcagattta aatgcatatg tttcgccgac ttcaaacaat tgttcatttt ccataagaat
     6061 ccctccatca ccttttataa ggtcagtaat tttttaacat aaatcactcg aaaaagcaca
     6121 aacttgtcat aaaaagtact tttcgtatca aaactgtcac tttttttggt aaggttcttg
     6181 gaaaacgaag atttttttag gagatcatga cggttttgat gtcaaaacca ctttttatga
     6241 aacaaaagac gttttttatt ttttttagca taaaatttgt ggtggaggta cgtatgagat
     6301 gaaagataaa gttgtgaaca aattattaga agccttattc agcggagaca aatcggaaga
     6361 agatatggtg tatgtccaag taaaatttgc cttagaagtt ttattgaata atcttggaaa
     6421 actagcggtc gttctagtct tttctctggt gacaggctca tgggcggaaa caggaatcac
     6481 ctttctttct tatatctgca tccgcagata tgcttatgga ttgcattccg actctgaatt
     6541 cgtttgtctg ctgtggacac tactttattt atggggcgtt cccttggtga tgaaacatct
     6601 gcaattgact atctccttcc caatgatggt ctttttgtta atcagctgct ttttgttact
     6661 gcttcgctat ggatcaagag gtacagcaat caatcccatt gaacccgaga aacgtccccc
     6721 attattaaag aaggcgatca gcatgtttct aattttttct ctcattactt tatttttctc
     6781 agcttcttac ttttctacct atttgctact tgggattgtt ttagaaatag ctacgttgct
     6841 tcctattaca aactacttga tgaattttgg aggaatcttt catgaaaaaa acaatcatta
     6901 aacaattaac taacttatct gacttttttg tatctggctg ctgctttggt atgagttctg
     6961 aattcaaaat ccctgaaaaa ttaaaaaaat aatacgctaa ttgtgctata atttggtaaa
     7021 aatcaatggg agctgtactt tatgttttta tggtccatag cgttgcaata tttgatttac
     7081 acaatttgta tcttattgat tgaccatcac atccgataca aatatctgtt tgcgggagtc
     7141 attctgattt cttgtatttt gattggatgg gtctattcga ttttagggat ttttactgct
     7201 ctaattgcta taggaatgct gatgcttact tttttcttct tttcgacttc gcgtcaacaa
     7261 ttggtttacg cgcttccttt tgggttattg acgtcgcttt taggagatca cttaacaagc
     7321 atcgacgatt tttttgtttt gaaatcttcg gttattgaac caggagattc attgcagtat
     7381 tttcacattg tcttatccgc ttttttatct gctttattag ctttttgttt aaaaaaatcg
     7441 ttagagactt ggtttcacac tgtggaccgc cgtatgatcg gtacgatcgg gacattgata
     7501 ttatttacat actatatcat tattttttat acccgttttt ctggtgaaac acctgatgtc
     7561 ttagctttga atacgagttt tttcattcta tatctgtgtg ttggcctgat catcttggtt
     7621 tattattgga aaatctctaa taagaaatta gctgatcagt tattagagca acaagtcagg
     7681 atgcaacagg attatatcaa agatctagaa aaaaactatc aagacttgag agaattcaaa
     7741 catgattacc aaaatctgct tttttctctt cacagctata tttcagaagg cgacttagaa
     7801 ggattacggt cttattatga tcaaacgata ttacctacac gccagatgat gaaccttttt
     7861 ccggccaatc taagtatgtt ggataatatg aaagttcctg agatccgtag tttactgtca
     7921 ttaaaactca tgatggcaca ggaaaaaggg ttgatcgtcc agcttcattt tcctgagcca
     7981 atcgaactgg atacgaaaca tacggtaaac cttgtacgta tgcttggtat tatcttggac
     8041 aatgcgattg aaggagctag tgctgcaaag aagaaaaaaa tcgaattgac catactcaag
     8101 aagaatcatt ccgtgatgat tcgtgtgatc aatacaacaa caaatacatt accgttaaac
     8161 cagttgaaac aaaaagggtt ctcaacaaaa acaaatccaa aaaacgaagg ccttggcttg
     8221 tttatcttgg atgaattgac aaagaccaat ccttatcttt tccttgaaac atcaattgaa
     8281 aaccagcgtt tttctcaaac gatttatata caaataaaat aaaggagtaa tgatatgatt
     8341 cccatttata tttgtgaaga cgatccaaaa caattagaag ttttatcaac ggtgatcaaa
     8401 aatagaatca tgattgaaaa cttagacagt tatgtccata tttctacctc tgatccggaa
     8461 gaacttctca atgcagccaa gatcagaact tcttcttttg gtatttactt tttagatatc
     8521 gaactagaag acagtgaaat ggatggtgta gcgatcggta aattgatccg tgaattagat
     8581 cctttaggaa aaataatcta tgtgacttcc catacggaaa tgtcgatgaa aatcttaaaa
     8641 agcaacatcg aaccgactga ttatatcgtc aaagaagatc tttatgattt aaaagaaaat
     8701 gtcgagcata tcctgagcaa aattttcgaa gattttcaaa tgtcccaaat ccaaaaagat
     8761 atcttcaaaa tagagtttaa tgatgagatc aaatttttac cgatcaaaga catccaatat
     8821 ttttctactt cgctaggagc gccacataaa ctggaagtcc atttgactca tgcccaatta
     8881 caattctatg agaagatcaa agaaatcgag aaaatgcatc ggaattttat ccgttgtcat
     8941 aaatcctatg ttatcaatac acaaaatgtg cggtcgatta ataaaaaaac cagagaagtc
     9001 acgttggcaa acggcgagac catcccagcc tctatccgtg gattaaagaa attgatttca
     9061 taaaaagaac cgaatagttt ggaccgacta aatgaccagc cgatccaaac ccttcggttc
     9121 tttt
//
LOCUS       DACDTO010000101         9046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDTO010000101 DACDTO010000000
VERSION     DACDTO010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.101"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWK70_002485"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWK70_002485"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9614985.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            2714..2938
                     /locus_tag="IWK70_002486"
     CDS             2714..2938
                     /locus_tag="IWK70_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9614986.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            2951..3451
                     /locus_tag="IWK70_002487"
     CDS             2951..3451
                     /locus_tag="IWK70_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9614987.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            3548..5395
                     /locus_tag="IWK70_002488"
     CDS             3548..5395
                     /locus_tag="IWK70_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9614988.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            5398..6294
                     /locus_tag="IWK70_002489"
     CDS             5398..6294
                     /locus_tag="IWK70_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9614989.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            6344..6733
                     /locus_tag="IWK70_002490"
     CDS             6344..6733
                     /locus_tag="IWK70_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9614990.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            6720..>9046
                     /locus_tag="IWK70_002491"
     CDS             6720..>9046
                     /locus_tag="IWK70_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9614991.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLE"
BASE COUNT         3196 a         1361 c         1908 g         2581 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatatat caatggtttt taaccaatgc
     2761 tcaatcttta gtcttactag cgattgtggt cattggtttg tttttaggat ttaaacgcga
     2821 gttttccaaa ctgattggct tcttggtgat tgctttgatt gcggtaggtc tggtctttaa
     2881 tgcttctggt gtgaaggatg tcttactcaa tttgtttaac cgaattgtcg gtgcctgagg
     2941 gggaatcaca atggaacaga tacgtgttta tattgcgaac cttggaaaat acaatgaagg
     3001 tgagttggtg ggggcatggt ttaccccacc cgtcgatttt gatgaagtga aagaacggat
     3061 tggattaaat gacgaatatg aagagtacgc aattcatgat tatgaactgc cttttgagat
     3121 tgatgagtat acaccaattg aggaaattaa tcggttgtgt ggtttagcgg aagagcttaa
     3181 aggaacgcca attggtgagg tcgcttcaga gattcaacat gccttcttca attctttcga
     3241 ggaaatggta gagcatgtgg atgatatcat ctattatccc gattgtaatg acatgagtga
     3301 tgtagcgtat tacttgattg tagaagctgg tgatttaggc gaagtaccag aacacttgaa
     3361 gacgtatatt gattttgatg cgtgtggtcg agacttggag attggcggga attatttggt
     3421 tacgagtcga ggggtttttg aactaatgta aatcatttgt tagaattgac cagaaatttc
     3481 tatcgtactt ttacttttct atttgatata ctgaaataga aaatatttgt tgataggggt
     3541 gttactaatg gttggcaata agtggtacaa atgtgatctt catattcatt caactgcgag
     3601 tcagtgcttt caggataaaa aggttactgc taaggaatgg gtagaagaat gtttaaacaa
     3661 aggactcgat tgtgttgcat taactgatca caatacaggg gaaaatattg atgaatataa
     3721 gaaagtggct atcgaaaatg gcttagattt ttttcctgga gtggaattaa cgtgtggaga
     3781 aaatggaact catttactag tcctatttga taaaagtgca tgtacaaatg atatctctga
     3841 tttcttaacc aagatgggta taaaaagaga ggattgggcc agcgctgcag cttgctcaat
     3901 gaaatcagtt ttagaagtta ttgattttgc tgagcacgag ggcttgctcg ttattcctgc
     3961 acatatagat gagtttaatg gaatttcttc agcaaaattt ggaaatctag gaaagatatt
     4021 tgaatcagaa aatatatatg cagtacagac ggttcaaaaa gaattttttg aaaatcgctc
     4081 acaaatgatt ccctcatcta agcgtgacac tatctatgat agtgtgaatg ccagatatga
     4141 tggtagagtt ggaaaagata ctttggaatc ttggtataaa tcagttgttg aggcagaaaa
     4201 aaataatatg acatttctta gcttttctga taacccacac tcgaaaggaa attcaaaaca
     4261 tggactttgg ggaataggca caagatattc ctatataaaa atgaaggacg aacctgactt
     4321 gggatcactt cgcgatgctt taatgctagg cgaaactcga gttcattctg atttttctaa
     4381 tttctccatt aatgaaaatg aagttttatt ggaaaaatta agcttttctg ggaccactct
     4441 tagcacaaaa gaagttgtag tcgaattcag cgataattta acttcaatta ttggaggaag
     4501 aggaacaggc aaatcttgta ttacaagatt cttggtctat gtccttggaa aggaagcaga
     4561 acttgatcaa ttttctgaaa tacaaagtga ttatcagaac tttgcacaaa ttgaacataa
     4621 tggctctgga atatttttga aggatacaat tgtgaaactg aatattttct ataaggggac
     4681 aaaatatcaa ataataagaa cacaggatag acattctatt tatgaattta cacaagagaa
     4741 tggtctggtt gaaaaagaaa cagaacggtt gagaatgatt tccgataagg tgagtatata
     4801 ctctcaaaaa caaatttatg aaatttcaaa aaatcaaact agtatactag aattactaga
     4861 tggatataac tctgatttga tttcagagta taaaaatgag attgaaacat gtgtaaacga
     4921 aataagaaaa ttaaattggg atattgtttc tgtgaaaaaa gaaatacaag ataaggcgaa
     4981 ggtagagctt gaaattgaag atttaaaaaa acaagtagaa aagctatctc ataagagtta
     5041 taaagatgtt tatgatgagt attcaaaaga gacagatatt tatagggaac tgaaacgtga
     5101 tgtggaggca cttaaggaaa ttcccaaaaa tctaactgat agtgttgaca aaattgactt
     5161 tggcaatggt ataaaagtta cagacgaaat tgatgaacac cgcggagaat tgattaagaa
     5221 tttagtgacc aatcgggcta agattcaaac aataattaat gaaatgtcag atgaaattga
     5281 tagttataga gcagagctga ataaatcaga ttggaaagtt aactataatg aggtttcaaa
     5341 gaggtatcaa agaggtatca aaaacttagt gaagagttat ctcaagatga attaaaaatg
     5401 ttacaaaacc ttgattcttt atatcaatct ttatcaaaaa ctgaagagcg attggaagaa
     5461 attattcaac aagaaataaa gcttaaagat taccaagttg aggttgactc agcaatgtca
     5521 agaatgaata aatcatatca aaatctctat tctgagagaa gtagttcctc gcgcgaaatt
     5581 ttttctgata ttacaggaat agaggctact attcaaccaa atagggattt caataattat
     5641 gttaaagagc tcagagcttg tattaataag gaggacactt ttgacagtga gtttggatct
     5701 cttattgata agctgacaaa aaattccatt actaatcaag atatatataa tgacatactt
     5761 gatataagag ttagtgggga aagtagtatt ttttcagata aaaaattaat caattctata
     5821 agaaaattga atgatgcaca agttcttgaa ttacaaaccc ttaagccaaa tgatttagta
     5881 gttatccgac ttgaactaaa tggaaaaaaa gttagtttat cgaatgctag tgctggtcaa
     5941 aagacatctg caattctttc tatgattttg gcgtatggaa attcaccact catattggat
     6001 cagcctgaag atgatttgga tagtcaatta attaataact taatcgtaag aagtatcatt
     6061 acaaaaaagg aaaacagaca aattttaatt gtaactcata atgcaaatat cccagttaat
     6121 ggtgattcag aatggttgat tagcatggga gattcaaaag aaatatcggc tgatcagtgc
     6181 gggtcggtag atgaacccaa tatcaagcca agaatatgta ctgtgatgga aggtggcgag
     6241 gatgctttta caaatagagc gaaacgctat ggttttaaac aaattgtaga ataacactag
     6301 agggtgagac atattattat gtccgccctt tttaggagga aaaatgaaaa aaataaaaag
     6361 ctataccagt atctgggcag ttgaaaaagt catttacgca atcaatgatt ttcagttgcc
     6421 ttttcctgtg acttttaacc aaatggcttg gttcgtttta tctttactgt ttgtcatcgt
     6481 atttgcccat gtgcctccac tatctatgat agaggaggtc tttttaaaat acttaggcat
     6541 accggtggct gtcacttggt ttatgtcgca aaagacgttt gatggcaaga aaccactggg
     6601 atttttacga tcctttatca gctatcactt gcgatttaag gtgacttttg caggaaagaa
     6661 agtcaaagaa cagaagaaac gatgggatga acccagtact cttgtaagga gtgtgaacta
     6721 tgtatccgat taagtatatt gaaaataatc tcgtctttaa ccaagaaggc gagtgttttg
     6781 cctactatga attagtgcct tacaattact cctttttgtc acccgaacag aaatttcaag
     6841 tgcatgataa ctttcgccaa ctaattgcgc aaaatcgtga aggcaagatc catgcgttac
     6901 aaattgccac agaaagtagt attcgcgcca cacaagaacg atcaaaaaaa gaaattaccg
     6961 gtagactcaa agaagtagcg aaacaacgaa ttgatataca gacagatgca ttagtatcca
     7021 tgattggcga tagtcagatt gattatcgct tttttattgg ctttaaacta attgcgacag
     7081 atgaagaagt caatctaaag agtctcaaga aatctttctt ttctgggctt caagaatttg
     7141 tctatggagt gaatcatcat ttgatgggcg attttgtttc cttatccaat gaagaaattc
     7201 gctgctattc taagcttgaa aaattaatgg aaagcaaatt ggctcgaaga tttaaagtca
     7261 ggcgagtcac accgagtgac ctgacgtatt tgattgaaca tatttatggc gaaaaaggga
     7321 taccgtttga agaatacgaa tttcagctac caaagaaaaa gctaaaatca gaaacactgg
     7381 tgaaacgtta tgacttatta cgtccaaatc gttgtttaat tgaagaaaaa ccaaggtgct
     7441 tacggatgga acatgagaat catgagtcgt acgtagcgta tttaacaatt aatacaattg
     7501 tgggggagat ggaatttcct tcctcagaac ttttctatta ccagcaacaa caatttacat
     7561 tcccaatcga cacaagtatg aatgtagaga ttgtgacgaa taaaaaggct ttagctaccg
     7621 ttcgtaataa gaaaaaagaa ctgaaagatt tagacaatca tgcttaccaa tctgacaatg
     7681 aaacgaattc caatgtctta gatgctttgg attccgtgga tgaattggaa accaccttgg
     7741 atcagtcaaa agaatctatg tacaaactga gttatgtggt tcgagtgagt gccgaatcag
     7801 tggatgaact aaagcgacgc tgtgatgaag tgctcgattt ttatgatgat accaatgtaa
     7861 aactagtccg gccctttggc gatatgatgg ggctacatga agaattctta ccctcaagta
     7921 aacgatatat gaacgactat attcagtatg ttacgtctga ttttttggcg gggcttggtt
     7981 ttggggctac tcagatgcta ggagaattag aagggattta ctttggctac aatgtggata
     8041 caggtcgtaa tgtttacctg aaaccggcat tggcttcaca aggagtcaag ggttcagtaa
     8101 ccaatgcgct ggccgccgct ttcctgggtt ctcttggtgg aggtaaatcc tttagtaaca
     8161 atctcttagt ctactatgct gttttgttcg gtggtcaagc tgtgattgtc gatccaaaag
     8221 gggagcgagg agggtggaaa gaaaccttgc cagaaattgc ggatgaaatc agtatcctca
     8281 acttgacgag taagtcagaa aatcaaggtc tgcttgatcc ttatgtgatt atgaagaaaa
     8341 agaaagactc ggaaagttta gcaattgata ttctgacttt tttaacagga atttcaagtc
     8401 gagacgggga gaagtttcct gtgttacgta aagccattcg aacggtgact caaagtgatc
     8461 aacgggggtt attacaagtt attaacgaat tgcgaaaaga cgggtcacca atcgcggaaa
     8521 atatcgccga tcatatcgag tcctttaccg attatgattt tgctcatttg cttttctcag
     8581 acggctcgat tacccaatcg attagtttag ataaacagct aaatatcatt caagtggcgg
     8641 atttggtttt accggatgca gaaacgactt ttgaagaata taccaccatg gaattgctta
     8701 gtgtttcgat gctaatcgtc atttccactt ttgccttgga ctttatccat agtaaccgag
     8761 aaatcttcaa aattgtcgat ttggatgagg cttggagttt tcttcaagtg gctcaaggaa
     8821 aaacgttatc gaataagctt gttcgtgctg gtcgggccat gaatgctggg gtctatttcg
     8881 tcactcaaaa tagcgacgat ttattagatg aacgtttaaa aaataatatc gggttgaagt
     8941 ttgcttttcg cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggtttgg
     9001 atcaggagga tcaaagcaat caaaaacgcc tgcgagattt ggaaaa
//
LOCUS       DACDTO010000102         8946 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDTO010000102 DACDTO010000000
VERSION     DACDTO010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.102"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..88)
                     /locus_tag="IWK70_002492"
                     /pseudo
     CDS             complement(1..88)
                     /locus_tag="IWK70_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(353..808)
                     /gene="tnpA"
                     /locus_tag="IWK70_002493"
     CDS             complement(353..808)
                     /gene="tnpA"
                     /locus_tag="IWK70_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9614992.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1051..2477
                     /locus_tag="IWK70_002494"
     CDS             join(1051..1624,1624..2477)
                     /locus_tag="IWK70_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa10 family transposase"
                     /protein_id="HAP9614993.1"
                     /translation="MAKYTFELKLKIVHDYLDGKGGSDYLAKKYSIKAPSQVKRWINA
                     YQEFGEEGLVRKRQKKKYSVQFKLDAIELYLTSELSYREVANTLNMNNPNLIASWMRQ
                     FREGGIDGLSKTKGCPPILSKKNEPKNKKKSTTKATSKERERIEELEKRVRSLQIENA
                     FLKELRKLRKQEAQQRRMKQSHESSQASEDRFKLVELLETLKFPKAIFMYWQKRLDRK
                     NPDQEIEAEMLKVREKHKDYGCLRMTNELRNRGFSINKKKVQRLIKKLGIKVTTYTRK
                     SRRYNSYRGQVGAVAKNRIHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLD
                     PFMDMFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGWAYQMKAYR
                     NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCFEELKSAIDSYIYYY
                     NNERIKQKLNWQSPVQFRKTTVTVV"
     gene            complement(2647..3609)
                     /locus_tag="IWK70_002495"
     CDS             complement(2647..3609)
                     /locus_tag="IWK70_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9614994.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(3611..5050)
                     /locus_tag="IWK70_002496"
     CDS             complement(3611..5050)
                     /locus_tag="IWK70_002496"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9614995.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            5235..7181
                     /locus_tag="IWK70_002497"
     CDS             5235..7181
                     /locus_tag="IWK70_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9614996.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            7445..8317
                     /locus_tag="IWK70_002498"
     CDS             7445..8317
                     /locus_tag="IWK70_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9614997.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            8613..>8946
                     /locus_tag="IWK70_002499"
     CDS             8613..>8946
                     /locus_tag="IWK70_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9614998.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALECFEQYKENKRRCGRTEIIFPAEEKEYIAKRSTEGWTPDVIIGRAERTFS
                     CSVSTLYRR"
BASE COUNT         2872 a         1601 c         1616 g         2857 t
ORIGIN      
        1 tagacagatc atcaatactt acgtaactca aaaatacttc caaaggtgtt cgataatcca
       61 gtgattttct aggaagatta tttctgcgcc agcattttct aatgtttgaa tttgtgtttt
      121 gtaatcttgg ttgcttgtgc taattcttga atagccgtaa attgccaaaa tttcatcacc
      181 ctttctggac ataagtttct gacaccagtt aaaatcaagt ctatcagtaa cccatacacc
      241 cgtgaggacg ggtgtatttt taatccccct agaaggggtc gacactcgct gccccttaaa
      301 aggggatttt taaaagaccg ccaactgctc atcttcactc acttacttct ctttattttt
      361 taaatggttc tacgtactct cttttactta taatatatcg aatgcgatca tcagcttctt
      421 gttcacggat gtatttttca tttgtctttt gattcaatcc gactgtactc acatagtatc
      481 tcttcgacca aaaggatttg tttccgtatt tatatttgag atttgcgtgc tgctcgtgga
      541 ttagtaccgc actacgacct ttcagatacc ccataaaact ggatatacat atttttggtg
      601 gtactcgaac aagcatgtga atatgatcta gcattgcatg tgcttctatg atttccacat
      661 ctttcatttc gcacaacttc ctaagtattt ttccaagatc ttttcgtaat tgcccataga
      721 ttacttttcg ttgatatttt ggtgtaaaaa taatatgata cttacaattc caacgagtat
      781 atgctaaact tttatcgtca tttgacatga aaaaactcct ttcgatctga tgagcggttg
      841 gtagccactc actagtatat cgaaaggagt ttttatcgct aaagatactt cttccacgcg
      901 tagaaacgcg cggttttctt gtttcgcacg ctttacgcac aaaactgact gaagtcatta
      961 agaaaataaa agggtctaaa acttttaagt tttgtaccga cccccaaaag ttagaccaaa
     1021 aaatctaact tttggaggtc ggtgtttttt atggctaagt atacttttga acttaaatta
     1081 aagatcgttc atgattactt ggatggtaaa ggcggaagtg attatctggc aaagaaatat
     1141 tccattaagg ctccatcaca agtgaagcgc tggatcaatg cctatcaaga atttggtgaa
     1201 gagggtctag taagaaagcg acaaaagaaa aaatattctg ttcaatttaa gctggatgcg
     1261 atagagttat atctaacaag tgagctgtcc tatcgtgaag ttgccaacac acttaacatg
     1321 aacaacccta atttgatcgc tagttggatg cgtcaattcc gtgagggtgg gatcgatgga
     1381 ctctcaaaaa cgaaaggatg tccacctatc ttgtctaaga aaaatgaacc caaaaataag
     1441 aaaaaatcta caactaaagc aacgtctaaa gaacgtgaac gcatcgagga gttggagaaa
     1501 cgagtccgtt cccttcaaat tgaaaatgcc tttttaaaag aattgaggaa gctgcgaaaa
     1561 caggaagccc aacaacgacg aatgaagcaa tcgcacgaat catcgcaagc ctccgaggac
     1621 cgtttaaatt agttgagctt ctagaaactt taaagttccc taaggcaatc tttatgtact
     1681 ggcaaaaacg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaag
     1741 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
     1801 gtttctctat caataaaaag aaagttcagc gcctgatcaa gaagctagga ataaaagtaa
     1861 cgacatacac acgtaaatca cggcgctaca attcttatcg aggacaagtc ggagcagtag
     1921 ccaaaaatcg gatccatcgt cgtttctaca ccagtatctg ccatcagaaa atcacgacgg
     1981 atacaactga atttaaatac catgaagtgg atgccaaagg cattatccgt caaaagaaat
     2041 tgtatctaga tccttttatg gatatgttca atagtgagat tttatcctat cgtatctctg
     2101 aaaagcccaa tgcattagcg gtaatggaag gtttggagga agctatccag accactaacg
     2161 attgtcccta tcgtcgtacg tttcactcag atcaaggttg ggcgtatcaa atgaaagctt
     2221 atagaaacaa attgaaggag tacaaaatct ttcaaagcat gtctcggaaa gggaactgct
     2281 tagataattc acttatggag aatttttttg gcttattaaa acaagagatt tttcacggga
     2341 aagtttataa ctgtttcgag gaattaaaat cagccatcga tagctatatc tattactaca
     2401 ataatgaacg gataaaacag aaattaaact ggcaaagtcc cgtccaattt cgtaaaacaa
     2461 ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa aaaggagtgg atttctccac
     2521 tccaaaaaag tctaactttt gggggtcact acaagttgac aactcctttt tgtttacaaa
     2581 tcaattggat atctacaaat tgtattttct aacgaatttt atagacatta aaaaattaat
     2641 ttaacgttaa atacttcctc cagaaagtat tgagattggt aaaatataat ccttatttgt
     2701 cttttcgcct ttaatttttt tcagaagaat atcaacaata agacttgcaa tttcatcaat
     2761 tggttgtctt attgttgtca attgtgggaa aaatttctca ataaaagagg taccatcgta
     2821 tccaataatt tttaaatctt ctgggatatt aagtttaagt tgatgagcaa ttttcatagt
     2881 gagaatggct gttaaatcat cagaaacaaa aataccatct ggtttgtgat ttataatagt
     2941 tgactttatt tccatttcac gacggatagt agacagatta ttaggaattt tgaaaatttt
     3001 tccatttgga ttttgaaaag aaaaacctag tgcacgtaat tctgtgggag aatccgtgtt
     3061 atcatttcct gtaatcataa tggtattttg acaaccattt ttttgtaaag tcttagctgc
     3121 aagttttccc ccttcaaagt tatctgaaga aacaatggaa acattaggag ctaaatttct
     3181 atcaaaagca acaataggtg ctctcacacg ctcataatca gcaattccaa gattatggct
     3241 tgatgagatt atgccatcaa cttggttggc ttcaagcatt tcaatgtatt ctctctcttt
     3301 agcaggatca ttttgactat tgcaaataat cgttttatat ccatgtttaa ataactctat
     3361 ttcgagatac tcaatgagtt cagagtaaaa gatattacta atattaggaa aaataagacc
     3421 aataagctga ggtgactttc cttgaagtcc acgtgctaaa ttatttggtt tataaccaag
     3481 agttttcata gcttcatgaa ctttttgttt tgttttttct gataaatatc ccttattatt
     3541 aatgacgcgt gaaacggttg ttgggctaac tccagctaat tcagcgacat cagttaattt
     3601 tgctaccatt ttaatatttt aattcaaagt attttccagt aggttcgcct gatttgattt
     3661 gaataccatt ttggtgattt tcagggaata cacgactagt aaacactttt tctcctttat
     3721 taataaaaat ctcaatgatt gagttatcta caaaaatgtt agcagttgtt tctttagtat
     3781 caattgagca ttcacgaatc gtaccaaagt cagtggcata ttgaatgcct gctttgctgc
     3841 gatctaaaat aatttttcct tctttggtat ctacaatcaa gctaagtcca tttcctttac
     3901 tatcagagaa aagaagtaac tccgatttct gatttggagg aaaagttagt tcaagctcat
     3961 aagtattatt tgtttctgct tttgctgtaa attgttcaga actagcacgt aatgatgtaa
     4021 tagcttctac tggatattgg taaagtttac catcttttat tgaaagttct tttacaagac
     4081 tcattgctcc ctgataatca tacttatcag ttggataatc gatatcaggt agtccaatcc
     4141 aactgacaat cagagcacga ccatcaggag tattgaatcc ttgagtcgca tacgcttcaa
     4201 atccataatc aagattttga atttctgaag cgcccacaag ttttactttt tctatgtcaa
     4261 gcgattgaca gatcttaaaa gtattcggat agatattccc ataatttagc tcagatttat
     4321 ccaacccttg aggacaataa atgagaacag gttttttgtc tacaaatact agatttggac
     4381 actcaatcat gtattctgaa ccagtgccgc caaaatcaag gttgccaact tcctcccatg
     4441 tttccacgtt attgtcatcc gctttgtaaa gtttgataaa gcctgatttt tctaaatttt
     4501 gtgctccaac aatcgcatac aattgtcctt cataattaaa catttgtgga tcacgaaaat
     4561 gatcagttac atcagcaggt ttatgaataa gaacattctc acctttcgta atgttgtagt
     4621 ctttatccat ccatgctccg atttgaagtg tatcacgttc ccaattttca tttcgaacgt
     4681 ttcctgtata gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc
     4741 cgtggctatc tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta
     4801 aatcatctga ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat
     4861 tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca
     4921 atcctgtttt aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct
     4981 taattttatc catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag
     5041 gtaagttcat attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc
     5101 cgttgaaaaa tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat
     5161 ttttaagata tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa
     5221 aaggagtgat tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa
     5281 aagataacct tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg
     5341 acacgaaagt gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa
     5401 ttaatggaca atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac
     5461 ttatcaaaaa aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta
     5521 aagataaaaa attcaacccc attatggcat taattaaatt attatcagat atttttgttc
     5581 caattatacc tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag
     5641 ctgctggact ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt
     5701 cctcaatgat ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta
     5761 tttcagctgc taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca
     5821 tgacaacacc agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt
     5881 cacaaacaaa ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta
     5941 tattagaaaa attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat
     6001 tattatcagt aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat
     6061 tgttgtctaa tggaattaca gacattattg tttggctata taacactact ggatttattg
     6121 gaatgggtat ttttggagga acatattcac ttatagtcat gactggttta catcaatcat
     6181 ttccagctat tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact
     6241 ttatctttgt agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt
     6301 ggttcttaac taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac
     6361 ttcttggaat tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct
     6421 tttgtgcttt aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg
     6481 ctgtttcact tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc
     6541 cattttacgt attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct
     6601 acggtaaaac aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag
     6661 ttaattcttc aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa
     6721 caaaagtttc atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt
     6781 cagtaaatga tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa
     6841 atggaaacac ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac
     6901 atgcttatgg tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata
     6961 ctgtatcaat gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa
     7021 aaggcgatat tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata
     7081 caacaatgat tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa
     7141 atacagtcac tgagggtact gatttacttg aaatcaaata aatatatttc cctgagtaaa
     7201 gatggaattt tctttccaaa atcaaataat atagattaat caaatatttg agaataacat
     7261 tttatttaat cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat
     7321 aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata
     7381 atttaaatat gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat
     7441 gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa tttgtgtgtg ccattggcga
     7501 tgaagaaatg acgatcaagg aaagagtgag ctttccgaca acgacacctg aggaaacaat
     7561 gcctttagtc attgacttct tcaaacaata ccaagcagat ttagcaggga ttgggattgg
     7621 ctcatttggg cctatcgata tccatcgcga ttccgccact tatgggtaca ttacctcgac
     7681 cccgaaattg gcatggcaaa attttgattt catcggtacc atgaaaaaag aatttccgat
     7741 cccgatttct tggacaacag atgtcaatgc ggctgcctat ggtgagtatg tcttcggcag
     7801 tggcaaagga ttgagcagtg tcgtttacta tacgatcggt acgggtgttg gcggcggtgc
     7861 cttacaagaa gggcgcttta tcgaagggtt tagtcatcca gaaatggggc atatgctcgt
     7921 cgtccctcat ccaaaagatg actttgctgg cagctgtcca tttcatggca actgtttaga
     7981 agggatggcc gctggacccg ccatcgaaaa acgcttaggg aaaaaaggac aagaggttgc
     8041 agaagatgat ccttattggg aaatcgaagc ctcctatatt gctcaatgtg cctacaatac
     8101 aaccttgatg ttttctccag atgtgatcat ttttggcggt ggtgtgatga aacaacgcca
     8161 tatgctgcaa aacgtccacg acgcatttgc aaaattggtc aatgggtatg tcgaaacacc
     8221 tgagttaagc aaatatatcg tgaccccagc attagaagac aacgctggaa cgctcggttg
     8281 tttggcattg gctcgagaag cggtcttaca ttcataaacg aaaaaaggct cttcgacatt
     8341 tagtgttggt agagaaagat acaactgaaa atttagtcat aaccagttag gcagagtgct
     8401 taactggttt ttgttttact tggaagatca agccttgaat gagataaatt cttgctcgaa
     8461 agttatggaa atttcgatag ccgtaggcca cttgtattgt gcaatagttt tttacacgcc
     8521 gtttgtataa ttaagcgaga caaataaaaa agccatttat gttacactca aatcgtaata
     8581 cccgctaaag catacaagga gagtgaacat aaatgactta tacccatctt acaccaaacg
     8641 agcttgtaat gatagaagca tattttcatc aagaaactcc ggttgctatc gttgcgaagc
     8701 agcttaaacg tggacgccaa acaatttaca atgtctataa ctttctcaaa tgtggtggaa
     8761 cagcacttga atgctttgaa caatacaaag aaaataagcg acgttgtggg agaaccgaaa
     8821 tcatttttcc tgctgaggaa aaagaataca ttgcaaaaag atcaactgaa ggttggaccc
     8881 cagacgtcat tattggccgt gcagagcgaa ccttctcttg ttcagtaagt accctctatc
     8941 gccggt
//
LOCUS       DACDTO010000103         8864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDTO010000103 DACDTO010000000
VERSION     DACDTO010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.103"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(271..2652)
                     /locus_tag="IWK70_002500"
     CDS             complement(271..2652)
                     /locus_tag="IWK70_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9614999.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2642..3289)
                     /gene="recU"
                     /locus_tag="IWK70_002501"
     CDS             complement(2642..3289)
                     /gene="recU"
                     /locus_tag="IWK70_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9615000.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3353..3892
                     /locus_tag="IWK70_002502"
     CDS             3353..3892
                     /locus_tag="IWK70_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9615001.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3982..4401
                     /gene="gpsB"
                     /locus_tag="IWK70_002503"
     CDS             3982..4401
                     /gene="gpsB"
                     /locus_tag="IWK70_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9615002.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4423..4790
                     /gene="rnpB"
                     /locus_tag="IWK70_002504"
     ncRNA           4423..4790
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWK70_002504"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4999..6165
                     /locus_tag="IWK70_002505"
     CDS             4999..6165
                     /locus_tag="IWK70_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9615003.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6211..7707
                     /locus_tag="IWK70_002506"
     CDS             6211..7707
                     /locus_tag="IWK70_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9615004.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7788..8471
                     /locus_tag="IWK70_002507"
     CDS             7788..8471
                     /locus_tag="IWK70_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9615005.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2805 a         1591 c         1874 g         2594 t
ORIGIN      
        1 caatgggatt attaagaagt ggcactaaat aaaagactag tgtgtattta gccgatttaa
       61 ttataaacaa gtaaatggat aaagtatatg taatgataaa agaaaaatgg attttctaat
      121 ttttaagcta aatgttagat cacacatgta tatgctggat agtcttataa aaaatcatgt
      181 aatacagctt ggtacatgat ttttatttgt agctgttttt tacatagaca caaaaaagaa
      241 accacgcttg tagacgtggt ttctttttta ctatctgttt ccagaagctg aggtgctgct
      301 ggatggtgta tggtcatttg cagcatccgc tccgctagtt gctggctcac tggaagaagc
      361 aggaggagca gatgaagacg gtgtcgagct ttcttcggat gaagaagaag gaaccgttgt
      421 tgaagacgct ggctcgcttg tttctgctgt cgtgctttct tttgaggact cagcagttga
      481 ttcgctttga ctagtagagc tagagctttg agtcgttgtc tctgttgttg agcttccagg
      541 agtctggaca tagctggaag atggaatagt tgttgatggt gtaattgcat tcgatctagc
      601 tgtatattga tctttgtagt acagttcacc gcccacacga attaatccgc ttggcatctc
      661 ccagtctgta tttgtcacgt tagctgaaac atactgcata agctcgcgat aaacatcaga
      721 ggctacgtgt gacgattctg aagtgacagg tgtcattttt ttattgtaac ctgtccacac
      781 ggaaatcgaa taatttggcg tatagcccgc aaataggata tctggataca ccccagaaga
      841 aataccaagt tttgcatact cgtcatctgt gtaattggat gttccggtct taccggcttg
      901 atataaccca gcgatttgcg cattcgtacc agttccttca gtgatcgtat cttttaaaat
      961 gtctgtgatc atataagctg tttctggaga catggctgtt tttccatctg gctcataagt
     1021 ttcttctgtt ccgtcttgga agacgatttt attaacatat tgaggtttgt aataagtccc
     1081 tccattggcg aaagccgcat aggctgctgc catttttaag gaagaagctc cgtattttgt
     1141 cccatcttgt tcttccgtat tactagagat cgcattcgat tgatggatcg tactgtattc
     1201 gatcccaaga ttttttaaga aagaagccac tttatctgaa ccgacttcat tgaataattt
     1261 gactgctgga acattacggg ataagtaaag tgcttggcgt aaagtaatgg tgcccatata
     1321 ttgattatcc cagtttccta caggagtcga agtcccttca tagttatacg gtgcgtcagt
     1381 aatagttttt ccagtagaat atttcaaata ttcaaaagct ggtccgtaat ctgtgacagg
     1441 tttcatcgta gaaccaaaat cacgggaagt attcactgct aagttgttgc ctaaagttac
     1501 gtcttctgcg atgtgacgtc cgccaatctg ggcttttact ttcccggtat tggtgtcgat
     1561 aagggtggag gcaacttgca tctcgtcgtc tgggtaagag acatactgat ccgtgttgac
     1621 gatatcatat agtttctttt gggcgtccag atccaaattc gtataaatct ctaaaccgtc
     1681 tgtgtagaca ttcttatctg ttttttcttg tacttcattg atgacttctt tgacataatt
     1741 atcgacgatc ttagtattgt catcggattg agttagttct tgcagaccat ccgttaccgg
     1801 tacattaaca gcttgatcat attctgtttg agagattttt tcgttttgaa gcatcgtata
     1861 caaaacagta tctcgtcttt tcttcgcctg atctggataa acataaggat cataggcgga
     1921 aggggcttga ggcatcccag caagcaatgc tgtttgcggt aaagaaagtt ctgataattt
     1981 cttaccaaag tacatctcag aagctgtctc catcccgtac aagccgtttg acatatacac
     2041 tttatttacg tagtaggtca agatctcttg tttcgatttt ttttgttcca atcttacagc
     2101 catccacgct tcttgggcct tgcgtttcag agtctgatcc tcggcactgg tggagaagaa
     2161 ggaaagtttg atcaattgct gtgtcaaagt acttccgcct tgcagtccgc cagaggtaaa
     2221 gttagagagg gcagaaccga tgatccgaat agggtcaacc ccgatgtgtt tataaaaacg
     2281 gcgatcttct accgacacga tcgcatcttc taatgttttc ggtaattcat tcgcagaaat
     2341 tttttcacgc ttttctgcgc ctaaatcttc gaatagttcg ccatcttgtg tatatagttt
     2401 ggaagatacg gtagcgtcta attttttatc cgtcaattcg ggcgcatctt ttgcgtaata
     2461 ccagaaaagt cctacacctg ctaaaaagag gatacagaaa aatgagagta ttcctaaaag
     2521 gatcttgata aataatcctt ttttatgtgt tccagaggac tttccagaac ctttgccaga
     2581 attcttcttc acactctttt taggagtagg ctgtttctgt cgtctagagc ttctagtttg
     2641 ttcatttgcc attcttgctt tctccttcaa tagataatgt tgataaatag aagtcaaccg
     2701 cgtccaaata aggaatccgc ggagaaatac cagttttgat ctctattcct agttcttcga
     2761 tcaaagataa aggtagtgat ttgcgtcctt tttccttttg ggcgtcccag taagcaatca
     2821 aatgaacact attcaaataa aaacagcgct tcaaagacga aaaccataac aagacaaagc
     2881 agaccccatt ttgttcgagg catgctttca tatgttcgat ttgatgggca tggaagtttt
     2941 taaagggaaa agatgttttg ttcttggttt cttttgcttc aaagtccaag taaaagcctc
     3001 gatagacgcc attatagtct gtagttgagg cttgggtaaa gtaggcttcc ttgatcacag
     3061 cagcacttct cttaggatag tctactttca cgatttggat aggggtaggc tttttatgga
     3121 taaccgccaa cccgcgggaa agataatatt tattactttc attgattgct tcttcaaagc
     3181 gcatcccccg attgccgaat tcagtgatct gatttctttt ttcttgaatt ttttcgacgg
     3241 gagaggcatt cttataataa ggttggccat ttggatagcg caataccatc tttaatcaca
     3301 ctcctaggcc acattatagc aaaaaagcgg atagaaagaa aaggaatctg agatggataa
     3361 gtcaaaagta atctatgtgt ctgggtatcg aagtttcgaa ctcggtgttt ttaaagaaaa
     3421 tgatcctaaa atccaagttg ttaaaaatgt tttaaaaaaa gaactccatc agttagcaga
     3481 agaagggctg gaatgggtac ttgtcagtgg gaatttgggc gtcgaactat gggctgtgca
     3541 aatagtagcg gaattgaaag aagaatatcc ggaactatct cttggtattt tgtatccttt
     3601 ttctgatttt ggtaataact ggaatgaagg gaaccaagaa aaaaaacaat tagcagaaca
     3661 attagcagat tatgtcgaag ctgttagcca tcaaccctac caatcaccta gtcagctaaa
     3721 gaatcacact cgttttatac ttgagcatac agtgggctgt ctgctgatct atgatgaaga
     3781 attccctgga aaaacgaagt atttttttga agatgcacag aattaccaag cagatcatcc
     3841 ctacgaaatt cggctgatca gtatggacga tcttcaaaat tttagtgaat aatcgatatt
     3901 gtgtttgatt tatcaacttt tttcctatac aatgaaaaag gtagaaatgt tattacgtaa
     3961 atgaaaacta gaggtgtaaa tatggcgaat ttggtttata gtcctaaaga cattttacaa
     4021 caagaattca aaacaaaaat gcgcggctat gatcccgttg aagttgatga attcttagac
     4081 aatatcatca aagattatga agcttacagc aaagaactcc ttgctttaca agaagaaaac
     4141 gatcgattga gtgcaaaagt agctcaatta tctaaaactc aaggagcagc tcaaacacgc
     4201 gtgcaacaaa cagaggcacc aaagagcgca gcagtgacca attttgatat cttaaaacgc
     4261 ttgtcaaatc ttgaacgtga agtatttggt aaaaagcttg atcaacaagc aagtgcagtc
     4321 aaaccagcac aacctaatcc aaataactac acaaatgcag acacaagctt agatgataat
     4381 gaaaaaacac gtcaatttta atttttgatt aatgaatttg cggttttcgg gtaatcgcgg
     4441 tctgacttga tcaggctgag gaaagtccat gctcgcacag gctgtgatgc ctgtagtgtt
     4501 cgtgcttagc gaaaccataa gctaaggtac tctttggagt aacggcagga aaacaagcta
     4561 aggcttcggc tatgcttaag tatccttgaa agtgccacag tgacgaagcg gctggggaaa
     4621 cccatccggt ggaacgcggt aaacccctcg agcgagcaac ccaaacaatg gtaggggcgc
     4681 tttttgtctg gaagaagaac aggacaaaaa ggcagtataa ctgcagatag atggttaccg
     4741 ctttgttttt tctccctgga aaagcaaagt acagaacatg gcttatagaa aaccgcaaac
     4801 atcagggaat taggtcgtga caaaagtaat gcgttataag aaacaagaaa aacatttgaa
     4861 agctattttt caatacttca gatgaatcgt tcttattaca aaacaaactt ttgtatcacc
     4921 ggttattcgg aatttgagta aaagtgtggg gtaggactga tgtcccacct ccttttttta
     4981 taaataagga gattgtgtat ggaaaaaaac aggaagttca acctcgtcgc aactgcggca
     5041 agcgggctag aagcactggt tggtaaagaa ttaagagatc ttggaatcga atgccaagtt
     5101 gagaacggac gtgcacgttt tgaaggaaca atggaaacga tcgctacagc taatttatgg
     5161 ttacgtacag cagatcgagt aaaaatcgtg gttggggaat tcgatgcgta tacgtttgat
     5221 gaattgtttg aaaacgtaaa agcattgcct tgggaagatt ttcttccact agatgcagct
     5281 tttcctgtag ctggaaaatc gatcaagtcg aaactatata gcacacctga ttgccaagcg
     5341 atcacaaaaa aagcaatcgt tgaacgtctc cgtacctatt atcaccgtcc agcttctgta
     5401 ccactgacgg aaactggcgc acattttcag ttggaagtag ctttgctgaa agaccatgtg
     5461 atggtaactc tggatacgac aggaccaagt ttattcaaac gtggttatcg cttagaaaaa
     5521 ggtggagcac cattgaaaga aaacatggca gcagcactta ttatgttgac aaattggcga
     5581 aaagaccgtc cattttatga tcctgtatgt ggttcaggta cgctttgtat tgaagcagcc
     5641 ttaataggcc ataatatcgc accaggattc aatcgtgagt ttgcttgcga atcttgggat
     5701 tggttcagtc aagaaatcat ggaaaatgtt cgatcagcag ccgaagaaaa agcagattat
     5761 gatattgaat tagatattac tggttcagat atcaatggac ggatgattga aattgccaaa
     5821 gccaatgcag aagaaatcgg attaggctct tcgattacct ttaagcaaca agcagtcaaa
     5881 gacttcaaaa cggataaaga atacggggtc atcgtggcta atccgcctta cggagaacgt
     5941 ctaggagaag aagaaactgt tcggaaatta tataaagaaa tgggcgatgt attccgtcca
     6001 ttaaaaacat ggagtaaata tatcttgact agcgacttag cgtttgagga attttacgga
     6061 caaaaagcaa ccaaaaaaag aaaattgtac aatggtgcat tgcgtacgga tctattccag
     6121 tattggggaa ctcgtccgcc tagaaaaaaa gagaaagacg aataatctta ctaattgatt
     6181 cattcaattg gatgtttgga ggaataggga atgaaagaaa aagagttttt acaagaagta
     6241 aaagaaatca atcttctaca acaagcttta ggaatcctcg attgggatac acaaacaggt
     6301 atgcctgaaa aagctagtgc ataccgaagt gaagtagaca gctatcttta cagcatttat
     6361 ttttcaaaaa aaatcggtcc aaaaatcaaa gaagccattc gctatttttc tgaacatccg
     6421 gaagaattgt cggaagttgg aactgccgtt tttgccaaag taaaagaaga gtatgatttg
     6481 gaacatcgcg taccggaaga tcttatgaga aaatatacag ctgccgtttc ttcagcacac
     6541 ggacaatggc aaaaagctag agaaacacaa caattttctg attttcaaca cgcattgaat
     6601 caaaatattg aattaacaaa acagttgata ccttactgga gaaaagatga aaagacgaat
     6661 tatgatgttt tgctgaatca atacgaaccc gggatgactg tcgaaatcct tgataaggta
     6721 tttgatcagc ttaaagaagg aattctttcg atccgccgta cgctccaaga aaaagggact
     6781 gaacctgaaa cagacttttt aaatcggaga atgacgaaaa aacaacagaa gagatttgta
     6841 gtgaaagtaa tcgaacaact tggttatgat tttagtcgtg gtcgtttaga tgacactgtc
     6901 cacccattta tgactggaat taatccgaat gatgcacgaa tcacgacacg atggaacgaa
     6961 acagatttca aaatggctgt ttttggagtg atccacgagg ctggtcatgg tatgtatgaa
     7021 caagacatcg atgataaata tgcgtataca ccggtttatg aaggtacatc aatgggtatc
     7081 catgaatcac aatcgttgtt taacgagatc attattggaa gtagtcgcag cttctggaaa
     7141 aagcaatatc ctttctttca agaatgtgca gaaggaacat tcgatgatat tccttttgac
     7201 aaattttatc gttcattgaa ccatacaaaa tcaagtttgg ttcgtatcga ggcagacagt
     7261 ttgacttacc ctctccatat catcattcgt tatgaaatcg aaaaaatgat tttcaatgaa
     7321 ggggtagatg ttgctgaact acctgaaata tggaatcaaa aatatgaaga gtatctggga
     7381 atacgaccaa caaatgatgt ggaaggcatc ctacaagacg ttcattggtc aggtgcaagc
     7441 tttggttatt ttccctctta tgcattaggg tacatgtatg cggcacaatt acttcattct
     7501 ttgcagcaag aaatagatgt ggaaaatgtt ctagcgtcag acgattattc tccaatcaaa
     7561 aactggatgt cagagcatat ccatcaatat ggtgcatcta gaaaaccaaa tcaattaatc
     7621 atggatgcaa cacatgaacc gcttaatccg caatacctga tcgactatat gaaagacctg
     7681 tattttaatg tatatcaggt aaaataaaat acaatttaca aagatagctg aaattcatct
     7741 aatctataga tgttttcagc tatttactta agaaagaagt ggaacgaatg gataaattac
     7801 aagtgatcgc tgattatagt atggaccaat tagcgcatga tcaaacagga catgggagcg
     7861 atcataccaa aagagttgtc aagttagctg aaaaaatttt ggagacggaa ccgcaggcag
     7921 accgctttgt gacgcttgca gcagcttacc tacatgatac gattgatgat aaagtcgtta
     7981 aaaatgagaa cgaggcaaag caacagcttc gtgtattcct tcgctctttg ccaatagtag
     8041 aggaacagat ctcgatgatt ttttccatta tcgaaaatat gtccttttct aaaaatctct
     8101 ctgaagcggc cgaactatct ctggaaggta agatcgttca agacgctgat cgtatcgaag
     8161 cattaggagc aatcggcatt ttgcgtactg cttatttcgg cggtggacat gggcacccga
     8221 tttttgatcc tgaactgtat ccgcaaacat tcaaggacaa agaaaattac cgaaaaggca
     8281 cgacagtgat caatcatttt tatgaaaaac tgttcttgct gtctgataaa atgaatactg
     8341 attacggata tgaagaagcc aaacgaagag aaaccttcat gcgtgaattc ttagaagaat
     8401 tttttctgga atggtctatt gacgatgaaa attttactcc agacaattgg atcaaaaata
     8461 acaaaaaatg atggaggttg tgacagaagt ggtcagctcc aagatatttc ggatcatttt
     8521 ccgtaaaagt tacttctgga acactgttta ccaagaggtc gtgagcctgc cgttcagctc
     8581 cgagtcacaa ggaacaattt caaaaaatag cttctcgtgt ttgatgaata ttgtaacttg
     8641 tgattcggaa ttggcaggag aacaccgttt attcggaaga ataggactgt gacaagactt
     8701 ttgtcacagt ccttttttcg aattcaacaa gtaaaacttt taagaaatat tcagagagta
     8761 aaattaaaaa ttgctgataa acgaagatgg aaattttatg tatttttatt ggtctatgca
     8821 ataaataaat taaatattca gaatacaatt gattttaacg aaag
//
LOCUS       DACDTO010000104         8851 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.104, whole genome shotgun sequence.
ACCESSION   DACDTO010000104 DACDTO010000000
VERSION     DACDTO010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.104"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            63..1277
                     /locus_tag="IWK70_002508"
                     /pseudo
     CDS             63..1277
                     /locus_tag="IWK70_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(1456..1791)
                     /locus_tag="IWK70_002509"
     CDS             complement(1456..1791)
                     /locus_tag="IWK70_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9615006.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(1775..2047)
                     /locus_tag="IWK70_002510"
     CDS             complement(1775..2047)
                     /locus_tag="IWK70_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9615007.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2498..3301)
                     /locus_tag="IWK70_002511"
     CDS             complement(2498..3301)
                     /locus_tag="IWK70_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9615008.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3279..4403)
                     /locus_tag="IWK70_002512"
     CDS             complement(3279..4403)
                     /locus_tag="IWK70_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9615009.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4419..5234)
                     /locus_tag="IWK70_002513"
     CDS             complement(4419..5234)
                     /locus_tag="IWK70_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9615010.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5224..6105)
                     /locus_tag="IWK70_002514"
     CDS             complement(5224..6105)
                     /locus_tag="IWK70_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9615011.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(6181..7470)
                     /locus_tag="IWK70_002515"
     CDS             complement(6181..7470)
                     /locus_tag="IWK70_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9615012.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7698..8342)
                     /locus_tag="IWK70_002516"
                     /pseudo
     CDS             complement(7698..8342)
                     /locus_tag="IWK70_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2742 a         1883 c         1410 g         2816 t
ORIGIN      
        1 caaaatcaat tctaccttag atgttcttat atcactctat cagtcctatt tgacaaagag
       61 ccaagaaaaa gacgttggtc gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa
      121 gttgtggttc tactgtggta gatggaaacg ggaaagcaat tatagtgaaa aatgggaaga
      181 aggaaacgat tgtccgtttt gaacaataca atcatatgcc tttggttacg cgcctaaaaa
      241 agcaacgcta tacctgtaaa aactgtcgta cccattggac ggctcaaagt tattttgtcc
      301 gactcagcca ttcaatcgca gatcatgttc gatataaaat tgcttcttta ctgactgaaa
      361 aagtatcttt gtcttttatt gcgaaaagtt gtcaggtatc tttgactacc gttactcgta
      421 cattgaaaga gtttaaaagc tatttaccaa agccttctaa gaagattctc cccagagtat
      481 tgatggttga tgaatttcgt tcgcacgctt ccatagaaga taaaatgagc tttatttgcg
      541 cagatggtga aacaggaaac ttaattgctg tcttgcctac gcgtaaatta cctcgattaa
      601 caagctattt cttaggctgc accaatccag aagaagtaga attcttagtc acagacatga
      661 acgccgctta cttccagctt accaaacgtg ttctgccaaa tgcgaaagta gtgattgatc
      721 ggtttcatat tgtcaaacac atgaatcagg cactcaatga gttgcgtatc cgtgaaatga
      781 atgagcttcg taaagcagga cagaaaagtc aggcagaaaa actgaaaaag aactggcgct
      841 ttctgctaaa aaatcgtgca agcatcaacc attatgagta caaaacatgg aaaagtttcc
      901 gagcaccaaa atacccattt ctcactgaag caatgatgat tgatcgattg cttgagtttt
      961 ctgcgcctct aaaggaggcg tatccctttt ttcatgaatt agtcgaagcc tttcgagaca
     1021 aagaccctga cttatttttc tccttattgg cagaacttcc cgaaacgtta gatgacggct
     1081 ttcgggagaa gcttcaaaac cttctgacct atgaagaagg tatcaccagc gcaatgatct
     1141 atccttattc caatggaaaa attgaagcga agaataccca cataaagaca atgaaacgag
     1201 tatcctacgg atttaaatca ttcgagaaca tgagaattag aatctttttg atcaatcaat
     1261 taatcaatgt aagataacaa aaaatctgaa ccagaatgtg gttcattctg acccggattt
     1321 gattttgcac aactcatcag tccttattga caaagagcca aaaatctgag ccagaatgag
     1381 gttcattctg acccagattt gattttgtac aacccatcag tccttattga caaagagccc
     1441 ttctttttag tattattata attcttccat gcgttcttcc agtcgctgta aatcgcttgg
     1501 acttaattcc ccaatttttt tccatttgac ctttttgttg tttttgggta attcgtttag
     1561 agttcctacg tcaatccaag attctttttt tagtcctgcc tgtttccaat cccttatttc
     1621 aacgtatttc agctttattt tatcggattt agtatgatat tttgaagtaa ttgacagaaa
     1681 cgaaattgtg tccagtccat tttttagaat atagacaggc cggcgtttac cgccctctgc
     1741 agctgtgaaa gcaacgtaga cggttgctat atcattagta ttcatcttca ttgaaccatt
     1801 cttcaatttt ttcgtcagtg tctagttttt ggacaggcaa tttttccgct aatctttgaa
     1861 tattatggat tcgtttactt ttttccgaca attcaataga aaatgggatt tttccttgct
     1921 caacaatttg cttataaaaa acggtgacgg ctgttttggg gctcatgcct agttcatcaa
     1981 taatgctttc tgcttcattt ttcaattttt cgtccataga aatttgtaac ttagccattt
     2041 tctccacctc tttctgacaa acacattata tcacggaaag agttcaaatg gaattaatac
     2101 aactactttt gaactccttg aaatgcagtg tatttatcta aatataagga aaggatatag
     2161 tgctaaatgg ccttgataga tagaaaagtt tcaaaatccg ccacagcgat aattgaagcg
     2221 aacactcaac gacacacaga acaggaaaag actcctaaaa gaaccaaatt aactaggacg
     2281 tgtctgaaaa ctccacaaag atgtggtatg ctctggtaaa aaacgaatgg cacaaccacg
     2341 aagaccgata tggttcttgg aaaactttct ccaccctttt tgtaattgga gggatagtgg
     2401 tttattgata gcgattttcc aagttcttca agtggaacca gacattgagc accacggcta
     2461 aaaagtgaat gaagtcttta tttttaatca ataattttta atgattacca gtaattggtt
     2521 ttttcatgcg ccctatttct atttgatgtg gagttaaata atgctgataa gtaaaaattt
     2581 ttccgtcaat taaactgcca ctagtgaatc ctgttctatt ggtaaaaaga gaatagtctg
     2641 ggtattcatc tacaccaaat gataaagatt cagccataaa ctgtttttta ccagcaaatt
     2701 cagcactgct accttgatgg atatgtccta caaaaatacc taaaatatca ctttcggcaa
     2761 tcaattcagc aaaaccttcc ggaacttcag ttacaataga tggatcttcc cacgacaatg
     2821 gatgatgaac caataaaatc gttccctttt cagcaggtgt acccaaccac tcttgtaatt
     2881 gaatcatctg tcctatcgat atttttcctt cgtgctgggt atcttgagca gaatccaagg
     2941 aaataattcg taacccattt tgattcgtac aatggatata ctctcccgtt tcattgcttt
     3001 caacactcat tccttcaaag aaaacactgc gacgatcatg attcccacga caaaaatagt
     3061 aaggtgtggt tgggaaatat tgatccaaca ttttccgtaa gacttgataa tcagaaatcc
     3121 ccccttcatg tactaaatca cctgttaata gaataaaatc gtacactgtt aacgagagtt
     3181 cttgtaacaa tttttccaat gccaactgtg gatttttaat tgttggaaat agttcagcag
     3241 taaacaagtt agtttgatag ttttctctaa aatggatatc agagagatgc aaaaagcgca
     3301 tggctattcc ctctttcttc aataaaataa tcaggatatc ctaaattttc ttgcgtctga
     3361 taatcaaaaa aatgcattct ttcaggttct atgcggagat tgactgcatc accaatctgc
     3421 caagtaggtt tttcattaag gacaatcatt tctataccat cgactttaac ggtgtacgca
     3481 taactttgac ctaatagctc acgatacttc acttgaccgt gtaaatcgct tgtttctttt
     3541 gaaaaggtta cgtgttctgg tcttatgcct aaatacaatg agccactaac ctgaagaagt
     3601 tgtttttgcc aagctggatt caatgttaaa aattgactgg ctacctttaa tgttccttga
     3661 tcataagtaa ctggcataat attcatgcct ggcgaaccaa taaaatttgc agtaaaagta
     3721 tttttaggtt tcgtataaac atttttcggc gtatcaatca tctgaattct accttcattc
     3781 aataaaacaa tccggtcagc taacgtcatc gcttcaattt gatcatgagt gacgtagaca
     3841 aaagtttgtt tataacgttg atgaattttt accaattctt ttcgtgcatc caatcgtaaa
     3901 cgcacatcca aatttgataa cggttcatct aataaaaaat aattactccg tttaaccacc
     3961 gcacgagcca atgctacacg ctgccgttgc cctcctgaca aatctttagg aagtcgatct
     4021 tggtatggcg ttaaacctaa catcttcaaa gcttcttcca aacggtgctg aatgtcagct
     4081 gtcttaattt tattcgccct taatgcaaaa ataatattct cctttacagt catgtgagga
     4141 tataaagcat aattttgaaa aaccattgcg atctctcgtt ctccacaagg tagcttattt
     4201 gctagttgtg tacctaaaaa aagctctcca ctcgtaattt tttccaaacc agcaatcatt
     4261 ctcagcaaag tagattttcc tgaaccagat ggtcccagta acacaattcg ttctccagcg
     4321 gccacagcta aatcaatttc ttttaaaatg gtatgttctc catatttttt tccgatattt
     4381 cttaattcaa gcgtttctcc catgatttgt cctccaattt atttaatacc tgcagaaaca
     4441 aaagtcccga taatatattt ttgaaaaatt gcatacaaga ctaacggtac aaacattgta
     4501 attaaactaa tcgccattgc tacggtaaaa tttgtcccac cttcagaact aatataggtc
     4561 tgcatcgcca acggcaaagt ataattatct actgtccgta aaattaatac cggccaaaca
     4621 tactcattcc aagaggtaat aaaaaaccaa ataccggtag aagtaatcgc atgtctagta
     4681 agaggaaaaa caattttact catgacggtt gtttgtttga tgttatccaa tgccgccaca
     4741 tctagaaggc tcactggaat ttcccgcatt gtttgcgtca ataaaaaaat gcccatcgca
     4801 tcagcaaatt gtggtattat cacaccccaa gtagtatcaa ttaaattaag ttttgcaatc
     4861 aacaaataat taggaatcat cgcaacagta aaaggaatga acatcgtacc aatgatgata
     4921 aagtaccaga ttttcttccc tttgattcgg caataagtaa aactatatgc cgcaaaaaaa
     4981 gctacgccta acttaacaac cgttataatt gtagcaataa aaaaagtatt tctcgtaatt
     5041 ttgataattg gaagattttc gaacaaatag caaaaatttt ccaaggtaaa cggctgtggt
     5101 atcaaactag taatcgtctg ataggcatct ttcaatggct taaaagaatt ggccataata
     5161 aacaataatg gatagataat aatgattatg atgaacataa ataagagatg tggcaaccag
     5221 cgtttaattt tcatagtaaa cccccttttc cacaaattta aatattaccc ctaagaaaac
     5281 agcaaatatt aataaagtta aaactgaaat tgctgaagat tctccgactt tatacatttc
     5341 aaaagccttt tgataagcat aatagatcac gtttgcacta ccattattcg gtccaccacg
     5401 agtgatgatc ttaatcggag taaacacata ctgcagacct tgaacaatgg tcatcaccaa
     5461 taaatatagt cctgtcgaac tactcatagg caacacaatt gtaaaaaaaa tgcgccagat
     5521 gggtacacga tccaacttag ctgcttcgat catctcttga tcaattccca caattgctgc
     5581 aaaaatgaca atgaaattat aaccaaaaac tttccaagca gtaatacctg caatcacagc
     5641 aattacccaa ccttgtaaat tcatccacat aggcagtctt aaaccaaaat ttcccaacaa
     5701 gacagcaatt ggtcccgata ccggatttaa tatccatgta aaaaccatcc ccgccactac
     5761 caaagaaatc acagctggaa taaaaaataa tggtttatac aaattctgcc ctttttctat
     5821 aactattcct aataaaaagg atgtaatata tgggaacaca cagctcagca agacaaatag
     5881 aacggtatag gacaaagtat tcttcaacac tttatgaaag actggatcag tcagcacctt
     5941 tacatagttg gcaaagccaa caaattcttt agtgggggcc accatattcc agtcaaaaaa
     6001 actcaaatag atcgtataaa tcaatggata aaaaacaaac acgcccaaca aaataattgc
     6061 tggcgccaga tacaaataac ctacatagcg cttcatttcc gacagcctcc tcatccttat
     6121 gtcaaaagca gacgaccttg tttttccttt cttacaggcg ctgtcgtctg cttgctttgc
     6181 ttatttgagt aactcattaa tttccttttg agtagctttc atcgtttcat tgacggaatt
     6241 acctgataaa attttatccc gcatatccaa caatttttgt tcagcttcta atccagcatc
     6301 tccaggaaat gaactccaac tgaccatctt atccatctga tcaattgctg gtatcatcat
     6361 ctgattttca gctaagaaag cttttaatcc attttgggcg tctgcaacat ctttacgtgg
     6421 tggtaaataa ccagtacctt ctgtccattt agctaaagat tcaacactat aaagatattt
     6481 taagaaatcc caagttgctt cctttttctt atccgtttgt gatgtaacag ctaacattgc
     6541 gccaccagca ggtagtttaa cttttttccc ttcccaactt ggtgactcaa ctgctttagc
     6601 agtaaaagta ctattttttt gaatgttgtc cctttgagca atagtggtat agaccatcgc
     6661 gacgttacca tcaataaagc tttgcacacc ttgttcccac gtagtgtgta atgccgtctg
     6721 gtcttttacc atcctttgat ataattcata agcagcgata ccatctttag aagcaaaggt
     6781 agctttgcca tcttttatta cacttgcccc attactttct aaaattgctt gttgtgccca
     6841 attatcagca ggttcttgaa tatacacgcc atactttcca gttttttctt ttattatctt
     6901 agaaaaagtt tgaacttctt cccaagtttt tggaccattt tcatccaagc ccgctgcttt
     6961 taatatatct tgatttaaat ataaaaccgg attactaatg gagtaaggaa tacccacttg
     7021 tttaccatct ttttcagaca gtgccatgat attgtctaaa aaatgatctt taaaataatc
     7081 agcattatcc ggatccactt cttctactaa agattgagga ttagtatatt tgaaattttc
     7141 tgcaaaataa tctttaaaag cccatcccac ttgtactacg tccggtattt ctttggaggc
     7201 aacagaagct tgtaaatttt gcatcaagcc tttatacata tcaggattat attttgctac
     7261 aaccttcact ttgtcttgag attgattgta tttatctact aattcattca ctgtttttcc
     7321 acctagggta tccgcattaa catgccaata ctctatcgtt acacgaccct ccgatttttt
     7381 tgttgtcttg gtattcccac taccacaagc tgccaaagag atgacactta aaacagacac
     7441 aacccctata acaagatttt tctttaacat aacattaact tcctcctaat ttctttatgg
     7501 ccatttttct aatcaagaaa tattatgaac taccaatgta aatttctagt taattttctg
     7561 taaataattc gaaatttgtg catttcgggg tacagggtca ctggacatat ccatcaagct
     7621 aagaataaag cccccatatg gtcatgtatc ccatctccaa atctcttaac tcctctgttt
     7681 tgattataaa atagtttatt ctgtgctcct ttcttccata aataggccca tgcacaacgt
     7741 gaggttatat tataaacacc acagcgcgca aggtactcaa tcgtttctag ttcattgact
     7801 gtccatacag aaaatgtcaa tcctagctgc tgccccactt cacgggccaa cttagttaac
     7861 catccttcat gaatattgac gatttgaacg ctttgatagg ctaatttctg taaggtatcg
     7921 atcaaatctg cttcggtaat atccccaagt tttttccctt cgaactgatt ttcaatatta
     7981 taaaatatat gctttcccca aatcgcagcc caagataatt gtacatagcc actaagaaca
     8041 atttgtggcc agatttgata ggtttgtgcc agcgaaaaaa cggccatttc caatccctct
     8101 tcttttaaat cacagttaat ggtcatggtt gccttctcat tggccactgc ttgaaacacc
     8161 tgctcaaggg ttaccgaatc tgggacaact tcttacctgt catgggctag tattagcgta
     8221 ccatctgttt gctgacgcac atctacttca aaacaaagct ggcgatccaa acaaaagcga
     8281 agaaatgccc agctattctc ctccgtattg tcactaccag agtgtgcagt gatcatggtc
     8341 attttgaccc ctcctttaat tccgtgattt tctcccacac gtccactata ccgatgaagc
     8401 gttaatttct tgttaatttc ttgtaaattc cctgcgtttt ttcagtaaac tagttgtaaa
     8461 tcttgcatgc tccttcctac gaactcatga atcgtcgatt ttgcccttct agctagtttc
     8521 accaattaag tagttctaca ttccccgctt ttaacctact acaccttttt ttgttttcag
     8581 ctattccttc ttcttgctga aagtccttga ggttctttct tacaggatac catcaaggtg
     8641 ttcaatggct tattcccatt gaatgaccga gacttactat cagatttctg ctcgttgaac
     8701 caagtctttg atacaggatt tcttccgtca tcttctcaca aataccacct tgcgtttctc
     8761 tagcgattgg tcgatgtgca ccctcccagt ggactgtcac tacccctttt tgctatgcct
     8821 agcccacaaa aaaagctgtc ctcacagaca g
//
LOCUS       DACDTO010000105         8731 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDTO010000105 DACDTO010000000
VERSION     DACDTO010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.105"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..194)
                     /locus_tag="IWK70_002517"
     CDS             complement(<1..194)
                     /locus_tag="IWK70_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615013.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHV"
     gene            complement(379..876)
                     /locus_tag="IWK70_002518"
     CDS             complement(379..876)
                     /locus_tag="IWK70_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9615014.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1006..1716)
                     /gene="budA"
                     /locus_tag="IWK70_002519"
     CDS             complement(1006..1716)
                     /gene="budA"
                     /locus_tag="IWK70_002519"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9615015.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1729..3393)
                     /gene="alsS"
                     /locus_tag="IWK70_002520"
     CDS             complement(1729..3393)
                     /gene="alsS"
                     /locus_tag="IWK70_002520"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9615016.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3601..4263)
                     /locus_tag="IWK70_002521"
     CDS             complement(3601..4263)
                     /locus_tag="IWK70_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9615017.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(4273..5088)
                     /locus_tag="IWK70_002522"
     CDS             complement(4273..5088)
                     /locus_tag="IWK70_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9615018.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5349..5792)
                     /locus_tag="IWK70_002523"
     CDS             complement(5349..5792)
                     /locus_tag="IWK70_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9615019.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5926..6264)
                     /locus_tag="IWK70_002524"
                     /pseudo
     CDS             complement(5926..6264)
                     /locus_tag="IWK70_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(6252..6629)
                     /locus_tag="IWK70_002525"
     CDS             complement(6252..6629)
                     /locus_tag="IWK70_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9615020.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6853..8046
                     /locus_tag="IWK70_002526"
     CDS             6853..8046
                     /locus_tag="IWK70_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9615021.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(8212..8634)
                     /locus_tag="IWK70_002527"
     CDS             complement(8212..8634)
                     /locus_tag="IWK70_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9615022.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
BASE COUNT         2578 a         1753 c         1454 g         2946 t
ORIGIN      
        1 taaacgtgag tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg
       61 acgtcatgat attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct
      121 ccaaccactt tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata
      181 gtttcgttat tcatatttca tccatcctta atgttttttc attattcaaa atgataatac
      241 cattaaaata tgattgtgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac
      301 cgaaaaacag aaatgaccac tgtctttccg gctcttttta ttcttgattt ctcttctttt
      361 ttgtttcaat agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc
      421 taataaaaga tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt
      481 tactccgcaa tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac
      541 gatttctgat tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc
      601 aacgatcaat gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat
      661 acgtgtgcaa gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa
      721 ataatatcct gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc
      781 taaatttgct aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat
      841 ttcgtatgca tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc
      901 tatggagaag attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg
      961 agatacgtca ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg
     1021 gatttctgct gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac
     1081 tggaaaatgc tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc
     1141 aaaatccatt acatgtccgc caaatgtatg tgtatcatcg ataaaatgca ggtgaaatcc
     1201 cgctgcagca actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggat
     1261 attgctttgt tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt
     1321 ttcttgcttc ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc
     1381 caaaaatagg ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc
     1441 tgcagttttt gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg
     1501 ggtaagttca cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac
     1561 gccatctaaa aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc
     1621 tttttctaat atatgagcaa tcgtttctgt tccatccatc aagccggcca tcaaagctcc
     1681 taatgtcccg tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg
     1741 atctggtaag attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat
     1801 aattacgggt ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact
     1861 tgtagctcgg attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc
     1921 aaagtctaca cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta
     1981 gtgtccatca ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa
     2041 atcctgggca gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg
     2101 acgcaccaat gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt
     2161 actgaataac aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg
     2221 actacctaca tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat
     2281 aacttctaaa ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt
     2341 taattttgct tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc
     2401 taatgaacct gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg
     2461 ctgcataaaa ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt
     2521 ttccgcattc cagtttcttg cttcatattc gattggatca tagccaattg caataactag
     2581 atcacttcgt ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa
     2641 gaaatgcgct tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg
     2701 caatgctgtc ttttcaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg
     2761 catacctact aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat
     2821 atcatctgca gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattaccttg
     2881 gacaggtgca tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact
     2941 cttagccata cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc
     3001 tgcactgtat tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa
     3061 cttcaataga tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc
     3121 tgtcgctgtt accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac
     3181 tactcctggc tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc
     3241 gtgacgagtc acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat
     3301 ttttgcacct ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac
     3361 agatttcgaa ccttgtttca ttttttcagc cattgaacat cgtccttttt atgctttatt
     3421 tttaattatt agttagaaat aatcacaaat aagtgataca gtttctaaac ctgtttcata
     3481 caaaagtata gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga
     3541 atgacagttt ttacataaaa aaaactgtca ttcttaattg aaagtttcaa tttataaagt
     3601 ctacagatcc atagttctca tcaatttacg attgttgaag taaatcaaac caaatacgat
     3661 agaaggacct agtagaatac accattgggg ctttacgaaa aagaagagaa acaaacttgt
     3721 caatagtaaa aaatctagta aactcacata ccattttttc aacgtatagt agaacgctat
     3781 ccgtagaact tgatttggtt tcgacgctgg atttctcacc tgaaaatacc agcagttggt
     3841 gctaaaggaa actgataaac aagcaagtaa aacaaacaac ggaatcaacc attttccata
     3901 agaaaaacgt ataaaaaaga aacaatcaaa aatcattatc acactaacta tccaagcaaa
     3961 tagccaataa aaaaatccct ttagccaaaa ttggcgaaac atctttatga acaaacgtac
     4021 tggtgaaaaa ctattttctt ttgctaattc ttctgtcata cctatcatca cgatgcaacc
     4081 tggaccaata aaaagtaaag aaaaaataaa ggaaaagaca ttccttccat ctatcgacaa
     4141 aaggaacgca gctgctatat acaagatatt gacacaccaa aaacaaaggc tacttgccaa
     4201 tgattgatac aaccaataaa agaattccat ataaacattt ttgtcaaaac gctgctcttt
     4261 cataatcacc tcctattcct tcattcctga tgaagcgatc ccttctacaa aatatttttg
     4321 catcgctaaa aatataaaag caacaggaag aacagaaaca actgaggcag ccatgattaa
     4381 tgcgtactct gcatcaaata gcccaataaa cattttcaac cctaactgga ttgttttatt
     4441 ttccgtagaa gacaaataaa tgaatggccc catataatcg ttccatgtat taacgaatgt
     4501 gatgatcgtc aaactggcaa tcgccggttt tgtaagaggt aaaatgatct tgcgatagat
     4561 tccccactct gataagccat cgatccgcgc actctcacat aaagaatctg ggatactact
     4621 gtaatattgc ttcaacaaga acacaccaaa agcattgaac gcttgtaata gcaccaacga
     4681 ccacaacgta tctgttaaac ccacttttct catcataatg aactgcggga tcatataaga
     4741 ctgccaaggg acagcaatcg tgccgatata tgctaaaaat aaaatatcgc gccctttaaa
     4801 atttagttta gaaaacccgt aagcagcaaa actagaagta aacaactgaa tcaacgtgat
     4861 cacaacgctt aaaaaagctg tattcttaaa aaatgtcact aaaggaatac gagaccaaat
     4921 aaccagataa ttctctaaat gccaagtttt agggatccat tgaatcggaa tcgtgaacac
     4981 ttcattattt gctttgaatg aagcagaaac catccataaa aaaggaatga tcgtcaataa
     5041 agctaataca atcagcagaa tcatacccaa ccattgaata aagggcatgt tttttgaacg
     5101 aatttttgct ttttttcttt catttcgaac acctccaaag ttataatctt catttctttg
     5161 taatgattac aatccagaac aacggttatt atagctgatt catttccgtt cttcttattt
     5221 ttgtaagcgc ttatttaaca agttatgtaa atctctttat ttttttgatt taaaaatgaa
     5281 aaaatacaaa agcaagactg tcatacgtct cgcttttgta ttgaaatttc ttcgattaga
     5341 gatacttatt actgttcaaa agcttctttg aaacggtgaa tcaattcatt gactacaggt
     5401 gataaaatcc gattcttttt ccagactagc atgcagttcg tttcaatcat tgggtcaagc
     5461 ggaacgaatg tcactcgctt tgaatgatga cttacagcac cttctatgac taaagcagca
     5521 cctacctgat tctcaattag cgcaaataca ttgaaaatca aattgtacgt tcctgtgatc
     5581 agcaactgct ccttttttat gcctaaccag tcagcaaaca ttcgctgtat ttctggacgc
     5641 tttgagatca aaagaggtac attcgcaaag tcttcttttg ttatcacgtc tttttttgct
     5701 aaaacagaat tttctaaaat gagcaatccc catttttcct ttcttggtaa caaaatcgtt
     5761 tcgtatttct ctgtatctac tggatctagc aaaatcgcta aatctaacag gcttttttct
     5821 agtcactccg tgatatcatc acttgttccg ctgtggatat gaaattgaac gctgggataa
     5881 tcacggatca acttttctag taatagtgcc aatgtattcg aactatcagc ttccacacag
     5941 ccgatcgtaa atcggccgct gaataattgt ttttttctgt tctcgaattc ttgagtggtt
     6001 tgattcgtta aatctaaaat ttcttctgct cggctttttt agaaaataac ctgcttctgt
     6061 caagatcatt ttgcggtttt cgcgtacaaa cagttctgtt cctagttact cttctaactc
     6121 cttcagttgt cggcttaagg ttggttgtgt aagatgtaga acttctgcgg cttgagagat
     6181 tgttccttct tctgcaattg tccaaaaata ttttagtaag cgtaactcca tagcgatgct
     6241 ccttgatttt tttacttgat acattccatt ttatttctag ataacacacg atcatatttc
     6301 ttgatttttc cttgtaaacg ttccaaacat tcttgcattt cagcaatttt tttcgtcaaa
     6361 agttcacttt cttcaatcaa aagcttacgc cgctcaaata cagttgcttc tccttcgcta
     6421 gataggcgaa tatattcaat caaagactcg atcgataatc cggcatttcg catacagatc
     6481 gtaaagtcga cccattttag gtcttcttct gtataatcac ggattccgcc ttttgtccga
     6541 ttaactggtg gaatcaaccc gattcgttca taatagcgca atgtatcggc cgaaatgccc
     6601 ttttcttcac tgacttgttt gatattcatc tgatcactct ttctcttaat ttcttgccac
     6661 tttacttatg tattttattt attctacaac tttaagtgta ctccaagtca atactcattt
     6721 ctattaaaac taagctggat tatctatatc ggttgtttgt gtattatttc aaataaaaaa
     6781 gtcttgtttt tttattcttt tttggatata cttttaacaa tttagggaaa ttttaggaaa
     6841 aggggtataa aaatgacaac gtatcagcga aaggtattag tatcgacatc ggcgggaatt
     6901 gctttagaga atatggatat tatgttttta gctttttcat tatcttcaat gattacttct
     6961 tttcatatta gtggtacgca agcgggtttc atttcaacga tcactaattt ggggatgctc
     7021 attggaggta ttttttttgg catcatagct gataaatatg gacgtgtgaa agtattttct
     7081 caaacggtcc ttctgttttc gattgcttct ttattgatgt attttgcttc aaacatctac
     7141 ttggtttatc tctttcgctt tatcgcaggt gtaggagcgg gaggagaata tggggcgtgt
     7201 atgtccttga tttccgaaag tttttcaaaa aaacaaattg gacgagcttc ttcggttgct
     7261 ggtattggtg cgcaggtcgg tgcggctttg gctgcgattt tagctgctgt gatcattcca
     7321 acgtttggtt ggaagatgtt gtatgtcgta ggtgtactgc cggtgttgat ggtcttgttc
     7381 attcgtcgcg gcttgactga accaaaggaa ttcaaatctg tacaatctgc cgaaaagaaa
     7441 accaaactta gcagactgtt tgaatcaaaa catcttgctt ggcaaacatg tggccttagt
     7501 atcatggtaa ctgtccaaat tgccggctat tttgggttaa tgaattggct accttcaatt
     7561 atgcaaaatc gattagggct ttcggtttct gggtcttcat tgtggatggt cagtactatt
     7621 ttaggcatgt ctttggggat gatgactttt ggcataatca tggataaatt ggggcctaaa
     7681 ctagcctttt ctttgttttt aatttgttca tccttatctg tctatctact tgtgttagcc
     7741 catagccagt ggagtttggt agcagccgct gtgatagtcg gctactttat caatggcatg
     7801 tatggtggat atggcgcagt gattagtagt ttgtatccaa cagaaatccg cgcgacggcg
     7861 aataatttcg ttatgaacct tggacgagct gttggtggtt tttcttctat cgtcatcggt
     7921 ttcttgatgg atcattatag tttaactgcc gtaatcttgt ttttgagcgg tatttaccta
     7981 gtcagtctgc tggtcttgac aactcttact ggtgttacag cattaaggga aagcttgcag
     8041 cattgatagt tgagtctgta aatgacaaga aagaggtgtg gaaaaagctt gcatctgcgg
     8101 taataagacc agccaaatca aaaacatgag acactgtttc cgtttggctg gtcgtattac
     8161 ttgatgcagg gcagcttttt caactgctaa tttccttcta tctggcggcg actattcttt
     8221 gacgtccgtc atttcaaagt actttactaa taacttagca taacgaaaaa ctgtggatac
     8281 actcgtatat tgagcgtctg cataatcttc caaatttttc tcaggatgtt gatacatgaa
     8341 ttcaatcaaa tgataattat atgattttct gtagaaatga agataaataa tgtagattgg
     8401 gaagttctcc tgcttttcca tagaaaagcg ccgattattc tcattcttca aaatgacctt
     8461 atcaggatat tgttggaaca attcttctaa atctgttaga taattcaaga tagacttact
     8521 tgataccttc aattcgtttt gtagttcttc tattgaacac caacgttctg tttcaagcag
     8581 taaatgaatc attttaattt tcttttgaga aacttttgtt aaaaaagtct gcatattttc
     8641 tcctcctgta tctactttac tatactacat acaagaaaaa aacggataca aatatcatat
     8701 tgtcacaccg tcaatagtgc ataaacctgt t
//
LOCUS       DACDTO010000106         8649 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDTO010000106 DACDTO010000000
VERSION     DACDTO010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.106"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            90..557
                     /locus_tag="IWK70_002528"
     CDS             90..557
                     /locus_tag="IWK70_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9615023.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            564..3056
                     /locus_tag="IWK70_002529"
     CDS             564..3056
                     /locus_tag="IWK70_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9615024.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            3277..4200
                     /locus_tag="IWK70_002530"
     CDS             3277..4200
                     /locus_tag="IWK70_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9615025.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            4218..5459
                     /locus_tag="IWK70_002531"
     CDS             4218..5459
                     /locus_tag="IWK70_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9615026.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            5593..6939
                     /gene="gor"
                     /locus_tag="IWK70_002532"
     CDS             5593..6939
                     /gene="gor"
                     /locus_tag="IWK70_002532"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9615027.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(7874..>8649)
                     /gene="menA"
                     /locus_tag="IWK70_002533"
     CDS             complement(7874..>8649)
                     /gene="menA"
                     /locus_tag="IWK70_002533"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9615028.1"
                     /translation="YMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIG
                     VILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVN
                     TFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIE
                     NHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLD
                     QFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
BASE COUNT         3012 a         1442 c         1824 g         2371 t
ORIGIN      
        1 ggatttattt aacaaaaagc gttcttcttt ctttctatta tcaaattcgt tataattaag
       61 gtcaatatta gtcaaaggga atgaataaga tggcacatca gaatacctcg gatttaattg
      121 aatcatatat aaagaaaatt ctggaaaata gtgaaatgat cgagattcga aggattgaaa
      181 tggcagacct ctttaactgt gttccttcac aaatcaacta tgtgattaat acccgtttta
      241 ccatccaacg tggttatcta gtagaaagca aacgtggtgg tggtggttat atacgaattg
      301 caaaagtaag gatttcagat aaaaaacaat tacttgagca gataaatcag ttatttgatg
      361 atacaatcag tgagaaaaat gcttttgcta ttattcaaaa actttatgaa gaccaaataa
      421 ttacaaaaaa agaaggaaac ttgatgttga gtgcaattgc aaaaaacaca ttgaatttca
      481 atgaatatga agatcataca cgtgctcgga ttctccgagc atttctggaa cgtttaagtt
      541 atgaagatgg gaagtgagat tagatggaag aactttttac tgaaagagcc caagcagtat
      601 tagaaattgc acaagaagaa gcaaaacggt tgaaacatca atcagttagt tcagaacatg
      661 ttttactagc cttagtggtc gagcaaaatg gaattgctgg aaaagtatta cgtgaaatgg
      721 atttgaatga aacggaaatt tatgaagaaa ttgaacatct gattggttat ggtggtatcc
      781 gttcttaccc taaaggagtc tttcttccat attcaccaag aatgaaacaa gtttttgctt
      841 tagctagcag cgaagtgaag aaaactggtt ctcaaaaagt tggaacagaa catattttga
      901 tgagcttatt aaaagatgaa agtatcatgg ctactcgaat catgatcaac ttaggtatca
      961 gcctttcaaa agcccgtcaa gttctgaaac aaaaaatggg gctagctggt gattctgctg
     1021 gaaaaggaat gaatcgtcgt agaaatgtaa atgtacaaga taaacggcaa acaccgcaag
     1081 gaacacctac gctagattca ttagcacgtg atttgacgaa attagcaaaa gaaaacaagc
     1141 tggacccagt tgttggtcgt tcgagagaag tgaaacggct tatccagatt cttagccgac
     1201 ggacaaagaa taaccctgta ctggtgggtg aacctggtgt aggcaaaaca gccattgctg
     1261 aagggttagc tcaaaaaatc attttaggtg aagtgccaga agacatgcaa gaaaagcgcc
     1321 tgatgatgct tgatatggga tctttggttg ctggtacgaa atatcgcggt gaatttgaag
     1381 atcgaatgaa aaaggtcatc gacgaaatct ataatgatgg tcaagtaatc ttattcattg
     1441 atgaattgca taccttaatt ggtgcaggtg gagcagaagg agcaattgat gcttcgaata
     1501 tcttgaaacc ggcgttagct cgaggagaat tacaaacgat cggagcaacg accctagatg
     1561 aatatcaaaa atatatcgaa aaagattctg cattagagcg acgttttgct cgcattcaag
     1621 tagatgagcc aactcctgaa gaagcagaag tgattcttca aggattacgt acaagatatg
     1681 aagagcacca tggagtggag atcacagatg aagctgtacg agcagcagtc aatctttctg
     1741 ttcgttatat cacttctcgt caactacccg ataaagcaat tgatttgatc gatgaatcag
     1801 cagcgaaagt ccgtttggat caaaccgatc acttaacaaa atcaactgtg atcaaactag
     1861 aaatcgatga actagtacaa gaaaaggaag cagcgatcca aaaacaagac tttgaaaatg
     1921 ctgctcaatt acgcagacaa gaaaaagcac tacgtaaaaa gctgcaaaaa gtttctgcaa
     1981 ttgaagcaaa acaggagcaa ggttattctg atcgtgtaac agaagaagat gttgctacag
     2041 ttgtatcgga gtggactggt gtccctctac agcagttgga gaaaaaagaa agcgaacgct
     2101 tgttagaact ggaaggactt ctgcatgaac gtgtcgttgg tcaagatgag gctgtcaaag
     2161 cagtttcacg tgcgatccgc cgtgcacgaa gcggattgaa agatcctgat cgtccaattg
     2221 gttcattcat gttccttgga ccaactggtg tcggaaaaac agaattggcc aaagcacttt
     2281 cagaagtcat gtttggaagt gaagatgccc tgatccgtgt tgatatgtca gaatttatgg
     2341 aaaaatacag tacaagccga ttaatcgggt ctcctccagg atacgtaggg tatgatgaag
     2401 gtggacagtt gacagaaaaa attcgtcaaa aaccatattc agtgattctc ttagatgaag
     2461 tggagaaagc acatccagac gttttcaatt tacttctgca agtattggat gatggtcatt
     2521 taacagattc taaaggtaga aaagtggact ttagaaatac aatcatgatt atgacttcaa
     2581 atatcggagc gacacaaatt cgagaagaaa aaaatgtcgg attcaatgtc caagatgtta
     2641 ctaaagatca caaagccatg caaaaacgta tcttagaaga gttaaagaaa gctttccgac
     2701 ctgaattctt gaatcgaatc gatgagactg tcgtcttcca ttcattgagt aaagatgaaa
     2761 tccatacaat cgtccaaatc atgagcaaag cgattatcaa acgattgaaa gaacaagata
     2821 ttcaagtgaa aatcacacca tctgcaatcg atgtgatcgg gaaagctgga tttgatcctg
     2881 aatatggtgc acgtccaatc cgtcgcgcac ttcaaaaaga aattgaagat cgattgagcg
     2941 aggctttgct tggcggagaa attcgtttag gtgaccacgt aacagtaggt gcttcaaaag
     3001 gtaagatcac attgaatgtt cgcggaccta aaaaggaaac agtaggttcc ctatagttac
     3061 ttggcctgtg tagctgaatt tttataatag taagacttga ttaagaggtt gtgacaagat
     3121 ttttgtcaca atctcttttt atatttggac agaatatatc gaactttatt ttgatttttt
     3181 gtgaaataat tttcaaaaat ccgactttaa atagaagaag tattggaaag tttttagagc
     3241 tatgctacta ttatgaagtt gtaggaggag aaaattatga tcgaactcat aaccacagta
     3301 gaaactattg aacaagcaga acagttgctt gctgcaggtg cagatacact atatttcggt
     3361 gaagagcaat ttggattgcg tctgcctgct tcatttacac gagaagaaca gcgacaattg
     3421 gtagaattag cacacaagta tggtaaaaaa gcgaatattg ctgtaaatgg gatcattcat
     3481 cctgaaaaaa tgaaaaatgt acctgaatat ttggcttttt taaaagaaat aaacgtagat
     3541 gtgatcacag ttggtgatac aggtattatt tatacgatga aaaaaaatcc tgaattgcgt
     3601 ataccgtata tttatgatgc tgaaacatta gtaacgagcg cacgtcaaat caatttctgg
     3661 gcgaaaaaag gcgcagttgg agcagtactt gctcgtgaag ttccttttga agagatgaag
     3721 gcgatggaag aaaaactcga tattcctgta gaaacccttg tgtatggtgc tacatgcatc
     3781 catcaatcca aacgtccact gctacagaat tactataact atacgaaaca agacgaacaa
     3841 aaagatcgtg aaagaggtct ttttatctcg gaacctaaaa aagaagagac acattattct
     3901 atttatgaag atagtcatgg cacacatatt tttgccagca atgatctgaa tctctcgaat
     3961 gaattgaatg aactttatca gcatcattac tgtacatgga aattggacgg attatttact
     4021 ccaggcgaaa aattcgtaaa catcactcga ctattcgccg aagcaaaaaa acaaatttta
     4081 gaaggtagct gggatcaaga aaaagcggaa aaaatgacta gcgaaatcag taaattgcat
     4141 cctgaaaacc gtgaattaga tacaggattc ttttatattg atccttctgc tatcaaataa
     4201 ggagtatgat gaaagaaatg acagtaaaaa gaaaattgaa acgaccagaa atcctagcgc
     4261 cagcaggaac actggaaaag ttaaaaaccg cgatccatta tggagcagat gcagtatata
     4321 ttggcgggaa tgcatatggt ttaagaagcc gtgcaggtaa ttttacttat gaagaaatgg
     4381 ctgaaggagt tgcttttgca aaagaacatg atgcgaaggt atacgttgct gctaatatgg
     4441 tcacccatga aggaaatgaa gatggagcag gggaattttt ccgcagtttg cgtgatgtcg
     4501 gtatttctgc agtgattgtt tctgatccag ctttgatcga aatttgtgcg acagaagccc
     4561 caggtctgcc gatccattta tccactcaag cttcagcaac taattttgag acattggaat
     4621 tctggaaaga agaagggttg gaacgtgtcg ttttagctcg tgaagtttca atggaggaag
     4681 tggcagagat tcgtaaaaat acggataccg aaatcgaagc atttatccat ggtgccatgt
     4741 gtatctctta ttctggtcga tgcacattgt ccaaccatat gtcgatgcgg gatgcaaacc
     4801 gtggcggatg ttctcaatct tgtcgctgga agtatggatt gttcgatatg ccttttggtc
     4861 aagaacgaaa cagtttaact gatacaggcg aagtggaaga agaattctcg atgagtgcag
     4921 tggatatgtc aatgatcgaa catattccag aattgattga aaacggcgta gacagcttca
     4981 agatcgaagg aagaatgaaa tcgatccatt atgtatctac ggtgtcaaat gtctataaag
     5041 cggctgtaga cagttatatg gaagatcctg aaaactatgt ctgcaagcaa gaatggatcg
     5101 acgagttatg gaaagtagca caacgagaat tagctacagg tttttactac aatacaccaa
     5161 ctgaaaacga acaattattc ggtgaacgtc gtaaaatccc agtatataaa tttgtcggtg
     5221 aagtattatc ttatgatgag gcaacacaga ttgctacgat acgtcagaga aacttgtttt
     5281 ctgtaggaga tgaaatcgaa ttttatggtc caggattcag tcatttcagt caaaccgttg
     5341 aagttatgca taatgaagaa ggcgaatcca ttgaccgagc accaaatcca atgatgattt
     5401 taactatgcc agtcgcacag ccggtgaaac ctggagatat gatccgaaaa cgtaaataag
     5461 gaaaaagctg atatatccct gttttaagca tcttcgcata caaaattcaa gaaaagtgtt
     5521 acacttgttt tctgtagaaa aaggatcgtt aaataaaggt tttgattgat ctcttcaata
     5581 aggaggaaca aaatgcaaat atatgattac atcgtaattg gtggcggctc tggcggtatt
     5641 gcttcagcaa acagagcagg gatgcatggc gcaaaagttt tgctgattga agcgaatgaa
     5701 ttgggcggaa catgtgtcaa cgtcggttgt gtacctaaaa aagttatgtg gcaagcaagt
     5761 acgattttgg aaacaattga acgagatgcc tcaagttacg gtatccaagc agatttaaaa
     5821 aatgttgatt tcgccggtct agtagaaaga cgagaaaaat atattgattt tctacacggt
     5881 gcttatcaaa gaggattaga tagcaatcat gtcgaggcta taaaaggtta cgctactttt
     5941 atcgatagtc aaacgatcga agtaaacggt gaaacctatc gtgctcctaa aatcttgatc
     6001 gctactggag gccgtgcaag taaaatgacc attcctggtg gagaatacgc aattgattca
     6061 aatggatttt ttgaattaac agagttacct aagagagcaa tcgttttagg tgctggatac
     6121 attgcggctg aactttcagg tgtatttcgt ggtttaggta gtgaagtcat gtgggcatat
     6181 cgaaaagaac gaccacttag aacgtttgat aaaatgcttt cggacaattt gatccaaatg
     6241 taccaagaag ctggaataaa aacttacgca catcatattg ctaaagaaat tactaaaaac
     6301 aatgacgaat atacggtgct atttgaaaat ggtgaaacct taactggtga ttgtgtactt
     6361 tttgccggtg gacgtgtacc gaataccgaa aaattaggtc tggaaaatac aaatgtggaa
     6421 ttggacaaaa aagggtttat caaagtagat aaataccaaa atacaacgga tgaacatatt
     6481 tttgctgttg gtgatgtgat cggtaaatta gatctgactc ctgttgcaat cgcagctggt
     6541 agacgtttat cagaacgttt atttaatgga aagaaagatt cttatcttga ctataaactt
     6601 gttccgacag tcgtctttac gcatccacca attgcaacaa ttggtttgac agaagaagaa
     6661 gcattggaaa aatatggcga aaatgaattg aaagtttatc gttcacgctt taccccaatg
     6721 tactttgcat taaatgatta tcgccaaaaa tgcgaaatga aattgatctg tgtcggaaaa
     6781 gaagaaagaa tcgtcggctt acatgctatt ggtgttggtg tcgatgagat gctgcaaggt
     6841 tttgccgtgg ctatcaagat gggtgcaact aaagaagact ttgacaatac agtagccatc
     6901 catccaacag gagcggaaga attcgtaacg atgcgataat ttaatattaa cagttccaac
     6961 agctttttat tgaacaaacc atgtaaaacc agaaaatcag tatactgatt ttctggtttt
     7021 ttgtttttta gttatttatg taagattgct tcgataattc tcaaaacgaa ataagtaggg
     7081 aagttggaga ttttgattat cttcgtaact gacgaagagt aaatgaatcc atttggtatg
     7141 cactcttaag ttcaaaatat tggtatcgat gtttaaaaga aatagaaatg tatcgaagaa
     7201 cagatgttta aaaaagtgtg actttgtata aataaattag aagaagcaaa cgaggatgtt
     7261 aaaagaatac catatagtta ccaaaccaag aaagttctcg tcaagttcgc tggtacgaat
     7321 caaatcaagt gaaaaaaggg actgtgaaaa aaattgtgtc atagttagtc actttttccg
     7381 aacaaatagt gttcaatcag ctgtccttcg gtattagctc aaaaaacggc aaaatatgag
     7441 gaactatttt tccatttgtt tctctaatac tcaggacaaa acactgattt tacaacctct
     7501 gataaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
     7561 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     7621 tgataaacgg tgttcaaaag ccagtttctc ggtattaagc caaatttatg caaaaatgtg
     7681 gaaagccatt ttcacaaaat ttttcttaat actcgaaact aagcgacttt ttcacaacct
     7741 cctgataaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     7801 ataaggagct atttttattt ggctgttctt attacttgat gcagggcagt tttttcacaa
     7861 cctcttcatg ctgttaaatg gcttgcataa tgattgttag taatagcccc aatacataac
     7921 tggcattgaa taagaccata tttttgatgg aatagctgaa actgataggc tggggtaacg
     7981 tttgcttgaa ttgatccaaa tgcttcttca caattggcaa ggtcaagaag gtgaccaaga
     8041 tcggccattc atagataccg ctcaacaacc ccattaaaat cacgaaatag caagcataca
     8101 tcaatagctg gaataagact gttcccgcag gacgtccgat ataaaaaact agtgtatagc
     8161 gatgattttc aatatcacgg tcaagatccc gcaaattatt agccaacatg atattggcaa
     8221 tcgtaaacac caagggtaag gaagctaata cgattgctgc tatagcccaa agatttccta
     8281 ccaaatcaaa ttgtccagat gaaaagttga ctgtcagatc atagacatct gaaccgaatg
     8341 tattgacata aacgaccatg gcaaaaattc caaggcccat agtcacccca ctgaaaattt
     8401 cccctaaagg cattcgagat aaaggaatcg gtccgaatgt atagaatact ccaataaaac
     8461 aacagataaa tcccatcaaa agtaaaagcc aacccgtatt gatcgttaga ataacgccga
     8521 taacaaaaac aaatgcaatc atagctaaaa tcatgtttcg aatcaataca ggagatagat
     8581 ttctgcggcc aatgacattt tcttcatact tatatacttc agaatgagcc ttttcataat
     8641 ccatgtaat
//
LOCUS       DACDTO010000107         8494 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDTO010000107 DACDTO010000000
VERSION     DACDTO010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.107"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWK70_002534"
     CDS             complement(<1..173)
                     /locus_tag="IWK70_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9615029.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWK70_002535"
     CDS             complement(593..1714)
                     /locus_tag="IWK70_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9615030.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWK70_002536"
     CDS             2042..3451
                     /locus_tag="IWK70_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9615031.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWK70_002537"
     CDS             3448..4176
                     /locus_tag="IWK70_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9615032.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWK70_002538"
     CDS             complement(4304..5215)
                     /locus_tag="IWK70_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9615033.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWK70_002539"
     CDS             complement(5232..6239)
                     /locus_tag="IWK70_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9615034.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWK70_002540"
     CDS             complement(6236..8365)
                     /locus_tag="IWK70_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9615035.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWK70_002541"
     CDS             complement(8370..8477)
                     /locus_tag="IWK70_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615036.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DACDTO010000108         8414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDTO010000108 DACDTO010000000
VERSION     DACDTO010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.108"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..282)
                     /locus_tag="IWK70_002542"
     CDS             complement(<1..282)
                     /locus_tag="IWK70_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9615037.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQ"
     gene            complement(288..1394)
                     /locus_tag="IWK70_002543"
     CDS             complement(288..1394)
                     /locus_tag="IWK70_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9615038.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            complement(1394..2530)
                     /locus_tag="IWK70_002544"
     CDS             complement(1394..2530)
                     /locus_tag="IWK70_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9615039.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            complement(2562..4013)
                     /locus_tag="IWK70_002545"
     CDS             complement(2562..4013)
                     /locus_tag="IWK70_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9615040.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            complement(4057..4821)
                     /locus_tag="IWK70_002546"
     CDS             complement(4057..4821)
                     /locus_tag="IWK70_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9615041.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            complement(4849..5547)
                     /locus_tag="IWK70_002547"
     CDS             complement(4849..5547)
                     /locus_tag="IWK70_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9615042.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(5559..6338)
                     /locus_tag="IWK70_002548"
     CDS             complement(5559..6338)
                     /locus_tag="IWK70_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9615043.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            complement(6354..7298)
                     /locus_tag="IWK70_002549"
     CDS             complement(6354..7298)
                     /locus_tag="IWK70_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9615044.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(7344..8126)
                     /locus_tag="IWK70_002550"
     CDS             complement(7344..8126)
                     /locus_tag="IWK70_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615045.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKEV
                     TSADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATA
                     TYDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
BASE COUNT         2386 a         1751 c         1257 g         3020 t
ORIGIN      
        1 ttgattaatt atttgatttt gactataatc atatgttttc aaaaaatctg aaaaagtctt
       61 ttcataatac ttcagcatta cctcatgttt ttttaataaa tgttccttct gttttttact
      121 ttcattatat ccattacgat tcatgacttg aatatcaatt ttagcttgta acgtggcaaa
      181 tgcttccttc caagatgtta tctttgcaat tcgtcttgtt gcattcaatt cttcggatat
      241 gcgatgtata attcttttta tacttgaatg tttctgtgtc atattcttta acctctttgt
      301 attaattcaa ataactcata atattttgat tctaagattt tgtatgctgt ttgaatatta
      361 taatctgcct ttattaaaat cgaacgttct gaatctcgat gagagatatc tacatttgca
      421 atctctctcg cccattcttt tgccgattga tttaaactta tgaaattggt cttatcactg
      481 aaagcaactt ctgtaggaac tttctccgaa aagaaacacg gtaaatctgc aaattgagct
      541 tcaataccca ctaacggaac cccttcaaat aatgatggca acattaatac gtcaaatgct
      601 tgataaaatt tttctatatc atttctattt cctaaaaatt taatatcatc atctatgcca
      661 cttttctttg cataattttt tatttcttct tccatatcac cagttccaat aattaataat
      721 acggaattat tcttaatagc gtgaaattct ttaaaaattt caatcaaaaa acgatgattt
      781 ttagaatatg aaattcttcc aatatgtccc attacaaacg tctgacttga aattcctaat
      841 ttcattcgta catcatttct aatactttca tcataataaa attttttagc atccactgca
      901 tttggaatca aagtgaattt atcatttcga aacaaaaaat cacctgctac ttttccacat
      961 gaaaattcat gtgtcaaaac agacgttaac ttacatcgat aatattgctt taaaagatat
     1021 ttaaaatctc tatctatcga tgtattatgg ctatgtgcta tccgtatcgg aatcccagct
     1081 ttttttgcag cttttaaagg taaataactc atcgcatcaa tatgagaatg aacaatctca
     1141 tattctggat gatccttaaa aaattgttcc atccatttaa aatatttagg ataattttgt
     1201 ggataaagtc tcggtgcata atagaccttt cctccaagac ttattatttc ctcatcataa
     1261 tcacttttat atggacgatg tgtcaaaaaa tcaaactgaa ttttagttcg atctatattc
     1321 ctataatagt tcattagcat cgtctccaac cctgcacgat gcatatcatt cacacactgt
     1381 aatattcgaa tcattatata tttcccctat acaaatttaa tgtttcttgt acgattttat
     1441 cccaattata tttactacaa ataaactcag tagcttgatt cttcaaaact ttaaccattt
     1501 ccgattgatt gcatgcctct tctaatctta tctttaaatc acttacatcc gatttcttaa
     1561 aaatcatagc cttgtcttct actacttccg tacactctga gatatttgaa actatacaac
     1621 agttcccata actcattgct tctaacaaac ttaatggcat tccttctaaa tcacttggaa
     1681 gtgtatagat atacgcatta ctatataatt cttctaattc ctgtccttgt acaaatccag
     1741 taaatataat tcgctcatca ccttttgcta attcctttag ttcatttgca aattcatctg
     1801 tatcagagct tcctccggct attataagtt ttttatctgt ctgaacatct ttaaatgcct
     1861 caattagata ccgaattcct ttttcgggaa ccaatcgccc caaaaacaaa atataagaat
     1921 cttttgttaa tccatacttt tctgtaatca atcctgctga ttttatttca atttttttaa
     1981 cgccatttgg tataaacctt gtttttcttc catatatatc ttcaaaataa ttttggacgc
     2041 tttcactaag tacaattatt tcatctgcaa attttacagc acacttttcc cctaacatga
     2101 tgtatgtact agcaagcttg ttccattttt ctctttgatg atctagacca tgaattgttg
     2161 caacacatct tttcccaaaa agttttggca accacaacat agcacatggt ccttctgcat
     2221 gaaaatgaac aacatcatat tttccgaatg ctgctctaat tccaccaaat actgaagaac
     2281 tcattgctgc aattcctttt atatttaatg taaaaatagt ctttaatttt acacctttat
     2341 attctttatt tttatgctgg tcaaattcct ttccgctaac atgatgacca cttcgattat
     2401 agcaagtcac ttcatgccca agtgcaacca ttcgcgttgc caattcttct accactatct
     2461 caattccacc ttctcttgat ggaattcttt tatgacctag catacagatt tttaattttt
     2521 ttcttgccat cacgttttac tccttctata gtatctttat cttacatgga accgtttcta
     2581 tgaagcacag ttttgactgt cctaaacaaa attctaaaat ctaatcccat actccaattc
     2641 ctgatatatt ctgcatcaag ttttacaacc tcttcgaagt ctgtgatatc acttctacca
     2701 ctcacctgcc acattccagt aatcccaggt ttcatcgcaa ttctcgcacg atgatgaaaa
     2761 tcatacttct catattctga cttcaagcaa ggtctaaacc ccaccaggct catttttccc
     2821 attaggacat tataaaattg tggaaactca tccaatgatg tagatcgaat aaattgtcca
     2881 attcctgtct tctttgttcc atcaggaagt attttattgc caatgattct tgggtcaaaa
     2941 tccatcttaa acatgagatt attactcatc ttattttctt tcatcaactc tgctttgcgt
     3001 tcttctgcat ccatatacat actacggaac ttatacatct taaatgtctt accattttga
     3061 ccaatacgtt cttgtgcaaa gaaaatcggc cctggtgatt tgatgtaaat aactggtgct
     3121 acgaagataa aaataacacc tgttgcaata catccaataa ttccagcaag aatgtcaatc
     3181 gttctcttta taaatgcttg cctcatcgtc ataacattaa tactagatgt aataacagta
     3241 taatcaccaa ctcggccaat aaattgtttt ccacgtgtac tatcagacac ttttgtaaga
     3301 ttcatatgta cagcaagtcc catttccgaa catctggcga ttaattcttt gggatatgga
     3361 acactttcgt caacattgat gaatacttca tctacccaat tctgtaaaag atattccgct
     3421 gcattatcag catttgcaac tactgggaca cctccgatgg tagttccata catatctctg
     3481 tctactacaa tcacaccact aagtttatac atgccataat tcttctcttt aagattacga
     3541 actacatttt ttgcaatctt ttgagatgtt acaataacta acgatacatc ttcaccgttt
     3601 ttcatgcggt gatgcaacaa ccctttccaa agtatacgaa ttatatatgt taataatcca
     3661 tacaccaccc ccattataaa taaagaaact ctggaataat catttccatc ctgcattgta
     3721 ataaggtata aactgcacac aagtaacagc ataaaagatt gctttataga agctacagcc
     3781 tctttataat atcctctttt taaaacattt ttatatgcct caaataaaaa tataatcgta
     3841 agattctcaa gttctataaa aattgccatg tggcgataaa taggaatcac atacggattc
     3901 cagtcaccct gtcgcattac atatgatata taaaaagcaa gctggacaca aaataagtct
     3961 aataaaataa aatcaatatg tttagaccaa ccacttgagt tttttctata catctctcac
     4021 tctctcctca tttccttttt atctacgaat aaagaactaa aataaaccaa acttcttctt
     4081 ttttacctct tcatagattg gataattgat ctcatccccg ttgatcagat cctttgcgac
     4141 ttgtttcatc tgtactactt tttccttacc aaactccttg gcaatctgct catagcactc
     4201 ttttaagtaa aaggtgcgct tagagacccc gtgtgcatca gaagcagcca tctgcacgag
     4261 attatgtttg accattgttt tggctgtttt ctggatatct ttgccaaata caccaacata
     4321 acttggcgca gtaagttggg ccaaacatcc catatctagg aacggaagca aatgatttgg
     4381 gtcttctcga aaatgcgcgt tgcgttctgg atggacgatc actggtatct ttcctagttc
     4441 taacaactca aaaaaaagcc gttctgcata taacggcact tccagtgtcg gaaattcgat
     4501 cagaagatat gtatcatcta aatctgtaaa taaaatttca tctcggtgga tgtcttcgat
     4561 gagcgttccg gtaatacgta cttcttgtcc ttccaataac gtcaaaggca actgtctttt
     4621 ctctaattcg gcttgtaacg aagcgactag agaaatgact tgtgattttt cattgctgta
     4681 ttttccatta ttgtggtgtg gtgtgcataa aatatgcgta atgccttgct gaatcgcttt
     4741 ttcagccatt gcaatacttg cttctaagtt ttctgccccg tcatcgattc ctggtaggat
     4801 atggcaatgt agatcgatca tttctcttgt ctcctctctt cttattcctt aatttccgta
     4861 gtaataacca gcatctttgc tgtgttctgc tccgttgtat acgacaccta agactcgtgc
     4921 ttgtaccata tctaacagtt cttttgcttt agtcaatgat tcttttcgtg acgtattttc
     4981 ccgtacgacc aataaggtgc catccgtttt tgaagacatg atctgtgcat ctgttacggc
     5041 aacgactggc ggcatgtcga agatcactac atcaaatagt tgtcttgctt ctgctaaaat
     5101 ctgatccatt cttggcgaac ttagtagttc tgatggattt ggtggtttag gtccacttgg
     5161 cagaatcgac aaattgtcga ctggtgttcg ttggatctca tctgctacac ttcctgatga
     5221 actaagaaca gtacttaatc cgcttgcgtt gttcaacttg aacgttttat agatgacggg
     5281 cttacgtaag tctgcatcga ctagcagcac ccgttgacca gatttggcaa aaacaacggc
     5341 aatgtttgct gcagtggtcg atttcccttc tccaggtcct gatgaagtca caacaatcgt
     5401 tttgatttgc tgaccagctg cggaggcaaa ctggatattc gtccgaatcg tacgatattg
     5461 ttcagcgatt ggtgaagagg gattggtgag tgtgaccaaa ctgactgcat gagtttgggc
     5521 tgctttttgt ttctgtgttc gtgccatttt ctttcctcct agactcttga gcggcttctg
     5581 cgtgatggcg ctgttttatc gtttgtagct tttggttgtg tctgagctgg tcctttttta
     5641 cggataccag cattcaattc tttcgctgac atttctggaa ctgttcctaa gattgggaat
     5701 cctaatgtat ctgtcacata tttttcatct ttcactgtcc gatcgagtaa ttccaataag
     5761 aatgccagac ctacacctac catcattcct aacattaagc cagtcattaa attcaattta
     5821 ttatttggcg aaacaggtgt cgtattggca actgcacctg aaatgatcga gattttatca
     5881 acattcatca catcttttgc gttctcttgg aaaacttgtg aagttgtgtt agcgatctgt
     5941 tgagcagcca ctgcattcgt acttattgcc tgaatcgaga acatttgtga attttctgcc
     6001 tgattaactg agatggcatc tttgatttgt tcagcagtca ttttcacatt gtcttctgtt
     6061 tctaaacgat ctttgacttc attgagcact aaatcactga cgatcatatc tttatacgta
     6121 ttgatcattt gcaggttcgt attgacatca ttgacatttg cagtgtctga ttgtggcagt
     6181 gtcacgatca attgtgcttg tgaactgtat tttggtgtca agatgaaaaa tgttacgatt
     6241 cctgagatgg ccaagccagc aaacattgag atgatgatta ttaccaagtg tttgcgtaat
     6301 gtttcaaaaa tttctttcaa gctgattgtc tcttccattt tttctccact ttcctattta
     6361 ttctctagat tcatttgatc ttttaattct tttttcacac gatcaagttc ttgatcactg
     6421 actttttgat aggagatatt atcttgcatg aagccttctc cttgcagctg atcttgctta
     6481 atcgtaccaa atgcctcccg ataatctaaa gcaatttttt tcatttcatc aaagttcatg
     6541 tctgttttca tattcgactc tagagcattc aaaacggatt gatagttgct gacaccgttt
     6601 agactcaata cttttttcgc tacgccttct acgatctttc tttggcgccc ttgtctaccg
     6661 taatcaccat ttgggtcttc ataacgcata cgagaataag ataatgcctg atccccgttc
     6721 aatgtaattt taccgattgg aaagtcatag ccatcttgtg agaaagtgag gtcattattg
     6781 acttcgatac caccaactgc atcaacgagt tcctttagcc ctttcatatt gatagagaca
     6841 tagtgatcga ttgggatgtc tacatatttt tgtacggtat ccatcgccat ggcagctccg
     6901 ccgaatgcgt aagcgtggtt gatcttgtct tttgtgtcac gacctacgat gtcgacatac
     6961 gtatcacgag gaatgctgac gatcgttgtc tgtttgtctt caggactgac tgtggcaacc
     7021 atcatcgtat ctgatcttcc ttgatcgacc cgacctaaat ctccagtatc aatccccatc
     7081 aatagaacag agaaagattc ttgtttgtcc agatcaactg gtttcgttcg ttctccttgg
     7141 gttcgttcca ctgattcgta agttttctca atagaatgtg agagatccag atataatttt
     7201 gctcctactc ctacaattgc taatactaat acgataagaa ctgctaaaat aatcagaaat
     7261 atttttttcc ctcttgattt tcttcttcgt cttgccatag tttcctccgt ttgctaacct
     7321 tgcttgaatc aacgttttaa ctgtcaatag cttatattgc tatagcccat ttgctcagca
     7381 acagtttgtg cttttgttgc gtatgctgcg cttatttttt cattccggat ctgctctatt
     7441 tgactgagca ccgtcaaata ttgatcgtaa gtagcggtgg cagtcacttg accatccttt
     7501 aacatcgtat cgaataactg atctagttgc gtgacttgcg cgatctgaat atcagcgaat
     7561 cctagatatt gcataacaac cgttttccaa ggactatctt taaaaaagct catattttca
     7621 cgtacacgag cgacatctgc acttgtcacc tccttttctt tgatgatgac atcgtctaca
     7681 gcttgctgcc agtttgatac atttttagta aataaatcgt tgacagctgt ctggatcttc
     7741 agttttgatt ctgcactatc taactgtgtc acgattgctt tcttctcatc ttggattgca
     7801 acagcttctt taggcaattc tgattctttg atgtcgtaat catctgctga aagtttgaca
     7861 gaggatagct tatttgacaa acttgtgatt gtttcttctg ttatttcagg gtttagaaaa
     7921 attttttctt ttgtactata cagcttgtct acttcttttg ataaagaagt caaggtgtct
     7981 ttttgattgg tgatggcaga ttctgcataa ttgatccgtt gctgatgttg ccgctgatta
     8041 ttgatatggt atccaaacaa acagatcgca atagcacctg cacctaccgc cacagcagtc
     8101 aatgacttga cgaatggctt ttccatagat tcactcctaa gtattgatga tagataaatt
     8161 aagatataat tcacttacat aaaaatgaca gctttttatt taaaaaaaca taattaaact
     8221 aatatattca cagaaatatt tgtaaacaag ttttccctat ataatcatac cacaaactgt
     8281 tttcttgact agggcaattt acgatttaaa agtaaataaa acattaataa gataaaaatt
     8341 aatcgatttc atttttattt ccataatttt cataggtgtt tttaaaagtt acacaaaaaa
     8401 tagaataaca aaaa
//
LOCUS       DACDTO010000109         8071 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDTO010000109 DACDTO010000000
VERSION     DACDTO010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.109"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IWK70_002551"
     CDS             complement(37..216)
                     /locus_tag="IWK70_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615046.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IWK70_002552"
     CDS             complement(597..1304)
                     /locus_tag="IWK70_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615047.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IWK70_002553"
     CDS             complement(1297..1734)
                     /locus_tag="IWK70_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615048.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IWK70_002554"
     CDS             complement(1749..2000)
                     /locus_tag="IWK70_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615049.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IWK70_002555"
     CDS             complement(2000..2206)
                     /locus_tag="IWK70_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9615050.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IWK70_002556"
     CDS             complement(2643..2903)
                     /locus_tag="IWK70_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615051.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IWK70_002557"
     CDS             2978..3181
                     /locus_tag="IWK70_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9615052.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWK70_002558"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWK70_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9615053.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IWK70_002559"
     CDS             complement(3810..4709)
                     /locus_tag="IWK70_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9615054.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IWK70_002560"
     CDS             complement(4722..5315)
                     /locus_tag="IWK70_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9615055.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWK70_002561"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWK70_002561"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9615056.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..>8071)
                     /locus_tag="IWK70_002562"
     CDS             complement(7771..>8071)
                     /locus_tag="IWK70_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9615057.1"
                     /translation="INLKDICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSY
                     KAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
BASE COUNT         2054 a         1654 c         1382 g         2981 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gcgatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat a
//
LOCUS       DACDTO010000110         7941 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.110, whole genome shotgun sequence.
ACCESSION   DACDTO010000110 DACDTO010000000
VERSION     DACDTO010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.110"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..497
                     /locus_tag="IWK70_002563"
     CDS             <1..497
                     /locus_tag="IWK70_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615058.1"
                     /translation="SYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTLI
                     APILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLCL
                     VINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYFY
                     RNKL"
     gene            complement(623..1300)
                     /gene="nth"
                     /locus_tag="IWK70_002564"
     CDS             complement(623..1300)
                     /gene="nth"
                     /locus_tag="IWK70_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9615059.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1307..2005)
                     /locus_tag="IWK70_002565"
     CDS             complement(1307..2005)
                     /locus_tag="IWK70_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9615060.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2273..3457
                     /locus_tag="IWK70_002566"
     CDS             2273..3457
                     /locus_tag="IWK70_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9615061.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3463..3906)
                     /locus_tag="IWK70_002567"
     CDS             complement(3463..3906)
                     /locus_tag="IWK70_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9615062.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4132..4419)
                     /locus_tag="IWK70_002568"
     CDS             complement(4132..4419)
                     /locus_tag="IWK70_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615063.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4590..5942)
                     /locus_tag="IWK70_002569"
     CDS             complement(4590..5942)
                     /locus_tag="IWK70_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9615064.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6215..6661)
                     /locus_tag="IWK70_002570"
     CDS             complement(6215..6661)
                     /locus_tag="IWK70_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9615065.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6897..7823)
                     /gene="cysK"
                     /locus_tag="IWK70_002571"
     CDS             complement(6897..7823)
                     /gene="cysK"
                     /locus_tag="IWK70_002571"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9615066.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2157 a         1606 c         1285 g         2893 t
ORIGIN      
        1 tgagttatct gatagtatgc ttaattatca taatttgtct gcaccatctt tttttatcga
       61 aaacaatacc tcgttattcc aagaataaaa aagctgaaaa attttgttta ttattaaata
      121 aatttacttt aattgctcct atccttgcat ttattatttt ttctgtactt ttaagcacta
      181 ctcttaaagg aaaatttatg gaaagaagct ctcatgcaat gattctcact tttttatggt
      241 tgttgttcac cagaatctat atttttctga tgagcctgaa accgcccaaa tccattagtt
      301 tatgtttagt tataaatggt atttttttat tatcacttat catttttatt acacctttag
      361 atagatacgt cacttattta tataatcctt tggaatactg gacttatttt ataggtatat
      421 tggaaggcat tattttctac atcgggtatt ttcccaataa aaataacaac ttttattttt
      481 atcgcaataa attgtaataa gttaccatcc tagctactga gtatgtacat ttactttttc
      541 atgcaaaaaa gataaacgaa acagcttttc tccataataa caagataatt catacattta
      601 tttgcaatta aagaaaccac gtctacaggc gtggtttctt ttttagcgcc ttttctttta
      661 gacgcatccg atttttccca tcttgacata ttgatagcaa aggacatacc tcgcacttgg
      721 gatttcttgc cgtgcaatga tatctaccaa aaaaaatcag tgtatgatgt gtctttaccc
      781 ataattcttg tggtactttc cgcatcagtg tttcttctac ttccatgacc gtagcatcta
      841 gtttgcagat cctcaggcgt tttgagacac gttccacgtg ggtatctact gcgattgctg
      901 gtatcccgaa tgcatccccc aatacgacat ttgctgtctt tctgcctaca ccaggaagag
      961 acatcaattc ttctcttgac gttggcactt gcccatcgaa acgttcaatc agctgctgtg
     1021 cacacgcttt gatgtttttt gctttatttc gatacaaacc aatcgttttt attttaagaa
     1081 tgatttcatc gattgaagct tctgctaacg catcgggtgt cggaaaagag gcaaacaaat
     1141 ctggcgttgc tttattcact gatacatctg tcgcctgggc acttaagatc actgcaatca
     1201 ataactcaaa tggattattg tgcttgagtt ctccatgtgc ttcgggaaac attccataca
     1261 tcgtttctag agcttccatt gttttttgtt tgcttaacat attccgctac tcctccaacc
     1321 aatttttcag tgagactttc ggcaattctt cttgttgagc tgactgttct acttctcttt
     1381 gaagaagctg ctgttttctt cgtttttggt cttcttccac ttgttgtttt gtacgtagat
     1441 tttttctttc ccacgatagc aaaatgcggt caacgtaatt aaaactataa gcctgattta
     1501 atacggcttc ccgcagtgct agcttcaaaa tttccggctg ataatggtct tcttctagcc
     1561 attgtccgat ccgctggaac tcgatcgaag aaagcgggcg tccaaattct tgttcaaata
     1621 attgatacac tgactggatt tctttttctt gtgttttttc ttcgtatttt ttttcttgtg
     1681 tttttaaata ctcacctaat agatcataga tggggtaaag attatatcga tctgcttttt
     1741 taccgttgac aatcgtcgtt tctatcttga taaatccatt tgttacgaga cgatccagta
     1801 tttgaaaaat ctgatcttgt ttgattccca tatccatagc aatcatctgc aaatcaggga
     1861 aagtatctcc ttcaagctgt gccatatgca actgtagaac aaataaaaat tcttcagatt
     1921 gtagaccgat cctatgataa tttttaagta aaagattaga tatgactgtt tgtccagcgc
     1981 ttaagtaatt ttctaaattc aacatgattt ctcctccgct caatcacagt atacgtaaaa
     2041 aaaacatggg aagcaagaac aggtttcttt tctatttact tcactgatcg gtcagattta
     2101 caatgtcgtc tacttgattt ttgtttgttc atgtttttga tgcgtatctt cacttttcgg
     2161 aataaataaa ttaagtagat ttcgctgttc cttagaatgt tgacaaggaa acaaaattac
     2221 tatacactat ttttgtttcc aagttaacat tattgcataa aggagttttt acatgtacag
     2281 tttcacaaat aaacattcta aagtttttgg atacgtctct gtcttattca ctggattagg
     2341 atttactatc gtcagtcctg tacttccgtt tttaacgctg ccttattctc actccataca
     2401 tcagcaagct ttctatatca ctttgctcat gtctgtttat gctctagctg ccttcttatc
     2461 tgcccccatt ctcggtagct taagtgatca ttttgggcgt catccgatcc ttattatcag
     2521 tttattagga tcaagtatcg gatatttgct ttttggttta ggaaattcga tatggatgtt
     2581 gttccttggt cgtatcattg aaggattgac ggcaggagaa atctccacct tatatgctta
     2641 ctttgccgac atcacggaac ctaatgaaag aaccaaagtt tttggatgga tgggtgcttt
     2701 agtcggtata ggaacaacgc tgggtcctat tatagggggt ctattagctg agttggggaa
     2761 ttctgttcct atatttattg gagcactctt tactttctta aatgccgttt atggctacac
     2821 atttatgcta gaaagccttc caataaaaaa acgttcagta gatttaagtt tctcccatgt
     2881 aagacctttt caccaattac aacaattatt caagatttca tccgttattc ctttattgac
     2941 agcaggtttt gctgtttggt tggcagctgg ctctcttcaa tctatctttt ctcaattttc
     3001 gattgacgcc tttcagtgga aggctgggtt ggttggttta agcttctctt tgattggaat
     3061 cttggatact gtctcgcaat tgttcattat gcctcgattg ctcaaaaaat tttcagaaca
     3121 gcagatcaca agaataggga tgttcagtga aatcctcgcc tatctattca tcacattatc
     3181 tggaattctt ctactgccga ttttgttttt atttgggatt atctgttatg gtttcggtga
     3241 ttctattttt actcctgtat tcaatggcca actatccaat tctgtctcag ataaccagca
     3301 aggtcttgta atgggcggta cccaaagtat acaatctcta tcgcgcgtca tcggcccgct
     3361 aatcgcagga cagctttatg ctgcagcacc atttcttccg acggtattcg gatttttgct
     3421 tatgattttt gcctattatc actacaataa aaaatagtag cttcatttgc tacttcgtat
     3481 aaattctctg tatcgaccga taaaacgaaa tactgtgtca aattcttctt ttgacatatt
     3541 ttcaaataca gtagcatctc gctgtaccca tttttgatgg agttgctcgt gtctattcct
     3601 tattttttgt ccgattggcg tcaattgaaa atagatttct tttttattct cttcgctttg
     3661 ataagtgttt atgatccctc tagctatcag tttttttgtt agcttactga tggctcctct
     3721 agtcatgtaa ctagcattcg ctaattttgt cacgttagga tcagggattt tcccgatatg
     3781 ttcaatcact tctagttcat ttagagtaaa tcctgcaaat tctttttcca tttctggttt
     3841 attacgatga ataatctcat tagctaaatc cagatattct tctattagca gttgtctttt
     3901 atccatttct acaccttctt tttctttcat tatattgttt ttttgtttcc tattcaacaa
     3961 agataaaaat attttgtagg aaaacccaat ctgaaaccaa gaaaaaaagg ggttgacaaa
     4021 gcttgtcata accccttttt ctgattaaaa gatagtaaag agacagtcat cggtaattgt
     4081 gatgattttt tgtcatcact taaggatgag cggccttttc aaaaactgtc tttaaagatc
     4141 atcatcagtt tctagcacat atggctgacc ttcatatgtg tactctgtat tcaatgtgcc
     4201 gtcattttca tctgagtaga tgcgtctact gaaaggaaaa cgatgagaaa cattatcttt
     4261 ggcgtcattt gctggcatcg tgtaatattc ctcttgcgtt tcattcaact gagagagtac
     4321 ttgatctgcc aactcatcaa tattgatcgt taatttcttc gaagcacttt tataatcttc
     4381 taaaaatgca tcttgttgga caaaatattc tttattcatc ttctttctca cctttctttt
     4441 caaaatcaaa taaagctgct tgttctttta tgtaacagct ttatctttgc tgcttattgt
     4501 gacaaaaaat tcgaagaaag acaactaatt gtacttttca caaaaaaaga cagagggtcg
     4561 tgacaaaatt gtgtcacaac cctttttctt tattgtgcag tttcgatttc gcctagcgct
     4621 gcttgtgcaa gtaggttcaa gtaattccat gggcggtcaa aatgtggttg gaagaagaaa
     4681 tctgaaagtg cgagatcttc tacagtcatt ttattttgaa cagctaaaga catggtattt
     4741 gctgattgtg taatgtcgta tttggacatg aactgagcac ctacgatacg attcgttcct
     4801 ttttcataga ctagctccat cattactttt tcagtagttg gcatgaactc tggacgataa
     4861 ttatcttcaa atacagtcgc ttggacttct agatcgttca aagttgcgct ttctttggtc
     4921 acacctgtcg aaccgatcgt ccagccaaat aggtaaagtc ctgaagttcc ttgagtacca
     4981 cgatacgcca ttttatgatc tgtcaagtta tttcccacaa gcaaaccttg acgaacggca
     5041 tttgttgcca aaggaatata gttttgcgtg ccacttggat tgtagttgac tactgcagag
     5101 tctccagctg caaaaatgtc tggattactt gtctgcatgt attcattcac tttgattgcc
     5161 ccgtttggca gtgtatctac ttttccttcg atcagttttg tatttggacg gaagcccaca
     5221 cataaaatga ccatatctgt atcaaatgta tctgttggtg tagctacttg tttcactttt
     5281 ccttcttcat ctgtgataaa ttcagtaaca ttttcgccta gcgcaagagt aacaccgcgg
     5341 tctgtcaatt ctttttctaa gatatctgtg aatggtttat ctagatattt attcaagatg
     5401 cgatctaatc catcaatcaa tgtcacttgt tttccagatt cgacaaaagc ttctactaat
     5461 tcaataccga tatatccacc accaacaatt actacacgtt ttgcttcttt cgcttgtgca
     5521 ataatttcat ttgcttgatt gtagtttttg cataggagta catttttcgc atcgattcca
     5581 ctaatcggtg ggataatagg ccatgaacca gttgtcatca ctaatttatc ataagaaacc
     5641 gtttctgttt ctcctgtttt cagatctttt gccgttactg ttttattttc tgtatcgacg
     5701 tctgttacat cgtgttccat tttaactgtt gctcctaatg aagataattc ttcaggattt
     5761 gagtagaaca agcctgctgg gtcctttact actccgccga cataaagtgc gataccgcaa
     5821 gataagaaag aaatattgtc atttctttca tatactgtca cttctgcttc tggatgattt
     5881 tttaaaatac tttttacagc tgcagtacct gcatgagtac agccaattac tactactttc
     5941 atttatggtc cctccgatta acattttgtc cttcgacaaa actactataa cagaaataaa
     6001 acggatacaa cagtttaatg cttgtgaatt cacaaaggtt tgtttgtagt ttcattcctt
     6061 ttcaagcata agaacccaat atctacaaga ttcttatttt aataatattc acttttatat
     6121 ctaagattgt gaataaaaga caaaaaaata aaaaagctag cgtttttttt acacagctag
     6181 ctttttttag gtataacact tgtagaaact gtttttacat ttgcgccttt tttgctgact
     6241 ctttacaatc cggacagagt ccatacaatt ccaaccgatg ttcattaatc tcaaaatcag
     6301 tcagtctgct tgcagccatt tctatatctt caagccctgg gtaatgaaaa tcaacaatct
     6361 taccgcagtt ttcacaaatt acatgataat gacgctttga actgaaatca aatcggctag
     6421 aagcatcgcc gtacttcatt tcttgaacaa atccaatcga agtaaataag cgaaggttat
     6481 tgtaaacagt cgcaacactc atatttggaa accgatgttc aagtgcttta taaatctcat
     6541 cagccgttgg atgggaatga tgttcaatca aatattctaa aaccgcgtac cgttgaggtg
     6601 tgattcgaat gttctctttt tttaactttt cgataacctc ttcgactaac ttattttcca
     6661 tgtaaacacc ccacgttgtt attctcgtct tatcatacta tttttacatt ttaaaatcca
     6721 cttaacatga aaagaaaatt caattttatt gtattttaat gaaaaaagac gtaaaacagt
     6781 cgcgttcact attctacgtc tctattcaaa taatttttct catcataaat ttttgtgtgt
     6841 tcttttcatc tttaaaagtt ttttacataa acctatctgg agaaaaacca agtgccttat
     6901 atctcttggt aaagggctgt agataaataa cgttcaccat tgtcgggaac cacagctaat
     6961 accttcttgc cttttcctaa ttcttttgcg acttttagcg cggcagcaat tgcagcgcca
     7021 gatgagatgc ctactaaaat cccttctttt cgcccaactt ctctagctgt ttccatcgct
     7081 tcgtctccac taatagaaag gactttatca tacacagaag tatccagtgt tttaggaaca
     7141 aaacctgtac cgattccttg gattttgtga ggtcccgctt ctttcccttc taaaatagca
     7201 gattctgctg gttctacccc gattaattcg attttcggat acacacgttt taattctcgg
     7261 ccagcgccag tgatcgttcc tcctgtaccg atacctgcaa caaacgcgtc caagccattt
     7321 actccaaatg cttgatgtat ctcaggacca gtcgtcttct catgcacaag aggatttgct
     7381 tcattttcaa attgtaatgg taaaaagtag ccgttttctt ttgccaaacg ttctgcttct
     7441 ctgattgatc cagaaatacc atccgcacca ggagtcaaaa tcaactctgc gccgtaccct
     7501 ttcatcaata aacgtctttc gatactcatc gtctcaggca tcacgatgat tactttatat
     7561 ccttttgcaa ctcctaccat cgacaatccg attcctgtat ttccagaagt aggttcaatg
     7621 atagtatcac ctggtttcag tagaccatcg tgttctgctt tttctatcat acttaaagca
     7681 atacgatctt ttacacttcc tcctggatta aaaaactcaa gctttacaaa aacatctgct
     7741 gaatcttctg gtacgatttt atttaatttg acgattggcg ttttgccaat caattcggtg
     7801 actgagttat agatagctgt catcttcaat tcctccttgt ctttgctctt cttacttatc
     7861 ataatacaat ctgttcattt atggcgagtg attcgtcttt gactagcaaa aaaaagacaa
     7921 agatatacca tatatggtaa t
//
LOCUS       DACDTO010000111         7697 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDTO010000111 DACDTO010000000
VERSION     DACDTO010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.111"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..179)
                     /locus_tag="IWK70_002572"
     CDS             complement(<1..179)
                     /locus_tag="IWK70_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9615067.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAIN"
     gene            complement(192..1295)
                     /locus_tag="IWK70_002573"
     CDS             complement(192..1295)
                     /locus_tag="IWK70_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9615068.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1385..2320)
                     /locus_tag="IWK70_002574"
     CDS             complement(1385..2320)
                     /locus_tag="IWK70_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9615069.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2622..4556
                     /locus_tag="IWK70_002575"
     CDS             2622..4556
                     /locus_tag="IWK70_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9615070.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5013..5429
                     /locus_tag="IWK70_002576"
     CDS             5013..5429
                     /locus_tag="IWK70_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9615071.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5443..6009
                     /locus_tag="IWK70_002577"
     CDS             5443..6009
                     /locus_tag="IWK70_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9615072.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6032..7018
                     /locus_tag="IWK70_002578"
     CDS             6032..7018
                     /locus_tag="IWK70_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9615073.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="IWK70_002579"
     CDS             complement(7173..>7697)
                     /gene="scm"
                     /locus_tag="IWK70_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9615074.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2203 a         1473 c         1591 g         2430 t
ORIGIN      
        1 ttattgatag cggtagtcgc taaatcaaaa actagcattc caataaaaaa taaaaaagtg
       61 tttagccaat gtatctcatg aaaaaaagcg atagagaata atacccctac cgcaaaagga
      121 aatacactag ccacctttgt tcgtatttca actacttcta aaaagatttt tagtgtcatg
      181 gttcgtactc cttattttag ttcagaacgg tcacccatcg tgtaatggga gtcctccgat
      241 aatttgaagg tgtctttgat tggatcggtc acatatactt tatcgtcttt tgtcacgaag
      301 atcgcttccg tcccgttatt caattcgctt tcgacgtatt ccagtccttt tttcacaccc
      361 attgaaaaga ctgctgtaga caacccgtca ccatcaattg atttatctgt tatgatcgtg
      421 acacttgcga tatcattgtc gaacggataa cccgtttttg gatcaaacaa atgatggtat
      481 tttttgccat ctacttctaa ataacgctcg taaattccag aggtaactaa tgttttgttc
      541 cgttctttaa tagaacctaa aacagatccg cgagccatgt tcggatcttg gatgccgact
      601 gtccagtctt ggttttcgcc tcttgggctg tggcctaaga catagacatt accgcccaag
      661 tcaacgatgg cagtggtcac tccttgtttt ttcaagactt tcactacttc gtccgtaatg
      721 taacctttgg cgattgcacc taaatcgatg atcattcctt tttcttttag ataaacagtt
      781 ttttctttgt catttagttc gatcttatga taatccacta atttcaatgc ctgatcgatt
      841 tcttcttggg aaggttttct ggcgtcatca aaaccgattc gccataattg tgtgattgca
      901 ccaatcgcca tatcgaatcc gccttgagaa tcttggctat attcgtaggc ccgcttgact
      961 aacgtgtaga cgtcatcaga aacttttact ggtttgatcc cagcttgctc attcacttca
     1021 tcgatctcag aacctttttg attgatcgtg attttatcac caagttcttt tactcgatcg
     1081 aaggcaggtt tcaatgctga ttctttgcct tcatcataca cacggatacg aacgtaagtc
     1141 cctaacaaga actgttcatc agaatatgga tctttcaata tcttggtagc tgctgaactt
     1201 tcagtggttg ttttatttgc tgagttgctg cagccagtta gcaccataaa agagatgccc
     1261 agtgtgatta aggaaaatag tagttttttc cgcatctttt tcctctcttc attttgaaag
     1321 gtgagacgac tgcctaatct ggctatcgtc ccaccctcca acttttatta gtatgattat
     1381 gtgattattt ttctgttacg atattatcta cttcgattgt ttgagtgtcg cctttttcag
     1441 ctgcattcac taattgttgt gcgtacatga tgaatgaatg tgagctgtgt gtagcacctg
     1501 taacaacttc tacattagca ggtgagccat cttcttcgcc cattgctttg acgaattcgt
     1561 cgttcagagt agggatgtaa gtttttggtt ctgtaccgct ttttgctttc atattttcat
     1621 tatattctgt atcatcttgt tttgatttac cgtctttgtc gatataatca aagtttgatt
     1681 cagttacttt gccgccagca actgtcattt cgaattgcac acggtagccg ttagagtagt
     1741 tcttttcttc aagcttgtaa gtgccgtctt tcaaatcagc accattgtcg atttcgattg
     1801 tatctgtgtt accagcttga gcagcttgga tcaattgttg tgcgtagttt tggaagcttt
     1861 cagatgagtg agtagctcca gttacgactt ccacaccgct tgcgctttgt gcttttaaga
     1921 attggtcatt caactcagga atgtattttt ctgggtttgt gccagatttt gcttccatgt
     1981 ttttgttgta ttctgcatct tttgtttttg attcgccgtt ttcattgacg ttatcatatt
     2041 ttgattcagt gattttgccg tcttttacaa ccatttcaaa gactgcacgg taaccatttg
     2101 aataattttt ttcttctaat ttatatgtgc cgtcttgcaa atctccgcca gctacaactt
     2161 ttgcagatga ttctgttgtg ctttttgcag tcgaagattg tgctgtagat gattcagaag
     2221 aagagtttga gcttccgttg tcatctgcgc cgcaagcgcc taacactaga gctgaaaatg
     2281 ctaatacggc aaatccagtt acaaaacgtt ttttcttcat tttaataccc caccattcca
     2341 tttctagtcc acaatttttc tttgtgattc attatacatt ctaatctttt tagcctataa
     2401 gtcaactatt tttgataaat aacccatgta atatttcaaa cgttttcttt cagcaaattt
     2461 cgtgaaaaaa cgctttattt taaaatttga aaaaaatcca aaaggagtta gacaaaagaa
     2521 cagaacatct atataatata tggagattgt gtaataagta tcaaactaaa agaattatca
     2581 tatattcatt atatagatag tctataagga gcggatgatc aatggcaaag caaaatattg
     2641 tcgttgtagg agcaggatat gccggtgttt cagctacaaa atacttggca aagaaactga
     2701 aaaaagaaga tgtagcaatt actctgatcg atcgtcattc gtaccatact atgatgaccg
     2761 agttgcatga agtagctggc ggacgtgtag aaccagaagc aatccaatac gatctgcagc
     2821 atttgtttgc tcgtcaaaag aatgtacaac ttgtcacaga cactgtaatc ggtatcgata
     2881 aagaacgaaa aatcgtcaag accaaattag gttcctatcc ttttgatcag ctgatccttg
     2941 gaatgggcgg agagccgaat gactttggta cacctggtgt caaagcacac ggattcaccc
     3001 tttggtcatt tgaagattct gtaaggattc gggaacatat tgagaagaca gtagcaaaag
     3061 ctgcattgga accagatgat gaactgcgta aagccatgct gactttcgtt gtctgtggtt
     3121 ctggatttac cggtattgaa atgattgggg aactgatcga ctggaaaaat cgcctagcca
     3181 aagattataa attagatcca aacgaaatca cactgatggt cgtagaggca atgccgacga
     3241 tcttgaatat gttggatcga aatgatgctg caaaagctga acgctatttg cgtaagcaca
     3301 acgtggaatt attattgaat gcaccaatcg tagaagtggc tgctgatcat atcaaattga
     3361 aagacggacg caacattccg actcacacac tgatttggac agctggtgtc aaaggaactt
     3421 ctgacgctgc tgactttgga ttagaatcag cacgcggaca gcggttgatc gccaatgaat
     3481 atatgcaggc aaaaggctac gaagataaaa acatctatat cattggtgat ttagtctttt
     3541 atgaagaatt cccaggaaca ccgacaccgc agatcgttca agctgctgaa caaacaggac
     3601 acacagccgc agcgaatatc gtggcttcga tcaaaggcgg agaaaagcat aaatttaaag
     3661 gaaactatca aggatttatg gtttctattg gtgccaaatg gggggtagct aacctatttg
     3721 ataagatcca tttaagcgga tttttagcta tcgtaatgaa acatatcgtg aatttgaaat
     3781 acttctttga tattcgttct ggttattata tgttccaata tttaatgcat gagtttttcc
     3841 atatcaaaga tgatcgcaac gtgactcgag gacattcttc tcgttacggc aatgtcttat
     3901 ggagtgtgcc acttcgtatt ttctatggtt tagtttggtt cgttgaagct tccaaaaaaa
     3961 tcattgggaa tggtgaagtg ttcaaaccaa gtacttggtt tggtgaagga tcatggttca
     4021 ctggaaacag tgtgttccct tggacatggg aatacaatgt aacagatgtt gccagtggtg
     4081 cttctgctgc tggtgatgca accacagctg ccagcggagc aggggcaagt gccggagaag
     4141 cagcgactca agctgcacat tttggtttga gttatgcata cggtgaagaa ccaatggctg
     4201 tgattgagaa gtcaccagac tggttcacaa agatcatgca agtgatcatg ccaaacaatg
     4261 atgtagcaat gttcttccaa aaattcatgg tattctttga aattgccttg gcattagcat
     4321 tgattgctgg attattcaca tggctatgca gtgcgacaac gattgtattg gtgatcacgt
     4381 tctgcttatc aggcatgttt tactgggtaa acatttggtt catttttgtt gctttcgcat
     4441 tgatgaacgg ttccggacgt gcgattgggt tggatcgctg ggtaattcca tggctacaac
     4501 gtacactagg taattggtgg tacggtagac caagttccca gtacggcaag aaataaatag
     4561 ataaagaacg ttatttgaat gcaagatttc aatagaaagg ggctgagaca aaccgtcaca
     4621 gcctcctttt tatgtaaaat taaaggaata agatgcccga gataagcctc catgagcaag
     4681 acacaggtgt cacaacctct gttacaacct cttgttataa acggtgttcc agaagtagct
     4741 tcttcgaaaa ataagccgaa atttcacaaa aacttgaaaa gtaattttcg taaattcctt
     4801 cttatttctt gaagttgccc acttctgtca caacctcttg ttataaacgg tgttccacct
     4861 gctgcttctc ggtaataagc ccaaagaatt agaaaacatg gaaagctgtt ttctattctt
     4921 cgttcttatt actcaaagca agacacaggt gtcacaacct cttgttttta tgctaaaatt
     4981 gggtgagtta atctggaaaa aggagaacaa aaatgagcct taaagaattt gctgaaaaaa
     5041 gtctgctcaa accttgggat gtttttatta ttttgctttt gactgttctt tcttttttac
     5101 cagtcatgat ttttggtctt cagcaaaaca ccgcaacagc agaaaaagaa gcggtactgc
     5161 gtgtggatgg cacagaaatc aaaacattcc ctttgatggc tggagaagag agcaacacat
     5221 atacttacaa agatgaacac ggagattata acttgatcga gatagatggc gatcgagtac
     5281 ggataaaaga agcagattgc gatgaccaga tctgcgtacg tcgaggctgg gcaacgaaaa
     5341 atggcgaaac catcgtctgt ttgccacata aattagtgat cgaagtccgt tcaacagatg
     5401 gaggtgacga agacagtctg atttattaga ccttcacaca ccatgagtaa attacaaaaa
     5461 atgatttata tttcgctact tgttgcacaa ggagtgatca ttggtttatt ggaaaatatg
     5521 atcccttatc ctttttcttt tgcgcctggt gcaaaactag gcttagccaa tctaatcacg
     5581 atcgttgcca tttttaccat gaaaaaacaa gacagcttct tactactttg gctacgacta
     5641 ttcctgacta ctttgctagg cggaacgatt tccacattcc tttatagtat gagtggtgct
     5701 ttgcttagtt attttggaat gttattggtc aagcaattag gaccaaaacg tgtcagtatc
     5761 atcggaatta gtgcggccgg cggtttcatg cataatgtcg gacagctttt gatggcttct
     5821 ttcattgcac aatcatggac agtcatgttg tacttgccag tcctttcagt tttagggatt
     5881 ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt tacgacatgt gcagacactt
     5941 caacgattcc aaaaagatta tgagcaggga accaaacaaa aatggagccc aatgtttacc
     6001 aaaaaataat cgtcacacaa ggagtttttt tatgaagcta catccaatgt gggaaaacta
     6061 tcctgcttta agtaaagaat tgacagaaac actaaaacgg atggaagaaa ctgtccgcct
     6121 gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc catgccggcg gaaagctgtt
     6181 gcgtcctgcc tatcagctat tgttctcaca atttggacca gagcaagatc gagacaaagc
     6241 catcgctttg gcagcttcta tcgaaatgct ccatacggcc acactgatcc atgatgatat
     6301 cgttgatgaa gcggatctgc gacgtggtca gcctaatatc cgtagtcagt ttggtaatgt
     6361 cgtttctgtc tatgcaggag actatttatt tgtctgctgt tttaaactat tgtctgatta
     6421 ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt atggaaaaag tattgaatgg
     6481 cgaattaggg cagatggatg atcgctataa ttatgaactt tctgtaaaag aatatttgga
     6541 aaatatttca ggtaagacag ctgaactgtt ccaattaagt tgttctgttg gcgcttttga
     6601 aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat atcggacttt ctatcgggat
     6661 ggcctttcag attatcgatg atattttaga ttatacaaaa gagagccagg aaatcggcaa
     6721 acctgtacta gaagacatgc gtcaaggggt ctattcttta ccgttgatct actctttgca
     6781 aaaaaacaaa ccaaagctac taccttattt agagaagaaa gcggcaatga cagaagaaga
     6841 tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc gtggaagaag ctaggaagtt
     6901 agcggcctct tacacagaaa aggctttgaa ggaaataaag aaattacctg ccacctctct
     6961 tcgaacaaaa gagaatctct tttcccttac ccaactgatt ttggatcgca aagactgaat
     7021 agggaaaggt tcgatacgta aataaatttc tcagaaggaa atctacgtgt tttttgacaa
     7081 attgaattgt agttcttgta tttatgataa aaagcaaaaa taaagagcaa cgtccgttag
     7141 cgacgttgct cttttttatc ttgaatattt ttttaccttt tcgatttctt ataactcata
     7201 aaagtgattc ttaaaccgat tacaagtaat ataacgcctg cgagtgttat ccaattattc
     7261 gattgacttc cagttttagg aagtgctctt tttgatttgt ttccattgtc attgcttgtt
     7321 ttattagtta agttaaagtt cgtcctgtta tccgaacttg tttttgagaa ggagacactt
     7381 gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac tatcgcttga agttgatgaa
     7441 tctgagctat cacttgtggt agaggatgta tctgtactgt cacttgaagc tgacgtgtcc
     7501 gtgctatcac ttgtagtggt agatgaatct gagctatcac ttgtggtaga ggtagtatct
     7561 gtactgtcac ttgaagctga cgtgtccgtg ctatcacttg ttgtggtaga tgtatccgta
     7621 ctgtcgcttg aagctgacgt gtccgtgcta tcacttgtgg tagaggtagt atctgtactg
     7681 tcacttgaag ctgatgt
//
LOCUS       DACDTO010000112         7600 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDTO010000112 DACDTO010000000
VERSION     DACDTO010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.112"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWK70_002580"
     CDS             complement(138..428)
                     /locus_tag="IWK70_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615075.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWK70_002581"
     CDS             complement(546..806)
                     /locus_tag="IWK70_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615076.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWK70_002582"
     CDS             complement(943..1356)
                     /locus_tag="IWK70_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9615077.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWK70_002583"
     CDS             complement(1812..2297)
                     /locus_tag="IWK70_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615078.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWK70_002584"
     CDS             complement(2436..2651)
                     /locus_tag="IWK70_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9615079.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWK70_002585"
     CDS             2875..3675
                     /locus_tag="IWK70_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9615080.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWK70_002586"
     CDS             3694..5562
                     /locus_tag="IWK70_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9615081.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IWK70_002587"
     CDS             5555..6046
                     /locus_tag="IWK70_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP9615082.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IWK70_002588"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IWK70_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9615083.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IWK70_002589"
     CDS             6606..>7600
                     /locus_tag="IWK70_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9615084.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2708 a         1213 c         1442 g         2237 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACDTO010000113         6793 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDTO010000113 DACDTO010000000
VERSION     DACDTO010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.113"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(4..1029)
                     /locus_tag="IWK70_002590"
     CDS             complement(4..1029)
                     /locus_tag="IWK70_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9615085.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            complement(1274..2545)
                     /locus_tag="IWK70_002591"
     CDS             complement(1274..2545)
                     /locus_tag="IWK70_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9615086.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2829..3776)
                     /locus_tag="IWK70_002592"
                     /pseudo
     CDS             complement(2829..3776)
                     /locus_tag="IWK70_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
     gene            3788..4261
                     /locus_tag="IWK70_002593"
     CDS             3788..4261
                     /locus_tag="IWK70_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9615087.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            complement(4434..4988)
                     /locus_tag="IWK70_002594"
     CDS             complement(4434..4988)
                     /locus_tag="IWK70_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9615088.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(5461..6630)
                     /locus_tag="IWK70_002595"
     CDS             complement(5461..6630)
                     /locus_tag="IWK70_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9615089.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT         2186 a         1227 c          992 g         2388 t
ORIGIN      
        1 tttttaatcg atattttttt cactttctct aacaataata ttattagaaa caatcacttt
       61 gatcgaaaca tttctaactt cttttattcg ttcattaacc atgcgaactg ctagttttcc
      121 aatttcttct gtattcacat tcaccgtatt taatgcaggt gttaaaaatt cagcaacttc
      181 aatattatca aaactcacaa tcgaaatatc ttctggaata tgtagaccat ttttttgaag
      241 tgaacgataa acccccacag caataggatc atttcctacc atgatagcag tcggcaactt
      301 atctttagca tgttttttca aaagtaaatc tgctgcctgc attccgtcaa gtgtggtcca
      361 gccttctaaa taaatttgac tatatttttc taatcctttt tgtttcatcc acgtctcata
      421 ggctattttt cgataatcat catctgacac gtgtttattt ccttggctat ctaaacgaac
      481 acgtcctcct ccgataaacg caatgtttcg gtgtcctttt ttatacaatc gatctaaatg
      541 ctgattcgtt gcttttgtta aatcagtata aactgcatct acttcagctt caatactagg
      601 gtcatcaatc actacgacat tcgaattaat tgctgatact tcttcaatca cagaagatag
      661 cacctgccca ataatcaaca caccttgaca ttttagcaaa tcgtcttgct ccaacggtgt
      721 ttctgacaaa cgaataattt tcttcaaatt gactttgctt tgttctgctt cgacttgaat
      781 ccctctgcga atcgttcgaa aatagggatc ttccaactca tctacttcag aaactgtcgt
      841 tattaatgca atatttgtta aattttttgc aacgggactt tttttgacat aatgataaaa
      901 attaaccgat tctgcaatct ttcgtttggt ttcatcggtt actgaaagtg tcggatcctc
      961 atttagcacg cgcgatactg ttgcaatgga cacacccgcc cgttttgcaa catccctaat
     1021 tcctaccata atagctcctc ttttcatatt tgaaagtgtt tactaacata taatcataca
     1081 ttttgaaaat aagcaaattt cgttaaaaat aggcactgta atcataatta aaaacaagaa
     1141 aaaatatccg aacaatttag agtaaataat gctaattcta gtgaatttgc gacgaggaac
     1201 tgctagatca cagttcattc tctctatcgt tcggatattt tattgttaac tatacttatt
     1261 tcacagagct tttttatttg attttgtgaa cagagtctcg agaaatcaat tctccctcta
     1321 atagtagtac ttgatttttt atttcttctt gtttggccag tccaatcagt tggtgaaaag
     1381 caatcgcccc cttgttacct ggatttagtc gtattgttgt caacgcagga tccattaatt
     1441 ctgcaaaagt aatatcatca aatccaataa tcgataattg ttccatcact cgtttattct
     1501 tattttttaa aaattttact aatttagccg ctaaataatc tccagtaaca acaattgcag
     1561 taaattcagc taattcttga taaattcgct caaattcaga gttgttatat atccaatgcg
     1621 atttaaaggt taaatgatat ttctttaatg tatcgatata agcttgatgt cgttcttgaa
     1681 taacacttgg ttcacctaca atgtagtcaa aagaaagaaa cgctattttc tcatgaccat
     1741 aatgaatcaa atgttccact gccaatgtac tagcttggta atcatttgta tttacaaata
     1801 aacgattcat ttgttgttca agttctctta atgtctcttt tgaaagatgc gtatcaatat
     1861 aaacaacagg accatcaaaa acgttattta attccaaaaa ttcttttaca cgtacaccaa
     1921 caacaatgaa cccctcaaaa cgccaatttc tctgaatagt tttaacatct tctgctgaat
     1981 taacattatg caccaataca aaataaccat gattctttgc ctgttcctca attccagaca
     2041 aaacttctgc ggtaaatgga tcagaaaata caaaatcagc attatcaata gccgaaaaat
     2101 aaagcaatcc tattaaaaat gcaatcgtct taccgattcc ctgacagaca gaacacattc
     2161 cactaggatc attaaatgaa taagcatttg catatcctaa actaggtgtt gcaaaacatg
     2221 aaaacaacac acgaaataac gcattgatgt tagtaatcgt ccctaacgtt gagcgcgcac
     2281 tgccaccaat cttcttttga ttgaccacaa tcgccgtaga aagattatca atctcatcaa
     2341 cctctggacg actgtattta ggtaaaaata aacgggtaaa actatcatag gtgtcattca
     2401 gttgacgacc agcttcttgg gtaatagtat caaaaacaat cgatgatttt cctgaaccag
     2461 atacgctggt aaaaatacta attttatttt tgggaatttt gagactgaca tttttaagtt
     2521 gtttctcttg cgtggataat ttcaatatac tcgttcattt ggtactcccc tttattttga
     2581 aatagttgag aatgtttcaa tgagacacgg tatttttggc agatgtttag tttacctaga
     2641 aagaggtata aaaatcaaga aaaaaagaga ggctgactca taagtagttt tattagtcga
     2701 aaatgatagt ttataaaaat aggaaccgca tcaaatcaac attctttaga atggatttga
     2761 tgcgtttttc tttatttttt gacttatgag tcaacctctt actcttcgtc atggatttga
     2821 ttagctaatt attgctccac ttgttcacga atgaaattta agtactgcgg taaaaataaa
     2881 ggaatttctt caaagcgtgg actttgattt tcatacgctt ttgcatctag gtttccttcc
     2941 cctgtagcaa tttcaatcaa taattgattt gggtcacgat aataaagtga tacaaaaaat
     3001 tcacgatctt ttattcctga atttataaaa ttgcgctctt gtaattgttt gtcgatgcgt
     3061 tgtaaatcag atttattttc tacgccaaac gcaacatgat ggacgctacc aatcccttca
     3121 atggcaactg gattctcacg atcttgaata atctgaactt cttgatagaa ctgagtgtct
     3181 tgattttcta agattgtcac tcgttgatga cccgaaaaga aactgacgta ttctttgtgc
     3241 taccagccta ccgacaataa ctctcgttca gtcgcatcgg aatattgtac gcgtagttga
     3301 atggcatcaa ttcctaaaat ggcatgctct gtagggatct ggtctgtgac atacgggaaa
     3361 taatcatcaa tatcttcaaa ttcgcgtaag ggaactaaag ctaatcgtgt tgcatctggc
     3421 ccttcaaaac gtaagatagg tcgtccagaa aatgattcga ttccataatg gcaaacgcct
     3481 tgtgtttcaa aatatatttc ccaaaaatgc aatgatgctt cggaaggaac ttttagtatc
     3541 gttcgttcaa tcgtattggt tccaaaaaac tggttgttcc cttcttgtac ttcaaaaaaa
     3601 gtcagttcag ttcccacacg gtggtgatta tctgaaaaaa acaggtgata catggtatga
     3661 tcgtcttgat taattgtttt cattagtaat tttaaaccga gtatattatg ataaaagtca
     3721 aatgtcggtc gaataaagcg attcacaata gaaatgtgat gaatacgtgc gggcataata
     3781 tctaaaaatg agaatcaaac ctataaaaga tggtgaacaa cttttgtttt catcagaaga
     3841 aaataagccc ctttatttag actatgtaaa tcacaatttg aaaatcatca ttaaagagaa
     3901 taacttaaaa catatcacac ttcatggttt tcgtcatacc cattgtagtc tgctctttga
     3961 atctggtgcg tctgtcaaag aagtgtatgt tcgacttgga catacagata ttaaaaccac
     4021 tatggatgat atatacaccc atatcgcgaa aaagcagaca gaggaaacgg caattcgttt
     4081 tgcagatttc atgagttcta cagaggaaaa gcaaggaagt ttgcagaagg tcaaaagtat
     4141 ggtcaaaaac gaaaaaagca gactatccct attagatagt ctgctaaacg ttgagttatc
     4201 agtatttttc ttattccgca gcgtttgcgt ttttgagacc gtattgattc tcttttttta
     4261 atttcatcct tttccaacct aaaaatgtta tttaattact tagtttatat aacacgaacc
     4321 tataccaagt accagtaacc ccagctttta ttccgcgaac tttatgaaat aaaatcgttc
     4381 tagataaaaa tagcttttaa tctttgtctt tactgtttat aaaaattttt aaattataca
     4441 cctagtttaa atccacgaag tgatgctcct atttcatctt cagttatacc aatgtacctt
     4501 tttgtaattg cttgcgagct atgattaaaa ataaacatta aggtagcaat gtctctagtt
     4561 ttcttgtagt agtgataccc aaaagttttt cttaatgtat gtgttcctat atcatctcgt
     4621 cctaattttt ctgctatatc tgcatatatc tgatagacac gatcccttgt tatatgtcga
     4681 ccttgttgcc gactcggaaa taaccaatca ttttcttctt taccttcagt atataataaa
     4741 atttcttctc tcaaattatc tataaaaatc ggttttcgct tacctgtttt atgttcaaca
     4801 ataataggct tacttttgcc tcgtatatct ccaactttta atcctataat atctgacata
     4861 cgtaacccat tattgattcc aaacaaaaat aaaaaactat ctctttccgg attacttgtt
     4921 cttcttagcc aaaactgaaa atcttgtatc tcttcatttg tccttaacgg ttgtacatta
     4981 tagctcacta ttgtttccct tcttaaaaat attctgagtt aatgattatg tatatatttt
     5041 accataatca ttaacttata tatataaact tttgaaagtc aataaaatca agggatttag
     5101 tttatgattt tatatcattt cagtaactca gaatattccg acttttttgt acaaaaaaat
     5161 taaccttact tatatgaaga ttctaagctc ttatttaaat tttagacggg cagttatccg
     5221 tagaagttct tagaatagct aatttacata attaaattat atgaaattta agtttgaaca
     5281 atatactaag aactcccctt actataaagt gaaataaatt agcatatgaa taaccgtgct
     5341 accaagcttt tcattaataa aatattttta ttaatatttt ataactttac gcaagatcat
     5401 aattttgtcg ggagcgtcaa taattttgtg taaatggaaa agcccattct gttgatcaag
     5461 ttaaaacatt gattctaatg tatcatgtat ttctttaaaa cctttatgaa ctcggcatag
     5521 aaacttttga ttatagttgt tgaattgaga tactagaaat ctctccagag attcttcatt
     5581 aggaaattgc tcttttcttt tggtatattt ctttaattgt ttgttaaaac cttcaatcaa
     5641 attagttgaa taaatagttc ttctgattga cggagggaaa ttataaaagg ttaatatagc
     5701 aggattcatg agcaatttaa ccactcgtgg atactgcttt ttccatttat ctatcataaa
     5761 ccttatttga ttatttgctt cttcttttga acttgcttga taaactaatt tgaaatcgct
     5821 acagatttct tgtcgatcac taacacgaac cttatgagcg atatttctag atatatggac
     5881 acaacattgt tgaaattgag catttggata aacactatga atactatcgt taatgccact
     5941 taaaccatca gttacgacta ataaaacctc ttttaaacca cggtctttta ggtcttgaag
     6001 aatctctttc caaacataag cagattcagt tggagcaaga ctaaacccaa gaacctcttt
     6061 ggttccatct aatcgaatgc caattacaat ataaactgct tcttttgaaa cagtttgtct
     6121 ttttaaagga atgtgagtag catccataaa aatgacagag tatctggatt ctaaggacct
     6181 ttctttaaag gcaataatat cttcagaaac tattttagtc atatttgaaa tggtttgtgg
     6241 cgtatagtga tgcccgtaca ttttttcaat caattcagag atttcagaca tagtaattcc
     6301 tttttgaaat agctgaataa tagtggtttc taaagagtca ttggttcttt tataagcagg
     6361 taaagtttgt tgagaaaact taccatttct atctctagga attaccaaat ttaattctcc
     6421 atactctgtt ctgaatgaac gtgaataatt cccattacgg gaattacctg aattgaaccc
     6481 agcacgatca tatttttcat aatcaagaaa agctgttaat tcagcctgca ataatgaatt
     6541 aacagctaat tctaaatgac atcgaaataa ttcatctaaa tcacctttat tgattagtgt
     6601 ttccataatt tctgtagtaa aattgggcat gagaaagtcc tcctataaaa tttcggttgt
     6661 ggtaacttta attttacaga atggactttc tttttttcta ccctaaatat ctatttacac
     6721 aaaatatttt acgctatcat tttgtctgtg tagatatttc gttatatcac taaagataat
     6781 aagatttaat gac
//
LOCUS       DACDTO010000114         6706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDTO010000114 DACDTO010000000
VERSION     DACDTO010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.115"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(7..1164)
                     /locus_tag="IWK70_002596"
     CDS             complement(7..1164)
                     /locus_tag="IWK70_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615090.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1167..1523)
                     /locus_tag="IWK70_002597"
     CDS             complement(1167..1523)
                     /locus_tag="IWK70_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615091.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="IWK70_002598"
     CDS             complement(1821..2795)
                     /gene="sgrA"
                     /locus_tag="IWK70_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9615092.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(2991..3830)
                     /locus_tag="IWK70_002599"
     CDS             complement(2991..3830)
                     /locus_tag="IWK70_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9615093.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            4015..4902
                     /locus_tag="IWK70_002600"
     CDS             4015..4902
                     /locus_tag="IWK70_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9615094.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(5486..6181)
                     /locus_tag="IWK70_002601"
     CDS             complement(5486..6181)
                     /locus_tag="IWK70_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9615095.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(6165..6563)
                     /locus_tag="IWK70_002602"
     CDS             complement(6165..6563)
                     /locus_tag="IWK70_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9615096.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         2108 a         1319 c          987 g         2292 t
ORIGIN      
        1 cttttcttaa ttaacgaaat ccaatttttc caatatggat ctatttttct cattcctgat
       61 ttttatgatt tcagctttca gacgcgccca atcatttttt gtaggttgtc cattccagta
      121 aaatatctgt gtattcgttt gatcatatgc ctttttgtcg ataggataat cgctaatgac
      181 aatatcaggt aactgatcag cggatgcact atattttatt gggcgttcag tcatagataa
      241 tatccttttc tttgcccata attcctgtgt tttaccatac tttgattgaa tatagaatat
      301 gacaggttgt tcgtgtttta taaaaatatc cctgactaac atacagtatt gaaaaatcaa
      361 ccgtctgttc ttttcataat aagtattaag ctctggaatt ctgcgagcta ccccactaaa
      421 aaactgttgc atgattggat acatcactgg aaatatatgc tgaaaatctc tgtcttctgc
      481 tgattttcca aaagcatcca ccttcttttt tgtcccaaaa acccttgaat acaaatggat
      541 cattgccaaa ttgacatata gataattttt ttctgaagtt gtaaaacaaa tcttgaagtt
      601 tttttcaaaa ttttctacaa tagacgattc aatccagccc caactttctt gtactttatc
      661 tggagtatcc aacaaatctt cttccaaata ggtgttcatt acaccaaaca taaaataaag
      721 gtaagcttgc tctgttggag tgatgtgatt catcgaccag aagtttgctt caaaaaaagg
      781 ctctagtatg taatgcgtaa actcttcttt tgttatcaat ggatgcacaa atttgttcat
      841 ctcttctggg acatacgtaa tcgagtggcc ttgtttcatt cgttgcaacg aaattcttag
      901 taggctgatc catttctttc tatctatact acgcgtatat ggcgcatgtt tcgcaaattt
      961 ttctaatagt tgacgtttat tttttatact tgataatata tcagaatgat tttcgtcgaa
     1021 tgactgccaa tacatcaagt gaaaaaagta tcggatacgg tattcttctc cttctatcgg
     1081 attacctttt ttcaagctgc aagataaatg atagtttttc aaaatagtat taagttctct
     1141 taattttctt tgaatcgtac gcaaacttat ataatttttt gactaagatt ttctaaggat
     1201 tgataagtac ctttgtaaag tgaatccagt aaaatataac caatcgttcc ctctaaaagt
     1261 atagaataaa ctttactcag taaaaactga gaagaaaact cgacttttat catcttgttt
     1321 tctgaaagaa aagataattt atattctgga tacctttcgc aaaacgattc tgcttgttga
     1381 atcaccgaat tcatcttagc cagggtgtag tttagatcta aagcgatatc cttcaccaca
     1441 tataatttct cgtctctttc ataacaatag ttcaaaaatt gatatacttg cctttcattg
     1501 atatcaataa attcttcaaa caaagtacat actccttttt atctattatt tagacttatt
     1561 ccgacagcta catgaataac gtacaaatag attcgttact gatttttcag agatactacc
     1621 atgcgcagtg tgcattgtca attattcttg ctcttatttt ccatttgtgt tcctgttatt
     1681 tcctgtttct caaatatgtt agtaaacaaa aatagatctg gcaaagctac aacttcgcca
     1741 ggcaatacag cactctcgaa tgaaatattc actcaaaaat ctatcgtttt attcaatttt
     1801 agatctgttg cttttattct ttatttcact cgatggaaga gagaacgaaa atagcttgct
     1861 cctacaatca aaatgagtaa cacacccatc catgtaatta gcttgttcaa ggttctacta
     1921 ccagtttctg gtaaatcgct ttttctatta tctgtttctt tcgctttttc ttgatgatca
     1981 attccacgag aaacaaaatc aggctgtttt agcacacttt ttaggttgct ccctgcattt
     2041 ctcttttgtt ccttagttgg tatgataggt accaaatcaa gttcattatt tacttttcct
     2101 gtgctgcttt cgctgctcgt gcttcctgtg ctactctcac tactcatgct tcctgtgcta
     2161 ctttcactac tcgtactctc cgtactgctt tcgctgctcg tgcttcctgt gctactttca
     2221 ctactcgtac tctccgtact gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc
     2281 gtactctctg tactgctttc accgctcgtg ttttctgtac tgctttcact acttgtgcta
     2341 tccgtactgc ttggcggtcc aacaactgtc caactgcttg gtttcaaatt ctccgtgatc
     2401 gtcatctgtc catcataaaa aacaatcaaa tcaactttat ctttactctt gatctcatca
     2461 atcgatagtt ctgcagaaat cattattgta tactcaatac cagcggtcca ttgtacgtct
     2521 tttaaataaa cttccccaat aactttttct gaacttccat taccatccca accatatgtt
     2581 agaacagcat gtacatcact agcattcacg cttttgtttg gtttgactgg aatagtaatg
     2641 acttgttgtg tcatgtcata aacaatccga tcagttttca cagtaaatga aagattctca
     2701 tttgcccgtt catttgcaga agtactgatt ccaatcattg ctgcaattat actcagccca
     2761 agaataacaa ataatacggt cgctgttttc ttcatttcta tcactctcca tataattacc
     2821 ttctatttga taaaggaatt atataaaatg aaaatgatga gaacacgaca gtcagttgtg
     2881 aaaatctaac aaaagatgtc gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa
     2941 tgacagactt aaaaaagcaa gaccgatatc agccttgctt tcttcatctt ttattgataa
     3001 acagcagtgt atttcttcat ccgctttgtt ttatatgcat agagtaacaa accaacaatc
     3061 aagactaaca agaataatgt agttgatata gatgcttcag ccccaaagtt tcccccagta
     3121 attattgtat tttgcgattg gatcagagtg gagaaaaaac cttgttcttg cgttccgctg
     3181 actggtatac ccaaaatagc tccttgtgtc caattccaag taatatgagc accaatagcc
     3241 gtaaataaac ttcctgtact gtagataagt gcgctgaaaa agcaacctgc taaaaagata
     3301 ttgaaaactg ccagaaatgt catgttagga ttcaatccgt gcaaaagagc aaataatgca
     3361 gcagatccaa tcattgctat taacggacca aatctaagca tctgatgctg aaaaaagcca
     3421 cggaataaaa gctcttctga tgcccccact gcaataaata aaataaaact cgttatcagc
     3481 ggcacaatgt ttgcgtttcc tgaatctaac cgatacgccc ccgtcaaata taatggacta
     3541 gtaataagca gaaacactcc aattccaaca acgagacctc ccgctaaata cccccagtct
     3601 tttcctcgaa cagaaacacc ataatcttct attctttttc cttcgacctt actagcaaga
     3661 aataaagcta gtaaaaaaac agctaaatgt ccgaccgtac cggcaattgc tccaatccat
     3721 tcactatttc caataaacat atccgcaagt gcattattga caagaattcc taaaacaaaa
     3781 ccaactaaga ctattaaaac agatccaatc aaccgaataa tcctgatcat ctttcatccc
     3841 tcctttatat taagtttaac aacccttttc tttcttgtct cgtgaaaact atgtaaatgt
     3901 tctccatcat agagagaata gatttttacg aactaagaat atctacagca accttacgaa
     3961 aatgaaagac tgtctcccta attcttctgt tagactgttt ttaataagga aaggatggga
     4021 aattttttga aaaaacaact actaattatg tgcctttcag catgtgcaat tttactattc
     4081 tcaggttgta gcaagtccaa tgaaacgatt gcaacgttta aaggtggaac acttacagtt
     4141 caggattttt atgataaccc aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg
     4201 attgtcttca aagcatttga tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca
     4261 aaagaatata atgaacaaat caaaaaactc ggtccaaact acaaggaaca attaaaagca
     4321 gttggccaaa cagaagagac atataagaaa ctctttaaac agatgctggc tttccaatac
     4381 ggtttgaaag ccaatgtaaa attaacagat aaagatctag acactgcttg gaaagaattc
     4441 tatccagaag ttagtacaca gatcattcta ttttcgacta aagaagaagc agataaagca
     4501 aaaaaagaag caaatgaagg ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat
     4561 aaaacattaa aggaaactga tggaaaaatg aattttgatt caaccaatcc agagattcct
     4621 actgaagtaa aaaaagcagc atttaaacta aaaaacgggg aagtttctga tatcattcct
     4681 gtaacagatc caacgactta tcagcaaagc tattatctag taaaaatggt aaaaaaacag
     4741 gataaaggtt ctaataaaga caaatacaaa tctgagctag aaaaaattgc taccgaagca
     4801 aaaactcttg atacggaatt catggataaa acaatccgta aagtgatgaa aaaagacaat
     4861 gtaactatta aagatcctta tgtaaaaaat atattcaaat aaacaaattt gacccaattg
     4921 gggatgtggc aaaatttgag aagtgatttt tggaaattcc ttcttatttt tgaggcaaac
     4981 cacttttgcc acaacttcat cggggccttt ttttatttgt accaaaacaa atcactggat
     5041 ttgttacaat catgaggata taatttaaaa aagtttttta tttctatcac atgaatcaat
     5101 gcgccattaa gtagattctt tcatgcttcc ctattttttc acttgccgta tcgcttcaaa
     5161 aacaactatc ataaactcaa actcgactga aaagttattt taaaaaagac taaaaatatc
     5221 ccttctggac ttgttcctct tattctgtta tactagagaa acacgaaaga tatttttatg
     5281 cgacactttt catttgtttt tatgaaattt attcaaaact ggcgtaactt cagagttaca
     5341 aggatgtatt agaggttagg gaatacatcg tttaaatgag aaataagtaa tagtcaccca
     5401 aactttaaat taatgaatat tgaatcgtca gatagcctcc tcgagttttc gagaaggcta
     5461 tcgtttattt tatggagaaa ttgattcaga agtaatcagc gacctttttt agttcaccaa
     5521 tcgtttctgc ttggaaagtt tgcacccaat cttcttggaa gcccctcact gctttatcaa
     5581 tcgagggcat ctctaatact tgatctagta gctctttacc taacgttatc gcatgagctc
     5641 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
     5701 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
     5761 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
     5821 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
     5881 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
     5941 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
     6001 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
     6061 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
     6121 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
     6181 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
     6241 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
     6301 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
     6361 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
     6421 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
     6481 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
     6541 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
     6601 tttttattca aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat
     6661 ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DACDTO010000115         6320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDTO010000115 DACDTO010000000
VERSION     DACDTO010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.116"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            59..478
                     /locus_tag="IWK70_002603"
     CDS             59..478
                     /locus_tag="IWK70_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9615097.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            490..960
                     /locus_tag="IWK70_002604"
     CDS             490..960
                     /locus_tag="IWK70_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9615098.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            977..1756
                     /locus_tag="IWK70_002605"
     CDS             977..1756
                     /locus_tag="IWK70_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9615099.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            1743..2564
                     /locus_tag="IWK70_002606"
     CDS             1743..2564
                     /locus_tag="IWK70_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9615100.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            2581..3630
                     /locus_tag="IWK70_002607"
     CDS             2581..3630
                     /locus_tag="IWK70_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9615101.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            3643..4647
                     /locus_tag="IWK70_002608"
     CDS             3643..4647
                     /locus_tag="IWK70_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9615102.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            5280..6299
                     /locus_tag="IWK70_002609"
     CDS             5280..6299
                     /locus_tag="IWK70_002609"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9615103.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         2081 a         1024 c         1382 g         1833 t
ORIGIN      
        1 aattttggca tgcaacttgc ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt
       61 ggatagagaa attatcttag cttcccatgg aaaatttgct tcagggatat tagattcatt
      121 agagctgatc tatgggaaaa atcattctat tactgtattc gattgctata ctgatgagaa
      181 ttttgattta gcggaaacag taaggcagct ttttatgagg tacaaagata aagaaatcat
      241 tgttctgacg gatctttttg gcggaagtgt caacaacgaa ttcttacaat atatcaatcg
      301 ggatcatgtc tatctcgttg ccggattgaa tttgccacta ttgattgagc tagtatctcg
      361 gttggacgct gaagtagaga caactgtttt gatccagcag gtgctaactg attcaaaaaa
      421 aatgatccag ttttgtaacg agcggctcaa tcagaaaata gaagaagacg aattttagga
      481 ggaagaaaaa tgattgtatt gacaagagtc gaccatcggt tattacacgg acaagtagct
      541 ttttcatgga cacaaacaat aggcgcagac tgtattttga ttgctaatga tgatgtacct
      601 acgaatgaga tacgaaagac aacgatcaaa ttagccaagc cacaaggagt caaactagtg
      661 atcaaatcaa ttgatgactc gattgccgcg ttgaaaagcg gggtgacaga taaatataag
      721 ttattcatcg tagtggaatc gatcgaggat gcatataagc tggcagaagc gtatccagaa
      781 atcaaaacca tcaacttagg agggatcaag gcaaaacctg gtactcgcag catcggaaaa
      841 gcggtaaata ttttaccaga agaggaggaa cgcttgaaac gattaattga caaacaaata
      901 gaagtagaaa tccgtcaagt accagcagat aaaaaagtgg atgtaagaga agttttgtaa
      961 gaaaggaaga acctcaatga ttgtacaagc agtcttatta ggaattgttg catttgtcgc
     1021 acaatcagaa tatgcactag ggacatcgtt gctttctcgt ccgattgtga cggggctgtt
     1081 tacaggaatc gtgttaggtg atgtaaaaac tgggatcatt atgggcgcaa ctttagagtt
     1141 agcatttatc ggatcgttct cagtaggtgc ttcgattcca cctgatgttg tgaccggtgg
     1201 gattttaggg acggcttttg ctattacagc tggagccggt acagaaaccg cgcttttact
     1261 aggtttgccg attgctacgt tgacattgat attgaaaaat atttacttag ggcttttgat
     1321 tccaattatg aaccataaag cagacaatta tgcagaagaa ggaaattata aaggagtcga
     1381 gcatatgcac ttgcttgccg gtatcggtct ttccttgatg cttggtgttg tggtaatggt
     1441 ttcttatatg gtgggaagca atacgatcgg aaatctatta aatatgattc ctgacttcgt
     1501 tcaaaagggg ttgtcagtag ctacagggtt gattccagct cttggttttg cgatgcttgc
     1561 tcgtttgctg atcaacaaac aagttgcacc atattttttc ttaggttttg ctatagcttc
     1621 ttacttagag attcctgtga ccggagtagc tatttttggt gcgattttag cagttgtggt
     1681 agtcaatatt atgaatgtcc gtcaactacc acttcaaaca actgcagggg aggtagaaga
     1741 agatgaagaa ttctgagatc aaaaacagca aaataacgaa aaaagagcta aatcacgtat
     1801 tttggcgttc ttttcaaatg gagttttctt ggaattatga acgtcagatg aatatggctt
     1861 atgtttatgc aatgattccg attttgaaaa agttatatca gtcgaaagaa gaaatggcag
     1921 cagctttgaa acggcatcta gagtttttta acacgacccc acatatcgtt acactgattt
     1981 taggaatcac ggctgcaatg gaggaagaaa ataaggatga tcctgaattt gatgtgactg
     2041 cgatcgatag tatcaaaaca tctttgatgg gacctcttgc agggttagga gattctttct
     2101 tttggggaac tttgcgattg attgccacag gtgtgggtac ctctttagcc atgcaaggaa
     2161 atattttagg ccctatcttg ttcattttga ttttcaatat tccgcacatt ctgtttcgct
     2221 atctagcaac aggttgggga tatcgcttag gaacgggttt tttgaaaaag atccaggcaa
     2281 acggtatgat ggaaagtttg acattaggcg cgtctattat tggactgatg gtcgttggag
     2341 caatgactgc cacgatgatc accatcaata ttcctttaaa aatcggttca ggagaaaatg
     2401 ctgtgactgt tcaaagtatt tttgatgata tcgttccaaa tatcttatgt cttggagcat
     2461 ttggcatagt gttttatcta ttgaagaaag aagtgaaacc attaacgatc ttgatcggac
     2521 tagctttctt tggaattttt ggttcgtgga tagggatttt ttaaaaaagg agcagagaaa
     2581 atgtttacga tgcaagatta tatctatgaa gaaaaggaag tactaagtac gatcttgaaa
     2641 aagaatgatt tttcgacaag agaacatatg aaaaaaacag tgaatttact gatcttagca
     2701 acgggttcat cttataacgc ttgtttagca gcgaagccag cattggaatc ttatggtgat
     2761 cttactgtcg atattcaaga acctttttat tttttcaatt atggaaaact ttctccttca
     2821 atcgatactg tgattgctgt gtcacaaagc gggaaaagtg cttctactgt tgaagcagta
     2881 aaaatgatac aaaaacaagg gttacctgtt gttgctatca caaatgacgt gcaaagtcca
     2941 cttgctttag aagctgcaca aattattgat ttaggtgccg gggtagaatc agtaggcttc
     3001 gttacaaaag gctattctgc gactgtttta cagttactac tcttagggat aggaattgga
     3061 ataagtaaaa ataaaatcag caaaaaaata gaacaagact acatgcaaca gttgcgcaaa
     3121 ataatcaacc atttaccagc gattattcaa aaaacggaag aattttttga tggatatcaa
     3181 agtttatttc gtttagcaca gcgtttcgtc accattggat atggtccaaa ctggggaaca
     3241 gctaaagaag cagagacgaa attgactgaa acaatccgcg ttccgtcaca aggatttgag
     3301 ttagaagctt atatgcacgg cccatacctg gaggcagatg cttcgcattt gctattcttt
     3361 attgaagggg aaagtgtaaa taaggaacgt tcgcaaaagt tgcaaagata tatgagtcgg
     3421 tacgttggag agactctcac cattaccaca aaaaaagcaa gaaacgagaa aacacttgga
     3481 ttagcaatag aatgtgatga atatctgtct gtattagcgc ttgtcgttcc gtttcaacta
     3541 ttcgcttata aaaccgcagt agctaaaggt attgacttga acaaaaagat ttttgaagac
     3601 tttgatacag tcttgaaaag taaattataa gaggtggaaa tcatgctgaa attcaatgaa
     3661 gaagaacaaa tcaaagcaat aaaaggcgca ctagctttac gtccgcaagt agaagaaatc
     3721 atcgataaac tttatgcgga aaaatttgac gctgtctatt atctaggaat cggtggaacg
     3781 tatgcttcct cgatgcaagc tgtcacttat atgaatggga aaagcaactt gcctgtcttt
     3841 gtacagcatg ctgcagaata ttacactact ggaaacaaaa gattaacaaa agattctatc
     3901 gttattttgt cctctgttac cggtacaact caagaagtag tgaaagcagt agaacaaatc
     3961 aagaaagtag gagcgacact gattggattt atcgataaag cgaacagtaa gttgtctcag
     4021 ctatgcgatt ttgtggtcac ttatccagct cctggaacag aacaaatcaa attttttatg
     4081 gcagcagatc gattgatgta tttgaatgga gaattcgaag cttattcaga atactatgaa
     4141 cagctcgaac gttatttacc aacagggtta gtggaagcag agaaaaaagc agatgcattt
     4201 ggcttatcat ttgcagaaaa acatcgacgt gatagtatgc attattttat tggagcaggg
     4261 aatcaatggg gagctgttta ttcatatgcc atgtgttact gggaagaaca aagctggctt
     4321 ccatcgaaat ctatccatgc tgccgagttt cttcacggga cgttagagat cgtagaagaa
     4381 acaaccccag tcacattatt tttaggcgaa gatgaacaga gagtgttatc tgaacgtgtg
     4441 gctaaacttt tgccaaaaat ttgcagcaat tatacattga tagacacaaa agactatcca
     4501 gtagaaggta tcagtgaaaa atatcgcgga cgtgttttgt ctttcttatt gatgcatgta
     4561 gtgacacaac gcatcgatgc tcatgtggag cagttgaact gccatccatt agagatacgc
     4621 cgctactatc gtcagtttga ttattaggat agactggata aaaagaatat aaaaaataaa
     4681 gagagcagcg ataactgctc cctttatttt tttaaaatat tggcatcaat tacgttataa
     4741 aacacatttt ttgcattatc tacagttcaa cttataatct gtttaatggg acatgtgggt
     4801 gccaaccatt ttttgttatg caatagtact atttagttgt tgcatggatc cgtagtaaaa
     4861 gtccaagtac aacgagttgc gccagtttac atagcgtttt gtatccagaa tgttgactaa
     4921 aacaaaagtt cctatgtcaa ccgtcaacag agacaatctg tccgttgcag gacctgacag
     4981 atttagaaat gttcaatctg tctcttatgt attttaaatc aatactcact aggagaagtt
     5041 tcctaacctc ctaatagtaa tgctggtagt aacattgtca aaactgcttc ctcttaggaa
     5101 caagcaaagc ataacgagtg taaaaataaa atgtagacta ttgtaacata tcattgatta
     5161 tttcaagtaa aaacgataaa agaagtaaac aaacacttaa aaacaatgga aattagtccc
     5221 aactttaaga aatgtatact ataatggaaa ttatcaatta tttatttgga ggaatatcca
     5281 tgaaacaaaa aatcgcagtt cttggtcctg gatcttgggg cactgcttta gcacaaacat
     5341 tggcagaaaa cgggcatgat gtccgtattt ggggaaatgt acctgaacag attgatgaaa
     5401 tcaataagta tcatacaaat cagcattttt tacctgattt gacgatccca gaatcgatta
     5461 tcggatataa agaattggca gaagcagtgg ataacgcaga tgctatttta tttgttgttc
     5521 caacaaaagc gattcgttca gtcgcacagg aacttattgg taaaatgaat actaagccaa
     5581 tcattataca tgcaagtaaa ggactagaac aagatacgca taaacgaatc tcagaagttt
     5641 tggctgaaga aattccagaa gaaaaaagac aggcaattgt tgtattatct ggtcctagcc
     5701 atgctgagga agtggcagta catgatatca caacaattac agctgcaagt attgatcaaa
     5761 aagcagcagc ctatgtacaa aaactgttca tgaatgatta tttcaggatt tatacaaata
     5821 atgatgtgat tggcgtagaa actggtgcag cactgaaaaa tatcattgca attggtgccg
     5881 gcgctatcca tggattagga ttcggagatg atgcaaaagc agcaatcatg actcgaggat
     5941 tggcagaaat cagccgtctt ggtgtcgcga tgggtgccaa tcctctgaca tttatcggtt
     6001 tgagtggtgt gggggacttg atcgttactt gtacaagtgt tcattctcgc aattggcgtg
     6061 ccggtaactt gttaggacag ggacataagc tggaagaagt tctagaaaac atgggaatgg
     6121 tcgttgaggg tgtggcaacg acaaaagcag cagtagagct agctcaacag ctaggtgttg
     6181 agatgccaat tactcaaaca atctataatg ttttgtataa tggagaagat atcaaaaaag
     6241 cagcaaaaga aatcatgtta cgtgacggaa aaatggagaa tgaattttct cttcgttaaa
     6301 tagatgatca atagttaatc
//
LOCUS       DACDTO010000116         6304 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDTO010000116 DACDTO010000000
VERSION     DACDTO010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.117"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..595
                     /locus_tag="IWK70_002610"
     CDS             <1..595
                     /locus_tag="IWK70_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9615104.1"
                     /translation="PLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVVGYQ
                     FTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELELLQ
                     QIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            616..1149
                     /locus_tag="IWK70_002611"
     CDS             616..1149
                     /locus_tag="IWK70_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9615105.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            1785..2318
                     /locus_tag="IWK70_002612"
     CDS             1785..2318
                     /locus_tag="IWK70_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615106.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            2330..2533
                     /locus_tag="IWK70_002613"
     CDS             2330..2533
                     /locus_tag="IWK70_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615107.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
     gene            2855..3235
                     /locus_tag="IWK70_002614"
     CDS             2855..3235
                     /locus_tag="IWK70_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9615108.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3217..4131
                     /locus_tag="IWK70_002615"
     CDS             3217..4131
                     /locus_tag="IWK70_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9615109.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            4376..5065
                     /locus_tag="IWK70_002616"
     CDS             4376..5065
                     /locus_tag="IWK70_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615110.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(5186..5272)
                     /locus_tag="IWK70_002617"
     CDS             complement(5186..5272)
                     /locus_tag="IWK70_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9615111.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            5948..>6304
                     /locus_tag="IWK70_002618"
     CDS             5948..>6304
                     /locus_tag="IWK70_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9615112.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKAL"
BASE COUNT         2325 a         1103 c         1173 g         1703 t
ORIGIN      
        1 acctttacta aacaatttag atgagtgggt tcgctattca ctccaacaat ttaacgaatt
       61 acaaagtagc tatgcaaaga ctatgtttcg attactaaaa caatatcgaa ctaaaggttt
      121 tgcttatttt tctaaagagg attttcatga actattagat attccaaaaa gttactggaa
      181 caaccaagca aacgttgata atcgtgttat aaagcctata cgacaggaat taaccgcaat
      241 cttcaaagga ttatcaatca aaaagaaata cggaaaaggg cgaggaaaac ctgtcgttgg
      301 ctatcaattt acgtttaaac cagaaatcaa aaaagcagat gattttaata aacatgcacc
      361 aaccaaagaa gaacaaaata aagcattggc tttagctgat aaacaaaaag aatacgaaga
      421 acctgctaca acaattaacg aaaaaatcaa acagaagaaa cgagaacttg aactcctcca
      481 acaaatcaaa caactagaaa aagagaaaga agctcttgaa aaacaaacag agtccgcact
      541 ttcgaaaaaa gagaaaaaaa gtttattatc tgatttagat aatttattcc agtagcaacc
      601 taggaggcaa ccagtatgca accacaagaa acctttacaa taaaggaatt gtcagaactt
      661 tttaagatga gtaggcaagc aatatcgaaa catatccaaa aattagattc ttccatgata
      721 gctaaaaatg agcgtggtta taaggttgta ttgcgttctg gagtattgca acttgctcgc
      781 aacttaggca accaaatgtt gctagatagg ttgcacgagt acgaacaaga caacgataaa
      841 aatgttaata taccaagcaa aaacgtggtt acaaacaagt tgctagatca gttgcgtgaa
      901 gaaaatgaat ttttacgaga acaacttgcc ctcagagagc aaaataattc tcatatacaa
      961 aaactactcg accagcaaca acaactcacc ctacaagcca acaaacaaat cgaaaaactg
     1021 caagaaaaac tacagttgac ttatacagaa gaagctccag agaatgactc aacgactctt
     1081 tctgaagcaa cagaaacaat agacaaacaa attgatactg aaaaaaaatg gtggcaactt
     1141 tggaaataac tggagggaac attcatgaca agaaaaaatc ttctattcgg ttctatttta
     1201 ctaggaattc taggattcag tccgatgtta ttgtcagtga tcaataaagg gaataaacat
     1261 ggtgaattga aattgaatcc aaattattac aacagtccta acgaagaatt gtttgtaaaa
     1321 gaaacagata aataataaaa aagactgctc tttcgagcag ccagttaaaa aataatgagt
     1381 gtttattctt gaaaaagcaa cgcgcttatg aagcgctacc aaaaatttat catattttat
     1441 taaaaataac aacccagtaa aaaatgactg gtaaaaattc acaaagaaac ggaaagaagc
     1501 aaccgcagaa aatcaagctt aaaaagagct tttgacctag accaatcgaa aatcacaaag
     1561 tgagcgcctg tatctattgc tacaggcgct acttggcata gtacacatta tgtcaacaag
     1621 aatttaatat aacatatttt cctaatgcat tctaactata ccacattatc aaaaatataa
     1681 attatcttta ctaaataaaa tattgacaag cctatacctt ttgttttatg attttcacaa
     1741 agaaaacgtt ttctatatat ttttctgtat caggaggaaa ttctatgaaa aaatttactt
     1801 taggggtagc tttgttaact attggggcag ttgttttacc gtcaactact atagtttatg
     1861 cttcagaaaa ttcccaagtg gtatcattaa acgaaaacaa tgctactgaa aatgtagtta
     1921 ttgagtacac tgaagatggt atggtcgtaa ctaaatatga ggatggttat agtgcggaag
     1981 attttgcaaa ttcttcagat atcatgacaa gagctgcagt tgcaagatgg ggcaaaaagg
     2041 aatatacaaa tattgctgta accactggag tagctgctaa tgctattaat gctgcctttt
     2101 gggcaggagg gaaagctagc acttctatgt ttggtatccc aggttgggca attggtgctt
     2161 tgttaacagg tgcaagttgg actaaaaaag gtgcttctcc agggaatgca gttgcaaaag
     2221 aatgggacaa aaatggtaat ggctggattg gattttatta ccaaagggga tatgatgcag
     2281 ctggaagagt tgtagctact agatattcaa cgttataagt taaggagtga tgtttttgat
     2341 atctaaatca ttaaaatcat taaaaacatt tactaaaaag aatccgcaaa tagctactat
     2401 tcgtattttt atatttgcta tagctgttgc tataattgtt aatatgctaa gcggagggca
     2461 attatacaaa tctggtttat ttttagtgtt aatcttagtt gtcttagaca taataaagtc
     2521 taaaaaaaaa taaatcgata cattttagaa gaatagggtt gtttgaatat tacaaagcaa
     2581 caatttcacc ttcggtgaaa taagcgaaac tttacaaaaa gtggctagca aatgctagcc
     2641 tttttttgta ttgtggagcg tggattttga gggggattca agggggagct tgcgctcctt
     2701 tgacaagcgg ttatttgcac caaatacgga ggtatttggc tagcaaatat agagcttgcc
     2761 aaaccctttt agtgagcttt actcattgta cctttttagg tacaatggta ttgcgaacga
     2821 tacgtccgta ggacgtactg aaagggtggg acttatgagc gaacaacatc aaaatttggc
     2881 tagcgatcat tctaaaaaat atacctatcg atccgagcca aaacaaatca gttttcgagt
     2941 gagcgaatcc gaatttgcaa agctgaagca gtcagctgaa gctttgcaaa tgagtgtgcc
     3001 ggctttcgtg aaagccaagg cacaaaacgc gcgtctggtg acaccgaaag tggcgccaga
     3061 cattgcccag gcaatcgcgc gcgatttagc caaagctgga gggaatatca atcaaattgc
     3121 caagtggtgc aatacacacc aacacgacgt tgctcctgat gatgctcaac gtttatccga
     3181 aaacctaaaa atcatgcaaa aggagctaca aaagatatgg caacagttaa agtaagtcga
     3241 accacttcat gtagccgagc aatcaattac gcagaaccac gagcaaccgt caaaacagga
     3301 ataaactgtg acattgatta tgccaaaagt gaaatgaaac aaatacgcac gctttatgga
     3361 aaagacgatc gtgtacaagc acacttattg attcagtcct ttcgaccagg agaaattacc
     3421 gctgaaaaag ccaatcaact aggggcagag tatgcgaaaa aaatagcccc taaccaccaa
     3481 atcgcgattt acacacatac agacaaagac catattcata atcacattgt cattaactcc
     3541 gttaatcttg aaactgggaa aaaatttcaa gcccatgggc aagcattttt aaacaaatgt
     3601 tatgacctaa atgacgagat ttgtcttgaa catgacttaa caatcacgga acgaggaaag
     3661 aaaaccgaaa aacgaaccat gtctgaaatt aaattaaaag aaaaaaatga accggtttgg
     3721 aaagacgaaa ttcggtttgc gattgatcaa acaatgaaaa acaaaaaaac tcgaacgtat
     3781 acccaatttt gtgattcact taaaacattc ggcattcatt gcttgaatcg cggaaaaaat
     3841 tttacttacg aattggtcca caaaaagaaa aaagtacgat ccaacaaact aggaaaggat
     3901 tacgaaaagg agacgatttt acatgagctg gacagacgag aacgaaccta caatcaacaa
     3961 cgaaccaacg agctacgaag agctcgagct agcattcgcg aaactgcaga agaagtacga
     4021 agagaacaac gaacttttac gcctgaacaa tcggaaatta gaccgactcg aagaaaccat
     4081 gaacaacacg ttgaacgagg aacagaccac caacaagaat tatcgctcta aaacgttaaa
     4141 agacatcgaa aacgaaataa agagacaacg tgcctttctt gcgccttata cccaagaaag
     4201 gatacaaact acgatggatt cacaagtcaa aaaactccac caagcgctat tagaccagcg
     4261 aaaccaagaa caaaaaaagg ctttaattca acgatcgatg gatattcttg cccccttatg
     4321 ttgcttagta ctaacattat tagccttttg gttggtatgg ggagaataaa cagacatggc
     4381 attctattta ccctatctac tcatttctgc cagtatttta ggaattattt ggcttagcta
     4441 taaggcttat caaacaagat attcattaaa aggctctaaa attcgattta agcgattttt
     4501 attggtcggg gttatatttg ccttatcaat cgttttaagt ggtttattcg gcgttttaga
     4561 gggtaataaa cgtgtttcaa gaagcatttt attaggggat atcactcaaa agtatgaagc
     4621 agcatgtgat aaaaagaaaa aagagcaagc attagcacaa aaaatggagg aatttaccgc
     4681 ttgttatgag gacatgaaca atatttttgt ggatcaagag aaacgtttaa cagataaaaa
     4741 catgaaaaaa ctcactcgtt tttatcaaaa cttaccagaa aaacagcaaa aagaggtaca
     4801 agaaaaatat gagcaagtaa aaaaagacgt gcaatatttt aaagatacgc agacagaaga
     4861 agcttgttgt aacttattta gcgatacaat ccctttttca accagtgaac aagaacgaaa
     4921 agaacggcaa caatcggtca catacgaacg ttatgaaaca ctgcttcagc aagcaacaaa
     4981 catacaaaat ccaacgaaaa aagaaacggc attaaattat ctagaaagtg tcaaggaatg
     5041 gttggatcag caacaacaaa attgaatttg attagcaatc atgttaaact gaacctgcaa
     5101 atgatcaaac attagataca cgaaagcccc agagaggtcg gattctctgg ggcttttttt
     5161 caggttgatg tcgcctttat tgattttact tcttgtttcg gttattcaac caataactga
     5221 ataaagagac acaaacttct acaagaaggg ggcaaatgaa ccgttcaaac attagataca
     5281 cctccttcca gtcgcaagac tgtcggatag gcgacaagtt attatacctt acaaaataaa
     5341 atctgtgtgg tgtttttaag tatcccttat tttcgggttg ttagggcttg ccctgaccgc
     5401 ctgtaaggcg ctttaagaac aagataaggt tttttaccca agactgtctt aaaacgactt
     5461 agacgagcta tcagaggctt cccccacctc cgttactgac ttgtttttag caaaggagcc
     5521 tttaggcgac tgcgcagtac aatgtgagag catagcgatc acactttttt agcgaccgta
     5581 gggagcaagg ttttgaattg gcagttttga aaaatgcgcg aacctgttgg tagaaaaaag
     5641 aagtggtttt taggcgggaa attttgtcac cctcttttat aatctctttt taaacctttt
     5701 tttaaacctc tttaccgtcg gctgtgtcct tactccttca atggatacag cgttttataa
     5761 actacatttt gtctgctttt aaactacatt ttgtctgctt ttaaactaca ttttgtctgc
     5821 ttttaaacta cattttgtct gcttttaaac tctttttttg cactcacgta tgttataata
     5881 tgcattatca cagacaaaac gttgtttata acgtctgtat ccattgaagg agtaaggagg
     5941 aatatctttg gcaaatgaaa tcgtgaaata ccatcatgaa ttaaatacca ttccgttgcg
     6001 gaaatttaca ccagttgaaa tgaatctatt tttctctatt gtgtctagaa tgcgcgaaaa
     6061 aggcgataaa acagtccgtc ttacttttga ccaactaaaa gatttaagta attataaagc
     6121 aactgctaat aatcgtttca ttaatgattt agaaatgact tatgataaat taatggattt
     6181 acgttttggt aaaagaagtg ctaatgggct cgatatagat agattcgtta tgtttagtga
     6241 gtttaaaata cgtggaactg ctaaagatcc atatgtagac atacaagttt acgaaaaagc
     6301 ttta
//
LOCUS       DACDTO010000117         6173 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDTO010000117 DACDTO010000000
VERSION     DACDTO010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.118"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            725..811
                     /locus_tag="IWK70_002619"
     CDS             725..811
                     /locus_tag="IWK70_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9615113.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IWK70_002620"
     CDS             complement(932..1621)
                     /locus_tag="IWK70_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615114.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IWK70_002621"
     CDS             complement(1628..1906)
                     /locus_tag="IWK70_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615115.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IWK70_002622"
     CDS             complement(1866..2780)
                     /locus_tag="IWK70_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9615116.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IWK70_002623"
     CDS             complement(2762..3142)
                     /locus_tag="IWK70_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9615117.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IWK70_002624"
     CDS             complement(3592..3879)
                     /locus_tag="IWK70_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9615118.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IWK70_002625"
     CDS             complement(3899..4123)
                     /locus_tag="IWK70_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9615119.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IWK70_002626"
     CDS             complement(4763..5296)
                     /locus_tag="IWK70_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9615120.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IWK70_002627"
     CDS             complement(5317..6159)
                     /locus_tag="IWK70_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9615121.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACDTO010000118         6042 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDTO010000118 DACDTO010000000
VERSION     DACDTO010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.119"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(441..1223)
                     /locus_tag="IWK70_002628"
     CDS             complement(441..1223)
                     /locus_tag="IWK70_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615122.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1288..2124)
                     /locus_tag="IWK70_002629"
     CDS             complement(1288..2124)
                     /locus_tag="IWK70_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9615123.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2142..3551)
                     /locus_tag="IWK70_002630"
     CDS             complement(2142..3551)
                     /locus_tag="IWK70_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9615124.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3565..5388)
                     /locus_tag="IWK70_002631"
     CDS             complement(3565..5388)
                     /locus_tag="IWK70_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9615125.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
BASE COUNT         1880 a         1296 c          972 g         1894 t
ORIGIN      
        1 aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg aaaggagtct
       61 aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt ctacatagtt
      121 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
      181 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
      241 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
      301 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
      361 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
      421 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
      481 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
      541 ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag attagctaat
      601 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
      661 ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc gatgaatgaa
      721 tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact cttagatgaa
      781 tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt ctcaccggaa
      841 actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt taaaattccc
      901 acgcttttac taataaattc actataacca gttctttcaa tagagacttc aatgtatttc
      961 acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt tacatctaca
     1021 taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt actgtctgat
     1081 atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc ttctggcaag
     1141 ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac attcacctcc
     1201 gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac aagtctttat
     1261 atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca gtgttagata
     1321 aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact ccttgatttt
     1381 caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca aaaaatcatc
     1441 cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta aatgcgtcac
     1501 aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca gatatcctag
     1561 catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca tatttgcttg
     1621 ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt ctggcaactg
     1681 aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa attcttttgg
     1741 gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt tttgactgcg
     1801 ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat tcagcttaaa
     1861 gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta gatcatccgg
     1921 agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg aatctaattt
     1981 aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt gaaatccaat
     2041 ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa ttaaactgtt
     2101 gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa gccatttctt
     2161 gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa ttctttaaga
     2221 ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc ttgatgggta
     2281 ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc atctgttaaa
     2341 gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg gtcattaatt
     2401 cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat taaaataggt
     2461 aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg gtcaactacc
     2521 cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac accttcttct
     2581 cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc atttttgctg
     2641 gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt accggaggat
     2701 aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt caaatagttt
     2761 tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca gcctctcaat
     2821 gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg atacatagcc
     2881 gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata caagtgaatt
     2941 actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt ttttgaaggc
     3001 agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt gttttgctca
     3061 ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac ctgtacatag
     3121 tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc tagaaaataa
     3181 ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag gcattcatca
     3241 attacttgat gataaaaaga aattcccttt tgattggttt tattattttc aatatccata
     3301 attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt aaacagctgt
     3361 atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc taccccttct
     3421 gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata ggcaccctct
     3481 gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc tgaaagctgt
     3541 ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt gttcctttaa
     3601 gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta ccacaatcac
     3661 tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta ataattgtcc
     3721 tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt ttaaattcac
     3781 ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta gaccaatcgc
     3841 atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa tgctgttttc
     3901 ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag catcgttcac
     3961 ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg cttcggctat
     4021 tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag caatgataaa
     4081 ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca ttggattttc
     4141 tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc tgactaaacc
     4201 gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa ctaatggttt
     4261 tttcagcttc aaggttactc catagagtga aggctcagta attcccatta aagcagtgat
     4321 cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg ctacagcaaa
     4381 ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga tcatttcaga
     4441 acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg tacccgtaac
     4501 caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg ctttgccgtt
     4561 aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg gtccaatgac
     4621 aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa agaatttaat
     4681 aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc aaacagtaaa
     4741 gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc ctagaaaaga
     4801 aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa tgccagcaac
     4861 agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg ctaatagtac
     4921 tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag tttgactact
     4981 gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca tcccggcacc
     5041 tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca atcttgaaat
     5101 aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca cttccttttg
     5161 aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt gtccaccgct
     5221 atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg ctttcctttt
     5281 atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca cattttcttt
     5341 tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta ttctctctcc
     5401 ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa gtgttagttt
     5461 gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc tgtaatccaa
     5521 taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct tttcgtttga
     5581 ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc gtatacttac
     5641 tgctaaaata agtatatcta atattaacta taccagtaaa accattccta cataaccagc
     5701 gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac acaacttcaa
     5761 caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa taaacagcat
     5821 tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca gtaagcaata
     5881 gccaatttca atcattccga gatataaagg tatcgataga atgatttggg ctagtattct
     5941 taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt agtaagtatt
     6001 tcaattccac caattatcaa taaaaatacc aaacatatcc at
//
LOCUS       DACDTO010000119         6002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDTO010000119 DACDTO010000000
VERSION     DACDTO010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.120"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWK70_002632"
     CDS             <1..395
                     /locus_tag="IWK70_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615126.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IWK70_002633"
     CDS             398..1228
                     /locus_tag="IWK70_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9615127.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IWK70_002634"
     CDS             1244..2095
                     /locus_tag="IWK70_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9615128.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IWK70_002635"
     CDS             2092..2451
                     /locus_tag="IWK70_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP9615129.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IWK70_002636"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IWK70_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IWK70_002637"
     CDS             2624..2929
                     /locus_tag="IWK70_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615130.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IWK70_002638"
     CDS             2929..3240
                     /locus_tag="IWK70_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615131.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IWK70_002639"
     CDS             3407..3823
                     /locus_tag="IWK70_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9615132.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IWK70_002640"
     CDS             3823..4491
                     /locus_tag="IWK70_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9615133.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IWK70_002641"
     CDS             4488..4865
                     /locus_tag="IWK70_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615134.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IWK70_002642"
     CDS             4862..5161
                     /locus_tag="IWK70_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615135.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IWK70_002643"
     CDS             5403..5819
                     /locus_tag="IWK70_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9615136.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DACDTO010000120         5902 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDTO010000120 DACDTO010000000
VERSION     DACDTO010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.122"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..986)
                     /locus_tag="IWK70_002644"
     CDS             complement(<1..986)
                     /locus_tag="IWK70_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HAP9615137.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1138..1833
                     /gene="vanR"
                     /locus_tag="IWK70_002645"
     CDS             1138..1833
                     /gene="vanR"
                     /locus_tag="IWK70_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9615138.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1811..2965
                     /locus_tag="IWK70_002646"
     CDS             1811..2965
                     /locus_tag="IWK70_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9615139.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3180..4148
                     /locus_tag="IWK70_002647"
     CDS             3180..4148
                     /locus_tag="IWK70_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9615140.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4141..5172
                     /gene="vanA"
                     /locus_tag="IWK70_002648"
     CDS             4141..5172
                     /gene="vanA"
                     /locus_tag="IWK70_002648"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9615141.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5178..5786
                     /locus_tag="IWK70_002649"
     CDS             5178..5786
                     /locus_tag="IWK70_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9615142.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1781 a         1148 c         1335 g         1638 t
ORIGIN      
        1 tttccgataa ccgtgtattg aaaggcatct tcgaagccat tatccaatat ttcgcaggct
       61 tttgtatatt taggctggtc gatatattca ccgactaagg cattcttagc tgttcgagcg
      121 agttcaatat ccgtaaactt aaagatcgct tttactgctt ctctaaacgg ttttgaattc
      181 ttttttggaa tggaactgaa gatgtttctt aaaaaatgga cctggcatct ctgccaactt
      241 gcgttggtaa atgacttacg aatcgcagac actaggcctt tatgggcatc agaaatgatg
      301 agttctgtcc cctttaggcc gcgttctttt aagtattcaa agaagatgga ccatgtgtca
      361 tcactttctt cattttgaat catgaagcca atgatttcac ggtcgccacc ttctgttatc
      421 ccgatcgcaa tatggcagct tttagaaagc actcgatggt cctcacggac ttttatgtag
      481 agaacatcag tcatcagata aggataattc gtacctgaga gtgaacggtt ctgccattcg
      541 ttgaccatcg ggtctaactg ctcagtcagg ctagaaacaa aagatttcga tacagatttt
      601 ccacatagct cttcaacaat ctttgaaact ttacgtgtcg aaacgcccga aacatacatc
      661 tcaagcattg aagcgagcag tgccttttca tttcgctgat aacgctcaaa caccgtcggt
      721 gaaaattcac catcacgtgt tctaggcact tttaattcga gtgtacccac acgagtcgta
      781 aagtctcgct cataatagcc atttctttga ctctgacgac tttctgaacg ttcatagtca
      841 tcggcttgaa tatattctgt tcgttgattt tccatcaatt ggttgaaaac agtggttaaa
      901 atatttttag aaacatcatc ttttaccgaa tgttcaataa tactttgaac ctcttcgttg
      961 ttcagtgtaa aatgtacttg ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa
     1021 aacattgtac cgtaaaaggg ctgttacatg gccttttatc ttttacacaa ttatatggac
     1081 tttatcctat aatctgataa attattaagg gcaaacttat gtgaaagggt gataactatg
     1141 agcgataaaa tacttattgt ggatgatgaa catgaaattg ccgatttggt tgaattatac
     1201 ttaaaaaacg agaattatac ggttttcaaa tactataccg ccaaagaagc attggaatgt
     1261 atagacaagt ctgagattga ccttgccata ttggacatca tgcttcccgg cacaagcggc
     1321 cttactatct gtcaaaaaat aagggacaag cacacctatc cgattatcat gctgaccggg
     1381 aaagatacag aggtagataa aattacaggg ttaacaatcg gcgcggatga ttatataacg
     1441 aagccctttc gcccactgga gttaattgct cgggtaaagg cccagttgcg ccgatacaaa
     1501 aaattcagtg gagtaaagga gcagaacgaa aatgttatcg tccactccgg ccttgtcatt
     1561 aatgttaaca cccatgagtg ttatctgaac gagaagcagt tatcccttac tcccaccgag
     1621 ttttcaatac tgcgaatcct ctgtgaaaac aaggggaatg tggttagctc cgagctgcta
     1681 tttcatgaga tatggggcga cgaatatttc agcaagagca acaacaccat caccgtgcat
     1741 atccggcatt tgcgcgaaaa aatgaacgac accattgata atccgaaata tataaaaacg
     1801 gtatgggggg ttggttataa aattgaaaaa taaaaaaaac gactattcca aactagaacg
     1861 aaaactttac atgtatatcg ttgcaattgt tgtggtagca attgtattcg tgttgtatat
     1921 tcgttcaatg atccgaggga aacttgggga ttggatctta agtattttgg aaaacaaata
     1981 tgacttaaat cacctggacg cgatgaaatt atatcaatat tccatacgga acaatataga
     2041 tatctttatt tatgtggcga ttgtcattag tattcttatt ctatgtcgcg tcatgctttc
     2101 aaaattcgca aaatactttg acgagataaa taccggcatt gatgtactta ttcagaacga
     2161 agataaacaa attgagcttt ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt
     2221 aaaacggact ctggaaaagc gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga
     2281 cgttgttatg tacttggcgc acgatattaa aacgcccctt acatccatta tcggttattt
     2341 gagcctgctt gacgaggctc cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat
     2401 cacgttggac aaagcgtatc gactcgaaca gctaatcgac gagttttttg agattacacg
     2461 gtataaccta caaacgataa cgctaacaaa aacgcacata gacctatact atatgctggt
     2521 gcagatgacc gatgaatttt atcctcagct ttccgcacat ggaaaacagg cggttattca
     2581 cgcccccgag gatctgaccg tgtccggcga ccctgataaa ctcgcgagag tctttaacaa
     2641 cattttgaaa aacgccgctg catacagtga ggataacagc atcattgaca ttaccgcggg
     2701 cctctccggg gatgtggtgt caatcgaatt caagaacact ggaagcatcc caaaagataa
     2761 gctagctgcc atatttgaaa agttctatag gctggacaat gctcgttctt ccgatacggg
     2821 tggcgcggga cttggattgg cgattgcaaa agaaattatt gttcagcatg gagggcagat
     2881 ttacgcggaa agcaatgata actatacgac gtttagggta gagcttccag cgatgccaga
     2941 cttggttgat aaaaggaggt cctaagagat gtatataatt ttttaggaaa atctcaaggt
     3001 tatctttact ttttcttagg aaattaacaa tttaatatta agaaacggct cgttcttaca
     3061 cggtagactt aataccgtaa gaacgagccg ttttcgttct tcagagaaag atttgacaag
     3121 attaccattg gcatccccgt tttatttggt gcctttcaca gaaagggttg gtcttaatta
     3181 tgaataacat cggcattact gtttatggat gtgagcagga tgaggcagat gcattccatg
     3241 ctctttcgcc tcgctttggc gttatggcaa cgataattaa cgccaacgtg tcggaatcca
     3301 acgccaaatc cgcgcctttc aatcaatgta tcagtgtggg acataaatca gagatttccg
     3361 cctctattct tcttgcgctg aagagagccg gtgtgaaata tatttctacc cgaagcatcg
     3421 gctgcaatca tatagataca actgctgcta agagaatggg catcactgtc gacaatgtgg
     3481 cgtactcgcc ggatagcgtt gccgattata ctatgatgct aattcttatg gcagtacgca
     3541 acgtaaaatc gattgtgcgc tctgtggaaa aacatgattt caggttggac agcgaccgtg
     3601 gcaaggtact cagcgacatg acagttggtg tggtgggaac gggccagata ggcaaagcgg
     3661 ttattgagcg gctgcgagga tttggatgta aagtgttggc ttatagtcgc agccgaagta
     3721 tagaggtaaa ctatgtaccg tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc
     3781 atgtgccgct caatacggat acgcactata ttatcagcca cgaacaaata cagagaatga
     3841 agcaaggagc atttcttatc aatactgggc gcggtccact tgtagatacc tatgagttgg
     3901 ttaaagcatt agaaaacggg aaactgggcg gtgccgcatt ggatgtattg gaaggagagg
     3961 aagagttttt ctactctgat tgcacccaaa aaccaattga taatcaattt ttacttaaac
     4021 ttcaaagaat gcctaacgtg ataatcacac cgcatacggc ctattatacc gagcaagcgt
     4081 tgcgtgatac cgttgaaaaa accattaaaa actgtttgga ttttgaaagg agacaggagc
     4141 atgaatagaa taaaagttgc aatactgttt gggggttgct cagaggagca tgacgtatcg
     4201 gtaaaatctg caatagagat agccgctaac attaataaag aaaaatacga gccgttatac
     4261 attggaatta cgaaatctgg tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa
     4321 aacgacaatt gctattcagc tgtactctcg ccggataaaa aaatgcacgg attacttgtt
     4381 aaaaagaacc atgaatatga aatcaaccat gttgatgtag cattttcagc tttgcatggc
     4441 aagtcaggtg aagatggatc catacaaggt ctgtttgaat tgtccggtat cccttttgta
     4501 ggctgcgata ttcaaagctc agcaatttgt atggacaaat cgttgacata catcgttgcg
     4561 aaaaatgctg ggatagctac tcccgccttt tgggttatta ataaagatga taggccggtg
     4621 gcagctacgt ttacctatcc tgtttttgtt aagccggcgc gttcaggctc atccttcggt
     4681 gtgaaaaaag tcaatagcgc ggacgaattg gactacgcaa ttgaatcggc aagacaatat
     4741 gacagcaaaa tcttaattga gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg
     4801 ggaaacagtg ccgcgttagt tgttggcgag gtggaccaaa tcaggctgca gtacggaatc
     4861 tttcgtattc atcaggaagt cgagccggaa aaaggctctg aaaacgcagt tataaccgtt
     4921 cccgcagacc tttcagcaga ggagcgagga cggatacagg aaacggcaaa aaaaatatat
     4981 aaagcgctcg gctgtagagg tctagcccgt gtggatatgt ttttacaaga taacggccgc
     5041 attgtactga acgaagtcaa tactctgccc ggtttcacgt catacagtcg ttatccccgt
     5101 atgatggccg ctgcaggtat tgcacttccc gaactgattg accgcttgat cgtattagcg
     5161 ttaaaggggt gataagcatg gaaataggat ttactttttt agatgaaata gtacacggtg
     5221 ttcgttggga cgctaaatat gccacttggg ataatttcac cggaaaaccg gttgacggtt
     5281 atgaagtaaa tcgcattgta gggacatacg agttggctga atcgcttttg aaggcaaaag
     5341 aactggctgc tacccaaggg tacggattgc ttctatggga cggttaccgt cctaagcgtg
     5401 ctgtaaactg ttttatgcaa tgggctgcac agccggaaaa taacctgaca aaggaaagtt
     5461 attatcccaa tattgaccga actgagatga tttcaaaagg atacgtggct tcaaaatcaa
     5521 gccatagccg cggcagtgcc attgatctta cgctttatcg attagacacg ggtgagcttg
     5581 taccaatggg gagccgattt gattttatgg atgaacgctc tcatcatgcg gcaaatggaa
     5641 tatcatgcaa tgaagcgcaa aatcgcagac gtttgcgctc catcatggaa aacagtgggt
     5701 ttgaagcata tagcctcgaa tggtggcact atgtattaag agacgaacca taccccaata
     5761 gctattttga tttccccgtt aaataaactt ttaaccgttg cacggacaaa ctatataagc
     5821 taactctttc ggcaggaaac ccgacgtatg taactggttc ttagggaatt tatatatagt
     5881 agatagtatt gaagatgtaa gg
//
LOCUS       DACDTO010000121         5861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDTO010000121 DACDTO010000000
VERSION     DACDTO010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.124"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..319
                     /locus_tag="IWK70_002650"
     CDS             <1..319
                     /locus_tag="IWK70_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9615143.1"
                     /translation="KECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINAN
                     LNENKIETALNKIQDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQ
                     IAY"
     gene            292..969
                     /locus_tag="IWK70_002651"
     CDS             292..969
                     /locus_tag="IWK70_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9615144.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            959..2101
                     /locus_tag="IWK70_002652"
     CDS             959..2101
                     /locus_tag="IWK70_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9615145.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            2114..3091
                     /locus_tag="IWK70_002653"
     CDS             2114..3091
                     /locus_tag="IWK70_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9615146.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            3091..3999
                     /locus_tag="IWK70_002654"
     CDS             3091..3999
                     /locus_tag="IWK70_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9615147.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            4000..4752
                     /locus_tag="IWK70_002655"
     CDS             4000..4752
                     /locus_tag="IWK70_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9615148.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            4757..5704
                     /locus_tag="IWK70_002656"
     CDS             4757..5704
                     /locus_tag="IWK70_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9615149.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT         2151 a          734 c         1123 g         1853 t
ORIGIN      
        1 aaaagagtgc aaatttatga tcggaaattc ttcttctggg ataattgaag ctgcatcatt
       61 caaaatacca gtaataaatt taggaaccag acaaaatgga cgagaacaat ctaaaaatat
      121 tataaatgca aatctaaatg aaaataaaat tgaaactgct ttgaataaaa tacaagatcc
      181 tcagtttata aaaagcttga aaaatgttaa gaacatctac ggtgacggca acagttctag
      241 aagaataaca aaaacattga atcaaatatt gaatgatggg gaagatatta agtggattca
      301 aaaacaaata gcttattaaa atatactagt gatccataca ttattattgg ggctggcgga
      361 cacgcaaaag tcattattga aaccttaaga ttagaaaata agttaatata tggatttttt
      421 gatgataagc aaaataaaat gcaagatctg aaatatcttg ggaaaataaa cgaattgaat
      481 agctgggcgg atagcaaaaa aaaaatctat aaatttattg tagctattgg tgactgtgta
      541 gtaagaaaaa agattataaa aaaattgaat atgccaagtg agcaatatgg aatagctgtt
      601 catccaagtg ctatagtggc aaaagaaatt tcaattggtt atggaactgt tatttttgcg
      661 aatgctgtaa ttaacccaga tgctgtaatt ggagagcatg ctataataaa cacaggtagc
      721 attgttgagc atgattgtag aattaataac tatgttcatt tatctccagg tgtatgctta
      781 gcgggtggag tacatgtagg tgtaggaact caaataggaa ttggttctca atgtatccag
      841 atgaagaaaa ttggatcttg gtgtatgata ggcgcaggga gcactattgt taaggatatt
      901 ccctcacatt ccttagctta tggcaatcca gctaaaatta agaaagaagg aattaatttt
      961 gaattctaga attttattat caagtcctca tatgggagga acagaagaaa aatatgttgc
     1021 taaggctttt aaagataatt ggatagcgcc attaggtgaa aatgtggata aatttgaaga
     1081 aagtattaca aaatatactg gttctaaggg cggagcttct gcgttaagtt caggtacagc
     1141 tgccatacat cttgccttaa gactgcttaa tgttggaaaa aacgatattg tctttgctcc
     1201 ttcatttact tttatagcta cagtaaatcc tattttatat caaggggctg taccagtgtt
     1261 cattgactca gaacctaata catggaatat gagccctgtt gcactagaga gcgcattaga
     1321 agaagctgaa agaaacaacc aattacctaa ggcaatcatc gtagtgaatt tatatggtca
     1381 aatggctgat tatgaacgaa ttttagctat tgcaaaccaa tttaatgttc cagtgataga
     1441 agatgcagcg gaatcgttag gttctactta taagggaatt tcgtcaggct tatttgggac
     1501 aattgggatc tattcattta acggtaataa gatcatcaca acctcaggtg gaggagctct
     1561 agtgtctagt gataaaaaat atgttgagca ttcaagattt ttagcaacac aagcaagaga
     1621 tcaagcaccg tattatcaac atagcgaaat aggttataat taccgtctaa gcaatatttc
     1681 tgccggtatc ggacgtggac aaatggaagt attgaatgat agagtagcta aacgaagaga
     1741 aattttcaat cgatataagg aagctttttc tggaattgat agaattgaaa tgatgccaga
     1801 agtggatggc atggtgccaa atcgttggtt gtctacttta acgatcaatg attataattc
     1861 tattgatcaa gtagttaacg taataaataa actaaatgaa aaaaacattg aaacaagagc
     1921 tttgtggaaa ccactacatt tgcaaccagt atttcaaggg gtaaaaatgt attaccatga
     1981 taataatctt agtgtgtcag aaaagttatt taatagcggc ttatgcttac cttctggaag
     2041 taatatgaca gatagtgaac aagaatttgt tattgatgag tttaaaaaaa tattcaagta
     2101 ggtgtgatat tttatgaaca taggaattat tggtttaggt tccatgggga aaagaagact
     2161 tagactcatt caagaacact ttaatgatat tttactatgt ggtgttgata ctaatgaggc
     2221 gagacgaaag gaagttgaaa aggaatttgg aataccaact tataatagtc taacagaggc
     2281 aaaaaatttg tttgaaataa attcagtttt tgtttgtaca tcccctataa cccatgaatt
     2341 tattataaaa gaggctttaa atttagattg taatatcttt acggaaatca atttattaaa
     2401 tgaatattat gatgaaataa tcgaagtagc taaaaagaaa gatagattac tttatctatc
     2461 ttctacattt ttgaagagaa aagaaattca atatattaag tctaaagtga cgcataataa
     2521 aaaaatttct tatcaatacc atgtaggaca gtatttaccc gactggcatc cttgggaaga
     2581 ttatcgcaat ttttttgttt caaacaaaaa aactaatggg tgtagagaaa tattcgctat
     2641 tgaattacct tggattatag atacatttgg gaaagttaaa tcgtttactg taatgaccca
     2701 gaaattatcc aatcttgata ttgattattc ggacagtttc actcttatta ttgagcatga
     2761 aactggtata gttggaactt taagtgtaga tgttgtttca agagtagcta aaagaaattt
     2821 aactataatt ggtgaaaaga ttttaataga atgggaaggt aacccggatt ctctattttc
     2881 atggaatgaa gaaacttcta gtatgacaga tattcaattg tacgatgagg ttatgcatga
     2941 taataattat tcacatacta ttattgaaga tgcatattta gaggagatta aagaattttt
     3001 cacgcttttg gaaaagaaaa tggaacccca atatactttt gaaaaagata agtatattat
     3061 taacttaatt aacgacattg aggtaaatta atgatgaaag taattttttt tgggttaggg
     3121 tcaattggaa agagacatct aaaaaatcta ataagctatg gaaaaaagaa ttctattaat
     3181 tttgatataa cagcttatag tagcagtgga aaaaaagagt ttgaagaaat aacgtatatt
     3241 caaaatagag acgatttgga tacacattat gatatagctt ttattacaaa tccgactttt
     3301 aagcatctgg aaacactaaa tgtaattgtt gaaaaagttg attacatatt tttagaaaaa
     3361 cctgcttttt ctgagagtat tgatcttact caatataatt atgattacaa aaatatttat
     3421 gttgctgctc cactgaggta taaaaatatc atggaagttg ttcgtaatag tattcaaagt
     3481 aaaaaagtat attctgtaag agctatttgt tctacttatc ttcctgattg gagaaaaggt
     3541 actgattata gaaataatta ttctgccttt aaatctatgg ggggaggagt agaattagac
     3601 tgtatacatg aattggatta tattataaac ctatttggtt tccctaaatc agtaaaaaaa
     3661 cagtttggta aatattcgaa tcttgaaatt aacagtaatg atttagcaac atatatattg
     3721 agatacgatg ataaaattat agaaattcat ttagattatt ttggaagagt tcctacaaga
     3781 aaatttcaat tgtttacttt agatgaatgt atcaatgttg acttattaaa taataaaata
     3841 agttttgaag aaaacattgt taaagaaatg cctgaatctg ctaatgatat gtatgagaag
     3901 gagttgaaat atttctttga aaacgtagta acaggcaaag aaaactggaa tgatttatat
     3961 catgcaaacg aagtattgaa aatagctgag gagaaataaa tggaaatttt atttactatt
     4021 tgtggtagag ctgggtctaa ggggtttaaa aataaaaatt tgaaatattt ggcaggaaag
     4081 cccctagtcc atttcgcttt agcaatgata gatatctatc aacaaaaaca tcctgaacat
     4141 aattgcacga cttgtttgaa tacggacagt aatgagttag tagaattgac gactaagtta
     4201 aatccgtctg tgaaaattgt agaacgtaaa cctgaattag ctacagatac tgtagggaaa
     4261 attgaagtga tacgtgattc attcttacaa ctagaaagag aagataatat atttgattta
     4321 attatagatt tagatttgac ttcaccttta cgtgaagttt ctaatcttga agaattaata
     4381 gatgaacatc taaaaaattt agataaagac gtctactttt cagttgtttc tgcaagacgt
     4441 aatccctatt ttaatatggt aaaaagaatt gaaaatgaag ttactatagt taataaatca
     4501 agatacacat ctaggcaaca gacacctgaa gtttttgaaa tgaatgcctc aatgtattct
     4561 tttaaacctg aatttctaaa aaaaagtaat tatatttttg atgggcaatg tgggataata
     4621 aaaatgagag attatctaat tttagatatt gatagtgaag aagattttaa atggttagaa
     4681 tttttgtttc ctaaattttt agaagattct tcggagcttc aagatatcta taattattta
     4741 tatcggaggt aaaaggatga gctgttatct tatcaccggt ggggctggct tcattggttc
     4801 taccttagcc aatcatttag gagaagaaca tgtggtaatc gtcgtcgatg acttatcaat
     4861 gggaaaagta gaaaatcttg atatgacgaa aaatatcact tttattgagg gagatgtcgc
     4921 tgagaaatcc ttgatggaag agatcatgaa agctaataaa tttgattata tctttcattt
     4981 agcagcagtt gctagtgtgg cagattcagt tgcccgacca gtagaaacac atcgtgtgaa
     5041 ttttgaaagt gtattaatga ttttagaatt gatccgcaag tatcaacctg atttaaaacg
     5101 gatcgtgttt agttcttcag cggctgttta tggggatgaa ccgactttac caaaaaaaga
     5161 agagtccgtg attcgcccat tgacgcctta tgcggttgat aaattcgctg cagaacaata
     5221 tgtgctagat tattgtcatt tatatgatgt acccggtagc gcagttcgtt ttttcaatgt
     5281 ctatggtcca aatcaaaatc cgaattctcc atacagtggt gtgatttcta ttctcgtaga
     5341 tcgctataag aaacaaatcg taggagaagc aacttcattt acattatttg gtgatggaag
     5401 ccagtctaga gactttgttt atatagatga tgtaatccaa gcattacttt tagttgcgaa
     5461 tgaggaagct gcactgggga aacaattcaa tgttggaacg ggtaaagcaa ccacactttt
     5521 ggaattgatt catgcaatca atgaaattct cggtgtagaa ttgacacttg aatacaaaga
     5581 agagcgttcg ggtgatatcc atgactctct agcagatatt actaagatcc agtcaattgg
     5641 ctatcaaccg aaatatggta ttttaagtgg aatgactaat tatttgaaaa ctgaaatcaa
     5701 ttaaaacata ctaagataag attagagggt aaattcaggc tctttgtcaa taaggactga
     5761 tgagttttgt aaaatcaaat ctgggataga agggctcttt gtcaaatgat gttggtaaag
     5821 agaaatctta gaataatttg gacagaacag aaagaagaaa a
//
LOCUS       DACDTO010000122         5809 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.125, whole genome shotgun sequence.
ACCESSION   DACDTO010000122 DACDTO010000000
VERSION     DACDTO010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.125"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            450..1604
                     /locus_tag="IWK70_002657"
     CDS             450..1604
                     /locus_tag="IWK70_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP9615150.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="IWK70_002658"
     CDS             1598..2020
                     /locus_tag="IWK70_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615151.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="IWK70_002659"
     CDS             3143..4075
                     /locus_tag="IWK70_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9615152.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="IWK70_002660"
     CDS             4092..4607
                     /locus_tag="IWK70_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9615153.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="IWK70_002661"
     CDS             4903..5184
                     /locus_tag="IWK70_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615154.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="IWK70_002662"
     CDS             5186..5602
                     /locus_tag="IWK70_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615155.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2227 a          961 c          931 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtgtacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DACDTO010000123         5790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDTO010000123 DACDTO010000000
VERSION     DACDTO010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.126"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWK70_002663"
     CDS             complement(99..1229)
                     /locus_tag="IWK70_002663"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9615156.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1877..3079)
                     /locus_tag="IWK70_002664"
     CDS             complement(1877..3079)
                     /locus_tag="IWK70_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9615157.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="IWK70_002665"
     CDS             complement(3139..3342)
                     /locus_tag="IWK70_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9615158.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="IWK70_002666"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="IWK70_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4879..5691
                     /locus_tag="IWK70_002667"
     CDS             4879..5691
                     /locus_tag="IWK70_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9615159.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DACDTO010000124         5617 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDTO010000124 DACDTO010000000
VERSION     DACDTO010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.127"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..301
                     /locus_tag="IWK70_002668"
     CDS             <1..301
                     /locus_tag="IWK70_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9615160.1"
                     /translation="ISLKDICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSY
                     KAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            312..2516
                     /gene="topB"
                     /locus_tag="IWK70_002669"
     CDS             312..2516
                     /gene="topB"
                     /locus_tag="IWK70_002669"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9615161.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            2762..3355
                     /locus_tag="IWK70_002670"
     CDS             2762..3355
                     /locus_tag="IWK70_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9615162.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            3371..4270
                     /locus_tag="IWK70_002671"
     CDS             3371..4270
                     /locus_tag="IWK70_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9615163.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            4273..4758
                     /gene="ssb"
                     /locus_tag="IWK70_002672"
     CDS             4273..4758
                     /gene="ssb"
                     /locus_tag="IWK70_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9615164.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5124..5384
                     /locus_tag="IWK70_002673"
     CDS             5124..5384
                     /locus_tag="IWK70_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615165.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2053 a          978 c         1144 g         1442 t
ORIGIN      
        1 cattagtttg aaagatattt gtagagaagt cgcaaaagaa ttaaaagatt gtgaacgctc
       61 gaatggtacg tatgattttg atcgttttta tcaaggaaaa ggggatatta attctcagat
      121 tcttttctct tataaagcag aacaatacta caaaggctat aaaaatcatg aacatgaatt
      181 tgtacctgaa gtgaaaaagg attggaacga tcaattgaag catgagatcc atcaacagga
      241 aattagaaag caaaaacgtg cgatgttgtt tcaacaaggc agacaacaag aaagggagtg
      301 agatcgtcac gatgaaaact gtcattttag cagaaaaacc gtcacaagcg aaggcctatg
      361 ccgatagctt ttctaaagcc acccgaaaag acgggtattt tgaaatccaa gatcgattgt
      421 tttcgggtga aactgtgatc acgtatggtt ttggtcattt agtcgagtta gattcgcccg
      481 atatgtacga tgaaaattgg aaacaatggt cattagagca tttgcctatt ttccctacac
      541 attatcacta tcatgttcct aaagataaga aaaagcaatt caacgtggtg aaacagcaac
      601 ttcaatcggc cgacaccatt attattgcga cagatagcga tcgtgaaggg gaactgatcg
      661 cttggacgat cattcaacaa gcaggcgctg atcaaggaaa aacctttaaa agactttgga
      721 tcaactcctt agaaaaagaa gccatttatc agggattcca gcaattacga gacgcagaag
      781 agacctatcc caaatttgaa gaagcacaag cacgccagat cgctgattgg ttgatcggca
      841 tgaacggcag cccactgtat agtttactct tgcagcaaaa gggcatacca ggaagttttt
      901 ccttaggcag agtccaaacc ccaacgcttt acatgattta tcagctgcag gaaaagatta
      961 gaaacttcca aaaagagcct tattttgaag ggaaagccca agtaaccgct caaaatggca
     1021 tatttgatgc gaagcttgat cccaatgaaa cccaagcgac gcaagaagca tttgaagcct
     1081 atttaaaaga aaaaggtgtt caagtaggaa agcagccagg cacgattctt gaagtcgaaa
     1141 ccgagaaaaa aagcgcggca agtccacgtc ttttttctct atccagcctt cagtctaaaa
     1201 tgaatcagct catgaaagct agtgccaaag atactttgga agccatgcaa gggttgtatg
     1261 aagggaaata tctaagctat ccacgaacag acacccctta tattacagaa ggagagcatg
     1321 cgtatctgtt ggatcattta gatgaataca agcatttttt aaaggctgaa tcattcccta
     1381 ctcctattca tacaccgaat agtcggtatg tgaataataa gaaagtccag gaacattacg
     1441 cgatcattcc tacaaaaaca gttatgacag aagcaacgta tgaaaagctg acacctttac
     1501 aacaagcgat ttatgatcaa gttttaaaga cgacggtggc catgtttgcg gaaaaatata
     1561 cctatgaaga aactacgatt ttgacacaag tccagcagct tcagctaaag gcagtcggaa
     1621 aagtaccagt agatctaggt tggaaaaagc tatttggcaa agagagcgac agaaaagaga
     1681 aggaagaaga accgctactt cctaaagtaa caaaaggaga aacggtgacg gtagatctac
     1741 aaatctttga aaaagaaacc aagccaccac aaccttacac ggaaggtaca ctaatcaccg
     1801 ccatgaagac agctggtaaa accgtggata gtgaagaagc acaatccata ttgaaagaag
     1861 tggagggaat tggtacagaa gcgacacgag caaatattat tgaaaccttg aagcaaaagg
     1921 aatatatcaa ggtagagaaa aataaacttg tggtgacgaa taaaggaatt ttgctttgtc
     1981 aggctgttga aaaagaaccg ctgttaacaa gtgccgaaat gaccgccaaa tgggaaagct
     2041 atcttacaaa gattggtgag caaaaaggga cacaagctac ttttctagca aatatccaaa
     2101 aatttgtttc ccacttgttg gaagtcgtcc caggacaaat ccaagccact gattttggag
     2161 caacgttaca agaggtgaaa gtagccagtg aaaagcaaga agcggcgcgt catttaggat
     2221 cttgtccaaa gtgccaggaa aaagaagtca tactttatca gaaggtggcg acttgcacgt
     2281 ctgaagcctg tgattttaaa ttgtgggtaa cgattgccaa aaagaaactc acagcgacac
     2341 aactgaaaga gatcattcag aacggccgaa caagtcaacc agtcagagga ctaaaaggtc
     2401 agaaaggttc gtttgaagca acgatcgttt taaaagaaga ctttacaaca ggatttgagt
     2461 tttctgaaaa gaagaagacc acctacaaaa gaaaagtgag acgtaaaacg agttagaatt
     2521 gacggaggga aaaacgatga gaaatacaac gcataaagaa caagaaatcg aaaatcaaaa
     2581 agccaaaata cgttcagagc aggagaaaat gcgtgccagc aaacgaagac tacagcaatt
     2641 gaagaatcaa caacggattc aaaaagaact tgaattagat cgataaatcc tcctgttgtt
     2701 aatattaaac taacatgtta tagttatgtt agtttaatat taacattcga gagggaaagc
     2761 gatgaaagat aaagtcaagt gcatttttga gaaatacaat ggtaatctat ccttgaagga
     2821 tgctagaaat gaagggttat cacctatgac gttagatcgt ttagccgaaa aggacaaaat
     2881 agaaaaaata gcatcaggtt tttatacctt aaaagatagt tttgtggatg aattatatct
     2941 cgctcaaagt atctatacta gaggaatctt ctctcacgag acagcgcttg atctttttca
     3001 agtaggaaca tatatcccta aaaaaattaa tatgatgttt cctaaaggat acaatgtttc
     3061 gaaagagaat ttagatcagt atgccattca accgcattac acgaataaag aaaactttga
     3121 actgggaatc acagaggcag aaagctttta cggcaataaa attttcgtct atgatttaga
     3181 gcgaacgtta tgtgatatgt ggaatcccag atataaagca agcgtagaag taaaacaaga
     3241 agcattaaaa gaatatatga caagtaccag cagaaataca aataagctaa gaagatacat
     3301 gaatatttta aaatcaccaa aagaaatgac tttgtatatg ttgccacttt attgagagga
     3361 gtaaaggtga atgacaccga cacaatttaa agcgaaaaca agaaatgttg cagctgagaa
     3421 acatttaaac ccacaaattg ttcaaagata ttatatgatg gaaaaattat tagaatgtat
     3481 tgttgaatcg ccttatcatg atgattttgt attaaaaggg ggatttctga tcggatcaaa
     3541 atacgggtta gaaaaccgtt caaccgttga tattgatacg acttatcgaa attcgaaact
     3601 aacaaaagaa aaattagaaa ctgttttttc tgaattaaca gctgctccta caaaagatgg
     3661 cattcggttt gaattaaagg gcttaacaga gacgagggaa gccgattatt acccaggttt
     3721 tcaagcaaag tttattgctt ttcttgaaaa tgcacgtgta ccttttaaat tagatattac
     3781 ttcaggtgat tctattattc cagcagcatt gatatatgaa cataaattga tgtttgaaga
     3841 taaaacaatt catattccag cttatcctac ggaacaaatt ttagctgaaa aattacatgc
     3901 cacgtttagt ttcggaatag ataattcacg tatgaaagat ttctatgatc tgtatatgat
     3961 ccctaaacta gaacgtatcg atgagataaa tttgtataag agtgttcaag gaacatttaa
     4021 ggaaagaaat aatcaatcag tgtttaagaa tttgtataat caggaaatgc ctcaattgat
     4081 gaagaatgag ggattgaggg agaaatggaa aagatatcag caagaaaact attttgccaa
     4141 agatatttct ttcgaatcta ccttaacttc tgttgattcg ctgatgcaat caattgtcga
     4201 gcaagaggaa gtagaacgaa aacaaaattt taagagatca caacaaatga atcaagaaat
     4261 ggagagataa agatgatcaa taatgtaact ttagtaggtc gattaaccaa agatcccgat
     4321 ttacgttaca ccgcaagtgg cactgcagtg accactttta ccttagccgt taaccgtaat
     4381 tttactaatc aaagtggcaa tcgagaagct gactttatca attgtgtgat ttggcgcaaa
     4441 ccagctgaaa cgatagcgac attagctaaa aaaggcagct tgatcggcgt cgtcggacgg
     4501 attcaaacgc ggacttatga caaccagcaa ggacagcgtg tctatgtgac tgaagttgtg
     4561 gccgataatt tccaactatt agaaagtaaa gcagcgacag aaagccgtat gcacgctgat
     4621 caatcgtcca gcagtccaag ccctaccacc tttgaacaac gggatacagc gacgcctaac
     4681 aacaatggat tgaacgcttc acagaatcca tttggtggac aatcaatcga tattagcgat
     4741 gatgatctac cgttctaagt agcattttct attggttgca tttgctacgg tttatgttat
     4801 aatttagaca acaaaaagaa ttttcaatct ataaccaaaa accactagct ggcgggctag
     4861 tggctttctt ttacgtttct attataagaa ttaacgggta ggcttactgt ctttcttggc
     4921 aaatttacaa tcaagccatt tgcagatata gtgcgcggct acttctgcca tgattgcaaa
     4981 aacaaaatca aaaagaattt tcaaacctat aacctcctcc ccttgccaga ttaggccggg
     5041 gtagcagaca gcaagactat tatagcataa ttgaaatatc caaatgattg gatttttgct
     5101 ataattttag cgaggtgatt gtgatgaaca aatatgttgg tttgcttgat aaaatccgag
     5161 taataaaaac gcaaccgtta ttggttcgct ttactttaca aacgattcat gaatcgatta
     5221 attgtgtagt tgcagatatt gaaattatcg ataaattgct aattatggat gatggaaaat
     5281 acaatattgc cgtcacaggt cacttcaata aacgaaatca gctagttatt gctagcatgt
     5341 atgttagaaa tcccgatcat tttacgcgat cgatgggcat ataaataaaa gaagagttgt
     5401 agtcctaaat gaggattgca gctctttttg ttttacatag aaacaggaaa agaccttttt
     5461 aggaagacac aagtgccttc tcaattgaaa aacctttctc tcactccaac cgccaccgcc
     5521 gttcgagaag aaaggaagtg aacgctgcac gttcactaag atatggtgtc ttgcaaagca
     5581 agacaaaata gacaaaactt cctctcacca aaaggtt
//
LOCUS       DACDTO010000125         5612 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDTO010000125 DACDTO010000000
VERSION     DACDTO010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.128"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(59..2008)
                     /locus_tag="IWK70_002674"
     CDS             complement(59..2008)
                     /locus_tag="IWK70_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9615166.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(2050..3108)
                     /locus_tag="IWK70_002675"
     CDS             complement(2050..3108)
                     /locus_tag="IWK70_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9615167.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(3227..3682)
                     /locus_tag="IWK70_002676"
     CDS             complement(3227..3682)
                     /locus_tag="IWK70_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615168.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(4266..5069)
                     /locus_tag="IWK70_002677"
     CDS             complement(4266..5069)
                     /locus_tag="IWK70_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9615169.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1653 a         1108 c          777 g         2074 t
ORIGIN      
        1 cttacgaccc atcgttttat tctaaggttt tgtagtaaat atttcttgaa tttcatagct
       61 acgcccctcc aatcgaaaat acaatcttgt tttctttact ttcagcaacg gaaggctttg
      121 caataatttc agcattagaa tcaccttttc cttcaaacgc tccggcttca ctcaacttaa
      181 catcgaatat ttttgcgttt ggataagtaa ttattgcttc agtaatcgct tcttcaaact
      241 cagattcaac taaatgcaag atattccttg caccccctga tttagaatcc tcttgtgaag
      301 ccatgtcaaa aactaagtgc atggagagat cgtctgcata aaaattttga ccatatactc
      361 tttctttcct aggtggaaga cgtaaatcta tatttaaatg ttttcgtact aattcagcac
      421 tcttcaacat ttcattataa gcaatttcca gaagagtttg ttcggataaa gcccaaaatg
      481 gaactattcg tccaaatcgg ttaataactt caggctttag accttcacca atcaaggctt
      541 gtttgatccc tgatgcgact tttcggtatt ctctttttag ttcagtgctg tattcattag
      601 aattttcctt gtttgtaaca cgagatagtg agctatatcg gtttaacgaa tcgaacatct
      661 ccgcacctgc atttgttgtc gcaaataaaa ttgtatttga caatgaaacg gtgtcgtgaa
      721 cttctcgatc atcccatcct ctttcagtaa aacttatcct accttcatct aaaattgata
      781 aaaaggcatc gagtgtttct acaccagctt tctcatattc atcacacaca ataatcgcag
      841 aaggatcatg ccttacatga gtacctataa gtcgtctaaa acgcattgca tcatctttgg
      901 ttttgtagtc cggcatatta actaagagaa ctttattttc atcgccataa agtcctcttg
      961 ccacggcttt agccattgca gttttaccga cccctgttga tcctgtaaac agagcagttg
     1021 cttttggttg ctttcgacct ttaaaacgat caaatccaag cttcatacgt ttaaacaaca
     1081 ttgttatagt ataaactgct aacggttgtc cttttatgga ctttaattcc tcttgaagtt
     1141 tttccatatc aaaatcagaa tctagctgaa tattatactt accaaatatt tgcaatagac
     1201 tttctcgatt ataagcattt ccgtctactc gatcttttgc aatcaaatcc tccattacag
     1261 caattgattt agccggttcg gacaaatcct cacgataaag tttgtttgct tgtacgattt
     1321 cttgaagtaa ttcatcagga atcctttgcg ataatctaaa agaagcctga ggatcatctt
     1381 taacagagga tgtatttagc cattgtctca aaatatcgac gacaacatcg ctttttagtt
     1441 cattcaattc taacggttgt agccgtctcc ctaaggcatc atctcttttg atatagtttt
     1501 catattcttt tcttgtagta gcggtaatca tttgaagtga ttcatttgct aacggttctt
     1561 ttagcgaatc acccccaatt ttattgccac tcccaaaaga gctaacaact ttatgaaact
     1621 cgtcaatgaa gaggacaaat tcattatctt tgtcctcagc ttttaaaatt cgctcatatt
     1681 ctttcaatct tggaattaat gtttctaaac gtgtttggag gacatcatct cccccaacac
     1741 gcaattgtcc gattgataat ttatatactt gtactttctt tccatcttgt cgctgtcttt
     1801 cagcccattc acttgcaagt gctgttttac ctgttccggc ttcacctaac aagatagctt
     1861 gcggtgtttc cggtctattc aagatcagtg acaactcatg gagttcgtca tcacgtccca
     1921 ctaacttatg ttttggtggt ttcattggtg tgagagcatg attaaattcg gtttgaatat
     1981 tcttaaaaaa ctcggtacta ttttccatct gtttgtgttc acttcctcta aaataagaat
     2041 aatttacttc tagtcagtca aagttaggta cttaaatgtc gtcttcatgt ctccaaaaga
     2101 atctttgtta aagtcagcat ttttgtattg atcaatcaat ttttgtgtat cattgattgc
     2161 tttaatttcg gtctccattc gactgtctag ttgacgaata ctttcttgcg aatctttatc
     2221 gtcaggattt tgttgtaaac gttccagata ctcagctttt gtggcttcaa ttttcttgat
     2281 gttagtattt gaagcattta actgatcttc tagcttattt acaacattat tcttacttgt
     2341 aactgctaca acttttgaat aatcaatatc gccatctttg gtaagaaagc ttccttgata
     2401 gacattagtt gaatctgaat atggatttat tctgaaattg atattatcag gagtattaac
     2461 aggttttgct tttttcccaa aagaaattac tccttcatta ttaacatcat tcatagaata
     2521 ttgagaaagt gcttttgtaa ctgaatctgc ttcattcaca ttgactttgg tatcatcgtc
     2581 agctgtatca tcaccagaaa ttagtgatac ttggtttgca ataaaaactt gataagctct
     2641 ttcctctaat ttacctttta ggaataagaa agcatcccca tcagtactta acatcccata
     2701 gctcatttca agcttcttag gacgatcttt ttcttttgtt gccatatata agttatagtt
     2761 ttttccttgc gtagataaca actttctagc gttttgggag taaccaagtt ttactactgt
     2821 tacatctcta ttttgatctg tccacacatc tgaaatagtg atttctgctc ctgatcgagc
     2881 aaactccaat gaagacccta taggtgttgt attgttcacg agataataat tatagtttcc
     2941 taattgataa atgtagctac ccaagcctgt taataaaaaa agcactccta gaaaagttgc
     3001 aagcataatg cgtaatccgt gacgtgttct cttatcctta tcaccaagca accctccaat
     3061 aatactgtat tgatctttgg tagatgttga attgacgttt ttttccatca gtttgtctgc
     3121 cccctttcat aaaagagcaa acaatcagac gttttttcgg taattctagg ttcgggattt
     3181 tgaatcattt cggtatattc ttccccatta atatttaaca caaaaattat cttcctttct
     3241 tcgttccttt cttttgctct ttattttcca aataatcaat gatttttgct attgtaccaa
     3301 gatagtaatt cccctcagct ttgttttttc ggattttgct aaccattgga gtaggtacaa
     3361 tctcttcatc tctcaattca ctgaaagatt ttttacctag atattttcca ataacagatt
     3421 ttaaaaaacg ttcaaaatct ttctcgtaag ttgttttctt gccttttagt tcatggttca
     3481 tattgtcaac gacttttgcg gtttcaataa ttgttttagt tctcagctta ataatttcat
     3541 ctggaatttt caatcctcgt aatttcatcg ttgtactaac tggtaaccca tgctttactg
     3601 catcagcaat actaaaactt tttagatcat ctagtaagct ctccatatag ttaacaaatt
     3661 catgccaatt aatagcattc attgtctcac cccttcattt ttttaaaatg aggttcgggc
     3721 gtaacacatt tatgttatta taatatcata ataacataaa taaatcaatc agcaaattta
     3781 gatatccctt aaagtatcag caattattca acaagatatt tattttagct atttcagtca
     3841 tcataaaaga ctaattagca ataaaataac taatattatc catttatcag acgaaaagaa
     3901 aaaacttatc taattactct atattaactt ttaacaaata gttgacttgt ccaaaaatgt
     3961 taccttcaat ctcacaaaga tattaagatg tttcctacca aaactgatat caccggactt
     4021 tttattctgt gttgtccaaa agtgttacct ttagtcgatc aacaaattaa aaaaaattgt
     4081 ttttttcttg acttgtccaa aagtgttacc catagttgtt aaaatttgta tagattagcc
     4141 attctttcga atattattta gactgaaact tgcttacaaa aaaacttctt gcatttattg
     4201 tccaaaagtg ttacccatac ttttttaaag aagcaggttg tattaaaatt acaacctgct
     4261 tcttctcaat cttcttctaa ccaattatgc attgaaatgg ttggcaagtt tgattctggc
     4321 tcactaaaag tgaatctgag ccgatcaact cttcttcctt tctttgcata gattttttca
     4381 taatcaaaag tttcaaatac ctgtattccg ttttcatcag gagctaaaaa ttcttctttc
     4441 gcctgctcta gtattctttt gtcaatattt gtcattctat aacttgaagg tatacttaat
     4501 gctcttctaa aatcttcaac tttgactacc caataaccat ttcgccactc tttatttctg
     4561 tattgcatga gatgacgata caattctttt gtgtagctac ttcttagttt tgtaaaatta
     4621 tcaagttcaa aacgagtaaa gtttttgcca attgagttta agataaattc aaattcctca
     4681 tttgccttaa ttgtaactgt ttccttctgt ttatctatcc ggaatttttg aaaaagccac
     4741 agctgttcat agacgtctga atcttcaaaa cggtaggtta aagtacctag tttgtcactc
     4801 aaagctttta acacagaata aaaactgtcc ttttctcttc tgtcgtattt gaccaaataa
     4861 cgaaagaggt tgaaatcaaa agttacttct gctgttcctt gacgtttcat ttttgataaa
     4921 atagcccaaa acacatccat ttcaacaggc ttaaatcctc gcaaagctgt tgtattcaga
     4981 ccattgttat aacgcacaat tgcatttgtt tgttctgata ctaaatctac atcactatca
     5041 ataatctttt cggtaacttc ttgagacatt gattactctc caatcttctt tttgcgctcc
     5101 tttaatcatg ttaatctggt actaattaaa ggaaacaatt ttttctgctt gttacctaaa
     5161 ttaaatcaca actaaaggta acagtcaaca aagatttgtt actaatctaa ttttctattt
     5221 tttcgccttc caaatcaaga aaaccgaagg taacgtctta tcctagtgtg atacctcaaa
     5281 tgtccacttt tgttaccttt gctgtccaaa agtgttaccc ttactgtcca cttttgttac
     5341 ctttactgtc caaaagtgtt acctttatac ccctttaata cttactctcc caagagatta
     5401 agcctcccct aacagatata ttaacagata tttaacagat atattaacag atatttaata
     5461 aaggaggatt actcctctct tatttcaaaa tcttaaaaat aaaaaatctt aaaaaaaact
     5521 aacactatct aggagtttat tttgcttgtg taactttgaa taattgaaaa aaaggaaaag
     5581 gaggaagctt cagcttcccc ttttatttat at
//
LOCUS       DACDTO010000126         5584 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDTO010000126 DACDTO010000000
VERSION     DACDTO010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.129"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            369..557
                     /locus_tag="IWK70_002678"
     CDS             369..557
                     /locus_tag="IWK70_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615170.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            547..807
                     /locus_tag="IWK70_002679"
     CDS             547..807
                     /locus_tag="IWK70_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615171.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            848..1075
                     /locus_tag="IWK70_002680"
     CDS             848..1075
                     /locus_tag="IWK70_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615172.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1140..1712
                     /locus_tag="IWK70_002681"
     CDS             1140..1712
                     /locus_tag="IWK70_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615173.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1713..2024
                     /locus_tag="IWK70_002682"
     CDS             1713..2024
                     /locus_tag="IWK70_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9615174.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2015..2275
                     /locus_tag="IWK70_002683"
     CDS             2015..2275
                     /locus_tag="IWK70_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615175.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2436..2795
                     /locus_tag="IWK70_002684"
     CDS             2436..2795
                     /locus_tag="IWK70_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615176.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2792..4435
                     /locus_tag="IWK70_002685"
     CDS             2792..4435
                     /locus_tag="IWK70_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9615177.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4470..5552
                     /locus_tag="IWK70_002686"
     CDS             4470..5552
                     /locus_tag="IWK70_002686"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615178.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
BASE COUNT         1998 a          763 c         1152 g         1671 t
ORIGIN      
        1 tccttctaaa ttgttcttat tatctatcat cagttgctgt ctattgtcat tatgtcactg
       61 tggtggaaag gtgtatcgct catctaaaat ttaggtgcaa actgcaaggt tcgattcctt
      121 gccagtgact ttggtaaccg aggcatcggc ggtttaaaaa tataggggtg cgcaatttcg
      181 tacgcgtttt gtgcatcttg taggttgcta ttacataatt ggttgggtta gattgagttt
      241 tgggattcgg tacaaatgaa tcgtcaaatg actcaagcac aggatcggaa acgtccctgc
      301 ctgtgcatta catattagat cactctttga gtggtctttt tattttaaaa aagagaggat
      361 tttgaaaaat ggaattgatt gtatttacta ataacggaca aacatatcat ttttatgaag
      421 tggagaattt taagccaaca acaacaggat ttagttttat ttatactggg aaggctacgg
      481 gagttactcg ttcagctacg tttaacaata catcaacagc tggttatgcg cttgcggagg
      541 tacaaaatga atcatgagaa gtttatcgaa aaatgcaagg ctattgtacg tgaaagaatt
      601 gaaaatgaga ttgctgacct aagtggagca gtgcctgagt ttagtatttt catagtctgg
      661 tcatgcaaaa cactgcaaaa tagcaaagca ttagtcagcg ctagcttaaa aggagcaccg
      721 tattttgaaa ttacgttgaa cggggacaaa ggtgaaatct atgtagatac ttatctcaaa
      781 aaatcaaatg aatgtatcaa agtctagcag gtgctaggct ttttctttac ataaaggagg
      841 ctgcataatg agaaactact ggtatgtatc actaactaat gaatatcctc gaaccattgg
      901 tgattgttca gtgcgtgttg tgcgttctgt acaaatcaaa gggaagtact ctattgtcga
      961 aatgctaaga gaagctacac caaacgaagt ggataaatgc aagctgatat attgcggtca
     1021 tggctattgg aaagacgagt atatccaata caacattgag aggtacatca aatgaatgat
     1081 aactatgatt acatcaagtt gttagcaagt aattattaga tgtttttagg aggaaaacta
     1141 tgaaattaaa agattatatc agagaagggt ataacgttgt aactgcaccc aatctagctt
     1201 acaaaattca agaagattat cccaatgctt tagtgtttac tgatagagct ttagatgcca
     1261 ttcctatagg gaagttatta gtagatcgtt tttatagcaa taatccagct gttcttagtg
     1321 ctaagccact tcaaaacgct tatgctattg aacgctttac ttgcgagttt actggatatg
     1381 agacggtagt accaatgaca ggtgagacag gaagaaaagt aattcaacag atcaaaaaga
     1441 ggattgctga attgacgtta gatgatggaa gtaatacgaa ccttctagaa atcaaactaa
     1501 gagatactga ctcagtacca gaagtttatt ataaaggtca acgaatggat gagatgccga
     1561 taggtcttgt cgatattact tatcattggg atacggaaga atatgaatgc caagtaccaa
     1621 aaggagctaa cgacatcact attgaatatt atgattcaca taataacaag tacttagata
     1681 gaaaaatcat tgggcacaag agagatatgt aaatgattga tgtttcaagt aaacaagcaa
     1741 gagctaggtt ctatggatca actgagtgga ggaaacttag aagtcaatgc ttagagcgag
     1801 atcattatga gtgccaatgg tgtaaagcag agggtagact aacaactcaa catgattcaa
     1861 ttcttgaagt ggatcatata aaggagctgg agtatcatcc ggatttagct ctagatataa
     1921 acaacctaag aaccttatgc aaagattgcc ataacaaaag gcacaaccgt atgaaatatc
     1981 gtggccaaca aaagaaaaga aagtgggatg atgaatggtg gtaagaaaaa cagcaaacag
     2041 agatgatgtc aaacgagcag aagcaatcgc tatggttgat aaggctattg ataagttggt
     2101 tctatttaag agtcagctca ttgaaggaac aacaactgta gaggatatat ctatcaacaa
     2161 tagactaata ggtaacgcta atgaagtaac agggacattt aatattaatg tagacattga
     2221 ttataccttg agtccatttg cacatattgt agaaagcaac gagcaagacg gctgataaat
     2281 cagtataaag cgaagatata tagagtgaaa tacccccggt caaaagattt tgagaagaaa
     2341 taaaattttt gggaaccggt ggatggggtc aactctgcaa ataaatgatg ctattttcac
     2401 acgaccccct ccccacgtag aaaggaagtg agttgatggc tgatctgcaa gagagaaata
     2461 ggcttgtttc tgctgaagaa aggcgtctaa ataaattgtt taaggatatt gataaggata
     2521 aaaagaaggt cgtttctggt ttgattacgc aagcggcacg cttaaaaatt cttcttgatg
     2581 aaatgtggat tgatatttcc gaaaaaggtg attatgagct tttctctcag tcagaaaatc
     2641 aaaaacctta cgagcgggaa cgaccagttg ctaaacagta taattcgaga gaccaagcat
     2701 atcaacgtgt aattaagcaa cttactgact atttgccaga agaaaagcgc gaacctacaa
     2761 aaaatgcagc gctagatggt agtgatttgc tgtgacaaca ttaaagccat tctattttga
     2821 tgaatacgta gatttatacg aaaaaggggt aatccctttt aataaagaaa gaattcagtt
     2881 aattacttat ttaaaaaacg aagtgctagt tcgggatgat atctattttg atgaagaaat
     2941 gattcaaaag tttatcaagt acactgaaaa aaattttttt cctttagcta aatatcaaaa
     3001 atttatcaca cctttcattt tctgctatca aaaagaggac gacgaggtgt tttttgatga
     3061 aattctaaat agtattgcgc gaggcggcgg gaaaaatggg ttcatgtcag caagagactc
     3121 attttttatt agccctctct acggtgtcag aaattatgat gtaacgatta cagcgaattc
     3181 tgagaagcag ggaaaggtat cttttaaaga agtttatgaa atggtacaaa tgcgcaaact
     3241 ggaaaaacaa ttttatttga ctaagatggc tatagtcaac aatgtaacta attcgatttt
     3301 tagttatagg acaaacaatc ctaagactat ggatagtgca agagatggct gcttagaatt
     3361 tgatgaaatc catcagtttg aatcttcaga cctagttgat attcagcgta gtgggctagg
     3421 gaaaataaaa catcctcgaa ctttttataa tggtacaaat ggtcatgtta gagaaggttt
     3481 ttatgacaaa atgttggaac gagcacaaaa gatttttagt ggagagaaca agaatgatcg
     3541 cttgtttccc tttatttgta aactggataa cattgatgaa atggataatc ctaaaatgtg
     3601 gtcaaaggct aatccaatgt tcgaagaaga ttcagcatat tcaaaacgtc tgttttctac
     3661 agtgatgaaa gaatataaaa aactagaaga tgagccatca ggtaggcgtg aatttgttgt
     3721 taaacgaatg aattttacag aaggtgatgc agaatcagat attacgactc atgaaaaatt
     3781 aatggctact aaacaagaat tgccaaattt gaatggtcgt tcttgtgtgg ctggatttga
     3841 ttatgcaagt attcgtgatt ttgcaacagt gggtttgctt tttaaaattg atgataaatt
     3901 tatctggcag caacatagtt ttgctaggaa aaagtttcta gatacattca aactgaaagc
     3961 gcctattaag gattgggaga aaaaaggttt agtgacaatc gttgatgagc catcaattga
     4021 tcctcgtcat ttaattgatt ggttaaatga aaaaagaaaa atccatcaaa tagaaatcgt
     4081 ttgtgccgac ggatttcgta tggatttgct gaaacctctt ctagagaaag aatcatataa
     4141 ttttgagttt atcagaaata taagaggtgt gcagtcaaaa gttgctccta ttattgaaga
     4201 tggttttgca aatgaacggt ttatttttgg tgatgatcct atgatgcgtt ggtatacaaa
     4261 taattcgttt atcaaagaag acaaattagg gaataaaaca tttttgaaaa aagagccagt
     4321 aaggcgtaaa acagatgggt ttcatgcttt tttggcagct ttatataaac gagaagaaat
     4381 aactgatatg gattatgagg aagcatttga tatgttggat gagattgaat tttaaggtat
     4441 actccttatt gaaaggggta gttatagaag tgaaaaagag atattttcta tttgttacaa
     4501 tatttataat aatagcaatt tgtgttttga cagtattttt accacaaatt actgagtatt
     4561 taaattatcg gatatttacc acaaaagtag atgatagcgt tactagttta agtaggctag
     4621 aatttataaa aatatgtgca tctgtgtttg gtcctatttt gactgtaatg gtatttgtga
     4681 atactttaaa tatgcagaaa aaaacagagc aaaagcaaaa tgagcttgaa gtaaagcaag
     4741 ctaaggctga gaacgagaga gcaaaaaaag agctgttaac acagatagat cgtgagttct
     4801 attttttatt aaatatgttt attgaaatac aaaaagatca aaatgccaaa caagctgtta
     4861 ttgctttaca gaagttggca atttttaacg aaaagggttt taatcaagac tatgcattta
     4921 cttctaaaga tttatcagat agtgaaattg ggagtacaat tgaacaatca ggaccgctga
     4981 agggcacagt tgaatatata aaatttgaaa catttgatgt aagcaagtcg atggggaatt
     5041 atcaaacaaa aacaaaagac gttgctacaa gtttacacga tcagaaagta gctatattga
     5101 atgaacaata tgaaaagatg caaatatatc ttggacggta cttcaaaatg tttcaccgaa
     5161 tagttaaaac attaaatgag tattatgacg attataatga ttttgatgtg aagaggtata
     5221 caaaatatat agggacatta agaacacaaa tttctccagc tgaattccaa gtgattttat
     5281 tcaattctct atatataaaa cgaggttttg gcttaggaat tcaacttatt ggaagtggtt
     5341 tttttggaga tgattttgat tttgaaacta atcaacattt tgaaacaagt atcaatgaac
     5401 agtggtttct atcactatca actgttgaca gtgataactc tataaagcga caaaaattat
     5461 ctgaatatat taaagaatta ggatccgtag aatataaaaa aatagctaat tttgaaagtt
     5521 tatataaact ctttaatgaa actaagctct agaaataggg cttttttatt tttctttgaa
     5581 aggt
//
LOCUS       DACDTO010000127         5468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDTO010000127 DACDTO010000000
VERSION     DACDTO010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.130"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWK70_002687"
     CDS             <1..152
                     /locus_tag="IWK70_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9615179.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IWK70_002688"
     CDS             complement(230..763)
                     /locus_tag="IWK70_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9615180.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWK70_002689"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWK70_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9615181.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWK70_002690"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWK70_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9615182.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IWK70_002691"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IWK70_002691"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9615183.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IWK70_002692"
     CDS             complement(4488..5066)
                     /locus_tag="IWK70_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9615184.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..>5468
                     /locus_tag="IWK70_002693"
     CDS             5388..>5468
                     /locus_tag="IWK70_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9615185.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLS"
BASE COUNT         1610 a         1089 c          974 g         1795 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagc
//
LOCUS       DACDTO010000128         5126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDTO010000128 DACDTO010000000
VERSION     DACDTO010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.131"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            125..796
                     /locus_tag="IWK70_002694"
     CDS             125..796
                     /locus_tag="IWK70_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9615186.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            complement(1632..2588)
                     /locus_tag="IWK70_002695"
     CDS             complement(1632..2588)
                     /locus_tag="IWK70_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615187.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(2697..2942)
                     /locus_tag="IWK70_002696"
                     /pseudo
     CDS             complement(2697..2942)
                     /locus_tag="IWK70_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3087..3509)
                     /locus_tag="IWK70_002697"
     CDS             complement(3087..3509)
                     /locus_tag="IWK70_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615188.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            complement(3648..4078)
                     /locus_tag="IWK70_002698"
                     /pseudo
     CDS             complement(3648..4078)
                     /locus_tag="IWK70_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            complement(4394..4657)
                     /locus_tag="IWK70_002699"
     CDS             complement(4394..4657)
                     /locus_tag="IWK70_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615189.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
BASE COUNT         1637 a          996 c          783 g         1710 t
ORIGIN      
        1 tgatcttatt tgagtatttt ttgttttttt aatagctagt atgttacttt gaaatttgat
       61 attatataac gagcatagaa ctaaaatatc aaattcaaaa atgaaatttt gagagggatt
      121 aaatatgaca attaaaacta gtctccaaaa tcaagcttat caatctattc gtcagcaaat
      181 tatctacgct gacttagaac ctggaaaaaa agtatcagaa aaaaaattgg aagaaacgct
      241 ttctatcggt cgtactccaa tacgtgaagc attgattcaa ctccgtcatc aaggacttgt
      301 tgatacaatt cctcaaagtg gaaattatat ctcattaatc gacttagatt tagcaaaaaa
      361 tgctcgtttt gtcagagaac atttagaacg acaaattatg ctagagtgtt gtgccaagat
      421 gaataatcaa aagaaacaag ttttagaaac tataattgca gaacaagaaa aagctgttag
      481 tcaaaagaat gctcgtagtt tttttcataa tgataattta tttcataaag cttgttttga
      541 aattgcagaa cgggaagaag tatggcaatg gttggaagat aataatacgc acttggaacg
      601 atttcgttgg ttacgagtaa aaacggaagg attaaaatgg gaaaaaatta tggaacaaca
      661 tcatcaacta tttaaagcct tgaccatgca agaccctgaa gaagctaact tcttaacatc
      721 cttgcatctt catttaatgc ttgatgaaca aaatgttgta ttgactactc acccaaaata
      781 ttttaaaaat atataactgc taaatttctt ttttttaatt tatttacatt tctgattact
      841 acagaaaact aactactttt tttatgtaaa gttctgtcaa atatagtcac tttatattac
      901 tttctcttga gtaaaaaaac atgtgaggtc gtaaacaaag attgaactat ctacattcat
      961 actctataat ctgtttattt gcagactgat aatagcaaaa gaaaacttat aatcaaataa
     1021 tatcaacatt tgatcacata atttgagatt gaatgctgat ttacctatgt tttacgtaat
     1081 ggtctttagt tatttttgct cataaaatgc ataaaacacg cgtttttatg tgtgcttgaa
     1141 atttcttggc tctataattt ctaagactga taaaaatatt ctatcggttc tttttgcaaa
     1201 taaaaaaaca tatagaagct ccattagatt gaatcaccac aacacaatca aaggagcatt
     1261 ccgtatgctt gattttcatt ctataaaact acttttagga atcaaagaaa atcacaacgg
     1321 ttcaaactaa aagcaagaat atccgaagta ccattgtttc atcgatcatg agaaaagcat
     1381 tcacctctgt ctgcagatgt tcttatatcg ctctgtcagt cctattaata aagtactgtt
     1441 ttgttttcaa aaaaactatt acgagtactt tttaagagaa tgccttgcaa atattcatca
     1501 cacaccgtta tataagtcaa atagtttctt tcagtcgtca agaaaagatt ctcctgtcct
     1561 tggcattatc agttaagtag caacttaatt ctcggacttg ttctactcta gcaaaattta
     1621 ttttcatcaa cttacgtcca aatgtttttt tgaataatta cttcatcgcg ggtctggaag
     1681 acttgcctca ttcgataagt cactagcttt tggcgtcctt caggatttct agtatacacc
     1741 aactgacatt tgggcagcga cgattgattt aattcgttta gaaaaaggtt agcgatttgc
     1801 ttatttcggc caaagcgttc tggtaccgtc agaactatct ttttcccttc gattaaatac
     1861 cgcggattct ccagcggtgc taaaagctcc ctcatagctt cagtaaaaag ctgtccttct
     1921 cgatagccag cattctcaag atagcatcta aattcctgct gatcagccgc tacgattact
     1981 tttctatcac taacttcacc aaggcgctgc aacgtcgttt gaacgcatat ggccaagctt
     2041 tttttgcggg cacattcctt ttcccatagc ttttgtcgcc tacgaatttg gaaatattcc
     2101 agcaagttac ctttaaatat agctgaccag aagcttagag gctctatatt ttttactact
     2161 atcggcaatg aagtttcttg tttttgcttt acctgatcga ctttcctaac gacgattggc
     2221 atagataggt gccagccatc agctaaagca gtactccatt gttctttcaa ccgacttcgt
     2281 tcacaagaaa aatagaacat cgactgagta taagtttcca tcgtggtggt cgtatattcc
     2341 agtggtaaag caaagcgatc gccaccactc tcaatttttg gtgaagattc aaaggtcaga
     2401 cctgaaatat tgcccaaacg ccgtagcatt cttttcaaat caggctgttc agacagagga
     2461 actcccggta atccaatcgc taaatgccaa atgttcgtta ctttattttg tgcacctagg
     2521 cgtaagcccc gccctcgtag ttgttgtgtt tgcacaaaag tggaagcttg attagccaag
     2581 atcaacagat taattgcagg acaatcccag ccttctccta gcaaactaac ggttcctacc
     2641 aaaatattca acttcttttc ggttaatagg cgagtaactg cttgcatcaa tgcttggcgg
     2701 tttttgtcat ccaccgtcaa ataaaaataa cccgacatat aaggatgttc attcataccc
     2761 gtcgctagtt ctgccagctc aacagttact agcattttcg gtaacagtaa gaactctcca
     2821 cagatggcac cgatttgctg agtgttacct aatagacgaa caacttcatc aaacaaagga
     2881 accacgccat aagctaaggt accatcactg cctgctaata cctccttgca aatacgatcc
     2941 atcaaaatga ccgctgtcag ttcttgcttc agctgaactt cttcttttaa caaaatttga
     3001 caaactgctt gctttttctc aggaatcgcc tgactaccaa cagtgtctgg atactctgga
     3061 aacaaatgca gcgactcatc aaacaatcat ccagcttttt tcaattgata aaaaataggc
     3121 aattccggtt gatcttcaaa catctgctga taaatagtga tcagctcttt tttacctaaa
     3181 gatggaactg aagctttttt accttgctgc ttagaactgc ctaatagctg ccaatgttcg
     3241 ggtatcaatt catagttatg atgattcaag taaattaatg cactagtgaa cagctcaaaa
     3301 ttttcataaa tttccttcga atgctcaagc ggatgctgaa caaattctaa agaaagcaaa
     3361 taatccaccg tctcttggct ttccattaac gcttccacca gctcattttg tttctctaaa
     3421 aaggtaaaat aatgctgagt agtttcctga ttagccggaa tcaaatatag aaaatcctga
     3481 tgaggacata gaacctcctc ctttaccaac tcgggaatgc taatttcttc gtcaatcagg
     3541 ccattttaac tcaatatatt gcttccactc cgtctgagag ctatcaatcg gaggcgtggc
     3601 tgtcaaggaa atcgtcaaca gattcggttg ttttttcttc aagtcaatca acaattcacc
     3661 ccagcttttt tttaaatgat gagcttcatc aaaaaactaa cagctccagc tgatcaccat
     3721 aagttggctc ctccttcagt cggttgtaaa tggtctgata cgtttccact gtaatatgac
     3781 taggatgttc aatcgcccca gaaagatagc aactatctgc tggatttact aagaaatatt
     3841 cctcgattgt tgttaaccac tgtttcttca atattaggga aggaaccaaa attaaggttt
     3901 tacaatccaa acgtcggatc atctcaattc ctaaaaaggt cttgccagcc cctggtggag
     3961 ccactagatg gactgaattg ttatttaaat aagtagcaaa gttagtcaat atacgcttct
     4021 gatagctacg ccactgaatt ttttcatcag atagccacat cccaggtccc cctctcacgt
     4081 attttctcta tcataatcta aatgaataca gcaaaaaaaa gatttcttca agaatatgtg
     4141 tttttatagt aatattttac ttactctaaa ctcaaatgaa aaggtaaaaa aactggtagt
     4201 acaatctttt aaattctcat tgttttgtac gttctttact atcaaaaaaa caaaaaaaac
     4261 gcgtttctac gcgtgaccaa aatttttagc tctataattt ctaagactga tagaaaatca
     4321 ttctgttttt gaagtctttt ttcataaata ctacttgatt tattgtgtat attgtataaa
     4381 agattgttca taattatttt ttataattaa aatttggtat tgatgaccag cgattccgta
     4441 tataatgcgc aatcaaatta agctttctgt ctctggtaaa tattcctgtt ctgttttctt
     4501 gtactcgtaa gatttcttca cttatcgcaa aattcataaa attccacact ttttcataaa
     4561 caaaatgtac acatttatct attacttcat aatagtctac ttgatattct tctgtaaaca
     4621 tggttgctac tgtatctgca ccatatttga taaacatttg ggctactccc ttaaattgag
     4681 aaaagcccat tagtttatct actaatactt ctaaaatttc ttgctttctt ctttaacatc
     4741 gtcccgtaag gaatcatgta gtatgctgga ggaagttcat tttctaaata attgtatcgc
     4801 ttcaattgct caaaggctat tctctacaat taattacacc aattacttta tcttcactta
     4861 tataagattt ctaaacattt ttcgtcaaat gaagagaaaa tattttttta aatgatcttg
     4921 ctcaagattt gtcaacgaat ctttgaattc gttttgaagt tctgaggaag gtatcattta
     4981 aaaactcaat ctgactttca tcatttcgtt tactcccatt ttgatcacct tctaccaaca
     5041 atttattaaa gtgattatac taggttttta tttttttaat tcacaattgt ttattcactt
     5101 catgaacaat taaacacaaa atcagt
//
LOCUS       DACDTO010000129         4846 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDTO010000129 DACDTO010000000
VERSION     DACDTO010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.132"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..258)
                     /locus_tag="IWK70_002700"
     CDS             complement(<1..258)
                     /locus_tag="IWK70_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615190.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="IWK70_002701"
     CDS             complement(274..924)
                     /locus_tag="IWK70_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615191.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="IWK70_002702"
     CDS             complement(935..2107)
                     /locus_tag="IWK70_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9615192.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="IWK70_002703"
     CDS             complement(2126..2998)
                     /locus_tag="IWK70_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9615193.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="IWK70_002704"
     CDS             complement(3020..3940)
                     /locus_tag="IWK70_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615194.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="IWK70_002705"
     CDS             complement(3940..4209)
                     /locus_tag="IWK70_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615195.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="IWK70_002706"
     CDS             complement(4206..4586)
                     /locus_tag="IWK70_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9615196.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
BASE COUNT         1273 a         1060 c          794 g         1719 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaa
//
LOCUS       DACDTO010000130         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDTO010000130 DACDTO010000000
VERSION     DACDTO010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.133"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWK70_002707"
     CDS             564..989
                     /locus_tag="IWK70_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615197.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWK70_002708"
     CDS             2141..3118
                     /locus_tag="IWK70_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9615198.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWK70_002709"
     CDS             3128..3625
                     /locus_tag="IWK70_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9615199.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWK70_002710"
     CDS             4001..4282
                     /locus_tag="IWK70_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615200.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWK70_002711"
     CDS             4284..4700
                     /locus_tag="IWK70_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615201.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDTO010000131         4713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDTO010000131 DACDTO010000000
VERSION     DACDTO010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.134"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(213..443)
                     /locus_tag="IWK70_002712"
     CDS             complement(213..443)
                     /locus_tag="IWK70_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9615202.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="IWK70_002713"
     CDS             complement(673..1491)
                     /locus_tag="IWK70_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9615203.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="IWK70_002714"
     CDS             1652..2341
                     /locus_tag="IWK70_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9615204.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="IWK70_002715"
     CDS             2355..3857
                     /locus_tag="IWK70_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9615205.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="IWK70_002716"
     CDS             3870..4346
                     /locus_tag="IWK70_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9615206.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="IWK70_002717"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="IWK70_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1590 a          673 c          830 g         1620 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACDTO010000132         4710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDTO010000132 DACDTO010000000
VERSION     DACDTO010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.135"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            106..357
                     /locus_tag="IWK70_002718"
                     /pseudo
     CDS             106..357
                     /locus_tag="IWK70_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            450..1244
                     /locus_tag="IWK70_002719"
                     /note="aph(3')-IIIa"
     CDS             450..1244
                     /locus_tag="IWK70_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9615207.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1654..1765
                     /locus_tag="IWK70_002720"
                     /pseudo
     CDS             1654..1765
                     /locus_tag="IWK70_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1786..1869
                     /locus_tag="IWK70_002721"
     CDS             1786..1869
                     /locus_tag="IWK70_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9615208.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWK70_002722"
     CDS             1994..2731
                     /gene="erm(B)"
                     /locus_tag="IWK70_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9615209.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2994..3857)
                     /locus_tag="IWK70_002723"
     CDS             complement(2994..3857)
                     /locus_tag="IWK70_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9615210.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3859..4131)
                     /locus_tag="IWK70_002724"
     CDS             complement(3859..4131)
                     /locus_tag="IWK70_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9615211.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4148..4363)
                     /locus_tag="IWK70_002725"
     CDS             complement(4148..4363)
                     /locus_tag="IWK70_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9615212.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4455..>4710)
                     /locus_tag="IWK70_002726"
     CDS             complement(4455..>4710)
                     /locus_tag="IWK70_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9615213.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1604 t
ORIGIN      
        1 ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa catgtttaga
       61 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
      121 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      181 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      241 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      301 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      361 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      421 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      481 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      541 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      601 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      661 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      721 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      781 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      841 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      901 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      961 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
     1021 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1081 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1141 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1201 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1261 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1321 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1381 cgcctacgag gaatttgtat cgataagaaa tagatttaaa aatttcgctg ttattttgta
     1441 catttaactt gacggtgaca tctctctatt gtgagttatt agtggtacag ttttcaaccg
     1501 ttttaattat aaaaaagtgg tgcattttta aattggcaca aacaggtaac ggttattgca
     1561 ggtgtatttc ttatctatgg gtttaacatg gattttatca ttaaaatcat gagtattgtc
     1621 cgagagtgat tggtcttgcg tatggttaac cctaaagtta tggaaataag acttagaagc
     1681 aaacttaaga gtgtgttgat agtgcattat cttaaaattt tgtataatag gaattgaagt
     1741 taaattagat gctaaaaatt tgtaattaag aaggagggat tcgtcatgtt ggtattccaa
     1801 atgcgttatg tagataaaac atctactgtt ttgaaacaga ctaaaaacag tgattacgca
     1861 gataaataaa tacgttagat taattcctac cagtgactaa tcttatgact ttttaaacag
     1921 ataactaaaa ttacaaacaa atcgtttaac ttctgtattt gtttatagat gtaatcactt
     1981 caggagtgat aacatgaaca aaaatataaa atattctcaa aactttttaa cgagtgaaaa
     2041 agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg tttacgaaat
     2101 tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac aggtaacgtc
     2161 tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac tgaatactcg
     2221 tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac agaggtataa
     2281 aattgttggg aatattcctt accatttaag cacacaaatt attaaaaaag tggtttttga
     2341 aagccgtgcg tctgacatct atctgattgt tgaagaagga ttctacaagc gtaccttgga
     2401 tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc aattgcttaa
     2461 gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa taaaacttac
     2521 ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact ttgtttcaaa
     2581 atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa
     2641 acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt ctatttttaa
     2701 tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt aaatttggaa
     2761 agttacacgt tactaaaggg aatgtagata aattattagg tatagggcac ctctaataac
     2821 taccaattaa ttcacctcaa aatgaaatta gaaaaaaaca gcgaaatttt taaatctatt
     2881 tcttatcgat acaaattcct cgtaggcgct cgggacccca acatgaggaa atgtttttat
     2941 aaaaaaagag tagttaccgt tttttggtaa ctactctttt ccttttttaa ggtttaaata
     3001 cctggaagtt taggtgtttt gggtattggt ggtgtaggtg gctgcaagct ttccaatttt
     3061 tgttgaattg ctttaaattc aggtgtctct tggtgtttat ttagaaccat tttttgctct
     3121 attcgttcta aagtcggttg aatttctttc cctgatactt tacgattcaa ttctttttct
     3181 aaggtttctt ttggactaat agaaggtgtt tccaaacttg aatagagttt tactccttct
     3241 ctgttatata gccttatatc gctaaataag cccgttttat gaagggtttc taaattggtc
     3301 ggtaagtttt tgacaacaat atcatgcgct tgttttggtg ttgccctggc tgtcattgga
     3361 tcatctgcat acatggtttc atatcgttca attgttccta aatatgagtt gattttaggt
     3421 actgccatga catacatttt tgtttcataa cctttggctt gaagcattgt tgcggtttga
     3481 ataggaacgt ctgttgttcg tcctgtacct tcgatcacca aattataccc ttgatcgctc
     3541 aaacggctta tgatcgcttc tgtcatgcga ttagaataag gggtaacgtg ttttactacg
     3601 tctttttcat aaagtttcac tagttcatca aaattagggt gctgttgttt aaaggtatca
     3661 ttatcaatga caataacatt cccttgtgtt tcttcaaaaa ttgctgatcg caaactggtt
     3721 ttccctgacc ctggttgccc accaagtaaa aaagcggttg gcgattcaac cgcttttttt
     3781 ccttgaatca attcttctaa attatcattt aagcgattct caaattgttt gtcagtaaaa
     3841 ttgactatat ttgccatatt aagccacttt ctctttattc aaaacttgtt ttgagtaacg
     3901 attcattgac cgccaatact catgaacggc ttgtaattct tctaaagaat aatcaaaaag
     3961 atttacacgt tttgcaagct ttaattggtt taataaattg acttccaata attgagagtc
     4021 atttttattt aattcatggt tcaaaacata gtttaatact cgattataaa cgcttgccga
     4081 taattcgtta atgatctcta tttcaaatgt tttttcatac gtaactgcca tttcattttc
     4141 actcctttta aagtttgtca ggtaaatatt tccgtatata ttcttctaag gcttgatcca
     4201 taacttcttt tacgtttccg ccattctttg ctgtttcgat ttttatgata tggtgcaagt
     4261 cagcacgaac acgaaccgtc ttatctccca ttatatcttt ttttgcactg attggtgtat
     4321 catttcgttt tgctttttgt gcgcctaaat ttcccacaat cactcacttc tttctatttc
     4381 ttcttactct tattttatca tcaacaatca caaatcacaa gtgatttgtg attaatcact
     4441 tgtgatttgt gattctattc tttttcgttt tctaattgaa taattcgctc aagcatttca
     4501 aaaaatacgt tcttatacat ggataaaact tttttgtcat agcctttatg ctctgtaatg
     4561 ccgtttttag accaggtact tactttatta cttcgcttga taatattttg gaaaaccaag
     4621 ttatcttctt tatgttgctt atacagttct tccaggtttg attttatcgt tgcgctgtcc
     4681 gtatcaacta aataaggaac aaaaccgatc
//
LOCUS       DACDTO010000133         4644 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDTO010000133 DACDTO010000000
VERSION     DACDTO010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.136"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            184..528
                     /locus_tag="IWK70_002727"
     CDS             184..528
                     /locus_tag="IWK70_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9615214.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            529..948
                     /locus_tag="IWK70_002728"
     CDS             529..948
                     /locus_tag="IWK70_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9615215.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1040..1390)
                     /locus_tag="IWK70_002729"
     CDS             complement(1040..1390)
                     /locus_tag="IWK70_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615216.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1356..2576)
                     /locus_tag="IWK70_002730"
     CDS             complement(1356..2576)
                     /locus_tag="IWK70_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9615217.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2942..3508
                     /locus_tag="IWK70_002731"
     CDS             2942..3508
                     /locus_tag="IWK70_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9615218.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3653..3740
                     /locus_tag="IWK70_002732"
     tRNA            3653..3740
                     /locus_tag="IWK70_002732"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3685..3687,aa:Ser,seq:cga)
     gene            4074..>4644
                     /locus_tag="IWK70_002733"
     CDS             4074..>4644
                     /locus_tag="IWK70_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9615219.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1688 a          780 c          894 g         1282 t
ORIGIN      
        1 aaacggcgta aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata
       61 aactaagcat tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga
      121 aaataaagtt gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga
      181 gaaatggcag aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt
      241 gcagaattga atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct
      301 catcagcgta gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg
      361 ccagctcatc tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc
      421 attacccatt tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca
      481 aaagaaatct atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa
      541 cacgaagaaa tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct
      601 caagtattta ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt
      661 ggtgtaggta atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac
      721 ggctatcaaa tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca
      781 gcaagacgat taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat
      841 ggtgcgacaa aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt
      901 ttagaacctg taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga
      961 acagctcctg aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt
     1021 ttataaatag aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat
     1081 tgataatcgg ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat
     1141 aatcttcccg atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag
     1201 ggtcccgagg attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg
     1261 ccgtaccctg tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt
     1321 cagttatttc atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt
     1381 ctttttccaa gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca
     1441 aggatacctg caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc
     1501 aaagtgctga cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa
     1561 ctgccgtaaa aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg
     1621 ccagtactgg tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt
     1681 atcggaaaac tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata
     1741 gcaccgatga caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc
     1801 agaccaacta agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca
     1861 gttgtcaatt ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta
     1921 taatagaaac caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga
     1981 aagtgatatt cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa
     2041 acagcagtaa aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat
     2101 ccacgatgga aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg
     2161 ccagctaaat tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata
     2221 taactaacga ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa
     2281 ggtttattga caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa
     2341 aggtttttgc caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg
     2401 tttaaaatca ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca
     2461 tttatgaatg cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc
     2521 gaggtaagaa tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca
     2581 ataaaaagaa aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga
     2641 ttcgtttttg aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt
     2701 ttcattttct ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa
     2761 actaaaaatg taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa
     2821 gttaaagaag actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa
     2881 atgctaaaaa tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt
     2941 aatgagaaga aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc
     3001 aaaggaaggg tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac
     3061 acagccgatt tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc
     3121 tatctttgaa caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat
     3181 tgatctcggt ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt
     3241 gtacattgat gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg
     3301 tgaaatagtt aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct
     3361 gcatactggt acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat
     3421 gcatccaaca gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc
     3481 attagataat cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc
     3541 cgttggctgc aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc
     3601 tattagaaat aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt
     3661 ggcagagtgg taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg
     3721 ttcaaatcct gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat
     3781 caagtaataa gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt
     3841 attaccgcag atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac
     3901 ttttgtcaca gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga
     3961 gtactatttt ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa
     4021 atactagatg taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta
     4081 ccgcaatcac ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt
     4141 cttataatga agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa
     4201 aaaatttaga taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac
     4261 tctattatga ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca
     4321 atgctgatta taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc
     4381 catggatact agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca
     4441 atctagtcaa tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag
     4501 ctgataaaga aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata
     4561 atcctgtcgg tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca
     4621 attatcgctc gctatcaaat ggaa
//
LOCUS       DACDTO010000134         4610 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDTO010000134 DACDTO010000000
VERSION     DACDTO010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.137"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(85..339)
                     /locus_tag="IWK70_002734"
     CDS             complement(85..339)
                     /locus_tag="IWK70_002734"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9615220.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(450..704)
                     /locus_tag="IWK70_002735"
     CDS             complement(450..704)
                     /locus_tag="IWK70_002735"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9615221.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(897..1172)
                     /locus_tag="IWK70_002736"
     CDS             complement(897..1172)
                     /locus_tag="IWK70_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9615222.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1144..2097)
                     /locus_tag="IWK70_002737"
     CDS             complement(1144..2097)
                     /locus_tag="IWK70_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9615223.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2709..4202
                     /locus_tag="IWK70_002738"
     CDS             2709..4202
                     /locus_tag="IWK70_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9615224.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4337..>4610
                     /locus_tag="IWK70_002739"
     CDS             4337..>4610
                     /locus_tag="IWK70_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9615225.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1542 t
ORIGIN      
        1 tgcttgaatt cttgtgaaaa agttcttcgt gttctcttag acataaaaat tcctcctggt
       61 atgttttctt ctagtctaca caccttaatt tttctgtcta gttaattgta gcctatccat
      121 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
      181 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      241 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      301 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      361 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      421 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      481 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      541 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      601 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      661 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      721 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      781 ccaaatgttg tataccccca aacaatacac atattttgaa ggattaggat taataaagct
      841 aaaaatatag taagtatact aaatatattt actatatcta gtatacttac tatattttaa
      901 tcttttgatc gtctattata tataatccgt tcctcgtcgt ttaaagtatc aacataggcg
      961 tccattgcaa gtttgactat ttcatacata ggaatatctt ttgcataact aagatcccga
     1021 atggttttta gtacgggagg atctacttga actggttttc tttctttcgc agtgtacttt
     1081 cgttccccta actcgacatt aatatcatct ttaattgaaa tatcttcagc accaattttc
     1141 ttcttatctt tgttcgttgg taataatgcc atttagttta atctcctttc caagaggtag
     1201 gatatgattg gccattgagt cagcatattt aggtgtataa tcaaatcctt caatatcacc
     1261 atgttgttca tagtattcaa ttctttcttc taattctgta aagatgtctg caaaaatagc
     1321 ccaccatctt cgatctacat aatcatttaa ttggatacca ttctcaccat acatttctaa
     1381 ccgatccgag cctttaacaa tgcttttaaa aatgtttgat tcaccatact tatcaattgt
     1441 tttttgatag tttgcgtgtt gagaggaacg tcgcactgag aaaagaacag gcaaaattcc
     1501 aatgatttga agatgagact cttcaaaatt cgtgacaatc ggaactaaaa ctttattaat
     1561 aaaatcttct gttccttcca ttgcgtaccg ttttaattct tgcattgcaa taatataatc
     1621 tgcagcagtt aagaatgcac gaaccacatt atctgttgat ggtgggcagt cgataaagat
     1681 ataatcgaaa ttttttctca attcttctaa aggtttaatg aaggccaatg atctcttacg
     1741 acgatcagga tatttttcgg acaagtattc attaagttct tcagatccag gagagccagc
     1801 cataatatat aaatgtggac ttacttgagt aatacctgaa acccagtccc cttttttgat
     1861 tgctgcagac atggatcgat caaagctagt aatttgatac gttgatccaa tgttggaagt
     1921 agtatttctt tgttggtcat aatcaattag caacacgtct ttttcaaaaa gttgactaga
     1981 aacaatcgct aataaatcat tgtctgtagt cttaccaatg ccgccttttc tacctgtaag
     2041 cattacaact tttcctagtt tatctgatgt tttaaatgca gtccgtaagt tttccatagt
     2101 tccctccaat ttcctatact aaatatattt agtatacttc atatacttaa tatattcgat
     2161 atatctaata tactccaaat tgataagaat tcagacttta taaggaatat agtggatata
     2221 ctaaatatat ttgctatatt cagtatatca gatatattca aaatgtcaaa atatatttgt
     2281 gcgttttgcg ttgacaattt agagtgaaaa gaggtaaact taattctata gaaaccacag
     2341 ataattcact atttacaaag tagcaaaagc agtggtactc tttaaacaga aaaattacat
     2401 aaaaaaaaga gacggaaata attgatttta gcagaatcaa ttatttctga atcacatggt
     2461 taggacatgt gttcacgctc gtctcaagaa tatgtaagta gctaccaact acttatatta
     2521 aaagttgatc ggataccttt tccgattgcc tttattctac caaaaaaagg cgagcaactc
     2581 aacagaagaa cgtttattta gtgtacttaa attaaatatt cttaaacact gaagagagaa
     2641 cacatactat ggctgagatt aaagctaaac tgtgttctct ttttgtttga gaaaggacgt
     2701 tacaatcgat gttgagcgaa atttattcag ctattcttaa aaacggctta cgtacataca
     2761 aatatcgaaa cagcaccatg aaacctgcag gtcatattga ttccagtagg agtggtgcta
     2821 tctttgcgta ccgtagcaag caattaatga atgttgggcg tggtatggtc attacttcgg
     2881 aagaagctat tttagagaac gaaaagaaac taacgcattg gacacctaat acctatcgct
     2941 ttggtactta tgcggatgat tatcgaacag tggttaaagg tcattctgag aaaaacttaa
     3001 gtcaaatcaa cacatttgta gttgatatcg attcaaaaga gaaccatcaa ggcgaaatta
     3061 ttttagcctg tttagatcaa gtgggctaca tgccaacgct aattttagaa tcagatcatg
     3121 gttatcaagt ttattttgta ctaaagacac caggctatgt cacaaagaaa agcaatttca
     3181 aggtgatcga tgtagcaaaa aaaatttcaa aaacaattcg tctacagttg gctgaaaaat
     3241 tgtccggagt ggatctagga tgtaaccact tcggaattgc tcgattccca caagaaaata
     3301 atattgtttt taaagaaatg aactatcagt attcttttgc tgactggatt caatggtcga
     3361 tgaagcttga atcagatcaa aaaacaaaag aagatcgaga acgaaaacta attgtctttc
     3421 ctgagaaaaa agaaattcgt caagtagatg aaccgtggtt cgatatgtta cttcataaag
     3481 caaacatcgt cggcggagaa ggacgtcttg gtcgaaataa tgtgattttc acgctttctt
     3541 tagcttatta ctcttcaggt tatgcattgg atacttgcga atacaatatg ttcacattca
     3601 acgaacggtt acaggaaccg ctatctgaag cagaattagt aaagattgta cgtagtgctt
     3661 attctggtaa ttatcaagca gcaagcagag aattcattat tgaattgtgc cagcaatggg
     3721 tttcaccaac cttaaaaaat aatgatctgt tcattcaacg acgtggctgg tggaaattta
     3781 aaaagccacg agatcaaaga aaatatagtc ataaaaatga atggaaagaa gatctattgg
     3841 cttatctaag cgaaaaaagt cttgctagtg agccttattt gaacctgtca aaaaaagaat
     3901 tggcttcaca actaacgatg cccaagcgaa cacttgataa gatcttaaat gagttacgag
     3961 aggagaataa agtcttctac aaggtaacac gaggaagaaa tggcggaatg atcattgctt
     4021 cagtcaaaac gttgttcgcg agggttattc aactaagtaa agaaaagaga ctagattatt
     4081 caacaaggat cacagaggta tttggactca acgagagctt agtagaagag acatttagac
     4141 aattatcaac ccccttgaac cctcttaaag aagtgaatct ttttgaagta gatacaggtt
     4201 gaaaaataaa aggtgcaaat tgccttatac aatatatcta tgatacttct gtaggtgttg
     4261 ctccctttgc ctagctacgt gtaggtggta tacttataac tgcgtatgca gtcttatgta
     4321 tcatggagga agttgtatgt cgttaaaaaa ttatgtctat tcacatcctg ttaatgtgtt
     4381 tatcattggt cgtttgggat tatctgtgga agtgttctgt gagctttatg gttttaagca
     4441 ggggactttg tcttcttggg ttaccaggga aaaaacagtt gcttctttgc cgattgagtt
     4501 tcttcatggg ttgtccttag cttctgggga gactatggat caggtatatg attgtttgtg
     4561 tgtgttagag caggagtata ttgagtataa aatagctaac gagttaagaa
//
LOCUS       DACDTO010000135         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDTO010000135 DACDTO010000000
VERSION     DACDTO010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.138"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWK70_002740"
     CDS             690..1658
                     /locus_tag="IWK70_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9615226.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWK70_002741"
     CDS             1669..2484
                     /locus_tag="IWK70_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9615227.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWK70_002742"
     CDS             2552..3262
                     /locus_tag="IWK70_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9615228.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWK70_002743"
     CDS             3298..>4425
                     /locus_tag="IWK70_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9615229.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACDTO010000136         4347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDTO010000136 DACDTO010000000
VERSION     DACDTO010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.139"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IWK70_002744"
     CDS             complement(387..1853)
                     /locus_tag="IWK70_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9615230.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWK70_002745"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWK70_002745"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9615231.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1414 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaatt
//
LOCUS       DACDTO010000137         4310 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDTO010000137 DACDTO010000000
VERSION     DACDTO010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.140"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(65..1285)
                     /locus_tag="IWK70_002746"
     CDS             complement(65..1285)
                     /locus_tag="IWK70_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9615232.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1305..1910)
                     /locus_tag="IWK70_002747"
     CDS             complement(1305..1910)
                     /locus_tag="IWK70_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9615233.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1959..2891)
                     /locus_tag="IWK70_002748"
     CDS             complement(1959..2891)
                     /locus_tag="IWK70_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9615234.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3312..4172)
                     /locus_tag="IWK70_002749"
     CDS             complement(3312..4172)
                     /locus_tag="IWK70_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9615235.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1230 a          866 c          707 g         1507 t
ORIGIN      
        1 agcttaaatc atagataccg taagggactt tattctttat ttaaaacttt gcaacagaac
       61 ctttttattt tacagaagca ctcgaaactg catctactgc agtttcatcg tgtgtgctgg
      121 cgctggttac tgcatctgtt tttccaccat gaacagatgc acttgaaacc gcatcttcat
      181 ttccaacaag ttgttgaccc gtttgttgta aataccactc aatgattggt ttgaaatcaa
      241 gaatgtattc attgattcca gcatactctc cattttcatc atgcattgct tggtaattat
      301 gcacaatata tttttctggc acttgttttg gtacatgaac acgtaccgca tctgttgctc
      361 cgcttcgcag ttgttgaatt acccactcta catttttata cgatttttct gggtgacagt
      421 tggctaatgg attccctacc tgaccgggca cacgagcagc taacatctct ttcgcttctt
      481 ggcgcaaatt ataatataaa aattggttat tattatctgc aaaagttaat tccatcggca
      541 tcgaatctaa aaagtaattg atttgatcca ctgtcaataa cccacgattt aatttgacgt
      601 aagtatttcc agaaacggca ccgactgctt cagaagcttt ttccaaccaa tgatcatctg
      661 acatatcaac atcgggtatt gtcgtagcaa ttgttcctgt cgcatgcaaa tcttctggtg
      721 gaagcttttt aggccctgct aaggtagaca cgactttaat gaattttaca aaacttgcta
      781 atgtactgcc taaaaagtca acagttcgtg gatcaatcaa gcgatcttct tggtcaaatg
      841 cttcttttgc ttttcccaat aaaaattcat ttccgggcat cactaatgca ttcacgcctg
      901 gtgcgtccaa aatttggcgc aagtgtaatt gcgcgcgtga agttccttga tcgtagtaag
      961 aagcacccac aaccattaca ggtttatttt caagtggatg gattttaaat gatagccatt
     1021 caagtgcact tttcaatcct gctggaaccg tatgattgtg ttctggcgta gcaataatta
     1081 ccccatcagc cattaagatt ttgcgattta gctgttgaat agcaggacta tttgtttgat
     1141 catcatcttg attgaacaga ggtaaatctt tgatttcaat gatttctaaa ttaaatagtt
     1201 catgatattg tttttggata aatgttaata atttacggtt atatgaaagt tcagctgctg
     1261 atccgacgat acctattaat ttcatctttg tttgctcccc tcgattaaat cgtttcccat
     1321 gaaaagcttt cggcttcacg tttatttgct tggtgcgcat gttgcaattg actagtgatt
     1381 gtgacaaaga gtaagaaatc tttgaatagt tcacgtaatt ttgcgacttg ttcttcatca
     1441 atgagtcgtc cttgctcatc aaaggcttgg agtgaatgac ccaataaaaa ttctgagctt
     1501 ggcataattc gtgcctttaa ctctggtgaa tccaatattt ggcgcaaatg tgcttgtgcg
     1561 cgtgaagaac ctaacgtgcc ataagacgct ccggtaatca tcactggttt atccacaaac
     1621 ggaaaaatac catatgaaag ccaatttaat gcgttaatca atgaagcagg aaccgcatgg
     1681 tcatactctg gtgtgctgat gattacgcca tctgcttgtt caatttttcc agcaatttct
     1741 ttaacaacat ttggtagttc tgtgttttca ggtttgttaa atagtggcag ctccttgatt
     1801 tcgacaattt cgatttctgc ctcatttaca aaataatttt tcataaactg taataattca
     1861 cggtttgttg accgcgttga atttgttcct actaatccaa taaatttcat cttcattctc
     1921 tttcctttct aacatcgatt tctgactgtt cgcacgtact aaattttttc aaatcctttt
     1981 gtatacaaga attgattctc ttttgttaca actgctccct cgatacccgg ctgttcttca
     2041 agcgctttca acacttctaa agaagatgct ccaaataaac gtgtagtcca gatttctcca
     2101 tctacagaag aatcagacaa aatcgtcagg cttgctacgt catttttaac tggaaagcca
     2161 gttttcggat caatgatgtg atgatacact gacgaaccat tttgtagata acgttcgtag
     2221 attccagagg taactaaaga ttggttgttt agtttaacca ccgcaagcga ctcttcacgt
     2281 gaacctttag gatttcgcaa accaatacgc caagcaccat ccgaacgtct tggtgattca
     2341 ccaaaaacaa cgatatttcc tcctaagtta actaaagcaa acgctgcatt tcgctctcgg
     2401 caatgtgtaa ttaatttatc tgcaatgtaa ccttttgcta acgcccccaa gtcaatcatc
     2461 attcctggtt tggttaaaaa aacggttttc attttttcat ctaattgaat ttggtgtgga
     2521 tccgttagca gaagtaactc agttatctct gcttttgttg gaaccaatgc atcttcaaat
     2581 ccaatgtgcc atttttgtac aaccggacca atagcaatat tcagataact atttcttgca
     2641 caactatgtg cttttcctaa tttgatgagc tcaaaaagtt caggatgtac aatcacagct
     2701 tgtcgtccag cagcttgatt gatggccatt aattcagaag ttgcatcatg tgcgctgaag
     2761 cgatgttcat aaacgtgcaa ccattcgcct aactctgcta gtaatcgact aggctcttca
     2821 tgcccaactt gtaatgtaat tgttgtcccc atcatttgcc agattttcgt ttcttcgatc
     2881 acctgattca ttttgtacct tctttacttg aactacggat tacatgagta attagttttt
     2941 atttttgtta ctgttttcac aaccagatga tacgcctgct agatttattt gtcaaccgat
     3001 tttatatatt tttttatata ataaggtaat tttaatataa aattttatat tgtaaaaaaa
     3061 caaataaggg aaacaaacaa cacatttttg tgtgttgctt atttccctct aactatcgtt
     3121 ttctatttga tggttaacct acttaaaata aatactgcaa cgacaaacgg agatcaagca
     3181 tgctttgtaa cgtattttac gataattacg actttatttt attactctat cttgctgcca
     3241 tacccgtcta tccctaatca tctttggtga attttttcaa agttagcata acaaaaataa
     3301 gtattttttc cttagttaat caatagtatc atttttttca tatgatttaa ttcatcaatt
     3361 aattttttgt ttttattaat tttagcaaaa gctagtgcat cttgaatcaa ttcgagcact
     3421 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
     3481 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
     3541 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
     3601 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
     3661 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
     3721 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
     3781 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
     3841 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
     3901 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
     3961 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
     4021 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
     4081 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
     4141 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
     4201 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
     4261 aaagttttaa ataaagaata aagtccctta cggtatctat gatttaagct
//
LOCUS       DACDTO010000138         4308 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDTO010000138 DACDTO010000000
VERSION     DACDTO010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.141"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1383
                     /locus_tag="IWK70_002750"
     CDS             <1..1383
                     /locus_tag="IWK70_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615236.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
     gene            1543..1989
                     /locus_tag="IWK70_002751"
     CDS             1543..1989
                     /locus_tag="IWK70_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615237.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1991..2128
                     /locus_tag="IWK70_002752"
     CDS             1991..2128
                     /locus_tag="IWK70_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9615238.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            2165..2458
                     /locus_tag="IWK70_002753"
     CDS             2165..2458
                     /locus_tag="IWK70_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615239.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERRK"
     gene            2455..2679
                     /locus_tag="IWK70_002754"
     CDS             2455..2679
                     /locus_tag="IWK70_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9615240.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2676..3695
                     /locus_tag="IWK70_002755"
     CDS             2676..3695
                     /locus_tag="IWK70_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9615241.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            3884..3957
                     /locus_tag="IWK70_002756"
     tRNA            3884..3957
                     /locus_tag="IWK70_002756"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3918..3920,aa:Ile,seq:cat)
BASE COUNT         1494 a          747 c          879 g         1188 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca acagccacac cgtaccagcc taacttacta
      481 gatgaccctt actgggtagg taaaacatcg ttgggtgaga atattgcaaa taaaggtacg
      541 acgttcccaa ttaaatctag cgcgtacgaa atatataaag gtaacacgga agaagagctt
      601 ataataggtc aaacgtatac tatcacgctt aaaggaacaa aacccgcaag tcaaaccttt
      661 gtagcatata attattggaa cgttaatttt ggagaactaa aaccagttga gggattgaca
      721 gacgtatggt ctctaacatt cacaccaacg aaacttgaac cgggtttgcc taaagacctt
      781 cgcatttttc agttgcctaa agaaacggcc ggcgcatgcc aaattgactg gctcaaaatt
      841 gaaaaaggcg acacccgaac cccgaatatt agtcagttta aatactttgg tgaaggattg
      901 aaagatagca acaatccgaa cgactacagc tgggacatca cgcctgaata tactgaaaaa
      961 ggcttgaatg atacggttag tttgactgaa ccgcaatcca tcgaaggatt gaaaaatttc
     1021 gaagatggta ttcaatcaaa aggaaaatct gtattgacat cagacgacaa caaatatgaa
     1081 gtcgtaacct taacagttac aaacgggaat accggatcag caaagcttta tcgtgaagga
     1141 aaaaccgtca ctatttattt ttttgcgttg aatgggaaaa gcagtggtgg aaatgattca
     1201 acgatactaa caattccaga aggctatcgg ccaccaatta gttttgagca actggttggc
     1261 tcgatagacc gttctacttt gaacagtgct cagttatcta ttggtgcaga tggagccatt
     1321 aaatggcgaa gaaactcaag ttatggatca gcttattcat ttgttatcac ttattcaatt
     1381 taagggagga aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca
     1441 aatcattgtg aatgacgaag aacatttacc ggaagaatgc acagaagtcg aaccaccgat
     1501 tccaaattgg aaaccgaaat tcaattactg ggagggaaat aaatgaaaaa catttggaaa
     1561 tatggccgta ctggcggaga gtatgcagga aaagtattgg acgacatgct tgtatccgtt
     1621 ccttacacag atcagccacc gctcgaaggg gttcgttcag atggcgaacc gctaacgatt
     1681 gctgatcaga tgtttgatcc taaattgaac caatggatta ttttagcgaa cgcgttagat
     1741 cacaacgatt taaacaatct caaagcgatg tacgaggctc ttgaacatga aaacggcaac
     1801 ctaaaacagc taaatgccaa actcatgcta agcgatgtag cgattaaaca ggaaaatact
     1861 gcattgaaag aaaaagctga cagtttagca caaatcaatt caaaaatgat gcttgcttcg
     1921 ttacaaaata gcaaagacat ttcagaaatt aaagagcaac taaatccagc ttcaaaagga
     1981 ggtgagtagt atgtttagtt ttagcgatgt gaaaatgatg tatgattggg gctgttttac
     2041 tgacgatcaa gttcgactat tcgttccact atgcattaca gacgaagaag cagataaaat
     2101 cattaataaa gataagagcg catcttaatt gatgcgtttt tatttaaggt aaaggagttg
     2161 tcacatgatt aatttagggg aatggggaat gatagcagga tcaataaccg ctatcgtttc
     2221 tttgatttta ttagtaatta gaccgattgc tgcatctttc tcgaaaatta ctgagactct
     2281 ttcaaaagta agccacaatt tagatttgct gactaaagat ttagaatcga gcaaatcaga
     2341 tcgattgatg attcatgaag aactaaagaa acacgatgaa agattagata cacatacaga
     2401 aaaattggtg gaacacacgc aacaaatcaa aactttgttt agggagagaa gaaaatgaat
     2461 aataaaacgt tcgaagtact aaaatggttc gcactggtaa ttattcccgc actagctact
     2521 ttcgtggggt tagttggtaa agcgctcaat tggcagtaca cagatatctg tgttgtcatc
     2581 attactggtt ttggcgcgtt tttagggagt gtgttgggtg tatcaaatcg aacctacaaa
     2641 atgttctcgg ctgaaagcga agaaggagga aacaaatgaa aaagaaaatt actattactg
     2701 cgatgagcct gttaacggct ctttttttat tgccaattaa cggatttgcc tatactatca
     2761 acaatgaatt taatttgggc gcaaatgaag gtagctcaca agtagcaaat aatcagtata
     2821 ttttactgca tgaaacggct aatgaaacag caacaggacg caatgaagcg cagtatatgc
     2881 aacgttcatg gactagcgct tatactgctt atattgtggg agacggcgga attgtttacc
     2941 aagtcggaca acctggttat gtacagtacg gtgctggttc atatgctaat gccaatagtc
     3001 ctgtgcagat tgagttacaa cacacacatg ataaagcaac ttttgaaaaa aactataaag
     3061 catacgttga attggctaga gattcagcaa tgaaatatgg tattccatta acattagaca
     3121 cgccttataa ccaaccagga atcaaatcac atttatgggt aactcaaaac atttggggcg
     3181 atcacactga tccgtatgga tacttatcag aaatgggtgt aagtaaggaa aaactggctt
     3241 acgatttagc tcatggattt acagacgaaa acccaacgac ttctgagaac aagcctgtca
     3301 ttgatccaac ccgagcaggt gcagcaaatc caactttatc agatggaacg aaccattctc
     3361 acattgatca gtttggagaa atcgaaaatg cgaacttaca cgtcgctgga tggcacatcg
     3421 ctaactataa gtacgagtat attttcatta tggactacaa tactgggaaa gaattagctc
     3481 gagtaagagc tgatggaatt tatagaccgg atgtaaatca agcttataat acttctggaa
     3541 atgttggcta tcatgtatct tttaacatgc gtaacttccc taataagaaa gtctatgtca
     3601 tgatgcgggc aacgaatgat ccagagggaa acactaaagg cggtgcgcaa gatttccatg
     3661 acaaacgttg gtatttaaat attcctaaac gataaaaata gctcctcgtt gaggagcatt
     3721 acataactat attgacaact ataaaaatca ttcgataaaa tagtgatgtt atcgcatatc
     3781 ttcactatca cccataatag tcacactcca agctatgcga taacaggttt gttgccacac
     3841 attctactgg ttgattgttt atggctttat gtggcaacaa ccagtaccct tagctcagtt
     3901 ggtcagagca gacggctcat aaccgtccgg tcgtaggttc gagtcctaca gggtacatta
     3961 acgtagccat ttgaatcgtt ctgtgttaga attttttgaa gagtattata caagctaaag
     4021 cttttcttca ttgccactca aatgagtggc ttttttatgt atccttttat ggattaatga
     4081 aaggatgttt cacatagtta tatttctgta tatttgaaaa gttttacttt gatttttaaa
     4141 tagaaagaca tttgggttat attgtgagat aataataaag aagagtttaa agcgttcccc
     4201 aaaaaccact gccccataag tgtgttacgc tttaaactct tttatatttg aagccattaa
     4261 aaagcatacc atatttttga aaaaaagtga gaaaaaaggc ttataatt
//
LOCUS       DACDTO010000139         4194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDTO010000139 DACDTO010000000
VERSION     DACDTO010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.142"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            1..492
                     /locus_tag="IWK70_002757"
                     /pseudo
     CDS             1..492
                     /locus_tag="IWK70_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="IWK70_002758"
                     /pseudo
     CDS             712..1008
                     /locus_tag="IWK70_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1171..1617
                     /locus_tag="IWK70_002759"
     CDS             1171..1617
                     /locus_tag="IWK70_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615242.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="IWK70_002760"
     CDS             1619..1756
                     /locus_tag="IWK70_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9615243.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..2087
                     /locus_tag="IWK70_002761"
     CDS             1794..2087
                     /locus_tag="IWK70_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615244.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2084..2308
                     /locus_tag="IWK70_002762"
     CDS             2084..2308
                     /locus_tag="IWK70_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP9615245.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            2305..3330
                     /locus_tag="IWK70_002763"
     CDS             2305..3330
                     /locus_tag="IWK70_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9615246.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            3520..3593
                     /locus_tag="IWK70_002764"
     tRNA            3520..3593
                     /locus_tag="IWK70_002764"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3554..3556,aa:Ile,seq:cat)
BASE COUNT         1450 a          719 c          842 g         1183 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa actttattta gggagagaag aaaatgaata ataaaacgtt
     2101 cgaagtacta aaatggttcg cactggtaat tattcccgca ctagctactt tcgtggggtt
     2161 agttggtaaa gcgctcaatt ggcagtacac agatatctgt gttgtcatca ttactggttt
     2221 tggcgcgttt ttagggagtg tgttgggtgt atcaaatcga acctacaaaa tgttctcggc
     2281 tgaaagcgaa gaaggaggaa acaaatgaaa aagaaaatta ctattactgc gatgagcctg
     2341 ttaacggctc tttttttatt acccattaat acgtttgctt atactattaa tgacgagtat
     2401 aatttagcgc cgaatcaagg agactccaga ttagcaattc ctaacaaaat tattttgcac
     2461 gaaactggaa tagatgcacc agcaagaaac gtagccgcca acatgaaaaa taattataac
     2521 ggaagcaatt cttatactac agatgttatt ggtgacggtg ggattgttta ccgtgtgggt
     2581 gagcaaggat atgtttcgtg gggagctggt aacgctaatc cttatgcgcc tgtacagatt
     2641 gaattacagc gcacatatga taaagcattg tttgaaaaaa actatcgagc ttatattgaa
     2701 tatacaagag atagtgcaaa aaaatatgga attccattga ctcttgatca aggaacttct
     2761 ttatttacaa aaggaatcat ttctcatttg tgggtgacaa attatgtttg ggggaaccac
     2821 acagatccat atggttactt atcgcaaatg ggagttagca aagaaaagct tgcttatgat
     2881 ttagctcatg gatttaccga tgaaaatcca acaacttctg aaaacaagcc tgtcattgat
     2941 ccaacacgag ctggtgcagc taatcctacg ctgacagatg gaacaaatca cgcccacatt
     3001 gatcagtttg gggaaatcga aaacgcaaac ttgcatgtgg ctggatggca cattgctaac
     3061 tataaatacg agtatatttt cattatggac tacaatactg ggaaagaatt agctcgagta
     3121 agagctgatg gaatttatag accagatgta aaccaagctt ataatacttc tggaaatgtt
     3181 ggttatcatg tatctttcaa tatgcgtaat tttcctagta agaaagtcta tgtaatgatg
     3241 cgggcaacga atgatccaga gggaaacact aaaggcggtg cgcaagattt ccatgacaaa
     3301 cgttggtatt taaatattcc taaacgataa aaatagctcc tcgttgagga gcagtacata
     3361 actatattga caactataaa aattattcga taaaatagtg atgttatcgc atatcttcac
     3421 tatcacccat aaatagtcac actccaagct atgcgataac aggtttgttg ccacacattc
     3481 tactggttga ttgtttatgg ctttatgtgg caacaaccag tacccttagc tcagttggtc
     3541 agagcagacg gctcataacc gtccggtcgt aggttcgagt cctacagggt acattaacgt
     3601 agccatttga atcgttctgt gttagaattt ttttgaagag tattatacaa gctaaagctt
     3661 ttcttcattg ccactcaaat gagtggcttt tttatgtatc cttttatgga ttaatgaaag
     3721 gatgtttcac atagttatac ttctgtatat ttgaaaagtt ttactttgat ttttaaatag
     3781 aaagacattt gggttaaatt gtgagataat aataaagaag agtttaaagc gcaccccaaa
     3841 ccacttcccc ataagtgtgt tacgctttaa actcttttat atttgaagcc attaaaaagc
     3901 ataccatatt tttgaaaaaa agtgagaaaa aaggcttaca attggagtgg tagttaatta
     3961 gtgacttatt tttgatttta tagcactgat actataaaat atagatatca tcatattaca
     4021 caatcttaat actaacttaa aaaatatctc ctttcacaag tatggtgata aaattcgttc
     4081 cgggctacct ttttaggtag cctactttaa tctttatacc tttctggatc aacgaaagta
     4141 tactttatat agtcataacg ccgatgatcg ctacgtgcgt ccggcacgtc agtc
//
LOCUS       DACDTO010000140         4193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDTO010000140 DACDTO010000000
VERSION     DACDTO010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.143"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(599..1141)
                     /locus_tag="IWK70_002765"
     CDS             complement(599..1141)
                     /locus_tag="IWK70_002765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615247.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(1918..2108)
                     /locus_tag="IWK70_002766"
                     /pseudo
     CDS             complement(1918..2108)
                     /locus_tag="IWK70_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2228..2590)
                     /locus_tag="IWK70_002767"
     CDS             complement(2228..2590)
                     /locus_tag="IWK70_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615248.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2594..2902)
                     /locus_tag="IWK70_002768"
     CDS             complement(2594..2902)
                     /locus_tag="IWK70_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615249.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(2911..3330)
                     /locus_tag="IWK70_002769"
     CDS             complement(2911..3330)
                     /locus_tag="IWK70_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615250.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3323..3556)
                     /locus_tag="IWK70_002770"
     CDS             complement(3323..3556)
                     /locus_tag="IWK70_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615251.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3553..>4193)
                     /locus_tag="IWK70_002771"
     CDS             complement(3553..>4193)
                     /locus_tag="IWK70_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9615252.1"
                     /translation="PNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQ
                     YRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWR
                     LELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKN
                     TKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1177 a          935 c          605 g         1476 t
ORIGIN      
        1 aatattttct ttcagttttc cgactatggt aattcggtca attgaccact gcatttttaa
       61 gggaagggtc aaactttgat actttaaccg cctgttagag ggggctgtta atttttccca
      121 ctcatttatg atcgtgcctt ttttactcaa attttgttcc tcctcaaaga aaaagagact
      181 tctggaaaga attttcaaaa gttcacgtaa ctcttaaaaa ttcattacca aaaaatctct
      241 agtcttgaag aaatctaaaa atattcgtat aatgattaag aagttacgga gctagtgcga
      301 ttagtttccg taaaggtcga gaacgtgtcc agcgttgctc gactttattt ttttgctctt
      361 ttttcagttt atttatttga taactcaata atagcacttc tctttagcta tgcaaatatc
      421 caaaaaataa aaccgcaccc tttgaaaagg gcgcccccaa acttcgctaa cgctaggttg
      481 gccaaaaaat ggacttgcca ttttttagca aatgtcagct tcagggatta gtggtttagc
      541 cgtcaactcc ttatcctcgg cacaaaccgc ctccggtacc gttgactgca catcgtaact
      601 accactcgat ctcatcttca gaacgctcta atttacgctg tgagacgttt tgacgataaa
      661 ataaggtttt ctcctgctct aacacttcta attcctcaga aagccccata tccttttctg
      721 caagaccaaa acggatagac aaacgagtga tcgatttttg aaatgcgttt tttaatcgct
      781 ctaaacgctg gcgtaaagag cgattttctt ctttcagctt tttattttcc acttgtaaat
      841 ctgctaattg ttttctacca gacaaatgac gctcttcttc caatgtagct acatactcat
      901 ttaattctgt attttgctca agcatctgta aatattgctc actcatgtta tcagcatttc
      961 tcttggcatt tgttgctctc gtagctcgtt gatacaaatc aacaaaatca ttcccagata
     1021 aatgatacga accatcagga agcctcttga aactttctaa aggaacaata tctttttgga
     1081 aaatgaccgc catctctttt tttacattca gcatattttt cttctcttct tcaatcttca
     1141 ctctctcaga agccaccaga gccttttctc gctcattttc ttctctttgc ctagaaaaat
     1201 atacctcaac tcatcagaaa cgctctcacg ctcttttacg gccgtttgac gcaattctga
     1261 aatctcgctc atgatttctt tatattcgtg aacatctttg attttgttag taccgactaa
     1321 ctcacgctcc caaccaaaat ctgccatcaa acgttcaatt gactcaactt cctgattcct
     1381 aaaattattc caaatcactc gaccattctc tttatctgct cgtaaaccct gctgttgcaa
     1441 ggctttgtta aaacttggtt gcttttgaac accccgttta tacccttcag caacaggaac
     1501 aatattcaaa tgcaggtgcg gacttacttc gtcattatga ataacagcat tgtagacgga
     1561 taaattagga ttccgttctt taaattcttt gaaatattca actaaaattt tgttggcaat
     1621 cactcgattt tgttctgctt gttgttccga tatgccagta gaagaaccat catcacgagt
     1681 agtacgaaaa tcatcaagag taccaatttg cacgataaat tctcgctgtg gttccaattt
     1741 tttatccttg cgaacctttg ctaaatagtc ctcgattttt ctatcggctc ttttctgctt
     1801 tgcattatat ttttctacgg cttcaccgaa taattcttca tatttttcac gaatatttgt
     1861 ttgttctaaa tagatatttt catccgattt tgtgaaatca atatgcttat gccagtcatt
     1921 gattttttgg ctatcagtta actcacgatt attatgagaa atgcttgtat tctttgttcc
     1981 ctttttgaac gaaatactga accccaagtt aaaaccctcc ttcggctgtc gctaataaaa
     2041 agagtgtaaa cctttctgtt actctttgtc aaagagtaac attttcctaa ttgtgacatg
     2101 gctgtcacaa ttccaaatgc tctccgaggg tattcgctgt cacaagtgac aacgaaaaaa
     2161 ggagccgaaa gcgtgtcacg ctctcaactc cttttacgct ttattttgct tcacgcaaaa
     2221 taatcgctta tcgtaaattc acgtcattaa atattgacgt aaatttactc tcactcatct
     2281 gacttttctt attcggtatc actccgaata agccgtcaga ttcatcggga taatctcgtt
     2341 cgcttggcgc tgatttttca tcagcaagcc actcattatc cactgatttt tcgtgttgct
     2401 gatcgtcaga aatcgacgat agccaccgct caaaatcagc aaaagtttca aacccaaaac
     2461 gcttccgaag agccttgccc attgcagcat tttctttctc aatcaagcgt tttttctgtt
     2521 ggcgaagttt cgcttgttct ttttttaatt tttcttcttg cgctttaata tctgctaaag
     2581 tcattcccat aaactacatc tccttttctt tggaattaat aacggaaatt tggtttaact
     2641 gcataacatg actcattaat ttcaaccaaa attctttttc tgattcatca gttgtttgcg
     2701 ctaaatgctt atgaacaaat tcattagtca aaactgaaga ttttgctaaa tcagcattga
     2761 taaccgagtt gagaacatcc ataattcgtt caagcatttc actataatta tcttcatccg
     2821 gcaaattcaa ataatcagct aaccgttcat acatcaattc aattctatcg actgtttctt
     2881 ctctcaaatc aattttatcc atgtcacacc tcacttccag aattgccacc attttcgatt
     2941 ttttaattct tgattttcta gtaacaactg atcgtttttt tgttgctgaa gcagggtcaa
     3001 tttttgttga tcacgaatca tttctgttaa ttcagaaata tgtttgtcct tctcagctaa
     3061 ttgatcacgc aaaaactcat tctctttttc taaaggaaaa tcactgcctg tgttattgac
     3121 cggtaattga ccagtcaatt gatcactttt tgaccgataa attgaccgaa caacaccaat
     3181 caactcttta tctagcatga taatgccatt ttctttatac tgagcaaggt caaaccctga
     3241 attgaccagt tgattgcgca ctgtattttt cgatacaccc aattcgtcag caagattttt
     3301 tatggtcatc ttttctgaca aatcagtcat tagcttctcc actactttca gctaccgcat
     3361 aaatatattt aacggcgtgc ttagggataa taatcggaga gtcaatttta ttatttttca
     3421 aaatcaattc atattgcgtt acatcaagta acttaccgga catttttttg ctatccaaaa
     3481 aaacaaccac aacgtaatca cctgttaaat ttaccaacca atttttctta ccaaaatact
     3541 tttgtttttc tgtcattttt cttcctcctc tgttacgtct agccctagaa gccatgtgtc
     3601 taattctttt tttaaatcgt ctgccgattc cgcaaaggtt tctcgcaaat gattcgtcaa
     3661 ttcatcattt tgactttctt gcttcaacaa atccctgtat tttttctttg tattcttagc
     3721 tagatttccc cactcagaat gatcataaag caaacctttc accataatcc gttcagtaac
     3781 tttagtattt ggcggaaaat aatgtggact agcaaagcta tccaaacttt catgaaccat
     3841 ctcatgccaa tcagttgctt ttccccttcg taactgcaat tctagtcgcc accatgtcgt
     3901 tatttccggc ggaacaattt tacgttttct ttcttgttcc agttttttat tatacatccg
     3961 aatttgtcgt tcactggcac gactacccca atatgccgtt tctaacttac cagaacgccc
     4021 ataaattggt ttaaaagaca caggatcaac tacccgatat tgtgtaataa actcatcagg
     4081 tacatcgata atgtcgcaag caatatccgc acgtgaaaag tgaggttcga taaacaaatc
     4141 atgaataaaa tttttcatgg aactagctaa gaattgacta atcttattcg gat
//
LOCUS       DACDTO010000141         3999 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDTO010000141 DACDTO010000000
VERSION     DACDTO010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.144"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(109..1614)
                     /locus_tag="IWK70_002772"
     CDS             complement(109..1614)
                     /locus_tag="IWK70_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9615253.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1611..2297)
                     /locus_tag="IWK70_002773"
     CDS             complement(1611..2297)
                     /locus_tag="IWK70_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9615254.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2490..3911)
                     /gene="gndA"
                     /locus_tag="IWK70_002774"
     CDS             complement(2490..3911)
                     /gene="gndA"
                     /locus_tag="IWK70_002774"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9615255.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1099 a          850 c          687 g         1363 t
ORIGIN      
        1 taattgttcg ctagtttttt tataatgaca tccctgaatt tctcacttgc ttgtattttg
       61 taccttattc tttgttggat atataaaaaa acaagtaaag cacatgcttt acttgtcttc
      121 tgcctttgcg ataggaataa agattctaaa aattgtccct tgccctacta cactttctgc
      181 taagatacgt cctttatagt tttcaaccag ttgtttagcg atagacaacc ccagaccatt
      241 tccacccttc gttctagctc ttgctttatc cactcgataa aaacgattga agatcttatt
      301 aaggtcttct ttgggtattc cttcgccaaa gtcctgaatc gcgatctcaa attcattcag
      361 agtggaagaa atagagatat ggacttcttt tcgtgtagtt gaatacttta ctgcgttatc
      421 caatatgata atgattaatt gttcaaagtg attacgataa atttgcaggg tcacttcttt
      481 tgtcaaatca tcatctaaag tgatcacata gtctggatat aatatcttga aattattata
      541 aacttgatag gtgacttctt ttcctttagt tgtttcattc gcataatgaa catctacttg
      601 ttctgctctt gataagtcta gcatctcttg aactaagctt ttcatacggc tgatttcttg
      661 catgctggcg cttaatgatt cttctaatat ctcaggatca tctttccccc aacgatttag
      721 cagactaaga tgcccttcaa taattgcaac aggtgtcctt aactcatgag atacgtcttc
      781 cacgaattgc tcttgttgtt caatatacaa acgcatacgg tcaagcatct cattgaatat
      841 ttcagctaaa tctgccaatt catcatttgt gtcgatttca ggcatgtgga tatctgattg
      901 cggatcttta cgtatagtat ccattgtatc tctgagtacc ttgagtggtt ttaagaaata
      961 agaagataaa aagaaaccta aaatactact taagatcaaa gaaacaactt ctaaaacaat
     1021 aagagtcaat aatagcttat ttcgaatatc atagaaagaa gataattcat agaagctttg
     1081 cgcatagccg atcttttctc ttgtaccttt agaatatata ggctggatag ataaaaagcc
     1141 agtttttcct tctagtgtaa cgatcgttgg aactttacgt gtagtctgaa tcaggtttcg
     1201 actgttttca tgggttttaa aaatcaagtt ctcgtccaaa tcataaacat aaagatccat
     1261 tgatggctgc cctagctcag ctataaagga atcgatcttc attaagtttc cttcgaccag
     1321 tgaagtatga tctacgacct catctccgtc atttgtcgct ttcgtcagat tacgatacgt
     1381 atttactaat gtcagttctt catccgagtt agccaaacga gaagctactt ctgaaatcgt
     1441 acgctctaca ttgtttcttt cttttgccac aatcagattg atagatgatt tataggtaat
     1501 gacagcgaaa attgtaaaca caacgaatat gaagaaagaa ctcgcaaagg cccacttgat
     1561 tgtcaaggag ggccctttca gttctctttt ttccgatttt ttcggcttta tcacgagcgc
     1621 atcacataac ctgttccgcg tacagtttgg atatagcttt cctccccggg aacgtcaatt
     1681 ttgttgcgaa gataacgaat atatacgtcc actacgtttg tttcaacttc tgtttcgtat
     1741 ccccacactt tatttagtaa aacgtcacgt gctaatacca cgttcacgtt ttccattaaa
     1801 gtaagcagca actcgtattc tctttttgtt agttcaatta cttcatttcc gcgacgtacg
     1861 acacggtttt ctttttcgat agtcaaatcg cgataagtaa tagtagtttg tttagccaca
     1921 tttttatcgc cttcaatatc gattcgacgc aacaatgcac gaagacgtgc taataattct
     1981 tcgatagcaa atggtttgac gatgtagtca tctgctccgt ggtcaagtcc agatacacga
     2041 tcgatcacag aatctcttgc agtcatcata atgattggtg tatttttgac ttggcgtaca
     2101 cgtctgcaga cttctaaacc gttcaattct gggagcatca gatccaacaa tatggcatcc
     2161 cactcattat ttaatgccgc atctaaacca gttcggccgt tgtagtggac ttctgcgtta
     2221 tacccttcat gcttcaactc tagctcgaca aatcgtgcca agttcttttc atcttcaatg
     2281 attagaatat tgctcattta ttattttatc ctttctattc tactggcttt ttaaaaactc
     2341 tatcattata ccgataatat gcttaaaaat ctatagtcat tttatcatgg atagcaattt
     2401 taaaaaagag ctgaagtcta ctcataacaa tcgacttcag ctctttcgtc ctctttttat
     2461 tttctcattt cctaacagcc gaaagactat tattcttcat ggtaccatga gtaatggaag
     2521 attccttctt tgtcgacacg ctcataggta tgtgcgccaa agtaatcgcg ttgggcttgg
     2581 attatatttg caggcagacg ttctgctctg tatgaatcga agtaagcgat ggctgatgag
     2641 aaagtcggta caggtacgcc agcttggaca gccaaggcaa cgacgtctct gactgattgc
     2701 tgatattttt tcgtgatttc aacgaagtaa tcatctagca ataaattttc gatctcagga
     2761 tttttgtcat aggcatccgt aattttttgc aagaatcgag ctcggatgat acatccagcg
     2821 cgccagattt ttgcgatttc accaaatggc aagtcccaat caaattcttt tgaagcgaca
     2881 cgtaattgag caaatccttg ggcataactc atgattttac tgaaatacaa cgcttctcgg
     2941 attttttcga ccaattcttt tttgtctccc gtgaactcaa aatcgttcgt acgagacaaa
     3001 atcttgcttg cttgtacgcg ttcttcttta taagcagaaa tatagcgggc aaatacagat
     3061 tcagtgatca atggaagtgg cacacctaag tcaagcgcgc tttggcttgt ccatttacct
     3121 gttcctttat tcccagctgc atcaaggatc acatctacta ctggtttacc tgttccttca
     3181 tcgtctttac gtgtcagaat gtcagcagtg atttctatca aatagctgtc taattcgcct
     3241 tgattccatt ctttaaagat atcagccatt tcttctactg aaaggtttaa aatattcttc
     3301 attaagtcat aagattcagc aatcagttgc atatctccat actcaatacc gttgtgtacc
     3361 attttcacat aatgtccggc tccatttggg ccaatatatg tgacacatgg tgctccgtct
     3421 tctgcttttg cagaaatttg ctctaagatt ggtgcaacta gcgcataggc ttctttttgt
     3481 ccaccaggca tgattgaagg tccttttaaa gctccttctt caccaccgga aacgcctgta
     3541 ccgataaagt tgatgccaga gttggctaac tcttcattac gacgcatcgt atctttaaag
     3601 aatgtatttc ctccgtcaat caaaatatcg cctttatcaa ggtgaggaag taactcttgg
     3661 attgttgcat ctgttgccgg tcctgctttg accattagca agatccgacg tggtttctcg
     3721 atagattcta caaattcttc gattgtatat gttgctttga aatttttatc tgggtgttct
     3781 tctactacag cggttgtttt agcccctgta cgattgaata aagccactga atagccgcgg
     3841 ctttctatgt tcaaggctag atttttaccc ataacggcca taccgacaac gccgatttgt
     3901 tgttttgaca tatgaactcc tcctacatac gggatttctc cctaatctct tacattatag
     3961 cttaacacgg ctacattgca aactaaagaa caacatatt
//
LOCUS       DACDTO010000142         3894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDTO010000142 DACDTO010000000
VERSION     DACDTO010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.145"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            110..580
                     /locus_tag="IWK70_002775"
     CDS             110..580
                     /locus_tag="IWK70_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9615256.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            665..1273
                     /locus_tag="IWK70_002776"
     CDS             665..1273
                     /locus_tag="IWK70_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9615257.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            1407..1607
                     /locus_tag="IWK70_002777"
     CDS             1407..1607
                     /locus_tag="IWK70_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9615258.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            2361..3587
                     /locus_tag="IWK70_002778"
     CDS             2361..3587
                     /locus_tag="IWK70_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9615259.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(3695..>3894)
                     /locus_tag="IWK70_002779"
     CDS             complement(3695..>3894)
                     /locus_tag="IWK70_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9615260.1"
                     /translation="IWSALFFNLRNIFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLM
                     IPYYLWVLFATLLNYSIYFLN"
BASE COUNT         1373 a          564 c          788 g         1169 t
ORIGIN      
        1 cagagacaaa ggcaaaggat caaaaaaaat taattttttt aaataaagat gaaagttatt
       61 gaaatataga gtatgataaa taagaatttt taataaggta ggttggctca tgaagcagta
      121 tcgacagtta aaaggatatt tagaagcaaa aggatattgg gagatactag tctatctctt
      181 ttttggtggt ttggctacga ttgtaaattt tgttagtttt gctctcgcac gccaatattt
      241 tgatttaagt atggccttat ccaattctat ttcgtggttt tgttctgtat tgtttgcttt
      301 tgtaacgaac aaactttggg tattccattc aaaatcaccg aattttacac atgcacttat
      361 tgaatgcggt aagttcttct tctaccgtat cctttcttac ggattggaca tgggggcaat
      421 ggtattgttg atcaatgtta tgaacagcaa tgagtatgtg gcgaaaatca tcactcaaat
      481 tattgttatc cttgcgaact atattttcag caagttgttt atttttaaag aaactgaggt
      541 ttttgaagaa gaagtaggtc aaagtgaaaa agacgactag gcataacaat tgaagaattt
      601 ccttctcttt gttactcttt gtaatgtaga cattattaag aaaacattga ggaggaattt
      661 ttttatgact tacacattac cagatctacc ttatgcatat gacgcattgg aaccttatat
      721 tgacgaagaa acgatgcatc tgcatcatga taagcatcac aatacttatg tgacgaattt
      781 aaattcagca attgagaaat acccagaatt aggcgaaaaa acaatagaag aattattatc
      841 tgatatggac gctattccaa cagatatcaa gacagctgta cgtaacaatg gtggcggaca
      901 tgctaaccat tcatttttct gggaaatcat ggcaccaaat gctggtggcg aacctacagg
      961 agaaataaaa gaagcgatta atgaagcttt tggtgatttt tcttctttta aagaagaatt
     1021 caaaaaagca gccgctggac gatttggttc tggatgggct tggcttgtaa tggaaaatgg
     1081 aaaattagct attacctcta ctgcaaatca agattctcca ttgatggaag gaaagacacc
     1141 aattctaggt ttggacgttt gggaacatgc ttattatttg aaatataaaa atgtccgacc
     1201 agattatatt gcagcattct ggaatgttat taattgggat gaagtaaaca aacgtttcga
     1261 agcagcaaaa taaccatttc ctccgtcata tcagtcttaa aaataatttg gaaaataaaa
     1321 gagcgaaagc ttgctagcgt tttctaaatt gggtataatg ttcttgcaag gttacttgca
     1381 agaacttttt tggaggatat acatttatgg aacacggaac ggttaaatgg ttcaacaatg
     1441 aaaaaggatt tgggtttatt acagtagacg gcggagatga cgtatttgtt catttctcag
     1501 caattcaagg agacggcttc aagagcctag aagaaggtca agaagtagaa ttcacgattg
     1561 ttgaaggtgc acgcggacct caagctgctg aagtgactaa attataataa tttgttgaat
     1621 aaagacacga cttcttagag agtcgtgtct ttttgtctat ttatgaagtt tttagatttt
     1681 ttggctaagt aaatgcattt gttcacttga catgtcctaa ctaattatca agcaaggaac
     1741 attttcttcg ttagtaatgg attttaaaga aatttcagtt tttggatgtt gaataaaaga
     1801 ccatttgtga aaccactagt atacatactc attaccatgt caattctatt actttaaaag
     1861 aaaatcgttt tactttgaag aggagcatat ctatccatta aaagatgatg gtaatgcaat
     1921 cactagttcg agtcatttta agagccatgc agtgagctgg aaataaaacg aatagaagat
     1981 gggtttaaca aaaggaccag tcataggaag aaattgtcgt gaaatcgaat gataatctga
     2041 acggtaggca aagactgtta aaaagatggg aaaggtatgt gcgatcattt tactgcacga
     2101 ttatttggaa aataccttgt tgttcgcttt ttctggaaaa aatacataaa aaaagataaa
     2161 acgattgcga actgcgtgta ttcggtcttt cgatcttttt cacggtatgg tatgatagtt
     2221 aagaattttg agaaatttta aaaaataata atcgttacac aaaaatgttt tttaaagttt
     2281 tcttatgatt aaaaagtgaa tttgctgatt gaacagttat atgagacaat attttagaaa
     2341 atgtgaaaca gaggtgtatt atgaagcgat ggcaaaaagt tctattaacc ttgttagggg
     2401 tattcgtttt gctgattgcc ggattatcag catacggaat cagatttatg ggagaagcga
     2461 gccaaacagt aaacaaaatt tctaaaaact ctaatcgaat atcctccaaa cgttctgaga
     2521 gagtaagcat tgatgacaaa gaaccttttt cggttttgct attaggttta gataccggtg
     2581 gattaggaag aacggaacaa ggacgttctg atacgatgat ggtagtaaca gttaatccga
     2641 aacaaaaaaa atcaacgatc atcagtttgg accgagatat ttatacgaat atcgtaggtt
     2701 atggaacggt agataaatta aatcatgcct acgcatttgg tggtgtcgaa atggccatgg
     2761 attcaattga gcaactgctc gatattccta ttgatcatta tgtaacgatc aatttggatg
     2821 gcatggaaga tttgatcaat gcggtcggtg gcatcaaagt gaataataaa attgacttta
     2881 ctttggatgg tgtccatgta ccagtaggtg aacaaacatt gaatggagaa aaaggtttag
     2941 cctattctcg aatgcgacac gaagatccag aaggcgatat cggtcgacaa cgtcgtcagc
     3001 gggaagtagt gacgaaaatc gtcaataaag ttttaagttt agatggcgta agcaattata
     3061 gaaaaatttt aaaagcagtt gagaacaatg tgacgacgga cttagattgg gatgacatgc
     3121 ttgatgtagc gacgaactac accccggcgt ttgaaacaat caaacaagac cagttgcaag
     3181 gcgaaggaca aatgatcaat gatatctact atcaaatttt aggaaaaaat gatcttcttt
     3241 cgatccaaaa tgaactgaag aaacaattaa atataaaaac aagcgataca ttacctaatt
     3301 tgaaaaatga gaatgcttca atgatgttct ttgatgattc ggaagatggc gattcgacaa
     3361 acggtgatgg atcgaatagt gaagggtaca ccgattattc ttcctattca gaaaacacat
     3421 atgatcagta ccaagatccg aatacctatt atcagcaaac agaagataca agtcagtact
     3481 atacaccaag tcaagatact caaagttatg atccttacgc tggacaggaa caaaatggtt
     3541 atggttatga ccagaataat gagtatggaa caacaagtgg gtactagaaa aaaatagttt
     3601 ggttaataaa gagtgccacc taaattagac aaaaaatcta acttaggtgg cactttttct
     3661 atgtgttaaa ttacaggacg tctcttttta gcgcttagtt taaaaaataa attgaataat
     3721 tcaatagagt tgcgaatagt acccataaat aataaggaat catgagccac attgtcaggc
     3781 gatgatgcag ccacaactga tacataagca ctgacaagaa aataactaaa agtgtaatat
     3841 cgattgctgc aatgaaaata tttcttaaat taaaaaatag agcagaccat ataa
//
LOCUS       DACDTO010000143         3782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDTO010000143 DACDTO010000000
VERSION     DACDTO010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.146"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..115)
                     /locus_tag="IWK70_002780"
     CDS             complement(<1..115)
                     /locus_tag="IWK70_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9615261.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="IWK70_002781"
     CDS             246..698
                     /locus_tag="IWK70_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615262.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="IWK70_002782"
     CDS             712..1401
                     /locus_tag="IWK70_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615263.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..>3782
                     /locus_tag="IWK70_002783"
     CDS             1526..>3782
                     /locus_tag="IWK70_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9615264.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDF"
BASE COUNT         1277 a          510 c          776 g         1219 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 ta
//
LOCUS       DACDTO010000144         3712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDTO010000144 DACDTO010000000
VERSION     DACDTO010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.147"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWK70_002784"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWK70_002784"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9615265.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IWK70_002785"
     CDS             complement(2260..2511)
                     /locus_tag="IWK70_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9615266.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IWK70_002786"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IWK70_002786"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9615267.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IWK70_002787"
     CDS             3461..3679
                     /locus_tag="IWK70_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615268.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DACDTO010000145         3621 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDTO010000145 DACDTO010000000
VERSION     DACDTO010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.148"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            372..1412
                     /locus_tag="IWK70_002788"
     CDS             372..1412
                     /locus_tag="IWK70_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9615269.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2236..2568
                     /locus_tag="IWK70_002789"
     CDS             2236..2568
                     /locus_tag="IWK70_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9615270.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            3187..3528
                     /locus_tag="IWK70_002790"
     CDS             3187..3528
                     /locus_tag="IWK70_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9615271.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1343 a          475 c          672 g         1131 t
ORIGIN      
        1 aagaataata taatatatac tactgaaata aggtagtata tattactagt aatggtagta
       61 tgttttatat gcataaggta gttcttaatt tattttttgt tttgacactg gtagtactta
      121 attatcttta attttaaaac taaattttat tcatacgttt ttctggtagt atttaattat
      181 aaataatatt tatttaaagt agttcttaat gactttaaag gtagtacata attcatttta
      241 tttctgattt taggtagtat ttaattaaaa aacagtttta atgcgtttgc attttttatt
      301 tatctttgct attttcgaaa agagtaggta attttacata aataaaacaa cgtgaaagta
      361 gaggatctac aatgactgat tttaaatttt ttaaggcgga tagagtttac aacgaattat
      421 tttatcaatt tccaaaagtc tttattgttt ctgacgaata caaaaaaatg aaagattcaa
      481 ctaagattgc ctatatgctt ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa
      541 tcgatgaaga aggtaatgtt tattttactt atacgacaaa tgaactatgt agagtattaa
      601 actgccaaaa acaaaaagcg atagcaatca aaaaagagtt ggaatccttt ggtttattat
      661 tacaaaagca gatgggattt aacaaacagt tagggaaaaa taatcctaat agactatatc
      721 tagcagaatt aaaagtctca gaaaatgata tctacttact cgaaaaattt gatagagaga
      781 atagggaaaa cgttgataaa tcagagggta tgaaaatcat acccaccctc gacgaaaaat
      841 cagacgctga atcccttggg gctcaagagg gtatgaaaat cataccgtgc caaaacgttg
      901 ataaatcaga gggtatgaaa atcataccag aacttaataa taatatatta gacactaata
      961 gacacaatat agacactgaa aaagaccgcc tacaagatca attgttgtta gacaattttg
     1021 agacaattat gacaaacgac agcattgcta cgtttgtccc tgaacgagta ttaaatttga
     1081 taaaaacatt ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact attcataatg
     1141 caaagaaaaa agctgaaata gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt
     1201 attatgtcaa tgcagaacaa gaattataca cgacactgtt aaaagcctat caaaaattaa
     1261 aaaccgaaaa agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa aattggttta
     1321 tcgaaaaacc aatagctgct aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc
     1381 caagcactat tacgaaagac tggttagagt gaaactttac tgacaaagtg attgttgctt
     1441 catggagtaa cattaaaatt aaaaattttt tattattcta attatattac gaatattaca
     1501 aaataatagc tattttaata gaatttataa gttaaaatat tatgataaaa atcacaaacg
     1561 tagcctttta cgtcataagt gttaaaagaa ataattaatt tcaaatatag aaaagggaac
     1621 gtatgtttgc atactccttt tttttgagtt ataataatac gcgaagagag ataatttcat
     1681 tatttaaaca agctaaaaat cttgatatta cttcttttca ggcaatacga atattattcg
     1741 cttacatttt ttgtataaat ttaattgtct gctatgcttg tgatgaattg attttcgttc
     1801 tgcacttttg gatgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggcggtatga
     1861 actcggaagg ctggtaacct tcgtcagcgt tcatacacgg cattaagatt tggcccttaa
     1921 tgcgttaacc tttcagtgtt acggtattac ccgcaatgga tttagtataa cagatattca
     1981 aaagtgatac cacttttttt ggctgtgtta aggttaaaaa tacgtgatat tacgatgaat
     2041 ctatgcctaa gcacaatgtt ataaaaatgt ccgtgtggct ctttgccgta tgacgaacgc
     2101 tgaaattaga gggaacgcat gtttggatgc gttccctctt ttttgtttgt catgaacgaa
     2161 aatcaattct ctatttttga aaaataacaa gaaaaacttg ttaaaaatat ttaaagaaat
     2221 tgggtggttt attttatgtc atatacagga attttgagtc tcaaagatat ttgtcactac
     2281 ggtaaacggt gtaccgctac agagaaaatc acgaagaaac tatcaactgg ccaaagtaaa
     2341 accgttgtgc agtgtaagaa atatataatc cagaaagaca aagtctcaga ggaaatgatt
     2401 tattacattg ggaaacaaaa gcaaataatt ttaaaagatc ctatcccctt aaaagagcta
     2461 tatccaacaa tcaaacatgt ttatgaccaa aacggtgtac tgattggtcg aagaaaaaat
     2521 ggagtcttgc gttgtaccgc aaaagggatg gggcgtttga tcggctgaat tgatcattct
     2581 cataacttgt attttatcgt ttttcgcttg cttgaaaata ttgtcgagac ttctaaagga
     2641 acttagagtc actagaaaaa taaggagtag acttgatcta tcaacaattc ccttcgggga
     2701 gtgacggggt aggttttaac gattggtaac gtaaaaaatt ggtcaatcga gtaaatgcgc
     2761 ttgtttggat agtggtgaaa tgaatttgtg taaataataa gcgcagagta aacaagaggg
     2821 aatttagaag ttagacaagt tagaatagcg tctagattaa tcatgagcag ctactaaggg
     2881 atctagactt tgacgcaaac tcatggaata aggagtaggt cttctcgtaa tggggaggac
     2941 gcacaagaaa ttgtgtcgtg tgctgcagta catgtagata cttgcgagga cgttgattat
     3001 tggatcaatc gagcatttac cgctgtctac tagggtttct cgtttcggac gccataaacg
     3061 agtcgaaatt ttttatgtat ttttaagtaa acgaaaaacg ataaaatatg aacaagtcct
     3121 gaataaagtg aaaaaattga tataaaacat agagaaaatg aaatttacgt aatggaggaa
     3181 tgacaaatga aaaattatga tccgaatata cgattgggca cacatactat taaggtttct
     3241 ttccaacgtt gggattacaa aggatttgtg acgtttagaa gaggcggaaa ttgtaaagga
     3301 ttagatgttt tagcattaga cgaggatgat ttatacgatc aaacattgac tgataatcca
     3361 attggattcg gcttgctacc tgaagatgat gaaggtaatg aatggtttaa aatgacgctt
     3421 atgaatgata atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat
     3481 attgtcagct ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa
     3541 taaattttac agataaaggc ttagaaggag tcacgatgat taataaaaag gtagtattac
     3601 cttaggcatt gagtaaggaa t
//
LOCUS       DACDTO010000146         3620 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.149, whole genome shotgun sequence.
ACCESSION   DACDTO010000146 DACDTO010000000
VERSION     DACDTO010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.149"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..2232)
                     /locus_tag="IWK70_002791"
     CDS             complement(<1..2232)
                     /locus_tag="IWK70_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615272.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="IWK70_002792"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="IWK70_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1041 a          787 c          559 g         1233 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg aatccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacact tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 accaaataac aaactaacta tccatcaata acgtgtttta ttttttaaaa tttctataaa
     3541 aaaacaatca ttcttaaaaa gaatatataa aaataacaat aacctttgtg atttttcttg
     3601 tttgaagaat gttagaagaa
//
LOCUS       DACDTO010000147         3461 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.150, whole genome shotgun sequence.
ACCESSION   DACDTO010000147 DACDTO010000000
VERSION     DACDTO010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.150"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..652
                     /locus_tag="IWK70_002793"
     CDS             <1..652
                     /locus_tag="IWK70_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9615273.1"
                     /translation="SNIGIILSVIWMSTNNAWVPWFYGEMSKNNEKKVKKVSNYYLVV
                     FTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVPITLGYFFQFLYSLPVNAEFYEKKTS
                     FIAIGTVASAIVNILLNMIFIPRLGIIAAGYTTVFTYFLLFIFHYNIAKKITNKQLFD
                     TKKICIIIIFVSIFSLILHILINQMLIRYVLTSLLIISLVIYFLKIKNRIIDDYNS"
     gene            665..1672
                     /locus_tag="IWK70_002794"
     CDS             665..1672
                     /locus_tag="IWK70_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9615274.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1684..2688
                     /gene="neuB"
                     /locus_tag="IWK70_002795"
     CDS             1684..2688
                     /gene="neuB"
                     /locus_tag="IWK70_002795"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9615275.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            2685..>3461
                     /gene="neuC"
                     /locus_tag="IWK70_002796"
     CDS             2685..>3461
                     /gene="neuC"
                     /locus_tag="IWK70_002796"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9615276.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLK"
BASE COUNT         1266 a          435 c          645 g         1115 t
ORIGIN      
        1 tagtaacata ggtattattc taagcgtaat atggatgtct actaataacg cctgggttcc
       61 atggttctat ggtgagatga gtaaaaataa tgaaaaaaaa gtgaagaaag ttagtaacta
      121 ttatttagta gtctttactt tgattacgat gattgtaatg gtagtttcga ttgatttagc
      181 aaaatttatg gctccgaaag actattattc tgggttatct ttagtggttc cgataacctt
      241 aggctatttc tttcagtttt tatatagttt accagttaat gctgaatttt atgagaaaaa
      301 aacatcgttt attgcgattg gtacagtagc aagtgctata gttaatatac tattaaacat
      361 gattttcata cctaggttag gaataattgc tgctggatac acaaccgtat ttacgtattt
      421 tttacttttt atttttcatt ataatatcgc taaaaaaatt acgaataaac aattatttga
      481 cacaaaaaaa atatgtatca taataatttt tgtatcaatt ttttctttga tacttcatat
      541 tttaatcaat caaatgttga ttcgttacgt tttgacatca ttattgataa ttagtttagt
      601 tatatatttt ttaaagatta agaatcgaat tatagatgac tataattcat aggaggagat
      661 ataagtgttt aaaggaaaaa caatattagt aactggcgga actggttcca ttggaagtga
      721 aattgttcgt cagcttttaa aatatgaacc aaaagctatc agaatttata gtagaggtga
      781 agaaaaacaa ttttatatgc aacaagagct ctcaaatcac actaatttga gatttttaat
      841 tggtgatatc agagactctc aaaggattga ttatgcatgt cgtgatgtgg atattatttt
      901 tcatgctgcc gcaatgaaac acgttccagc aagtgaatat aatcctatgg aagctgtaaa
      961 aacaaatgtg atgggaactc aaaatgtaat agatgcagcg atcaaaaata atgtaaaaca
     1021 ttttgtaggt atcagtacag ataaagtagt gagtcctact aacaccatgg gtgcgacaaa
     1081 gttattgagt gaaaaattaa taatagctgc caatcagtat aaaggaaaaa cagaaacaat
     1141 attttcttgt gtaaggtttg gaaatgtcat ggggtcaagt ggatctgtta ttcctctttt
     1201 tagtaaacaa ataaaaaaca ataaagatat tactttgaca gatgttgata tgacacgatt
     1261 tatgatgtca ataccggatg ctgcaagatt agttctgtct gctgctatga attcaaaatt
     1321 tggagaaaca tatgtcttaa aaatgccggc aatcaatatt ttaactttag ctcaatcatt
     1381 aataaaaata tataatgata cacatgatga aaaatataga ggtgaaataa aagttgtagg
     1441 tatcaggcca ggagaaaaat tatatgaaga gctaatgact acagacgaaa ttgaacgctc
     1501 atatgaaaat gataaaatgt atatcattcc accacactat catgctactg acgaactgta
     1561 caaagattat gaaaaaatta aaatcactgc atactcttca gatgcaacag aattaatgag
     1621 tggacaggaa tttattagaa tcgcaaaaga gaataatgtc gtgacttggt aaggagtttt
     1681 atgatgaaat cagaagtata tgtaattgca gaaattggtg taaaccataa tggaagtcta
     1741 gatttagctt tagaaagtat tgataaagct aaagaatgtg gagctaacgc ggttaaattt
     1801 caaacattta aaacgaataa gctagtttcc aaaaaggctc caatggcaaa atatcagaag
     1861 gataatctta aagaggacag ttctcaattt gaaatgctaa aaaaattaga attatctcaa
     1921 tctgatttta ttagaataaa agattattgt atacaaaata aaattgattt tttgtctact
     1981 ccttttgatg aagaaagtgc agactttcta aaacaaattg gtgtacaagg atttaaaatt
     2041 ggctcaggag atttaacaaa tttacctttt cttagaaaaa tagatatgta tgggttgcca
     2101 gttttattat ctacaggtat ggctgatatt catgaggtca gtgaggctgt agattgtttc
     2161 atagatagtc cagttactat tttacattgt acttcaaatt atccggctag ttctgaagat
     2221 attaatttat tggcgattaa cacattaaaa gaaatgttcg ggatacccgt tgggtactcc
     2281 gatcattcat tgggatatga tgttggtatt tgtgctgttg cgatgggtgc taaggtaatt
     2341 gagaaacatt ttaccttgga taaggaacta cctggtccag atcataaagc ttctttagat
     2401 cctcaggaat ttgcagattt tgtaaagcat attagaaaca ctgaaatatt tttaggtgat
     2461 ggtgttaagc gagcaatgcc ttctgaactt agtacaaaag aagtatcaag aaaaagccta
     2521 gtggtcactg agaatttaaa tgtgggagat attcttacag agaaatctct tacaataaaa
     2581 cgtcctggaa atggaatagc tcctaagttt ttatatcaat atattggcaa aaaagttaaa
     2641 agaccaataa gtgcagatag tgttctgctt gaggaagatg ttgaatgaaa aaaatactta
     2701 tagtaagtgg aacacgagct gattatggaa tatataaacc tgttgctgag aatttactac
     2761 aagataattg ggatgtatca tttgttgttt ctggaatgca tttatcccat aaatatggat
     2821 ttactaaaga attgatctta gctgacggat ttagaattag tggagaagtt caaagtttgt
     2881 ttttagagaa caataaggga aacatggctc ggtctgtggc attagagata aatggtttca
     2941 caaatatatt tgaacaaact agccctgatt ttgtattatt actaggggat agaggagaaa
     3001 tgttggcagc tgcgatagct tgtttgtatc ttggaatcaa aacagcacat attcacggag
     3061 gcgaagtgtc tggtactgta gatgaatcaa taagacatgc cataacgaaa atagcatctg
     3121 ttcattttgc tgctacggaa aaaagcaaag agcgtttaat aaaaatgggg gaagattctt
     3181 ggagagtttt tcccgtcgga gctcctagaa tagatactat tttaaataat agattaccta
     3241 gttttagaga tataaaagaa aaatatcatc taaaatttga aaagaaaaaa tacgttttgc
     3301 aagtatttca tccagttgta accgaactag aaaatataga aaaacaaatt ttagctttga
     3361 tcaattcaat ggaacaacaa tctcaaccaa ttatttgcat tttacctaat agtgatgctg
     3421 gtagcgaaat tatcagaaaa atgtaccaga aatatttaaa a
//
LOCUS       DACDTO010000148         3309 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDTO010000148 DACDTO010000000
VERSION     DACDTO010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.151"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            126..338
                     /locus_tag="IWK70_002797"
     CDS             126..338
                     /locus_tag="IWK70_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615277.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(616..2415)
                     /locus_tag="IWK70_002798"
     CDS             complement(616..2415)
                     /locus_tag="IWK70_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9615278.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2539..3135)
                     /locus_tag="IWK70_002799"
     CDS             complement(2539..3135)
                     /locus_tag="IWK70_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9615279.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1203 a          596 c          583 g          927 t
ORIGIN      
        1 aaaaagcaga aagatactca aaataaaaaa taaaaaaatg gataaaaacg ttttcaaata
       61 caaggtaatt tgttattata aaataaagaa aaaacaaaat aaaaataaag aaaagaggga
      121 atgaaatggt aaaagcagaa gtaggaagat cttatatatg caaaccgatc ggagtatctc
      181 atgaactaat cggctgtatt gaacgtacat atatcaacac ggctgtcgtg tatgttgaaa
      241 gttatgacca acgagactat caacttatca atgaaagcaa gtatcgcatg ctcgtaaaat
      301 tctgtgatat tagcgcacct gctgaattgg tttcttaaac agtatttttg agaattcgca
      361 agaactccct tataatataa ataaagacag aagggagcga gaagatatgc caaaaagaaa
      421 aagttcaggg aaaatgggaa ttattcttgc agtgatctct gcaattcctg ctattttagc
      481 tattgttcgt gcgatgaagg aatcaagaga aagttaagca cacattatga gcgtcaaaca
      541 aaaaacgcat atacctgcgg tggcaggtta tgcgtttttt gttgtataaa gacttttttc
      601 attaacgatt tttgattagc cgactcccaa aacaaaattt agcagtttat ctgaataagt
      661 gatttcttca tctgtttgtt tcacactttc atttagtagt cccatctcat cggttatgat
      721 accatccaca ccataaaaca tcatgcgact catagcatct gaatcgttga ttgtccatac
      781 atatacttct tttccgtctt ggtgggcaga attaatgaag ttacggttga tcgtactata
      841 ttccattgtc agaaaatcag caggcgtcac agggggccca acaatattga aaggtaagat
      901 gtagcccaca taaaagtttg gttctgcttc ttttaaatct gaaacggttt ggtacgtcaa
      961 actttgcaaa atatgatggt tctctaagat aactttgcgg tatttatgga caaatcgttc
     1021 gacaaggtca tccgaatctt ttttagaggt cttgatctca ataagtaatt tttggtttag
     1081 cttatgtgct ttttccaaat aagaatcaaa agaacaaaca ggggcttgca ctccgttttc
     1141 agtaactgta agatttgtca attctttcaa tgtaagatcc tgtggtgttc catttacccc
     1201 tgttaagtga cgaagatttg catcatgcat cactacgaaa tgaccatctt ttgtttcctg
     1261 aacatccatc tctacataat caggatgata gtctctgctt gttttttcaa gtgcttctaa
     1321 agtattttgc gccccatttt gggtactcac acctctgtga gagacagtca ctggttttgt
     1381 ctgaacagct gaagtcagat aatccatatt gtaaagacag acgccaatac cgaaaaaaac
     1441 tgcaaaaaga gtcaaaccag tgtttttcag cgcagagaaa cgcaagttgg gggcttgtgg
     1501 gataaaccac ttagggatct caggcaagaa gccttcgtca tccatgaaat caacaatgat
     1561 ataaaaaatc cctacggtag acatcacgat attgaataat aaaatccctt gtaaaagcgt
     1621 catagcaaac acagcgctga tcaatgcata gtctgggaaa aattgttcaa ccatactttg
     1681 tgccaaaata acaacgatat atcctaaaga agaaaggagt aaaatcgtac cgccaatcac
     1741 aataaattgg cccgtgatgg ccaaaagacg cgatttggtc aatgaccagc tttcccgaat
     1801 ggctgctcga aaaggacgat ctcgtagaat catttccggt aaagcaaaaa tcagtcgaat
     1861 cccaatatag ccaaggaaaa tataaacgag taaaaaggaa ctgacgatga tccaacgatt
     1921 agcaaaaata aaatccataa tgaaagcggg gatttttatt tttgacagta gatcagaatt
     1981 aaaacttaat ccgctgatcg gtagaatcaa taaaaaataa gcaaggaaaa acaaaaaagt
     2041 aatgggtctc actttcttca gctgaagtat cgtcaagtgt aagagttgct tcaaagagat
     2101 tggttctttc tttttaataa aaaaaacact catcaaaaga aaagtaaatt cgaaataaac
     2161 taacagtaaa atcgtcatca aaactacgat taacgagagt agaaccccag gatgttgaga
     2221 aaaaattgaa ggaatattgt cataagacaa atagtcaatt gcccctcggt gtaaaataaa
     2281 acgggtcgta ctgctcaata agggtaaaca aataaataaa ataaagccat gcatcagcaa
     2341 gacatcacgg aaataagctt gtgttccttt taaaaagtcc caagcattac ctaagctacg
     2401 tttcaaataa ttcatcctat cacctgctct tcacattgta gcgcaaatct aatgaaaaag
     2461 gaaagggtgc agtgtgtttt gcagaccctt tcccagattt aacattgtca atcaatgact
     2521 aattttacaa aataaatatt atccaagaag accacttaga tattgacctc cataaatcaa
     2581 ggccatacta tataagaaaa tcgacagtgg tttagcaatc aaaatgatta gtgtatatcg
     2641 tttcactgac atgtttgtca atccagccat caaacataac gcatcgtcag gagcaactgg
     2701 aagaaaaata gctagagcaa acagcttctc aaaacggttt tgattatcca gccagccaat
     2761 atatttgtta tatgtcttat cgctaatcat ggaaagtatg aatggctttc cataacgacg
     2821 ccctagcaga aaaatgataa tcgaaccaat gcagattcca atatagttat agataaatcc
     2881 agcataaggg ccaaatatca ggacaccagc tgcagtactg atccctccag gaatgattgg
     2941 aataactact tgcaggattt ggatgaagac aaaaataatc ggtcctaaaa tgatggaatc
     3001 acctactaat cctcttaacg cattcaaatc tttgaaaaca cctaaattaa taaaataaat
     3061 cgtcagcgcg attgataggc ctaatccgat aatggatatg aaatttatca gttttcgaga
     3121 ggcagcaata ttcattattc atcaactcct tagaggttct ttgttaacta tactttacta
     3181 gaaaagcaac taactgcgcc attttttgat atgacagaaa acaaacaatt ttgtaagttc
     3241 atcataagta taaaagattg atctcaaaaa aacatccaac taaagactta tttaataact
     3301 tgttttttt
//
LOCUS       DACDTO010000149         3264 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDTO010000149 DACDTO010000000
VERSION     DACDTO010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.152"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IWK70_002800"
     CDS             complement(<1..1097)
                     /locus_tag="IWK70_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9615280.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="IWK70_002801"
     CDS             complement(1227..2603)
                     /locus_tag="IWK70_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9615281.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="IWK70_002802"
     CDS             complement(2603..3259)
                     /locus_tag="IWK70_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9615282.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
BASE COUNT          994 a          613 c          442 g         1215 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attt
//
LOCUS       DACDTO010000150         3128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.154, whole genome shotgun sequence.
ACCESSION   DACDTO010000150 DACDTO010000000
VERSION     DACDTO010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.154"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            103..1347
                     /locus_tag="IWK70_002803"
                     /pseudo
     CDS             103..1347
                     /locus_tag="IWK70_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="IWK70_002804"
     CDS             1360..1992
                     /locus_tag="IWK70_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9615283.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="IWK70_002805"
     CDS             2197..2424
                     /locus_tag="IWK70_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9615284.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="IWK70_002806"
     CDS             2493..2759
                     /locus_tag="IWK70_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9615285.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="IWK70_002807"
     CDS             2749..3018
                     /locus_tag="IWK70_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9615286.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1101 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttt
//
LOCUS       DACDTO010000151         3037 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDTO010000151 DACDTO010000000
VERSION     DACDTO010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3037
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.155"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(254..868)
                     /locus_tag="IWK70_002808"
     CDS             complement(254..868)
                     /locus_tag="IWK70_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9615287.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1474..1851)
                     /locus_tag="IWK70_002809"
     CDS             complement(1474..1851)
                     /locus_tag="IWK70_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615288.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2151..3032)
                     /gene="galU"
                     /locus_tag="IWK70_002810"
     CDS             complement(2151..3032)
                     /gene="galU"
                     /locus_tag="IWK70_002810"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9615289.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          918 a          637 c          468 g         1014 t
ORIGIN      
        1 tttttccact actttttatt tttctgacac tacatattct tataactcat ttctcataac
       61 tgtgaaacaa cttataaaaa taacaaactt taaacaaaac aagcttaaaa tcaaagatga
      121 tccatcttaa aataactaaa acaaaaaaag tagtcgttgt taatcctcat taataatcgt
      181 tttctttttt tcaaacgctc catttttatg tggaacatta tttttttaag gaactttgtc
      241 aacatgtgga taactatttt ccacttgtgt cctggactag ctgttttgct gtaaaataca
      301 aaacttgctg cagcaaaaag atcgttgcaa gagaggctgt tccgcagatc gctggatcta
      361 gatttggcaa agtcaagact gcatcatgta aattggcgtg gttgtccaaa accaaatcag
      421 caaattcaaa taatcgcaaa ccactgctat gttgcgattt gatacttcgt gacaaatcaa
      481 atccagtaac aatgattaat ggatgaccat tttctttgat ccactgagct agttcgatga
      541 ttgccgggtt tcttccttca ttcgaaagaa gaagaaaaat gtcttctgat ttgacagaaa
      601 gctgatcaat aattgcctgt cctgcacctt ctagcgtttc atagacacct ccggctggat
      661 tttcaatcaa tatacttggt acaagatccg gggtttgatc ccaaaattca aatgctacac
      721 cacttagtgt gcggctagca tatatctgca atctaccacc ttgattaatt tgtttcgcga
      781 tccaagtgcc agcttgaagc atcagcggct cttctatcct taatttctct tccaattggt
      841 gataatagtc aaaataacat aaactcattt atctctcccg cctagctata cttgacatga
      901 atagaaacag ctgcctaatc taaggaagta cataaaaaaa cagtagttag acagtaacac
      961 atgtggtctt tgctgtttca aacacaaaaa aggcattgaa cgaatacgcc caataccttg
     1021 acatagcaaa taaaacaacg tgcagaccat ggaaaatatc tacatagggg tagaattttc
     1081 accctagatt aggtacaata aattaggtcg atgcactgca ttgactatgg caaaatgatc
     1141 ggtcagggtg tatgtgtgtg actggtcatt ccctgattct tagcgccaac taagggtcag
     1201 ggagcctttt tttattcagc tgtttctatc tgaatttatt gtaagtaatc tcttatccaa
     1261 gtgcaagcaa gaaaatacaa aaaaacattg gtatgattta tatatatata aataacataa
     1321 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
     1381 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
     1441 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
     1501 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
     1561 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
     1621 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
     1681 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
     1741 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
     1801 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
     1861 acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg
     1921 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
     1981 tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga
     2041 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
     2101 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
     2161 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
     2221 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
     2281 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
     2341 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
     2401 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
     2461 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
     2521 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
     2581 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
     2641 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
     2701 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
     2761 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
     2821 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
     2881 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
     2941 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
     3001 gcggcaggaa tgactgcttt tttgaccttc acttgct
//
LOCUS       DACDTO010000152         3013 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDTO010000152 DACDTO010000000
VERSION     DACDTO010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.157"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            177..359
                     /locus_tag="IWK70_002811"
     CDS             177..359
                     /locus_tag="IWK70_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAP9615290.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="IWK70_002812"
     CDS             462..746
                     /locus_tag="IWK70_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615291.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="IWK70_002813"
     CDS             762..998
                     /locus_tag="IWK70_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615292.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="IWK70_002814"
     CDS             1020..1274
                     /locus_tag="IWK70_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615293.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="IWK70_002815"
     CDS             1356..1541
                     /locus_tag="IWK70_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP9615294.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="IWK70_002816"
     CDS             1608..2003
                     /locus_tag="IWK70_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9615295.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="IWK70_002817"
     CDS             1993..2370
                     /locus_tag="IWK70_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615296.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2371..2766
                     /locus_tag="IWK70_002818"
     CDS             2371..2766
                     /locus_tag="IWK70_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615297.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          925 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc ttt
//
LOCUS       DACDTO010000153         3000 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDTO010000153 DACDTO010000000
VERSION     DACDTO010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.158"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            624..>3000
                     /gene="esp"
                     /locus_tag="IWK70_002819"
     CDS             624..>3000
                     /gene="esp"
                     /locus_tag="IWK70_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9615298.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKD"
BASE COUNT         1095 a          454 c          588 g          863 t
ORIGIN      
        1 caattaatta ttcatgtaga gtactagaca ctttaaatat ggacaattca attataaaga
       61 aaaagaatca ataatttaat gaggctgtga caaatgtcat tttttgtttc gctaaaaacg
      121 agtgaaaaaa tgttgataac tccaatcatt cgtttaagat tctaggaatc attcagaaat
      181 gctgtgaaac cgatacttta taattataaa gaaggtgatt tcaaattccg aagaaggaat
      241 ttgaagtcac ctttttactt atgtcacagt ctcattcagt aatgcgggat aaggctcctt
      301 attccgttat ttgaatactc tgaagtttaa tctaagaaag ggtaatattt tgaaaacatg
      361 cttcatcgca tagaaatgaa cttaatttca gaaagaatac atacatatct ttttatatgt
      421 tttcagctgc tgaatggtta aaaagcacaa atatccgaaa aaaacatgaa gaaatgtact
      481 ttttttgttg taatgttcat gaaattctct aagtaaatta gttaacataa ggttagtaag
      541 attcctattc tgtgaataat acgtatcaga tttatctttt attcttacta ggaaagtaat
      601 tattaaggga gttgatttga ataatggtta gcaagaataa taagagagta tttctagaaa
      661 aaacaaagaa acgagtattg aagtacagta tcaaaaaatt aagtgttggg gttgcatctg
      721 ttttgattgg tgttggtctt gtcttgggaa caactgaact agttaaagca caagatgaaa
      781 tttctccaag cagcccactt gaaacagcga taagctccgt acaagtaggt gacaaagtag
      841 cttcggggaa tacttttcag gagaatcctg gttatacgaa aaattataac ttttcggatt
      901 tacaatttag tcctcaagaa ctaactggag acacactaaa aggcaataca attggatttg
      961 aagtttacgg aaaacataat attgcagcat caactaaaaa ctgggaaatc cgtcttcaat
     1021 tagatgaacg attagctaaa tatgtagaaa aaattcaggt cgatccaaag aaaggaattg
     1081 gatcttctag acggaccttt gtaagaatta atgattcgct gggtagacct acaaatattt
     1141 ggaaggtcaa ttatattcgt gcaagtgatg gattatttgc tggggcagaa acaaccgata
     1201 cgcaaaccgc tcctaatggg gtaattacgt ttgaaaagag cctagatgaa atttttaaag
     1261 agataggcat agacaatctc aagaccgatc gtttaatgta tcgaatctac ttggtaagtc
     1321 atcaagacga tgataaaatt gtaccgggta ttgatagtac gggctacttt ttaacagatt
     1381 cagatgattt ctataatagc ttagatgtat ctgaaaataa tcctgaccag tttaaacatg
     1441 gatcagtaag tgcaaaatat gagaaaccta atactcaaac aaaagatggt tcaggatcca
     1501 caggtgctaa tggagctata atcttagatc ataagttaac gaaaaattat aatttctcat
     1561 attcatcttc cgcaaagggc acgccttggt atgctaatta caaaattgat gaacgattag
     1621 ttccatatgt agcgggtata cagatgcaca tggttcaggc tgacaaagta acatatgatg
     1681 tttcgtttga atccggaaca aaagtagctg atttagcaat cgagcgacgg gaaggtcatg
     1741 aaaattatgg cataggttca ataactaata atgatttaac taaacttatc gattttgcta
     1801 atgcaagtcc acgtccagtc gttattcgat atgttttaca attgactaag ccattagatg
     1861 aaatcttaga agatatgaaa gcaacagcac aagttgaaga aaataaacca tttggtgaag
     1921 atttcatctt tgattcttgg ttgtcggata cgaataaaaa attaatccag aacacttatg
     1981 gaacaggtta ttattatttg caagatattg atggtgatgg aaaccctgac gataaagaag
     2041 agagcggaga cacgaatcca tatatcggga aacctgaatt agaagaagta tatgatgttg
     2101 acacaacagt taaggggaaa gtattcatcc acgagttagc gggaacaggt cacaaagccc
     2161 aacttgttga taaagaaggt actgtattag cagaaaaaac tatcgctcca aatgaaaaag
     2221 atggggctcc aatttcagat actgtagaat ttgaatttac gggtgtagat tcaagtaaat
     2281 taatcgcgaa agatgaatta aaaatccaaa tcgtttctcc aggttttgat aaaccagaag
     2341 aaggttcaac cgttattaag gaatcaccaa aagcggttga taaacaaacc gtggtagttg
     2401 gatttaaacc agatgctaaa gaatcaattc ggaataataa aaacttacct gaagatgcag
     2461 agtattcatg gaaaacagag cctgatactt ctaacgttac tgatagtacg aaaggtattg
     2521 taactgttaa gatcggaaat cgaactttcg acgtggatgt agagtttgct gtaaaagctt
     2581 ctcaagctat ggaaaatgat gcaacatacg tacctataac aacaactcca gaaacgacag
     2641 ttcaaagtgg aaaacctaca tttgataaac cagatgttcc tttagctaaa gatgcctttt
     2701 caatttcaga tgtctataat aagtacttcg gtaatgcaag tgttgatgca aacactggta
     2761 ttgttacatt cactccagct aaaggtgtag gagaatcgga gccgattact ggaacaattc
     2821 ctattaaaat tgtttaccaa gatggttctg taggcacgac cgatttagca gtaactgtaa
     2881 gtaaagatat ttatgaaaat ccaggagaaa acattcctgc aggctaccac aaagtaacct
     2941 tcaccgcagg ggaaggaaca agtattgaaa gtggaacaac agtctttgca gtgaaagacg
//
LOCUS       DACDTO010000154         2948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDTO010000154 DACDTO010000000
VERSION     DACDTO010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.159"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1350
                     /locus_tag="IWK70_002820"
     CDS             <1..1350
                     /locus_tag="IWK70_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9615299.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="IWK70_002821"
     CDS             1484..2827
                     /locus_tag="IWK70_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9615300.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1171 a          398 c          561 g          818 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaat
//
LOCUS       DACDTO010000155         2933 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.160, whole genome shotgun sequence.
ACCESSION   DACDTO010000155 DACDTO010000000
VERSION     DACDTO010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.160"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(552..2165)
                     /locus_tag="IWK70_002822"
     CDS             complement(552..2165)
                     /locus_tag="IWK70_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9615301.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..>2933)
                     /locus_tag="IWK70_002823"
     CDS             complement(2306..>2933)
                     /locus_tag="IWK70_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9615302.1"
                     /translation="HITGQSFSIDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEIN
                     SFFILCQQMSIHLLSLLVTYCLQKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILY
                     SDELVDPIIRYLEEHLSENFLLEQLAQIFFISPASLKKHFRKVNGKSVISYFKELKME
                     KAKRLVLENELTYTEIADILGFHSIHHFSSAFKKYTGLSPSKYYLSLK"
BASE COUNT          941 a          524 c          511 g          957 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgt
//
LOCUS       DACDTO010000156         2927 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDTO010000156 DACDTO010000000
VERSION     DACDTO010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.161"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..811)
                     /locus_tag="IWK70_002824"
     CDS             complement(<1..811)
                     /locus_tag="IWK70_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615303.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFL"
     gene            complement(1525..1815)
                     /locus_tag="IWK70_002825"
     CDS             complement(1525..1815)
                     /locus_tag="IWK70_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615304.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(2023..2715)
                     /locus_tag="IWK70_002826"
     CDS             complement(2023..2715)
                     /locus_tag="IWK70_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9615305.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          902 a          528 c          409 g         1088 t
ORIGIN      
        1 ataaaaataa gatattcatg tctaaaaaaa acggctttcc aaatgatgaa atacctaaat
       61 ctaacttatt aagaaagttg aaatttttat attcaggaaa ccatttatta atttcatgtg
      121 cagcacgtaa tgcattccct ggagttgtca aagagaaaat taattctgct aagcttatta
      181 ttgaaaatac tgataactta taattatatc gtccattgac aatcaaatat aaactaacta
      241 tacttgttag aacaaaaaaa caggcgttga cttgttcttg attagttaaa aatatcaata
      301 aaggaatact tacccaatat atttttctat ttatttcttc tcctcttaag agttgaaaaa
      361 acggataaaa agttagtaaa ctaaatgcta ctggccactg atagttcaag gtggttgctc
      421 tccaaccggt ttcccccatt actgtcaatg gtattaaaca gtatatagag caaaccaata
      481 ataaattttt aacatttatt ttctcaaaaa catacttgca aagtaatact acaattccta
      541 acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag aacattaaga
      601 atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa gcacccaaat
      661 agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata gtcggcatat
      721 acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc agttctttgt
      781 tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata tttcctaata
      841 gagtataaca tagctttaag cattacttaa agataactta aaagttatct tttaaatact
      901 taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca aaaaaatttt
      961 cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta atgcagaatt
     1021 cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac gaacaagata
     1081 tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca ggacgttctc
     1141 gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac ctcttcgagt
     1201 tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc tgagactagt
     1261 aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact aatagggctt
     1321 cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa agtttagtga
     1381 ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt gtgagcgtat
     1441 gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag cggctaggac
     1501 atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga ccttttttct
     1561 tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca catgatagga
     1621 tctcacaaca aattgctttc tattattata ataacctgta acgactaact tcatcccttc
     1681 gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga ccccatttaa
     1741 actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat ctactgttcc
     1801 atattttttc tccataacct tcacctcaat accattatac gaacatacat tccctttgta
     1861 aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt aaaatcatat
     1921 tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca gtatagatta
     1981 ctgttgcttc tttactaatt taggtttact tgttaagaaa gattatttac gcaaatgttc
     2041 ttcaacaatt tgctgagatt tatcagctgt atcttccact tcatcttttg ctttttctaa
     2101 cttttgaata tattgtttca agcttgagtt ttcagcagtt gcttcgtcta atttcttttg
     2161 tgtatctgtt aactgagttt ctacatttgt tttttcttgt gtcagtgttg cgatttgttg
     2221 attcaaacca tttacagtat tttgtaattc tgaaagtttt gcttgtgctt cttgtagtct
     2281 tgctgtccaa tctgcattca gatgattgat ttcatcattt ttttggttga tctcatctag
     2341 tttgacattg atttcatttt gtttgctttg tagttgtgca tccttgctga ctaattgact
     2401 ttctaagttt tgtttttctg ctgccgattg atttttttgg ttttctaatt catttacttt
     2461 tgtttggtat tcttggacat ctgcctttga atcttgcaat gtctgttctt tttgtgctag
     2521 tcgagcggta agatctgtaa gcgcttgctc tttatctgtc aatgtatgat caagctgtgt
     2581 cagattctca ttgattgtat caatatctgt ctctccaccc cacaaaataa tttcatttgc
     2641 gtaccgcgtg cctgtgtagc caattagaca tagaaatgca acgacaatcc caccagtaat
     2701 aaatttcttt ttcatttgca tagctcctta atccaatact tttacatgct aaaagtaagt
     2761 gatctcaaac acaatgtcaa gcgtttttaa attacatgtg taattttaat ataaatacta
     2821 tcaaatactc atttaatcct ccttttttat ctatttaaaa aatctttttt atagagaaaa
     2881 atgcaggtta atactttctt aaataaatat ttatggtgaa caaaaac
//
LOCUS       DACDTO010000157         2864 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDTO010000157 DACDTO010000000
VERSION     DACDTO010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.162"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..527
                     /locus_tag="IWK70_002827"
     CDS             132..527
                     /locus_tag="IWK70_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615306.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            852..1469
                     /locus_tag="IWK70_002828"
     CDS             852..1469
                     /locus_tag="IWK70_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9615307.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            1510..1809
                     /locus_tag="IWK70_002829"
     CDS             1510..1809
                     /locus_tag="IWK70_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615308.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1843..2010
                     /locus_tag="IWK70_002830"
     CDS             1843..2010
                     /locus_tag="IWK70_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9615309.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(2055..2660)
                     /locus_tag="IWK70_002831"
     CDS             complement(2055..2660)
                     /locus_tag="IWK70_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9615310.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         1015 a          422 c          514 g          913 t
ORIGIN      
        1 attttctgaa aaaattattc aatttcaaga aaatagaagt aaacttaatt aagaatcgtt
       61 tactatttaa acagtgtcct tttccttttt aaatatatac gataaaataa gtatatgaat
      121 tgtaaaagga catgatgatt atggaagaaa tagaatggaa taatctgact caaaataata
      181 aaaataattc tggatgtgga attatcttta tgtttgttat agttttggga atagtagtac
      241 ttttaataac tgtcaagata gtagattact ttgcgaatac tcctgaagaa caaatacaga
      301 gaaaacttcc tgagtttgaa agagtaccac cttatgatcc aagagataat tcatttcaag
      361 taaaaaaatt aaaagagtct ccaaatgaaa tgtggtttga agttacagtt gaatcaagcg
      421 acttcaaaga aaaaaagaaa gaatacaatt atattgtaaa ggcaaaagta tcaaaaagat
      481 ttatttttaa ccctaaatta gaaatatata gtgttgtaat agaataatag tgttaaaaaa
      541 attgtaaaag ttaaagaagt caaattctga tgtgaaaaat aaaatatacc atggaagaca
      601 taaaagaaaa actggataac tagtttattt aatggcagac cgttaaggcc tgccattttt
      661 atgtatttta gcggaataaa tataatcatg cgggagtgta aacttactgt ttcaaaaagt
      721 atactttttg acatgccttg attttctttg tttatgtatg gcataataac cacataatac
      781 aatttgaaac gtttctgatt taatctgatt gttttgaaac aattttagtg aaaaaagggt
      841 agataaaaac gatgagaaaa tttttttata tccgggtcag tagttatgat caatcgatcg
      901 aacgacaact gatttccgcg aaggaattag gaatccctga agaatatata tttgtagaaa
      961 aagccagcgg caaagatttt aaacgccctg aatatcaatt aatgaaacgg atgtttcgaa
     1021 aaggagattt actctatcta caatcattag atagactagg cagaaataaa caaatgattt
     1081 tagaagaatg gcgagaatta actaaggaaa agcagattga tatttctgtg ttagacatgc
     1141 ctctgttaga tactgctagt tacaagcaac tagatgggtt agagacgctt atctctgact
     1201 taattctgca attactcagc tatatggccg aagacgagcg gaaacgaatt aatagccggc
     1261 aaagagaagg cgtagaagct gcgaaacaaa agggtgtaaa attcggcaga aagaaaattg
     1321 ctttacctcc tgaatttcct gcgatctatt ctgactggaa agcaggaaag ataactgcag
     1381 tctatgctat ggatcaattg ggcatgaaac gaaatacctt ttatcgacgg gtgaaagaat
     1441 atgaacatac cttgcaccac ttttcttgat agatataaca gctattagac gggaagggag
     1501 agtttgaaaa tggacttaac gaatcaaagg gtcgttttta ctggaacgct gcagcaaatg
     1561 acacgaaccc aagccatcgg tttggttcac tctttaaacg gccattgtca atcaagtgtt
     1621 acttcaaaaa caaatttcct ggtttgtggt cagtattcta agtcgttgtt tgatgattct
     1681 ctctatacaa aaaaggaaca aaccgcgcgg gacttgattg cgttaggtca tgagattacc
     1741 attttatcag aagtcgaatt ttatgcgtta attagtcaac agttgaaaga atggcaacgg
     1801 tatttataat cgttagtaaa atttacaagg aggaaaattt ttatgaagcg aaaatcttat
     1861 gatcgttggg gcattgaaat aactagtttt aatcgaaata ttgcttttca cgtggagtgt
     1921 gactgtgggg aattagcaaa aagtgtttat caaaaaaatt acttttactg tcctaaatgc
     1981 catcgccgat acggggtaca tggaacatag aaacgaaata gtgcggtaga aaagcgaatg
     2041 aatgtagtat atttctacaa atcattcgct ttctttattt tataaatagt tggtcgagag
     2101 atcccgtttt cttttgcaat ctctgcaact ggaatgcctt gctttaacat gtccaccacg
     2161 gttttaaaaa ctaagcgttt ttgcggatct gttgagttaa tagaatactc gattggtcgt
     2221 cccttatatc ggccggcttt ttgagccaat aatactcctt gccgctgacg ttcacgaatt
     2281 ttctcacgct cgttttgtgc aatataactt agcagtgata aaaacatatc gaacatagcg
     2341 gtatccagga gttcatttcc agtattaaaa tctaaaaact gtgcatctaa tatttttaac
     2401 gctacttttt tctgtttcat aaaagcaact gtatttttta tatcttcata atctcttcct
     2461 aaacgatcta gtgatgtaac gatgacgcaa tcaccttcac ggatataatt tagtaatttt
     2521 tgaaactcag gacgctcagt atctttacca ctggcttttt cttggaataa tttatcaatg
     2581 ttaagtcctt ccatcattgc caattgtcga tccaaatttt gatcttgtga agatactcga
     2641 atatagccaa tttttcccat aaaaaactcc tttttaaaag tgatataatt tacatgtaaa
     2701 atttacttgt agttttactg ctatcatagc atttttaaaa ataaatttta tgtagactta
     2761 aaatttactt catggagggt ttaaatggat gaaatcaaaa gtaacaaaaa agcatttggc
     2821 atttatttag gagaacctag tacagaacaa ttaaaccaat tttt
//
LOCUS       DACDTO010000158         2838 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDTO010000158 DACDTO010000000
VERSION     DACDTO010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.163"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            97..756
                     /locus_tag="IWK70_002832"
     CDS             97..756
                     /locus_tag="IWK70_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9615311.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            775..2358
                     /locus_tag="IWK70_002833"
     CDS             775..2358
                     /locus_tag="IWK70_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP9615312.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2493..>2838
                     /locus_tag="IWK70_002834"
     CDS             2493..>2838
                     /locus_tag="IWK70_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615313.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFI"
BASE COUNT         1088 a          307 c          542 g          901 t
ORIGIN      
        1 gtagacgatg ttatttttat aaaaagatat aatttattaa tggtcgataa ttatgaaagg
       61 aaaaatattc ttaaaaaaaa gagtatagga tgtcgtatga aaatagtcgc tatagtacca
      121 atgaagttaa ataacaaaag attgccacaa aaaaacacga aagcttttac caatggaaaa
      181 ccattgtgtt attatatact ttcaacactt ttaaaagtaa aacagattga taaagtgtat
      241 gtctattgta gtaacccaga tattaaagaa tttataccag aaggtgtaac atttttaaag
      301 agatctagtt ccttagatca ggatacaacg agtattacac aggtattagc cggtttcaca
      361 aaagaagtag aggctgatgt atatgtaatg acacacacaa cagcaccatt tattagcgaa
      421 aaatctattg aaaaaggact agatgccgtg ctgacaggag aatatgattc tgcatttgct
      481 gcaaaaaaat tgcaagattt cttatggaaa gacggtgaac catttaatta tgaattggac
      541 aatattccta gaacacagga tttgccgcct ttatacgaag aaactagtgg attttatatt
      601 tataatcgca atgtgatgac cgagttgcat agaagaattg ggaaaaaacc ttgcatagtt
      661 gaagtaggtg agattgagtc agttgatatt gatgaagccg aggattttat gattgctgat
      721 gcgatttata atcatcttat tttacaaggt agttaagaca gggggaaggt taggatgaac
      781 aatgtacaga ttttagattg cacacttaga gatgggggct attgtaacaa ttgggaattt
      841 ggatttgata atacaaaaaa aattatttct ggacttatag aatcaggaat tgagattata
      901 gaatgtgggt ttctcacaaa taaaacagta tacaataaaa atgttacaaa atatacgacc
      961 ttagaagaat tagcacagat aattcctgaa aaaagagaag gaaaattatt tgtagcaatg
     1021 atgaactatg gcgaatataa tatagaggat cttccagatt atgatggttc atctattgat
     1081 ggaattagga ttgcgtttca taaaaaaaat tgcaatgaag cgttgaaatt atgtcgtgag
     1141 ataaagaaaa aaggctactt agtttttgtt caagctatgg tatctctcaa ttatacagat
     1201 gaagagtttt tagcagtaat aaaaaatgtg aatcagttta aaccatacgc tttttatatt
     1261 gttgatagtt ttggaatgat gaaaggaaaa gatttaactc gtctttttta catgattgag
     1321 cataatctag aaaaagatat acagataggt tttcactcac ataataacat gcagcttgca
     1381 tattctaatg cacaaaaact tataaccgta caaacaaatc gaagcctgat tattgattct
     1441 tcggtgtacg gaatgggaag aggagcaggt aatcttaaca cagaactatt tgtagaatat
     1501 ttgaatgata atgcaggaaa aaaatatcaa ttaaaaccat tattatatat aattgatgaa
     1561 atactaaata gtttctatca gaagagttat tggggatatt cattaccaaa ctatatttca
     1621 gcagttcata atgctcatcc taattatgca agctatctgt catataagaa tactttaaca
     1681 attgaagcaa tgaatgaaat attcgaaatg atggatgaag aaaaaaaggt atcttatgat
     1741 aaacaatata tagaagattt atactacaaa tatatggaaa ctgggaaaat acaggaggaa
     1801 tatatggaag aactgagaaa atttctctca gacaaaaaaa ttctactgat tgcaccagga
     1861 aaaagttctg tagatgaggt agaaagaata aaagagtttg ttaccaaaga agaagtcgta
     1921 ataattagtg taaattttga atattctcat atagatatag attacgtatt tttaagtaat
     1981 ttaagacgtt ttcatgaatt accgaaagaa agcagaacta aatgtattgt tacatcaaat
     2041 atacaagcat cggatgtata tttacggacg agttataaaa agttgttatc tgaaagggaa
     2101 gttgtaagag acaatgcagg attaatggca attagatttt tagccgatat gaatgtggat
     2161 gaaatattct tagctggatt tgatggatat tcacataatg aagatgagaa ttatggtgag
     2221 caatccatgg aaattataac aaaatttgca ttattagatg caatgaacgt gcaaatgaga
     2281 gatgaattgt cagaattagc taaaaaagta aaggtgacat ttttaacgga tccacgttgg
     2341 gttcgaattg ataagtagta gtaataatgt ttttaaaatt ttgataaaga gatagtgaga
     2401 gaataaaaat gggtaaagtc ttaaataaaa aagaagaaaa atatttaagg gacgatgggg
     2461 cgagatgaaa ctctaatttt gaacttttaa gaatcatttc tatattgtgt attgttgctt
     2521 accatgctaa tttgcgttta aattataatg cagacggaat tatagtacat ggttcattaa
     2581 ctgctaacca gattttcttg aattatattg gtatttgggg tcagctagga gttaattgtt
     2641 ttatatttat ttcggcatat tttttaagtg agaaaagaaa cattcacttt gaaaatgttt
     2701 tgggaattgt attagaatgt attttttata gtgtattagt ttatggaatt ctttgtgcat
     2761 gtggaataga aacattttca ttgaacagct ttgtaaaaga aatattatca tttgctctag
     2821 gtagatattg gtttatta
//
LOCUS       DACDTO010000159         2830 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDTO010000159 DACDTO010000000
VERSION     DACDTO010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.164"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            802..1068
                     /locus_tag="IWK70_002835"
     CDS             802..1068
                     /locus_tag="IWK70_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615314.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1126..1638
                     /locus_tag="IWK70_002836"
     CDS             1126..1638
                     /locus_tag="IWK70_002836"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9615315.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1635..2672
                     /locus_tag="IWK70_002837"
     CDS             1635..2672
                     /locus_tag="IWK70_002837"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9615316.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1023 a          496 c          564 g          747 t
ORIGIN      
        1 tttattgatg accacgaaag tgcatggcga tatttgatgt tgatacttta tgcagtaccc
       61 tatgtattct tcgctatcat tctgtggctc atagcattgt tttttagagt atttaatgac
      121 ctttaggtag atttatatat aaaaagggct aaatacgcaa aataccccct taaaatgcaa
      181 aataaatgca aataaggggg taactcctta tgaaaaaaca actaatagta ttataacacg
      241 tttttatgcc gagaaaactt attggttgga atgggctatg tgttagctaa cttgttagcg
      301 agttggttgg acttgaattg ggattaatcc caagaaagta ccaactcaac aacacataaa
      361 gccctgtagg ttccgaccaa taaggaaatt ggaataaagc aacgaaagga gttgagtaaa
      421 atgagtgaga aaacgaagaa aatcattcga gtatcattag ctttttcaca agtagtagtg
      481 caatcattga tagtttatta tacttaccag caatataaat tatctaaaga gaattaagtt
      541 ttgattatgt aataagtttt ggtactaaaa aagacttgat ctaattagac caagtctttt
      601 gttagtgtta tattaataac aaaataaaaa taagaagtcg ctcactcccc gaccaaagtt
      661 tgtgagcaaa ataaacagtc gatgtttgtt tcttaattgt atatcttgat attaaacgat
      721 ttaagattat tcttcaagat atatattcgc aaatgtaatt ggaaaagcga cttcttaaac
      781 aaaattaagg agtcgctttt tatggcaaaa ataacgaaaa atgagcaaga aaatttggtg
      841 gaaaattaca caaaaaaacc ggcgtactct aatagccggt tagacgcaca tttcgtgtgc
      901 acttctaatc cgaaattgag ctttgacgct atgacgatag tcggaaacct taataaaaac
      961 agcgctaaaa agctatccga ttttatgagt ttagatccac aaattagact ttgggatata
     1021 cttcaaacaa aatttaaagc taaagcacta caagaccccc tatgttaggc tagttgtcgt
     1081 ataggataat aagttataat aaagactata cggaggatga ttaccatgac aaataaaaac
     1141 aatgaacgtt actctaccga actgaaagcg acagccatca agaaaatgat gccacccgaa
     1201 aataggggga ttcgccaatt aagtcaaact ttgggcattc cagaagcgac tctttattct
     1261 tggcgtaaaa aagctagaat tcaagggaat actacacccg gaacgaaaaa aaatgtcgaa
     1321 aaatggtcaa gtgaagataa atttttaatt gtgatggaat cattctcaat gaaccaagta
     1381 gaacttgcgg aatattgccg aaagaagggc ttatttaaag aacaaattga ggcttggaag
     1441 aaaacgtgct tatctgcgaa tgaacaggaa gaaaatcgaa cgagagaact tgcaactgaa
     1501 ctaaaagaag agaaaaaaca agcgcgtcaa ttagagaaag aattacgtca taaggaaaaa
     1561 gcattagcgg aagccgctgc tctcttatta ctaagaaaaa aagcacacgc gatttggggg
     1621 gaccaagagg aagaatgatt tcatcttcag atcgcaaaca agctgttgaa cttattcaag
     1681 aagccaatcg caatggggcc cgtctcacct atgcctgtaa tgagctgaat attagcgttc
     1741 gaacctacga acgatggacg cgtgaaggta cgatagcgca tgatcaacgc cctttggcga
     1801 aacgtcctgt acccaaaaat aaactgacgg atcaagaacg cgaaaaaata atagaaaccg
     1861 tttcaaaaaa agaatttatg aatttgccac ctagccaaat tgtaccaaaa cttgcggatt
     1921 gctcgattta tattgcatca gagtctagct tctatcgtgt attgcgagaa aaaaatatgc
     1981 aacatcaccg tggccgaagt caagtttcac aaaaacgcat accgccaagt caccttgcga
     2041 cgaagccaaa tgaagtttgg acttgggata ttacttggct aaaaggtcca ataaaaggac
     2101 tcttttaccg gttatatctc atcattgatt tatttagccg taaaatggta ggctgggaga
     2161 tttgggaatc agaagaagct cgtcatgccg aaaaacttat taaacagacg gtgcttaagg
     2221 aacgtatcca aggacagccg ttggttttac actccgataa tggcagccct atgaaagccg
     2281 cgacttttca agtcttactc gaaaaattag gaattcaaag ttcttattca agaccacgag
     2341 tgagtaatga caatccttat tcggaagcgg ctttccgaac gttaaaatat cgaccagaat
     2401 atcccaataa cggctttaaa acaatagatg atgctcgaaa atggacgaaa gagtttgtcg
     2461 attggtataa cgattctcat ttgcatagtg ggattcaatt tgtgtctcct gcagcgtgtc
     2521 atgaaggacg acatctcgct attttagaga aacgaaagca ggtttacgag gacgctaaaa
     2581 agcgccatcc tgaacgctgg tcaaaaggtg ttcgaaattg gactccacat caatcggtgg
     2641 cgttgaatcc tttaaaagaa acagaaagtt aacaggagca taatctttca agattatcaa
     2701 ataaccctcg tcacccaata ggggaaaaag ttattcttcc cctattggat aacgaggcca
     2761 accgaccata gggaggttct gctttttgat aggtggaaaa taaaaatacg acaactatat
     2821 tgacaaacac
//
LOCUS       DACDTO010000160         2754 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDTO010000160 DACDTO010000000
VERSION     DACDTO010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.165"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(783..2123)
                     /gene="glnA"
                     /locus_tag="IWK70_002838"
     CDS             complement(783..2123)
                     /gene="glnA"
                     /locus_tag="IWK70_002838"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9615317.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(2151..2543)
                     /locus_tag="IWK70_002839"
     CDS             complement(2151..2543)
                     /locus_tag="IWK70_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9615318.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT          811 a          597 c          451 g          895 t
ORIGIN      
        1 tcctacttgc agcaacagtt tatatagctt atcgtagaaa caatcttttt ctttcaccct
       61 tatttacctc ctttacgtaa aaaacgtagt ccacagttct cggatttttg gcatatcttt
      121 tctattaaat aatatcaaaa acaaaacaat tccactaatt ttttcaaaga tgaccttttt
      181 ttcaacaaaa gtgccttgta gggattcatt gaaggtaacg aatcacataa gtaaaaaata
      241 aggagatgcg acaaaagtcg tgcctcctta tttctaaata aacggtgttc tcctgccaac
      301 tcctcggtca caagtcacaa tatccatcaa acatgagaag ctgtttttga aattatttct
      361 tgtgattcgg agttaaatgg taggctcaca acctctggat aaacggtatt caaaagccag
      421 tttctcggta ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt
      481 cttaatactc gaaactaaac gtctttttca aaacctcttg gtaaacggtg ttcaaaagct
      541 tgcatctgcg gtaataagtt cagccaattc aaaaacaaga aaagctgttt ttgcttggct
      601 attcttatta cttgttgcag ggcagctttt tcacaacctc tccactaaac ggtgttcaat
      661 cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat ttttccgtta
      721 gtttctctaa tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg
      781 ttttaataaa gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc
      841 gcccattcga gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca
      901 atcattacat tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc
      961 tggatatgtg cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct
     1021 gctggcggtg tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt
     1081 gccaagtatg ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg
     1141 cgagattctg gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca
     1201 ggcgcttcat aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct
     1261 gtgtaggcac gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt
     1321 tccaattcac cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg
     1381 caatgcatac cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt
     1441 ccatgcttgc gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca
     1501 tctaccacat ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt
     1561 gaagcttcca cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt
     1621 tcacctaaat ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg
     1681 atcccaccgt cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa
     1741 gaggtaaaac caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg
     1801 ccggcaaatg gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca
     1861 tgagcacttt cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg
     1921 ctttcttcaa ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct
     1981 aatactttgt cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct
     2041 gtgaacatca atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca
     2101 cctgttatct gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag
     2161 aattgtcttt gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc
     2221 aaaatctcat cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt
     2281 gcttcttgtt ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat
     2341 ccctctgaaa gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata
     2401 cgtcggtttc cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt
     2461 tgccgagcag acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat
     2521 cggcgcaatt ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg
     2581 taccaattaa aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta
     2641 ttttttataa aataacaaaa aaataagaac acaaaaaagg tatctagttg attttcgtac
     2701 cagatacctt ttatttttta ttatactgta ctatccttta ttagtgtttt tatt
//
LOCUS       DACDTO010000161         2564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDTO010000161 DACDTO010000000
VERSION     DACDTO010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.167"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            261..2414
                     /locus_tag="IWK70_002840"
     CDS             261..2414
                     /locus_tag="IWK70_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9615319.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          888 a          441 c          554 g          681 t
ORIGIN      
        1 ataacaaaac gtagttttcc acaaataaat gatccatcaa agtgtttcac gacttaagaa
       61 aacataattt tcaaaaataa gtttattttt taatgaaaaa aatagttgga tatgtaactt
      121 taagtgattg ctgtgaaaca aaaagttgat tttatcttga aattaggtaa agaatttctt
      181 gctccgccca ttttttcttt tttattaaga agtttttggg tacaatgaag catgaatgta
      241 aataggcgga aggataggaa atgatgaaag attttatgaa aaaaatatta aatacttctt
      301 ggttagaaaa aataagaaaa ggtgcacaag aacctaagaa caaaacgttt cgccggtccc
      361 atgttccttt tcgcttaaat tttctttttt tcattatatt tacgttattt gttgcgttga
      421 ttgctagatt gggttatttg cagatcgtga acggcgaaga gatggaggcg cgagtgaagt
      481 ctacttctac gattacggtc caagaaagta ctcctcgcgg aatgatctac gactccacag
      541 gaaaagcttt agttacgaat aaagcgaatc aggccatcac atatacgaga ggaacacaag
      601 ttacggctaa agatttgctt gaaatcgcaa cgaagctgaa ctcgctgatc gatgttccgg
      661 tagatgaaaa tctgacagat agagataaaa aagatttttg gctggctgat cccaaacatt
      721 tgaaagaagc cacaaaaaga ctttcggcaa aagaaaagca attagggaac agcgaacaat
      781 atagtgcgat ggtcaataaa gtcacagctg atgagattaa tttaaacgag gagcaattaa
      841 aaattgctac catctttaaa cggatgaata gtgcatatga attaaacact gtctatatca
      901 aaaactctgg tgttactgat caagagttag ctgttgtggc agaacgcgct tctgaacttc
      961 caggagtttc gactggcaca gactggacac gagaatataa tgcagcaagt tcactgaaaa
     1021 gtattcttgg cagtgtgacg actgaaaagc aaggacttcc agcagatgaa gcagagaaat
     1081 acctagcaaa aggttattct cggaatgacc gtgtaggaca aagttatttg gaaaaacaat
     1141 atgaagatgt cctgcaagga accaaaaccc aatatgaagt ttcccttgat aatgaaggaa
     1201 atgtttcgaa tcaaaaagaa atcttctctg gggaaaaagg ttctaattta atgttatcta
     1261 tgaatgcaga attccaatca aaagtagaag aaattttgaa aaggaattat caaacattga
     1321 tcaacaacgg caaagcacag tattcgcctg gtgcttatgc agtggctatg aatcctcaga
     1381 caggagaagt gctagcaatg actggtttca gtcatgaaca aggttccaaa gaaatcaccg
     1441 agaatgcttt aggcacgatt actagcgcct ttgatccagg gtctgtagtc aaggctggta
     1501 ctttgacagc aggctgggca agtaacgcga tcagcggaaa tcaagttttg attgatgagc
     1561 cgattcgtct gcaaggtgct tcagaaaaaa gctctgtctt taatcgaagc ggacaagttg
     1621 ctcttgatgc agtcaaagca tcggaattgt cttctaatac gtatatgatc aaagtggcgt
     1681 taaagatgtt aggactagac tatacacctg gaatgggact acctagttta gatgaagaag
     1741 ctaaagctta tcagcaattg agagactctt tcaaagagtt tggtcttggt acaacgactg
     1801 ggatcgattt gccgaatgaa tctcctggga tctcacgttc tgttgattat atgaagaagt
     1861 tcaatgcgga taatggcaaa gaatggtatg ctccaggtaa ctttaccgat ttggcatttg
     1921 gacagtttga cacatataca ccaatccaat tagctcaata tgcttcaaca gtcgctaatg
     1981 gcgggaaacg agtacagccg cacctagtca aagcgatcta tggcaatgac gaaaatggaa
     2041 acttgggtga agtgaagaaa gaaattggca caacagtaga aaatacggtc aatatttctg
     2101 cagaaaatat ggcgatcctc cgtgaaggat tccaccaagt tgtccatggt accgatcctt
     2161 acacaacagc caaaccatta gccagtgcga aaatggacct ttccgcaaaa acagggactg
     2221 cagaaacggt tgcggaaggt catccagata ttgcgacagt caatagtaat atcgtggcat
     2281 acggaccaac agacaatccg gaaattgcca tcagtgttgt cttgccgaac ttattagacg
     2341 aacaagacca cataaacttg acgattgcta aagaaataat ggacgcgtat tatgatatgt
     2401 tcatggcaaa ataaaaaaga ggtcgtgaaa aagccgctta gtttcgagta tgaagaaaaa
     2461 ttttgtgaaa atagcttctc acatttttgc ttaaatttga cttcataccg agaaactggc
     2521 ttttgaacac cgtttactcg gcaaaaaagg ttgtgacaaa aatt
//
LOCUS       DACDTO010000162         2564 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDTO010000162 DACDTO010000000
VERSION     DACDTO010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.168"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            84..1172
                     /locus_tag="IWK70_002841"
     CDS             84..1172
                     /locus_tag="IWK70_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615320.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            1169..>2564
                     /locus_tag="IWK70_002842"
     CDS             1169..>2564
                     /locus_tag="IWK70_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9615321.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISM"
BASE COUNT          937 a          284 c          445 g          898 t
ORIGIN      
        1 atagctaaag tatattttaa ttatgaattt ataattagtt gtaaaagtta atatttaacg
       61 gacaggaagt agggtgaaaa aatttgaaaa aaattttagt gttagcaaat acatattatc
      121 aaataatagt agcaatacaa atgaaaaata caattttttc cgatgagtat gttgaaatta
      181 ttatttctga tcattctaaa aacactaaag aaattgcgaa aaaaatagaa gaaaaaaaag
      241 tgtttgatgg ctgtcattat ttagaaagca aatcttttga tgataatatt gggtattttc
      301 aaaagattaa ggactgtctt cagatttctt gtggtaaatc aaatcgatat gcctattatt
      361 tgaaaaactg taaaagtgaa tattttgatg aatttatttg tttttcaatg ggagaggttt
      421 ggttggaagg aatatatgct tgcttatatc gaaataataa aaatgtacag gtatcaattt
      481 tagaagaagg tattttaagc tatggagttg acattattcc aacagcaaga acaaaagcta
      541 tcgcagtact caattactta aaaaaaagga ctccactata taacaaaagc tataattttt
      601 attgtttttt tcctaatata tatacaggaa gagagaatac aatacaaatt ccattagtta
      661 cagctgggtc aagaaccgca ttgcaacttt cggatatttt taaccctatg atagatattt
      721 ataaagaaaa aaaatatata ttttttacgt ctgtttatga ttttgagggc ggagaacctg
      781 tcggagaata tgaattagta tgcaaaatag cagacttagt aggaaaagat aatctaattg
      841 ttaagacgca tcctagagat gtaagaacaa tttataaaga taatggattt atcgaggata
      901 aaaattcatc aataccttgg gaggcgattc aattgtcagg cgattttaac gataaaatat
      961 ttatgacaat taattcagga agtgttattt caggtagtac aatgtctgca aaacctgtac
     1021 gcacgtatta tatgtataaa ttgtgtaaca tagaaggaaa tccgtcttgc aaaaagaatg
     1081 cgtatgatat tgagcgattg cttaagcaaa aaacgatgaa aaacatttta aagaatgtga
     1141 aaatagcaga aagcttagat gatatactat gagcaaaact aaatatttaa ttaaaaatat
     1201 gggaatatta acaataagta atttttcttc aaaaatttta gtttttttgt tagtcccttt
     1261 atatacaagt gtattatcca caacagaata tggcacatat gatttgacaa tttcaacagc
     1321 cacattattg tttccaattt taaccttaaa cattgtagat gctgtgatgc gctttttaat
     1381 ggataaagat aaaaataaaa aaaatatagc atctatagcg attaaagtta tatttttaag
     1441 cgtgatagtc tttggatccg taatgtattt gttaaatatt acaaaattat ggcaagatgt
     1501 ttatggatta gaaaaatata tttttttgta ttatatatcc tatgtatgga atcaatttct
     1561 aatacaattt gctaaagggt tagaaaaagt taaagctatg ggcatagcag gtattatcag
     1621 cacaattgta acaataattg ccaatatttt cttcttgctt attctgaaat ggggattaat
     1681 tggttttttc attgcaaata ttttatcaca gattatttca gctacttatc tgtcagtatt
     1741 agtgagaata tgggaatata tcgattttga ggctaaagat cctcagctaa agagagaaat
     1801 gctaaattac tcgattccat taattgcaac tgttgttgga tggtggatta atagtacagc
     1861 tgataggtat gtggtttcct ttatagtagg tgtctcggcg aatggcttat tgtctgtggc
     1921 atataaaata cctcaaataa ttaatacttt gcagggaata tttatacagg cctggcaaat
     1981 ttctgcaatt aaagaatatg gagataatga gactgcaaaa ttttatggaa atacatttgt
     2041 tatgattaat ttgttaatgt gcataacctg ttcaagtctt atcatattaa ctagaccgtt
     2101 ttcacatatt ctttatgcga aagactttta tgaagcgtgg aaatatgttc catttttatt
     2161 aatagctaat gttttaaatt gtgcatcagg tctgttagga cctatcttat cagcaaaaaa
     2221 agattctaaa tccatgatgt ggtctgcaat cataggggca ttagttaatg tgattatgag
     2281 tgtagtcctt gtttataaga taggggttca aggtgccaca atagccactg ttatatgttc
     2341 atatgtaata tatgttgttc gtaaacgagc tgtgggaaaa gatattttta taaagcatta
     2401 tcatattgtt gtggaatctt ggggattatt gtttgttcag gcaatcatag aaatatattt
     2461 taaaaattat gtaatagaaa ttataattat gattatttta attattataa atatcagtta
     2521 tattaagcag ttgtgttata gaggaagaaa cattatttca atga
//
LOCUS       DACDTO010000163         2501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.170, whole genome shotgun sequence.
ACCESSION   DACDTO010000163 DACDTO010000000
VERSION     DACDTO010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.170"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            144..494
                     /locus_tag="IWK70_002843"
     CDS             144..494
                     /locus_tag="IWK70_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9615322.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            908..1726
                     /locus_tag="IWK70_002844"
     CDS             908..1726
                     /locus_tag="IWK70_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9615323.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1799..2158)
                     /locus_tag="IWK70_002845"
     CDS             complement(1799..2158)
                     /locus_tag="IWK70_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9615324.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          823 a          361 c          461 g          856 t
ORIGIN      
        1 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
       61 tgggtataag tcatttatgt tcactctcct tgtatgcttt agcgggtatt acaatttgag
      121 tgtaacataa atggcttttt tatttgtctc gcttaattat acaaacggcg ttttataaag
      181 caatcattcc tgagaactta gatgaggtta aactttattt atgggaagat aacggagaaa
      241 ttgtcggttt tagtggtgta agtgcagaag aattagttat gctatttctt gatccagatt
      301 ttattggtaa gcgatatggt agtcaaatat taattgagct gattgaaact gaagatataa
      361 aaagaattga tgttaatagt caaaataaat atgcaaaaca tttttattta agccatggat
      421 ttgaaattga atctgaagaa gacttagatg gttttggtaa accataccct ataacgcatt
      481 tagtaaaaaa ataattcatg aatggtttat tacctgattg aacatgaata aaagtaatat
      541 ttttattttg atggcctatt tctaggttat gaattatcca gatcaaattt gtgtattaca
      601 ttcagtaata aggtgcttat cttaggatca acttatgata ctagctgacc aaggatgtac
      661 cagttataga aaatcagtta aagtaaaaat gataaaaatc ttatgtcaac actgatttag
      721 gctgacctgt gggttaggag aaagacaggt cggtagcttt taatacaaaa aatggcttgt
      781 atgtatctag atagttaact gaaattagaa atgttcttat ttttaacgga gtaagaaaaa
      841 taggctgctt taggtctgaa tactttttca atataatgaa gatatcaaag gcgacaggag
      901 tgtttttatg aataagaatc ctcataatct aaagttggta gcagtagata tggatggaac
      961 atttgctcgt actgattaca catacgatat cccaaggttc aaggctgttt tatcgcgaat
     1021 gaaagctgtt gattgtcaat ttgttgttgc aagtggaaat caatactacc aattgagatc
     1081 gttatttcca aattattatg atgaattaag ttttgttgct gaaaatggtg cttttgttaa
     1141 agataaaaca gaggtactat tttcagccga tatgcctaga aaagcgatcg aggtggtcct
     1201 tgatttatgt aaagactatc ctgaagtaaa aaatgtgatg tgtggtctga atagtgccta
     1261 ctgtgaaaga ggaaaggtga gtcaagcatt ttttgatcta accaatatct attaccatag
     1321 attagaatgg gtagctgatt ttgcagaagt tcaagataaa attttaaaat ttgctccgac
     1381 tatgcctgtt gaaaaaaccg aattttacgt tgaattgttc cgtgaaaaat tagccgggct
     1441 attagtacct accagtagtg gtcatggttc tgtagacttg attctttctg agtgccataa
     1501 aactttcggc ttgaaaatgt tagttgagcg cttggggatt aatcctgacc agtgcgttgc
     1561 ttttggagat gggggcaatg acattgagat gttggagtat tgtggtctga gttatgaaat
     1621 ggataatgca acggaagctg ttaagcaagt tgctaagcat caatgtcctt caaatgacga
     1681 agacggtgtt ttagtgacgc tcgatcgatt gttccctaac acgtaaacac taggtaattc
     1741 atcaaaaaac ctcgatataa gaaatattcg aggttttaaa actatattta gaacagattt
     1801 acgaagcgtc tgatgagtca aaatagactt ggacattgct atctaaaata ccactttcat
     1861 tctttaaacg aattgtttga tttgatggag ttacctctaa tactaaatgc ccattaataa
     1921 aggtagtatc gtattcagat gttttaactg ttgcttcttc gcaaggtatt gttaaatcat
     1981 atgctggaat ataatgttgt gtggaaacta ataaaaaatg atcaccaatc atacagcatc
     2041 cctctgctga agaaaattga ttagagccat catttaaagt taacaagaat ccatccttta
     2101 aacgaggtac ttgcttaaat acttctggtt gtacttctaa aaataaatta tcgctcattt
     2161 tcacgaccac catcgacttc cacagttgaa attgacattt tatttaacac acctattccc
     2221 ccttaactat aactcaattc tatataaaaa atattctatt ctcaaataat tgaacttttc
     2281 aaatcgccaa tttacttcgg ctttatcaga gctcattcat atcaagagcg attaacgctg
     2341 taaaaattaa accgaagatc tgataaattt tctctatttt ctacaatatt taaaacgctt
     2401 ccttagtcta ttatatatct atttctctta ggagtatgtt tagatgtttg aataatagtt
     2461 gcccaacttt aaaggttatt cttataatat tttacttcat t
//
LOCUS       DACDTO010000164         2486 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDTO010000164 DACDTO010000000
VERSION     DACDTO010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.171"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(243..464)
                     /locus_tag="IWK70_002846"
     CDS             complement(243..464)
                     /locus_tag="IWK70_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615325.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="IWK70_002847"
     CDS             complement(562..807)
                     /locus_tag="IWK70_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615326.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="IWK70_002848"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="IWK70_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..2408)
                     /locus_tag="IWK70_002849"
                     /pseudo
     CDS             complement(2031..2408)
                     /locus_tag="IWK70_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
BASE COUNT          850 a          429 c          348 g          859 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattggat gaatcggcta cactagacta gacagaaaaa ataaggtgtg tagaataata
     2461 aaaaacacac tggaggattt ttcatg
//
LOCUS       DACDTO010000165         2407 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDTO010000165 DACDTO010000000
VERSION     DACDTO010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.172"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWK70_002850"
     CDS             5..1150
                     /locus_tag="IWK70_002850"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9615327.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWK70_002851"
     CDS             1321..1779
                     /locus_tag="IWK70_002851"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9615328.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="IWK70_002852"
     CDS             1938..>2407
                     /locus_tag="IWK70_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9615329.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DACDTO010000166         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDTO010000166 DACDTO010000000
VERSION     DACDTO010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.173"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWK70_002853"
     CDS             207..2069
                     /locus_tag="IWK70_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9615330.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IWK70_002854"
     CDS             2069..>2400
                     /locus_tag="IWK70_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9615331.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDTO010000167         2384 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDTO010000167 DACDTO010000000
VERSION     DACDTO010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.174"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..642)
                     /locus_tag="IWK70_002855"
                     /pseudo
     CDS             complement(1..642)
                     /locus_tag="IWK70_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            851..1225
                     /locus_tag="IWK70_002856"
     CDS             851..1225
                     /locus_tag="IWK70_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9615332.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(1339..1968)
                     /locus_tag="IWK70_002857"
     CDS             complement(1339..1968)
                     /locus_tag="IWK70_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9615333.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(2102..2174)
                     /locus_tag="IWK70_002858"
     tRNA            complement(2102..2174)
                     /locus_tag="IWK70_002858"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2139..2141),aa:Thr,seq:ggt)
BASE COUNT          754 a          373 c          405 g          852 t
ORIGIN      
        1 ctaatgggtt gtctcttaag aatttacgat tcaatacgtt tgtcgttttg gcttcattgc
       61 aagtagaatg atgtacttta aaataagcaa cagtatagct cgattttaat cctctatttt
      121 tcatgattct actaattttt cgtcggctga tctgaatgcc tcgttttgat aaggcttttt
      181 ttatttttct tgagccgtag gcctttcggc tgcggataaa ttcttcagcg actacttctt
      241 caagttctga ttcgtctttc tttggttttg attgataata ataggactga cgtgatagac
      301 ctaatattct gcacatcgct gatatagggt aaagatgctt attcgcatcg attacttgtc
      361 tcttcgtccg aatatcagcg ctgcttgctt taaaatatca ttttccattt ctaattgctg
      421 gtttctttta cgtagttcta acaattcttt ttgttcaggc gtaagattat ctttttcttt
      481 gaatgaacca ctcgttttag attgctttac ccatttgtca aatgctgaag ccgttagttc
      541 atattctcga atgatttcta cacgtggctt tccagctaag taaagattga cgatttgttg
      601 cttgaattct tgtgaaaaag ttcttcgtgt tctcttagac ataaaaattc ctcctggtat
      661 gttttcttct agtctacaca ccttaatttt tctgtctagt taattgtagc ctatccaagt
      721 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
      781 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      841 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      901 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      961 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
     1021 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
     1081 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
     1141 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
     1201 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
     1261 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
     1321 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
     1381 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
     1441 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
     1501 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
     1561 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
     1621 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
     1681 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1741 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1801 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1861 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1921 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1981 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     2041 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     2101 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     2161 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     2221 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     2281 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     2341 gtattgcgtt atttagaaga aattgatatt atattcatgt actt
//
LOCUS       DACDTO010000168         2337 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDTO010000168 DACDTO010000000
VERSION     DACDTO010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.175"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..804
                     /locus_tag="IWK70_002859"
     CDS             <1..804
                     /locus_tag="IWK70_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9615334.1"
                     /translation="FFKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQG
                     WTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRS
                     IHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEK
                     KLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNG
                     LLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          854 a          311 c          466 g          706 t
ORIGIN      
        1 ttcttcaagc aaggaaaatc agccttagaa tattatcaac agtataagaa aaacaaatca
       61 aactgtggta gacgtccgct tgttttaccc gaggaacaat cagaatatat tcaaagaaag
      121 gttgttcaag gatggacacc cgatgtgatt gttggtcgtg cagcgtttcc tattagttgt
      181 tctgctcgta ccatttatcg tatgttcaaa aaggggctat ttgattcttc tgacttaccg
      241 atgaaaggca agcgtaaacc gaatggacat caagaaagac gtggaaaaca aactttccgc
      301 cgctctattc atgaacgtga aaaggattat agccaattct caaatgagtt tggtcacctt
      361 gaaggtgaca ctatcgtagg tctgaaacat aaaagtgctg taattacctt agttgaacga
      421 ttatcaaaag ttatcatcac attgaaaccg tgtggtagac aagcgattga tattgaaaaa
      481 aaattaaatc aatggtttga atctgtaccg aaaaacctat tcaaatccat cacttttgat
      541 tgtggaaagg aattttcaaa ttggaaacag atcagtaatg tcaatgatat tgccatttat
      601 ttcgctgatc caggaacgcc gtctcaaaga ggcctaaacg agaattctaa cggattgtta
      661 cgtagagatg gtttattgaa atctatggat ttcaattcag tagatgaatt ttttattcaa
      721 tctgtcgcat ctaaacgaaa taatattcct agaaaatcac tggattatcg aacgattatc
      781 gaacaccttt ggaagtattt ttgagttacg taagtattga tgatctgtct aatttaattt
      841 gacaattaag aaagtattaa cttgttttgt gaaaaagaga atttgaaaaa cgattggcga
      901 acaaatgctc ggtaggtatg gtataaatac agagctaatt ggtgtgatga aatcagatat
      961 tcgcgtgaga ttcagtaaag taggagttcg aaaacagcta ggtgacaaac agtattgctt
     1021 tggtacttcg gaatggttta tcaaattctt atcaacagtt cacttctctt tatgtttgag
     1081 tgatgattcg ttaggttatt gcttggaaag accggatgag agtagaagag tttaatttta
     1141 taaagtgaaa agaggtaatg tacgatatac gtagggctga accaatagag gataagcaaa
     1201 cgatgttaca ggcgcaaaat tcatagctta ctgttttgga gataacagaa gaggaaataa
     1261 tgcagaagta aggcgaaaaa aggacggaag cctaacagca tatgaaataa agaaaaatat
     1321 tataactact gataaatcgg tattgagcaa agtactaata ggagtcaaaa acattcataa
     1381 gctatgagtg tttcttggct acttttttat ttttatgtat ctacaaaagt ttccaggggc
     1441 agaacctgtg attattaatg gaagaacctc aagtattgtc ttaaatcatt gttatgaaaa
     1501 tggattaaaa gagttcatca aggtgtgatt aacaagttac ttttcattac atcgaatttt
     1561 tcagaagttg cttatattgg tgatgatatt aatgatttat catgtatgac atctgtgaag
     1621 aagcgggagg aatagtagaa tgtccaaaaa atgccacaag aaatgagatt gaattatctg
     1681 attttattgt aactcacagt ggtggtgacg aatctgtgat agagtttatt gagtggatat
     1741 tggaatcgat atagtgtagt agtacagaaa aattatcata caattaatac taggcataag
     1801 aatattggca tattctagag ttaaaagtaa ctattcgaaa aggataaggt tagaattttt
     1861 tgttttgcca gtggaagaat ataaaaaggg cattggaaat taatatatta gaatgctaag
     1921 cggagaaaaa aatatatgaa aagtacgtcc ttgtgatggg atggaaaaat acacttattt
     1981 caaacaacga taatataatt atgtgaaccg aagtatactc gcttctaatt caatcctcaa
     2041 atcccgacag tcgcgacttc aaaaaagcgt atagttttaa tgtatcccca tatgatccga
     2101 aaaatggtaa tttacaatta acagtttgat gatgttgaag atgtggaaat gctgaaatta
     2161 ccatgatgag aaacacatta attaaaggga attaattaac tagaatataa tctgattcaa
     2221 tttgaattaa gcaaaaaaag gctgagtttt aaataatcgc gaattgtaaa attaagttag
     2281 aaaaataaaa aggcatttat ggtacactca aattgtattt ccgacgaaag aaaacaa
//
LOCUS       DACDTO010000169         2254 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDTO010000169 DACDTO010000000
VERSION     DACDTO010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.176"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWK70_002860"
     CDS             169..1539
                     /locus_tag="IWK70_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9615335.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWK70_002861"
     CDS             1560..1844
                     /locus_tag="IWK70_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615336.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
     gene            complement(2120..>2254)
                     /locus_tag="IWK70_002862"
     CDS             complement(2120..>2254)
                     /locus_tag="IWK70_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9615337.1"
                     /translation="FNQVNQGFISSVASKRNHIPRKSLNYQTPLEVFLSYVNGKFCLA
                     "
BASE COUNT          776 a          327 c          474 g          677 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 catattatta tttgtcaaat taagcgagac aaaactttcc atttacgtaa ctcaaaaaaa
     2161 cttctaatgg tgtttggtaa tttagtgatt ttctagggat atggtttctt ttggacgcaa
     2221 cggatgagat gaatccttga ttgacttggt tgaa
//
LOCUS       DACDTO010000170         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.177, whole genome shotgun sequence.
ACCESSION   DACDTO010000170 DACDTO010000000
VERSION     DACDTO010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.177"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWK70_002863"
     CDS             <1..378
                     /locus_tag="IWK70_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9615338.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWK70_002864"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWK70_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9615339.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..2026
                     /locus_tag="IWK70_002865"
     CDS             1859..2026
                     /locus_tag="IWK70_002865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615340.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAFTGSVAKF"
     gene            2080..>2232
                     /locus_tag="IWK70_002866"
     CDS             2080..>2232
                     /locus_tag="IWK70_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9615341.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVREILYDRGINVSH
                     TTIYRWV"
BASE COUNT          628 a          371 c          483 g          750 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt ttactggttc tgttgcaaag ttttaaatct actatcaaat
     2041 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     2101 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     2161 tatcgtgaag ttcgagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     2221 tatcgttggg tg
//
LOCUS       DACDTO010000171         2232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDTO010000171 DACDTO010000000
VERSION     DACDTO010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.178"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IWK70_002867"
     CDS             <1..544
                     /locus_tag="IWK70_002867"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9615342.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="IWK70_002868"
     CDS             complement(900..1472)
                     /locus_tag="IWK70_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9615343.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="IWK70_002869"
     CDS             complement(1488..2093)
                     /locus_tag="IWK70_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9615344.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          454 c          338 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ct
//
LOCUS       DACDTO010000172         2189 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDTO010000172 DACDTO010000000
VERSION     DACDTO010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.179"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..2127
                     /gene="acm"
                     /locus_tag="IWK70_002870"
     CDS             <1..2127
                     /gene="acm"
                     /locus_tag="IWK70_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9615345.1"
                     /translation="IITNITSLIPVHVYADAGRDISSNVTSLTVDPTNITDGGNIKVK
                     FSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVGTLTVRKDSAQVVF
                     NENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATVNVTKPASGSSSSV
                     FYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRLDPSTFEINQIHLG
                     EQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRKIMVSYKTIIENPE
                     QINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGELKIFKYINDTEIG
                     IPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVKEKEAPNWIDFDPL
                     SSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPSIYFKLFRASTNNW
                     EPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGNDYVPSGYQKIENG
                     LVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQHKEVSEKDDWRYR
                     FTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNISIPGSHITPWKNF
                     PEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITNKSKSILPKTSSKK
                     SSFAVIIGLLIVTICAGIFLQKYKKV"
BASE COUNT          863 a          351 c          419 g          556 t
ORIGIN      
        1 attattacta atataacatc actgattcct gttcatgtct atgcggatgc aggcagagat
       61 atcagcagta atgtcacttc gttgaccgtg gacccaacga atattacaga tggaggaaac
      121 ataaaagtaa aattctcgtt tgacgaaaaa aaacaaaata tccaaccagg tgattatctt
      181 tggatcaatt ggccaagtga aggaaatatt cgcggtgaag gattccaaaa agaaatccca
      241 ttaatgattg aaaataaaaa tgttggaact ttaacggtca gaaaagattc tgcgcaggtt
      301 gtgtttaatg aaaatattaa aaacctagac tcagtagaag gttgggggca atttgagata
      361 caggccagaa acgtaaccga taccggtgaa gaaaatacgg gaccattcac cgtgacgagc
      421 ggtgataaaa cagctactgt aaatgtgaca aagccagctt ctggttcttc ttctagcgtt
      481 ttttattata aaacaggaga catgctacct gaagatacta agcacattcg atggtttttg
      541 aatattaaca ataacggcac ttatgttgaa caaccagtta aaatcagtga tgaaatccaa
      601 agtggacaaa gattagatcc aagtacgttt gaaattaatc aaatacactt aggtgaacag
      661 aaggtttata gaggagaaga gggaatccaa caatttttac aagatttccc cagcgctaca
      721 tttaattttt cagtaactga taattacatt gaaataacta taccaaaaaa ctttgtcaat
      781 cttaggaaaa taatggtgag ttataagacg atcatagaaa acccagaaca aataaatttt
      841 gagaatcatt cggaagcttg gtttaaagaa tttaataaac ctgctgttga tggagaaagt
      901 ttcaatcata cagttaaaaa tatttcagct tctggtggag tcaacggtac agttagaggc
      961 gaattgaaga ttttcaaata tatcaatgac acagagattg gtatcccaaa tgtgactttc
     1021 gaattaagac gagcagatga acagcctatt caaggacagt ctagtatcct gttgacttcg
     1081 aatgaacagg gagaagctag tatcaaaggc ctgcaagtag gagactatgt tgtaaaagaa
     1141 aaagaagcac ccaattggat tgattttgat ccactaagtt caaatgaact caaattctcc
     1201 ataaacgaaa atgatacaga gggagtcagt ttacctattt ataacaagaa aaaggttacg
     1261 aacatcacag caactaaaat ttggaacggt ggaaccactc caagaccgag tatttatttt
     1321 aaattattca gagcaagtac aaacaattgg gaaccagttc ctgatgcaga aacaaaacga
     1381 ttagacaatg gaataacttc agtgacttgg gaagatatcc aacagtatga tgatagtgga
     1441 aacgaatata cgtttaaagt gcaagaagta gatcaaaacg gaaatgatta tgtcccttca
     1501 ggttatcaaa aaattgaaaa cgggctagtc gttacaaatg agaataaaga agtcatttca
     1561 gtctcaggac aaaaaacatg ggaagacaat gagaaccaag acggcaaacg accgacgacg
     1621 atcacagtaa atctattagc cgacggtcaa ccgatccaac acaaagaagt aagtgaaaaa
     1681 gatgattggc gctatcgttt cacaaatctg ccaaaataca gagacgggca agaaatcatc
     1741 tatactgtaa cggaagacaa cgtgcccgaa tacagtacga cgattgacgg gtataacatc
     1801 aaaaacagtt atacaccgga aaaaacaaat atatctatac caggtagcca tataacaccg
     1861 tggaaaaatt ttccagagaa gacagagaga gagaaaatta gaaatctatt ctctcaaaaa
     1921 ttgcaaatat ttgctcatac aggagaatcc agcgaacaaa gaagcaatta tattagtaag
     1981 agagctgtac ctaaacagat aacaaataaa tcaaaaagca ttcttcctaa aacaagtagt
     2041 aaaaaatcta gttttgctgt aatcattggt ttattgatag taactatctg tgctgggata
     2101 ttcttacaaa aatataaaaa agtttagtta gaatataata ttttttgtta aatatgactg
     2161 aaatcaaaag aataaaattc cgatttata
//
LOCUS       DACDTO010000173         2102 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDTO010000173 DACDTO010000000
VERSION     DACDTO010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.180"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            127..645
                     /locus_tag="IWK70_002871"
     CDS             127..645
                     /locus_tag="IWK70_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615346.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            653..1693
                     /locus_tag="IWK70_002872"
     CDS             653..1693
                     /locus_tag="IWK70_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9615347.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            1942..>2102
                     /locus_tag="IWK70_002873"
     CDS             1942..>2102
                     /locus_tag="IWK70_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9615348.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          740 a          253 c          428 g          681 t
ORIGIN      
        1 caatctggaa aaacttcagt tgcaaaaaat aagtatgtta ttgtttatag gtgtatgctg
       61 ttatcgtatg ttatttaaaa catctttgtt tggggattgt gctgttttaa ttgcactata
      121 tgtcatatgt tatacactga aaaaaacgat aataatattt ttgagagaca cagaatcata
      181 ggaacaattg tatgtataac agttatacca cttctcatat ccataggaat acttttcaaa
      241 tttataccaa atgcaatggt taaccagatg gttcatattg gttctccatt gatggttata
      301 gcaagtatat ttttatttta tgtatttaaa aatttttcat tttacagtaa gtatataaat
      361 aaacttgcga gaaccagttt aggggtatat attttacatg aaaatcatta tttcattgat
      421 ggtatgaagt ctagaggctt aatatgggat gggatatttg gattaggcaa aaggttttta
      481 gatagggatt tttggatttg gtttataatt gcagttgtag ttgtgtgcat aatttgcatg
      541 gctatcgatg ttgtaagaga aaaatttgaa agacagattt ttaaaattaa gagtgtaaag
      601 aacctgtgta aacttatgaa tcatgttata aatatatacc cataattaat aaatgaggag
      661 aaaagaagtg aaagttatat cattcggaga attattattg agacttgcag cacccggtta
      721 ttcaaagtta tttcaaagag atagtttgga gactacattc tgcggagggg aagctaatgt
      781 agcggtatcc ttagcaatat ttggatatca atcagaattt ataacaaagc ttccggattc
      841 agatgttgga tatgcggcag ctaatgcttt gcgttatttt ggagtaaatg ttgataatat
      901 tatatttgga gagggacgca tgggactgta ttatttagaa aaaggagcag ctcagagacc
      961 atctaaagta atttatgaca gaaaattttc tgcaatttcg ttagctgata gtgatgaatt
     1021 taagtggaag gagatcctaa acgatgcaaa ttggtttcat tggacaggga taaatccagc
     1081 attgtctgag aatctctttg agacgtcctt agatgcatgt caggctgcca aaaaaagagg
     1141 tataacaatt tcttgtgatt taaattatcg atccaagctt tggacatcag aggaagcaaa
     1201 agaaaagatg gaacagatac taccattggt tgatatttgt atttgtaatg aagaagatgc
     1261 agaaaaagtg ctaggaatta aagctgagca tacaaatatt aatagtggag aattaaataa
     1321 agatggctat gtgcaggtgg ctagggagat tcacaagaag tttggatgta ggtatgtagc
     1381 tacaactctg cgtagaagtt attcggcaag tagaaatggt tggagtgcaa tgttatacga
     1441 agcagaaaaa gataaagcgt atttttccac agaatacaac atacaaattg ttgatcgcgt
     1501 tggtggtgga gacagctttt ctgctggtat aatatatgcg ttatgtgaaa atatgtccgg
     1561 acaaaaggct attgaatttg cgactgcatc tagttgttta aaacatacta tagaaggtga
     1621 ttttaatcga actacaattg aggatgtata taatttaatg aatagcggtg gaaatggtcg
     1681 cgtgttgcgt tagtcaacat ataaagataa atcacagtaa tttttatggt gattatctgt
     1741 cttagtagtg attagaataa cgtgaaaata aattgttgag cgtttatact gttttgaaaa
     1801 ataaatttaa catattgaaa aatacaaata tatacgtata ttataaagcg ccgattgtaa
     1861 aattaagcta gacaactaaa acagtcattt gtgctacact caaaatagtt ccgaccaaag
     1921 aattataagg agtgagtgca aataacctat acctatctta caacggatga acttgtaata
     1981 atagagtctt atttgaaaat taaccaatct gttgctaaaa ctgcgcattg cttgaatcgt
     2041 tcaagtcaaa cgatccataa agtatatctg ttcttcaagc aaagaaaatc agccttagaa
     2101 ta
//
LOCUS       DACDTO010000174         2025 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDTO010000174 DACDTO010000000
VERSION     DACDTO010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.181"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..425)
                     /locus_tag="IWK70_002874"
     CDS             complement(<1..425)
                     /locus_tag="IWK70_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9615349.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="IWK70_002875"
     CDS             complement(534..1565)
                     /locus_tag="IWK70_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9615350.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          626 a          330 c          348 g          721 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggtagga gcaattatat tgagaattaa tcctttttta tataa
//
LOCUS       DACDTO010000175         1985 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDTO010000175 DACDTO010000000
VERSION     DACDTO010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.182"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..223)
                     /locus_tag="IWK70_002876"
     CDS             complement(<1..223)
                     /locus_tag="IWK70_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615351.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLA"
     gene            461..1075
                     /locus_tag="IWK70_002877"
     CDS             461..1075
                     /locus_tag="IWK70_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9615352.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1099..1449)
                     /locus_tag="IWK70_002878"
     CDS             complement(1099..1449)
                     /locus_tag="IWK70_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9615353.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1443..1706)
                     /locus_tag="IWK70_002879"
     CDS             complement(1443..1706)
                     /locus_tag="IWK70_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9615354.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          687 a          334 c          302 g          662 t
ORIGIN      
        1 gagcaagtaa tggagaaact attaaataat tttcagtaac agttaagtcg ttagttgggt
       61 taccaatggc tattccgtcc ctatctaaag gagttaattc ttggttatta tctactacag
      121 tagttggttc atttgtacta tcagcaaatg taggaattgt agttccagtt acactaattc
      181 caatagttaa aatacctaaa gataaaataa acttattttt cattgtctat cacctcgaaa
      241 tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga taaatcaagt tatatactag
      301 aaaattcata aaatatattt ttaatgtcat aatcgtttaa attttcttga ttgacattta
      361 tttacaaaaa atattgccat ttaaaaagaa aagtgacata tggcttcctc gtgtatgatt
      421 agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt ttgagagttg catacatacg
      481 tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa gaaatgaaaa aattcggtgc
      541 agagagaatt ttcatcgaga aacaatctgg cgcaacgatt actcatcgac ctgttttcca
      601 agaagcatta gattacgtcc gagatcaaga tatttttatt gtggaagcta ttgatcgact
      661 tggaagaaat tatgacgaga tcatcgcttc agtcaattac ttgaagaaga aaaatgtgaa
      721 gctgatcatt actagtttac cgatcatggc tgaagcaatt ggcaatcctc tcctggataa
      781 attcattaag gatctgatca tccaaatttt ggccatgatc gctgaacaag aacggactga
      841 atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa gcaaacggcg tctacaaagg
      901 gcggcccaaa ttatacagtg cagacgcaaa agatccccaa cgtcgccttg tttacaaaag
      961 catcgttgaa gatcttaaaa atggtgttgc gattgctaag attgcaaaag actacaatgt
     1021 tacgagacaa accgtatata gaataaaaaa agacagcatg gtcaatcacg aatagttgat
     1081 catgctgctg tttctatttt atttgcttac tttttgaaaa actgttctaa ctttcatcaa
     1141 taactcctca actaaactat catgcgcttt ttccaaaaaa atacactctc tggcttcata
     1201 atcaattgtc gttagttgat ctaataatac ttttcctgtt gtcttcattt catattctga
     1261 gtttatattt acatatagcg gatagtttct agttgtacta gaaattggag caactgtaac
     1321 aacgttacta taatccgcaa taatatcatg acttaaaaca atataaggtc ttttacctgt
     1381 ctgttcgtgt ccagatcgag gtgctgtatt caataataga atatcgcctt gtctaggttt
     1441 tctcaccatt tttcattacc tactgcttca cctaaatttt gaatcgtcac atgatttctt
     1501 tcatcagtgt aattttcaaa taactgttca attgttaatt cattttctat attagccgct
     1561 tcgataatta gccgcttctg accgtttact tcttcttgta ttatcttcac tttgtcttct
     1621 gtatgaattc caagatattc cataatatac tttggcaatc taaggccatt actatttccc
     1681 cattttttta taggtaacat ttccatagta gacacctctt tctgtatata catagtataa
     1741 catacataag aaaaaacgta tatacttaat tatccttttc tattaaatta gattaacatt
     1801 ccatgaatcc ataaactatg ttaacattta tatgaaaata gtcgaaagga aatcataata
     1861 tgtttaagaa aacaaaatct ttagaatcag gtaagaaaaa aattgatcta aaaataatct
     1921 cttctggata ctttaaagat ttcatagtta ttgatacaaa atggaataaa aaggacatag
     1981 aaaat
//
LOCUS       DACDTO010000176         1982 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDTO010000176 DACDTO010000000
VERSION     DACDTO010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.183"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWK70_002880"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWK70_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9615355.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWK70_002881"
     CDS             852..1040
                     /locus_tag="IWK70_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9615356.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWK70_002882"
     CDS             1053..1610
                     /locus_tag="IWK70_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9615357.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWK70_002883"
     CDS             1642..1884
                     /locus_tag="IWK70_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9615358.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          723 a          305 c          406 g          548 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 tt
//
LOCUS       DACDTO010000177         1786 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDTO010000177 DACDTO010000000
VERSION     DACDTO010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.184"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWK70_002884"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWK70_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9615359.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          499 a          307 c          352 g          628 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagt
//
LOCUS       DACDTO010000178         1726 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDTO010000178 DACDTO010000000
VERSION     DACDTO010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.185"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..510
                     /locus_tag="IWK70_002885"
     CDS             <1..510
                     /locus_tag="IWK70_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HAP9615360.1"
                     /translation="NTTFEIEFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSE
                     QYLERNLAMLKLANHVGKGWFAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKK
                     ILLHTIDLYEETTELSALSASLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYT
                     KILYAEVSK"
     gene            507..>1726
                     /locus_tag="IWK70_002886"
     CDS             507..>1726
                     /locus_tag="IWK70_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9615361.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIAN"
BASE COUNT          667 a          222 c          291 g          546 t
ORIGIN      
        1 aatacaacat ttgaaattga gtttttagat gtgaataaag aaaatattct aacttatgtc
       61 agtccaatta tagatgacgc attcaaagat aaaaaaacaa taactgctta taaagaagaa
      121 atacaaagtg aacaatattt agaaagaaat ttagcgatgt taaaactagc caatcatgtt
      181 ggtaaggggt ggtttgcaat tgagttaggt gaaaaaattg actttttaat caaaattcct
      241 aagtatattc ttaatgctat tgcttttgca tgtcaggaaa tagtagatct ctcaatttat
      301 aaaaaaattc tactccatac aatcgacttg tacgaggaaa ctacagaatt gtcagcttta
      361 tcagctagtt taaaaacaag tgatacagct gatttgaata tagcaataat ttctttatta
      421 gagtactttg gtaaagatga ggatgttgtg tatctaatca atcagataga aaattacaca
      481 aaaatacttt atgctgaggt ttcaaaatga caaatatctt cgaaaaaatc gtaaatgatt
      541 taaatttaga acaaaaagat gttgtggaaa gtgaagataa cctttatgta aatgcatgtc
      601 ctgggagtgg gaaaacaagg gtacttacaa ggaaaattgc ttaccaagca attaaacacg
      661 atggatcact aaaaagaata attgctatca catacactaa tagagctgct gaagaaatta
      721 aggaacgcct aagtttactt agtatagatg atgatattaa tatttgggtg gggacaatac
      781 atcagttttg tcttgaattt attatttatc cttttaaaat gaatctctca agagtatcaa
      841 aaggatttac aattattgat gattatactc aaagagctta cgtcagccaa attaataata
      901 tgctgaattt aaatctcaaa acgtatgaac tgaacaaaat tgatttgtca ctttctcatg
      961 attttaaaat taatgaagtt cagtatactc aagtttctga aatgtatcac actatgttgg
     1021 aaaaaaataa agaaattgat tttgatatga ttttactagt agcgtataaa attttaactg
     1081 taaacagtaa cgttgcacgt aatattgcta gtaatataag ggcgatttat gtggatgaat
     1141 ttcaagatac tagagaatta caatacaatg taatagctaa attacttcaa aacaaccctc
     1201 aaattcagtc aatgtttgtt ggagatattg accaagcaat ttatggatca ttagatggta
     1261 tagctaaatc agttgaagaa ctagaaatgc taactagtca cacttttcaa tcaaagacgc
     1321 tacatggctg ttacaggtct aatcagagat tagtagactt ctactctaat tttcagagtt
     1381 gtccttacga gattgaaagc agagggaata ataaaaacga ccgtggtttt ataagttatg
     1441 attgcaaagt tactaaggag gatttaccca acataataaa aaatataata aaggaaaaga
     1501 ttgaaagcgg cattcatgaa aaagatattt gtgtacttgc tcctcaacac tatcctctgt
     1561 tttcactggg tgagtattta aagaaagaat taccgtattg caattttgat gctataaata
     1621 tatcaccaat taaggtaaat aatcatagtt tatttttcaa gttagcaatt ttgtacttta
     1681 ctgaatcagg tgaaaaggtt cgaagaagaa agctaattgc aaacga
//
LOCUS       DACDTO010000179         1680 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDTO010000179 DACDTO010000000
VERSION     DACDTO010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.186"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..251
                     /locus_tag="IWK70_002887"
     CDS             <1..251
                     /locus_tag="IWK70_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615362.1"
                     /translation="FKGFDFNSSTEFKLALYAQFFIEKLNRFQEIVEDSDFNTFNSHK
                     PMRGTYIKFLENYNDLKSTNNYQFGNLSYEMISKVLSL"
     gene            258..770
                     /locus_tag="IWK70_002888"
     CDS             258..770
                     /locus_tag="IWK70_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615363.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
     gene            1041..1664
                     /locus_tag="IWK70_002889"
     CDS             1041..1664
                     /locus_tag="IWK70_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9615364.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          657 a          203 c          280 g          540 t
ORIGIN      
        1 aatttaaagg ttttgacttt aatagctcta ccgaatttaa gttagcactt tatgcacaat
       61 tttttattga aaaattaaat aggtttcaag aaatagttga agatagtgat tttaacactt
      121 ttaatagcca taaaccgatg agaggtactt atataaaatt tctcgaaaat tataatgatt
      181 taaaatctac caataattat caatttggaa acttgtctta tgagatgata agtaaagttc
      241 taagtttgtg atgataaatg aaattaaata gtataaatga tttatttgct attataggtg
      301 ttattgctaa catacttttt tcgtgtttga attttagatt tactcaaaag aacaaaaaga
      361 acaaactcag tatagaacaa atgaaaaaag acattgaaac tgatttaaaa aatgaccata
      421 aagaaagaag aaaaattgaa gaatatactt ctttaatgga aaagtgtaaa gcaactgatg
      481 caatttttag ctttttaaga aaaagtgatg agataatcac acgtgaaaat atagatttag
      541 aaaatgagat tagtaaactt gaatctatct tttccgatat aaattctctt aagtatttaa
      601 tcgtcgaaga aaattcggat agctttttag aatttgaaaa atatctaaca gatcttatag
      661 ataatagaaa taatgaatta atgagacaaa acattgcaag attccttact aaattagttt
      721 ctgaatggaa aagtattatt tctcaaaata tggataaatt aagatattaa ggatgttagt
      781 tttaaaataa actaatgtcc ttatttttgt gttcacattt ctgttaccta agaaatactt
      841 aaaggaggtg atgtagccag caacgactac aacttgaacc cgctatctac agtttatttg
      901 ttgtttcttt tgggtatact ctaatggtac aaatccatat ttgcttaaag cgttgatttt
      961 tcagcggttt ttctttattg caaagtgtac cctaagacct gtatactaat agtaaataga
     1021 ttggatagga atgatggcaa atgtatttag cttatttaag agtttcttcc gacgatcaaa
     1081 gtttagctcg gcaacataag ctaattgaaa attggtcagt caaaaataat attgctgaag
     1141 acgatttaaa aattttcgag gaaaaagtct ctggaaaaaa tattgaaaac agaccagagc
     1201 tgcaagagct tatttctttc atacgtgaac gagatacgat tattattcct agtcttgatc
     1261 gattagcaag aaatagtaaa gatacaaaag acttacttca attgtttcgt agtaaaggtg
     1321 cttctattga aatattagac ctaccgtcgt ttaatggggt aactgaccct gcattaagag
     1381 acttgctaac aaatttagtc atagaagtgt ttagctatgt tgctgaaaac gaacggaaaa
     1441 aaatcaaaga gcgtcaaaaa caaggcattg aaattgcaaa gaaaaatggt cgttatactg
     1501 gtaaaaagat tcaataccat gccaatgcaa caggaaaaaa caaattgatt tacgatgaaa
     1561 ttgttgatgg tctgaagaaa aaagaaacta tttctaaaat agcaaagaac gcaggagtta
     1621 caagaaaaac cgtttatcga gtaaaaagag atttaggaat gtaatatgtt ccagttagaa
//
LOCUS       DACDTO010000180         1677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDTO010000180 DACDTO010000000
VERSION     DACDTO010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.187"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IWK70_002890"
     CDS             <1..354
                     /locus_tag="IWK70_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9615365.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..903
                     /locus_tag="IWK70_002891"
     CDS             355..903
                     /locus_tag="IWK70_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615366.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1163..1533
                     /locus_tag="IWK70_002892"
                     /pseudo
     CDS             1163..1533
                     /locus_tag="IWK70_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1533..>1677
                     /locus_tag="IWK70_002893"
     CDS             1533..>1677
                     /locus_tag="IWK70_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9615367.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIY"
BASE COUNT          652 a          211 c          354 g          460 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca
      541 aaaaaagttg gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta
      601 aaactgtcga ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat
      661 atgacaagag atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg
      721 caagaagata agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa
      781 aaatatccaa ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag
      841 ataaaacaag ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga
      901 taataatcaa agaagaacta agtaactacc tttaaacggt agttacttag ttcttctttg
      961 atttgcatga aatagagggg atttattttt ctggttacgt gattaaaaaa tccaaaaaat
     1021 gagcatagga acggttatga ttttagggtg tgttttaatg agatattgtg atttcattaa
     1081 caagtgtatg ataatctgtt gttgagttag taaataagta atagagctaa ctctaaaata
     1141 aaactattag gaggagatgt aggtgaaaaa gtttaaaaag aaaatatatt taggattggt
     1201 tgttggggtg agtatgtttg ggttcatggc tcatactaaa caagtaactg cagatactaa
     1261 ccaaaaagaa aggattatga gccaattaga tgactctcaa ataagtatgt acccaatgag
     1321 ctaattgtca gtttaaaggc tacacaaaga atttcaacag agaaaaataa accgcaaaat
     1381 ttattgggtg taaaagttat ggtggaaaaa gatttgactc ttaggacaaa tgaaagcgat
     1441 tgtaataagc gagatcaagg gttctgttgc aaagttttcc aaaaaatcta ttttagtgta
     1501 aaattgagaa aaaagacaga gaggacagag taatgaatca ttttaaaggc aaacaattca
     1561 aaaaagacgt cattattgtc gctgttggtt actacctgcg ttacaatcta agctatcgtg
     1621 aagttcagga attgttatat gatcgtggaa taaatgtttg tcatactacg atttatc
//
LOCUS       DACDTO010000181         1674 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDTO010000181 DACDTO010000000
VERSION     DACDTO010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.188"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            594..>1674
                     /locus_tag="IWK70_002894"
     CDS             594..>1674
                     /locus_tag="IWK70_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615368.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFG"
BASE COUNT          577 a          162 c          288 g          647 t
ORIGIN      
        1 aattaacaag ttacaaaaaa atattataaa gaataatatt ttctaaaaag taaactatag
       61 taatgtggat tatcaccttc caacagtata taaaatgagc gtaaaaacgg ctatttttgc
      121 cttaacaacg atttttttgt atacaaatac gatatgctca ttaattattg gaatttgtag
      181 tgctgtttaa attaatgaag gtgtaattat atgagtgatc attttgaaaa agtttgttga
      241 gaagatgtgc ttctagtcat gtgatgggag aaaataattt aactgttaga gtgaatagtt
      301 aaattattct ggttatatga aaaaagtagt ttctaaaata atcttgcagg gattaactta
      361 aagaaattga gagaatagtt tctttgaaat ttctatatac aatgagaagc tgaaatttac
      421 ttttatttga tgactggaaa tagaaattaa atatattgta ttgttacatt aaagaaaact
      481 tgtatggaaa aatcagtaca ataccattat ttaatgttct tcacttaaag cgcaaaaaat
      541 atcagtttat taaactcgta tatagaacaa atatatgtag acggggcgaa acgatgagtg
      601 taaaaaaaat gctatttatg gtaagtacaa taattgtatt aacatcccga ttttggggaa
      661 ttaatactag tttatctatg cgatatttta ttatggcaat atgggattta tattttatgt
      721 taagctattt ttcttatcgt gagaaaacat ataaaaaata cgttatcaag aataactttt
      781 ggttgactat aatgccgttt gtgatatttt ctatctatac tctaataata tggggaattg
      841 gttcaaatgc agaatttgaa aattttataa agttatgttc cacgttgctt tatttaattc
      901 ttgcatatgg ttacgcatgt acagcatttt acttatttaa ggcagaagga attgatttta
      961 ttttttggtc aggtgttatt tcatatatga gtggttcagt tttttattta attgtatctt
     1021 atggtttaaa tggtttaatt tcttatataa aaagtcttat aacaggtgaa acaaatactg
     1081 caaattttgc tatggaggta catgatttaa cttttgcgat gggggttttt ttcctttatt
     1141 acgtattttt tgaaaataaa aatacaaaaa aacataagat aaaaattatc gtatctttaa
     1201 ttttaatttt catgggattg aagagaattg aactgttagc tttaagtggt gcaattctag
     1261 tgtattatgt tttattaaag tggggaaaaa agattcaaat ccgtgcatta gtgtgtacta
     1321 tttgtgcaac tgttatttcg ttagtgttta tttacattat agatacagga attttggcat
     1381 tgcttatgtc aaatttgggc attacagatg acggtgcgag gctttcattt tatctatatt
     1441 catctaaatt ttatgaactt ggtttaggat atgtaggaac aggatggaca tggttttcaa
     1501 aatacttttt taatctttat ttatcgcact ttaggttaaa tggtggaaga attgcagcaa
     1561 gcctacacag tgatatatta actatttata tagaagttgg atgtatagtt tttattatat
     1621 ggatgttttg gttatttttt gtgaaaccga taagactaac taaaaaattt ggat
//
LOCUS       DACDTO010000182         1658 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDTO010000182 DACDTO010000000
VERSION     DACDTO010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.189"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWK70_002895"
     CDS             949..1422
                     /locus_tag="IWK70_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9615369.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWK70_002896"
     CDS             1431..1658
                     /locus_tag="IWK70_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9615370.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
BASE COUNT          548 a          265 c          246 g          599 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataa
//
LOCUS       DACDTO010000183         1634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.190, whole genome shotgun sequence.
ACCESSION   DACDTO010000183 DACDTO010000000
VERSION     DACDTO010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.190"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            101..868
                     /locus_tag="IWK70_002897"
     CDS             101..868
                     /locus_tag="IWK70_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9615371.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="IWK70_002898"
     CDS             1060..1458
                     /locus_tag="IWK70_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP9615372.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          592 a          212 c          315 g          515 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtattg
     1621 cctttttatt ttgt
//
LOCUS       DACDTO010000184         1591 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.191, whole genome shotgun sequence.
ACCESSION   DACDTO010000184 DACDTO010000000
VERSION     DACDTO010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.191"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            410..817
                     /locus_tag="IWK70_002899"
     CDS             410..817
                     /locus_tag="IWK70_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615373.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            831..1232
                     /locus_tag="IWK70_002900"
     CDS             831..1232
                     /locus_tag="IWK70_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615374.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1234..>1591
                     /locus_tag="IWK70_002901"
     CDS             1234..>1591
                     /locus_tag="IWK70_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615375.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKII"
BASE COUNT          599 a          202 c          273 g          517 t
ORIGIN      
        1 gccgccttcg ttatttaaat agtctgaaac tacttttttc ttaaaaacaa atgtatattt
       61 ccgcaaaaaa agcacccctt ttaattagat ttctagtcta acttttgggg tgcacatcat
      121 ttttgagggg aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt
      181 agtaaaactg tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac
      241 ttttactact gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa
      301 attagctaat atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca
      361 aaagtgaaac ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag
      421 agtacaatta ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa
      481 taaagtaatt aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt
      541 ttctgtaaca gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat
      601 tgaagtttta aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt
      661 gcctcctcaa gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga
      721 agcaggagag cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt
      781 tgatatcaag gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata
      841 tagtaccatc aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt
      901 gcgcatcggt aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta
      961 aaaatgataa tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg
     1021 atggcaaaat ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa
     1081 aatatgatga agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt
     1141 cctcagatta cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg
     1201 ggaaatctgt agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct
     1261 atttccaaac aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact
     1321 gctgtttcaa gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat
     1381 tatgatatta tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg
     1441 ttttcaacag ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta
     1501 gagaaaagaa aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg
     1561 ctgatgaatc gagaagcaga aaaaataata a
//
LOCUS       DACDTO010000185         1554 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDTO010000185 DACDTO010000000
VERSION     DACDTO010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.192"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            44..1510
                     /locus_tag="IWK70_002902"
     CDS             44..1510
                     /locus_tag="IWK70_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9615376.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          430 a          290 c          327 g          507 t
ORIGIN      
        1 tgtataatat tttctgaaaa tttaaagaat tggggtatag attatggaaa aacagcaaga
       61 caagggcttc ttcgggcagc cgaagggatt gtcaactttg ttcttcactg agatgtggga
      121 acgatttagt tattacggta tgcgtgcatt acttatctat tatatgtacg atacagttgc
      181 caacggggga cttggattac cacgagcaac agcattagct atcatgtcga tctacggatc
      241 tttgatctac atgtctagta tcgtcggcgg atgggtatct gaccgagtgt taggagcaaa
      301 acgcactgta ttcttcggtg gagttttcat tatgcttggc cacatcgtct tggctttacc
      361 atttggtgta agtacattgt ttgtttcgat ggcattgatt attatcggaa ccgccttttt
      421 gaaaccaaat gtttctggca tggtcggaca tctttattca aagactgact tgcgaagaga
      481 tgcaggattt tcgatttttt acatgggtgt caacttaggg gcattattag caccactaat
      541 cgttggtaca atcggtcaag aatataacta tcatttaggg ttctctttag ctgctattgg
      601 gatgttcttt ggattattac aatacatcat ccaaggacgc aagacattag acggagtggg
      661 tacaacaccg ccgaatcctt tgacaactga agaacgtaaa ggattcatcc gtaacttgat
      721 catcgcactt gttgttatcg ctgcagtatt cggcggggca caactaactg gtcatctgac
      781 aattgacttt tttgtcaata caatcagtgt attggggatt atcttaccgt tgtattactt
      841 tatcaaaatg ctgacttcaa aagatgtaac tgctgaagaa aaaccaagag tatgggctta
      901 tatcccatta ttccttgcag caatcatttt ctggtcatta caagaacaag gttcatctat
      961 cttagcttta tttgctagtg aacgtacaca atctacgcta tttggtgtaa cgatccaagc
     1021 aagctggtat caatcattga atccaatttt catctttatt ctgacaccag tattcgtaac
     1081 gatgtggacg aaacttggtg aacgtcaacc atctacagta gtgaaattct ctcttggact
     1141 tttatttgca ggattatcgt acttgctatt gatgttgccg ggtatgcttt atggtacaag
     1201 tggaaaagta agtccattgt ggttgttggg aagtttcttc atcgtggtta ttgctgaatt
     1261 gtgtctttca ccagttggtt tgtctgtgac aacaaaatta gcaccacgag catttgaatc
     1321 acaaacaatc gctatttggt tcttagctga tgcttcttca caagcaatca atgcacaaat
     1381 tgcacgcttc tacacaccgg gaacagaatc tgcttacttc ggtattatcg gtgcagtagc
     1441 aatcgtcggt ggtatcttgc tatttactgt gaaagaacca atcaaaaaac taatgggctc
     1501 tattcattaa tcaatgaaca taaaaacagg aattgcttat ttgcagcagt tcct
//
LOCUS       DACDTO010000186         1540 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDTO010000186 DACDTO010000000
VERSION     DACDTO010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.193"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..626)
                     /locus_tag="IWK70_002903"
     CDS             complement(<1..626)
                     /locus_tag="IWK70_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9615377.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPR"
     gene            914..>1540
                     /locus_tag="IWK70_002904"
     CDS             914..>1540
                     /locus_tag="IWK70_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9615378.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRN"
BASE COUNT          544 a          221 c          289 g          486 t
ORIGIN      
        1 tttctaggaa agcgcaggaa gtattgagtt agtttgtaaa gtctgcgatc ttcaaggatc
       61 gtaaatagct gatgggttgt cccatcaaca caaataaaac tcatggaacc agaaacagac
      121 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
      181 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      241 ttagcgatta gtttacgtga ttgattttcg tacagttcta tgagaatcgc ctgcttcagt
      301 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtctgccca
      361 caatcatagc atagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      421 ttcaatatct gagtacgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      481 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      541 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      601 agtaattttc tagtatgatt atccatagag cgactccttt ggtagggtga tgttttggtc
      661 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      721 gaaaatcgcc ttcgattttc caacaacaca aaatattata gaacccttta ttctaaagga
      781 tttcaaggtg tctgtttgca tttattagaa aaaagaaaaa gtattggcaa cagaaggtgt
      841 caccaacact agatattata gagccataat atgctgtata atatctagtg tataggctta
      901 agtggaggta tatatgaaaa tattaactgt tgaagatgat aatatgataa gagagggaat
      961 aagtgaatat ctttcagaat ttggatatac tgttattcaa gctaaagatg gaagagaagc
     1021 cttgtcaaaa tttaatagcg atataaattt ggttatctta gacattcaga taccttttat
     1081 aaatggttta gaagtgttga aagaaattag aaagaaaagc aatttaccaa ttctaatctt
     1141 gactgcattt agtgatgaag aatataaaat tgatgcattt actaatttag tagacggata
     1201 tgtagaaaag ccattttcat tgccggtctt aaaagctaga atagattctt taattaagaa
     1261 gaattatgga catttagaga agtttgaata taaggatcta tcggttaatt ttaatagcta
     1321 tacagctaaa ataaatggtg aagaaataga tgtaaatgca aaagaactag aagtattaaa
     1381 atgtttattg gataataatg gacaagtgtt aacaagaatg caaattattg attatgtatg
     1441 gaaagatagc gaagaaactc cttatgatcg agtaatagat gtatatataa aagaacttag
     1501 aaaaaaatta caattagact gtataactac tataagaaat
//
LOCUS       DACDTO010000187         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDTO010000187 DACDTO010000000
VERSION     DACDTO010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.194"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(46..130)
                     /locus_tag="IWK70_002905"
     tRNA            complement(46..130)
                     /locus_tag="IWK70_002905"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(94..96),aa:Leu,seq:caa)
     gene            complement(148..219)
                     /locus_tag="IWK70_002906"
     tRNA            complement(148..219)
                     /locus_tag="IWK70_002906"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(185..187),aa:Gln,seq:ttg)
     gene            complement(229..301)
                     /locus_tag="IWK70_002907"
     tRNA            complement(229..301)
                     /locus_tag="IWK70_002907"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(266..268),aa:His,seq:gtg)
     gene            complement(363..447)
                     /locus_tag="IWK70_002908"
     tRNA            complement(363..447)
                     /locus_tag="IWK70_002908"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(411..413),aa:Leu,seq:caa)
     gene            complement(468..538)
                     /locus_tag="IWK70_002909"
     tRNA            complement(468..538)
                     /locus_tag="IWK70_002909"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(504..506),aa:Cys,seq:gca)
     gene            complement(584..655)
                     /locus_tag="IWK70_002910"
     tRNA            complement(584..655)
                     /locus_tag="IWK70_002910"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(621..623),aa:Gln,seq:ttg)
     gene            complement(667..739)
                     /locus_tag="IWK70_002911"
     tRNA            complement(667..739)
                     /locus_tag="IWK70_002911"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(704..706),aa:His,seq:gtg)
     gene            complement(742..815)
                     /locus_tag="IWK70_002912"
     tRNA            complement(742..815)
                     /locus_tag="IWK70_002912"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(781..783),aa:Trp,seq:cca)
     gene            complement(822..902)
                     /locus_tag="IWK70_002913"
     tRNA            complement(822..902)
                     /locus_tag="IWK70_002913"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(866..868),aa:Tyr,seq:gta)
     gene            complement(904..979)
                     /locus_tag="IWK70_002914"
     tRNA            complement(904..979)
                     /locus_tag="IWK70_002914"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(944..946),aa:Phe,seq:gaa)
     gene            complement(1015..1087)
                     /locus_tag="IWK70_002915"
     tRNA            complement(1015..1087)
                     /locus_tag="IWK70_002915"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1052..1054),aa:Asp,seq:gtc)
     gene            complement(1097..1169)
                     /locus_tag="IWK70_002916"
     tRNA            complement(1097..1169)
                     /locus_tag="IWK70_002916"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1134..1136),aa:Val,seq:tac)
     gene            complement(1185..1256)
                     /locus_tag="IWK70_002917"
     tRNA            complement(1185..1256)
                     /locus_tag="IWK70_002917"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1221..1223),aa:Glu,seq:ttc)
     gene            complement(1271..1360)
                     /locus_tag="IWK70_002918"
     tRNA            complement(1271..1360)
                     /locus_tag="IWK70_002918"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1324..1326),aa:Ser,seq:gga)
     gene            complement(1400..1473)
                     /locus_tag="IWK70_002919"
     tRNA            complement(1400..1473)
                     /locus_tag="IWK70_002919"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1437..1439),aa:Asn,seq:gtt)
BASE COUNT          428 a          397 c          342 g          337 t
ORIGIN      
        1 aaaatatagc aactctctat aagaaagttg ctatattgat aattataccg gcggccgggg
       61 tcgaaccggc acgtcctcaa cggacactgg attttgagtc cagcgcgtct gccaattccg
      121 ccacgccggc aaatttaaaa ataataactg gggtagctgg attcgaacca acgcatgagg
      181 gagtcaaagt cccttgcctt accgcttggc tataccccaa gaataaaggg cgaatgatgg
      241 gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg
      301 ccatcataaa agatgttatt taatttctca ataaaaaact aagatgaaga catcttagtc
      361 aataccggcg gccggggtcg aaccggcacg ccctcaacgg gcactggatt ttgagtccag
      421 cgcgtctgcc aattccgcca cgccggcaag ttgatttatt agtactaagg cggtaaccgg
      481 atttgaaccg gtgatgaagg ttttgcagac ctctgcctta ccacttggct ataccgccat
      541 tatactatta tcaatctata aaataaagaa taaattatag aaactggggt agctggattc
      601 gaaccaacgc atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat
      661 aaaaagggcg aatgatggga atcgaaccca cgaatgccgg agccacaatc cggtgcgtta
      721 accacttcgc cacattcgcc atggcagggg cagcaggaat tgaacccaca ctaacggttt
      781 tggagaccgc tgttctacct ttaaactatg cccctataaa atggaggaga gtggattcga
      841 accactgaac cctaaggaac ggatttacag tccgtcgcgt ttagccactt cgctactcct
      901 ccatggtggc gcgggacaga atcgaactgc cgacacacgg agcttcaatc cgttgctcta
      961 ccaactgagc taccgcgcca tattttaaaa ttattaattg aataacaaga acgacggttc
     1021 cgacgggaat cgaacccgcg atctcctgcg tgacaggcag gcatgttaac ccctacacca
     1081 cggaaccagt tcattatgga ggttaacggg atcgaaccgc tgaccctctg cttgtaaggc
     1141 agatgctctc ccagctgagc taaacctcca acattcaatt aaaatgaccc gtacgggatt
     1201 cgaacccgtg ttaccgccgt gaaagggcgg tgtcttaacc gcttgaccaa cgggccaggt
     1261 aaatttgcta cggagagtaa gggattcgaa cccttgagac agtttggcac catctacatg
     1321 atttccaatc atgctccttc ggcctctcgg acaactctcc agagtgaaag aagctcaata
     1381 ctctttctca aaatgagaac tccggcagta ggactcgaac ctacgacatc atgattaaca
     1441 gtcatgcgct actaccaact gagctatgcc ggaataacat cagcgtggcg acgtcctact
     1501 ctca
//
LOCUS       DACDTO010000188         1490 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDTO010000188 DACDTO010000000
VERSION     DACDTO010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.196"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..948
                     /locus_tag="IWK70_002920"
     CDS             <1..948
                     /locus_tag="IWK70_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9615379.1"
                     /translation="FRCKHCYNASGGDNCFMDNELTDDELLELTKDICNLKPYNICFC
                     GGETLLRKKIVLSCATLLKEAGIPNIAIVTNGSLVTQDVAKELFKAGINRFQVSLDGK
                     NASSHEALRLYPNSFEKAINAIKLFRELSEEVDVAFCPTSFNYNQLREVYQLVNTLKV
                     KSLRIQPFMLSGRGMQNREIVPTVSQYRKLVSDINYIKRNEDYTKIDWGDPIDHIIRH
                     RTLLKDFSNFVSIRSDGGLMASPYLPIQVGNIKCHSLSKYWRAGLYKVFSLDLVQDIA
                     TQVSSIQDMSKELKKGHIKWLNEDIEIDILDEYKHDILI"
     gene            1004..1222
                     /locus_tag="IWK70_002921"
     CDS             1004..1222
                     /locus_tag="IWK70_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615380.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
BASE COUNT          556 a          178 c          286 g          470 t
ORIGIN      
        1 tttagatgta aacattgtta caatgcgagt ggtggagaca attgttttat ggataatgag
       61 ttgactgatg atgaactgtt agaacttaca aaagatatat gtaatttaaa gccatataat
      121 atttgtttct gtggaggaga aactttactg agaaaaaaaa tagttttatc ttgtgccact
      181 ttattaaaag aggcaggtat tcctaatatt gctattgtta ctaatggatc attagtaact
      241 caggatgttg caaaagaatt attcaaagca ggtattaatc gatttcaagt aagtttagat
      301 ggaaaaaatg ctagtagcca tgaagcactt agattatatc caaacagttt tgaaaaggca
      361 attaatgcta tcaaattatt tagagaattg tccgaagaag tggatgttgc tttttgtcct
      421 actagtttta attacaatca attacgagaa gtatatcaac tagtaaatac tcttaaagtg
      481 aagtctttaa gaattcaacc atttatgctt agtggtaggg gaatgcaaaa tagggaaata
      541 gtgccaactg tttctcaata tagaaaacta gtttctgata ttaactatat taaaagaaat
      601 gaagattata ccaagataga ttggggagat ccaattgatc atatcatccg acacagaact
      661 ttattgaaag atttttcgaa ttttgtttct attaggtcag acggaggatt aatggcatca
      721 ccttacttgc caattcaagt tggaaatatt aaatgtcaca gtctttctaa atattggcga
      781 gcaggacttt ataaagtttt ttctttagat ttagttcaag atatagctac tcaagtttct
      841 tcaatacaag atatgagtaa agaattaaaa aaaggacata ttaaatggct taatgaagat
      901 atagaaattg atattctcga tgaatacaaa catgatattt taatttaggt ttatcattgg
      961 aaagtttaga taaacaaagt acttttagaa aggagggaaa aaaatgattt ctgaacctca
     1021 aatgaataat atgtatgaaa gcccaactat aatgggagct ggtgaagctg cgccaagagc
     1081 ttgttcagca attatggtgt gtgtgttatt agcatctgca ggagtaggta tccaagcagg
     1141 agcggtgtat acttatgctg tagttgctac agtagctgta gctgcagccg cagtttggaa
     1201 aacagtagga ttagttggtt aggaattaat aggagactaa ctgtctccta ttgattccaa
     1261 tgattaagct aaggaggaat tttaagaaaa taatgaaata caataattta aaaccgaaat
     1321 tacttaatga gaacatagaa tatataagag aagaaaatta tggatattta accatagttc
     1381 ccaaaaatgg aactgaattg aaagataacg ttttcaatcc aacagtaaga tatatattgg
     1441 ctaactgtac tggaaaaaac agtgtaaaaa aaatagcgaa tggtttaaaa
//
LOCUS       DACDTO010000189         1477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDTO010000189 DACDTO010000000
VERSION     DACDTO010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.197"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            138..752
                     /locus_tag="IWK70_002922"
     CDS             138..752
                     /locus_tag="IWK70_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9615381.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(917..>1477)
                     /locus_tag="IWK70_002923"
     CDS             complement(917..>1477)
                     /locus_tag="IWK70_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615382.1"
                     /translation="NTRDRFISIITACEIGVKEFYQQEKPDLTLIFENLQSPPVDKLL
                     GSMFKGYFNIEFPKEIRKEIKNFIEKRNKLVHSPQGSKPTIEECFECYMAVLKTFNFF
                     YKINDSFMYRDFYEESIELTPTEPNKSQITLSKELERKVESQELAIGISITNVSTYKE
                     LYEAKSQDNKDFVENIDKEPTNTVGD"
BASE COUNT          485 a          261 c          228 g          503 t
ORIGIN      
        1 atactatttc ctccatcatt agtttttgtc tatataaggt ataccctaac tagacacgac
       61 cgcgacattg aaaaatctgg ttttctcgtg tatgattagg gtgtttttta aatagttgca
      121 gaaagtaggt aaaaattttg agagttgcat acatacgtgt ctcatccatc gatcaaaatg
      181 aacaacggca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc attgagaaac
      241 aatctggcgc aacgattact catcgacctg tttttcaaga agcactggat ttcgtccgag
      301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattac gacgagatca
      361 tcgcttcagt caattacttg aagaagaaaa atgtgcagct gatcatcaca agtctaccga
      421 tcatggccga agcaattggc aatcctctcc tggataaatt cattaaagat ttgatcattc
      481 aaattttggc catgatcgct gaacaagaac gtaccgaatc taaacgacgt caggcacaag
      541 gcattaagat tgcaaaagcg aacggtgttt ataaaggacg tcctaagcta tacagtgcca
      601 atgcaaaaga tccccaacgt cgtcttgttt ataaaaacat cgttgaggat ctaaaaaaag
      661 gtgttgcgat cgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
      721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
      781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
      841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
      901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
      961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     1021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     1081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     1141 ctctatacat aaagctatca ttaattttat aaaaaaaatt aaatgttttt agcactgcca
     1201 tataacattc aaaacattct tctatagttg gtttacttcc ttgaggagaa tgaaccaatt
     1261 tattacgttt ttctatgaaa tttttaattt cctttcttat ttctttcgga aactcaatat
     1321 taaagtaccc tttaaacatg ctccctaata atttgtctac agggggagat tgtaaattct
     1381 caaatattag agttaaatca ggtttctctt gttgataaaa ttcctttacc cctatttcac
     1441 aggctgtaat tatcgaaata aacctatctc tagtatt
//
LOCUS       DACDTO010000190         1475 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDTO010000190 DACDTO010000000
VERSION     DACDTO010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.198"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(870..1127)
                     /locus_tag="IWK70_002924"
     CDS             complement(870..1127)
                     /locus_tag="IWK70_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9615383.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(1120..1389)
                     /locus_tag="IWK70_002925"
     CDS             complement(1120..1389)
                     /locus_tag="IWK70_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9615384.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT          487 a          257 c          161 g          570 t
ORIGIN      
        1 gagaaaaggg gatactttcg tgtccatgca tgtaattaac taattgttga gcttcagaag
       61 ttatatctct aattatatag ttaacggaat taggaagtgt ctttaactct aatgaaattt
      121 ctatccaatt actatcttct aatctgtata tgctcgaagt ataatttgat tcgtcagcaa
      181 gcatcgctac atcccaaaca ctgatcataa aatcttttaa acgttcaatc atttcagcaa
      241 tattgctaaa atcagctaat ttttctttag aataaaaaaa acatttatat cagaatcaat
      301 aaattctatt ttttttatta ctatatcgtc atcattatct tgaccattta tgtttattgt
      361 tccctgagtg gggagtactt ctttcaaatt caagttaaat ttatattcca ttacttctcc
      421 tccaatatat aaaaattact taactgtatc caaatttaat atgtaaataa atcattaatt
      481 tttagctaaa ttctatgaga agtttatttt tgttgttttt gctaattaac taaaactagt
      541 acaaaaaatt taaaatagat tattaaacct attgcttatt ctattcagtc atatatataa
      601 gcgtcatcct ttagtaacta acatcccaca ctacaatagt attataacat atctattcaa
      661 cttcaaattg ttgaaattta agccatttat atttactttt cctcaacttt agaaatgatt
      721 ttactaacct tatatccgta atacgcgtaa caaacacatt cattaataac aatcaacact
      781 aagctatatt agctttttaa atgactaggg taaataaaat aaggatcaaa aacttttaag
      841 tttctgaccc tttccttact tccgattggt taatagtgat cttttgcaga ataaataaat
      901 atcgtttcat tttcaactct atatatcagt ctatgttcat ctgtaattct tctggaccat
      961 tttccagata aatcatgctt taatggctca ggttttccta atccagcaaa gggggaacga
     1021 tcgatatctt ttattaactt gttaatcttt tttatattgc ttttgtttcc ttgctcatgc
     1081 caataaagat aatcatccca agcatcatca gaccaagcct taatcatcag attcaacctc
     1141 gattaagtca tgtgttttaa atgcaccttt ggagaattgt tcatctcctc gacgaatttt
     1201 ttccatgacg taattattag aaagtgttct caacgtttct tgcatagaat cataatctct
     1261 ttttgataat acaacaactg tatcttctac atctttactt gttacaataa gtgtttcagc
     1321 atcctcatta acttgtttca tataactacg taaattttgg cggaaatttg aataagctac
     1381 tgcttccatt cctttcaccc cttatttatt tctataaaac aattgtacat tatattgtac
     1441 aattaagcaa atgattaata ttctaacttc ttata
//
LOCUS       DACDTO010000191         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDTO010000191 DACDTO010000000
VERSION     DACDTO010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.199"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IWK70_002926"
     CDS             complement(<1..625)
                     /locus_tag="IWK70_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9615385.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IWK70_002927"
     CDS             complement(784..1050)
                     /locus_tag="IWK70_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9615386.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IWK70_002928"
     CDS             complement(1040..1396)
                     /locus_tag="IWK70_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9615387.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          405 a          279 c          181 g          586 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg a
//
LOCUS       DACDTO010000192         1435 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.200, whole genome shotgun sequence.
ACCESSION   DACDTO010000192 DACDTO010000000
VERSION     DACDTO010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.200"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWK70_002929"
     CDS             105..1406
                     /locus_tag="IWK70_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9615388.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          473 a          287 c          264 g          411 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgtt
//
LOCUS       DACDTO010000193         1417 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDTO010000193 DACDTO010000000
VERSION     DACDTO010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.201"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWK70_002930"
     CDS             complement(150..1337)
                     /locus_tag="IWK70_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9615389.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          412 a          308 c          249 g          448 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttc
//
LOCUS       DACDTO010000194         1393 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDTO010000194 DACDTO010000000
VERSION     DACDTO010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.202"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>1393
                     /locus_tag="IWK70_002931"
     rRNA            <1..>1393
                     /locus_tag="IWK70_002931"
                     /product="23S ribosomal RNA"
BASE COUNT          374 a          300 c          424 g          295 t
ORIGIN      
        1 aagtctgaag aggagtcaaa tgcttctttt cttaaggaca agctgtgatg gggagggaaa
       61 taatagtacc gaagttcctg atgtcacact gccgagaaaa gcttctagtg agaaaacagc
      121 ggcccgtacc gcaaaccgac acaggtagtc gaggagagaa tcctaaggtg agcgagagaa
      181 ctctcgttaa ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgatcat
      241 acgatcagcc gcagtgaata ggcccaagcg actgtttatc aaaaacacag gtctctgcaa
      301 aatcgtaaga tgaagtatag gggctgacgc ctgcccggtg ctggaaggtt aagaggagtg
      361 cttagcgcaa gcgaaggtac gaattgaagc cccagtaaac ggcggccgta actataacgg
      421 tcctaaggta gcgaaattcc ttgtcgggta agttccgacc cgcacgaaag gcgtaacgat
      481 ttgggcactg tctcaacgag agactcggtg aaattttagt acctgtgaag atgcaggtta
      541 cccgcgacag gacggaaaga ccccatggag ctttactgta gtttgatatt gagtgtctgt
      601 accgcatgta caggataggt aggagccgta gaaatcggaa cgctagtttc gatggaggcg
      661 ctggtgggat actacccctg cgttatggcc actctaaccc gcaccactaa tcgtggtggg
      721 agacagtgtc agatgggcag tttgactggg gcggtcgcct cctaaaaggt aacggaggcg
      781 cccaaaggtt ccctcagaat ggttggaaat cattcgaaga gtgtaaaggc agaagggagc
      841 ttgactgcga gaccaacaag tcgagcaggg acgaaagtcg ggcttagtga tccggtggtt
      901 ccgcatggaa gggccatcgc tcaacggata aaagctaccc tggggataac aggcttatct
      961 cccccaagag tccacatcga cggggaggtt tggcacctcg atgtcggctc gtcgcatcct
     1021 ggggctgtag tcggtcccaa gggttgggct gttcgcccat taaagcggca cgcgagctgg
     1081 gttcagaacg tcgtgagaca gttcggtccc tatccgtcgc gggcgttgga aatttgagag
     1141 gagctgtcct tagtacgaga ggaccgggat ggacttaccg ctggtgtacc agttgttctg
     1201 ccaagggcat tgctgggtag ctatgtaggg aagggataaa cgctgaaagc atctaagtgt
     1261 gaagcccacc tcaagatgag atttcccatt tctttaagaa agtaagatcc ctgagagatg
     1321 atcaggtaga taggtcagga gtggaagtac agtgatgtat ggagcggact gatactaatc
     1381 gatcgaggac tta
//
LOCUS       DACDTO010000195         1373 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDTO010000195 DACDTO010000000
VERSION     DACDTO010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.203"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            191..445
                     /locus_tag="IWK70_002932"
     CDS             191..445
                     /locus_tag="IWK70_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9615390.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IWK70_002933"
     CDS             446..763
                     /locus_tag="IWK70_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9615391.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          418 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DACDTO010000196         1372 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDTO010000196 DACDTO010000000
VERSION     DACDTO010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.204"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            122..>1372
                     /locus_tag="IWK70_002934"
     CDS             122..>1372
                     /locus_tag="IWK70_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9615392.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          428 a          271 c          261 g          412 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagttatacc aagacattct
     1141 ttatgcggta aagaaaagag atcagtcact atttgaggat tgtttaacta gagacattag
     1201 cggtctgcca gaaacctata caaccacttt acgaacgttt aaaaagtttc tgccacagat
     1261 ccaaaatgcg cttcactaca gctattcaaa cggaccattg gaatgcttaa acaaccacat
     1321 caaagtatta aaacgcaatg cgtacggctt tcgcaacttt tacaatttca aa
//
LOCUS       DACDTO010000197         1348 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDTO010000197 DACDTO010000000
VERSION     DACDTO010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.206"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(124..1272)
                     /gene="tnpB"
                     /locus_tag="IWK70_002935"
     CDS             complement(124..1272)
                     /gene="tnpB"
                     /locus_tag="IWK70_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9615393.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTIALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          389 a          237 c          213 g          509 t
ORIGIN      
        1 taacgcttga agtgggagct tcttgggaat agagcatact tgatagcgta tttctttact
       61 aacagcggtg tctcttattt accaagctat ccccgtagtt cctacggttc ttgatgattg
      121 gatctaagcc aatcccatag aatgtagtct tagaccttcg gtcagaatat tgatactagc
      181 attgatatca cgatcatgat gagtatgaca aacaggacaa gtccattctc gaatttcaag
      241 agatttcttg ccatctttat gtccgcactc tgaacaaatc tgactagacg gaaaccattt
      301 atctactttg atgatttttc gtccatacca gtcagccttg tattgtaatt tagtcacaaa
      361 ctttgaccaa gaaacatcag aaatactttt agctaattta tgatttcgca acataccttt
      421 tgtgtttaag tcttcaatac agataatatc gtgatttttg atcatttctg tactcaactt
      481 attcagaaaa tcagtacgtt gattcattac tttttcatgc aatctagcta ctttgcgttt
      541 ttgtttttga taatttttag cttcaaatag attggtacct ttatttttag ccaataaagc
      601 acgcctagac aatttgcgtt gttcacgctt tagtttcttt tccattttgg acgtgaattt
      661 attattatca attttttgtc catcagaaag aatcgcaaag tccataatac ctaagtcaat
      721 accaattgtg gaattagttt taggtaattc gataatttct tctttacaca acaaagaaat
      781 atagtattta ccgctagaat gacgtgatat tgtagcagat ttgataatcc cttttggttg
      841 tctatgaagc ttaattctta ctaatgactt caatttagga actttgatga atttactatc
      901 aatcaaagca attgtaccat tttgattatt cgttgtataa ctctgaacag gatttttctt
      961 acttttgaaa cgtggaaatc caacggattt atcccgaaaa aaattcttgt atgctttatc
     1021 taaattaagt tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa acggaaattc
     1081 tttcttgtat ttagctggtg tcggaaaagt cattttttta gaagaatcat ttttaacttc
     1141 ttcatacgcc ttttttcggt catcaagcat tagattgtag accttacgga cacaaccaaa
     1201 agatttggca aagaagattt cttgttcctc tgttggatag attctgaatt tgtatgcttt
     1261 tagttgttcc ataaagttca cctactttct atttatgatt gtttccttga ttttggatat
     1321 acttcttaat cacatcaatt ggtgcgcc
//
LOCUS       DACDTO010000198         1347 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDTO010000198 DACDTO010000000
VERSION     DACDTO010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.207"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..634
                     /locus_tag="IWK70_002936"
     CDS             <1..634
                     /locus_tag="IWK70_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615394.1"
                     /translation="NKNSIFKKKIDDYFVYCPELLLYDVKNIKKIPKIDENSKLMKNI
                     QSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKVNSIFSGKKETLIKLHPRART
                     NRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISFSPSMMFEKTINSIMLAKLIL
                     NEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENWAELRKLVEYGII"
     gene            621..>1347
                     /locus_tag="IWK70_002937"
     CDS             621..>1347
                     /locus_tag="IWK70_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9615395.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMI"
BASE COUNT          462 a          174 c          198 g          513 t
ORIGIN      
        1 aaataaaaat agcatcttca aaaaaaaaat tgacgattat tttgtctatt gtccggaatt
       61 gttactttat gatgtaaaaa atataaaaaa aattcccaaa atagatgaaa acagtaaatt
      121 gatgaaaaac atacaatcaa tctttgggat agaagaagtt tttgtcggag ataaatgtat
      181 cttttttgat caacctttat tatctgatgg ttatggcata gatgaaacag atattttcgc
      241 taaagtaaat agtatatttt ctggaaaaaa agaaacttta attaagcttc atcctagagc
      301 aaggacaaat cgttatggtg aaaaaaacat attgaattcc agtttaccgt ttgaagtctt
      361 gctacttgaa aatgacttct cttcaacact tttgattagt cctatatcta caatttcttt
      421 ttcaccatct atgatgtttg aaaagacgat caactctatt atgttggcta aattaatttt
      481 aaatgaatat acagcattat ctgaagataa aaaagaaaca ttgacaaata ttgttctttt
      541 ctgtgaacat tataaccgtc tatcagaaga aaaaatttta ctcccagaaa actgggcaga
      601 attaagaaag ttggtagagt atgggattat ttaacacact ttccaagtca aaattagtta
      661 agtccagttt ctggtatact atagggaatt tttttattaa gggaatttca ttcataacta
      721 ttcctatttt tgtaaatatt actactcaag cagaatatgg tttgattaat aatttcactt
      781 catttgtatc aattttttcg attcttgttg ggctaagcct aaattcggca attaataatg
      841 caagttttga ctataaagat gacataaatg aatttatgtc atcggtactt tctctagcta
      901 cactttcgtt aattgtgttc ttattaatag ggaacatata ttttttgttt tcaaatgctt
      961 tttttgatat gaaccaactt gtatttaatt tgatgttatt acaaagtttt gctagttttg
     1021 taattagtta tatccagtct tattttacga ttaactttaa ccattttaaa tatttgattt
     1081 tatcttttat atctactttt ttgaatattg caatctctgt agctttgatg cagacattat
     1141 attttgatga taaatatgtt ggcagagtag tcggcggaac actgtcattt gtgttgatag
     1201 gtttagtaat ttatttaaga ataatgataa agggtgctca tttttttgat aaaaaacagt
     1261 ggagatacgc tttaaaaatc tctttgccgc taatcccaca ttccttagca aatatagttt
     1321 tagcacaatt cgatagaatt atgatta
//
LOCUS       DACDTO010000199         1346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.208, whole genome shotgun sequence.
ACCESSION   DACDTO010000199 DACDTO010000000
VERSION     DACDTO010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.208"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            68..>1346
                     /locus_tag="IWK70_002938"
     CDS             68..>1346
                     /locus_tag="IWK70_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP9615396.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          456 a          255 c          257 g          378 t
ORIGIN      
        1 aaaacatatc gttctctagt atgattaaat cacgacaaaa taaacaactg agaggacgat
       61 atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag atttgatgat
      121 tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg atgctgtttt
      181 ctcacctgct ccccatacct gtagaaattg tggttctact gtggtagatg gaaacgggaa
      241 agtaattgta gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac aatacaatca
      301 catgcctttg gttatgcgct taaaaaagca gcgctatacc tgtaaaaact gtcgaaccca
      361 ttggacggct caaagttatt ttgttcaacc cagacattca atcgcgaatc atgtcagata
      421 taaaattgct tctttactga ctgaaaaagt atctttatct tttattgcga aaaattgtca
      481 ggtatctttg accaccgtta ttcgtacgtt gaaagagttt aaaagctact taccaaagca
      541 atctaagagg attctcccca gagtattgat ggttgatgaa tttcgttcgc acgcttccat
      601 agaagataaa atgagcttta tttgcgcaga tggcgaaaca ggacaattaa ttgatgtctt
      661 gcctacgcgt aaattgcctc gattaacaag ctatttctta ggctgcgcta atcctgaaga
      721 agtaaaattt ttagtcacag acatgaacgc cgcctacttc cagcttacca aacgtattct
      781 gccaaatgcg aaagtagtga ttgatcggtt tcatattatc aaacacatga atcaagcctt
      841 taatgagttg cgtatccgtg aaatgaatga acttcgtaaa gtaggacaga aaagtcaggc
      901 agaaaaactg aaaaagaact ggcgcttttt gctaaaaaat cgtgcaaaca tcaaccatta
      961 tgaatacaaa acatggaaaa gttttcgagc accaaaatac ccatttctta cggaagcgat
     1021 gatgattgat cgattgcttg gcttttctac gtccctgaag gaggcgtatc cctattttca
     1081 tgaattagtt gaagcctttc gagacaaaga ccctgactta tttttctcct tattggcaga
     1141 acttcccgaa acgttagatg acggctttcg ggaaaagctc caaaaccttc tgacctatga
     1201 agaaggcatc accaacgcga tgatctatcc ttacaccaat ggaaaaatag aagcgaagaa
     1261 tacccatata aagacaatga aacgagtatc ctacggattt aaatcatttg agaacatgag
     1321 aattagaatc tttttgatca atcaat
//
LOCUS       DACDTO010000200         1322 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDTO010000200 DACDTO010000000
VERSION     DACDTO010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.209"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWK70_002939"
     CDS             7..624
                     /locus_tag="IWK70_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615397.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          499 a          169 c          206 g          448 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ct
//
LOCUS       DACDTO010000201         1300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDTO010000201 DACDTO010000000
VERSION     DACDTO010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.210"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            18..>1300
                     /locus_tag="IWK70_002940"
     CDS             18..>1300
                     /locus_tag="IWK70_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9615398.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTK"
BASE COUNT          405 a          283 c          261 g          351 t
ORIGIN      
        1 caaaggagtg tttcaagatg tcctattcag agtttaccaa agagttacta gatattctag
       61 acctaaatct tacctttcat gaagatgcct ttcgcaaaga gcgcattaat gacgaaacat
      121 gttttgtttt tgatggcaca ttgacctatc aaccagagga atgcttccat tgccactatc
      181 aaaataagca aacgatcatt aaatggggct ggaaaaaagt atccatctta ttgaatgatg
      241 tcagtaacta caagaccatt gtgcggatta acaaacagcg cttcaaatgt aaacactgtg
      301 gaaagacttt tttagcagaa gattcagtta gcgatcgtcg ctgctccatt gcccgcagag
      361 tcaaacaagc cattcttgag ctattaagtg agcctatttc tatgtctttg attgcacgta
      421 tgaagcacat ttctccgacc actgtcattc gaatacttcg cagccttcgg cctaaaaccg
      481 tttctttgaa ccagccgtta ccagaagtgg tgtgtttcga tgaattcaaa tcggtgaaaa
      541 acgtctcggg agccatgagc tttgtcatga tggacggcaa gacccatcaa ctgattgata
      601 ttgtggaaaa tcggcaactc aatcctttac gcgactattt cttgcgctac cctagaaaag
      661 tccgtgaaca agtacgtcta gtggtttcag atttctatac accctatcgc acattggtga
      721 aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt tcatattagc caacacatcg
      781 gtcgggcctt taccaaccac cgaattcaag taatgaagac ctttaaaaaa ggggatcgtc
      841 ggtcgaagca cttaaagaaa tactggagac tacttcaaaa gaatgcgtgg gaattgaatg
      901 gtcagcatcg ctactggcgt ccgtcatttc gagatcatct gacagaagcg gagatagtcg
      961 atcggctatt atattatgat gactccttaa agcgaggcta cgaagtctac caggtttttc
     1021 tttccgctat cagaagacaa gatgttcctg aattcgtcgc gctcctaaaa gaagactaca
     1081 aggagttgcc agagcactat caaccagtgt tcacgacctt caagaaatat cgaacagaaa
     1141 tcaaacgagc gttgcgagtt ccctattcca acggccctat agaatgtttg aacaaccata
     1201 tcaaagtact aaaacgaatt gcctatggct ttagaaactt tcaaaactac cgagaacgaa
     1261 tctttttata tcgaggaaaa tatttcaaga aaactaagaa
//
LOCUS       DACDTO010000202         1269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDTO010000202 DACDTO010000000
VERSION     DACDTO010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.211"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            24..1103
                     /locus_tag="IWK70_002941"
     CDS             24..1103
                     /locus_tag="IWK70_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9615399.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          302 c          310 g          279 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg
       61 gaaaacatct ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt
      121 ccaaccgtca ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg
      181 atcgcggaac catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct
      241 actattctgt ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact
      301 gtggcagacg ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa
      361 tgttaaatga aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt
      421 tcgattgtgc catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca
      481 tgcgaacaaa gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc
      541 caggacaccg tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag
      601 ttgaaacacg ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca
      661 agtcggatgc cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa
      721 agttgaacgg gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg
      781 ctggcgctaa cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt
      841 ctgggctaca cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt
      901 cttgggagcg tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg
      961 gaagcccgat tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg
     1021 attacccgcg gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca
     1081 agcaagaacg gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat
     1141 ggcttcatgt ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag
     1201 ttcattatca aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga
     1261 gttacctgt
//
LOCUS       DACDTO010000203         1258 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDTO010000203 DACDTO010000000
VERSION     DACDTO010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.212"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            188..391
                     /locus_tag="IWK70_002942"
     CDS             188..391
                     /locus_tag="IWK70_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615400.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="IWK70_002943"
     CDS             602..1186
                     /locus_tag="IWK70_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9615401.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          447 a          177 c          244 g          390 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca cttccttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgta
//
LOCUS       DACDTO010000204         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDTO010000204 DACDTO010000000
VERSION     DACDTO010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.213"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWK70_002944"
     CDS             200..742
                     /locus_tag="IWK70_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615402.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IWK70_002945"
     CDS             832..>1246
                     /locus_tag="IWK70_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9615403.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDTO010000205         1245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDTO010000205 DACDTO010000000
VERSION     DACDTO010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.214"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1244
                     /locus_tag="IWK70_002946"
     CDS             <1..1244
                     /locus_tag="IWK70_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9615404.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          410 a          222 c          268 g          345 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaa
//
LOCUS       DACDTO010000206         1236 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDTO010000206 DACDTO010000000
VERSION     DACDTO010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1236
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.215"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            82..>1236
                     /locus_tag="IWK70_002947"
     CDS             82..>1236
                     /locus_tag="IWK70_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9615405.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAM"
BASE COUNT          411 a          242 c          239 g          344 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatg
//
LOCUS       DACDTO010000207         1231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDTO010000207 DACDTO010000000
VERSION     DACDTO010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.216"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..634)
                     /locus_tag="IWK70_002948"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="IWK70_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          391 a          197 c          207 g          436 t
ORIGIN      
        1 aggtaaaaaa taagtctgat tccttgtctc taaaggcttc ggtcagctca tggaaaaaag
       61 tataggcgtc ctttaaaggc gttgaaaatg ccagcaatcg atcaatcatc attgcttcag
      121 tgagtagcgg gtatttaggt gcacgaaaac tgcgccatgt tttgtaatca tagtgattga
      181 ttttttgacg gttttttaga agaaagcgcc agttcttttt cactttctcg gcttcccgtc
      241 gttgccctaa ctggtgtaat cttttcattt cttgtacacg aaaatcattg aacgcctgat
      301 tcaaatgttt gatgatatga aaacaatcaa tgacgacttt cgcatttggt aagacacgtt
      361 ttgtcagttg gaagtaggcg gcgttcatat ctgtcactaa atattccacc gcttcaggat
      421 ttgcacagcg actaaaatag tgtgttaatc gagacaattt tcgtgtaggc aaaatatcaa
      481 tgagttttcc agtctcgcca tccgcacaaa taaagctcat tttatccaca attgaagcgt
      541 gcgaacgaaa ttcatccacc atcaatactt ttggcagggt cctttttgac ggcttcggaa
      601 gatagttttt caactctttc aatgtacgaa tcatatacct aaaataacaa tgggactgaa
      661 gaatcaattt tgattcatca gtcccataaa ttatagagcc tatattttgc gaattgcgct
      721 acggtttctt cttttctttt gtctgtaata tgaatatata gatccataat gatttgaata
      781 gaagagtggc ctaaacgctc ttgcacatct ttaatatttg cactagcttg ctaaaagcaa
      841 gctagcatgt gtgtgtctaa gaccatgaat agtaattcgt ttaagattgt ttttcctgat
      901 gattacatct aaccattttc gagatttaga taattgaagg tgttcatttc tttctggtaa
      961 aaatttaaat atataactac attttctcta ctaactttga aacactactt ttgtaatata
     1021 aaaaatattt tattaataat aaaattaatc acattttata tagtgagtaa tctagtgttt
     1081 taaaaaaata aatgatacta tatgtaatat aaaaaaacat gaggtggtat atgaatttga
     1141 aaaaaatttt ttctgtaaaa atatttatag tttatgcatt tgttagttta ttatatttat
     1201 ttccagcatt taaaaacatg gaaatatttg g
//
LOCUS       DACDTO010000208         1214 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDTO010000208 DACDTO010000000
VERSION     DACDTO010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.217"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IWK70_002949"
     CDS             <1..156
                     /locus_tag="IWK70_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP9615406.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>1214
                     /locus_tag="IWK70_002950"
     CDS             175..>1214
                     /locus_tag="IWK70_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9615407.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSI"
BASE COUNT          514 a          100 c          209 g          391 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taat
//
LOCUS       DACDTO010000209         1210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDTO010000209 DACDTO010000000
VERSION     DACDTO010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.218"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..1190)
                     /locus_tag="IWK70_002951"
     CDS             complement(<1..1190)
                     /locus_tag="IWK70_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9615408.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGI"
BASE COUNT          466 a          206 c          141 g          397 t
ORIGIN      
        1 attataccac ttgatacaag tacttgaaga aatccattat gcatgtttcc atgtgctctt
       61 tttagttcat tcacatctgt agtatcaaga tctacttttt tcaaaaattt tgcatcgcta
      121 ttcccataga aatcggtctc tccataacca aataacaaag ttggtcttat aactttaagc
      181 gcagctttcc aaatcgtaaa acgaccagct gaaatttcgg agttatcact agaatgctgt
      241 aatattactt ctttttctgg aagtggagtt ttatctgatt catttgactc tataacttct
      301 acttttctat caatcgatac aggaattgtt gctaaaaaat ccttagcata attagtacca
      361 aaagaaaaat ataccaatag aactaataat atgcttacca tccatttgat ttttacaggt
      421 agtacttctc taatgctctt tctaaatttt gaattggcaa acaacaaaag aaataatact
      481 aaaaaagaac aaattactac ctgggcacct ctagacgaag aaagtgtaat ataaaatatt
      541 tgtacgataa aattcatcat ataaaaaaca cgaatcgttt ttgttatttt tgaaattaat
      601 acactaatta aacttaaaat aatagaagta tatccaaaca tagctccaac atttggactt
      661 gtatagacac caaataaccg atgttcaata aaaccttgtc ttgcaagtaa tcctggcgtt
      721 cctgttggga catgataaga aatcaaaaaa ataaattgat agatagatat tagcgcagct
      781 aaaaaaatta tgataatgaa aacattatta aaatacacca tttttttctt tttttcctct
      841 tcactttgtg aaaagtctgc aggaaaaatt actatcaaag taatcagcaa atatccaaaa
      901 ttataaatag atggaattaa aaaattttct ctgttaatga ttatagttaa gataaaacta
      961 atcattgctc caatcagtag ccaagaaaaa cgcattttaa aaaaattttt ctttataaaa
     1021 acctcataca aaactataat tccaccccaa gcaaaagcta ttttcatcgg gatattagtt
     1081 acattaaaag tactaggcat aaaataaagc agacaaatga ttaaatttat tactcgaaac
     1141 gcatctcgac ttaataaact cttctctaaa acaggtacta ttctattcat ctatatttcc
     1201 aactttcaaa
//
LOCUS       DACDTO010000210         1197 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDTO010000210 DACDTO010000000
VERSION     DACDTO010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.219"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWK70_002952"
     CDS             <1..153
                     /locus_tag="IWK70_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9615409.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IWK70_002953"
     CDS             159..575
                     /locus_tag="IWK70_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615410.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IWK70_002954"
     CDS             681..944
                     /locus_tag="IWK70_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9615411.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..>1197
                     /locus_tag="IWK70_002955"
     CDS             944..>1197
                     /locus_tag="IWK70_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9615412.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSART"
BASE COUNT          455 a          154 c          244 g          344 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggac
//
LOCUS       DACDTO010000211         1193 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDTO010000211 DACDTO010000000
VERSION     DACDTO010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.221"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWK70_002956"
     CDS             <1..1076
                     /locus_tag="IWK70_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9615413.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          331 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tct
//
LOCUS       DACDTO010000212         1172 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDTO010000212 DACDTO010000000
VERSION     DACDTO010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.222"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..341)
                     /locus_tag="IWK70_002957"
     CDS             complement(<1..341)
                     /locus_tag="IWK70_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615414.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIER"
     gene            complement(358..>1172)
                     /locus_tag="IWK70_002958"
     CDS             complement(358..>1172)
                     /locus_tag="IWK70_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9615415.1"
                     /translation="ILVHYFFTGKIEDLFHEPLIEYTENLSKTIEQIKIDNLLSQNYD
                     PEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIRSEKNYSIIVFEKL
                     AQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYYTKEIGKTKIEHLS
                     PLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHITIYNYISKRKISTA
                     KIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLVDNFNNIS"
BASE COUNT          404 a          200 c          128 g          440 t
ORIGIN      
        1 tctctttcaa ttaactgatc caatttttga taatccataa attcatcatg tatcaaaaag
       61 aataaataac ttgatatttt agcatctagc ttaatcagat taaataatgt ttgtttaaaa
      121 ttgctaccat taatatgact gatagattct ctagcttctt cacataaact ttttacttta
      181 ttaattacta ctttgtcata ttcatttaac acactattat taccattact tagacgctca
      241 gattctacga aacaactcaa atcagaaatt tcccaaaaaa attttttgtt aaaatccaat
      301 ttcctcaact cctccaaact acgtaaatga tatagtctca taataaaata aaacctctca
      361 gctaatgttg ttgaaattgt ccactaaacc taattgatat tgcttaggag atacacctgc
      421 gtactttttg aatactctcg aaaagtgtga agaatctgaa aatcctaaac ctaaagaaac
      481 ttcactgata gtatggtttg atttcaacat tattttagcc gtagaaattt ttcgcttaga
      541 gatataatta taaatcgtaa tatgcatttc attcttaaag ctttctctta atttagtttc
      601 acttacagaa aaataattag ctatttcctt tacagtaatt cttttataga tatttaagcc
      661 gatgaattga acgacagaag aaattaaggg agaaagatgc tctatcttag tttttccgat
      721 ttccttagta taataaacaa tcccagaatc cctcactttt aagacctcag gtaacgatac
      781 agctaattcg ttttcttgta tcaaagcatc tctactttga tacgcttcta ttatatccat
      841 tcctaatgtt atagacgttt gtgctaactt ttcaaaaaca atgatagaat aatttttttc
      901 agaccgaatc gtatctccac taacacttgg aattattcca ttactcagat taaaaaccat
      961 gttttctaaa tttcgaatat ctccattttt aacatattcc agtattttat tttcataaag
     1021 aaacagatat atttctggat cgtaattttg tgataacaga ttatctattt tgatttgctc
     1081 gatagtttta cttagatttt ctgtatattc aattaacggt tcatggaata gatcttctat
     1141 ttttccagta aaaaaataat gaactaaaat ca
//
LOCUS       DACDTO010000213         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDTO010000213 DACDTO010000000
VERSION     DACDTO010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.223"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWK70_002959"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWK70_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9615416.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDTO010000214         1116 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDTO010000214 DACDTO010000000
VERSION     DACDTO010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.224"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="IWK70_002960"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="IWK70_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9615417.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          159 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaat
//
LOCUS       DACDTO010000215         1113 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDTO010000215 DACDTO010000000
VERSION     DACDTO010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.225"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..279)
                     /locus_tag="IWK70_002961"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IWK70_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IWK70_002962"
     CDS             complement(545..739)
                     /locus_tag="IWK70_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615418.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
BASE COUNT          390 a          187 c          159 g          377 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta ttacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ata
//
LOCUS       DACDTO010000216         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDTO010000216 DACDTO010000000
VERSION     DACDTO010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.226"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(37..993)
                     /locus_tag="IWK70_002963"
     CDS             complement(37..993)
                     /locus_tag="IWK70_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615419.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DACDTO010000217         1051 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDTO010000217 DACDTO010000000
VERSION     DACDTO010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.227"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IWK70_002964"
     CDS             6..980
                     /locus_tag="IWK70_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9615420.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          377 a          132 c          182 g          360 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaacatttt t
//
LOCUS       DACDTO010000218         1010 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDTO010000218 DACDTO010000000
VERSION     DACDTO010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.228"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWK70_002965"
     CDS             <1..848
                     /locus_tag="IWK70_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9615421.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          329 a          195 c          202 g          284 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt
//
LOCUS       DACDTO010000219          997 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDTO010000219 DACDTO010000000
VERSION     DACDTO010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.229"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(26..913)
                     /locus_tag="IWK70_002966"
     CDS             complement(26..913)
                     /locus_tag="IWK70_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9615422.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DACDTO010000220          994 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDTO010000220 DACDTO010000000
VERSION     DACDTO010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.230"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>994)
                     /locus_tag="IWK70_002967"
     rRNA            complement(<1..>994)
                     /locus_tag="IWK70_002967"
                     /product="16S ribosomal RNA"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DACDTO010000221          988 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDTO010000221 DACDTO010000000
VERSION     DACDTO010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.231"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IWK70_002968"
     CDS             <1..704
                     /locus_tag="IWK70_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9615423.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          335 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataataa
//
LOCUS       DACDTO010000222          959 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDTO010000222 DACDTO010000000
VERSION     DACDTO010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.232"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(107..511)
                     /locus_tag="IWK70_002969"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="IWK70_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>959)
                     /locus_tag="IWK70_002970"
     CDS             complement(570..>959)
                     /locus_tag="IWK70_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9615424.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          170 c          159 g          343 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt tcggtcttt
//
LOCUS       DACDTO010000223          936 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDTO010000223 DACDTO010000000
VERSION     DACDTO010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.233"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            47..>936
                     /locus_tag="IWK70_002971"
     rRNA            47..>936
                     /locus_tag="IWK70_002971"
                     /product="23S ribosomal RNA"
BASE COUNT          288 a          185 c          273 g          190 t
ORIGIN      
        1 ctaacttcta tcgctagaag aaggatcaaa acccaaccgt aaggttgata aggttaagtg
       61 aataagggcg cacggtggat gccttggcac taggagccga tgaaggacgg gactaacacc
      121 gatatgcttt ggggagctgt acgtaagcta tgatccagag atttccgaat gggggaaccc
      181 aacatcttta ataggatgtt acgattgtgt gaatacatag cacattcgag gtagacgcag
      241 agaactgaaa catctaagta cctgcaggaa gagaaagaaa attcgattcc ctgagtagcg
      301 gcgagcgaaa cgggaaaagc ccaaaccagc aagcttgctt gttggggttg taggactcca
      361 atatggtagt tctttcagat agtcgaatga cttggaaaag tcagtcaaag agggtaaaaa
      421 ccccgtagac gaaatgtgga agacacctag gaggatcctg agtacggcgg aacacgagaa
      481 attccgtcgg aatccgggag gaccatctcc caaggctaaa tactccctag tgaccgatag
      541 tgaaccagta ccgtgaggga aaggtgaaaa gcaccccgga aggggagtga aatagaacct
      601 gaaaccgtgt gcctacaaca agtcaaagcc cgttaatggg tgatggcgtg ccttttgtag
      661 aatgaaccgg cgagttacga ttgcatgcga ggttaagttg aagagacgga gccgcagcga
      721 aagcgagtct gaatagggcg tttgagtatg tagtcgtaga cccgaaacca tgtgatctac
      781 ccatgtccag gttgaaggtg cggtaaaacg cactggagga ccgaacccac gtacgttgaa
      841 aagtgcgggg atgaggtgtg ggtagcggag aaattccaaa cgaacttgga gatagctggt
      901 tctctccgaa atagctttag ggctagcctc ggaatt
//
LOCUS       DACDTO010000224          919 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDTO010000224 DACDTO010000000
VERSION     DACDTO010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.234"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>919
                     /locus_tag="IWK70_002972"
     CDS             <1..>919
                     /locus_tag="IWK70_002972"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9615425.1"
                     /translation="NVFKKQNKLLTLGSCLAQVIFQLWIGGIFLIDVILLAKGTISIT
                     NFVIVFMYANMVPTLWTTLISIIWKWQDYSVSLNRYYEYYSNNNKVAGTKKINKINSI
                     TGQSLNFSYNKRKVLSNISFSTEMNKLFMISGENGTGKTTLMKLFTKQLPVSNNQLFV
                     NGIDINMIDNESLRRLISIATQIPYIINSSVKENILMGTKNYDKKKFSKLIELLGISK
                     FMTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDEPFRNLDNETRDNL
                     RDYILNTKKEVCYIIITHDHILDNFLDSN"
BASE COUNT          380 a           99 c          140 g          300 t
ORIGIN      
        1 ttaatgtttt caaaaaacaa aataaattac taacactggg aagctgtttg gcacaggtta
       61 ttttccaatt atggattgga ggaatatttc ttattgatgt tattttatta gctaaaggaa
      121 caataagtat tactaatttt gtaatagtat ttatgtacgc taacatggtc cctactttat
      181 ggactacttt aattagtatt atttggaaat ggcaagacta ttcagtgtca ttaaatagat
      241 attacgaata ttattcaaat aacaacaaag tggctggcac aaaaaaaata aataaaataa
      301 attctattac tggtcaatca ttaaattttt catataacaa aagaaaggta ttaagcaata
      361 taagtttttc aactgaaatg aataaactat tcatgatatc aggagaaaat ggaacaggga
      421 aaacaacact gatgaaatta tttactaaac agttgccggt tagtaataat caattattcg
      481 tcaacgggat agatattaat atgattgata acgaatcatt aagaaggtta atttcaatcg
      541 caacacaaat accatatata attaatagct ccgtgaagga gaatatttta atggggacta
      601 aaaactatga taaaaaaaaa ttcagtaaat taatagaact attgggaata agtaaattta
      661 tgacgaaatt aggtattact ttggacgaca atatttatga tgaaaaaata ttattatctg
      721 gaggagaaaa acaaaaaata agtttagcta gggcgcttat taaatcagct tcagttatta
      781 tattagatga accatttaga aatttagata atgaaactcg cgataatttg agggattata
      841 tactaaatac aaaaaaagaa gtttgttata taattataac tcatgatcac attctagata
      901 attttttgga ttcaaatga
//
LOCUS       DACDTO010000225          894 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDTO010000225 DACDTO010000000
VERSION     DACDTO010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.235"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            93..422
                     /locus_tag="IWK70_002973"
     CDS             93..422
                     /locus_tag="IWK70_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9615426.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="IWK70_002974"
     CDS             412..699
                     /locus_tag="IWK70_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9615427.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          327 a          109 c          161 g          297 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
      721 aaggacggct tctttttatc gaaaatctga ttcgtctaca tgactaaagt cgcgagtatt
      781 ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata aatcaatata
      841 tttcagcttt tccttattta tgtactacct tttacttatt aattataagg tagt
//
LOCUS       DACDTO010000226          890 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDTO010000226 DACDTO010000000
VERSION     DACDTO010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.236"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..888
                     /locus_tag="IWK70_002975"
     CDS             <1..888
                     /locus_tag="IWK70_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP9615428.1"
                     /translation="RNLDSRTRFEQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYY
                     RVKKLDKPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMG
                     LTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPIL
                     DLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLK
                     ENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKR
                     IKLKLNGLSPVEYRKQSIK"
BASE COUNT          387 a          116 c          144 g          243 t
ORIGIN      
        1 agaaaccttg attcaagaac aagatttgag caagaagaaa tcaaggttaa aacaatcctt
       61 cagctcaaaa aagaatttaa attgaatcta ttactttcaa ttgcacaatt agcgaagtca
      121 acttattatt acagggtaaa aaaattagat aagccagata aatatagcaa gataaagcaa
      181 gaaattacag cgattgtaaa agaatcaaga aactcttatg gttatcgtag agtcacttta
      241 gcgttaaaga tgaaaggata tacaatcaat cataaaacag ttagaaaatt aatgtcccaa
      301 atgggactta cctgtcaaat ccgaataaag cgatataaat cttataaagg aacagtagga
      361 aaaattgcca agaatgtgtt aaaacgaaat ttttcagtag atacacccaa taaaaaatgg
      421 gtgacagacg tcactgaatt caagataaaa ggaagaaaaa tctatctatc tcctatactt
      481 gatttattta acggagaaat aattagttat agtatatcaa ctagtccaac atataaactg
      541 attgaagaaa tgcttcaaca agcaattaaa aaaaagggaa ctgagggttc actgattcta
      601 cattcagatc aaggatggca gtatcagatg ccacaatatc aaaaaaaatt aaaagagaat
      661 aacattattc aaagcatgtc tagaaaagga aattgcttag ataattctgt aatagaaaat
      721 ttttttggtg tgcttaaatc agagtttttt tatcgagaaa aattccgatc aattgagata
      781 tttcaaagta aattaaatga gtatattagg tggtataata acaaaaggat aaaactaaag
      841 ttaaatggac tatctccagt agaatatcgg aaacagtcca ttaaatagtt
//
LOCUS       DACDTO010000227          870 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDTO010000227 DACDTO010000000
VERSION     DACDTO010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..870
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.238"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>870
                     /locus_tag="IWK70_002976"
     CDS             <1..>870
                     /locus_tag="IWK70_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9615429.1"
                     /translation="SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHY
                     PKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIR
                     AYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDS
                     IEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEY
                     SYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISEC
                     TIDNKQNVLEEYI"
BASE COUNT          368 a           76 c          142 g          284 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat
//
LOCUS       DACDTO010000228          787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDTO010000228 DACDTO010000000
VERSION     DACDTO010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.239"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          278 a          141 c          107 g          261 t
ORIGIN      
        1 aaccgggggc tttattcctg cagtccggct acgccttcct gcaatagaca aaactccaga
       61 tcgggtaatt cgcttgtggc gaaacccgac ctggagtttg tctctgtatt ttgaacccta
      121 aaaattaatc aaacagactg atattctcat aatcagcgcg aaataagcca ttaagtacag
      181 ttttcctacc tatttttgat aacccgctgg ttagttaata ttccttttcc gagagagata
      241 aatgattggt taaatctata tacatctcaa cctttaaaaa tgttttcgaa atacttcatc
      301 agtacatgtc atacaaatta ccagtgcatt ctcaaattct agttctttaa ttgatttcag
      361 ttgaggagca gttacttttg gcggaaattt ccatttttgt ccaaacctct tcccccgatg
      421 aaatttcttt gttgcaatta ttgcaatgaa aaatttttgt ttcaaaaata tacataatta
      481 cttcctccaa taaaaactat ctacctctca aattattttt ctctaataag aaaaacagaa
      541 aaggtaataa gatacaacag tgatgattcc taataaagaa aagactacaa taatattttt
      601 ctaacttatc gccttcaaag aaatagtttc ttatttttca ctaaaataaa aaatttattg
      661 caaaaaaaat agaagtgaca aaattatcaa tcatttcaag gtaatataac tacctgagtt
      721 tattatcgat taaaatgcta actacatttg caagagaagc gagagttaaa ggtaaatata
      781 gaatttc
//
LOCUS       DACDTO010000229          768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDTO010000229 DACDTO010000000
VERSION     DACDTO010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.240"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(109..>768)
                     /locus_tag="IWK70_002977"
     CDS             complement(109..>768)
                     /locus_tag="IWK70_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP9615430.1"
                     /translation="RIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQ
                     PLEAIVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLT
                     DVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQY
                     QFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEA
                     A"
BASE COUNT          213 a          176 c          147 g          232 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatggcttc caatggctgt tcttgtgtaa aggtccgatc
      661 taatacattc gctgttttcg cttcattaca agctgttcgt tgccctttaa aatgagcgat
      721 cgtataggta gatgtcaatc cgcggcgttt catgattcga ccgattcg
//
LOCUS       DACDTO010000230          768 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDTO010000230 DACDTO010000000
VERSION     DACDTO010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.241"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(44..502)
                     /locus_tag="IWK70_002978"
     CDS             complement(44..502)
                     /locus_tag="IWK70_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9615431.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKLTRV"
BASE COUNT          229 a          133 c          100 g          306 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttatact ctcgtcaact
       61 ttctcttttc aaaaaaaatt tgatattttt ttagatttct ttttagtctt tgtaatactt
      121 tataagccac agaataactc acgtgatgct gtttagcaaa attttctact gagatatatt
      181 tttcctgaaa gaaagccaat accattttca tagataaaga atccgttata taacgagata
      241 acagagcttt gctagaacat tttccattaa tttccagaca tatatccaat tcactgacgg
      301 ttactcgcaa atttttctct agatcgaact tttgtaaatc atgtttcagc ttatcgatcg
      361 tttttttcaa gagaaatgtc gatagcgaca actcttcttg aacattcgat attgactctg
      421 ataaatttga gcttgcttct aaatatttaa gtaatgaata acttctgtga tcttctttgc
      481 ttaaaaaaaa ctcataattc attagtcatc agctctttct acgtatatta ctttccatgc
      541 attcaccaac tagctgggga taaaactgat ttcctggatc cgagtcactg ttgtcagcga
      601 ttcttgtttg tataatatac atcgtcctgt ctttttttta acattttgga gagcgtttta
      661 tttggccttt ctcgcgatca aacctgttat tagaattata tcatttacta gtatgcattt
      721 ttattaataa aatggtatat tttatattgt tataaaaaga gcccaacc
//
LOCUS       DACDTO010000231          717 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDTO010000231 DACDTO010000000
VERSION     DACDTO010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.242"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            260..541
                     /locus_tag="IWK70_002979"
                     /pseudo
     CDS             260..541
                     /locus_tag="IWK70_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            615..>717
                     /locus_tag="IWK70_002980"
     CDS             615..>717
                     /locus_tag="IWK70_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9615432.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT          258 a           91 c          125 g          243 t
ORIGIN      
        1 aaagtttttg atcctttatt ttcaagtctg atttggcagc ttttcggcag ctcatttatt
       61 tatggtcaga aatagatgct gaaattttaa tctattagca aatagtccag aacgtatgaa
      121 gaggagtcgg atatcatttg tcattaacac cataaatatt tataaaattc gtgttcacct
      181 acaattaaat ttaatatata atattcgttg taagggctta taattttttg ttaatgaaca
      241 agataggaag aaacatttaa tggacagttt tcagagattg tcactagtaa atcaatttga
      301 gttattggct aaatttgagg accaaaacag atcttattat gaaagaaaaa tcgaaatatt
      361 gagagaaggt tacgagtatc attatgatga tgaaatttgg agtgatcttt ctgagccatt
      421 tccgaaagaa gattctcgat ttgtattaga tgtattgaac atgtatcgag atatcaattt
      481 tagtaaatca aagctaaact tagatgaaaa ccaaattact aattcatact atactcattt
      541 cggttctgtt gcaaagtttt ccaaaaaatc tattttagtg taaaattgag aaaaaagaca
      601 gagaggacag agtaatgaat cattttaaag gcaaacaatt caaaaaagac gtcattattg
      661 tcgctgttgg ttactacctg cgttacaatc taagctatcg tgaagttcag gaattgt
//
LOCUS       DACDTO010000232          707 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDTO010000232 DACDTO010000000
VERSION     DACDTO010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.243"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWK70_002981"
     CDS             132..344
                     /locus_tag="IWK70_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615433.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>707
                     /locus_tag="IWK70_002982"
     CDS             506..>707
                     /locus_tag="IWK70_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615434.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNM"
BASE COUNT          253 a          104 c          123 g          227 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgt
//
LOCUS       DACDTO010000233          690 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDTO010000233 DACDTO010000000
VERSION     DACDTO010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.244"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            124..327
                     /locus_tag="IWK70_002983"
     CDS             124..327
                     /locus_tag="IWK70_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9615435.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            327..677
                     /locus_tag="IWK70_002984"
     CDS             327..677
                     /locus_tag="IWK70_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9615436.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          287 a           70 c          115 g          218 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag
      421 atattactga tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa
      481 ttcttgtaaa taaattagtt aattatattt acatcgtggg ttttgataat aaaatacgct
      541 ttattgataa tgatgaaaaa ttattaatag aattagggga tatttctaaa aaagctggaa
      601 ttaatagtaa atacaaagca aactttacag ataaatcgca attttatagt tattcagaaa
      661 caatgcctca acgataaaat atagtatact
//
LOCUS       DACDTO010000234          677 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDTO010000234 DACDTO010000000
VERSION     DACDTO010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.245"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            252..650
                     /gene="spxA"
                     /locus_tag="IWK70_002985"
     CDS             252..650
                     /gene="spxA"
                     /locus_tag="IWK70_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9615437.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          239 a           99 c          127 g          212 t
ORIGIN      
        1 gttttatacg ctgtttcata tgtttagaaa atttgaatta gatttgaaaa ctctaatttt
       61 taaatgataa aaaaagcgag aatgctggtt ttactgtata ttaaccttag ataactttca
      121 tttttttaaa aaaattatga aaaaaaggta gctatttaaa agttgattat gtataataga
      181 aacagatggg agattattat gatcttctgt tacaaaaaag tgattcttat cacgaaaagg
      241 agtggtgcac gttgttaaca ttatatactt ccccaagttg tacttcctgc agaaaggctc
      301 gtgcgtggtt gcaagaacat cagattccat ttgttgaacg taatattttt tcagaaccat
      361 tgaacagttc tgaattaaaa gcgattttac aaatgacaga agacggaaca gaagaaatca
      421 tctcgacacg ttccaaagta ttccaaaaac tgaacatgga tttagatgat ctgccgttac
      481 aagaattatt ggaacttgtg caaaacaatc ctggattgct tcgccgtcca atcatgattg
      541 atgataaacg tctgcaagtt ggttttaatg aagatgaaat tcgtcggttc ctaccgcgcg
      601 atgtacgaca attagaatta cgtcaagcac aattgatggc tggattatag aaggataata
      661 cgaaacttgg aaaagac
//
LOCUS       DACDTO010000235          670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDTO010000235 DACDTO010000000
VERSION     DACDTO010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.246"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            320..>670
                     /locus_tag="IWK70_002986"
     CDS             320..>670
                     /locus_tag="IWK70_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HAP9615438.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNK"
BASE COUNT          250 a           75 c          107 g          238 t
ORIGIN      
        1 gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt gaactaatag
       61 agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat agttggttat
      121 tataaaatag taatatagct gatttggatt caattcatat ttatttataa atcaaaaata
      181 cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta cttacttaaa
      241 ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc agtgaattta
      301 taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt tagacggtac
      361 tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga aacatttacc
      421 ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta ataatattct
      481 caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg gatttgatgg
      541 agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa ttgattctgt
      601 cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat ctcagaatta
      661 taaaaataaa
//
LOCUS       DACDTO010000236          631 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDTO010000236 DACDTO010000000
VERSION     DACDTO010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.247"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            114..>631
                     /locus_tag="IWK70_002987"
     CDS             114..>631
                     /locus_tag="IWK70_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9615439.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMS"
BASE COUNT          246 a           61 c          142 g          182 t
ORIGIN      
        1 tgtttaagtt gacatggaaa caatctttta aaaagcaaaa agatgataaa gttacttttt
       61 atgggatgtt ggtagaaaac aaattattat aaatattaga ggtataaaaa gttatgaaac
      121 acaaggtaag tgtgatagta ccaatttaca atgttgaaaa atatttaaga aaatgtgtgg
      181 atagtttatt gagtcagaca ttaaaagaga tagaaatcgt attagttgat gatggttcgc
      241 cagatgcttc gggggaaata gcagatgagt atcaaaaaaa atattctaat gtaaaaacaa
      301 tacatagaga aaatgggggg ttaggaccgg caagaaatac aggaattgag aatgcgacag
      361 gagaatatgt ggcttttctt gattcagatg attgggtaga aagtgatatg tatgaaaaat
      421 tatacttagt tgcgtccaaa gaaaatgctg atatagtagt tagtggacat tgtgattttg
      481 cgaatgacaa agctatggtg aaaaagcaac atcctttgag tgggaatgta tatgatacgc
      541 ctaatagtat taaaaatgta cgaaaaaatt tatttggaca tagtccaaaa gatcaagaag
      601 tagaggcctt cccgatgtca gtatgtatgt c
//
LOCUS       DACDTO010000237          606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDTO010000237 DACDTO010000000
VERSION     DACDTO010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.248"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          190 a           97 c           93 g          226 t
ORIGIN      
        1 ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt cataaaaaac
       61 gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag agagacaatt
      121 tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt acaaacgtct
      181 tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc cttatctaga
      241 ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt tattttcgtt
      301 ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc ggtattgcat
      361 atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta gttcttgaaa
      421 gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt tgatgcaatt
      481 aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct attttctttt
      541 aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat ataaatcata
      601 tgctca
//
LOCUS       DACDTO010000238          606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDTO010000238 DACDTO010000000
VERSION     DACDTO010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.249"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..499
                     /locus_tag="IWK70_002988"
     CDS             <1..499
                     /locus_tag="IWK70_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAP9615440.1"
                     /translation="YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIK
                     QPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEF
                     VYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCAN
                     ASEAV"
BASE COUNT          184 a          111 c          131 g          180 t
ORIGIN      
        1 ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc attttggatc tgttcaatcg
       61 agaaattctc ggctattctt gtggagaaca taaagatgcc gttctagtaa aaaaagcatt
      121 tagccgtatc aaacaacctc tgacagaggt tgagattttt catactgatc gtggaaaaga
      181 gtttgataac caagctattg atgaattatt aacaactttt gacatcaatc gatcattgag
      241 tcataaaggc tgtccttttg ataatgccgt agctgaatca acttataagt cgttgaaagt
      301 agaatttgtc tatcaataca catttgaaac cttacaacaa ttggatttgg agttatttga
      361 ctatgtcaat tggtggaacc accttcggtt gcacggtaca cttggctacg agacaccggt
      421 tggttaccgt aaccagagat tggcgcagcg aatccttgat aatgagctcg gatgtgctaa
      481 cgctagcgag gcagtctaac tttaactgtt agctcctgcc gaagatcgtc acatccgagg
      541 aggctcattg tcaaggacaa tcggaatagc atacggaaag aagttctgca ccttataaaa
      601 tttgtc
//
LOCUS       DACDTO010000239          587 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDTO010000239 DACDTO010000000
VERSION     DACDTO010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.250"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..193
                     /locus_tag="IWK70_002989"
     CDS             <1..193
                     /locus_tag="IWK70_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615441.1"
                     /translation="LLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVIS
                     IVFWYYLGSISYLYLKKEN"
     gene            195..>587
                     /locus_tag="IWK70_002990"
     CDS             195..>587
                     /locus_tag="IWK70_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615442.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKS"
BASE COUNT          198 a           65 c           95 g          229 t
ORIGIN      
        1 tttactcaaa acatttttta gaagtaaagt gaattatgcg tttcttggta ttgttttaat
       61 ttttatacta tctattttta tagatgaaat ggtggaggct catgtacttt tcaataagcg
      121 cgatgtcatt tcaattgttt tctggtatta tttgggaagc atttcttact tgtatttaaa
      181 aaaggagaat taatatgaag acagctttca ttgcttggac tcctttacat ttgattaata
      241 tattaaatgt ttatcaaaat ttttaccgag aatcaaaagg agatttattt atttattctg
      301 aatttgaaaa cgctagaaga tatagtgagc gtataaaaga attagggcta tttaatgatg
      361 ttgtttttgt gaatcaccgg aaaatcggtt cttcctttaa acgtaaaatt aacttgttaa
      421 ctaaccataa tcaatttttc tcggatgatt tatccgttta tgatgaaatt tttatacagg
      481 gggaaaatta tttcacaaaa atacttttct ccaatgttta caaaaaaaat ccagatgtaa
      541 aactaaatta tatagaagat ggtttgggta cttatttgaa gaagagt
//
LOCUS       DACDTO010000240          526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDTO010000240 DACDTO010000000
VERSION     DACDTO010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.251"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWK70_002991"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="IWK70_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9615443.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DACDTO010000241          517 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDTO010000241 DACDTO010000000
VERSION     DACDTO010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.252"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            57..>517
                     /locus_tag="IWK70_002992"
     CDS             57..>517
                     /locus_tag="IWK70_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9615444.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DACDTO010000242          509 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDTO010000242 DACDTO010000000
VERSION     DACDTO010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.253"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(1..177)
                     /locus_tag="IWK70_002993"
                     /pseudo
     CDS             complement(1..177)
                     /locus_tag="IWK70_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
BASE COUNT          175 a           81 c           60 g          193 t
ORIGIN      
        1 aattgctttc atattaggaa ttggatcttc acctggcata tattgcagat tagaagcatg
       61 taattttttc tcagaaacaa accactcatt ggaaaagaca tgaatcaaat catcacttac
      121 ttgtgtaaaa tagcgacgtt taatgacaaa gatatcttca ttgctatcta gtgtaccttc
      181 atttctttgt gcttaaattc aaagataatt ttctcaataa agatttttga ttccagtaag
      241 attcctttac tccttttaca acatagcgtt gtcctttgtt ttgaaacatc ttcttcccct
      301 ctttgccatt aaaaatagcc caattctcta taaagaagat taagcttcat tattataata
      361 taaagtttaa atttggttta ttacttactt tttcttattc tctagtaagc taatattgaa
      421 aaaaatgtaa tcagatcatt atttttatat gttgtaactt cctgataaga aaactaatta
      481 aaagaaagag gtaactacat aaactaatt
//
LOCUS       DACDTO010000243          505 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDTO010000243 DACDTO010000000
VERSION     DACDTO010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.254"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          226 a           70 c           81 g          128 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actct
//
LOCUS       DACDTO010000244          497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDTO010000244 DACDTO010000000
VERSION     DACDTO010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.255"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>497
                     /locus_tag="IWK70_002994"
     CDS             <1..>497
                     /locus_tag="IWK70_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9615445.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFT"
BASE COUNT          186 a           55 c           81 g          175 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacga
//
LOCUS       DACDTO010000245          497 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDTO010000245 DACDTO010000000
VERSION     DACDTO010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.256"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>497)
                     /locus_tag="IWK70_002995"
     CDS             complement(<1..>497)
                     /locus_tag="IWK70_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9615446.1"
                     /translation="CWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIP
                     KWVEEKKNKGIITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRR
                     PDYAHASVASGYFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLA
                     QRINH"
BASE COUNT          162 a          103 c           55 g          177 t
ORIGIN      
        1 catgatttat tctctgtgct aaaacaatca tataatctat catcaaataa tccaccatca
       61 tatcattatt tttccaataa tttaaaaaaa agtctctaat tatagcaaac atccatctag
      121 actcttgatt acatgctaat gaataacccg caaaatatcc tgatgctacg gatgcgtgag
      181 cataatcagg cctcctaata gaccaaatat cgatatcaaa atactcatct ggtattggtt
      241 ttgtacaata aaatgtggcg tctaaccaca aacctccatg ttttgctagc aaagataatc
      301 ttaataaatc agaataattt gttctcgtaa tgattccttt gtttttcttt tcttccaccc
      361 attttggtat atcaacatat tgtctataat tatcttctgt taaaattata acagtatgtt
      421 tccctgcatt tcgttttatt gattttacac aagcttttac aatttccggc gattgatcca
      481 atccctgcca ccaacaa
//
LOCUS       DACDTO010000246          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDTO010000246 DACDTO010000000
VERSION     DACDTO010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.257"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(61..258)
                     /locus_tag="IWK70_002996"
     CDS             complement(61..258)
                     /locus_tag="IWK70_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9615447.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DACDTO010000247          477 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDTO010000247 DACDTO010000000
VERSION     DACDTO010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.258"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>477)
                     /locus_tag="IWK70_002997"
     CDS             complement(<1..>477)
                     /locus_tag="IWK70_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9615448.1"
                     /translation="SSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFK
                     IFRAIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFK
                     IGMFAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPS"
BASE COUNT          159 a           65 c           68 g          185 t
ORIGIN      
        1 ggaaggatca ataatatgaa tatttgctaa tttcgaagtt tgtaaagtag tttcttcaat
       61 atttttcaat tcaagtggta aatctggtcc tccaataaca tttttatttt tgaacgagga
      121 attaaaaaaa agtgtatgaa aaaatttttc atctggggca aacataccta ttttaaaatt
      181 caggtagtct tgttgatttt gttcatacat ttgtattaat tctgttaggc attctttcga
      241 caaagcccac cattgagaac catgatacaa atcaaattta tggtgtctaa tttttggggg
      301 aagtttaatc atcctcagac aacgattaat tatagctcta aaaattttaa atgcagttgt
      361 gttattccta ttaattctag gataatcatg tttttgatat acatctatat gtttataata
      421 aaattcttta tgtttaattt gatctagatc tattccacga ataaactcca ctgaact
//
LOCUS       DACDTO010000248          466 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDTO010000248 DACDTO010000000
VERSION     DACDTO010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.259"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>466
                     /locus_tag="IWK70_002998"
     CDS             <1..>466
                     /locus_tag="IWK70_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9615449.1"
                     /translation="FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTE
                     DNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKE
                     VKLKERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILSTIITPGNIH"
BASE COUNT          168 a           84 c           95 g          119 t
ORIGIN      
        1 ccttcgagga gaaaatcccc catttctcaa cttttggaaa gaattatgtt cgccgattta
       61 gagaaacgac ggtgtttgag gatatttttg cctatatttt agaacaagca gttaaagcag
      121 gttttgtaac agaggataat ttgtatctgg attctaccca catcaaagct aacgccaata
      181 aacataagtt tacgaaagaa atgacccatg atgaagcgaa agcctatcag gatgaattag
      241 aagatgagat caatcgtcaa cgaatagagg ctggaaaacg cccttttact ttggacttag
      301 aaaaggaagt gaagctgaaa gaaagaaaaa taagtaaagc tgatccggaa agcggctact
      361 acgtcaaagg cgaacgagaa aaacaatttg cttattctgc ccatacttca tgcgacgaca
      421 atggatttat tcttagcaca atcatcactc ctggaaatat tcatga
//
LOCUS       DACDTO010000249          415 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDTO010000249 DACDTO010000000
VERSION     DACDTO010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.261"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..>415)
                     /locus_tag="IWK70_002999"
     CDS             complement(<1..>415)
                     /locus_tag="IWK70_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615450.1"
                     /translation="ESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNLFSF
                     TSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINEVSI
                     LEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSK"
BASE COUNT          146 a           59 c           53 g          157 t
ORIGIN      
        1 ctttagatag taccgcttct ttaaaaaaat gtgaatcttt taaatagggg attagtgatt
       61 catcttctgt tgaatttagc aaatgaatca ctagcttctt tacttctaaa attgaaactt
      121 cgttaatatt ttctaatact gttgtcaaaa tagtcgtagt atatgcaggc tcacatatga
      181 ggtaatttac tatactgtag gataagtttt gaaatggatt aacaattata gaaaccattc
      241 ctacaattct ctctttatgt cttaaaaagt aaaattcact cgtaaacgaa aataaattat
      301 atctaaggac aacttctaat tctttactat ttccttgatg ataaatatct tcatgaaaat
      361 acatactttc aggatgatct gtgataaaat tataaatttc ttcataatgt gattc
//
LOCUS       DACDTO010000250          409 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDTO010000250 DACDTO010000000
VERSION     DACDTO010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.262"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..379
                     /locus_tag="IWK70_003000"
     CDS             <1..379
                     /locus_tag="IWK70_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9615451.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     RKEGTLFGFSVCTEIKVLLGIPA"
BASE COUNT          139 a           79 c           81 g          110 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt
      361 attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattctt
//
LOCUS       DACDTO010000251          404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDTO010000251 DACDTO010000000
VERSION     DACDTO010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.264"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>404
                     /locus_tag="IWK70_003001"
     CDS             <1..>404
                     /locus_tag="IWK70_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9615452.1"
                     /translation="PEDKLPVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDK
                     SDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVV
                     VTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          166 a           60 c           92 g           86 t
ORIGIN      
        1 ccagaagata aacttccagt gttgaaagca aaagatggtt atacagatgc gaaatggcca
       61 gaggaagcaa ctcaaccaat tacagcagat gatacagaat ttgtatcaag tgcaacaaaa
      121 ctggatgata aatctgacgc tgacaaatat aatcctgaag gtcaaaaagt gactacagaa
      181 ttgaataagg aaccagacgc atttgaggga attaagaaca aagaagattt accaaaagat
      241 actaagtata cttggaaaga aaaagtagat gttagcgcag ctggaaataa aaaagggact
      301 gttgtagtga catatccaga tggatcatct gatgaagttg aagtagatgt tacagtgaca
      361 gacaatcgtt ctgacgccga taaatatgag ccaacagtag aagg
//
LOCUS       DACDTO010000252          375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDTO010000252 DACDTO010000000
VERSION     DACDTO010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.265"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWK70_003002"
     CDS             complement(<1..132)
                     /locus_tag="IWK70_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9615453.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DACDTO010000253          369 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDTO010000253 DACDTO010000000
VERSION     DACDTO010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.267"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          143 a           57 c           54 g          115 t
ORIGIN      
        1 tgcaaaatat tgtaaattta aagcctttgt agaaaaattt actgaaagac aaactgataa
       61 gattattcag tatcatatta cctatactga ttaaaatttt ttgaaacggg atactaaata
      121 aaaaatttat ttataacaga actaattatt taatgcggat gcgccaatac ttggtcctgc
      181 aagagctatt tattttgatc gccgtttgta taattaagcg agacaaataa aaaagccatt
      241 tatgttacac tcaaattgta atacccgcta aagcatacaa ggagagtgaa cataaatgac
      301 ttatacccat cttacaccaa acgagcttgt aatgatagaa gcatattttc atcaagaaac
      361 tccggttgt
//
LOCUS       DACDTO010000254          363 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDTO010000254 DACDTO010000000
VERSION     DACDTO010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.268"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..>363
                     /locus_tag="IWK70_003003"
     CDS             <1..>363
                     /locus_tag="IWK70_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9615454.1"
                     /translation="FRSVEIFLRGAMGELLAMSILPLILLGFIKLYDSKKESWVMLAI
                     SMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIKLIKAAIVTLLLSLAFIG
                     PMIEQTLYQDLNRPYVDI"
BASE COUNT          113 a           50 c           62 g          138 t
ORIGIN      
        1 cttttaggag tgtagaaata tttcttcgag gggcaatggg ggaattactc gcaatgtcaa
       61 tacttcctct tattttactc gggtttataa agctttacga ctcaaaaaaa gaatcttggg
      121 ttatgttggc gattagcatg actttattaa tttacactca tgttctttct gtagctatga
      181 ctatggtaat gattataatt gctttaatta ttcaatttta tcggaaaaag aaaatagaaa
      241 tcttcttaat tattaaatta ataaaagctg ctatagttac tttacttctt tcactagcgt
      301 ttataggtcc tatgattgag cagacattgt atcaagatct gaatagacct tatgtggata
      361 tat
//
LOCUS       DACDTO010000255          353 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDTO010000255 DACDTO010000000
VERSION     DACDTO010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.270"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(<1..321)
                     /locus_tag="IWK70_003004"
     CDS             complement(<1..321)
                     /locus_tag="IWK70_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9615455.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT           97 a           56 c           66 g          134 t
ORIGIN      
        1 taaatggata atagtggttt ccaatgtatc gtttgttctt ttgtaggctg gtaaagtttg
       61 ttgtttaaac tcaccattac gatctctagg tatttccaat gttaattcac catattcggt
      121 tttgattgat cgaaagtaag aaccgtttct cgaattacct gaattaaaac cagtgcgatc
      181 atatttttcg taatctaaaa aagccgttaa ttcagtccgt aggagtgtgt ttatcgcttt
      241 ttctaagtgc gaacggaata attcatttaa atcgccttta gtgactagag tttgcacaat
      301 ttctgtagta aaatcattca tagggaagtc ctcttttctg tgaattggtt gtc
//
LOCUS       DACDTO010000256          336 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDTO010000256 DACDTO010000000
VERSION     DACDTO010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.denovo.272"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
BASE COUNT          135 a           51 c           47 g          103 t
ORIGIN      
        1 ctaggagtga gcatttttcg aaaaatacta aaaatttttc aaaataaaaa aaggcataca
       61 gacaataaac tgtctatacg cctcacagac tctattctat acatctaatt ttggctgtcc
      121 tgcaaaaaat atgtgttagc ctccatcgaa agatcaaact ttggaaaaca tcattataac
      181 tttgcttggt aagagctttg ctaaaacatg atatgacttg tatacaaaat gtggagtgta
      241 ttccaataaa taggaaggat tacaaaattt gaaaccaaat aagtaaaaaa tactgtcact
      301 ttgaattaat ttgaaatagt aaattctcat taaact
//
LOCUS       DACDTO010000257         6779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.114, whole genome shotgun sequence.
ACCESSION   DACDTO010000257 DACDTO010000000
VERSION     DACDTO010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.114"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..1947
                     /locus_tag="IWK70_003005"
     CDS             <1..1947
                     /locus_tag="IWK70_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9615456.1"
                     /translation="TEWGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILF
                     LAFGGVILTLVGVGATLSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAM
                     HILEGEGLLNDASGVTAFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQ
                     KIIHLIEKASAQDVTGYLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKIS
                     VFDAELSSLSHSTWTTIAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLI
                     SVMLFVIRFISISLFYVFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINN
                     MMFYQRSLLLFLTAGVILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKE
                     IKEIDRNTKEFLATEAVIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDE
                     SYRSGKLSSNGYRFYSRFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRR
                     YADRQTFISDEDIQEIRETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMN
                     SGNLISSWMIQQDSLFTKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESY
                     TMNKPSDNFSFRFAFRAKSKKRVQ"
     gene            2112..3998
                     /locus_tag="IWK70_003006"
     CDS             2112..3998
                     /locus_tag="IWK70_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9615457.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4010..4957
                     /locus_tag="IWK70_003007"
     CDS             4010..4957
                     /locus_tag="IWK70_003007"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9615458.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            4977..5504
                     /locus_tag="IWK70_003008"
     CDS             4977..5504
                     /locus_tag="IWK70_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9615459.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(5567..6220)
                     /locus_tag="IWK70_003009"
     CDS             complement(5567..6220)
                     /locus_tag="IWK70_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9615460.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            6352..>6779
                     /locus_tag="IWK70_003010"
     CDS             6352..>6779
                     /locus_tag="IWK70_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9615461.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEK"
BASE COUNT         2303 a         1154 c         1444 g         1878 t
ORIGIN      
        1 acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat gattattgca
       61 ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa aaatttcggg
      121 acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt aggtgcgacg
      181 ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg agcagcgtta
      241 ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat ccctaaaaag
      301 gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt tactgccttc
      361 cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc ggggatgagt
      421 ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg gttcaaacaa
      481 aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta tctgctgatc
      541 gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt atctggaatc
      601 attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat ctctgtgttt
      661 gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt tacactgaat
      721 gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc agtatgggga
      781 gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc tgtcatgctt
      841 tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg cagtaaaaag
      901 ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa gggaacagtc
      961 agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt ttaccagcgg
     1021 tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat agggatcatt
     1081 gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa tgctttgatg
     1141 attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga tcgtaataca
     1201 aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat acgtgatctg
     1261 tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca agcactgatt
     1321 ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct ttcttctaat
     1381 ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac aagccagatt
     1441 ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat cgttctccat
     1501 ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag tgatgaagat
     1561 atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga aagtttagat
     1621 aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca acgcaaactt
     1681 gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca gcaagatagt
     1741 ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat tgatgaatac
     1801 gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa tatcaatttg
     1861 ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt tgcgtttcga
     1921 gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa gttttataaa
     1981 aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag aacacaaaaa
     2041 gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa tatccaagat
     2101 aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact tatggtgaaa
     2161 aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc gggttgattg
     2221 ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc gatagcagcg
     2281 acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat ctttctcaag
     2341 cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga aacagcccct
     2401 tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa aacggagaag
     2461 accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa gaagatgctt
     2521 ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt gaattagata
     2581 aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg caagtgttga
     2641 ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat tatgaagcga
     2701 tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg gttacccacg
     2761 atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt ggaaaattat
     2821 acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt gatcgtgtag
     2881 aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct tggatgaggg
     2941 ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc gaagatctaa
     3001 aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta gcgacacaac
     3061 gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat gaccaaacac
     3121 tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt actggtaaaa
     3181 atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc ctggatagcg
     3241 gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa aatgaagaaa
     3301 tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa gcaaaaacag
     3361 cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt ccaagattta
     3421 tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta tttttgttga
     3481 aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat gatttggaca
     3541 tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct gtgatcactg
     3601 tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta ttccaaggaa
     3661 acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag caaaaagaga
     3721 cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct gctgaaaaga
     3781 agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag attgcagaac
     3841 ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat aatttcactc
     3901 gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa gaaaaaatgg
     3961 cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga tggaacaagc
     4021 gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag acagaacagg
     4081 cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa aagggtttcc
     4141 tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc tttggttttt
     4201 aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt gggatgaatg
     4261 ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga ctgattttgg
     4321 ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga gcaagctttt
     4381 ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc ttggacacat
     4441 ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg atcaaatcaa
     4501 acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg tttctgcatg
     4561 gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt ttcaatttta
     4621 tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg tgtttctagg
     4681 cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac atgaaacagg
     4741 tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata tcaatcatat
     4801 cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt tagttttgaa
     4861 tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag aaggatatga
     4921 cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat gagaaaatgt
     4981 tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac cgtcttcctt
     5041 ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat acactagtga
     5101 tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt cagacgattg
     5161 ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat agtgtggaag
     5221 acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga gggtcagctg
     5281 tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc catcatgtat
     5341 tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg atcaatacta
     5401 gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg tatcagcgta
     5461 atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc tatggataga
     5521 ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa gtaaagaata
     5581 aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa acaacatgca
     5641 tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa agtccgcctg
     5701 ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca gcagccacga
     5761 cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact ctctcccgtt
     5821 tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag attaatacaa
     5881 ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta taacttcctg
     5941 caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt gcttttgtaa
     6001 aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc gatccataga
     6061 tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact aaaagaacca
     6121 ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta aacacttcat
     6181 ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct cctttactag
     6241 gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat tgtcttacat
     6301 gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat aatgacaaat
     6361 gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga tgctttaggc
     6421 attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga tgatgaccaa
     6481 ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc caaaacaagc
     6541 caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga cggcagtgaa
     6601 gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc aagacaagcc
     6661 agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga ccaaggatta
     6721 tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt agagaaaat
//
LOCUS       DACDTO010000258         5984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.121, whole genome shotgun sequence.
ACCESSION   DACDTO010000258 DACDTO010000000
VERSION     DACDTO010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.121"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            complement(41..1027)
                     /locus_tag="IWK70_003011"
                     /pseudo
     CDS             complement(41..1027)
                     /locus_tag="IWK70_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006701269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1179..1874
                     /gene="vanR"
                     /locus_tag="IWK70_003012"
     CDS             1179..1874
                     /gene="vanR"
                     /locus_tag="IWK70_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9615462.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1852..3006
                     /locus_tag="IWK70_003013"
     CDS             1852..3006
                     /locus_tag="IWK70_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9615463.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3221..4189
                     /locus_tag="IWK70_003014"
     CDS             3221..4189
                     /locus_tag="IWK70_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAP9615464.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4182..5213
                     /gene="vanA"
                     /locus_tag="IWK70_003015"
     CDS             4182..5213
                     /gene="vanA"
                     /locus_tag="IWK70_003015"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAP9615465.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5219..5827
                     /locus_tag="IWK70_003016"
     CDS             5219..5827
                     /locus_tag="IWK70_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAP9615466.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT         1811 a         1159 c         1348 g         1666 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccct atttccgata accgtgtatt
       61 gaaaggcatc ttcgaagcca ttatccaata tttcgcaggc ttttgtatat ttaggctggt
      121 cgatatattc accgactaag gcattcttag ctgttcgagc gagttcaata tccgtaaact
      181 taaagatcgc ttttactgct tctctaaacg gttttgaatt cttttttgga atggaactga
      241 agatgtttct taaaaaatgg acctggcatc tctgccaact tgcgttggta aatgacttac
      301 gaatcgcaga cactaggcct ttatgggcat cagaaatgat gagttctgtc ccctttaggc
      361 cgcgttcttt taagtattca aagaagatgg accatgtgtc atcactttct tcattttgaa
      421 tcatgaagcc aatgatttca cggtcgccac cttctgttat cccgatcgca atatggcagc
      481 ttttagaaag cactcgatgg tcctcacgga cttttatgta gagaacatca gtcatcagat
      541 aaggataatt cgtacctgag agtgaacggt tctgccattc gttgaccatc gggtctaact
      601 gctcagtcag gctagaaaca aaagatttcg atacagattt tccacatagc tcttcaacaa
      661 tctttgaaac tttacgtgtc gaaacgcccg aaacatacat ctcaagcatt gaagcgagca
      721 gtgccttttc atttcgctga taacgctcaa acaccgtcgg tgaaaattca ccatcacgtg
      781 ttctaggcac ttttaattcg agtgtaccca cacgagtcgt aaagtctcgc tcataatagc
      841 catttctttg actctgacga ctttctgaac gttcatagtc atcggcttga atatattctg
      901 ttcgttgatt ttccatcaat tggttgaaaa cagtggttaa aatattttta gaaacatcat
      961 cttttaccga atgttcaata atactttgaa cctcttcgtt gttcagtgta aaatgtactt
     1021 gggtcatgta acgtcctcct gggtatgttt ttgtggttaa aaacattgta ccgtaaaagg
     1081 gctgttacat ggccttttat cttttacaca attatatgga ctttatccta taatctgata
     1141 aattattaag ggcaaactta tgtgaaaggg tgataactat gagcgataaa atacttattg
     1201 tggatgatga acatgaaatt gccgatttgg ttgaattata cttaaaaaac gagaattata
     1261 cggttttcaa atactatacc gccaaagaag cattggaatg tatagacaag tctgagattg
     1321 accttgccat attggacatc atgcttcccg gcacaagcgg ccttactatc tgtcaaaaaa
     1381 taagggacaa gcacacctat ccgattatca tgctgaccgg gaaagataca gaggtagata
     1441 aaattacagg gttaacaatc ggcgcggatg attatataac gaagcccttt cgcccactgg
     1501 agttaattgc tcgggtaaag gcccagttgc gccgatacaa aaaattcagt ggagtaaagg
     1561 agcagaacga aaatgttatc gtccactccg gccttgtcat taatgttaac acccatgagt
     1621 gttatctgaa cgagaagcag ttatccctta ctcccaccga gttttcaata ctgcgaatcc
     1681 tctgtgaaaa caaggggaat gtggttagct ccgagctgct atttcatgag atatggggcg
     1741 acgaatattt cagcaagagc aacaacacca tcaccgtgca tatccggcat ttgcgcgaaa
     1801 aaatgaacga caccattgat aatccgaaat atataaaaac ggtatggggg gttggttata
     1861 aaattgaaaa ataaaaaaaa cgactattcc aaactagaac gaaaacttta catgtatatc
     1921 gttgcaattg ttgtggtagc aattgtattc gtgttgtata ttcgttcaat gatccgaggg
     1981 aaacttgggg attggatctt aagtattttg gaaaacaaat atgacttaaa tcacctggac
     2041 gcgatgaaat tatatcaata ttccatacgg aacaatatag atatctttat ttatgtggcg
     2101 attgtcatta gtattcttat tctatgtcgc gtcatgcttt caaaattcgc aaaatacttt
     2161 gacgagataa ataccggcat tgatgtactt attcagaacg aagataaaca aattgagctt
     2221 tctgcggaaa tggatgttat ggaacaaaag ctcaacacat taaaacggac tctggaaaag
     2281 cgagagcagg atgcaaagct ggccgaacaa agaaaaaatg acgttgttat gtacttggcg
     2341 cacgatatta aaacgcccct tacatccatt atcggttatt tgagcctgct tgacgaggct
     2401 ccagacatgc cggtagatca aaaggcaaag tatgtgcata tcacgttgga caaagcgtat
     2461 cgactcgaac agctaatcga cgagtttttt gagattacac ggtataacct acaaacgata
     2521 acgctaacaa aaacgcacat agacctatac tatatgctgg tgcagatgac cgatgaattt
     2581 tatcctcagc tttccgcaca tggaaaacag gcggttattc acgcccccga ggatctgacc
     2641 gtgtccggcg accctgataa actcgcgaga gtctttaaca acattttgaa aaacgccgct
     2701 gcatacagtg aggataacag catcattgac attaccgcgg gcctctccgg ggatgtggtg
     2761 tcaatcgaat tcaagaacac tggaagcatc ccaaaagata agctagctgc catatttgaa
     2821 aagttctata ggctggacaa tgctcgttct tccgatacgg gtggcgcggg acttggattg
     2881 gcgattgcaa aagaaattat tgttcagcat ggagggcaga tttacgcgga aagcaatgat
     2941 aactatacga cgtttagggt agagcttcca gcgatgccag acttggttga taaaaggagg
     3001 tcctaagaga tgtatataat tttttaggaa aatctcaagg ttatctttac tttttcttag
     3061 gaaattaaca atttaatatt aagaaacggc tcgttcttac acggtagact taataccgta
     3121 agaacgagcc gttttcgttc ttcagagaaa gatttgacaa gattaccatt ggcatccccg
     3181 ttttatttgg tgcctttcac agaaagggtt ggtcttaatt atgaataaca tcggcattac
     3241 tgtttatgga tgtgagcagg atgaggcaga tgcattccat gctctttcgc ctcgctttgg
     3301 cgttatggca acgataatta acgccaacgt gtcggaatcc aacgccaaat ccgcgccttt
     3361 caatcaatgt atcagtgtgg gacataaatc agagatttcc gcctctattc ttcttgcgct
     3421 gaagagagcc ggtgtgaaat atatttctac ccgaagcatc ggctgcaatc atatagatac
     3481 aactgctgct aagagaatgg gcatcactgt cgacaatgtg gcgtactcgc cggatagcgt
     3541 tgccgattat actatgatgc taattcttat ggcagtacgc aacgtaaaat cgattgtgcg
     3601 ctctgtggaa aaacatgatt tcaggttgga cagcgaccgt ggcaaggtac tcagcgacat
     3661 gacagttggt gtggtgggaa cgggccagat aggcaaagcg gttattgagc ggctgcgagg
     3721 atttggatgt aaagtgttgg cttatagtcg cagccgaagt atagaggtaa actatgtacc
     3781 gtttgatgag ttgctgcaaa atagcgatat cgttacgctt catgtgccgc tcaatacgga
     3841 tacgcactat attatcagcc acgaacaaat acagagaatg aagcaaggag catttcttat
     3901 caatactggg cgcggtccac ttgtagatac ctatgagttg gttaaagcat tagaaaacgg
     3961 gaaactgggc ggtgccgcat tggatgtatt ggaaggagag gaagagtttt tctactctga
     4021 ttgcacccaa aaaccaattg ataatcaatt tttacttaaa cttcaaagaa tgcctaacgt
     4081 gataatcaca ccgcatacgg cctattatac cgagcaagcg ttgcgtgata ccgttgaaaa
     4141 aaccattaaa aactgtttgg attttgaaag gagacaggag catgaataga ataaaagttg
     4201 caatactgtt tgggggttgc tcagaggagc atgacgtatc ggtaaaatct gcaatagaga
     4261 tagccgctaa cattaataaa gaaaaatacg agccgttata cattggaatt acgaaatctg
     4321 gtgtatggaa aatgtgcgaa aaaccttgcg cggaatggga aaacgacaat tgctattcag
     4381 ctgtactctc gccggataaa aaaatgcacg gattacttgt taaaaagaac catgaatatg
     4441 aaatcaacca tgttgatgta gcattttcag ctttgcatgg caagtcaggt gaagatggat
     4501 ccatacaagg tctgtttgaa ttgtccggta tcccttttgt aggctgcgat attcaaagct
     4561 cagcaatttg tatggacaaa tcgttgacat acatcgttgc gaaaaatgct gggatagcta
     4621 ctcccgcctt ttgggttatt aataaagatg ataggccggt ggcagctacg tttacctatc
     4681 ctgtttttgt taagccggcg cgttcaggct catccttcgg tgtgaaaaaa gtcaatagcg
     4741 cggacgaatt ggactacgca attgaatcgg caagacaata tgacagcaaa atcttaattg
     4801 agcaggctgt ttcgggctgt gaggtcggtt gtgcggtatt gggaaacagt gccgcgttag
     4861 ttgttggcga ggtggaccaa atcaggctgc agtacggaat ctttcgtatt catcaggaag
     4921 tcgagccgga aaaaggctct gaaaacgcag ttataaccgt tcccgcagac ctttcagcag
     4981 aggagcgagg acggatacag gaaacggcaa aaaaaatata taaagcgctc ggctgtagag
     5041 gtctagcccg tgtggatatg tttttacaag ataacggccg cattgtactg aacgaagtca
     5101 atactctgcc cggtttcacg tcatacagtc gttatccccg tatgatggcc gctgcaggta
     5161 ttgcacttcc cgaactgatt gaccgcttga tcgtattagc gttaaagggg tgataagcat
     5221 ggaaatagga tttacttttt tagatgaaat agtacacggt gttcgttggg acgctaaata
     5281 tgccacttgg gataatttca ccggaaaacc ggttgacggt tatgaagtaa atcgcattgt
     5341 agggacatac gagttggctg aatcgctttt gaaggcaaaa gaactggctg ctacccaagg
     5401 gtacggattg cttctatggg acggttaccg tcctaagcgt gctgtaaact gttttatgca
     5461 atgggctgca cagccggaaa ataacctgac aaaggaaagt tattatccca atattgaccg
     5521 aactgagatg atttcaaaag gatacgtggc ttcaaaatca agccatagcc gcggcagtgc
     5581 cattgatctt acgctttatc gattagacac gggtgagctt gtaccaatgg ggagccgatt
     5641 tgattttatg gatgaacgct ctcatcatgc ggcaaatgga atatcatgca atgaagcgca
     5701 aaatcgcaga cgtttgcgct ccatcatgga aaacagtggg tttgaagcat atagcctcga
     5761 atggtggcac tatgtattaa gagacgaacc ataccccaat agctattttg atttccccgt
     5821 taaataaact tttaaccgtt gcacggacaa actatataag ctaactcttt cggcaggaaa
     5881 cccgacgtat gtaactggtt cttagggaat ttatatatag tagatagtat tgaagatgta
     5941 aggcagaggg ttctgttgca aagttttaaa taaagaataa aatc
//
LOCUS       DACDTO010000259         5895 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.123, whole genome shotgun sequence.
ACCESSION   DACDTO010000259 DACDTO010000000
VERSION     DACDTO010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.123"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..2103
                     /locus_tag="IWK70_003017"
     CDS             <1..2103
                     /locus_tag="IWK70_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9615467.1"
                     /translation="KLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTY
                     FLIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQA
                     AKEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQ
                     LSFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQ
                     DYELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFE
                     VQPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREII
                     EAQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMR
                     AKPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAA
                     TGVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQM
                     ARAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRG
                     DMLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQ
                     PQDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRK
                     VFIEQEETSETLPDDNDM"
     gene            2333..3400
                     /gene="rlmN"
                     /locus_tag="IWK70_003018"
     CDS             2333..3400
                     /gene="rlmN"
                     /locus_tag="IWK70_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9615468.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3593..4696)
                     /locus_tag="IWK70_003019"
     CDS             complement(3593..4696)
                     /locus_tag="IWK70_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9615469.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5249..5797
                     /gene="aac(6')"
                     /locus_tag="IWK70_003020"
     CDS             5249..5797
                     /gene="aac(6')"
                     /locus_tag="IWK70_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9615470.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1966 a         1024 c         1273 g         1632 t
ORIGIN      
        1 aaactccata ctggggaacc aaatatcatg gggacgacat gggatttgtt agccagtgat
       61 ataaaacaaa gtcaagtaga caataacgtt ggtggtggga tgattggagc aatcctttat
      121 cattttactt acttcttgat tgcacagcct ggaagttatt tagcagcagt gctattactg
      181 gcaggaggag cattcctgtt cagtaatctg gaaggctatc agctgttaaa tggtatccaa
      241 tcaattggag aacgagtaca ggaattattg gaaggtgatc ctgccaagca agctcgaaag
      301 caagcggcaa aggaagagag aatgaaacag cgcgcagaag cgaaagaagc aaggcgctta
      361 gctgcaaaag aagctgcaga aaaagaagct gcagaatatg agaagaaaaa agcggtgagc
      421 cagcaaaggg aaaatactgc tgatgaatgg caggaaccag aaaatacaga gccggagcag
      481 ctaagttttg ttccaatcga cagttttcaa gaaaatatcc atcctgcgaa tctagagaaa
      541 ccagtaccag atacaccaaa acagaccaat acagcagaag gtttttcaga tgagttgcca
      601 gaggatgacg gtacctcatt agaatttgag attgaagcag aacaggaaaa tcaagactat
      661 gaattaccga cagttgattt gcttgattct ataccaactg tggatcaaag tgatgagtat
      721 aaaaaaattg aaaaaaatat cggcgtatta gaacaaactt tccaaagttt cggtgtcgat
      781 gccaaggtcg tcaaagccag cttaggacca tccgtcacaa aattcgaagt ccagccggct
      841 gtaggggtaa aagtaagtaa aattgtgaac ttaacagatg atatcgcgct tgctttagcg
      901 gcgaaagatg tccggatgga agcaccgatt ccaggtaaat cactgattgg tattgaagta
      961 ccaaatggaa agatcagtat ggtttccttt agagaaatca tcgaagcaca accaaaccac
     1021 ccagataaat tactagaagt acctttagga cgtgatgttt ctggacgggt acaaacggct
     1081 gatctttcga agatgcctca cttactcgtg gctggttcga ctggtagtgg gaagtcagta
     1141 gcaattaatg ggattatcac aagtatatta atgcgtgcta agccccatga agtaaaactg
     1201 atgatgatcg atccgaaaat ggttgaattg aatatgtaca acggcatacc tcatctgttg
     1261 acgccggtcg tgacgaaccc tagaaaagca gcgcaagcct tacaaaaagt agtacaagaa
     1321 atggaagaac gatatgaaaa attcgcggca accggagtaa gaaatatttc tggttacaat
     1381 gaatttgttc aacagaaaaa cttggaaaac ggaacgaaac atccaacctt accattcatc
     1441 gtagtgatcg tagatgaatt agctgatttg atgatggtag ccagcaatga agttgaagat
     1501 gcgattattc gtttagcgca aatggctcgg gcagcgggga ttcatatgat cctagctact
     1561 caacgtccaa gtgtggatgt tatcacagga atcatcaaag caaatgtacc ttccagaatg
     1621 gcatttgcgg tatcaagcgg tacagattct cggacgatca ttgattctaa tggggcagaa
     1681 aagctcttag ggcgaggaga tatgctattc ttgccaatgg gtgagaataa accgatccgt
     1741 gtgcaaggag cattcatttc tgaccacgaa gtagaacggg tcgttcaatt tgttacagat
     1801 cagcaagaag ctcattatga agagaaaatg atgccgacag atgaagttga aacgacaggt
     1861 gctccagaac agccacaaga tgagctgttt gaagaagcaa aagcacttgt cgttgaaatg
     1921 caaacggcaa gtatttccct tctccaaaga agatttcgga tcggttacaa ccgagcagca
     1981 cgtttagtag atgaactgga agctcatgga gttgttggcc cgtcagaagg aagcaagcct
     2041 cggaaagtat tcattgagca ggaagaaaca tcagaaacgt tgccggacga taatgacatg
     2101 taaaacttgt gtgatgaagg gttgaaaaac gaataaccaa taaaaagaca aggcatgatg
     2161 ataccaaaag aaaaaatggt ttttttccat cttttctaat acggtatgat gtcttgtctt
     2221 tttagttttt gtgagattaa agttctattc gatttatttg tgaagtattg cattaaggaa
     2281 atgaatcatg tatagtatgt agcgaaactt tgagaaaaag agggaaaata ctttggaaaa
     2341 acagtccatt tatggcttga cgaatgaaga attaatcaat tggtttatag aaaacggcga
     2401 gaaaaaattc cgagctgccc aagtttggga atggttgtat caaaaacgtg taagtaattt
     2461 cacagaaatg acgaatctat cgaaacaatt gatcgaaaaa ttatcggagc attttatcat
     2521 caatcctcta aaacagatgg tcgtccaaga agcttcggat ggtacagtaa aatatttgtt
     2581 tgaactccct gataaaaaca tgattgagac tgtactgatg agacaagaat atggcttgtc
     2641 agtttgtgtg acgacacaag taggatgtaa tatcggctgt acgttttgtg caagcgggct
     2701 gctcaaaaag aaccgtgatc tgactgctgg agaaattgtt gcacagatca tgatggtcca
     2761 acattatttt gatgagcgca atcttggcga acgcgtttca catgtcgttg taatggggat
     2821 cggtgagcca tttgataatt acgacaatgt aatggacttt ttgcatatca tcaatgatgc
     2881 caaaggattg gcaattggtg ctcggcacat tacagtttcc actagtggat tagcacacaa
     2941 aatcaaggaa ttcgctaata acggcttgca agtcaatctg gctatttcct tgcacgcgcc
     3001 gaataatgag gtgcgaacat cgatcatgcg gatcaaccga agtttcccaa ttgagaaatt
     3061 gatggaagct gtggatgagt atttagagaa aacaaaccgt cgaatcacgt ttgaatatat
     3121 catgttgaat caagtaaatg atagaccaga gcatgcgcaa caattagcag atctattaaa
     3181 agataagaaa aaattagcat atgtcaactt gattccatat aatccagtca gtgaacatga
     3241 tcaatattct cgcagtccaa aagctgatgt attgagattt tatgatgtat tgaagaaaaa
     3301 cggcgtaaac tgtgtgatac gtaaagaaca tggtacagat attgatgcag cttgcggaca
     3361 attgcgaagc aagcaaatga aaaaaacagt tgccaaataa tcgtatccta ctcgtttttt
     3421 caattaccat aaaaggagca gttttcttaa ttaaggaact gttcaactta aataagagaa
     3481 aagattctat agcgaaagct ataaagcttt ttttgacaag aaaaaccctc atcaagcaaa
     3541 gaaatgtcct caatttgatg acagcctgct tgatgggggt tttagcaatt atttaactga
     3601 ttttcttata tccttgctgg ctttcttgtg tagcatgata agtttctacg tcaagaatgg
     3661 cctgtttaaa tttttctcgg gtgatttcat aaggcacatg tttaatatca tttgttgcca
     3721 tggctttatc taagattgct tctaaatcgg tcgtgacatt gagattcatg tcggttaacg
     3781 atactggaag ttggttcttt ttcataaaca catagatttt atcaaacaaa tcgaactgct
     3841 gatccatcat caataaggtc agtacaccat aactgacaga tgctccgtga aggtatccct
     3901 ccattttttc tagaacagtc gtaccgtaat agaatgagtg tgccatagca gaattatagt
     3961 tattgttaac taaaacagaa accaatccgg tagtaacaat gatatccgaa acgacttgtt
     4021 caattgcata tgagggacga tcgtcggcac tatctttcaa tgcttgcgta ccataagtca
     4081 agagacgatc aatgcacatt tttccgattt ggattcctaa tctatccgtg tagcctagat
     4141 catcgccacg agcagaaaaa gtagattcga cttctttgga taaagcatcg ccaatacctg
     4201 cccataagta tttatcaggt gcttcggcaa taattgctgt atcaatgaag gtgtgtacag
     4261 gtggttttga aggaaagaag agtccgtcca ttacgccttc ttcgttatag atgacacata
     4321 ccgcagtgac gggcgaacag ttcgaaccaa tagtgggaaa ggtgaagact ggtttagcta
     4381 gtttttcacc aatgactttt acagtatcgc aagcacggcc accaccgact gcaaacagga
     4441 cgtctgcttt ttggacactt tcatgctgga tcaactgttc aatatttcca taagttgaat
     4501 tacccccgta ccagagactg gcgacaacct ctatttcctg tgtcaaagct acttttattt
     4561 tgttttctgc ctttgaaagt gcagtcttcc ctccaatcag cactactttt gtcccatatt
     4621 ctttgacgat tccatttgct ttttcatacg catctgttcc aatggtataa tttggtaaga
     4681 aaacactttg actcatattc atttccttct ttcttcgttg aaacgggtta aaattttaaa
     4741 gagtgatcga tctgactgcg gctgtagatg gagtagaggt gaacctaaga caggataagt
     4801 gatgaataag gtggcatgaa caccttgaaa tcatcaataa gtatcctaaa aacgaacaag
     4861 tacaaaaaca ttgaaaacga caataatgaa tttttgttcc tcttagtatt tctaccgatt
     4921 ccctttgctt ttttgtaatt atctaaaaat tctaaaaatt aagattatta tatgaaaaca
     4981 ggttttattt gtcaattcat aattggtttt ttttaaagca aaagataatc tatatcatga
     5041 tgaacaaaca gagttttttc ctccaattga tggctttcct gatctttcct attttgaata
     5101 aagtgtttct gtataatttt tttattttat aagtaagata aataatgaaa gaatagagat
     5161 actgattggt agctaaagat ttttgagcga gaacatttcg ttttttcaaa aggactgtta
     5221 gaataagcaa gaacaagggg gaagacgtat gataatcagt gaatttgacc gtaataatcc
     5281 agtattgaaa gatcagcttt ctgatttact gagactgact tggccggaag aatatggaga
     5341 cagctcggca gaagaagtag aagaaatgat gaatccagaa cgaatcgcgg tagcagcggt
     5401 agaccaagat gagttagtag gatttattgg tgcaatccct caatacggta tcacaggttg
     5461 ggaattgcat ccattagttg tagaaagctc ccgacgaaag aaccaaatag gtactcgatt
     5521 agtcaattac ttagaaaaag aagtagcttc cagaggagga atcacgattt atttaggtac
     5581 ggatgattta gaccatggaa caacgttaag tcaaacggac ctgtatgagc atacatttga
     5641 taaagtggct tccatccaga accttcgtga acatccgtat gaattctatg aaaaattagg
     5701 ttataaaatc gtaggtgtct taccaaatgc aaatggctgg gacaaaccgg atatttggat
     5761 ggcaaaaacg attattcctc gaccaaattc tcaataataa aaagaggttg tgacagaagt
     5821 aatcagcttc aagaaataag aaggaattta cgaaaattac ttcttgaatt tttgtgaaat
     5881 tccaacttat ttccg
//
LOCUS       DACDTO010000260         3179 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.153, whole genome shotgun sequence.
ACCESSION   DACDTO010000260 DACDTO010000000
VERSION     DACDTO010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.153"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            <1..873
                     /locus_tag="IWK70_003021"
     CDS             <1..873
                     /locus_tag="IWK70_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9615471.1"
                     /translation="SHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFF
                     LKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGE
                     LNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRY
                     AHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCR
                     LPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSR
                     VKERLEQIIGSLTG"
     gene            880..1257
                     /locus_tag="IWK70_003022"
     CDS             880..1257
                     /locus_tag="IWK70_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9615472.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            1411..2193
                     /locus_tag="IWK70_003023"
     CDS             1411..2193
                     /locus_tag="IWK70_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9615473.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(2319..3050)
                     /gene="erm(A)"
                     /locus_tag="IWK70_003024"
     CDS             complement(2319..3050)
                     /gene="erm(A)"
                     /locus_tag="IWK70_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9615474.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(3108..>3179)
                     /locus_tag="IWK70_003025"
     CDS             complement(3108..>3179)
                     /locus_tag="IWK70_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
                     /protein_id="HAP9615475.1"
                     /translation="GDKSMGTFSIFVINKVRYQPNQN"
BASE COUNT         1124 a          467 c          602 g          986 t
ORIGIN      
        1 agtcatcttg ataaattacc cgaatatata gctacgatga ctatgattgt tcaagaatgt
       61 ggaatgagga taagtgaatt gtgcaccttg aaaaaaggct gtctattaga ggacaaagat
      121 ggagatttct ttttaaagta ttatcaatgg aaaatgaaaa aggagcatat agttccaata
      181 tctaaagagg tagctttact tattaaagtt cgggaagata aagtttcaga ggaatttcca
      241 gatagtgaat acctctttcc aagaaaagat ggatcgccat taaaacaaga aacatttaga
      301 ggtgagttaa ataaattagc ttatgagcaa aatatagtgg ataaatcagg tgagatttat
      361 agattccatg cccatgcctt tcgccataca gtaggaacaa gaatgattaa caacgggatg
      421 ccccagcata ttgtgcagaa atttttgggg catgaaagcc cagaaatgac aagcagatac
      481 gctcatatct ttgatgaaac tctaaaaaat gaatttacta aatttcagga aaaactggtt
      541 accaataatg gagatgtgct tgatctagat gaagataatg aagtcgatga tgtagagctt
      601 caatggttca agaaaaatat aaatgcacaa gtgcttccaa atggttattg tagattgcca
      661 gtagtagcag gtggttgtcc acatgcgaat gcatgcttag attgcactca cttctgtacc
      721 agtaagcaat tcttaccaca gcacgaagaa cagttagagc gtacagaaga gttattagcc
      781 atagctaagg ataaacaatg gcaaagacaa gtagagacta atagccgtgt taaagagcgt
      841 ttagaacaaa tcattggaag tttgacgggg taattatcaa tggataaaca agttagaaat
      901 acaacagaaa ttgtacgttt ggcgaagcag aaatcaaaaa agacaaggga aaaagtagac
      961 aaagcgattt ctaaattttc gattgaaggt aaagttatta attttaattc aatagcaaag
     1021 gaagctaatg tttctaaatc atggctttat aaggaacacg atattaggca aagaatcgaa
     1081 tcccttcgtg agcgtcaaat aacagcaaat gtagtctcaa aacccaagaa aagttctcgt
     1141 tcggaggaaa tccttattaa aaccttaaaa agaagagtaa tggaattaga aaaagaaaat
     1201 aaaaaattac agaaccaaat tcaaaaatta tatggagatc tgtataataa agaataatta
     1261 ttaatctgta gacaaattgt gaaaggatgt acttaaacgc taacggtcag ctttattgaa
     1321 cagtcattta ggtatatgtc caatctaggg taagtaaatt gagtatcaat ataaacttta
     1381 tatgaacata atcagttacg aggtgaaatc atgagcaatt tgattaacgg aaaaatacca
     1441 aatcaagcga ttcaaacatt aaaaatcgta aaagatttat ttggaagttc aatagttgga
     1501 gtatatctat ttggttcagc agtaaatggt ggtttacgca ttaacagcga tgtagatgtt
     1561 ctagtcgtcg tgaatcatag tttacctcaa ttaactcgaa aaaaactaac agaaagacta
     1621 atgactatat caggaaagat tggaaatacg gattctgtta gaccacttga agttacggtt
     1681 ataaatagga gtgaagttgt cccttggcaa tatcctccaa aaagagaatt tatatacggt
     1741 gagtggctca ggggtgaatt tgagaatgga caaattcagg aaccaagcta tgatcctgat
     1801 ttggctattg ttttagcaca agcaagaaag aatagtattt ctctatttgg tcctgattct
     1861 tcaagtatac ttgtctccgt acctttgaca gatattcgaa gagcaattaa ggattctttg
     1921 ccagaactaa ttgaggggat aaaaggtgat gagcgtaatg taattttaac cctagctcga
     1981 atgtggcaaa cagtgactac tggtgaaatt acctcgaaag atgtcgctgc agaatgggct
     2041 atacctcttt tacctaaaga gcatgtaact ttactggata tagctagaaa aggctatcgg
     2101 ggagagtgtg atgataagtg ggaaggacta tattcaaagg tgaaagcact cgttaagtat
     2161 atgaaaaatt ctatagaaac ttctctcaat taggctaatt ttattgcaat aacaggtgct
     2221 tacttttaaa actactgatt tattgataaa tattgaacaa tttttgggaa gaataaagcg
     2281 tcctcttgtg aaattagaga acgctttatt actttaattt agtgaaacaa tttgtaacta
     2341 ttgaaaatag aaagaaattg ttccttcgat agtttattaa tattagtgac atttgcatgc
     2401 ttcaaagcct gtcggaattg gtttttagtg aaaagaacac gatattcacg gtttacccac
     2461 ttataaacaa aagatcgata ctttttgtag tccttctttg aaatcaatgg ttgatgtcgt
     2521 tcaagaacaa tcaatacaga gtctacactt ggcttaggat gaaaatatag tggtggtact
     2581 tttttgagca tttttatatc catctccacc attaatagta aacccaaagc tcgttgcaga
     2641 ttttgcaatc ttttcgcaaa tcccttctca acgataagat agctatattt agcctgactt
     2701 tcaaaggtaa ttcttttgac aatatccgta ctgatgttat aaggaatatt accatatatc
     2761 ttatagttta tatgttttgg gaaggaaaat tttagaatat ccgtttgaat cacttttata
     2821 ttctcagagg ggtttaccgc ttctttagtc acttgacata agcctccatc aatttctata
     2881 gcagtaactg atcgactcat tttgactagc tctttggtaa aatgtccttt tcctgatccg
     2941 atttctatta cgttgtcttg tttactgata ttcgtgtgat tcaatatttc ttttacatgc
     3001 tttttagaag taataaaatt ttgcgtgtct ttagggtttt tctggttcat tataaccttc
     3061 tccttactgg ttataatgaa ctgacattaa gcgttcatta taaccaatta attttgattt
     3121 ggttgataac gaactttatt aataacaaat atagaaaaag tacccatact tttatctcc
//
LOCUS       DACDTO010000261         3027 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.156, whole genome shotgun sequence.
ACCESSION   DACDTO010000261 DACDTO010000000
VERSION     DACDTO010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.156"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..1524
                     /gene="msr"
                     /locus_tag="IWK70_003026"
     CDS             46..1524
                     /gene="msr"
                     /locus_tag="IWK70_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9615476.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1571..2017
                     /locus_tag="IWK70_003027"
     CDS             1571..2017
                     /locus_tag="IWK70_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9615477.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2216..2731)
                     /locus_tag="IWK70_003028"
     CDS             complement(2216..2731)
                     /locus_tag="IWK70_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9615478.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            2848..>3027
                     /locus_tag="IWK70_003029"
     CDS             2848..>3027
                     /locus_tag="IWK70_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9615479.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETT"
BASE COUNT          972 a          523 c          632 g          900 t
ORIGIN      
        1 agtatcgata attgttgttt tacatgaaat aggagtgtaa aacatatgga aaatttagca
       61 gtaaatataa caaatctaca agtcagcttt ggcaaccagc tagaactatc tattgattct
      121 cttcgtgtct atcagcaaga tcgtataggg atcataggag aaaatggtgt cggtaaatcg
      181 actttgctca aactaatagc cggtgaactt tttcccgatc atgggaaaat ccaaacagag
      241 atcaccttca actacctgcc tcaattaacc tatctcgctg aagcaaagga cctaaatttg
      301 gaattagcca gtcatttcca gttaagactg gaagaaactt cagagcggaa atggagcgga
      361 ggggaagaac gaaagatcga gttgatacgt cttctttctt cttatgaaca agggatgctt
      421 ctagacgagc caacaaccca tctagataga aaaagtattg atcgactgat tgaagagctt
      481 cgttattatt atggtacgct ggtttttgtt agtcatgatc gctattttct agatgagcta
      541 gcatcgaaaa tctgggaagt aaaagacgga gaaatccgag agttttcggg gaattatagt
      601 gcctatctca ctcaaaagga attggagaaa aagactcagc tacgagaagc agagtcgatc
      661 atgaaagaga aaaaacgatt ggaaaaatcg atccaagaaa agaaaaaaca agcggaaaag
      721 ttggaaaaag tgtccagtaa aaagaaaaag caacaaatca gactggatcg gttgtcttcc
      781 tctaaacaaa aagacagtgt acaaaaagcc atccaaaaga atgcgaaaac attagagaga
      841 agactccaaa aaataggaga aacaaccaag ccgcagcaga tgaaacaaat ccgttttcca
      901 gtaccaaaat ctcttgaact ccacagccgc tatcccatca tgggacaaaa tgtccaattg
      961 gaaagaagtg ggagaacatt actggtaaat ggtgattttc agttttcttt aggtaaaaaa
     1021 atcgcgattg tcggtgaaaa tggttcaggt aagacaacct tattagaaca tatccgcaaa
     1081 caaggagaag gaatccttct ctctccgaaa gtaagctttc aagtatatca gcaaaagggt
     1141 tatcaaatga catctgaaga atccatcatt cgttttgtca tgagacaaac agagttttca
     1201 gaatcgcttg tccgtagttt gctgaatcac ttagggtttg ctcaggaaac tttgacgaaa
     1261 ccgttatgta cattaagtgg gggagaagcg acccgtctga cgattgcttt gctttttact
     1321 aagccaagta atgtgttgct gttagatgaa ccgactaatt ttattgatat ggcaacgatc
     1381 gaagctttag agaagctaat gcaaatatat ccgggaacga ttttgtttac ttctcatgat
     1441 tcttactttg tcgagcgtac ggctgatgaa gtttatgaaa taaaagggca gaaaataaaa
     1501 aaagtactta cgagaaattt ttaaatgtgc tacgatgatt ttcgtgacaa atttaaagga
     1561 gtggtacaga ttgcctacaa ataaaaaaga aggaataatt tttacaacaa tgatgtgttt
     1621 tttgatggtc ttggggatga gcgcgtataa tttatggctg catcaagatc ttcatttatc
     1681 aagtttgttg attggtctag tgcctggatt cgtggtggct ttcattttgg atgtcttcat
     1741 catcggtgtg ctggcgaaaa aaattgcctt taaactacca gtaaatccag aaagcaaggt
     1801 ccaaatgatc ttaactattt catgcttgat ggttttagga atggtgacat gtatgtcttt
     1861 attcggtgtg atcatggaac aaggaatacc tgatcaatta ttctctgctt atatgaatgc
     1921 ttggaaaatg aatgtgattg ccgctttacc gttgcagttg atcatcgttg gcccgctttc
     1981 aagaagtgtg ctaaaagcaa ttcaaagtaa tgaatgatgg aacactgtta tttgaaaaaa
     2041 ggccttggca tgctttttgt catagtctct tgaatgcagt tctagaaaaa tagtgatccg
     2101 aacgataggt tttggcctgt cgttcggatc gctttttctt ttccaactgt attgctcttt
     2161 tattgaagat aatttgctct tcaatagata tttctagaaa ggttctgttt atcccttact
     2221 caaaaaactg ctgcatttgt tctcttactt gctgacacca ttggagttcc tgctgacaaa
     2281 gcataattct gcgctggatc acaacataac gtccaaattc ttgtttcttg tctagcgaga
     2341 ggtcattcaa ttttttttga tttttttgca tgattttgtt cagctgttgt tctctagtga
     2401 atagcagtcc agtagctgtt gttttatcga gcgtcgaaaa agcatagatt tttgctagaa
     2461 attcatcttt ttgcgtagga gcgggagttt cctctttgat ccattcctct acaagagcac
     2521 gtccttcatc agtcaagtgg tagatctttt tttgagagtg ctcgtctttg tccaaaacgg
     2581 tcacataacc ctgctggtct aacttggcaa gcgttgtata gatctgactg tgatgggctt
     2641 cccaaaaaat tgtgatcagc tgcttcaatt catacccaga acgtggtttg gataaaagca
     2701 cgcataataa tatataggct aaagtattca taaaaatcct acctctcgtc ttattttctt
     2761 tattatatca cagtaaagtt tccacgtttt acatgtgcat attgacatgt aaaataaatc
     2821 atgtatgatg ctaatagaga gaaagggatg gaaatattgg agaatcaaat agatatacaa
     2881 gaacaaacaa atgaagaaac aaaggggaaa agttcagcgc tagttgtagc agctacctgt
     2941 atgatcgctt ttatgggagt gggtctagta gacccaatct taaaaacgat tgctgcacag
     3001 ttaaacgcta cgccagctga aacgacc
//
LOCUS       DACDTO010000262         2581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.166, whole genome shotgun sequence.
ACCESSION   DACDTO010000262 DACDTO010000000
VERSION     DACDTO010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.166"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            210..1250
                     /locus_tag="IWK70_003030"
     CDS             210..1250
                     /locus_tag="IWK70_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9615480.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1348..1536
                     /locus_tag="IWK70_003031"
     CDS             1348..1536
                     /locus_tag="IWK70_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9615481.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1594..1947)
                     /locus_tag="IWK70_003032"
     CDS             complement(1594..1947)
                     /locus_tag="IWK70_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9615482.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2478..>2581
                     /locus_tag="IWK70_003033"
     CDS             2478..>2581
                     /locus_tag="IWK70_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007745766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9615483.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLD"
BASE COUNT          867 a          412 c          457 g          845 t
ORIGIN      
        1 atcattttca attgcatttt ataaatcatt ttgtatgtaa tttggtatgt aagttaaaat
       61 gaaaaaagta gtccattttg aagtacattt tgtcattttg tactccaaaa ctgaactact
      121 tacttgcata tatacgcctt tatagtggag tactacattt acgagattcg gttagagtat
      181 cagaaaaaga aaaaataaaa gttacagaaa tgtatacttc aataaatggt gaattatgta
      241 agattgatag agcttattct ggagaaattg ttattttgca aaatgagttt ttgaagttaa
      301 atagtgttct tggagataca aaactattgc cacagagaaa aaagattgaa aatccgcacc
      361 ctctactaca aacaactgtt gaaccgagta aacctgaaca gagagaaatg ttgcttgatg
      421 cccttttgga aatctcagat agtgatccgc ttctacgata ttacgtggat tctacgacac
      481 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
      541 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
      601 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
      661 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
      721 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
      781 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtacg
      841 gtttatacta tagccctgtt agtactccag cagattttcg gatgcttact cctattgtac
      901 tggagcaagc ctttagaaaa gctggaacag aattgttaga gccatatctt agttttaaag
      961 tttatgcacc acaggaatat ctttcacggg catataacga tgctcccaaa tattgtgcaa
     1021 atatcgtaaa tactcaactg aaaaataatg aggtcattat tattggagaa attcctgctc
     1081 gatgtattca agattatcgc aatgatttaa ctttttttac aaatgggctt agtgtttgtt
     1141 tagcagagct aaaaggatat caggttacca ctggcgaacc tgtttgccag acccgtcgtc
     1201 taaatagtcg gatagataaa gtaagatata tgttcaataa aataacttag tgcgttttat
     1261 gttgttatat aaatatggtt tcttattaaa taagatgaaa tattctttaa tatagatttg
     1321 aattaaagtg gaaaggagga gattgttatt ataaactaca agtggatatt gtgtcctatt
     1381 tgtggaaata aaacaagact acgaatacga gtggatacta tacttaaaaa tttcccttta
     1441 tactgcccca aatgtaagaa cgaaacttta attaatgttc aaaaaatgaa tataataaca
     1501 atcaaagagc cagacgccaa gacgcagagc cgataatttg agaaatgaaa ctctcatctt
     1561 atcggctctt tttgtttatc tgaattttac tgactagcct tcaatatttc ttgcttcatt
     1621 gatacctttt gctagtgctt ccattaagga tagttctttg tctgtaaagc tatccatgta
     1681 tttttctacc tgtaaccttc gagtgctttt aatcaaatta tctgtaggta agaaaaattc
     1741 atcaatagat acatggagta atgatacaag atcatacagc acttgtgtac ttgggtgttg
     1801 tcctttgttt tctatatttg ttaaataacg tgggtcaatt tcaatcattg ttcccacttg
     1861 ttcacgagtg agaccccgtt tcattcgagc ctctttaatc gctaaaccaa cggctctaaa
     1921 atcgtattta tcttcttttt tacgcatact aaatcacctc tatacatttt actgttccta
     1981 tataaccata aacaggtata gaaaaacatg ttatatagtt tatgagttcc tatagaacac
     2041 tgtggaattt attaatgcga tgaatccaat ttcaaataga aaggtcgctg attttattta
     2101 cctatacaac tttataaaat ttagtcatac tcctagaaag tagataaaat tggcgactta
     2161 atggagggaa aagtcgccat gagaaagata atctgtcatc tagttctctt aattaagttc
     2221 ttatcaaaaa aacttatcca cctgtactgg atagatatga ccatcaatgt ggttttgcag
     2281 attacataga tagaaaaatc aaagcatatg aacagagaaa acaatctgtt tatgtgcttt
     2341 tttggtcact taataaatat cttttaggat ttctcttcat ttatgcaaca aatcccccgt
     2401 tcgtgtcggg gtttagtatg aagggagata aagaagcaca tgccactccc tttcacgaca
     2461 gaaagggggt gaaaagcatg aaaccatctt cttttcaaga aaccatagaa aatcagtttg
     2521 actatatctg caaacaagtt attgaagatg aacgaaaaga ttacttcaaa catctagaca
     2581 g
//
LOCUS       DACDTO010000263         2550 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.169, whole genome shotgun sequence.
ACCESSION   DACDTO010000263 DACDTO010000000
VERSION     DACDTO010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.169"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            100..1602
                     /locus_tag="IWK70_003034"
     CDS             100..1602
                     /locus_tag="IWK70_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9615484.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            1669..2196
                     /locus_tag="IWK70_003035"
     CDS             1669..2196
                     /locus_tag="IWK70_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9615485.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2407..>2550
                     /locus_tag="IWK70_003036"
     CDS             2407..>2550
                     /locus_tag="IWK70_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9615486.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKR"
BASE COUNT          879 a          439 c          543 g          689 t
ORIGIN      
        1 tttttcgatg tatgggtctt tttgtgtgta tccaaccctt gcatcctctt tagccagttc
       61 tgaaaatagg actggtcttt ttttacgagg tgatggaaaa tgtctaaaat cgaaataaaa
      121 aatctgacat tcggctacga cagccaaggc acattattat ttgaacaagc aaatctaaat
      181 tttgacacac aatggaaact aggacttatc ggacgaaacg gtcgaggaaa gacaacttta
      241 ctgaatattc tacaaaacaa actaccttat caagggcaag taatccatca gcaagaattt
      301 gcctatttcc cgcaacagac aaaagataaa gaacgtttaa cctattacgt gttaaatgat
      361 attacggatt ttgagatatg ggaaatcgaa agagagctcc aattgatgca aacagatcct
      421 gaaatcttat ggagagaatt cagcacacta tcggggggag agaagacaaa agtcctactg
      481 gcacttttat ttgtggatga cactcatttc ccgttaatcg atgaaccaac gaatcatttg
      541 gatatctctg gtagaaaaca agtagcggct tatttgaaaa agaaaaaaca aggcttcatc
      601 gtggtcagcc atgaccgggg atttatcgat gaagtagtgg accatgtttt agcaatcgaa
      661 aaaagtcaac tggaacttta tcaagggaat ttctctatct atgaagaaca gaaaaaactt
      721 cgtgatgaat ttgaaatggc tcaaaatgaa aaattgaaaa aagaagtcag taggctaaag
      781 aaaacagcag ctgaaaaagc cgaatggtct cgttcccgag aaggagataa aacaaagaaa
      841 caagtcggat tcatcgatac tgaatctaga cgagtgaata aaggagcagt gggtgctgat
      901 gctgcacgga cgatgaaacg atccaaagca atcgtgaatc ggatggagac ccagatcagc
      961 gagaaagaaa aactattaaa agatatcgaa tatatcgatt ctttgacgat gaatagccaa
     1021 gcgtctcacc ataagcgact tttaagcgta gaagatcttc aattagggta tgaaaatctg
     1081 ttattcgagc caattcattt tacaatcgag cctcatcagc gggtggcgat ttcaggtcct
     1141 aacggtgcag gaaagtcatc cattatccat tatcttctgg gggcattcaa cggcaaggtt
     1201 ataggagaaa aataccagcc aaaacatctg agcattagtt atgcaagcca aaattatgaa
     1261 gacaatcgag gaacgttggc ggaatttgca gagaaaaacc aagtagacta ccaagcattt
     1321 ttgaacaacc tccgaaagct tgggatggaa agagatgttt ttcataacaa gatcgagcag
     1381 atgagtatgg gccaacggaa aaaagtggaa ttggctaaat ctttatcaca gccagctgaa
     1441 ctatatacat gggatgaacc attgaattat ttggatgtct tcaatcaaga acaattagaa
     1501 caactgatct tgaacgtgaa acctgccatg ttactagtgg aacatgatca aaccttcctg
     1561 gataaagtat ctactgagat tatttctctt gagagaatct aacccttgta atctcaagaa
     1621 caacaaggta tggttaaggg gaactttgcc aacgaaggag agaatcacat gctttatgta
     1681 acgagacacg gagaaacaac ttggaacgca caaggattag tttgcggacg ggcagatgtg
     1741 ccgctgactg aaaaaggaca gatgcaggca cagaaactag cagaaaaggt agtagatttg
     1801 cctgttccaa tcacgaaaat catccattcc cctcttcaac gagcaagaga tacagcacaa
     1861 gcagttgctg atcgtctcag cttgccgctg accgttgatg aacgattggt agaaatggat
     1921 tttggggatt atgatggact gccaagcaaa gatgagaact ttcaaaaagc acgtttagcg
     1981 ttcgctgtac gattcccaaa tggggagtca gtcttagatg tgtatgcacg gatcgttcct
     2041 ttattgaaag aatgcatcga agacgaagaa aatgtctatc tgttagtttg ccataatgcc
     2101 ttgataagag tgatcaatgc ttattttcat ccgatgccta atgaggggtt ctttacattc
     2161 atggtagata atacagaatt ggtttcttat gagtaattat gtttttgtgt tatttaaata
     2221 aaatatttca aggcactgct tttgcttagc agtgcctttt ttaggttatg acaattcttt
     2281 tgataatata aactcaacct ttagaaatga aatttaatta ttttaataaa agtattgaaa
     2341 ttttgtttct ttagatatat actctagtca tagaaatgaa gacggataca gaaaaagagg
     2401 gataatatgt ttggcttttc aatatttatg aacgattctt tgtcagaaga gaagaaacag
     2461 tatatccgcc aaatggcatc caatggcttt gtcggtattt tcacttccat gcatatacca
     2521 gaagatgatg tttcggcata tcaaaagcga
//
LOCUS       DACDTO010000264         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.195, whole genome shotgun sequence.
ACCESSION   DACDTO010000264 DACDTO010000000
VERSION     DACDTO010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.195"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWK70_003037"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWK70_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWK70_003038"
     CDS             394..477
                     /locus_tag="IWK70_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9615487.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWK70_003039"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWK70_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9615488.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDTO010000265         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.205, whole genome shotgun sequence.
ACCESSION   DACDTO010000265 DACDTO010000000
VERSION     DACDTO010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.205"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWK70_003040"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWK70_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWK70_003041"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWK70_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9615489.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACDTO010000266         1194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.220, whole genome shotgun sequence.
ACCESSION   DACDTO010000266 DACDTO010000000
VERSION     DACDTO010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.220"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            259..>1194
                     /gene="vanY"
                     /locus_tag="IWK70_003042"
     CDS             259..>1194
                     /gene="vanY"
                     /locus_tag="IWK70_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9615490.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          173 c          238 g          357 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc cattgtcaac aggcagttca
       61 gcctcgttaa attcagcatg ggtatcactt atgaaaattc atctacattg gtgataatag
      121 taaatccagt agggcgaaat aattgactgt aatttacggg gcaaaacggc acaatctcaa
      181 acgagattgt gccgtttaag gggaagattc tagaaatatt tcatacttcc aactatatag
      241 ttaaggagga gactgaaaat gaagaagttg ttttttttat tgttattgtt attcttaata
      301 tacttaggtt atgactacgt taatgaagca ctgttttctc aggaaaaagt cgaatttcaa
      361 aattatgatc aaaatcccaa agaacattta gaaaatagtg ggacttctga aaatacccaa
      421 gagaaaacaa ttacagaaga acaggtttat caaggaaatc tgctattaat caatagtaaa
      481 tatcctgttc gccaagaaag tgtgaagtca gatatcgtga atttatctaa acatgacgaa
      541 ttaataaatg gatacgggtt gcttgatagt aatatttata tgtcaaaaga aatagcacaa
      601 aaattttcag agatggtcaa tgatgctgta aagggtggcg ttagtcattt tattattaat
      661 agtggctatc gagactttga tgagcaaagt gtgctttacc aagaaatggg ggctgagtat
      721 gccttaccag caggttatag tgagcataat tcaggtttat cactagatgt aggatcaagc
      781 ttgacgaaaa tggaacgagc ccctgaagga aagtggatag aagaaaatgc ttggaaatac
      841 gggttcattt tacgttatcc agaggacaaa acagagttaa caggaattca atatgaacca
      901 tggcatattc gctatgttgg tttaccacat agtgcgatta tgaaagaaaa gaatttcgtt
      961 ctcgaggaat atatggatta cctaaaagaa gaaaaaacca tttctgttag tgtaaatggg
     1021 gaaaaatatg agatctttta ttatcctgtt actaaaaata ccaccattca tgtgccgact
     1081 aatcttcgtt atgagatatc aggaaacaat atagacggtg taattgtgac agtgtttccc
     1141 ggatcaacac atactaattg gttctgttgc aaagttttaa atctactatc aaat
//
LOCUS       DACDTO010000267          872 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20824_2015
            SAMN12639665-rid9882583.guided.237, whole genome shotgun sequence.
ACCESSION   DACDTO010000267 DACDTO010000000
VERSION     DACDTO010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12639665
            Sequence Read Archive: SRR10030725
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000742030.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000742030.1
            Genome Coverage       :: 58x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 04:43:36
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,043
            CDSs (total)                      :: 2,979
            Genes (coding)                    :: 2,908
            CDSs (with protein)               :: 2,908
            Genes (RNA)                       :: 64
            rRNAs                             :: 2, 2 (16S, 23S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 56
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 24 of 71
            Pseudo Genes (incomplete)         :: 36 of 71
            Pseudo Genes (internal stop)      :: 19 of 71
            Pseudo Genes (multiple problems)  :: 7 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12639665-rid9882583.guided.237"
                     /isolate="E20824_2015"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2015"
                     /collected_by="Murdoch University"
     gene            181..678
                     /gene="dfrG"
                     /locus_tag="IWK70_003043"
     CDS             181..678
                     /gene="dfrG"
                     /locus_tag="IWK70_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9615491.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT          326 a           94 c          175 g          277 t
ORIGIN      
        1 tgatttataa aatgcaattg aaaatgataa ttgaatgaaa atttgtgatt ttgtggtatt
       61 atgataatat aacttataga aaaaattgct tattcaacta acgggcagga taatgagaaa
      121 aggttttttg atagaaataa gaaattagat ggtaaattag ttaaaaaggc tgtgttataa
      181 atgaaagttt ctttgattgc tgcgatggat aagaatagag tgattggcaa agagaatgac
      241 attccttgga ggattcccaa ggactgggaa tatgttaaaa atactacaaa gggacatccg
      301 ataatattag gtaggaagaa ccttgaatca atcggaagag ccttacctga cagaagaaat
      361 attattctga cgagagataa ggggtttacc tttaatggtt gtgaaattgt tcattcaata
      421 gaagatgttt ttgagttatg taaaaacgaa gaagaaattt ttattttcgg aggagaacag
      481 atttataatt tgtttttccc ttatgttgag aaaatgtaca tcacaaaaat acatcatgaa
      541 ttcgaaggag atactttttt tccagaagtg aattatgagg aatggaatga ggtatttgcc
      601 caaaaaggga taaagaatga taaaaatccg tataactact attttcatgt atatgaaaga
      661 aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga acagtaaaga taaagcacaa
      721 gagcttggga ttactccaag ctctttttgt catttgaaat aaattatatc tttccaatca
      781 aattgtttat ctgtaatccc taatctttga gcaggtgtca aaaccttttt atccgctgat
      841 ttaaaagggc gacaaaagtt gtagtaagtt ct
//
